LOCUS       DASRDH010000001         6119 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_570329,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000001 DASRDH010000000
VERSION     DASRDH010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 6119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6119
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_570329"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="VFA14_00005"
     CDS             complement(<1..296)
                     /locus_tag="VFA14_00005"
                     /inference="COORDINATES: protein motif:HMM:NF022646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2891 family protein"
                     /protein_id="HZS79808.1"
                     /translation="MATGYWLLLRCARLYPFLPTYGKITAIFDEHFTPALMQQETAYF
                     QTANRASFERPYGFGWILGLAQELTASTHPRAFTWRTAMQTLTMEIRRRLLDYLG"
     gene            complement(208..468)
                     /locus_tag="VFA14_00010"
     CDS             complement(208..468)
                     /locus_tag="VFA14_00010"
                     /inference="COORDINATES: protein motif:HMM:NF022646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2891 family protein"
                     /protein_id="HZS79809.1"
                     /translation="MQLDTALATAYTEMPLTYLRREYPNHIMHVLNGADDVLSPRALH
                     PVFYGCYDWHSAVHGYWLLAAAALRAPVPFSSDLRQDHRHLR"
     gene            complement(496..1482)
                     /locus_tag="VFA14_00015"
     CDS             complement(496..1482)
                     /locus_tag="VFA14_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HZS79810.1"
                     /translation="MTTLITINRIYYLIGFIVMLLVGMTMKDRGNPKRYTTALFWFLF
                     GAIFLFGDIMVDQLGRPLAYRLIGVMVILIALIAGAGRLSTGSYRISSDNERKATSIR
                     FGNWLFLPAILIPVITVLCTVLLKGVSVGGVFLLDQKQLTLAALCVACICALLVGWRL
                     TGGTPLQAVRQSRRLVDSIGWAAILPQMLAMLGGVFVAAKTGDSVQQVVSMFVNSDNL
                     FLVVTIYCIGMALFTMVMGNAFAAFPVMSAGIALPFLIIGHHADPAPLVAIGMYSGYC
                     GTLMTPMAANYNIVPAALLELKDKYQVIKVQIPTALTLLTVNIFLMYFIAFR"
     gene            complement(1479..2207)
                     /locus_tag="VFA14_00020"
     CDS             complement(1479..2207)
                     /locus_tag="VFA14_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007881934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HZS79811.1"
                     /translation="MDSSILLPLIGIPIVVAGFALRFNPLLVVTIAGLATGLAVGMDF
                     GMLLETFGEKFINSRSLATFLLILPIIGLLEHYGLKERAQAWISSIASATSARILMLY
                     FLVREGTAALGLIYLGGQAQTVRPLLAPMAEGAAVNTYGEDLPQHIRDKIKAHSAACD
                     NIAVFFGEDIFIAFGAVLLMDAFLKENGITDIEPLHIGLWAIPTALSALTIHMFRLTR
                     LDASIRRDVDAWKKTDDNPGKAPT"
     gene            complement(2261..3001)
                     /gene="pxpA"
                     /locus_tag="VFA14_00025"
     CDS             complement(2261..3001)
                     /gene="pxpA"
                     /locus_tag="VFA14_00025"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007881936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HZS79812.1"
                     /translation="MQIDLNADLGEGCDHDDTLLTLVSSANIACGWHAGDVGTMRRSV
                     RWAMQNHVAIGAHPSFPDRANFGRSAMQLPPEEIISGMLYQLGALDAIVRAEGAHLTH
                     VKPHGALYNQAAQDVVLANAICTAIRTFNPELAVFGLAGSELICAARQAGLIAIEEAY
                     ADRTYNADGSLVSRTLPGALIENEEQAIAQTLSLIKDKQVTTLDGSSITLNVQTVCLH
                     GDSAHALAFARRIHARLGNEGIAIRTPF"
     gene            complement(3027..4019)
                     /locus_tag="VFA14_00030"
     CDS             complement(3027..4019)
                     /locus_tag="VFA14_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007881937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HZS79813.1"
                     /translation="MMEIIQPGMLTSVQDLGRPGHRRLGISPGGALNSLALSTANLLV
                     GNTVHAAGLEITMGACEIRFTTDTRLALTGDDFDAHLDDKSILPYWSTPVRARQVLKL
                     APTGPHSVQGIRTYLAVAGGIAVANILGSRSTDLKAGFGGHEGRALRKGDRLPIGTPD
                     KMMAGPAFGVRLPQWGVTGDHSKPVPLRVLPGPEFEQFTVASKNMLWQDKWRITPNSN
                     RMGYRLAGPGLERQQHHDLLSHGVMPGVIQVPPSGQPIILMGDAQTTGGYPRIGVVIQ
                     ADMWKLAQAPLNTQLQLQPCNLEQALHAWEEQQTCLDDIRQALAGLPHARQYPK"
     gene            complement(4016..4669)
                     /gene="pxpB"
                     /locus_tag="VFA14_00035"
     CDS             complement(4016..4669)
                     /gene="pxpB"
                     /locus_tag="VFA14_00035"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007881940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HZS79814.1"
                     /translation="MTQPQLHYLGDSALLCSLSAPATLPQQQRIWSIAEQAANWPEVS
                     EAVPGMNNLTLILRRPVVEMDTVSRRVLDIWAHIDTDHIAGREIDIPVVYGGADGPDL
                     DVVARHTGLSSTEIIQRHSSGEYIVYFVGFMPGFAYMGGLDPALATPRRDEPRLSIPA
                     GSIGIGGEQTGIYSLASPGGWQLLGRTSLSLFDPACTPPTLLRPGDRVRFTVERTAK"
     gene            4828..5382
                     /locus_tag="VFA14_00040"
     CDS             4828..5382
                     /locus_tag="VFA14_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007881942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding protein"
                     /protein_id="HZS79815.1"
                     /translation="MNSKPLESTPKTVVGKKLPAKIVSSHHLVSERSAELSELEYALI
                     MAGNAFNRWMMRCMMAAGAKDMTAIEVSLLHHVNHRERKKRLADICFVLNIEDTHVVT
                     YALKKLVKSGYVKREKAGKELLFSATIKGQDLCMRYREVRERCLIKVQADSGIQNQAI
                     GEAARLLRSASGLYDTAARAAASF"
     gene            5398..>6119
                     /locus_tag="VFA14_00045"
     CDS             5398..>6119
                     /locus_tag="VFA14_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HZS79816.1"
                     /translation="MQAVEISAFGAPEVLQLREKPLPQLKSGEVLIRVHAAGVNRPDV
                     VQRQGHYPPPPGASDLPGLEVAGEIVDGEPGGSGFSKGDLVCALVQGGGYAQYCAAPL
                     AQCLPVPAGLTALQAAALPETFFTVWSNVFERAHLSPGETLLVQGGSSGIGVAAIQIA
                     AALGHRVFATAGTDEKCRACEALGAERGINYRTEDFETVVRTATSDRGVDVILDMVGG
                     DYLPREIRILADDGRLAVIALLG"
BASE COUNT         1254 a         1606 c         1987 g         1272 t
ORIGIN      
        1 cctagatagt cgagcagacg cctgcggatt tccatggtca gcgtttgcat ggcggtacgc
       61 catgtgaacg cacgcgggtg cgtggaggca gtaagctcct gcgccagccc gaggatccag
      121 ccgaagccgt aggggcgttc gaacgaggcg cgattggcgg tctgaaaata ggcggtttcc
      181 tgttgcatca gcgcgggcgt gaaatgttca tcgaagatgg cggtgatctt gccgtaagtc
      241 ggaagaaaag ggtacaggcg cgcgcagcgc agcagcagcc agtagccagt agccatgcac
      301 ggcggaatgc cagtcgtagc agccatagaa cactggatgc agcgcgcgcg gcgacagcac
      361 gtcgtcggca ccgttgagca catgcatgat gtggttggga tattcccggc ggagataggt
      421 cagcggcatt tcggtgtatg ccgtcgctag cgcggtatcg agttgcatgg aattcctggt
      481 ggaggctgat cgaaatcagc ggaacgcgat gaaatacatc aggaagatat tgaccgtcag
      541 tagtgtcagg gctgtcggaa tctgcacctt gatgacctgg tatttgtcct tcagttcgag
      601 cagcgctgcc ggcacgatgt tgtagttggc cgccatcggc gtcatcagcg taccgcaata
      661 gccggagtac atgccgattg ccaccagcgg cgctgggtcg gcgtgatggc cgatgatcag
      721 gaagggcagg gcgatgcctg cgctcatgac cgggaaggca gcgaaggcat tgcccatgac
      781 catggtaaac aacgccatgc cgatacagta gatcgtcacc accagaaaca gattgtccga
      841 attgacgaac atgctgacga cctgctgcac tgagtcgccg gttttcgcgg cgacaaaaac
      901 accgcccagc atcgctagca tttgcggcag gatcgccgcc cagccgatcg aatccaccag
      961 ccggcgcgat tggcgcacgg cctgtaaagg cgtgccgccg gtcaggcgcc agcccaccag
     1021 cagcgcgcag atgcaggcga cgcatagtgc ggccagcgtc agttgttttt gatccagcag
     1081 gaagacgccg ccgaccgaga cgcccttgag caataccgtg cagagtacgg taatgaccgg
     1141 aatcagaatg gctggcagga acagccagtt gccgaaacgt atcgatgttg ccttgcgttc
     1201 gttatcgctg gaaatccggt aagagccggt cgacaatctg ccggcaccgg caatcagcgc
     1261 gatcaggatg accatgacgc cgatgagtcg atacgccagc ggcctaccca actgatcgac
     1321 catgatgtcg ccgaacagaa aaatcgcgcc gaacaggaac cagaacagcg cggtggtata
     1381 gcgtttcggg ttgcccctgt ccttcatcgt catgccgacc agcagcatga cgatgaagcc
     1441 aatcagatag taaatacggt tgatggtgat gagtgtcgtc atgttggcgc tttgcctggg
     1501 ttatcgtcag tttttttcca tgcatcgaca tcacggcgga tgctggcgtc gaggcgggtc
     1561 aggcggaaca tgtgaatcgt cagcgcactc agtgcggtcg ggatggccca caggccgata
     1621 tgcagcggct caatgtcggt gatgccgttt tccttgagga aggcgtccat cagcagtacg
     1681 gcgccgaagg cgatgaagat gtcttcaccg aaaaacacgg caatgttgtc gcaggcagcc
     1741 gagtgcgcct tgattttgtc gcgaatgtgc tgtggcagat cttcgccata agtattgacg
     1801 gccgcacctt ccgccatggg agccagcaat gggcgcacgg tttgcgcctg gccgccgaga
     1861 tagatcaggc ccagtgctgc ggtgccttca cgcaccagga aatagagcat caggatacgc
     1921 gccgaagtcg cgctggcgat gctggaaatc caggcctgcg cgcgttcttt caggccgtaa
     1981 tgttcgagca ggccaatgat cggcaggatc agcaggaaag tcgccaatga acggctgttg
     2041 atgaattttt cgccaaaggt ctccagcagc atgccgaaat ccataccgac agccaggccg
     2101 gtggcaagtc cggcgatggt gacgaccaat agtgggttga atcgcagggc gaagcctgca
     2161 acgacgatcg ggattccgat caaaggtagc aaaatcgagc tatccatgtg ctgttttctg
     2221 gcgagaatgc ctgtggttga ttgactggat gacggggaac tcagaatggc gtgcgaatgg
     2281 cgatgccttc gttgcctagg cgcgcatgga tccggcgcgc gaatgccaat gcatgcgcgc
     2341 tatcgccatg caggcagacg gtttgcacat tgagcgtgat gctgctgccg tcgagggtgg
     2401 tgacttgttt gtccttgatc agggacagtg tttgtgcgat ggcttgttct tcgttttcga
     2461 tcagcgcgcc gggcagggta cggctgacca gactgccgtc ggcgttgtag gtgcggtcgg
     2521 cgtaggcttc ttcaatggcg atcaggccgg cttgtctggc ggcgcagatg agttcgctgc
     2581 cggccaggcc gaagacggcc agttcgggat tgaaagtgcg tatggcggta cagatggcat
     2641 tggccagcac cacatcttgc gccgcctggt tatagagcgc gccgtgcggc ttgacgtgtg
     2701 tcaggtgtgc gccttcggcg cgcacgatgg catccagcgc gccaagctgg tacagcatgc
     2761 cactaatgat ttcttcaggc ggcaattgca tggcgctgcg accgaagttg gcgcggtcgg
     2821 gaaagctcgg gtgcgcgccg atggcgacgt gattctgcat cgcccagcgc acgctccggc
     2881 gcatggtgcc gacgtcaccg gcatgccagc cgcaggcgat attggcagag ctgaccagcg
     2941 tcagcagcgt atcgtcatga tcacaacctt cgccgaggtc tgcgttgaga tcgatttgca
     3001 tggtgtgtga ctttattctt tttgtattac tttgggtatt gccttgcgtg cggtaggccg
     3061 gcgagtgcct ggcggatgtc gtccagacag gtttgctgtt cttcccatgc atgcagggct
     3121 tgttccagat tgcagggttg cagttgcagt tgtgtattca gcggcgcttg cgccagcttc
     3181 cacatatcgg cctggattac cacgcctatc ctgggatagc cgccggtcgt ctgggcgtcg
     3241 cccatcagga tgatgggctg gccggatggc ggtacttgaa tcacgcccgg cattacgccg
     3301 tgagagagca gatcgtgatg ttgctgacgc tcgagtcccg gccctgctag acggtagccc
     3361 atgcggttgc tgttgggggt gatgcgccac ttgtcttgcc atagcatgtt tttgctggcg
     3421 acggtgaact gttcgaattc agggccgggc aggacgcgca gaggtaccgg tttgctatgg
     3481 tcgccggtta cgccccactg cggtaagcgc acgccaaaag ctgggccagc catcatcttg
     3541 tccggagtgc cgatcggcag acggtcgcct ttgcgcaagg cgcgtccttc atgaccgccg
     3601 aatcctgcct tgaggtcggt gctgcgcgag ccgagaatat tggcgacagc aatgccgccg
     3661 gcgacggcga gataggtgcg tatgccttgc actgaatggg gaccggtcgg cgcaagtttc
     3721 agtacttgcc tggcgcgtac cggtgtactc cagtaaggta ggatagattt gtcgtcgaga
     3781 tgggcatcga agtcgtcgcc ggtgagcgcg aggcgggtat cggtggtgaa gcgtatctcg
     3841 cacgcgccca tggtgatttc gagtccggca gcgtggacgg tattgccgac cagcagattg
     3901 gcggtactga gcgcgaggct gttcaaggcg ccgccggggc tgatgccgag ccggcgatgt
     3961 ccgggccggc cgagatcctg cacggaggtg agcatgccgg gttggatgat ttccatcatt
     4021 tggcggtacg ctccacagta aaacgcacac gatcgcccgg ccgcagcaag gtgggcggcg
     4081 tgcatgcagg atcgaacagc gacagggagg tacggccaag caattgccag ccgccgggag
     4141 aagcgagtga ataaataccg gtctgctcgc cgccgatgcc gatcgaaccg gcggggatcg
     4201 acagacgcgg ctcatctcgg cgcggggttg ccaatgcggg gtcaagcccg cccatataag
     4261 cgaaaccagg catgaagccg acgaagtaga cgatatattc gccgctgctg tggcgctgaa
     4321 tgatttcagt gctggacaag cccgtatggc gggcaacgac gtcgaggtcc gggccgtctg
     4381 caccaccata gacgacagga atgtcgatct cgcgtccggc gatgtggtcg gtatcgatgt
     4441 gcgcccaaat gtcgagaacc cgcctggata ccgtgtccat ttcaacgact gggcggcgca
     4501 ggatcaaggt cagattattc atgcctggca cggcttcgct gacttccggc cagtttgccg
     4561 cctgttcggc gatgctccag atgcgttgct gttgaggtag cgtggcagga gcagacaagc
     4621 tgcaaagcaa tgcgctatcg cctaaataat gcagttgtgg ttgagtcatt tcgtcttccg
     4681 cccatatgcc gagcgcccgt tcccattcca ttcttgtgga tcatacattc atagaaaaat
     4741 ataaacaata tatcgataaa ttatttaaca ttcctatgtt gcatcgttga taataaccag
     4801 attttcttct caattgaaat taagtttatg aatagcaaac ccttggaaag cacgccgaag
     4861 acggttgtgg gcaaaaaatt accggccaag atcgtctcct cgcatcatct cgtttccgaa
     4921 cgcagcgccg aactgtccga gctagaatat gccttgatca tggcaggcaa cgcattcaac
     4981 cgctggatga tgcgctgcat gatggctgcg ggagccaaag acatgacggc gatcgaggtt
     5041 tctctgctgc atcacgtgaa ccaccgtgag cgtaagaaga ggctggctga catctgcttt
     5101 gtcttgaata tcgaggatac gcatgtcgtg acttatgcac tgaaaaaact ggtcaaatcc
     5161 ggttatgtga agagagaaaa ggccggcaag gaattgctgt tttcggccac cataaaagga
     5221 caggatttgt gcatgaggta cagggaagtg cgcgagcgct gtctgatcaa ggtgcaggcc
     5281 gatagcggta tccagaatca ggccatcggc gaggcggcgc gattgctgcg cagcgcctcg
     5341 ggtttatatg acacggcggc gcgcgcagcg gcttcgttct gacggcgaag acaaaaaatg
     5401 caggcggtcg agataagtgc gtttggcgcg cccgaggtgc tgcaattgcg cgaaaagcca
     5461 ttgccgcagt tgaagagcgg cgaggtgttg atccgggtac atgccgcggg cgtcaatcgt
     5521 ccggatgttg tgcagcgtca gggacattat ccgccgccgc cgggggcttc cgatttgccg
     5581 gggctggagg tggcgggtga aatcgtggat ggtgaacctg gcggcagcgg tttcagcaag
     5641 ggagatctgg tgtgtgcgct ggtgcagggc ggtggttatg cgcaatactg cgcggcgccg
     5701 ctggcgcaat gcctgccggt tcctgccgga ttgaccgcgc tgcaggcggc ggccttgccg
     5761 gaaacctttt ttacggtctg gagcaatgtg ttcgagcgcg cgcatctgtc gccgggcgaa
     5821 acgctgctgg tacagggcgg cagttcgggt atcggtgtgg cggcgatcca gatcgcggct
     5881 gcgcttgggc atcgggtttt cgccactgcc ggcaccgatg aaaaatgccg ggcttgcgaa
     5941 gccttggggg cagagcgcgg catcaattac cggaccgaag atttcgaaac cgtggtcagg
     6001 acggcgacct cggatcgtgg cgtcgatgtg attctggaca tggtcggcgg cgattatctg
     6061 ccgcgtgaaa tcaggatact agctgatgat ggccggctgg ccgtcatcgc tttactggg
//
LOCUS       DASRDH010000002         4024 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_919053,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000002 DASRDH010000000
VERSION     DASRDH010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 4024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4024
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_919053"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="VFA14_00050"
     CDS             complement(<1..536)
                     /locus_tag="VFA14_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013236594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HZS79817.1"
                     /translation="MSKTLIIAEKPSVANDIAKTLGGFTRHDEYFESDEYVLSSAVGH
                     LLEIAVPEEYDIKRGKWSFTHLPIIPPHFALNPIAKTESRLKTLSRLIKRKDITGLIN
                     ACDAGREGELIFRLIAQYAKARQPVQRLWLQSMTPAAIRDGFANLRKDEEMLPLADAA
                     RCRSEADWLIGINGTRAMT"
     gene            complement(825..1295)
                     /locus_tag="VFA14_00055"
     CDS             complement(825..1295)
                     /locus_tag="VFA14_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008333600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HZS79818.1"
                     /translation="MFDVLVYLYETYYRPDACPEPAALVRKLSAVGFAEKEITRALGW
                     LTDLEVANHELADQYPQQTAFSFGIRSYAPQEMDVLGTEAIGFIQFLESAKVLDTVQR
                     EIVIERALAIGDAPVCLENLKIIVLLVLWSQGKEPDGLMFGELFLDENEARLLH"
     gene            complement(1353..2462)
                     /gene="dprA"
                     /locus_tag="VFA14_00060"
     CDS             complement(1353..2462)
                     /gene="dprA"
                     /locus_tag="VFA14_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007881186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HZS79819.1"
                     /translation="MAIDAGASDVAEYADWLRLTQTEGVGVEIARRLLSAFGMPSAIF
                     ATDRDALRAVVTARIADAVLKPPSAMRQDHIERALAWAGEPGHRIVTLADADYPPALL
                     NIADPPVLLYVQGRTELLAAPALAVVGSRNATMQGLQNSERFSEAASRNGLTIVSGLA
                     LGIDAAAHRGGLHGIGSTVAVIGTGIDIVYPARQRALAQRLAEAGCLVSEYPLGMPPM
                     AGNFPRRNRIISGLARAVLVVEAAEQSGSLITARMAAEQGRDVFAIPGSIHSPLSKGC
                     HALIKQGAKLVESVEDMLEELGALPLTSLQQGACVVRPEEDRVLSAMGFDPVDADMLS
                     LRSKLDAAVLQAHLLTLELDGVVECLPGGRYRRVR"
     gene            complement(2480..3283)
                     /locus_tag="VFA14_00065"
     CDS             complement(2480..3283)
                     /locus_tag="VFA14_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS79820.1"
                     /translation="MKNSSIVGLWLLLPVLSAGICAAPLRASEQTRHLSPAIRGGALS
                     PALEMPRLFLPSPLVVDAFQPEKALRIVAQRKGREYFGTGDRIVVQGKLDGVAEFSIF
                     HAGALLRDPVSRAYLGSEFVYAGKASLLISDVDGGHRFLIVDARREITAGDYLLPLSM
                     PSGVLSKNMSPAPVDALIVSIHDGTAYAAQHQIVAINKGVRDGMREGVQLRLCAAADQ
                     RGDKAAQALEVFEEEYGRLLVFRVFDRVSYGLIIQASAPVQVGDRARAP"
     gene            3380..>4024
                     /locus_tag="VFA14_00070"
     CDS             3380..>4024
                     /locus_tag="VFA14_00070"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HZS79821.1"
                     /translation="MNRKLYSLQVLRGIAALIVLLFHYRLYLRANDASGSPVWDALFG
                     WGIIGVDIFFVISGFIMVYTTHYYRSGLDSSKRFLINRAVRILPLYYFGLLIAFILGG
                     AMNTFHDPGKIQNLISALTFTVYKTDITPHYIDDGGMYNIRWTLNYEIYFYLVFALCL
                     LIKHRLITLASWGALATCIIPAVAGFQPTLSVQGYHLVSPLYGLLSNPLILEFLI"
BASE COUNT          883 a         1200 c         1088 g          853 t
ORIGIN      
        1 gtcatggcgc gcgtgccgtt gatgccgatc aaccagtcgg cttcactgcg gcaacgggcg
       61 gcgtccgcca gcggcaacat ttcttcatcc ttgcgcaggt tggcgaaacc gtcgcggatc
      121 gccgccggcg tcatcgattg cagccacagg cgctgtaccg gctgcctggc cttggcgtac
      181 tgcgcgatca gacggaaaat cagttcgcct tcgcgcccgg cgtcacaggc attgatcagg
      241 ccggtgatat ccttgcgctt gatcagcctg gacaaggtct tcaggcgcga ctcggtcttg
      301 gcaatcggat tgagcgcaaa gtgcggcgga atgatcggca aatgcgtgaa actccatttg
      361 ccgcgcttga tgtcgtattc ctcgggcaca gcaatttcca gcaaatggcc cacagccgag
      421 gacagaacgt attcgtcgga ttcgaaatac tcatcgtgcc tggtaaagcc gccgagcgtc
      481 ttcgcgatat cgttcgcgac agagggcttc tcggcaatga tgagagtctt gctcataatg
      541 tgatctcaaa gagaatgcaa aaggaaggca gcttgatgcc ctgcagcacc ggaatgaagc
      601 gcaaaaatag aattcttatt gcatgttggc aataagcttt ctatatggta gcgaatatat
      661 ccatgtattg cgggaaataa taagcgcgtt gcggaataaa cggcaagcgc ggcataggat
      721 tagcatggca atagcagccg ctcattggcg ctgacatctt gaccagccat gaaaaacggc
      781 gccatcatgg acgccgtcgc gttgatgcca ctgttgtatc gacgtcaatg caggagcctg
      841 gcttcgtttt cgtcaagaaa caattcaccg aacatcaggc catccggctc tttgccctgg
      901 ctccacaaga ccagcagtac gatgatcttg aggttttcca gacacacagg cgcgtcgccg
      961 atagccagag cgcgctcgat gacgatctcg cgctgcacgg tgtcgagtac cttggcggat
     1021 tcgaggaact ggatgaaacc gatggcttcc gtgccgagca catccatctc ttgtggggcg
     1081 taactgcgaa taccgaagga aaaggcggtc tgctgaggat actgatcggc aagctcgtgg
     1141 ttggcgactt ccagatcggt cagccagcca agcgcccgag tgatttcttt ttctgcaaaa
     1201 ccaacggcag acaatttcct gaccagagcc gccggttcag ggcaggcatc agggcgatag
     1261 taggtttcgt acagataaac aaggacgtcg aacatagacc ctactttatc gcgaaggttg
     1321 ggacaacaca agtctgcgca tccggccatt tatcagcgca cgcggcgata cctgccgccc
     1381 ggcaagcatt cgacgacacc gtcgagttcc agcgtcagca aatgcgcctg caatacagcc
     1441 gcatcgagtt tgctgcgcaa gctcaacatg tcggcatcga ccggatcgaa acccatcgcg
     1501 ctcaacaccc tgtcctcctc cgggcgcacc acgcatgcgc cttgttgcag tgacgtcaac
     1561 ggcaatgcgc cgagctcttc gagcatatct tccaccgact cgaccagctt ggcaccttgt
     1621 ttgatcaacg catggcagcc tttggacaag ggcgagtgaa tcgagccggg gatggcgaac
     1681 acatcgcgtc cctgttcggc agccatgcgc gccgtgatca gtgagcctga ctgctctgcg
     1741 gcttccacta ccagcacagc acgcgcgagg cccgagatga tgcgattgcg acgtgggaaa
     1801 ttaccggcca tcggcggcat gccgagcggg tattcgctga cgaggcatcc ggcttcggca
     1861 aggcgctgcg ccagcgcgcg ctggcgcgcc ggatagacga tgtcgatacc cgtgccgatc
     1921 acggcgactg tcgaccctat gccgtgcaaa ccgcctcgat gcgcagccgc gtcgatgccc
     1981 agcgccagcc ctgagacaat ggtcagtccg ttccggctgg cggcctcaga aaaccgttcg
     2041 ctgttctgca gtccctgcat ggtggcattg cggctaccga caaccgccag cgctggcgcc
     2101 gccagcaact ccgtgcgccc ctgcacataa agcagcaccg gcggatcggc gatgttgagc
     2161 aaggcgggcg gataatcggc atcggccagc gtgacgatac gatgaccggg ttccccggcc
     2221 cacgcaagtg cgcgctcaat atggtcttgc cgcatcgccg agggtggttt cagcaccgcg
     2281 tcggcaatgc gcgccgtcac caccgcgcgc aaggcatcac ggtctgtcgc aaaaatagcc
     2341 gacggcatgc cgaacgccga caacaggcgc cgggcgattt ctacgccgac accttccgtc
     2401 tgggtaagac gaagccagtc ggcatattcg gccacatcac ttgcaccagc gtcaatcgcc
     2461 atccatgcac tcccgatcgt tatggcgcgc gcgccctgtc ccccacctgc accggcgcgc
     2521 tggcctgtat gatcaagcca taggaaacgc gatcgaatac ccggaaaacc agcagacggc
     2581 catactcttc ttcgaaaact tccagcgcct gcgcggcctt gtcgccgcgc tggtcagccg
     2641 ccgcgcacag ccgaagctgc acgccctcgc gcatgccgtc acgcacacct ttgttgattg
     2701 ccacgatctg atgctgggca gcatacgccg tcccatcatg aatcgacacg atcaaggcat
     2761 cgactggcgc cggtgacata ttcttcgaca acacaccgga tggcatggaa agcggcaata
     2821 aataatcgcc agcggtgatc tcgcggcgcg cgtcgacaat caggaatcga tgtccgccat
     2881 cgacatctga aatcaacaga ctggccttcc ccgcatacac gaactcgctg cccagatagg
     2941 ccctgctgac cggatcgcgc aacagcgctc cagcatggaa aatggaaaac tcggcgacgc
     3001 catcgagttt tccctgcaca acaattctat cgcccgtacc gaagtattcc cggcctttcc
     3061 gctgcgcaac gatgcgcagc gccttctcgg gctgaaacgc atcgacgacc agcggcgatg
     3121 gcagaaaaag cctgggcatt tccagcgccg gcgatagcgc accacctctg atcgcggggg
     3181 atagatgccg cgtctgttcc gatgcgcgca atggcgctgc gcagataccg gcggacagga
     3241 ccggcagcag aagccacagg ccaactatgc tagaattttt cacagatcgc cccttcacgc
     3301 ggcaaatcgc ggcgctattt agccccgatt cctggcagtc taaagctttt ttcgacgatg
     3361 gaatttaaca ggaaattcta tgaacaggaa gctttactct cttcaggttt tgcgtggtat
     3421 cgcggcgctg attgttttac tttttcatta tcgtttgtat ctgcgggcca atgacgcaag
     3481 tggttcgcct gtctgggatg cgctgtttgg atggggaatc atcggagtcg atattttctt
     3541 cgtcatcagc ggatttatca tggtttacac cacccattac tatcgtagtg gtctcgactc
     3601 ctccaaacga ttcctgataa accgcgctgt tcgtatcctc cctctctatt attttggctt
     3661 gctcatcgcc tttattctgg gtggcgccat gaataccttt catgacccgg gaaagataca
     3721 gaacctgatc agtgcgctaa ctttcaccgt ttacaaaacc gatatcacac cccattacat
     3781 cgatgatggc ggcatgtata acatccgctg gacactcaat tacgagattt acttttacct
     3841 ggtttttgct ctgtgcttat tgatcaaaca caggctgata acgctggcaa gctggggagc
     3901 attagcgacc tgcatcatcc ctgcggtggc aggttttcaa cctactttga gtgtgcaggg
     3961 ttatcacctt gtatcgccac tctatggtct attgtcaaac cccctgattc tagagttttt
     4021 gatt
//
LOCUS       DASRDH010000003         2898 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_689833,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000003 DASRDH010000000
VERSION     DASRDH010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_689833"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1481
                     /gene="lon"
                     /locus_tag="VFA14_00075"
     CDS             <1..1481
                     /gene="lon"
                     /locus_tag="VFA14_00075"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007585905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HZS79822.1"
                     /translation="KLERAEKVLEADHYGLEKIKERIIEFLAVRQLVKNPKGSILCFV
                     GPPGVGKTSLATSIAKATGRKFVRLSLGGVRDEAEIRGHRRTYIGSMPGKILQSLTKV
                     GVRNPLFLLDEIDKLGMDFRGDPSSALLEVLDPEQNHTFSDHYIEVDFDLSDVMFVAT
                     SNSFNIPPALLDRMEVIRLSGYTEDEKISIAQRYLLPKQIENNGLKGSEISVGGSAIR
                     DIIRYYTREAGVRSLEREISKICRKVVKILLLKKQEKKVAITSRNLDKFLGVRRFDFG
                     VAEKDNQVGQVIGLAWTEVGGDLLTIEAVTIAGKGNVIRTGTLGDVMKESIEAARSVV
                     RSRARKFGIKSEVFEKNDIHIHVPEGATPKDGPSAGIAMTTALVSVFTGIPVRADVAM
                     TGEITLRGEVLPIGGLKEKLLAAHRGGIKTVLIPEQNVKDLSEIPDNVKNRLEIVPVR
                     WIDKVLEIALERQPLALSEEDAASPPVASKDDGAADPSIVKH"
     gene            1612..1687
                     /locus_tag="VFA14_00080"
     tRNA            1612..1687
                     /locus_tag="VFA14_00080"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1645..1647,aa:Val,seq:tac)
     gene            1735..1811
                     /locus_tag="VFA14_00085"
     tRNA            1735..1811
                     /locus_tag="VFA14_00085"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1769..1771,aa:Asp,seq:gtc)
     gene            complement(1863..>2898)
                     /locus_tag="VFA14_00090"
     CDS             complement(1863..>2898)
                     /locus_tag="VFA14_00090"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZS79823.1"
                     /translation="DQRLSNAELGSLIFQPGFSTSTDVTMIAGRGVGMNAVSNDVASL
                     NGRISIDTVSGKGVSFTIHLPLSLVVMQVVLLELGGQIYGIPSLLVEQMVQMQVDERA
                     RMFHDGSMDWQGSRLPMYYLSALLGEDKGVPLQQTLPILVIKRGHDFIALLVDRIIGS
                     REVVTRNIGPQLTHMHGVVGATILDNGNIVLILNPVQLAQHHSHQKNFRWEDTPVSGS
                     GAAQKTVLVVDDSLTVRHVMQRLLTREGYHVVFATDGINALHQLRNAHPDIALVDIEM
                     PRMNGFDLTRHIRSMESTAVLPIIMITSRTAAKYRERAMELGVNAYLGKPCQDEVLLQ
                     CIRDLIGKDS"
BASE COUNT          649 a          752 c          844 g          653 t
ORIGIN      
        1 ataaactgga gcgcgccgag aaggtgctgg aagccgatca ttacggtctc gagaagatta
       61 aggagcgcat catcgagttt cttgcagtcc gccaactcgt caagaatccc aagggttcca
      121 ttctctgctt tgtgggtcct ccgggagttg ggaaaacttc gctggcaacc tccattgcga
      181 aagcaacggg ccgcaaattc gtccgtctct ccctgggcgg ggttcgcgat gaagccgaga
      241 ttcggggtca tcgccgtacc tacatcggtt ccatgccggg caagattctg caaagcttga
      301 ccaaggtcgg cgtgcgcaat ccgctgttcc tgctcgatga aatcgacaaa ctcggcatgg
      361 atttccgtgg cgatccatcg tcggcgttgc tggaagtgct ggatccggag cagaaccata
      421 ccttctccga ccattacatc gaagtcgatt tcgacttgtc ggatgtgatg ttcgtggcga
      481 cgtcgaattc tttcaacatc ccgccggcct tgctcgaccg gatggaagtg attcgcctgt
      541 ccggctatac cgaagatgaa aagatcagca tcgcacaacg ctatctgctg ccgaagcaga
      601 tcgaaaacaa tggcctgaag ggaagtgaaa tcagcgtggg gggatcggcg attcgtgaca
      661 tcatccggta ttacacccgt gaagctggcg tgcgttcgct ggaacgtgaa atctccaaaa
      721 tttgccgcaa ggtcgtgaag atactgctgc tcaaaaagca ggaaaagaaa gtcgcgatta
      781 cttcacgtaa tctggataaa ttcctgggcg tgcgtcgttt cgatttcggt gtcgcagaaa
      841 aagacaatca ggtcggtcag gtcataggtc tggcatggac tgaggtagga ggtgatttgc
      901 tgaccatcga ggcggtgacg atagccggca agggtaacgt gattcgcacc ggcacgttgg
      961 gcgatgtgat gaaggaatcg attgaagctg cgcgttccgt tgtgcgcagc cgcgcgcgta
     1021 aattcggcat caagagtgaa gtgtttgaga agaatgacat tcacattcac gtgccagaag
     1081 gcgctacgcc aaaagatggt ccgtcggctg gtatcgcgat gacgacagca ctggtgtcgg
     1141 tgtttaccgg gattccggta cgcgccgatg tggcaatgac gggagaaatt accctgcgcg
     1201 gtgaagtctt gccgataggc ggcctgaagg aaaagctgct ggcggcgcat cgcggcggca
     1261 tcaagacagt gttgatacct gagcaaaacg tcaaggatct gagcgagatc cccgacaacg
     1321 tgaaaaacag gctggaaatc gtaccggtgc gctggatcga caaggttctg gaaatcgcgc
     1381 tcgagcgcca gccgcttgcc ttgagtgaag aagatgcggc gtcgccgccg gttgcgtcga
     1441 aggatgatgg ggctgccgac cccagcatcg tgaagcattg attgtagatc ctgaacagcc
     1501 tcttgcatgg cgtctgactc gattcagact tggcaagatt cgcagagatg cgttatactt
     1561 gccgtctttc gacgtggaca gatagtggtc gatagtttgg gcttgaggtg cgggtgctta
     1621 gctcagttgg tagagcagcg cccttacaag gcgcgggtcg ggagttcgag cctctcagca
     1681 cccaccatac ctggcagcgc ttcaaactgc gtaagcgaat tctgtacgat ttttggagtg
     1741 gtagttcagt tggttagaat accggcctgt cacgccgggg gtcgcgggtt cgagccccgt
     1801 ccgctccgcc aatgaagtca acgggttagt gagaaatcac taacccgttt ttctttggag
     1861 gctcaactgt cctttccgat caggtcgcgt atgcattgca gcaaaacctc atcctgacag
     1921 ggcttgccca gataggcgtt gacacccagt tccatggcgc gctcacgata cttggcggct
     1981 gtgcgtgagg taatcatgat gattggtaac acagcagtgc tttccatgct gcgaatatgg
     2041 cgcgtcagat cgaagccatt catgcgcggc atctcgatgt cgaccagcgc gatatcggga
     2101 tgcgcattcc gcaattgatg cagagcgttg atgccatcgg ttgcgaatac gacatggtag
     2161 ccctcgcgtg ttagcaggcg ttgcatgacg tgtctgaccg ttagtgagtc atcaaccacc
     2221 aggaccgttt tttgcgctgc gccgctgccg ctaaccggag tatcctccca ccgaaaattt
     2281 ttttgatggc tgtggtgctg cgccaactgg acgggattga ggatcagaac gatgttgccg
     2341 ttgtcgagta tggtggcgcc gacgacaccg tgcatgtgtg tcagttgcgg cccgatgttt
     2401 ctggtcacga cttcgcgact gccgataatg cgatccacca ggagggcgat gaagtcgtga
     2461 cctcgcttga tgaccaggat cggcaaggtc tgttgcagcg gcacaccttt gtcttctccc
     2521 agcaaggccg acagataata catcggcagc ctgctgcctt gccaatccat actaccatcg
     2581 tgaaacatcc tggctcgttc atccacttgc atttgcacca tctgctcgac cagcaaggac
     2641 ggaatgccgt aaatctgtcc gcccaattcc agcagcacta cctgcatcac caccagcgaa
     2701 agcggcaagt gaatggtgaa gcttacgcct tttcccgaca cagtatcgat actgatacgg
     2761 ccgttcagcg aagccacatc gttgctaacc gcgttcatgc cgacaccgcg tccggcaatc
     2821 atggtgacat ccgtgctggt ggaaaatccg ggctgaaaga tgaggctgcc cagttcagca
     2881 tttgacagac gctggtcc
//
LOCUS       DASRDH010000004         2548 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_918087,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000004 DASRDH010000000
VERSION     DASRDH010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_918087"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1656)
                     /gene="aceE"
                     /locus_tag="VFA14_00095"
     CDS             complement(<1..1656)
                     /gene="aceE"
                     /locus_tag="VFA14_00095"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008327017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HZS79824.1"
                     /translation="MSAQIDQVPAQAANDPDVIETQEWLDALEAVIDAEGTERAHYLM
                     ERMGDLARRRGACIPFSGNTAYVNTIPADQGEHCPGNLEYEERLRSWMRWNAMAMVVK
                     ANRIDGDLGGHLSSFASLANMLGIGFNHFWRAPTADHGGDLLYLQGHSSPGVYARAFL
                     EGRLSEDQLTHFRREADGHGLSSYPHPKLMPEFWQFPTVSMGLGPLMAIYQARFLKYL
                     HARGIADTVNRKVWAFCGDGEMDEPESMGAIGMAGREKLNNLVVVVNCNLQRLDGPVR
                     GNGKIIQELESDFRGAGWNVIKVIWGSDWDELLARDKEGILQKVMMETVDGEYQNYKA
                     KDGAFVRKHFFGKHPKLLEMVSKMSDDDIWRLTRGGHDPHKIYAAFKLAQESRDQPTV
                     ILAKSIKGYGFGKAGEARNTAHNTKKLDDEAIKAMRDRFQLPIPDAELPSIPFFKPAD
                     DTPEMQYLHARRKALGGYLPQRRPQADEKLPVPDLSAFQVMLEPTAEGREISTTAAYS
                     RILTLLLRDPNLGRRIVPIMVDESRTFGMEGLFRQIGIFSQVGQ"
     gene            complement(1981..>2548)
                     /locus_tag="VFA14_00100"
     CDS             complement(1981..>2548)
                     /locus_tag="VFA14_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HZS79825.1"
                     /translation="LGISLQTSRFLDVFLHFIAFEESALTSDESGAENRCNFARVVKQ
                     GRRPGLQLQQEGASIGLQEWGLRLLERMQPIAVLLDMQAGGEQYAQALQAQGDKLRDA
                     GLTPSARVLREVEAAGNSFAAFALNQSRRQAEQFLARPLSAALTEGFNTMAQASIVQQ
                     QLIEHAQTGDFDSFIAAYRADTTGDLSA"
BASE COUNT          557 a          756 c          695 g          540 t
ORIGIN      
        1 ctgaccgacc tgactgaaaa taccgatctg gcggaacagc ccttccatac cgaaggtgcg
       61 cgattcgtcg accatgatcg gcacgatgcg ccggcccaga ttcgggtcac gcagcagcag
      121 cgtcaaaata cgggaatagg ctgccgtggt cgaaatttca cgaccttccg ccgtcggctc
      181 cagcataacc tggaaagcgg acagatccgg caccggcagc ttctcgtcgg cttgcggacg
      241 gcgctgcggc agataaccgc caagagcctt gcgccgcgca tgcagatact gcatttccgg
      301 ggtgtcgtcg gccggtttga agaatggaat gctcggcaac tcggcgtccg ggatcggcaa
      361 ctggaagcgg tcgcgcatgg ccttgatcgc ttcatcatcg agcttcttgg tattgtgcgc
      421 ggtattgcgt gcttcgccgg ctttgccgaa accgtagccc ttgatactct tggccagaat
      481 gacggtcggt tgatcacggc tttcctgcgc aagcttgaag gccgcataaa tcttgtgcgg
      541 atcatgaccg ccgcgggtca aacgccagat atcatcgtcg gacatcttgc tgaccatttc
      601 cagcagcttc ggatgcttgc cgaagaaatg cttgcgcacg aaagcgccat ccttggcctt
      661 gtagttctga tattcgccat cgacggtttc catcatcact ttttgcagga tgccttcctt
      721 gtccctagcc agcaattcat cccagtcgct gccccagatg actttgatca cgttccagcc
      781 ggcgccgcga aaatcggatt ctaattcctg gatgatcttg ccgttacctc gcaccgggcc
      841 atccaggcgt tgcaggttgc agttgacgac aaccacgagg ttgttcaatt tctcacggcc
      901 ggccatacca atcgccccca tcgattccgg ctcgtccatt tcaccatcgc cgcagaaggc
      961 ccagacctta cggttgacgg tatcggcaat gcctcgcgcg tgcaggtatt tcagaaaacg
     1021 cgcctgataa atcgccatca acggacccag gcccatcgag actgtcggga actgccagaa
     1081 ctcaggcatc agctttggat gcgggtacga cgacagaccg tgaccgtcgg cttcacggcg
     1141 gaaatgggtc agttgatctt cactcaagcg accttcaagg aaggcgcggg catacacgcc
     1201 cggcgaagag tgaccctgaa gatacagcag gtcgccgccg tggtctgcgg tcggggcgcg
     1261 ccagaaatga ttgaagccga tacccagcat gttggccaac gaggcgaacg aggacaaatg
     1321 ccccccgaga tcgccatcga tgcggttggc cttgaccacc attgccatcg cgttccagcg
     1381 catccacgag cgcaggcgct cttcatattc gaggttgccc gggcaatgct cgccctggtc
     1441 ggcaggaatc gtgttgacgt aagcggtgtt accggaaaac ggaatacagg cgccgcgacg
     1501 gcgggccagg tcacccatgc gttccatcag ataatgcgca cgttccgtgc cctcagcgtc
     1561 gatcacggct tcgagagcgt cgagccattc ctgcgtttca atgacatcgg gatcgttggc
     1621 ggcttgcgcc gggacttggt cgatttgggc tgacatgctg cctccctctc ctgaattgat
     1681 aaatgacccg gcaagaaaat tttgccaatc aatgatgcac gacctttata ccgggtaaac
     1741 cggggatttt aacaggggac tcccagttct tgaatattga tatttctttt catatgctga
     1801 aaatgatgct gcaaggcggc atagacaggc tatctccgaa aaacattttt atttatctct
     1861 tgagaaatca atacatccag acctggttgc agcgcatcga aaacatgcga aaaaccgata
     1921 ttcgctgaca tcataaataa aagaagcatc cgaatatccg tatgaatcgt tttgtccggt
     1981 tcaagcgctg agatcacccg tggtgtcagc gcgataagca gcgatgaagc tgtcgaaatc
     2041 accggtctgg gcatgctcga tcaactgctg ctgtacgatg gaagcttgcg ccatggtgtt
     2101 gaatccctca gtcagcgcgg cgctcagagg ccgcgccagg aattgctcgg cctggcggcg
     2161 gctctggttc agggcgaacg ccgcaaacga attgcccgcg gcctccactt cccgcagcac
     2221 acgcgcagaa ggcgtcaatc cggcgtcgcg cagtttatcg ccttgcgcct gcaaagcctg
     2281 tgcgtactgc tcgccgccgg cctgcatatc cagcaggacg gcgatcggct gcatgcgttc
     2341 cagcaaccgc aatccccact cctgcaagcc gatggacgcg ccttcctgct gtaattgcag
     2401 accgggacga cgaccttgct tgaccaccct ggcgaagttg cagcgatttt ccgcgccact
     2461 ctcgtccgaa gtcagcgcac tctcctcaaa ggcgatgaaa tgcaggaaca catcgagaaa
     2521 acgcgaagtc tgcaagctaa tcccgagc
//
LOCUS       DASRDH010000005         3235 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_228954,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000005 DASRDH010000000
VERSION     DASRDH010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 3235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3235
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228954"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="VFA14_00105"
     CDS             complement(<1..430)
                     /locus_tag="VFA14_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS79826.1"
                     /translation="MTAEKHTGPVSAGDRVKLFSRDNPTRPLFLAYLLFLFIAYVLYL
                     TAYFMPTPYWGRELMAWATPTVQGLDTAARVAVERQTDPFPAQVVILYCALGMVVLIA
                     GGLLYVCGNREFRESGIRNLIKQEGDGWKSRRKIFLVGLVG"
     gene            850..2130
                     /gene="tyrS"
                     /locus_tag="VFA14_00110"
     CDS             850..2130
                     /gene="tyrS"
                     /locus_tag="VFA14_00110"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HZS79827.1"
                     /translation="MNAVPPISQAPLPLSDQVLEALTITRRGADELLIENEFAQKLAR
                     AEKTGQPLRIKLGLDPTAPDLHLGHTVVLHKMRQLQDLGHTVIFLIGDFTAMIGDPSG
                     RNATRPPLTREQIDINARTYFSQAKLMLDPARTEIRHNSEWCDPFGSRGMIQLASRYT
                     VARMMEREDFTRRFREGVPISVHEFLYPLMQGYDSVALKADLELGGTDQKFNLLVGRE
                     LQKDFGQEPQCILTMPLLEGLDGIEKMSKSKNNYIGITEPANTMFAKVMSISDVMMWR
                     YYELLSFRSSADIAGLRTEIGAGRNPRDIKVALAQEIVARFHSRQAAEDALADFINRS
                     RGGIPDEVPEVSLSGAPLGIGRLLKQAALCASTSEALRMVEQGGVRIDGAVIRDKGLK
                     VEAGAFVVQVGKRRFARVVLAHGADAPGAGEACA"
     gene            2186..2827
                     /locus_tag="VFA14_00115"
     CDS             2186..2827
                     /locus_tag="VFA14_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HZS79828.1"
                     /translation="MKLWSDSFQDGAAIPGEFAFAVPDLKNHVALSANKNPHLAWSDL
                     PAGAKSLVLLCLDPDALARGDDINQESRIVPADWPRVEFFHWSMVDIPTGIDSLAAGQ
                     FSSAITTRGKAGPAIPGDPLSGARHGLNDYTSWFAGDGDMSGDYFGYDGPCPPWNDAI
                     VHRYIFTLYAVDQEHLAVTGKFTGTDVRNALQGHVLAAASITGMYTLNLALAK"
     gene            2824..>3235
                     /locus_tag="VFA14_00120"
     CDS             2824..>3235
                     /locus_tag="VFA14_00120"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HZS79829.1"
                     /translation="MNLRPTTDILLIRHGETDWNIDKRLQGHIDIALNAEGWRQARAL
                     ALVLENEPLDAIYASDLQRARDTAQAIADLQGQAVLIDPALRERSYGGFEGLQHHEIA
                     QRHPAYFAAWRARALDVRYPDGERVAETMREFSGR"
BASE COUNT          676 a          922 c          961 g          676 t
ORIGIN      
        1 cccccacgag tccgacgaga aatatttttc ttcttgattt ccagccatca ccctcttgtt
       61 ttatcaaatt tcgtattcca gactctcgaa actcccgatt gccacagaca tagagcaatc
      121 cccccgctat caacacaacc atcccaagcg cgcaatacag gatgacgacc tgcgcgggaa
      181 aaggatcggt ctgcctttcc accgccacgc gggccgcggt atccagtccc tgcaccgtgg
      241 gcgtcgccca ggccatcagt tcccgccccc agtacggggt gggcatgaaa taggcagtca
      301 ggtaaagaac atacgcaatg aacaaaaaca gaaggtaggc cagaaatagt ggccgggtcg
      361 ggttgtctct ggagaaaagc ttcacccgat cccccgcaga tactgggcca gtatgttttt
      421 cagcagtcat gctctttttt tgatgaggga gttgtttttt acacagcgcc acgcacgata
      481 tcattttgga tcactttctg gcgacagact tcagaagaaa atccgatttt tcgtcaatta
      541 acgaaaactg attcgataga tgatgtgctg cagatcgtga acaggtgctg gtgtcgcacg
      601 caccgcaagt ggtgtagcgg tggctttggc aattgcagtt ggtggattac ggcagctcct
      661 gcgatacaag attccccggc atactgccgg aactgatcat gtccggcagt atgccgctga
      721 ggggatgtag gcgtacaatc atgggtgtcc agacatgggc ttgggcggcg tcacaccgga
      781 gcagtggtta gaccgcctgc tttttacttc tgtatccatc gcaatattgc gtaagtcctg
      841 gttatatcaa tgaacgccgt accgcctata tctcaagctc ccctgcctct gtccgaccag
      901 gttctcgagg cgctgactat caccagacgc ggcgccgatg agttgctcat cgaaaacgag
      961 tttgcgcaaa aactggcgcg cgccgaaaag actgggcagc cgctgcgcat caagctgggc
     1021 ctggatccga ctgcgcccga cctgcatctt ggccataccg tggtgttgca caagatgcgt
     1081 cagttgcagg atctcggtca taccgtgatc ttcctgatcg gtgatttcac tgcgatgata
     1141 ggcgatccgt ccggccgcaa tgcgacgcgt ccgccattga cgcgcgagca gatcgacatc
     1201 aatgccagga cttatttttc ccaagccaag ctgatgctgg acccggccag aaccgagatc
     1261 cgtcacaact ctgaatggtg cgatccgttc ggcagccgcg gcatgattca gctggcgtcg
     1321 cgctataccg tggcgcgcat gatggagcgc gaggatttca ccaggcgctt tagggaaggc
     1381 gtgccgatct cggtgcatga attcctgtat ccgctgatgc agggctacga ctcggtcgcc
     1441 ctgaaggccg atctggaact gggcggcacc gaccagaagt tcaacctgtt ggtcggtcgc
     1501 gagctgcaaa aggattttgg tcaggagccg caatgtatac tgaccatgcc gctgctggaa
     1561 ggcctggatg gcatcgaaaa gatgtccaag tccaagaaca attacatcgg catcaccgaa
     1621 ccggccaaca ccatgtttgc caaggtgatg agtatttccg acgtgatgat gtggcgttat
     1681 tatgagctgc tgtcgttccg ctccagcgct gacatcgccg gcctcaggac cgagatcggg
     1741 gccggcagga atccgcgtga catcaaggtc gcgctggcgc aggaaatcgt ggcgcgtttc
     1801 cattcgcggc aggctgccga agatgcgctg gccgatttca tcaaccgttc caggggcggt
     1861 attccggatg aggtgccgga agtcagcctg agcggcgcgc cgctgggcat cggccggctg
     1921 ctgaaacagg ctgctctgtg cgcgtcgacc tcggaagcct tgcgcatggt ggagcagggc
     1981 ggcgtgcgta tcgatggagc ggtgatccgc gacaagggcc tgaaggtcga ggccggcgcg
     2041 ttcgtcgtgc aggtcggcaa gcgcaggttc gcgcgcgtgg tattggcgca tggcgctgac
     2101 gctccgggtg cgggcgaagc ctgcgcgtga agccggtcgc gtattcactc tgaatcactt
     2161 tgaatgatac aaggagagta ccaacatgaa actctggagc gattcttttc aggatggcgc
     2221 ggcgattcca ggcgaattcg cttttgcggt gcccgatctc aaaaaccatg tcgcgttatc
     2281 ggccaacaaa aatccgcatc tggcatggag cgatctgccc gccggcgcga agtcactggt
     2341 gctgctctgc ctcgatcctg acgcgcttgc gcgcggcgat gatatcaatc aggaaagcag
     2401 gatcgtgccg gccgattggc cgcgcgtcga gttcttccac tggagcatgg tcgatatacc
     2461 gaccggcatc gatagcctgg ccgccggtca gttcagcagc gccatcacca cacgcggcaa
     2521 ggccggcccg gcgattcccg gcgatccgct gtcgggcgcg cgccacggcc tcaacgacta
     2581 caccagctgg tttgccggcg acggcgacat gagtggcgat tatttcggct atgacgggcc
     2641 atgtccgcca tggaacgacg ccatcgtgca tcgttatatt ttcacgctgt atgcggtgga
     2701 tcaggaacat cttgcggtga ccggaaaatt taccggcacc gatgtgcgca atgccctgca
     2761 gggccatgtg cttgccgcag ccagcatcac cggcatgtac acactgaatc tggccttggc
     2821 caaatgaacc tgcgtcccac tactgatatt ctgctgatcc gtcacggcga aactgactgg
     2881 aacatcgaca aacgcctgca aggccatatc gatatcgcac tcaatgccga aggatggcgg
     2941 caggcgcggg cgctagcgct ggtgctggag aacgagccgc tggatgcgat ttatgccagc
     3001 gacctacagc gcgcgcgtga taccgcgcaa gccatcgccg atctgcaggg ccaggccgtg
     3061 ctgatcgatc cagccttgcg cgagcgtagt tatggtggat tcgaggggtt gcagcatcat
     3121 gagatcgcgc aacgccatcc tgcgtacttt gctgcatgga gagcgcgcgc gctggacgtt
     3181 cgctatccgg acggcgagcg cgtggctgaa accatgcgcg aattttccgg acgcg
//
LOCUS       DASRDH010000006         2380 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_682028,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000006 DASRDH010000000
VERSION     DASRDH010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_682028"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1126
                     /locus_tag="VFA14_00125"
     CDS             <1..1126
                     /locus_tag="VFA14_00125"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005669250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HZS79830.1"
                     /translation="KLQDTKAVIPESTFARIYQEVINFCKTNGNFDPTTMGTVPNVGL
                     MAQQAEEYGSHDKTFEIAEAGAANIVDLATGEVLLSQPVEAGDIWRMCQVRDAAIRDW
                     VKLAVTRARHSGMPAVFWLDPYRPHEAELIKKVETYLRDHDTSGLNIQIMSQVRAMRY
                     TLERVIRGLDTISVTGNILRDYLTDLFPIMELGTSAKMLSIVPLMAGGGMYETGAGGS
                     APKHVKQLLEENHLRWDSLGEFLALAVSLEDMGIKTGNRKAQILAKALDDATGKLLDN
                     NQSPSPKTGELDNRGSHFYLALYWAQELAAQKDDAELAARFAPLAKALAENEQKISAE
                     LTSVQGKPADIGGYFLADPEKISAVMRPSATFNAALSSIR"
     gene            1135..>2380
                     /gene="amt"
                     /locus_tag="VFA14_00130"
     CDS             1135..>2380
                     /gene="amt"
                     /locus_tag="VFA14_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HZS79831.1"
                     /translation="MKNKPKFLIAPGIAAISTRVNAQEFATAVTINSGDTAWMMTSAL
                     LVILMSLPGLALFYGGLARSKNMVSLLVQVFAVFCLISVLWLVYGYSLTFGGDGKFWA
                     NLDHSFFMGIGPDTPSRALPNIPEFVWIAFQNAFASLTVALTVGAFAERIKFSAVMVF
                     SVIWFTFCYIPVAHMIWGGGMLADDGALDFAGGTVIHLNAGIAGLVGAYYLGKRNGLG
                     RETFAPHNMTLTTVGASLLWVGWFGFNAGSAGAANGIAGLAFVNTIVATAVAALGWML
                     TEHVIKGKASLLGAVSGAVAGLVGVTPAAGFVGPVGALVIGIICGPACVWGVNGLKRW
                     LRVDDVCDVFGIHGVAGMIGAILTGVFVAPTLGGVGVKDYSMFHQVWVQLKGALMTMA
                     WSAIVTWIAFTTTKFTVGLRVTA"
BASE COUNT          500 a          673 c          678 g          529 t
ORIGIN      
        1 caagctgcag gataccaagg cagtcattcc ggaaagcacc ttcgcccgta tctatcagga
       61 agtcatcaac ttctgcaaga ccaatggcaa cttcgacccg accaccatgg gcaccgtacc
      121 gaacgtcggc ctgatggcac aacaggcgga agaatacggc tcacacgaca agaccttcga
      181 aatcgctgag gctggcgcag ccaacatcgt cgatctggcg actggtgaag tgctactgtc
      241 acaaccagtc gaagccggcg atatctggcg catgtgccag gtcagggacg ccgcgattcg
      301 cgactgggtc aagctggccg tcacccgcgc gcgccactcc ggcatgccgg ccgtgttctg
      361 gctggatcct taccgtccgc atgaagccga actgatcaag aaagtcgaga cttacctgag
      421 ggatcacgac accagcggcc tgaacataca gatcatgtcg caagtgcgcg ccatgcgcta
      481 tacgctggaa cgcgtcatcc gcggcctgga caccatttcg gtgaccggta acattctgcg
      541 cgactacctg accgacctgt tccctatcat ggaactgggc accagcgcca aaatgctatc
      601 gatcgtgcca ctgatggccg gcggcggcat gtacgaaacc ggcgccggcg gttccgcgcc
      661 gaagcacgtc aaacaactgc tggaagaaaa ccatctgcgc tgggattcac tgggcgaatt
      721 tctggcgctg gcagtatcgc tggaagacat gggcatcaag accggcaaca ggaaagcgca
      781 aattctggcc aaggcactcg acgatgccac cggcaagctg ctggacaaca accagtcgcc
      841 atcgccgaag accggcgaac tggacaatcg cggcagccat ttttaccttg ccctgtactg
      901 ggcgcaggaa ctggcggcgc aaaaggacga cgctgaactg gccgccaggt ttgcgccgct
      961 ggccaaagcg ctggccgaaa acgagcagaa aatcagcgct gaactcacgt cggtacaggg
     1021 caaaccggct gacatcggcg gctatttcct ggccgatccg gagaaaatca gcgctgtcat
     1081 gcgtccaagc gcgaccttca atgcggcatt gagcagcatc aggtgatacc tcagatgaaa
     1141 aataaaccca aattccttat tgcgccaggt attgcggcca tcagtacgcg cgtaaatgcg
     1201 caagagtttg ctacagccgt aaccatcaac agcggcgaca cagcctggat gatgacctca
     1261 gcgctgctag ttattttgat gagccttcca ggcctggcac tgttttacgg tggcctcgct
     1321 cgctcgaaga acatggtctc gttgctggtc caggttttcg ctgtcttctg ccttatcagc
     1381 gtcttgtggc ttgtttatgg ttactccctg acatttggtg gcgacggcaa gttttgggct
     1441 aatctggacc actccttctt catgggaatc gggccagata ctccgtcaag agcgttaccg
     1501 aacattcctg agtttgtgtg gattgccttt cagaacgcgt tcgcttcctt gaccgttgcc
     1561 ctgacagtcg gtgccttcgc tgagcgcatc aagttttcgg cggtcatggt gttttcagtc
     1621 atctggttta ccttctgcta tatccctgtc gcccatatga tttggggtgg cggcatgctt
     1681 gctgacgacg gcgctctgga tttcgcgggt ggaaccgtca ttcacctcaa tgcaggtatc
     1741 gctggtctcg taggcgcgta ttacctgggt aagcgaaacg gcttaggaag ggaaaccttt
     1801 gctccccaca atatgacgct aacaacggtc ggagcatcat tgctctgggt tggctggttt
     1861 gggttcaacg caggctcagc tggcgcggca aacggtattg ctggcctcgc ctttgtgaat
     1921 acgattgtcg cgaccgccgt cgctgcactg ggctggatgc tcaccgaaca cgttataaag
     1981 ggaaaagcat cccttttagg tgccgtgtcc ggggcagtcg caggcctcgt gggagtcacg
     2041 cctgctgccg gctttgtcgg tccagtcggc gcacttgtta tcggcattat ctgcggccca
     2101 gcatgcgttt ggggcgttaa tggtcttaag cgttggcttc gtgtggacga tgtttgcgat
     2161 gtgtttggta tccatggggt ggccggcatg atcggcgcca tcctgaccgg tgtattcgtg
     2221 gccccgactc ttggtggcgt aggagtcaaa gactactcca tgttccacca ggtatgggtt
     2281 caactgaaag gcgctctcat gacaatggca tggtcagcca tcgtcacctg gatcgctttc
     2341 actactacca agttcacggt gggtcttcga gtcacagctg
//
LOCUS       DASRDH010000007         4086 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1261802,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000007 DASRDH010000000
VERSION     DASRDH010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 4086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4086
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1261802"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="VFA14_00135"
     CDS             <1..189
                     /locus_tag="VFA14_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016833300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HZS79832.1"
                     /translation="LLTPADSATQCWAFFWFRFFDMLKPPPIAYFDRRFKGGFGVMWD
                     DIVAAFYTLLAFAFWRAM"
     gene            276..764
                     /locus_tag="VFA14_00140"
     CDS             276..764
                     /locus_tag="VFA14_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006462823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HZS79833.1"
                     /translation="MDTSTDLAVRLGQALKARNLLLTSAESCTGGGIAQAITEIAGSS
                     EWFDCGFVTYSNASKHELLDIPEALIAQHGAVSEEIASAMARGAIANSNADIAVSTTG
                     IAGPGGAVPGKPVGTVCFGWKTGDRTLTERLVFSGDRHAVREQAVIHALQGLLRLLNA
                     AD"
     gene            complement(748..1566)
                     /gene="pyrF"
                     /locus_tag="VFA14_00145"
     CDS             complement(748..1566)
                     /gene="pyrF"
                     /locus_tag="VFA14_00145"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008331031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HZS79834.1"
                     /translation="MTFIDQLSAAWKASDSQLCVGLDPDIARFPGPWKNRPDAILAFC
                     QCIIDATADVACAFKPQIACFAAWRAEDQLEEICRYIRTRYPHIPIVLDAKRGDIGTT
                     AGQYAREAFERYGAHAVTVSPYMGFDSVAPYLEWKDRGTIVLCRTSNAGGSDLQFLQV
                     DGMPLYQYVARLVADKWNINGQCGLVVGATFPEELAQVRAIVGDMPLLIPGVGAQGGD
                     VAATVKAGRTTDGVGMMINSSRAILYAAARDDEDHAAAARRVALETRDAINRQR"
     gene            1750..2085
                     /locus_tag="VFA14_00150"
     CDS             1750..2085
                     /locus_tag="VFA14_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HZS79835.1"
                     /translation="MDAIKTLILFIVTALAEIIGCYLPYLWLKKGATPWLILPAALSL
                     ASFAWLLTLHPTVAARVYAAYGGVYVAVALFWMWGVDAVKPNLWDFVGAGMSLAGMGI
                     IMFAPRHGA"
     gene            complement(2236..2418)
                     /locus_tag="VFA14_00155"
     CDS             complement(2236..2418)
                     /locus_tag="VFA14_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007880411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS79836.1"
                     /translation="MTHTVSNNLMLFLPMAFAGLVLVSEAPVASKWARTALRGIGALG
                     GVLVAFLVLEVLPALI"
     gene            2536..>4086
                     /gene="mgtA"
                     /locus_tag="VFA14_00160"
     CDS             2536..>4086
                     /gene="mgtA"
                     /locus_tag="VFA14_00160"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011808203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HZS79837.1"
                     /translation="MNPDPLKASLISFLHARGMERHFRHLGMAIFRNPPVTRNVPRTV
                     MQNLVSASRTDLSELLDKLHTRADGLTEQEANAIRAAVGPNEVEVEKPLTWRAHLWHS
                     YKNPFNLLLTVLAVVSYYTEDMRAAIVIGAMVILSTLMRCIQELRSSNAADKLKEMVS
                     NTATVIRHGITLCRVEVPIRKLVPGDIVQLSAGDMIPADLRLLSAKDLFVNQAAMTGE
                     SLPVEKFAGHHGANAGSPLELYNLCFMGTSVVSGSATAVVLTTGNRTCFGALAERVTA
                     TNRAPTAFQAGVNKVSWLLIRFMLVMTPVVFFINGFTKGDWLEAFLFGLSIAVGLTPE
                     MLPMIVTATLAKGAVALSRKKVVVKRLDAIQNLGAMDVLCTDKTGTLTQDRIFLERHT
                     GILGEPNDAVLQYAYLNSCYQTGLKNLLDVAVLEYAERQRDTAPASSWRKVDEIPFDF
                     QRRRMSVVVSKHEDRHKLICKGAVEEIVSICTHAHNDGQIVALTPVLLAEIRAATAGL
                     NAEGLRVIA"
BASE COUNT          862 a         1293 c         1144 g          787 t
ORIGIN      
        1 ctgctgacgc cagccgattc agcgacgcag tgctgggcgt ttttctggtt ccgtttcttc
       61 gatatgctca agccgccgcc gatcgcttac ttcgaccggc gcttcaaagg cggtttcggc
      121 gtgatgtggg atgacatcgt cgcagcgttc tatacgttgc tggcatttgc cttctggcgc
      181 gctatgtaaa ccgggatcaa caagagtatg ctgacgcgtg ggaacagcag acaataccct
      241 cacatcacac agccagaacg aaagatgaca tcagcatgga cacctcgaca gacctcgccg
      301 tgcgcctggg ccaggcgctc aaagccagaa acctcctgct aaccagcgcc gagtcttgta
      361 ccggcggcgg cattgcccag gccataacag aaatcgccgg atcgtcggaa tggttcgact
      421 gcggcttcgt cacttactcc aatgcatcga agcacgaatt gctggatatt ccagaagcac
      481 taattgccca gcatggcgca gtcagcgaag agatcgcctc tgccatggcc aggggcgcga
      541 tcgccaacag caacgcggac atcgccgtat ccaccaccgg catcgccggc ccaggcggcg
      601 ccgtacccgg caagccggtc ggcacagtct gcttcggctg gaaaacaggc gaccggacac
      661 tcaccgagcg cctggtattc agtggcgacc gccatgccgt gcgtgaacag gctgtgatcc
      721 atgcgctgca aggcttgctg cgcttactca acgctgccga ttgatcgcat cgcgcgtctc
      781 cagcgccacg cggcgcgctg ccgcggcatg atcttcgtcg tcccgcgctg cggcatacag
      841 gatcgcgcgc gaggaattga tcatcatgcc aacgccgtcg gtagtcctgc cagccttgac
      901 ggtcgcggcg acatcgccac cctgcgcgcc cacgccgggt atcagcaagg gcatgtcacc
      961 gacgatggcg cgcacctgcg ccaactcttc cggaaacgtc gcaccgacca ccagtccgca
     1021 ttgaccgttg atattccatt tgtcagccac caaacgcgcc acatactgat acagcggcat
     1081 gccatcgacc tgcagaaact gtagatcaga gccgcccgca ttggaagtac ggcatagcac
     1141 gatggtgccg cgatccttcc attccaaata cggcgctacc gaatcgaagc ccatgtaggg
     1201 gctgactgtg acggcatggg caccgtagcg ctcaaaagct tcgcgcgcat attgcccggc
     1261 ggtggtgccg atgtcgccgc gcttggcgtc gagcacaatg ggaatgtgcg gataacgggt
     1321 acggatatag cggcagattt cttccagctg gtcttctgcc cgccaggccg caaaacaggc
     1381 aatctgcggc ttgaaagcgc aggccacatc ggcagtagca tcgatgatgc attgacaaaa
     1441 agccagaatg gcgtccgggc ggttcttcca tggacccgga aacctggcga tatccgggtc
     1501 gagaccgacg cacagttgcg aatcactggc cttccatgca gcggatagtt gatcgataaa
     1561 tgtcacggcg agccttgaaa tgcaaagagg cgacattgta actgacggag tttaggcagg
     1621 ctgcccgatc aaaaaaacct gcccagtcat ttttctgcca tggcgcgccg atacagcggc
     1681 cctgtgtcgc cccgcccgat ttatattatg atcgggttct gcaacactgc ctcccctctg
     1741 acttcctgta tggatgcgat aaaaacgctg attctcttca tcgtgaccgc cctggctgaa
     1801 atcatcggct gctatctacc ctatctttgg ctgaaaaaag gcgctacgcc ctggctgata
     1861 ttgcctgctg cactcagcct ggcatcattt gcctggctgc tgaccttgca tccgactgtc
     1921 gccgcccggg tatatgccgc ttacggcggc gtctatgtcg ccgtcgcgct gttctggatg
     1981 tggggcgtgg acgccgtcaa acccaacctg tgggatttcg tcggcgccgg catgtcgctg
     2041 gccggcatgg gcatcatcat gtttgcgccg cgccatggcg cctgataaaa aaccgctgcg
     2101 tcattgcgga aatgacgcag cggctgcatc aacgtcaagg gcggaacgac cggctcgtca
     2161 tcgaagccgc ttcatgcaga cttcttccgg ggttccgcca ctgccgcttg cgcggcaaac
     2221 gagctgaaaa gctcgttaaa tcagcgccgg caatacctcc agcaccaaaa acgcgaccaa
     2281 tacgccaccg agggcgccga ttccacgcaa tgccgtcctc gcccatttcg atgcgaccgg
     2341 cgcctcactg accagcacca ggccagcaaa tgccatcggc aaaaaaagca tcaaattatt
     2401 cgagacggta tgcgtcatga ttttgctcct ccaagttttc acttgagtat aggacgctcg
     2461 tcctgtaaaa caaggacaat ttcgcgccga cacatcccgc attgccatgg ataaacacct
     2521 gaaaagagct tgatcatgaa tccggacccg ttgaaagcaa gcctcatcag tttcctccat
     2581 gcgcgcggca tggagcgcca cttccgtcat ctgggcatgg cgatattccg caacccgcct
     2641 gtcaccagga atgtgccgcg aaccgtgatg caaaacctgg tgagcgcgtc ccggaccgac
     2701 ctgtccgaac tgctggacaa actacatacc cgcgccgacg gcctgacgga acaggaagcc
     2761 aacgccattc gcgccgcagt cggtcctaac gaagtcgagg tcgaaaaacc gctgacctgg
     2821 cgggcgcatc tgtggcacag ctacaaaaat cccttcaatc tgctgctgac cgtgctggcc
     2881 gtcgtgtctt actacaccga agacatgagg gccgccatcg tgattggcgc catggtaatc
     2941 ctgtcgacgc tgatgcgttg cattcaggaa ttgcgctcca gcaatgccgc cgacaagctc
     3001 aaggaaatgg tcagcaatac cgcgaccgtg atccgccatg gcatcacgct gtgccgcgtc
     3061 gaagtcccga tcaggaagct ggtgccgggc gacatcgtgc aactgtccgc cggcgatatg
     3121 attccggccg acctgcgcct gctgtcggcc aaggatctgt tcgtcaacca ggccgccatg
     3181 actggcgaat cgttgcccgt ggaaaaattc gccgggcatc acggcgccaa cgccggcagc
     3241 ccgctggagc tgtacaacct gtgcttcatg ggcaccagcg tggtcagcgg atcggccact
     3301 gctgtggtgt tgactaccgg caaccgcacc tgcttcggcg cgctggccga gcgcgtcacc
     3361 gccaccaacc gcgcaccgac ggccttccag gccggtgtta acaaggtcag ttggctgctg
     3421 atccgtttca tgctggtgat gacgccggtc gtgttcttca tcaacggttt caccaagggc
     3481 gactggctcg aggctttcct gttcggcctg tcgatcgcgg tcggcctgac gccggaaatg
     3541 ctgcccatga tcgtcaccgc gacgctggcc aagggcgcgg tcgccctgtc gcgcaaaaag
     3601 gtcgtcgtca agcgtctcga cgccatccag aacctcggcg ccatggatgt gctgtgtacc
     3661 gacaagaccg gcacgctgac gcaggacagg attttcctgg agcgtcacac cggcatcctc
     3721 ggcgaaccga atgatgcagt actgcaatac gcctatctca acagttgcta ccagaccggc
     3781 ctgaagaatc tactcgacgt tgccgtgctg gaatacgccg aacgacaacg cgacacggcg
     3841 cccgcctcat cctggcgcaa ggtcgatgaa atcccgttcg atttccagcg ccgccgcatg
     3901 tcggtggtgg tcagcaaaca tgaagaccgc cataaactga tctgcaaagg cgcggtagag
     3961 gaaatcgtct cgatctgcac ccatgcgcac aacgacggtc agatcgtagc gctcacgccg
     4021 gtgctgctgg ctgagatccg cgccgccacc gccggtctga atgccgaagg tctgcgcgtg
     4081 atcgcc
//
LOCUS       DASRDH010000008         2479 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1491776,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000008 DASRDH010000000
VERSION     DASRDH010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2479
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1491776"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            79..894
                     /locus_tag="VFA14_00165"
     CDS             79..894
                     /locus_tag="VFA14_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006416683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HZS79838.1"
                     /translation="MATKSKFFRVATEGATTDGRTITRTWIQQMATNFNTQKYGARVW
                     LEHLRGIMPDGPFRAYGDVSAVKAEEVEIDGEKRLALYAEIAPTPDLVAMNQAKQKIY
                     TSIEVDPDFANSGEAYLVGLAVTDSPASLGTEVLQFSAKNPEASPFQSRKMRPENLFS
                     AAVETEIEFVEVKDQKTFIQKMKDIVAKLNRKDDEDAERFAQIAESMQQLVEHIDGLD
                     DRFASKQAMEKLQKDHQNLLEKFLKLMEQLENESDPQPKRPFATGTQSQQTDC"
     gene            953..1978
                     /locus_tag="VFA14_00170"
     CDS             953..1978
                     /locus_tag="VFA14_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003267645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HZS79839.1"
                     /translation="MKNDTRKIYEQYSERIAQLNGVPDASKSFSVDPSVQQKLETRIQ
                     ESSAFLSKINIIGVDEKQGEKLGLSISGPIASRTDTATKDRQTRDLSALDKNNYSCQQ
                     TNYDSHIPYARLDAWAKFKDFQNRIRDLIIKRQALDRMMIGFNGISIAADTDIDTNPL
                     LQDVNKGWLQKIREDAPEHFLQEGEKEEDRIIIGTGGDYANLDALVYDLIGMLDPWFR
                     DDTGLVAILGRKMLSDKYFPLINKEQPATEIMATDLIISQKRVGGLSAVTVPYFPEHG
                     ILVTRYDNLSVYFQNGARRRYMVENPKRNRVENYESSNDDYVIEDYGLIAAAENIQIG
                     VTLKEGK"
     gene            1980..>2479
                     /gene="gpM"
                     /locus_tag="VFA14_00175"
     CDS             1980..>2479
                     /gene="gpM"
                     /locus_tag="VFA14_00175"
                     /inference="COORDINATES: protein motif:HMM:NF017731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HZS79840.1"
                     /translation="MARSTLVERHMARVKAEKANQGSEHGKPIVSDAYNLLLGSLAED
                     RRRLKTIQSIERKIEAKAGMLSAYQDWIDSTLKNGKGAQDNIMTTMMIWHIDVGSFDT
                     AISIAEYAIRYDLKLPDEYNRNVATLLIDEFADAALGSKFADPKKALEALRRIGELTE
                     PHDAPD"
BASE COUNT          676 a          720 c          645 g          438 t
ORIGIN      
        1 tgcctcgttg aatgaaacag catcaacctt gaccatgcaa gcatcggccg tatcccattc
       61 aatcaaccga gagaccacat ggcaaccaaa tccaaattct tccgtgtcgc caccgaaggc
      121 gctacgaccg acggccgtac catcacccgc acgtggattc agcagatggc cacgaatttc
      181 aacacgcaga aatacggcgc tcgtgtctgg ctcgaacact tgcgcggcat catgccggac
      241 ggcccgttcc gcgcctacgg tgatgtatca gcggtgaaag cggaagaagt ggagatcgac
      301 ggcgagaagc gcctcgccct gtacgcagag attgcaccga cgcctgacct tgtcgctatg
      361 aaccaggcaa agcagaagat ctacaccagc atcgaagtcg acccggattt cgccaacagc
      421 ggtgaagcct atctcgtcgg ccttgccgtc accgacagcc cagccagcct tggcaccgaa
      481 gtactgcagt tctcggccaa gaatccagaa gccagtccgt tccaaagtcg caagatgcgg
      541 ccggaaaacc tgttctccgc tgccgtcgag acggaaatcg aatttgtcga agtcaaagac
      601 cagaaaacgt tcatacagaa aatgaaggac atcgtcgcca agctgaacag gaaggacgac
      661 gaagacgccg aacgcttcgc tcagatcgcc gaatcgatgc agcaactggt ggaacacatc
      721 gacggtctgg atgacaggtt cgcatcgaag caggcgatgg agaagctaca aaaggatcac
      781 cagaatctgc tcgaaaagtt cttgaagctg atggagcagc tggaaaacga gtcagacccg
      841 cagccgaagc gtccattcgc caccggtact caaagccagc agaccgactg ctgatagccg
      901 aagaccactg taaagcacgc atcgcctacc cattatcgcc atcggagcag acatgaaaaa
      961 tgatacccgc aagatctacg aacagtattc ggaacgcatt gcgcaactga acggcgtgcc
     1021 ggatgccagc aagtcatttt cggtcgaccc atccgtgcaa caaaagctgg aaacccgcat
     1081 tcaggagtcc agtgccttcc tgtccaagat caacattatc ggcgtcgatg aaaagcaagg
     1141 cgaaaaactt ggcctgtcga tcagcggacc gatcgccagt cgcacggata ccgcgacgaa
     1201 agatcgccag acacgcgacc tctctgcgct ggacaagaac aattacagct gccagcagac
     1261 caactacgac tcacacattc cttatgccag gctggacgca tgggcgaagt tcaaggattt
     1321 ccagaatcgc atccgcgacc tgatcatcaa acgtcaggcg ctcgatcgca tgatgatcgg
     1381 cttcaatggc atcagcattg ccgccgatac cgatatcgac accaatccgc ttctgcagga
     1441 tgtcaacaaa ggctggctgc aaaagatccg cgaagatgca ccggaacact tcctgcagga
     1501 aggtgaaaag gaagaagaca ggatcatcat cggcacaggc ggcgactatg ccaatctcga
     1561 cgcactggtc tacgacctga tcggcatgct ggatccgtgg ttccgcgatg acactggtct
     1621 cgtcgccatc ctcggccgaa agatgttatc cgacaaatat tttccgctga tcaacaagga
     1681 acagcctgcc accgagataa tggcgacgga tctgatcatc agtcaaaagc gcgtcggcgg
     1741 tctgtccgcc gtcacggtgc cgtatttccc cgaacacggc attcttgtca cgcgttacga
     1801 caatctgtcg gtctacttcc agaatggcgc ccggcgccgc tacatggtgg aaaatccgaa
     1861 gcgtaaccgt gtcgagaact acgaatcgtc caatgacgac tatgtaatcg aagattacgg
     1921 cctgatcgca gcagccgaaa atatccagat cggcgtaacg ctgaaagagg gcaaataaca
     1981 tggcgcgctc cactctcgtc gaacgccaca tggcgcgcgt gaaagcagag aaggcgaatc
     2041 agggatcgga gcatggcaag ccgattgtct ccgatgctta taacctgctg ctgggatcgc
     2101 tggccgagga tcgtcgccgg ctcaagacca ttcaatccat cgaacgcaag atagaagcga
     2161 aggccgggat gctgtcagcc tatcaagact ggatcgatag cacgctgaag aatggaaaag
     2221 gcgcacaaga caacatcatg accacgatga tgatctggca catcgacgtc ggcagctttg
     2281 atacagcgat ttccatcgcc gaatacgcca tccgctacga cctcaagctg ccggatgaat
     2341 acaaccggaa tgtcgccacg ctcctgatcg acgagtttgc cgatgccgct ctcggcagca
     2401 aattcgccga tccgaaaaaa gcgctggagg cattgcgccg tattggtgaa ctgaccgagc
     2461 ctcacgacgc gccggatca
//
LOCUS       DASRDH010000009         6312 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_338872,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000009 DASRDH010000000
VERSION     DASRDH010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 6312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6312
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338872"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..344)
                     /locus_tag="VFA14_00180"
     CDS             complement(87..344)
                     /locus_tag="VFA14_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS79841.1"
                     /translation="MKITNYTINTNGLAQIAEFLRANHKKGEMIAEDQSMLYAWAADA
                     EFQLDQGNPAMIEIKVWDSIHGRTQTFNISNDGVDSEEINA"
     gene            complement(693..1712)
                     /locus_tag="VFA14_00185"
     CDS             complement(693..1712)
                     /locus_tag="VFA14_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HZS79842.1"
                     /translation="MLTYAIWNNKGGTGKTSLTFQAVCRYAQNNLDKRILTVDMCPQA
                     NISELLLGGLENNGSKNLLKRQGMTPRCSIGGYFDTRMPSAHQLPQFDAGNYITNAHQ
                     HNKFIPKTIDLVCGDPLLEIQAIAMNSLANIQIPGVNTWLTVIDWLRDFLGKIKNDYD
                     VVFIDTNPSFSMYTQIALAATDRIILPVMADDSSRRAIQNAFSLIYGIKLPSPVYEKY
                     TFASKMKEADRQLPKVHLILKNRLTQYMGPASAYAAVLSGIDSDLGNLLKSHNQYFTF
                     DNVSNGVIDIRDFGTTGVAAFACGTPFGVMKTGKLKLGEHCIQVNSEYRDLAIKAVDV
                     IVTKL"
     gene            complement(1736..2059)
                     /locus_tag="VFA14_00190"
     CDS             complement(1736..2059)
                     /locus_tag="VFA14_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014752166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system HicB family antitoxin"
                     /protein_id="HZS79843.1"
                     /translation="MSIDHYTYRVTWSPEDGEHVGLCVEFPSLSWLSATPEEALIGIR
                     QTVASAVADMQVNGERIPEPVAERHYSGEFRVRIPPQLHRMLAMQAAEEGVSLNRLAS
                     AKLAG"
     gene            complement(2065..2307)
                     /locus_tag="VFA14_00195"
     CDS             complement(2065..2307)
                     /locus_tag="VFA14_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013105397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin HicA"
                     /protein_id="HZS79844.1"
                     /translation="MASTKKILEQMRREPTSVRYSDLLKVCEEFFGKPRQSGTSHTIF
                     KTPWPGDPRVNIQDDKGKAKAYQVRQVLMAIDKLME"
     gene            2411..2713
                     /locus_tag="VFA14_00200"
     CDS             2411..2713
                     /locus_tag="VFA14_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003269575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HZS79845.1"
                     /translation="MRIVAKKNLIAFYEKHPVAKQALLAWHEEARAAQWTSPQDIKER
                     YASVSFVRQNRVVFNIKGNAYRLIVAIAYNLGVVYLKFFGTHAEYDKIDAATVELE"
     gene            2718..3140
                     /locus_tag="VFA14_00205"
     CDS             2718..3140
                     /locus_tag="VFA14_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HZS79846.1"
                     /translation="MEIHPIHTEQDYTSALKVIASLVDADPAPGTLEGDRLEILSTLV
                     EHYEAKHFPIELPDPIEAIKFRMEQGNLSVADMQPYLGSAHRVYEVLNRTRNLSLNMI
                     RRLHYGLHIPAEALIGAEPHSQPRSEHNQALDTFPLKR"
     gene            complement(3354..3770)
                     /locus_tag="VFA14_00210"
     CDS             complement(3354..3770)
                     /locus_tag="VFA14_00210"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HZS79847.1"
                     /translation="MTHEGAKSKRARPVPLNDDAMSVLTRQLESVKRLVFTRGEGLDA
                     RIQQIDTRDWERACQAIGIEDFHWHDLRHTWASWHVQRGTPLMVLKELGGWETIEMVQ
                     KYAHLAPSHLAEHAGTVKFWSRLGEQKEKMPLSKAA"
     gene            complement(3943..4617)
                     /locus_tag="VFA14_00215"
     CDS             complement(3943..4617)
                     /locus_tag="VFA14_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009974395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HZS79848.1"
                     /translation="MKKNTLNPAADLLEYLDGKRFSTILADPPWQFQNRTGKVAPEHK
                     RLNRYGTMELDDILNLPVRAAAEDTAHLYLWVPNALLPEGLRVMEAWGFKYKSNVVWH
                     KIRKDGGPDGRGVGFYFRNVTELLLFGVCGKNARTLAPGRSQVNFLATQKREHSRKPD
                     EIYNIIESCSPGPHLELFARGTRPKWVVWGNQADENYHPTWDTYANHSRAEVIQFEQA
                     LKQRQA"
     gene            complement(4598..5398)
                     /locus_tag="VFA14_00220"
     CDS             complement(4598..5398)
                     /locus_tag="VFA14_00220"
                     /inference="COORDINATES: protein motif:HMM:NF020757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglII/BstYI family type II restriction
                     endonuclease"
                     /protein_id="HZS79849.1"
                     /translation="MTANPIDAFIPEDIRTLYEVHNYRNAAQVLATGCPSEFQEIIEG
                     LRAFRIMLADIRKPGGNESDIPKRISGLLREKGWTETRIKGDLLITKISGAGVKKAKA
                     DSGDEEIEIETLEKIEDLKKKGYNVEHIKRENFLDGHKVDYVKHRVAFDLEWNSKDQT
                     FDRDLYAFRTFHECDLIDAAVLLTRGTSLNAVFEKLGPELDNDGKPRIDKNGKPKFIK
                     TKYGASTTWMGKLLYRLNAGRHGGCPVLALGITPNLITDWAAHEKEHS"
     gene            complement(5437..5685)
                     /locus_tag="VFA14_00225"
     CDS             complement(5437..5685)
                     /locus_tag="VFA14_00225"
                     /inference="COORDINATES: protein motif:HMM:NF019493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HZS79850.1"
                     /translation="MIEIPASADAPSWCKMVHLQLVRAMATKEERSAKPKDESKSTRT
                     SVSFPHELYRTLESIAKQKKVTVAWIIRDAAEKYVNEQ"
     gene            5639..5734
                     /locus_tag="VFA14_00230"
                     /pseudo
     CDS             5639..5734
                     /locus_tag="VFA14_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612143.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            5875..>6312
                     /locus_tag="VFA14_00235"
     CDS             5875..>6312
                     /locus_tag="VFA14_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HZS79851.1"
                     /translation="MRFDKLTTKLQEALADAQSQAVGNDNQYIDPVHLVLVLLAQDDG
                     GARSLLQRAGVNVTGLTPALQHAMTRLPKVSGVGGEVQIGRELAALLNLADREAQKHG
                     DQFIASEMVLLGLVDDKSDAGRAARENGLTRKALEAAINAVRGG"
BASE COUNT         1445 a         1627 c         1610 g         1630 t
ORIGIN      
        1 cggcggcgcg actttctcgc acccagcggc ctttcaaagc tgctggaaca cgcaagtgaa
       61 tgagggcatt gctacccgca tttttattat gcattgatct cttcggaatc tacgccatcg
      121 ttagagatgt tgaaagtctg ggtgcgacca tgaatgctgt cccagacctt gatttcgatc
      181 atcgctggat tgccttgatc aagctgaaat tcggcatcgg cagcccatgc gtaaagcatg
      241 ctttgatctt ctgcgatcat ctcgcctttt ttgtggtttg cacgcaggaa ttcggcgatt
      301 tgagcgagac cgttggtgtt gatcgtgtag tttgtgattt tcatgatgtg atcctttcaa
      361 ggaatcgggg agccgcccct gcggtggaca gcatgtgctg tccatatgaa ttattatacg
      421 ctcatttgag cgtaatgtaa gaaatattta ttcgtgcaaa tggatgcttc gtggctaaca
      481 aagcaaagtt gagacgcacc ttggagtagg ccggaaccgc cggaaattcc gtcaggtcgt
      541 ttcggcacaa ccgtagccta tccgatgctg gcaacgggcg ttagtctgaa gccatcaaga
      601 caacaaaaca cttcgcaaga aaaggaaaag ccgtgaggtg attccaactg ctgccagtat
      661 caagtgactg aggcgctagg ctgtaagaat gattacaatt tcgtcacgat gacatcgaca
      721 gccttaatgg ctaaatcacg atattcagaa tttacttgga tacaatgttc gccgagtttg
      781 agcttgccag ttttcattac tccgaatggc gtcccacaag caaatgccgc tactccggtc
      841 gtaccgaaat cacgtatatc gataacgcca ttggaaacgt tgtcgaatgt aaagtactga
      901 ttatgcgact tcagcaagtt gcccaaatcg ctgtcgatgc cagacaatac ggcagcgtag
      961 gcagaagcag gccccatata ctgtgtaagg cgattcttca aaatcaaatg cactttaggg
     1021 agttggcggt cagcttcttt cattttgcta gcaaatgtgt acttttcata tacaggcgaa
     1081 ggaagtttga ttccataaat gagcgagaac gcgttttgaa ttgctcttcg cgatgagtcg
     1141 tccgccatca ccggtaaaat aattctatca gtcgctgcaa gcgcaatctg cgtatacatt
     1201 gaaaaactcg ggttagtatc gataaatact acatcgtaat catttttgat cttgcccaaa
     1261 aaatcacgta accagtcaat tacggtaagc catgtattca ctccaggaat ttgtatattt
     1321 gccagcgagt tcattgcgat ggcttgaatc tcaagcaatg gatcgccgca aacgagatca
     1381 atggttttcg gaataaattt gttgtgttga tgcgcattag tgatgtaatt tccggcatcg
     1441 aactgtggaa gttgatgcgc ggatggcata cgcgtatcaa agtaaccacc gatactacac
     1501 cgaggagtca tgccttgccg tttgaggaga tttttgctgc cattattttc gagaccgcca
     1561 agcagcaact ctgaaatgtt agcttggggg cacatgtcta cggtcaggat tcgcttgtcg
     1621 agattgtttt gcgcgtatcg gcaaacggct tgaaatgtaa ggcttgtttt gcctgtcccg
     1681 cccttgttgt tccagattgc gtatgtgagc attttgtctc ttccttcgag gtgaactacc
     1741 ctgccagctt cgcgctagcg agccgattca aactcacccc ttcctcagcg gcttgcatcg
     1801 ccaacatgcg atgcaactgc ggcggaatac gaactcgaaa ctccccgcta taatgccttt
     1861 ctgcaacagg ctcagggata cgctccccgt ttacctgcat gtccgctact gcggaagcga
     1921 cagtctgccg aatcccgata agagcctctt caggggtagc ggaaagccac gaaagcgaag
     1981 gaaattctac gcacaatccc acgtgttcgc cgtcttcagg cgaccacgta acgcggtaag
     2041 tgtagtggtc gatgctcatt taaattactc catcagtttg tcaatcgcca ttaacacctg
     2101 acgaacttgg tacgccttgg ccttcccttt gtcgtcttga atgttgacac gcggatcgcc
     2161 aggccacggc gtcttgaaga tggtatggct ggtgccggac tgtcttggct tcccgaagaa
     2221 ctcttcacag accttcagca agtcggagta gcgaacgctc gtcggctcac gccgcatctg
     2281 ttcgaggatt ttctttgtgg aagccatggt taaatggtat caataatgac accaaatatc
     2341 aacataaata tatagagaat cacttgtcac gagcgcttgt aatagtccca aaatgggact
     2401 attatgtttt atgcgtattg ttgcgaagaa aaatctgatt gccttctatg aaaagcatcc
     2461 ggtagctaag caggcgctgc tagcgtggca cgaggaagct cgtgcagcac aatggacatc
     2521 gccacaagac atcaaagagc gttatgcatc agtcagtttt gtgagacaaa accgcgtggt
     2581 attcaacatc aaaggaaacg cctaccgact gatcgtagca atcgcgtaca acttaggagt
     2641 cgtctacctc aaatttttcg gtactcatgc ggagtacgac aaaattgacg ccgcgacggt
     2701 agaactggag taacaaaatg gaaattcatc cgattcacac tgagcaagac tacaccagcg
     2761 cgctgaaagt aatcgcgtct ctcgttgatg ctgatccggc tcctggcaca ctagaaggcg
     2821 accgactcga aatcctttca actctggtag agcattacga ggccaagcac ttccctatag
     2881 agcttccaga cccgattgag gctatcaaat tccgtatgga gcaaggaaac ttgtcagtag
     2941 ccgacatgca accgtacctc ggcagcgcgc atcgcgtcta tgaggtgctg aaccgcaccc
     3001 gcaatctcag cctgaacatg atcaggcgct tgcattacgg gctgcacatt cctgctgaag
     3061 ccctgatagg tgctgaaccg cactcgcaac ctcggtctga acataatcaa gcgcttgaca
     3121 cattcccgct gaagcgctga tcgggaacct cactaatgcc cgcgttttgt tccgcaaagc
     3181 aaaaagcacc caagcgggac aagggtttgc gggccgcgat ctgatattga ttgcggaatg
     3241 gatttttgcg taagggactg atatttatag atttcgcggg agtctcttaa tccgtaggtc
     3301 gagtgttcga gccactcacg ccccaccagt aaattacctt gcaaatcaag aagctatgcc
     3361 gctttagaca gcggcatttt ttctttctgc tcccccagtc ttgaccagaa tttgaccgtc
     3421 cctgcgtgtt cggccaagtg actaggagct aggtgggcgt acttttgtac catctcgatc
     3481 gtttcccatc ctcccaactc cttcagcacc atcaatggcg taccgcgctg gacgtgccag
     3541 cttgcccacg tgtgacgcag gtcgtgccag tgaaagtcct cgatgccaat tgcctgacag
     3601 gcccgctccc agtcccgtgt gtcgatttgc tgaatacgag catccagccc ttccccgcgt
     3661 gtgaaaacga ggcgcttcac cgactctagc tggcgagtca gcacactcat cgcgtcgtcg
     3721 ttcaacggca ctgggcgggc gcgcttggac ttggccccct catgcgtgat ctacgcgttg
     3781 cattaggcca aatcgatctg ggccgcatcc agctccaatc aggaaacagg tgtccgagtt
     3841 ggtgagccac tgaagaacgc gttctttggt ttcaccagac attttgcttc tcgatcaagt
     3901 ctgacatttt tggaattgtg tcatttaatc gactgatgac tttcaagcct gacgctgttt
     3961 gagcgcctgc tcgaactgga ttacttcggc gcgtgaatgg ttcgcgtagg tatcccacgt
     4021 cgggtgatag ttctcatccg cctgattacc ccaaactacc cactttggcc gggtgccgcg
     4081 tgcgaatagt tccaagtgcg ggccggggct gcacgattcg atgatgttat atatctcatc
     4141 tggtttgcgg gagtgttcgc gcttctgagt agctagaaaa ttcacttggc tgcggccagg
     4201 cgcaagtgtg cgagcattct tgccgcacac accgaaaagc aacaactctg tgacattacg
     4261 gaaataaaaa ccgacgccgc ggccatccgg cccgccatcc ttacggattt tatgccacac
     4321 aacattgctt ttgtatttga aaccccaagc ttccatgacg cgcagtcctt cgggcagtaa
     4381 tgcattcggc acccacagat acaggtgcgc tgtatcttcg gcggctgcgc ggactggcag
     4441 gtttaagatg tcgtccagct ccatcgtgcc ataccggttc agccgcttgt gttctggggc
     4501 cactttgcca gtgcggttct gaaactgcca tggcggatcc gctaagatcg tactgaagcg
     4561 cttgccatca aggtattcca acagatcggc agcggggtta agagtgttct ttttcatgcg
     4621 cagcccaatc agtgatgagg ttcggtgtaa ttccaagtgc cagcacaggg cagccaccat
     4681 gccgaccggc attgagccga tagagcagtt ttcccatcca tgttgtactg gccccgtatt
     4741 tggttttgat gaatttcggc ttgccgttct tgtcaatacg cggtttgccg tcgttgtcga
     4801 gttccggccc cagtttttca aagaccgcat taagtgacgt accgcgtgtc agcagcaccg
     4861 ctgcatcaat caggtcgcat tcgtgaaagg tgcggaacgc gtagaggtcg cggtcgaaag
     4921 tctggtcttt gctgttccac tccaaatcaa acgcaacccg atgtttgacg taatcgacct
     4981 tgtggccatc aagaaagttt tcgcgtttga tatgctcgac gttgtagcct tttttcttca
     5041 gatcttcgat cttttcgagt gtttcgatct caatctcttc atcgccgctg tctgctttgg
     5101 ccttcttgac gcctgccccg gagatcttgg taatcagcaa gtcacccttg atccgcgttt
     5161 ccgtccatcc tttttcgcgt aggagtccag agatccgttt tgggatatcg gactcgttgc
     5221 cgcctggctt gcggatatca gccaacatga tacgaaatgc gcgcagccct tcaatgattt
     5281 cctgaaattc ggacggacag ccagtcgcca gcacttgcgc cgcgttgcgg taattgtgga
     5341 cttcgtagag cgtgcgaatg tcctctggaa tgaaagcgtc tattgggttc gcagtcatgt
     5401 agcggtggcc tctctctttt ctgcgacgaa caacggctat tgttcattga cgtacttttc
     5461 ggctgcgtcc cggatgatcc aagccacggt aacttttttc tgcttagcga tgctttcgag
     5521 ggtgcggtaa agctcgtgcg ggaaactcac cgacgtgcgt gtgcttttgc tttcatcttt
     5581 tggtttcgcg gaccgttctt ccttagtggc catagctctc accagctgaa gatgcaccat
     5641 tttacaccag ctaggtgcat cggcggatgc ggggatctcg atcaaggaac tgtgccgtca
     5701 gcatggtttc tcggaggcca gctatgacct gtggtgaagc aagtttggcg gcatgagtgt
     5761 gccttacctc gatatcctgc tatcgaggtg tctgcataaa ttagaacgct acaatttcct
     5821 tgaaatatgt ctgcatgtcc caatttcttg ggtattcaag ttgtggagaa aatcatgcgt
     5881 ttcgataaac tcactacgaa attgcaggaa gcgctggctg atgcccaaag tcaggcagtc
     5941 ggcaacgaca atcagtacat cgatccggtc catctggtgc tggtcttgct ggcgcaggac
     6001 gacggcggtg cgcgttcgtt gttgcagcgc gccggtgtca acgtgaccgg gttgactcca
     6061 gccttgcagc atgccatgac gcgcttgccc aaggtctcgg gcgtcggcgg cgaggtgcag
     6121 attggccgcg agctggcggc cttgctgaat ctggctgaca gggaagcgca aaagcatggt
     6181 gaccagttca tcgccagcga aatggtcttg cttggtctgg ttgacgacaa atccgacgcc
     6241 ggccgcgccg cgcgtgaaaa cggtctcacg cgcaaggcgt tggaagcagc catcaacgcc
     6301 gtgcgcggtg gc
//
LOCUS       DASRDH010000010         2635 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_338806,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000010 DASRDH010000000
VERSION     DASRDH010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338806"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="VFA14_00240"
     CDS             <1..321
                     /locus_tag="VFA14_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006712922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2891 family protein"
                     /protein_id="HZS79852.1"
                     /translation="KVLDASAFQHWFDQFLPQIGRIEQLMQPVQVSDRTDPKIAHLDG
                     LNLSRAWCMKRIARCLPATHPAQAALTAAVERHLNTSIGHVIGSHYSGSHWLASFALL
                     ALEA"
     gene            complement(464..1753)
                     /locus_tag="VFA14_00245"
     CDS             complement(464..1753)
                     /locus_tag="VFA14_00245"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007881928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZS79853.1"
                     /translation="MPHVIIIGGGFGGLAAARELADARLDVTLIDRSNHHLFQPLLYQ
                     VATAGLSAPAISAPIRSILAAQLNFTSLMAAVDDIDVARKVVVLEDGSDMAYDYLIVA
                     AGSTHSYFGHDEWAAFAPGLKTLADAFDIRHRILMAFEYAEREDDPERRAAWLTFTVI
                     GAGATGVEMAGTLIEIARHTLRGEFRRIDPRLAKVVLLEGSDHVLGAYPADLSEQARL
                     QLLRLGVDVHTGSRVIDIDAECVRYIDKNGEQRLRSRTVIWSAGVVASPLGKALHAPL
                     DRAGRVVVHGDLSIPDHPEVFVIGDLAAAMSNGKPVPGVSPAAKQMGRVAARNILNRI
                     TGKSTENFSYRDYGSMATIGRKAAVAMVGKLKFSGFSAWLFWLFVHVYFLIGFRNRLV
                     VMSDWAWAYLTFKRSARIMSCAIDDVSPPRQHDEAPT"
     gene            complement(1906..>2635)
                     /locus_tag="VFA14_00250"
     CDS             complement(1906..>2635)
                     /locus_tag="VFA14_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HZS79854.1"
                     /translation="AMGLIKEGNKFAVLTDILGDEDHLGDMDFKVAGTANGITALQMD
                     IKIQGITREIMQVALEQAREGRIHILGKMQEAVPAGKAELSDFAPRLITIRISQEKIR
                     DVIGKGGAVIRALTEETGTQIDISDDGVVTIASVDAAAGQEARRRIEELTACVEIGKI
                     YEGTVLKLLDFGAIVQVLPGKDGLLHISQIANERVNAVADYLKEGQQVRVKVLETDDR
                     GRLKLSMKAAQPEAVGALAAQDAQ"
BASE COUNT          568 a          831 c          718 g          518 t
ORIGIN      
        1 aaggtgctgg acgcgagcgc atttcagcac tggttcgatc agttcctgcc acagatcggc
       61 cggatcgaac aactgatgca acccgtgcaa gtgagcgacc gcaccgatcc caaaatcgcc
      121 cacctggacg gcctcaacct gagccgcgca tggtgcatga agcgcattgc ccgctgtctg
      181 ccagccacgc atcccgcgca ggcggcgctg acagccgctg tcgagcgtca tctgaacacc
      241 agcatcggcc acgtcatcgg cagtcattac agcggtagtc actggcttgc cagcttcgca
      301 ttgctagcgc tggaagctta actggcaggc tgtctcagcc aggcgcggta tacatctaac
      361 agtctctacc ccaagagccg ccaacacaac gccgatggca aggcgcgccg acaaaggtac
      421 ggcaaggagg cgcaacgcag ccagcagtgt tgtgttggcg gctctaagtc ggagcctcat
      481 catgctgccg cggcggcgat acatcatcga tggcgcagga catgatccgc gcactgcgct
      541 tgaacgtcag atacgcccac gcccaatcac tcatcaccac cagacgattg cgaaagccga
      601 tcaaaaaata cacgtgtacg aacagccaga acagccaggc tgaaaagcct gaaaatttca
      661 gcttgccaac cattgccacc gcggccttgc ggccaatggt cgccattgag ccgtaatcac
      721 gataggaaaa gttttcggtc gattttccgg tgatgcggtt gaggatattg cgcgctgcta
      781 cccgccccat ctgcttggca gccggcgaca cgcccggcac cggtttaccg ttagacatag
      841 cggcggcgag atcgcctatg acgaagactt ccggatggtc gggaatcgac agatcaccat
      901 gcaccaccac ccggcccgct ctgtcgagcg gtgcatgcag cgcttttccc agcggcgaag
      961 ccaccacacc tgcagaccag atcaccgtgc gcgacctcag ccgctgctcg ccattcttat
     1021 cgatatagcg cacgcattcc gcatcgatgt cgatgacgcg gctgccggta tgcacgtcaa
     1081 caccgagcct gagcagttgc aggcgcgcct gttccgataa atcggcagga taggcgccca
     1141 gaacgtgatc ggagccttcc agcaacacga ccttcgccaa tcgcggatcg atgcggcgaa
     1201 actcgccgcg taaagtgtgc cgggcgattt cgatcagcgt gccggccatt tcgacgccag
     1261 tcgcgccagc cccgatgaca gtgaaggtca gccatgcggc gcggcgctcg ggatcgtctt
     1321 cacgttcggc atattcaaag gccatcaaaa tccggtgccg gatgtcgaag gcatctgcca
     1381 gtgttttcag tcccggcgcg aacgccgccc attcgtcatg gccaaaataa ctatgcgtgg
     1441 aacccgccgc cacgatcaga taatcgtagg ccatgtcgct accgtcttcc agcaccacca
     1501 ccttgcgtgc gacgtcgatg tcatcaacgg ccgccatcag actggtgaaa ttgagctgcg
     1561 cagcgaggat gctgcgtatc ggcgcgctga tcgcgggagc cgacagacca gccgtggcga
     1621 cctggtacag cagcggctga aacaaatgat gattactgcg atcgatgagt gtgacgtcga
     1681 gcctggcatc ggccagttcg cgcgcggcgg caaggccacc gaaaccgccg ccgataataa
     1741 tcacatgagg catagcattc ccttcctgat gcaaatacag atgattggaa taattatttt
     1801 ctatcgggca ataaaaatct ctcaagacct tcgccttgag agattccata ctacctgatg
     1861 cacacaggca gatacgcgct acccgcatcc gcccgcaatc acctgttact gcgcatcctg
     1921 cgcagcaagc gctcccacgg cttccggctg cgccgccttc atcgacaact tcaggcgacc
     1981 gcggtcgtcg gtttccagca ccttgacgcg gacctgctgg ccttccttca ggtaatcggc
     2041 aacggcattg acacgctcgt tggcgatctg gctgatgtgc agcaaaccat ccttgccggg
     2101 cagaacctga acgatcgcgc caaagtccag cagtttcagc acggtgcctt catagatctt
     2161 gccgatttcc acacaggcgg tcagttcttc gatacgacgc ctggcttcct gaccggcggc
     2221 ggcatcgacg gaagcgatgg tgaccacacc atcgtcactg atgtcgatct gggtgccggt
     2281 ttcttcggta agcgcgcgga tgacggcgcc gcctttgccg atcacatcac ggattttttc
     2341 ctgactgatc ctgatggtga tcaggcgtgg cgcaaaatcc gacagttcag ccttgccggc
     2401 gggaacggct tcctgcatct tgccaaggat atggatacga ccttccctgg cttgctccag
     2461 cgcaacctgc atgatttccc tggtgatgcc ctggatcttg atatccattt gcagcgcggt
     2521 aatgccgttg gccgtacccg ccactttgaa atccatgtca ccgaggtgat cttcatcgcc
     2581 cagaatatcg gtcaggacgg cgaacttgtt gccttccttg atcaggccca tggcg
//
LOCUS       DASRDH010000011        10686 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_338771,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000011 DASRDH010000000
VERSION     DASRDH010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 10686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10686
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338771"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1175)
                     /locus_tag="VFA14_00255"
     CDS             complement(<1..1175)
                     /locus_tag="VFA14_00255"
                     /inference="COORDINATES: protein motif:HMM:NF012635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HZS79855.1"
                     /translation="MLTLVSSLRENEQRMPIKESASLFLIWMVLTLVGLLSCIAAQAK
                     CKRGVYPTSPATDNGVSKLSFGRINITSMVLQHAGTILGSTVATAGNAVGINDETVLW
                     RCDLADKDQLYEVFSTNGDDQFGGFHQIGLHDGMPGYYQTFFSYAAIKITHVRSGKVF
                     SRYWQYEKLTNYDVEGNKILIRAKHLSPVQAELARVSTRKLLGGHSWCGNRMADERGG
                     SPPYIYNCGAKTQPNAYITFSGPGYPADREGSDHATHHRFWPQNGVAFGMRNAAWVSY
                     EPTCIVRNTTPEVTFWPITVQEFQWGEKREVDFSIQLECDHNVNSGTGQNQIAMGIQV
                     SEEAYIKAMELGLVSSMGGVTHLLSNNYGVDPAIATGVGIRIRDAGSGQPMNFLGWQ"
     gene            complement(1311..2093)
                     /locus_tag="VFA14_00260"
     CDS             complement(1311..2093)
                     /locus_tag="VFA14_00260"
                     /inference="COORDINATES: protein motif:HMM:NF012565.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HZS79856.1"
                     /translation="MKTLPPFFLTRRRHALLVKVVLLCLAGMHCLQASAGIMVERTRL
                     IFEEGQQERSLTLANTNDYPIIVQTWVDNGEGSSTPDAVVSAMMPLPGVFRLQPNEIM
                     HLRVLYANDPLPGDRESVFWLNLYEIPLLSSDRHVEQARLLVGINTQIKIFYRPKNLL
                     SSSGDVGRQLAFRLEKTGDGWVLVCHNRSPYFASFASLNVTVGDRHIEAAQSPDMMTP
                     PMSERRYSLSSPVSDVVAGGDVSYILIDDSGNFQRGSAALPR"
     gene            complement(2090..3355)
                     /locus_tag="VFA14_00265"
     CDS             complement(2090..3355)
                     /locus_tag="VFA14_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017454428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HZS79857.1"
                     /translation="MLVGLLSCVAAQARCERSNSPYTPVLDAGSSQLRFGRINITSTI
                     LQPVGTILGSTVATAGNAVGINDETVLWRCDLADKDQLYEVFSTNGDDRVGGYWDIGA
                     KDGLPDYYQTWFSFAAIKITHVRSGKVFTRHWQYTKLTNYDVKDNKILIRAKHLSPVQ
                     AELARVSTVQLGGVSNYCGNNMANGRGSSPWNYSCTQPNAYIAFSGPGYPADPEGSDH
                     AYNYRFWGDRNGVAFGMRNAASVSYEPTCVVRNTTPEVTFWPITIQEFQRGEIREADF
                     SIQLECDRNVKSGTGRNQTAMGIQVSVAAYAKAMELGLVDSMGGVTHLLSTNYGVDPA
                     IATGVGIRIRDADSGQPMNFLGWPGDADPGTGAGAGWYPVFSGGVPSGSGKTVNYTRV
                     ITASLEKLPGEVITPGRVYAIAYVIVKVQ"
     gene            complement(3421..5154)
                     /locus_tag="VFA14_00270"
     CDS             complement(3421..5154)
                     /locus_tag="VFA14_00270"
                     /inference="COORDINATES: protein motif:HMM:NF012786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HZS79858.1"
                     /translation="MLPESQRGYAPVIRGVARTNAQVSVHQNGQEIYRTTVAPGPFEI
                     SDLYPTSYNGDLDVTVLEADGTRSQFSVPFAAVPESMRPGASRYSFTLGRTRDAAAKD
                     LFADVTYQYGLSNSLTPNGGLRISNGYQAIAMGVTHVSVLGALGLNTAYSHAMLAQGK
                     RANGWMAGVTYSKTFQRTDTTFSLASYRYSTQNYRELGDVQSERRYQLDSGIGLSSSY
                     RQRSRFDISLNQRLGKAGSLYLSGLTQDYYGSSRRVTQLQAGYTVVFENDVSLSLAFQ
                     RQRVFSGQFAGAGFLESTENRESSVVVTVFFPLGPRGSDSRPFFSSSVNRANGENTYQ
                     ASISGTLGQGQSMNYGLDVNYAHDGQNAIGGNLHKQFANVSMGINASHGPGYWQAAAS
                     ARGAVAIHSGGITFGPYLSDTFALIEAKGATGAKVFNGQSTAIDSNGYALMPSLVPYQ
                     RNQVGLMPEGMNSKVELNDGQRQVVPYAGAAIKVQFKTRSGNALLIKALREDGNPVPI
                     GADVLDEQGVIIGMVGQGSQAYVRTGNMHGKLILRWGEAAEEQCELPYDLQEEDQQQP
                     LLKLKGVCESR"
     gene            complement(5118..6056)
                     /locus_tag="VFA14_00275"
     CDS             complement(5118..6056)
                     /locus_tag="VFA14_00275"
                     /inference="COORDINATES: protein motif:HMM:NF025320.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
                     /protein_id="HZS79859.1"
                     /translation="MITVAKLKTSIVSSNGACFGFSRGRRVASCLVLRSLPWLLLSLY
                     GFVVQAQAAVGAGKNEAEPVDAKGAYLFIEGLALGSLANRGSLSRFNRSDTIEPGTYD
                     VDLYVNGRLYDRQNIRFGDVDGTTRACLSGDVLTGIGIAEDIMAAAEKNTDCQFISSV
                     VAGATERFDFSHLRLDLSIPQIAMNTRPRGYVDPKRFESGDAIGFVNYNASQYYASTT
                     VSSQNSTYVSTNSGLNLGLWRLRQQGSMSYDPSNGYQWNSVRTYVKRALPGIRGELSF
                     GENYTSGVFFSGVAYQGIELAWTIGCCRNRSVGMRR"
     gene            complement(6013..6762)
                     /locus_tag="VFA14_00280"
     CDS             complement(6013..6762)
                     /locus_tag="VFA14_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007879694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HZS79860.1"
                     /translation="MSNMKKIILKYALMFCAALVSASPAHASVIMVGTRVVYPASLKE
                     RVLNFTNQDNYPNLVQIWVDQGNGSSGSESEDPPFVAHPPVFRMEPQAGQMVRLTYVG
                     ETLPQDRESLFFLNFLQVPAIKASDMEANKLVLTVRSRMKIFYRPTALSNESSMSTMR
                     KSLSFEASRADGVVRVVGRNSSGYYAVLQSVIIASDKGDVPVAEAVLIPPKGEVVWPV
                     KTMTGEVSASRLKVRLINDYGGEIEDEYRFK"
     gene            complement(7239..7766)
                     /locus_tag="VFA14_00285"
     CDS             complement(7239..7766)
                     /locus_tag="VFA14_00285"
                     /inference="COORDINATES: protein motif:HMM:NF012635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HZS79861.1"
                     /translation="MKKVVLLLTAMPSLVFAAGNTIRFQGQVSDQTCHVMVEGAMGPL
                     VVLPTVATSSLAAAGAVAGERPFTLTVTGCKAPVTDVMSIKTAFAVASGATDGGNIPN
                     LGTASNVALQLLDQPGGSPIKLVNGTATSVEGLKLGRGETAASHIFAVRYIAEGAAVG
                     AGSVMGAVQYALDYL"
     gene            complement(8069..8605)
                     /locus_tag="VFA14_00290"
     CDS             complement(8069..8605)
                     /locus_tag="VFA14_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006462262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZS79862.1"
                     /translation="MSTERLLLIIEDDMAFARTLSRSFERRGYVVLLATTQSEAAALL
                     AQHNPGYAVVDLKLNGNTSGLACVQMLHQHDEAMLIVVLTGFASIGTAVEAIKLGACQ
                     YLAKPSNTDDIEIAFGHVAGSTEIELTTRSTSIKTMEWECIHEVLADTDFNISEAARR
                     LGMHRRTLARKLEKQRVK"
     gene            complement(8574..9929)
                     /locus_tag="VFA14_00295"
     CDS             complement(8574..9929)
                     /locus_tag="VFA14_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZS79863.1"
                     /translation="MPDRTVGHKSMLQLIQLRWVAVLGQAGTIIVAVFSFDIQLPLPH
                     MLGVLACLIAFNIGSYMRWHERLFVTNCELFLALLVDVATLTAQLYFSGGTTNPFVFL
                     YLLLIIISAVLLEVWSTWSIVCTTSLCFAGLSQFFIPLSLALDRSAGLLSSRYVEGML
                     LCFIMVSILLVTVITRISGNRRIADAQLATLRQRAVEEDHIVRMGLLASGAAHELGTP
                     LAMLSVILGDWRHMPEFSSNSVLLEEIDEMQIQLQRCKAIVSGVLLSAGEWRGASSLR
                     TSLNTFLSDIMEEFRTTRPIVSFEYDNRIERDMSVIFDSTLKQMIYNVLDNALEVSPY
                     WLRLTVVREGGMLRLTVDDIGPGFVTGMLAQLGQPYQSTKGKIGGGLGLFFVMNAARK
                     LGGTVTAHNREENGRVVGATVCLTLRLSSISFEVDAQKNTAEQGKGNRQYVDRAPVVD
                     H"
     gene            complement(10061..>10686)
                     /locus_tag="VFA14_00300"
     CDS             complement(10061..>10686)
                     /locus_tag="VFA14_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009668043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SURF1 family cytochrome oxidase biogenesis
                     protein"
                     /protein_id="HZS79864.1"
                     /translation="ADPVAAPGPDEWSHISASASADAYEYLHVRLSGTFLYAQTVKVR
                     AVTRLGSGFWLLTPLRGPDGMVTLINRGYIAADAHDIRQAASDAPARLTGLLRISEPS
                     GGFLRNNDPATGRWFSRDVQAIAAAHKLVGKVAPYFVDADVTAAADVAMTEEMRAAAG
                     NPVGGLTVIVFHNNHLVYILTWYGLALMTVYAGYWVMRDENRQRSQI"
BASE COUNT         2672 a         3093 c         2588 g         2333 t
ORIGIN      
        1 tcctgccatc caagaaaatt catcggctgc ccactaccgg catcgcggat gcggatgccg
       61 accccggtag cgatcgccgg atcgacacca tagttgttcg acagcagatg cgtgacgcca
      121 cccatgctgc taaccagccc aagctccatc gccttgatat aggcttcctc agatacctgt
      181 atgcccatgg cgatctgatt ttgtcccgta cctgaattga cattatggtc gcattcaagc
      241 tgaatggaaa agtcaacttc gcgtttttca ccccattgaa attcttgaac cgtaatgggc
      301 cagaatgtca cttcgggcgt cgtattgcgc acgatgcatg tcggctcata gctgacccac
      361 gccgcattcc gcatcccgaa agcgacaccg ttttgaggcc agaaacgatg atgagttgcg
      421 tggtcgctgc cttcgcgatc ggcaggatac cccggcccgc tgaaagtaat ataggcattg
      481 ggttgcgttt tcgcaccaca gttataaatg tagggagggg aacctcctct ctcatctgcc
      541 attcgattgc cgcaccaact gtgtccgcca agcaattttc tggtcgatac ccgcgcaagc
      601 tcagcctgta ccggactcaa atgttttgcc ctgatcagga tcttgttgcc ttccacatcg
      661 taattcgtga gtttctcata ctgccagtaa cggctgaaca ccttgcccga tctgacatgc
      721 gtgatcttga tggccgcgta agaaaaaaag gtttggtaat aacccggcat gccatcatga
      781 agaccaattt ggtgaaaacc gccgaactgg tcatcgccat tggtggaaaa gacctcgtag
      841 agttgatcct tatccgccag atcgcacctc cacagcacgg tttcgtcatt gatgccgaca
      901 gcattgcctg ccgtcgcaac cgtcgatccc agtatggtgc cggcatgctg caataccatg
      961 ctggtgatat tgatccggcc aaacgataac ttggatacgc cattatctgt cgcgggactc
     1021 gtgggatata caccgcgctt acactttgcc tgcgcggcaa tgcaagacag cagaccgaca
     1081 agcgtgagta ccatccagat cagaaatagg ctagcgcttt cttttattgg cattctctgt
     1141 tcattttctc tcaacgacga aaccaacgtc agcaatggta ttacggtctg atttggttcg
     1201 taccagaaaa gtgaattgct gtcacgtttg cctctcccat tcaatatatc cagcgatcgt
     1261 gtaaattgtt tagcgcgtgt cagaaatttc cagaacgccg aagcatagtt tcatcggggc
     1321 aaagctgccg atcctcgctg aaaatttccc gagtcgtcga tcagaatata actgacatcg
     1381 ccgccggcga ccacgtcgga cacgggactg ctcagagaat agcgccgctc gctcatgggc
     1441 ggcgtcatca tgtccggcga ttgcgctgcc tcgatgtggc gatccccgac agtcacgttg
     1501 agcgacgcga acgaagcaaa atagggactg cgattgtgac ataccagcac ccagccgtca
     1561 cccgttttct ccagacgaaa cgccaactgc ctgccgacat ccccggacga ggacagcaaa
     1621 ttcttcggac gatagaaaat cttgatctgg gtattgatac cgaccaataa tcgtgcctgc
     1681 tccacatgac ggtcagaact caacagagga atttcgtaca ggttgagcca aaaaaccgat
     1741 tcgcgatctc caggtaaggg atcattcgca taaagcacgc gcaaatgcat gatttcattc
     1801 ggctgtaaac gaaacacgcc aggcaacggc atcatggccg agaccaccgc atcgggcgtg
     1861 ctgctcccct cgccgttgtc cacccaggtt tgcacgatga tcgggtagtc attggtattg
     1921 gcgagcgtca gcgaacgttc ctgctgtcct tcttcaaaga tcagcctggt gcgttcgacc
     1981 atgatgcccg cgctcgcctg aagacaatgc attcccgcca gacagagcaa aaccaccttg
     2041 accagcaagg catgtcggcg ccttgtcaag aaaaatgggg gcaatgtttt cattggactt
     2101 tcacgatgac ataagcaatc gcatagactc tgcccggcgt gatcacctct cctggaagtt
     2161 tttccagcga agcagtaatc acacgtgtgt aattgaccgt ttttccggaa cccgagggga
     2221 cacccccact gaaaacggga taccacccgg caccagcgcc agtaccagga tcggcatcac
     2281 ccggccatcc aagaaaattc atcggttgtc cactatcggc atcgcggatg cggatgccaa
     2341 ccccggtagc gatcgccgga tcgacaccat agttggtcga cagcagatga gtgacgccgc
     2401 ccatgctgtc gaccagccca agctccatcg ccttggcata agctgcgaca gatacctgta
     2461 tgcccatggc ggtctgattt cgtcccgtac ccgacttgac attacggtcg cattcaagct
     2521 gaatggagaa gtcagcttcg cgtatctcac ctcgttgaaa ctcttgaatc gtaatgggcc
     2581 agaacgtcac ttcgggcgtt gtattgcgta cgacacacgt cggttcatag ctgaccgacg
     2641 ccgcattccg catcccgaaa gcgacaccat ttctatctcc ccagaaacga taattatacg
     2701 catggtcact gccttctgga tcggcaggat atcccggccc actaaaagca atataggcat
     2761 tgggctgcgt gcagctataa ttccaggggg aggagcctcg gccatttgcc atgttgttgc
     2821 cgcaataatt gcttacacca cccagttgta cggtcgatac ccgagccagt tcagcctgta
     2881 ccggactcaa atgttttgcc ctgattagga tcttgttgtc cttcacatcg taattcgtga
     2941 gtttcgtata ctgccagtga cgtgtgaaca ccttgcctga tctgacatgt gtaatcttga
     3001 tggccgcaaa agaaaaccag gtttggtaat agtctggcag gccatccttg gcgccgatgt
     3061 cccagtaacc gccgacccgg tcatcgccat tggtggaaaa tacctcgtag agttgatcct
     3121 tatccgccag atcgcacctc cacagcacgg tttcgtcatt gatgccgaca gcattgcctg
     3181 ccgttgcaac cgtcgacccc agtatggtac ccacaggctg caatatggtg ctggtgatat
     3241 tgatccggcc gaaccgtaac tgtgacgagc ccgcatccag cacgggagta tagggagagt
     3301 tgctacgctc acaccttgcc tgcgcggcaa cgcaagacag caggccgaca agcatgaata
     3361 ccatccgggt caggaataag ctggcacttt ctttcattgg cattccctat tcattttctc
     3421 tcaacgactc tcacagacgc ccttcagctt caaaagcggc tgctgctggt cctcttcctg
     3481 taaatcataa ggcagctcgc attgttcctc tgccgcttcg ccccagcgca agatcagttt
     3541 gccgtgcata ttcccggtac gcacataggc ctgactgccc tgccccacca tgccgataat
     3601 gacgccctgt tcatccagga cgtccgcccc tatcggcacc ggattgccat cttcgcgcag
     3661 cgccttgatc aacagggcat tgccgctgcg cgtcttgaac tggaccttga tcgcagcgcc
     3721 cgcataagga acgacctggc gctgcccgtc attgagttcc accttgctgt tcatgccttc
     3781 cggcatcaag ccgacctggt tacgctgata aggcaccagc gacggcatca aggcataacc
     3841 attgctgtca atcgcagtac tctggccgtt gaacactttc gccccggttg ccccctttgc
     3901 ttcgatcagc gcgaacgtat cgctcagata agggccaaac gtgataccgc ccgaatggat
     3961 ggcgacagct ccgcgcgcgc tggccgcagc ctgccaatag cctgggccgt gtgaggcatt
     4021 gatgcccatg ctgacgttgg caaactgctt gtgtaaattg ccgccgatgg cattctggcc
     4081 atcatgggca tagttcacat caaggccgta gttcatgctc tggccttgtc ccagagtacc
     4141 cgatatggat gcctgatagg tattctcgcc gttagccctg ttcacagagg aactgaagaa
     4201 tggccggctg tcgctacccc tcggccccaa cgggaaaaat actgtcacga cgacggacga
     4261 ctcccggttt tcggtgcttt ccaaaaaacc ggcgccagcg aactgaccgc tgaagacccg
     4321 ctgccgctga aatgccaggc tcaaactgac gtcgttctcg aataccacgg tgtatccggc
     4381 ctgcagttgc gttacgcgcc tgctgctgcc gtaataatcc tgcgtcagtc cggagagata
     4441 gagcgatcct gcctttccca ggcgctggtt cagtgaaatg tcaaagcgcg aacgctgcct
     4501 gtaagaagac gacagcccga taccgctgtc cagctggtat cggcgctcgc tttgcacatc
     4561 ccccagttcg cgataattct gggtcgaata acggtaactg gcaagcgaaa acgtcgtatc
     4621 agtacgctga aaggtcttgc tgtatgtgac acctgccatc cagccgttcg ctctttttcc
     4681 ctgcgccagc attgcgtgcg aataggccgt gttgagtccc aatgcgccaa gcacactgac
     4741 atgcgtcacc cccatggcta tagcctgata accgttcgat atgcgcagcc cgccattggg
     4801 cgtaagcgaa ttgctcaagc catattgata ggtcacatcc gcgaacagat ccttcgcagc
     4861 agcgtcgcgc gtcctgccca gcgtaaaact gtaacgggaa gcgcccggac gcatcgactc
     4921 cggcaccgcc gcaaagggaa cgctgaactg tgaacgcgta ccgtccgctt ccagtacggt
     4981 gacatccaga tcaccgttat agctagtggg atacagatcg ctgatttcga atggaccagg
     5041 cgccaccgtg gtccgataga tttcctgacc attctgatgc acgctgacct gcgcattagt
     5101 gcgcgccacg cctctgatca ccggcgcata cccacgctgc gattccggca acatccgatc
     5161 gtccaggcca gctcgatacc ctgataggcc acaccagaaa aaaatactcc cgacgtgtag
     5221 ttttcaccaa acgagagttc gcccctgatg cctggcaacg cacgcttgac ataggttctg
     5281 actgaattcc actgatatcc gttggacggg tcataactca tcgacccctg ctggcgcaaa
     5341 cgccacaatc ccaggttcaa tccactgttg gtcgaaacat aggtcgaatt ctgggagctc
     5401 accgtcgtgg acgcgtaata ctgactggca ttgtaattga cgaagccgat ggcatccccg
     5461 ctctcaaagc gcttaggatc gacatagccg cgcggcctgg tgttcatggc aatctgggga
     5521 atcgagagat ccagcctgag gtgggaaaaa tcgaagcgtt cggtcgcacc tgcaacgaca
     5581 ctcgagataa actggcaatc agtatttttc tccgctgcag ccatgatatc ctccgcaatc
     5641 ccgatgcccg tcagcacatc tccagataaa caggcgcgcg tagtgccgtc gacatcaccg
     5701 aaacgaatat tctggcgatc atagagacgt ccgttgacat acaggtcgac gtcataggtt
     5761 cctggctcga tggtgtcgct gcgattaaat cgcgacaagc ttcccctgtt ggccagcgat
     5821 cccaatgcca acccctcgat aaacaggtaa gcacctttag catcaactgg ctccgcctcg
     5881 tttttccctg cacccaccgc tgcctgggct tgtacgacaa acccgtacaa cgatagcagc
     5941 aaccatggca gcgatcgcaa cacaaggcac gatgccactc gccggccacg ggaaaagccg
     6001 aaacaagcac cgttacttga aacgatactc gtcttcaatt tcgccaccgt aatcattaat
     6061 caacctcacc ttgaggcggg aagcgcttac ctcgccagtc atcgtcttca caggccagac
     6121 gacctcaccc ttcggaggaa tcaacactgc ctcagcaacc ggcacatcgc ctttgtcgga
     6181 ggcgataatg acgctctgaa gcaccgcata gtaaccactg gaattacgcc cgacgacacg
     6241 aaccacgcca tcggcgcgac ttgcttcaaa agacagactt ttgcgcatgg tggacatgct
     6301 cgactcattg gaaagagcag tcgggcgata aaaaattttc attcggctgc gcaccgtcag
     6361 caccagcttg ttggcctcca tatcggatgc ttttattgcc ggaacctgca ggaaattaag
     6421 aaaaaacagg gactcgcgat cctgcggcag cgtttcgcca acatatgtca gtcgcaccat
     6481 ctgccctgcc tgcggctcca tccgaaagac cggcggatga gcgacaaaag gaggatcctc
     6541 cgattcggaa cctgaagacc catttccctg gtccacccag atctggacca gattcgggta
     6601 attatcctga ttcgtgaagt tcaatacccg ctctttgagg gaggctggat aaaccacgcg
     6661 cgtacccacc atgatgacgc tggcatgcgc cggggaagcc gacacaagtg cagcacagaa
     6721 catgagcgca tatttcaaga taattttttt catattcgac atcgacacac cttcttcttg
     6781 cgattcggcg cgaacccaaa caaaagatcg cagaagcgat acacgaccgc tattgctgat
     6841 atcactgcag ccatgcaaat accagtatca ggcaatttaa tttctgatac aaatcataga
     6901 cacctgccta ccctgatacc ttgcaaatgg gaaactatgg cagacgattg tccggagtca
     6961 ggagatgacc ggccataccg ccggcatgat agaaattgca atcatagtct ctgcactaca
     7021 agagactctg tgaacaaggt tgagaacgaa cgaataccga catcaaccca actcaccgca
     7081 aatccaaaga gagaagcaaa aaaacagaag agtatgccga catcaagtcg acatactcat
     7141 actcggctat ccaagatttt cagatactca cacgtgctac ccagagaacc agcaaaaaga
     7201 agcacagatc caaaaaatgg caccgattca cgccacgatt acaaataatc cagcgcgtat
     7261 tgcacagctc ccatgaccga gccggcacca acagcagcac cctcagcaat ataacgcacg
     7321 gcgaaaatgt ggctagcggc agtctcgccc ctaccaagct tgagtccctc gaccgaggtc
     7381 gccgtgccat taaccagttt tattgggcta ccacctggct gatccagcaa ttgcaacgcg
     7441 acattgcttg ccgtacccag attggggatg ttgccgccat ccgtcgcccc tgaagcaaca
     7501 gcgaacgctg tcttgatcga catgacgtcc gtaacaggtg ccttacatcc cgtgacggtc
     7561 aaggtaaaag gcctttcacc cgccacagct ccggccgccg caagtgaact cgtcgctact
     7621 gtcggaagaa ccaccaacgg acccatagca ccctcaacca taacatgaca ggtttgatcg
     7681 ctaacctgcc cctggaatct gattgtattg cctgcagcga acacgaggga cggcattgca
     7741 gtcaacagta atactacttt tttcattatt aaatttcctc tatttaaaaa ttaaaacctg
     7801 gttcagaatt taagttgcca tatagcacag tatcgcaagc aatacatcta tgaattatag
     7861 attgacaacg atatccttag atataagaat caccttaata tgccatggga tattaacaca
     7921 aaaataaaac gcgccagctt aacagatttt cagagaagca tcagttatca agccaataac
     7981 aatacatcgg ctggaaaaac agttctacac ttgtgcattt ctttggcaca agccagatca
     8041 gaaaaaagcc gttattttaa actataaatt atttgacgcg ctgtttttcc aacttacgtg
     8101 ccagtgtgcg gcgatgcatg ccaaggcggc gtgctgcttc agagatgttg aaatcagtat
     8161 ctgccaagac ttcatggata cactcccact ccatcgtctt gatcgaggtt gaacgcgtcg
     8221 tcaactcgat ttcggtgctg ccggcgacat ggccaaaagc gatttcgata tcgtcggtat
     8281 tggatggctt agccagatac tggcaagcac ccagtttgat cgcctcgacc gctgtgccga
     8341 tgctggcgaa tccagtcaac accacgatca gcatggcttc atcatgctga tgtagcatct
     8401 gcacgcaggc cagtcccgaa gtattgccgt tgagtttgag gtcaaccacg gcataaccag
     8461 ggttgtgctg tgccagcaag gcggcagctt cactctgagt agtcgccagc aacacgacat
     8521 agccgcgccg ctcgaacgaa cggctcaaag tgcgggcaaa agccatatca tcttcaatga
     8581 tcaacaacag gcgctctgtc gacatattgc cgattcccct tcccctgttc cgctgtgttt
     8641 ttttgcgcat cgacttcgaa actgatagat gaaagtcgca gtgtaaggca taccgtagca
     8701 ccgacgacac gaccgttttc ttcacgatta tgcgctgtaa cagtcccgcc gagtttacgg
     8761 gcggcattca ttacaaaaaa cagccccaat ccgccaccta tcttgccttt ggtcgactgg
     8821 taaggctggc ccagctgtgc cagcatacct gtgacgaaac ccgggccaat gtcgtccacc
     8881 gtcagacgca gcataccgcc ttcgcgcact acagtcaggc gcagccagta tggcgatacc
     8941 tccagtgcat tgtcgagcac attgtaaatc atttgcttga gtgtcgaatc aaaaatgacc
     9001 gacatatccc gctcgatgcg attgtcgtat tcgaaagaaa cgataggccg cgtggtacga
     9061 aattcttcca tgatgtcact caggaaggtg ttgagcgagg ttcggagcga cgatgcgcca
     9121 cgccactcgc cagctgacag caacacgcca ctgacgatgg ctttacagcg ttgcaattga
     9181 atctgcatct cgtcgatttc ttccagcagt acggaattgc tgctgaactc gggcatgtgt
     9241 cgccaatcac caagaatcac tgacaacatt gctaacggcg tgccgagttc atgtgcggct
     9301 ccagaagcca gcaaacccat acgtacaata tggtcttctt ctacagcgcg ctggcgcagt
     9361 gtggccagtt gggcatcggc gatgcgacgg ttgccgctga tgcgcgtgat gacagtcacc
     9421 agtaatatcg aaaccatgat aaagcaaagt agcatgcctt cgacgtacct actggataat
     9481 aagcccgcgc tacgatccaa tgccaaggaa agcggtatga aaaactgcga tagcccggca
     9541 aaacacagac tggtggtgca gacaatactc caggtcgacc agacttcgag caacaccgcg
     9601 ctaatgatga tcagtaacaa atataaaaaa acaaacggat tggtggtgcc gccgctgaaa
     9661 taaagctgcg cagtcagcgt ggcgacatcg accagcaagg ccagaaacaa ctcgcaattg
     9721 gtgacgaaaa gacgttcgtg ccaacgcata tagctgccaa tgttgaaggc gatcaggcaa
     9781 gccagtactc ccaacatatg cggcaacggc agttggatgt caaaactgaa aaccgctacg
     9841 atgatggtgc cagcctggcc caacacggca acccagcgta actggatcag ttgcaacata
     9901 ctcttgtggc cgacggtgcg atcaggcatg ccggctgggt tttctgagcc tgaggagaaa
     9961 acattgccca tggcggcgtt gttatgctaa tgggttaaaa ccttgaaatc gagaatattc
    10021 ggagaattat tagccttgcc tgccattatc gctttcctgg ctaaatctga ctgcgctggc
    10081 gattttcgtc gcgcatgacc caataaccag catagacagt catcaaggcc aatccatacc
    10141 aggtgagaat ataaaccaga tggttgttat gaaaaacaat cactgtcaac ccgccaaccg
    10201 gattgccagc agcggcacgc atctcttccg tcatagcaac gtccgctgca gctgtcacgt
    10261 cggcatcgac aaaatacggc gccaccttgc caacaagctt gtgtgccgcc gcaatggcct
    10321 gtacgtcacg cgaaaaccag cggccggtgg ccggatcatt attgcgcaag aagcctccgc
    10381 tcggttcgct gatacgcagc agaccggtga gcctagcagg agcatcactg gctgcttgcc
    10441 ggatatcgtg cgcgtcggca gcaatgtagc cgcgattgat cagcgtcacc atcccatcgg
    10501 gaccgcgcaa cggcgtcaac aaccagaaac cactacccag acgagtaacg gcccgcacct
    10561 ttaccgtttg tgcatataaa aacgtgccgc tgaggcggac atggagatat tcgtaagcat
    10621 cggctgaagc tgatgccgag atgtgagacc actcatccgg tcccggcgcc gcgaccggat
    10681 cggcat
//
LOCUS       DASRDH010000012         2317 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_220977,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000012 DASRDH010000000
VERSION     DASRDH010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220977"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..843)
                     /gene="ligA"
                     /locus_tag="VFA14_00305"
     CDS             complement(<1..843)
                     /gene="ligA"
                     /locus_tag="VFA14_00305"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451051.1"
                     /note="this protein catalyzes the formation of
                     phosphodiester linkages between 5'-phosphoryl and
                     3'-hydroxyl groups in double-stranded DNA using NAD as a
                     coenzyme and as the energy source for the reaction;
                     essential for DNA replication and repair of damaged DNA;
                     similar to ligase LigB; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HZS79865.1"
                     /translation="MFPDAFSDSAPPDDWRARAAWLTEQLNRHNHDYFVLDDPSISDA
                     QYDLLFRELQALELAHPELTAPDSPTQRIGSAPLSQFEQVRHAVPMLSLNNGFDDEDI
                     IAFGRRIEEALLGEQSPSIPHVHFRRPLQRIEEALLGEQYSTADIAYATELKFDGLAI
                     SLRYENGVLAGATTRGDGATGENVTANIRTVRSIPLRLHGDNPPQVLEVRGEVLMYKN
                     DFARLNQRQRDAGLKEFANPRNAAAGSLRQLDPRITAQRTLRFFAYGIGMLEGAVMPP
                     SHSAL"
     gene            complement(865..1995)
                     /locus_tag="VFA14_00310"
     CDS             complement(865..1995)
                     /locus_tag="VFA14_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA C-terminal
                     FtsZ-binding domain-containing protein"
                     /protein_id="HZS79866.1"
                     /translation="MIDLQTSLIVIGGMIVAGVISYNKWQERKTRQTVERAFSSSHDD
                     VLMNADAQSHFLATTSPTPAYQTLAEGGIATLPPSMPGERLEPELYERLEPAPHPPLH
                     LPFPESGPDESDLPVDKSINCLIPIALERPVRGEKILTGVQNLCHAGDKSVRFVGRTQ
                     SDVWEAIGYGGVYTALLAGVQLANRNSALNELEYSDLIMRLREFTDAIGAEPEIPDMI
                     DVMKTAQALHQFIMDHDVQLGVNVRSNGAPWHVDTLLSALTRQGFERRADGRLVMPDG
                     VGETLFSLSTNTIGAVETTNRLTLLLDVPRVAPSRDGYGAMIACARSLAMRLDGTVVD
                     DSGQLLSDAALSDIAEQVSAFYSAMESAQIPAGSKRAACLFS"
     gene            complement(2063..>2317)
                     /locus_tag="VFA14_00315"
     CDS             complement(2063..>2317)
                     /locus_tag="VFA14_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006462554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS79867.1"
                     /translation="FQLNPAPFCLLDEVDAPLDDANTGRFCDMVKRMSSNTQFLFISH
                     NKIAMEMAAQLIGVTMQEQGVSRIVAVDMEAATTPAFQAA"
BASE COUNT          435 a          669 c          736 g          477 t
ORIGIN      
        1 cagggccgaa tgtgatggcg gcatgacggc gccttccagc atgccgatgc cataggcgaa
       61 aaaacgcagc gtgcgttgcg ccgtgatcct gggatcgagt tgacgcaagc tgcctgccgc
      121 tgcattgcgc ggattggcga actctttcag accggcgtcg cgctgacgct gattgagtct
      181 ggcgaagtca ttcttgtaca tcagcacttc gccgcgcact tccagtacct gtggcgggtt
      241 gtcgccatgc agacgcagcg gaatggagcg taccgtgcgg atattggccg tgacgttttc
      301 tccggtggcg ccgtcgccgc gcgttgtggc gcctgccagc acgccgtttt cgtaacgcag
      361 gctgatggcc aggccatcga acttcaattc ggtggcatag gcgatgtctg ctgtggaata
      421 ctgttctcct agtaaagctt cctcgatgcg ttgcaacgga cggcggaaat gaacatgagg
      481 gatagaagga gactgctctc ccagcaaggc ttcctcgatg cgtctgccga aagcgatgat
      541 gtcctcatcg tcgaatccat tgttcagcga cagcatggga acggcgtggc gcacctgctc
      601 gaactgcgac agcggcgcgc tgccgatccg ttgggtcggc gagtccggcg ccgtcagttc
      661 cggatgcgcc agttccagcg cctgtagttc gcggaacagc aggtcgtact gcgcatcgga
      721 aatgctggga tcgtcgagaa cgaagtagtc gtggttatgc cggttcagct gttccgtgag
      781 ccaggccgcg cgcgcgcgcc agtcgtccgg cggtgcggaa tctgaaaatg catccggaaa
      841 catggtgaat gcgttggcag gggtttagct gaacaggcag gcggcgcgtt tcgagccggc
      901 cgggatttgc gccgattcca tggcgctata gaaggcgctg acctgctcgg cgatatcgct
      961 cagcgccgca tcggacagca gctgaccgct atcgtcaacc acggtgccgt ccagacgcat
     1021 ggccagagaa cgcgcgcagg cgatcatggc gccgtagcca tcgcgtgacg gcgcaacccg
     1081 cggcacatcc agcagcagcg tcaagcgatt ggtggtttcc accgcaccga tggtgttggt
     1141 cgatagcgag aacagcgttt caccgacgcc atccggcatc accagccgac cgtcggcgcg
     1201 gcgctcgaag ccttgacggg tcagcgcgga cagcaaggtg tcgacatgcc acggcgcgcc
     1261 gttggaacgc acgttgacgc ccagttgcac atcatggtcc atgatgaact gatgcagcgc
     1321 ctgcgcggtc ttcattacgt cgatcatgtc ggggatctcc ggttcggcgc cgatggcatc
     1381 ggtgaattcg cgcagacgca tgatcagatc ggaatattcc agttcattca aggcactgtt
     1441 gcgattggcc aattgcacgc cggccagcag cgccgtatac acgccgccgt aaccgatcgc
     1501 ttcccagacg tcgctctgcg tgcggccgac gaagcgcact gacttgtcgc cggcgtggca
     1561 caggttctgc acgccggtaa gaattttttc cccgcgcacg ggccgttcca gcgcaatggg
     1621 gatgaggcag ttgatcgact tgtcgaccgg caagtcggat tcgtccggcc cgctttccgg
     1681 gaagggtaga tggagcggcg ggtggggcgc tggttccagg cgttcataca gttctggttc
     1741 gagccgttct ccaggcatgg agggcggcag cgtagcgatg ccgccctctg ccagcgtctg
     1801 ataggctggc gtgggcgatg tcgtcgccaa aaaatgcgac tgggcatcgg cgttcatcag
     1861 cacatcgtcg tgactcgacg aaaacgcgcg ttcgacggtt tggcgggttt tgcgttcttg
     1921 ccacttgttg taggaaatga cgccggcgac gatcatgccg ccgatgacga tcagactggt
     1981 ttggagatct atcatgctga atgcaccctg aaattttccc tgaaaatgcg gctcgccacg
     2041 atgcggccca ttactgtttt tgtcaagctg cctgaaaagc aggggtcgtc gccgcttcca
     2101 tatccaccgc tacgatacgc gataccccct gttcctgcat ggtcacgcct atcagttgag
     2161 cggccatttc catcgcaatc ttgttatgcg agatgaacag gaattgggtg ttcgacgaca
     2221 tgcgcttgac catgtcgcag aaacgcccgg tattggcgtc gtccagcggc gcatcgacct
     2281 cgtcgagcag gcagaacggc gccgggttga gttggaa
//
LOCUS       DASRDH010000013         3481 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_560492,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000013 DASRDH010000000
VERSION     DASRDH010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 3481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3481
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560492"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1258)
                     /gene="lysS"
                     /locus_tag="VFA14_00320"
     CDS             complement(<1..1258)
                     /gene="lysS"
                     /locus_tag="VFA14_00320"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HZS79868.1"
                     /translation="MTAQNDKATQQAALPTDENAIIAERRARLGAIRTQSVAFPNDFR
                     PKHQAADLHARYDALDNDALEADPVKVSIAGRMMLKRVMGKASFATLQDASGARADGR
                     MQLYISNDVTGAAAHDAFRHYDLGDILGVEGTLFKTRTGELSVKVSDLRLITKSLRPL
                     PDKFHGLSDQETRYRQRYVDLIMSEDTRRTFKARTAAMSSIRRFMEKNDFMEVETPML
                     HSIPGGAVARPFITHHNALDMQMFLRIAPELYLKRLLVGGFERVFEINRNFRNEGVSP
                     RHNPEFTMMEFYAAYVDYQWLMSFTEQVIRQAAIDAHGTATLTYQGRALDLSKPFQRL
                     TITSAIRQYAPEYTDAQLQDIDFLRTTLKKFGIKTEQAPLRHAGIGALQLALFEETAE
                     TQLWEPTYIIDYPVEVSPLARASDAAP"
     gene            complement(1305..2409)
                     /gene="prfB"
                     /locus_tag="VFA14_00325"
     CDS             complement(join(1305..2327,2329..2409))
                     /gene="prfB"
                     /locus_tag="VFA14_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079118.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HZS79869.1"
                     /translation="MEAERLNSLHSLLDDLTARAAELRRYLDFDAKSEKLEQVNGELE
                     DPAVWNDPKRAQDLGKEKKFLENIVRTLIRIDGDLDDARDLFAMAREEKDEDTLIAIE
                     ADAATLKGLVEGMEFRRMFSHPMDPNNCFIDIQAGAGGTEAQDWASMLLRQYLRYCER
                     KGFRAEILEQSDGEVAGIKTATIKVEGEYAYGFLRTETGVHRLVRKSPFDSANGRHTS
                     FSSLFVYPEVDDSIDIEINPADVRVDTYRASGAGGQHINKTDSAVRLTHAPTGIVVQC
                     QNDRSQHRNRAEAWEMLKAKLFDLELRKRMSEQQKLEDAKTDVGWGHQIRSYVLDQSR
                     IKDLRTNFETGNTRAVLDGELDGFIAASLKQGV"
     gene            2546..>3481
                     /locus_tag="VFA14_00330"
     CDS             2546..>3481
                     /locus_tag="VFA14_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007879155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase PhoX"
                     /protein_id="HZS79870.1"
                     /translation="MKSARTNTEIQSRRRDLLKAFAGAPLLPLTLTVSGLLPAYATVS
                     SRKGFTAATFSHMPAPSLENPAAMAATTVQSYLEVIFDDGARQSYALAYQPFFMTGDM
                     VPDGKGGRILAGGCYDIDNQPIIDHSLPGKERHFFSNCPDGSSLLTVKGAQVVGVKGN
                     TVFAVVQFEHTTRDQQQADMERRLPAPVAVLTLDQDPQTGQLTLVKYHNVDTSPVNGL
                     WVTCGASLSPWNTHLSSEEYEPDAAAIADDARFQAFSRNLFGDAAKANPYHYGHLPEI
                     TVHADGSGSVVKHYCLGRISHELVQVMPDRRTVLMG"
BASE COUNT          647 a          987 c         1065 g          782 t
ORIGIN      
        1 cgggcgcggc atcggaagcg cgcgccagcg gcgaaacttc gaccggataa tcgatgatgt
       61 aggttggttc ccacaattgc gtttcggcgg tttcttcaaa cagtgcgagc tgcaatgcgc
      121 cgatgccggc atggcgcagc ggcgcctgtt cagtcttgat gccgaacttt ttgagcgtcg
      181 tgcgcaagaa gtcgatatcc tgcaattgcg cgtcggtata ttccggtgca tactgcctga
      241 tcgcgctggt gatggtcaga cgctggaatg gcttcgacag atccagtgcg cggccctgat
      301 aggtcagcgt cgccgtgccg tgcgcgtcaa tggcggcctg gcggatcacc tgttcggtga
      361 aactcatcaa ccattgatag tcgacatagg ctgcatagaa ttccatcatc gtgaattcag
      421 gattatggcg cggcgaaacg ccttcgttgc ggaagttgcg gttgatttcg aacacgcgtt
      481 cgaagccgcc gacgagcagg cgcttcaagt agagttccgg cgcgatgcgc aggaacatct
      541 gcatgtccag cgcattgtga tgtgtgataa aaggcctggc cacggcgccg ccagggatgc
      601 tgtgcagcat cggcgtctcg acttccatga agtcattttt ttccatgaag cggcgaatcg
      661 atgacatcgc ggcggtacgc gccttgaagg tgcggcgcgt gtcttcactc atgatcagat
      721 cgacgtagcg ctgacggtat ctggtttcct ggtctgacag accgtggaat ttgtccggca
      781 gcggacgcag cgacttggtg atcaggcgca ggtcgctgac cttgaccgac agttcgccgg
      841 tcctggtctt gaacagcgtg ccttcgacgc cgagaatatc gcccaggtcg taatgcctga
      901 aggcgtcgtg cgcggctgcg ccggttacgt cgttgctgat gtagagctgc atgcggccat
      961 cggcgcgcgc gccggaagca tcctgcagtg tggcgaacga ggctttgccc atcacacgct
     1021 tcaacatcat gcggccggca atcgagactt tgactggatc ggcttccaac gcgtcattgt
     1081 cgagcgcgtc gtatcgcgca tgcagatcag cggcctggtg tttcgggcgg aagtcgttcg
     1141 ggaaggcgac gctttgtgtg cggatcgcgc cgagtctggc gcggcgttcg gcgatgatgg
     1201 cgttttcatc tgtaggcagc gctgcctgtt gcgtggcctt gtcgttctgt gcggtcatga
     1261 tgtcgggaat gcgtgagtaa aaacgggagc ttcaggtttg ccgatcagac gccttgcttc
     1321 agcgaggcgg cgatgaagcc gtccaactcg ccgtccagca cggccctggt gttgccggtt
     1381 tcgaaattgg tgcgcaagtc cttgatgcgt gactgatcca gcacataaga gcgaatctgg
     1441 tggccccagc cgacgtcggt cttggcgtct tccagctttt gctgctcgct catgcgtttg
     1501 cgcagttcca gatcgaacag tttcgccttc agcatttccc acgcttcagc gcggttgcga
     1561 tgctgactgc gatcgttctg gcattgcacg acgatgccgg tcggcgcatg cgtcaggcgc
     1621 acggcggaat cggttttatt gatgtgctgg ccaccggcgc cggaggcgcg ataggtatcc
     1681 acgcgcacat cggccggatt gatttcgata tcgatcgaat catcgacttc cgggtatacg
     1741 aataggctgg agaacgaggt atgacggcca ttggcggagt cgaacggcga cttgcgcacc
     1801 aggcgatgca cgccggtttc ggtgcgcaga aaaccatagg cgtattcgcc ttcgaccttg
     1861 atggtagcgg tcttgatgcc ggcgacttca ccatcggact gttccaggat ttccgccctg
     1921 aagcccttgc gttcgcagta gcgcagatac tggcgcagca gcatggacgc ccagtcctgc
     1981 gcctcggtgc cgccggcgcc ggcctggata tcgatgaagc agttgttcgg atccatggga
     2041 tgactgaaca tgcggcgaaa ttccatgcct tcgaccagcc ccttcagggt ggccgcgtcg
     2101 gcttcgatgg cgatgagcgt gtcttcatcc ttttcttcgc gcgccatcgc gaacagatcg
     2161 cgcgcatcgt cgagatcgcc gtcgatcctg atcagcgtac ggacgatgtt ttcgaggaat
     2221 ttcttctctt tgccgagatc ttgcgcgcgt ttgggatcgt tccagactgc gggatcttcc
     2281 agttcgccgt tgacctgctc gagtttctcc gacttcgcat cgaagtcaaa gatacctccg
     2341 tagttccgcc gcgcgcgccg tcaggtcgtc cagcagggag tgaagggaat tgagacgttc
     2401 tgcttccatg atgatttctt taacgatcaa accatacatt atacaagact gaagatgctg
     2461 tttctttgcc gcaaagcggt catgttgtac gatgagcaat cgtatttgtc agctcgttat
     2521 cagaccgtta ttaaaagggg acgtcatgaa atccgctaga acgaacactg aaattcagtc
     2581 caggcgccgc gatctgctca aggcattcgc cggtgcgccg ctgttgccgc tgactctgac
     2641 cgtgtccggt ctgctgcctg cttacgctac cgtttccagc cgcaagggtt ttactgcggc
     2701 gaccttcagt cacatgccgg cgccgtcgct ggagaacccg gcggcgatgg ctgccacgac
     2761 cgtgcaatcg tatctggagg tgatctttga cgacggcgcg aggcaaagct atgcgctggc
     2821 ctaccagccg ttcttcatga ccggcgacat ggtgccggac ggcaagggcg gcaggatact
     2881 ggccggcggc tgctatgaca tcgacaacca gcccatcatc gatcattcgc tgccgggcaa
     2941 ggagcgtcac tttttttcga attgcccgga cggctcttcc ttactgaccg tgaaaggcgc
     3001 gcaagtcgtc ggcgtcaagg gcaataccgt gtttgccgtg gtgcagttcg aacacaccac
     3061 gcgcgaccag cagcaggccg atatggagcg ccgcctgccg gcgccggtcg ccgtgttgac
     3121 gctggatcag gatccgcaaa ccggccagct cacgctggtg aagtaccaca atgtcgatac
     3181 ttctccagtc aatggattat gggttacctg cggcgccagt ctgtcgccgt ggaataccca
     3241 tctgtcgagc gaagagtacg agccggatgc tgccgccatc gcggacgacg cgagatttca
     3301 ggcgttcagc aggaacctgt tcggcgacgc tgccaaggcc aacccctatc attacggcca
     3361 tcttcctgaa atcaccgtcc acgccgatgg cagcggcagc gtggtcaagc attactgcct
     3421 gggccgcatt tcgcatgaac tggtgcaggt catgccggac cgcaggaccg tgctgatggg
     3481 c
//
LOCUS       DASRDH010000014         4210 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_677223,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000014 DASRDH010000000
VERSION     DASRDH010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 4210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4210
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_677223"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1355)
                     /locus_tag="VFA14_00335"
     CDS             complement(<1..1355)
                     /locus_tag="VFA14_00335"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HZS79871.1"
                     /translation="MSAEFFTDPRAAIDRVIDIYERNTAVLRSAFRQFAKGVVLTERV
                     RMCYPFVRITTDKVTHADTRQSFGFVAGSGTYQTTLTRPALFRNYLLSQFELLLLNHQ
                     EPLEIGVSDLPIPVHFAFAEGIHVEGDLDPDHLRLLPDVFDLPDLAGMDDRIANGTYE
                     ADNGGWRKGVHPLALFTAPRIDYSLHRLHHYTATAPHQFQRYVLFTNYAFYVDEFVRM
                     GRELMQLTDDPVLRAYRQQYTAFVEPGNVTTPNANVGSMVGSGTPPARQPQMPAYHLQ
                     RADGTGITLVNIGIGPSNAKTITDHVAVLRPHGWIMLGHCAGLSSSQRMGDYVLAHAY
                     VRDDHVLDDDLPLWVPIPALAEVQLALQGAVADITQLEGYELKRIMRTGTVASVSDRN
                     WELSDYRMPLLRFSQSRAIALDMESATVAANGFRFRVPYGTLLCVSDKPLHGEIKLPG
                     MA"
     gene            1480..2391
                     /locus_tag="VFA14_00340"
     CDS             1480..2391
                     /locus_tag="VFA14_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS79872.1"
                     /translation="MLLCNLAFPWWKANAKTMALISRGSIAYDFHENTFKGAFMYKRY
                     ALSLSVLMLASAASAQEIKPVYEIGAEIYHEDYKSTLKSGRTFVKEKATMYGINGTIG
                     AIRGQHGLKLTGRYAQGKSDHTGSLSGVFGTLKGDGLDRRSFEIRGIYEYTMPFPDFD
                     LVPSVGLGYRYLLNRLDQMPLGDERKDGYIFLPLGLEATFRPSDKLHITPKLGYNYLI
                     EGRQETDYRVERAASKKKQKSGNGFEISAAFAYTLENNSVVRITPFYRYWDIDHSETV
                     RKPLSNELIYDPKNKTDEFGVNVTYAF"
     gene            2661..>4210
                     /gene="dnaX"
                     /locus_tag="VFA14_00345"
     CDS             2661..>4210
                     /gene="dnaX"
                     /locus_tag="VFA14_00345"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HZS79873.1"
                     /translation="MSYQVLARQYRPRNFDTLVGQEHVVRALTHALDQQRLHHAYLFT
                     GTRGVGKTTLSRILAKSLNCQGADGNGSITSQPCSVCEACVAIDAGRFVDYIEMDAAS
                     NRGVDEMAQLLEQAVYAPSNARFKVYMIDEVHMLTNHAFNSMLKTLEEPPEHVKFILA
                     TTDPQKIPVTVLSRCLQFNLKQMPPGHIVNHLDKILSQENIAFEASALRLLAQGACGS
                     MRDALSLTDQAIVYAAGTVTLDAVQGMLGTLDRSCLVRILDALAMQDGAALLAVADDM
                     ATRSLSYSVALQDLATLLHQLALAQTVPAALAEDAPQREDIVRLASQFTPEEVQLFYQ
                     IAVHGRNELGLAPDEYAGFSMTLLRMLAFRPVTASGVPAAPAEPAVSAPARPNPAIAS
                     LEAARAASGKRVSHPAATSLQTTLLQKMAPVSPAMPGDAPAPAADTPTLALPAQTVQI
                     AAELDWDGDWRQLASDLPLRGMAAQMVRQSELICCARHGNTVYFHIRIPVDTLRAAGN
                     IEKLSTVL"
BASE COUNT          971 a         1232 c         1144 g          863 t
ORIGIN      
        1 gccatgccgg gcagcttgat ctcaccgtgc aaaggtttgt cggatacgca cagcaaggtg
       61 ccgtagggaa cacggaaacg gaagccgttg gccgccaccg tggcgctctc catatccagc
      121 gcaatcgccc ggctctggct aaagcgcagc aaaggcatgc gatagtcact caactcccag
      181 ttacggtcac tcaccgaagc gaccgtaccg gtacgcatga tgcgcttgag ctcatatcct
      241 tccagttgcg tgatgtcagc gacagcaccc tgcaaggcca gctgcacttc tgccaatgcc
      301 ggaatcggta cccataaagg caaatcgtca tccagcacgt gatcgtcgcg cacataggca
      361 tgcgccagca cataatcgcc catgcgctgc gacgacgata atcccgcgca gtgccccagc
      421 atgatccagc cgtgcggacg caggacagcg acgtgatcgg tgatggtctt ggcgttggat
      481 ggaccgatgc cgatgttgac cagagtgatg ccggtaccat cggcgcgctg cagatgatag
      541 gccggcatct gcggctggcg cgccggtggc gtacccgagc cgaccatgct tcccacattg
      601 gcattcggcg tcgtgacgtt acccggctcg acaaatgccg tatattgctg acgataagcg
      661 cgcagcaccg gatcgtcagt gagctgcatc aattcgcgcc ccatgcgcac gaactcgtcg
      721 acataaaacg cgtagttggt aaacagcaca tagcgctgga actgatgcgg cgctgtcgcc
      781 gtgtaatggt gcaggcgatg cagcgaataa tcgatgcgcg gcgcggtaaa caaggccagc
      841 ggatgaaccc ccttacgcca gccgccattg tcggcttcat acgtgccgtt ggcgatacga
      901 tcgtccatcc cggccagatc gggcaaatcg aatacatccg gcaacagcct caaatgatcg
      961 ggatcgagat ccccctcgac atggatacct tcggcaaaag caaagtggac cgggataggc
     1021 aaatcgctga caccgatttc cagcggctcc tgatgattca gcagcaacag ctcgaactgg
     1081 ctcagcaggt aattcctgaa cagcgccggg cgcgtcagcg tagtctggta agtgccggac
     1141 ccggcgacga aaccgaacga ctggcgtgtg tccgcatgcg tcaccttgtc ggtggtgatg
     1201 cggacaaagg gatagcacat gcgcacgcgc tccgtcagca ctacgccttt ggcaaattgc
     1261 ctgaatgcgc ttctgagaac ggcggtgttg cgctcataga tgtcgatcac gcgatcgatt
     1321 gccgctcgcg ggtcggtaaa aaattcagca gacaatgttt ctctcacgac aaatcaaggc
     1381 ataccgggat gaatgttgcg ctcggaacca caaaaacgat aggactgttg tatttacgta
     1441 ctaacacaaa gccacaatag ttaccaatcc catattttaa tgctactctg caacctggct
     1501 tttccttggt ggaaagccaa tgcaaagaca atggctttga tttcgcgtgg gtccattgcg
     1561 tacgatttcc atgagaacac tttcaaaggg gctttcatgt ataaaagata tgcgcttagt
     1621 ctgtcggtat tgatgctggc atccgctgct tcggcacaag aaatcaagcc agtatacgaa
     1681 ataggggccg agatttatca cgaagattat aagtcaaccc taaagagcgg tcgcactttc
     1741 gtgaaggaaa aagcgacgat gtatggcatc aacggtacta ttggtgcgat acgcggacag
     1801 catggcctga aactgaccgg gcgctatgcc cagggcaaaa gcgaccatac cggcagtctg
     1861 tcgggagtgt tcggtacatt gaagggcgac ggactggatc gcagaagctt cgagatacgc
     1921 ggcatctatg aatacaccat gccgtttccc gatttcgact tggtgccatc agtcggtctc
     1981 ggctaccgct atctgctgaa ccgccttgat cagatgccct taggtgatga acgtaaagat
     2041 ggatacattt ttctgccgct tggcctggaa gcgactttcc gtccctcgga caaactccac
     2101 attaccccaa agcttggcta taactacctg atcgagggca gacaggaaac agactatcgc
     2161 gtggagcgtg ctgcctccaa gaaaaaacag aaaagtggca atggtttcga aatatccgct
     2221 gcgtttgctt atacgctgga aaacaatagc gtggtgcgta ttacaccatt ctatcgttac
     2281 tgggacattg accatagcga aactgttcgc aaaccgctgt caaatgaatt gatttatgat
     2341 ccaaagaaca agacagatga attcggcgtc aatgtgactt acgcattctg attttttccg
     2401 cgtcccattc gtgcgcaacc tgttatcggc ctgcccggtg cctgtcactg gctgcgcaca
     2461 gtccacccct gtcgataagt acggtgacag ggcgttgcct gtcattggtt gcgcatagct
     2521 cgccttgatc ggagcattct ccatgctccg ccgggcagat acagataagc aacgcttccg
     2581 cttcctgcgg gcaaattatc ctgaatccag caaatagaat aaaatgcctg tctgagccaa
     2641 cgccggcatc ctaaggtttt atgtcttatc aagtcctcgc ccgtcaatac cgcccccgca
     2701 acttcgatac gctggtcggg caggaacatg tcgtgcgggc gcttacccac gcgctggacc
     2761 agcagcgcct gcaccatgcc tatctgttta ccggcacacg cggcgtcggc aaaaccacct
     2821 tgtcccgcat tcttgccaaa tcgctgaact gccagggcgc cgacggcaac ggcagcatca
     2881 cctcccagcc ttgcagcgtc tgcgaagcct gcgttgccat cgatgccgga cgctttgtcg
     2941 actacatcga aatggatgcc gcttccaatc gcggcgtcga cgaaatggcg caactgctag
     3001 agcaggcagt ttatgcgccg tccaatgcgc gcttcaaggt ctacatgatc gacgaagtac
     3061 acatgctgac caaccacgcc ttcaattcca tgttgaagac gctggaagaa ccgcctgaac
     3121 atgtcaaatt catcctggcc accaccgacc cgcaaaagat tccggtgacc gtgctgtcgc
     3181 gttgtctgca attcaatctc aaacagatgc cgccgggcca catcgtcaat catctagaca
     3241 agattctcag ccaggaaaac atcgcattcg aagcatccgc gctgcgtctg ctggcacaag
     3301 gcgcgtgcgg ttccatgcgt gacgccttgt cgctgaccga ccaggcgatt gtctatgccg
     3361 ccggcacagt gacgctagat gcggtacagg gcatgctcgg cacactggat cgatcctgtc
     3421 tggtgcgcat actcgatgca ctggcgatgc aggacggcgc ggccttgctg gcggttgccg
     3481 atgacatggc cacacgcagc ctgtcttaca gcgtcgcact gcaagacctc gcgaccctgc
     3541 tgcaccagct tgcgctggcc cagaccgtac cggcagcgtt ggcagaagat gcgccgcaac
     3601 gtgaagacat cgttcgcctg gcatcacaat tcacacccga agaggtgcag ctgttttatc
     3661 agattgccgt gcatggccgc aacgaactag gtttggcgcc ggatgaatac gccggtttca
     3721 gcatgacgct gctgcgtatg ctggcatttc gtccagtcac cgccagcggc gttcccgcag
     3781 cgccggctga accagccgtt tctgctcctg cgcgccccaa tcctgctatc gcgtcgctgg
     3841 aagcggcgcg cgcagcctcc ggaaagaggg tgtcgcaccc tgcggcgaca tcgctgcaaa
     3901 ctacgctatt acaaaagatg gcgccggtat cgcctgccat gcctggcgac gcgccagcgc
     3961 ccgcagcaga tacgccaacc ctagcgctac cggcgcagac cgtgcagatc gccgccgaac
     4021 tggattggga cggcgactgg cggcaactgg catcggattt gccgttgcgc ggcatggcgg
     4081 cgcagatggt gcggcaaagc gaattgatat gctgcgcaag acatggcaat acggtgtact
     4141 tccatatccg cattccggtc gacaccctgc gcgccgcagg aaatatcgag aagctcagca
     4201 ccgttctgag
//
LOCUS       DASRDH010000015         3149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1137328,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000015 DASRDH010000000
VERSION     DASRDH010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 3149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3149
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1137328"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            54..524
                     /gene="rpsE"
                     /locus_tag="VFA14_00350"
     CDS             54..524
                     /gene="rpsE"
                     /locus_tag="VFA14_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HZS79874.1"
                     /translation="MKEKMISINRVTKVVKGGRILGFAALTVVGDGDGRIGMGKGKSK
                     EVPVAVQKAMEEARRKMIKVALKNGTLQHTVTGRHGASSVLMSPAKDGTGVIAGGPMR
                     AIFEVMGVTNVVAKSNGSTNPYNMVRATLDGLSKMSTPAEIAAKRGKSVEDILG"
     gene            535..714
                     /gene="rpmD"
                     /locus_tag="VFA14_00355"
     CDS             535..714
                     /gene="rpmD"
                     /locus_tag="VFA14_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HZS79875.1"
                     /translation="MANIIKVQLVKGLIGIRQDHRATVRGLGLRRINSVSELQDTPSV
                     RGMINKVSYLVKVVS"
     gene            741..1172
                     /gene="rplO"
                     /locus_tag="VFA14_00360"
     CDS             741..1172
                     /gene="rplO"
                     /locus_tag="VFA14_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HZS79876.1"
                     /translation="MELNTIQPAEGARRHGRRVGRGIGSGLGKTAGRGHKGQKSRSGG
                     FHKVGFEGGQMPLQRRLPKRGFKSLATPYKAEVRLSDLEKLPVDEIDILALKQAGLVP
                     ELARFVRIILAGALTRKVTVKGLVATRGAKAAIEAAGGSVA"
     gene            1198..2529
                     /gene="secY"
                     /locus_tag="VFA14_00365"
     CDS             1198..2529
                     /gene="secY"
                     /locus_tag="VFA14_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006464914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HZS79877.1"
                     /translation="MATTPRLAQGAAKGFPWSRLWFLLAALVVYRIGAHIPVPGIDPA
                     QLAQLFKQNQDGILGMFNMFSGGALSRFTIFALGIMPYISASIIMQLLSVISPQLEAL
                     KKEGEAGRRKITQYTRYGTLVLATFQALGIAVALESQAGLVLDPGLAFRLTTVVTLVT
                     GTMFLMWLGEQITERGLGNGISIIIFAGIAAGLPNALGGLFELVRTGSMSALLAILIC
                     LIVALVTFLVVFIERGQRKILVNYAKRQVGNKIYGGQSSHLPLKLNMAGVIPPIFASS
                     VILFPATITSWFTSGDTSNPFIRFLKDLAASMAPGEPIHALLYAIAIVFFCFFYTALV
                     FNSKETADNLKKSGAFVPGIRPGEQTARYIDKILMRLTLVGAMYITLVCLLPEFLITR
                     WKVPFYFGGTSLLIIVVVTMDFMVQVQNYVMSQQYESLLRKANFKGGMTSR"
     gene            2561..2779
                     /gene="infA"
                     /locus_tag="VFA14_00370"
     CDS             2561..2779
                     /gene="infA"
                     /locus_tag="VFA14_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HZS79878.1"
                     /translation="MAKDDVIQMQGEILENLPNATFKVKLENGHVVLGHISGRMRMNY
                     IRILPGDKVTVELTPYDLSRARIVFRTK"
     gene            2808..2921
                     /gene="rpmJ"
                     /locus_tag="VFA14_00375"
     CDS             2808..2921
                     /gene="rpmJ"
                     /locus_tag="VFA14_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HZS79879.1"
                     /translation="MKVLASVKRICRNCKIIKRKGVIRVICTEPRHKQRQG"
     gene            2950..>3149
                     /gene="rpsM"
                     /locus_tag="VFA14_00380"
     CDS             2950..>3149
                     /gene="rpsM"
                     /locus_tag="VFA14_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HZS79880.1"
                     /translation="MARIAGVNIPNHQHIVIGLTAIYGVGRSRAEKICVATGVSTSKK
                     VKDLNDSELEKLRDEIAGFVVEG"
BASE COUNT          731 a          724 c          920 g          774 t
ORIGIN      
        1 agcaacgatc caactcccgc cagtcgcagc agcaggacga ccgcggcgac gggttgaaag
       61 agaagatgat ctcgatcaac cgggtcacca aggtggtgaa gggcggacgg atcctgggct
      121 tcgcggcgct cacggtcgtc ggcgatggcg atggtcgcat cggcatgggc aaaggcaaat
      181 cgaaggaagt gcctgttgcc gtgcagaagg caatggaaga agcccgccgc aaaatgatca
      241 aagtcgcctt gaagaacggt acgctgcagc acactgtgac cggcaggcat ggggcatcat
      301 cggtgttgat gtcaccggct aaggacggta ctggcgtgat cgcaggcggt ccaatgcgcg
      361 cgattttcga agtgatgggc gtaaccaacg tcgttgccaa gtcgaatggt tctaccaatc
      421 cttacaacat ggtacgtgca acgctcgacg gtttgtccaa aatgagcact ccagcagaaa
      481 ttgcagccaa gcgcggcaaa tctgttgaag acatcctggg ctaaggtgaa acagatggca
      541 aacataatca aggtacagct ggtcaaaggc ttgatcggta ttcgccagga ccaccgcgca
      601 acagtgcgcg gtctgggact gcgtcgtatc aattcggtct ccgaattgca ggacacgcca
      661 tcggtacgcg gcatgatcaa taaagtgtcc tatcttgtga aagtggtgtc gtaagcctct
      721 ggcttgtgaa tggagtgaac atggaattaa acacaattca acccgcggaa ggtgcccgac
      781 gccatgggcg tcgggtcggg cgcggcatag gttccggcct gggcaagaca gccggccgtg
      841 gtcacaaggg tcaaaaatcg cgttcgggcg gctttcataa agtcggtttc gaaggtggtc
      901 agatgccctt gcaacgccgt ctgccaaagc gaggtttcaa atcgttggca acgccgtaca
      961 aggctgaagt acgtttgtct gatctggaaa agctgccggt agacgaaatc gatatcctgg
     1021 cgctgaagca ggctggtctc gttcctgagc tggccaggtt tgttcgcatc atcctggcgg
     1081 gcgcgctgac taggaaagta acagtcaaag ggctggttgc taccaggggc gcaaaggctg
     1141 ctatcgaggc tgccggtggt tctgtagcct gatcctgact agctgacgga ggattaattg
     1201 gcgactaccc cacgacttgc tcaaggtgct gcaaaaggct tcccctggag ccgtttgtgg
     1261 ttcttgctgg cagcattggt tgtataccgc atcggtgcgc atattcctgt tccaggaatc
     1321 gacccggcgc agttggcgca gttattcaaa caaaaccagg atggcatcct ggggatgttt
     1381 aacatgtttt ctggcggtgc cctgtcgcgt ttcacgattt tcgcccttgg tatcatgccg
     1441 tacatctcgg cgtcgatcat catgcagtta ttgtcggtga tatcgccgca gcttgaagct
     1501 ttgaaaaaag aaggtgaagc tggtcgtcgc aagatcaccc agtacactcg ctacggtact
     1561 ctggtgctgg caacgtttca ggcgctgggc attgctgtgg cgctggagtc gcaggccggt
     1621 cttgtgcttg atccgggtct ggcattccgt ttgacgacgg ttgtgacgct ggtgaccggc
     1681 acgatgtttc tgatgtggct gggggagcag atcacggaac gtggtttggg taatggtatt
     1741 tcgatcatta ttttcgctgg tatcgcagct gggttgccga atgcgttggg tggtttgttt
     1801 gaactggttc ggaccggttc gatgagtgca ttgttggcaa ttctgatttg tttgatcgtc
     1861 gctctggtaa cctttctggt ggtgttcatt gagcgcggtc agcgcaagat tctggtgaat
     1921 tatgcgaagc gtcaggtggg taacaaaatt tacggcggtc aaagcagtca tttgccgctg
     1981 aagctgaata tggctggtgt gattcctccg attttcgctt cgtcggtcat cttgtttccg
     2041 gcaacgatca cgagctggtt tacctctgga gacacctcga atcctttcat tcgtttcctg
     2101 aaggatttgg cggcatcgat ggcgccaggc gaaccgattc acgcactgct ctatgcgatt
     2161 gctattgttt ttttctgttt tttctatact gcgctggtat tcaacagtaa agaaactgca
     2221 gataacctga aaaagagtgg agcattcgtt ccaggtattc gtcctggcga gcagacggcg
     2281 cggtatatcg acaagatcct gatgcgcttg accttggttg gtgcgatgta cattacactg
     2341 gtttgtttgt tgcctgaatt tctgatcaca cgctggaagg tgccttttta ctttggggga
     2401 acctcgcttt tgatcatcgt ggtcgtcacc atggatttca tggtgcaggt tcagaattat
     2461 gttatgtcac agcagtatga atcgttgctc cgtaaggcaa atttcaaggg cggtatgact
     2521 tcacgttaag tgaaaacttc gtccaaagga acaagaccga atggcaaaag acgacgttat
     2581 tcagatgcaa ggtgagattc tggaaaatct gccgaatgcg acattcaaag ttaagctgga
     2641 aaacggacac gttgtactcg gccatatttc cggcaggatg cgaatgaact atatccgtat
     2701 cctgccagga gataaggtga cagtggaact gactccttat gatttaagcc gggcgcgtat
     2761 cgtgttccgt acgaaataaa cctagtattg gaagaaagag ggccacaatg aaagtgctcg
     2821 catcagtcaa gcggatctgc cgcaactgta aaatcatcaa gcgcaaaggc gtgattcgtg
     2881 tgatttgcac ggagccgcgt cataagcagc gccaaggtta atttaatgtt attgatcgag
     2941 gaataaggca tggcacgtat tgcaggggta aacatcccca accatcagca catcgtcatc
     3001 ggcctgacag ccatttacgg tgtcggccgt tcacgcgcag aaaagatctg tgttgcgaca
     3061 ggggtttcga ctagcaagaa agtcaaggat ctgaacgata gcgagctgga aaagcttcgt
     3121 gacgaaattg caggattcgt cgtcgaagg
//
LOCUS       DASRDH010000016         3308 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1027037,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000016 DASRDH010000000
VERSION     DASRDH010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 3308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3308
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1027037"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1200)
                     /gene="glyA"
                     /locus_tag="VFA14_00385"
     CDS             complement(<1..1200)
                     /gene="glyA"
                     /locus_tag="VFA14_00385"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HZS79881.1"
                     /translation="MFAKNHTIDNVDPELWSAIRKENVRQQEHIELIASENYTSPAVM
                     EAQGTQLTNKYAEGYPGKRYYGGCEFVDVVEQLAIDRLKQLFGAEAANVQPNSGSQAN
                     QGVFFAMLKPGDTIMGMSLAEGGHLTHGMALNMSGKWFNVVSYGLNDREEIDYAAMES
                     LAREKNPRLIIAGASAYALRIDFERFARIAREVGAYFMVDMAHYAGLIAAGVYPNPVP
                     HADFVTSTTHKSLRGPRGGVILMKAEHEKAINSAIFPGIQGGPLMHVIAAKAVAFKEA
                     LAPEFRAYQEQVVRNADALARTLIARGLRIVSGRTESHVMLVDLRAKKITGKEAEAIL
                     GTAHMTCNKNAIPNDPEKPFVTSGIRLGSPAMTTRGFKEAEAVKVGNLIADVLDDPHD
                     AAAIERVK"
     regulatory      complement(1354..1457)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01750"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ZMP/ZTP riboswitches appear to regulate genes
                     involved in one-carbon metabolism; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="5-aminoimidazole-4-carboxamide
                     ribonucleotide (ZMP)/
                     5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranosyl
                     5'-triphosphate (ZMT)"
                     /db_xref="RFAM:RF01750"
     gene            1601..2350
                     /locus_tag="VFA14_00390"
     CDS             1601..2350
                     /locus_tag="VFA14_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008326553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZS79882.1"
                     /translation="MIVLITGATAGFGAGMARRFVRHGHKVIAAGRRKDRLDALASEY
                     GDKVLPLALDVTDKISMQSALAGLPQGWQDIDVLINNAGLALGLEPAHQATLEDWETM
                     IDTNVKGLVTMTHHILPGMIRRGAGTIINLGSIAGTYPYPGSNIYGATKAFVEQFTLN
                     LRADLVGTGVRATNLAPGLCGGTEFSSVRFRGDADAVAKVYEGTTPLTAEDIAETVYW
                     IATLPPHVNINHIEMMPTCQGFGPLNIKRAQ"
     gene            2373..3062
                     /gene="tolQ"
                     /locus_tag="VFA14_00395"
     CDS             2373..3062
                     /gene="tolQ"
                     /locus_tag="VFA14_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007584943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HZS79883.1"
                     /translation="MNVTQDLSFFALIANASVLVQLVMLLLLLASVFSWTYIFHKMLT
                     LRRARLQTEKFERVFWSGGNLIALYQKIASNRRKSGALERIFQAGLIEFDKARKQTGK
                     GATIDASGLLDGARRAMRAAYQREMDALESHLAFLASVGAVSPYVGLFGTVWGIMNAF
                     RGLANMQQATLAAVAPGIAEALIATAIGLFAAIPAVVAYNRYSHDVDRLAIRFENFIE
                     EFSNVLQRQAH"
     gene            3062..>3308
                     /locus_tag="VFA14_00400"
     CDS             3062..>3308
                     /locus_tag="VFA14_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008326548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HZS79884.1"
                     /translation="MAGLSMHGRRTRKFKSEINVVPYIDVMLVLLIIFMAAAPVNDPS
                     VINLPTTGKSTAPPDEYIEIALKPDTKATIKINGHKHD"
BASE COUNT          726 a         1009 c          899 g          674 t
ORIGIN      
        1 cttcacacgc tcgatggcag cggcatcatg cggatcgtcc agcacgtcag caatcagatt
       61 gccaaccttg acggcttcag cttccttgaa gccgcgcgtg gtcatcgccg gactgcccag
      121 gcgaataccg gaagtcacga acggcttctc tggatcgttc gggatggcgt tcttgttgca
      181 ggtcatgtgc gcggtgccga ggatggcctc agcttccttg ccggtgattt tcttggcacg
      241 cagatcgacc agcatgacat gcgactccgt acgcccggag acgatgcgca gaccgcgcgc
      301 aatcaaggtt ctggccagcg catcggcatt tctgacgacc tgttcctgat acgccctgaa
      361 ttccggcgcc agcgcttcct tgaaagcgac tgccttagcg gcgatgacgt gcatcagcgg
      421 accgccttga atgcctggaa aaatggcgga gttgatggct ttttcatgct cggctttcat
      481 caggatcacg ccgccgcgcg gaccacgcag ggatttgtgt gtcgtcgagg tgacgaaatc
      541 ggcgtgagga accgggttgg gataaacacc ggcagcaatc aggccggcat agtgcgccat
      601 atcgaccatg aagtaagcgc cgacttccct ggcgatcctg gcaaaacgct cgaagtcgat
      661 gcgcaatgca taagcggaag cgccggcgat gatcagcctg gggtttttct cacgcgccag
      721 actctccata gctgcgtaat cgatctcttc cctatcattc agaccgtaag aaacgacatt
      781 gaaccatttg ccggacatgt tcaacgccat gccatgcgtc agatggccgc cttccgccag
      841 cgacatgccc atgatggtgt cgcccggctt cagcatggcg aagaacacgc cttgattcgc
      901 ctgcgaaccg gaattcggct gcacgttggc ggcttcggcg ccgaacagtt gcttcaggcg
      961 atcaatcgcc agttgttcga cgacgtcgac gaattcacaa ccgccatagt aacgcttgcc
     1021 cggatagcct tcagcgtatt tgttggtcag ttgggtccct tgcgcttcca tgactgccgg
     1081 cgacgtgtag ttttcggaag cgatcagctc gatgtgttcc tgctgacgga cgttttcttt
     1141 ccggatagcg gaccatagtt ccggatcgac attgtcgatg gtgtgatttt ttgcaaacat
     1201 gatttctcca agggataagg acgcgcctga aggtaaatcc gatcggtcac accttgagag
     1261 accatgggca acgccgactt gatcgacttt caaatcgaat gaggtccaaa aacaaaaaag
     1321 ggaatggcag gaagcctcga cctgcgttcc attcagggct gcccaggcgc acggctaatg
     1381 caatctcacg cttcctgatg gatatccatc ttgtaccgga acgaggcccg gatttttcgc
     1441 cagtcacgtg agacgaaata attcacgcat tgtaagtgaa agctcccgat tcggcaagat
     1501 ttgcgccagc ccgaaccaat cacgcccagc agcgctttgc gctacaattc caagttgctg
     1561 atgcagatgc atcgtcctgg catcaacagg gggaacaggg atgatcgttt tgattacggg
     1621 tgcgacagca ggcttcggcg ccggaatggc gcgcaggttc gtacgacatg gccacaaagt
     1681 cattgccgcc ggtcgccgca aggaccgtct cgacgcactg gctagtgaat acggagacaa
     1741 ggtgctgccg ctggcgctgg atgtcaccga caaaatctcc atgcaatccg cgctggccgg
     1801 gctaccgcaa ggctggcaag acatcgacgt actgatcaat aacgccggcc tagcgctggg
     1861 tctggaacca gcccatcagg caacgctgga agactgggaa accatgatcg ataccaacgt
     1921 caaaggcttg gtcaccatga cgcaccacat tctgcccggc atgatcaggc gcggcgcagg
     1981 cacgatcatc aatcttggtt cgatcgccgg cacctatccc tatccaggta gcaatatcta
     2041 tggcgcgacc aaggcattcg tcgaacagtt cacgctgaac ctgcgcgccg atctggtcgg
     2101 caccggagta cgcgccacca atctggctcc cggcctgtgc ggcggcaccg agttttccag
     2161 cgtgcgtttc agaggcgacg ccgacgcggt ggccaaagtc tacgaaggca ctaccccgct
     2221 gaccgccgaa gacatcgccg aaaccgtgta ctggatcgct acactgccgc cgcacgttaa
     2281 catcaaccat atcgaaatga tgccgacctg ccagggtttc ggaccgctga atatcaaacg
     2341 cgctcaataa aactccagaa accccgcccc ctatgaacgt cactcaagat ctctccttct
     2401 ttgccctgat cgccaatgcc tctgtactcg tgcagctggt catgctgctt ttgctgctgg
     2461 catccgtttt cagctggact tacatcttcc acaagatgct cacgctgcgc agggcgcgtc
     2521 tccagaccga aaaattcgag cgtgtgttct ggtccggcgg caacctgatc gcgctatatc
     2581 agaagatcgc gtccaaccgc cgcaagagtg gcgcgctgga acgcatcttc caggctggcc
     2641 ttatcgaatt cgacaaggcc aggaagcaga ccggcaaagg cgcgacgatc gatgccagcg
     2701 gcctactcga cggcgcccgc cgcgccatgc gcgccgctta ccagcgtgaa atggacgcac
     2761 tggaatcaca cctggccttt ctcgcatcag tcggcgcggt ctcaccctat gtcggtctat
     2821 tcggcacggt atggggcatc atgaacgcct tccgcggtct ggccaatatg cagcaagcga
     2881 cactggcagc agttgcgccc ggcattgccg aagcgctgat cgccaccgcc atcggcctgt
     2941 tcgccgcgat ccctgccgtg gtagcctaca accgctactc acatgacgtc gatcgcctgg
     3001 cgatccgctt cgagaacttc atcgaagaat tctccaacgt cctgcaacgt caggcgcact
     3061 gatggcaggc ctgtccatgc acgggagacg cacccgcaaa ttcaagtcgg aaatcaacgt
     3121 cgtgccctac atcgacgtga tgctggtgct gctcatcatc tttatggcgg ctgcgccggt
     3181 caatgaccca agtgtcatca acctgccgac taccggcaag tcaacggcgc cgcccgacga
     3241 atacattgaa atcgccctca agcccgacac caaggctacc atcaagatca atggccacaa
     3301 gcatgaca
//
LOCUS       DASRDH010000017         2186 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1132911,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000017 DASRDH010000000
VERSION     DASRDH010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1132911"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(128..202)
                     /locus_tag="VFA14_00405"
     tRNA            complement(128..202)
                     /locus_tag="VFA14_00405"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(168..170),aa:Val,seq:cac)
     gene            419..1105
                     /locus_tag="VFA14_00410"
     CDS             419..1105
                     /locus_tag="VFA14_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012205573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HZS79885.1"
                     /translation="MRRGLVDALPVMAGFIPFALVLGAQATQKGFSALQVLLLTGLNF
                     GGGSEFAAVALWTSPPHVLLIVAITFLVNSRHLLMGAVLAPFLRHLPKRKVLPALFFM
                     CDESWAMGLADARQRAQAGKRHAFSLRYYFGVSLGLYLTWIAFTTIGAIAGPLIGDIE
                     TWGFHMAFPAVFLVLLRGMWKGLRAACPWLVSLTVAAATYLVAAGAWYVATGALSGLA
                     AAYALAGIRT"
     gene            1102..1401
                     /locus_tag="VFA14_00415"
     CDS             1102..1401
                     /locus_tag="VFA14_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015601653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD family protein"
                     /protein_id="HZS79886.1"
                     /translation="MMHASTLLTIVLMATVTYLTRALGYLVLRNRSLSERAVTVMEAA
                     PGCVLISVIAPYFVSERPTDLVALAATLLAATRLPMLPTVIIGVAVSGLLQHITG"
     gene            complement(1578..>2186)
                     /locus_tag="VFA14_00420"
     CDS             complement(1578..>2186)
                     /locus_tag="VFA14_00420"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013213088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HZS79887.1"
                     /translation="LTGSTNIAYLKENGVKIWDEWADEQGDLGPIYGYQWRSWPAPNG
                     EHIDQIAQVVSQIKSTPDSRRLIVSAWNVADIPQMKLPPCHAFFQFYVADGKLSCQLY
                     QRSADIFLGVPFNIASYALLTHMVAQQTGLEVGDFIWTGGDCHLYANHMEQVKEQLTR
                     APGPLPKLTIERKPDSIFDYGFDDFDISGYEAQTHIRAPVAV"
BASE COUNT          424 a          636 c          648 g          478 t
ORIGIN      
        1 tgatcgccgg cgccgcgatg gcgatctcga gcatcagtgt cgtcagcaat acgttattgc
       61 tcaaacgctg gaaaccgcaa gaagaaaaac ggaatattta ggaatgcaca ctggccggaa
      121 tgcatactgg tggaaagtgc aagtttcgaa cttgcgaccc ctgcagtgtg aatgcagtgc
      181 tctacccctg agctaacctt cctagtgtga aacggcctgg attatggcat gcattgccgc
      241 tccaagcaag ctcccggaag aacttcagga ttccgcgcga agccgtagcc gctacaacgt
      301 ggatgccaga tcaaatccaa gttaggataa tagtccagca atcacaccag acttcttttt
      361 tatggaacaa cgcgcctccc ccattgccat ctcctcgcct gaacacaagt gccgtgaaat
      421 gcggcgcggc ctcgtggacg cgctgccggt catggctgga ttcattccct tcgccctggt
      481 gcttggcgcc caggcgacac aaaagggatt cagcgcgctg caagtgctat tgctgaccgg
      541 actcaatttt ggcggtggtt cggaattcgc cgccgtggcc ctctggactt cgccgccgca
      601 cgtgctgctg atcgttgcga tcaccttcct ggtcaacagc cgccacttgc tcatgggggc
      661 tgtactggcg ccttttcttc ggcatctgcc caagcgcaaa gtgttgccgg ccttgttctt
      721 catgtgcgat gaaagctggg ccatggggct ggccgatgcg cgccagcgcg cgcaagccgg
      781 taagcgccat gccttcagcc tgcgttatta tttcggcgtc tcgctgggac tgtatctgac
      841 ctggatcgcg ttcaccacca tcggcgccat cgcagggccg ttgatcggag acatcgagac
      901 ctggggcttt catatggcgt tcccggcagt attcctggtt ttgttgcgag gcatgtggaa
      961 ggggctgcgc gccgcctgcc catggctggt cagcctgacg gtcgcagccg ctacctatct
     1021 ggtagcggct ggcgcctggt atgtcgcgac gggcgcgctg tcaggactgg ccgcagctta
     1081 tgccctggca gggatccgga catgatgcac gcgagtacct tgctgaccat cgtcttgatg
     1141 gcgaccgtga cctacctgac ccgcgctttg ggctacctcg tcctgcgcaa ccgcagcttg
     1201 agcgagcggg ccgtgacagt gatggaagcg gcacccggat gcgtgctgat ctcggtcatc
     1261 gccccgtatt tcgtatcgga acgcccaacc gacttggtcg ccttggccgc aaccctgctt
     1321 gccgcaaccc gtctgccgat gctgccgacg gtcatcatcg gcgttgcggt cagcggcctg
     1381 ttacagcaca tcaccggata aatcttcctg atatcagaac ctgttgacga tttcatcggg
     1441 agtcgtccgg ttattttttc aggatgggat gcaaggcgta aactgcagac aggctggcgg
     1501 ctcgacgagg attggcaacg cagcagaccg cctgaaagag cgaccagatg gcctcgcagg
     1561 aggtcgtcaa caagctctca gaccgcgacc ggcgccctga tatgcgtctg tgcttcgtag
     1621 ccgctgatgt cgaaatcgtc gaatccatag tcaaaaatgc tgtccggctt gcgctcgatg
     1681 gtcaacttcg gcagcgggcc gggcgcgcgc gtgagctgct ctttgacttg ctccatgtgg
     1741 ttggcgtaca gatgacaatc gccgccagtc cagatgaagt cgccgacctc gaggccggtc
     1801 tgctgcgcga ccatatgcgt gagcaaggcg taggaagcga tattgaacgg cacgccgaga
     1861 aaaatgtcgg cgctgcgttg atacaactgg caggagagct tgccgtcggc gacgtagaac
     1921 tggaagaaag catggcaggg tggcaacttc atttgcggga tgtcggccac gttccatgcc
     1981 gatacgatca ggcgacgcga atcgggcgtg cttttgatct ggctgacgac ttgcgcgatc
     2041 tgatcgatat gttcaccgtt gggcgccggc catgaacgcc actggtaacc gtaaatcggc
     2101 cccagatcgc cttgctcatc ggcccattca tcccagattt tgacgccgtt ttctttcaga
     2161 taggcaatat tggtcgagcc ggtgag
//
LOCUS       DASRDH010000018         8975 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_670786,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000018 DASRDH010000000
VERSION     DASRDH010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 8975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8975
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_670786"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..432)
                     /locus_tag="VFA14_00425"
     CDS             complement(76..432)
                     /locus_tag="VFA14_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NirD/YgiW/YdeI family stress tolerance protein"
                     /protein_id="HZS79888.1"
                     /translation="MRTSYMRVAVFSAALALTAPVFAQYIGPQKRVPLVTVTAAASLS
                     NNTYVMLEGHIISRIRKEHYTFKDATGSIEIEIDDKHFPSSRPIDEKTKVRIHGKIDK
                     DFIKDATIDVEQVEVL"
     gene            complement(494..946)
                     /gene="cadR"
                     /locus_tag="VFA14_00430"
     CDS             complement(494..946)
                     /gene="cadR"
                     /locus_tag="VFA14_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006465366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cd(II)/Pb(II)-responsive transcriptional
                     regulator"
                     /protein_id="HZS79889.1"
                     /translation="MHTLKIGELAARTDCPVETIRYYEREALLPEPVRSLGNYRLYGD
                     THVERLQFIRHCRALDMTLDEVRSLLRFRDAPAENCGEVNALLDKHIGHVVQRIRALK
                     KLQGQLQTLRAQCDTAQAVEDCGILQGLVNMRDDSRPVNLGSHGNGCH"
     gene            1054..3264
                     /locus_tag="VFA14_00435"
     CDS             1054..3264
                     /locus_tag="VFA14_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HZS79890.1"
                     /translation="MPHCSHHNHTHEHIRQHDADDIEPAALKNVQKAVFFIQNMDCPT
                     EEKLIRTRLAGMEGIAAMQFNLIQRELTVQHRLTSIAPIVAALSALDMLPRIKSDTLS
                     AGSDAADRLARAMPKRKWRRVGIAAIAALAAEAVAWNSGNDGSPTVIALALFAIATGG
                     GATLRKGWIALRHLSLNMNFLMSLAVIGAILIGQWPEAAVVIVLFTLAEMIESLSLDH
                     ARNTIAGLMAMSPDQATVLRDGVWTSIAAGQVAVDAEVRVKPGERIPLDGIIMQGQPT
                     IDQAPITGESIPVVKTGGDQVFAGTINGNTAFDYRVTAVQGESTLARIIHSVQQAQGR
                     RAPTQRLVDRFARYYIPAIVALAALVALAPPLLLGVDFYPWFYQALVLLVIACPCALV
                     ISTPVTIVSGLAAAARHGILIKGGIYLEQGRRLEAIAFDKTGTITHGKPVVTDTQVLS
                     QDAAATLLQQAASLALRSDHPVSIAIAAHWQAQQTGAATDIADFEALPGRGVKGRIGG
                     DACYLGNHRLVHDMGMCNPALEERLQILEKQGKTVTILCRDGRAQLLLAVADTVRDSS
                     RDAIAQLRRSGISVTILTGDNPRVARAIANVIGIDDARGDQLPADKLDAIDGMIEQHG
                     YVGMVGDGINDAPALARAHIGFAMGAAGTDTALETADVAIMNDDLRRIPDFIRLSQKT
                     HGVLMQNITIALGIKALFLGLALTGASSLWMAVFADMGASLIVMFNGLRLMRSP"
     gene            3429..4232
                     /locus_tag="VFA14_00440"
     CDS             3429..4232
                     /locus_tag="VFA14_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006404581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZS79891.1"
                     /translation="MSKVVLVTGAAKRAGKAFVEYFARKGFDVVVHYGRSATDAEAVV
                     DVVRQTGQRAIAVHADLSSAGQITKLVDETYEAFGRLDLLVNNASVFGQDHFPDFSTD
                     NLDVSIAVNYRAPILLTQAFYRKARAQGAAGVVINVVDQKVKGNFHRDHFSYTVGKVG
                     IGNMTAMLAISAAPVLRVNAVFPGLMLQSDDQTQEDFEYASRNATPLGRIATPEDVAQ
                     AIALLTSPAYNGFDFVIDGGQNLSRVGQDVLYQYRARHDASASASDAAQ"
     gene            4186..4710
                     /locus_tag="VFA14_00445"
     CDS             4186..4710
                     /locus_tag="VFA14_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012402677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HZS79892.1"
                     /translation="MPDTTPQHQPAMLRNNKVIAGLLCWFAFAAAQAQDEMLTVTGSI
                     MGHTDGHTNSYVFSENDLFALGQHIIKTSTSWTPESSFTGPRLSVVLSKAGAIGTHVE
                     VHTLDDYTYTISVDEADRYGAILAHTMNGKKLQVSDFGPLFLIYPRDQYPHELRTPVA
                     EAKFIWQIRKLIVK"
     gene            4753..5247
                     /locus_tag="VFA14_00450"
                     /pseudo
     CDS             4753..5247
                     /locus_tag="VFA14_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007877906.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
     gene            5368..5688
                     /locus_tag="VFA14_00455"
     CDS             5368..5688
                     /locus_tag="VFA14_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018310634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HZS79893.1"
                     /translation="MQPNYKRPFSQYVKKAHKPLQLAIEDAVEDVCANPDIGVAKTGD
                     LAGIWVYKFTFNRQEYLMAYRPPTDEQRRQEIKLELLMIDFYQLGAHENFYDDLKRYL
                     KAER"
     gene            5693..6031
                     /locus_tag="VFA14_00460"
     CDS             5693..6031
                     /locus_tag="VFA14_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013958057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HZS79894.1"
                     /translation="MSTMTAEELFHDFKTMPANERVRFFSLLAINAFGDENANHEQVF
                     GHLANDEFTAHDAAEYLEVSMSTFRRYVHDGKIQPSSELGRNQLFAARNLKIFKRALK
                     DVKRTLEATA"
     gene            complement(6033..6503)
                     /locus_tag="VFA14_00465"
     CDS             complement(6033..6503)
                     /locus_tag="VFA14_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS79895.1"
                     /translation="MSRLLVLVAGLNNLNRRGNHDSFAPSKGAPASLADFADRPLGGK
                     LQQIDQSWYRIDAGAIDNRLRNSIAHYKVEYDEITQELVYYPKQEGLEQAIAMRMHFL
                     DFSRKILEVFRELHRLNHLTKCLFVYYYLKMVGVSGTDIDANDEADTLKLPPPA"
     gene            complement(6609..7184)
                     /locus_tag="VFA14_00470"
     CDS             complement(6609..7184)
                     /locus_tag="VFA14_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HZS79896.1"
                     /translation="MSQVAIVYHSGYGHTKKQAEAVCAGAASAAGARVELIAISAEGS
                     ISDADWAMLDAADAIIFGSPTYMGSVSWQFKKFADASSKPWAAQKWKDKIGAGFTNSA
                     TMNGDKLLTLHALFTLGMQHSMIWVGTGLMPSNAKAAQRNDINFVGSFCGLMAQSPSD
                     ASPEEGPLPGDLETARLFGRRVADIAARFKK"
     gene            7318..8217
                     /gene="gluQRS"
                     /locus_tag="VFA14_00475"
     CDS             7318..8217
                     /gene="gluQRS"
                     /locus_tag="VFA14_00475"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HZS79897.1"
                     /translation="MPLEHYVGRFAPSPSGPLHAGSLVAAMASYLDARAHRGQWLLRV
                     EDIDETRTVNGAAQDIETALQQLGMHRDGKLLVQSQRKQRYQQVCERLGTLVYPCGCT
                     RREIADFRLGIATDGAAIYPGTCRNGPAPGKTARALRVRVPERGQDREYIRFRDRWSG
                     WQSQHLAQATGDFLLKRADGFWAYQLAVVVDDAEQGVTHIVRGADLLDSTARQIYLQH
                     LLSYPTPAYLHIPLVRHSDGEKLSKQNGAQALDLTRPLDALHTAATFLGLELATANTV
                     STFWERALPAWQRRLDQLEDSAP"
     gene            8214..8732
                     /locus_tag="VFA14_00480"
     CDS             8214..8732
                     /locus_tag="VFA14_00480"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017407759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HZS79898.1"
                     /translation="MNVQIRHEIPDDIAAIEAVTIAAFATAPHTSRTEQFIVRALRAA
                     NALTLSIVAEEHGQIIGHATLSPVTITDAHGKQTAGWHGLGPISVLPARQGQGIGSRL
                     MKQALTELRAQHAAGCVLLGEPAYYTRFGFQSHAGLQLPGMPSACFMALALRGTVPEG
                     IAQYSDAFNAAA"
BASE COUNT         1984 a         2604 c         2532 g         1855 t
ORIGIN      
        1 gcgcgtggag aagtcgatgg ggaaaataag cgctatcggc actggtgtca atagcatgcg
       61 gctgtctgca aaaatttaca gcacttccac ctgttcgaca tcgattgtgg cgtctttgat
      121 gaaatccttg tcgatcttgc catggatacg gactttggtt ttttcatcga tgggcctgga
      181 agacggaaaa tgcttgtcgt cgatttcgat ttctatcgat ccggtggcat ccttgaaagt
      241 gtaatgctcc ttgcgaatcc tcgagatgat atgaccttcc agcatcacat acgtattgtt
      301 cgacaaactg gctgccgccg tcaccgtgac cagcggcacc cgcttttgcg gtccgatata
      361 ttgcgcaaag accggcgcgg tcaaagccag cgcagcggaa aaaacagcga cacgcatata
      421 agatgttctc atcagacagt actcctcggt taaaccggtg ttggactacc tctccagaaa
      481 atggttcacg ccatcagtgg catccgttac cgtgacttcc cagattgacc ggcctgctgt
      541 cgtcacgcat attgaccagg ccctgcagga tgccgcagtc ctcgacggcc tgggcagtgt
      601 cacattgcgc acgcagcgtc tgcaactgtc cctggagttt tttcagcgcg cggatgcgct
      661 ggacgacatg accaatatgc ttgtcgagca aggcattgac ctcgccgcag ttttccgccg
      721 gcgcatcacg gaaccgcaac agactgcgaa cctcatccag cgtcatgtcg agcgcgcgac
      781 aatggcggat gaattgtaga cgctcgacat gcgtgtcgcc atataaacga taattgccca
      841 gcgagcgcac gggttccggc agcaaggctt cacgctcgta ataacggatg gtttccaccg
      901 gacaatcggt gcgcgccgcc agttcgccta tcttcagggt atgcatattt tcctccagga
      961 aaccggcttg tgaaataaac cccttgaccc tatagccagt tcagggtttt caatatacag
     1021 caaacccttc tattctgcct gtcaggaata cgcatgccgc attgcagcca tcacaaccat
     1081 acgcacgaac acattcgtca gcatgatgcc gacgacatcg aaccggccgc tctgaagaat
     1141 gtccagaaag cggtattctt catccagaac atggattgcc cgactgaaga aaaactgatc
     1201 cgcacacgcc tcgccggcat ggagggcatc gccgcgatgc agttcaacct gatccagcgc
     1261 gaactgacgg tacagcatcg cctgacttcg attgccccta tcgtcgccgc actcagcgca
     1321 ctcgacatgc tgcccaggat caagagcgac acgctcagcg ctggcagcga cgccgctgac
     1381 cgcctggccc gcgccatgcc gaagcgcaaa tggcgcagag tcggcatcgc cgccatcgcc
     1441 gcgctggccg ccgaagccgt ggcctggaac agcggcaatg acggatcgcc gacggtcatc
     1501 gcgctggcct tgttcgcgat cgccaccggc ggcggcgcta cgctcaggaa aggatggatc
     1561 gcgctgcgcc atctgtcgct gaacatgaac ttcctgatgt cgctggcggt cattggcgcc
     1621 atcctcatcg gccagtggcc ggaagcagcg gtggtcatcg tcctcttcac cctcgcggaa
     1681 atgatcgaat cgctatcgct ggaccacgcg cgcaatacga ttgccggact gatggcgatg
     1741 tcacccgatc aggccacagt gctgcgggac ggcgtctgga ccagcatcgc cgccggacag
     1801 gtggcagttg acgccgaggt gcgcgtcaaa ccgggtgaac gcattccact cgacggtatc
     1861 atcatgcaag ggcagccgac gatcgaccag gcgccgatca ccggcgaaag tataccggtc
     1921 gtcaaaacag gcggcgacca agtatttgcc ggcaccatca acggcaatac ggctttcgac
     1981 tatcgcgtca ccgccgtgca aggtgaatcg acgctcgcgc gcatcatcca cagcgtgcaa
     2041 caggcgcaag gccggcgcgc gccgacgcaa cgcctggtcg atcggttcgc gcgctactac
     2101 attccagcca tcgtcgctct ggcggcgctg gtcgccctgg cgccgcccct gctgcttggc
     2161 gtcgactttt atccctggtt ttaccaggct ctggtgctgc tggtcatcgc ctgcccctgc
     2221 gcgctggtga tttccactcc ggtgaccatc gtcagcggac tggcagcggc ggcgcgtcac
     2281 ggcatcctga tcaagggcgg catctacctg gaacagggtc gccggctcga ggccatcgcg
     2341 ttcgacaaaa ccggcaccat cacgcatggc aagcctgtcg tgaccgacac gcaagtgtta
     2401 tcgcaagacg cagcagccac gctgctgcaa caagccgcca gtctggcgct gcgttcggat
     2461 catccggtgt cgatagcgat cgccgcgcat tggcaggcgc agcaaacagg cgcggcaaca
     2521 gacatcgccg acttcgaggc gctccccgga cgcggcgtca aaggccgcat cggcggtgat
     2581 gcatgttatc tcggcaacca tcgcctcgtg catgacatgg gcatgtgcaa cccggctctg
     2641 gaagagcgat tgcagatcct ggaaaaacaa ggcaaaaccg tcaccatcct gtgccgtgac
     2701 gggcgcgcgc agttgctgct ggccgttgcc gacacggtgc gcgacagcag tcgcgacgcc
     2761 atcgcacaac tgcgtcgctc aggcatcagc gtcaccatac tgaccggcga caatccgcgt
     2821 gtcgcgcgcg ccatcgccaa tgtcatcggc atcgacgatg cgcgcggcga tcagttaccg
     2881 gcagacaaac tcgacgccat cgacggcatg atagagcagc acggctatgt cggcatggtc
     2941 ggcgacggca tcaacgatgc gccggcgctg gcgcgcgcgc acatcggttt cgccatgggc
     3001 gcggccggca ccgacacggc actggagacc gccgacgtgg ccatcatgaa tgacgacctg
     3061 cgcaggattc ccgacttcat ccgcctgagc caaaagaccc acggtgtgct gatgcaaaac
     3121 atcacgattg cgctcggcat caaggcgctc ttcctgggac tcgcgctgac cggcgcaagc
     3181 tcgctatgga tggcggtatt cgccgacatg ggcgcgagcc tgatcgtgat gttcaacggc
     3241 ttgcggctga tgcgttcgcc gtaacctgca taaccagtcg tcttttttca cacctgcaaa
     3301 acagcgtccg tataatggtg aaattttcag cctggtggac aacataccat gaagatcagt
     3361 ctcgatacgc tctacaagca cacggcaaga ggaagcgatg gtggcacttt tgatgaaggt
     3421 gaatcaagat gagcaaagtt gtcctggtga cgggtgctgc gaaacgtgca ggcaaagcat
     3481 ttgtcgagta ttttgcaaga aaaggctttg atgtcgttgt tcactatggg cgttccgcga
     3541 ccgatgccga agcggtcgtt gatgtagtca gacagactgg tcaacgagcc attgcagtac
     3601 atgcagacct gagctctgct gggcaaatca cgaaattggt tgacgaaacg tatgaagcct
     3661 tcggcaggct tgatctgttg gttaacaacg catcggtttt cggtcaggat cacttcccgg
     3721 atttttcgac ggacaacctg gatgtctcca ttgctgtcaa ttatcgtgct ccgattttgc
     3781 tgacccaggc gttttatcga aaagcccggg cgcagggtgc agcaggggtc gtcatcaacg
     3841 tcgttgacca gaaggtcaaa gggaatttcc atcgggatca cttttcttac actgttggta
     3901 aggtcggcat tggcaatatg acggccatgc ttgccatatc agccgcgccg gtattgcgcg
     3961 tgaacgcagt atttccagga ctgatgctgc aaagcgatga tcaaacgcag gaggattttg
     4021 agtacgccag caggaatgcg acaccgcttg ggcggattgc gacgcctgag gacgttgcac
     4081 aagccattgc tctgctgacc agtccggcgt acaacggctt tgattttgtg atcgatggcg
     4141 gtcaaaatct gtcgcgcgtc ggacaggacg tgctgtatca ataccgtgcc cgacacgacg
     4201 cctcagcatc agccagcgat gctgcgcaat aataaagtga tagccggcct gctttgctgg
     4261 tttgcctttg ctgccgccca ggcgcaggat gaaatgctta cggtgactgg atcgatcatg
     4321 ggccataccg atggccatac gaatagttac gtcttttcgg agaacgacct tttcgccctg
     4381 gggcagcata ttatcaagac ttccaccagc tggacgccag agtcttcgtt tacggggcca
     4441 cgattatcag tcgtattgtc gaaggccggc gccatcggca cgcacgtcga agtgcatact
     4501 ttggatgact atacgtacac catatccgtc gatgaggcgg atcggtatgg cgcgatcctg
     4561 gctcacacca tgaacggtaa gaagctacag gttagcgatt tcggaccgct gttcctgatt
     4621 tacccgcgcg atcaatatcc acacgagttg cgcacgcccg ttgctgaagc aaagttcatc
     4681 tggcaaatca ggaaattgat cgtgaaatga gccaggaatg catttttagc ggtggttagc
     4741 catgaactgc gcgtggcggt ggccgggcgc ggcgtcaagg cggtggaaga cgccaccaag
     4801 ggcatgcatg caattcacag gcagattcag gagatctgca aatgcaccaa acgtcttgga
     4861 gaatcgtcgc aagagatcag caacatcatc gaactggttt ccgatatgac cgaacaggcc
     4921 agtgtgctgg ccgtcaatgc ttccatccag gccgcgtccg caggggaagc cgggcgcggt
     4981 ttttcggtcg tggcggaaga ggcccagcgg ctggccgaac gttccagaac cgccgtccag
     5041 cagattggtg cgctggcgcg catcatgcag tcggataccg tcgatgtcgt cgctaccatg
     5101 ggaaaatcca ccgctggcgt ggtagaagtc accagactat cggatgccgc agctgcttcc
     5161 ctgttcgaca tacgcaacat gtcgaatagg ctcgaagaac tggttcggga catttcgctg
     5221 gcaaacgacc ggcaagtcac ttcggccttc agatgagacc ggatttcagc gtcagttttg
     5281 atgcaacacg ctagcacgcc ctggcttcta tcttcacaaa caacattttg agttgattta
     5341 gaatcaaatt tgaactaata taaatccatg caacccaatt acaaacgacc atttagccag
     5401 tatgtcaaga aagcacacaa accgttgcag ctcgcgattg aagatgctgt cgaagacgta
     5461 tgtgccaatc cggacatagg agtggcgaag acaggtgact tggcgggaat atgggtttat
     5521 aaatttacat tcaatcgcca ggaatatctg atggcctatc gaccaccaac agatgaacaa
     5581 cggcgccaag aaataaagct tgaattactg atgatcgatt tttaccaatt gggcgcacat
     5641 gagaattttt acgatgacct caaacggtat ttgaaggcgg agagataacg acatgagtac
     5701 gatgacagct gaagagcttt tccatgactt taaaacgatg cctgcgaatg agcgcgtccg
     5761 ttttttttcg ctgctggcca tcaatgcctt tggcgatgaa aacgccaacc atgaacaagt
     5821 gtttgggcac ctggccaacg acgaatttac cgcgcacgat gctgccgaat acctggaagt
     5881 ttccatgtcc acattccggc ggtacgtaca tgatggcaag atccagccaa gtagcgaact
     5941 aggccgcaat caattgtttg ccgcccgcaa tctgaaaata tttaaacgtg ctctcaaaga
     6001 cgtgaaacgc acattggagg ctacagcctg aactaagctg gagggggcaa tttcagcgtg
     6061 tccgcttcgt cgtttgcatc aatgtccgtg ccacttacgc caaccatctt caggtagtag
     6121 tagacgaaga ggcatttggt caggtgattc agtcggtgca gctctctgaa gacctcaaga
     6181 atcttgcggc tgaaatccag aaagtgcatg cgcattgcga tggcttgctc cagcccctcc
     6241 tgcttcggat agtagacaag ctcttgggtt atctcgtcat actctacttt atagtgggcg
     6301 atggagttgc gaaggcgatt gtctatggca cccgcatcaa tgcgatacca cgattggtct
     6361 atctgctgga gctttccgcc cagtggccgg tcagcaaagt ctgcaagact tgctggagct
     6421 cccttggacg gagcaaagga gtcgtggttg ccccgcctgt ttaggttgtt caaacccgcg
     6481 accaggacaa gaaggcgcga catgatctca gccatgtact gatgttgaag ttcatgtagt
     6541 tctgggaagc tattcaaaat cttttagaca ccggcccgat ttgaacgaca ggcgctggtg
     6601 tcgtcgtttt attttttgaa tcgcgccgca atgtcagcaa cacgcctgcc gaacagtctg
     6661 gcagtttcca gatcgcccgg cagcgggcct tcttccggcg aagcatcgga tgggctttgc
     6721 gccatcaggc cgcagaaaga gccgacgaag ttgatgtcat tgcgttgcgc cgctttggcg
     6781 ttggacggca tcaaaccagt gccgacccag atcatgctgt gctgcatgcc cagcgtgaac
     6841 agcgcgtgca gcgtcaacag cttgtcaccg ttcatggtgg cggaattggt gaagcccgcg
     6901 ccgatcttgt ctttccattt ttgcgcggcc catggtttgg agctggcgtc ggcaaatttc
     6961 ttgaattgcc aggacacgct gcccatatag gtcggcgaac cgaaaatgat ggcatcggcg
     7021 gcatccagca tcgcccagtc ggcatcgctg atactgcctt cggcgctgat ggcgatcagc
     7081 tcgacgcgcg caccagcggc ggatgcggcg ccggcgcaga cagcctctgc ctgttttttg
     7141 gtgtggccat agccagagtg ataaacgata gcgacttgac tcatcacggt ttcctcgtag
     7201 ggataaaaga aatacactat atccgctcgt tttccggaaa gtaagcctct gaccggaaca
     7261 aatctttcga gaaattcaag caatcgtcgc ccgacatgag taccgacgtt attcaccatg
     7321 ccccttgaac actatgtcgg ccgcttcgcg ccctcccctt ccggcccgct gcatgccggt
     7381 tcgctggtcg ccgccatggc aagctatctg gatgcacgcg cgcatcgcgg ccaatggctg
     7441 ctgcgcgtcg aagacatcga cgaaacgcgt accgtcaacg gcgcggcaca ggatatcgaa
     7501 accgccctgc aacaattggg catgcaccgg gatggcaagc tactggtgca aagccaacgc
     7561 aagcagcgct atcagcaggt ctgcgaacgt ctggggacgc tggtctatcc ttgcggctgc
     7621 acacgcaggg aaatcgccga cttccggctg ggcatcgcca ccgatggcgc cgccatttat
     7681 cccggcacct gccgcaatgg cccggcgccg ggcaagacgg cgcgcgcgct gcgcgtgcgt
     7741 gtgccggagc gtggacaaga ccgggagtac atccgcttca gggatcgctg gtcaggctgg
     7801 caaagccagc atctggcaca agcaacaggt gatttcctgc tcaaacgtgc agacggtttc
     7861 tgggcctatc aactggcggt ggtggtcgat gacgccgagc agggcgtgac gcatatcgtg
     7921 cgcggcgccg acctgctcga ttcgacggcg cggcagattt atctgcaaca tttgctgagt
     7981 tatccgacac ccgcctatct gcacattcca ctggtccggc acagcgatgg cgaaaaactc
     8041 tccaagcaaa acggcgcgca ggcgctggac ctaacgcgtc cgctggatgc cctgcacacg
     8101 gcggcgacat ttcttggact ggaactggca acggcgaaca ccgtctccac attctgggaa
     8161 cgggcgctgc cggcctggca gcgccgtctg gatcaactgg aggactcagc tccatgaacg
     8221 tccagatccg gcacgaaatc ccggacgaca ttgctgccat cgaagcggtc acgatcgccg
     8281 cctttgccac tgcgccgcac accagccgca ccgagcaatt catcgtgcgc gccttgcgcg
     8341 ccgcaaacgc attgacactt tccatcgtag ccgaagaaca cggccagatc atcggccatg
     8401 cgacgctgtc acccgtcacg atcaccgatg cccacggcaa gcagacggcc ggctggcatg
     8461 gcctggggcc gatttctgtc ctgccggcaa ggcaaggaca aggcatcggc tcacgcctga
     8521 tgaaacaggc gctgaccgaa ctccgggcgc aacacgcagc cggctgcgtg ctgttgggcg
     8581 aacctgcgta ttacacgcgc tttggcttcc agtcccatgc gggcctgcaa ctgccgggca
     8641 tgccgtccgc ctgtttcatg gcgctggcct tgcgcggaac agtgcccgaa ggcatcgcgc
     8701 agtacagcga tgccttcaac gccgccgcct gagtggcaag cgaaggcggc ggcgctgagc
     8761 ctgcacaccg gcaaacgcgc cggcagtagt catgcgcaaa gaacctgttc ccgatctcac
     8821 tgggtacagg ttctaacgca atcaagagat tctttcatgc cctcgatcgt cttgatcagc
     8881 cgatcttgcg actatagcct agcggcgatg agtttaccca gacgctcgat gccggcgcgg
     8941 attttttccg gcggtacggt gacgaagccc aaacg
//
LOCUS       DASRDH010000019         3237 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1359828,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000019 DASRDH010000000
VERSION     DASRDH010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 3237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3237
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1359828"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="VFA14_00485"
     CDS             <1..355
                     /locus_tag="VFA14_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS79899.1"
                     /translation="SAMLIGAFVLTLTLADPIVSKISPAIGIYLGNISFSLYLLHNPI
                     GWASWAVLKRLGPTEVPFVSAVLAIILAAIASIIVAHLTHRYIELRLTQWLKTKLLPV
                     HDTQFDAASLERGTR"
     gene            466..978
                     /gene="def"
                     /locus_tag="VFA14_00490"
     CDS             466..978
                     /gene="def"
                     /locus_tag="VFA14_00490"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007881201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HZS79900.1"
                     /translation="MALLNILRYPDPRLHTVAKPVTTFDDRLRKLVADMAETMYAAPG
                     IGLAATQIDVHEQVIVIDVSDTSNVLRVFINPEIVWASEETQVYDEGCLSVPGIYDGV
                     ERPARVKVRALDADGTAFEVEADGLLAVCLQHEIDHLKGKVFVEYLSPLKRKRIKTKM
                     LKEERELKRG"
     gene            complement(1355..1531)
                     /locus_tag="VFA14_00495"
                     /pseudo
     CDS             complement(1355..1531)
                     /locus_tag="VFA14_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611586.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            1596..2561
                     /locus_tag="VFA14_00500"
     CDS             1596..2561
                     /locus_tag="VFA14_00500"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007881247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HZS79901.1"
                     /translation="MPASVLPAILQNLRLPVIAAPMFIASNPTLVAAQCKAGIVGSFP
                     ALNARPAEQLDVWLTQLQTELAACKATNPDAMIGPIAVNQILHRSNERLARDVDVCVK
                     HRIPIIISSLRAPSQAMLDTIHSYGGIILHDVISIRHAQKALEAGVDGLILVAAGAGG
                     HAGALSPFALVGEVRRFFSGPLILSGAIATGDAILSAQAMGADLAYIGSRWLATREAN
                     VSAPYRQAIIESTATDVVYTNLFTGVHGNYLKKSIINAGLDPDNLPAADKSRMNFGSG
                     GEARTWRDIWGAGQAVGLMDDVPRVAEMVTRLECEYAAARQRLSL"
     gene            2668..2871
                     /locus_tag="VFA14_00505"
     CDS             2668..2871
                     /locus_tag="VFA14_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS79902.1"
                     /translation="MTSFLRTRKDRHPKPENKDRLLRTHTPSDGNHTGRTENTLRGNS
                     NPDEVTQENSETPRGTDHDPSSH"
     gene            complement(2920..>3237)
                     /locus_tag="VFA14_00510"
     CDS             complement(2920..>3237)
                     /locus_tag="VFA14_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007881260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZS79903.1"
                     /translation="GWGALLASVSVFVAVAAVNIPMVILGFAMAGAGVAVAFPFVVSA
                     AGRHGTVALAGVATLGYSGSLAGPPVIGFLAHGFGLPAALVFIGVLCCAAALAASRAR
                     WLE"
BASE COUNT          740 a          953 c          884 g          660 t
ORIGIN      
        1 cagcgcaatg ctgatcggcg ctttcgttct tacgctgaca ctagccgacc cgatagtcag
       61 taaaatatcc cccgcgatag gcatttatct gggaaatatc tctttctctc tttacctgtt
      121 acataacccg ataggctggg ccagctgggc cgttctgaaa cggctcggtc ctacagaagt
      181 accatttgtc agcgcagtgc tcgccatcat tctggctgct atcgcatcca ttattgtcgc
      241 ccacctgact catcgataca tcgagcttcg tttgacgcaa tggctgaaaa caaaactatt
      301 gccggtacat gacacgcaat tcgatgctgc ttctctggag agggggacca gataatattg
      361 cggcaatgac aacgaaaaca gattccgccg acaaaccctt gaactggcga gaaccgccca
      421 cagctacggc tatcaggcgc ggatagcgca gcaagcaaaa aacgtatggc cttattgaat
      481 atcctccgtt acccggaccc ccggctgcac acagttgcca agcccgtcac cacattcgac
      541 gatcgtctca ggaagctggt cgccgacatg gccgaaacca tgtatgccgc gccaggtatc
      601 ggtctggccg cgacccagat cgatgtgcat gaacaggtca tcgtcatcga cgtgtccgac
      661 acgagtaatg tattgcgcgt gttcatcaat ccagaaatcg tctgggccag tgaagaaacg
      721 caggtctatg atgaaggctg tctgtcggtg cccggtatct atgacggtgt agagcgtccg
      781 gcccgcgtca aggtgcgcgc gctggacgca gacggcacag catttgaagt ggaagccgac
      841 ggactgctgg cggtatgcct ccagcacgaa atcgatcacc tcaaaggcaa agtcttcgtg
      901 gaatacctgt cgccgctcaa gcgcaagcgc atcaagacca aaatgctgaa ggaagaacgc
      961 gaactcaaac gcggatgacg cgcgtgatga tgaattgccc cggaacttct aggcactctg
     1021 cagcttctaa gaatactgtt tttaaaatcc tataagatga tctgggcgta tgcgtataac
     1081 gaaggactca acgacgcaaa aaaactgcta gaacggaagt tggatgatgt atctgagcag
     1141 atagatttgc taatacaaga ggattcgtaa tgaccttttg acctgccccc gggagtttca
     1201 ggatttccgt gtttaaagcg gcgcctagga aaagcggggc aattcgtcga ccgtattgtt
     1261 aaactcgact caggtaagct ggaagagtat atttgaccgt ttttggtaat ctcccggtaa
     1321 ttccctcatt tttaaggaaa gctaaaagta gaggctaagc cacgagggcc aatttttgct
     1381 tcggggtgat accgccgagg gccatgttcg ggcgttcgtg attgtaggtc cagagccaat
     1441 cggtggcaaa ttcctggatc tcatcagtcg attcaaacag gtaatgcgcc agccagtcat
     1501 agcgaacggt cctgttgtag cgctcgatat aggtgggggg tatccgggat tttgcgtcaa
     1561 cgtaaaattt ttgtcacgat ggagacgccg tcgtcatgcc tgcttccgtc cttcctgcca
     1621 tcctgcaaaa cctgcgtttg cctgtcattg ccgcgccgat gttcatcgcc agcaacccga
     1681 cactggtggc agcccagtgc aaggccggca tcgtcggttc ttttcccgcg ctcaatgctc
     1741 gcccggctga acagctggat gtctggctga cgcaattgca gacggaactg gcggcctgca
     1801 aggcgacgaa tcccgacgcc atgatcggcc cgatcgcggt caaccagatc ctgcatcgat
     1861 ccaatgagcg cctggcccgt gacgtggacg tctgcgtcaa gcatcgcata cccatcatca
     1921 tttcgagttt gcgcgcgccg tcgcaggcga tgctggacac cattcacagt tatggcggca
     1981 tcatactgca cgatgtgatt tcgatccggc atgcgcaaaa agcactggag gccggcgtcg
     2041 atggcctgat tctggtagcc gccggcgccg gcggtcatgc cggtgcgctg tcgccgttcg
     2101 cgctggtggg tgaggtgcgc agattcttca gtgggccgct gatcctgtcg ggcgcgatcg
     2161 ccaccggcga cgcgatcctg tcggcgcaag ccatgggcgc cgatctggct tacatcggct
     2221 cgcgctggct ggcgacccgg gaagccaatg tcagcgcacc atatcgtcag gccatcatcg
     2281 aatccactgc caccgacgtg gtgtatacca acctgtttac cggcgtgcat ggcaattacc
     2341 tgaagaaatc tatcatcaat gccggtcttg atcccgacaa tctgcccgca gcggacaaga
     2401 gcaggatgaa ttttggttcc ggcggcgaag ccaggacctg gcgcgacatc tggggtgctg
     2461 gtcaggctgt gggcctgatg gacgacgtgc cgcgcgtggc ggagatggtg acgcgcctgg
     2521 aatgcgaata tgcggcagcg cggcaacgcc tgtcgctctg agaacgtcac acgttctcag
     2581 acaaagccgc tattttccac atgaatctgt aacttctttc tcacttgcct gtcgtatgat
     2641 ttccaagcat tcatccgtga ggactttatg accagttttc ttcgcaccag aaaagaccgc
     2701 catcccaaac cggaaaacaa ggacaggctg ctgcgcacgc ataccccatc cgacggcaac
     2761 catacaggcc ggacggaaaa caccctgcgc ggcaacagta accccgatga agtcacccag
     2821 gagaacagcg aaacaccgcg cggaaccgac catgatccgt cgtcgcattg atccctgttg
     2881 aatacaccgc cgcctatccg ggtctgccgt gatgccacat cattccagcc acctggcgcg
     2941 gctcgccgcc agcgcggccg cgcagcacag cacaccgatg aacaccaacg ccgccggcaa
     3001 gccgaaacca tgcgccagaa agccgatcac cggcggaccg gccagactgc cgctgtaacc
     3061 gagcgtggcc acgccggcca gtgcgaccgt gccatgccgt cctgctgcgc tgaccacgaa
     3121 cgggaacgct accgccacac cggcgccggc catggcgaaa ccaaggatca ccatcggtat
     3181 gttcactgcc gccacggcga cgaacacact gacgcttgcc agcagcgcgc cccagcc
//
LOCUS       DASRDH010000020         3675 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_676240,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000020 DASRDH010000000
VERSION     DASRDH010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 3675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3675
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676240"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..1045)
                     /gene="waaC"
                     /locus_tag="VFA14_00515"
     CDS             complement(38..1045)
                     /gene="waaC"
                     /locus_tag="VFA14_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005665333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HZS79904.1"
                     /translation="MKILIVRVSSLGDVVHNMPMVADIRHHYPDAHIDWVVEEAYADL
                     VRLNPHVREIIPIALRRWRKSLVSTATRTEIAGFYRRLRREAYDFVFDTQGLLKTGAV
                     MRMARLMPAGMRAGLANATEGSGYEALSRIFHDRSIPADLHMHAVTRARTVAAATLGY
                     TLDDAVDFALQEPASMPTPAWLSRQAYAVFFHGTARAAKQWDRNNWVVVAQALAERGL
                     PVWLPWGSAAEKQAAEDLAARMPQATVLPALSLMEAVTLARHAALVVGLDTGLTHIAA
                     AYCRPTVELYCDSPRWKTEGNWSPRIANLGECGAPPDAVQVLLAIDRVLSAPLTQDDE
                     A"
     gene            complement(1046..2416)
                     /locus_tag="VFA14_00520"
     CDS             complement(1046..2416)
                     /locus_tag="VFA14_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008117345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HZS79905.1"
                     /translation="MISSSIFKAYDIRGIAGQTLDADVAHQIGRVFGSAASSRGQTAV
                     VTGRDARLSGAGLGTALVAGLQATGIDVIDLGVVTTPMVYFGTHVLGTQSGIMVTGSH
                     NPPDYNGFKMVLAGEAIHGDAIQALYRAILKQEFAAGAGNYRTYDIRREYMARITSDA
                     RLARPMKIAIDCGNGVAGAFAGELYRALGCEVTELFCEVDATFPNHHPDPAHPENLQD
                     LIRCLQESDNEIGLAFDGDGDRLGVVTRDGQIIYPDRQLMLFAADVLTRHPGREILYD
                     VKCTQHLAPWISAHGGQPLMWKTGHSLIKAKLRETGAPLGGEMSGHLFFKDRWYGFDD
                     ALYAGARLLELMSREADPSAALNALPQSTSTPELQWQLEEGENFALIDQLQQEASFPD
                     AERIITIDGLRVEYAHGFGLMRSSNTTPVVVLRFEAQTPQALARIQNEFRRVILTARP
                     DAALPF"
     gene            complement(2437..3237)
                     /locus_tag="VFA14_00525"
     CDS             complement(2437..3237)
                     /locus_tag="VFA14_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012761568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HZS79906.1"
                     /translation="MYRRQFIQFFASLGAAAGFAFAPALAQDKPIRIGVTGGVHAEIM
                     EQVKKLAEKEGLKLRIVEFSDYIQPNAALAAGDLDANSYQHQPYLDAQIKDRGYKLVS
                     IGSTITFPMAVYSKKIKSLEELQNGARIGVPNDPSNGGRALLLLQTQGLIRLKPAAGL
                     TATPLDIVDNPKKLRIVELDAAQLPRALHDFDAAVINDNFANSAGLSPTRDGIAMEAI
                     DGPYVNIIAVRAEDRNKPWVTRLLKVYRSPEIKKFVQQRYGDAAMTSW"
     gene            complement(3267..>3675)
                     /locus_tag="VFA14_00530"
     CDS             complement(3267..>3675)
                     /locus_tag="VFA14_00530"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016345956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HZS79907.1"
                     /translation="VGTSIGTAAAIVPLTIAAAPLIARLVETALREVDHGLVEAAQAM
                     GATTWQIIYKVLVPEAFAGIVAGLTITFVSLVGYSAMAGAIGGGGLGDLGIRYGYQRF
                     LPEVMLIVVLILIVFVQLVQTLGDILVRRLSHR"
BASE COUNT          725 a         1103 c         1099 g          748 t
ORIGIN      
        1 gacgtcgatt tccatgattt gcgatttcat cccggtatta tgcctcgtca tcctgagtca
       61 gtggcgcgga taatacccga tcgatggcta gcaggacttg cacggcatcg ggaggcgcgc
      121 cgcactcgcc gaggttggcg atgcgcggcg accagttgcc ttcggtcttc cagcgcggcg
      181 agtcacaata cagctcgacg gtgggacggc aatatgccgc cgcgatatgc gtcaggccgg
      241 tatcgagtcc caccaccagc gcggcatgcc gcgccagcgt tacggcttcc atcaatgaca
      301 gcgccggcaa cacggtcgct tgcggcatgc gcgcggcgag gtcttccgct gcctgttttt
      361 ccgctgcgct accccacggc agccagaccg gcagcccccg ttcggccagc gcctgcgcca
      421 cgacaaccca gttgttgcga tcccattgct tggctgcgcg cgccgtgccg tggaaaaaca
      481 cggcataggc ttgccgcgac agccaggccg gggtcggcat cgatgctggt tcctgcaagg
      541 caaaatccac cgcatcgtcc agcgtatagc cgagtgtggc agcagcaacc gtccgtgcgc
      601 gtgtcaccgc gtgcatatgc aaatctgccg gaatgctccg gtcatgaaaa atcctggaaa
      661 gcgcttcgta acccgagcct tcggtcgcat tggccagtcc ggcccgcatc cctgctggca
      721 tcagacgcgc catgcgcatc acggcgccag tcttcagcaa gccctgggta tcgaaaacaa
      781 aatcgtaagc ttcgcgtcgc aagcggcgat aaaaaccggc gatttctgtg cgcgtcgcgg
      841 tactgaccag gctcttgcgc cagcgccgca aagcaatcgg gatgatctcg cgcacatggg
      901 gattgagccg caccagatcg gcataagcct cttccaccac ccagtcgata tgggcgtccg
      961 gataatggtg ccggatgtcg gcgaccattg gcatgttgtg taccacgtcc cccagggatg
     1021 agacgcggac gatcaggatt ttcatttaaa acggcaaggc tgcatccggg cgtgcagtca
     1081 ggatcacacg cctgaattca ttctgaatgc gtgccagcgc ctgcggcgtc tgcgcttcga
     1141 agcgcagcac caccaccggc gtggtattgg aagaacgcat caggccaaaa ccgtgggcgt
     1201 actccacgcg taaaccgtcg atcgtgatga tccgttcggc atcggggaag cttgcttctt
     1261 gttgcaattg atcgatcagc gcgaagttct cgccttcttc gagctgccat tgcagctcgg
     1321 gcgtgctggt cgattgcggc aaggcattca gcgcagccga aggatcagcc tcgcggctca
     1381 tgagttccag caagcgcgct ccggcataca aagcatcatc gaaaccatac cagcgatctt
     1441 tgaagaacag gtggccgctc atctcaccgc ccagcggcgc gccggtttca cgcagtttgg
     1501 ctttgatcag cgaatggccg gttttccaca tcagtggttg accgccatgt gcgctgatcc
     1561 acggcgccag atgctgggtg catttgacgt cgtacagaat ttcgcgaccg ggatggcggg
     1621 taagcacatc ggcggcaaac agcatcagtt gccggtcggg ataaatgatc tggccatccc
     1681 tggtcaccac gccaagccgg tcgccatcgc catcgaatgc caggccgatt tcgttgtcgc
     1741 tctcctggag acagcggatc agatcctgca aattttccgg atgggccggg tcgggatgat
     1801 gatttggaaa agtagcatcg acttcgcaga acaattccgt cacttcacag ccgagcgccc
     1861 ggtacagctc accggcgaac gcgccggcca cgccgttacc gcaatcgatg gcgatcttca
     1921 tcgggcgcgc cagtctggca tcgctggtga tgcgcgccat atattccctg cgaatgtcgt
     1981 aagtacggta gttgccggcg ccagcggcaa attcttgctt caggatggcc cgatacagcg
     2041 cctggatggc atcgccatgg attgcctcgc cggccagcac catcttgaaa ccgttgtagt
     2101 cgggcggatt gtgactgccg gtgaccatga tgccggattg cgtgccaagt acatgtgtgc
     2161 cgaaatacac catgggcgtc gtgacgacgc cgagatcgat gacatcgatg ccggtggcct
     2221 gtagacctgc aaccagcgcc gtgcccaacc cggcgcctga caggcgagcg tcacgaccgg
     2281 tcacgaccgc agtctggccc ctgctcgatg cagcactacc gaagacgcgc ccgatctgat
     2341 gggcgacatc ggcgtccagg gtctgaccag caatgccgcg gatgtcgtag gctttgaaga
     2401 tggaagagga aatcatgtct tttgccgtga ttcagattac caggaagtca tcgctgcgtc
     2461 tccgtaccgc tgctgcacga attttttgat ttccgggctg cggtaaacct tgagcagtct
     2521 ggtgacccag ggcttgttcc ggtcttcggc gcgcacggcg atgatgttga cgtagggacc
     2581 atcgatagct tccatggcga tgccgtccct ggttggcgac aggccggctg agttggcgaa
     2641 gttgtcattg atgacggcag catcgaagtc atgcagcgca cgcggcagtt gcgctgcatc
     2701 cagttcgacg atcctgagct ttttcggatt atcgacgatg tccagcggcg tcgctgtcag
     2761 gccggcagca ggcttgagcc tgatcagccc ctgggtttgc agcaacagca aggcgcggcc
     2821 gccgttgctc ggatcattcg gcacgccgat gcgtgcgcca ttctgcaact cctcgagtga
     2881 cttgatcttc ttcgagtaga ccgccatcgg gaaagtgatg gtggagccga tactgaccag
     2941 cttgtagccg cggtccttga tttgcgcatc gagatacggc tggtgctgat aactgttggc
     3001 gtccaggtcg ccggcagcca gcgcggcgtt gggctgtata tagtcgctga attcgacgat
     3061 gcgcagcttc aggccctctt tttcagccag ttttttcact tgctccatga tttcagcatg
     3121 cacaccgccg gtgacgccga tcctgatagg cttgtcttgc gctagggctg gagcaaaggc
     3181 gaagcctgct gctgcgccca ggctggcgaa aaactggatg aattgacgac gatacataat
     3241 ctccctttca tttttcaaag atggattcaa cggtggctca gacgccgcac caggatatca
     3301 cccagtgttt gcaccagttg cacaaagacg atcaggatca gcaccacgat cagcatgact
     3361 tccggcagga agcgctgata gccgtagcgg atgccgagat cgcccaggcc gccgccgccg
     3421 attgcgcccg ccatggcgga atagccgacc agactgacga aggtgatcgt cagccctgcg
     3481 acgatacctg caaaggcttc tggcaccaat accttataaa tgatttgcca ggtggtagcg
     3541 cccatggcct gggcggcttc gaccaggcca tgatcgactt cgcgcagcgc ggtttcgacc
     3601 aggcgtgcga tgagcggcgc ggcggcaatc gtcagcggta cgatcgcggc agccgtgccg
     3661 atcgatgtgc cgacg
//
LOCUS       DASRDH010000021         2333 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_459974,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000021 DASRDH010000000
VERSION     DASRDH010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459974"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="VFA14_00535"
     CDS             complement(<1..552)
                     /locus_tag="VFA14_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020749240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZS79908.1"
                     /translation="MLSTSNITMQFGSKPLFENISVKFGEGNRYGLIGANGCGKSTFM
                     KILGGDLEASSGNVSLDNDERLGKLRQDQFAYEEMRVLDVVMMGYTEMWAAMSERDAI
                     YANPDATDDDYMTAAGLEARFAEYDGYTAEARAGELLLGVGVPIAQHRGPMSNVAPGW
                     KLRVLLAQALFSNPDILLLDEPTN"
     gene            complement(800..1201)
                     /locus_tag="VFA14_00540"
     CDS             complement(800..1201)
                     /locus_tag="VFA14_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008328027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZS79909.1"
                     /translation="MTDDQYFLVVDDFATMRRIVTGLLKELEFTKIAEAEDGSTAMKM
                     LESGTIPVTFVLTDWDMPVMNGLELIKKIRATSSLAHLPILMLTAEAKRENVIEAAEA
                     GVDGYIVKPFNAATLKKKIEKILERRANAAH"
     gene            complement(1417..1602)
                     /locus_tag="VFA14_00545"
     CDS             complement(1417..1602)
                     /locus_tag="VFA14_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017233791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HZS79910.1"
                     /translation="MKLTHYAGQTLRLMVGLPEYDAYVAHMRTTHPEAEVMSYEAFFR
                     ERQEARYGGKGRVGRCC"
     gene            complement(1666..>2333)
                     /locus_tag="VFA14_00550"
     CDS             complement(1666..>2333)
                     /locus_tag="VFA14_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013233956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbon starvation CstA 5TM domain-containing
                     protein"
                     /protein_id="HZS79911.1"
                     /translation="FAILFEALFILTAVDAGTRAGRFMLQDLLGSFIPAMKRTESWLA
                     SLIATGLCVAGWGYFLYQGVVDPLGGINTLWPLFGISNQMLAAIALILATCVLFRMKR
                     DRFAWVTIIPTIWLLFCTLTAGWQKVFDDNPRIGFLAHAIKYQAALSEGRLLEPARSI
                     EQMRQVIFNDYVDAGLTVVFILLLISMLLFGIRTILQARTSQDPSVQESTYENMPAST
                     LAK"
BASE COUNT          515 a          649 c          621 g          548 t
ORIGIN      
        1 attggtcggt tcgtcgagca gcaggatatc cggattcgag aacagcgcct gcgccagcag
       61 cacgcgtagt ttccagcccg gtgcgacatt gctcatcggg ccgcgatgct gcgcgattgg
      121 cacgcccacg cccaacagca attcgccggc gcgcgcttcg gcggtatagc catcgtattc
      181 ggcgaatctg gcttctagcc cggctgctgt catgtaatcg tcgtcggtag cgtcgggatt
      241 ggcataaatc gcgtcgcgtt cggacatcgc tgcccacatt tcggtgtagc ccatcatcac
      301 gacatccagt acgcgcattt cttcgtaggc gaactgatcc tggcgcagtt tgcccaggcg
      361 ttcgtcatta tccaggctga cgttgccaga ggaagcttcc agatcgccgc caagaatttt
      421 catgaaagtt gacttgccgc agccgttggc gccgatcaag ccatagcgat tgccttcgcc
      481 gaatttgacg gaaatatttt caaacagcgg cttggagccg aactgcatgg tgatgttaga
      541 ggtcgatagc actgaaaaaa cctttgttac atagttgctg aaaatattgc gggcgacagc
      601 ctgattccag gtacgcgcgc ataaaccacg ccgtcggcct ggttggggga agttgcgata
      661 acagtgtttc gcgagtgtgc tgccttcagt acggacaggt gcccgtcaga ctgcactgta
      721 gcaccgacgg atcggtgctt ccggcttgcg gaatacgatg attttcttgc ccttctcggc
      781 tgatgggatg ccgtcctgct caatgggccg cgtttgcccg tcgctccaga atcttttcta
      841 tttttttctt tagtgtcgct gcgttgaacg gtttgacgat gtagccatcg acgccggcct
      901 ccgctgcctc gatgacgttt tcccgcttcg cttccgcggt aagcatcagt atcggcaggt
      961 gcgccagtga tgacgtcgcg cggattttct tgatcaactc caggccattc atcaccggca
     1021 tgtcccagtc ggtcaggaca aacgtaacgg gtatcgtgcc ggactccagc atcttcatcg
     1081 cagtagagcc atcttctgcc tcggctatct tggtaaactc gagttctttg agcaaaccgg
     1141 taacgatacg cctcattgtg gcaaaatcgt ccactactag gaaatattga tcatctgtca
     1201 tttgagtaat tgatagcttg caagtcaaca gaaggagggt ttgtctggcg tctttcgatt
     1261 tctgccagtg taaggcattt tgtacgttcc tcctacttta cactatctgc gcggcattct
     1321 ctatcgatga aacggtggcg actggccctc tttgcacgta gaagcagata gacagcaacc
     1381 aaaggtaaaa aaaatttctg tagagaaata aaccgctcag cagcaccgtc ccacccgtcc
     1441 cttgccgcca taacgcgctt cctgacgctc acggaagaag gcttcataac tcatgacttc
     1501 ggcttcaggg tgggtcgtcc gcatgtgcgc gacgtaagca tcgtattcag gtaaacccac
     1561 catcaaacgc agggtttggc cggcatagtg tgtcaatttc atgcattcct gcaaaatacg
     1621 tggtgtgtcc attgcgtgtt ttcccgttct cgtgatggtg ccttgtcatt tggccaatgt
     1681 gctggccggc atgttttcgt aagtagattc ctggacgctg ggatcctggg atgtccgcgc
     1741 ctgcaatatg gtgcggatcc cgaacaacag catgctgatc agcaacagta tgaacaccac
     1801 tgtcagaccg gcatctacgt aatcgttgaa aatgacctgc cgcatctgtt cgatcgacct
     1861 ggccggctcc agcagcctgc cttcggagag tgcggcctga tacttgatcg catgcgccag
     1921 gaagccgatg cgcggattgt cgtcgaacac tttctgccag ccagccgtca gtgtgcagaa
     1981 caataaccag atagtcggga tgatcgtcac ccaggcaaag cgatcacgtt tcatgcgaaa
     2041 cagcacacag gtcgccagaa tcagcgcaat ggcagccagc atctggttgg agatgccaaa
     2101 cagcggccac aaggtattga taccgcccag cggatcaacc acaccctgat acagaaagta
     2161 accccagccg gcgacacaca agccggtcgc aatcaggctg gcaagccacg attcggtacg
     2221 cttcatggcc ggtatgaagc tccccagcag atcctgcaac atgaaacggc cggcgcgtgt
     2281 gcccgcatcg acggcagtca ggatgaacaa ggcttcaaac aggatcgcga agt
//
LOCUS       DASRDH010000022         3143 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1131642,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000022 DASRDH010000000
VERSION     DASRDH010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 3143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3143
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1131642"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1046
                     /locus_tag="VFA14_00555"
     CDS             <1..1046
                     /locus_tag="VFA14_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015603558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HZS79912.1"
                     /translation="FAPFGLSGVAMAVSAGGVIFAYLGLTPIISVASEVRNPQRTIPF
                     ALILSILLSTVIYVLLQLVFLGSVPAGMLADGWAAVSKEMALPYRDMAMVLGVGWLAY
                     LVVADAIVSPVGCGNIFMSATPRVVYGWAQTGTFFKVFTRVEETSGIPRPALWLTFGL
                     SIFWTLPFPSWEALINVVSVALVLSYAVAPISVAALRRSAPVMVRPFRVTAVSVIGPL
                     AFITAALIVYWSGWKTVSWLLGLQILMFALYLLCARIVPTQRVSLAQQVRSSAWLIGL
                     YVALIGLSSIGTYGGAGILAHPFDSLAIAACALLIYYWGAVTGLPSQLLDLESDDSES
                     EEQLSSVPAVGIA"
     gene            1178..1819
                     /locus_tag="VFA14_00560"
     CDS             1178..1819
                     /locus_tag="VFA14_00560"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007881896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HZS79913.1"
                     /translation="MPKPYPHPLIAREGWPFLGIAVVVAIVVTVFGGAWSIPFWVIAL
                     FVLQFFRDPPRTIPQDPNVVLSPADGRIVMVAQAHDPYANREALKISVFMNVFNVHSN
                     RAPVDGKIESVQYFPGRFVNADLDKASLENERNALVVTAANGQSVTCVQVAGLIARRI
                     LCHVKAGERLARGQRYGFIRFGSRVDVYLPLTATPKVGVGEKVSATETILAEF"
     gene            1854..2735
                     /gene="pssA"
                     /locus_tag="VFA14_00565"
     CDS             1854..2735
                     /gene="pssA"
                     /locus_tag="VFA14_00565"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007881897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HZS79914.1"
                     /translation="MTTFRKRKNKDIPFPKSLRSHKTPIYPLQLEDEDDTLIIRPRRS
                     RGIYLLPNAFTTGALFCGFYAIVMAMNLKFDHASIAIFVAMVLDGLDGRVAYMTNTQS
                     EFGAQYDSLSDMVSFGAAPALVVYEWSLRGMGKLGWLAAFVYCVGAALRLARFNTNIA
                     VIDRRYFQGLPSPAAAALVAGFVWLMDDLGFAGADLSWVSWIITLYAGITMVSNVPFY
                     SFKDINFRKPVPFIAALLVVLMLVAISSAPSKVLFGLFVLYGVSGYFLYVWRKFKGKP
                     VSSAQTGREEKKQVHTP"
     gene            2877..>3143
                     /locus_tag="VFA14_00570"
     CDS             2877..>3143
                     /locus_tag="VFA14_00570"
                     /inference="COORDINATES: protein motif:HMM:NF024573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZS79915.1"
                     /translation="MKLRRLCICNFRSIIDADVEVHDYLMIVGANNAGKSNFLAVLRA
                     FYEDIKWSDEDVPKAGNNGGESWVELTYALTDAEWDGLADKYKMN"
BASE COUNT          578 a          826 c          968 g          771 t
ORIGIN      
        1 agtttgctcc tttcggcttg tctggcgtgg ccatggctgt gtcggctggc ggcgtcatct
       61 ttgcctacct gggtctgacg cccatcattt cggtggccag cgaagtgagg aacccgcagc
      121 gcaccattcc cttcgcgctg attttgtcga tactgctgtc cacggtcatt tatgtcttgc
      181 tgcaactggt gtttctgggg agcgtgcctg ccgggatgct ggcagatggt tgggcagctg
      241 tgtcgaaaga gatggcattg ccctatcggg acatggccat ggtgctggga gtgggatggc
      301 tggcttatct ggtggtggca gatgcgatcg tttcgccagt cggctgtggc aatatcttca
      361 tgagcgcgac gccgcgcgtg gtctatggct gggcgcaaac gggtactttc ttcaaggtgt
      421 ttacacgtgt ggaggaaaca tcgggcatcc cgcggccggc gctgtggctg acttttggcc
      481 tgtcgatctt ctggacgctg ccgtttccgt cgtgggaggc gctgatcaat gtcgtgtctg
      541 tcgcgctggt cctgagctat gccgtggcgc cgatctcggt ggcggccttg cgccgtagcg
      601 cgcccgtcat ggtgcgcccg tttcgggtaa cggcagtgag cgtgatcggt ccgctggctt
      661 ttatcacggc tgcattgatc gtgtattggt cggggtggaa gacggtgtcg tggttgctcg
      721 ggttgcagat cctgatgttc gcgctctatc tgttgtgcgc ccggatcgtc cccacgcagc
      781 gcgtgagcct ggcgcagcag gtacgctcgt ctgcctggct gattggcctg tatgtcgccc
      841 tcattggctt gtccagcatc ggcacttatg gcggggcagg catcctggcg catccgttcg
      901 actccctggc gatcgctgct tgcgcactgc tcatctatta ctggggggcg gtcaccggtt
      961 tgccgtcaca gctactcgac ctggagtcgg atgactcgga aagcgaggag cagctatcct
     1021 cagtgccggc agtcggtatt gcgtgatgat gatcggcaat gagatatatc caagggaagt
     1081 cacgagataa ctccgggaga aggtcggaaa tcgggaagaa acgggatccg ctctggattc
     1141 gcgatagaat acgcgcttat caaaaatcgg agcagcattg cctaagcctt atccacatcc
     1201 cctcattgcc cgcgaaggct ggcctttcct gggtatcgcc gtcgtcgtcg ccatcgttgt
     1261 cactgtgttc gggggcgcct ggtcgattcc attctgggtc atcgccctgt tcgtattaca
     1321 gtttttccga gacccgccgc gcaccattcc gcaagacccg aatgtcgtgc tgtcgccggc
     1381 cgatggccgc attgtcatgg tcgctcaggc ccatgacccg tatgccaacc gtgaagcgct
     1441 gaaaatcagc gtgttcatga acgttttcaa tgttcattcc aaccgcgcgc cggtagatgg
     1501 caaaatcgaa agcgtgcaat atttccccgg cagattcgtc aatgccgatc tggacaaggc
     1561 gtctctggaa aatgaacgca atgccctggt agtgactgcc gccaatggtc agagtgtgac
     1621 ctgcgtgcag gtggccggtc tgattgcgcg ccgcattctg tgccatgtca aggcaggaga
     1681 gaggctggcg cgcggtcagc gctatggctt tatccgtttt ggctcgcgcg ttgacgtgta
     1741 tctgccgctg acggcgacgc cgaaggtggg cgtgggtgaa aaagtttcgg caacggaaac
     1801 gatcctggct gaattctgat cactgcatga ccagtttatt gaaatgaccc accatgacaa
     1861 ctttccgcaa gcgtaaaaac aaggacatcc cttttccgaa atccctgcgc agtcataaaa
     1921 cgccgatata tccgctccag ctggaagatg aggacgatac actcatcatt cgtccacgtc
     1981 gttcccgcgg catttatctg ttgccaaacg cttttaccac gggggccttg ttctgcggtt
     2041 tttacgccat cgtcatggcg atgaatctga agttcgatca tgcgtcgatt gccatttttg
     2101 tcgccatggt actcgatggc ctggacggtc gcgtggcgta catgaccaac acccagagcg
     2161 aattcggcgc gcagtacgac agcttgtccg atatggtgtc gttcggtgcg gcgccggcgc
     2221 tggtggtcta cgaatggtcg ctgcgcggca tgggcaagct gggctggctg gcggcgttcg
     2281 tatattgcgt tggcgccgcg ttgcgtctgg cgcgtttcaa taccaatatc gcagtaatcg
     2341 acaggcgtta tttccagggc ttgcccagcc cggcggcggc ggcattggtc gccggtttcg
     2401 tctggctgat ggatgacctg ggttttgccg gcgcggattt gagctgggta tcctggatca
     2461 tcacgctgta cgccggcatc accatggtga gcaatgtgcc gttctacagc ttcaaggaca
     2521 tcaatttccg caagccggtg ccgttcatcg cagccctgct ggtggtgctg atgctggtgg
     2581 cgatctccag tgccccttcg aaggtgctgt tcggcctgtt cgtgctgtat ggcgtgtcgg
     2641 gttactttct ctatgtctgg cgcaaattca aaggcaagcc ggtcagcagc gcgcagaccg
     2701 ggcgcgagga gaagaaacag gtgcatacgc catgacatta actgcattgc ccgtttcgtt
     2761 gactgaaccg gatggtaatg ggcggcgcgc tttgtcagcg ggttacaggt tgaacgccaa
     2821 ggtgaaaagc gtggtaagcg ctatcgaatc aataaatctc tctcggggga atgatcatga
     2881 agcttcgccg cctgtgcatt tgcaacttcc gctcaatcat cgacgccgat gtcgaagttc
     2941 atgactatct gatgatcgtt ggagccaaca atgctgggaa aagcaatttt ctggcggtgc
     3001 tacgagcttt ttacgaggac atcaaatggt cagacgaaga tgtgccgaaa gcaggaaata
     3061 acggcggcga atcttgggta gaactgactt atgcgctgac cgatgccgaa tgggatggtt
     3121 tggcggataa gtacaagatg aat
//
LOCUS       DASRDH010000023         3986 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_329437,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000023 DASRDH010000000
VERSION     DASRDH010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 3986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3986
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329437"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1138
                     /gene="pcnB"
                     /locus_tag="VFA14_00575"
     CDS             <1..1138
                     /gene="pcnB"
                     /locus_tag="VFA14_00575"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HZS79916.1"
                     /translation="QLFRRAFIIGRRFQIVHVMFGQELIEVTTFRSSSANSAPRDEHG
                     RMLSDNTFGEQHEDAMRRDFTINAMYYDPASQSVFDYHGGIADIHARTLRIIGMPKER
                     YREDPIRMLRVARFAAKLDFSIDAATSAPIAIMAPLINNVPVARVFDEMLKLLMSGHA
                     LACLQQLRAQGLHHGLLPLLDVVLEQPLGEKFITLALANTDTRIKQGKPVSPGFLFAS
                     LLWHQVLEKWRAYQAAGEYPIPALHLATDDVLNAQTEKLALQRKITSDMRDIWAMQPR
                     FERRSGKAPYKLLEHLRLRAGFDFLLLRCASGEADAELGTWWTSFIGADGPEREVLLA
                     LKPNAAADANAGAGAKSARKRRRRGGRKRSKSSNTSQAAGDASA"
     gene            1135..1635
                     /gene="folK"
                     /locus_tag="VFA14_00580"
     CDS             1135..1635
                     /gene="folK"
                     /locus_tag="VFA14_00580"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010401665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HZS79917.1"
                     /translation="MSAPVIAHIGIGSNLGDARATAQDALRRLARLPRTTLSAQSRLY
                     RTAPVDAGGNDYVNAVARLATALTPHALLVALQDIEQAHGRQRPYRNAPRTLDLDILL
                     YGELTISDDTLTVPHPRMTQRAFVLIPLLEITPDIIIPGIGAALTLRAQVADQAIEAI
                     AVLDET"
     gene            complement(1675..2328)
                     /locus_tag="VFA14_00585"
     CDS             complement(1675..2328)
                     /locus_tag="VFA14_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HZS79918.1"
                     /translation="MLLATSVWAEDVASESATEGVLLATHSAHGRIPVKSWKTLRDAR
                     IVKQNLDYSCGAASLATLLDSYYGQNVTEEALLKAMDKGEGRASFEDMARALPQFGFR
                     AQGFAASWEQLERLKMPVVVYVKHRRDDHFSVLRGISGDTVWLADPSVGNRTYSREQF
                     LAMWQTRNDTDDGLAGKFLAVLPADAAKALVSEDFFTRTPRRQSANATAQLAFRRQP"
     gene            complement(2566..2895)
                     /locus_tag="VFA14_00590"
     CDS             complement(2566..2895)
                     /locus_tag="VFA14_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS79919.1"
                     /translation="MKVYMKKFKRATLILVTASLLSVQVQAYANQARQENVQAPPVAA
                     FAQTDIDTMFVQTDQPMQLAVLSQQEMKETEGAFLPALLAIVSIISAALNASAAGVIL
                     AANIPKL"
     gene            complement(3015..3884)
                     /locus_tag="VFA14_00595"
     CDS             complement(3015..3884)
                     /locus_tag="VFA14_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS79920.1"
                     /translation="MRFILGVLLVSLSHLAWADLPLTVEDLITDKGKLKLEASLSYAN
                     ADRHGISATDPVTIQTGPASFITLPTRIGESVGNSDTTVATLGLRYGLTAKAEIYARL
                     SGLASSQRSSGLDGSRTSSESRFADAWAGVNYQFKQDNDTPAVLGFAEIALHEKHRVS
                     SSSFKSAMLGLTIYKAIDPVVFSLTSAYRFNHERADGAQHYQPGSLLLLSPGLAFAIN
                     DRVTTTTGVQWTRHAADRFDGQAQGIARTATDLLLGVGYGLAKGNTLSTTLKLNTSGR
                     DGAEIRVNWLYML"
BASE COUNT          858 a         1218 c         1103 g          807 t
ORIGIN      
        1 acaattgttc cgacgcgcct tcatcattgg ccgccgcttc cagatcgtcc acgtgatgtt
       61 cggccaggaa ctaatcgaag tcaccacctt ccgcagcagt tccgccaaca gcgcccccag
      121 ggatgaacat ggccgcatgc tgtccgacaa caccttcggc gaacagcacg aagacgccat
      181 gcggcgcgat ttcaccatca atgccatgta ctacgatccg gcgtcgcaaa gtgtattcga
      241 ctatcacggc ggcatcgccg atatccacgc caggacgttg cgcatcatcg gtatgccaaa
      301 agaacgctat cgcgaagatc cgatacggat gctgcgcgtg gcgcgctttg ccgccaagct
      361 cgatttttcc atcgacgccg ccaccagcgc gccgatcgcc atcatggcgc cgctgatcaa
      421 caatgttccc gtcgcgcgcg tgttcgacga aatgctgaag ctgctcatga gcggacacgc
      481 cctcgcctgc ctgcagcaac tgcgcgcaca aggtctgcat cacggtttgc tgccgctgct
      541 cgacgtcgtg ctggaacaac cgctgggcga aaaattcatc acactcgcgc tggctaatac
      601 cgatacccgc atcaagcagg gcaaaccggt ttcgccgggc tttctgttcg cctcgctgct
      661 atggcatcaa gtgctggaaa aatggcgcgc ctatcaggcg gccggtgaat atccgattcc
      721 cgcgctgcat ctggcgaccg atgatgtact caacgcacag accgaaaaac tggcgctaca
      781 acgaaagatc acttccgaca tgcgtgatat ctgggccatg cagccgcgct tcgaacgccg
      841 ttccggcaaa gcgccctaca agctgttgga gcatctgcgc ctgcgcgccg gtttcgactt
      901 cctgctgctg cgctgcgcct ccggtgaagc tgatgccgaa ctcggcacat ggtggacctc
      961 cttcatcggc gccgacggcc ctgagcgcga agtcctgctt gcgctcaaac ccaacgccgc
     1021 cgcagacgcg aatgcgggtg ccggcgccaa atccgccagg aagcgcagac gccgcggtgg
     1081 tcgcaagcgc agcaagagca gcaacaccag tcaagcagcc ggcgatgcat cagcatgagc
     1141 gcgccggtca tcgcccacat cggtatcggc agcaatctgg gcgatgcccg cgccacggca
     1201 caggatgcct tgcgccggct ggcacggttg ccgcgcacca cgctgagcgc gcagtcccgt
     1261 ctataccgca cggcaccagt cgatgccggc ggcaatgatt atgtcaatgc cgtagcccgg
     1321 ctcgccaccg cgctgacgcc acatgccttg ctagtcgcgc tgcaagacat cgaacaggcg
     1381 catggacgcc agcgccctta ccggaacgcg ccgcgcacac tcgatctgga tatcctactg
     1441 tacggtgagc tgacgatcag cgacgacacg ctgacggtgc cgcatccacg catgacacaa
     1501 cgcgcgttcg tcctgattcc cctgctggaa attacgcctg acatcatcat tcccggcatc
     1561 ggcgccgcgc tgacgctacg cgctcaggtg gccgaccaag ccatcgaagc gatcgctgtc
     1621 ctcgacgaaa cctgatccgg cacattgctc acaaccggag cgcctacaga aatatcaggg
     1681 ttgcctccgg aatgccagtt gagccgtcgc attggcgctt tgccggcgcg gcgttctggt
     1741 gaagaagtcc tctgagacca gcgccttggc ggcatcagcg ggcaataccg ccaaaaactt
     1801 gcccgccaga ccgtcatcgg tatcgttgcg tgtctgccac atagccagaa actgttcacg
     1861 gctgtaagtg cgattgccca cgctggggtc agccagccac accgtgtcac cgctgatacc
     1921 gcgcaacacc gagaaatgat catccctgcg gtgcttcaca tagaccacga ccggcatctt
     1981 caggcgctca agctgttccc agctcgcggc gaagccctgc gcccgaaaac caaactgcgg
     2041 cagggcacgc gccatgtcct cgaaactggc ccgcccttct cccttgtcca tggctttgag
     2101 caaagcctct tcggtgacgt tttggccata gtagctgtcg agcaacgtcg ccagagacgc
     2161 tgcaccacag gaatagtcca gattctgctt gacgatgcgc gcatcgcgca gtgtcttcca
     2221 gctcttgacg ggaatgcgac cgtgagcgga gtgggtcgcc agtaataccc cttcggtggc
     2281 tgattcgctg gcgacatcct cagcccatac agacgtggcc aacagcacag caaaacagca
     2341 ttccggagcg aaccaagttt ttcttgcttg acagaatacg gcttccttcg acatcaatag
     2401 caagacctgg cacaagcact cctcctggat aaatatccat atcgcgctgc ttcccttcct
     2461 gatactataa tgagcagaag aattggcatg atgtgagata tagatagggc gggcttctac
     2521 ataatcaatg cagaagcccg ccgctccttt caagatctct tcaaatcaaa gcttcggtat
     2581 attcgctgcg agaataacac ctgctgctga ggcattaaga gctgctgaaa taatagacac
     2641 aattgcaaga agtgctggca aaaacgctcc ctcagtctcc ttcatctctt gctgcgataa
     2701 caccgccagt tgcatcggtt gatccgtctg cacgaacatc gtatcgatgt ccgtctgggc
     2761 gaaagctgcc actggcgggg cttgcacgtt ttcctgccgg gcctgattgg cgtaggcttg
     2821 gacttggacg gacaacaggc tagcagtgac cagtatgagc gtagcgcgtt taaatttttt
     2881 catataaact ttcataaact ttctagaaga tacaataata aatctcgaaa tcagctccga
     2941 gacacccatt accggtgttt gtaccaccgg caatccctgc acgggcggcc tgaccgcccg
     3001 tttttttcca aagatcaaag catgtataac cagttcaccc gtatctcggc accgtcgcgt
     3061 cccgatgtgt tgagcttgag cgtggtgctg agagtattgc ctttggcaag gccatagccg
     3121 acacccagca gcaggtcggt ggcagtacgg gcgataccct gggcctgtcc gtcgaagcgg
     3181 tcggcggcgt ggcgcgtcca ttgcacaccg gtggtcgtgg tcacgcgatc attgatggcg
     3241 aaagccagac cggggctgag caggagcaag ctgccgggct ggtaatgctg cgcaccgtct
     3301 gcgcgctcat gattgaagcg gtaggcgctg gtgagtgaaa acaccaccgg gtcgatagcc
     3361 ttgtagatcg ttaggcccag catggccgac ttaaagctcg agctgctgac gcggtgtttt
     3421 tcatgcaggg cgatttcggc aaagccaagg acggcggggg tgtcattgtc ctgcttgaac
     3481 tgatagttga ccccagccca ggcgtcggcg aagcgcgact cgctgctggt gcgggaaccg
     3541 tccagtccgc tgctgcgctg gctggatgcc aggccactga ggcgcgcata gatttcagct
     3601 ttggcggtga ggccatagcg caggcccagc gtggccacag tggtgtcgct gttgccaacg
     3661 ctctcaccga tccgcgtggg caatgtaatg aaagaggccg ggccggtttg aatggtgaca
     3721 ggatcggtgg cagagatgcc gtgccggtcg gcattggcgt aagacagaga agcttcgagt
     3781 ttgagcttgc ctttatcggt gatgagatct tcgaccgtca gcggcaggtc ggcccatgcc
     3841 agatgagaca gtgacaccag taggacaccc aagatgaaac gcatgcactc cctttcattt
     3901 tagtgtcccc ccgttttagt cttagtgata aataaaacca gaggggaaga tttatatcct
     3961 ctctttccat tgcttgtcaa tatttt
//
LOCUS       DASRDH010000024         2153 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1023988,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000024 DASRDH010000000
VERSION     DASRDH010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1023988"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="VFA14_00600"
     CDS             <1..269
                     /locus_tag="VFA14_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HZS79921.1"
                     /translation="EIANGFSELNDAEDQAARFRAQVTARDTGDDEAMYYDADYIRAL
                     EYGMPPAGGCGIGIDRLMMLITDAPNIRDVILFPHLRREDCPGL"
     gene            complement(282..1010)
                     /locus_tag="VFA14_00605"
     CDS             complement(282..1010)
                     /locus_tag="VFA14_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007882699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS79922.1"
                     /translation="MTIFTLFFHAAPGTRTRNRAIAGISLSILMHALLFFVLRSALQQ
                     VHVQADSSHEPLEVVFVQPRPTPPSPAVRQAGKSEPPPRIPSRLKPSPVAVPPEVDIS
                     TMLDAARERRRAAEDAAVRENAQARGLSANEIAHANMALQERKASGMFEIISKGPRVA
                     QYVFRSWTADAHHSQRQTITVDVGPDGDVEKAIVDSMITLIRKYYSGDFNWDSRRLGR
                     MVSLSARQEDTAGLRTFLLHEFFD"
     gene            1409..>2153
                     /locus_tag="VFA14_00610"
     CDS             1409..>2153
                     /locus_tag="VFA14_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007882714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HZS79923.1"
                     /translation="MLATALVSFPVRAQVATAAANQITSALPGVIPMPALPSSAVTNA
                     RAGPGDVLRVTVFGQRDLSAEVGVNDKGILTLPLIGGLDVTGLSTSEIAARVADALRA
                     GQYLRDPEVSVEAVQLRSQMVSVLGEVSRPGRYPIPGHLSVLELLATAGGLTAQADQS
                     VTLLRRKTGQNDTESDARIPILLGETHATERHPLDVELNNGDVVYVNRKKLFYIHGEV
                     HRPGSYPMEPDMNVMRAIALGGGMTQRASQ"
BASE COUNT          485 a          651 c          572 g          445 t
ORIGIN      
        1 gtgaaatcgc caatggtttc tcggagctga acgacgctga agatcaagcc gcgcgcttcc
       61 gggcgcaagt caccgccaga gacaccggcg atgatgaggc catgtactac gacgccgatt
      121 atatccgcgc gctcgaatat ggcatgccac cggccggtgg ttgcggcatc ggtatcgacc
      181 gcctgatgat gctgattacc gacgcaccca acatccgcga cgtgattctg ttccctcacc
      241 tgcgtcgcga agactgcccg gggctttgaa aacctgtccc ctcaatcaaa aaattcgtgc
      301 agcaggaatg tccgcaaacc tgccgtatcc tcctgacgcg ccgatagtga caccatgcgc
      361 ccgagccgtc gggaatccca gttgaagtcg ccggaataat atttccggat caacgtgatc
      421 atgctgtcga cgatggcctt ttccacatcg ccgtcgggac cgacatcaac cgtaatcgtc
      481 tgacgctggc tatggtgcgc atcggcggtc cagctgcgga agacatattg cgccaccctc
      541 ggccccttgc tgatgatctc aaacatgccg ctggccttgc gctcctgcaa agccatattg
      601 gcgtgcgcaa tctcattggc agacagtccg cgcgcctgcg cattctcgcg cacggcagca
      661 tcttcggcgg cacgacgccg ctcacgcgca gcatcgagca tggtcgagat atccacttcc
      721 ggcggcactg ctaccggaga cggcttcaga cgcgatggta tacgcggcgg cggctcagat
      781 tttccggctt gcctgacggc aggcgacggc ggtgttggtc gaggctggac gaaaaccacc
      841 tcaagcggtt catgcgagga atccgcctgt acatggactt gctgcaatgc ggaccgcaac
      901 acaaaaaaca gtagcgcatg catcaggatc gacagactga tgccagcgat agccctgtta
      961 cgggttcgcg ttccgggagc agcgtggaaa aatagcgtga aaatcgtcat accatacagc
     1021 agcaacggat aatatccggt ttttgcaagc catcatacct gatgcctgca gaaaatctga
     1081 caaaccgcac tgcccacagc gctcaccgac aatctgttgt ccacaaggaa aagttgccca
     1141 caaaaactgg gggaaagtct gtggataacg ccgaggattg ctcactatcc tgttgatttt
     1201 aaatcaattc aatgtcctga taaaaatcga tgcatgtcga gatatctttc tatgcatgtt
     1261 catctctacc tcaacaaaaa taaaactaaa tttatgtttt ttgcttaaaa tcaagaaaaa
     1321 tcgcacaata aagccgcaat tgttttgtca tcagcctcgg gagtgacact tgcgccgatt
     1381 tttcctttcc tcttttactg ctgccgccat gcttgccaca gccctagtga gtttccctgt
     1441 ccgcgcacag gtggcgaccg ctgcggccaa ccagatcact tccgctttgc cgggcgtgat
     1501 accaatgccg gcgctgcctt catcggcagt caccaatgcg cgcgcaggtc ccggtgatgt
     1561 cctgcgcgtg acggtgttcg gccagcgtga tctatcggcg gaagtcggcg tcaatgacaa
     1621 gggtatcctg acgctgccgc tgatcggcgg cctcgatgtc actggtctca gtaccagcga
     1681 aatcgccgcc agggttgctg acgccctgcg cgcaggccag tatctgcgcg atccggaagt
     1741 atcggtcgaa gccgtgcagc tacgcagcca gatggtgtcg gtactcggcg aagtcagccg
     1801 tccgggccgt tatccaatcc caggccatct gagcgtgctg gaattgctgg cgactgccgg
     1861 cggtctgacc gcccaggctg atcaaagcgt gacactgtta cgccgtaaaa ccggccagaa
     1921 cgacactgaa agcgatgccc gtattcccat tttgctggga gaaacccatg ccaccgaacg
     1981 ccacccgctc gatgtcgaac taaacaacgg cgacgtcgtc tacgtcaaca ggaaaaaact
     2041 cttttatatc cacggcgagg tccaccgccc cggttcctac cccatggaac cggacatgaa
     2101 cgtcatgcgc gccatcgcgt tgggcggcgg catgacgcaa cgcgcctctc agc
//
LOCUS       DASRDH010000025         2306 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_901372,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000025 DASRDH010000000
VERSION     DASRDH010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_901372"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(84..659)
                     /locus_tag="VFA14_00615"
     CDS             complement(84..659)
                     /locus_tag="VFA14_00615"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HZS79924.1"
                     /translation="MLLMIDNYDSFTYNIVQYFGELGEEVLTVRNDEITLDEIEKLDP
                     ERICLSPGPCSPKEAGICVDLLKRFSGRLPILGVCLGHQAIGAAFGGKVIRARQIMHG
                     KTSAIVHTGAGVFRNIPSPCNVIRYHSLAIERASLPGCLEVTAWTNDGEIMGVRHKEF
                     DLQGVQFHPESILSEHGYALLKNFLTGTAQS"
     gene            complement(661..2172)
                     /gene="trpE"
                     /locus_tag="VFA14_00620"
     CDS             complement(661..2172)
                     /gene="trpE"
                     /locus_tag="VFA14_00620"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HZS79925.1"
                     /translation="MTEPEFKSLASQGYNRIPLIAAAFVDLETPLTLYLKLAQTQNAG
                     KDTFLLESVVGGKRFGRYSLIGLPATTKLRSFGKQIEVLKHDQVIESLESNPLEFIAE
                     FQSRYQVAIRPGMPRFCGGLAGYFGYDAVRHIEKRLTDSTPKDDLGLPDIQLLVTEEL
                     AVIDNLSGKLYLIVYADPAQPNAFNQGCQRLQDLRAMLRRVADAPVTSGSTRTETIRE
                     FSREDYLKSVAKAKEYVMAGDLMQVQIGQRLKKSYVDPPLMLYRALRSLNPSPYMYFY
                     DFGDTQIVGASPEILVRAETIDKTSDGVAQKKIAIRPLAGTRPRGSTPEHDAQLATEL
                     LADPKEIAEHVMLIDLARNDIGRIATPGSVKVTEQMVIEKYSHVQHIVSNVEGLLKPG
                     LSNLDVLKATFPAGTLSGAPKVRAMEIIDELEPTKRGIYGGACGYLSFNGEMDLAIAI
                     RTGVIKHGMLYVQAAAGIVADSVPEMEWEETENKARAVLRAAEQVQDGLDGAI"
BASE COUNT          443 a          584 c          749 g          530 t
ORIGIN      
        1 tcgcgctgct caatacagcg cagcaaggct tcttgtggcg tgatggacat attggcttgg
       61 tatcgtttgc gaatcgctgg tacttacgac tgcgcagtgc cggtcaggaa atttttgagc
      121 aaggcgtagc cgtgttcgga cagaatcgat tccgggtgga attgcacacc ctgcaaatcg
      181 aattccttgt ggcgtacgcc catgatttca ccatcgttgg tccaggcggt gacttcgagg
      241 cagccgggca acgatgcgcg ctcgatggcc agcgaatgat agcggatcac gttgcaggga
      301 ctggggatgt tcctgaaaac accggcgccg gtgtggacga ttgccgatgt cttgccatgc
      361 atgatttgtc tggcgcgtat gaccttgcca ccgaaggctg cgccgatggc ttgatggccg
      421 aggcagacgc cgagaatcgg caaccggccc gagaaccgtt tgagcaggtc gacgcagatg
      481 ccggcttctt tcgggctgca cggaccgggc gacaggcaga tgcgttcggg atccagtttt
      541 tcgatttcgt cgagcgtgat ctcgtcgttg cggacggtca gcacttcttc accaagctcg
      601 ccgaaatact ggacgatgtt gtaggtgaag gaatcgtagt tatcgatcat gagcagcata
      661 ttagattgct ccatccaggc catcctgcac ttgttcggca gcgcgcagaa cggcgcgcgc
      721 cttgttttcg gtttcttccc actccatttc cggcactgaa tcggcgacga taccggcggc
      781 ggcctgcacg tacagcatgc catgcttgat gacgccggta cggatcgcaa tcgcgaggtc
      841 catttcgccg ttgaacgaca ggtagccgca ggcaccgccg tagatgccgc gtttggtcgg
      901 ctctagctcg tcaatgattt ccatcgcacg cactttcggg gcgcctgata gcgttccggc
      961 cgggaaggtg gctttcagca catcgagatt ggacaggccg ggtttgagca agccttcgac
     1021 attggagacg atgtgctgca catgggaata tttttcgatg accatctgct cggtgacctt
     1081 gacgctgcca ggggtggcga tgcggccgat gtcattacgc gccagatcga tcagcatgac
     1141 gtgctcggcg atttccttcg gatcggccag cagttcggtg gcaagttggg cgtcgtgttc
     1201 tggagtcgaa ccgcgaggcc gggtgccggc gagcggacgg atggcgattt tcttttgtgc
     1261 cacgccgtcg gaagtcttat cgatggtctc ggcgcggacc agaatttccg gtgaggcacc
     1321 gacgatctgc gtgtcgccaa agtcatagaa gtacatgtac ggcgatggat tcagtgaacg
     1381 tagcgcgcgg tacagcatca gtggtgggtc gacgtaggat ttcttcaggc gttggccgat
     1441 ctgtacctgc attaggtcgc cggccatgac gtattccttg gctttggcga cggacttcag
     1501 atagtcttcc ctggagaatt cacgaatggt ttcggtacgt gtcgaaccac tggtgacggg
     1561 ggcatcggcg acccggcgca gcatggcgcg caggtcttgc aagcgttggc agccctggtt
     1621 gaaggcgttg ggttgcgcag ggtctgcata gacgatgagg taaagtttgc cggagaggtt
     1681 gtcgatgacg gccagttctt cggtgaccag caactggatg tcgggcaggc cgagatcgtc
     1741 tttgggggtg ctgtcagtca ggcgtttctc gatgtggcgc acggcgtcgt aaccgaaata
     1801 gccggccagg ccgccacaga agcgcggcat gccagggcgg atcgcgactt ggtaacgtga
     1861 ctgaaattcg gcgatgaatt cgagcggatt gctttccagc gattcgatca cctggtcatg
     1921 cttcaggact tcgatctgtt tgccgaaact gcgcagcttg gtcgttgcag gcaggccgat
     1981 gagggagtaa cggccgaagc gtttgccgcc aactacggac tccagcagga aagtatcttt
     2041 gccagcgttc tgggtttgcg ccagcttgag gtagagtgtc agtggtgttt ccagatcgac
     2101 gaaggctgcg gcgatgagcg gaatgcggtt atagccttgt gaagccagcg atttgaattc
     2161 gggttcggtc atggtttctc tccaggactg ttattgtaag acgggttgca ggacgaacag
     2221 ggcgtcctgg caaacgcgtg ccgacgataa cgatgtcata caggggtaat gctgcaaaaa
     2281 cgttctggtc gaggggagcg gccagt
//
LOCUS       DASRDH010000026         2567 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1249973,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000026 DASRDH010000000
VERSION     DASRDH010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1249973"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..607)
                     /locus_tag="VFA14_00625"
     CDS             complement(<1..607)
                     /locus_tag="VFA14_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS79926.1"
                     /translation="MMSKEPLKCFSASALSIIPRFADSSRVRLAADAKFARSLSYRSI
                     ASALSLLFMGQLLPAKAQQTTLTAGQDVYTNGLNASKYDLLLGGNVTWRTAFSVNSAA
                     RSNIAIAGAGNTITAGTGNRLLSFSQNSNVSITDATITSQVFTSARDGLFKPDISNTT
                     VNLNLAGTTVRDIGPTDFNTNASADWGPIIVVRDGSNKVLNI"
     gene            complement(866..1321)
                     /locus_tag="VFA14_00630"
     CDS             complement(866..1321)
                     /locus_tag="VFA14_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006465401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HZS79927.1"
                     /translation="MKKLILVVVAASMLVAACAVTPNSASVYNTRQAQGAQTVRMGVV
                     DSVRNVTIDKGSSGVGTIAGGALGGIAAGSNIGKGSGALAAGIVGALVGGAAGNQIES
                     SIANRPGLEITVRLDNGELLAITQDADELFRVGERVRVLSSGRTTRVTH"
     gene            1471..1953
                     /locus_tag="VFA14_00635"
     CDS             1471..1953
                     /locus_tag="VFA14_00635"
                     /EC_number="3.2.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011615757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033958 - DNA-deoxyinosine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
                     /protein_id="HZS79928.1"
                     /translation="MSYKHSFAHVTDAHTHILILGSLPGEASLAHGQYYAHPRNSFWR
                     LMSEVIGENLHAMEYAQRLKTLLEHRVGLWDVIAAAQRKGSLDSNIADHTDNDLVSLL
                     DNLPELTTIGFNGGTAARLGLKILQQRASRYRIVQLPSSSPAHTMSYAQKLTQWKRIR
                     "
     gene            2015..>2567
                     /locus_tag="VFA14_00640"
     CDS             2015..>2567
                     /locus_tag="VFA14_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016833348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HZS79929.1"
                     /translation="MSNKRKSIQTQASAKASPGFAPVTLSEQDGVRYLHFGTEWVQGA
                     MRIRKPDWIELEYVQQMMSWMLFNPVPENIVQLGLGRGSLTKFCYRQFPAAHVTAVEL
                     NPSVIAVCHSMFGLPPEDSRLSLLNMDADDFIRNDRHSGAIDALQVDLYDAAARGPVL
                     DTPEFYTACRDSLGDNGIMTVNLF"
BASE COUNT          585 a          733 c          693 g          556 t
ORIGIN      
        1 cgatgttcaa caccttattt gagccgtcac gtaccacaat gatcggcccc caatccgccg
       61 aagcgttggt gttgaaatcg gtaggtccga tatcacgaac tgtcgtcccc gccagattca
      121 ggttgacggt ggtattgctg atatctggct tgaacaggcc gtctcttgcg ctagtgaaca
      181 cttgcgaggt aatcgtggcg tcggtgatgg aaacattgct gttctgagaa aaactcaaca
      241 gcctgttccc tgttcccgcg gtaatggtat tcccggctcc ggcgatggca atattgcttc
      301 tggcggctga gttgacgcta aaggccgtcc tccacgtcac gttgccgccg agcaggagat
      361 catacttact cgcgttgagt ccatttgtat aaacatcctg cccagcggtc aaggtggttt
      421 gttgcgcctt ggcaggcagc agctgcccca tgaacagcag ggaaagcgcg ctggcgatac
      481 tccggtatga caagctgcgt gcaaacttcg catcagcagc aagtcgcacc ctgctgctgt
      541 cggcaaaacg tggaatgatg cttaaagccg acgctgaaaa acatttcaga ggttctttac
      601 tcatcacctt gactttggaa gtagggtcag aaatttatac caataataag ggtttatatc
      661 atcgaaagca tgtgtttgtt agccatgcat caaagataaa cctggcgtat ttctcaatta
      721 cgtttatccg gatcagatac gcattccttg ctcagcacct ctgcatttcg ctctcgttgg
      781 gaagctggcg cgcggcgtga ttcgcgccgt caggaagcca tgatcgcacc agattccatg
      841 ttgccaaaaa gtcacaacgg ccaggttaat gcgtgacgcg cgtagtacgc cccgaggaca
      901 agacacgcac gcgttcgccg acgcggaata gttcgtcagc atcttgcgtg atggcgagca
      961 gctcaccgtt gtccagacgc acggtgattt ccaggcccgg ccggttggcg atgcttgact
     1021 cgatctgatt gccagccgcg ccgccgacca gggcgccaac gatgccagct gccagcgcgc
     1081 cgctaccttt accaatattg gaacctgcgg cgatgccgcc cagcgcgccg ccggcaatgg
     1141 tgccgacacc gctgctgccc ttgtcgatgg taacgttacg gaccgaatcg actacgccca
     1201 tgcgcaccgt ttgcgcaccc tgagcctgac gtgtgttgta gacactggcc gaattcggcg
     1261 tgacagcaca agctgccaca agcatgctgg cggcgacgac gacgagaatc aattttttca
     1321 tacagaactc caatggaaaa tgatgcccct tggggcgatg tattacacaa tgacaaaaag
     1381 aaaatgtcta cgtgcgccgg atagtgtagc ctcattcccc aggtcgcgtt acgatctcct
     1441 cacattccgg actgcccgct tccctgctcc atgtcataca aacacagctt cgctcacgtc
     1501 actgacgctc acacccacat cctgattctc ggcagcctgc ccggtgaagc ttcgctggcg
     1561 catggacagt attatgccca tccgcgcaac agcttctgga ggctgatgtc ggaagtcatc
     1621 ggcgaaaacc tgcatgcgat ggaatatgcg cagcgcttga agaccttgct cgaacatcgt
     1681 gtgggcttat gggatgtcat tgccgcagcg cagcgcaaag gcagcctcga cagcaatatt
     1741 gccgatcaca ctgacaatga cctggtcagc ctgctcgaca acctgccgga attgacgacg
     1801 ataggattca acggtgggac agcggcgcgt ttagggctga aaatactgca acagcgcgcc
     1861 agccggtatc gtatcgtcca gctaccgtca agcagccctg cgcatacgat gtcttatgcg
     1921 caaaagctga cgcaatggaa gcgtatccga tagcaggcgg caggtaaaac tgtccagcaa
     1981 ggtatcatga ggcaattcga gcgcgagcaa ggccatgtca aacaaaagaa aatccatcca
     2041 gacgcaagcc agtgccaaag ccagtcccgg attcgctccc gtcacgctgt cggagcaaga
     2101 cggagtgcgc tacctgcact tcggcacgga atgggtgcag ggcgcgatgc gtatccgcaa
     2161 accggattgg atcgaactcg aatatgtcca gcaaatgatg agctggatgt tgttcaatcc
     2221 tgtccccgag aatatcgtcc agctcggtct gggtagagga tcgctgacca aattctgtta
     2281 tcggcagttt cccgcggcac acgtgaccgc agtggaattg aatccatccg tgattgccgt
     2341 ctgccacagc atgttcgggc tgccgccgga agacagtcgc ctgtccctgc tcaacatgga
     2401 tgccgatgat ttcatccgca atgatcggca ttccggcgcc atcgatgccc tgcaggtcga
     2461 tctatacgat gcggctgcgc gtggcccggt gttggacacg ccggagttct acactgcctg
     2521 ccgcgacagt ctcggtgaca acggcatcat gacggtcaat ctgttcg
//
LOCUS       DASRDH010000027         4227 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_457360,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000027 DASRDH010000000
VERSION     DASRDH010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 4227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4227
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457360"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1180)
                     /locus_tag="VFA14_00645"
     CDS             complement(<1..1180)
                     /locus_tag="VFA14_00645"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZS79930.1"
                     /translation="MPIWNALRHTLEQEDKEGNMYTELHACRICGNTHLEPVLDLGEQ
                     TLTGVFPKTTGQAITRGPLRLVKCAGSDTCCGLLQLAHSYDLGEMYGDNYGYRSGLNA
                     SMVRHLNSKVQRLLGLVDMSGQPIILDIGSNDGTTLRAYGDRMGARVGIDPTADKFRQ
                     YYPPGVTIVPDFFSAQVFGQACPGGRARVVTCFSMFYDLERPMDFAREVASILDEKGI
                     WVCEQSYMPAMLKTNSYDTACHEHLEYYALKQIHWMMERVGLKIVDIEFNDVNGGSFS
                     VTAAQKGAAYPECAQLDELLARERAAGLDTLTPYQDFAMRVSANRQTLRAFVGQALGQ
                     GKRVACLGASTKGNVLLQYCGFTPEEIEAVGEVNPEKYGSFTPGTLLPIVPESELLAD
                     E"
     gene            complement(1134..2102)
                     /locus_tag="VFA14_00650"
     CDS             complement(1134..2102)
                     /locus_tag="VFA14_00650"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HZS79931.1"
                     /translation="MAASKTALITGISGQDGSYLAELLVGKGYRVIGTSRSDGTVPEI
                     GSIELRRLDLGDTDAVAELVAALRPDEIYNVAARASSAQLFDAPLETADINGLATVRF
                     LEAIRRYSPGTRFCQASSSEVFAGTRVSPQDESLDAAPVNSYGAAKAYGWHMVRMYRS
                     RYGLHACTAILFNHESPRRAGHHVTRRISTGVAMIAAGQADRLELGSLDSRRDWSFAG
                     DVVQAMWLMLQHATGDDYVVASGKTHSVRDFCRIAFECVGLDYEKYVTIDAAFGGRAE
                     TVELRGDPSRARRQLGWAPQMGFADMVRLIVDADLERLAAHARTRG"
     gene            complement(2137..3225)
                     /gene="gmd"
                     /locus_tag="VFA14_00655"
     CDS             complement(2137..3225)
                     /gene="gmd"
                     /locus_tag="VFA14_00655"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HZS79932.1"
                     /translation="MKNKVALITGITGQDGAYLAELLLSKNYVVHGIKRRASQFNTAR
                     VDHLYTDPHEENVRFFLHHGDLSDASNLTRIIQQTQPDEIYNLAAMSHVAVSFESPEY
                     TADVDGIGTLRLLEAVRLLGMTKKTRFYQASTSEMFGLVQEVPQKETTPFYPRSPYAI
                     AKLYAYWITVNYREAYGMYACNGILFNHESPLRGENFVTRKITRALTRIKLGLQDHLY
                     LGNLNALRDWGHARDYVEMQWLMLQQQQPEDFVIATGVQRSVRDFVNIAGQELGIFMR
                     WEGEGIDEKGYDQDGKCVVSVDPRYFRPTEVEALLGDSGKARARLGWAPKISFEELVR
                     EMVREDLKITQCDELSRRHGFSTNSYHE"
     gene            complement(3253..4038)
                     /locus_tag="VFA14_00660"
     CDS             complement(3253..4038)
                     /locus_tag="VFA14_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS79933.1"
                     /translation="MNTFNVCLGHLPFPRQYTHFIDLAIAPVVFEGAPRHTAIVPDEL
                     FGRHGHSLSEYAQLIWLRNHISGLAPDAQYVNIFHYRRFVSREPVDGPKSTNNAWVTM
                     VREDEVGAYAACFERQNHANCFNTPVHFPEGVLGQYASAHVLEDMLNFVKYLMEIEML
                     DSRQAVRFLRYEHLVPACSVGIYEVGIFRKLFAYLERAAEFLNTPYFIVRENYQRRVM
                     GFLLERLHSFLILEQLGHHGRFGHNMMISDSLEVTRTIRRVNG"
BASE COUNT          881 a         1315 c         1165 g          866 t
ORIGIN      
        1 gctcatccgc cagcagctcg gactcaggaa cgatgggcaa cagggtgccc ggggtaaagc
       61 tgccgtattt ttccgggttc acctcgccca cggcttcaat ctcttcaggc gtaaaaccgc
      121 agtactgcaa caacacattc cccttggtcg atgcccccag acaagccacc ctcttgcctt
      181 gcccaagcgc ctgcccgaca aaggcacgca gcgtctgccg gttcgcgctc acgcgcatgg
      241 caaaatcctg atagggggtc agggtatcca ggccggcagc gcgctcgcgc gccaacaatt
      301 cgtccaactg cgcgcactct ggataggcgg cacccttctg tgccgccgtc acggaaaagc
      361 tgccgccatt gacatcgttg aattcgatat cgacaatctt cagtccgact cgctccatca
      421 tccagtggat ttgtttgagg gcatagtact cgagatgctc atggcaggcc gtgtcatacg
      481 agttggtctt gagcatggcg ggcatatagc tttgctcgca tacccagatg cccttctcat
      541 cgaggatgct ggcgacctcg cgggcaaagt ccattggccg ctcgaggtca tagaacatcg
      601 aaaagcaagt gaccacgcgc gcgcggccac cggggcaagc ttgcccgaac acctgcgccg
      661 agaaaaaatc ggggacgatt gtcacgcctg gcggataata ctggcggaat ttgtccgcgg
      721 tcggatcgat gcccacgcgc gcgcccatcc tgtcgccgta ggcgcgcaag gtcgtaccgt
      781 cattgctgcc gatatccagg atgataggct ggccgctcat gtccaccagg ccgagaaggc
      841 gttgcacctt gctgttgaga tggcgcacca tgctggcgtt gagtccggaa cggtagccgt
      901 agttatcgcc gtacatctcc cccaaatcgt aggaatgcgc caactgcagc aagccgcagc
      961 aagtatcgct acctgcacac ttcaccaggc gcagcggacc gcgtgtgatg gcctggcccg
     1021 tggttttggg aaataccccg gtcaaggtct gctcccccag gtccagcacc ggttccagat
     1081 gcgtgttacc gcagatgcgg caggcatgca gttcagtgta catattgccc tccttatcct
     1141 cttgttcgag cgtgtgccgc aaggcgttcc agatcggcat cgacgatgag cctcaccatg
     1201 tcggcaaatc ccatctgagg cgcccaaccc aactgacgcc gcgcccgtga agggtcgcct
     1261 cgcagttcca ccgtttccgc acgaccaccg aaagcggcat cgatcgtcac atacttttcg
     1321 taatccagcc ccacgcactc aaacgcaatg cggcagaaat cccgcacgct gtgtgtcttg
     1381 ccagaggcga cgacatagtc atcgcctgtc gcatgctgca acatcagcca catggcttgc
     1441 acgacatcgc cggcaaatga ccagtcgcgc cggctgtcaa ggctacccag ttcgagacgg
     1501 tcggcctgac cggcagcgat catggccact cctgtgctga tacggcgtgt gacatgatgt
     1561 ccggcgcggc gcggactctc gtgattgaaa agaatcgccg tgcatgcatg cagcccgtag
     1621 cggctccgat acatccgcac catatgccat ccgtatgcct tggccgcacc atagctgttg
     1681 accggcgcag catccagcga ctcgtcttgc ggcgaaacgc gcgttcccgc gaacacctcg
     1741 ctgctggatg cctggcaaaa ccgcgtgccg ggactgtaac gccggatcgc ttccaagaag
     1801 cgcaccgtcg ccaggccatt gatgtcagcc gtttccaatg gcgcatcgaa caactgcgcg
     1861 ctggaagcgc gcgcggcaac attgtagatc tcgtcggggc gcagtgcggc caccagttcg
     1921 gcaacagcgt cggtatcacc caggtcgagc cggcgcaatt cgatgctgcc gatctccggc
     1981 acagtgccat cgctgcggct ggtgccgatg acgcgatagc ctttgccaac cagcagttcg
     2041 gcaaggtagc tgccatcctg gccggaaatg ccagtaatca atgccgtctt tgatgcagcc
     2101 actggctcct ctgtctggtt tatcgacgcc ggcacgtcac tcgtgatagc tgttggtaga
     2161 aaaaccgtgg cgtctgctca gttcgtcgca ctgcgtgatc ttcaggtctt cccgcaccat
     2221 ttcgcgcacc agttcctcga agctaatctt tggcgcccag cccagtctgg ccctggcctt
     2281 gccggagtcg cccaataatg cttccacctc ggtcgggcga aaatagcgtg gatcgacaga
     2341 gacgacacac ttgccgtcct ggtcgtatcc cttttcgtcg atgccctcac cttcccagcg
     2401 catgaagatg cccagctcct gtccggcgat attgacgaaa tcccgcacgc tgcgctggac
     2461 gccagtggcg ataacgaagt cctccggctg ctgctgttgc agcatgagcc attgcatctc
     2521 cacataatcc ctggcatggc cccagtcgcg caaagcgttc aggttgccca ggtaaaggtg
     2581 gtcctgcaag cccaatttga tgcgggtcag cgcgcgcgtg atcttgcgcg tgacaaaatt
     2641 ctcgccccgc aacggcgact catgattgaa cagtatgcca ttgcatgcat acatgccgta
     2701 tgcttcacga tagttgaccg tgatccaata ggcatacaac ttggcgatcg catacgggct
     2761 gcgcggatag aatggcgtcg tttccttctg cggcacttcc tgcaccaggc cgaacatctc
     2821 cgaagtagag gcctggtaaa agcgcgtctt tttcgtcatc cccagcaggc gcaccgcctc
     2881 cagcagccgc aatgtgccga tgccatcgac atcggcggtg tattcggggg actcgaaact
     2941 gaccgcaaca tggctcatgg ccgccaggtt atagatttca tcaggctggg tctgctggat
     3001 gatgcgcgtc aggttcgatg catcgctcaa gtcgccatgg tgcaggaaaa aacgcacatt
     3061 ttcttcgtgc ggatccgtat agagatgatc gacccgcgcc gtattgaatt gcgaggcccg
     3121 gcgcttgatg ccatgaacca catagttctt gctcagcaac agttccgcca ggtaggcgcc
     3181 atcttgcccc gtgataccgg tgatcaatgc aaccttattc ttcatctttc cttccttaga
     3241 gcgtgttagg cactaaccgt tgacgcgccg gatggtccgg gtcacttcca gtgagtcgga
     3301 aatcatcatg ttgtgaccga accggccatg gtgccccaac tgctccagga tcaaaaaact
     3361 atgcagccgc tcgagcagga aacccatcac ccggcgctgg tagttttcac gcacgatgaa
     3421 ataaggcgta ttgagaaatt cggccgcgcg ctccaggtag gcgaacagct tcctgaagat
     3481 tcccacttcg tagatgccta cgctacaagc cggcaccaga tgctcgtagc gcaggaagcg
     3541 gacggcctgg cgcgaatcga gcatttcgat ctccatcaaa tacttcacga aattgagcat
     3601 atcctcgagt acatgcgcac tggcgtactg gcccagcact ccctccggga aatggacagg
     3661 ggtattgaaa cagtttgcat gattctgacg ttcgaaacag gcagcgtagg cgccaacctc
     3721 atcttcccgc accatcgtga cccaggcatt gttcgtggat ttcgggccat ccaccggttc
     3781 gcgcgaaacg aaacggcgat agtggaaaat gttcacatac tgtgcatccg gcgccagtcc
     3841 gctgatatga ttgcgcagcc agatcagttg ggcgtattcc gagagcgaat gcccatgcct
     3901 gccgaacagc tcatcaggga cgatggccgt gtgcctgggc gctccttcga atacgaccgg
     3961 tgcgatggcc agatcgatga aatgcgtata ctgccgagga aagggtaaat gccccaggca
     4021 tacattgaag gtattcatct ccacaacctg ttcagaatcg tttcagtccg gctcacagat
     4081 tattccagtc cgacaaacgc agcaccggca attgcctgcg cggctgcacg gtgacggtgg
     4141 cagcaggcgg gaacggcacc agttgcgcct gctcgcgcac tggcttcccg ggggcgctgc
     4201 cctcttcggc cacggccagg ccgaagt
//
LOCUS       DASRDH010000028         6705 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_458047,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000028 DASRDH010000000
VERSION     DASRDH010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 6705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6705
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458047"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            102..854
                     /locus_tag="VFA14_00665"
     CDS             102..854
                     /locus_tag="VFA14_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010104363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZS79934.1"
                     /translation="MSQLMVENLHKCYGRNAVLKGVSVEAREGDVISIIGASGSGKST
                     FLRCINLLEKPTEGRIIVSGNQLKLRRNSSGDLDCIDTRQLQLVRAKLAMVFQHFNLW
                     SHMTALENIMEAPIHVLGVSRKQAMERARHYLAKVGVEGREDYYPSHLSGGQQQRIAI
                     ARALAMEPEVMLFDEPTSALDPELVAEVLRVMQDLAREGRTMLVVTHEMGFARNVSNQ
                     VIFLRQGVIEEQGDPREIFSNPQSEHLQKFLA"
     gene            886..1890
                     /locus_tag="VFA14_00670"
     CDS             886..1890
                     /locus_tag="VFA14_00670"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017879604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HZS79935.1"
                     /translation="MKRHAIDAAEAARALAEADFTDIPDRFGRAGFDVRLPARGILQI
                     TSPAQRGKRLSVLLSVGVHGDETAPIEMLAYLLTALARVPHALAVDLMVVVGNPDAIR
                     QGRRFVDVDMNRLFSSARKAPLQAAEAARADLIMSATAAFFSAPATAKWHLDLHTAIR
                     RSHYPKFAIIPEATGAAGKAAMLAWLGGAGIAAVILNPKPASTYSAYTAHEFGAVSCT
                     AELGQAGVLGRNDLHQLADMQAAIDALIRSGVTQPLHLHAPHVFQVVQEIIKRSDAFQ
                     MELDRATQNFTALAPEALIARDGASMYRVGPVTEYVVFPNPDVHIGQRAGLMVVLSEK
                     "
     gene            complement(2004..2351)
                     /locus_tag="VFA14_00675"
     CDS             complement(2004..2351)
                     /locus_tag="VFA14_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HZS79936.1"
                     /translation="MAKSNGRTGSSFDDFLKDEGIYEEVTAKALKRALTEQLRDIMVA
                     KHITKVEMANRMATSRTQVNRLLDPDNLRIQFDSLVRAASAMGKRVEIQLIDDPVTAV
                     VRTRPATRAKVAG"
     gene            complement(2344..2706)
                     /locus_tag="VFA14_00680"
     CDS             complement(2344..2706)
                     /locus_tag="VFA14_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HZS79937.1"
                     /translation="MSKLKRRPKLDARFYMTDMENEPVRVWLLKLPKEDRQQIGEEIA
                     YVQYKWPIGKPQVDHLSGPVWEVHTTLKTRIARALFAVEGNLMLLLHGFIKKNQSTPR
                     EDIALALTRYKDWKKHHG"
     gene            complement(2776..4686)
                     /locus_tag="VFA14_00685"
     CDS             complement(2776..4686)
                     /locus_tag="VFA14_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007880214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZS79938.1"
                     /translation="MAVISLSNAQLAFGHLALLDHAEFSLEHGERVALIGRNGSGKSS
                     LLQIIAGTSRLDDGLLVMQQGLKIAYVDQEPHFPAGMTSFDAVASGLGELPALQAEYE
                     ALTSQFGGNDDAALMERMHGIQSRLDAADAWNLDHRVNTTLDRLDLQGTTLIATLSGG
                     MQKRVALARGLVGAPDVLLLDEPTNHLDFSAIMWLEDLLRDYQGSVLFITHDRRFLDN
                     VATRIIELDRGRLLSFPGNFSTYQARKTELLANEEVENAKFDKFLAQEEVWIRKGVQA
                     RRTRDEGRVRRLEALRLERGARRERQGQVKLKVATGKRSGKIVAELENVSKSYGSQTI
                     VRDFTGTILRGDKVGLIGRNGVGKTTLLKLILGETSADTGTVRQGSKLQVAYFDQMRA
                     QLDEEASLADTIAPGSDWVEINGQRKHVMTYLADFLFAPERARSPVRTLSGGERNRLL
                     LARLFARPANVLVLDEPTNDLDIDTLELLEDLLEKYSGTVFLVSHDRTFLDNVVTQLI
                     AFEGDGLWREYIGGYSDWERVRADMQTTAKKSDSKPLETPPAPAALVVTATRPKKLSY
                     REQRELKELPELIAQLEEEQAVISTRLADPDLYRQGPDEARRLNARFAEIDTTLLNSL
                     ERWEAIEARQKM"
     gene            complement(4755..5954)
                     /gene="coaBC"
                     /locus_tag="VFA14_00690"
     CDS             complement(4755..5954)
                     /gene="coaBC"
                     /locus_tag="VFA14_00690"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HZS79939.1"
                     /translation="MDLAGKKILLGLTGGIACYKAAELARALGKAGASVQVVMTQAAT
                     RFITTITMQALTGNMVYTDPWDARIAGNMPHIDLTRGIDAIVIAPCSADFIFKLAHGA
                     CNDLLSTLCAARPHDVPLLIAPAMNVEMWQNPATQRNIALMQQDGIMLLGPDSGDQAC
                     GETGYGRMLEPERLLEEIIATFQPKLLASRRVLLTAGPTFEPVDPVRGMSNLSSGKMG
                     YAIARAAREAGAEVVLVSGPTALDTPYGVHRIDVQTAQQMHDAVFAQINGQDVFIAVA
                     AVADWRVANASARKLKKSAGDAAPQLILAQNPDILANVAALPERPYCVGFAAESEYLE
                     QYGAAKRIKKNIPLLVGNIGHQTFGKDENTLMLFDDKGRTTLPRASKKTLARQLVREI
                     AHRLTQA"
     gene            6165..6359
                     /locus_tag="VFA14_00695"
     CDS             6165..6359
                     /locus_tag="VFA14_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HZS79940.1"
                     /translation="MSQIPPERPDTPCVAVCSTTFDEVCRGCGRTVSEVARWVFMTKK
                     EKDAVWKRITAEGYPRRQPW"
     gene            complement(6347..>6705)
                     /locus_tag="VFA14_00700"
     CDS             complement(6347..>6705)
                     /locus_tag="VFA14_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020653251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HZS79941.1"
                     /translation="QLISMRGRCVIFHTALCLWDNRHPETPSIQIDNIQTFVTFRNLP
                     DTELDAYLRIEQPYDCAGSAKNEGLGIAILERIDSTDPTALTGLPLIALTGMLRNAGI
                     SFFGAAGQAPAIAYHG"
BASE COUNT         1323 a         1861 c         2008 g         1513 t
ORIGIN      
        1 cgcggtatgg cgctggcgaa ggtcgccggc aaggtggcgc agcgcactgg tgacttgctt
       61 ggtccgtagg cagcaatgaa acaggcatat aaggaaactc aatgtcccaa ttgatggtcg
      121 agaatttgca caaatgttac ggccggaatg ccgtactcaa aggcgtctct gtcgaagcgc
      181 gcgaggggga tgtgatcagt atcatcggcg cgtccggctc cggtaaaagc acttttttac
      241 gctgcatcaa cctgctggaa aagcccacgg aaggacgcat catcgtcagc ggcaatcaac
      301 ttaaactgcg ccgcaacagc agcggcgatc tggattgcat cgataccaga cagttgcagc
      361 tggtgcgcgc caaactggcc atggtatttc agcatttcaa tctgtggtcg cacatgactg
      421 cgctggaaaa tatcatggaa gcaccgatcc acgtgcttgg tgtatccaga aaacaagcga
      481 tggaacgcgc ccgccattat ctggccaagg tgggtgtcga ggggcgcgag gattattatc
      541 catcccatct ctccggtggt cagcagcagc gtatcgcgat tgctcgcgct ttggccatgg
      601 agccggaggt aatgctgttt gacgaaccga cttcagccct cgaccctgaa ctggtcgcgg
      661 aagtattacg ggtgatgcag gatttggcca gggaaggacg taccatgctg gttgtcaccc
      721 atgagatggg gttcgcgcgc aatgtctcga atcaagttat tttcctgcgt caaggcgtga
      781 tcgaggaaca gggcgatccc agggaaattt tcagcaatcc gcaaagcgag catctgcaga
      841 agttccttgc gtgagtgctg atgccgtata cccaacggag gacatgtgaa acgacatgct
      901 atagacgccg ccgaagcggc gcgcgcgctg gctgaagcag acttcacgga catcccggac
      961 cgctttgggc gcgccgggtt cgatgtgcgg ctgccggcca ggggcatact gcaaattaca
     1021 tcacctgcgc aacgtggcaa acgtctcagt gtcttgctgt cggtcggtgt gcatggcgat
     1081 gaaacggcgc cgatcgaaat gctggcgtat ctgctgactg ccctggcccg cgtgccgcac
     1141 gcgctggccg tggatttgat ggtcgtggtc ggcaatccgg atgcgatcag gcagggacgg
     1201 cggtttgtgg atgtcgacat gaaccgcctg ttttccagcg ccagaaaagc gccgctgcaa
     1261 gcagccgaag cggcgcgcgc tgatctcatc atgtctgcga cggcagcttt tttcagcgct
     1321 ccggcaacgg caaagtggca tctcgatttg catacggcca tacggcgctc gcattacccg
     1381 aagttcgcca tcattccaga agcgaccggc gctgccggga aagcggccat gctggcctgg
     1441 ctgggcggcg ccggcatcgc cgcggtgata ttgaacccga aaccggcatc tacctatagc
     1501 gcttataccg cgcatgaatt cggcgccgtc agttgcacag cggagttagg gcaggcgggc
     1561 gtgctggggc gaaatgatct gcatcagctg gccgacatgc aggcggcaat cgatgccctg
     1621 atacgctctg gagtgacgca gccattgcat ctccatgcgc cgcatgtgtt tcaggtggtg
     1681 caggaaatca tcaagcgcag tgatgcattc cagatggaat tggaccgggc gacgcaaaat
     1741 tttacggcgc tggcgccgga ggcgctcatc gcgcgggacg gggcaagcat gtaccgggtt
     1801 ggtccagtga cggaatacgt cgtgttcccg aatccggacg tgcatatcgg ccagcgcgcg
     1861 ggactcatgg tggtgctgtc ggaaaaatga tggtggcccg gccggtctgg caggctggcc
     1921 gggcggcttg aaagtgctat acatgtgtgc ctgggaacag ttcttgatct gaaagtcgtt
     1981 gtccgcccat attgcgaggt agttcatccc gccacttttg ctctcgtagc gggtcgcgtg
     2041 cgcactactg cggtaactgg atcatcaatc agctgaattt caacacgctt tcccattgca
     2101 gaagcagcgc ggacaagact gtcgaactgg atgcgcaagt tgtccggatc cagcaggcga
     2161 ttgacctgcg tgcgagacgt tgccatgcga tttgccatct cgaccttggt aatgtgcttg
     2221 gccaccatga tgtcgcgcaa ctgttcagtc aacgcgcgct ttagcgcttt cgcggtgact
     2281 tcctcgtaga tgccttcgtc cttcaaaaaa tcgtcgaaac ttgatccggt gcgtccgttc
     2341 gatttagcca tgatgttttt tccaatcctt gtaccgtgtt agcgccaaag cgatatcttc
     2401 tcgtggcgta ctttggttct tcttgataaa tccgtgcaac aaaagcatga ggttgccttc
     2461 gactgcaaac agcgcgcgtg caatccgcgt cttgagcgtc gtatgcactt cccaaacagg
     2521 tccgctcaga tggtcgacct gtggcttgcc aatgggccac ttatattgca cataggctat
     2581 ttcttcaccg atttgctgtc tgtcttcttt tggcaacttc aacaaccaaa cacgcaccgg
     2641 ctcattttcc atgtcggtca tgtaaaaacg ggcatctagc ttggggcgcc gcttcaattt
     2701 gctcatgttt ggttgaattg tacctcatgc ggtacatttt gcgagttatt taggaagtgc
     2761 aggcgtagta agaaattaca ttttctgacg tgcttcgatg gcctcccagc gctccaggct
     2821 gttcagcagg gtagtgtcaa tctcggcaaa gcgcgcgttc aggcgccgcg cttcatctgg
     2881 cccttgtcga tacagatccg gatcggccag tctggtggaa atcactgcct gctcttcttc
     2941 cagttgcgcg atcagctcag gcaattcttt cagctcgcgc tgctccctgt aactgagctt
     3001 tttgggccgg gttgccgtca cgaccaatgc tgctggcgct ggcggcgttt ccagcggttt
     3061 gctgtcgctc ttcttcgctg tcgtttgcat gtcggcacgc acacgctccc aatcgctgta
     3121 gccgccgata tattcacgcc ataggccatc gccttcgaaa gcgatgagct gcgtcacgac
     3181 gttgtcgagg aaagtacggt catggctgac caggaacacg gtgccgctat atttttccag
     3241 cagatcttcc agcagttcca gcgtatcgat atccagatcg ttggtcggtt cgtcgagtac
     3301 cagtacattg gccggcctgg cgaacagacg cgccagcagc aggcggttgc gttcgccgcc
     3361 cgacaaagtg cgtaccggcg aacgtgcgcg ttccggggcg aacaaaaagt cggcgagata
     3421 agtcatcacg tgcttacgct ggccgttgat ctccacccag tcgctgcctg gtgcgatcgt
     3481 gtcagccaga ctggcttctt catcgagctg ggcgcgcatc tggtcgaagt acgccacctg
     3541 tagcttactg ccctgccgca ccgtcccggt atccgcgctg gtttcgccga ggatcagttt
     3601 cagcagcgtc gtcttgccga cgccgttgcg gccgatcagg ccgaccttgt cgccgcgcag
     3661 gatggtgccg gtgaaatcgc gcacgatggt ttgcgatcca taagacttgc tgacattttc
     3721 cagttccgcc acgatcttgc cagaacgttt gccggtggca actttcagct tgacctggcc
     3781 ctggcgctcg cggcgtgcgc cgcgttcgag gcgcagggct tccaggcggc gcacacgacc
     3841 ttcgtcgcgg gtacggcgcg cctgcacgcc cttgcggatc cagacttctt cctgagccag
     3901 gaatttgtcg aacttggcgt tctcgacttc ttcattcgcc agcagttcgg tcttgcgcgc
     3961 ttgataggtg ctgaaattgc cggggaagga gagcagcctg ccgcggtcca gttcaatgat
     4021 acgggtggcg acgttgtcaa gaaagcgccg atcatgggtg atgaacagca cgctgccttg
     4081 atagtcgcgc aacaagtctt caagccacat gatggcgctg aaatccagat gattggttgg
     4141 ttcgtccagc aacagtacat cgggcgcgcc aaccaggccg cgcgccagcg ccacgcgttt
     4201 ttgcatgcca cccgacaggg tagcgattag ggtagtgcct tgcagatcga gccggtccag
     4261 tgtggtattg acccgatgat ccagattcca ggcgtcggcg gcatcgagcc tggattgaat
     4321 gccatgcatg cgctccatta gcgctgcgtc gtcattaccg ccgaactggc tggtcagcgc
     4381 ttcgtattcg gcttgcagcg ccggtaattc gcccagaccg gaagcgacgg cgtcgaagct
     4441 tgtcatcccg gccgggaaat gtggctcctg atcgacataa gcgatcttca acccctgctg
     4501 catgaccagc aggccatcat ccagtctgga ggtgccggcg atgatttgca gcaaggacga
     4561 cttgccgctg ccgttgcggc cgatcagtgc aacgcgctca ccgtgttcta gcgagaattc
     4621 cgcgtgatcg agaagcgcaa gatgtccaaa tgccagttgt gcattggaaa gagaaatgac
     4681 tgccataaat caacgatgcc gatggagacc attgcaggcc ggggaaagaa gcgaagcgcg
     4741 cgcattgtac cgcatcaggc ttgcgtcagc cgatgtgcaa tttcgcgcac cagttgccgt
     4801 gccagcgttt tcttgctggc gcgcggcagg gtggttctgc ctttgtcgtc gaacagcatc
     4861 agggtgtttt cgtctttgcc gaaggtctgg tggccgatat tgccgaccag caggggaata
     4921 ttcttcttga tgcgcttggc ggcgccatat tgttcgagat attcggattc agcagcaaaa
     4981 cccacgcaat aggggcgttc cggcaatgcg gcgacattgg cgagaatatc cggattttgc
     5041 gccaggatca gttgcggcgc tgcgtcgccg gcgctctttt tcagtttccg ggcgctggcg
     5101 ttggccacgc gccagtcggc taccgccgcc acggcgatga acacatcctg gccgttgatt
     5161 tgggcgaata cggcatcatg catctgctgc gccgtctgca catcgatgcg gtgtacgcca
     5221 taaggcgtat ccaatgccgt cggtccggac accagaacga cttctgcccc ggcttcccgt
     5281 gcggcgcgtg caatggcata tcccatcttg ccggacgaca gattgctcat accgcgcacg
     5341 ggatcgacag gctcgaaagt cgggccggct gtcagcagca cacgacgact ggccagcagc
     5401 ttcggctgaa acgtggcgat gatctcttcc agcaaccgtt ccggctccag catgcgtcca
     5461 tagccggttt cgccgcaggc ctggtcgcct gaatcggggc cgagcagcat gatgccgtcc
     5521 tgctgcatca gcgcgatatt gcgctgcgtt gccggatttt gccacatctc cacattcatg
     5581 gctggcgcga tcagcagagg tacgtcatgc gggcgcgccg cacacagtgt cgacagcagg
     5641 tcattgcaag cgccatgagc gagtttgaaa atgaaatcgg cggagcaggg cgcgatcacg
     5701 atggcatcga tgccacgtgt aaggtcgatg tgcggcatgt tgccggcaat gcgcgcatcc
     5761 cagggatcgg tgtagaccat gttgccggtg agtgcctgca tggtgatggt cgtgatgaac
     5821 cgtgtggccg cctgtgtcat gacgacctgc actgaagcgc cggctttacc gagcgcgcgc
     5881 gccagttcag cggccttgta gcaggcgatg ccgccggtca ggccgaggag aatttttttg
     5941 ccggcgagat ccattacctt attccttata aagcttgttt acgatcttct gagcaacaat
     6001 gccaggaagg ccatgaacga attgtaggct agtgttgatt caatgatcca gcatcggggc
     6061 atgagcgtga aggtatgcag tccggtacgc ttcgaggcgc tgtacccgcg cttcttggta
     6121 agatgtcggc tgcttgctgt tttttgatat ataaagatat attcatgtct cagattcccc
     6181 cggagcgtcc cgatacccct tgcgttgcag tctgttcaac caccttcgat gaggtctgtc
     6241 gcggctgcgg acgtaccgtt tccgaggtcg cgcgctgggt attcatgacc aaaaaagaaa
     6301 aagacgcggt ctggaaacgt attaccgccg aggggtatcc gcgccgtcag ccatggtaag
     6361 caattgccgg cgcctgaccg gcggcgccga aaaacgatat gccggcattg cgtagcatgc
     6421 cggtgagcgc aatcagcggc aggccggtga gggcggtggg gtcggtactg tcgattcttt
     6481 ccaggatggc gatacccagc ccttcgtttt ttgcgctgcc ggcgcaatcg tagggctgct
     6541 cgatgcgaag ataggcatcc agttcagtat ccggcaagtt gcggaatgtc acgaaggtct
     6601 ggatattgtc gatttgtatg cttggtgtct cggggtgacg gttgtcccac aggcacaatg
     6661 ctgtatgaaa aatgacgcag cgtccacgca tgctgatgag ttgct
//
LOCUS       DASRDH010000029         6260 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1022283,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000029 DASRDH010000000
VERSION     DASRDH010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 6260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6260
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1022283"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            34..1173
                     /locus_tag="VFA14_00705"
     CDS             34..1173
                     /locus_tag="VFA14_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014008184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HZS79942.1"
                     /translation="METLHQTPFSLSSATPATSSFARVSLLVYTLLIVYASCYPFAGW
                     RDMGISPFDYLWARLPYYWTSFDLWTNIAGYAPFGALMVFSLYPRLRSYRAVALSITA
                     GVALSAVMEAVQTYLPTRIPSNLDLMANSSGTMLGALLGAWSGRYFLRESRLLELRRR
                     WFSREAGRGLVVVGLWPLALIYPQNHLFGLGHLVPPLSSLLSDLPGKPLDLTTWWINH
                     AQLSAEQYWLADVIITACGVTGAVLTMLVLLRKQAPRNALMVLYLLSAICFKSLACAL
                     FFAPENAFVWYIPSTAGGMFVGALMLAGLSFAPPTAQRRTAALALIFSLSITNSVPTN
                     PYFISTLQTWVQDKFLNFNGAAQFLSISWPFLALWFLYHPVHHKS"
     gene            complement(1192..2637)
                     /locus_tag="VFA14_00710"
     CDS             complement(1192..2637)
                     /locus_tag="VFA14_00710"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HZS79943.1"
                     /translation="MIYLQPVILSGGSGTRLWPLSREQYPKQLLPLVGVDTMLQATAL
                     RIREGGCDYTLMEAPVVVCNEEYRFIIAEQLRAAGRPSRHIVLEPVGRNTAPALTLAA
                     LLACRDQADPILLAMPADHIICDEDAFQQAIKKGILHAAAGAIVTFGIVADRPETSYG
                     YIRRGKAVDGVHVIDCFVEKPDVATAQRYINSGDYFWNSGLFMMRASVWLRAISHFQP
                     AMAQACREAFDGGEQDHDFYRIDAAAFVACPADSIDYAVMEKLAEASVPDMEGRIIAL
                     AAGWSDIGSWDALWDVSEKDAAGNVARGNVMLAQASNSLVFAQERLVACVGIDNLVIV
                     ETPDAVMVADKSQMQEVRQIVAQLKGQKRSEALTHRKIYRPWGWYDSIDAGERFQVKH
                     IVVNPGSSLSLQMHHHRAEHWIIVKGTARITRGEDVFLLSENQSTYIPLGVMHRLENP
                     GKFPLEMIEVQSGSYLGEDDIVRFDDAYRRK"
     gene            complement(2809..3186)
                     /locus_tag="VFA14_00715"
     CDS             complement(2809..3186)
                     /locus_tag="VFA14_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HZS79944.1"
                     /translation="MLKKFLLALCAFGLSTGLAFAAVDVNKADQAALDGIKGIGPAMS
                     KRILDERQKGEFKDWTDLETRVKGIGDKSADKLSDAGLTVNGQQRNRSSAVGKGAAKP
                     AVKMKEVADAKMEKADKAAGVKD"
     gene            complement(3320..4249)
                     /gene="rfaE1"
                     /locus_tag="VFA14_00720"
     CDS             complement(3320..4249)
                     /gene="rfaE1"
                     /locus_tag="VFA14_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008114394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HZS79945.1"
                     /translation="MTVTTFFENKSSILIVGDVMLDRYWFGEVNRISPEAPVPIVRIE
                     RREERLGGAANVARNTATLGARTGLLGIIGQDEAGAVVEGLLTGMGIDSYLNRDPAIS
                     TIIKLRVIGRQQQLIRVDFEETPTGTVLRDKLTQFHALVGNYDVIVLSDYAKGSLVDV
                     ADMISVGRKLGKRMLVDPKGDDFSRYKGASILTPNCAELMCIVGPWKSEEELNTKAQN
                     LRQSLALEALLLTRSEEGMTLYLENEIVNFPAVAREVFDVSGAGDTVIATLAVMLGNG
                     VALPQAVAIANQAGGIVVGKLGTATVTREELAL"
     gene            complement(4246..5631)
                     /locus_tag="VFA14_00725"
     CDS             complement(4246..5631)
                     /locus_tag="VFA14_00725"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016833072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HZS79946.1"
                     /translation="MKITVIGTGYVGLVTGACLAELGNDVFCLDVDRHKIDILNSGGI
                     PIHEPGLEDVVTRNRVAGRLTFSTDVAASVAHGDIQFIAVGTPPDEDGSADLQYVLAA
                     AHNIGKHMSGFKVIVDKSTVPVGTAGRVHAAISATLQQRGLKDTDAAFSVVSNPEFLK
                     EGAAVEDFMRPDRIVIGCDDTPQGQQACAAMKKLYAPFNRHHERTCWMDVRSAEFTKY
                     AANAMLATRISFMNELANLADKVGADIEAVRYGIGSDPRIGHSFLYAGCGYGGSCFPK
                     DVRALEHIARDYGQELHILRAVEQVNKHQKHVLGKKIVARFGEKLQDRHFAIWGLAFK
                     PNTDDMREASSRVLLRELIERGATVAVYDPVAMKEAKRVLALDFAGSDSVLARVRFCS
                     GPMEALQDADALAIVTEWKAFRSPDFDQLKKRLRNPVIFDGRNLFEPQRMADIDIEYH
                     SIGRSFPTRTS"
     gene            complement(5664..>6260)
                     /locus_tag="VFA14_00730"
     CDS             complement(5664..>6260)
                     /locus_tag="VFA14_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HZS79947.1"
                     /translation="TNTDAAMSMLEKALAADRKSVRANILLGDVYLIKGDVEAALLVW
                     RRVEHQGVPYVVLVAQRLLDGYQTVGRMKEGLNLLRSYLAEAPSIDLLEVLFKAVLEL
                     EGVEAARQLVSDELRRTPTLLGLDKFLEACMIDAPAEIRPELSVVKTLVHSYTQKLAR
                     YQCTHCGFKARQFYWYCPGCNHWETYPPRRTEELNVMN"
BASE COUNT         1346 a         1913 c         1621 g         1380 t
ORIGIN      
        1 caagcgatgg agaaatcagc taccctcagc cgcatggaaa ccctgcacca aacacctttc
       61 tccctatcat ccgcaacacc ggcgacctca tcattcgccc gggtcagcct gctggtctac
      121 acgctgctga tcgtctatgc cagctgttac ccgtttgccg gctggcgcga catgggcata
      181 tcgcccttcg attacctgtg ggcgcgcctg ccgtattact ggaccagctt cgatctgtgg
      241 actaatatcg ccggttatgc gccgttcggc gcactgatgg tgttttccct ctacccccgc
      301 ctacgttcat accgggcggt ggcactcagc atcacggccg gcgtagcctt gtcggccgtg
      361 atggaagcgg tgcaaactta tctgccgaca cgtattccct ccaatctcga tctcatggcc
      421 aatagttccg gcacgatgct gggcgcattg ctgggcgcct ggagcgggcg ctatttcctg
      481 cgggaaagtc gcctactgga gctacggcgg cgctggttct cacgcgaggc cggccgtggc
      541 ctggtggtag tcgggctgtg gccgctggcg ctgatctatc cgcaaaacca tctgttcggc
      601 ctcggccatc tagtgccgcc gctgtcgagc ctgctctccg acctgcccgg caagcccctt
      661 gatctgacga cctggtggat caaccatgca caactgagcg ccgaacaata ctggcttgcc
      721 gacgtcatta tcaccgcctg cggcgtgacc ggcgcagtgc tgaccatgct ggtgctgctg
      781 cgcaaacagg cgccgcgcaa tgcgctgatg gtactctatc tgctgagcgc aatatgcttc
      841 aagtcactgg cctgcgcgct gttcttcgcg ccggaaaacg ccttcgtctg gtacatcccg
      901 agtacggcag gcggcatgtt cgttggcgcg ctgatgctgg ccggcctgtc gttcgcgccc
      961 cccacggcgc agcggcgcac tgcggcgctg gcactgattt tcagcttgtc aataaccaat
     1021 tcggtgccaa ccaatcctta tttcatctca acgttgcaaa cctgggtgca ggataaattt
     1081 ctcaacttca acggcgcagc gcaatttctt tccatatcat ggcccttcct ggcgctatgg
     1141 tttttgtatc acccggtaca tcacaagtcc tagtgaataa aagctcaggt tctacttgcg
     1201 cctgtaggca tcgtcaaaac gcacgatatc gtcttcgcca agatagcttc cagattgcac
     1261 ttcgatcatt tccaatggaa atttgccagg gttttccagc cggtgcatga cacccaacgg
     1321 aatataggtc gactggtttt ccgagagcag aaacacgtct tcgccacgcg taatgcgcgc
     1381 cgtgcctttg acgatgatcc aatgctcggc gcgatgatga tgcatctgca gactcaggga
     1441 tgaacccgga ttgaccacga tatgcttgac ctgaaagcgt tcaccagcgt cgatcgaatc
     1501 gtaccagccc cagggacgat atatcttgcg atgcgtcagc gcttcgctgc gcttctgccc
     1561 cttgagctgc gccacgatct gcctgacttc ctgcatctga ctcttgtcag cgaccatgac
     1621 ggcgtccggc gtttcaacga tgaccagatt gtcgatgccc acgcaggcga ccaggcgctc
     1681 ctgggcgaac accaggctgt tcgaagcctg cgccagcatg acgttgccgc gcgccacgtt
     1741 acctgcggca tccttctccg agacatccca caatgcatcc caagaaccga tgtcggacca
     1801 acccgccgcc agcgcaatga tacggccttc catgtccggc acagaggctt ccgccagctt
     1861 ttccatcacg gcatagtcga tggaatcggc tggacatgcc acaaaagccg ccgcgtcgat
     1921 gcgatagaaa tcgtgatcct gctcgccgcc atcgaaagcc tcacgacaag cctgcgccat
     1981 ggcaggctgg aagtggctga tggcgcgcag ccaaacactg gcgcgcatca tgaacaagcc
     2041 actgttccag aaataatcgc cgctgttgat ataacgctgc gccgttgcca catccggctt
     2101 ttcgacaaaa caatcgatga catgaacgcc atccacggcc ttgccgcgcc ggatgtagcc
     2161 atagctagtt tctggcctgt cggcgacaat gccgaaagtc actatggcgc cggccgccgc
     2221 atgcagaata ccttttttga ttgcttgctg aaaggcatcc tcatcacaaa tgatatggtc
     2281 ggccggcatc gccaacaaga tcggatcggc ctggtcccgg cacgccagca atgccgccag
     2341 cgtcaaggca ggtgcggtgt tgcgtcccac cggctcgagc acgatatgcc ggcttggtct
     2401 gcctgccgca cgcagctgct cggcgatgat gaagcggtat tcttcgttgc agacgacaac
     2461 cggcgcttcc atcagtgtat agtcgcagcc cccttcccgg atgcgcaagg cagtcgcctg
     2521 cagcatggta tcgacgccga ccagcggcaa cagttgtttg gggtactgtt cgcgagatag
     2581 cggccataaa cgcgtgcctg aaccgcccga aagaatgact ggctgcagat aaatcatcga
     2641 tctgtttcca cattattgaa caccagcctg ctacgaccgt tttttgctga tgcctcaaac
     2701 atcgacatcg ctcatcaggc caaccatcct atcatgcatg ccgcataaac agaaaacccc
     2761 gccaaagcag ggtttttcta acgtactgtg cgataaaaag cgccaggatc aatccttgac
     2821 accggcagcc ttatcggctt tctccatttt ggcatctgcg acttctttca tcttcacggc
     2881 aggtttcgcc gcgcccttgc cgaccgccga cgacctgttg cgctgctggc cattgacggt
     2941 cagaccggca tcagacagtt tatcggcgct tttgtcaccg atacctttga cgcgggtttc
     3001 aagatcggtc cagtccttga attcgccctt ttgacgttca tcgagaatcc gtttggacat
     3061 cgccggaccg atgcctttga tgccgtccag cgcagcctgg tcggctttgt tgacgtcaac
     3121 agctgcaaac gccaggccgg tgctcaggcc aaacgcgcaa agtgccagta agaatttttt
     3181 caacatgtta ttcctttctg agataagcat tatgttcatc aataacagat cagccagcaa
     3241 acaatcagcg agctaagtgt tttaacgatg ctgcggaata atagttgaca aaatagcaac
     3301 catatttaat tcaaataaat tagagcgcaa gctcttcccg cgtcaccgtc gccgtaccca
     3361 gcttgccgac gacgatgccg ccggcctgat tggcaatggc aaccgcctgc ggcaaggcaa
     3421 cgccattgcc aagcatcaca gccagtgtcg caatcactgt gtcaccggcg ccggagacat
     3481 cgaacacttc gcgcgccacc gccgggaaat tgacaatttc gttttccaga tacaaggtca
     3541 tgccttcttc ggaacgcgtc agcaacagcg cttccagcgc cagtgattga cgcagattct
     3601 gcgcctttgt gttcaattct tcttcactct tccaaggtcc gacgatacac atcaattcgg
     3661 cgcagttggg ggtcaggatc gacgcgcctt tgtagcgcga aaaatcatct ccttttggat
     3721 ccaccagcat gcgctttccc agtttgcggc cgacgctgat catgtcggcg acatcgacga
     3781 ggctgccttt ggcgtaatct gacagaacga tgacatcata gttgccgact agcgcatgaa
     3841 attgcgtcag tttgtcacgc aagaccgtgc cggtcggcgt ttcttcgaaa tccacgcgta
     3901 ttagttgctg ctggcgacca atcacacgca gcttgatgat cgtcgagatg gccgggtcac
     3961 ggttgagata gctatcgatc cccatcccgg tcagcaaacc ttcaacgacc gcgccggctt
     4021 cgtcttgccc gatgatgccc agcagaccag tccgcgcacc gagtgtagcc gtgttgcgcg
     4081 ctacgttggc agcgccaccc agacgttctt cgcgacgctc gatacgcacg attggcaccg
     4141 gcgcttctgg tgagatgcgg ttgacttcac cgaaccagta acgatccagc atgacgtcgc
     4201 cgacgatgag gatactcgac ttgttttcaa agaaagttgt gacggtcatg atgttctggt
     4261 cgggaaagaa cggccgatac tatggtattc gatgtcgata tccgccatgc gctgcggttc
     4321 gaataaatta cgtccatcga aaatgacagg gttcctgagc cgttttttga gctgatcgaa
     4381 gtctgggctt ctgaacgcct tccattcggt cacaatggcc agcgcatcgg catcctgcaa
     4441 tgcctccatc gggcctgagc agaaacgcac acgcgccagt acgctatcac tacctgcaaa
     4501 atccagcgcc agcacgcgtt ttgcttcctt catcgctacc ggatcgtaga ctgccacagt
     4561 cgcgccgcgc tcgatcaact cgcgcagcaa tacgcgcgaa gacgcttcac gcatatcgtc
     4621 ggtattgggc ttgaaggcca aaccccagat cgcgaaatgc ctgtcctgca atttttcgcc
     4681 gaaacgggcc acgatcttct tgccaagcac atgcttctga tgcttgttga cctgttccac
     4741 ggcacgcagg atatgcagtt cctgaccata atcgcgcgcg atgtgttcca gcgcccgcac
     4801 atccttggga aaacaggagc cgccataacc gcaaccggcg tacaggaaac tatgaccaat
     4861 gcgcggatcg gagccaatgc catagcgtac cgcctcgata tcggcgccga ctttatcagc
     4921 cagattcgcc aattcgttca tgaaagaaat gcgcgtggcc agcatggcgt tggcggcata
     4981 tttggtgaat tcggcagagc gcacatccat ccagcaggtg cgttcgtgat gacgattgaa
     5041 cggcgcgtac agttttttca tcgctgcaca ggcttgctgt ccttgtggtg tatcgtcgca
     5101 accgatgacg atgcgatctg gacgcatgaa atcttcgacc gccgcacctt ccttgagaaa
     5161 ctcgggattg gacaccacag agaatgcggc atcagtgtcc ttcaagccgc gctgctgtaa
     5221 cgtcgcgctg atggcagcat gcacacggcc ggcggtaccg accggcactg tcgacttgtc
     5281 aacgatgacc ttgaagccgc tcatgtgttt gccgatgttg tgcgcggcag cgagtacata
     5341 ttgcagatcg gccgaaccat cttcatcggg cggggtaccg accgcgatga actgaatgtc
     5401 accatgggcc acgctggcgg caacgtcggt cgaaaatgtc agccgaccag caacgcgatt
     5461 gcgcgtgact acgtcttcca gaccaggctc atggatagga ataccgccgc tgttgagaat
     5521 atcgatcttg tgtcgatcga catcgagaca aaacacgtcg ttacccaact ccgccagaca
     5581 agcgccagta accagaccga cataaccggt gccgataacg gtaattttca tagtagtcct
     5641 acagaggcga gtacaccggt actctagttc atcacattca attcttcagt tcgacgcggc
     5701 ggatacgttt cccaatggtt acacccaggg caataccagt agaactggcg cgccttgaag
     5761 ccacaatgcg tgcattgata gcgcgccagc ttctgcgtat agctatgcac cagcgtcttg
     5821 actaccgaca gctcaggacg aatttccgcc ggagcatcga tcatgcacgc ttccaggaat
     5881 ttgtccagac ccagcaacgt cggcgtgcgt cgcaattcgt cgctgaccag ctgcctggcg
     5941 gcttcgacgc cttccagctc cagtaccgcc ttgaacaaca cttccagcaa atcgatggac
     6001 ggcgcctcgg caagatagga acgcagcaag ttgaggccct ccttcatgcg gccgaccgtc
     6061 tggtagccat cgagtaaacg ttgcgccacc aataccacat acggcacgcc ttggtgctcg
     6121 acccgccgcc agaccagcaa tgcggcttcg acatcaccct tgatcaggta cacatcgcca
     6181 agcaggatat tggcgcgcac gctcttgcga tcggctgcca gcgccttttc cagcatgctc
     6241 atagcggcat ccgtattggt
//
LOCUS       DASRDH010000030         5141 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1607930,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000030 DASRDH010000000
VERSION     DASRDH010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 5141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5141
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1607930"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..710
                     /locus_tag="VFA14_00735"
     CDS             <1..710
                     /locus_tag="VFA14_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016346426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZS79948.1"
                     /translation="PRDLPVPGRELKGVHFAMEFLPLQNKVNAGDRLKGQIMATGKHV
                     VVIGGGDTGSDCVGTSNRHGAASVTQFELMPQPPEVENQPLVWPYWPTKLRTSSSHEE
                     GCDRDWAVATKRLEGKNGKVEKLIAVRVAWQGGKMREVPDSEFEMQADLVLLAMGFVS
                     PVQQVLDAFGVDRDQRGNARASTDGEGCYQTSVDKVFAAGDMRRGQSLVVWAIREGRQ
                     CARAVDEFLMGASLLPR"
     gene            729..2513
                     /locus_tag="VFA14_00740"
     CDS             729..2513
                     /locus_tag="VFA14_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007880729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HZS79949.1"
                     /translation="MRPPVRYSITSSDCAAHVFDLTLTVDAPAADGQVFSLPAWIPGS
                     YMMREFARHILQIRAEAAGRKVVLRKLDKHTWKAAPCAGPLTLSYQVYAWDLSVRTAH
                     LDQTHAFFNGASVFLRVEGQEQAQHVVDIRRPSGAAYARWRVATAMPTLRAQPYGFGT
                     YAASDYDELIDHPVELGNFALTGFTAHGVPHDIVITGKVPNLDLARLSDDLKKICEAQ
                     IAFFEPESRRAPMSRYVFMTLVLGDGYGGLEHRASAALICTRNDLPVKGKPETGDGYR
                     AYLGLCSHEYFHTWNVKRIKPAAFAPYDLRQESYTSLLWLFEGFTSYYDDLFLVRTGL
                     IDTPVYLNLLSRTINGVLRGSGRRKQSVAESSFDAWIKYYRQDENAPNAIVSYYAKGA
                     LVALALDLTIRSVTRKSLDDIMLALWQRYGRQFYNRDGGVGIGEDEALALFEEISGID
                     LKLFFDRYIHGTDDVPLQDLLASFGVTYADAGKETRPGMGIRVMRDGNEARIANAYEG
                     EPGQRAGLSAGDRLVALDGVRVPAAGMEGLLTRYRKNNTVELHVFRRDELMVFQVKLK
                     NDDAPQVRLSVQDKPAAVVKKRRAWLIR"
     gene            complement(2567..4213)
                     /locus_tag="VFA14_00745"
     CDS             complement(2567..4213)
                     /locus_tag="VFA14_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006402304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HZS79950.1"
                     /translation="MPLPKPPSQPATLLAPKDYPKAKRGKPVSKPHATSAKPAAPAVA
                     PVAKAVARATGVSGKSQAGKPHASSKPVASRTSRATSKPGASKLFVLDTNVLLHDPTS
                     LFGFKEHDIYLPMITLEELDDHKKGMSEVARNARQVSRSLDALVSDIAEDAIDLGIPL
                     SKLGNKDAKGSLFFQTRLPTNTKLPEGKADNQILEIVHALDQQYPDRPVVLVSKDINM
                     RLKARAIGLPAEDYLNDHVLEDSDLLYSGIQQLPDDFWRKHGKGMESWQENRRGSSYT
                     YYRITGPHVPSLLVNQFVFIEPGNSEPSFYGQVTQINGKTAVLQTLRDYSHTKNKVWG
                     ITSRNREQNFALNLLMNPECDFVTLLGQAGTGKTLLALAAGLTQVLETKTYNEIIITR
                     ATVPVGEDIGFLPGTEEEKMSPWMGAFDDNLEVLNKPDGDAGEWGRAANQDLIRSRIK
                     VKSLSFMRGRTFVNKFLIVDEAQNLTPKQMKTLVTRAGPGTKIICLGNIAQIDTPYLT
                     EGSSGLTYVVDRFKGWSHGGHVTLARGERSRLADHASTVL"
     gene            complement(4213..4701)
                     /locus_tag="VFA14_00750"
     CDS             complement(4213..4701)
                     /locus_tag="VFA14_00750"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HZS79951.1"
                     /translation="MSTNHTPSILNKTVPEFSAAMTSGKTFSLADYRDRNLVLYFYPK
                     DNTPGCATEGIQFRESYPEFQQHDTEIFGISRDSLKSHESFKARLSLPFELISDPDET
                     VCIMFDVMKMKNMYGKKVRGIERSTFVIDGSGTLVKEWRGVKLPLHVDEVLGFVKTMP
                     RF"
     gene            complement(4735..>5141)
                     /locus_tag="VFA14_00755"
     CDS             complement(4735..>5141)
                     /locus_tag="VFA14_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007882549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="xylanase"
                     /protein_id="HZS79952.1"
                     /translation="DGMLEDTAAGPHHLSVPGKALSCVQLPTTLPTLDELLGRTIDGV
                     VMDTSNVAAMLLRLTGQQVRDHVFTLHAELEGQKLAPVFEQLLLGWRAQGYDLVSMGD
                     YYRSVKDDILPILPVTWGKLPGRSGDLILQAA"
BASE COUNT          964 a         1371 c         1624 g         1182 t
ORIGIN      
        1 agccgcgcga cctgccggtg ccgggccgtg agttgaaggg cgtgcatttc gccatggaat
       61 tcctgccgct gcaaaacaag gtgaatgccg gtgacagact caagggccag atcatggcga
      121 ccggcaagca cgtggtcgtc atcggcggcg gcgataccgg ttctgactgc gtcgggacat
      181 ccaaccgtca cggcgctgct tcggtaaccc agttcgagct gatgccgcaa ccgccggaag
      241 tggaaaacca gccgctggtg tggccgtact ggccgaccaa gctgcgcacc tcctcatcgc
      301 atgaagaagg ttgcgaccgc gattgggcgg ttgccaccaa gcgtctggaa ggcaagaatg
      361 gcaaggtcga gaagctgatc gccgtgcgcg tcgcatggca gggcggcaag atgcgggaag
      421 tgccggattc cgaattcgaa atgcaggccg atctggtgct gctggcgatg ggcttcgtct
      481 cgccggtgca gcaggttctg gatgcctttg gcgtcgacag ggatcaacgc ggcaacgcca
      541 gggcaagcac cgatggcgaa ggctgttatc agacttcggt cgacaaggta tttgccgcag
      601 gcgacatgcg tcgcggccag tcgctagtgg tgtgggcgat tcgcgaaggc cgccagtgcg
      661 cgcgcgcagt ggacgagttc ttgatgggcg catcgctgct gccacgctga ttgactcatc
      721 attacgatat gcggccaccg gttcgttaca gtattacttc cagcgattgt gctgcccatg
      781 tgttcgatct taccctgacg gtcgatgcac cggctgctga cggtcaggta ttttcattgc
      841 cggcctggat tcccggcagt tacatgatgc gtgaattcgc gcgccatatc ctgcagatcc
      901 gcgctgaagc ggcggggcgc aaggtggtat tgcgcaagct cgacaagcac acctggaaag
      961 ctgcgccatg tgctggtccg ctgacgttga gttatcaggt ctatgcctgg gatctgtcag
     1021 tgcgcacggc gcatctggat cagacgcatg cctttttcaa cggcgccagc gtgtttttac
     1081 gtgtcgaagg gcaggagcag gcgcagcacg tcgtcgatat ccgccgtccg tccggcgctg
     1141 cctatgcacg ctggcgcgtg gcgacggcga tgccgacact gagagcacag ccttacggct
     1201 tcggtactta tgccgcaagc gactacgacg aattgatcga ccatccggtc gaactcggca
     1261 atttcgcgct gaccggcttt accgcgcatg gtgtaccgca tgacatcgtg attaccggca
     1321 aggtgcccaa tctcgatctg gcacggctgt cggacgatct caaaaaaatc tgcgaagcgc
     1381 agattgcttt cttcgaaccg gagagcaggc gcgcgccgat gtcgcgctac gtgttcatga
     1441 cgctggtgct gggcgacggt tacggcgggc tggagcaccg cgcatcagcg gcgctgatct
     1501 gcacgcgcaa cgatctgccg gtcaagggca agccggagac cggcgatggc tatcgggctt
     1561 atctcgggct gtgcagtcac gaatattttc acacctggaa cgtcaagcgc atcaaaccgg
     1621 cagcgtttgc accttatgat ttgcggcaag agagctacac ctcgctgttg tggctgtttg
     1681 aaggctttac cagttattac gacgacctgt tcctggtgcg taccggattg atcgatacgc
     1741 cggtttatct gaacctgctg tccaggacca tcaatggcgt tttgcgcggc agcggccgcc
     1801 gcaagcagag cgtggctgaa tcgagcttcg atgcctggat caagtactat cgccaggatg
     1861 agaatgcccc taatgcgatc gtcagttatt atgccaaagg cgcgctggtg gcgctggcgc
     1921 tggatctgac gatccgcagt gtcacgcgca agtcgctcga tgacatcatg ctggcgctat
     1981 ggcagcgtta tggccggcag ttctataatc gcgacggtgg cgtgggcata ggcgaagatg
     2041 aggccctggc cttgtttgaa gaaatcagcg gcatcgatct gaaacttttc ttcgaccgtt
     2101 atattcacgg caccgacgat gtgccgctgc aagacttgct ggcgtcattt ggcgttactt
     2161 atgccgatgc gggtaaggag accaggccag gcatggggat acgcgtgatg cgcgatggca
     2221 atgaggccag gatcgccaat gcttacgaag gtgagcctgg gcagcgcgcc ggtttgtctg
     2281 ccggcgatcg tctggtggcg ctcgatggtg tgcgtgttcc tgctgccggt atggaaggct
     2341 tgctgacgcg ctatcgcaag aacaacacgg tcgagctgca tgtgttccgt cgcgatgagc
     2401 tgatggtttt tcaggtaaaa ctgaagaacg atgatgcgcc gcaagtgcgc ttgtcggtac
     2461 aggacaagcc ggctgccgtc gtgaaaaagc ggcgagcctg gctgatacgt tgaaagaggt
     2521 agcgtaagaa cctattcaaa atcccgggac ggttcatttc cggatatcac aggacggtgc
     2581 tggcatgatc cgctagacgc gaacgttcgc cgcgcgccag agtgacgtgg ccaccgtgtg
     2641 accagccctt gaagcggtcg actacatagg tcaggccgct cgaaccttcg gtcaggtaag
     2701 gtgtatcgat ctgcgcgatg ttgccgagac agatgatttt ggtgccgggg ccggcgcggg
     2761 tcaccagggt tttcatttgc ttcggcgtca ggttttgcgc ttcgtcgacg atcaggaatt
     2821 tattgacgaa agtacggccg cgcatgaagc tcagcgattt gaccttgatg cgcgagcgga
     2881 tcagatcctg gtttgccgcg cgcccccatt cgccggcgtc gccgtcgggt ttgttcagca
     2941 cttccagatt gtcgtcgaag gcacccatcc agggactcat tttttcttct tcggtgccgg
     3001 gcaggaagcc gatgtcttcg cctaccggta cggtggcgcg ggtgatgatg atttcattgt
     3061 aggtcttggt ttccagcacc tgcgtcaggc cggcggccag tgccagcagc gtcttgccgg
     3121 taccggcctg gcccagcagg gtaacgaagt cgcattccgg attcatcagc aggttcagcg
     3181 caaaattttg ttcgcggtta cgcgatgtga tgccccagac cttgtttttg gtgtgactgt
     3241 agtcgcgcag ggtttgcagc acggcagtct tgccgttgat ttgcgtgact tgcccgtaaa
     3301 aggatggttc gctgtttcca ggttcgatga agacgaattg attgaccagc agcgagggca
     3361 cgtgcgggcc ggtgatacgg tagtaggtat agctgctgcc gcgccggttc tcttgccagg
     3421 attccatgcc tttgccatgc ttgcgccaga aatcatccgg caattgctgt atgcctgagt
     3481 acagcaggtc gctgtcttcc agcacatggt cgttgaggta atcttcggca ggcaaaccga
     3541 tggcgcgcgc cttgaggcgc atgttgatgt ctttggacac cagcaccacc gggcggtccg
     3601 gatattgctg atccagcgca tgcacgattt ccagaatctg attgtcggct ttgccttccg
     3661 gcagtttggt gttggtcggc agcctggtct ggaagaacag gctgcccttg gcgtccttgt
     3721 tgccgagttt ggagagcgga atgccaaggt cgatggcatc ttcggcgatg tcgctgacca
     3781 gtgcgtcgag cgagcgcgaa acctgacgcg cgttgcgggc gacttccgac atccccttct
     3841 tgtggtcgtc gagctcctcc agcgtgatca tcggtagata gatgtcgtgt tccttgaagc
     3901 cgaacagcga ggttggatca tgcagcagca cattggtatc gagtacgaac agcttggacg
     3961 cgccgggctt gctggtggcg cgactggtgc gtgatgcgac tggtttggag cttgcgtgcg
     4021 gtttcccggc ctggcttttg ccgctgaccc cggttgcccg cgctacagct ttggcgacag
     4081 gggcgacagc gggcgcggca ggcttggctg atgtggcatg gggcttgctg acaggtttgc
     4141 cgcgtttggc tttgggataa tctttgggcg ccaataaagt ggctggttga ctcggcggtt
     4201 tgggcagggg catcagaacc tcggcattgt tttcacgaaa cccagtactt catcgacatg
     4261 taatggcagc tttactccgc gccattcttt caccagtgtg cccgatccgt caatcacgaa
     4321 cgtgctgcgt tcgatgccgc gtactttctt gccgtacata ttcttcattt tcatgacatc
     4381 gaacatgata cagacagttt cgtcaggatc ggagatcagt tcgaagggta ggcttagtct
     4441 ggctttgaag ctttcgtgcg acttcaggct gtcgcggctg ataccgaaga tttcggtgtc
     4501 gtgctgctgg aattcaggat agctttcgcg aaactgtatg ccttcggtcg cgcagcctgg
     4561 cgtattgtct ttgggataga agtacagcac cagattcctg tcccggtaat ccgctagcga
     4621 aaaggttttg ccgctggtca tcgccgcgga aaattcaggt actgttttgt tcagtatgga
     4681 tggtgtgtgg ttagtcgaca tagcgtcctg aaagtggttg tatttgtcgg gaggtcaagc
     4741 cgcctgcagg atcaggtcgc cagaacggcc cggcagttta ccccatgtca cgggaagaat
     4801 cggcaggatg tcgtctttca cgctgcggta atagtcgccc atcgatacca gatcgtagcc
     4861 ttgcgcgcgc cagccaagta gcaattgttc aaagacgggg gcaagcttct gtccttccag
     4921 ttcggcgtgc agcgtgaata catgatcgcg cacctgctgc ccggtcagtc gcaacagcat
     4981 ggccgcgaca ttggatgtat ccatgacgac gccgtcgatg gtgcgtccga gcagctcgtc
     5041 gagcgtcggc agcgtggtcg gcaattgtac acaggatagg gctttgcctg gcaccgacag
     5101 gtggtgcggg ccggctgcgg tatcttccag catgccatct t
//
LOCUS       DASRDH010000031         2649 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1722363,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000031 DASRDH010000000
VERSION     DASRDH010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2649
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1722363"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="VFA14_00760"
     CDS             <1..364
                     /locus_tag="VFA14_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051262 - protein tetramerization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HZS79953.1"
                     /translation="KTLADGLFESTVTITVTSKIKDKVAFLVEGKQAGIFEIRNISAE
                     RLDPLLGIGCPNIIYPYLRANIADVVTRAGFLPIHLAEINFEVFYQQRLQALAKQQTT
                     NSTQPTNGTLVNGTDTVN"
     gene            428..880
                     /locus_tag="VFA14_00765"
     CDS             428..880
                     /locus_tag="VFA14_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HZS79954.1"
                     /translation="MQFPRLLITALLLGPAATTLADALDFKSVGATPAILYDAPSEKG
                     LRVFIAPRGMPVEMVLTYGDWSKVRDAAGTLSWVMSSALVTSRHVVVSVANARIYAAA
                     DDNSAAIFSADRNVLLELTGPTAISGWLKVKHQDGQAGFVKADEVWGD"
     gene            899..1900
                     /locus_tag="VFA14_00770"
     CDS             899..1900
                     /locus_tag="VFA14_00770"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HZS79955.1"
                     /translation="MNISILGAGAWGSALAIMLAARHDVILWGRNRQAMQMAAVQREN
                     MAYLRGCRFPESLEVTASFELAVEHLSRHPADGLLIVASPVSGLRALAEILRERAIPN
                     IVWLCKGLEEQTRLLPHQIVRDVLGDSVPAGALSGPSFAQEVAHGLPCALTIASSDKT
                     LRDHVVAAMHGGNIRIYSSDDLTGVEIGGAVKNILAIATGTADGLQLGLNTRAALITR
                     GLAEITRFGIALGGRMETFMGLSGVGDLILTCTGDLSRNRRVGLGLAQGKKLDAIVAE
                     LGHIAEGVHCARAVRSLARELHIDMPITNAVAGVLFDGMSVQEMVAQLLARDPRGEN"
     gene            complement(1906..>2649)
                     /locus_tag="VFA14_00775"
     CDS             complement(1906..>2649)
                     /locus_tag="VFA14_00775"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008444087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HZS79956.1"
                     /translation="QQQRLRDFPQAVWLDRLPAAFSGLVIGNEVLDAMPVQLLIRDGQ
                     GWLERGVALDDAGAFRYLDKPCGAALAASIPDAAALPAGYLTEVHPLAAGFMQSLAAM
                     LQNSKAAALLFDYGFPAREYYFPERTQGTLMCHYRHHAHPDPFYLPGLQDVTAHVDFT
                     AMARAAIDGGLQLLAYTSQAAFLLDAGIGELLLRTPVEETARYLPQANAVQKLISPAE
                     MGELFKMLALGNGIDWPQRLLAHDRTHCL"
BASE COUNT          546 a          857 c          762 g          484 t
ORIGIN      
        1 taaaacactg gccgatggcc ttttcgaatc caccgtcacc atcaccgtga cctccaaaat
       61 caaagacaaa gtcgctttcc tagtcgaggg caagcaagcc ggcatcttcg aaatacgcaa
      121 tatttctgcc gaacgactgg atccactgct gggcataggc tgcccgaaca tcatctatcc
      181 gtatctgcgc gccaacatcg ccgacgtggt cacccgtgct ggcttcctgc ctatccatct
      241 ggccgaaatc aatttcgaag tgttttatca acagcgcctg caagcgctgg ccaagcagca
      301 gaccaccaat agcacgcaac cgaccaatgg tacactggtc aacggcaccg acaccgtcaa
      361 ctgattccag atgccgcgca tgctgctacc ctgcccgtat gcgcgaacgc accagcggag
      421 caagtccatg caatttcccc gcctcctcat taccgccctc ctgctcggcc ctgccgcgac
      481 caccctggct gacgcgctcg acttcaagtc ggtcggcgcc acgccggcca tcctgtacga
      541 tgccccatct gaaaagggac tccgcgtctt catcgcgccg cgcggcatgc cggtcgagat
      601 ggtactgact tacggcgact ggagcaaggt acgcgacgcc gccggtacac tgtcgtgggt
      661 catgtccagt gcgctggtga ccagccgcca cgtcgtcgtc agcgtcgcca atgccagaat
      721 ttacgccgct gccgatgaca atagcgccgc catcttcagt gccgacagaa acgtgctgct
      781 ggaattgacc ggcccgactg ccatttccgg ctggctcaag gtcaaacacc aggatggcca
      841 ggccggcttc gtcaaggcgg atgaagtctg gggcgactaa gaacgtgttc acgttccaat
      901 gaatatttcc atactcggcg ccggcgcctg gggcagcgcg cttgccatca tgctggcagc
      961 gcgtcacgat gtcattctgt gggggcgtaa ccgccaggcc atgcaaatgg ccgccgtcca
     1021 gcgcgaaaac atggcctatc tgcgcggctg ccgtttcccg gaatcgctcg aagtcaccgc
     1081 cagtttcgaa ttggccgtcg aacacctcag ccggcatccg gccgacggcc tgctgattgt
     1141 ggcttcaccg gtctccggtc tgcgcgcgct ggctgaaatc ctgcgcgaac gcgcgattcc
     1201 caacatcgtc tggctttgca aaggtctgga agagcaaacc cgtctgctac cgcaccagat
     1261 cgtgcgcgac gttctgggcg acagcgtccc cgccggcgcc ttgtcaggcc catcgttcgc
     1321 gcaggaagtc gcgcatggcc tgccttgcgc actcaccatc gccagcagcg acaagacctt
     1381 gcgcgaccat gtggtcgccg ccatgcatgg cggcaatata cgcatctatt caagcgatga
     1441 tctgacgggt gtggaaatcg gcggcgcggt caagaacatc ctggcgatcg ccaccggtac
     1501 agccgacggc ttgcaattgg gcctcaatac ccgagcggcg ctgatcacgc gcggcctggc
     1561 ggaaatcacc cgcttcggca tcgcgctggg tggccgcatg gaaaccttca tgggactgtc
     1621 gggcgtaggc gatctgatcc tgacctgcac cggtgacctg tcgcgtaatc ggcgcgtcgg
     1681 cctcggcctg gcgcagggga aaaaactgga tgcgattgtc gccgaactcg gtcacattgc
     1741 cgaaggcgtg cattgcgcgc gggcagtgcg ctcactggcg cgtgagctgc atatcgacat
     1801 gccgatcacc aatgccgtgg cgggcgtgct gttcgatggc atgtccgtgc aggaaatggt
     1861 cgcgcaactg ctggcacgcg atccgcgcgg agaaaactga atctctcata gacagtgcgt
     1921 gcgatcatgc gccagcaggc gctgcggcca gtcgatgccg tttcctagcg ccagcatctt
     1981 gaacagctca cccatctcgg ccggagaaat cagtttttgc acggcattgg cttgcggcaa
     2041 gtagcgcgcc gtttcttcca ccggcgtgcg cagcaataat tccccgatgc cggcatcgag
     2101 caaaaacgcc gcctggctcg tataagcgag cagctgcaaa ccgccgtcga tggccgcgcg
     2161 cgccatcgcc gtgaaatcca catgcgccgt cacatcctgc aaacccggca ggtaaaacgg
     2221 atcgggatgc gcatgatgac gatagtgaca catcagcgtg ccttgagtgc gctcaggaaa
     2281 gtaatattcg cgggcaggaa aaccatagtc gaacagcaag gcggcagctt tactgttctg
     2341 caacattgcc gccagcgact gcataaaacc ggcagcaagc ggatgcacct cggtcagata
     2401 accggccggc aaggcagcag cgtccgggat agacgctgcc agcgccgcgc cgcatggctt
     2461 gtcgagataa cgaaatgcgc cggcatcgtc gagcgccacg ccgcgctcca gccagccctg
     2521 cccgtcgcgt atgagcaatt gcaccggcat ggcgtcgagc acttcgttgc cgatcaccag
     2581 accggaaaat gcagccggca aacgatcaag ccagacggct tgcggaaaat cgcgcagtcg
     2641 ctgttgttg
//
LOCUS       DASRDH010000032         2250 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1021658,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000032 DASRDH010000000
VERSION     DASRDH010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1021658"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..758)
                     /locus_tag="VFA14_00780"
     CDS             complement(<1..758)
                     /locus_tag="VFA14_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006400291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HZS79957.1"
                     /translation="MKNKASQEHIDVHVWEGKSDIFDRIARCLASFEIDVIRADHLDL
                     TQGQVSLTPSLAIISVTAIDRSGFNVTEWQKMHGMPVIWVAEAPRAYDARVYPPEYSH
                     ILMFDFTCSELRRLVWRLAGEMRSHPMSAQPRDPLIAESAVMQSLLKEAHVFADCDSN
                     VLITGETGVGKERIARLLHDHHRRYGQGPFVAVNCGAIPDGLFESHFFGHAKGAFTGA
                     VLAHKGYFEQADKGTLFLDEIGDLPLYQQVKLLRV"
     gene            complement(768..1136)
                     /locus_tag="VFA14_00785"
     CDS             complement(768..1136)
                     /locus_tag="VFA14_00785"
                     /inference="COORDINATES: protein motif:HMM:NF025475.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4282 domain-containing protein"
                     /protein_id="HZS79958.1"
                     /translation="MKDTGYLRRLRRHFNFEHLVTPLLIRLSYGVGLVVIVSAGLGAL
                     FVALTLPENSVPRAALTVGATLLALVLWRLLAELWMLAFNLHARLVEIRELLSQQRAE
                     SKARQETHRAAMILEAASDE"
     gene            complement(1164..>2250)
                     /locus_tag="VFA14_00790"
     CDS             complement(1164..>2250)
                     /locus_tag="VFA14_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS79959.1"
                     /translation="TTIGVANGQHFGSLALAAGVPGLAYSTIRAGIVEPPSIGIGGVG
                     ATAYTAQVRLYGKIQASVPLLLDVGLPLVIDVVNGQGTITDMCHIKDSVGNDTATIAV
                     SAPLLEACVGGITSGAAFSTTGSCDSQLQPATLVNVLGLLKLNTRVVIPALPNTGSVT
                     LSKGQTLTIGGNVLQIGTTVSNLFRAVLANILGQMLGQGQGGVTPSTLAAELLNASGN
                     VLAPALVDLRTFLTNLQGFANGLNDGSPLASIGNLVGGLINSVTNLLNNLLGNIVCRI
                     STVADPTKYNQCALTSQLSGGNGTAPNVLLVLLGLVQYVLAPLLDQVGAVLSSALGSL
                     LGLDVGQVDVTLIDLNCDSGTGVDLVY"
BASE COUNT          476 a          687 c          655 g          432 t
ORIGIN      
        1 acgcgcagca gcttgacctg ctgatagagc ggcagatcgc cgatctcgtc cagaaacagc
       61 gtacccttgt cagcctgttc gaaataaccc ttgtgcgcca gcacggcgcc ggtgaacgcc
      121 cctttggcat gaccgaagaa atgcgactcg aacaagccat ccggaatcgc gccgcaattg
      181 acggcgacga acggtccctg tccatagcgg cggtgatggt catgcagcaa gcgtgcaata
      241 cgttccttgc cgacgccggt ctcgccggtg atcaagacat tggaatcgca gtcggcaaag
      301 acatgcgcct ccttcagcag gctttgcatg acggcggatt cggcgatcag gggatcgcgc
      361 ggttgcgcgc tcatgggatg gctgcgcatt tctccagcca gacgccatac caggcggcgc
      421 agttccgaac aggtgaaatc gaacatcagg atatgcgaat attccggcgg gtatacccgt
      481 gcatcgtaag cgcgtggcgc ttccgcaacc cagatcaccg gcatgccatg cattttttgc
      541 cattcggtga cattgaagcc ggaacgatcg atcgcggtca ccgaaataat cgctagcgac
      601 ggcgtcagcg atacctgtcc ttgtgtcaga tccagatggt cggcacgaat gacatcgatc
      661 tcgaagctgg cgaggcaacg cgcgatacga tcgaaaatat cggattttcc ttcccagaca
      721 tgtacatcga tatgttcctg ggacgctttg tttttcatcg tcttccttca ctcatcggat
      781 gcagcctcca gtatcattgc agcgcgatgc gtttcctgtc tggctttgct ttcggctcgt
      841 tgctgcgaca acagctcacg gatttcgacc agccgcgcat gcagattgaa ggccagcatc
      901 cacagttcag cgagcaagcg ccacaacacc agcgccagca gggtggcgcc cactgtcagc
      961 gcagcgcgcg ggacgctgtt ttccggcagg gtgagcgcga cgaacagcgc ccccaggccg
     1021 gcgctgacga tcaccaccag ccccacccca tagctcagcc tgatcagcag cggcgtcacc
     1081 agatgttcga aattaaagtg acggcgcagc cggcgcaaat atcctgtgtc cttcatgatc
     1141 gggcatccct tgtcgttgac acgctaatac accaggtcga cgccagtgcc gctgtcgcag
     1201 ttgagatcaa tcaaggtcac atccacctgg ccgacatcga ggccgagcag actacccagc
     1261 gcgctcgaca gaacggcacc cacctgatcg agtagtggcg ccagcacata ctgcaccaaa
     1321 ccgagcagga ccagcagcac attcggggcc gtgccgttgc cgccgctcaa ttgactggtc
     1381 agcgcacatt ggttgtactt ggtggggtca gctacggtgc ttatccggca aacgatattg
     1441 cccaacagat tattcagcag gttggtgacg ctgttgatca gtccgccgac cagattgccg
     1501 atgctggcaa gcgggctgcc atcgttcaag ccattggcaa aaccttggag gttagtcagg
     1561 aaggttctca agtcaacgag ggccggagca agcacattgc cagacgcgtt gagcaactct
     1621 gctgccagcg tgctgggggt cacgccgccc tgcccctggc ccagcatctg gcccagtatg
     1681 ttcgccagca ccgcccggaa cagattgctg accgtggtgc cgatctgcag gacattgccg
     1741 ccgatggtaa gcgtttgtcc cttgctgagc gtcacgctgc cggtattggg caaggcggga
     1801 atcacaacgc gggtattcag tttgagcagt cccaggacat tgaccagagt cgccggctga
     1861 agctggctgt cacaggatcc tgtcgtcgaa aaggccgcgc cggaagtgat gccacccaca
     1921 caggcctcga gcagtggcgc cgaaacggcg atcgtggcgg tatcgttacc cacgctatcc
     1981 ttgatgtgac acatgtcggt gatcgtaccc tgaccattga cgacatcgat gaccagcggc
     2041 aggccgacat caagcagcag cgggacgctg gcctggatct tgccgtacag ccggacctgc
     2101 gccgtatagg ccgtcgcgcc cacgccgcct atgcctattg agggcggttc gacgatgccg
     2161 gcccgtatcg tggaataggc gagtcccgga acaccggcag cgagcgccag actgccaaaa
     2221 tgctggccgt tggcgacgcc gatggtggtc
//
LOCUS       DASRDH010000033         2294 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_456217,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000033 DASRDH010000000
VERSION     DASRDH010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456217"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            111..797
                     /locus_tag="VFA14_00795"
     CDS             111..797
                     /locus_tag="VFA14_00795"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HZS79960.1"
                     /translation="MTFKGKIMMGLSKQFVHFMENSQGAFWLKDTGSRCAYANRAVRE
                     LLRLPHGFEVEGLRVGEIPAPIAEFEKEFEESDRLAEVTQETKWSLEIHPFGKDQVLS
                     AYFCEKSPLLNGNNEVIGTILHSYPALNLRFNRDKIDAGVFIFGVPDDTLSKKEWDFV
                     YFVIHGYSIKEMARLSGVSPGTIASRQNSVFNKLGIDTLAQLKQMAVAKGWDNYIPTD
                     LLHNRVIKLW"
     gene            complement(863..1228)
                     /gene="secG"
                     /locus_tag="VFA14_00800"
     CDS             complement(863..1228)
                     /gene="secG"
                     /locus_tag="VFA14_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007881945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HZS79961.1"
                     /translation="MDTLFMVIVVVQVLAALLIIGLVLLQHGKGADMGAAFGSGASGS
                     LFGATGSSNFLSKSTGIAATVFFIATLMLAFVGNRHVSQSGGVMDSLPAPTAPVPTAS
                     AAPGVPEAASADRSDQIPE"
     gene            complement(1256..2008)
                     /gene="tpiA"
                     /locus_tag="VFA14_00805"
     CDS             complement(1256..2008)
                     /gene="tpiA"
                     /locus_tag="VFA14_00805"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008119248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HZS79962.1"
                     /translation="MRRQLVAGNWKMNGGLAANAALVADIRTGLMGAGARVDVAVCVP
                     APYLAQVQVAVAGSPLALGAQDVSAHAGGAHTGEVSAGMLAEFSCRYVIVGHSERRAC
                     HGETDVVVVAKTAAALQSGLTPIVCVGETLEEREAGLTDVVVGRQIDALLDAVDWGKI
                     VVAYEPVWAIGTGRTATPEMAQDTHAKLRGRLAARDGAAAAAVRILYGGSMKPDNAQA
                     LLVMPDIDGGLIGGAALKAADFLAIVQAAQGD"
     gene            complement(2064..>2294)
                     /locus_tag="VFA14_00810"
     CDS             complement(2064..>2294)
                     /locus_tag="VFA14_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HZS79963.1"
                     /translation="SQVLRRRLTVTGSTLRSRPAAFKAHIASRLKERVWPLLASGRIK
                     PVIYRTFPLAQAAQAHALMESGAHVGKIMLLV"
BASE COUNT          542 a          702 c          603 g          447 t
ORIGIN      
        1 gcgaagggct gggacaacta tatcccgacc gatctgctgc ataacaggat gatcaaactg
       61 tggtgacaaa ccctgcccgc ttgcattact gtgtgacgtc ttaatcagcc ttgaccttca
      121 aaggaaaaat tatgatggga ttatcgaagc agtttgttca ttttatggag aattcccaag
      181 gagcattctg gctcaaagat acgggctctc gttgcgcgta tgcaaaccgg gctgttcggg
      241 aactgttgcg gttgccacac ggatttgaag tcgaaggctt acgggttggt gaaatacctg
      301 ctccaatagc tgaattcgag aaagagtttg aagagtctga tcgcctggct gaggtcactc
      361 aggaaacgaa gtggtctctg gaaattcacc cattcgggaa agaccaggtg ttgtctgcct
      421 atttttgcga aaaatctcca ttactcaacg gaaacaacga agtaattggc acgattctcc
      481 actcttatcc ggcattgaac ctgcggttca acagggacaa gatagatgcg ggagttttta
      541 tctttggcgt accggacgat accctgtcca aaaaagagtg ggatttcgtc tatttcgtaa
      601 tacacggtta ttcgatcaag gaaatggcgc gtttatcagg agtctcaccg ggtacgattg
      661 ccagccggca gaattccgtc ttcaacaaac taggcatcga cacgcttgcc cagctcaaac
      721 agatggcggt ggcgaagggc tgggacaact atatcccgac cgatctgctg cataacaggg
      781 taatcaaact gtggtgatag ctcttccagc atcacctggg tgcaggatat ttgagcgcgg
      841 gtcattcagg ctgacctgaa gactattccg gaatctgatc cgatcgatcc gcggacgctg
      901 cctccggcac gcccggagct gccgacgcag tcggaaccgg agcagtcggc gccgggaggc
      961 tatccatcac gccgccgctt tgcgacacat gacgattgcc gacgaaagcc agcatcagcg
     1021 tggcgatgaa aaagaccgtc gcagcaatgc ccgtagactt cgacaaaaaa ttggaggatc
     1081 ccgtggcgcc gaacaggctg cccgaggcgc ccgaaccaaa cgccgccccc atatccgccc
     1141 ccttgccatg ctgcagcagc accagaccga tgatgagcag cgctgccagc acctgtacca
     1201 ccacaatcac cataaacaga gtatccatat caccatcttc aatgtgaaac aaccatcaat
     1261 cgccctgcgc ggcctgtacg atcgccagga aatccgccgc cttcagggcc gcgccaccga
     1321 tcagaccacc gtcgatgtcc ggcatgacca gcaatgcttg tgcattatcg ggcttcatgc
     1381 tgccgccata caggattctg actgcggcgg cagccgcgcc atccctggcc gccagcctgc
     1441 cgcgcagctt cgcatgcgta tcctgagcca tttccggcgt ggcggtcctg ccggtaccaa
     1501 tcgcccagac tggctcatag gccaccacga tctttcccca gtcgacggca tccagtaacg
     1561 catcgatctg gcggccgacg accacatcgg tcaaccccgc ttcacgctct tccagcgtct
     1621 cgcccacaca gacgatcggc gtcaatccgg attgcagcgc ggcggcagtt ttggccacca
     1681 ccaccacatc ggtttcacca tgacaggcgc gccgttcgga atgaccgacg atgacgtagc
     1741 ggcaggaaaa ttcagccagc atgccagccg acacttcgcc ggtatgggcg ccaccggcat
     1801 gcgcagaaac atcctgcgcg cccagcgcca gcggagaacc tgcaacggcc acctgcactt
     1861 gcgccagata aggcgcggga acacagaccg caacatcgac ccgtgcgccc gcgcccatga
     1921 gccccgtcct gatatcggcg accagcgctg cattggcggc aagcccgcca ttcatcttcc
     1981 agtttccggc aactaattga cgacgcatga gaggaaagca ctgtaaaaaa tttttctatt
     2041 ctatcgtgcc gcagccaacc cggtcaaacc aatagcatga tcttgcccac gtgcgcgccg
     2101 gactccatca aggcatgcgc ctgcgcagcc tgcgccagcg ggaatgtccg ataaatcacc
     2161 ggtttgatcc tgccactggc aagcaggggc cagacacgtt ctttcaaccg gctggcgata
     2221 tgggccttga acgctgccgg acgcgaacgc aaggtcgaac cggtcaccgt cagacgccgc
     2281 cgcagcacct gact
//
LOCUS       DASRDH010000034         4089 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1247286,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000034 DASRDH010000000
VERSION     DASRDH010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 4089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4089
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1247286"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..75)
                     /locus_tag="VFA14_00815"
     CDS             complement(<1..75)
                     /locus_tag="VFA14_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HZS79964.1"
                     /translation="MDKKQSRLRRAAQTRAKIAELKVNR"
     gene            complement(86..619)
                     /gene="rplF"
                     /locus_tag="VFA14_00820"
     CDS             complement(86..619)
                     /gene="rplF"
                     /locus_tag="VFA14_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012734367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HZS79965.1"
                     /translation="MSRVAKMPIALPNGAEATITAAQITVKGPLGSLSQSLTGLVKIA
                     NDNGTLNFEAADGSREANAMSGTLRALVNNMVNGVTKGFEKKLSLVGVGFRAQAQGDK
                     LNLSLGFSHPIIHQMPQGIKCETPTQTEILIKGIDRQVVGQVAAEIRAYRQPEPYKGK
                     GVRYLDEVVVLKETKKK"
     gene            complement(632..1027)
                     /gene="rpsH"
                     /locus_tag="VFA14_00825"
     CDS             complement(632..1027)
                     /gene="rpsH"
                     /locus_tag="VFA14_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HZS79966.1"
                     /translation="MSMSDPIADMLTRIRNAQGVQKTTVVIPSSRVKIAIATVLKDEG
                     YIEDFAVTEAGGKAELKIALKYYAGRPVIERLERVSRPGLRIYKGKDEIPSVMNGLGV
                     AIVSTPRGVMTDRKARATGVGGEVICYVA"
     gene            complement(1042..1347)
                     /gene="rpsN"
                     /locus_tag="VFA14_00830"
     CDS             complement(1042..1347)
                     /gene="rpsN"
                     /locus_tag="VFA14_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HZS79967.1"
                     /translation="MAKLALINREQKRADLVKKYAGKRAELRAVIDDQSRSEGERYEA
                     RLKLQALPRNSAPTRQRNRCALTGRPRGTFRKFGLGRIKVREIAMRGEIPGLTKASW"
     gene            complement(1355..1894)
                     /gene="rplE"
                     /locus_tag="VFA14_00835"
     CDS             complement(1355..1894)
                     /gene="rplE"
                     /locus_tag="VFA14_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HZS79968.1"
                     /translation="MARLQEFYKEKVIADLTAKYAYKSVMEVPRILKITLNMGLSEAV
                     ADKKIIEHAVGDLTKIAGQKPVVTKARKAIAGFKIREGYPIGCMVTLRGAQMYEFLDR
                     FVTVALPRVRDFRGVSGRAFDGRGNYNIGVKEQIIFPEIEYDKIDALRGLNVSITTTA
                     KTDDEAKALLAAFKFPFRN"
     gene            complement(1901..2215)
                     /gene="rplX"
                     /locus_tag="VFA14_00840"
     CDS             complement(1901..2215)
                     /gene="rplX"
                     /locus_tag="VFA14_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HZS79969.1"
                     /translation="MGKIRKNDEVIVLTGKDKGKRGVVTQRVDADYVVVEGVNVAKKA
                     VRPNPMTGATGGIVNKLMPIHVSNVALFNAASGKADRVAYKSVDGKKVRVYKSSGEVV
                     KV"
     gene            complement(2225..2593)
                     /gene="rplN"
                     /locus_tag="VFA14_00845"
     CDS             complement(2225..2593)
                     /gene="rplN"
                     /locus_tag="VFA14_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HZS79970.1"
                     /translation="MIQTESRLEVADNTGAREVLCIKVLGGSRRRYAGIGDVIKVTVK
                     SAAPRGRVKKGEVYNAVVVRTAKGVRRQDGSLVKFDGNAAVLLNAKLEPIGTRIFGPV
                     TRELRTERFMKIVSLAPEVL"
     gene            complement(2790..3062)
                     /gene="rpsQ"
                     /locus_tag="VFA14_00850"
     CDS             complement(2790..3062)
                     /gene="rpsQ"
                     /locus_tag="VFA14_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HZS79971.1"
                     /translation="MNDQVKKALKRTLIGKVVSDKMDKTVTVLIERHVKHPLYGKIIV
                     RTNKYHAHDEMNQAKTGDTVEIQEGRPISRTKSWSVTRVVQVAQLV"
     gene            complement(3059..3250)
                     /gene="rpmC"
                     /locus_tag="VFA14_00855"
     CDS             complement(3059..3250)
                     /gene="rpmC"
                     /locus_tag="VFA14_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HZS79972.1"
                     /translation="MKASELRGKDEADLFKELNELLKAQFSLRMQVATQQLNNTAQLK
                     KIRRDIARVKTVINSKGQQ"
     gene            complement(3263..3682)
                     /gene="rplP"
                     /locus_tag="VFA14_00860"
     CDS             complement(3263..3682)
                     /gene="rplP"
                     /locus_tag="VFA14_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006464927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HZS79973.1"
                     /translation="MLQPARRKYRKEQKGRNTGISHSRGTSVSFGEFGLKAVGRGRIT
                     ARQIEAARRAMTRHIKRGGRIWIRIFPDKPISQKPAEVRMGNGKGNPEYYVAEIQPGK
                     MLYEMDGVDETLAREAFRLAAAKLPLSTTFVVRQVGQ"
     gene            complement(3685..>4089)
                     /gene="rpsC"
                     /locus_tag="VFA14_00865"
     CDS             complement(3685..>4089)
                     /gene="rpsC"
                     /locus_tag="VFA14_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HZS79974.1"
                     /translation="NAMRLGAQGIKIMSSGRLNGIEIARQEWYREGRVPLHTLRADID
                     YGFSEAQTTYGIIGIKVWVYKGDRLANGEAPVLVGESEDDRKRRGPRRDDGKPGARPR
                     SKTGGAPAAGVGTKRAPRQSADVTVSAEKAGE"
BASE COUNT          947 a         1180 c          927 g         1035 t
ORIGIN      
        1 acgatttacc tttaattctg cgatttttgc acgggtttga gccgcacggc gcagacgtga
       61 ttgtttttta tccatcgtca accccttact tcttcttggt ttctttgagc acaaccactt
      121 cgtccaaata acgaacacct ttgcctttat aaggctccgg ctgacgatat gcacggattt
      181 cagcggcgac ctgaccaact acctgacggt cgataccctt gatcaagatt tcggtttgtg
      241 tcggggtctc acatttgatg ccctgtggca tctggtgaat gatcgggtgt gaaaaaccca
      301 gtgacaaatt caacttgtca ccttgcgcct gcgcacgaaa acccacgcca accaggctca
      361 atttcttttc gaatcccttg gtgacgccgt tgaccatatt attgaccaac gcgcgcaaag
      421 taccagacat cgcattcgcc tcacggctgc catccgcagc ttcgaaattc agcgtgccat
      481 tatcgtttgc aatctttacc aggccagtca gtgactgaga aagcgaaccc agcgggcctt
      541 ttactgtaat ttgcgctgca gtaatggtcg cttcggcgcc attcggcagt gcaatcggca
      601 ttttagcaac acgagacatc ttgcattctc cttaggccac gtagcaaatc acttcgccac
      661 caacaccggt agcgcgcgct ttgcgatccg tcatcacgcc gcgcggagtg gaaacgattg
      721 ccacacccaa gccgttcatc acactaggaa tctcgtcttt ccccttatag atacgcagac
      781 cgggacggga cacacgctcg aggcgctcga tcacaggacg accagcgtag tacttcaagg
      841 cgattttcaa ttctgccttg ccgccggcct cagtcacggc gaaatcctca atatagcctt
      901 cgtccttgag aacggttgca atcgcgattt tgactctcga cgacggtatc actacagtcg
      961 tcttctgcac gccttgcgca ttgcggatac gggtcagcat atcggcgata ggatcgctca
     1021 tactcattgc ctgttctcct attaccagct ggctttggtc aaacccggaa tttcgccgcg
     1081 catggcgatt tcacggacct tgatacggcc caaaccgaat ttacggaatg tgccacgtgg
     1141 acgacctgtc aatgcacaac ggttacgttg gcgggtcgga gccgagttac gcggcagcgc
     1201 ctgcaacttc aggcgcgcct cgtaacgttc gccttccgac ctggattggt cgtcgataac
     1261 agccctcaac tcagcgcgct tgccggcgta ttttttcacc agatccgcac gcttttgctc
     1321 acggttaatc agtgccagtt ttgccatggc atcctcagtt tctgaacgga aatttaaatg
     1381 cggcgaggag cgctttcgct tcatcgtcgg tttttgcggt agtcgtgata ctgacattca
     1441 gaccacgcag cgcatcgatt ttatcgtatt caatttcagg aaaaatgatt tgctctttca
     1501 caccgatatt gtaattgccg cgaccatcga aagcgcgacc ggagacgcca cggaaatcac
     1561 gcacacgcgg caaagccacg gtaacaaagc gatccaggaa ctcatacatc tgcgcgccac
     1621 gcaaggtcac catgcagcca atcgggtaac cctcgcgaat cttgaaacca gcgattgcct
     1681 tgcgcgcctt ggtcactacc ggcttctgac cagcaatctt ggtcagatca ccgacagcat
     1741 gctcaatgat cttcttatcc gcgacagctt ccgatagacc catgttcaat gtaatcttca
     1801 gaatgcgcgg aacctccatt accgacttat aagcgtactt agcagtcaag tcagcaatga
     1861 ctttttcttt ataaaattct tggagacgtg ccatgatcta tcaaacctta acaacttcgc
     1921 cgctagattt ataaacacgg actttcttgc catccacact cttgtaagcc acgcggtccg
     1981 ccttgccgga agccgcattg aacaacgcaa cattcgacac atgaattggc atcagcttat
     2041 tgacgatgcc gccagttgca ccagtcattg gattaggccg gaccgctttt ttggcgacgt
     2101 tgacgccttc aacaacaaca taatcggcgt cgacacgctg agttaccaca ccacgcttgc
     2161 ccttgtcttt gccagtcagc acgatgactt catcattctt acgaattttt cccatgacta
     2221 ctccttacag aacttcaggc gccaaagaga cgatcttcat gaagcgctct gtacgcagct
     2281 cacgagtaac cggcccgaaa atacgagtac cgataggttc gagcttggcg ttcagcagaa
     2341 cagcagcatt gccatcgaat ttcaccaggg aaccatcctg acgacgcact cccttggcgg
     2401 tacggacgac gacagcgttg taaacttcac cttttttgac gcgaccacgt ggggcggcgc
     2461 tcttcacggt aaccttgatc acgtcaccaa tgcctgcata acgacgtctg gaaccaccca
     2521 gaaccttgat acacaaaact tcgcgcgcac cagtgttgtc ggccacctcg agccggcttt
     2581 cagtttgaat catgatattt tctttcccaa cttgatccgc tcaaatcaca caatgtgaat
     2641 cacggccagt tttggtcctg tcagcaggtg gacctgctgg ttaaggggac gataagctat
     2701 gtaaaacatc tgccttcgat ctctgccaaa ccaaatgcag aaattagctc agtattatga
     2761 cgtcataata ctgagcaagc aagcattatt taaacaagtt gcgccacttg tacaacgcgt
     2821 gtcacgctcc aggatttcgt cctggagatg ggacgacctt cctggatctc gacggtatcg
     2881 ccagtcttcg cctggttcat ctcatcatga gcgtggtatt tattggtacg cactataatt
     2941 ttgccgtaca acgggtgctt gacgtgacgc tcgattaaaa cggtaacagt cttatccatc
     3001 ttatcggaca caaccttgcc aatcagcgtg cgcttgagcg cttttttcac ttgatcgttc
     3061 attgttgtcc cttcgaattg atgactgttt tcacacgtgc gatatcacga cgtattttct
     3121 tcagttgcgc ggtattgttc aattgctgtg tggccacttg catgcgcaaa ctgaattgag
     3181 ctttcaacag ctcgttcagt tctttgaaca aatctgcttc gtccttacca cggagttcag
     3241 atgctttcat attccgttcc tgttattgac cgacttgacg aactacgaaa gtcgtcgaca
     3301 atggcagttt ggccgccgcc aggcggaacg cttcacgcgc caaagtttcg tcaacaccgt
     3361 ccatctcgta cagcatcttg cctggctgaa tctcggccac gtagtactcc ggattacctt
     3421 taccgttacc catacgcacc tcagcaggct tttgagaaat cggcttgtcc gggaaaatac
     3481 gaatccagat acgaccgcca cgcttgatgt gacgggtcat agcgcgacga gcggcttcga
     3541 tctgacgcgc cgtgatacgg ccacgaccaa ccgctttcaa accgaactca ccaaacgata
     3601 cgcttgtacc acgtgaatgc gagatacccg tattgcgacc tttttgttct ttacgatatt
     3661 ttctacgtgc tggttgcagc atgactattc tcctgctttc tcggccgata cagtgacatc
     3721 ggcagattga cgtggagcac gcttggtacc cacacctgct gctggagcgc ccccggtctt
     3781 cgaacgggga cgagcacccg gcttgccatc atcacggcgc ggaccacgac gcttcctgtc
     3841 atcttccgac tcaccgacca gcaccggcgc ttcgccgtta gccaaacgat cacctttgta
     3901 aacccatact ttgataccga tgatgccata ggtggtctgg gcttcactaa aaccgtagtc
     3961 gatatcggcg cgcagggtgt gcaaaggcac acggccttcg cgataccact cctgacgggc
     4021 aatttcgata ccgttcaaac gaccggacga catgatcttg ataccttggg cgcccagacg
     4081 cattgcatt
//
LOCUS       DASRDH010000035         6025 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_326188,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000035 DASRDH010000000
VERSION     DASRDH010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 6025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6025
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326188"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..935)
                     /locus_tag="VFA14_00870"
     CDS             complement(<1..935)
                     /locus_tag="VFA14_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012430097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HZS79975.1"
                     /translation="MKKNIKILRILPLYGPNIWTYRLALEVWIDIGELEDFPSNTIPG
                     FSERLMGWLPSLIEHRCSHGERGGFLRRLEEGTWPGHIMEHVTLELQNLAGLPGGFGK
                     ARETSVRGIYKVVVCAHHEQVTRAALHAGRDLVMAAIEDQPFDVDAVVAKLRSMADAL
                     CLGPSTACIVDAADERRIPSIRLSVGNLAQLGYGIRQRRIWTAETDRTSAIAARISRD
                     KDLTKTLLRSCGLAIPEGRIVNSPEDAWEAAIDIGLPVVVKPTDANHGRGVFIDLSTR
                     SEVETAYQAALEEGSSVLVERFIRGNEHRLLIVGG"
     gene            1249..3522
                     /locus_tag="VFA14_00875"
     CDS             1249..3522
                     /locus_tag="VFA14_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008328410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZS79976.1"
                     /translation="MNKKTLIPAHSGLPTHWRTQIQAQLAPDENILAWLELDLDITLR
                     FTPGLVLLTDRRLLAQDPSGNGWTGWEYHQGLVLKHDDHAGVGSLELQDSQARLACWR
                     YTLARHSQVLNLISRFVQQRDMRIFGATVAAEERKACSQCQAPLPHDRESCAICTETA
                     PPSSWALLRLTRFAGPYKGSLLLGFLLTLASTAATLVPPYMTMPLMDDVLIPFQNGAP
                     LNVRLTGFYLSGLLGAALVAWILGWGRTYILALVSERIGVDLRTTAYEHLLRLSQEYF
                     GGKRTGDLMARIGSETDRICVFLSLHLLDFIIDVLMIIMTVLILISIDPWLALVTLLP
                     LPFITWMIHLVRDRLRHGFEKIDRIWSEVTNVLADTIPGIRVVKAFAQEKREAARFRE
                     ANHRNLAVNDRVNKIWSLFTPTVTMLTETGLLVVWVFGIWLVSHHQITVGVLTAFLAY
                     ISRFYARLDSMSRIVSVTQKAAAGAKRIFDILDHVSSVPEPANPVHIAQVKGAIELRD
                     VGFRYGSRSVMRGVDLSIAPGEMIGLVGHSGSGKSTMVNLICRFYDVSEGSICIDGVD
                     IRSLPIAAYRRNIGLVLQEPFLFFGTIAENIAYGKPDASRAAIVAAARAAHAHEFILR
                     LPQAYDSLVGERGQALSGGERQRISIARALLIDPRLLILDEATSSVDTATEKEIQKAI
                     DNLVEGRTTIAIAHRLSTLRKADRLVVLDRGQVVEVGPHEELMAQRGTYYRLYQAQLR
                     QADSEAQSDDAAEKGEQ"
     gene            3519..4010
                     /locus_tag="VFA14_00880"
     CDS             3519..4010
                     /locus_tag="VFA14_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1854 domain-containing protein"
                     /protein_id="HZS79977.1"
                     /translation="MIPAQMNTPAHRFRLRHDALGRLVFIDADGSEHVGVVPVRAFPI
                     GAPDQGIALVSSDGHELAWIEKWPDVLPEIRAKIEAELALREFMPEIKHIVGVSSFAM
                     PSIWEVDTNRGATSFTLKGEEAIRRLPQSALLISDSHGIQFLIRDTRLLDRHSRRLLD
                     RFL"
     gene            4315..5349
                     /gene="mltB"
                     /locus_tag="VFA14_00885"
     CDS             4315..5349
                     /gene="mltB"
                     /locus_tag="VFA14_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007882158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HZS79978.1"
                     /translation="MPALIRAESTAARQKARAADAGEFASFNQWQEVNTFIDDMVARN
                     GFDKAALQTVFSKVRHLDTVIQLIRPASGDKPKNWRAYRARFVEPRRIDAGVDFWNQH
                     EADLSRAEVQYGVPAEIIVSIIGVETVYGRNTGDFRVMDAITTLAFDYPKSANHDART
                     AFFRKELESMLLLARESLIDPFSLSGSYAGAIGWPQFMPSSIRRYAIDFDGDGKIDLC
                     NSPVDAIGSIANFLKMHGWRYGAPTVFPATITDGSKIVKLIDQGLEAKFTPDELKAAG
                     IMPDAELPAGLRYGVVDLQNGAAPPEHWLATNNFFAITRYNRSYFYAMSVINLAHAVR
                     LKKAVDANHG"
     gene            complement(5383..>6025)
                     /locus_tag="VFA14_00890"
     CDS             complement(5383..>6025)
                     /locus_tag="VFA14_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011296562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015474 - autotransporter activity
                     [Evidence IEA];
                     GO_process: GO:0046819 - protein secretion by the type V
                     secretion system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HZS79979.1"
                     /translation="SSRAKVTGNAAQAKGEVEGWIAGVYGTWYQDDEKKLGAYVDTWL
                     QYGWFSNRVNGDQLPTVKYHTQGWAISGEAGYALPLGDDWVMEPQGQLLYVSYRKNGV
                     TESNGTQVLGEDSDGWTSRLGVRTYRTFVSEDFRKVQPYVTVNWWYAGENRKLSFDQT
                     PVDSPYASDRYELELGVSGEFDERWAGWSNVSGVWDGRGSYQYMLRLGVKYTW"
BASE COUNT         1230 a         1777 c         1715 g         1303 t
ORIGIN      
        1 ttgccgccga cgatcagtag acgatgttcg ttgccgcgga tgaaacgttc gaccagtacg
       61 ctgctgcctt cttccagcgc tgcctgatag gcggtttcaa cctcgctccg ggtgctgagg
      121 tcgataaaaa cgccgcggcc atgattggcg tcggtcggct tgacgaccac cggcaggcca
      181 atgtctatag cggcttccca ggcatcttcc ggactgttga cgatgcggcc ttcgggaatc
      241 gcgaggccgc aggatcgcaa cagggttttg gtgaggtctt tatcgcgcga gatgcgcgcg
      301 gcgatggcgc tggtccggtc ggtctcggcc gtccagatgc ggcgctgtct gataccatag
      361 ccgagctgcg cgagattacc tacagacagg cgaatcgacg gaatacgacg ctcgtcggca
      421 gcatcgacga tgcaggcggt actggggccg agacacagcg catcggccat gctgcgcagt
      481 tttgcaacga cggcgtcaac gtcgaagggc tgatcttcga tggctgccat gaccagatcg
      541 cggccagcat gcagggccgc gcgggtgacc tgttcgtggt gagcgcaaac cacgactttg
      601 taaatgccac gcaccgacgt ttcgcgggcc ttgccgaaac caccgggcag accggccaga
      661 ttttgcagtt ccagcgtgac atgttccatg atgtggccag gccaggtgcc ttcctcgagc
      721 cgtcgcagga aaccgccacg ttcgccatgg ctgcaacgat gttcgatcag ggaagggagc
      781 cagcccatca gccgctcact gaaaccggga atggtgttgg acggaaagtc ttccagctcc
      841 ccaatgtcga tccagacttc cagtgcaagc cggtaagtcc agatgttggg accatacaaa
      901 gggagaatgc gaagaatttt tatgtttttt ttcatgagta agcagtggtg ctgccgagtg
      961 ttgtccgttc cagcgatcca ggcatggttg acgcatgaaa cagcgatcgg ttcgtccgat
     1021 tacaaaaaaa tataatgtaa aaatttgaat tttatactga attttttgtt tttttatttc
     1081 ctgcagatga tctgtgtccg gcgacttgcc gccatcgtca aagcacggca gtgcgttatc
     1141 gggagcggca aatcagtacg atataatata cgcggcatgg attatcggca aaaaattgcc
     1201 agtaacatca ttcttcatgc tccatacagg gcatacatgc cgtcttcaat gaataaaaaa
     1261 accctcatcc cggctcattc cggtttaccc acccattggc gcacacaaat ccaggcccag
     1321 ctggcgccgg atgaaaacat ccttgcctgg ctcgaactgg atctcgatat cacgctgcga
     1381 tttacgcccg gactggtgct gctgacagat cgccgcctgc tggcgcaaga cccgtccggc
     1441 aatggctgga ccggctggga gtaccatcag gggctggtgc tgaaacatga tgatcatgct
     1501 ggtgtcggca gtctggagtt gcaggatagc caggcgcgcc tggcctgctg gcgttatacg
     1561 ctggcgcggc attcgcaggt tttgaacctg atcagccgat tcgtgcagca gcgtgatatg
     1621 cgcatctttg gcgcgaccgt ggccgcggag gagcgcaagg catgttcgca atgccaggcg
     1681 ccgcttcccc atgaccggga gagctgcgcc atctgcacgg aaacagcgcc gccgtcgtcc
     1741 tgggcgctgt tgcgtctgac gcgctttgcc gggccatata aaggctctct gctgctgggg
     1801 tttttgctga cgctggcatc gaccgccgcc acgctggtgc cgccgtacat gaccatgccg
     1861 ctgatggacg atgtgctgat tccattccag aacggcgcgc cgctgaatgt ccgcctgacc
     1921 ggcttctacc tgagcggctt gctgggtgcg gcgctggtgg cctggatact gggctgggga
     1981 cgtacttata ttctggcact ggtgagcgag cgcatcggcg tcgatttgcg cactaccgcc
     2041 tatgaacatc tgctgcgcct gtcgcaggaa tatttcggcg gcaagcgcac cggcgatctg
     2101 atggcgcgca tcggctcaga gaccgaccgg atttgcgtgt tcctgtcact gcatttgctg
     2161 gatttcatca ttgatgtcct gatgatcatc atgacggtgc tgatcctgat ttccatcgac
     2221 ccgtggctgg cgctggtgac actgttaccg ctgccgttca tcacctggat gattcatctg
     2281 gtgcgcgacc gcctgcgcca tggtttcgaa aaaattgatc gcatctggtc cgaggtgacc
     2341 aatgtgctgg ccgacacgat accgggaata cgcgtggtca aggcctttgc ccaggaaaag
     2401 cgcgaagcgg cgcgttttag ggaagccaat caccgtaatc tggccgtcaa cgaccgcgtc
     2461 aacaaaatct ggtcattgtt tacgccgaca gtcaccatgc tgaccgaaac tggtctgctg
     2521 gtggtgtggg tattcggtat ctggctggtc tcgcaccatc agatcacggt cggcgtgctg
     2581 acggcttttc tagcgtatat cagccgtttt tatgcacgtc tggattcgat gagccgtatc
     2641 gtgtcggtga cgcaaaaagc tgccgccggc gccaagcgca tcttcgatat cctcgaccac
     2701 gtttccagcg tgccggaacc agccaatccg gtgcatatcg cgcaggtgaa gggcgccatc
     2761 gaactgcgcg atgtgggctt tcgctatggt agccgttcgg tcatgcgcgg cgtcgacctg
     2821 tcgatagcgc cgggcgaaat gatcggtctg gtcgggcata gcggttccgg caagagtacc
     2881 atggtcaatc tgatctgccg tttttacgat gtatcggaag gctcgatctg catcgacggc
     2941 gtcgatatcc gttcgttgcc gattgccgca taccggcgca atatcggcct ggtattacag
     3001 gaacccttcc tgttcttcgg caccatcgcc gaaaacatcg cttacggcaa accggacgcc
     3061 agccgcgctg ccatcgtcgc ggcggcgcgc gcggcgcatg cgcatgaatt catcctgcgt
     3121 ctgccgcagg cctatgattc cctggtcggc gaacgcgggc aagctttgtc tggcggcgaa
     3181 cgtcaacgca tttcgatcgc acgcgccctg ctgatcgatc cgcgcctcct gattctcgat
     3241 gaggcgactt cttcggtgga taccgcaacc gagaaggaaa tccagaaagc catcgacaat
     3301 ctggtcgagg gccgtaccac gatcgccatt gcgcatcgcc tgtcgacctt gcgcaaggcc
     3361 gatcgcctcg tggtgctgga ccgcggtcag gtggtcgaag tcgggccgca tgaggaattg
     3421 atggcgcaac ggggaaccta ttatcggctg tatcaggcgc agttgcgtca agctgacagt
     3481 gaagcgcaaa gcgatgatgc cgcagaaaag ggcgagcaat gatacctgcg caaatgaaca
     3541 caccagcgca tcgcttccgc ctgcgacatg acgcgctggg gcgtctggtt ttcatcgatg
     3601 cggacggcag cgaacatgta ggggtggtcc cggtgcgagc ctttccgatc ggcgcgccgg
     3661 accagggtat cgcactggtc agcagcgacg gtcacgaact ggcgtggata gaaaaatggc
     3721 cagatgtttt gccggaaatc agagcgaaaa tcgaggctga gctggcgctg cgcgaattca
     3781 tgccggagat caagcacatc gtcggcgttt ccagcttcgc catgccttcc atctgggagg
     3841 tcgataccaa tcgcggcgcc acttcattca cgctcaaagg cgaagaggct atccgccgtc
     3901 tgccacaatc ggcgctcctg atttccgaca gccacggcat ccagtttctg atccgcgata
     3961 ccagattgct ggaccggcac agtcgcaggc tattggaccg ttttctgtaa tacaagcgtt
     4021 actgaacgca aaaaacatgc tgcacatcat cctgtaaccg atgttacagg gtgtaaaaac
     4081 gatggaaggg tcatcgcgga gccgatactc tggtagctcc ctccccctcc aatccatcag
     4141 cccccctgag gattggattt gacccgcgtg ccgcaaggtc cgcgggattt ttttgctgcg
     4201 tcagttggct cccaggcaac atcagctcat gtatactgtt gacacatgtc aataaaacat
     4261 tgcttccgtc tccccgcgct gtctggtctg ctcgtattgc tcgcggtttg tctgatgcct
     4321 gcactcatcc gtgcggaatc gacagcggcc agacagaagg cccgcgctgc cgacgctggt
     4381 gaattcgcca gcttcaacca atggcaggaa gtcaacacct tcatcgacga catggtcgcg
     4441 cgtaacggct tcgacaaggc agcgctgcaa acagtcttca gcaaggtgcg ccatctcgat
     4501 accgtgatcc agttgatcag gccggcatca ggagacaagc cgaaaaactg gcgagcctat
     4561 cgcgcgcgct tcgtcgaacc caggcgcatc gacgctggtg tcgacttctg gaaccagcat
     4621 gaagcggacc tgtcgcgcgc cgaggtgcaa tatggcgtac cggcagaaat catcgtcagc
     4681 atcattggtg tggaaaccgt ctatggccgc aataccggcg acttccgcgt gatggacgcg
     4741 atcaccacac tggctttcga ctatcccaag agcgccaatc atgatgcgcg taccgctttt
     4801 ttccgcaagg agctggaaag catgctgcta cttgcgcgtg aatcgcttat cgacccgttc
     4861 tcgctgtcag gctcctacgc cggcgccatc ggctggcctc aattcatgcc gagcagcatc
     4921 cgccggtatg ccattgattt cgatggcgat ggcaagatcg acttatgcaa ctcgccagtg
     4981 gatgccatcg gcagtattgc caattttctc aagatgcatg gctggcgata cggcgcgccg
     5041 acggtctttc ctgccaccat caccgatggc agcaaaatcg tcaagctgat cgatcaggga
     5101 ctggaagcca aatttacgcc ggacgaactc aaagccgccg gcatcatgcc ggatgccgaa
     5161 ctgcctgccg gcctgcgcta cggcgtagtc gacctgcaga acggcgcggc accgccggaa
     5221 cactggctgg cgaccaacaa cttcttcgcc atcacacgat acaaccgcag ctatttctat
     5281 gccatgtcag tgatcaatct tgcccacgcc gttcggctca agaaagcagt tgatgcaaat
     5341 cacggatgaa tccctgtttt tttccccgtt accgggatcc ggttaccagg tgtatttcac
     5401 acccaggcga agcatatact ggtaagaacc ccgcccatcc cagacgccgg agacattgga
     5461 ccagcccgcc cagcgctcgt cgaactcccc gctcacaccc aactcgagtt catagcggtc
     5521 cgatgcatac gggctgtcaa ccggcgtttg atcaaaggag agcttcctgt tctcacccgc
     5581 gtaccaccag ttgaccgtta cataaggttg tactttccgg aaatcctcgc tcacgaacgt
     5641 ccggtatgtg cgcacgccca gccggctcgt ccagccgtcc gagtcctccc cgagtacctg
     5701 tgtgccgttt gactcggtga cgccgttttt gcgatagctc acgtagagca gttgcccctg
     5761 cggttccata acccagtcat cgcccaaggg cagggcatag cccgcctcgc ccgagatggc
     5821 ccagccctgg gtatggtact tgacggtcgg aagctgatca ccgtttacgc gattactgaa
     5881 ccagccatac tggagccagg tatcgacata ggcgcccagt ttcttttcgt catcctgata
     5941 ccaggtgcca tagacgccgg ctatccaccc ctcgacctcg cccttggcct gcgcggcatt
     6001 acctgtgact ttcgcccgac tgctg
//
LOCUS       DASRDH010000036         2587 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1699936,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000036 DASRDH010000000
VERSION     DASRDH010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1699936"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1193)
                     /locus_tag="VFA14_00895"
     CDS             complement(<1..1193)
                     /locus_tag="VFA14_00895"
                     /inference="COORDINATES: protein motif:HMM:TIGR01760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HZS79980.1"
                     /translation="MVDKLTMRVVWDMLDKITKPLKAIKAQSGSTADALKKTRDELKR
                     LNIQQKQIGEFRELRNGLAQSAQRLKEAEDRVRELSLKIQSVQNPTRAMTREFERATK
                     AARAIKEENQKQSQQLTVLREKLEAAGISTGKGFGQQERRLRADIAAVNAQLAEQQQR
                     LAAVTRQQERMAAARHRLDGSRRFAGSVAGAGVSMIGAGNATGLPIVKTVRDFMTFED
                     SMLGVAKQVNGARDVNGKLTAIYYDMGKEIKRLATQIPMPTAEIADLVTAGARMGITG
                     DKQGEEAQQILLNFARDTAMMATAFEMPAGEIGDQMGKIAGIFKIDISSELSELGDSI
                     NYLDDNAISRGADIISVLHGDLAGAASTIGLRASHAAALASTLLTLGESPERADTAAS
                     GMLRQL"
     gene            complement(1186..1308)
                     /locus_tag="VFA14_00900"
     CDS             complement(1186..1308)
                     /locus_tag="VFA14_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003098394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HZS79981.1"
                     /translation="MADIAAVFHWSLSAMDDMSIPELMEWREQARLRCGAEKDG"
     gene            complement(1317..1658)
                     /locus_tag="VFA14_00905"
     CDS             complement(1317..1658)
                     /locus_tag="VFA14_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HZS79982.1"
                     /translation="MDKQQDEQIEAGKEINVNSRTITLDEPIERSGKDIRKITVRKPG
                     SGELRGVSLMALAEMDVTTLHRVLPRITSPILNELEVQKLAPADLMQFGVAVASFLLK
                     TQSRKEAFQSE"
     gene            complement(1719..2225)
                     /locus_tag="VFA14_00910"
     CDS             complement(1719..2225)
                     /locus_tag="VFA14_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008332385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HZS79983.1"
                     /translation="MSLPAKLKNFNLFNDGESFLGLVPELTLPKLSRKMEEYRAAGMT
                     GPIEVDFGNEAITLEWTAGGILKHALKQYGNTRHNGVQLRFAGAYQNDDTGAVTAVEI
                     VVRGRHKEIDFGNAKITEDTAHKFTTACSYYKLMIDGEDIIELDFINGTETIGGTDNS
                     AIRMALGL"
     gene            complement(2249..>2587)
                     /locus_tag="VFA14_00915"
     CDS             complement(2249..>2587)
                     /locus_tag="VFA14_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013232920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath C-terminal domain-containing
                     protein"
                     /protein_id="HZS79984.1"
                     /translation="GYFPFENYTRTAQILADTIAEAQFTIVDGPVTPGMIRDLLESIN
                     AKMRELKGRYIIAGKSWYDATKNDPTQLKDGKLTIDYDYTPVPPLENLMLNQRITDTY
                     LLDFASRIAV"
BASE COUNT          430 a          751 c          740 g          666 t
ORIGIN      
        1 agctggcgca gcatgccgga cgccgcggta tcagcacgct ccggcgattc gcccagcgtc
       61 agcagcgtag aggcgagcgc ggcggcatgc gacgcgcgca ggccgatggt ggaagctgcg
      121 ccagccagat cgccatgcag cacgctgatg atgtccgctc ccctggagat ggcgttgtcg
      181 tcgaggtagt tgatggagtc gccgagttcg cttaattcgc tgctgatgtc gatcttgaag
      241 atgccggcga tcttgcccat ctggtcgcca atttccccag caggcatttc gaacgccgtg
      301 gccatcattg ccgtatcgcg tgcgaagttg agcaggatct gctgggcttc ctcgccttgc
      361 ttgtcgcccg tgatgcccat gcgcgcgcca gcggtgacca gatcggcgat ttcggccgtc
      421 ggcatcggga tctgtgtggc gagacgtttg atctccttgc ccatgtcgta gtagatcgcg
      481 gtgagcttac cgttgacatc acgcgcaccg ttgacctgct tggcaacgcc gagcatgctg
      541 tcttcaaagg tcatgaaatc acgcaccgtc ttcacgatag gcagaccggt ggcattgcca
      601 gcgccgatca tgctgacacc agcgccggcg acactgccgg cgaaccggcg agatccgtcc
      661 agccgatgcc gtgctgctgc catgcgctcc tgttgccgcg taacggctgc cagcctctgc
      721 tgctgttctg ccagttgggc attaacggcc gcgatatcag cgcgcaggcg gcgttcctgc
      781 tggccgaacc ctttccccgt gctgataccg gcggcttcca gtttttcgcg caggaccgtc
      841 agctgctggc tttgtttctg attttcttcc ttgatggcgc gggcggcttt ggtggcgcgc
      901 tcgaattcgc gtgtcatggc acgtgtcggg ttttgtaccg actgtatttt cagcgacagt
      961 tccctgacgc gatcctcggc ttccttcaat ctctgtgcgc tttgtgcaag gccattgcgc
     1021 agttcccgga attcgccaat ctgcttttgc tggatgttca ggcgcttcag ttcatcgcgc
     1081 gtcttcttca gcgcatcggc ggtactgccg ctttgtgcct tgatcgcctt gagtggtttg
     1141 gtgatcttgt ccagcatgtc ccagaccact ctcatcgtca gtttgtcaac catccttttc
     1201 cgctccgcat cgcagacggg cctgttcccg ccattccatc aattcaggga tcgacatgtc
     1261 atccatcgcc gacagtgacc agtggaacac cgccgcgatg tcggccatcg cgttttctac
     1321 tcgctctgga aggcttcctt gcggctttgc gtcttcagca aaaaactggc caccgccacc
     1381 ccgaactgca tcagatctgc cggagccagc ttctgcactt ccagttcgtt caagatcgga
     1441 gaggtgatgc gcggcaacac gcggtgcagg gtggtgacat ccatttcggc caatgccatc
     1501 agcgacacgc cgcgcagttc gccggatcca ggcttgcgca ccgtgatctt cctgatgtcc
     1561 ttcccgctac gctcgatcgg ttcatcgagc gtaattgtgc ggctgttgac gttgatttct
     1621 ttccccgctt cgatctgttc atcttgttgc ttatccatgt taccgctcct gttccagttg
     1681 aaaaatggtc tgtcgttccg gacgtggtta atcggcgatc aaaggccaag tgccatgcga
     1741 atggcgctgt tgtcggtgcc gccgatggtt tccgtgccgt tgatgaagtc cagttcgatg
     1801 atgtcttcgc cgtcgatcat cagcttgtag tagctgcagg ctgtcgtgaa cttgtgggcc
     1861 gtgtcttcag tgattttggc attgccgaaa tcgatttctt tatgacgtcc gcgcacgacg
     1921 atttctactg ccgtcactgc accggtgtcg tcattctggt atgcgccggc aaaccgcagc
     1981 tgcacgccgt tgtgcctggt attgccgtac tgtttgagag catgtttgag gatgccgccg
     2041 gccgtccatt ccagcgttat ggcttcgttg ccgaaatcga cctcgattgg accagtcatg
     2101 ccggcagcgc gatactcttc catcttgcgc gagagtttcg gcagtgtcag ttccggaacc
     2161 aatccaagaa aagattcacc gtcgttgaaa agattgaagt tcttcagttt tgcaggcaat
     2221 gacatgtttt gctcctgttc atgtgttatc agacggcaat gcgcgaagcg aagtccagca
     2281 gataggtatc ggtgatgcgc tggttgagca tcaggttttc cagcggtggc accggcgtgt
     2341 agtcgtagtc gattgttagc ttgccgtcct tcagttgcgt cggatcgttc ttcgttgcgt
     2401 cgtaccatga cttgccggcg atgatgtagc ggcctttcag ttcgcgcatc ttggcgttga
     2461 tgctttccag cagatcacgg atcattcccg gcgtgacagg gccatcgaca atggtgaact
     2521 gcgcctcagc gatcgtgtcg gccagaatct gcgccgtgcg ggtatagttc tcgaaaggga
     2581 aatagcc
//
LOCUS       DASRDH010000037         3995 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1604846,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000037 DASRDH010000000
VERSION     DASRDH010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 3995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3995
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1604846"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="VFA14_00920"
     CDS             <1..273
                     /locus_tag="VFA14_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007877762.1"
                     /note="accepts sulfur from CsdA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HZS79985.1"
                     /translation="KVRKRLRARYQFPRGIKNRFGIDAVFSTEPLRVPEGEACEVNTV
                     DGRRQAGVTGLHCAGLGSVMVVTAAFGLVAAGFVLRKIVERPHRVV"
     gene            complement(329..1684)
                     /locus_tag="VFA14_00925"
     CDS             complement(329..1684)
                     /locus_tag="VFA14_00925"
                     /inference="COORDINATES: protein motif:HMM:NF009587.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctA"
                     /protein_id="HZS79986.1"
                     /translation="MNRFFKSLFGQVVVALMAGIIIGLLYPDFGQNLKPLGDGFIKLI
                     KMIIPVIVFCVVVQGICGTNDLKKVGSVGVKAIIYFEIVTTIALVLGLILAFIFQPGA
                     GMNIDPASLDVGGLSSYVETADKVKNSGFVDFVMKHIPGTVVSAFTSNDVLQVLLISV
                     IFGCALLLTGEKGKPTVILISSLSEIFFKCMSFIIRLTPLGVLGSIAFMVGRYGIGSL
                     KQLGMLVLLFYGTVLFFVIVVLGTILRASGLSIFRLIAYLREELIVVLATASADSVLP
                     QIMRKLEHMGIKQSTVGLVIPTGYSFNLDAFSIYLTMAVIFIAQATNTYLAMSDLLVI
                     LAITLITSKGAHGIPGSAIVILAATLTTIPTIPVVGLVLLLSIDWFVGIARALGNLLG
                     NCVATVVVATWEKDIDKTRACAVLNGEIPQVISNKRTNRASGWIEQNTEDAIHTVAQA
                     D"
     gene            complement(1884..3212)
                     /locus_tag="VFA14_00930"
     CDS             complement(1884..3212)
                     /locus_tag="VFA14_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006462269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZS79987.1"
                     /translation="MASTPIHHPETPGYSANTSVGSSDSHHPAVSPGDIAVGVVIGRA
                     SEYFDFFVYAIASVLVFPTVFFPFEDHLSGTLHAFVIFSFAFLARPFGTVIFMAIQKK
                     LGGEIKLTLALLLLGTSTAGIAFLPTYDHLGFTAIILLSILRIGQGIALGGSWDGLPS
                     LLAMNVPDNKRGWYAMLGQLGAPVGFMVAGLVFAYLVGSLSSADFLDWGWRYPFFVAF
                     AINVVALFARLRLISSEEYVQLLKERELEPTGLAELSVTQGRSIIIGAFIALASYVLF
                     HLVTVFPLSWISLYSLRSVSDFLMMQVFGAILMMLGIILSGPVADRYGRRITLSVLAV
                     LIAGLSGFVAQLINGGEVGQNVFILIGFTLLGLSYGQAAGAVSSNFSPKYRYTGAALT
                     SDLAWLVGAGFAPLVALGVSTQFGLAYVGLYLLSGAVCSLVALGASHALK"
     gene            3398..>3995
                     /gene="cyoA"
                     /locus_tag="VFA14_00935"
     CDS             3398..>3995
                     /gene="cyoA"
                     /locus_tag="VFA14_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013234442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol oxidase subunit II"
                     /protein_id="HZS79988.1"
                     /translation="MPSHLIRRGLIFLSVVFLAGCNTVVLNPSGDVAQQQGRLIVVST
                     VLMLLVIVPVIALTVLYAWHYRQSNTAARYEPDWAHSTQLELIIWGAPLLIIIALGLV
                     TWVTTHTLDPYRKLSRIDAQRPILEHIKPLTVEVVALDWKWLFIYPEQGIATVNELAA
                     PVNVPINFKITASSFMNSFYIPALAGQIYAMPSMQTALN"
BASE COUNT          961 a         1053 c         1117 g          864 t
ORIGIN      
        1 aaagtgcgta agcgtttgcg tgcccggtat cagtttccac gtgggatcaa gaacaggttc
       61 ggtattgatg ccgtgttttc tactgaaccg ttgcgcgttc ctgaagggga agcttgcgag
      121 gtgaatactg tcgatgggcg gcggcaggct ggcgttaccg gcttgcattg tgctgggctg
      181 gggtcggtga tggtggtgac agctgcgttc gggctggtgg cggcggggtt tgtgttacgg
      241 aagatcgttg aacgtccaca tagggtagtg taactgtttg gcagagaggc cgggggtact
      301 gcgctggtca tgggatcaac agtttttttc agtctgcttg ggcaacagtg tgaattgcat
      361 cctctgtatt ttgctctatc catccacttg ccctgttagt ccgtttgttc gaaatcacct
      421 gtgggatttc tccattgagc acggcgcaag cacgggtctt gtcgatgtct ttttcccatg
      481 tggcgacgac tacggtagcg acgcagttgc cgagcaggtt gcctagcgcg cgggcgattc
      541 ctacaaacca gtcgatcgac agcagcagta ccagaccgac tactggaatg gtcggaatcg
      601 tcgtcagtgt cgctgccagg atcacaatgg cagaacctgg tatgccgtgc gcccccttag
      661 aggtaatcag cgtaattgcc agaatcacca gcaggtcgct cattgccaga tacgtattgg
      721 tggcttgggc gatgaatatt accgccatgg tcagatagat cgaaaaggcg tcgaggttga
      781 aggaataacc tgtcgggatc accagaccga cggtcgactg cttgatgccc atgtgttcga
      841 gtttgcgcat gatttgcggc aacacactgt cggctgaggc agtcgccagc accacgatca
      901 gttcttcgcg tagataagcg atcagcctaa agatgctcaa tcctgaggcg cgcaggattg
      961 tacccaatac cactatcacg aagaacagta cggtgccgta aaacagcagc accagcatgc
     1021 ccagctgctt gagcgaaccg atgccatagc ggccgaccat gaaggcgatt gagccgagaa
     1081 cgcccaatgg cgtcaggcgg atgatgaagg acatgcactt gaaaaaaatt tccgacagcg
     1141 aagaaatcag gatcacggtc ggtttgcctt tttcgccggt cagcaataag gcgcaaccga
     1201 agatgacgga aatcagcaag acctgcagca cgtcgttcga ggtgaaggcg ctcacgaccg
     1261 tgccaggaat gtgcttcatg acgaagtcga cgaagccgct gtttttgact ttgtcggcag
     1321 tttcgacata gctggataag ccgcctacat cgagactagc cggatcgatg ttcatgccgg
     1381 cacccggctg gaaaatgaag gcgagtatca gtccgagtac gagcgcaatc gtcgtcacga
     1441 tctcaaaata aataatggcc ttgacaccga cgctgccgac tttcttcaaa tcgttggtgc
     1501 cgcagatgcc ttgcacgact acgcagaaca cgatcaccgg aatgatcatc ttgatgagtt
     1561 tgataaaccc gtcacccagt ggcttgagat tttgcccgaa atcgggatag agcagtccga
     1621 taatgatgcc ggccatgagg gcgacgacga cttgcccgaa caaagatttg aaaaatcggt
     1681 tcatcttgat cttttgatgt cgttctggat gagcgttgtc gtgtctgctt tccaacaata
     1741 aaaacctgcg gtcatcatga ctgtccgcga tggcggcaaa taaaaaggct gagtctgtgc
     1801 aatacagaac tcagccttta ctgccggtcc ggttgcccac actgaaccgg ccaatttcat
     1861 ggggagacag ttcagctaca cgttcatttc aatgcatggc tcgcaccaag cgccaccagt
     1921 gagcagactg cgcccgacaa cagatagagg ccgacatagg ccagaccgaa ttgcgtcgat
     1981 actcccaagg ctaccagcgg ggcaaagcct gcgccgacca gccatgccag gtcggaggtc
     2041 agcgccgcac cggtatagcg gtacttggga gaaaaattgg agcttacagc gccagctgcc
     2101 tgaccatacg acaagcccag cagagtgaag ccgatcagga tgaaaacatt ctggccgact
     2161 tcgccgccgt tgatcagctg tgccacgaag ccgctcaggc cagcaatcag cactgccagc
     2221 acacttagtg tgatgcggcg gccgtagcgg tcggcgaccg ggccggacag gataattcca
     2281 agcatcatca atatagcgcc gaacacctgc atcatcagaa aatcactgac agagcgcagc
     2341 gaatacagcg aaatccaaga cagcggaaat accgtcacca gatggaatag cacatagctg
     2401 gccagcgcga tgaaggcacc gatgatgatg ctgcgacctt gtgtgacgga gagttcggcg
     2461 aggccggtcg gttccagctc acgttctttc agcaattgca catattcttc ggaggaaatc
     2521 aggcgcaagc gggcaaacag ggcgacgacg ttgatggcga acgccacaaa gaacggatag
     2581 cgccagcccc aatcgaggaa atctgcgctg ctcaggctgc cgaccaggta agcgaacacc
     2641 agaccggcga ccatgaagcc gaccggcgca cctaattgac cgagcatggc gtaccagccg
     2701 cgcttgttat caggtacgtt catcgccagc agggagggca gaccgtccca ggagccgccc
     2761 agcgctatac cctggccgat gcgcagtatc gacagcagga tgatggccgt gaaacccaga
     2821 tgatcgtaag ttggtaaaaa ggcaatgccg gcagtggatg tgccgagcag gagcagggcc
     2881 agcgtcagct tgatttcacc ccccagcttt ttttgtatcg ccatgaaaat aacggttccg
     2941 aacggtcgcg ctagaaaagc gaaggaaaaa atgacgaagg catggagcgt gccgctgagg
     3001 tggtcttcga acggaaaaaa aactgtggga aaaaccaaca ccgaggctat ggcatatacg
     3061 aagaaatcaa agtattctga agctcttcca atgaccacgc cgaccgcaat gtctcccggc
     3121 gagaccgctg gatgatgaga atcagaacta ccgacagacg tgttcgcaga atatccagga
     3181 gtttcagggt gatgaatagg ggtgctagcc atgatcatta tctctctttc tcgattcagt
     3241 ctgtgacgcc gtctacgttc catcggagca tgatcgatta tactgctgtt ctattgctgt
     3301 ggttccgttg tcagggtagg acaaattgtc caatcgctaa tgcttaacgg tgagtgtaca
     3361 ttaacagaat ccgatagttc ccactggact ttccagcatg ccttcacatc tcattcgccg
     3421 cggactcatt ttcctgtctg tcgttttcct ggcaggttgt aatacggtcg tcctgaatcc
     3481 atcgggagac gtcgcccagc aacagggcag gctgatcgtt gtttccaccg tcttgatgtt
     3541 gctcgtcatc gttccggtga ttgcgctgac ggtcctttat gcatggcatt accgtcagag
     3601 caatactgcg gcccgctatg agccggactg ggcccactcg acccaactcg aactgattat
     3661 ttggggggct ccgctgctga tcatcattgc gctgggcctg gtgacctggg tcactaccca
     3721 cacgctggat ccctatcgca agctgtcgcg tatcgatgcg cagcgtccca ttctcgagca
     3781 catcaaaccg ctgactgtcg aagtcgtggc gctggactgg aagtggctgt tcatctatcc
     3841 ggagcaaggc attgccactg tcaatgaact ggcagcgccg gtcaacgtgc cgatcaattt
     3901 caagatcact gcgtccagct tcatgaactc tttctatatt cctgcgctgg ctggccagat
     3961 ttacgccatg cccagcatgc agacagcgct gaacg
//
LOCUS       DASRDH010000038         6021 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1128050,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000038 DASRDH010000000
VERSION     DASRDH010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 6021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6021
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1128050"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..171)
                     /locus_tag="VFA14_00940"
     CDS             complement(<1..171)
                     /locus_tag="VFA14_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003820514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HZS79989.1"
                     /translation="MLENAGNPLSDAVESTPDMVDGCAFARLYGEPLFKLPADLYIPP
                     DALEVFLEAFQGP"
     gene            complement(181..387)
                     /locus_tag="VFA14_00945"
     CDS             complement(181..387)
                     /locus_tag="VFA14_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016836435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3460 family protein"
                     /protein_id="HZS79990.1"
                     /translation="MFFSKFFTSYESEVTRFITELKQKHPQLEEQQRAGRALLWDKAQ
                     LDLDKTARDEESRVRQQPYVYQSY"
     gene            784..1116
                     /locus_tag="VFA14_00950"
     CDS             784..1116
                     /locus_tag="VFA14_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS79991.1"
                     /translation="MTIPNDTNNSSLSQPTYDPNHLLNALIKKLHLKNDAALSRALEV
                     APPVISKIRHRRLPIGASLLIRMHEVSDLSIRNLRELMGDRRAKFRISDKQFKPKQGA
                     SAKMEAQP"
     gene            complement(1167..2069)
                     /gene="cysM"
                     /locus_tag="VFA14_00955"
     CDS             complement(1167..2069)
                     /gene="cysM"
                     /locus_tag="VFA14_00955"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005669006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase CysM"
                     /protein_id="HZS79992.1"
                     /translation="MPYLTIEDTIGNTPLIQLRRIGGEDVVRRNNVILGKMEGNNPAG
                     SVKDRAALLMIRRAEERGQIRPGDTLIEATSGNTGIALAMVAAMRGYKMVLLMPENLS
                     EERRQSMAVYGATIILTPQSGGMEYARDLAQQMQKEGQGLILDQFANSDNSLAHYQTT
                     GPELWRDTEGRITHFISAMGTTGTIMGVSRYLKEQNPEIQIIGAQPEEGSSIPGIRKW
                     PEEYLPKIYEKSRVDRLEYISQAEAENMARKLAVAEGLFCGISAAGACEVALRVSQQL
                     ENATIVFVVCDRGDRYLSTGVFPA"
     gene            complement(2210..2377)
                     /gene="rpmG"
                     /locus_tag="VFA14_00960"
     CDS             complement(2210..2377)
                     /gene="rpmG"
                     /locus_tag="VFA14_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HZS79993.1"
                     /translation="MAKSGRDKIKLESTAGTGHFYTTTKNKRTTPEKLAITKFDPKAR
                     KHVEYKETKIK"
     gene            complement(2402..2638)
                     /gene="rpmB"
                     /locus_tag="VFA14_00965"
     CDS             complement(2402..2638)
                     /gene="rpmB"
                     /locus_tag="VFA14_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008451488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HZS79994.1"
                     /translation="MARVCQVTGKGPMVGNNVSHANNKTKRRFLPNLQNRRIFVESEN
                     RWVSLRLSNAGLRVIDKIGIDAVLADMRARGEKV"
     gene            complement(2816..3490)
                     /gene="radC"
                     /locus_tag="VFA14_00970"
     CDS             complement(2816..3490)
                     /gene="radC"
                     /locus_tag="VFA14_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HZS79995.1"
                     /translation="MAITDWPQGQRPRERLIDQGAHALSNAELLAILLRVGVAGKSAV
                     DLGRDMVGHFGSLQGLFSARLADFVRINGLGPAKYAQLQAVLELGRRVLSEELRAGTS
                     LGSPKAVKEYLQLLFHGKPHEAFLVMFLDVKNRLIYSEELFRGTLTHASVYPREIVKA
                     ALLHNAAKVILAHNHPSGEVTPSEADLHLTSHIKRALALIEVQVVDHIVVGGRRTHSF
                     AEHRQI"
     gene            3608..4063
                     /locus_tag="VFA14_00975"
     CDS             3608..4063
                     /locus_tag="VFA14_00975"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008114365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HZS79996.1"
                     /translation="MSSISVPIVTENAYLTLHYRLASQAGENIVSTFEGNPATLQFGQ
                     GQLAPFLEAFLLGLPEGAHRTFELTPERTFGPRKPELIQRIARATLEENSPLDEKYRI
                     GDLVDFTAPGGGRFAGVLREIDAQSALFDFNHPLSGKSLKFEVKIIGVL"
     gene            4074..5033
                     /gene="ispH"
                     /locus_tag="VFA14_00980"
                     /gene_synonym="lytB"
     CDS             4074..5033
                     /gene="ispH"
                     /locus_tag="VFA14_00980"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HZS79997.1"
                     /translation="MNNNEPEILLAQPRGFCAGVNRAIEIVERALTQLGAPIYVRHAI
                     VHNAYVVADLRSKGAIFVEELADVPAGNTVVFSAHGVSKAVQAEALERGLSVFDATCP
                     LVTKVHMEVNKMRREGREIIMIGHAGHPEVEGIMGQTDTGIYLVETVDDVLQLDVTDS
                     DLLAYVSQTTLSVDDTADIIAALQKRFPHICAPKKSDICYATTNRQEALKFMAPQVDM
                     IIVVGSPNSSNSNRLREVAEKKGVPAYMVDNADLIDADWLVGKRRIGVTAGASAPEVL
                     VQAVIDKLKSGTKRSVRILDGVKENVTFPLPKGLGDVHQDVSP"
     gene            5030..>6021
                     /gene="dapC"
                     /locus_tag="VFA14_00985"
     CDS             5030..>6021
                     /gene="dapC"
                     /locus_tag="VFA14_00985"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009016 - succinyldiaminopimelate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019877 - diaminopimelate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HZS79998.1"
                     /translation="MNPLLDKLQPYPFEKLKNLFSGVTPHPGYRHISLGIGEPQHPTP
                     AFIQQALTDNLAGLASYPGTIGTDALRSAIAGWLERRYDLPDIDPATQVLPVNGSREA
                     LFSLTQTVIDPAQKDALVMCPNPFYQIYEGAAYLAGAQPYFVNSDPARNFAPDFNAVP
                     DHVWARVRLLFLCSPGNPTGAVLSLGDWQQLFALSDRHGFVIAADECYSEIYFRDEPP
                     LGGLEAADQLGRKDFKNLIALTSLSKRSNVPGLRSGFVAGDAAVIKSFLLYRTYHGSA
                     MSGSVAAASIAAWKDESHVVDNRAQYRAKFAAVTPLLEPVLDVRLPDASFYLWAG"
BASE COUNT         1357 a         1658 c         1603 g         1403 t
ORIGIN      
        1 cggcccctgg aaggcttcca gaaacacctc cagcgcatca ggagggatgt ataggtcggc
       61 cggcagcttg aacagcggct cgccatacag cctagcgaag gcgcagccat caaccatatc
      121 cggcgtcgat tcaacggcat cggagagtgg atttccggca ttttcgagca tgctgatgga
      181 ttagtagctc tggtagacgt atggctgctg cctgacgcgc gactcctcgt cgcgcgcagt
      241 cttgtccagg tccagctgcg ccttgtccca caacagggcg cgaccggcgc gttgctgctc
      301 ttccagttgc ggatgctttt gtttgagttc agtgatgaat ctggtgactt ccgactcgta
      361 ggaggtgaaa aatttgctga aaaacatgat ttatcctgga aacccctgaa tttcccatca
      421 aatggcgaat aggctaggca ctattttacc ctgtcctgcg gcgtgaatag caaaaatgac
      481 aatgatcaga aagcctggct cttttgtcgc gccaaagaaa caacaagaaa aatatagaaa
      541 aaaatcctgt taatatcaag taaaaacatt tggttataat tacaactatt ttgtaataaa
      601 tttttatcaa tgtagatatt gttactcgtc aagtattgat attacatcaa tattgcagtg
      661 gggaaaaatg acggcgtctt taatggaaaa cccgttaacc acgttttgca gttttaccag
      721 gaacgaatag cgcttgcgca aacaagaccc ttataaagca cctgcataat ggaacgtccg
      781 atcatgacta tacctaatga caccaacaat tcttctctgt ctcagcccac ttacgatccc
      841 aatcatctgc tcaacgcgct gatcaaaaaa ctgcatctga agaatgatgc tgcgctatcg
      901 cgcgcccttg aagtagctcc tccggtcatc agcaaaattc gccaccgccg cttgccgatt
      961 ggtgcttcgc tactaatccg catgcatgaa gtgagtgacc tgtcaatccg taatctgcgc
     1021 gaactgatgg gagatcgccg cgcaaaattc cgcatcagcg acaagcaatt caaaccaaaa
     1081 caaggcgcat cggccaaaat ggaggcacaa ccatgatgcc gctcattggc tgaaacagtg
     1141 ctttcgtggt gacatcacgt tgttgatcag gccgggaaaa cgccggtgga cagatagcgg
     1201 tcgccacggt cgcagaccac gaatacgata gttgcatttt caagctgctg cgaaacgcgc
     1261 aaagccactt cgcaagcgcc cgctgccgaa ataccgcaaa acaagccttc cgctactgcc
     1321 agtttgcgcg ccatgttttc ggcctcggcc tggctgatgt attccaggcg atcgacacgt
     1381 gatttttcgt agatcttcgg cagatactct tccggccatt tgcggatgcc gggaatggac
     1441 gagccctctt ccggctgggc accaatgatc tggatctcag ggttctgttc tttcagatag
     1501 cgcgacacgc ccatgatggt gccggtggtg cccatggcgc tgatgaaatg ggtgatgcgg
     1561 ccttccgtgt cgcgccatag ttcgggaccg gtagtctggt aatgcgccaa cgaattatca
     1621 gaattggcga actggtcgag aatcagcccc tggccttcct tttgcatctg ctgggcgaga
     1681 tcacgcgcgt attccatacc cccgctctgt ggcgtgagga tgatcgtggc gccgtagaca
     1741 gccatgctct ggcgacgctc ctcactgagg ttttccggca tcaacaaaac catcttgtag
     1801 ccgcgcattg cggctaccat ggccagtgcg ataccggtat tgccgctggt ggcttcgatc
     1861 agggtatcgc ccggtctgat ctggccgcgc tcttcagcgc ggcgaatcat cagcagtgca
     1921 gcgcggtctt tgacggaacc ggcagggttg ttaccctcca ttttgccgag gataacattg
     1981 ttgcgccgca caacgtcttc gccaccaatg cgtcgcagtt gaatcaaggg ggtattaccg
     2041 atagtatctt cgatggtcag gtaaggcatg atcagtgtaa tcctttcaac aatccagcct
     2101 gaaccgtacg tatattttac caataaactc cctccgaaag tcgatacccg actttcggag
     2161 gccattcagt gttgcgttgc ggaatttttt atgacaggaa aaaaaccagt tacttgatct
     2221 tggtttcctt gtattcgacg tgcttgcgag ccttgggatc gaacttggtg atcgccagct
     2281 tctcaggtgt cgtgcgcttg ttttttgttg tcgtgtagaa gtgacctgta cctgcggtcg
     2341 actccagctt gattttgtcg cggccggatt tagccatgat ggatttcctt tactctgctg
     2401 gttagacttt ttcgccacgg gcgcgcatgt cggccaaaac ggcatcgatg ccaattttgt
     2461 cgatcacgcg caaaccggca ttggacaagc gcagggaaac ccagcggttt tccgactcga
     2521 cgaaaatacg gcggttttgc aggttaggca aaaaacgacg tttggtcttg ttattcgcat
     2581 gggaaacgtt gttgccgacc atcggccctt tcccggtgac ttggcaaaca cgtgccatgt
     2641 tgatgctcct taaaaattcc agaaattgga agatggggaa ttttatagag aaaactctga
     2701 tttttcaatt acttgcaaaa aacaattgtg tcttcatctc ttttttttgt tttacattta
     2761 tttcgtccga aaaaccgtgt ctgcctgtcc tgaacgcgga caggatggac gtttttcaga
     2821 tctggcggtg ttctgcaaag gaatgcgtgc gtcgcccgcc cacgacgatg tgatcgacga
     2881 cctggacttc gatcagtgcc agcgcccgct tgatatgact ggtcagatga agatccgctt
     2941 cactgggagt gacttcgccg gaagggtgat tgtgcgctag gatgaccttg gcggcattat
     3001 gaagcagtgc ggccttgacg atttcgcgcg gatacacgct ggcatgggtc aaggttccac
     3061 gaaacaattc ttcgctgtat atcaggcgat tcttgacgtc gagaaacatc acgaggaatg
     3121 cctcatgagg cttgccgtga aacagcagct gcaggtactc cttgaccgcc ttcggagatc
     3181 ccagcgacgt cccggcgcgc agttcttccg acaaaacacg ccgccccagt tccagtactg
     3241 cctgtaattg cgcatatttg gcagggccca ggccattgat gcgtacgaaa tcagccagtc
     3301 gggcagaaaa cagtccttgt agcgaaccga aatgcccgac catgtcgcgc cccaggtcga
     3361 ctgcgctttt gccggcgaca ccgacgcgca gaagtatcgc cagtaactcg gcatttgata
     3421 gcgcatgggc gccttgatca atcaggcgct cgcgtgggcg ctggccttgt ggccaatcag
     3481 taattgccat gttgtcctct ttgatgatgg tgatgaaatc tgtttcctat tctgctgcaa
     3541 ggccataacc cggtgccgag caaggtgact tacaatgaca ggctgtcacg cttactgaag
     3601 atttgtcatg tccagcatct ctgtccccat cgtcactgaa aacgcctacc ttacgctgca
     3661 ctaccgcctg gcatcgcaag ccggtgaaaa tatcgtcagc acttttgagg gaaatcccgc
     3721 taccttgcaa tttggacaag gacaactggc tccatttctg gaagccttct tgctggggtt
     3781 gccggaagga gcccatcgga cgttcgagct gacaccagaa agaacgtttg gaccacgcaa
     3841 gcctgaattg attcagcgca tcgcgcgcgc gaccctggaa gaaaattcgc cgctggatga
     3901 aaaataccgt attggcgacc tggtcgattt caccgcacca ggcggcgggc gctttgccgg
     3961 cgtgctgcgc gagatcgacg cacagagcgc gctgtttgac ttcaaccatc cattgtctgg
     4021 aaaatcactg aaattcgaag tgaaaatcat tggggttcta taatattttt gatatgaata
     4081 ataatgagcc agaaatttta ttggcccaac cacgtggttt ctgtgctggg gtcaatcgtg
     4141 ccattgaaat cgtcgagcgc gcgctgacgc agttaggtgc gccgatctat gtgcgtcatg
     4201 caatcgtcca taatgcttat gtcgttgccg acctgcgcag caagggcgcg attttcgtcg
     4261 aggagttagc cgatgtgccg gccgggaaca ccgtcgtttt ttccgcgcac ggcgtttcca
     4321 aagccgtgca ggcagaagcg ctggagcgtg gcctgagcgt tttcgatgcc acctgtccat
     4381 tggttaccaa agtccatatg gaagtgaata aaatgcgccg tgaaggccgt gaaatcatca
     4441 tgatcgggca cgcaggccat cctgaagtcg aagggatcat ggggcagacc gataccggca
     4501 tctatttggt cgaaactgtc gacgatgtat tacagctcga tgtcactgat tccgatctgc
     4561 tggcctatgt gtcgcaaacc acgctatcgg tggacgatac ggccgacatc atcgcagcac
     4621 tccagaaaag atttccccat atctgcgcac cgaagaaaag cgatatctgc tacgccacca
     4681 ccaaccgcca ggaggcgctt aaattcatgg cgccgcaggt cgatatgatc atcgtagtcg
     4741 gcagtccgaa cagttccaat tccaatcgct tgcgtgaagt cgcggaaaag aaaggtgttc
     4801 ctgcctacat ggtcgacaac gccgatctga tcgatgccga ctggttggtt ggcaagcgac
     4861 gcattggtgt gacagcgggg gcgtcggctc ccgaagtact ggtgcaggcc gtcatcgata
     4921 agctcaagag tggtacgaaa cgcagtgttc gcattctgga cggtgtcaaa gaaaacgtca
     4981 cttttccgct gccaaagggt ttgggcgacg tacaccagga tgtttcccca tgaatccctt
     5041 actcgacaag ctgcaaccct acccgttcga aaaactgaaa aatctgtttt ccggcgtaac
     5101 gccccatccc ggttatcgcc acatcagcct gggcatcggt gaaccgcagc acccgactcc
     5161 ggccttcatc cagcaagcgc tgaccgacaa tcttgccggc ctggcgtcct atcccggcac
     5221 catcggcacg gacgctttgc gcagcgctat cgccggctgg ctggagcgcc gctacgacct
     5281 gcctgacatc gatccggcaa cacaggtgct gccggtcaat ggttcgcgcg aggccctgtt
     5341 ttcgctgacg caaaccgtca tcgatccagc gcaaaaagat gcgctggtga tgtgtccgaa
     5401 tccgttctac caaatctatg aaggcgcggc ctacctggcg ggcgcgcagc cttacttcgt
     5461 caattccgat ccggcacgca attttgcacc ggacttcaac gctgtgccgg atcacgtctg
     5521 ggcgcgcgtc agactgctgt ttctatgctc accgggcaat ccgaccggcg ccgtgttatc
     5581 gctgggagac tggcaacaac tgttcgcgct gtccgaccgc catggtttcg tgatcgcggc
     5641 cgacgagtgc tacagcgaaa tctatttccg cgacgagccg ccgctggggg gcctggaggc
     5701 ggccgaccaa ctgggccgca aggatttcaa aaacctgatc gcgctcacct cgctgtcgaa
     5761 gcgcagcaac gtgccgggcc tgcgcagcgg cttcgtggcc ggcgacgccg ccgtcatcaa
     5821 gagcttcctg ctctaccgca cctaccacgg cagcgcgatg agtggcagcg tcgcggcagc
     5881 cagcatcgcg gcctggaaag acgagtcgca cgtggtggac aaccgcgccc agtaccgcgc
     5941 caagttcgcc gccgtgacgc cgctgctcga gccggtgctc gacgtccggc tgcccgacgc
     6001 cagcttctac ctctgggccg g
//
LOCUS       DASRDH010000039         7617 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1475520,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000039 DASRDH010000000
VERSION     DASRDH010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 7617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7617
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1475520"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /locus_tag="VFA14_00990"
     CDS             <1..401
                     /locus_tag="VFA14_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HZS79999.1"
                     /translation="ASAVLAITLNSGAYVSEIFRAGIQSIDRGQSEAARSLGMSYMQT
                     LRKVVLPQAFRRMLPPLGNNAIAIVKDSSLASAIGLAELAYAARTVSGAYARYWEPYL
                     TISLIYWVITLLLSIFVRHLENRYGKGDVR"
     gene            398..1120
                     /locus_tag="VFA14_00995"
     CDS             398..1120
                     /locus_tag="VFA14_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013232150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HZS80000.1"
                     /translation="MIHVKQLQKQYGDAHILRGIDCDIEESEVVCVIGPSGSGKSTFL
                     RCLNGLEEATDGEIVIDGVKVNDPRVSLDTLRAGMGMVFQRFNLFPHMPVLENLIMAP
                     MQVKKLSRREAVLVAEKLLQKVGLLDKIDAFPNQLSGGQQQRVAIARALAMAPKVMLF
                     DEPTSALDPELVGEVLTVMKTLAEEGMTMVVVTHEMGFAREVSDRVFFIDQGVIMEEG
                     PPQQVLGEPRNERTRDFLRKVL"
     gene            complement(1224..2258)
                     /locus_tag="VFA14_01000"
     CDS             complement(1224..2258)
                     /locus_tag="VFA14_01000"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007882336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HZS80001.1"
                     /translation="MFDKFLYAFARPLLFSLDAESAHHLTLSALRRAAALGMTGCISK
                     PAADPRTVMGITFPNSVGLAAGLDKDGSYIDGLAALGFGFIEIGTVTPHAQPGNPKPR
                     MFRLPAAHAIINRMGFNNGGVDAFVANIQASRFYQSKQGVLGLNIGKNADQPIERAAA
                     DYLYCLEKVYPYAGYVTINISSPNTRNLRQLQGASELDALLSQLKEAQQRLADRYKRY
                     VPLALKIAPDIDSEQIKNIADALLRHKLDGVIATNTTITRDAVKGMLHGEETGGLSGA
                     PVFELSNQVVRGLKAELGDALPIIGVGGILSGSNAKAKMQAGASLVQLYSGLIYRGPA
                     LVKECADALR"
     gene            complement(2329..3057)
                     /locus_tag="VFA14_01005"
     CDS             complement(2329..3057)
                     /locus_tag="VFA14_01005"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyl-tRNA--protein
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HZS80002.1"
                     /translation="MMHGKDLPFSALQFYATASYPCSYLDNQQARSQVAAPSHLINAD
                     VYAELVKSGFRRSGIFTYRPYCDGCRACTPVRINAAEFTPNRSQRRAWARNRQLVTLV
                     TNLAYACEHYELYLRYQSVRHAGSGMDQDSREQYTQFLLQSRVNTRLVEFRDSSGVLR
                     MVSIIDVLDDGLSSVYTFYDPDSESASYGTYNILWQIEQTCQLGMSYVYLGYWIKESA
                     KMAYKINFKPLEALRGSNWIALDE"
     gene            complement(3054..3788)
                     /gene="aat"
                     /locus_tag="VFA14_01010"
     CDS             complement(3054..3788)
                     /gene="aat"
                     /locus_tag="VFA14_01010"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HZS80003.1"
                     /translation="MISWLDVDSPFPDVSRALADPAGLLAVGADLSPQRLLDAYRHGI
                     FPWFSEGQPILWWSTDPRMVLYTDRLIVSDSLKKTLRKVERSRHTDARWNVRFDSAFR
                     DVMQACAEPRKETAGTWISRGIVDSYCHLHQAGYAHSSEVWLEGRLVGGAYGVSIGRM
                     FYGESMFARVSDASKVALTYLVHFLRQHGVRMIDCQQETSHLASLGAVPIPRSAFLAH
                     LRQSIDAPQITCWHPPDLVTARQEDA"
     gene            complement(3804..4379)
                     /locus_tag="VFA14_01015"
     CDS             complement(3804..4379)
                     /locus_tag="VFA14_01015"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007882331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HZS80004.1"
                     /translation="MKFCSECAHPVSLRIPPDDNRPRFVCGHCGAIHYQNPKMVVGSI
                     PVWKRDDAWQVLLCRRAIEPRRGYWTLPAGFMENDETTTEAAIRETEEEAGARIELGA
                     LFSLINVPHVHQVHLFYLADLLDLGYAAGIESLEVALFREKNIPWLEIAFPTVEYTLR
                     AFFSDLKKIRTGDGGFGLHTDDIRKRIVQVD"
     gene            complement(4796..5395)
                     /locus_tag="VFA14_01020"
     CDS             complement(4796..5395)
                     /locus_tag="VFA14_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HZS80005.1"
                     /translation="MKIIAFPRKEQGTGASRRLRNAGQTPAIVYGGAAAPLNITLDHN
                     ALYHALKKEAFHSSILDLEIDGKVEQVLLRDFQIHAYKQLVLHVDFQRVDAAQKIHVK
                     VPLHFVNAEISPAVKLSSGIISHVMTELDISCLPKDLPEFVAVDLAGLEVGHSIHLAD
                     LQLPNGVTAVSQENLTVATASVPAGKVEAEGAAGDAETK"
     gene            complement(5527..6477)
                     /locus_tag="VFA14_01025"
     CDS             complement(5527..6477)
                     /locus_tag="VFA14_01025"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008110854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HZS80006.1"
                     /translation="MAYENLMVFTGNANPALAQGVVERLGIPLGKANVSRFSDGEVMV
                     EINENVRGKDVFVLQSTCAPTNDNLMEIMLMVDALKRASAGRITAAIPYYGYARQDRR
                     PRSARVAISAKVVANMLQEAGVERVLIMDLHADQIQGFFDIPVDNIYASPVLLGDLVS
                     KDYDDLLVVSPDVGGVVRARALAKRLNCDLAIIDKRRPKANVSEVMNIIGEVENRNCV
                     IMDDMVDTAGTLTKAAEVLKERGARTVVAYCTHPVLSGPALSRIANSPLDELVVTDTI
                     PLSDAVRACSKIRQLTCAGLLAETFRRISKGDSVMSLFAE"
     gene            complement(6619..6695)
                     /locus_tag="VFA14_01030"
     tRNA            complement(6619..6695)
                     /locus_tag="VFA14_01030"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6659..6661),aa:Gln,seq:ttg)
     gene            complement(6775..>7617)
                     /gene="ispE"
                     /locus_tag="VFA14_01035"
     CDS             complement(6775..>7617)
                     /gene="ispE"
                     /locus_tag="VFA14_01035"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HZS80007.1"
                     /translation="PAPAKLNLFLHVTGRRDDGYHLLQTVFQLLDRNDTLDFNIHDNA
                     QIRRINDIPGIAADSDLVVRAARLLQTAGKTRLGADITVHKRLPVGGGLGGGSSDAAT
                     TLLVLNHLWQTGLTRAQLMALGLRLGADVPFFLLGSNAFAEGIGEKLIALKTADRWFV
                     VLEPGISVPTATIFSSPELTRSTKIVRMTDFLEAYDFLGSGFGKNDLEAVAARKFPPV
                     AEAIEWLRTFGNARMTGSGACVFCSFEHEYQADEVLKQLPSRWTGWKARAMQEHPLAG
                     LAAS"
BASE COUNT         1667 a         2100 c         2227 g         1623 t
ORIGIN      
        1 ttgcgtcggc ggtgctggcc atcacgctga actcgggcgc ttacgtgtcg gaaattttcc
       61 gtgccggcat tcagtcaatc gatcgcggcc agagcgaagc ggcgcgctcg ctcggtatga
      121 gctacatgca aacgctgcgc aaggtggtat tgccacaagc gttccgccgc atgctgccgc
      181 cgctgggcaa caatgcgatt gccatcgtca aggattcgtc gctggcctcg gccatcggcc
      241 tggcggaact ggcctatgcg gcacgcacgg tatcgggcgc ttatgcgcgt tactgggaac
      301 cctacctgac tatttcgctg atttactggg tgattacctt attgctgtcg atattcgtca
      361 gacatcttga aaaccgctac ggcaaaggag atgtgcgatg attcacgtca agcaattgca
      421 aaagcagtac ggtgacgcgc atatcctgcg cggcatcgac tgcgacatcg aggagagcga
      481 agtggtgtgt gtaatcggac cgtcgggttc gggcaagagc actttcctgc gctgcctcaa
      541 cggtctggaa gaagcgaccg atggcgagat cgtgatcgat ggcgtcaagg tcaacgatcc
      601 gcgggtcagt ctcgatacgc tgcgcgccgg catgggcatg gtgtttcagc gcttcaacct
      661 gtttccgcac atgccggtgc tggaaaacct gatcatggcg ccaatgcagg tgaagaagct
      721 gtcgcgccgc gaggcggtgc tggtggcgga gaaactgctg cagaaggtcg gcctgctgga
      781 caagatcgac gcctttccaa accagctttc cggtggtcag cagcagcgtg tggcgattgc
      841 ccgcgcgctg gcaatggcgc cgaaggtcat gctcttcgac gaaccgactt ccgcgctcga
      901 tcccgagctg gttggcgaag tgctgacggt gatgaagacg ctggctgaag agggcatgac
      961 catggtcgtg gtcacgcacg agatggggtt tgcgcgcgaa gtatcggatc gcgtgttctt
     1021 catcgatcag ggcgtaatca tggaagaagg tccgccgcag caagtgctgg gcgaaccgcg
     1081 caacgagcgc acgcgcgact tcttgcgcaa ggtgttgtaa gaacctgttc ccgatgagat
     1141 cgggaacagg ctctaatttc tctcatcact atgtgtcgcc ctgaccaaag tcaggggcca
     1201 gtgccgtcag aagtgttgcc gtgtcagcgc aacgcatccg cgcattcctt gaccagcgcc
     1261 gggccgcggt agatcaggcc agaatataac tgcaccagcg atgcgccggc ctgcatcttg
     1321 gccttggcat tgctgccgct gagaatgccg ccgacgccga tgatcggcag cgcatcacca
     1381 agctctgcct tgaggccacg cactacctgg ttcgacaact cgaataccgg cgcgcccgac
     1441 aggccgccgg tttcttcgcc atgcagcatg cctttgacgg cgtcgcgcgt gatggtggtg
     1501 ttggtggcga tgacgccatc gagcttgtgg cgcagcagtg cgtcagcaat gttcttgatt
     1561 tgttcgctat cgatgtctgg agcaatcttc aatgccagcg gcacgtagcg tttgtacctg
     1621 tcggccagac gctgctgcgc ctccttgagt tgagatagca aggcatcgag ctcggaagcg
     1681 ccttgcaact ggcgcaggtt cctggtattg ggcgacgaga tgttgatggt aacgtagccg
     1741 gcgtagggat aaaccttttc caggcaatac aggtaatcag cggcggcgcg ctcgatcggt
     1801 tggtcggcgt tcttgccgat gttcaaacca agcacgcctt gtttgctttg gtaaaaacgt
     1861 gaagcctgta tgttggcgac aaaggcatcg acgccgccat tattgaaacc catgcgattg
     1921 atgatggcat gcgcagccgg caggcggaac atgcgcggct tcggattgcc gggctgggca
     1981 tgcggtgtga ccgttcctat ttcgataaag ccaaagccga gtgcggccag gccatcgatg
     2041 taagagccat ccttgtccag gcccgccgca agaccaaccg aattgggaaa ggtgataccc
     2101 atgaccgtgc gcggatcagc ggcgggttta ctgatgcagc cggtcatgcc gagcgcagcg
     2161 gcgcggcgta aggcagacag agtaaggtgg tgggcggatt ctgcgtcaag ggaaaacagc
     2221 agtggacggg caaaggcgta gagaaattta tcgaacacga tgagtcgctg gaggaaaaag
     2281 tgcggctatt ttaacgtgtc caaggcgctt ttcctgccaa ggcgcagtct attcgtccag
     2341 cgcgatccag ttgctgcccc gcaatgcttc gagcggcttg aagttgatct tgtaggccat
     2401 tttggcgctt tccttgatcc agtagcccag atagacgtag gacatgccga gctgacaggt
     2461 ctgctcgatt tgccacagga tgttgtaggt gccgtaagat gcgctttcgc tatccggatc
     2521 gtagaaggta tagaccgacg acaagccgtc atcgagtacg tcgatgatgc tgaccatacg
     2581 cagcacgccg ctggaatcgc gaaactccac cagacgcgta ttgactctgc tttgcagcag
     2641 gaattgcgtg tattgctcac gactgtcctg atccatgccg ctaccggcgt gtcgcaccga
     2701 ctgatagcgc aggtaaagtt cgtaatgttc gcaggcgtaa gccaggttgg tgaccagcgt
     2761 cacgagctga cggttcctgg cccaggcgcg gcgctggctg cggttgggtg tgaattctgc
     2821 agcattgatg cgtaccggcg tgcaggcgcg gcagccatca caataagggc gataagtaaa
     2881 gatgccgctg cggcggaaac cgctcttgac cagttcagca taaacgtcgg cattgatcag
     2941 atgcgacggc gcggcaactt gtgagcgtgc ctgctggtta tcgagataac tacagggata
     3001 agacgcggtt gcataaaatt gcaacgccga aaacggcagg tctttaccgt gcatcatgcg
     3061 tcttcctgtc gcgcagtcac aagatctgga ggatgccagc aagtaatttg cggcgcgtct
     3121 atcgactgac gcaaatgcgc caggaatgcg ctgcgcggaa tcggcaccgc gcccagcgag
     3181 gccaggtgcg aggtttcctg ctggcaatcg atcatccgca ctccatgctg gcgcaaaaaa
     3241 tgcaccagat aggtcagcgc cactttggaa gcgtcggata cccgcgcgaa catcgattcg
     3301 ccgtaaaaca tccggccgat ggaaacgcca taggcaccgc cgaccagcct gccttccagc
     3361 cacacctccg acgaatgcgc atagccggcc tgatgcaggt ggcagtagct gtcgacgatg
     3421 ccccgggaaa tccaggttcc ggcagtctcc ttgcgcggtt ccgcgcaagc ctgcatgaca
     3481 tcgcggaacg ccgagtcgaa gcgcacattc cagcgcgcat cggtatggcg gctgcgctcg
     3541 accttgcgca aggttttttt caggctgtcg cttacgatga gacgatcggt atacagcacc
     3601 atgcgcggat cggtactcca ccacaggatg ggctggcctt ccgagaacca cggaaaaata
     3661 ccgtggcgat aagcatcgag caaacgctgc ggcgacagat cggcaccgac ggccagcaag
     3721 ccggccggat cggccaaggc cctggaaacg tccgggaagg gcgagtcgac atccagccag
     3781 gaaatcatgc gtgcccccga acgttaatcc acttgtacta tgcgtttgcg aatatcgtca
     3841 gtgtgcagtc cgaaaccgcc gtcaccggtc ctgatttttt tgagatcgga aaaaaaagcg
     3901 cgcagcgtgt attcgaccgt aggaaaggcg atttccagcc aggggatgtt cttttccctg
     3961 aacaacgcta cttccagact ctcgatgccg gcagcgtaac cgaggtcgag caagtcagcc
     4021 agataaaaga ggtggacttg atgcacatgc ggcacattga tcagcgagaa caatgcgccc
     4081 agctcgatac gcgcgccggc ctcttcttcg gtttcgcgga tggctgcttc ggtcgtggtt
     4141 tcatcgtttt ccatgaagcc ggccggtaac gtccagtaac cgcgacgcgg ttcgatagcg
     4201 cgacggcaca gcagcacctg ccatgcatcg tcgcgtttcc agaccggaat cgagccgacc
     4261 accatcttcg gattttgata atgaatggcg ccgcaatggc cgcagacgaa gcgcggacga
     4321 ttgtcatcgg gaggaatccg caaggaaacc ggatgagcgc attcagaaca aaatttcata
     4381 cgaataagaa ctgtcggcga ttttttcaga agcgcccggc ggccagagac acatcatgat
     4441 acatagcaaa gcgcgagtag aagtgtatca ctgtcgtcca tcgtgaaacg cttttatccc
     4501 ccggcatcga gcgattgttc agatgaatca aggcgtggcg gcatgtgtcc ctcgatacga
     4561 cacagtagtg ttcagggact tctccacctg gcgcccacca attattcgat gggcaaggaa
     4621 aggataagag tcaaagacgc aactgactgc gtctttggat tagtctccag gtcaaccatg
     4681 gcaagaaaat cgtcccagca ttggaggacg atgtccggga ggggggccag tcaagcagtg
     4741 cttcgagacc ggaggctgcc ccaccgtggt cagtgatact gaactgaacc agaaattact
     4801 ttgtttcagc gtcacctgct gcgccttcgg cttccacctt gccggccggc accgaagcgg
     4861 tagcaacggt caggttttcc tgtgaaacgg cagtcacacc atttggcagt tgcaggtcgg
     4921 ccaagtggat cgagtgaccg acctccagtc cggccagatc gacggcaacg aattccggca
     4981 gatccttggg caggcaggag atgtccagct ctgtcatgac gtggctgatg atgcccgacg
     5041 acagtttgac tgccggcgag atttcagcgt tgacgaaatg cagaggtact ttgacgtgga
     5101 tcttctgtgc agcgtcgaca cgctggaagt cgacgtgcag aaccagttgc ttgtaagcgt
     5161 gaatttggaa gtcacgcagc agaacctgtt caactttgcc gtcgatttcc agatcgagga
     5221 tcgacgagtg gaaggcttct tttttcagcg cgtgatacag cgcattgtga tccagagtga
     5281 tattcagcgg tgccgcggcg ccgccataaa cgatagctgg tgtttgaccg gcattgcgca
     5341 ggcggcggct cgctccggtc ccctgttctt tgcgtggaaa tgcgatgatt ttcatgttga
     5401 agctccaatg attgcgggac ttgcgtcacg cgttgaacat cccccgcgac caggggattc
     5461 gaagatccct tcatctgcat gaaggaattg aaaccggatg atccaagagc gtattccaaa
     5521 gccgccctac tctgcaaaca gcgacatgac cgagtcaccc ttgctgatgc gcctgaaggt
     5581 ctcggccagc aggccggcac aagtcagctg gcgaatcttg ctgcaggccc ggacggcgtc
     5641 cgatagtgga atcgtgtcgg tgacgaccag ctcgtccagc ggcgaattgg caatgcggct
     5701 cagcgccggg ccggacaaca ctggatgcgt gcaataagcg accactgtcc tggcgccgcg
     5761 ctctttcaat acttccgcag ccttggtcag cgtgccggcg gtgtcgacca tatcatccat
     5821 gatcacgcaa ttgcggtttt cgacttcacc gatgatgttc atgacttctg acacattcgc
     5881 tttggggcgg cgtttgtcga tgatggccag gtcgcagttc aggcgttttg ccagcgcgcg
     5941 ggcgcgcact acgccgccga catcgggcga tacgaccagt agatcgtcat aatccttgct
     6001 caccagatcg cccagcaaaa ccggcgatgc atagatgtta tcgacaggaa tatcaaagaa
     6061 gccctgaatc tggtctgcgt gcagatccat gatcaggacg cgctcgacac ctgcttcctg
     6121 caacatgttg gcaacgactt tggccgaaat cgcaacacgg gccgagcgcg gacgacggtc
     6181 ttggcgggca tagccgtaat aaggaatggc ggccgtgatg cgaccggcag aggcacgctt
     6241 caaggcatca accatgagca tgatttccat aaggttgtcg ttggtcggcg cgcaggtcga
     6301 ttgcagcacg aatacgtctt tgccgcggac gttttcattg atctcgacca tcacttcacc
     6361 atccgagaac ctggaaacat tggccttgcc cagcggaata ccgagccttt cgacgacgcc
     6421 ttgcgccagc gccggattag cgttgccggt aaaaaccatc aggttttcgt atgccatgtg
     6481 ggtattcctg agtactatcg ttcaaaaatc gaaaagccga caatgcgcga cttttttagt
     6541 cttgcgttgg cggcttttat ctgttggttt cttttttctt tagcgggcag acctgtatcc
     6601 aagtctgccc aaattcagtg gcaggggaac aaggattcga accttggaat gctggaatca
     6661 aaatccagtg ccttaaccag cttggcgatt cccctacgca acctgctgct tgccgtcgtt
     6721 ctgcacatct gatcctgtgc aattcgacgt caaaccaatt cctggtgacc ccgatcatga
     6781 agccgccaga ccagcgagag gatgctcctg catcgctctg gctttccagc cggtccatcg
     6841 cgaaggtagc tgcttcaata cctcgtcggc ctggtattca tgttcaaacg aacagaacac
     6901 acatgcgcct gatccggtca tccttgcatt tccgaatgtc ctgagccatt cgatggcctc
     6961 agcgacagga ggaaattttc tggcagccac cgcttccagg tcgttttttc cgaagccgct
     7021 accaagaaaa tcatacgctt ccagaaagtc cgtcattctg acgatttttg tactccttgt
     7081 caattcagga gatgaaaaaa ttgtcgcggt cggaacggat attccgggtt caagtacgac
     7141 gaaccatcga tcagcagttt tcagggcgat caatttttca ccgatgcctt cggcaaaagc
     7201 attacttcca agtagaaaaa acggcacatc ggcacccagt cgcaggccca gcgccatcaa
     7261 ttgtgcgcgc gtcaggccgg tttgccacaa atgattcaac accagcaacg tggtagcagc
     7321 gtctgaggag ccgccaccca gaccaccacc tacaggcagt cgtttatgga cggtgatgtc
     7381 ggcgcccagc cgggtttttc ccgctgtttg cagcagccgg gccgcgcgta ccaccagatc
     7441 gctatcggcg gcaataccgg gaatgtcgtt gatgcgccgg atctgagcat tgtcgtggat
     7501 gttgaagtcc agcgtatcat tgcggtccag caactgaaat acggtttgca gcaggtgata
     7561 accgtcgtcg cggcggccgg tcacgtgtag aaacaggttc agttttgccg gagcggg
//
LOCUS       DASRDH010000040         5227 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1016979,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000040 DASRDH010000000
VERSION     DASRDH010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 5227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5227
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1016979"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="VFA14_01040"
     CDS             complement(<1..523)
                     /locus_tag="VFA14_01040"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006462703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HZS80008.1"
                     /translation="MSQFFQIHPDNPQLRLIRQAAQIIQGGGIVALPTDADYTLVCRL
                     DDKSAMERLRRIHGVDDRHHLTLLCRDLSEIAQYARIDNRQYRLLKAVIPGPYTFILE
                     ASREVPRRLSNPSRKTIGLRVPENPVSHALLAELGQALIGVTLICPDQGDVRTHAEDI
                     HARLKKQIELVIDS"
     gene            complement(598..1473)
                     /gene="htpX"
                     /locus_tag="VFA14_01045"
     CDS             complement(598..1473)
                     /gene="htpX"
                     /locus_tag="VFA14_01045"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016833460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HZS80009.1"
                     /translation="MKRIFLFLATNIAVLVVMSVILSLLGVDRFLTRSGLNLPMLLVF
                     SIVVGFTGSIISLLMSKSMAKWSTGARVIDTPTNATEVWLVNTVNALAQRAGIGMPEV
                     AVYDGEPNAFATGAFRDSALVAVSTGLLRGMTKEEVEAVLGHEVAHIANGDMVTMTLI
                     QGVVNTFVVFLSRVVGYFVDAALRRNSNDSGPGVGYMVTVMVCQIVFGIGASMIVAWF
                     SRHREFRADAGSARLLGGPQPMIDALARLGGIPASGLPESMAAMGINDKPGFMELFST
                     HPPLEQRIAALRGQR"
     gene            complement(1586..2422)
                     /locus_tag="VFA14_01050"
     CDS             complement(1586..2422)
                     /locus_tag="VFA14_01050"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097657 - 3',5'-nucleotide bisphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-nucleoside bisphosphate phosphatase"
                     /protein_id="HZS80010.1"
                     /translation="MLNIDLHCHSNISDGLLPPAEVAARAKANGVDVWALTDHDEIGG
                     VTIAREAAAALGLRYIPGVEISVTWAGQTVHIVGLRIDEKNEALVQGLAALRNGRLQR
                     ARDIAAALETAGIPDAFDGAFKYIGNPDLMSRTHFARYIVEIGRCADIKEVFRNYLCK
                     GKPGYVPHRWATLKDAVDWIRGAGGVAVIAHPGRYQYSELEFRVLFDEFKQYNGAAIE
                     VTTGSHTPDQYEQYARVARHYGFLASMGSDFHGPGESRVDLGALPPLPSGLTPVWHDW
                     GL"
     gene            complement(2546..3571)
                     /locus_tag="VFA14_01055"
     CDS             complement(2546..3571)
                     /locus_tag="VFA14_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HZS80011.1"
                     /translation="MQNRMVTLALQGGGSHGAFTWGVLDRLLEDGRMSFEGISGASAG
                     AMNAVVLAHGFHIGGREGARAALSAFWKRIAIKDPFSFLSPEVTPGGANLLTHTTPAA
                     MQAFLAMTRFFSPTQLNPLDINPLRDILEEQVDFARLSKSNDIKLFIAATKVSTGTLK
                     IFRNRELTLDALLASACLPSIHRPIEINGEAYWDGGLTANPPIFPLLHLCSSHDLLVV
                     LLHPCNRPGTPTSINDINQRLFEISFSSAFFTEVGGLALVKREAERGMLAFGTLERRL
                     KRLNMHLVEASELMSQLDALSKLNTKHSFIHGLYDAGRQAAEHWMEQHFHTLGRRSSF
                     DLGRYLQ"
     gene            complement(3645..4886)
                     /locus_tag="VFA14_01060"
     CDS             complement(3645..4886)
                     /locus_tag="VFA14_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase family protein"
                     /protein_id="HZS80012.1"
                     /translation="MNDRKNYGFTTTILHSDRRKAIEHGSLHKPIHTSVAFGYQDARQ
                     LAAVFQGKQSGYRYGRQGNPTVSALEDKVTRMEGGLSTICFASGMAAIGAVVQGLLRE
                     GDHVVSSAFLFGNTNSMWTTVNAQGARVTMVDATDVELVAAALTPQTRVVFVETIANP
                     RTQIADLQGIGDLCRARGILYIVDNTMTSPYLFQPKAVSAGLVINSLTKSIGGHGNAL
                     GGALTDTGLYDWSRYPHIADSYRKNPQAYWGMAQIRAKGLRDFGASLGPEAAHHIAVG
                     AETMALRQERQCASALALAHMLDADARVAAVYYPGLPSHPQHVLATALFRSYGSLLSF
                     ELRNGIDCFDYLNRLQLAVASSNLGDTRTLVIPVAHTIFHEMGQERRAAMGIAESLIR
                     VSIGLEDTDDLVEDFRQALDS"
     gene            4895..>5227
                     /locus_tag="VFA14_01065"
     CDS             4895..>5227
                     /locus_tag="VFA14_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS80013.1"
                     /translation="MRKAAKRKKTVMISQFGWHRAQSRAGTSKFGSWLHAEQIEQILW
                     LPGGSFAFARQAPFARGKNEATKEHPEYFLILGMDDCRADSTRDTISLFERPLASGMS
                     HVSIHMNWT"
BASE COUNT         1212 a         1499 c         1473 g         1043 t
ORIGIN      
        1 cgctgtcgat gaccagttcg atctgttttt tcagacgtgc gtggatatcc tcggcatggg
       61 tccgcacatc gccctgatcc ggacagatca gcgtgacgcc gatcaacgcc tgccccagct
      121 ctgccagcaa ggcatgggag accggatttt ccggcacgcg cagaccgatg gtcttgcgcg
      181 acggattgct caggcgacgc ggcacttccc tgcttgcttc caggataaag gtatacggcc
      241 ccggtataac ggctttgagc aaacgatatt gacggttatc aatcctggcg tattgtgcga
      301 tttcgctaag gtcgcggcac agcagcgtca aatggtgtct gtcatcgaca ccatgaatgc
      361 ggcgcagacg ctccatcgcg ctcttgtcat cgagccggca caccagtgtg taatcggcat
      421 cggtcggcaa agcgacaatg ccgccgcctt gaatgatttg cgcagcctgc ctgatcaagc
      481 gcagttgcgg attatcgggg tgaatctgaa aaaattgact catgcgcgag gtttcaaaaa
      541 agaaaaaacg gcggtggcga tatcaccccg ccgttttttc acctgattac accggcatta
      601 ccgctggccg cgcaaggcgg cgatacgctg ctccagaggc ggatgcgtgg aaaacaattc
      661 catgaatccc ggcttgtcgt tgatgcccat agccgccatc gattccggca gaccgctcgc
      721 cggaatgccg cccagccgtg ccagtgcgtc gatcatcggc tgcggaccgc cgagcaatct
      781 ggcggaaccg gcatcggcgc gaaactcacg atgacgcgag aaccaggcca cgatcatcga
      841 ggcgccgatg ccaaacacga tctggcacac catcaccgtc accatgtagc cgacgccggg
      901 accggaatca ttgctgttgc ggcgcagcgc cgcatcgacg aagtaaccga ccacgcgcga
      961 caggaacacg acgaaggtat tcactacacc ctgtatcagc gtcatggtca ccatgtcgcc
     1021 attggcgatg tgggcgactt cgtggccgag tacggcttcg acttcttcct tggtcatgcc
     1081 tcgcagcaac ccggtcgaca ccgcaaccag cgccgagtcc ctgaaagcac cggtggcgaa
     1141 ggcattcggt tcgccgtcat agacggcgac ttcgggcata ccaatgccgg cgcgctgcgc
     1201 cagggcgttc acagtattga ccaaccagac ttcggtggca ttggtcggcg tgtcgatcac
     1261 gcgcgcgccg gtactccact tcgccatgga tttgctcatc agcaacgaaa tgatcgagcc
     1321 ggtaaaacca accacgatgg aaaacaccag cagcatcggt agattcaatc ctgagcgggt
     1381 caggaatcta tccacaccga gcaaagacaa aatcacgctc atcaccacca gtacggcgat
     1441 attggtggcg aggaaaagga atatgcgttt cattcgggaa aactcctatc gcaaaagtgg
     1501 ttattagata ggactggcag aaaaaaattc aagagataac gacaatttca ttgagaatct
     1561 ggaaagggat ggttcttaca tccggtcaca ggccccaatc gtgccacacc ggcgtcaatc
     1621 cggatggtag cggcggcaag gcgcccagat cgacacgtga ctctcccgga ccatggaagt
     1681 ccgaacccat cgatgccagg aaaccgtaat gtctggcaac acgagcatat tgctcatact
     1741 ggtcgggggt atggctgccg gtagtgactt cgatggctgc gccgttatat tgtttgaatt
     1801 catcgaataa aacgcggaac tcgagttcac tgtactgata gcgtcccgga tgcgcgatca
     1861 ccgccacgcc gccggcgcca cgaatccagt cgaccgcgtc cttgagcgtg gcccagcgat
     1921 gcggcacata accgggtttg cctttgcaca gataattgcg aaacacttcc ttgatgtcgg
     1981 cgcaacggcc gatttcgacg atatagcgag cgaaatgcgt gcgtgacatg agatcgggat
     2041 taccgatgta tttgaacgca ccatcaaaag catcgggaat gccggccgtc tccagcgcag
     2101 ccgcgatgtc gcgagcgcgc tggagacggc cattgcgcaa agctgccaga ccttgcacca
     2161 gcgcttcatt cttttcgtcg atgcgcaagc cgacgatatg cacggtctgc ccggcccagg
     2221 taaccgaaat ttcaacgccg gggatgtaac gcagaccgag cgctgcggca gcctcacgcg
     2281 ctatggtgac accaccgatt tcgtcatggt cggtcagtgc ccagacatcg acgccattgg
     2341 ctttggcgcg tgcggccact tccgccggcg gcagcaggcc gtcggagata ttggaatggc
     2401 agtgcaaatc gatgttgagc atgatgaagc gcgcctgaaa aacaacgatg aatggctata
     2461 ttgtacgccg catggagctg ttgataatgc caaccgcaga tggcaaggaa tgttgggcat
     2521 aaagggtttc atgacgcaat gcctgctatt gcaggtaacg tccgaggtcg aaggaagaac
     2581 gccgtcccag tgtgtgaaaa tgctgctcca tccagtgctc ggcagcctgc cggcctgcat
     2641 cgtaaaggcc gtggatgaat gaatgcttgg tgttgagttt actcagggca tcgagttggc
     2701 tcatcagctc actggcttcg acaaggtgca tattgagccg cttcaggcgc cgctccagcg
     2761 tgccgaaggc gagcatgccg cgctccgcct cgcgcttgac cagagccagc ccacccactt
     2821 cggtaaagaa ggccgaactg aaactgatct cgaagagtcg ctgattgata tcattgatgg
     2881 aagtcggcgt gccggggcga ttgcacggat gtaatagcac taccagcaga tcgtgcgatg
     2941 agcacaaatg cagcaaggga aaaatcggtg gattggccgt cagtccgcca tcccaatacg
     3001 cctcgccatt gatctcgatc ggtctatgta tcgagggtag acaggccgaa gccagcaagg
     3061 catccagcgt cagttcgcga ttgcggaaga ttttcagcgt accggtgctg accttggtgg
     3121 cagcgataaa caatttgatg tcattgctct tgctcaagcg cgcgaagtcg acctgctcct
     3181 cgagaatgtc acgcaaagga ttgatgtcga gcggattcag ctgcgtcggc gaaaaaaagc
     3241 gcgtcatcgc cagaaaagcc tgcatcgctg ccggcgtggt gtgcgtcaat aggttggctc
     3301 cgcccggtgt cacctccggt gacaaaaaac tgaacggatc cttgatagca atgcgtttcc
     3361 agaatgcgga gagcgccgcg cgcgcacctt cgcgtccacc gatatggaag ccatgcgcca
     3421 gtacgaccgc gttcatggcg ccagcactgg cgccgctgat accctcaaat gacatgcgac
     3481 cgtcttccag caaacggtcc agcacacccc aggtaaaagc gccgtgcgaa ccgccacctt
     3541 gcaacgccag tgtcaccatc ctgttttgca tgctgctcct tcagtgagta ttactatttg
     3601 ttcggctcat catgatgaaa agtcatgaca ggaagagcaa gcgatcagga atccaacgcc
     3661 tgccggaaat cttccaccag atcatccgta tcttccagac cgatcgatac ccgaatcaga
     3721 gattcggcga tgcccattgc agcacggcgt tcctgtccca tttcatggaa aatcgtgtgc
     3781 gccaccggaa tcaccagggt acgcgtatca cccagattgc ttgacgccac cgccagttgc
     3841 aaacggttca ggtaatcgaa acagtcgata ccattcctga gttcgaaact cagcaggcta
     3901 ccgtaagaac ggaacaaggc agtcgccagc acgtgctgcg gatgcgaagg cagaccggga
     3961 tagtacacgg ctgccacgcg cgcatcggca tccagcatat gcgccaacgc caacgcactg
     4021 gcgcattggc gttcctgacg caaggccatg gtttccgcac cgacggcgat atgatgcgcg
     4081 gcctccggtc ccagcgaagc gccgaaatca cgcaaccctt tggcgcgaat ctgcgccatg
     4141 ccccagtacg cttgcggatt tttcctgtag ctgtcggcaa tgtgcggata acgcgaccag
     4201 tcgtacaaac cggtgtcggt cagtgcgccg ccgagcgcat taccatgacc gccgatggat
     4261 ttggtcagcg aattgatcac caacccggcg ctcaccgcct tgggctggaa caggtaaggc
     4321 gaggtcatcg tgttgtcgac gatgtacagg atgccacgcg cacggcagag atcgccgata
     4381 ccttgcaaat cagcgatctg ggtacgtgga ttggcgatgg tttcaacgaa aaccacacgt
     4441 gtctgcggcg tcaatgcggc tgccaccagt tcgacgtctg tggcatccac catggtcacg
     4501 cgcgcgccct gcgcattgac cgtcgtccac atgctgttgg tattaccgaa caggaaagcc
     4561 gacgatacca catgatcgcc ttcacgcagc aagccctgga cgacggcgcc gatggccgcc
     4621 atgccagagg cgaaacagat ggtcgacaga ccgccctcca tcctggtgac cttgtcttcc
     4681 agcgccgaaa ccgtcggatt gccctggcga ccatagcgat agccggattg tttgccctgg
     4741 aatactgcgg ccagttgacg cgcatcctga tagccgaaag ccacggaagt gtgaatcggt
     4801 ttgtgcagcg agccatgctc gatcgcttta cggcggtcac tgtgcaaaat ggtggtggtg
     4861 aaaccgtagt tcttcctgtc gttcatggta ttgaatgcgc aaggcggcaa aacgtaagaa
     4921 aacagtgatg ataagccagt ttggctggca ccgcgcccag tccagggcgg gaacatcaaa
     4981 attcggttct tggctgcatg ccgagcaaat cgaacagata ctctggctgc caggcggctc
     5041 gtttgcattt gcgcggcaag ctccgtttgc tcgcggcaaa aatgaagcaa cgaaggagca
     5101 tccggagtat tttctgattc tcgggatgga tgattgtcgc gcggattcca ccagagacac
     5161 gatttcgctt ttcgaaagac ctttggcttc tggaatgagc cacgtgagca tacacatgaa
     5221 ttggact
//
LOCUS       DASRDH010000041         4265 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1696653,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000041 DASRDH010000000
VERSION     DASRDH010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 4265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4265
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1696653"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1009)
                     /gene="dxs"
                     /locus_tag="VFA14_01070"
     CDS             complement(<1..1009)
                     /gene="dxs"
                     /locus_tag="VFA14_01070"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007877886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HZS80014.1"
                     /translation="MLDTINSPADLRQLPRTQLKLLAGELRAFLLDSVAKTGGHLSSN
                     LGTVELTIALHYVFNTPEDRIVWDVGHQTYPHKILTGRRDQMSTLRQRNGISGFPRRV
                     ESEYDTFGTAHSSTSISAALGMALAARTKGEERHAIAVIGDGAMTAGMAFEALNNAGI
                     CEDVNLLVILNDNDMSISPPVGALNRYLARLMSGQFYAKARSVGKSMLPAPVLELAKR
                     FEEHAKGMIVPATMFEEFGFNYIGPIDGHDLDSLIPTLQNLKNLKGPQFLHVVTRKGQ
                     GYKLAEADPVLYHGPGKFDPDEGVKPAAASKLTYMQVFGEWLCDMAQQDKTLIGITPA
                     MR"
     gene            complement(1054..1944)
                     /locus_tag="VFA14_01075"
     CDS             complement(1054..1944)
                     /locus_tag="VFA14_01075"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HZS80015.1"
                     /translation="MSFVDWMQYIQEGMEATLDTYLPAVTAMPAHRLHEAMRYTALGG
                     GKRVRPLLLYAAGELFDASGQLLARAAAAVEMIHVYSLVHDDMPCMDDDALRRGKPTV
                     HIRYDEPTALLVGDALQAQAFAVLSVPLDDQQAARQLKMLNLLALAAGSTCMCGGQAI
                     DLASVGMSLSLAELEQMHRLKTGALLHASILLGAYSGKLLSAPEAAALDAYASAIGLA
                     FQVVDDILDATADSLTLGKTAGKDAAGNKPTYVSILGLDASQALAEKLRHDARQALAQ
                     FGDKARRLHELADLIVQRKA"
     gene            complement(1965..2216)
                     /locus_tag="VFA14_01080"
     CDS             complement(1965..2216)
                     /locus_tag="VFA14_01080"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HZS80016.1"
                     /translation="MPKNPAKVTQPASFDAAMQELERLVVQMEAGELPLEALVAAYRR
                     GAELVKFCAVQLDRIDEQVKVLEGDMLKPLVTDVTETRE"
     gene            2435..3553
                     /locus_tag="VFA14_01085"
     CDS             2435..3553
                     /locus_tag="VFA14_01085"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HZS80017.1"
                     /translation="MSDLAIFAKLARANTQLPVDVYFDETFFRHEIHQLFQQGPRYAG
                     HELMVPDIGDYASLPWENEGRILVRNAQGIELISNVCRHRQAKMLSGRGNTRHIVCPL
                     HRWTYDLKGELIGAPHFSETPCLNLSRTPLQRWNGLLFENDDKGCINVATQLRQLGVD
                     KELDFSGYLFDHVEIHTCDYNWKTFIEVYLEDYHVDPFHPGLGSFVSCDDLTWEFGKN
                     YSVQTVGINHGLTRSGSPAYRKWQEQVLRLDDGQLPKYGAIWLTLYPNIMVEWYPYVL
                     VVSTLWPSGPQKTTNVVEFYYPEEIALFEREMVEAERAAYMETCAEDDEIALRMDAGR
                     RILMQRGTSEVGPYQSPMEDGMQHFHEWYRSQIAVQEN"
     gene            complement(3711..>4265)
                     /locus_tag="VFA14_01090"
     CDS             complement(3711..>4265)
                     /locus_tag="VFA14_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007878253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HZS80018.1"
                     /translation="QNLLRDVALIAVILVSLWCTPKTAHAGNDFTWGPIIEVAKLFAG
                     IFVTIIPVIAMLRAGEAGAFGAVVRTVTGADGQPIDAMYFWITGVLSSFLDNAPTYLV
                     FFHTASGDAHTLMTTLGSTLAAISAGAVFMGANSYIGNAPNMMIKAIAEGRGIRMPSF
                     FGYMLWSLTILMPLFVVMTFLFFV"
BASE COUNT          959 a         1228 c         1197 g          881 t
ORIGIN      
        1 cacgcatggc aggcgtaata ccgatcaggg tcttgtcctg ctgcgccatg tcgcatagcc
       61 attcgccgaa cacctgcata taggtcagct tgctggcggc ggccggcttg acgccttcgt
      121 ccggatcaaa tttgccggga ccgtgataga gtaccggatc agcttcggcc agtttgtagc
      181 cttggccttt tctggtaacc acgtgcagga attgcggtcc cttgaggttc ttcaggtttt
      241 gcagcgtcgg gatcaaggaa tcgaggtcgt gtccatcgat cggtccaatg tagttgaaac
      301 cgaattcctc aaacatggtg gccggcacga tcatgccctt ggcgtgttct tcgaaacgtt
      361 tggccagttc cagcaccggc gccggcagca tggacttgcc gacgctcctg gccttggcgt
      421 agaactgccc ggacatcaag cgcgccagat aacgattgag cgcaccgacc ggcggcgaaa
      481 tcgacatatc attgtcgttg aggatcacca gcagattgac atcttcacag atgccagcgt
      541 tgttcaaggc ctcgaaggcc atgccggcag tcatcgcgcc gtcgccgatg acggcaatcg
      601 cgtgacgctc ctcgcctttg gtcctggcgg ccagagccat acccagggcg gcggaaatcg
      661 aggtcgagga atgggcggtg ccgaaggtat cgtattcgct ctcgacgcgg cgcggaaaac
      721 cggaaatgcc gttacgctgg cgcagcgtcg acatctggtc gcgacgacca gtcaggatct
      781 tgtgcggata ggtctgatgg ccgacgtccc agacgatgcg gtcttccggt gtgttgaaca
      841 catagtgcaa ggcaatcgtc aactcgaccg taccaagatt ggaagaaaga tgcccgccgg
      901 tcttcgctac cgaatcgagc aggaaggcgc gcagttcacc agccaatagc tttaactggg
      961 tacgcgggag ttgccgcaaa tcggctggac tgtttatcgt atcaagcatg ccaggatagt
     1021 cttgtgttaa tttgctgcgc tgccaacacg ctcttaggct tttcgttgca cgatcagatc
     1081 ggcaagctca tgcaggcgcc gcgccttgtc accgaattgc gccaatgcct gacgcgcatc
     1141 atgacgcagt ttttccgcca gcgcctgcga tgcgtcaagg ccgaggatcg atacataggt
     1201 tggcttgttg ccggcagcat ccttgccggc ggtcttgccg agcgtcaggg aatccgccgt
     1261 tgcgtcgaga atatcatcaa ccacctggaa cgccagacca atggcgcttg cgtaggcgtc
     1321 cagcgccgcc gcttccggcg ccgacagcag cttgccgctg taagcgccga gaagaatgga
     1381 ggcatgcagc aacgcgcctg tcttgagccg gtgcatctgt tccagttccg ccagcgacag
     1441 gctcatgccg acgctggcca gatcgatggc ctggccgccg cacatgcagg tcgagccagc
     1501 ggccagcgcc agcaggttca gcatcttcaa ctggcgcgca gcctgctgat cgtccagagg
     1561 caccgacagg acggcaaacg cctgcgcctg caaggcatcg cccaccagca aggcagtcgg
     1621 ctcatcatac cggatatgta cggtcggctt gccgcgccgc agagcatcat catccatgca
     1681 cggcatgtcg tcgtgtacca gcgagtagac atggatcatt tccactgcgg cggcagcccg
     1741 cgccaacagt tgtccagaag catcgaacag ctcgcctgca gcatacagca gcaagggacg
     1801 cacacgcttg ccgccgccta gggcagtgta gcgcatggcc tcatgcaaac gatgcgccgg
     1861 cattgccgtg accgccggca aataagtgtc gagcgtcgct tccatgcctt cctggatata
     1921 ttgcatccag tcaacaaaag acatggtggc cgctatccct gtattcattc gcgcgtctcc
     1981 gtaacatcgg tgaccagcgg cttgagcata tcgccttcca gcaccttgac ctgctcatct
     2041 atcctgtcca gttgcaccgc gcagaatttg accagctccg cgccccgcct gtatgccgcg
     2101 accagggctt ccagcggcag ctcgccggct tccatctgga cgaccagtcg ctccagctcc
     2161 tgcatggcag catcgaacga ggcaggctga gtcactttgg caggattctt tggcatggca
     2221 gtcattttag atacgatccg tcattttagt gcaaaccgct agacaggcgc gtagacgtag
     2281 aatgtgttga cgatctcatc agcttcccga taaggcgaac cagggttttg tggcataatc
     2341 gcggtttcat ccaaaatttc cttttcatat ttgaacacag gtttttgcgg attctttctc
     2401 tcttaggttg gtgcaaatta atttgggggc ggagatgtcc gatcttgcta tttttgccaa
     2461 actggcgcgc gctaacacgc aactaccggt cgatgtctat tttgacgaga catttttcag
     2521 gcacgaaatt caccagttgt ttcagcaagg cccgcgctat gcaggccatg agctgatggt
     2581 tcccgatatc ggcgattatg caagcttgcc atgggaaaac gaaggtcgca tactggtacg
     2641 caatgcgcag ggtatcgagc tgatttccaa cgtctgccgt catcgtcaag ccaaaatgct
     2701 gagcggtcgc ggtaacacgc gccatatcgt ctgcccgctg catcgctgga cttatgatct
     2761 caagggtgag ttgatcggcg caccacattt cagcgaaacg ccttgcctga acctgtccag
     2821 gacaccgctg cagcgatgga acggtctact gttcgagaac gatgacaagg gctgcatcaa
     2881 cgtcgctacc caattgcgcc agctgggcgt tgacaaagag ctggattttt ccggttatct
     2941 gttcgatcac gtcgaaatcc atacctgcga ttacaattgg aagacgttca tcgaagtcta
     3001 cctggaagac taccatgtcg acccatttca tcccggtctg ggcagcttcg tcagctgcga
     3061 cgatctgaca tgggagttcg gcaagaacta cagcgtgcaa accgtgggca tcaatcacgg
     3121 cctgaccagg tccggttcgc cagcttacag gaaatggcag gagcaagtgc tgcgcctgga
     3181 cgacggccag ttgccgaaat acggcgcgat ctggctgacg ctctatccca atatcatggt
     3241 cgagtggtat ccgtatgtgc tggtagtgtc gacgctgtgg cccagcggtc cgcaaaaaac
     3301 caccaatgtg gttgagttct actacccgga agaaatcgcc ctgttcgaac gcgagatggt
     3361 ggaagccgag cgtgctgcct acatggaaac ctgcgccgaa gatgacgaga tcgccctgcg
     3421 catggatgcc ggccgacgta tcctgatgca acgcggcacc agcgaagtcg gcccgtacca
     3481 atcgccgatg gaagacggca tgcaacattt ccacgaatgg taccgctcgc aaatcgccgt
     3541 acaggaaaat tgacgctcga agtggatggg acgcccctga tcgatgcgca tcgatcaccc
     3601 gtttaaagcc actaacacaa cactgatgac aaagcgcgcc gacggagaca gtacgaaggt
     3661 acggcaggca aggaggcgta acgtaatcag cggcgttgtg ttaacggctt ttaaacaaaa
     3721 aacaggaaag tcatcaccac aaacaatggc atcaggatag tcagcgacca tagcatatag
     3781 ccaaaaaaag atggcatcct gatgccacgg ccttctgcaa tcgccttgat catcatgttg
     3841 ggagcattgc cgatatagct gttggctccc atgaatacgg cgccggccga aatcgccgcc
     3901 aacgtggaac cgagcgtcgt catcagcgta tgcgcatcgc ccgaagcggt atgaaagaac
     3961 accaggtagg tcggcgcgtt gtcgaggaac gacgacagca cgccggtgat ccagaaatac
     4021 atggcatcga tgggctgtcc atcagcgcca gtgacagtgc gcaccaccgc gccgaaagcg
     4081 cctgcttcgc cggcgcgcag catggcgatg acgggaatga tggtcacaaa aataccggcg
     4141 aacaatttcg ctacttcgat gatcggcccc caggtaaaat cattgcctgc atgcgctgtt
     4201 ttcggcgtac accacaagga aaccagaatg accgcgatca gagcgacatc gcgcaacaag
     4261 ttctg
//
LOCUS       DASRDH010000042         2516 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_225772,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000042 DASRDH010000000
VERSION     DASRDH010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2516
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225772"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..777)
                     /locus_tag="VFA14_01095"
     CDS             complement(<1..777)
                     /locus_tag="VFA14_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013234078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HZS80019.1"
                     /translation="MQYVSTRGDSATSFFSEILLSGLAPDGGLYLPVSYPRVSGAELD
                     RWRALSYADLAYEVLKKFVTDLPDADLKALVRETYTADVYCNTRAGEDAAQITPLRTL
                     EDRNGRKLMLQALSNGPTLAFKDMAMQLLGNLFEYALARNGAELNIVGATSGDTGSAA
                     EYAMRGKKGIRVFMLSPYKKMSAFQTAQMFSLQDPNIFNLAVQGVFDDCQDIVKAISS
                     DPGYKAAQKIGTVNSINWARVVAQVVYYFRGYLVATANNDQ"
     gene            complement(838..1530)
                     /locus_tag="VFA14_01100"
     CDS             complement(838..1530)
                     /locus_tag="VFA14_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006461348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HZS80020.1"
                     /translation="MRLFRHTLAAISLGLLVVSAGASPTNPQNGTDYRTLEQIQPTES
                     GDKIEVTEFFWYNCSHCAVLEPVLSAWVKKQGDRIVFRQVPIAFRDSLIPQQKLYYAL
                     EAMGKTAEMNPKIFQAIHVENQRIDTDKTILAFIEKQGIDKQKFLDMYNSFGVQARVR
                     RAAQLQEAYKIDGVPVLAIGGRFLTSPAMLGASMVNQPESVLNAASLQVIDHLIARLE
                     AEKGSTGAGRKR"
     gene            complement(1561..2199)
                     /locus_tag="VFA14_01105"
     CDS             complement(1561..2199)
                     /locus_tag="VFA14_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HZS80021.1"
                     /translation="MNTQVKRYHPNLCRQAGGTFLGLILGLIVGLGIAVVVALAITRS
                     PVPLGNKLVRQERSELSPSQTSDPNKPLYGKDVVREAPSAPSAAQDADWNESAPDIEE
                     PDMPPAKADVPQDKPDVVTKDQLARSGSTAADRWIYFLQTGAFRQRADAENARAQLAL
                     LGFEAKVSERPSSNGILYRVRIGPVTQSEIVNRIRSKLSEDGINASIVRIPK"
     gene            complement(2208..>2516)
                     /locus_tag="VFA14_01110"
     CDS             complement(2208..>2516)
                     /locus_tag="VFA14_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DALR anticodon-binding domain-containing
                     protein"
                     /protein_id="HZS80022.1"
                     /translation="DEATLDQVTDLSPLTSPREIALLARLADYPETLVRALQELGPHQ
                     LAFYLRDLASELHGYYNAERVLVDDVTTRMARLALISATRQVLRNGLALVGVSAPSRM
                     "
BASE COUNT          540 a          720 c          701 g          555 t
ORIGIN      
        1 ctggtcatta ttggccgtgg caacgagata gccgcggaag taatacacca cctgcgccac
       61 aacgcgcgcc cagttgatcg aattgacggt gccaatcttt tgcgcggcct tgtagccggg
      121 atcgctggag atagccttga cgatgtcttg gcaatcatcg aatacgccct ggaccgccag
      181 attgaaaata ttgggatcct gcaggctgaa catctgcgcc gtctgaaacg cgctcatctt
      241 tttgtacggc gacagcatga agacgcggat accttttttg ccgcgcatgg cgtattcggc
      301 agcgctgcca gtgtccccgg aagtcgcacc gacgatgttc agttccgcgc cgttcctagc
      361 caaagcatac tcgaacaaat tgcccagcaa ctgcatcgcc atgtccttga aagccagcgt
      421 cgggccgttg gacaaggctt gcagcatcag cttcctgcca ttcctgtctt ccagcgtgcg
      481 cagcggcgtg atctgcgctg catcttcgcc ggcgcgcgta ttgcaataca catcggcggt
      541 ataggtctcg cgcaccagcg ccttcaggtc agcgtcgggc aggtcagtga cgaatttttt
      601 cagcacttcg taagctagat cagcatacga caacgcgcgc caccgatcca gttcggcccc
      661 gctcacgcgc ggataactga ccggcaggta aagaccgcca tccggcgcca gaccgctcag
      721 caggatttcg gaaaagaaag atgtcgcaga atcaccgcgc gtcgaaacat attgcataac
      781 gtaggcaaat cttgaaatag tagcccagga agaacaggaa ttctcaatag caggggttta
      841 cctcttcctg cctgctccgg tgctaccttt ctccgcctcc agcctggcga tcagatgatc
      901 aatcacttgc agcgaggcgg cattcagcac cgactccggc tggttgacca tggaagcgcc
      961 caacatcgca ggcgacgtca ggaaccggcc accgatggcg agaacaggca cgccatcgat
     1021 tttataagct tcctgcaact gtgctgcgcg gcggaccctg gcctggacgc cgaaagaatt
     1081 gtacatgtcc agaaacttct gcttgtcgat accttgcttc tcaatgaagg ccaagatggt
     1141 cttgtcggta tcgatgcgct ggttttcaac gtggatggcc tgaaaaatct tcgggttcat
     1201 ttccgccgtc ttacccatgg cttccagcgc ataatataat ttttgctgtg gaatgagcga
     1261 gtcgcgaaaa gcgatcggca cctgccgaaa aactatcctg tcaccctgct ttttgaccca
     1321 ggcgctcagt accggttcca gcacggcgca gtgcgaacag ttgtaccaga agaattcggt
     1381 gacttcgatc ttgtcgccgg attcggtcgg ctggatttgc tccagtgtgc ggtaatcggt
     1441 gccgttttgc ggattggtgg gagacgcgcc agccgaaacg actagcaaac ccaggctgat
     1501 ggcagccagg gtatgacgga acaaacgcat gaacttgatc ccttctcaga caggttgaaa
     1561 ttattttggt atgcgcacaa tggaagcatt gatgccgtct tcagacagct tgctgcgtat
     1621 ccggttcact atttcagatt gtgtgactgg gccgatgcgc acgcggtaca ggataccgtt
     1681 actggagggc cgctcgctga ctttggcttc gaatcccagt aacgccagct gcgcgcgcgc
     1741 attctcggcg tcggcccgtt gacggaaagc gccggtttgc aggaaataga tccatctgtc
     1801 agcggcagtg ctgccggatc tggcgagctg atctttagtg acgacgtctg gtttgtcctg
     1861 cggcacatcg gcttttgccg gcggcatgtc gggttcctcg atgtcgggtg ccgattcatt
     1921 ccagtctgca tcctgagctg cggatggcgc tgacggcgct tccctgacga cgtccttgcc
     1981 gtacagcggc ttgttggggt cggaagtctg gctcggcgac agttcgctac gctcctgcct
     2041 gacaagcttg ttgccgagcg ggacaggcga cctggtgatc gccagcgcca ccacgacggc
     2101 gatgccgaga ccgacgataa ggccaagaat caaaccgaga aaagtaccgc cggcttgcct
     2161 gcaaagattc ggatgatatc gtttcacttg tgtattcacc tgcaggatta cattcttgat
     2221 ggtgcggata cgccgaccag cgcgagacca ttgcgcagca cctgacgtgt cgcgctgatc
     2281 agtgccagac gcgccatcct agtagtaaca tcatcgacca acacacgttc agcattgtag
     2341 taaccatgca attcacttgc tagatcacgc aggtagaaag caagctgatg cggccccagt
     2401 tcctgcaagg cgcgcaccag cgtttccgga tagtcggcca gcctagccag cagagcgatt
     2461 tcacgcggcg atgtcaacgg cgacagatca gtgacctgat cgagcgtcgc ttcgtc
//
LOCUS       DASRDH010000043         2153 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1351352,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000043 DASRDH010000000
VERSION     DASRDH010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1351352"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..933
                     /locus_tag="VFA14_01115"
     CDS             <1..933
                     /locus_tag="VFA14_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007879155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HZS80023.1"
                     /translation="SAGTLYVARWFQTSGVGPGAGTLKWIRLDHATSAQIKALADQFK
                     ASDIMDIKTSDPQDMSYTKIPFDGKTNWIRLHPGMEQAAVFLETHRYAALMGGSLGFT
                     KMEGTTVNAKDKIAYLAMSYVQTSMLDGSGGIEVQGPKAGAIYALNMQDGQRDLSGAV
                     IDSAWVPVDMVPPAALIGEDLKQPDALGNLANAERIANPDNIKFSEKLRTLFIGEDSD
                     MHVNNFLWAYHIDSGRLSRILSCPAGAESTGLHAVDEINGWTYIMSNFQHAGAWEKPL
                     HDKVRERLDPLVRANYRDRFGAMVAYLTGVQLPA"
     gene            1057..1890
                     /gene="hpnD"
                     /locus_tag="VFA14_01120"
     CDS             1057..1890
                     /gene="hpnD"
                     /locus_tag="VFA14_01120"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008329435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HZS80024.1"
                     /translation="MSPDEYCQQKAVRSGTSFYYSFLFLPPERRRAITALYAFCREVD
                     DIVDDTEDVSVARAGLMWWRKEIAAMFDGIPSHPVTQALRPHVAAYALKGEHLHAIID
                     GMEMDLEQTRYLDFPGLQRYCWHVAGVVGILSASIFGVTQDGTLRFAEKLGLAFQLTN
                     IIRDVGEDGRKGRIYLPVDELQRFSVSAADILAARHSENFEKLMRFQAERAQRLYDEA
                     FALLPRQDRRAQRPGLMMAAIYRTLLDEIGHDGFHVLNQRIALTPIRKLWLAWKTHLR
                     G"
     gene            1883..>2153
                     /locus_tag="VFA14_01125"
     CDS             1883..>2153
                     /locus_tag="VFA14_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007877622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HZS80025.1"
                     /translation="MAENLDHIAVIGGGWAACAAAVTLAQAGRRVSLFESARILGGRA
                     RRVESDGHALDNGQHILLGAYCATLKIMKAVGIDADRMLLRLPLQL"
BASE COUNT          420 a          667 c          648 g          418 t
ORIGIN      
        1 tcggctggca cgctgtacgt cgccaggtgg ttccagactt cgggcgtcgg tccgggcgcc
       61 ggcacactga agtggatcag actggaccat gcgaccagtg cgcagatcaa ggcgctggcc
      121 gaccagttca aggcgtccga catcatggac atcaagacca gcgatccgca ggacatgtcc
      181 tacacgaaga ttcccttcga cggcaaaacc aactggatca ggctccatcc cggcatggag
      241 caggccgccg ttttcctcga aacccaccgc tacgccgcgc tcatgggcgg cagtctcggt
      301 ttcaccaaga tggaaggcac gaccgtcaac gccaaagaca agatcgccta tttagccatg
      361 tcctatgtgc agacttcgat gctggatggc tccggcggca tcgaggtgca aggtccgaag
      421 gccggcgcca tctacgcgct gaacatgcag gacggccagc gcgacctgtc cggcgctgtc
      481 atcgacagcg cctgggtgcc ggttgacatg gtgccgccgg cggccctgat cggcgaggat
      541 ctgaagcagc ctgatgcgct cggcaatctg gccaacgccg aacggatcgc caatccagac
      601 aacatcaagt tctcggaaaa actgcgcacg ctcttcatcg gcgaagacag cgacatgcat
      661 gtcaacaatt tcctgtgggc ctatcacatc gacagcgggc ggctctcacg cattttgtcc
      721 tgccctgcgg gtgcggagtc aaccggtctg catgcggttg acgaaatcaa cggctggacc
      781 tatatcatga gcaatttcca gcacgctggc gcctgggaaa agccgctgca cgacaaggtc
      841 agggaacggc tcgatccgct ggtgcgcgcc aattacaggg accgcttcgg cgccatggtt
      901 gcttacctca cgggcgtgca attgccggca tgaggctgtt ggaacctgtt gacggccccg
      961 caggagattg tcaacaggtc cgcgcacaac cagattgccg tgtgcgcggt ggtcggctag
     1021 catagcggtt ttgcgtatcg cgttcgcttt cctttcatgt ctcccgacga atattgccag
     1081 caaaaagctg ttcgaagcgg caccagcttt tactatagtt ttctgtttct gccgcccgaa
     1141 cgccgccgcg ccatcactgc gctgtacgcc ttttgccgcg aagtggacga tatcgtcgat
     1201 gacactgaag atgtcagcgt ggcgcgtgcc ggattgatgt ggtggcgcaa ggaaatcgcc
     1261 gccatgttcg acggcatccc gagtcatccg gtgacgcagg cgctgcgacc gcacgtggcg
     1321 gcctatgctc tcaaaggcga gcatctgcac gccatcatcg acggcatgga gatggatctg
     1381 gagcagaccc gttacctcga ttttcccggc ttgcagcgct attgctggca tgtcgccggc
     1441 gtcgtgggga tattgtcggc aagtatcttc ggcgtcactc aggacggcac gctgcggttc
     1501 gcggaaaaac tcggtctcgc ctttcagttg accaacatta ttcgcgacgt tggcgaagac
     1561 gggcgcaaag gccgtattta tctgccggtc gatgaattgc agcgcttcag cgtgagcgcc
     1621 gccgacatcc tcgctgcgcg tcacagcgaa aacttcgaga agctgatgcg ctttcaggcg
     1681 gagcgcgccc agcgtctgta tgacgaagcc tttgcgctgc tccccagaca ggatcgccgc
     1741 gcccagcgcc ccggcctgat gatggcggcg atttaccgca ctctgctcga tgaaatcggg
     1801 cacgacggtt tccatgtgct gaaccagcgc atcgcgctga cgccgatacg caaactgtgg
     1861 ctggcctgga aaacccatct ccgtggctga gaacctcgat cacatcgccg tcatcggcgg
     1921 tggttgggcc gcctgcgccg cagccgtcac actggcgcaa gccgggcggc gcgtcagcct
     1981 gttcgaatcg gcgcgcatcc tgggtggtcg cgcccgccgt gtggaaagcg acggacacgc
     2041 actcgacaat ggccagcaca ttctgctggg tgcttattgc gctaccctga agatcatgaa
     2101 agcggtcggc atcgacgccg accggatgct gctgcgcctg cccctgcaac tac
//
LOCUS       DASRDH010000044         4409 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1695613,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000044 DASRDH010000000
VERSION     DASRDH010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 4409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4409
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1695613"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2069)
                     /gene="parC"
                     /locus_tag="VFA14_01130"
     CDS             complement(<1..2069)
                     /gene="parC"
                     /locus_tag="VFA14_01130"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HZS80026.1"
                     /translation="MTEQTRLFEPAAPSDGESLTLSTFAERAYLDYAISVVKGRALPD
                     VADGQKPVQRRILYAMNQLGLNAAAKPRKSAAVVGDVLGKLHPHGDQSVYDALVRMAQ
                     DFSLRYPLIDGQGNFGSRDGDGAAAMRYTEARLTPISTLLLDEIEMGTVDFQPNYDAS
                     TEEPKLLPARLPLLLLNGASGIAVGMATEIPSHNLHEVARAAVAMIRDPRITHEELMT
                     HITGPDFPGGGQIITPRSAIADMYQNGRGSLKVRARWKIEDLARGQWQAVITELPPGV
                     SAQKVLEEIEELTNPKIKPGKKTLMPEQQAQKQSILALLDAVRDESGRNAPVRLVFEP
                     KSRNQDRIGFMHTLLAQTSLETSVPINLVMIGGDGRPRQKGLGDILGEWISFRFETIT
                     RRTRFCLRKVEDRIHILEGRETVLLNLDQVIGIIRESDQPKPALIAAFRLSDRQAEDI
                     LEIRLRQLARLEAIRIQQELTELRNEKSTLQDLLDNPASMKKRLIGEIEADSKQFGDA
                     RRTAMEEAEKAVVEQKIIDEPVTVIISQKGWIRARSGHGHDAMQFTFKAGDGMYGAFE
                     CRTIDHLLAFGANGRIYTVGVSALPNARGDGVPITTLVDTGNGNSLLHYFAGPLETPM
                     LLASDAGYGFAARLGDMQSRGKGGKAFITLDDGDLPLAPCPIPTGADAVACLSGQGRL
                     LVFRLDEL"
     gene            complement(2104..4092)
                     /locus_tag="VFA14_01135"
     CDS             complement(2104..4092)
                     /locus_tag="VFA14_01135"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008327740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HZS80027.1"
                     /translation="MAAGKTSPSDYSESSIRVLKGLEPVKQRPGMYTRTENPLHILQE
                     VIDNAADEALGGHGKNIVVTLNADGSVSIEDDGRGIPVGLHPEENIPTVEIVFTRLHA
                     GGKFDKGNSGAYVFSGGLHGVGVSVTNALASRLEVMIWREAGVHILAFADGELIEKLQ
                     SRPMTRADKKSGTRVTVWPNPTYFDTPTIPQQELQRLLRAKAVLLPGVKLALVNSKTG
                     NVQSWQYDQGLRGYLLESLAQSTGTDPLIPLFEGEQYAGADAEGFAEGEGAAWVVTWT
                     EEGNVVRESYVNLIPTPAGGTHESGLREGLFAAVKSFVEMHSLLPKGVKLLPEDVFAR
                     VSCVLSARVLDPQFQGQIKERLNSRNAVRLVSAFTRPALELWLNQHVDVGRKLAELVI
                     KQAQSRLRSAQKVEKKKSSGVAVLPGKLTDCESGDITRNELFLVEGDSAGGSAKMGRD
                     KEFQAILPLRGKVLNTWEAGRDRLFANNEIHDIAVAIGVDPHGHADTPDLSGLRYGKI
                     CILSDADVDGAHIQVLLLTLFFRHFPSLIDHGHICIARPPLYRIDAPARGKKPAQKLY
                     ALDDSELEAIEDKLRKDGLKEGAWSISRFKGLGEMNAEQLWETTMNPDTRRLLPVALG
                     EFDHIASETRFTMLMGKGEAAARRAWIEEHGNEAEADI"
     gene            complement(4110..>4409)
                     /locus_tag="VFA14_01140"
     CDS             complement(4110..>4409)
                     /locus_tag="VFA14_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015225 - biotin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015878 - biotin transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HZS80028.1"
                     /translation="WLLSAPICSLCLRLCLRHLPNADSKALLAAVFVSSLIGGMVVMY
                     PFGILGLMLAAHLSVTQAALAVLTFIPGDLVKCAICAVLVQSVMRGMPGWRLGRD"
BASE COUNT          748 a         1309 c         1394 g          958 t
ORIGIN      
        1 agttcatcga gcctgaacac cagtaagcgg ccttgtcccg acagacaagc caccgcgtcg
       61 gcgccagtcg gtatcgggca cggcgccagc ggcaggtcgc cgtcgtccag ggtgatgaag
      121 gctttgccgc ctttgccgcg actctgcatg tcaccgagtc tggcggcgaa accgtaacca
      181 gcgtcggatg ccaacagcat cggtgtctcg agcgggccgg cgaagtagtg caacaggctg
      241 ttgccgttgc cggtgtcgac cagcgtggtg atcggtacgc cgtcgccgcg cgcgttcggc
      301 agagcgctga cgccgaccgt gtagatacgg ccgttggcgc cgaaggctaa cagatggtcg
      361 atagtgcggc attcgaacgc gccatacatg ccgtcgccgg ccttgaaagt gaattgcatg
      421 gcatcgtggc cgtggccgct gcgcgcgcgt atccagcctt tttgcgaaat gatgacggtg
      481 accggttcat cgatgatctt ttgttcgacc acggcttttt cggcctcttc catggcggtg
      541 cggcgcgcgt cgccgaattg cttgctgtcg gcttcgattt caccgatcag ccgttttttc
      601 atcgaggctg gattgtcgag caagtcttgc agcgtcgatt tttcgttgcg cagttcggtt
      661 agctcctgct ggatcctgat tgcttccaga cgcgccagtt gacgcaggcg gatttccagt
      721 atgtcttctg cctggcggtc tgacagcctg aaggcggcga tcagcgccgg cttcggctgg
      781 tctgattcgc ggatgattcc gatgacctgg tcgagattga gcaggacggt ttcgcggcct
      841 tcgaggatat ggatgcggtc ctcgaccttg cgcaggcaaa agcgcgtgcg gcgcgtgatg
      901 gtttcaaaac ggaagctgat ccattcgccg agtatgtcgc cgagtccttt ctggcgcgga
      961 cggccatcgc cgcctatcat caccagattg atcgggacgc tggtttccag cgaagtctgg
     1021 gccagcaacg tatgcatgaa cccgatccgg tcctggttcc tggacttcgg ttcgaacacc
     1081 aggcgcactg gcgcattacg gcccgattca tcgcgcacgg catccagcag ggccaggatg
     1141 ctttgttttt gcgcctgctg ttccggcatc aaggtttttt tgccgggttt gatcttgggg
     1201 ttggtcagtt cttcgatttc ttccagcacc ttttgcgccg acacgcccgg tggcagttcg
     1261 gtgatgactg cctgccactg gccgcgcgcc aggtcttcga tcttccagcg cgcgcgcacc
     1321 ttgaggctgc cgcgtccgtt ctggtacata tcggcgatgg ctgatcgcgg cgtaatgatc
     1381 tggccgccgc cggggaagtc ggggccggtg atgtgcgtca tcaattcttc atgcgtgatc
     1441 ctgggatcgc gaatcatcgc caccgccgcg cgcgcgactt cgtgcagatt atgcgacggg
     1501 atttcagtgg ccatgccgac cgcgataccg gaggcgccgt tcagcagcag gaggggcagg
     1561 cgcgccggca atagctttgg ttcttcagta gaagcatcgt agttgggctg gaaatcgacc
     1621 gtacccatct cgatttcgtc gagcagcaat gtgctgattg gcgtcaggcg cgcttcggta
     1681 taacgcattg ccgctgcgcc gtcgccgtcg cgcgaaccga aattgccctg accgtcgatc
     1741 agcggatagc gcagcgaaaa atcctgggcc atgcgcacca gcgcgtcgta caccgactgg
     1801 tcgccgtgcg gatgcagttt accgagtacg tcgccgacca cggcggccga tttgcgtggt
     1861 ttggcagccg cattcaggcc gagctgattc atcgcgtaca ggatgcggcg ctgcaccggc
     1921 ttctggccat cggccacgtc gggcagggcg cggcctttga cgacagaaat ggcgtagtcg
     1981 agataggcgc gctcggcgaa agtcgatagc gtcagcgatt cgccgtcgga cggcgcggct
     2041 ggttcaaaca ggcgggtttg ttcagtcata gggggcgatg gcgttcttgc atggatgcgg
     2101 cgctcaaatg tccgcttccg cttcgttacc gtgctcttcg atccaggcgc ggcgcgcagc
     2161 ggcttcgcct ttgcccatga gcatggtgaa gcgtgtttcc gaggcgatgt gatcgaactc
     2221 gcccagggcc accggcagca gacggcgggt atcgggattc atggtggttt cccacaattg
     2281 ttccgcattc atttcaccca atcctttgaa gcgcgagata ctccaggcgc cttctttcag
     2341 gccgtccttg cgcagcttgt cttcgatggc ttccagttcg ctgtcgtcga gcgcgtatag
     2401 tttctgtgcc ggttttttgc cgcgcgccgg tgcatcgatg cgatacagcg gcggacgcgc
     2461 gatgcagata tggccgtggt cgatcaagct ggggaagtgc cggaagaaca gcgtcagcag
     2521 cagtacctga atgtgcgcac catcgacatc cgcatcggag aggatgcaga ttttgccgta
     2581 gcgcaggccg gacaggtcgg gcgtatcggc atgaccgtgc ggatcgacgc cgatggccac
     2641 cgcgatgtcg tggatttcat tattggcgaa cagacggtca cgcccggctt cccaggtatt
     2701 caagaccttg ccgcgtagcg gcaggatggc ttggaactcc ttgtcgcgac ccatcttggc
     2761 tgagccgccg gcggagtcgc cctcgaccag aaagagttcg ttgcgtgtga tatcgcctga
     2821 ttcgcagtcg gtcagtttgc ccggtagcac cgccacgccg gaggattttt tcttttcgac
     2881 tttttgcgcc gagcgcagac ggctttgcgc ctgtttgatg accagttcgg ccagcttcct
     2941 gccgacatcg acgtgctggt tcagccatag ctccagtgct ggtcgtgtga aggctgacac
     3001 tagccgcacg gcgttgcgcg agttcaggcg ttctttgatc tgcccctgga attgcgggtc
     3061 gagcaccctg gccgacagca cgcaggagac gcgcgcgaat acgtcttccg gcagcagttt
     3121 gacgcccttg ggcagcagcg agtgcatttc gacgaaactc ttcaccgccg cgaacaggcc
     3181 ttcgcgcaac cctgactcat gcgtgccgcc ggccggggta gggatcagat tgacatagga
     3241 ttcgcgcact acattgcctt cttcggtcca agtcaccacc caggccgcgc cttcgccttc
     3301 ggcaaagccc tcggcatcgg cgccggcgta ttgttcgccc tcgaacagcg ggatcagtgg
     3361 atcggtgccg gtcgactgcg ccagcgattc cagcagatag ccgcgcaggc cctggtcata
     3421 ttgccaactt tgcacgttgc cggtcttgct gttgaccagc gctagcttca cgcccggcag
     3481 cagcaccgcc ttggcgcgca gcaggcgttg cagttcctgt tgcgggatcg ttggcgtatc
     3541 gaagtatgtg ggatttggcc agacggtgac gcgggtgccg gactttttgt ctgcacgtgt
     3601 catcgggcgt gactgtagtt tctcgatcag ctcgccgtcc gcgaaggcca ggatatgtac
     3661 gccggcctcg cgccaaatca tgacttccag tcgcgaggcg agcgcattgg tcactgatac
     3721 gccaacgccg tgcagaccgc cggagaaaac ataggcgccg ctgttgccct tgtcgaactt
     3781 gccgccggca tgcaggcgcg tgaagacgat ttcgacggtt ggtatgtttt cttccggatg
     3841 caggccgacc ggaatgccgc ggccatcatc ctcgatactg acgctgccat cggcattgag
     3901 tgtgaccacg atgttcttgc cgtggccgcc aagcgcttcg tcggcagcgt tgtcgatgac
     3961 ttcctggagg atgtgcaggg ggttttcggt ccgggtatac atgcccggac gctgtttgac
     4021 cggctccagc cctttcagga cacggatgga ggactcgctg taatcggacg gggaggtttt
     4081 tccggcggcc actttgctgt ctctctgagt caatcgcggc ccaggcgcca gcccggcatg
     4141 ccgcgcatca cactttgcac cagcacggcg cagattgcgc atttgaccag atcgccggga
     4201 atgaaggtca gcacggccag cgctgcctgc gtgacgctca ggtgcgccgc cagcatcagg
     4261 ccgaggatgc cgaacggata catcacgacc atgccgccga tcagcgacga tacaaaaacg
     4321 gctgcgagca atgccttgct gtcagcattc ggcaggtggc gtaggcacag acgcaggcac
     4381 aggctgcata tcggcgcgct cagcagcca
//
LOCUS       DASRDH010000045         2591 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_202584,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000045 DASRDH010000000
VERSION     DASRDH010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202584"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="VFA14_01145"
     CDS             <1..485
                     /locus_tag="VFA14_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007880434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS80029.1"
                     /translation="ASMNGDPNGDYIVEFMPFNESHGDWREGYMSVQRSAAQVDVGAR
                     ASTQLVQRVLQGAQRDCAGHFTAMKQKDSVTNGAHGSVSGGFCDRWGGAPFGEGSLIA
                     VIEGTKNLFLVQFGWRPATENELKEYTLRITPQKLQQYLDLLNHAMLCGGQNQLICPR
                     "
     gene            complement(525..1115)
                     /gene="yjgA"
                     /locus_tag="VFA14_01150"
     CDS             complement(525..1115)
                     /gene="yjgA"
                     /locus_tag="VFA14_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008327798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HZS80030.1"
                     /translation="MPNPNRGSCGFQSREFEQEYERPSKSQLKREMTALQKLGEELVA
                     APRDRIERVPMPEDVRDAILACRQIKDHEGRRRQLQYIGKKMRTLEENDIAVIRKTLD
                     SWRGLSKADTAAMHALERRREQLLKDDQALTDLLSRHPDLDAQRLRALIRNARKEQSE
                     NRPPRAYREIFQILKQLQEQSHTAAAAENQADEREN"
     gene            1179..2543
                     /gene="pmbA"
                     /locus_tag="VFA14_01155"
     CDS             1179..2543
                     /gene="pmbA"
                     /locus_tag="VFA14_01155"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013233860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HZS80031.1"
                     /translation="MSDTVFTHSQDQLKQLAQDVLRYAREKGASDAAVDISEGKGLSV
                     AVRKGKVETIEQNMDKAVGVTVYMGNGRNVRCGHASTSDFAQQALRQTVAAAYDIARF
                     TAIDDCAGLPDAENLEMHVRDLKLCVPWLITTKEALALARRCEAAAFAVDRRITNSEG
                     ASVHAQQSHFVTANSRGFIGGYPFSRHTISVAPIAGQGANMQRDDWYSSMRDPDKLAK
                     PEAIGRYAAERALARLHARKLSTRKCPVLFEAPLAIGLLGAFVQAVSGGALYRKSTFL
                     LDSLHKQVFPEHIRILEDPYVIGGIGSAPFDNEGVKTQRREVVSGGVVQGYFLSTYSA
                     RKLGMQTTGNAGGSHNLSISSSLTAATDTFEGVLKKLDTGLLVTELMGQGVNYVTGDY
                     SRGASGYWVEQGVIQYPVEEITIAGNMKDMLRRIVAIGNDTLMRGAKETGSILIENMT
                     VAGD"
BASE COUNT          512 a          694 c          818 g          567 t
ORIGIN      
        1 tggcgtctat gaatggcgat ccgaacggcg actatattgt cgagttcatg ccgttcaacg
       61 aaagccatgg ggattggcgc gaaggttata tgtcggtgca gcgcagcgca gcgcaggtcg
      121 atgtcggcgc gcgtgcatcg acacagctcg ttcagcgtgt gctacaaggg gcgcaacgcg
      181 actgtgcggg tcactttacg gcgatgaagc aaaaagacag cgtgaccaat ggcgcccacg
      241 gctcggtcag cggcggcttt tgtgatcgat ggggcggcgc gccattcggg gaaggcagtc
      301 tcatcgccgt catcgagggg acgaaaaacc tgttcctggt gcagttcggc tggcggccgg
      361 ccaccgaaaa cgagctgaag gaatataccc tgcgcattac gccgcaaaag ctgcaacagt
      421 atctggacct gttgaaccac gctatgctgt gcggcgggca gaatcagttg atttgtcccc
      481 ggtagaatct gttctgcacc agaccgatca gtaaagcgcc gggatcagtt ctcccgttca
      541 tcggcttgat tctctgctgc tgcggcagta tgggactgct cctgcaactg cttgaggatc
      601 tggaagattt ctcgatatgc cctgggcggc ctgttttcgg actgttcctt gcgggcgttg
      661 cggatcaggg cgcgcaggcg ttgcgcatcc agatcggggt gccgggacag caggtcggtc
      721 agggcctgat cgtctttcaa taactgctca cggcggcgtt ccagagcgtg catcgccgcg
      781 gtatccgcct tggacaaacc acgccagcta tcgagggttt ttcggatgac ggcgatgtca
      841 ttctcttcca gcgtgcgcat ttttttgccg atatattgca actggcgccg ccggccttcg
      901 tgatccttga tctgccggca ggcgaggatg gcgtcgcgca catcttccgg catcggcacg
      961 cgctcgatgc ggtcgcgcgg cgcggcaacc agttcttcgc ccagtttctg caaggcagtc
     1021 atttcgcgtt ttagctggga tttggaggga cgttcgtatt cctgctcgaa ttccctagac
     1081 tggaagccgc aggatccgcg attgggattt ggcatggtgc agataaataa gctaaacggc
     1141 tgctatgata cctgctttat ccgcatttcg aagaaatcat gagcgatacc gtatttaccc
     1201 atagccaaga ccaattgaaa cagctggcgc aggatgtatt gcgttatgcc agggaaaagg
     1261 gcgcctccga tgcggcggtc gatatcagtg agggtaaggg actgtcggta gcggtgcgta
     1321 agggcaaggt cgagaccatc gagcaaaata tggataaagc ggtcggcgtg actgtgtata
     1381 tgggtaacgg tcgcaatgtc cggtgcggcc atgccagtac gtcggatttt gcgcagcagg
     1441 cgctcaggca aaccgtggca gcggcttacg acatcgcgcg cttcaccgcg atcgacgatt
     1501 gcgctggcct gcccgatgca gaaaatctgg agatgcatgt gcgcgatctc aagctgtgcg
     1561 tgccgtggct gatcacgacg aaagaagcgc tggcgctggc gcggcgttgc gaagccgccg
     1621 ctttcgcggt cgacaggcgc atcaccaata gtgaaggcgc cagcgtccac gcccagcaat
     1681 cgcatttcgt caccgccaat tcgcgcggtt tcatcggcgg ttatcctttt tcgcgccata
     1741 ctatttcggt tgcgccgatt gccggtcagg gcgccaacat gcagcgcgat gactggtatt
     1801 cgtccatgcg cgaccccgat aaactggcca agccggaagc catcggccgt tatgccgccg
     1861 aacgcgcgct ggcgcgtttg catgcgcgca agttgtcgac gcggaagtgt ccggtgttgt
     1921 tcgaggcgcc gctggcgatc ggcctgctgg gggcttttgt gcaggctgta tcgggtggcg
     1981 cgctctatcg caagtcgact tttttgctcg attcactgca taaacaagta ttcccagaac
     2041 atatccggat tctcgaagat ccgtatgtca tcggtggcat tggttcagcg cccttcgata
     2101 atgaaggcgt gaagacgcag cgccgcgagg tcgtcagcgg tggcgtggtg caaggctatt
     2161 tcctgtcgac gtattcggcg cgcaaactgg gcatgcagac aaccggcaat gcgggcggtt
     2221 cgcataatct gtcgatcagc tcgtcgctga ctgcggctac ggacacgttc gagggagtgc
     2281 tgaaaaaact cgataccggc ctgctggtga cggaactcat ggggcagggc gtgaattacg
     2341 tgaccggcga ttattcacgc ggcgcatcgg gttactgggt ggagcagggg gtgatccagt
     2401 atccggtcga agaaatcact atcgccggca acatgaagga catgctgcgc cggattgtcg
     2461 ccatcggtaa tgacacgttg atgcgcggcg ccaaggaaac cggttccatc ctgatcgaga
     2521 acatgacggt cgcgggtgat tgatgcggtt tcggccagca acttgctggc tcgacgcagt
     2581 tatctacttc a
//
LOCUS       DASRDH010000046         4140 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1121422,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000046 DASRDH010000000
VERSION     DASRDH010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 4140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4140
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1121422"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="VFA14_01160"
     CDS             <1..498
                     /locus_tag="VFA14_01160"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HZS80032.1"
                     /translation="VFHSQEAFMQAFKAGELDRDFVAVLSFQGPRANGMPELHALTPA
                     LGILQDRGRHVALVTDGRMSGASGKVPAAIHVTPEVLAGGALGCVRTGDMIRLDADAG
                     ILEALVPAAEWAARASEMIDLSGNQTGMGRELFRVFRASVSTAEEGGASFGLPSLLEN
                     EKVTV"
     gene            495..1157
                     /gene="eda"
                     /locus_tag="VFA14_01165"
     CDS             495..1157
                     /gene="eda"
                     /locus_tag="VFA14_01165"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013233129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - 4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0009084 - glutamine family amino acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HZS80033.1"
                     /translation="MKNLLEIMHTSAVIPVIAIDKPEHAVPLARALVTGGIHVLEVTL
                     RTRHGLPAIRAITEQVPDAIVGVGTLTSPEEFTAARDAGAVFGVSPGLTAALIAAAGS
                     SGLPLLPGVMTPSEVMAARAAGFRQLKLFPAVLAGGVAMLNAIAGPLADVTFCPTGGI
                     SQETAPQFLACQNVACVGGSWLTPKNAIDSGDWSRITALAAAASALKKMKQEPVHGQQ
                     QA"
     gene            complement(1154..3493)
                     /locus_tag="VFA14_01170"
     CDS             complement(1154..3493)
                     /locus_tag="VFA14_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HZS80034.1"
                     /translation="MLPSIEQRLALELVARPAQVAAAIALLDEGATVPFIARYRKEAT
                     GALDDTQLRLLEERLRYLRELESRRATVMASIEEQGKMTPELRDAIVHAEDKTRLEDL
                     YLPYKPKRRTRAQIAAEAGLTELADALLNDPMLNPEEEAVRYIKPSFTTGNGDNPGVA
                     DTKAALDGARQILMERFSEDAQLLQSLRAYLAEHGVVASKVLEGKQHAGEKFADYFDH
                     SEAIAAMPSHRALALFRGRREEILTVTLRLDSEAEKPKWDAPHNPCESRIAARFGVND
                     KGRPGDRWLADTVRWAWRVKVFVHLETELMSGLRERSEAEAINVFARNLKDLLLAAPA
                     GPRVTMGLDPGLRTGVKVAVVDATGKVLDTATVFPHQPRNDWDGSLRTLAELAEQHKV
                     TLISIGNGTASRETDRLAQDLIRLRPELKLTRVVVSEAGASVYSASEFASKELPELDV
                     SLRGAVSIARRLQDPLAELVKIDPKSIGVGQYQHDVGQSQLARSLDAVVEDCVNAVGV
                     DVNTASAPLLARVSGLNAAVAQSIVSYRDLKGRFASRSALRDVPRLGDKTFEQAAGFL
                     RVMHGDNPLDTSAVHPESYPLVKKILADINRDIKSVLGEPALLKALDPSRYADERFGV
                     PTVTDILKELEKPGRDPRPEFTTATFRDGIETIGDLHPDMLLEGVVTNVASFGAFVDI
                     GVHQDGLVHISALSNTFVKDPHTVVKAGQVVKVKVLEVDEKRKRIALTMRLSDSAPMA
                     GSRPGQRGSRDDARRLSQHQGRPAPTNNVMAAAFAKLKG"
     gene            3832..>4140
                     /gene="pth"
                     /locus_tag="VFA14_01175"
     CDS             3832..>4140
                     /gene="pth"
                     /locus_tag="VFA14_01175"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013236115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HZS80035.1"
                     /translation="MSIRLIAGLGNPGPEYQQTRHNAGFWLVDNLAGGTRLTRETRFD
                     ALVAKTCIGGREVWLLEPQTFMNRSGLSVGALARFYKIAPDEVLIVHDELDLPPGSAR
                     I"
BASE COUNT          765 a         1265 c         1260 g          850 t
ORIGIN      
        1 gtcttccatt cacaggaagc tttcatgcag gctttcaagg ccggtgaact cgaccgggac
       61 tttgtcgccg tgctgagctt tcagggaccg cgcgccaacg gcatgccgga actgcacgca
      121 ttgacgccgg cgctgggcat actgcaagac aggggacgtc atgtggcact ggtcaccgat
      181 ggccgtatgt cgggcgcttc gggcaaggtg ccggcggcga tccacgtgac gccggaagtc
      241 ctggccggcg gtgctttggg ttgtgtacgc actggcgata tgatccgtct ggatgccgac
      301 gccggtattc tggaagcgct ggtgccggca gccgaatggg ccgcgcgcgc atcggaaatg
      361 atcgacctgt caggcaacca gactggcatg gggcgtgaac tgttccgtgt tttccgcgca
      421 tcggtgagca cggcggaaga gggcggcgcc agtttcggtc tgccttcttt actggaaaac
      481 gagaaagtaa cagtatgaaa aacctcctgg aaatcatgca tacctcggcg gtcatccctg
      541 tgattgccat cgacaaaccg gaacatgccg tgccgctggc cagagcgctg gtgacgggcg
      601 gcatccacgt actcgaagtg accttgcgca ccaggcacgg cctgccggcg attcgtgcca
      661 ttactgaaca ggtgcctgac gccatcgttg gcgtcggcac gttgaccagc ccggaagaat
      721 tcactgctgc acgcgatgcg ggtgccgtgt tcggcgtgtc gccgggcttg acagcggcgc
      781 tgatcgccgc cgccggatcc agcggcttgc cgctgttacc aggtgtgatg acgccgtcgg
      841 aagtgatggc ggcgcgcgca gccgggtttc gtcaattgaa actgttcccg gcggtactgg
      901 cgggcggcgt cgccatgctc aacgcgattg ccggtccgct ggccgacgtg acattctgcc
      961 cgaccggcgg catctcgcag gagacggcgc cgcaatttct ggcgtgccaa aacgtagcct
     1021 gcgtcggcgg ctcctggctc acgccgaaaa acgctatcga cagcggtgac tggagcagga
     1081 tcacggcact cgccgcagcc gcaagtgcgt taaagaagat gaagcaggag cctgttcatg
     1141 ggcagcagca agcctagccc ttgagttttg cgaatgccgc cgccatgaca ttgtttgtcg
     1201 gcgccggccg gccctggtgt tgcgacaggc ggcgcgcgtc gtcacgactg ccgcgctgcc
     1261 cgggcctgct accggccatc ggcgcgctgt cggacagacg catggtcagc gcgatacgtt
     1321 tgcgcttttc atcgacttcc aataccttca ctttgaccac ctgaccggcc ttgacgacgg
     1381 tatgcggatc cttgacgaaa gtattcgaca aggcggagat atgcaccagg ccatcctgat
     1441 gtacaccgat gtcgacgaat gcgccaaagg atgccacatt ggtcacgacg ccttccagaa
     1501 gcatgtccgg atgcaagtcc ccgattgttt cgatgccgtc cctgaatgtc gctgtagtga
     1561 attcaggacg cggatcgcgc cccggctttt ccagctcttt caggatgtcg gtcacggtcg
     1621 gtacgccgaa tctttcatca gcgtatctgg agggatcgag cgctttcagc aaagccggtt
     1681 cacccaacac acttttgata tccctgttga tgtcggccag aatttttttc accagcggat
     1741 acgattccgg gtgaaccgct gaagtgtcca gaggattgtc gccatgcatc acgcgcagga
     1801 aacctgctgc ctgttcgaac gtcttgtcac ccagacgcgg cacgtcgcgc agcgcggagc
     1861 gcgaagcgaa cctgcccttc aggtcgcgat acgacacgat gctttgcgcg accgcggcgt
     1921 tcaaccccga cacgcgcgcc agcaatggcg ccgatgcggt gttgacatcg acgccgacgg
     1981 cattcacgca atcttcgacg acggcgtcca gcgagcgcgc cagttgcgac tggccgacgt
     2041 catgctgata ctggccgaca ccgatggact tcggatcgat tttgaccagt tccgccagtg
     2101 gatcctgcaa acggcgcgca atcgacacgg cgccacgcag cgatacatcc agttccggca
     2161 attctttcga agcaaattcg gaggccgaat acaccgaagc gccggcttcc gacacgacga
     2221 ccctggtcag tttcagttca gggcgcagtc tgatcagatc ctgcgccagc ctgtcagtct
     2281 cgcgcgaggc agtgccgttg ccgatggaga tcagcgtaac cttgtgttgc tccgcgagtt
     2341 cagccagcgt gcgcagcgaa ccatcccagt cgttgcgcgg ctggtgcggg aaaaccgttg
     2401 cggtgtcgag aaccttgccg gtcgcatcga cgactgccac cttgacgccg gtacgcaggc
     2461 cggggtccag gcccatcgtg acgcgcgggc cggctggcgc ggccagcagc aaatccttca
     2521 ggttgcgcgc gaagacgttg atcgcctcgg cttcactacg ttcgcgcagg ccgctcatca
     2581 gctcggtttc cagatgcacg aaaaccttca cgcgccaggc ccaacgcacg gtatcggcca
     2641 gccacctgtc gcccggacgg cccttgtcgt tcacgccgaa gcgggcggca atgcggcttt
     2701 cgcagggatt atgcggcgca tcccacttcg gtttttctgc ttcgctgtcc agacgcagcg
     2761 tcacggtcag tatttcttcg cgacggccac ggaacagcgc cagcgcgcga tgtgacggca
     2821 tggcggcgat ggcttcggaa tgatcgaaat agtcggcaaa tttttcaccg gcatgctgct
     2881 tgccttcgag cactttcgat gcgactacac cgtgctcggc caggtacgcg cgcagtgact
     2941 gcaacagttg tgcatcttca gaaaaacgct ccatcagaat ctggcgtgcg ccgtccagcg
     3001 cggctttggt atcggccacg cccggattgt cgccgttgcc ggtagtgaag gacggtttga
     3061 tgtacctgac ggcttcttct tccggattca gcatcggatc gttcagcagc gcatcggcca
     3121 gttcggtcag accggcttca gcggcgatct gcgccctggt gcggcgcttc ggcttgtacg
     3181 gcaggtacaa gtcttcgagg cgcgtcttgt cttcggcatg gacgatggca tcgcgcaatt
     3241 ccggcgtcat tttgccttgc tcttcgatcg acgccatgac cgtcgcgcgg cggctttcca
     3301 gttcacgcaa ataacgcagg cgctcttcca gcagacgtag ttgtgtgtcg tccagcgcgc
     3361 cggtggcttc cttgcggtaa cgcgcgataa acggcacggt cgcgccttcg tcgagtaagg
     3421 cgatcgcggc agcgacctgc gctggcctgg cgacgagttc gagagctaag cgttgttcga
     3481 tggaaggcag catgacaatc aaatccggaa attgaaaaac aagtgctgga tgatacgcaa
     3541 ttcgggcgca tgcgccaatc ctgagaacct gttgccgatc tcctgcgggg ccgtctggtc
     3601 gctctttcag gcggtctgct gcgttgccaa tcctcgccgg gccaccagcc cgcctgcggt
     3661 ttgcgccttg catcccatcc tgaaaaaacc accggacgac tcccgatgag atcgtcaaca
     3721 ggttctgaga accgccacgg atttccggcg ataaaccgtc aaggatcggg cgcggcgatt
     3781 cgttatccgc gataatcgtt gctttcatta tctgtcgtta tcttatccag tatgtctatc
     3841 cgtcttatcg ccggtcttgg caatcccggc ccggaatatc agcaaacccg ccataatgca
     3901 ggcttctggc tggtagacaa tcttgccggt ggcacgcgcc tcacgcgtga aacacgcttt
     3961 gatgcgctgg tcgcgaaaac ctgcatcggc ggtcgcgaag tctggttgct ggagccacag
     4021 actttcatga accgttccgg cctgtcggtc ggcgcgctgg cgcgtttcta taagatcgcg
     4081 ccggatgaag tgctgatcgt ccatgacgaa ctggatctgc cgcccggcag cgccaggatc
//
LOCUS       DASRDH010000047         2855 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_202286,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000047 DASRDH010000000
VERSION     DASRDH010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202286"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..872
                     /locus_tag="VFA14_01180"
     CDS             <1..872
                     /locus_tag="VFA14_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011354816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZS80036.1"
                     /translation="GFDPAKKYAAIICAHPISSCKEQTAGDIYGEGLTREGFVTLAFD
                     ASTQGASGGEPRYGEDPGTRVEDFRCAVDYLVTLPYVDEERIGVLGVCGGGAYAANVA
                     MTEKRIKAVGTVVATNYGRMMRDGDNSPDAALKTLEAIGKQRTAEARGAKPLIVGYIP
                     FASQAEREKAGVNDVDMVQAIDYYLTPRGQQPGSPNKLRLTSTGAALGWDAFHLAEKL
                     LTQPLHVVFGGVPGAFGSYSDGQDLYRRARSADKTMQIVPGACHYDLYDQPEATGQAL
                     AQLALFFNRHLRA"
     gene            1226..>2855
                     /gene="lepA"
                     /locus_tag="VFA14_01185"
     CDS             1226..>2855
                     /gene="lepA"
                     /locus_tag="VFA14_01185"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017876442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HZS80037.1"
                     /translation="MNNIRNFSIIAHIDHGKSTLADRIIQLCGGLSSREMEAQVLDSM
                     DIERERGITIKAQTAALQYKALDGNIYHLNLIDTPGHVDFSYEVSRSLSACEGALLVV
                     DASQGVEAQTVANCYTALDLGVEVVPVLNKIDLPSADPDNARREIEEVIGIDAADATL
                     CSAKTGVGMQDVIEALIAKVPPPKGDPDAPLQALIIDSWFDNYVGVVMLVRIVNGILR
                     PKGRIFLMATGSMHLTESIGVFTPKAQSRETLEAGQVGFIIAGIKELKAAKVGDTVTL
                     AANRAAEPLPGFREVQPQVFAGLFPVEANQYDALRDSLEKLKLNDAALQYEPEVSQAL
                     GFGFRCGFLGLLHMEIVQERLEREFDMDLITTAPTVVYEVVLRDGSILMVDNPSKMPE
                     PSRIEEVREPIITVNLYLPQEYVGSVMTLCTQKRGVQINMSYHGKQVQLTYEMPMAEI
                     VLDFFDRLKSTSRGYASMDYAFKEYRSSDVVKVDMLINSEKVDALAIIVHRAASQSRG
                     RAVAARMRELIPRQMFDVAIQAAIGATIISRENVK"
BASE COUNT          576 a          818 c          870 g          591 t
ORIGIN      
        1 aaggcttcga ccccgccaag aaatacgcgg ccatcatttg cgcccacccc atcagcagtt
       61 gcaaggagca gaccgctggc gatatctacg gtgaagggct gaccagggaa ggcttcgtca
      121 ccctagcctt cgatgcctcc actcagggcg cgagcggtgg cgagccgagg tacggtgaag
      181 accccggaac gcgcgtggaa gacttccgct gcgccgtgga ctatctggtc acgctgccct
      241 atgtggacga ggagcgcata ggcgtgctgg gtgtgtgcgg cggcggcgcc tatgcggcca
      301 atgtcgccat gaccgaaaag cgcatcaagg ccgtgggcac ggtggtggcc accaactacg
      361 gccgcatgat gcgcgacggc gataattcgc cggacgctgc actcaaaacg ctggaagcca
      421 tcggcaagca gcgcaccgcc gaggcacgcg gcgccaagcc cctgatcgta ggctacatcc
      481 ctttcgctag tcaggccgag cgcgagaagg cgggcgtcaa cgacgtggac atggtgcagg
      541 ccatcgacta ctatctgacg ccgcgtggcc agcagcccgg ctcgcccaac aagctgcgcc
      601 tgaccagcac gggcgcggca ctgggctggg atgcctttca cctggctgaa aagttgctga
      661 cgcagccgct gcacgtggtt tttggtggcg tgccgggcgc tttcggctct tactcggatg
      721 ggcaagatct gtatcgccgc gctcgttcgg cggacaagac gatgcagatc gtgccgggcg
      781 cctgccacta tgacctgtat gatcagccag aggccaccgg ccaggcactg gcccaactgg
      841 cgctattctt caacagacat ctgagagctt gaggaggcgt tcagtggccc gcgtgaaatg
      901 ccactcctcg gccaaaacga cgaacgagca tccgtcactg cccagcatgg acaagctgtt
      961 tcagcctgac gctggctggg acgaccagcc gattgccaca ggtcggattt tcttcgtcat
     1021 ttccttggtt cctttccagc agtatcgctg gcttaggccc cggtctctcc acttggccgg
     1081 ctgtccgaat ttgcgcggcc acccttggtt tcggggtaga atgccaagct gttgtcagga
     1141 tgagatcgtg agctcgttct aaaggcgctc aattgggaga cgctcccatc ctggggtgcc
     1201 ttttttcatc gtttcccgca atttaatgaa caatattcgt aatttttcga tcattgccca
     1261 cattgaccat ggtaaatcca cgctcgctga tcgcatcatt caactttgcg gcggtctctc
     1321 cagtcgcgag atggaggccc aggttctgga ttcgatggac atcgagcgcg agcgcggcat
     1381 cacgatcaag gcgcagactg cggccttgca gtacaaggcg ctcgacggca atatctacca
     1441 tctcaacctg atcgacacgc cgggccatgt cgacttcagt tatgaagtca gccgctcgct
     1501 gtcggcgtgc gaaggcgcct tgctggttgt agatgcatcc cagggcgtcg aagcgcagac
     1561 cgtggcgaat tgctataccg cgctcgatct gggtgtcgaa gtggtgccgg tgctcaacaa
     1621 gatcgacctg ccgtctgccg atccagacaa tgccaggcgc gagatcgagg aagtcattgg
     1681 catcgatgcc gccgatgcga cgctatgctc ggccaagact ggtgtgggca tgcaggacgt
     1741 catcgaggca ctgattgcca aggttccgcc gccgaagggc gatccggatg caccattgca
     1801 ggcgttgatc atcgattcct ggttcgataa ttatgtcggc gtggtcatgc tggtacgtat
     1861 cgtcaatggc attttgcgcc cgaagggcag gattttcctg atggctaccg ggtccatgca
     1921 tctgactgaa agcattggcg tttttacgcc caaggcgcag tcgcgtgaaa cgctggaggc
     1981 agggcaggtc ggtttcatca tcgctggcat caaggaattg aaagcagcga aggtcggcga
     2041 tactgtcacc ctggcggcga accgggctgc cgaaccgttg ccgggtttca gggaagtgca
     2101 gccacaggtg tttgccggcc tgtttccggt cgaggccaat caatacgacg ccttgaggga
     2161 ttcgctcgaa aaactcaagc tcaacgacgc cgcgcttcag tatgagccgg aagtctcgca
     2221 agccctgggt ttcggtttcc gctgcggctt tctcgggttg ctgcatatgg aaatcgtgca
     2281 ggaacgcctc gagcgcgaat tcgacatgga tctgatcacg acggcgccga cggtcgtgta
     2341 tgaggtcgtg ctgcgtgacg gcagcatcct catggtcgac aatccgtcaa agatgccgga
     2401 gccgtccagg atcgaagaag tgcgcgagcc tatcatcacg gtcaatctct acctgccgca
     2461 ggaatacgtc ggttcggtga tgacgctgtg tacgcaaaaa cgtggcgtgc agatcaatat
     2521 gagttatcac ggcaaacagg tgcagctgac ttacgaaatg ccgatggcgg aaatcgtgct
     2581 cgatttcttc gaccgtctaa aatcgacctc gcgcggctat gcgtcgatgg attatgcatt
     2641 caaggagtat cgctcatccg acgtggtcaa ggtcgacatg ttgatcaaca gtgagaaggt
     2701 cgatgcgctg gcgatcattg tgcatcgcgc tgccagccag tcccgcggtc gcgccgtggc
     2761 ggccaggatg cgtgaactga tcccgcgtca gatgttcgat gtcgcgattc aggctgccat
     2821 cggcgccacc atcatttcgc gtgaaaatgt caaag
//
LOCUS       DASRDH010000048         5827 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_541630,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000048 DASRDH010000000
VERSION     DASRDH010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 5827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5827
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541630"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="VFA14_01190"
     CDS             <1..382
                     /locus_tag="VFA14_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883382.1"
                     /note="ligates
                     L-alanyl-gamma-D-glutamyl-meso-diaminopimelate to
                     UDP-N-acetylmuramic acid for reincorporation into
                     peptidoglycan; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HZS80038.1"
                     /translation="DFAHHPTAIRTTVDGLRQKIGKARLLAVLEPRSNTMKLGIMKDA
                     LPASLKEADLVFAYGAKGNGKDAPGWDLAQALSPLGAKAQVFGDLGALVRAIRQTARA
                     GDHVLVMSNGSFGGVHQKLLDALA"
     gene            453..2474
                     /locus_tag="VFA14_01195"
     CDS             453..2474
                     /locus_tag="VFA14_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HZS80039.1"
                     /translation="MNVFFEESGDFKAGVVLSQQGEAYQVAMASGKRSKVKSRDVMLQ
                     FAAPDPVQLLEQAQGIARDIDLDFLWEVAGEDEFGFAELGMEYFGHAPLPHEAAGLLL
                     RLHAAPIHFYKKGKGRYKAAPQASLQAALAGLERKKQQAQIQAQYVEELKSGRLPASF
                     RPLPLLFRPDRNSIEFKALDAACKELQTTSQRLMLSTGGLASPKELHEARFLFEFFPR
                     GSGFPEVSMPAVPADLPLADVQAFSIDDVTTTEIDDALSVSRLDDGKVRVGIHIAAPG
                     LGIKCGDALDAIARERMSTVYMPGEKITMLPDALVDAFTLDEGKNCPALSLYVTLDPR
                     DWSVISTETRAERVPIVANLRHNALDALVTEEALAADSGDYPHKNDISLLWQWVQVLE
                     RGRMARRESFGLHPEQNNRVDFSFYVDAGVVTITRRRRGAPLDKLVAELMIFANSTWG
                     KLMADHGVPGIYRAQGGGSGGWATRMQVRMVTHAAPHQGLGVDQYAWSTSPLRRYTDL
                     VNQWQILACLEHGVTAPLAAPFKPRDADLFAIVSAFDSAYSAYAGFQATMERYWCLRW
                     LAQEQVRQVDAVVLKEGVLRLADIPLVISLPGMPQLARGLQVRLDLLRWDEVDLSIEA
                     RLLEIPAAQPAADAASMDDDALLQGMDGAGGAGESGNGTAADTSAIAAE"
     gene            2526..3416
                     /locus_tag="VFA14_01200"
     CDS             2526..3416
                     /locus_tag="VFA14_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB C-terminal domain-containing protein"
                     /protein_id="HZS80040.1"
                     /translation="MKLFADHRFLAIAVTGSVLIHAVLLGVHFAAPDTLRPKPADPGL
                     EVILVNARHDKEPLKADAVAQANLDGGGNASEGRASSPLPDMRKLKSGDGIEAAQRTV
                     AELEARQQKVLAQMNQLTPTVAVPEREKTRKVPRQSSDRDLSDAAREMARLEAEVARN
                     IEDYNRRPKKTQITARTRKVDYAFYYKRMQEKIEILGTLNFPHRDGRKLYGELLVYIP
                     VFQDGTIYEKEGGARVERSSGNAALDSAALAIVQRSAPFGRFPKNMRTTDKDDVWEII
                     TRFRFTHDENLRIDADSSHN"
     gene            3434..4237
                     /gene="aroE"
                     /locus_tag="VFA14_01205"
     CDS             3434..4237
                     /gene="aroE"
                     /locus_tag="VFA14_01205"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HZS80041.1"
                     /translation="MDAYAVIGNPIAHSKSPEIHARFAAQTGQELSYECLPAPLSGFR
                     AVVQSFIADGGKGANVTLPFKLEAYGLAGRLTERARLAGAVNTLKFEDGCILGDNTDG
                     AGLVRDIVRNAGVALSGKRVLLLGAGGAARGALLPLLEQHPSELVLSNRTHTRARELA
                     TQFATYGNLTATEHAGLRGAFDVVINATSASLAADVPPLPAKVFLPTTLAYDMMYGKQ
                     PTVFMEFARRHGAIARDGLGMLIEQAAESFFLWRGVQPQTAEVFAALRA"
     gene            4289..5035
                     /gene="mtgA"
                     /locus_tag="VFA14_01210"
     CDS             4289..5035
                     /gene="mtgA"
                     /locus_tag="VFA14_01210"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HZS80042.1"
                     /translation="MKLLRQSFLWLIVLSASLLLLLQLYFFAQIWWWVDHNPASTSFM
                     CHQLSLLQEKNPHARLQFQWVPYSRISNHLKRAIIASEDSRFSGHEGVDWEALQQAYE
                     KNVRKGRVVAGGSTITQQLAKNLFLSGERSYARKAQEFVIAYMLEFLMDKQRIFEIYL
                     NVVEWGNGVFGAEAAARHYYGSSAASLGPSQAARLAVMLPRPRYYDRNPDSAYLARRT
                     ALILRRMGAAELPASPARSSTNNKLRLESA"
     gene            5032..>5827
                     /locus_tag="VFA14_01215"
     CDS             5032..>5827
                     /locus_tag="VFA14_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013232448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HZS80043.1"
                     /translation="MINVFVLQNGRLNQVNIDSRSDLENVAPVWVDLTEPTDDERAWV
                     KSIYGVALPDEDEFSDIEASARYFEAENGDLHLRTDFLFESDDTASATMTVAFILARN
                     ILFSMHAEDLPVFRLVRMRARSRPGSIGDYRDVLLNLYETDAEYSADALEGIYQRLEI
                     VSHSVLKKSLSDQAAAAVLNTIAHEEDLNGRVRRNMMDTRRAVSFLMRGRLLNTEQFE
                     DARQILRDIESLDGHTAFLFDKINFLMDATVGFININQNKIIKIFSV"
BASE COUNT         1146 a         1626 c         1833 g         1222 t
ORIGIN      
        1 cgacttcgcc caccacccga ccgcgatccg gaccacggtt gacgggctgc ggcagaagat
       61 aggcaaggcg cgcctgctcg ccgtgctgga gccgcgctcc aacaccatga agctcggcat
      121 catgaaagac gctctgccgg ccagcctgaa agaagccgat ctggtgttcg cttacggcgc
      181 caaaggcaat ggcaaggatg cgcctggctg ggatctggcg caggcgttgt cgccgctggg
      241 tgcgaaagcg caggtcttcg gcgatctcgg cgcgctggta cgcgccatca gacagactgc
      301 gcgtgccggc gaccatgtgc tggtcatgag caatggtagc tttggcggcg tgcatcagaa
      361 gttactggat gcgctggcat gacagcgtca tgctggcggc caatgttttt ttacccgctc
      421 tcagcaagct gatggcattg aatgtgcaag caatgaatgt attctttgaa gaatccggcg
      481 atttcaaggc tggcgtcgtg ctgtcacagc aaggtgaggc ctatcaggtc gcaatggcgt
      541 ccggcaagcg cagcaaggtc aagtccaggg acgtgatgct gcaattcgcc gcgccggatc
      601 cggtacagtt gctggagcag gcgcaaggca tcgcacgtga catcgatctc gatttcctgt
      661 gggaagtcgc cggtgaagac gaatttggtt tcgccgaact gggtatggag tatttcggcc
      721 atgcgccgct gccgcatgaa gcggccggct tgctgctgcg tctgcatgcg gcgccgatcc
      781 atttctacaa aaagggtaag ggacgctaca aggcggcgcc gcaggcatcg ttgcaggccg
      841 cgctggccgg tctggagaga aaaaagcagc aggcgcagat acaggcgcag tatgtcgaag
      901 aactcaaatc cggcaggctg cccgcatcgt tccggccgct gccgttgctg tttcgtcccg
      961 acaggaacag tatcgaattc aaggcgctcg acgccgcctg caaggaattg cagaccacgt
     1021 cgcagcgtct gatgctctcc accggtggtc tggcttcgcc gaaggagctg catgaggcca
     1081 gattcctgtt tgaatttttc cccaggggca gtggcttccc cgaggtgagc atgcctgccg
     1141 tgccggctga tttgccgctg gcggatgtgc aggcgttctc catcgatgat gtcactacaa
     1201 ccgaaatcga tgatgccttg tcggtgtccc ggctcgatga tggcaaggtg cgcgtcggca
     1261 ttcacatcgc cgcgcctggc ctcggcatca agtgcggtga tgcgctcgat gcgatcgcgc
     1321 gtgagcgcat gtcgacggtc tatatgcccg gtgaaaaaat caccatgctg ccggatgcgc
     1381 tggtcgatgc tttcactctc gatgaaggca aaaactgccc ggcgctgtcg ttgtacgtca
     1441 cgctcgatcc gcgggactgg agtgtgatct cgaccgagac gcgcgccgaa cgcgtgccga
     1501 tcgtggccaa cctgcgccac aacgcgctgg atgcgctggt gaccgaagaa gcgctggctg
     1561 ccgacagtgg tgactatccg cacaaaaatg acatcagctt gctgtggcaa tgggtgcagg
     1621 tgctggagcg gggccgcatg gccaggcgcg aaagttttgg tctgcatccc gagcaaaaca
     1681 accgcgtcga tttcagcttt tacgttgacg ccggtgttgt cacgatcacc cgccgcaggc
     1741 gtggcgcgcc gctggataag ctcgtcgccg agctgatgat cttcgctaac agcacctggg
     1801 gcaagctcat ggccgatcat ggtgtgccgg gcatttaccg cgcgcagggc ggcggcagtg
     1861 gcggctgggc taccaggatg caggtgcgca tggtcacgca cgcggcgccg catcaggggc
     1921 ttggcgtcga tcaatatgcc tggagtacgt cgccgttgcg tcgttacacc gatctggtca
     1981 atcaatggca gatcctggcc tgcctcgaac acggcgtcac ggcgccgctg gcggcgccgt
     2041 tcaagccgcg cgacgctgat ctgtttgcga tcgtgtcggc gttcgactcg gcctactccg
     2101 cttatgccgg ttttcaggcc actatggagc gttactggtg tctgcgctgg ctggcgcagg
     2161 aacaggtgcg ccaggtcgat gccgtggtac tcaaggaggg agtgctgcgt ctggccgaca
     2221 ttccgctggt gatcagcttg ccgggcatgc cgcaactggc gcgcggcttg caggtcaggc
     2281 tggatctgtt gcgctgggat gaagtcgacc tgagcatcga agcgcgtcta ctggaaattc
     2341 cggcagcgca gccggctgcc gatgccgcgt ctatggacga tgacgccttg ttgcaaggca
     2401 tggatggcgc tggcggagca ggcgagagcg gcaatggcac agctgcggat acttctgcta
     2461 ttgctgcgga atgaatccgg ttttggcgta aaatcgccct gttttcatca ggtgtcgatg
     2521 cttttgtgaa actgttcgcc gaccatcgtt tcctggccat cgccgtgact ggttccgtgt
     2581 tgatccatgc ggttttgctg ggggtgcatt ttgctgcgcc ggatacgctc cggcccaagc
     2641 ctgccgatcc cgggctggaa gtgattctgg tcaacgccag gcatgacaag gagccgctca
     2701 aggctgatgc tgtcgcgcag gccaatctgg acggcggtgg taatgccagt gaaggtcgcg
     2761 ccagttcgcc cttgcccgat atgcgcaagc tcaagagcgg cgacggcatc gaggcagcgc
     2821 agcgcacagt ggctgaactg gaggcgcggc aacagaaggt tctggcgcag atgaaccagc
     2881 tcacgccgac cgtcgccgtg ccggaacgtg agaagaccag gaaagtcccg cggcaatcca
     2941 gtgatcgcga tctgagcgac gccgccaggg agatggcgcg cttggaggcg gaagtggcca
     3001 ggaatatcga ggactacaac aggcggccga aaaagacgca gatcactgcg cgtacccgta
     3061 aagtcgacta tgccttttat tacaagcgca tgcaggaaaa gatagagata cttggcacgc
     3121 tgaacttccc gcacagggac ggcaggaagc tgtacggcga actgctggtg tatatcccgg
     3181 ttttccagga cggcacgatt tacgaaaagg aaggtggtgc gcgtgtcgaa cgcagttccg
     3241 gcaatgccgc gctcgacagc gcggcgctgg cgatcgtgca gcgctcggcg ccgttcggcc
     3301 gctttcccaa aaatatgcgc accacagaca aggacgatgt ctgggaaatc atcacccgtt
     3361 tccgcttcac gcatgacgag aacttgcgga tagacgcgga cagttcccac aattgaaaca
     3421 gcggagagtc agcatggatg catacgccgt tatcggtaat ccgatcgcgc acagcaaatc
     3481 gccggaaatt catgcgcgat tcgccgcgca gaccgggcag gagttgagtt atgagtgctt
     3541 gccggcacct ttgtcaggct tcagggcggt ggtccagagc ttcatcgccg atggtggcaa
     3601 gggagcgaac gtcactttgc ctttcaagct ggaagcgtat ggcctggccg gccggctgac
     3661 cgagcgcgcg cgcctggcgg gcgcggtcaa tacgctgaaa tttgaagatg gctgtattct
     3721 tggcgataac accgacggcg ccggtctggt gcgggatatc gtccggaatg ccggcgttgc
     3781 gctgagcggc aaacgggtct tgctactggg cgctggcggc gcggcgcgcg gtgccttgct
     3841 gcctttgctg gagcaacatc cgtccgaact ggtgctgagc aaccgcacac acaccagggc
     3901 gcgggaactg gcgacgcaat tcgccaccta tggcaatctg acggcgaccg agcatgccgg
     3961 cctgcgcggc gccttcgatg tcgtcatcaa tgccacttca gccagccttg cagccgacgt
     4021 accgccgctg ccggccaagg tttttttgcc gacgacgctg gcttacgaca tgatgtatgg
     4081 caagcagcca acggtgttca tggaattcgc ccggcggcac ggcgcgattg cacgcgatgg
     4141 tctgggcatg ctgatcgagc aggcggccga atctttcttt ttgtggcgcg gtgtgcagcc
     4201 gcaaacggca gaggtgtttg ctgcgctgcg cgcgtaatgg cggcaagctt gaacgttcgc
     4261 tggatttttt tatcacagat atcgaaaaat gaagcttctg cgtcaatcat tcctgtggct
     4321 gatcgtcttg tcggcaagtc tgctgttgct gctgcaattg tattttttcg cgcagatctg
     4381 gtggtgggtc gatcacaatc cggcttcgac cagcttcatg tgccatcaac tgtcgttact
     4441 gcaggaaaaa aatccccatg cccgtttgca atttcaatgg gtgccttaca gccggatttc
     4501 caaccacctc aagcgcgcca tcatcgcgtc ggaagattcc cgtttttccg ggcatgaggg
     4561 cgtggattgg gaggcgttac agcaagccta cgaaaagaac gtccgcaagg gtagggtagt
     4621 tgccggcggt tctacgatta cccagcagtt ggcgaagaat cttttcctct ccggagagcg
     4681 cagttatgcg cgcaaggcgc aggaattcgt gatcgcctat atgctcgaat ttctgatgga
     4741 taagcaacgc atctttgaga tttatctcaa tgtggtggaa tggggtaacg gcgtctttgg
     4801 tgccgaggcg gcggcgcggc attactatgg cagtagtgcc gccagtctgg gaccgagcca
     4861 ggcagcacgg ctggcggtga tgctgccgcg cccgcgttat tatgacagga atcctgactc
     4921 tgcctatctg gccagacgca ccgctttgat cctgcgccgt atgggtgcgg ccgagctgcc
     4981 tgcgtcgccg gccaggtctt cgacaaacaa taaactgcgc ctggagtctg catgatcaat
     5041 gttttcgttt tacagaatgg ccgactgaat caggtcaata tcgacagccg cagcgatctg
     5101 gaaaatgttg cgccggtctg ggtcgatctg accgagccga ccgatgacga gcgcgcttgg
     5161 gtcaagagca tttatggcgt ggctttgccg gacgaggatg aattcagcga tattgaagcc
     5221 tcggcgcgtt atttcgaagc ggagaacggc gatctgcatt tgcgcaccga ttttttgttc
     5281 gagagcgacg atactgcttc ggccaccatg acggtggcct tcatcttggc gcgcaacatc
     5341 ctgttttcca tgcatgcgga agatttgccg gtgtttcgct tggtgcgcat gcgcgcccgc
     5401 tcgcggccag gctcgatcgg cgattaccgc gatgtgctgc tcaatctcta tgaaaccgat
     5461 gccgaatatt ccgccgatgc gctcgaaggc atttatcaaa ggctagaaat cgtcagccat
     5521 agcgtactca agaaaagtct ctccgatcag gctgccgctg ccgtgctcaa taccatcgcc
     5581 catgaagaag acttgaacgg ccgcgttcgc cgcaacatga tggatactcg gcgcgcggtc
     5641 agtttcctga tgcgcggtcg cctgctgaat accgagcagt tcgaagatgc gcgccagatt
     5701 ctgcgcgaca tcgaatcgct ggatggccat actgccttcc tgttcgacaa gatcaacttt
     5761 ttgatggatg ccacggtcgg tttcatcaac atcaaccaga acaagatcat caagatcttc
     5821 tcggtcg
//
LOCUS       DASRDH010000049         2667 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_794949,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000049 DASRDH010000000
VERSION     DASRDH010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2667
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_794949"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="VFA14_01220"
     CDS             complement(<1..310)
                     /locus_tag="VFA14_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013233738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HZS80044.1"
                     /translation="MKDTLHKFMLESAPVRGELVEMSVTWQQVLARRDYPVAVRTLLG
                     EMLSAAALLSANLKFNGMIVMQIHGDGPVKLLVVECDAELRLRATARIVEDAVIADDA
                     T"
     gene            complement(371..895)
                     /locus_tag="VFA14_01225"
     CDS             complement(371..895)
                     /locus_tag="VFA14_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006712877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HZS80045.1"
                     /translation="MAIYKLGQDAPEIAETAYVAESAVVIGKVKLEEHTSVWCHASIR
                     GDNELIAIGENSNVQEGAVLHTDIGYPMRIGRNVAVGHQAMLHGCTIGEGVLIGIQAI
                     VLNGARIGRNSLVGAGALVTEDQEFPENSLIIGSPAKLARNLSPEDIARMRAGADSYV
                     ERAQLFKTSLKRIA"
     gene            1133..2143
                     /locus_tag="VFA14_01230"
     CDS             1133..2143
                     /locus_tag="VFA14_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007836077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HZS80046.1"
                     /translation="MMTLSIAVWHARGRKLFPGVFACAAIAAAATFLSRHYGVPVMLF
                     ALILGMAMNFLSGKGSCQPGIEFAARNVLRWGVALLGLRITTAQVVALGWESVFIVAI
                     SLVLTIAMSMVVARLMGFHTLFGLLSGGATAICGASAALALSAALPPHPLKERATLFT
                     VVSVSALSTFTMILHPMLARALGLDDVTAGIFLGATIHDVAQVVGAAYGMSQPVGDTA
                     TLVKLMRVACLLPVILFAVMYTRATGEATGEARPPLLPWFAVGFAVLMVINSTGWLPT
                     AVSKVGSDLSGWFLVASMVGIGMKTRLRELVAVGLKPVLLMLGETAFLAVLALALLRW
                     VS"
     gene            2273..2656
                     /locus_tag="VFA14_01235"
     CDS             2273..2656
                     /locus_tag="VFA14_01235"
                     /inference="COORDINATES: protein motif:HMM:NF012755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease domain-containing protein"
                     /protein_id="HZS80047.1"
                     /translation="MKIASLKQWTMFFAALLLSGTVFAGDAPGIDDIAVSSLPREARQ
                     TLNLIKRGGPYPYAKDGVVFGNYEGVLPRRKRGYYHEFTVNMPRAGNRGARRIIAAGN
                     PQTSGEYLLHQGSLPDIPAHQGMKL"
BASE COUNT          473 a          773 c          796 g          625 t
ORIGIN      
        1 gcgtggcatc gtcggcgatg acggcgtctt cgacgatcct ggcggtggcg cgcaggcgta
       61 gctcggcgtc acattcgacc accagcagct tgaccggacc atcgccgtga atctgcatga
      121 cgatcatgcc attgaatttg agattggccg acagcagggc tgccgcagat agcatttcgc
      181 ccagcagtgt cctcaccgcc accggatagt cgcggcgcgc cagtacctgt tgccaggtca
      241 cggacatctc caccagttcg ccacgcactg gcgcgctttc aagcatgaat ttgtgcagcg
      301 tatctttcat cgtttctttc tgatacgtat atattggata cgtacaaagt atctggtttt
      361 tgatagcgcc tcaggcgatg cgtttgaggc tggttttgaa caattgcgcg cgttcgacat
      421 aactgtcggc gccggcgcgc atgcgggcga tatcttccgg tgacaggttg cgcgctagct
      481 tggcggggga accgatgatc agcgaattct cggggaattc ctggtcttcg gtgaccagcg
      541 cgccggcgcc gaccaggcta ttcctgccga tcctggcgcc attgagtaca atcgcctgga
      601 taccgatcag gacaccttcg ccgatggtgc agccgtgcag catggcctgg tggccaacgg
      661 cgacgttcct gccgatgcgc atcggatagc cgatgtctgt atgcagcacg gcgccttcct
      721 ggacattgct gttttcgccg atggcgatca attcgttgtc gccgcggatg ctggcatggc
      781 accagacgct cgtatgctct tcgagtttga ccttgccgat cacgactgct gactcggcga
      841 cgtaggcggt ttctgcgatt tccggcgcgt cttgtcccag cttgtaaatt gccataatga
      901 agatgtcttc ccggtacagt cgagcatgca gattcccatg ttgaaatggg ctgtgttcca
      961 tcgccccgtc attttaccgc tgcttgcatg aattcatctt tgcgcgcgat ttcgcaaccc
     1021 aggatcaaga agatacttga caacatccct gttatctgta aaaacttttc ccggtttatg
     1081 tcttctcgtc cactgggtat gcgtaacatc attcacgggt tctgatgcga tcatgatgac
     1141 cctgtccatc gccgtctggc atgcgcgcgg gcgcaagctg tttccgggcg tgttcgcttg
     1201 cgcggcgatc gccgcagcgg ccacctttct gtctcggcac tacggtgtgc cggtgatgct
     1261 gtttgcgctg atactgggca tggcgatgaa ttttctctct ggtaaaggct cttgccaacc
     1321 cggcatcgag tttgctgcgc gcaacgtgct gcgctggggc gtggcgctgc tggggctgcg
     1381 cattacgacc gcgcaggtag tggcgctggg ctgggaatcg gtgttcatcg tcgccatctc
     1441 gctggtactg actatcgcca tgtcgatggt ggtggccaga ctcatgggct tccacaccct
     1501 gttcggcctg ctcagcggcg gcgccactgc catctgcggc gcctcggccg cgctggcact
     1561 gtcggcggcg ctgccgccgc acccactcaa ggagcgcgcc acgctgttta ccgtggtcag
     1621 cgtgtcggcg ctatccacct tcacgatgat cctgcacccc atgttggcgc gcgcgctggg
     1681 gctggacgac gtcactgccg gcatctttct gggcgccacc atccatgacg tggcgcaggt
     1741 ggtgggtgcg gcttacggca tgtcgcagcc agtgggcgat accgccacgc tggtcaaact
     1801 gatgcgggta gcctgcctgc tgccggtgat cctgttcgcc gtgatgtaca cccgcgccac
     1861 cggcgaggct acgggcgagg cgcgtccgcc gctactgccg tggtttgccg tcggctttgc
     1921 tgtgctgatg gtgatcaata gcaccggctg gctgcccact gccgtgagca aggtcggcag
     1981 cgatctgtcg ggctggttcc tggtggcgtc gatggtcggt atcggcatga agacccggtt
     2041 acgcgagctg gtcgccgtgg gtctgaagcc cgtgctgctg atgctgggcg agaccgcctt
     2101 cctggccgtc ctggcactgg cattattacg ctgggtgtcg tgaatcgatg ccctcgcctg
     2161 gaaggggcaa acatgcgagt tgcctggctt gaagcaaaac gttaccctac gcagtgatat
     2221 tgatattgcg aaaaattact atttacgcaa cataaacatg cgagcttgct tattgaaaat
     2281 agcgtccctg aaacagtgga ctatgttttt tgccgcgctg ctgctatctg gcactgtatt
     2341 tgccggagac gcgccgggca tcgatgacat cgcagtatcc agtctgcctc gggaagcgcg
     2401 tcagacactg aacctgatca agcgaggcgg accttatccc tacgcgaagg atggcgtggt
     2461 tttcggcaat tacgaaggtg ttttacccag gcgaaaacgc ggctattatc acgaattcac
     2521 cgtaaacatg cccagagcag gcaatcgcgg cgcccggcgc atcatcgccg caggcaatcc
     2581 gcaaacatcg ggtgagtact tattacacca gggatcatta ccagacattc cagcgcatca
     2641 gggaatgaag ctgtaagaac ctgttca
//
LOCUS       DASRDH010000050         2578 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1691211,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000050 DASRDH010000000
VERSION     DASRDH010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2578
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1691211"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..161)
                     /locus_tag="VFA14_01240"
     CDS             complement(<1..161)
                     /locus_tag="VFA14_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008329864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HZS80048.1"
                     /translation="MRYLITSIIIAVALWLLSSSIFIVEQRQSAIVFALGEVKQVVSE
                     PGLHFKLPPP"
     gene            complement(161..1462)
                     /gene="hflK"
                     /locus_tag="VFA14_01245"
     CDS             complement(161..1462)
                     /gene="hflK"
                     /locus_tag="VFA14_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HZS80049.1"
                     /translation="MLVSLFKKLGLKFSLNDPRWGHGSQDDDNRQQQNDHGNGNKRPE
                     NGPPDLDQLWRDFNQRLNRFLSKRGGNGAGDNNDNNFNHGDTKGAGIGVSVIVVIVAF
                     LWLASGFFIVQEGQTAVVMTFGKYSHTTLPGFNWRWPYPVQDHEIVNVSQVRTAEIGY
                     RSNAKNKQLKESLMLTDDENIIDIQFAVQYKLKNAAEWLFNNRDQDEAVRQVAETAIR
                     EIVGRSKMDFVLYEGREKVALDVNQLMQKILDRYESGVQITNVTMQGVQPPEQVQAAF
                     DDAVKAGQDRERQKNEGQAYANDVIPKASGAASRLLEEAEAYRSRIVANAEGDAARFK
                     QVLNEYQKAPAVTRDRMYLETMQQIFSNTTKVMVDARSGNNLLYLPLDKLIQQTDSAN
                     AIKPAMAVTAPTFPGVDPVDSTDVQRDARNRGVPDSEREVR"
     gene            complement(1495..>2578)
                     /gene="hflX"
                     /locus_tag="VFA14_01250"
     CDS             complement(1495..>2578)
                     /gene="hflX"
                     /locus_tag="VFA14_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007877288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HZS80050.1"
                     /translation="LLAKSAGAEPVTTITGKRASPDAALFVGSGKAQEIADAVADLRL
                     DVVIFGHALSPAQQRNLERLLKTRVLDRTSLILDIFAQRAKSHEGKVQVELAQLQHLS
                     TRLIRGWTHLERQKGGIGLRGPGETQLETDRRLLGQRVKVLRALLEKLRRQHATQRRA
                     RGRNDTFSVSLVGYTNAGKSTVFNAMAKASVYAANQLFATLDTTSRRVYLGEAGHVVI
                     SDTVGFIRDLPHQLVAAFRATLEETIHADLLLHVVDAASPLRMEQIEQVNLVLKEIGA
                     DHIPQILVWNKIDAAELGPSIEYDEYGKIRRVFISARTNIGLDFLRQAIADSLRSSIE
                     ARRGAFPVIDTQMVTHITRKLAGIPC"
BASE COUNT          554 a          714 c          706 g          604 t
ORIGIN      
        1 ggtggcggca gtttgaaatg caggcccggc tcgctcacta cctgcttgac ttcaccgagg
       61 gcaaacacga tcgctgactg gcgctgttcg acgatgaaga tactcgacga cagcaaccag
      121 agtgcgacgg cgataataat actggtgatc aaataacgca tcaacgtacc tcccgttcgg
      181 aatcgggtac accccggttg cgggcatcgc gctgaacatc ggtggaatcc actggatcga
      241 ctcctggaaa tgttggtgcc gttactgcca tcgccggttt aatggcattg gcgctgtcgg
      301 tttgctgaat cagtttatcc aatggcaggt atagcagatt gttgccgctc ctggcgtcca
      361 ccatgacttt ggtcgtgttg gagaaaatct gttgcatagt ttctagatac atgcgatcac
      421 gtgtcacggc cggcgctttt tgatattcgt tcagcacctg tttgaagcgt gctgcatcgc
      481 cttccgcgtt ggcgacaatg cgcgaacgat aggcttcagc ctcttccagc agacgcgaag
      541 cagcaccgct ggctttggga atcacatcgt tggcataagc ctgcccttca ttcttttgac
      601 gctcacggtc ctgtcctgcc ttgacagcgt catcgaaagc ggcctgtacc tgctcgggcg
      661 gttggacacc ctgcatggtg acgttggtga tttgcacacc ggactcgtag cgatcgagaa
      721 ttttctgcat cagctggttg acgtcgagcg ccaccttttc gcgaccttca tacaggacga
      781 agtccatttt gctgcgaccg acgatctcac gaatcgcggt ttcggcgacc tggcgcacgg
      841 cttcatcctg atcacggtta ttgaataacc attctgccgc gttcttgagc ttgtactgga
      901 ctgcgaactg gatgtcgatg atattttcat catcggtcag catcagggat tctttgagct
      961 gcttgttctt ggcgttgctg cgatagccga tttcggccgt gcgcacttgc gagacattga
     1021 caatctcatg atcctgcacc ggatacggcc agcgccagtt gaaacccggc agcgtggtat
     1081 gactgtattt accgaaggtc atgacgacgg cggtctggcc ttcctgcaca atgaaaaagc
     1141 cgctggcaag ccacaagaaa gcgacgatca ccacgattac actgacaccg ataccggcac
     1201 ctttcgtgtc accgtgattg aaattgttat cattattatc gccagcacca ttgccgccgc
     1261 gcttgctcaa aaaacggttc agacgctggt tgaaatcgcg ccagagctga tcgagatcag
     1321 gaggtccgtt ttccggcctt ttattgccgt tgccgtggtc attttgttgt tgacggttgt
     1381 cgtcatcctg cgaaccatgc ccccagcgag gatcgttcag cgagaatttc agaccgagtt
     1441 ttttgaatag agaaacaagc atgcgtgcga atccggctta attatcaaat tgaatcaaca
     1501 gggaatgccc gccaacttcc gggtgatatg cgtgaccatc tgcgtatcga taaccggaaa
     1561 tgctccccgg cgcgcctcga tcgacgatct gagtgaatcg gcaatcgcct gccgcaaaaa
     1621 atccaggccg atattagttc tcgcactgat aaaaacccga cggattttac catactcatc
     1681 gtattcgatt gatggtccca gctcggcggc atcgatcttg ttccagacca gaatctgcgg
     1741 gatatgatcc gcgccgattt ccttgagtac gaggttgacc tgttcgatct gttccatgcg
     1801 gagcggactt gcggcatcga cgacgtgcag cagcaaatcg gcgtgaatgg tttcttccag
     1861 ggtcgcgcga aacgctgcca ccagctggtg cggcaagtcg cgaatgaagc cgaccgtatc
     1921 ggaaatgacg acgtgtccgg cttcgcccag atagacacga cgcgaggtgg tgtccagcgt
     1981 tgcaaacagc tggttcgctg cgtacacgct ggccttggcc atagcgttga acaccgtcga
     2041 tttgccagca ttggtataac cgaccagaga aacggaaaac gtgtcattgc gcccgcgcgc
     2101 gcgccgctgc gtggcatgct gacggcgcag cttttccaga agagcgcgca ataccttgac
     2161 acgttgacca agcaggcgac ggtcggtttc tagctgcgtt tcaccgggac cgcgcaggcc
     2221 gataccaccc ttttgccgtt ccaggtgagt ccagccacga atcaatcgcg tcgacaaatg
     2281 ttgcagttgc gccagttcga cctgcacctt gccttcgtgg cttttggcgc gctgggcaaa
     2341 gatatccaga atcagactgg tgcgatcgag tacccgcgtt ttcagcagac gctcaagatt
     2401 acgctgttgc gcaggagaca gggcgtgacc gaagatgacg acatcaagtc gcagatctgc
     2461 gaccgcgtca gcaatttcct gagccttgcc ggaaccgaca aacaaagcgg catccggact
     2521 ggcgcgcttg cccgtaatgg tcgttacagg ctcggcgcca gcggacttcg cgagcagg
//
LOCUS       DASRDH010000051         2178 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1468196,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000051 DASRDH010000000
VERSION     DASRDH010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1468196"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            174..473
                     /locus_tag="VFA14_01255"
     CDS             174..473
                     /locus_tag="VFA14_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS80051.1"
                     /translation="MTTTPSGNPQWRAANGAVIYGYQMIPLPFTARPHTPPSTGTYRD
                     WVRRMGTNRTRGIGGWIIKLPTDPGATWFVTTADDNTDTAAGTTNDALHPPAGVK"
     gene            complement(484..1185)
                     /locus_tag="VFA14_01260"
     CDS             complement(484..1185)
                     /locus_tag="VFA14_01260"
                     /inference="COORDINATES: protein motif:HMM:NF014941.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HZS80052.1"
                     /translation="MTDDEKRIARRAYNRAWREANKVSQAARQRTRIAAKTAEERAKD
                     AAYQRAYRAANLEQLKAKGLAWREKNAEHIKEKRKAYKATRRQEISAKQKAYHQATKE
                     IRKEALAANALKQNRKRPALLLARQEAALNRKKPDICDACGGNDGGIVFDHCHQAMFP
                     RGWLCDRCNVALGCLRDSPERLRQLVAYLERNETGITPEFMIAGRFASRYRTAGSRRH
                     VVVEDDTGHSLTRIG"
     gene            1419..>2178
                     /locus_tag="VFA14_01265"
     CDS             1419..>2178
                     /locus_tag="VFA14_01265"
                     /inference="COORDINATES: protein motif:HMM:NF019126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HZS80053.1"
                     /translation="MPIGSVLDFAGAQAPQGWLICDGRSISRTTYSDLFAVLGTVWGA
                     GDGSTTFNLPNPNGRSSVGPGTVTDAAGRTRAFAFAQQNLGYVWNVIGQAHLPNYALG
                     IDYQGYHSHGGATTNAGAHYHTTDGQGSHSHGGATGGDGNHQHGTDTQGAHQHFVTAA
                     YMNAGGNAFSGSAQTIQNTGLYTDVQGAHSHTTTTAGFHYHTLSADGYHAHTTSWVGD
                     HGHGINGDGSHIHNVYLNGSGTPFEVMAPVIVMTK"
BASE COUNT          409 a          685 c          644 g          440 t
ORIGIN      
        1 cacggcgact gtgctgcgtc gtatcgacag cgcgtatata ccggacgatc ccgccaaccg
       61 ggacagagcc gaatacaatt catggctggc cgatggcggg gtgcccgatc cagcgccgac
      121 gccgcaggcg cccgcagcta aggaggaatg acatggctac aacagctggc ctgatgacaa
      181 caactccaag cggtaaccct cagtggcgcg ctgccaatgg cgccgtcatc tacggctacc
      241 agatgatccc gctgccgttt actgcacggc cacacacgcc gccgtctacc ggtacttata
      301 gggactgggt gcggcgcatg ggcaccaacc gtacgcgcgg catcgggggc tggatcataa
      361 aactgccgac agatccaggt gccacttggt tcgtcacgac agcagatgac aacaccgaca
      421 cggctgccgg gaccaccaac gatgcgctcc atccgcccgc tggcgtgaag tgacatgccc
      481 tgtctacccg atccgggtaa gagaatgacc ggtgtcgtct tctaccacca catgacgacg
      541 cgatcccgct gtgcgatagc gactagcgaa ccgtccagcg atcatgaact cgggggtaat
      601 cccggtttcg ttgcgttcaa ggtaggcgac cagttgacgg agccgctcgg ggctgtccct
      661 gaggcatccg agcgcgacgt tgcagcggtc gcagagccag cctcggggga acatcgcctg
      721 gtggcagtgg tcgaagacaa tgccaccgtc attgccgccg caagcatcgc agatgtcggg
      781 tttcttgcgg ttaagcgcgg cctcttgtct ggcgagaaga agcgcaggtc gtttacggtt
      841 ctgtttcagg gcgtttgcgg ccagcgcttc tttccgaatt tccttcgtgg cctggtggta
      901 ggctttctgt ttggcgctga tttcctgtct tctggtcgcc ttgtatgcct tgcgcttctc
      961 ttttatgtgc tccgcattct tctcgcgcca ggccaggccc ttcgccttga gttgttcgag
     1021 attggccgca cggtatgcac gttgataagc cgcatccttg gctctctcct cagcggtttt
     1081 ggcggctatt cttgtccgtt gtcgcgctgc ttgactaacc ttgttagcct cgcgccaggc
     1141 gcggttatac gcgcgccgcg caatgcgctt ttcatcatca gtcatgttag gcttcatcag
     1201 cttcgatcct tggttacagc aggggtcggg gtcagggatg gtggctatgt atcgagcatc
     1261 gccaccatcc cgcttatagc taaagggggc tggtaatgcc agatagtttt acttcgtccc
     1321 tcgggctctg tcagccggaa gtgggcgcat ctcgcgattc gtggggcgca aagtggaacc
     1381 agaacgcaac aatcctcgat gagttcgttt ccgttgccat gccgatcggc agtgtcttgg
     1441 acttcgcagg cgcgcaggcg ccgcaaggtt ggctaatctg cgatggccgt tcgatctcgc
     1501 gcaccaccta cagcgacctg ttcgcggtct tgggcaccgt ctggggcgct ggcgacggct
     1561 ccacaacttt taacctaccc aacccgaatg gccgctcgtc ggttggtcca ggcaccgtca
     1621 ccgacgctgc ggggcgcacg cgggcctttg ccttcgccca gcagaacctc ggttacgtct
     1681 ggaacgtcat cgggcaggct cacctgccga actacgcgct cggcattgat taccagggat
     1741 accactcgca cggcggcgct acgaccaacg caggcgcgca ctatcacacc accgatgggc
     1801 aggggtcaca cagccacggc ggagccacag gcggcgacgg caatcaccag cacggcaccg
     1861 acacgcaagg ggcgcatcag catttcgtca ctgccgctta catgaacgca ggcgggaatg
     1921 cttttagcgg cagcgcacag accattcaga acaccggcct ttacaccgac gtgcaggggg
     1981 cgcactccca caccacgaca actgccgggt tccactatca cacgctatcg gctgacggtt
     2041 atcatgccca cacgaccagc tgggtcggcg accacgggca cggcatcaac ggtgacggca
     2101 gccacattca taacgtctac ctgaacggca gcggcacgcc gttcgaggtg atggcgccgg
     2161 tcatcgtcat gaccaaga
//
LOCUS       DASRDH010000052         8010 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_792323,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000052 DASRDH010000000
VERSION     DASRDH010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 8010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8010
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_792323"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..799)
                     /locus_tag="VFA14_01270"
     CDS             complement(<1..799)
                     /locus_tag="VFA14_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS80054.1"
                     /translation="MTFNQRTVALLQLPVSSITSDGILKECDRLEAAFPHISSPFKWT
                     LDYKRFQVEHARGNHGTALAHLKAAIAITPYNDELIGDYRKLVEKTVGIGSLVVVLTS
                     KAGEARALRLAGQLDQTDVQYAIVSGDDTPSIAHVRAVQVAASDCHEAVPRKAVAAFA
                     WVRENIGSNVGVLKVSDDVVLEDGRQLAASLQRLAVEGGYAGSPVGGLEHDRCLHWGL
                     CLNQELNRRVYGRPMLRPWASGAAYYLGPRALDKVVLALMRFPGLFEG"
     gene            889..1026
                     /locus_tag="VFA14_01275"
     CDS             889..1026
                     /locus_tag="VFA14_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS80055.1"
                     /translation="MQHKQAAILTKEQEHAEFTLNFAFGQVTQDARKALRVTLSPYFP
                     A"
     gene            1029..2387
                     /gene="glmU"
                     /locus_tag="VFA14_01280"
     CDS             1029..2387
                     /gene="glmU"
                     /locus_tag="VFA14_01280"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HZS80056.1"
                     /translation="MNVVILAAGIGKRMHSALPKVLHPLAGRPLLEHVVDTAKTLAPS
                     KLCVVYGHGGAAVPSRFTDSTLAFVKQEPQLGTGHALAQALPLLDDSAPTLVLYGDVP
                     LITAATLQRLLADASNDKLAVLTVTLDDPAGYGRIVREHDKIIRIVEQKDAGAAERAI
                     REVNTGIIAVPTVRLRAWLAALSNDNAQGEYYLTDIIASAIADGVDVVSAQPADAAEI
                     LGVNSKAQLAKLERVYQRRIADALLEQGVTLLDPARLDVRGVLTCGRDVSIDVNCIFA
                     GTVVIEDGAIIGANCIIQNTRIGRNARIEPFSHIDDAIIGAAAQVGPYARLRPGTELG
                     EAVHIGNFVEVKKSHIAARSKASHLAYLGDARIGADVNIGAGVITCNYDGVNKSETII
                     EDDVFVGSDTQLIAPVRVGKGASIGAGTTLTRDAPAGQLTLSRAKQVSLAGWKRPVKI
                     SK"
     gene            2414..4231
                     /gene="glmS"
                     /locus_tag="VFA14_01285"
     CDS             2414..4231
                     /gene="glmS"
                     /locus_tag="VFA14_01285"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HZS80057.1"
                     /translation="MCGIVGAVAQKNVTPILLEGLQRLEYRGYDSCGVAVYMDGKLQR
                     SRSTARVAELRKQIAATHLAGCTGIAHTRWATHGAPATHNAHPHFSRERIALVHNGII
                     ENYEELHAELQSAGYHCESQTDTEVIAHLIDHMYTGDLFETVQQAVRRLTGAYAIAVF
                     CVDEPDRVVAARRGSPLIVGIDADQHFVASDAMALAGVTERIIYLEEGDVVDLQRQCY
                     RIVAANGASVERQVNTVCAFTGAAELGPYQHFMQKEIFEQPRAIADTLEGIDSIMPEL
                     FGDDAHQIFKRIDSVLILACGTSYYAGLTARYWLEAIARIPVSVEIASEYRYRDSVAN
                     PATLVVTISQSGETADTLAALKHARLQGMASTLTICNVAASAMVRESQLAYITRAGVE
                     VGVASTKAFTTQLAALFLLTLTLAHTRGQLSDEAATAHLKAMRHLPAALQSVLALEPG
                     IVAWAETFARKENILFLGRGLHYPIALEGALKLKEISYIHAEAYAAGELKHGPLALVT
                     EAMPVVTIAPNDTLIEKLKSNMQEVRARGGQLYVFADAGSSITTSEGLHVIRLPEHYG
                     LLSPILHVVPLQLLAYHTALARGTDVDKPRNLAKSVTVE"
     gene            4414..5376
                     /locus_tag="VFA14_01290"
     CDS             4414..5376
                     /locus_tag="VFA14_01290"
                     /inference="COORDINATES: protein motif:HMM:NF023776.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3644 domain-containing protein"
                     /protein_id="HZS80058.1"
                     /translation="MFDKAVSAMIAAIEIYNKPTFEYREETFAVLAINAWELLVKAKH
                     LKENGNKLSTLYIPIKAKTASGASSKRAKYKQNRTGNPMTHSLEELAEKLMAQKHLDT
                     EALQNLKALLVIRDEAIHFYNTNPLLSQRLQEIGTATVRNFIIASTEWFQADFSTYNF
                     HLMPLAFVNPNDAADAIILSKEEQRIASFIESLDNGIESDRKYVVSVNVQMRFVRSSV
                     KDAIQVQLSNHPDATPIRLTDAQFDEKYKMNYEELCQACKRRYSNFSANQKFHDIKKP
                     LQENQKYALVRELDPGNPKTVKKTFYSEAIFSELDKHYEKNRGN"
     gene            complement(5452..5853)
                     /locus_tag="VFA14_01295"
     CDS             complement(5452..5853)
                     /locus_tag="VFA14_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012212496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HZS80059.1"
                     /translation="MPLFMLDTDMCIYLMKNQPEQVAKRFAQCYVGDVVMSAITFAEL
                     EYGVTVSAHSVRERRNLVALVEDIPVAAFDAFAASAYGPIREATRECRKDQLDKLIAA
                     HAVALDVTLVTSNERDFVAYPGLRIENWIDG"
     gene            complement(5853..6089)
                     /gene="vapB"
                     /locus_tag="VFA14_01300"
     CDS             complement(5853..6089)
                     /gene="vapB"
                     /locus_tag="VFA14_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006759705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HZS80060.1"
                     /translation="MRITKAFRNGNSQAIRIPSELTYERTDIELEIERVGEEIRIRPV
                     RRPLVGVLRKFAKFGPDFMADGRGDQEQAERKTL"
     gene            6378..6542
                     /locus_tag="VFA14_01305"
     CDS             6378..6542
                     /locus_tag="VFA14_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS80061.1"
                     /translation="MRDWTYKRSLRMQGIVELRAVWQEMLSDKTPGISANDVLNRLER
                     KYQTIANAGG"
     gene            6552..6845
                     /locus_tag="VFA14_01310"
     CDS             6552..6845
                     /locus_tag="VFA14_01310"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HZS80062.1"
                     /translation="MRIELSRYIEGDLDAIADFIAQDNPQRAVTFIQDIRERFHEVQC
                     EPLIYRLRPDIGEDARMATIGDYAVLFHITGNVVRIERVVYGGHNLPDVFDLS"
     gene            complement(6849..7637)
                     /gene="arsH"
                     /locus_tag="VFA14_01315"
     CDS             complement(6849..7637)
                     /gene="arsH"
                     /locus_tag="VFA14_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008328811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenical resistance protein ArsH"
                     /protein_id="HZS80063.1"
                     /translation="MMITANPQQDSFTKEGGEVVIDAQQHGALLPNIDPTLFQTPLVP
                     DFSKITTSTHRPRFLLLYGSVRERSYSRLLVMEAARLLEAMGGTVRIFDPHGLPLPDS
                     EPETHPKVAELRELASWSEGMVWCSPERHGAMSGILKAQIDWIPLSQGAIRPTQGKAL
                     AVMEVSGGSQSFNAVNQMRVLGRWMRMLTIPNQSSVAKAWQEFDDAGRMKPSAYYERV
                     VDVMEELFKFTLLTRDIAPFLTDRYSERRETAAELSKRMNQARL"
     gene            complement(7698..>8010)
                     /locus_tag="VFA14_01320"
     CDS             complement(7698..>8010)
                     /locus_tag="VFA14_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014250494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZS80064.1"
                     /translation="IFGPLLAVTSFTDVEEAIALANDTVYGLAAALYTSDLDTAIRLA
                     RRIRAGIVTVNCFGEGDITVPFGGYRQSGFGGRDKSMWAHHQYTEIKTIWIDVPDAEY
                     R"
BASE COUNT         1871 a         2442 c         2139 g         1558 t
ORIGIN      
        1 cgccttcgaa caaccccgga aaacgcatca gcgccagcac caccttgtcc agcgcgcgcg
       61 gccccaggta atacgctgca cccgaagccc agggccgtaa catcggacgg ccatacacgc
      121 gacgattcaa ctcctggttc aggcacagcc cccagtgcag acaacggtcg tgctccagac
      181 caccgacagg cgaaccggca tatccaccct cgacagccag gcgctgtaag ctggcggcaa
      241 gctgacgccc atcctcgagt acgacatcgt cgctgacttt cagcacaccc acattgctgc
      301 cgatgttttc gcggacccag gcaaaagccg ccaccgcctt gcgcggcacg gcttcatggc
      361 agtcagaagc agccacctgc actgcccgga cgtgggcaat cgacggcgta tcgtcgccgc
      421 tgacgatcgc atactggaca tccgtctggt cgagctggcc cgccagacgc aaggccctgg
      481 cctcgccggc cttgctggta agcacgacca ccaggctgcc gattccgaca gtcttttcca
      541 ccagtttcct gtagtcaccg atgagttcgt cgttgtaagg cgtgatggcg atagcggcct
      601 tcagatgggc gagcgccgtg ccgtggttgc cacgggcatg ctcgacctgg aaacgcttat
      661 agtccaaagt ccatttgaac ggggaggaaa tgtgcgggaa tgccgcctcc agccggtcgc
      721 attccttgag aatgccgtcg gaagtaatgg aagaaaccgg caactgcaac agcgctacgg
      781 tacgttgatt gaatgtcatg atcagtccac catctggaat gctgataacc cgttttcctg
      841 atgccggccc gcgcacacga cagataaaga aagcagtccg aagcatggat gcagcataaa
      901 caagctgcca tcttaacaaa ggaacaagaa catgcggaat tcaccctcaa ctttgctttt
      961 ggacaagtca cacaagatgc gcgaaaagca ttgcgtgtta cactttcgcc ttattttcca
     1021 gcctgatcat gaacgttgtt atcctcgccg ccggcatcgg caagcgcatg cactcggcgc
     1081 tgccgaaagt cctgcatcca ctcgccggcc ggcctttgct ggagcatgtt gtcgataccg
     1141 ctaaaacgct cgcgccatcg aagctgtgcg tggtctacgg tcatggcggc gctgccgttc
     1201 cttcccgttt taccgacagc acgctggcat tcgtcaaaca ggaaccgcag ctcggcaccg
     1261 gccatgcgct cgcccaggca ctgcctttgc tcgatgacag cgcgccgacg ctggtgctgt
     1321 acggcgatgt tccgctgatc acggcagcaa cgctgcaacg cctgctggcc gacgccagca
     1381 atgacaaact ggcggtgctg acagtgacgc tggacgaccc cgccggctac ggccgcatcg
     1441 tgcgcgagca cgataaaatc atccgcatcg tcgagcaaaa agacgctggc gcggccgaac
     1501 gcgccattcg ggaagtcaac accggcatca tcgccgtccc taccgtccgg ctgcgcgcct
     1561 ggctggcagc gctgtccaac gacaacgccc agggcgagta ttacctgaca gatatcatcg
     1621 ccagcgcaat tgccgacggc gtcgacgtgg tgtcggcgca gccggccgat gccgccgaaa
     1681 ttctgggcgt caacagcaag gcgcaactcg caaaactcga gcgcgtgtat cagcgccgca
     1741 ttgccgatgc actactggaa cagggcgtca cgctgctcga tcctgcacgc ctggatgtgc
     1801 gcggtgtgct gacatgcggc cgcgatgtca gcatcgatgt caactgcatt tttgcaggca
     1861 cggtcgtcat cgaagacggc gcgatcatcg gcgccaactg catcattcaa aatacacgca
     1921 tcggcagaaa cgcgcgcatc gagccgttct cgcacatcga cgacgccatc atcggcgcag
     1981 cagcacaggt cggcccttac gcgcgtctgc gccccggcac cgagctgggc gaagcagtgc
     2041 atatcggcaa cttcgtggaa gtgaaaaaaa gccatatcgc tgcgcgcagc aaggccagtc
     2101 acctggccta cctcggtgac gccaggatcg gtgcggacgt caacattggc gccggcgtga
     2161 tcacctgcaa ttatgatggc gtcaacaagt ccgagaccat catcgaggac gacgtctttg
     2221 tcggcagcga cacccaactg atcgcgccgg tgcgcgtcgg caaaggcgcc agcatcggcg
     2281 ccggcaccac actgaccaga gacgcgccag ccggccagtt gacgctgtcg cgcgctaagc
     2341 aagtgtcgct ggcgggatgg aagcgtccgg tcaagatcag caaataagca acttaggtaa
     2401 ggaaagactc cccatgtgcg gtatcgttgg cgccgttgcg caaaaaaatg tcactccgat
     2461 tttgctggaa ggtttgcaac gcctcgaata ccgcggctat gattcctgcg gtgtggccgt
     2521 ctacatggat ggtaaactgc aacgctcgcg cagcacggcg cgcgttgccg aactgcgcaa
     2581 gcagatcgcc gcgacgcatc tggccggctg caccggcatt gcccacaccc gctgggccac
     2641 gcatggcgca ccggcgacgc acaacgccca cccgcatttc tcgcgcgagc gcatcgcgct
     2701 ggtccacaac ggcatcatcg aaaattacga agaactgcac gccgaactgc aaagcgccgg
     2761 ctaccactgt gaaagccaga ccgacaccga agtcatcgcg cacctgatcg accacatgta
     2821 caccggcgat ctgttcgaga ccgtgcagca agcggtgcgc cgtctgacgg gcgcctacgc
     2881 catcgccgta ttttgcgtcg atgaacccga tcgcgtggtc gccgcgcgtc gaggctcgcc
     2941 attgatcgtc ggcattgacg cagaccagca tttcgtcgct tccgacgcca tggcgctggc
     3001 tggcgtgacc gagcggatca tttatctgga agaaggcgac gtcgtcgatc tgcaacggca
     3061 atgctaccgg atcgtcgccg ccaacggcgc atccgtcgag cgccaagtca acaccgtgtg
     3121 cgccttcacc ggcgcggccg aactcggccc ctaccagcac ttcatgcaaa aggaaatttt
     3181 cgaacagccg cgcgccatcg ccgacacgct cgaaggcatc gacagcatca tgccggaact
     3241 gttcggcgac gacgctcacc agatcttcaa gcgcatcgac tccgtactga tcctggcctg
     3301 cggcaccagc tattacgccg gccttaccgc caggtactgg ctcgaagcga tcgccaggat
     3361 tccggtcagc gtcgaaatcg ccagcgaata ccgctaccgc gacagcgtcg ccaatcccgc
     3421 aacgctggtc gtcaccatct cgcaaagcgg cgagaccgcc gacacgctgg ccgcgctcaa
     3481 acatgcacgc ctgcaaggca tggccagcac gctgaccatc tgcaacgtcg ccgccagcgc
     3541 catggtgcgc gaatcgcaac tggcctacat cacgcgcgcc ggcgtcgaag tcggcgtcgc
     3601 ctcgaccaag gcgttcacca cacaattggc ggccttgttt ttattgacgc tgacactcgc
     3661 ccacacccgc ggccagctca gcgatgaagc cgcaacggcg cacctcaaag ccatgcgcca
     3721 cctgcccgcg gcactgcaaa gcgtgctggc gctggagcct ggcatcgtcg cctgggccga
     3781 aaccttcgcc cgcaaggaaa atatcctctt cctcggccgt ggcctgcact acccgatcgc
     3841 gctcgaaggc gcactcaaac tcaaggaaat cagctacatc cacgccgaag cctacgccgc
     3901 cggcgaactc aagcacggcc cgctggcgct ggtcacggaa gccatgccgg tcgtcaccat
     3961 tgcgcccaac gacaccctga tcgaaaagct caagtccaac atgcaggaag tccgcgcccg
     4021 cggcggccag ctctacgtgt ttgccgacgc cggctccagc atcacaacca gcgagggcct
     4081 gcacgtcatc cgcctgccgg aacactatgg cctgctgtcg cccatcctgc acgtggtgcc
     4141 cttgcaattg ctggcctatc acaccgcgct ggcgcgcggc accgacgtcg acaagccgcg
     4201 taatctggca aaatcggtga cggtggagta gctatcgaca gataaaaatt tcgacacgaa
     4261 attgatgata ggctcgctga agctgaagca aactactata agcaactcag aaatgccgct
     4321 gccgaacctg cattaactta aagaggtaag cccccggctt tgccggggga ctcagtcagt
     4381 ttcaggataa atatgagagc acgctcacag cagatgttcg ataaggcggt ctccgctatg
     4441 attgcggcga tcgaaatcta caataagccc acatttgaat atcgagagga aacgttcgcc
     4501 gttcttgcca taaatgcttg ggagttgctt gtcaaagcga aacatctcaa ggagaacggg
     4561 aacaaacttt ccactctcta tatacccatc aaagcaaaga cagcctcagg ggcgtcctcc
     4621 aaacgagcta aatacaagca aaatcgcacg ggcaatccca tgacgcatag cctggaagaa
     4681 ttagccgaaa agctcatggc gcaaaagcat ttggacacag aagctttgca gaaccttaaa
     4741 gcattgcttg tcattcgaga cgaggcgata catttctata acaccaatcc gctgctttct
     4801 cagcgtctac aggaaattgg cacagcgacc gtccggaatt tcattattgc gagtaccgaa
     4861 tggtttcaag ctgatttctc tacctacaac ttccacttga tgccgctggc atttgtaaat
     4921 cccaatgacg cggctgacgc aatcatactt tccaaagaag aacagcgaat tgccagtttt
     4981 atcgagtctc tcgacaatgg aatagagagc gatcgcaagt atgttgtctc cgtcaatgtg
     5041 cagatgcgat ttgtacgctc gtcagtaaag gacgccattc aggtgcaact atctaatcat
     5101 cctgatgcaa ctccaatccg actcacggat gcacagttcg acgagaaata caaaatgaac
     5161 tacgaggagc tgtgccaagc ctgcaaacga cgatattcaa acttcagtgc aaatcaaaaa
     5221 tttcatgata taaaaaagcc gttgcaagaa aaccagaaat acgcgctagt acgagaactg
     5281 gacccaggaa atccgaaaac ggtaaagaag accttctatt cagaggcgat atttagcgaa
     5341 ctcgataaac actacgaaaa aaataggggg aattagatat gtcataaatt ttgtgtgccg
     5401 agatagttta atcatctaaa taacttggtc tctagcttca tgccagtttt cctatccatc
     5461 gatccaattt tcaattcgca gtcctgggta agcgacgaaa tcacgctcat tgcttgtgac
     5521 cagcgtcaca tcgagcgcca cggcatgcgc agcaatcagc ttgtcaagct gatcctttct
     5581 gcactcgcgc gtcgcctctc tgatcggccc ataggccgat gcagcaaaag catcgaatgc
     5641 agcaaccggg atatcttcga caagtgcaac aaggttgcgc cgttcacgca cactgtgcgc
     5701 agatacggtg acgccatact ctaattcggc aaaggtaatg gccgacatca ccacatcgcc
     5761 gacataacac tgagcaaagc gtttggcgac ctgttcaggc tgatttttca tcaggtaaat
     5821 acacatgtcg gtatcgagca taaacagcgg cattacagcg tcttccgttc tgcctgctcc
     5881 tgatcaccac gcccatcggc catgaagtca ggcccgaatt tggcaaattt cctgagaacg
     5941 ccaacaagcg gcctacgcac aggccggatg cgaatttcct cgccgacgcg ctcgatctcc
     6001 aactcgatat cggtacgctc ataggtaagc tcggatggga tacggatcgc ctgggaattg
     6061 ccattccgaa aagctttggt tatccgcatc tccctctcca caaatgtgtg tacacgggtg
     6121 tactgtaaac catatgcgga ttctgacgcc ggagtcctct caattcagcg ccggcaatgg
     6181 caaaccgatt acccgttcac cgattccttc agaattgcta tcatggtggg aatgattcat
     6241 accatccgca tgaaaggcat gtcgatgact accgccgaga aaatcagtat tgccctgccg
     6301 ccagaaatgg ttcatatcgt gcgtgccgct gttgcaaccg gcgaatacgc atcgagagtg
     6361 aagttattcg tgacgccttg cgagactgga catacaagcg cagcttgcgc atgcaaggca
     6421 tcgtcgaact tcgcgctgtc tggcaggaga tgttgagcga caaaacaccc ggcatttcgg
     6481 caaacgatgt attaaatcgc cttgagcgca agtaccagac catcgccaat gctggggggt
     6541 gaggcaaagt aatgcggata gagctttcgc gctatatcga gggtgatctg gatgcaatcg
     6601 ccgattttat cgcccaggat aatccgcagc gcgcagtcac attcatccag gacattcgcg
     6661 agaggtttca tgaggtccag tgcgaaccat tgatctaccg gctacgcccg gatattggcg
     6721 aagacgcgcg catggctacc attggggatt atgcagttct attccatatc acaggaaacg
     6781 tcgtgcggat cgagcgcgtt gtctacggtg gtcacaattt gccggatgtt ttcgatctgt
     6841 cataaaagct acaaacgcgc ctggttcata cgctttgaca gctcagcggc tgtctcgcgg
     6901 cgctcactat accgatcggt cagaaacggc gcaatgtccc tggtcagcag cgtgaacttg
     6961 aacagctcct ccatcacgtc aaccacgcgc tcgtagtacg ctgaaggctt catcctgccg
     7021 gcgtcgtcga attcctgcca ggctttagcc accgacgact gattggggat ggtcagcatc
     7081 cgcatccagc ggccaagcac gcgcatctgg ttgacagcat tgaaagactg cgagccgccg
     7141 gacacttcca tgacagccag cgcctttccc tgagtcggtc gaatcgcccc ttgcgacagt
     7201 ggaatccagt cgatctgggc tttcaggatg ccgctcatgg cgccatggcg ctccggggaa
     7261 caccacacca ttccttcgga ccagctggcc agttcgcgca gctcggcgac cttcggatgc
     7321 gtttcaggct cgctatccgg tagcggcaag ccgtgcggat cgaatatcct gactgtgcca
     7381 cccattgcct ccagcagccg ggctgcttcc atgacaagca ggcggctgta ggagcgttcg
     7441 cggacagaac cgtacagcaa cagaaaccgc ggcctgtgag tcgaagtagt gatctttgaa
     7501 aaatcgggaa ccaggggtgt ctgaaacaga gtcggatcga tgttcggcag tagtgcgcca
     7561 tgctgctgcg catcgatcac gacttcgccg ccttcctttg taaaagaatc ttgctgtgga
     7621 ttcgcggtta tcatcatgct ccctgcaatc agtgtaaatc gccgtggcga gtccggtaag
     7681 cgtcgcctgg ctgctgttca tcgatattcg gcgtccggca cgtcgatcca gatagttttg
     7741 atctccgtgt actggtggtg cgcccacatc gatttgtcgc ggccgccgaa gccggactgc
     7801 cggtagccgc cgaaaggcac ggtaatgtca ccttcgccga agcagtttac cgtaacgata
     7861 ccggcgcgga tacgacgcgc caggcggatc gccgtgtcga ggtcgctggt gtagagcgca
     7921 gccgccaggc cgtacaccgt atcgttggcc aatgcgatgg cttcttcgac gtcggtgaaa
     7981 ctggtcaccg caagtaacgg accgaaaatc
//
LOCUS       DASRDH010000053         2149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1344486,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000053 DASRDH010000000
VERSION     DASRDH010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1344486"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            49..402
                     /gene="fliE"
                     /locus_tag="VFA14_01325"
     CDS             49..402
                     /gene="fliE"
                     /locus_tag="VFA14_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013234046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HZS80065.1"
                     /translation="MAVGVIDTSRIEAMVAQLKAAAARGQGNVAPIENMSASEKQATR
                     VDFAGVLKNSLDEVNSLQQKAKKMSESFSMGNDDINLSDMMIAAQKASIAFQTSLQVR
                     NKLVSAYRDIMTMQV"
     gene            complement(566..1156)
                     /gene="orn"
                     /locus_tag="VFA14_01330"
     CDS             complement(566..1156)
                     /gene="orn"
                     /locus_tag="VFA14_01330"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007880297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HZS80066.1"
                     /translation="MSQATHRAAPAYAASVRPNELNLIWLDMEMTGLDPDRDRIIEVA
                     IVVTDPGLNVLAEGPVFAIHQSDAILDGMDAWNKSTHGRSGLIERVKTSTVTEAEAEE
                     ALIDFLKHFVPAGKSPMCGNSICQDRRFMVRGMPRLEAFFHYRNLDVSTLKELCRRWK
                     PELASGFKKHQKHTALADIIESIEELKYYREHFIKE"
     gene            1276..>2149
                     /locus_tag="VFA14_01335"
     CDS             1276..>2149
                     /locus_tag="VFA14_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017454021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HZS80067.1"
                     /translation="MYSLTFSVLFAGFLLLTLVVRFWLGSRHIRHVLTHRSAVPAEFA
                     GKIPLAAHQKAADYTIARTRFGLLTRVANAAVLLGFTLLGGLQLLSDLLLHWFGNPGM
                     PYQLGLIAAFALISGLIDLPFDYYRQFVQEARFGFNRMTRGLFFADAIKNAAIGTVIG
                     LPLLWVILMLMDKSGSLWWFYAWLVWSGFQLLMLLLYPAVIAPLFNRFTPLDDDSLRM
                     RIEALMQRVGFASKGLFVMDGSRRSAHGNAYFSGFGAGRRIVFFDTLLTRLAPHEIEA
                     VLAHELGHFKLRHII"
BASE COUNT          458 a          570 c          586 g          535 t
ORIGIN      
        1 cacggagcgc aaccttaggt tgcaggactg aattccagga ggtggaaatt ggctgttggc
       61 gtgatcgata ccagtagaat cgaagcaatg gtggcgcaac tgaaagctgc tgctgcgcgg
      121 gggcaaggca atgttgcacc gatagaaaat atgagcgcta gcgaaaagca ggctacccgt
      181 gtcgattttg ccggtgtact gaaaaattca ctggatgaag tgaactccct ccaacaaaaa
      241 gctaaaaaaa tgtccgaaag cttctccatg ggaaacgacg atatcaatct gtcggacatg
      301 atgattgccg cacagaaagc gagtattgcc tttcagacca gtcttcaagt gcgcaataaa
      361 ctggtttccg cctatcgcga catcatgaca atgcaggtgt agccagcatt atcctgaaaa
      421 ccattgcggt gtgctgcgtt gtcccaatcc tcgacaggcc gccggcccgc ctgcgcttca
      481 cgccttgcat cccatcctga aaaaacgaca gtacgactcc cgatgagacc gtcaacgcgt
      541 tctgatgcac ccgcacacca tcgtgctatt ccttgatgaa gtgctcgcgg tagtatttca
      601 actcttcgat ggattcaatg atgtcagcca gtgcagtatg cttctgatgt ttcttgaaac
      661 cgcttgccag ctcaggcttc cagcgacggc acagttcctt gagcgtggaa acatcgaggt
      721 tacggtaatg aaaaaaggct tccagcctgg gcatgccgcg caccatgaag cggcggtcct
      781 ggcagatcga attgccgcac atcggcgatt tgccggccgg cacgaaatgc ttgaggaaat
      841 cgatcaatgc ttcttcagct tcggcctcgg tcacagtcga tgtcttgacg cgctcgatca
      901 ggccggaacg gccgtgggta cttttgttcc aggcatccat accgtcgagg atcgcatcgg
      961 actgatggat cgcaaatacc gggccttcgg ccaacacatt caatccggga tcagtcacca
     1021 caatagcaac ttcgatgatg cggtcgcggt ccgggtcgag gccagtcatt tccatgtcca
     1081 gccagatcag gttgagctcg ttcgggcgta ctgaagccgc gtaggccggc gcggcgcgat
     1141 gggtggcttg tgacataatg gctccttctt atcttatatg ttctgcttgg atcggctgat
     1201 tatcttattt gattcgaaac acgcatcgaa ttctttgaaa gattctgctg gtcgtcattt
     1261 tcgcacaggg aaagaatgta ttcactcacg ttttcggttt tgttcgccgg ttttttactg
     1321 ttgaccctgg tggtgcggtt ctggctcggt tcgcgtcata tccgccatgt gctgacgcat
     1381 cgcagcgcag tacccgccga atttgctggg aaaattccgc tggcagcgca tcaaaaagct
     1441 gctgactaca ctatcgcgcg taccagattc ggtttgttga cacgagtcgc caacgcagcc
     1501 gtactacttg gttttactct gctgggcggc ctgcaactac tgtcggatct actgctgcac
     1561 tggttcggca accctggcat gccttatcaa ttaggactga ttgcggcgtt tgctctgatt
     1621 tccggcctga ttgacctgcc tttcgactat taccgccagt tcgtgcagga ggcgcgtttc
     1681 ggcttcaaca ggatgacacg cggcctgttc ttcgccgacg ccatcaagaa cgcggcgatc
     1741 ggcacggtga tcggtctgcc actgttgtgg gtaatcctga tgttgatgga taaatccggg
     1801 tcattgtggt ggttttatgc gtggctggta tggagcggtt tccagctgct gatgctacta
     1861 ctgtatccgg ctgtgatcgc gccgctgttc aacagattca caccgctgga cgacgacagt
     1921 ctgcgtatgc gtatcgaagc gttgatgcaa cgtgtcggct ttgcctccaa gggcttgttc
     1981 gtgatggatg gttccaggcg cagcgcccac ggtaatgctt atttctccgg cttcggcgca
     2041 ggcaggcgca tcgtcttttt cgacacactg ctgacgcgtc tggcgccgca tgaaatcgaa
     2101 gcggtactgg cgcacgagct gggtcacttc aaactcaggc acatcatca
//
LOCUS       DASRDH010000054         7370 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_538374,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000054 DASRDH010000000
VERSION     DASRDH010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 7370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7370
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538374"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="VFA14_01340"
     CDS             complement(<1..251)
                     /locus_tag="VFA14_01340"
                     /inference="COORDINATES: protein motif:HMM:NF014126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HZS80068.1"
                     /translation="MKEVELSKPHTGGILPQVFYPSAGLVLALVSLSFFAPAQTQVLF
                     SAAKSWVVSEAGWFTVLSVAGFLVFVVALGFSSLGRLKLG"
     gene            579..2339
                     /locus_tag="VFA14_01345"
     CDS             579..2339
                     /locus_tag="VFA14_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006464311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HZS80069.1"
                     /translation="MNDELKNGHFPATNFLRGIIEADLVAGTYAARSDSHGQPLPQVV
                     TRFPPEPNGYLHIGHAKSICLNFGLARDYNGRCHLRFDDTNPEKEDQEYIDTIIDSVN
                     WLGFDWNHDGKQHLYYASNYFDIMYAGAQALIRQGHAYVDEQNAEQMRETRGTLTEPG
                     TDSPWRDRPTDESLRLFGEMRDGKHADGSLVLRARIDMASPNMNLRDPAIYRIRHAAH
                     HNTDNKWCVYPMYSYAHPIEDALENISHSICTLEFEDQRPFYDWVLERLSEEGFFKKP
                     LPHQYEFARLNLTYIVTSKRKLKQLVDEQIVNGWDDPRMPTIVGLRRRGYTPEAIRLM
                     CDRTGASKNNSWIDYSILEGALRDDLDPKTPRAVAVLRPLKLIIDNFPEGESVECSAP
                     VYPPAHPLHDSEYRCFPITRNVWIEQEDFMEVPTKGYFRLFPGNRVRLRYGYVVECTG
                     CDKDENGNVVAVHCNYFEDSKSGTSGSAHYKVKGNLHWVSAEHALKAEVRLYDRLFND
                     AHPDAGGKDFKLALNPHSKEVITACLEPTLKTAAAGALYQFERHGYFIADHVDSVAGK
                     PVFNRAVTLRDSWGKGDKRD"
     gene            2423..4159
                     /gene="dsbD"
                     /locus_tag="VFA14_01350"
     CDS             2423..4159
                     /gene="dsbD"
                     /locus_tag="VFA14_01350"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047134 - protein-disulfide reductase
                     (NAD(P)) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HZS80070.1"
                     /translation="MLLAGPLTGLILPTTAHAADYLAPEAAFKFSAGMVDAKTLEIHY
                     AIADGYYMYRDRFHFVAEGATLGPADFPHGSVKYDENFQKEVETYRHRVSIRIPIEAT
                     DRFTLKSTAQGCADQGLCYPPMESVAHILPEAIIKSIDADGAATDGKTDVAAGSDMHR
                     VTTVLKSGKLPAVLPLFFLLGIGLSLTPCVLPMVPILSSIIVGTSGQSSRGRGLLLSM
                     NYVLGMTLVYTALGITAGMIGEGLSAQLQHPWMLGLFALLMTGLSLSMFGFYQLQIPP
                     AWQSRLAQASRQSDGKLLGVFLMGMISALIVGPCVAAPLASVLLYIGQTHDVVLGGSA
                     LFAMALGMGSPLLVIGASAGVLLPRAGNWMESIQRFFGILMLATALWIVSPVIPAWLF
                     VAGWAALGIAYGAYLLWRMSAHWIARAFGLVFTALGLLQLLNVATGGRDVLAPLSQLH
                     GSNPGVATAPINFTRVRSIAELDVALAQSGGKPAMLDFYADWCVSCKEMEKFTFTDAR
                     VREKLSQMVLLQIDVTANNANDKAMLKHFQLFGPPGIILFDHRGRELSSKRVIGYQNA
                     DQFLQSLQAVLP"
     gene            4280..4918
                     /locus_tag="VFA14_01355"
     CDS             4280..4918
                     /locus_tag="VFA14_01355"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HZS80071.1"
                     /translation="MTLRLGDTAPNFKQDSSIGKIKFHEWAGDSWVVLFSHPADFTPV
                     CTTELGLTAKLKPEFDRRNVKAIALSVDDADAHNQWIKDIEDTQKTVVGFPIIADIDK
                     KVARLYDMIHPNQSETATVRALFIIDPNKKVRLILTYPMSTGRNFDEVLRVLDALQLT
                     DDCAVATPGNWKSGDDVIIPLSITDEETIKRKYPKGYTAPRPYLRITPQPDK"
     gene            complement(4951..5961)
                     /gene="hemB"
                     /locus_tag="VFA14_01360"
     CDS             complement(4951..5961)
                     /gene="hemB"
                     /locus_tag="VFA14_01360"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HZS80072.1"
                     /translation="MPSSIRSAAFPAMRMRRMRKDTFSRLLMQENAITSADLIYPVFI
                     QEGQNLRTPVGSLPGVERLSIDQLLRVAEDTVALGIPVLALFPVIAPALKTTDGIEAI
                     NPDGLVPRAVTALKQRFPELGVLTDVALDPYTSHGQDGVMDDNGYVLNDETTDFLVRQ
                     AQVQAQAGVDIVAPSDMMDGRIGAIRNAFEVQRFIHTRIMAYSAKYASAFYGPFREAV
                     GSAANLGKGSKNTYQMDPANSDEALREVALDLEEGADMVMVKPGMPYLDVVRCVKNEF
                     KVPTFAYQVSGEYAMIKAAAQNGWLDHDKAMMEAMMAFKRAGADGVLTYFARDIARCL
                     KK"
     gene            complement(6221..6925)
                     /gene="yihA"
                     /locus_tag="VFA14_01365"
     CDS             complement(6221..6925)
                     /gene="yihA"
                     /locus_tag="VFA14_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HZS80073.1"
                     /translation="MSQLWQARFFTTVNHLRDLPATQLPEIAFAGRSNAGKSSAINVL
                     CNQKKLAFSSKNPGRTQHINYFSIGGAQVGQHRKDETNVDEIRALLVDLPGYGYAEVS
                     GSAKLHWQELLGNYVRRREQLAALVMVIDARRPFTDLDIQMVEWFAPTGKPLHCILSK
                     ADKLNRHEAANALHQARTFLSSYVDADGKPFPFTAQLFSVLKRTGLDEANDRILGLLG
                     LLDDTPKDTEALSCRT"
     gene            complement(6936..7274)
                     /gene="fdx"
                     /locus_tag="VFA14_01370"
     CDS             complement(6936..7274)
                     /gene="fdx"
                     /locus_tag="VFA14_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HZS80074.1"
                     /translation="MPQIVIFPHAIFCPDGAVIDAPVGTTLCEAMLDNDIEIEHACER
                     SCACTTCHVVIREGFETLDAPSDKEEDLLDMAWGLEANSRLSCQVVLGDDDLVVEIPK
                     YTINHASENH"
BASE COUNT         1717 a         2141 c         1943 g         1569 t
ORIGIN      
        1 ccaagcttga gccttccgag gctgctaaac ccgagcgcca cgacgaacac gaggaacccg
       61 gccaccgaga gtacagtgaa ccagcctgct tcgctcacaa cccaggactt ggccgcgctg
      121 aacaacacct gtgtctgcgc aggagcaaag aacgacagcg acactagcgc cagcaccagg
      181 cctgcggagg gatagaacac ttgcggcaag atgccgccgg tatgcggctt cgacaattca
      241 acttccttca tgcgggttct cttattctct tatcacttct gcttttttgc cgcagccagc
      301 aatcaccagt gccaatgggc gcaaatatct tcttggctac cgtcaaggca tggcgagtca
      361 ttcatttcag tatattcttg atgcgtactg gaccgatcag gtcgatgcga tcagtgcaaa
      421 aaccatctac gctccaggca aaaaaccatt accgccattc tatgccagac gacaacggga
      481 tgttaccacc tgagccatcg ccgccggaaa agcccgtcaa tcctagcacg ctgcggtaaa
      541 atgcctgccc atgaaataag ttttcctgca aagcatccat gaacgacgaa ctgaaaaacg
      601 gtcactttcc cgccaccaat ttcctgcgcg gcatcatcga agccgacctc gttgccggca
      661 cctatgccgc acgcagcgac agccatggcc agcccctacc gcaggtagtg acccgctttc
      721 ctcccgaacc caacggctat ctgcacatcg gccatgccaa gtcgatctgc ctcaatttcg
      781 gcctggcgcg cgattacaac ggtcgctgcc acctgcgctt cgacgacacc aatccggaaa
      841 aagaagacca agaatatatc gataccatca tcgactcggt caactggctg ggcttcgact
      901 ggaaccacga tggcaagcaa cacctgtact acgccagcaa ttacttcgac atcatgtatg
      961 caggcgcaca ggcgctgatc cgtcaaggcc atgcctacgt cgacgagcaa aacgccgaac
     1021 aaatgcgtga aacgcgcggt acgctgaccg aacccggtac cgactctcct tggcgcgacc
     1081 gaccaaccga cgaatcactg cgcctgtttg gagaaatgcg cgacggcaaa catgccgacg
     1141 gcagtctggt tctgcgcgcc aggatcgaca tggccagtcc caatatgaat ctgcgtgacc
     1201 ccgccatcta ccgcatccgc cacgccgcgc atcacaacac tgacaacaaa tggtgcgtct
     1261 atccgatgta tagctacgcg catccgatcg aagacgcgct ggaaaacatc agccactcaa
     1321 tctgcacact ggaattcgaa gaccagcgcc cattctacga ctgggtgctg gaacgcctga
     1381 gcgaagaagg tttcttcaaa aagccgctac cacatcaata cgaatttgcc cgtctcaacc
     1441 tgacttacat cgtcaccagc aagcgcaagc tcaagcaact ggtcgacgaa cagatcgtca
     1501 acggctggga cgatccgcgc atgcctacca tcgtcggcct acgccgccgc ggttatacgc
     1561 ctgaagcgat ccgcctgatg tgcgaccgca ccggcgcttc caagaataat agctggatcg
     1621 actacagcat cctcgaaggc gcgctgcgcg acgacctcga tcccaaaaca ccgcgcgcag
     1681 tcgccgtatt gcgtccgctc aagctgatca tcgacaactt cccggaaggc gaaagcgtcg
     1741 aatgcagcgc accggtctat ccgccagcgc atccgctgca tgacagcgag tatcgctgct
     1801 tcccgatcac caggaatgta tggatcgagc aggaggactt catggaagtg ccgaccaaag
     1861 gctacttccg cctttttccc ggcaacaggg tacgcctgcg ctacggctac gtcgtcgaat
     1921 gcactggctg cgacaaggat gaaaacggca acgtcgtcgc cgttcactgc aactacttcg
     1981 aagacagcaa aagcggcacg tccggcagcg ctcactacaa agtcaaaggc aacctgcact
     2041 gggtcagcgc cgaacacgcg ctaaaagctg aagtgcgtct atacgaccgc cttttcaatg
     2101 acgcacatcc ggacgccggc ggcaaagatt tcaaattggc cttgaatccc cattcgaagg
     2161 aagtcatcac tgcctgtctc gaaccgacgc tcaagaccgc cgcagccggc gccctctatc
     2221 agttcgagcg tcacggctac ttcattgccg atcatgttga ctcggtagcc ggcaaaccgg
     2281 tattcaatcg cgccgtgaca ctcagggaca gctggggcaa aggggataag cgagattgac
     2341 catgttgtcg ctggccgcat acgtaccggt tttttcgact atcgccatac gtggtgagct
     2401 agtgaaaaaa tggatggcgg cgatgctgct ggctggaccg ttgacagggt tgatactccc
     2461 cacgacagcg catgcagcgg attacctcgc gcccgaagcg gcgttcaaat tctcagccgg
     2521 gatggttgat gccaagacgc tggaaatcca ttacgcgatt gctgacggtt attacatgta
     2581 ccgcgaccgc tttcatttcg ttgcggaggg cgcgacgctg ggaccagccg attttccgca
     2641 tggcagcgtc aagtatgatg aaaattttca gaaggaagtc gaaacttacc gccatcgtgt
     2701 cagcatccgc atcccgatcg aagccaccga tcgattcaca ttgaaatcaa cagcgcaggg
     2761 atgcgccgac cagggcttgt gctatccacc gatggaaagc gtggcgcaca tattgccgga
     2821 agcgatcatc aagtccatcg atgccgatgg cgctgctact gacggcaaga ccgatgtagc
     2881 agccggctcc gacatgcaca gagttaccac tgttctcaag agcggcaagc tgcccgctgt
     2941 gctgccacta ttcttcctgc tgggcatcgg tttatcgctc acgccgtgtg tgttgcccat
     3001 ggtgcctatc ctgtcatcca tcatcgtcgg cacgagcgga caatcgagcc gcgggcgcgg
     3061 cttgttattg tcgatgaatt atgtactcgg catgacgctg gtttataccg cgctgggtat
     3121 caccgccggc atgatcggcg aaggtctgtc ggctcaattg cagcatccgt ggatgctggg
     3181 cctgttcgcc ttgctcatga cagggctgtc actgtcgatg ttcggtttct atcaattaca
     3241 gataccgccg gcatggcaat ccagactcgc gcaggcatcg cggcaaagcg atggcaagct
     3301 gctgggcgta ttcctcatgg gtatgatttc ggcattgatc gtcggtcctt gcgtcgcagc
     3361 accactagcc agtgtattac tctatatcgg ccagacgcat gacgttgtgc tcggcggcag
     3421 cgccctgttc gcaatggcac tcggcatggg gtcgccattg ctggtcattg gtgcgtcggc
     3481 tggcgtcctg ctgccgcgtg ccggcaactg gatggaatcc atccagcgct tcttcggcat
     3541 actcatgctg gcaacggcat tatggatagt gtcgccggtg attccagcgt ggctgttcgt
     3601 tgccggctgg gctgctttgg gtatcgccta tggtgcttac ctgctgtggc gcatgtcggc
     3661 gcactggatt gcgcgcgcct tcggtctggt atttacagca ttgggattac tgcaactgct
     3721 caatgtcgcc actggtggcc gcgatgtcct ggcgccgctg tcacagttgc atggcagcaa
     3781 tccaggcgta gctaccgcgc ctatcaattt cacacgtgtc agatcgattg ccgagctgga
     3841 cgttgcattg gctcagagcg gcggcaagcc ggccatgctc gatttttatg ccgactggtg
     3901 cgtctcttgc aaagagatgg aaaaattcac cttcaccgat gcgcgtgtgc gtgaaaagct
     3961 gtcgcagatg gtattgctgc aaatcgacgt caccgccaac aatgccaacg acaaagccat
     4021 gctcaaacat ttccagctgt tcgggccacc gggcattatt ctgttcgacc atcgaggccg
     4081 ggaactctcc agcaaacgtg tgatcggtta tcaaaatgcc gatcaattcc tacaaagttt
     4141 acaagccgtg ctgccataat atgcaacgcg gcgtcatagc ctgacactat ctggctgcgc
     4201 attaatagtc attggcactg aaaatggcga gcgctatagt aaacggcctg tattctattt
     4261 tcaccacaag gaaattgaaa tgacactacg tttaggcgat actgcgccaa atttcaagca
     4321 agactccagc atcggcaaaa tcaaattcca tgaatgggcg ggcgattcct gggtcgttct
     4381 attctcgcac ccggccgatt tcacaccggt ctgtaccacc gaactgggcc tgacggccaa
     4441 actcaagcca gaattcgaca gacgtaacgt caaggccatt gccttgtcgg tcgatgatgc
     4501 cgatgcacac aatcaatgga tcaaggatat cgaagacaca caaaagaccg tggtcggctt
     4561 ccccatcatc gccgacatcg acaaaaaagt cgcccgtcta tacgacatga tccatccgaa
     4621 ccaatcggaa accgcgactg tacgcgcgct attcatcatc gatccgaaca agaaagtgcg
     4681 actgatcctc acctacccga tgagtaccgg ccgcaacttc gacgaagtgc tgcgcgtact
     4741 ggacgcactg caactgactg acgactgcgc cgtcgccacg ccaggcaact ggaaaagcgg
     4801 cgatgacgtc atcatcccgc tgtcgatcac ggatgaagaa accatcaaac gcaaatatcc
     4861 gaagggctac accgcgccgc gtccttacct gcgcatcacg ccgcagccgg acaagtgacg
     4921 cgtattgcct gaaaaaagca ctccagcttt ttacttcttc agacaacgcg cgatatcgcg
     4981 ggcgaaataa gtcagtaccc catcggcgcc ggcgcgcttg aaggccatca ttgcttccat
     5041 catcgccttg tcgtgatcga gccagccatt ttgcgcagcg gccttgatca tggcgtattc
     5101 gccgctaacc tgataagcga aggtcggcac cttgaattcg ttcttcacac agcgcacgac
     5161 gtcaagataa ggcataccag gtttgaccat taccatgtcg gcaccttctt ccagatccag
     5221 cgcgacttcg cgcagcgctt catcgctatt ggccgggtcc atctgataag tgttcttgct
     5281 gcctttaccg aggttggcgg ctgaaccgac cgcctcgcgg aacggaccgt agaaagccga
     5341 tgcatatttg gccgaatacg ccatgatgcg tgtatgaatg aaccgctgta cttcgaacgc
     5401 attgcggatg gcaccgatgc gaccgtccat catatccgag ggcgctacaa tgtcgacgcc
     5461 ggcctgcgcc tgcacctgcg cctgcctgac caggaaatcg gtagtttcgt cgttgaggac
     5521 atagccgttg tcatccatca caccatcctg gccatggctg gtatacgggt cgagtgcaac
     5581 gtcggtgaga acgcccagct cggggaagcg ctgtttcagc gcggtgacag cgcgcggcac
     5641 cagaccatca gggttgatgg cttcgatgcc gtctgtcgtt ttcaacgctg gggcgataac
     5701 tggaaacaag gccagcaccg gaataccaag cgcgaccgtg tcttcggcaa cacgcagcaa
     5761 ctgatcgatg gacaagcgct cgacgccggg cagcgaaccg accggcgtgc gcagattctg
     5821 gccttcctgg ataaagaccg gatagatcag atcggcagag gtgatggcgt tttcctgcat
     5881 cagcagacgg gaaaacgtat ctttgcgcat acggcgcata cgcatggcgg gaaaagcagc
     5941 agacctgatg gaagatggca tatgagggtg ctccttaaag gtacacagca cgttacaaat
     6001 aacgttaaat tactgataag tatgaataac ttatattaaa aaatagttaa atgacgataa
     6061 ttgagaaatc catatacaga ttgaaacttt tcctcagagc aggctctcct acgcataggc
     6121 aatgtatatt gcctcccgct tccccttccc tccctgagcg gggttatcgt gcaaacgatg
     6181 gcatttgccc tggaaaggcc tcctcctttt cagggcattt ttatgtccgg catgacagtg
     6241 cttcagtatc tttcggcgta tcgtccagta atcccagtag tcccagaatc ctgtcattgg
     6301 cttcgtccag gccggtacgt ttgagtacgg aaaacaattg cgccgtgaac gggaacggct
     6361 tgccatcggc atcgacgtag ctggatagga aagtgcgcgc ctgatgcagg gcattggcgg
     6421 cttcgtgacg gttgagcttg tcagccttgc tgagaatgca atgcagtggc ttgccggtcg
     6481 gcgcaaacca ctccaccatc tgtatatcga gatcggtaaa cggccggcgc gcatcgatga
     6541 ccatgaccag cgcggcgagt tgctcacggc gacgcacata gttaccaagc agttcttgcc
     6601 agtgcaattt ggccgaaccc gacacttccg catagccgta gccaggcaag tcaaccagca
     6661 aggcgcggat ttcgtcaaca ttggtctcgt ccttgcgatg ctgtcctacc tgtgcgccac
     6721 cgattgaaaa ataattgata tgctgggtcc gtccaggatt tttcgatgaa aatgccaatt
     6781 ttttttgatt acataataca ttgatggcgc tagatttacc ggcattggaa cgaccggcga
     6841 aggcgatttc aggcaactgt gtggccggca aatcacgcag atgattgacc gtggtgaaga
     6901 aacgggcttg ccaaagttga gacatgacag gaggttcagt ggttctcact ggcgtggttg
     6961 atggtgtatt tcgggatctc gaccaccagg tcatcgtcgc ccagcacgac ttggcacgac
     7021 agacgcgaat tcgcttccag gccccaggcc atgtccagca aatcttcttc cttgtcggag
     7081 ggggcatcga gcgtctcgaa accttcacgg atcaccacat ggcaggtggt gcaggcacat
     7141 gatctttcac aggcgtgttc gatctcgatg tcgttgtcga gcatggcttc gcacagcgtc
     7201 gtgccgaccg gtgcgtcgat gacggcgcca tcagggcaaa aaatggcgtg tgggaatatg
     7261 acgatttgtg gcacggtatt gacctttgtc cttacaaaat ttcatccagt ttcctgccgg
     7321 tcagcgcggc atggatgctg cggtccatac ggcgggcagc aaactcttcg
//
LOCUS       DASRDH010000055         5034 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1478068,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000055 DASRDH010000000
VERSION     DASRDH010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 5034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5034
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1478068"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..664
                     /locus_tag="VFA14_01375"
     CDS             <1..664
                     /locus_tag="VFA14_01375"
                     /inference="COORDINATES: protein motif:HMM:NF019625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class II D-tagatose-bisphosphate aldolase,
                     non-catalytic subunit"
                     /protein_id="HZS80075.1"
                     /translation="AWQRVIGLVVQPGVEFDHLSVVDYQASLASGLSGLLDTHAGIVF
                     EAHSTDYQTPSALCDLVRDGFAILKVGPGLSFALREAVYGLAAIECLLVPAASRSNML
                     ETLERSMLARPQHWQSHCHGDAAMQRLLRHYSYSDRIRYYWPEPEVETAFRILLSNLE
                     RVSIPDPLLSQYFPEQYQKLRHRTLDGSARSLLIDRIQQAIEPYARACSAQTPDGNKH
                     HH"
     gene            678..1460
                     /locus_tag="VFA14_01380"
     CDS             678..1460
                     /locus_tag="VFA14_01380"
                     /EC_number="1.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013090397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-iditol 2-dehydrogenase"
                     /protein_id="HZS80076.1"
                     /translation="MARLQEKHAILTGAGGGIGLAVAAAYLKEGARCTVADISATPSA
                     ALAALMKRYPETLMYVAADVTLPSSVERLIECASQSFGPVDVLFNNAALFDMAPLLES
                     EEAMYDRLFAVNVKGLFFTMQKVLAQMVRLGTRGAVINMASQAGRRGEALVSHYCATK
                     AAVISYTQSAALAMAASGIRINAIAPGVVDTPMWDHVDALFARYEGLQPGEKKIAVGK
                     AVPLGRMGRPDDIAGAAVFLASAESAYITAQTLNVDGGNWMS"
     gene            1589..3709
                     /locus_tag="VFA14_01385"
     CDS             1589..3709
                     /locus_tag="VFA14_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HZS80077.1"
                     /translation="MHTDTSATAVRRELRGRILLVTIDNPPVNALGAEVRRDLLSTIE
                     AADADHAIAAVCITGAGRDFIGGADIREFGKPPLPPSLPDLCNRIEACSKPVVVAIHG
                     AALGGGLELALAAHYRLAIAGARIGLPEVHLGLLPGAGGTQRAPRLIGARAALDMMLN
                     GRHLSAEEACALGLVDRLSTDDALADGLAYTQELLAMHAPARRTRDAGALTDDQTANR
                     AALADARAKTSKKSRALFSPLKIIEAVEAAITLPFDDGLRLERKLFLQCMDSPQRAGL
                     IHAFFAERAVRKAPEIHDAKPRAITSVGVVGGGTMGAGIAVTVLDAGLPVTMIERDEA
                     ALARGRAYVEQVYDGLIAKGRMSAEAKHATMARLTGSITCDALAQVDLVIEAVFEDMS
                     VKQAVFAELDHVCKTGAVLATNTSYLDIDAIAAGISRPADVIGLHFFSPANIMKLLEI
                     VVPARVSADVVATAFELAKKLRKVPVRAGVCDGFIGNRILSVVRQAADYMMEDGASPY
                     QIDQAVRAFGYPMGPFQVADLAGGDIGWATRKRKAATRDPRTRTVQIADRLCERGWFG
                     QKTGRGYYLYPDGARAGTPDPEVLAIIDTERARAGIMPRSFTDDEIIQRYMAAMINEG
                     ANVVHQCIALRPLDVDVCFLYGYGFPRYRGGPMQYADLTGLPQVLADIRAFAREDALF
                     WQPSPLLLDLVGRGTDFASLNQAE"
     gene            3726..4922
                     /locus_tag="VFA14_01390"
     CDS             3726..4922
                     /locus_tag="VFA14_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZS80078.1"
                     /translation="MDLHLSAKEEAFRNEVRAFLAEQLPARLSGKVASGKHLSKADMQ
                     EWHAILDARGWLASHWPEKYGGTGWNAVEKFLFEHECCLADAPRIVPFGVNMLGPVLI
                     RYGSQAQQQHWLPRILSGADWWCQGYSEPGAGSDLAALQTAAVRNGDHYVVNGQKTWT
                     TFGQYANMIFCLVRTSRAAKKQDGISFLLIDMATPGVEVRPIITLDGEHEVNEVFFTD
                     VRVPVGNLVGAENQGWTCAKYLLTYERANIAGVDFAVAALARLKRIAAQQVRNGQPLA
                     DDPSFAARLARVEIDLDNMKTTNLRMIAAAAGGDAPGAESSMLKLRGTEIRQEITSLT
                     RRAMGPYARPYLIEALEDDYHGPQIGPVAAASAAAQYFNYRKLSIFGGSNEIQKNIIS
                     RMILRL"
BASE COUNT         1000 a         1598 c         1523 g          913 t
ORIGIN      
        1 tgcctggcaa cgggtcatcg gtctagtcgt acagccgggc gtcgagttcg atcatctgtc
       61 ggtggtcgat tatcaggcgt cgctggcatc cggcttgagc ggcctgctcg acacccatgc
      121 cggcatcgtg tttgaagcgc actcgaccga ttatcagacg ccctccgcct tgtgcgatct
      181 ggtgcgtgac ggcttcgcca tcctcaaggt cggaccagga ctgagcttcg cactgcgtga
      241 agcggtgtat ggactcgctg ccatcgaatg cttgctggtt cccgcggcga gccgttcgaa
      301 tatgctggaa acactggagc gcagcatgct cgcccggccg cagcactggc agtctcactg
      361 ccatggcgat gcggccatgc agcgactgct gcgccattac agttacagcg accggatccg
      421 ttactactgg cctgaacctg aagtggagac agctttccgc atcctgttga gcaatctgga
      481 gcgggtttcc attcctgacc ctctgttgag ccaatatttt ccagagcaat atcagaaact
      541 gcgtcaccgg accctggacg gctctgcgcg atcactgctg atcgacagga tccagcaagc
      601 catcgaacct tatgcgcggg catgctccgc tcaaacgccc gatggaaata aacaccatca
      661 ttgaaaggaa acgcaccatg gctcgtcttc aggaaaagca tgccattctc acgggggcag
      721 gtggcggtat cggcctggct gtcgcggcag cctatttaaa ggaaggggcg cgctgcacag
      781 tggcggatat ttccgccacg ccatcggcag cgctggcggc gctgatgaag cgctatccgg
      841 agacgctgat gtatgtcgct gccgatgtca ccctgccctc ttccgtcgaa cgactgatcg
      901 aatgtgccag ccagagcttc gggccggtgg atgtattgtt caacaatgcg gccctgtttg
      961 atatggcgcc gctgctggag tcagaagaag cgatgtatga ccggctgttc gcagtcaacg
     1021 tcaaaggcct gtttttcacc atgcaaaaag tgctggcgca gatggttcgc ctgggcaccc
     1081 gtggcgcagt aatcaatatg gcatcgcagg cgggacggcg cggagaagcg ctggtgtcgc
     1141 attattgcgc gaccaaggcc gccgtcatca gctataccca atccgccgcg ctggcgatgg
     1201 ctgcctcagg catacgcatc aatgcgattg cccccggcgt ggtcgatacc ccgatgtggg
     1261 accatgtcga tgcgctgttc gcacgctatg aaggcttgca gcccggagaa aagaaaatcg
     1321 ccgtcggcaa ggccgtgcca ctgggccgca tggggcgccc tgacgacatc gccggcgccg
     1381 ccgttttcct tgccagtgcg gagtctgcct acatcacggc ccagaccctg aatgtggatg
     1441 gcggcaactg gatgagctga tggcctcgcg ttttaccgcg cgagccagac tgacatgcag
     1501 cagacggcat gacatcgacc gctatatcgc gcctggtacg cgaaccaaag ttgcgccata
     1561 cggaccaata ccacaggaga cgatcataat gcacactgac acatctgcta ctgccgtgcg
     1621 gcgcgaattg cgtggccgga ttctgctggt aaccatcgac aacccgccgg tcaatgcttt
     1681 gggcgcggag gtgcggcgcg accttctcag cacgatagag gccgccgatg ccgatcacgc
     1741 catcgcggca gtgtgcatca ccggcgcggg gcgcgacttt atcggcggcg ctgatatccg
     1801 cgagttcggc aagccgccgc tgccgccatc actgccggat ctctgcaacc ggatcgaggc
     1861 ttgcagcaag ccggtagtcg tggcaatcca cggcgcggcg ctgggcggcg gactggagct
     1921 ggcgctcgca gcgcactacc ggctggcgat cgccggcgcc aggatagggc tgccggaagt
     1981 ccatctcggc ctgctccccg gcgccggcgg aacacagcgc gcgccgcgtc tgatcggcgc
     2041 cagggcagcc ttggacatga tgctcaacgg acgccatctg agcgcggaag aagcctgtgc
     2101 gctcggcctg gtcgaccgtc tcagtactga cgatgcactg gccgatggcc tggcgtatac
     2161 gcaggaattg ctggccatgc acgcgccggc gcgccgcacg cgcgacgccg gcgcattaac
     2221 cgacgatcaa acagcgaacc gcgccgcgct tgccgacgcg cgcgccaaaa ccagcaaaaa
     2281 atcgcgcgct ctgttctcgc cgctaaagat catcgaggcc gtcgaagcag ccatcactct
     2341 cccgttcgat gatggtctgc gcctcgaacg caaactgttt ctgcaatgca tggacagtcc
     2401 gcaacgcgcg gggctgattc atgccttctt cgccgagcgt gcagtgcgca aagcccctga
     2461 aatccatgac gcaaaaccac gcgccatcac cagtgtgggt gtggtcggtg gcggcactat
     2521 gggcgccggc atcgcggtga cggtgctgga cgccggcctg cccgtgacga tgatagaacg
     2581 tgacgaagcg gcgctggcgc gtggccgcgc gtacgtcgag caggtatatg acggtttgat
     2641 cgccaagggc cgcatgtccg ctgaagccaa acacgccacg atggcgcgcc tgaccggcag
     2701 catcacctgc gatgctcttg cccaggtcga tctggtgatc gaagccgtgt tcgaggacat
     2761 gtcggtcaag caggccgtgt tcgccgaact cgatcacgtc tgcaagaccg gcgccgtact
     2821 ggccaccaat acctcctatc tcgatatcga cgccatcgcc gctggcatct cccgtcccgc
     2881 ggacgtcatc ggactgcact tcttttcacc ggccaacatc atgaaactgc tcgaaatcgt
     2941 ggttccagcc cgggtttcgg cagatgtcgt cgctaccgca ttcgaactgg cgaaaaagct
     3001 gcgcaaggtg ccagtacggg ccggcgtgtg cgacggtttc atcggcaacc gcatcctctc
     3061 ggtggttcgc caggccgccg actacatgat ggaagacggc gcttcgccct accagatcga
     3121 ccaggcggtg cgcgcattcg gttatcccat gggtccgttc caggtagccg acctggccgg
     3181 cggcgacatc ggctgggcca cccgcaagcg caaggcagct acccgcgatc cgcgcacgcg
     3241 caccgtgcag attgccgacc gcctctgcga gcgcggctgg ttcggccaga aaaccggacg
     3301 cggctactat ctctaccccg acggcgcgcg cgccggaacg cccgacccgg aagtcctggc
     3361 aatcatcgat accgaacgcg cgcgggccgg catcatgccg cgcagtttca ccgacgatga
     3421 aatcatccag cgttatatgg cggcgatgat caacgaaggc gccaatgtcg tccaccagtg
     3481 catcgcactg cggccactcg atgtcgacgt ctgctttctg tacggttacg gctttccgcg
     3541 ctatcgcggc ggcccgatgc aatatgccga cctgaccggt ctgccgcagg tgctggccga
     3601 catccgcgcc ttcgccaggg aagatgcgct gttctggcag ccttcgccat tgctgctcga
     3661 tctggtcggg cgcggtacgg atttcgcctc actcaatcaa gctgaatgat gcgaggagta
     3721 tcgacatgga cttgcatctt agcgccaaag aagaagcctt ccgcaacgaa gtgcgcgcct
     3781 ttctggccga acaactgccc gcccgcctgt ccggcaaggt cgccagcggc aaacatctca
     3841 gcaaagccga tatgcaggag tggcacgcga ttctcgacgc acgcggctgg ctcgccagcc
     3901 actggccgga gaaatacggc ggcacaggct ggaacgctgt cgaaaaattc ctctttgaac
     3961 atgaatgctg cctcgccgac gccccgcgca tcgtgccttt cggcgtcaac atgctggggc
     4021 cagtgctgat cagatacggc agccaggcgc aacagcagca ttggctgccg cgcatcctca
     4081 gcggcgccga ctggtggtgc cagggctact cggaaccagg cgcgggatcg gacctggccg
     4141 cgcttcagac tgctgccgtc cgcaacggcg accactacgt cgtcaacggc cagaaaacct
     4201 ggaccacgtt cggccagtac gccaacatga tcttttgcct ggtgcgcacc agccgcgcag
     4261 ccaaaaaaca ggatggcatc agtttcctgc tgatcgatat ggcgacgcca ggcgtcgaag
     4321 tacgcccgat catcacgctc gacggcgaac acgaagtgaa tgaagtgttt ttcaccgatg
     4381 tccgcgtacc ggtcggcaat ctggtcggcg cagaaaacca gggctggacc tgcgccaaat
     4441 acctgctgac ttacgagcgc gccaatattg ccggggtcga tttcgcggtc gcggcgctgg
     4501 cgcggctcaa acgcatcgca gcgcaacagg tacgcaacgg ccagccgctg gcggatgatc
     4561 catcgttcgc ggcccgtctg gcgcgcgtcg aaatcgacct cgacaatatg aagacgacca
     4621 atctgcgcat gatcgcggcg gcggccggcg gcgatgcgcc cggtgcagaa agttcgatgc
     4681 tgaagctgcg cggcaccgaa atccgtcagg aaatcacctc gctgacacgc cgcgccatgg
     4741 gtccctatgc gcggccttac ctcatcgaag cgctggagga cgactaccac ggaccgcaga
     4801 tcggccctgt ggcggcagcc agcgcagcag cgcagtattt caactatcgc aagctatcga
     4861 tcttcggcgg ttccaacgaa atccagaaaa atatcatctc caggatgatt ctcaggctgt
     4921 gagaacctgt tcatgatctg gaacgtgaac aggctctaag gaaacggaca tgaactttga
     4981 gcacacagaa gaacggcgca tgctggccga tagcctgaac cgcttcatcg ccgg
//
LOCUS       DASRDH010000056         4118 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_84491,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000056 DASRDH010000000
VERSION     DASRDH010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 4118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4118
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84491"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..654
                     /locus_tag="VFA14_01395"
     CDS             <1..654
                     /locus_tag="VFA14_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007882283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HZS80079.1"
                     /translation="SIVYFLVTDTEEMLRQKIIDHPHSRFLVCENNIDDVLGYIDSKD
                     VLKRLLKGEQFSLRDKGLIHISLSIPDSLNLWEVLERMKAVDEDLAVVVNEYALVVGI
                     ISITDVTSVLMGNLLNMSSEEQQIVKRDEHSWLMDGLTPLAEAMNALCIDDYPNPMGY
                     ETLSGFIMYMLRKIPKKTDFALFADYKFEVVDIEGNRINQLLVTRFKPEKSDKFEQE"
     gene            complement(668..1471)
                     /gene="fliR"
                     /locus_tag="VFA14_01400"
     CDS             complement(668..1471)
                     /gene="fliR"
                     /locus_tag="VFA14_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007882595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HZS80080.1"
                     /translation="MINFTSAQLSVWIASFIWPLTRILGLLSIAPPFGSASVPMLVKI
                     LLGIMISLVIAADVPVPPALDPISMTGLMILAQQFIIGTAMGFAVRLAFAATEMAGEL
                     TSHTMGLGFAVFFDPQSQGRTSAISQLFSLLATLVFLSINGHLILIAVMADSFHTLPI
                     TAAQVTAEGFHYLVLSGTRIFSIGLQLALPILVTLLITNIALGILTRAAPQLNLFGIG
                     FPVTIVVGFLLISICLPYMLTPIRYLLDNAIELVRVLGGMATIPPMPRH"
     gene            complement(1526..1795)
                     /gene="fliQ"
                     /locus_tag="VFA14_01405"
     CDS             complement(1526..1795)
                     /gene="fliQ"
                     /locus_tag="VFA14_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006462668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HZS80081.1"
                     /translation="MTPEHAMSLGRHAVEVTLMIAAPMLLTALIIGLCVSIFQAATQI
                     NEVTLTFIPKLVGVFLALTIAGPWMISIMMDYMRLMFSSIPAMVQ"
     gene            complement(1833..2600)
                     /gene="fliP"
                     /locus_tag="VFA14_01410"
     CDS             complement(1833..2600)
                     /gene="fliP"
                     /locus_tag="VFA14_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007882590.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HZS80082.1"
                     /translation="MTAASTSSALPHRWLHLLPAFLLCLPLAAVAQGLPAFTSTPTAG
                     GGQTYNLSIQTMLLLTSLSFIPAALLMMTSFTRIIIVLALLRHALGTNSTPPTQVLLG
                     LALFLTLFVMGPVFDRIYVDAYQPFSENKISMADAMEKGAAPLKEFMLKQTRQSDLAL
                     YAKLSRSPELQGPEDVPLRLLIPAFITSELKTAFQISFAIFIPFLIIDMVVASILMSM
                     GMMMMSPVIVSLPFKLMLFVLVDGWQLLVGSLAESFY"
     gene            complement(2587..3066)
                     /gene="fliO"
                     /locus_tag="VFA14_01415"
     CDS             complement(2587..3066)
                     /gene="fliO"
                     /locus_tag="VFA14_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007882589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HZS80083.1"
                     /translation="MKRFHRLAQARAYYSRHRSFIAVSASFIAGVAQAADQQAPATAT
                     LPSATGSLVTMLLGLIAVLALMAGIAWIFKRSGLTPGAGSNAITKIIGGINVGTRERV
                     MVIEVADQWIVVGVAPGRVNTLTTMPRQEHVAVSTSTMSTNFTSWLKHTIDKRNDGS"
     gene            complement(3063..3518)
                     /gene="fliN"
                     /locus_tag="VFA14_01420"
     CDS             complement(3063..3518)
                     /gene="fliN"
                     /locus_tag="VFA14_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006462664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HZS80084.1"
                     /translation="MAEENVDQTAAKVDPQGAAPAEPAPAEKEGSAAKPARKSSKATQ
                     AAEAGVFKELGGTGQRANDALNDIDLILDIPVQLTVELGRTKIAIKNLLQLAQGSVVE
                     LGGMAGEPMDVLVNGCLIAQGEVVVVNDKFGIRLTDIITPAERIRKLNR"
     gene            complement(3511..>4118)
                     /locus_tag="VFA14_01425"
     CDS             complement(3511..>4118)
                     /locus_tag="VFA14_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007882587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HZS80085.1"
                     /translation="FPIRVEGRAFTPTEQRIIMCILDIVTENYAKSWEPIYPIKFEYI
                     RSELNTQFANIATPNEIVVSTTFTMNLGSVSGEIHICLPYSMIEPIRDKLTSSIQGEA
                     LEIDKRWIRLMKQQIQAAEVELIANFGTAQLTFTDLLRMQVGDVIPLQPGESVVAQVD
                     GIPVMECSYGQLNGQYALRVEKLLSIAQQETLESIQGEQNG"
BASE COUNT          980 a         1050 c         1196 g          892 t
ORIGIN      
        1 agcatcgtct attttctggt gaccgatacc gaagaaatgt tgcgccagaa aatcattgat
       61 catccgcact cgcgcttcct ggtctgtgaa aacaatatcg acgacgtact cggctacatc
      121 gatagcaagg atgtactcaa gcgtttgctc aaaggcgagc agttttccct gcgcgacaaa
      181 ggcctgatcc atatatcact atcgatcccc gacagcctga atctatggga agtgctggaa
      241 cgcatgaaag ccgtcgacga agacctcgcc gtggtcgtca acgaatatgc gctcgtggtc
      301 ggcatcatca gcatcaccga cgtaaccagc gtgctgatgg gtaatctgct gaacatgtcg
      361 tcggaagagc agcagatcgt caagcgcgat gaacactcct ggctcatgga tggcctcacg
      421 ccgctggcgg aagcgatgaa cgcgctctgc atcgatgact atccgaatcc gatgggatac
      481 gaaacgctgt ccggtttcat catgtatatg ctgcgcaaga ttccgaagaa aacggatttc
      541 gctttgttcg ccgattacaa attcgaggtt gtcgatattg aaggcaacag aatcaatcag
      601 ctcctggtga caagattcaa accggagaag agcgacaaat ttgagcagga atgatcacga
      661 tgacagatca atgcctgggc atgggaggaa tggtcgccat gccgcctagc acgcgtacca
      721 gttcgatggc gttgtcgagg agatagcgta ttggtgtgag catgtaaggt agacagatgg
      781 agatgagcag gaagccaacc acgatggtga caggaaaacc aatgccgaac agattcagtt
      841 gcggcgctgc acgtgtcagg atgcccaggg cgatattggt gatcagcaaa gtcaccagta
      901 tcggcagcgc taattgtaag cctatgctga aaatgcgcgt gccgctcagc acgagatagt
      961 gaaaaccttc cgccgtaacc tgtgctgcag taatggggag tgtatggaag ctatcggcca
     1021 tcaccgcaat tagaatcagg tggccgttga tgctcaggaa caccagcgtg gccaggaggg
     1081 agaacaactg gctgatggcg gatgtgcggc cttgtgattg cggatcgaaa aacactgcga
     1141 agccgagacc catggtgtgg ctggtgagtt ctccagccat ttcggtggcg gcgaatgcta
     1201 gacgaacggc aaaacccatg gcggtaccga taatgaattg ctgggccaga atcatcaggc
     1261 cggtcattga tatcgggtcc agtgccggcg gtacaggaac atctgccgcg atcaccagag
     1321 agatcatgat acccagcagg attttgacca gcatggggac gctggcgctg ccgaagggcg
     1381 gcgcgatcga cagcagtccc agaatgcggg tcaagggcca gatgaaggag gcaatccaga
     1441 cagagagctg ggcgctggta aagttgatca tgatgtcctc gattgcagaa aatttccgca
     1501 atcatcggtt agagcgtgtt atggactatt gcaccatcgc gggaatgctg ctgaacatca
     1561 ggcgcatgta atccatcatg atgctgatca tccaggggcc ggcgatggtc agcgccagga
     1621 acacgccgac cagcttggga atgaaagtca acgtgacttc attgatttgt gttgcggcct
     1681 gaaaaatact gacacacaga ccgatgatca atgccgtcag caacatcggc gcggcaatca
     1741 tcaacgtgac ttcgacggca tggcggccaa gagacattgc atgttcagga gtcatgaatt
     1801 taccttagag cgtgttatgg accatttcat agctagtaaa aactttccgc cagcgaacca
     1861 accagcaact gccagccgtc gaccagtacg aacagcatca gtttgaaggg cagcgacacg
     1921 atgactggcg acatcatcat catccccatc gacatcagga tactggcgac taccatgtca
     1981 atgatcagaa agggaatgaa aatggcaaaa ctgatctgga acgccgtctt gagttcgctc
     2041 gtgatgaacg ccggaatcag caggcgcagt ggcacatcct ccggaccttg cagctcaggg
     2101 ctgcgggata gtttggcata gagcgcaagg tcggactggc gcgtttgctt aagcatgaac
     2161 tccttcagcg gggccgcacc tttttccatg gcgtcggcca tggagatttt gttttcagag
     2221 aaaggctggt aggcatccac gtaaatcctg tcgaataccg gacccatgac gaaaagtgtc
     2281 aggaacagcg cgaggccgag cagcacttgc gtaggcggag tcgagttcgt gcccagcgca
     2341 tggcgcagca gtgccagcac gatgatgatg cgggtaaagc tggtcatcat cagcaaggca
     2401 gccggaataa atgacaggga cgtcaggagc agcatcgtct ggatgctgag attgtaggtc
     2461 tgaccaccac cggctgtcgg cgtgctggtg aaggccggca acccttgtgc caccgcagcg
     2521 agcggcaggc acagcaggaa cgccggcagc agatgcagcc agcgatgcgg gagtgccgaa
     2581 gaagtgctag ctgccgtcat tacgtttatc gatagtatgt ttcagccagg aggtgaagtt
     2641 tgtcgacatg gttgatgtcg agacggcgac atgctcctga cgcggcatgg tggtcaaggt
     2701 attgacgcgt ccgggcgcga cgccgacgac aatccattgg tcggcaactt caatgaccat
     2761 cacgcgctcg cgcgtgccga cgttgatgcc accgatgatt tttgttattg cattgctgcc
     2821 ggcgcccggc gtcaatccgc tacgtttgaa gatccaggcg atgccggcca tcaatgccag
     2881 aacggcgatc aggcccagca gcatggtgac gagactgcca gtcgccgagg gcagagtggc
     2941 ggttgccggt gcttgctggt cagctgcctg agccacgccg gcgatgaacg acgctgacac
     3001 tgcgatgaag ctgcgatgac ggctgtaata agcgcgagcc tgtgcgagac ggtgaaaacg
     3061 cttcatctat tcagtttgcg aatgcgctct gccggcgtga tgatatcggt cagacggata
     3121 ccgaatttgt cgttcaccac caccacttcg ccttgtgcaa tcaggcagcc attgacgagt
     3181 acatccatcg gttcgccagc cataccaccg agttccacca ccgacccctg tgccagttgc
     3241 agcaggtttt tgatggcgat tttggtgcgc ccgagttcga ctgtcaactg caccgggata
     3301 tcgaggatga ggtcgatgtc gttcagtgca tcatttgcgc gctggccagt accaccgagt
     3361 tctttgaaaa cgcccgcctc agccgcttga gttgcctttg acgatttgcg cgcgggcttt
     3421 gctgcagaac cttctttttc cgccggtgcc ggttcggccg gcgcggcacc ctgcggatcg
     3481 acttttgccg ctgtttggtc aacgttctct tcagccattc tgctctcctt gaatactttc
     3541 cagtgtttcc tgttgcgcga tggacagcag tttctcgaca cgcaaggcgt attgaccgtt
     3601 caattggcca tagctgcatt ccatcacagg gatgccatcg acctgcgcga cgaccgattc
     3661 tccaggctgc aatgggatga catcgccgac ctgcatgcgc agcaagtcag taaatgtgag
     3721 ctgggcagta ccaaagttag cgatcagttc tacttcggca gcctggattt gctgcttcat
     3781 caaacggatc cagcgcttgt cgatttccag ggcttcaccc tgtatgctgc tggtcagctt
     3841 atcccggatt ggttcgatca tcgagtaagg caagcagata tgtatttcgc cgctgaccga
     3901 gccgagattc atggtgaaag tagtggagac tacaatttcg ttgggcgtgg cgatattggc
     3961 gaactgcgta ttgagttcgg aacggatgta ttcgaatttg atcggataga tcggctccca
     4021 ggatttagca tagttttcgg tgacgatatc gagtatacac atgatgatgc gttgctcggt
     4081 cggtgtgaat gcgcggcctt cgacacggat aggaaaac
//
LOCUS       DASRDH010000057         2185 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_84433,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000057 DASRDH010000000
VERSION     DASRDH010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84433"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1407)
                     /locus_tag="VFA14_01430"
     CDS             complement(<1..1407)
                     /locus_tag="VFA14_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HZS80086.1"
                     /translation="MHTVETVLLVLMLGAITGIVARYIRGIPLPLIQIGLGAAISWPQ
                     QGLHIAFDPELFLLLFIPPLLFADGWRIPKREFFALYQPILKLALGLALFTVIGLGYL
                     IHWMIPEMPLTVAFALAAVVSPTDAVAVSAITRNLGMPPQTMHVLEGESLLNDASGLV
                     ALKFAVAATLTGLFSWTEVAKDFLWMALGGLGVGALIGWGFAVARDRVTRRLGDVAAI
                     QMVLLLLLLPFAAYIVGERIGVSGILAAVAAGITTNFAELNRSKFIAERMQLQGTWAI
                     METAFNGAIFLLLGLQLPSIIGVTLQAAGHQWWIPVSYAAIISLALLLLRWIWLTVGV
                     QNSLWRAHREGKMVEKPSRLLTLVSTLAGIRGAVTLAGALSVPLLLNNGIAFPARDQL
                     IFLATGTILFTLLVGSIGLPLVLRCVPRSGVPPWAMEERHARLAACKAAIASMVLDKE
                     QVKSVDPQWLAQYQESAGL"
     gene            complement(1476..>2185)
                     /locus_tag="VFA14_01435"
     CDS             complement(1476..>2185)
                     /locus_tag="VFA14_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006465548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RQC domain-containing protein"
                     /protein_id="HZS80087.1"
                     /translation="CETLHCRRVRLLDYFGQQGQQPCGNCDICLTPPVSFDGTVMVQK
                     LLSTVYRVEQRFAPGHVLEVLRGIDSGKIRQWRHDSLSVFGIGSEQSEAEWRAVLRQV
                     IALDLLIVDHDNYSALKLTEAARPVLRGEQKIQLRQYQKPVKQKRASSGSSYVETSLT
                     TAEQAIFDKLRWWRVETARRHNVPAYVIFHDATMREIARARPASFDVLRGITGVGQRK
                     LDTYGAEIIALIQEMSA"
BASE COUNT          487 a          650 c          647 g          401 t
ORIGIN      
        1 caggccagcc gactcctgat actgcgcgag ccattgcgga tcgactgatt tcacctgctc
       61 cttatcgagc accatgctgg cgatcgccgc cttgcaggcc gccagcctgg cgtggcgttc
      121 ctccattgcc catggaggaa cgccgctgcg cggcacgcat cgcaaaacca gcggcagtcc
      181 aatgctgccg accagcaggg tgaagagaat ggtgccggtg gccagaaaga tcaattggtc
      241 gcgggccgga aaggcgatac cgttgttcaa cagcaacgga accgagagcg cgccagccag
      301 cgtcaccgcg ccgcgaatgc cggccagcgt gctgaccagg gtcagcaatc gtgaaggctt
      361 ttcgaccatc tttccttccc ggtgcgcgcg ccagagactg ttttgcacgc cgacggtgag
      421 ccagatccag cgcagcaata gcaaggccaa ggatatgatt gctgcatagc ttacggggat
      481 ccaccactgg tgacctgccg cctgcagcgt cacgccgatg atggaaggga gttgcaggcc
      541 cagcagcaga aagatggcgc cattgaaggc cgtctccatg atggcccagg ttccttgcaa
      601 ctgcatgcgc tcagcgatga acttgctgcg attgagttcg gcaaaattgg tcgtgatacc
      661 ggcggcgacc gccgccagaa tacccgagac gccaatccgt tcacccacga tgtaagccgc
      721 gaagggcagc agcaagagca gcagcaccat ctggatagcg gccacgtcgc cgagcctgcg
      781 tgtcactcgg tcgcgtgcca ctgcaaagcc ccagccgatc aaggcaccca cgccaagtcc
      841 gcccagcgcc atccatagga agtcttttgc tacctcggtc catgagaaca gtccggtgag
      901 ggtggccgcg acagcgaact tcagtgccac caaaccggac gcgtcattga gcagtgattc
      961 tccttcgagt acatgcatcg tctgcggggg catccccaga ttacgcgtga tggccgacac
     1021 cgccaccgca tcggtgggcg atactaccgc cgcgagcgcg aaagcaacgg tcaagggcat
     1081 ttcaggaatc atccaatgga tgagatagcc cagtccgatc acggtgaaca gcgccaagcc
     1141 gagtgccagc ttcaagatcg gctggtagag cgcgaagaat tcacgctttg gaattcgcca
     1201 accatcggcg aacagcaaag gtgggatgaa cagcagtagg aacagctcag gatcaaaggc
     1261 gatatgcaag ccttgctggg gccaggatat tgccgcgccc aggccgatct ggatcagagg
     1321 caagggaatc ccacgaatat agcgggcaac gatgccggtg atcgctccaa gcatcagcac
     1381 cagaagcacg gtttcaacgg tatgcatgtg taagttctca acattcagga tacatcgtaa
     1441 aaattcaaaa acaaagactg cctaaaagcc aatcgctacg cagacatttc ctgaatgagc
     1501 gcaatgatct cagcgccata ggtgtccagt tttctctggc cgacgccagt gatgccgcgt
     1561 aatacatcga acgacgccgg tctggccctg gcgatttcgc gcatggtggc gtcgtggaaa
     1621 atcacgtagg ccggtacatt gtgcctgcgc gcagtctcga cgcgccacca gcgcagtttg
     1681 tcgaagatgg cctgttctgc agtcgtcaga ctggtttcga cgtagctgga tccggacgag
     1741 gcgcgtttct gcttgaccgg cttctggtac tgacgcaact gaatcttttg ttcgccgcgc
     1801 agcaccggac gcgctgcttc ggtgagcttg agcgcactgt agttgtcgtg atcgacgatg
     1861 agcaggtcca gcgcaatgac ctgccgcaac acggcgcgcc attcggcttc gctctgttcg
     1921 ctgccgatgc cgaacaccga caggctatcg tggcgccatt gcctgatctt gccggaatcg
     1981 atgccgcgca ggacttccag tacgtgtccg ggggcgaagc gctgttcgac gcggtagacg
     2041 gtggacagta acttttgcac catcacggtc ccatcgaaag ataccggcgg tgtcagacag
     2101 atgtcgcagt tgccgcaggg ctgctggcct tgctggccga aataatcgag caggcgcaca
     2161 cgccggcaat gcagggtttc gcaca
//
LOCUS       DASRDH010000058         3906 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_537200,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000058 DASRDH010000000
VERSION     DASRDH010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 3906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3906
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_537200"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            178..759
                     /gene="flhC"
                     /locus_tag="VFA14_01440"
     CDS             178..759
                     /gene="flhC"
                     /locus_tag="VFA14_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HZS80088.1"
                     /translation="MAKKSVITEAQEILLAIELINLGARLQLLESETSLSRERLLKLY
                     KEIRGVSPPKGMLPFSTDWFITWQPNIHSSLFINIHKYLVEYAKISGIEAVMKAYKLY
                     LEQVNYVVEGDESVLSLTRAWTLVRFFESKMLTTMPCSKCNGHFVVHRLDLHQDYLCG
                     LCHMPSRAGKTKKAKDAIVAPLIDALPMVAAVA"
     gene            778..1605
                     /locus_tag="VFA14_01445"
     CDS             778..1605
                     /locus_tag="VFA14_01445"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZS80089.1"
                     /translation="MLLKTGDFKREKGLESGMESMQRRPFLQTPAIQALIILCITLIL
                     ALLAGIAITFAFGVDITPIPFAFLHGVLAAGIAYRHRMASWWVPILFLFPPIAVLVNT
                     LHLPPALFFVIFLFLLLLFWSTFRSQVPFYPSGRPVWDAVAALLPVEKPLQIIDIGSG
                     LGGAILYLSRVRKDCRFVGIELAPLPWLISHVRAVLSGSRGNFIRGDYTQLDFADYDV
                     IFAYLSPAAMTALWQKARSEMCAGTLLLSYEFIIAGVAADIVVQPQKNGPVLYGWHM"
     gene            1758..2618
                     /gene="motA"
                     /locus_tag="VFA14_01450"
     CDS             1758..2618
                     /gene="motA"
                     /locus_tag="VFA14_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HZS80090.1"
                     /translation="MLVIIGYVIVAGSVFGGFVLAGGHLTALLQPIELLMIGGAAAGA
                     FLVGNSGKAIKSILKALPSVLKGSSYSKALYMELMSLLFELLTKSRKEGLMSIEGDIE
                     GPETSPIFSKYPSVLADHHLIEFLTDYLRMMVSGNTDPLQIENLMDNEIETHHHEGEI
                     PVHAIAKLGDAMPAFGIVAAVMGVVHTMESVGIPPAELGMLIAHALVGTFLGILLAYG
                     FVGPLSSLLEQKLDESSKIYQCVKMTLLASLNGYAPALSIEFGRKVLFSTERPSFLEL
                     EAHVKQAKNK"
     gene            2653..3597
                     /gene="motB"
                     /locus_tag="VFA14_01455"
     CDS             2653..3597
                     /gene="motB"
                     /locus_tag="VFA14_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HZS80091.1"
                     /translation="MADEGLRPIIVKRIKKGGGGHHGGAWKIAYADFVTAMMAFFLLM
                     WLLSSTSKGELHGIAEYFRSPLKVAMTGGQSSGNSSVVLPGGGDDLVRQYGQRNSGES
                     TRNMRTRTTPPTPTEQEKKERIRFDRLKKRVETAINNNPTLQQFKNQLLIDVTTEGLR
                     IQVIDEKNRPMFALASAQLQPYTREILHEIGKTLNDVPNRISLSGHTDAKPYSSGEKG
                     YSNWELSTDRANASRRALVAGGMDESKILRVVGLSSSVLLDTKDPFNPSNRRISIIIM
                     NKKTEEAVMNNGPTMDVTSDAEIKKGLNVPEKNQDKPA"
     gene            complement(3650..>3906)
                     /locus_tag="VFA14_01460"
     CDS             complement(3650..>3906)
                     /locus_tag="VFA14_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2863 family protein"
                     /protein_id="HZS80092.1"
                     /translation="YGIVWPLYEQEDDEIHNLAGVKTPMEEMLEVLRECGIVHIKLHE
                     ERYAMEFCDDCGVPLFPAPDGDLVHAEMPENTSTSIEHFH"
BASE COUNT          965 a          975 c          985 g          981 t
ORIGIN      
        1 tgccgccatt ctcatggctt gccaaccaat cggataaatt tcctgattat atttctcgct
       61 ttcttcgtga tgtgccaagg ttgcaggagc gcagccaggg agaatgattc ggcgctgcca
      121 gtccaaagtc gaacgcatag atcggtcaac ttgtagtgaa aaatatcggg ggaatttatg
      181 gccaagaaaa gcgtgattac agaggcacaa gaaattctgt tagccattga gctgatcaat
      241 ctaggagcgc gtctccaatt actggaatca gaaacatcct tgtcacgcga acgcctgctc
      301 aagctttata aagagatcag aggcgtttca ccacctaaag gtatgctgcc tttttcgaca
      361 gattggttca tcacctggca acccaatatt cattcttcct tgttcatcaa cattcataaa
      421 tatctggtcg aatatgcaaa aatttccggt atagaagccg tcatgaaggc ttataaactc
      481 tacctggagc aggtcaacta cgttgtcgaa ggcgatgagt cggtcttgtc actgacccgt
      541 gcctggacct tggtacgttt cttcgagagc aaaatgctaa cgactatgcc ttgcagcaag
      601 tgcaatggtc attttgtcgt gcatcgcctg gacttgcacc aggattatct ctgcggccta
      661 tgccacatgc cttcccgtgc gggcaaaacc aagaaggcca aggatgccat cgtagcgcca
      721 ttgattgacg ccttgccgat ggtcgctgct gtcgcctaag ttcagtttct ctctacgttg
      781 ttattaaaaa caggcgactt taagagagag aaaggcttgg agagtggtat ggagtcgatg
      841 caacgcagac cttttctgca gacacccgct atacaggctc tgatcatttt atgcattacc
      901 ctgatattag cgctgcttgc cgggatagcg atcacattcg ccttcggggt cgacataact
      961 cccatccctt tcgcttttct gcatggtgta ctggcagcag gtatcgctta tcgacatcgc
     1021 atggcatcgt ggtgggtgcc gattcttttt ctctttcccc cgattgcagt actggtcaac
     1081 acgctgcatt tgccgccggc gctgtttttt gtcattttcc tgtttttact gttgttgttc
     1141 tggtcgacat ttcgcagcca ggtgccgttc tatccatctg gacgccctgt ctgggatgcc
     1201 gtagctgcgc tgttaccagt cgaaaagccg ttgcagatca ttgatatcgg cagcggcctt
     1261 ggcggcgcga tactgtattt gtcgcgcgtg cgcaaggact gccgttttgt cggcatcgaa
     1321 ctagcaccgc tgccctggct gataagtcat gttcgtgcag tgctaagcgg cagtcgcggc
     1381 aattttatcc gtggcgacta tacacaactg gattttgccg attatgacgt gatcttcgcc
     1441 tatctctccc cagcggcgat gaccgcgttg tggcaaaaag cacgctccga aatgtgtgcc
     1501 ggtacgctgt tgttgagtta tgagttcatc attgcagggg tcgcggcgga tattgttgta
     1561 cagccccaaa aaaacggccc ggttttatat ggttggcata tgtgaaacaa aaagagccgt
     1621 gttacgttca tctcacatgt cgtaattgaa aatgaaagcc ctctttcagt catgtattcc
     1681 atcgtttgtt tgaaaaaatt gtatgtaacc tgataactgg taaaaacact gaacgtcact
     1741 gaggcaggag caagcacttg ttagtcatca ttggatatgt gatcgtcgca gggtccgttt
     1801 ttggcggatt cgtcctagcc ggggggcacc tcacggcctt gcttcagcct atcgaattac
     1861 tgatgatcgg tggtgctgca gctggcgctt ttctggtcgg caatagtggc aaagcgatca
     1921 agagcatact aaaagcgcta ccgtcagtac taaagggatc gtcctacagc aaggctcttt
     1981 acatggaatt gatgtcctta ttgttcgaac ttctcaccaa atcacgtaaa gaaggcctga
     2041 tgtcgatcga aggcgatatc gaaggacctg aaaccagtcc aattttcagc aaatatccca
     2101 gtgttctcgc cgaccatcac ctgatcgaat ttctgaccga ctacctgcgc atgatggtgt
     2161 cggggaacac ggatccgctc cagatcgaga atttgatgga taacgaaatc gaaacacatc
     2221 atcacgaggg ggagattcca gtacatgcta ttgccaagct aggtgacgcc atgccggcgt
     2281 tcggtatcgt cgcggcggtg atgggcgtgg tgcatacgat ggagtcggtg ggtattccac
     2341 ctgctgagct aggtatgctg attgcgcacg ctttggtcgg caccttcctc ggcattctgt
     2401 tggcctatgg ttttgtaggg ccgttgtcgt ccttgctgga acaaaaactc gacgaatcgt
     2461 ccaaaatcta tcagtgcgtc aagatgacct tgcttgccag tctgaacggt tacgcacctg
     2521 ctttgtcgat cgagttcggc cgcaaggttc tgttttcgac tgaacgccca tccttcctgg
     2581 agctggaagc acatgtcaag caggcaaaga acaagtaacc cgataccgat aagttattcg
     2641 caggagtaag gcatggccga cgaagggcta cgtcccatta ttgtcaagcg catcaaaaaa
     2701 ggtgggggtg gtcatcacgg cggcgcctgg aaaatcgcct acgccgactt cgtcacggcg
     2761 atgatggcgt tcttcctgct gatgtggttg ctcagttcga cttccaaagg agaacttcac
     2821 ggtatcgccg aatatttcag atcccccctc aaggtagcga tgactggcgg ccagagcagc
     2881 ggtaactcca gtgtggtgtt gcctggcggc ggcgatgatc tggtgcgtca gtatggtcag
     2941 agaaacagcg gtgagagtac acgcaatatg cgtactcgta ctaccccgcc gactccaaca
     3001 gaacaggaaa aaaaggaacg tatacgtttt gaccgcctga aaaagcgcgt cgaaaccgcc
     3061 attaataata atccaacgct ccagcaattc aaaaatcaac tgctgatcga cgtcaccacg
     3121 gaaggtttgc gcatccaggt tatcgatgag aaaaaccgcc cgatgtttgc gttagccagt
     3181 gcgcaattgc aaccctacac acgcgaaatc ctgcatgaaa tcggcaagac actcaatgat
     3241 gtgccaaaca ggatcagtct gtccggtcat accgatgcga agccgtattc cagcggtgaa
     3301 aagggataca gcaattggga actatcgacc gaccgcgcca acgcatcgcg ccgtgcccta
     3361 gttgccggcg gcatggatga gtcaaaaata ctgcgcgtgg tgggcttgtc ctcatcggtg
     3421 ctgctcgata caaaagatcc gtttaatccg agcaaccgtc gcatcagtat catcatcatg
     3481 aataagaaaa cagaggaagc agtgatgaat aacggaccga cgatggatgt cacgtctgat
     3541 gccgaaatca agaaaggcct gaacgtacca gaaaaaaatc aagacaagcc tgcataagcg
     3601 gacactcgat cgtcctatag gaaaagctgg cggcttgaca gaatagtggt tagtgaaagt
     3661 gttctatcga agtcgatgta ttttctggca tttccgcatg caccagatca ccatcaggtg
     3721 ctggaaacaa aggcacgccg caatcgtcgc aaaattccat cgcatagcgt tcttcatgca
     3781 gcttgatatg cacaatcccg cattcgcgca aaacctccag catttcttcc atcggtgttt
     3841 tgacgccagc cagattatga atctcatcgt cttcctgctc gtatagcggc cacacaatgc
     3901 cataga
//
LOCUS       DASRDH010000059         2745 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_534252,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000059 DASRDH010000000
VERSION     DASRDH010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_534252"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="VFA14_01465"
     CDS             <1..218
                     /locus_tag="VFA14_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012734415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZS80093.1"
                     /translation="MQGGVDVWNDIANGVLKSVVFGIAVTFVALFQGYEARPTPEGVA
                     RATTRTVVIASLLVLALDFLLTALMFS"
     gene            280..756
                     /gene="mlaD"
                     /locus_tag="VFA14_01470"
     CDS             280..756
                     /gene="mlaD"
                     /locus_tag="VFA14_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HZS80094.1"
                     /translation="MQRKTLDVWVGGFVLLGAAALVFLALKAGNMNSFSFSQKTYTVM
                     TKFDNIGGLKPRAAVRSAGVVVGRVAAISFDDKAFQAVVTLDMDERYQFPKDSSVKIL
                     TSGLLGEQYIGIEPGGDAINLAAGDMIRMTQSAIVLENLISQFLYSKAADGKGDSQ"
     gene            753..1463
                     /locus_tag="VFA14_01475"
     CDS             753..1463
                     /locus_tag="VFA14_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007879609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HZS80095.1"
                     /translation="MKYIARIGALVMVVALSGCATSNNPSDPLEGFNRAMFSFNDAVD
                     KVALKPTAQAYQFALPDFVQAGIGNFFGNIGDLWSAVNQLLQGRVETGVTSFMRVAVN
                     TTFGLGGVLDVSTEARLPKQQSDFGQTLGIWGVGSGPYLVLPLLGPSMVRDTAALPAD
                     FYGNLWSYKKPVRWRNAGAGIHIVDRRAQLLEAGSLLEDVALDKYDFVRDTYLQSRQA
                     RIDDLKGKEPAREKVVEP"
     gene            1509..2144
                     /locus_tag="VFA14_01480"
     CDS             1509..2144
                     /locus_tag="VFA14_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZS80096.1"
                     /translation="MNILTKLLSMTLSVFALTFASAVAAAEAPDALVKRISEEVLNTA
                     KADKSIQAGNISSIVALVEEKILPYVDFERMTALAAGRYWRQATPEQQKQLLTEFRSL
                     LIYTYSGALSQVRDQKIEYKPLRADPANTEVEVRSQVIQSRGEPIQLSYRLEKLATGW
                     KLYDINVMGAWLVETYRGTFNSEITKGGIDGLIKSLSDKNRQLAARSAAKK"
     gene            2166..2459
                     /locus_tag="VFA14_01485"
     CDS             2166..2459
                     /locus_tag="VFA14_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008332479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HZS80097.1"
                     /translation="MFKPGASLTVDNAVATLTNGLQAIAAGQAAIDLSAITTVDSSAV
                     AILLSWQRAARVQGLALQFSALPPNLQSLVDLYGAASLLDAPASVSDQHCSAG"
     gene            complement(2567..2689)
                     /locus_tag="VFA14_01490"
                     /pseudo
     CDS             complement(2567..2689)
                     /locus_tag="VFA14_01490"
                     /inference="COORDINATES: protein motif:HMM:NF015377.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
BASE COUNT          579 a          708 c          838 g          620 t
ORIGIN      
        1 agatgcaggg tggcgtggat gtctggaatg acatcgccaa cggcgtgctc aaaagcgtgg
       61 tgttcggtat cgcggtcacc tttgtcgccc tgtttcaggg atatgaagcc aggccgacgc
      121 cggagggcgt cgcgcgggcg acgacgcgca ccgtcgtgat cgcgtcgttg ctggtgctgg
      181 cattggattt tctgctgact gcgctgatgt tcagttgaac ggcgcgacgg tcggcaattg
      241 taaaggcagg tcttcgattg atttttggga tggcaaggta tgcaacgtaa aacattggac
      301 gtgtgggtag gcgggtttgt cctgctgggc gcggcggccc tggtttttct ggcgctgaag
      361 gccggcaaca tgaattcgtt ctctttttcc cagaagactt acaccgtcat gaccaaattc
      421 gataacatcg gcggcctgaa gccgcgcgcg gcggtcagga gcgccggcgt ggtggtgggg
      481 cgcgtcgctg caatcagctt cgatgacaag gcattccagg ctgtggtgac gcttgacatg
      541 gacgaacgct atcagtttcc caaggatagc tccgtcaaaa tattgacttc gggcttgctg
      601 ggcgaacagt acatcggtat tgagccgggg ggggatgcaa taaatctggc ggcgggtgac
      661 atgatcagga tgacgcaatc ggcgatcgtt ctggaaaacc tgatcagcca gtttttatat
      721 agtaaggcag cagatggaaa aggtgattca caatgaaata catcgctcgt attggtgcgc
      781 tggtcatggt tgtcgcgctc agcggttgcg caacctcgaa taacccgagt gatccgctgg
      841 agggtttcaa ccgtgccatg ttcagtttca acgatgcggt cgacaaggtg gcgttgaagc
      901 cgacagcgca ggcttatcaa tttgccctgc ctgatttcgt gcaggcaggc atcggcaact
      961 ttttcggcaa tattggtgac ttgtggtcag cggtgaacca gcttttgcaa ggccgcgtcg
     1021 aaaccggcgt cactagtttc atgcgcgtgg cagtcaatac cacgttcggt ctgggcggcg
     1081 tgctcgatgt cagtactgag gcgcgtttgc cgaaacagca aagcgacttt ggccagactc
     1141 tgggcatatg gggcgtaggt tccggtcctt atctggtgct gccgttgctg ggaccgtcca
     1201 tggtgcgtga taccgcagcc ctgccggccg atttctacgg caatctgtgg agctacaaga
     1261 agcctgtgcg ctggcgtaac gctggtgcgg gaattcatat cgttgacagg cgcgcgcaac
     1321 tgctggaggc aggcagtctg ctggaagacg ttgcgctgga taaatacgat ttcgtgcgcg
     1381 atacttatct gcaaagccgt caggccagga tcgatgatct caagggtaag gaaccggcca
     1441 gggaaaaagt ggtcgagccg taagaagaac gtgcgattgc aacgcttatt tttaaatgga
     1501 tcggaaaaat gaacatactg acgaaactac tatcgatgac cttgtccgtt ttcgcactga
     1561 ctttcgcttc tgctgttgcc gcggcagaag cgccggatgc actggtcaag cgtatcagcg
     1621 aagaggtgct gaacacggcc aaggcagaca agtccatcca ggctggcaac atcagtagca
     1681 tcgtcgcgct ggtggaagaa aaaatcctgc cctatgtcga tttcgagcgc atgaccgcgc
     1741 tggctgctgg ccgttactgg cgccaggcga cgccggaaca gcaaaagcag ctgctcactg
     1801 aatttcgcag cctgttgatt tacacctact ccggcgcgct gtcgcaagtg cgtgaccaga
     1861 agatcgaata caagcccttg cgcgccgatc cggcgaatac tgaagtggaa gttcgttcgc
     1921 aagtgatcca gtcacgtggt gaaccgatcc agttgagcta tcgtttggaa aaactggcga
     1981 ccggctggaa gctgtatgac atcaatgtca tgggcgcatg gttggtggaa acctacaggg
     2041 ggactttcaa cagcgaaatt accaaaggcg gcatcgatgg tctgatcaag agcctgtccg
     2101 acaaaaacag gcagcttgcg gcgcgttccg ctgctaaaaa ataagcgcga cacaggaagt
     2161 ccgccatgtt caagccgggt gcctctttga ccgtcgataa cgctgttgcc acactgacga
     2221 atggtttgca ggcgatcgct gccggtcagg cagcgatcga cctgagtgcg atcacgacgg
     2281 tggattcttc tgcggtggcg attctgctgt cctggcagcg tgctgccaga gtgcagggcc
     2341 tggccttgca gttcagcgcg ctgccgccca atctgcaaag tctggtcgat ctgtacggcg
     2401 ccgcttccct gctggacgcg ccggcttctg tttctgacca gcattgttca gccgggtgat
     2461 ccttgcgtga ctggttagtg tcttcgccaa atcgtacata cccagtgatc atttgcggat
     2521 aaattcttcg cacaagctgt tcccaactcg cggcgaagcc ctgcgcccga aaaccaaact
     2581 gcggcagggc gcgcgccatg tcctcaaagc tggcccgtcc ttcgctcttg ttcatggcgt
     2641 ttagcaaggc ctcctcggtg atgctccggc catagtagct gccgagcaaa gtggccaggg
     2701 acgctgcccg caggaatagt ccagattctg tttgacaatg cgccc
//
LOCUS       DASRDH010000060         3659 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_192350,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000060 DASRDH010000000
VERSION     DASRDH010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 3659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3659
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192350"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1741)
                     /locus_tag="VFA14_01495"
     CDS             complement(<1..1741)
                     /locus_tag="VFA14_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016836382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HZS80098.1"
                     /translation="MPFPCFALLDDNTAGAPSAHARSRLYTAYHATLTCHDGKALPAT
                     LRRMQAALQQGLHAVALCSYELGADLLGITPRVETGMALVEILLFSQCRHMNAAETAA
                     WLQEQECRERAGHDSPSGIANVRASVDEARFTRDIARIRDDIAAGDTYQVNYTYRLYF
                     DAYGAPAALYNKLRARQPVPYGAFIRLPDARCILSFSPELFVRHTQGCLHAQPMKGTA
                     PASSDAQENARRAAALSHDEKNRAENLMIVDLLRNDLGQIAINGSVAVPHLFAVETFG
                     QVLQMTSSVTARLRPDASLAEVFGALYPCGSITGAPKRRTMQIIRALENTPRGLYTGA
                     IGWFDAAPPNRAVGDFCLSVPIRALILQPQQHGLRRGEMGVGAGIVHDSHAAGEYAEC
                     RLKAGFLTGPDSDGSDFSLFETLHATQADGCRYLDRHLQRLQGSATYFGFACDIAAIR
                     TLFTAACAQLPPVIPHRLRLDLSRDGTLRLQHSALSPLPERSERIKVFVTAAVMDSDD
                     LFLRHKSSVRARYDAAWKTAEQHGGFDMLFFNQRGELTEGGRSNVFVQLEGRWYTPPL
                     AAGVLPGVMRAVLL"
     gene            1875..2144
                     /locus_tag="VFA14_01500"
     CDS             1875..2144
                     /locus_tag="VFA14_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS80099.1"
                     /translation="MKRFIALPLSVCIVFILGACEQKIKTAAENAERDAKFEQAERNA
                     EERRVKNNEEERLLLEKAEEERRLRKAEEERLAREKAEKEAPRSR"
     gene            2235..2549
                     /locus_tag="VFA14_01505"
     CDS             2235..2549
                     /locus_tag="VFA14_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS80100.1"
                     /translation="MKRFIALSLSACIVFTACKPDEFEKKAERDAAAQQAINEDERFQ
                     KETERDAAAQQAVNDEARRQKQAERGAEEQGLNNDELEKKAERDAAAQQAINEDPGGQ
                     KK"
     gene            2847..>3659
                     /locus_tag="VFA14_01510"
     CDS             2847..>3659
                     /locus_tag="VFA14_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZS80101.1"
                     /translation="MVKPQHRIFLDVQDPLLLDLQLSDDERQVRDAAHAYCQARLAPR
                     ILSAFRHETTDTAIFREMGELGLLGVMIPEACGGAGLNHVSYGLVAREVERVDSSYRS
                     MMSVQSSLVMLPINQFGTAAQRQKYLPKLACGEWIGCFGLTEPDHGSDPGGMLTRARK
                     VAGGYCLSGSKMWITNSPIADVFVVWARDDAGDIRGFILDKGAAGLSTPVIHGKLGLR
                     ASITGGIVMDEVFCPEENAFPEVRGLKGPFACLDSARYGIAWGALGAAEDCFE"
BASE COUNT          754 a          994 c         1173 g          738 t
ORIGIN      
        1 ccagcagcac cgcgcgcatc acgccgggca ggacgccggc ggccagcggc ggcgtgtacc
       61 agcgaccttc cagttgcacg aagacgttgc tgcgtccgcc ttcggtgagt tcgccgcgct
      121 gattgaagaa cagcatgtcg aagccaccgt gctgctcagc agttttccag gcagcgtcat
      181 agcgtgcgcg caccgaactc ttgtgacgca ggaacagatc atcgctgtcc atgaccgcgg
      241 cggtgacaaa cactttgatg cgctcggatc gctcaggtaa cggcgacaga gcgctatgct
      301 gcaagcgcag cgtaccatca cgcgacaggt cgagccgcag gcgatgcggt attaccggcg
      361 gcagttgcgc gcaggcggcg gtaaatagcg tgcgtatggc cgctatgtcg caggcgaaac
      421 cgaagtacgt agctgagcct tgcagccgtt gcagatgccg gtcgaggtag cggcagccgt
      481 ctgcctgcgt cgcatgcagg gtttcaaaca ggctaaagtc actgccgtcg ctatcgggtc
      541 cggtcaaaaa gcctgctttc agacggcatt cggcatattc gccggcggca tggctgtcat
      601 ggacgatgcc ggcaccgacg cccatttctc cacgccgcag gccatgttgc tgcggttgca
      661 gtatgagcgc acggattggc accgacaggc agaaatcgcc tacggctctg ttgggcggcg
      721 cggcatcgaa ccagccgatg gcgccggtgt agaggccgcg cggcgtgttt tccagcgcgc
      781 ggatgatttg catggtgcgt cgtttgggcg cgccggtgat agaaccgcaa ggataaagcg
      841 cgccgaagac ctcggcgaga ctggcgtcgg ggcgcaaccg ggcggtgacg ctggaagtca
      901 tctgcaatac ctggccgaaa gtttcgactg cgaacaggtg cggcaccgcg accgaaccgt
      961 tgatggcgat ttgccccaga tcgttgcgca gcagatcgac gatcatcagg ttttcggcgc
     1021 ggtttttttc atcgtgcgat agggcagcgg cgcggcgggc gttttcctgc gcatcgctgg
     1081 aggccggcgc agtgcctttc attggttgcg catgcaggca tccttgcgtg tggcgcacga
     1141 acagttccgg cgaaaacgac aggatgcagc gtgcgtcggg caagcgtatg aacgcaccat
     1201 acggcactgg ctggcgcgcg cgcaatttgt tgtacaaggc tgccggagcg ccgtaggcgt
     1261 cgaaatacag gcggtaggtg tagttgacct gataggtgtc gccggcggca atgtcatcgc
     1321 ggatacgggc gatatcacgg gtaaaccgtg cctcatcgac gctggcgcgc acattggcga
     1381 taccgctcgg gctgtcatgc cctgcgcgct cccggcattc ctgttcttgc aaccaggcgg
     1441 cggtttccgc tgcgttcata tgacggcatt gcgaaaacag caggatttcc accaacgcca
     1501 tgccggtttc gacgcgcggc gtgatcccga gcaagtcggc gcccagttca taactgcata
     1561 gcgccacggc atgcaggccc tgctgcagcg ctgcctgcat gcgcctgagc gtggccggta
     1621 gcgccttgcc gtcatggcag gtcagtgtgg cgtggtacgc cgtgtacagg cgcgaacgcg
     1681 catgggcgga tggcgcgccg gcagtgttgt catcgagcaa ggcaaagcag gggaacggca
     1741 tcatgggacg aaaatgcact gcgggttcat aaggggacgc tcatggagcc tgtccggcag
     1801 catcataact tgactttgtt tctggcagag cttgagttag actttggtct gagaaaagac
     1861 tttaggagaa tgccatgaag cgtttcatcg ccttgccgtt gtctgtctgt atcgttttca
     1921 tcctgggtgc ttgtgaacaa aaaataaaaa cggcggctga aaacgcagag cgtgatgcca
     1981 aattcgaaca agcagagcgt aacgccgaag agcgacgcgt caagaacaac gaagaagagc
     2041 gtctcctcct cgagaaagcc gaagaagagc gccgcctcag aaaagccgaa gaagagcgtc
     2101 tcgcccgcga aaaagctgaa aaagaggcgc cgcgctctcg ataaccggtg agtaccgcgc
     2161 actcgtcgcc tggcctggcg tctggcaggg gcgggggtgg gttctgttct gaaaagagac
     2221 tttaggaaaa tgtcatgaag cgtttcatcg ccctgtcgtt gtctgcctgt atcgttttca
     2281 ctgcctgtaa accagatgaa ttcgaaaaaa aagcagaacg tgacgccgca gcgcaacagg
     2341 ccatcaatga ggacgagaga ttccagaaag aaacggaacg tgacgccgca gcgcaacagg
     2401 ccgtcaacga cgaggcgagg cgccagaaac aagcagaacg tggtgctgag gagcaaggtc
     2461 tcaacaacga tgagctcgaa aaaaaagcag aacgtgacgc cgcagcgcaa caagccatca
     2521 acgaggatcc ggggggccag aaaaaatagg agtctgcgcg gcgttactca ctgtgtgtgt
     2581 ttgcaggagc catcatgcct ttgttcagca caatttccgt tgtcccggga aggatttaca
     2641 ggcttgtgta ggtactgtgg ttgatgcggc gttcatcgaa catcggctga tcggtgcggt
     2701 gatattcgct tgtcggcacg atgatttgct tgtgggttcg tcggccgctc ttgtggtctg
     2761 agcgtcgtgg cgttgacgaa taccttgtac gaagcttgct tgggtcgctt cgtcacagac
     2821 ctgcgcgacg tcatctatcg gccatcatgg taaaaccgca acatcgcatt ttcctcgatg
     2881 tccaggatcc cttactgctc gacctgcaat tgagcgatga tgaacgtcag gtgcgtgacg
     2941 cggcgcatgc ctattgccag gctcgtcttg cgccacggat tctgtcggct ttccgccatg
     3001 aaaccacgga tactgccatc ttccgcgaaa tgggtgagct tggtctgctt ggcgtgatga
     3061 ttcctgaagc ctgcggcggc gccggactga accatgtcag ctatggtctg gtggcgcgtg
     3121 aagtagagcg cgtggattcc agctaccgct cgatgatgag cgtgcaatcg tcgctggtca
     3181 tgctgccgat caatcaattc ggtacggcgg cgcagcggca gaaatacctg ccgaagctcg
     3241 cttgtggcga atggataggt tgcttcggcc tgaccgagcc tgatcatgga tcggatcctg
     3301 gcggcatgct tacccgcgcc agaaaagtcg ccggcggcta ctgtctgagc ggcagcaaga
     3361 tgtggattac caatagtcct atcgccgatg tgttcgtggt ctgggccagg gacgatgctg
     3421 gtgacattcg cggcttcatt ctcgacaaag gggccgcagg cctgtcgacg ccggtgattc
     3481 acggcaagct tggtttacgt gcatcgatca ccggtgggat cgtgatggac gaggtatttt
     3541 gtccggaaga gaatgccttt cccgaggtgc gcggtctcaa agggccgttc gcctgcctcg
     3601 acagcgcgcg ctatggtatt gcctggggtg cgcttggtgc ggcggaagac tgcttcgag
//
LOCUS       DASRDH010000061         2850 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_786791,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000061 DASRDH010000000
VERSION     DASRDH010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2850
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_786791"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="VFA14_01515"
     CDS             <1..439
                     /locus_tag="VFA14_01515"
                     /inference="COORDINATES: protein motif:HMM:NF018925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1440 domain-containing protein"
                     /protein_id="HZS80102.1"
                     /translation="PGAHIDAWLSWMGFNSHSMDYVYQGAAIPGAITLYHWLFSFALA
                     FVYVVGSIYWPRIRLWYGALFGVIVTVAAHGFMIPLLGFRNPVYAHGATGWLWNLNGY
                     ELWSEFVGHIYWGVSIEVSMIAVLAYFARPIRGIWATPASAGG"
     gene            complement(519..923)
                     /gene="gloA"
                     /locus_tag="VFA14_01520"
     CDS             complement(519..923)
                     /gene="gloA"
                     /locus_tag="VFA14_01520"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004462 - lactoylglutathione lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HZS80103.1"
                     /translation="MRILHTMLRVGDLQRSIDFYTRVLGMKLLRTSENTEYQYTLAFL
                     GYGSNPDHAELELTYNHGVDKYEMGTAYGHIAISVEDAHKACEAVKAAGGNVAREAGP
                     VKGGCTVIAFVTDPDGYKVEFIEHKISPMMRG"
     gene            complement(1010..1085)
                     /locus_tag="VFA14_01525"
     tRNA            complement(1010..1085)
                     /locus_tag="VFA14_01525"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1050..1052),aa:His,seq:gtg)
     gene            complement(1170..2039)
                     /gene="murI"
                     /locus_tag="VFA14_01530"
     CDS             complement(1170..2039)
                     /gene="murI"
                     /locus_tag="VFA14_01530"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007879973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HZS80104.1"
                     /translation="MSELAAPAGSMPMDAGAPIGIFDSGIGGLSVLRHIYATLPQEHL
                     LYFADSAHAPYGDRDEQQIVERSLAIAAFLIEEGVKALVVACNTATAAAIQAIRRHWP
                     RHIVVGIEPGLKPAAQQSRSGIIGVLATRSTLTSARFIALCEQIAAAGKVSFMTQACV
                     GLADQIEKGELHSPATAMLVDRYVTPLVARGADTLVLGCTHYPFVRPLIEASARHAAC
                     ATPVVIDTGEAVTRQLIRLLDTGALRRQAGRGAGSLVAYTTADSGALSAAFASLLNMK
                     VAVRQIAVKCVPM"
     gene            complement(2071..>2850)
                     /locus_tag="VFA14_01535"
     CDS             complement(2071..>2850)
                     /locus_tag="VFA14_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005668663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FumA C-terminus/TtdB family hydratase beta
                     subunit"
                     /protein_id="HZS80105.1"
                     /translation="LDVKIMMYPTHAASKPVAMIPNCAATRHAHFVLDGAGPAYIEPP
                     SLSEWPEVHWAPDTEKSRRVNLDTLTREEVASWEPGQTLLLNGKMLTGRDAAHKRIQE
                     MLTRGEKLPVDFTSRVIYYVGPVDPVRDEVVGPAGPTTATRMDKFTDMMLEQTGLIAM
                     IGKAERGPVAIESIKRHGSAYLIAVGGAAYLVSKAIKSARVLGFADLGMEAIYEFDVK
                     DMPVTVAVDANGVSVHTTGPKEWQEKIAAGGNLGGIPVTTA"
BASE COUNT          563 a          842 c          807 g          638 t
ORIGIN      
        1 cccaggcgcg catatcgatg catggctgag ctggatggga ttcaattcac actccatgga
       61 ttatgtctat cagggagccg ctattccagg tgcgatcacg ctctatcact ggttgttcag
      121 ttttgccttg gcctttgtgt acgttgtcgg atccatctat tggcccagga tcaggctttg
      181 gtatggcgcc ttgtttggtg tgatcgttac ggtcgccgca catgggttta tgattccctt
      241 gctgggcttc agaaacccgg tctatgcgca tggcgcgaca ggctggctgt ggaatctgaa
      301 cggctatgaa ttatggagtg aattcgttgg acacatttac tggggtgtct ccattgaagt
      361 atccatgatt gccgttcttg cttacttcgc caggccaatc agggggatat gggccacccc
      421 ggcatcggca gggggttgat ccaaggtgga atgcacgagg gcttcgccgc gctgttggcg
      481 cggccctcgg ccaatatcgc cagtgtgctt ctttcccttc agccgcgcat catcggagat
      541 attttgtgtt cgatgaattc gactttatag ccatccgggt cagtcacgaa cgcaatcacc
      601 gtgcatcctc ccttcactgg gccggcttcg cgcgcgacat tgccaccggc ggccttgacg
      661 gcctcgcaag ccttgtgtgc gtcttcgacc gaaatggcga tatgaccata tgccgtaccc
      721 atttcgtact tgtcaacgcc atggttgtag gtcaattcca gttctgcgtg atcaggattg
      781 gaaccataac ccaggaatgc cagcgtatac tggtactccg tattttcaga agtacgcaac
      841 agtttcatcc cgagtacacg agtataaaaa tcgatggagc gctgcagatc gccaacgcgg
      901 agcatagtgt ggagaatgcg cattattttc ctcgatggtt gctgggtaaa agagcgtgag
      961 gttccgtggg gcggaacctg tatgcaaccc tggaagtgcc ttttgaacct ggggtggctg
     1021 acgggactcg aacccgcgac aacaggaatc acaatcctgg actctaccaa ctgagctaca
     1081 gccaccactg tattgaacag tgaagcgcaa gattatataa aaccgcagca gttctggcaa
     1141 atgaaaatag tcaccggcat gaaatgcgtt tacatcggca cacatttcac ggcgatctga
     1201 cgcactgcca ccttcatgtt caacagactg gcgaatgccg ccgacaaagc gccgctgtcg
     1261 gcggtcgtat aggccaccag acttcccgcg ccgcgccctg cctgtcgacg cagcgcccca
     1321 gtgtccagca ggcggatcaa ctggcgcgtt accgcctcgc cggtatcgat gaccactggc
     1381 gtggcgcaag cggcatgccg cgctgacgcc tcgatcagag ggcgcacaaa cggatagtgc
     1441 gtgcatccca gcaccagcgt atcggcgccc cgcgcgacca gtggcgtgac atagcggtct
     1501 accagcatgg ccgttgccgg cgaatgcaat tctccttttt cgatctgatc agccagaccg
     1561 acacaggcct gtgtcatgaa gctgaccttg cctgctgctg caatctgttc gcataatgca
     1621 atgaaacgcg cgctggtcaa tgtgctgcgc gtagccaata caccgatgat cccgctcctg
     1681 ctttgctgcg cggcgggttt caggccgggt tcgataccga ccacgatgtg ccttggccag
     1741 tgacgccgga tcgcctggat cgctgccgcg gtcgcggtat tgcaagcaac caccaatgcc
     1801 ttgacgccct cttcgatcag gaaggcggca atcgccagtg agcgctcgac gatctgctgc
     1861 tcgtccctgt cgccgtaagg cgcatgcgct gaatcggcga aatacagtaa atgctcctgc
     1921 ggcagcgttg cgtatatgtg acgcagcacc gacaatccgc caatgccgga atcgaaaatg
     1981 ccgatgggcg cgccggcgtc catcggcatc gatcctgccg gcgctgctag ttcactcatc
     2041 ggcgcacaag atatggctgg cgtggcgcca tcatgctgtc gtgacaggaa tcccgcccag
     2101 gttgccgccg gcggcgatct tttcctgcca ttccttcggc cctgtcgtat gcaccgatac
     2161 gccattggca tcgaccgcaa ccgtcaccgg catgtccttg acgtcgaatt cgtagattgc
     2221 ctccatgccc agatccgcaa aacccagcac cctggctgat ttgatcgctt tggacaccag
     2281 gtaagcagcg ccgccgacgg cgatcagata ggccgatccg tgccgcttga tcgattcgat
     2341 ggcgaccggg ccgcgttccg ccttgccaat catggcgatc aggccagtct gctccagcat
     2401 catgtcagtg aacttgtcca tgcgtgtcgc ggtggtcggc ccggcgggac cgaccacttc
     2461 gtcgcgtacc ggatcaaccg ggccgacata gtaaatcacg cggctggtga aatcgaccgg
     2521 cagcttttca cccctggtca gcatctcctg gatgcgcttg tgcgcggcat cacgtccggt
     2581 cagcatcttc ccgttcagca gcagggtctg accaggttcc catgatgcga cttcttccct
     2641 ggtcagggta tcgaggttga cgcgcctgga tttttcagtg tcaggcgccc aatgcacttc
     2701 cggccattcg gacagcgatg gcggttctat ataggccggg ccggcaccat ccagcacaaa
     2761 gtgagcgtga cgggtggcgg cgcagttcgg aatcatcgca accggtttcg atgccgcgtg
     2821 cgtcggatac atcatgatct tgacgtcgag
//
LOCUS       DASRDH010000062         5212 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1339318,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000062 DASRDH010000000
VERSION     DASRDH010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 5212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5212
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1339318"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(164..1186)
                     /locus_tag="VFA14_01540"
     CDS             complement(164..1186)
                     /locus_tag="VFA14_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HZS80106.1"
                     /translation="MPSPDSDQRLSLLQTWLSTLATTPTHIETLRPASADASFRRYFR
                     VDTPDGKTRIVMDAPPPQEDVRPFIHVAEVFGTAGMSVPTVLAQDVERGFLLLSDLGS
                     TTYLHLLDKDSAHQLYLDAIDALLLLQTRSRPGVLPEYGRALLLRELQLFPDWYLTRH
                     LEVTLNDDQKNTLANIFDTLLGNNLTQARVYVHRDYHSRNLMVLPDSNPGILDFQDAV
                     YGPITYDLASLLRDAYIRWDEEMVLDWAIRYWERARRAGLPVAPDIDAFYRDFECMGV
                     QRHLKILGIFARLYHRDGKHTYLKHMPLVMEYLLQAARRYTALTPLVRLIETLENKTP
                     QTGYTF"
     gene            1284..3524
                     /locus_tag="VFA14_01545"
     CDS             1284..3524
                     /locus_tag="VFA14_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HZS80107.1"
                     /translation="MMRFFKLSFRRFIDLWVSRFSTLVLAAAALPIPAQAGAVDDRDA
                     PIEVSAERMSGRLDHWLDLEREVEVVRGATTIKADRARYRVIQDEVEAAGHIWMKRLQ
                     DRYTGDEAMMNMDSGKGHVTSPTYLFGRHGGRGKAERIDFLSDDQALVTQGTYSTCEA
                     PDPDWYIQAATLDLDSGRDVGTMRNGVVYFKGVPILGAPLMSFSLSGERRSGVLPPTF
                     GVTSTGGVELTLPYYFNIAPNRDLTLYPRYITRRGLLLGAEGRYLGARYAGSTRVEGI
                     QDHMTGTGRYSLSTTHTQMLMPDLVLGWNVNKASDNDYSGDFVNSVTTGTQRLLARDI
                     SLTYTRTYWIAAGRVSRYQLLQDVNNPLQKPYDRLPQLSLTATRQDVGGFDLSFASEL
                     TRFSNTATTGASAGSEMVGGDRMYATLSVSWPLVRPSYFITPKATLDATTYRLNNVNA
                     GAPAHLTRVAPTYSLDAGMTFERDTRIAGGDVTQTLEPRLFYVRTPYRDQRQYPNFDS
                     ALADFNFAQIFSESRFVGHDRQSDADQITGALVSRFIEKNGLERLRMAFGQRFYFSDQ
                     KVTLDGATATVDGSKSDLLLFLGGQFTDKLALDNTVQYSQTLRQMARSTFGARWQPAP
                     KRVLNLTYRLDRTNPAGLLKQMDISGQWPVSQRWYAVSRTNYSIPDKTVAEGLLGMEY
                     KADCWVFRLVAQRVSTSSTRASTSFFIQLELNGLSRIGSNPLDALSNSIPGYQLINEP
                     DDITRY"
     gene            3536..4945
                     /locus_tag="VFA14_01550"
     CDS             3536..4945
                     /locus_tag="VFA14_01550"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HZS80108.1"
                     /translation="MNAFFSMRHHFSVRSTAVIVLSCLSFGMTDSAQAQLVLKKRPAA
                     PPSLKSAPTASKAPRPVDAIFVVVNDGVITYQELASRLQQLEKRLIAQGVALPPRAQL
                     QSQLLDRMIVERVQLQMARESGIVADDAMLDRAMSGIAAQNKLSVPDLRMRMERDGIS
                     FADFREDIRREIVMQRLREREVDNKVQVTESEVDNYLAAESHAAVRHEDLNLAQILIH
                     VPENAAPKQLAARRQRAEEALSQLKAGADFAQTAMAYSDSSDALRGGELGWRSQDRLP
                     QLFLDAVAALKPGEVSNVIKSGNGFHIIKLIDRRTTSDGKADAPTVEQTHVRHILIKL
                     NQVVSAAEARRKLLELKERLDHQAATFEELARLYSNDLSASKGGDLGWIYPGDTVPEF
                     ERAMNELKPGEISQPIESPFGFHLIQVLERKIDDVSQERVRQVVRRAIRERKIDEATE
                     DWLHQLRDRAYVEFRTDDD"
     gene            5008..>5212
                     /gene="pdxA"
                     /locus_tag="VFA14_01555"
     CDS             5008..>5212
                     /gene="pdxA"
                     /locus_tag="VFA14_01555"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078284.1"
                     /note="catalyzes oxidation of
                     4-(phosphohydroxy)-L-threonine into
                     2-amino-3-oxo-4-(phosphohydroxy)butyric acid which
                     decarboxylates to form
                     1-amino-3-(phosphohydroxy)propan-2-one
                     (3-amino-2-oxopropyl phosphate); Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase"
                     /protein_id="HZS80109.1"
                     /translation="MDRRPVLAITCGEPAGIGPEISIKAAWQLRADVRGVLLGDAAFL
                     TRMAQALDPAISLSPLSAQAFRNT"
BASE COUNT         1039 a         1443 c         1584 g         1146 t
ORIGIN      
        1 tgttgtcggc gctacccagg gtgctgggta cgagataaag aatgccaggc atgttgaaat
       61 cgttcctgca cgcgccatgt gcgacaacgt gctgttgaaa aagccacgga taggctgatt
      121 ttacaaggcc gtcgcatttc aaagaccttg aaagggatgg ttctcagaag gtatagccgg
      181 tttgcggcgt tttgttttcc agtgtttcaa tcaacctgac cagcggcgtc aacgcggtat
      241 agcgccgcgc tgcctgcagg aggtattcca tcaccagcgg catatgcttc aggtaggtat
      301 gcttgccgtc acgatggtaa agccttgcga aaatccccag tattttcaga tggcgctgta
      361 cgcccatgca ttcgaaatca cggtagaagg cgtcgatgtc cggcgccacc ggcaggccgg
      421 cgcgtctggc gcgttcccag tagcggatgg cccagtcgag caccatttct tcatcccacc
      481 ggatataggc gtcgcgcagc agtgacgcca gatcgtaggt gatcggtcca tagacggcat
      541 cctgaaaatc gaggatgccc ggattgctgt ccggcagcac catcaggttg cgcgagtggt
      601 agtcgcgatg gacatagacc cgggcttgcg tcaggttgtt gcccagcagt gtgtcgaata
      661 tgttggccag ggtgtttttc tggtcgtcgt tcaacgtgac ttcgaggtgt ctggtgagat
      721 accagtcagg gaacaattgc agctcgcgca gcagcagggc gcgaccatac tccggcagca
      781 ctccgggtct gctccgggtt tgcagcagca atagcgcatc gatggcgtcg aggtagagtt
      841 ggtgcgcgct gtccttgtcg agcagatgca gataagtggt cgaaccgagg tcggacagca
      901 gcaggaagcc gcgttcgaca tcctgcgcca gcacggtcgg caccgacatg ccggccgtgc
      961 cgaacacttc ggcgacatgg atgaagggcc gcacatcttc ctgcggtggc ggggcatcca
     1021 tgacgatgcg tgtttttcca tcgggggtat ctacgcggaa atagcggcga aagctggcgt
     1081 cggccgaggc aggacgtaag gtttcgatgt gggttggcgt ggtggcgaga gtggacagcc
     1141 atgtttgcag cagggacagg cgctggtcgg aatcaggtga aggcatgaac aatgggagga
     1201 gtgcgtttga ggatatcgag ttcccctata atacgggatt attttcagaa aatcgccagc
     1261 cctatgttta cacgctgctt tatatgatgc gctttttcaa gctgtctttt aggcgtttca
     1321 ttgacttgtg ggtgtcgcgt ttttctacgt tggtgctggc tgcggctgcc ttgcccattc
     1381 cggcgcaggc cggggcggtc gatgacaggg atgcgccgat cgaggtcagt gcggaacgga
     1441 tgagcggccg tctggatcac tggctcgatc tggaacgtga ggtcgaagtc gtgcgtggtg
     1501 cgaccaccat caaagccgac agggccagat accgcgtcat ccaggatgaa gtcgaagctg
     1561 ccggtcatat ctggatgaag cgtttgcagg atcgttatac cggtgatgag gccatgatga
     1621 acatggattc gggcaagggc catgtcacca gcccgaccta tctgttcggt cgtcacggcg
     1681 gacgcggcaa ggcggaaaga atcgactttc tgtccgatga tcaggccctg gtgacgcaag
     1741 gtacttatag cacctgtgag gcgcccgatc cggattggta catacaggca gccacgctcg
     1801 acctcgacag cggccgcgat gtcggcacca tgcgcaatgg cgtggtgtat ttcaaaggcg
     1861 tgccgattct gggggcgccg ctgatgtctt tctccttgtc gggcgagcgc aggtccggcg
     1921 tgctgccgcc gaccttcggc gtgaccagca ccggcggcgt cgaactgacg ctgccctatt
     1981 atttcaatat cgctcccaat cgggatctga cgctgtatcc gcgttatatc acgcggcgtg
     2041 gtctgctgct gggggcggaa ggccgctatc tgggcgcaag gtatgcgggc agtacacgtg
     2101 tcgaaggtat acaggatcat atgaccggta ctggccgtta ttcgctgtcc acgacgcata
     2161 cgcagatgct gatgccggat ctggtgctcg ggtggaatgt caacaaagct tccgataacg
     2221 attattccgg cgattttgtg aattcagtga caaccggcac gcaacgtttg cttgcgcgtg
     2281 atatcagtct gacctatacc agaacctact ggattgcagc tggtcgcgtg tccaggtacc
     2341 agttgctgca ggacgtgaac aatccgctcc agaagcccta tgaccgcttg ccgcagctga
     2401 gcctgacggc gacgcgccag gatgtcggcg gcttcgatct gagtttcgcc tctgaactga
     2461 cgcgcttttc gaataccgct accacgggtg cctcggccgg ttcggaaatg gttggcggcg
     2521 accgcatgta tgcgacgctg tcggtttcct ggccgctcgt tcgtccaagc tacttcatta
     2581 cacccaaggc gacgctggat gcaacgacct accggctcaa caacgtcaac gccggcgcgc
     2641 cggcgcatct gacgcgcgtg gcgccgactt actcgctgga tgcgggcatg actttcgaac
     2701 gtgatacgcg tattgccggt ggtgacgtga cccagacgct ggaaccgcgc ctgttctatg
     2761 tgcgcacgcc gtatcgtgat caacgtcagt atccgaactt cgattctgct ctggcggact
     2821 tcaacttcgc acagattttc agcgagagcc gctttgtcgg tcatgaccga caaagcgatg
     2881 ccgaccagat caccggtgcg ctggtgtctc gcttcatcga aaaaaatggt ctcgagcgcc
     2941 tgcgtatggc atttggccag cgcttctatt tctctgacca gaaggtgaca ctggacggcg
     3001 cgaccgccac ggtcgacggc agcaagtccg acctgttact gttcctgggc ggtcagttta
     3061 ctgataagct ggcgctcgac aataccgtgc agtacagtca aaccctgcgc cagatggcgc
     3121 gctcgacttt cggcgcgcgc tggcagccgg cgccgaagag ggtattgaac ctgacgtatc
     3181 gccttgatcg caccaatcct gccggtttgc tcaagcagat ggatatatcc ggtcagtggc
     3241 cggtatcgca acgctggtat gctgtcagcc gtaccaacta ttctattccc gacaagaccg
     3301 ttgcagaagg tctgctgggg atggaatata aggccgattg ctgggtgttc cgtctggttg
     3361 cccagcgtgt ctcgacgtcg tcgaccaggg cgtcgacctc gttctttatc caactggagt
     3421 tgaacggcct ttccaggatc ggttccaatc cgctggatgc gttgagcaac agcatcccgg
     3481 gctatcagct catcaatgag cctgatgaca ttacacgtta ttaaccgaca tgtttatgaa
     3541 cgcttttttt tccatgcgcc atcatttttc tgtacgcagt acggccgtca tcgtcctgtc
     3601 gtgcctgtcg tttggcatga ctgacagcgc ccaggcgcaa ttggtcctca agaagagacc
     3661 cgctgcgccg ccgtcgctca agtcggcgcc gactgcgtcg aaggcgccgc gtccggtcga
     3721 tgcgattttt gtggtcgtca atgatggcgt gattacctat caggaactgg ccagtcgtct
     3781 gcagcaactg gaaaaacggc tgattgccca gggcgtggcg ttgccgccgc gcgcgcagct
     3841 acagagccag ttgctcgatc gcatgattgt cgagcgtgtg cagctgcaga tggccaggga
     3901 aagcggcatc gtcgccgatg atgccatgct cgatcgcgcc atgtccggca ttgccgcgca
     3961 gaacaaacta tcggtgccgg atttacgtat gcggatggaa cgcgatggca tctcctttgc
     4021 cgatttccgt gaagacattc gcagagagat cgtcatgcag cgtctgcgtg agcgtgaagt
     4081 cgataacaag gtgcaggtca ctgagtcgga agtcgataat tacctcgccg cggaaagtca
     4141 tgccgcagtc aggcatgaag atctcaatct ggcgcagatt ctgatccacg taccggaaaa
     4201 tgccgctccg aaacaacttg ccgcacgccg tcagcgtgcc gaggaggcgc tgagtcagct
     4261 caaggccggc gccgatttcg cccaaaccgc gatggcctac tccgacagca gtgacgcctt
     4321 gcgcggcggt gagctcggct ggcgttccca ggaccgtttg ccgcaattgt ttctggatgc
     4381 ggttgctgct ctgaaaccgg gcgaagtctc caacgtcatc aagagcggca atggctttca
     4441 tatcatcaag ctgatcgatc gccgcactac cagcgatggc aaggccgatg cacctaccgt
     4501 cgaacaaacg catgtgcgtc atatcctgat caagctcaat caagtggtgt cggctgccga
     4561 ggcgcgccgg aaactgctgg aactgaaaga gcgtctggat caccaggctg ccacgttcga
     4621 ggagctggcc agactgtatt ccaatgactt gtctgcttcc aagggcggcg atctgggctg
     4681 gatttatccc ggcgatacgg tgcctgaatt cgagcgtgcc atgaatgaac tcaagccggg
     4741 tgaaatcagc cagccgatcg aatcgccctt tggtttccat ctgattcagg tactcgaacg
     4801 caagatcgac gatgtttcgc aggaacgggt gcgccaggtt gtgcgtcggg caatccgcga
     4861 acgcaagatc gacgaagcga ctgaagactg gctgcaccag ttgcgtgatc gcgcctatgt
     4921 cgagttccgt accgacgacg actgatttgc ctgatgtccg atctctcaga acgcgctcat
     4981 gatcccgccg ggagtcgcac tatcgtcatg gaccgacgtc cggtgctggc catcacctgc
     5041 ggagaaccgg ccggtatcgg tccggaaatt tccatcaagg cggcatggca actgcgcgcc
     5101 gatgtgcgcg gcgtgctgct cggtgatgct gctttcctga cacggatggc gcaggcgctg
     5161 gatccggcga tctccctgtc gcccttgtcc gcacaggctt tcagaaacac cg
//
LOCUS       DASRDH010000063         3406 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1590112,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000063 DASRDH010000000
VERSION     DASRDH010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 3406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3406
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1590112"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1324)
                     /locus_tag="VFA14_01560"
     CDS             complement(<1..1324)
                     /locus_tag="VFA14_01560"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal sensor histidine kinase"
                     /protein_id="HZS80110.1"
                     /translation="MTFAGVGTYLYHSLSRQLETRDDEELLGKIRSMSHLVQEAPSVE
                     SIRRDPHIFLDAAAGHDQLTVLIRVGNEALVVNSNPGIQALSFLIPPVNSVDLNRSAV
                     SVIKLRNGESARAVATETIINKTNERVQIMVARTASDRMQLLSNYQAEVWMAALCGTS
                     LATVLSYFLVLRGLRPLRVVASQAQSITAHRLDTRLDMEAAPQELHEIVQSFNAMLDR
                     LHGSFERLTQFSSDLAHDLRTPLNNLMVQTQVAISQARTLEEYQSLLISNIEEYERLA
                     RMTESILFLARAEHAHVAVNKESLDMAVELTRIAEYFEGIADDAGVIINVSGQGKLYA
                     DTILLRRAVSNLVANAIRYTPFGHTIKLSAESQHDSVTISVTNPGKGIDASHLDRIFD
                     RFYRVDTARSSSATSTGLGLAIVQSIMALHGGTAKAESVIDGVAVFRLI"
     gene            complement(1387..1503)
                     /locus_tag="VFA14_01565"
                     /pseudo
     CDS             complement(1387..1503)
                     /locus_tag="VFA14_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007734506.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
     gene            1453..>3406
                     /locus_tag="VFA14_01570"
     CDS             1453..>3406
                     /locus_tag="VFA14_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017449872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HZS80111.1"
                     /translation="MVQFRTQAPNGNINNVGTDIYFARQLVNERIQEARSKLPPGIDP
                     GMGPISTGLGEIYLWTVETQDGAKKPDGSEYTPADLREIQDWIIKPQLRNVPGVTEIN
                     SIGGYAKEYHVAPSPEKLASYGLTLQSVATALEKNNGNVGAGYIERHGEQYLIRAPGQ
                     VNSIDDIGNIILGNVQGVPIRIKDVAEVGIGRELRTGAATENGREAVLGTVFMLIGEN
                     SRTVSQAVDKKMVEINRSLPEGVKAVTVYDRTILVDKAINTVKKNLVEGAILVIAVLF
                     VFLGNIRAALITATVIPLAMLFTFTGMIANHVSANLLSLGALDFGIIIDGAVVIVENC
                     VRRLAHAQEHHGRPLTQAERFHEVFAASCEARRPLLFGQLIIMIVYLPMFVLTSVEGK
                     MFQPMAFTVVAALLGAMVLSVTFIPAAIALFIGEKVAEKENGLMRGAKRLYKPLLGKV
                     MLNKPVVLTFATALIVLCAVIGSRMGSEFVPHLNEGDLAIQALRIPGASLTQSIEMQQ
                     QIERSLKQKFPEIERVFARTGTAEVASDPMPPNISDGYIMLKPLDQWPEPRKTQAELL
                     EAIQETVAKIPGNSYEFSQPIQLRFNELISGVRSDVAVKIFGDDMTILNDSAAKISSV
                     LEKIQGATEVKVEQTTGLPMLTVQINR"
BASE COUNT          821 a          876 c          928 g          781 t
ORIGIN      
        1 aaataagtct aaagacagct acaccatcaa tgacgctctc ggccttcgca gtgccaccat
       61 gtaaagccat aatagattgc acgatcgcca atccgagtcc tgtcgacgtt gccgagctgc
      121 tcctcgcggt atcaacacga taaaagcgat caaaaatacg atcaagatgg ctggcatcga
      181 tgcccttccc agggttcgtt acactgatag tcacagaatc gtgctggctt tcagccgaca
      241 atttgatggt atggccgaat ggtgtataac gaatagcatt ggcaacaagg ttgcttaccg
      301 cacgtcgcag taagattgtg tcggcgtaaa gtttgccttg gcctgaaaca ttgatgatca
      361 caccggcgtc gtctgcaatc ccttcaaaat attcggcgat acgtgtcagc tccactgcca
      421 tgtcgagaga ctctttgttg acagcgacgt gggcatgttc agcccttgca aggaagagaa
      481 tactttctgt cattctcgcc aggcgttcat attcttcgat gttagagatg agcagcgact
      541 gatattcctc aagagtacgt gcctgcgaaa tggcgacctg tgtctgaacc atgagattgt
      601 tgagtggtgt gcgcaggtca tgggcaaggt cggacgagaa ctgcgtcagc cgctcgaaac
      661 tgccatgtaa gcgatccagc atggcattga atgactgcac tatttcgtgt aactcctgag
      721 gcgcagcctc catatccagt cgggtatcca aacgatgcgc tgtgatgctt tgcgcttggc
      781 tggcaaccac gcgtaacggg cgcaggccgc gcaagaccag gaaataactt agtacggttg
      841 ccagcgacgt tccgcacaag gcagccatcc atacctctgc ctggtaattg ctcaacagct
      901 gcattctgtc agaggcagta cgagccacca tgatctgcac ccgctcgttc gtcttattga
      961 tgatcgtctc tgtggcgacg gcacgcgcgg attcgccatt gcgaagcttt ataacggata
     1021 ccgcagaccg attgagatct acgctgttta ctggcggtat aaggaaagac aaggcttgga
     1081 tcccaggatt gctgttgaca accaaagcct cgttgccaac ccgtatgagg accgtcagtt
     1141 ggtcgtgacc ggcggcagca tctaggaaga tatgcggatc acggcggatc gactcaacgg
     1201 aaggagcttc ttgaaccaga tggctcatcg agcgtatttt tccaagaagc tcttcgtcat
     1261 cccgggtttc cagttgtctg gagagcgagt gatagaggta ggtgccaaca cccgcaaagg
     1321 tcacgatcgc gactagtccg aacagagaga ccaggcgtga agtcagtgac gctggttgta
     1381 gtcgattcac gaacgcacct ctaacacgta acccatcccg cgcaccgtgt ggatgagctt
     1441 ctgtgtgtgt ggatcgtcca gtttcgcacg caggcgccga acggcaacat caacaacgtt
     1501 ggtacggaca tctactttgc gcgccagcta gtcaatgaac gcatccagga agcacgtagc
     1561 aagctgccgc cgggaatcga tccggggatg gggccgattt ctactggtct tggggaaatc
     1621 tacctctgga cggtcgaaac ccaagacggc gccaaaaagc cggacggttc tgaatatacg
     1681 ccggccgatt tacgcgagat ccaggattgg atcatcaagc cgcagttgcg caacgtgcca
     1741 ggtgtcaccg agatcaattc gatcggtggt tatgccaagg aataccacgt ggcgccatcg
     1801 cccgagaagc tggcctccta tgggttgaca ctgcaatcag tggcgaccgc cctggaaaaa
     1861 aataatggta acgtcggcgc cggctatatc gagcgtcatg gcgaacaata tctgattcgc
     1921 gcacccggcc aggtcaattc gatcgacgat atcggcaaca ttatcctggg caacgttcag
     1981 ggtgttccta ttcgtatcaa ggatgtcgcc gaagttggca tcggccgcga attgcgtacc
     2041 ggcgctgcta ccgagaatgg tcgtgaggct gttttgggta cggtcttcat gctgatcggt
     2101 gaaaatagtc gtaccgtctc acaggccgtc gacaagaaga tggtcgaaat caaccgttcg
     2161 ttacccgaag gcgtgaaagc agtcacggtc tatgaccgta caattctggt cgacaaagcc
     2221 atcaatacgg tgaagaaaaa cttggtcgag ggcgcaattc tggtgattgc ggtgctgttc
     2281 gtcttcctcg ggaatattcg tgcagccttg attacggcaa cggtcatccc attggccatg
     2341 ctgttcacct tcaccggcat gatagcgaat catgtgagtg ccaatttgct aagtttaggc
     2401 gcactcgact ttggcatcat tattgatggc gccgtcgtga ttgtagagaa ctgcgtgcgt
     2461 cgactggcgc atgcgcaaga acatcacggc cggccactga cgcaggcaga acgcttccac
     2521 gaagtatttg ccgcttcctg tgaagcacgc cgccctctcc tgttcggtca attgatcatc
     2581 atgatcgtct acctgccaat gttcgtgttg acgagcgtcg aaggcaagat gttccagccg
     2641 atggcattta ccgtggttgc cgcactgctg ggcgcgatgg ttttgtcggt gacgttcatt
     2701 ccagcagcaa ttgcactctt catcggtgag aaggttgccg agaaagaaaa cggcttgatg
     2761 cgtggtgcaa aacgtctgta caagcccttg ttgggtaagg taatgctcaa taagcctgtc
     2821 gtgttgacgt tcgctacagc actgattgta ctgtgcgccg tgattggctc ccgcatggga
     2881 agtgagtttg ttccgcattt gaacgaaggt gatctggcga tacaggcatt gcgtatcccg
     2941 ggcgccagct tgacgcaatc gattgagatg caacaacaaa tcgaacgttc attgaaacaa
     3001 aaatttcccg agatcgagcg tgtttttgcc cggactggta ccgccgaggt agcttctgac
     3061 ccgatgccac caaacatttc ggacggctac atcatgctca agcctttgga tcaatggccg
     3121 gaaccaagaa agacacaagc cgagctgctc gaagcaattc aggaaacagt cgcgaagatt
     3181 ccaggcaaca gctatgaatt ctcgcaaccg attcaattgc gctttaacga gctgatctcc
     3241 ggcgtgcgta gcgatgtggc ggttaaaatc ttcggtgacg acatgaccat cttgaatgat
     3301 agcgcggcca aaatctcgtc ggttctggag aaaatccaag gtgcgacgga agtgaaggtt
     3361 gagcaaacaa ccggcttgcc gatgctcacc gtccagatca accgtg
//
LOCUS       DASRDH010000064         2857 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1110450,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000064 DASRDH010000000
VERSION     DASRDH010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1110450"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="VFA14_01575"
     CDS             complement(<1..447)
                     /locus_tag="VFA14_01575"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HZS80112.1"
                     /translation="MFVVRPVETRDISALVSLGASVRPGVHTLPRTLPAVEHAVQRSL
                     VSFAARVDIPSEEAYFFVLESTDDGSVIGAAAITATAGAGGTFFAFRNDVIQQVSRDL
                     DISHSVHVLTLCSDLSAYSQLSGFYAHDRCQAGRQAELLSRARLLFA"
     gene            complement(464..1714)
                     /locus_tag="VFA14_01580"
     CDS             complement(464..1714)
                     /locus_tag="VFA14_01580"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017879610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine/succinyldiaminopimelate
                     transaminase"
                     /protein_id="HZS80113.1"
                     /translation="MQQRDVHAVMEEQPVVRSTFDEVMVPVYAPSTLVPVRARGLELW
                     GQDGKRYLDFTAGIGVTGLGHAHPVVVEALRQQAETLWHVGNGYTNEPVLRLARALTQ
                     ATFAERIFFSNSGAEANEAAFKLARKYAHEKSGPRKSRIISCTNAFHGRTLFTVSVGG
                     QPKYTEGFAPLPADISHIPFNDISAAQAAIDDDVCVVVVEPIQGEGGVLPATPEYLQA
                     LRDLCDASGALLIFDEVQCGMGRTGSLFAYTRYAVTPDILTAAKALGNGYPVGAMLTT
                     SVIASAFSVGSHGTTYGGNPLAAAVALSVLKTIDTPAFLARVRQASDQLLQALEAIRQ
                     DYPQVFSEVRGSGLMLGMVLTDAWQGRAKEIARCAEAQGLMVLIAGTSVLRLLPALIV
                     SDAQIDEADLLLRRALDAFLLAQQ"
     gene            complement(1863..2375)
                     /locus_tag="VFA14_01585"
     CDS             complement(1863..2375)
                     /locus_tag="VFA14_01585"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013433933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZS80114.1"
                     /translation="MSRAPFQLALLDTAYDRAGFDSDSESLNRYLREQVTQDIRRRVA
                     ACFVALADEQRIAGYYTLASASLLLADLPASTGKKLPRYPTVPAVRKGRLAIDQAFKG
                     QGLGGALLADALDRAARSEIAAFALVVDANDETAADFYRHHGLIALPDSPRTLFLPLA
                     TVLPSRNSPR"
     gene            complement(2372..2560)
                     /locus_tag="VFA14_01590"
                     /pseudo
     CDS             complement(2372..2560)
                     /locus_tag="VFA14_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236962.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
BASE COUNT          673 a          880 c          823 g          481 t
ORIGIN      
        1 cgcaaaaagc aggcgcgcgc gcgacagcag ttcggcctgc cgcccggcct ggcaacggtc
       61 atgcgcataa aaccctgaca attgcgaata agcgctcagg tccgagcaca aggtcagcac
      121 atgcacgcta tgactgatgt cgaggtcgcg cgaaacctgc tggatcacgt cattgcgaaa
      181 ggcgaagaaa gtcccgccgg ccccggccgt ggcggtaatc gcagcagcgc cgatgaccga
      241 tccatcatcg gtcgactcca gcacaaaaaa atatgcctct tcactgggaa tatcgacacg
      301 ggcggcaaac gaaaccagcg aacgctgcac cgcatgctcg accgccggta gcgtcctggg
      361 caaggtatgc acgcccgggc gcacgctcgc ccccaatgac accagcgcgg aaatatccct
      421 ggtttctact ggccggacga caaacatgga tcctccagcc tgcttactgc tgcgccagca
      481 gaaacgcatc cagcgcacgc cgcaacaaca ggtccgcctc atcgatttgc gcatcggaca
      541 cgatcagcgc gggcagcaga cgcagcacac tcgtcccggc gataagcacc atcaagcctt
      601 gcgcttcggc gcatctggcg atttccttgg cccgcccctg ccaggcatcc gtcagcacca
      661 tgcccagcat cagaccactg ccgcgcacct ccgaaaaaac ctggggataa tcctgccgga
      721 tcgcttccag cgcctgcaac aattgatccg atgcctgccg gacacgcgcc agaaaagccg
      781 gcgtatcgat cgttttcagc acgctgagcg ctaccgcggc cgccagcgga ttaccaccgt
      841 aagtcgtgcc gtgggaacca accgagaagg cgctggcgat gacgctggtg gtcagcatcg
      901 cgccgaccgg atagccgttg ccgagcgcct tggcggcagt caggatatcc ggcgtcactg
      961 catagcgggt gtaagcgaac agggagccgg tacgccccat gccgcactgc acttcatcaa
     1021 aaatcagcaa ggcgccgctg gcatcgcaca agtcgcgcaa ggcttgcaga tactcaggcg
     1081 tagccggcaa gacgccaccc tcgccctgta tcggctcgac cacgacgacg cagacgtcat
     1141 cgtcgatggc ggcctgcgca gcactgatgt cattgaatgg aatgtggctg atatcagccg
     1201 gcagcggcgc aaagccttcc gtatatttag gctgaccgcc gaccgatacg gtaaacaagg
     1261 tgcgcccgtg gaaagcgttg gtgcaggaaa tgatgcggga tttgcgcgga ccggattttt
     1321 catgcgcata tttgcgcgcc agcttgaagg cggcttcatt cgcttccgcg ccggaattgc
     1381 tgaagaatat acgctcggca aacgtagcct gcgtcaatgc gcgcgccagc cgcaataccg
     1441 gttcgttggt ataaccattg ccgacatgcc ataaggtttc cgcctgctgg cgcagggcct
     1501 cgaccacgac aggatgggca tgcccaagac cggtgacgcc aatgccggca gtgaaatcga
     1561 gataacgctt gccgtcctgc ccccacaact ccagtccgcg cgcgcgcacc ggaaccaatg
     1621 tgctgggcgc atagaccggc accatgacct cgtcgaaagt gcttctgacg accggctgct
     1681 cttccatgac tgcatggaca tcgcgttgct gcatcacatt catacctttc cccccatcga
     1741 attcatcctg gatgggttga ttataaaaaa gccggcgggt aaattctttc gcaacagcga
     1801 cgccggattt cataaaaaag acaggtgata taaaaaggcc aaggcttctc gccacaaagg
     1861 agttaacgcg gcgagtttcg agaaggcaga acggttgcca gaggcaaaaa caaggtgcgt
     1921 ggcgagtctg gcaaagcaat caggccatga tgccggtaga agtccgctgc cgtctcgtca
     1981 ttggcatcca ccaccagtgc gaaagcagca atttcagagc gggccgcccg gtcgagcgca
     2041 tcggccagca gcgcaccacc taaaccctgg cccttgaaag cctgatcaat ggccaagcgt
     2101 cccttgcgga cggctggcac ggtcggatag cgcggcaact tcttgccggt gctggccgga
     2161 agatcggcca gtagcaggct cgccgaggcc aaggtgtaat agcccgcgat acgctgctca
     2221 tcagccaagg ccacgaaaca ggccgccacg cgccggcgaa tgtcctgagt gacctgctcg
     2281 cgcaggtaac ggttcagaga ctccgaatcg ctatcgaacc cggcgcggtc atacgcggta
     2341 tcgagcagcg cgagctggaa cggcgcacgg ctcattcagt gcgcaagagc ctactgcgac
     2401 gggcgatagc ccgttccaaa gccggtgact gctgcggtgg cgacagcaac gcctgggcga
     2461 aacactcctg atcagccaga gacaggcgga taacttcggc ctgctcgatg gcgcgctgcg
     2521 cggcatcccg gacggcggca accacgaaat cggtcatggt aggcgaaatt ttcggcgttt
     2581 ccggcttaaa tagacaggag caggctagta ttaatgttga tgcgcaacgt ttcccagctg
     2641 tagtcaaagc tgcacatttc gcaaacgaaa tgctaaagta accagctgga taatgagggg
     2701 tactgtaatg gcaacaaaaa aacccactga aaagaaaaat gtgccgcgac agggccgctt
     2761 gcaagcaccg aaacgcggtc gtgttaccaa tggcgttacc ggtccaaaac cgcagccgat
     2821 caagcccggt agtggatcgc aaggtggcag caacggt
//
LOCUS       DASRDH010000065         8168 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_308739,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000065 DASRDH010000000
VERSION     DASRDH010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 8168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8168
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308739"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="VFA14_01595"
     CDS             <1..369
                     /locus_tag="VFA14_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006462431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZS80115.1"
                     /translation="TQTTPYAVMVMVLLAGGCFRSLQFTSLNAISYADVDTRKLSQAT
                     SIASVAQQLSVGMGVVLGGFALEMSSHVQGHTTLMAQDFWPAFLTVAAASACSIPLLM
                     RLPANAGAALAGRNTAAKST"
     gene            406..1386
                     /gene="corA"
                     /locus_tag="VFA14_01600"
     CDS             406..1386
                     /gene="corA"
                     /locus_tag="VFA14_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008327273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HZS80116.1"
                     /translation="MIDANMLVNCTVYKNGRKLRDVSIGEINDALEEDNTFVWVGLFE
                     PSVELMRQIKEEFRLHELAVEDAYAAHQRPKIEEYGDTLFVVLQTAQAVEGAVQFGET
                     HIFVGARFVVSVRHGASHSYARVRERCESLPERLARGPGYVLYAIMDFVVDHYRPVMD
                     DLEARFDRFEEELFGRDSERAHLQDLYRLKRQLIKIRSAVFPLLDVCSQLLRFHESLI
                     TKDGKVYFRDICDHVAHLVETGDRLRDMVAAAMQIDLAQISIRQNEVVKSLAGWGGIL
                     AIPTMVFSLYGMNFRYMPELEWYWSYPAVLVSIAASCAWLYFRLRRIGWL"
     gene            complement(1452..1528)
                     /locus_tag="VFA14_01605"
     tRNA            complement(1452..1528)
                     /locus_tag="VFA14_01605"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1492..1494),aa:Arg,seq:tct)
     gene            complement(1609..1685)
                     /locus_tag="VFA14_01610"
     tRNA            complement(1609..1685)
                     /locus_tag="VFA14_01610"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1649..1651),aa:Pro,seq:tgg)
     gene            1953..3627
                     /locus_tag="VFA14_01615"
                     /pseudo
     CDS             1953..3627
                     /locus_tag="VFA14_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007879166.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
     gene            3751..4926
                     /locus_tag="VFA14_01620"
     CDS             3751..4926
                     /locus_tag="VFA14_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005674384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZS80117.1"
                     /translation="MDPVRNPFAPGAGSPPPELAGRQAIIEDARIAIDRALLGKPSRS
                     QIFLGLRGVGKTVLLNAVEMMANERGHLTSSIEAPENKSLGELLLPRVHQVLRKLSLA
                     GNAKAKAHDALRALRSFASAFKFEYGDMSIAVDPEVGVADSGDLENDLPELFVRIGET
                     AKAGGKAWTLLIDEVQYLSQKDLAALIVSLHKINQSKLPVLFFGAGLPQVAALSGEAK
                     SYAERLFHYPEVGALSEADAKEAIQQPVSEEGAVIDDDALTEIFHKTKGYPYFLQEWG
                     HQSWNLAVGNTITVQDAKHAETTTLKRLDEGFFKVRFDRLTPKEREYVIAMAKLGDGP
                     YRSSDVADALNESVQSLGPRRAQTISKGMIYSPSHGDIAFTVPMFNDYLMRNLTNLG"
     gene            complement(4976..6169)
                     /locus_tag="VFA14_01625"
                     /pseudo
     CDS             complement(4976..6169)
                     /locus_tag="VFA14_01625"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(6308..6886)
                     /locus_tag="VFA14_01630"
     CDS             complement(6308..6886)
                     /locus_tag="VFA14_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS80118.1"
                     /translation="MQAELLSLSRSFHYYRSRPIKGNAYVCVYNVGHENLDYHRHTHI
                     SDITYCAAGRLLLELPQLSKSYVFYPGQVVQIPYDTIHRVSHYSDSESHSRYILVQIG
                     KFSIDFIRDDGIVVDGNPSNLRNKGLDCYIGDQGDRLRDIAIAFRECQPDDLSDAEYT
                     DVIAALDAICHNGIARSHTNDTLFHQLTTLGY"
     gene            complement(7164..>8168)
                     /locus_tag="VFA14_01635"
     CDS             complement(7164..>8168)
                     /locus_tag="VFA14_01635"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007877813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HZS80119.1"
                     /translation="KAEISARIASGAAQLIIGTHALIQDKVQFAKPGLVIVDEQHRFG
                     VGQRLALRNKSFGAGNSVPHQLMMSATPIPRTLAMTYYADLEVSVIDELPPGRTPVVT
                     RTIDQNRRAEVIARVHAAAQEGHQIYWVCPLIKESETLQLQTATATHAMLAEALPDLH
                     IGLVHGRLKPAEKQDVMDAFNRGGMHVLVATTVIEVGVDVPNASLMVIEHAERFGLAQ
                     LHQLRGRVGRGRAASVCLLLYQSPLGGAARQRLMTMRETTDGFEIARRDLEIRGPGEF
                     LGARQSGQALLRFADLATDQWLVEKARDLAQQLLHSDDAIVDAHLQRWLGGREDFLKV
                     "
BASE COUNT         1849 a         2124 c         2360 g         1835 t
ORIGIN      
        1 acccagacca cgccgtatgc ggtgatggtg atggtgctgc tggccggcgg ttgtttccgt
       61 tcgctacagt tcaccagcct caatgcgatt tcctatgccg atgtcgacac gcgcaagttg
      121 agccaggcaa ccagtattgc cagcgtggcg cagcagctat cggtcggcat gggagtggta
      181 ctgggcggct tcgcgctgga gatgtccagt catgtgcaag gccatacgac gttgatggcg
      241 caggatttct ggccggcctt tctgacggtc gcagcagcgt ctgcctgctc gattcccctg
      301 ctcatgcgcc tgccagccaa tgccggcgcc gcactggctg gacgcaatac cgccgccaaa
      361 tcgacatgat cgcgcgctgc tgatcaacag gaacaggagg aaagcatgat cgacgcaaat
      421 atgctcgtca actgcacagt ctataagaac ggccgcaagc tgcgtgatgt cagcatcgga
      481 gaaatcaacg atgcgctcga ggaagacaac accttcgtct gggtcggcct gttcgaaccc
      541 agcgtcgagc tgatgcggca gatcaaggaa gaattcaggc tgcatgaact ggcggtcgag
      601 gatgcctatg ccgcgcatca gcggcccaag atcgaagagt acggcgacac cctgttcgtg
      661 gtgctgcaaa cggcccaggc tgtcgagggg gccgtgcaat tcggtgaaac ccatatattc
      721 gtcggcgccc gtttcgtggt gtcggtgcgg catggcgcat cgcacagcta tgccagggtg
      781 cgtgaacgct gcgaaagcct gccggaacgc ctggcgcgcg gtcccggcta cgtgctgtat
      841 gcgatcatgg atttcgtggt tgatcattac cgtcctgtca tggacgatct tgaagcgcgt
      901 ttcgacaggt tcgaggagga gttgttcggg cgcgattccg agcgcgcaca tttgcaggat
      961 ttgtatcggc tgaaacgcca gctgatcaag atccgctcgg cggtctttcc cttactggat
     1021 gtttgctcgc agctgctgcg ttttcatgaa agcctcatca ccaaagacgg caaggtttat
     1081 ttccgtgaca tttgcgacca tgtagcgcac ctggtcgaga ctggtgaccg cctgcgcgat
     1141 atggtggcgg cggcgatgca gatcgatctg gcgcagatca gcattcgcca gaatgaagtg
     1201 gtgaaatccc tggcaggctg gggcggcatt ctggcgattc cgacgatggt attcagcctg
     1261 tacgggatga acttcaggta catgcctgag ctggagtggt actggagtta tccggcggtc
     1321 ctggtgtcga tcgccgcttc ctgcgcctgg ctgtattttc gcttgaggcg gataggctgg
     1381 ctgtaggaga gtctgctgaa ccccgaatgg gcgtgtgact ggaatcagcc ggaagaacgg
     1441 aagggaagaa gtggcctgcc ctaagggaat cgaaccccta acctatggct tagaaggcca
     1501 ttgctctatc cggttgagct aagggcagat gagccaggca tgaaatgccg tgtccgatat
     1561 cgtcttcagc aacgaaaacg ggctgtcttg cgacagcccg ttcatgtgtg gtcggagtac
     1621 aaggattcga accttggacc ccctggtccc aaaccaggtg cgctaccggg ctgcgctaca
     1681 ctccggaaaa cgagatgata tccagacatg ggcatgcggt caatgttttc ttcgaggttt
     1741 cttctttcgc atccttgccc cgaacacgga atcggacttg gaatacgcta taatcccggc
     1801 tcccaggatg tctcctggca tcattcatgc cggggaacag agggacgaag ggttggattt
     1861 tccagccctt tttcgtttcc atcacatccc ttctcgaccg aacgggacct atgaaatagt
     1921 gcctagcacg ctctacgact cacagatgca cgatgcaaga ccttacccct gattccgcga
     1981 atgaagatgc cggctcacgc ggcaaagtcc cgcccggatc gattgcgcgt gaagcggcgc
     2041 ggcgccgcac gttcggcatc atctctcacc cggatgcagg gaaaaccacg ctgaccgaaa
     2101 agctgctgct gttctcgggt gcgatccagc tggcgggcac tgtcaaggcg cgcaagagca
     2161 gtcgccatgc gacctccgac tggatggaaa tcgaaaagca gcgcggtatc tcggtggcca
     2221 gttcggtcat gcagttcgag tatcgcgatc acgtcgtcaa tctgctcgat acgccaggcc
     2281 accaggattt ttcggaagat acctaccgcg tgctgacggc agtcgactcg gcgctgatgg
     2341 tgatcgacgc cgtcaagggc gtggaagagc agaccatcaa actgctcgat gtctgccgca
     2401 tgcgcgacac gccgatcatc accttcatga acaagatgga ccgcgaaacc cgcgatccac
     2461 tggaattgct cgacgagctg gaatcagtcc tgaagattca atgtgcgccg gtgacctggc
     2521 cgatcggcat cggcaggaat ttccgcggcg tgtatcactt gctgcgcgat gaaatcatgc
     2581 tgttcaaggc gggtgaagag cgcgccgacg gcagcgttga cctcatccag ggcatagcca
     2641 gtcccagact ggccgagatg tttccgctgg aaatcgaaca gctcaggatg gaggtcgaac
     2701 tggtgcatgg cgcttcgcac cgattcagtc tggacgattt tctggcgggt atccagacgc
     2761 cggtattttt tggttccgcg atcaacaact tcggcgtgcg cgagattctc gacgcgctgc
     2821 tcgactgggc tcccggtccg ggcgcgcgtg acgctaccat gcgcagcgtg gcgccgactg
     2881 aggcgccgtt ctcgggtttc gtgttcaaga tacaggccaa tatggacccg gcgcaccgcg
     2941 atcgcatcgc tttcctgcgt gtgtgttcgg gccgcttcga gcgcggcatg aagctcaaac
     3001 atctgcgttt gaaccgtgac atcaaggcat gcagtagtaa gcttcatggc atcgagccgc
     3061 gggcaggtgg aagaggctta cgccggtgac atcattggtt tgcccaacca cggcaacatg
     3121 cagatcggcg acagcttctc cgaaggcgaa ctgctgcaat tcaccggcat cccttatttt
     3181 gcgccggatt tctttcgcgt ggtgcgcatc cgtaatccgc tcaagatcaa gcaattgcac
     3241 aagggcttgc agcagctcgg tgaagaaggc gcggtgcagg ttttcaggcc ggccaacaat
     3301 agcgacctgg tgctgggcgc ggtcggcgta ttgcagtttg aggtcgtcgc gagccgtctg
     3361 ctcaatgaat acggggtcga tgccgtgttc gaaggcagca atatcagcag cgcgcgctgg
     3421 gtcagctgtg acgaccggaa gatgctggcc gatttcgaaa aatcttccgc tggccaccat
     3481 ctggcgcgcg atgccgccgg caatctggcc tatctggcga cctcgggcgt gaacctgcgc
     3541 ctggcgcagg aacgctggcc ggaagtcact ttccatacga cgcgcgaaca cgcggccaga
     3601 ctggatgaat cagggttgcg ctcatagcga taattatttt ttcgggagca agcccgctct
     3661 gcttgatatc tatcaatcta taataaagta tatctatatc tactaaaaaa tagatatact
     3721 atgatatatt tcgttagaaa tggagcggtt gtggatccgg taagaaaccc ttttgcccct
     3781 ggagcgggca gtcctccacc tgagcttgca gggcggcaag ctattatcga agacgcgcga
     3841 atagccattg accgggcctt gctggggaag cctagccgtt ctcaaatatt cttgggccta
     3901 cgtggtgtag gaaaaaccgt tcttctcaat gcagtcgaaa tgatggcgaa tgagcgcggt
     3961 catttaactt ccagcatcga agcgccagaa aacaagtcac ttggggagct gttgttgccg
     4021 cgcgtgcatc aggtgctgcg taaactttcc cttgccggca acgccaaggc aaaagcccac
     4081 gacgcactgc gcgcgctaag atcttttgct tctgcgttca agtttgaata cggggatatg
     4141 tcgattgccg tggaccctga agtgggagta gcggacagtg gcgacctaga gaacgatctt
     4201 ccagagctat tcgtgcggat cggtgagact gccaaggcag gcgggaaagc atggacgctg
     4261 ctaatcgatg aggtccaata tctcagccaa aaggatttgg ctgcgctcat cgtttcgcta
     4321 cacaagataa atcaaagtaa actgcccgtt ctattttttg gagctggact gccgcaagtc
     4381 gcagcattgt cgggagaggc caagtcatat gcggaaagac tgttccatta tccagaggtt
     4441 ggtgctctgt cagaagcaga tgccaaggaa gcaatccagc aacctgtaag tgaagaaggg
     4501 gctgttatcg atgacgatgc cttgaccgaa atttttcaca aaacaaaggg ctatccctac
     4561 ttcttgcaag agtgggggca tcagtcctgg aatctggcag tcggcaatac cattaccgtt
     4621 caagatgcta agcatgccga gacgaccact ctaaagcgtc tggatgaagg tttttttaag
     4681 gtgcgtttcg atcggctgac acccaaagag cgtgaatacg tcatcgctat ggcgaaatta
     4741 ggtgatggcc catatcgttc ttctgatgtc gccgacgcgc tcaacgaatc cgtacagagc
     4801 ctcgggcccc ggcgtgcgca aactatcagt aaagggatga tttatagtcc ctcgcatggc
     4861 gacattgctt ttactgttcc gatgtttaac gattatctaa tgcgtaattt gactaacctc
     4921 ggttgaatgg cttttgtcca ctaacactgt gaaagctggt agttcggtat atcactcatc
     4981 ggcccgattt catggaacgc gtttctctta atgtaaacag atagccgaca aatgacagca
     5041 tcacgaagat gaacgtgctt atcatgaagt tcgcgtctgg ctggagcagc agccctgaca
     5101 cgaccatggt gccgaccgca gcaaaagaat attggataca ggtcagcagg ccaagtgcat
     5161 gtgagccttt ttcctgaaaa tggttagtga ccataacctg cgcaggtgga ttgatgagcg
     5221 cggtggccag ccgcaacagg caagtgccga gcgtcaccag catgatggtg acaaggacac
     5281 tttgaatcaa tactccagcg acaatgaacg ccgtaccaaa tagtgaggtg taaatgccga
     5341 taccgatgat tttcatggga ggcaaattat tggcgttcaa tttactcatc accctattgc
     5401 ctgacagata tgacaaggca attccaatga agacatagga atagtccgtg ttctcgatac
     5461 cgtattggtc aatgtacaga tagctggaaa taccgatgaa ggcataaaag gctccaaatg
     5521 tcgcccccac aatcagggcc aggcccagaa aatagttgtc cctgaggatt tgataataac
     5581 tcaggaaatg tttttgaaga ctgaacgaag ccctgaagtt ctgctgttgg tggttggttt
     5641 cgccaactgt catctaggcc catacgaaga gcggaatttc aagagcaact atcgcccaga
     5701 agcaattcct ccaggttgag agagccgtaa tccatgctcc gatggcgggc gccagaattg
     5761 gcgatagcac cagcccgatc actagcaacc cggtggcgtg aatttgtact ctcgtatcca
     5821 tcgtatcctt gatgaccagc ctcagtacga tgtatacagt ggcgcttccc aatgcctgga
     5881 cgacacgcat aacgatcatg atcgaaatgg aagaacagag tgcgatccag gccgaggcga
     5941 caatatgcag caacaggcag atgagaagcg ttgcccgtcg cccgtgcaaa tcactgacaa
     6001 ctcccataaa cgatacaaaa aaagcctggc tgtagtgtag accgatatcg tcagattggt
     6061 catgctcatc gttttgccga attccctggc aatcatcggc aaacccgaca ggaatatgtc
     6121 ggtgcaggct tgcgtcgaga ataaaagtag tatcatcaat gaaatagata tttttttact
     6181 gctatctgta ttcatgaacg attcccatgt agacggtgcg tgcataagcg cggaaattac
     6241 tttgcccgat caaacaattc cttctaggtg ccgtaaggtg taatcttggg cgcttgatat
     6301 cgtatactca gtatcccaat gtggttaact ggtgaaaaag ggtatcattc gtatggctgc
     6361 gggcgatgcc gttatggcag atcgcatcga gagctgctat aacgtcagta tattcggcat
     6421 ccgacagatc gtccggctga cattcccgga atgcgatagc aatatctctg agtctgtcgc
     6481 cttggtcacc gatataacaa tccagcccct tattcctgag attgcttgga tttccgtcga
     6541 cgacgatacc gtcgtccctg atgaagtcga tactgaattt tccgatctgt accaatatgt
     6601 agcgtgaatg gctttcgctg tcagagtagt gcgaaacacg atgaatggta tcgtaaggaa
     6661 tttggacgac ttgtccagga taaaaaacat aggatttgct taattgtggt agctctagca
     6721 aaagtcggcc ggctgcgcaa taggtgatgt cggagatatg ggtatgccgg tgataatcca
     6781 gattttcgtg accaacgttg tatacgcaca catatgcatt tccttttatt ggtcgactgc
     6841 gatagtagtg aaaactccgt gagagggaaa gtaactcagc ctgcatctat gctgcctgtt
     6901 gcagtgcgga taggttggtg aagtaaaacg tatccggcgc cttgctgtca agcgtgctgt
     6961 gtacggatgc gacaaggatt catgtgcagc aaatcaaacg ttacgaggcg ggtagctttc
     7021 agtctactgc cgaggcgctt aagaaactgg cgttggtgtt gcacgtgacc acggatttcc
     7081 tgctgtttga ggaaggcgag cgcgaaccgg acgatgattt gacactgcgc ttcgaggcag
     7141 ttgctaccat gccggatgca gaatcagacc ttgagaaaat cttccctgcc gcccagccag
     7201 cgctgcaagt gcgcatcgac gatggcatcg tcgctgtgca ggagttgctg cgccagatcg
     7261 cgcgcctttt ccaccagcca ttgatcggtt gccaggtcgg caaagcgtaa cagggcctgg
     7321 ccggactggc gcgcgccgag aaattcgccg gggccgcgga tttccagatc gcggcgggcg
     7381 atttcaaagc cgtcggtggt ttcgcgcatg gtcatcaggc gttgcctggc agcgccgccg
     7441 agcgggcttt gatatagcag gaggcagacg ctggcggcgc ggccgcgacc gacgcggccg
     7501 cgcagttgat gcagttgcgc cagaccgaaa cgttcggcgt gttcgatcac catcagcgac
     7561 gcgttcggga catcgacgcc gacttcgatc acggtagtgg cgaccagtac gtgcatcccg
     7621 ccacggttga aggcgtccat gacatcctgt ttttcagccg gcttcaggcg gccatgaacc
     7681 aggccgatgt gcaggtcggg cagcgcttcg gccagcatcg cgtgggtagc ggtggcggtt
     7741 tgcagttgca gcgtctcgga ttctttgatc agtggacaga cccagtaaat ctgatggcct
     7801 tcctgtgcgg cggcgtggac gcgcgcgatg acttcggcgc gccggttctg atcgatggtg
     7861 cgcgtgacga cgggcgtgcg gccgggcggc agttcatcga ttaccgagac ttccagatcg
     7921 gcgtaatagg tcatcgccag cgtgcgcgga atcggcgtgg ccgacatcat cagttgatgc
     7981 ggcacgctat tgccggcgcc gaaggatttg ttacgcagcg ccaggcgctg gccgacaccg
     8041 aagcgatgct gttcatcgac gatcaccaga ccgggtttgg cgaactgcac tttgtcttga
     8101 atcagcgcat gcgtgccgat gatcagctgt gctgcgccgg atgcgatgcg cgcactgatt
     8161 tcagcctt
//
LOCUS       DASRDH010000066         2469 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1358032,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000066 DASRDH010000000
VERSION     DASRDH010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1358032"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            385..1446
                     /gene="recA"
                     /locus_tag="VFA14_01640"
     CDS             385..1446
                     /gene="recA"
                     /locus_tag="VFA14_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HZS80120.1"
                     /translation="MDDKKSTHAGNAEKSKALAAALAQIEKQFGKGSVMRMEDGAVVE
                     EVQVVSTGSLGLDIALGVGGLPRGRVIEIYGPESSGKTTLTLQAIAEMQKIGGTCAFI
                     DAEHALDVTYAQKLGIHLPDLLISQPDTGEQALEITDALVRSGGVDLIVVDSVAALTP
                     RAEIEGDMGDSLPGLQARLMSQALRKLTGSINRTNTTVIFINQIRMKIGVMFGNPETT
                     TGGNALKFYASVRLDIRRTGSIKSGDEVIGSETKVKVVKNKVAPPFREAHFDILYGEG
                     TSREGEILDLGSNAKIVEKSGAWYSYNGERIGQGKDNARNYLKERPDLAREIENKVRA
                     FLGVPELAGGAVTPAVAAS"
     gene            1500..1964
                     /gene="recX"
                     /locus_tag="VFA14_01645"
     CDS             1500..1964
                     /gene="recX"
                     /locus_tag="VFA14_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008118508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HZS80121.1"
                     /translation="MRRSPSSLKARALNYLAAREHSRLDLARKLTPYARDGDDVDALL
                     DWLQESGFLSQQRFSESLVQRRAGRYGNDRILSELKHHGIDGEALGEVRRELMQSEVE
                     RACEVLRRKYVEVPADAGTRARCMHFLQQRGFSHHAIRQALRSAWRCAADEG"
     gene            2291..>2469
                     /locus_tag="VFA14_01650"
     CDS             2291..>2469
                     /locus_tag="VFA14_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003812749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HZS80122.1"
                     /translation="MKIHEYQGKEILRRFGVTVPHGIACQSVEDAVKAARTLDGPVWV
                     VKAQIHAGGRGKGGGV"
BASE COUNT          550 a          671 c          720 g          528 t
ORIGIN      
        1 gacgattgtt taccgaacga gccgccgccg ccaagacgcc tggcttcgac gccgccgatg
       61 ccagccgaga gggtcattac tgccacgatc agcgcggcaa atactttttt catgctatct
      121 ccttgaaatg gaatgtaata cgaatacggt cgcgtcgacc gaataatagc gaatagcagg
      181 gagggggcat tgcagttttc cggcagtcga ggcgacagat tataccaacg atcactggat
      241 atgcagccag tcgactaaag tatcaagagc ctgggcgcaa aaatcgtcat ttctcaacat
      301 tgaaaaagag cttgcgcaga caactgtttt ttcatacagt atgccgcagc cattaccacg
      361 aacttttttg ctgaaagaaa ttctatggac gataaaaaat ccacgcatgc cggcaatgcc
      421 gaaaaaagca aggcgcttgc tgctgcgctg gcgcaaatcg aaaagcaatt cggcaagggt
      481 tcggtcatgc gcatggaaga cggcgccgtt gtcgaggaag tccaggtcgt ctcgaccggt
      541 tccctcggtc tggacatcgc attaggcgtt ggtggcctgc cgcgcggtcg agtgatcgaa
      601 atctacggtc cggaatcctc gggtaaaaca accctgacat tgcaagccat tgctgaaatg
      661 cagaaaattg gcggcacatg cgccttcatc gatgcggaac atgcgctgga cgtgacctac
      721 gcccagaaac tgggcatcca tttgcccgac ctgttgatct cccagccgga cacgggcgaa
      781 caggcgctgg aaattaccga tgcgctggta cgttccggcg gcgtcgatct gatcgtggtc
      841 gattccgtgg ccgccttgac gccgcgcgct gaaatcgaag gcgacatggg agactcacta
      901 ccgggcctgc aggcgcgcct gatgtcgcaa gccttgcgca agctgaccgg cagtatcaat
      961 cgtaccaata ccactgtcat tttcatcaac cagattcgta tgaagatcgg cgtcatgttc
     1021 ggcaatccgg aaaccacgac cggtggtaat gcgttgaagt tttatgcctc tgtccgtctg
     1081 gatattcgtc gtaccggttc gatcaagtcc ggcgatgagg tcattggcag cgaaaccaag
     1141 gtcaaggtgg tcaagaacaa agtggcgcca ccattccgcg aagcgcattt cgatattctt
     1201 tatggagagg gtacgtcgcg cgaaggcgaa atcctcgatc tgggttccaa cgccaagatc
     1261 gtcgaaaaat ccggcgcctg gtacagctac aatggcgagc gcattggtca gggcaaggac
     1321 aatgcgcgca attacctgaa agagcgtccc gatctggcgc gcgaaatcga aaacaaggtg
     1381 cgtgcgttcc tgggtgtgcc ggaactggcc ggcggcgcgg tcacaccggc tgtcgcggca
     1441 tcctgaatca tgccgctgtc gaccggcttc gttcgcgcca tggcatttct tctttagcca
     1501 tgcgcagatc gccatcgagc ctgaaagcgc gcgcgctgaa ttatctcgct gcacgcgagc
     1561 atagtcgtct ggatctggcg cgtaagctca ctccttacgc gcgggatggc gacgatgtcg
     1621 acgcattgct cgactggctg caggaatccg gattcctttc ccagcagcgt ttttccgaat
     1681 cgctggtgca gcgtcgcgcc gggcgttacg gcaatgaccg tattctttcc gagctgaagc
     1741 atcatggcat cgatggtgag gcgttgggcg aggtgcggcg cgaactgatg caaagtgaag
     1801 ttgaacgggc ctgcgaagta ttacggcgca agtatgtcga agtaccggca gatgccggaa
     1861 cccgcgccag atgcatgcac ttcctccagc agcgggggtt ttctcatcat gccatccggc
     1921 aggcgctcag gagcgcatgg cgttgcgccg ctgatgaagg ctgagttctg ccgccgttgt
     1981 ctgtccgggg acacgtcatg ctaaaatccg caggtttttt gcgccggcat gaagtggctg
     2041 tcataacgac ttttccatgt acctacgcta cgcaaggcgg cagcgaatgg tgtcgatcaa
     2101 atggcatttt ccatgccgga cgcaccgcat tctctatcag gcaatccgcg aggcgccttc
     2161 gcgcattcat gttcgagcgg gatgcgcaaa cgcggcttcc tgtttccatt cgcgccatgc
     2221 ggtattggcg ccgaccggat ttcaatccag cagggtttat ttaaattgaa tgtcttaaag
     2281 ggaagctccc atgaaaatcc atgagtatca gggcaaagaa atccttcgcc gattcggcgt
     2341 gaccgttccg cacggcattg cgtgtcaatc cgtcgaagat gctgtcaagg cggccaggac
     2401 cttagacggt ccggtctggg tcgtgaaggc gcaaatccac gcgggtggtc gcggcaaggg
     2461 cggcggcgt
//
LOCUS       DASRDH010000067         2519 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_198265,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000067 DASRDH010000000
VERSION     DASRDH010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2519
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198265"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="VFA14_01655"
     CDS             complement(<1..437)
                     /locus_tag="VFA14_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007879586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HZS80123.1"
                     /translation="MNWLRQQHTALAGAFSHLWRSPGNFILNVLVVSIALSLPFAGMT
                     VLENVRPISEQLAVEPEISIFLKMDFPRDKAIALNASISKILQSSGGSAKVIFTPREQ
                     ALGALKNKTGLSEVLATLGTNPLPDSYVLKISGLQSAMDSSGV"
     gene            complement(434..1114)
                     /locus_tag="VFA14_01660"
     CDS             complement(434..1114)
                     /locus_tag="VFA14_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZS80124.1"
                     /translation="MIEFAGVSKQYARDVFALCDITLSVRKGELLFLAGPSGAGKSTL
                     LKMIAAMERPSSGVLSVNSQNINTIRPSGVPYLRRKLGLIFQQQRLLQDRNTLSNVML
                     PLIITGVSRSDAEKRARAALDKVDLLDKSASRPQELSGGEQQRVAIARAIVNRPQIIL
                     ADEPTANLDRDNTNKVLAALKSFHSVGVTCLISTHDEQYLTGADRVVYLDCGRIVSDG
                     VNDLEAAQ"
     gene            complement(1163..2170)
                     /gene="ftsY"
                     /locus_tag="VFA14_01665"
     CDS             complement(1163..2170)
                     /gene="ftsY"
                     /locus_tag="VFA14_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HZS80125.1"
                     /translation="MFSFFKKPPQPESSAIEETAPAPPEEAIEAPPLSSEVRRSWLER
                     LKSGLSKTSGSLTTLFIGAKIDDDLYEELESALLISDAGVEATRFLLDALKKKVKDEK
                     LSEADQVRKALRVLLIDLLRPLQKPLVLGRYQPLVMMVAGVNGSGKTTTIGKLARHMQ
                     AHRQSVLLAAGDTFRAAAREQLAIWGERNNITVIAQESGDPAAVAYDAVHSAQARGTN
                     VVMVDTAGRLPTQLHLMDELKKIKRVIGKSMNTAPHEVLLVIDGNTGQNALAQVKAFD
                     DALGLTGLVVTKLDGTAKGGILAAIAKTRPVPLYFIGMGEQIDDLQAFRAEEFVDALL
                     N"
BASE COUNT          512 a          711 c          747 g          549 t
ORIGIN      
        1 tcgacgccgc tgctgtccat agcgctctga aggcccgaaa ttttcagcac gtagctgtcc
       61 ggcaagggat tcgtacccag cgtcgccaac acctcgctca ggccggtctt gttcttgagc
      121 gcacccaagg cttgttcgcg cggggtgaag atgactttgg cactgccgcc actgctttgc
      181 agaatcttgc tgatgctggc gttcaaggcg atggctttgt cacgcgggaa gtccattttc
      241 aggaagatac tgatttccgg ctcgacggcg agctgctccg atatcgggcg cacgttttcc
      301 agtacggtca tgccggcaaa tggcagcgac agtgcaatcg acaccaccag tacattgaga
      361 atgaagttgc caggcgagcg ccacaggtga ctgaaggcgc cggccaaagc agtgtgctgc
      421 tggcgcaacc agttcattgc gcagcctcca gatcgttgac accatcgctg acgatgcggc
      481 cgcaatcgag atagacgacg cggtcggcgc cggtcagata ttgctcgtcg tgggtcgaaa
      541 tcaagcaggt tacgccgacg ctatggaagg atttgagcgc agccaatact ttgttggtat
      601 tgtcgcggtc gagattggca gtcggttcgt cggccaaaat gatttgcgga cgattgacga
      661 tggcgcgggc gatcgccacg cgctgctgtt cgccgcccga cagttcctgc ggtctggagg
      721 ctgacttgtc gagcaggtcg actttgtcca gagcagcacg ggcgcgtttc tcggcatccg
      781 aacgagagac gccagtgatg atcagcggca gcatcacatt cgatagagtg ttgcgatcct
      841 gcaacagacg ctgttgctga aaaatcaagc ccagcttgcg gcgcaaataa ggcacgccgc
      901 tgggcctgat ggtgttgata ttctggctgt tgacgctgag tacgccgctg ctggggcgct
      961 ccatggcagc gatcattttc agcagggtag acttgccggc accggacggc ccggcgagaa
     1021 agagcagttc acctttccgg accgacagcg tgatgtcaca caaggcgaag acatcacgcg
     1081 cgtactgttt ggagaccccc gcaaattcaa tcatgatcgg tgagttaaat atcctgtttt
     1141 acttttgctt aaacagaatg gctcagttta acaaggcatc gacgaattct tcggcacgga
     1201 aggcttgcag gtcatcgatc tgctcaccca tgccgatgaa gtatagcggt accggacggg
     1261 tcttggcgat ggccgccagg ataccgcctt tggcagtgcc atcgagcttg gtcaccacca
     1321 ggccagtcag gccaagcgca tcgtcgaaag ctttgacctg cgccaacgca ttctggccgg
     1381 tattgccgtc gatgaccagc agtacttcat gcggcgcggt gttcatgctt ttgccaatga
     1441 cgcgcttgat ctttttcagc tcgtccatca ggtgcaattg cgtcggcagc cggccggccg
     1501 tgtcgaccat gaccacattg gtgccgcgcg cctgtgctga atgcacagca tcgtaggcga
     1561 cggctgccgg gtcgccggat tcctgggcga tcacggtgat gttattgcgc tcgccccaga
     1621 ttgccagctg ttcgcgcgcg gcggcgcgga aggtgtcgcc agcagccagc agaaccgatt
     1681 gccggtgcgc ctgcatatgc ctggccagtt tgccgatggt ggtggtcttg ccagagccgt
     1741 tgacgcctgc gaccatcatc actagcggct gatagcgtcc cagcaccagt ggcttttgca
     1801 gcgggcgcag caagtcgatg agcaacacgc gcagagcctt gcggacctga tcagcttcgc
     1861 tcagtttttc gtccttgacc ttctttttca aggcatcgag caaaaaccgg gtggcttcga
     1921 cgccggcgtc cgaaatcagc agcgccgatt ccagctcttc gtacagatca tcgtcgatct
     1981 tggcgccgat gaacagcgtg gtcaggctgc cagaagtctt ggacagaccg ctcttgaggc
     2041 gctccagcca ggagcgcctg acttcggacg acagcggcgg cgcttcaatg gcttcttccg
     2101 ggggcgccgg cgcagtttcc tcgatagcac tggattctgg ctgggggggt tttttgaaga
     2161 aactaaacat cggatgccgg gctgattcgt cgaatgtggc tatgatgccg ggtaagggtg
     2221 cgctacgctg cttccccgaa agaaaaatac aatgaaagag gtacaattga ccggcactgt
     2281 atccagatac tggacactgc tgtccagact gtctttgacc agccccccgc attttaacag
     2341 agcgtagtca atgaaactaa agatgagcgt catcctgtcg gcaggcttac tgttggtctg
     2401 cctgtcggct cgatatcccg cagttcaagc ccagccggtt ccttccgctg ccactgaatt
     2461 catgctaaaa aacggcatga aaatcatcgt cagggaggac catcgcgcgc ccacggtcg
//
LOCUS       DASRDH010000068         3541 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1682717,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000068 DASRDH010000000
VERSION     DASRDH010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 3541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3541
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1682717"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /gene="zapD"
                     /locus_tag="VFA14_01670"
     CDS             <1..513
                     /gene="zapD"
                     /locus_tag="VFA14_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006713184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HZS80126.1"
                     /translation="PDMLNAILGEVDRACVALAASQGKTGQNIRENEWLMGIRGRTII
                     PGGACEFDLPSYYAWQHHSPEQRFNDISNWFAPLVPLFDAVIIVMRLLRESGHAVKIN
                     TNSGSYQQMLQGKVYQMLRLVVDEKLGVTPEISANKYMLWIRFTSQGGDMKPKAFEGE
                     VPFELVLCGF"
     gene            530..715
                     /locus_tag="VFA14_01675"
     CDS             530..715
                     /locus_tag="VFA14_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HZS80127.1"
                     /translation="MATIVNCPSCGARVEWAEKNAFRPFCSERCKQIDLGAWAEEKYV
                     IPAVNLPEQNKNDPATD"
     gene            complement(740..1159)
                     /locus_tag="VFA14_01680"
     CDS             complement(740..1159)
                     /locus_tag="VFA14_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HZS80128.1"
                     /translation="MSIAPPTTAPPIDVAVGILIKPNGDVLLGQRPEGKPYAGHWEFP
                     GGKVESGESIVDALKREFLEELGIEIISAEPWCGMQYVYPHAHVRLHFYFSRKWRGEP
                     QSLEGQAFAWQGSIEVGPLLPATLPLIEWIDTLRIPV"
     gene            complement(1159..2040)
                     /locus_tag="VFA14_01685"
     CDS             complement(1159..2040)
                     /locus_tag="VFA14_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZS80129.1"
                     /translation="MTQLDQFLQRAEALLSRIESILPQSAAAVEWNKCLAFRWRRRDG
                     SGNGGGYLQSVGHISNIALSDLHGIGPQKEQIDRNTRQFVDGRPANNVLLTGARGTGK
                     SSLIKACLNQYADRGLRLIEVDKDDLHDLPNIGDLVSARPERFIIFCDDLSFEEGENG
                     YKALKVALDGSIAAQSDNVLIYATSNRRHLMPERMSDNLTYTHTDDGDLHPGETVEEK
                     ISLSERFGLWVTFYPFKQDDYLDIVAHWLRHFGCNAAQIEEARGDALRWALQRSSRSG
                     RVAWQFSRDYAGKLQTR"
     gene            complement(2089..3324)
                     /gene="argJ"
                     /locus_tag="VFA14_01690"
     CDS             complement(2089..3324)
                     /gene="argJ"
                     /locus_tag="VFA14_01690"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008334569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HZS80130.1"
                     /translation="MAVNSPLPVSADLHPVAGIELGHAEAGVCKTSRKDLLVMKLAPT
                     ATVAGVFTRNRFCAAPVQVCRMHLAQAGVAAPIRALVINTGNANAGTGAEGLARAHAT
                     CTALATLLGVKPEQILPFSTGVILEPLPLDRIEAGLPVAVANLKTDNWFNAAESIMTT
                     DTQPKAVSRTLIIGGKQVVMTGISKGAGMIKPNMATMLGFLAFDAKVPQILLNQLVKD
                     AADRSFNCITIDGDTSTNDSFMLIATGAGTLEIASADSAEYRELAAAVYALSQNLAHQ
                     IVRDGEGATKFIEVTVEDGKSVEECRRIAYAIGHSPLVKTAFFASDPNLGRILAAIGH
                     AGIDDLDVSTLNLYLDDVWVARNGGRHPDYREEDGQRVMQKSEIVVCVKLARGTAKAS
                     VWTCDLSHEYVTINADYRS"
     gene            complement(3353..>3541)
                     /locus_tag="VFA14_01695"
     CDS             complement(3353..>3541)
                     /locus_tag="VFA14_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010399224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family transporter"
                     /protein_id="HZS80131.1"
                     /translation="LLALAVKTLPLSTAYAIWTGIGAAGAVILGIVLFHEPASLARLA
                     CLALIIVGIIGLKIVSPE"
BASE COUNT          782 a         1015 c         1002 g          742 t
ORIGIN      
        1 cctgacatgc tcaatgccat cctcggagaa gtcgatcgcg cctgtgtggc attggctgct
       61 tcgcagggca agaccgggca gaatattcgc gaaaacgaat ggctcatggg tatccgtggc
      121 cgtaccatca tcccgggcgg cgcctgtgaa tttgatctgc catcctatta cgcatggcag
      181 caccattcgc cggagcagcg tttcaacgat atttccaact ggttcgcacc gttggtgccg
      241 ctgttcgacg ccgttatcat cgtgatgcgt ctgttacgcg aatccggtca tgccgtcaaa
      301 atcaatacca atagcggcag ctaccagcaa atgctgcagg ggaaagtcta ccaaatgctg
      361 cgtctggtgg tggacgagaa actgggtgtc actcctgaga tttcagccaa taaatacatg
      421 ctttggattc gcttcacctc acagggtggt gatatgaaac cgaaggcgtt cgaaggcgaa
      481 gtgccgtttg aactggtgct ttgcggattt tgaagtagag gtagaaatca tggcaacgat
      541 cgtcaactgt ccaagctgcg gcgccagagt ggaatgggca gaaaagaatg cattccgtcc
      601 gttttgctcg gaacgctgca aacagatcga tctcggcgcc tgggctgaag aaaaatacgt
      661 gattcccgca gtcaacctgc cggagcagaa taaaaacgat cctgcaacag attgagtcgg
      721 tgcgccgcct ggagcgcgtt tacactggta tgcgcagtgt atcgatccac tcgatcagcg
      781 gtagcgtcgc cggcaacagc ggtccgactt caatgctgcc ctgccaggcg aatgcctgcc
      841 cttccagact ttgcggttcg ccgcgccatt tccggctgaa gtaaaaatgc aaacgcacat
      901 gcgcgtgcgg atagacgtac tgcatgccgc accatggttc ggccgagatg atctcgatac
      961 ccagttcttc cagaaactcg cgcttgagcg catccacaat cgactctcca gactcgacct
     1021 taccgccggg gaattcccaa tgaccagcat acggtttgcc ctcgggacgc tggcccagta
     1081 gaacatcgcc gttcggtttt atcagaatcc cgacggcgac atcgatcggc ggcgccgttg
     1141 ttggcggggc aatggacatc atcgggtttg cagctttccg gcgtagtccc tggagaattg
     1201 ccaggccaca cgcccggagc gcgaactgcg ttgcaaagcc cagcgtaagg catcgccgcg
     1261 cgcctcttcg atctgtgccg cgttgcagcc gaaatggcgc aaccagtgcg caacaatgtc
     1321 gagataatcg tcctgcttga acgggtagaa agtcacccac agcccgaagc gctcggacag
     1381 ggaaatcttc tcctccacgg tctcgcccgg atgcaggtcg ccatcatcgg tatgtgtata
     1441 cgtcaaatta tccgacatgc gctccggcat cagatgacga cggttggagg tcgcataaat
     1501 cagcacattg tccgactgcg cagcgatact gccatccagc gctactttga gcgccttgta
     1561 cccattctcg ccttcttcaa aggataaatc gtcgcagaag atgatgaagc gctcaggacg
     1621 cgccgatacc agatcgccga tgtttggcaa gtcatgcagg tcatccttgt ccacctcgat
     1681 cagacgcaga ccacggtcgg cgtattgatt caggcatgcc ttgatcagcg aggacttgcc
     1741 ggtaccgcgc gcaccggtca acagtacgtt gttggctgga cggccgtcca cgaattgcct
     1801 ggtattccgg tcgatctgtt ctttctgcgg accgatacca tgcagatccg acagcgcaat
     1861 gttcgagata tgcccgaccg attgcaggta gccgccgcca ttaccgctac cgtcgcgacg
     1921 gcgccagcgg aacgccaggc acttgttcca ttccactgcg gcagccgact gcggcaagat
     1981 ggattcgatg cgcgacagca aggcctcggc gcgctgaaga aactgatcta attgagtcat
     2041 gacaaatcag caaagtggat tgatacgtcg tatcgtgaca ggaaatgctc acgaacggta
     2101 atcggcattg atggtgacgt attcatggga cagatcgcaa gtccagaccg aagctttggc
     2161 agtgccgcgc gccaatttca cacacaccac gatttcgctc ttctgcatga cgcgctgacc
     2221 atcttcttcc ctgtaatcgg gatggcgacc gccgttcctg gcaacccaga catcgtccag
     2281 atacagattc agtgtcgaga catccagatc atcgataccg gcatggccga tcgccgccag
     2341 gatacggccc agattcggat cggaggcgaa gaaagcggtt ttgaccaatg gcgaatgacc
     2401 aatggcataa gcgatcctgc gacattcttc aacgctcttg ccgtcttcca cggtgacttc
     2461 gatgaatttg gtagcacctt cgccgtcgcg cacgatctgg tgcgcgaggt tttgcgacaa
     2521 agcataaacg gcagcggcca gttccctgta ttcggcgctg tcggcgctgg cgatttccag
     2581 tgtgccggcg ccggtggcaa tcagcataaa ggaatcattg gtcgaggtgt cgccatcgat
     2641 ggtgatgcaa ttgaaggatc gatcggcagc atccttgact agctggttca gcagtatttg
     2701 cggcactttg gcgtcgaacg ccagaaaacc cagcatggtc gccatgttcg gcttgatcat
     2761 gccggctcct ttgctgatgc cagtcatgac gacctgtttg ccgccgatga tcaatgtgcg
     2821 cgagactgct ttcggctgcg tgtcggtggt catgatggac tcggccgcat tgaaccagtt
     2881 atcggttttc agattcgcga cagcgaccgg caggccagcc tcgatacgat cgagcggcag
     2941 tggttccagg atgacaccgg tcgagaatgg caggatttgt tcgggcttca cgcccagcag
     3001 cgtcgccagt gcagtgcagg tcgcatgtgc gcgcgccaga ccctctgcgc cagtgccggc
     3061 gttggcgttg ccggtgttga tcaccagcgc gcgaatcggc gccgccacgc cagcttgtgc
     3121 caaatgcatc ctgcacacct gcaccggagc ggcgcagaaa cggttgcgtg tgaacacgcc
     3181 ggcaaccgtt gccgtcggcg ccagtttcat gaccagcaag tccttgcggc tggttttaca
     3241 cacgccggct tcggcatggc ccagttcgat gccggcaaca ggatgcaaat cggcagaaac
     3301 cggaagggga gaattgacag ccatgatcat tcagccagag ggagcgatgc tgtcattcgg
     3361 gagacacgat tttcagaccg atgattccga cgatgatcaa cgctagacaa gccagtctgg
     3421 ccagcgaggc cggttcatga aacaggacga taccgagaat caccgcaccg gcagccccga
     3481 tgccagtcca gatcgcatat gccgtactga gcggcaaggt tttcaccgcc agcgccagca
     3541 a
//
LOCUS       DASRDH010000069         3687 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_784143,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000069 DASRDH010000000
VERSION     DASRDH010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 3687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3687
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_784143"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="VFA14_01700"
     CDS             <1..612
                     /locus_tag="VFA14_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008116324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HZS80132.1"
                     /translation="HYTCANWGRDLPEPMVADIRNVVEGDVSPDGKGVLAIQRGIEVG
                     HVFQLGTTYSESMKATYLDETGKPQPLQMGCYGIGITRILGSAIEQNCDDKGIIWPTA
                     LAPFEVVLCPLGYDRSEAVRTEADKLYDALLAIGIDVILDDRGERPGAMFADWELIGV
                     PHRIVIGERGLKSDQLEYQGRRDATATPVPLADALVFIQGKLA"
     gene            complement(977..1342)
                     /locus_tag="VFA14_01705"
     CDS             complement(977..1342)
                     /locus_tag="VFA14_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007878048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIB/MDM2 domain-containing protein"
                     /protein_id="HZS80133.1"
                     /translation="MSTARKPVVAAKKSAAKAVPKKAVAAPKKAAPKQAATKRVPNPT
                     FMKPLTPSAALAAVVGDKPLPRTEVTKKVWEYIKKHKLQNPENKRNIDADDKLEVVFG
                     YKKQVSMFEMTKLISGHLK"
     gene            complement(1540..2043)
                     /locus_tag="VFA14_01710"
     CDS             complement(1540..2043)
                     /locus_tag="VFA14_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS80134.1"
                     /translation="MRKKGVFLNIIHRVTLAYALLAPGMPGIASAAPPAFGIEIGNAL
                     LCLNQLNSKYFYDYLFEAFGKPHKNEGGAYWFKSADSTLWGMEVTDVLVNNSSSPAEF
                     VGAVVKGTPQELADAIGKSVGIHYTLEDNGQYAQRRSQSGSVIAYADSKAKIYCAKSK
                     LLMPGNQ"
     gene            2253..3620
                     /gene="purB"
                     /locus_tag="VFA14_01715"
     CDS             2253..3620
                     /gene="purB"
                     /locus_tag="VFA14_01715"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HZS80135.1"
                     /translation="MSFTSLSALSPLDGRYAAKTDKLRPILSEIGFMHHRVKVEVAWL
                     QALSVAGFAEIKPFSAAATALLEALVADFTETDAARIKEIEAVTNHDVKAVEYWLKEK
                     ISGVPELAAASEFIHFACTSEDINNTSYGMMLKTARDTVLIPALQSLIARLTGLAHEH
                     AAVPMISRTHGQLASPTTLGKEIANVVARLQRAVKRIAAVDILGKMNGAVGNYNAHLS
                     AYPETDWVAFSRHVIEQRLGLVFNPYTTQIEPHDYMAELFDAVARANTVLLDLNRDIW
                     GYISLGYFRQRTKAGEIGSSTMPHKVNPIDFENSEGNLGMANTVLKHLSEKLPVSRWQ
                     RDLTDSTVLRNIGVAFGHAVLAYDSCLRGLNKLEVNPARLAEDLDTAWEVLAEPVQTV
                     MRRYGIANPYEQLKELTRGKGISAEALHDFIDGLAIPQNAKDHLLAMTPANYIGAAIA
                     LASNI"
BASE COUNT          805 a         1023 c          987 g          872 t
ORIGIN      
        1 cactatacct gtgccaactg ggggcgcgat ttgccagagc caatggtggc cgacatccgc
       61 aacgtcgtcg aaggtgatgt ttctccggat ggcaagggcg tgctggcgat ccagcgcggt
      121 atcgaagtcg gccatgtgtt ccagctcggt accacctatt cggaatcgat gaaagcgact
      181 taccttgacg aaaccggcaa gccgcaaccg ctgcaaatgg gctgttatgg cattggcatc
      241 acccgcatct tgggatccgc catcgaacaa aattgcgacg acaagggcat catctggccg
      301 actgcgttgg cgccgttcga agtggtcctg tgtccgctgg gttacgaccg cagtgaagcc
      361 gtcaggaccg aagccgacaa gctctacgac gccttgctgg ccatcggtat cgatgtcatt
      421 ctggatgacc gcggcgaacg tcctggcgcc atgtttgcgg attgggaact gatcggcgtg
      481 ccgcatcgca tcgtcatcgg agagcgtggc ttgaagagtg atcagcttga ataccagggc
      541 cgccgcgatg cgactgcaac accggtgcca ctggctgatg cactggtttt catccagggc
      601 aagctggcct gatttgaggc ttggcgcttt ggcaacctcc tgacggagac attcgcgcag
      661 aatgaaaaaa ggtccatgcc gactgccatg atggatccca gcttgttgtg ggtcaccgag
      721 gagtacgtac acgaactgct cttcaggaaa gtagtaatgt atcgacagcg attgaaataa
      781 tcaaaaaaat tcaaatattc tgtcgcgaat tctttatatg ccactctact acatgactga
      841 atcaaactca tgttaaaagg cttgatcccg gtaccatacc gggatcaagc cttttctatt
      901 tagcccgact tcttggggtc agttcacggg acggaggttt ctgttttaca agccagtcta
      961 cgaaaatctg tattgcttat ttcaaatggc cggaaatcag cttggtcatt tcaaacatcg
     1021 atacttgctt cttgtagcca aatacgactt ccagtttatc gtctgcgtcg atattgcgtt
     1081 tgttttcagg gttttgcagt ttgtgtttct tgatatattc ccacaccttc ttggtcactt
     1141 cggtacgcgg cagaggtttg tcaccgacga ctgcagcaag tgctgcagat ggggtcagcg
     1201 gcttcatgaa tgtgggattg ggcacacgtt tggtagctgc ctgctttggc gcagcttttt
     1261 ttggtgccgc aactgctttc tttggaacgg cttttgctgc ggacttcttc gctgcgacta
     1321 ctggttttct ggctgtactc atcggtagcc tccttgacaa agaaaaaatt gaatatataa
     1381 attacgcaag accaatagtg gtgtggtttt cccggcgatg caagtatttt ttggcacttt
     1441 tcagagagaa aacgtacaac aaaccggttt cggctcattt tcgaacgctt gcagtgtgac
     1501 aggcgctgga gataattggc gatagtcacg gcacgtcgtc tattgatttc caggcatcag
     1561 caatttggat ttggcgcagt agatttttgc cttggaatcg gcataagcga tgacactgcc
     1621 ggattgcgac cggcgttgcg cgtattgacc gttgtcttca agcgtgtaat ggataccgac
     1681 gcttttgccg atggcgtcgg ccagctcttg cggcgtgcct ttgaccacgg cgccgacgaa
     1741 ttcggctggg ctgctgctat tgttgaccag cacgtcggtg acttccatgc cccagagggt
     1801 gctgtcagca gacttgaacc agtaggcgcc gccttcattt ttatgcggct tgccgaaggc
     1861 ctcgaagaga tagtcgtaga agtatttgct attcagctga tttaggcaca gtaaggcgtt
     1921 gccgatttcg atgccaaacg caggcggggc ggcgctggca atgccgggca tgcccggtgc
     1981 cagcagtgcg taggccagcg tgacgcggtg aatgatgttc aaaaagactc ccttcttgcg
     2041 catggcaatg ttgattgctg ctaaaggtaa gatgcgagga ttgtaggggg cttttctttt
     2101 tttgccaatt ccatgtcccg gacatgcggc atgaagtctt gccgcagtgg taaaatcgtc
     2161 aactcatata cgtctgctcc ggctgcaacc ttccctgacg cagcgcggct ctttgctctt
     2221 gttttcagcg ttgtcatcat ccgcatatca gcatgtcttt tacctcgctt tccgctctct
     2281 ccccgctcga cggccgttat gccgccaaaa ccgacaagct gcgcccgatt ctgtccgaga
     2341 taggtttcat gcatcaccgg gtgaaagtgg aggtcgcctg gttgcaggcc ctgtcagttg
     2401 ccggctttgc cgagataaaa ccattctcgg ccgcagctac cgccttgctt gaggcgctgg
     2461 tggctgactt taccgaaacc gacgccgccc gcatcaagga aatcgaagcc gtgaccaacc
     2521 acgacgtcaa ggccgtggaa tactggctca aggaaaaaat cagcggcgtg ccggaactgg
     2581 ccgctgcctc cgaattcatc catttcgcct gcacttcgga agacatcaac aatacttcct
     2641 acggcatgat gctcaaaact gcgcgcgaca ccgtgctcat ccccgccctg caaagcctga
     2701 tcgccaggct aaccggactg gcgcatgaac acgccgccgt gccgatgata tcgcgcaccc
     2761 acggccagct ggccagcccc accacgctag gcaaggaaat cgccaacgtc gtcgcgcgcc
     2821 tacaacgcgc cgtcaagcgc atcgccgcag tcgacattct cggcaagatg aatggagcag
     2881 tcggcaacta caacgcacac ttgtccgcct acccggaaac cgattgggtc gcgttttcca
     2941 ggcatgtcat cgaacagcgc ctgggtctgg tattcaatcc ctacaccacc cagattgaac
     3001 cgcatgacta tatggctgaa ctgttcgacg ccgtagcgcg tgctaacacg gttctgctcg
     3061 atctgaaccg cgacatctgg ggctacatct cgctgggcta tttcaggcaa cgtaccaaag
     3121 ctggagaaat cggttcgtcg accatgccgc acaaggtcaa cccgatcgac ttcgaaaact
     3181 ccgaaggtaa tctgggcatg gccaataccg tactcaaaca tctgtcggaa aaacttcctg
     3241 tctcccgctg gcagcgtgac ctgaccgatt cgaccgtgct gcgcaatatc ggcgtcgcct
     3301 tcggccatgc cgtgctggcc tatgacagtt gcctgcgcgg cctgaacaaa ctggaagtca
     3361 atccggcgcg cttggccgaa gatctggata ccgcctggga agtgctggcc gagccggtac
     3421 aaaccgtgat gcgccgctac ggcatcgcaa atccatacga gcagctcaag gaactgactc
     3481 gtggcaaagg catttccgcg gaagcattgc acgactttat cgacggactg gccattccgc
     3541 aaaatgccaa ggatcacttg ctggcgatga cacctgccaa ctacatcggc gcagcgattg
     3601 cactggccag caatatttga catcgatgcc ggatcgagtg gaactactgc cactgccact
     3661 gccactgcca ctgccactgc cactgcc
//
LOCUS       DASRDH010000070         2301 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_879856,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000070 DASRDH010000000
VERSION     DASRDH010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_879856"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /gene="ribH"
                     /locus_tag="VFA14_01720"
     CDS             <1..422
                     /gene="ribH"
                     /locus_tag="VFA14_01720"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HZS80136.1"
                     /translation="RFNEDVCHGLLAACLAELKHLGVQDEDILHVSVPGALEIPLALQ
                     KMADSVQFDALIALGAVIRGETYHFELVSNESGAGIARVSLDASIPIANAVLTTESDE
                     QAEARMEVKGADAARVAVEMANLALALNELAQVTEEE"
     gene            453..920
                     /gene="nusB"
                     /locus_tag="VFA14_01725"
     CDS             453..920
                     /gene="nusB"
                     /locus_tag="VFA14_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016836166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HZS80137.1"
                     /translation="MTNKTQHANPSKNRTPRHRAREFALQGLYQWLLNNEDAGVIEAH
                     IREAHGFDKADAEHFDVLLYGAIKQAADLRAVLVPLIDRPITELSPVEHAVLLIGSFE
                     LKHSIEVPYKVVINEAVELTKSFGGVDGHKYVNGVLDRLAAKVRDTEAGAGRA"
     gene            complement(1085..1369)
                     /locus_tag="VFA14_01730"
     CDS             complement(1085..1369)
                     /locus_tag="VFA14_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007880659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HZS80138.1"
                     /translation="MNKSELVDAIAADTEISKATAQRALDSVAENIIKAVAKGGTVQL
                     VGFGSFSTGKRAARTGRNPQTGEAIKIAAATTVKFSAGKSFKEAVNKKKK"
     gene            1754..2182
                     /gene="mraZ"
                     /locus_tag="VFA14_01735"
     CDS             1754..2182
                     /gene="mraZ"
                     /locus_tag="VFA14_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008119764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HZS80139.1"
                     /translation="MFQGASSIHLDAKGRMSIPARHRDALVLQCEGRVTLTRHPHGCL
                     LFFPRPTWESHREQIAAWPMSARAWQRIFLGNAFDVEMDSAGRILIAPELRAAVSLAR
                     DVMLLGMGSHFEIWDAARLVENESEAVAAGMPDVLSNFSF"
     gene            2195..>2301
                     /locus_tag="VFA14_01740"
     CDS             2195..>2301
                     /locus_tag="VFA14_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytosine(1402)-N(4))-methyltransferase"
                     /protein_id="HZS80140.1"
                     /translation="MTLQSVPGLQHRTVLLEEAVDALAIEGVRADGIYL"
BASE COUNT          500 a          561 c          666 g          574 t
ORIGIN      
        1 cgcgtttcaa tgaagatgtg tgccacggtt tgctggcggc ctgtctggcg gagctgaaac
       61 atttgggcgt gcaggatgaa gatatcctgc acgtgagcgt gccgggtgcg ctggaaattc
      121 cgttggcctt gcagaaaatg gccgacagtg tccagttcga tgcgctcatt gcactgggtg
      181 ctgtgattcg cggcgagacg taccacttcg aactggtatc gaacgaatct ggcgccggca
      241 ttgcgcgcgt cagcctcgac gccagcattc ccatcgccaa tgctgtactg accacggaga
      301 gcgacgagca ggcggaagcg cgtatggaag tcaagggcgc ggatgcggcg cgcgtggcgg
      361 tcgaaatggc caatctggcg ctggcgctga atgagttggc gcaagtaacg gaagaggaat
      421 aacggtatga accgtttcac cctgtatttg acatgactaa taaaacccaa cacgccaatc
      481 cgagcaagaa ccgcacgccg cgtcaccgcg cgcgtgaatt tgccctgcag ggtctgtatc
      541 agtggctgct caacaatgaa gatgctggcg tcatcgaagc acacatccgc gaagcgcatg
      601 gtttcgacaa agccgacgcc gaacatttcg acgtgctgct gtatggcgcc attaagcagg
      661 ccgccgattt gcgtgcggtc cttgtgccgc tgatcgatcg tcccatcact gaactgtcgc
      721 cggtggaaca tgccgtcttg ttgattggct ctttcgaact aaagcacagc atcgaggttc
      781 cctataaagt cgtcatcaat gaagcagtcg agctgaccaa atccttcggt ggtgtcgatg
      841 gacacaagta tgtcaacggc gtgcttgatc ggttggccgc caaagtgcgc gacacagagg
      901 caggtgcagg acgcgcctga ccttttcatt gcgaggccgc tgtagaagcg caccaaaaag
      961 atttgcggtt gaataccggg aaagcgtgaa gggcgttgcg aaaggtatgc gctgaagtat
     1021 tgacctggaa cacggtgttt gaatgctgtg aactccctgg tccacggcat tcaaaattgc
     1081 atcatcattt tttctttttg ttgacggctt ctttgaacga cttgccagcg gagaatttca
     1141 ctgtcgttgc cgcagcgatt ttgattgcct cgccagtttg aggattacgg ccggtacggg
     1201 cagcgcgctt gcctgtagag aaagaaccga agccaaccag ttgcacggtg ccgcccttgg
     1261 ccactgcttt gatgatgttt tcagcaacgg agtccagagc gcgttgcgcg gtagccttcg
     1321 agatttcagt atcagcagcg atcgcatcga ctaattcgga tttattcacg ttgaaattcc
     1381 tttcactgtt aaggtgcgga gattttagca ttgtaatgtg ttcctgccac aagtcccgtc
     1441 tgatgttgtc actgatttca tgggtagcag gagataaaca catgtgtata tctcaagtga
     1501 aatattcaat atgcttctca aaccattcat ttttaggaca aagcactttg tgacgttttc
     1561 tgattaagtg cgctaagtct ttgactttat tgcaaaaatt cctttttttc catgaatatc
     1621 gcttgacctg tattagtgca tcctctaaag tggtgaaaag tgtaaaaaag tgtttttttg
     1681 tgggacatgc ttcccacata aggacattgc atttataacc atgcaccagc gacaggggaa
     1741 aaggtcaagc atagtgtttc agggcgcttc atccattcat cttgatgcca aaggacggat
     1801 gtctattccg gccaggcatc gtgacgccct ggtgctgcag tgcgaaggac gggtcacgct
     1861 gacgcgtcat ccgcacggct gtctgttgtt tttcccgcgt ccgacctggg agagccatcg
     1921 cgagcagatc gccgcctggc cgatgtcggc gcgcgcctgg caacggattt ttctgggcaa
     1981 tgctttcgat gtcgagatgg attccgctgg tcgcatcctg atcgcgccgg agttgcgcgc
     2041 agcggtgagt ttggcgcgcg atgtcatgtt gctgggcatg ggcagccatt ttgaaatctg
     2101 ggatgctgcc agactggtcg agaatgaatc ggaagccgtg gcggccggca tgccggatgt
     2161 attgagtaat ttttccttct gacggcacga cgcgatgact ttgcaatcgg tgcctggttt
     2221 gcagcaccgt acggtgctgt tggaggaagc cgtggatgct ctggcgattg agggcgtgcg
     2281 cgctgacggt atttatctgg a
//
LOCUS       DASRDH010000071         4442 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_108726,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000071 DASRDH010000000
VERSION     DASRDH010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 4442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4442
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108726"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..1517)
                     /gene="mnmE"
                     /locus_tag="VFA14_01745"
     CDS             complement(111..1517)
                     /gene="mnmE"
                     /locus_tag="VFA14_01745"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006461364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HZS80141.1"
                     /translation="MTPDPSPIAAVATASGRGGIGVVRISGKNLWPVVQAVCGKDEGA
                     LPPRHATYLPFKRADGSVIDRGLAIYFKAPHSYTGEDVLELQGHGGPVVMRMLLARCL
                     EAGAGIGLRLAEPGEFTQRAFLNDKLDLAQAEAVADLIEASTEAAARSAAASLSGVFS
                     RVIHDLVGKVTNLRMLVEATLDFPEEEIDFLEHSDARAQLFAIRAALEDVFTHASHST
                     LLRDGLHVVLAGKPNVGKSSLLNALAGSDVAIVTPIAGTTRDKIIETIQINGIPLDII
                     DTAGIRNVDAYGSGPDEVERIGIERTWDAVAQADVILHMLDADCGPTREDEKITERFP
                     AGIPVIRIWNKIDRSGHKPAVDHLPDATHIYLSATSHIGIDLLRNELLRIAGGQQTLE
                     SPYLARERHLIALRDARDYLANAALHAEADKQTGDKSIDLFAEELRLAQERLSSITGK
                     FTPDDLLGMIFSRFCIGK"
     gene            complement(1557..3188)
                     /gene="yidC"
                     /locus_tag="VFA14_01750"
     CDS             complement(1557..3188)
                     /gene="yidC"
                     /locus_tag="VFA14_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017449788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HZS80142.1"
                     /translation="MDIQRTILWIVFAFSLLFLWDSWQRHDGKPSMFFPMTAQPAADA
                     ADPSTSPVPQATAAATDASVAVAPGAKSETITIMTDVVKADVNTVGGELKRLELLKHR
                     DTVDPARNLVLFDASASHTYLAESGLIGGPFPNHKSVFTAKPGARVLDGSNEVQLVLE
                     AQENGVKLTKTYTFQRDAYVIDLKHTVTNNSANAIDPSLYLQLLRDGNKPDGESYFYS
                     TFTGPAVYTDADKFQKLAFEKIAEGKQEHATKSDNGWIALVQHYFVSAFIPPEKAQRD
                     IFAKKVAANLYAVGNILPLGTVAPGASVTMDVRLYSGPQESKVLEATAPGLELVKDYG
                     WLTIIAKPIFWLMMYIYAFVGNWGWTIILLTLLIKLMFFPLSAASYRSMAKMKAVTPK
                     MTAIRERHQGDPQAMNREMMTLYKTEKINPLGGCLPIVIQIPVFISLFWVLLASVEMR
                     GAPWLGWIHDLTAPDPFYILPVMMAASMFIQTKLNPTPPDPLQAKIMMFMPIAFSVMF
                     FFFPSGLVLYWVVNNVLSIAQQWLITRNLEKGQAK"
     gene            complement(3208..3462)
                     /gene="yidD"
                     /locus_tag="VFA14_01755"
     CDS             complement(3208..3462)
                     /gene="yidD"
                     /locus_tag="VFA14_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HZS80143.1"
                     /translation="MKTPLLLLLHAYKLGISPYLGQNCRFYPSCSDYAAEAIRRHGAW
                     KGSALAGKRLCRCHPWHAGGFDPVPDSSSPTATADGCGYS"
     gene            complement(3462..3866)
                     /gene="rnpA"
                     /locus_tag="VFA14_01760"
     CDS             complement(3462..3866)
                     /gene="rnpA"
                     /locus_tag="VFA14_01760"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013236262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HZS80144.1"
                     /translation="MTDDHSHLYSRDRRIVKTDEFSSVFRLRPVYRSAHFILYARNND
                     LPLARLGVITAKRMAPRAVTRNMIKRIIRESFRQAALPQVDCIVRLFSPVNSRSEPAS
                     TAKLKTALRAELLQLFTFLLNAPAFAGKSGNK"
     gene            complement(3898..4035)
                     /gene="rpmH"
                     /locus_tag="VFA14_01765"
     CDS             complement(3898..4035)
                     /gene="rpmH"
                     /locus_tag="VFA14_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003816025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HZS80145.1"
                     /translation="MKRTYQPSVVRRKRTHGFRARMATRAGRAVINARRAKGRKRLAV
                     A"
BASE COUNT          955 a         1175 c         1343 g          969 t
ORIGIN      
        1 tgtgagcggc tgtgactgta tcaccgaatc cgtcagtaga caaaaatccc tgtagctatg
       61 cggcatctgg ctaaatcagg aaacgccttt ggatctcagt ggacatcgaa tcacttcccg
      121 atgcagaagc gtgaaaaaat catccccagc aaatcgtccg gcgtgaattt gccggtgatg
      181 ctggagagac gctcctgcgc caggcgcaat tcttccgcaa acagatcgat agacttgtcg
      241 ccggtctgtt tgtcggcctc ggcgtggagg gcggcattcg ccagatagtc acgcgcatcc
      301 ctcagggcaa tcaggtggcg ctcgcgcgcg agataaggtg attccagcgt ttgctgccca
      361 cccgcgatgc gcaacagttc attgcgcaat aaatcgatgc cgatgtgact ggtggctgac
      421 aggtagatat gggtggcgtc gggcagatga tcgacggcgg gtttgtggcc ggagcggtcg
      481 atcttgttcc agatgcggat gacgggaatg cctgccggaa aacgctcggt gattttttca
      541 tcctcccgtg ttggcccgca gtcggcatcg agcatgtgca gaatcacgtc ggcttgcgcg
      601 actgcatccc aggtacgctc gatgccgatg cgttcgactt cgtcggggcc gctgccgtat
      661 gcatcgacgt tacggatgcc ggcggtgtcg atgatgtcga gcgggatgcc gttgatctga
      721 atggtttcga tgattttatc gcgtgtagtg ccggcgatcg gtgtgacgat ggcgacatcg
      781 gaaccagcca gcgcgttcag cagcgacgat ttgccaacgt tgggtttgcc ggccagcacg
      841 acatggaggc catcgcgcag caaggtgcta tgcgatgcat gcgtgaatac gtcctccagc
      901 gcggcacgaa tggcaaagag ctgagcgcgt gcgtcggagt gttcgagaaa atcaatttct
      961 tcttccggga aatccagggt cgcttcaacc agcatacgca gattggtgac cttgccgacc
     1021 agatcatgaa tgaccctgga gaagacgccc gacagcgatg cggcagccga cctggcggcg
     1081 gcttcggtcg atgcttcgat caggtcggcg acggcctcgg cttgtgcgag atcgagcttg
     1141 tcgttgagaa aagcgcgttg ggtgaattcg cccggttctg ccaggcgcag gccaatacca
     1201 gcgccggctt cgaggcaacg cgccaacagc atgcgcatga cgacaggacc gccatggcct
     1261 tgcagttcaa gcacgtcttc gccggtatag gaatgcggcg ccttgaaata aatcgccaga
     1321 cctcgatcga tcacgctgcc atcggcgcgt ttgaacggca gataggtagc atggcgcggc
     1381 ggtagcgcac cttcatcctt gccgcagaca gcctggacta cgggccatag attcttgccg
     1441 gaaatgcgga ccacgccaat gccaccgcgt ccggatgcgg tggcgacagc agcgataggt
     1501 gatggatcgg gtgtcatgga gcgcaacgat tttttgcagc agattcagaa aagattttac
     1561 ttcgcctggc ccttttccag gttcctggtg atgagccatt gttgcgcgat cgatagcacg
     1621 ttgttcacga cccaatacag cacgaggccg gacgggaaga agaagaacat gaccgagaac
     1681 gcgattggca tgaacatcat gatcttggcc tgcaacggat ccggaggcgt cgggttgagc
     1741 ttggtctgga tgaacatcga cgccgccatc atgaccggca ggatataaaa cgggtcgggc
     1801 gcggtcagat catggatcca gcccagccat ggcgcgccgc gcatttcaac gctggccagc
     1861 aacacccaga acagcgagat gaataccgga atctggatca cgatcggcag gcaaccgccc
     1921 agcggattga tcttctcagt tttatacagt gtcatcattt cgcggttcat ggcttgcgga
     1981 tcgccttgat gacgctcgcg aatcgcggtc atcttcggtg tcacggcttt catcttggcc
     2041 atgctgcggt agctggcggc agacaagggg aagaacatca gcttgatcag cagcgtcagc
     2101 aggatgatgg tccagcccca gttgccgaca aaggcataga tgtacatcat cagccagaag
     2161 ataggtttgg cgatgatggt cagccagcca tagtctttga ccagttccag gccaggcgca
     2221 gtggcttcca gcactttgga ttcctgcgga ccggaataca ggcgcacatc catcgtcacg
     2281 ctggcgcctg gagcgaccgt gcccagcggc aggatattgc cgacagcgta caggttggcg
     2341 gcgacttttt tggcaaagat gtcgcgctgc gctttttcag gcgggatgaa cgccgagacg
     2401 aaataatgct gcaccagcgc gatccagccg ttgtcggatt tggtggcatg ttcctgtttg
     2461 ccttctgcga tcttctcgaa ggccagtttc tggaatttgt cggcatcggt atagaccgca
     2521 gggccggtga aggtactgta gaaataggat tcaccgtccg gcttgttgcc gtcgcgcagc
     2581 agttgcagat acagtgaagg atcgatcgcg tttgcgctgt tgttggtgac ggtatgtttc
     2641 agatcgatga cgtaagcgtc gcgctggaag gtgtaggtct tggttagctt gacgccgttt
     2701 tcctgcgctt ccagcaccag ttggacttca ttgctgccat ccagcacgcg cgcacccggc
     2761 ttggcggtga agaccgattt gtggttgggg aatgggccgc cgatcaggcc ggattccgcc
     2821 agataggtgt ggctggcgct ggcgtcgaac aggaccagat tcctggctgg atcgacggta
     2881 tcgcgatgct tcagcaattc cagacgcttg agttcgccgc ccacggtgtt gacgtcagcc
     2941 ttgacgacgt cagtcatgat ggtgatggtt tcgcttttgg cgcctggagc gactgccaca
     3001 ctggcgtctg tcgctgcggc agttgcctga ggcaccggcg aggtcgatgg atcagcagca
     3061 tcggcggcag gctgcgcagt catcggaaag aacatcgatg gcttgccgtc atggcgttgc
     3121 cagctatccc atagaaacaa cagggagaag gcaaatacaa tccacaggat ggtacgttgg
     3181 atatccataa gagtattgat caatcaatca ggaatagccg caaccgtcgg cggttgcagt
     3241 gggggaggaa gaatccggca cggggtcgaa cccgcccgca tgccagggat ggcacctgca
     3301 cagccgtttg ccagcgagcg cactgccttt ccatgcgccg tgacggcgaa tggcttcggc
     3361 tgcgtaatcc gagcaactgg gatagaaacg gcaattctgc ccgagatagg ggctgatccc
     3421 cagcttgtaa gcgtgcagca gcaggagtaa aggtgttttc attacttatt tcccgacttc
     3481 ccggcaaagg ctggcgcgtt gagcaaaaaa gtgaacaact gcaacagttc cgcgcgcaat
     3541 gccgttttca gttttgcggt gctcgccggc tcgctcctgc tgttgaccgg actgaataaa
     3601 cgcacgatgc agtctacttg gggcaatgcc gcctgccgga acgattcacg gataatgcgt
     3661 ttgatcatgt tccgggtcac cgcgcgcggc gccatgcgtt tggccgtaat gacgcccaga
     3721 cgggctaatg gcaagtcgtt gttgcgggca tagagtatga aatgggcgga ccggtacacc
     3781 ggtcgcaaac gaaaaacgga tgaaaattca tccgttttga cgattcgccg atcgcgtgaa
     3841 tagagatggg aatgatcgtc tgtcacacaa acagcagcgc actgtttcca acgggcatta
     3901 cgccactgcc agacgcttgc gacccttggc gcgacgtgcg ttgattacgg cacggccggc
     3961 gcgggtcgcc atgcgcgcgc ggaaaccgtg ggtgcgcttg cgacgcacga cggaaggttg
     4021 gtaggttcgt ttcatgttgg actcgcttac ttaattttac ttaatcaata atcggcaaaa
     4081 atacatcgga atccctgttt tttctgcgcg tctcatctcg ccctgtcagg cagagaggca
     4141 ttcgagggct gaaaccgtca ggctgcggca cgaacgcaat tcaacggatg gtcaaccttg
     4201 gattaaactg cattttctct ttgtttgtca acgatttata tggttgttcc cctctttcat
     4261 ggataattcg cgccaatgcc agcaggcgca tccggctgcg tctgtcgctt tctactccct
     4321 cacggaacac ggacatcccg cgaaatgcgc aaggatttcc aagatattga cgaaatgatg
     4381 gaataagcga atgcggtaca ttacttctgc gcaaaagtca gaggcgtaca cgatagtaaa
     4441 aa
//
LOCUS       DASRDH010000072         3277 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_879574,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000072 DASRDH010000000
VERSION     DASRDH010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 3277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3277
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_879574"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1508
                     /gene="alaS"
                     /locus_tag="VFA14_01770"
     CDS             <1..1508
                     /gene="alaS"
                     /locus_tag="VFA14_01770"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006464317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HZS80146.1"
                     /translation="HGMKILEAALARNAAGLDGETAFMLYDTYGFPLDLTADICRERN
                     VELDEAGFAIAMERQKSTARAVGRFRMTAGIEYSGGKTRFVGYDVLVQQAKVVALYVD
                     GSAVNEIKAGQDAVVVLDTTPFYAESGGQSGDAGVLAAANALFAVVDTQKIQPEVFGH
                     HGHLAEGSLSVGEAVEAGVDVEQRVRTMRNHSVTHLMHKALREVLGGHVAQKGSLVDA
                     DKTRFDFSHDVPVTADEICRVEEIVNREILANVATEARLMSFDDAVSAGAIALFGEKY
                     GDEVRVLTIGSSRELCGGTHVSRTGDIGLFKIVAEGGVAAGIRRVEAVTGENALTLMQ
                     ALSKRVNEAAAALKTQPEELIQRIAQAQDYIRLLEKELSALKSKLASSQSDEILAQAV
                     SVNGIKVLATTLESADVAVLRETVDSLKGRFKTAVIVLASVKDGKVSLIAGVTADVMS
                     RVKAGELVNFVARQVGGKGGGRPDMAQAGGTDPGGLPKALESVAAWVGSRV"
     gene            1514..2059
                     /locus_tag="VFA14_01775"
     CDS             1514..2059
                     /locus_tag="VFA14_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HZS80147.1"
                     /translation="MCDFRFCPVCATSLVMRADEGEAGKLRLACPDGHWTHWDNPLPV
                     LAGLVEIDGKILLARNVAWAEGRFALITGFMERDETPEQGIAREVKEETNLDADQITL
                     IGVYEFMRRNELIIAYHVRARGEIRLSSELLEYRLVAPPDLRPWPSGTGYAVADWMRR
                     HDLPVQFVEFAGSSIPLVVAG"
     gene            2160..2393
                     /locus_tag="VFA14_01780"
     CDS             2160..2393
                     /locus_tag="VFA14_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008111340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HZS80148.1"
                     /translation="MAIEFHRELDARGLSCPLPILKTKKALADMSSGQILRVTATDTG
                     SVRDFQAFAKQTGNDLLSQFQENQEFIFFMKRK"
     gene            2854..>3277
                     /locus_tag="VFA14_01785"
     CDS             2854..>3277
                     /locus_tag="VFA14_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HZS80149.1"
                     /translation="MNMIEAKKVLETALLCAQEPLSVNDLKRLYVSDDETGSVMTADA
                     IQQLVEELREDWADKGVEIVSLSTGWRFQSRAEMKVYLDRLSPEKPPKYTRATLETLA
                     IIAYRQPVTRGDIEEIRGVTVSSQTVRLLEERNWIEAVG"
BASE COUNT          732 a          788 c         1013 g          744 t
ORIGIN      
        1 aacacggcat gaagatcctc gaagccgcgc tggccaggaa tgctgccgga ctcgacggcg
       61 aaacggcatt catgctgtac gacacctacg gtttcccgct ggatctgacg gccgatatct
      121 gtcgcgaacg caatgtcgag cttgatgagg ccggtttcgc tattgcgatg gagcgtcaaa
      181 aatcgactgc tcgcgccgtc ggcagattca ggatgactgc tggtattgaa tacagcggtg
      241 gcaaaaccag gttcgtcggt tacgacgtac tggtgcagca agccaaggtt gtcgcattgt
      301 atgttgatgg cagcgccgtc aatgagatca aggccggcca ggatgccgtc gtcgtgctgg
      361 acaccacgcc tttctatgcc gagtccggcg gtcagtctgg tgacgccggc gtgctggcag
      421 cggcaaatgc gctgtttgcc gtcgtcgata cgcaaaagat tcaacctgaa gtgttcggcc
      481 atcatggtca cctcgccgaa ggttcgctgt cggtgggtga ggccgtcgaa gccggagtcg
      541 atgtggagca acgtgtgcgc accatgcgta accattccgt tacgcatctg atgcacaaag
      601 ccttgcgcga agtgctgggc ggtcatgtgg cgcaaaaagg atcgctggtc gatgccgata
      661 agacacgttt cgattttagc cacgatgttc cggtgacggc tgatgaaatc tgtcgtgtcg
      721 aggaaatcgt caaccgcgag attctggcca atgtggcgac cgaagccaga ttgatgagct
      781 tcgatgatgc tgtcagcgcc ggtgcgatag ccttgttcgg cgaaaaatac ggcgacgaag
      841 tgcgggtgct gacaatcggt tcgtcgcgtg aattgtgtgg tggcacgcat gtgtcgcgca
      901 ccggcgacat cggtctgttc aagatcgttg ccgaaggcgg cgtggcggct ggcattcgcc
      961 gtgtggaagc ggtcactggc gagaatgcgt tgacgctcat gcaagcgctg agcaagcgcg
     1021 tgaatgaagc cgctgctgcc ttgaagacgc aaccggaaga gttgattcag cgtattgcgc
     1081 aagcgcagga ttacatcaga ttgctggaaa aagaattgtc ggcattgaaa tcgaaactgg
     1141 cgtcgagcca gagtgatgag atactggcgc aggcggtcag cgtcaacggc atcaaggtgc
     1201 tggcgaccac gctggaaagc gccgatgtcg ccgtattgcg cgagaccgtg gacagtctga
     1261 aaggcaggtt caagaccgct gtcatcgtgc tggcttcggt caaggacggt aaggtcagcc
     1321 tgatcgctgg cgttaccgct gacgtgatgt ccagggtcaa ggctggtgaa ctggtcaatt
     1381 tcgtcgcccg gcaagtcggc ggcaagggcg gtgggcgtcc tgatatggcg caagccggcg
     1441 gcaccgatcc tggtggcttg ccgaaggcgc tggaaagcgt tgcggcttgg gtcggcagca
     1501 gggtttgagc ctcgtgtgcg acttcaggtt ctgtcccgtt tgtgcgacat cgctggtgat
     1561 gcgtgctgac gaaggtgagg caggcaagct caggttggct tgtcccgacg gtcattggac
     1621 gcattgggac aatcctttgc cggtgctggc agggttggtc gaaatcgatg gcaaaatcct
     1681 gctggcgcgc aatgtggcat gggcggaagg acgttttgca ttgatcaccg gttttatgga
     1741 acgggacgaa actccagagc agggcatcgc gcgcgaagtc aaggaagaaa ccaatctcga
     1801 tgctgaccag attactttga tcggcgttta tgaattcatg cgcaggaatg aattgatcat
     1861 tgcgtatcac gtcagggcga ggggagagat cagactgtct tctgaattgc tggagtatcg
     1921 tctagtagcg ccaccagatt tgcggccgtg gccgtcagga acgggatatg ccgtggcgga
     1981 ctggatgcgg cgtcatgatt tgccggtaca attcgttgaa tttgctggct cgtctatccc
     2041 gttggttgtc gccgggtaat ttgagtgtgc ggaatgcaac agagtatgtt gcgacgcatc
     2101 aaaataattt atttggggta aaatgctaaa gcgttcatta gaattctcga ttcatcagta
     2161 tggctattga atttcacaga gagctggatg cgcgaggcct gagttgcccg ctgccgatat
     2221 tgaaaaccaa gaaagccttg gcggatatgt cgagcgggca gatattgcgt gtgacggcga
     2281 ccgacactgg ttcggtacgc gattttcagg cgtttgccaa gcaaaccggc aacgatttgc
     2341 tctcgcaatt tcaggaaaat caagaattta tcttcttcat gaagcgcaag tagttaatgt
     2401 acctgcgtga gggcaacgtt cggtcttttg tgcggcgcga ccagcgcaga attgcagcag
     2461 tcggcgccaa tgcccattgc ttggctgtct tcttgtacca tatcttgcaa tactgttggc
     2521 agcctgcaac gaagcggcat tgcgctgagg ctttgtgcta cactgcgtgc agtgttttgt
     2581 tttgcatgtt gcgtcttcgg aaacccagct cggcgccatc tgcaaggtca aaaattgaaa
     2641 tagcgcgaga atcctggacc ccagaattct gtattcgcga ccaggcctta ttttcctgct
     2701 tatccaacct ctataatcga gcgccggaaa acagcaccaa cagcatgcag gcagtagtgc
     2761 tgtgacactg tgacgaaact caatccttgt agctgggctt cgtatcggtc aagccgctag
     2821 aacttttgtt aaatagccgc acaagagtca tacatgaata tgattgaggc caagaaagtc
     2881 ctcgaaacag cattgctgtg tgctcaagag cctttgtcag tgaacgattt gaagaggctt
     2941 tacgtatctg atgatgagac cggaagcgta atgacagcgg acgccatcca gcaactggtg
     3001 gaggaactcc gcgaagactg ggctgacaag ggagtggaaa ttgtcagctt gtcgacaggc
     3061 tggcgtttcc agagccgcgc cgagatgaag gtatacctgg atcgcttgag tccggaaaag
     3121 ccaccgaaat acacgcgcgc aacccttgaa actcttgcca tcattgcgta tcgccagccg
     3181 gtaacgcgcg gcgatattga agaaatccgc ggtgtcactg tcagctcgca gacggtcagg
     3241 ttgctggaag agcgcaactg gatcgaggct gtcggtc
//
LOCUS       DASRDH010000073         3092 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_100758,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000073 DASRDH010000000
VERSION     DASRDH010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 3092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3092
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100758"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            10..492
                     /locus_tag="VFA14_01790"
     CDS             10..492
                     /locus_tag="VFA14_01790"
                     /inference="COORDINATES: protein motif:HMM:NF016873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HZS80150.1"
                     /translation="MIQRRIRQYWRPYHQRLEQELQRMRAEHGVAMLWDAHSIASVVP
                     RFFAGCLPDLNLGTGGGNSCAPELTQQLQAVAVQAAGAGYSHAVNGRFRGGHITRHYG
                     RPEHNIHAVQLEQTQLTYMEEQAPFAFDERRAARARPILRQFMQVMLDWARTHGRAPH
                     "
     gene            complement(504..2031)
                     /locus_tag="VFA14_01795"
                     /pseudo
     CDS             complement(504..2031)
                     /locus_tag="VFA14_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011979390.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
     gene            complement(2047..2289)
                     /locus_tag="VFA14_01800"
     CDS             complement(2047..2289)
                     /locus_tag="VFA14_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016836063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HZS80151.1"
                     /translation="MDKPQFFEDLQSKISKAIESLPAKDIEKNVKAMLSQGFSRLDLV
                     TREEFDIQAQVLAKTRARLEELEARVRELEAYQKAS"
     gene            complement(2412..2945)
                     /locus_tag="VFA14_01805"
     CDS             complement(2412..2945)
                     /locus_tag="VFA14_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007877907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HZS80152.1"
                     /translation="MTHEQAKNGHLHAFQAELLDRIESAQAGPDKHNSRLGVVVGGHH
                     CLIDLREVSGIIDLMPIINRVPLTRDWYLGLANVDGNLIGVVDLARFCGGEQQVRDKD
                     SRVIVTSSVVPVACGFLVSRALGLRHTQEMALQENAGGVAMRIAARPYLDRSRQEWAE
                     LSLTAIVDDTRFLQVGL"
BASE COUNT          604 a          935 c          958 g          595 t
ORIGIN      
        1 gacgagacca tgatccagcg ccgcatccgc caatactggc gaccgtatca tcaacggctg
       61 gagcaggagt tgcagcgcat gcgcgccgaa catggggtgg cgatgttatg ggatgcgcat
      121 tccatcgcct ctgtggtgcc acgttttttt gcaggctgtc tgccggattt gaatctgggc
      181 accggcggcg gcaattcctg cgcaccggaa ctgacgcaac aattgcaggc agttgctgtc
      241 caggcggctg gtgccggcta cagtcacgcg gtcaacggcc gcttcagggg cggtcatatc
      301 acgcgccact acggcaggcc ggaacacaac atccatgccg tacaactgga acagacgcag
      361 cttacctata tggaagaaca agcgccgttt gctttcgacg aacgacgggc ggcgcgggcg
      421 cgaccgattc tgcggcaatt catgcaagtg atgctggact gggcgcggac gcatggtcgt
      481 gcgccgcatt gatcgatgcg cagtcactgc tcgcgcaagg cgcggcgata gatattgaat
      541 cgcttcggcg atgtgcgcct tgccgatgac tggctcttgc gccagatcgg cgatggtgcg
      601 ggcgactttc agcacgcggt gacaggcgcg cgccgaccag ttcagccggg tcatggcggc
      661 gcgcagcaac gcttcgccag cggcgtcggg tttgcagtgg aggtcgattt cgccggtcga
      721 cagtaaatga ttgccctttt gctgacgttg caattgcagc ccgaagctgc gcgccacccg
      781 ttcggcgatg cgggaggagg tttcgccatc ggcctgttgc agcaattccg cgtgcgcgag
      841 cgcgccgacc tggatctgca tatcgatgcg atcgagcagc ggaccggaga tttttccctg
      901 gtagcgcgcg acctgatccg gcgtgcagcg gcatattctt tcagcatggc cgagaaagcc
      961 gcatgggcag ggattcatgg ccgcgatcaa ctgaaagcgt gccgggaaat cggcttgccg
     1021 cgccgcacgc gagatggtaa tgcggcctga ctccagcggt tcacgcaata cttccagcac
     1081 tttgcgatcg aattccggca actcatcgag gaaaagtaca ccacgatgcg ccagcgaaat
     1141 ctcgcccgga cgcggaacgc cgccacctcc caccagcgct gcgccggagg cggtatggtg
     1201 cggtgaacga tagggacgtg tcttccagcg cgttgcggaa aagccgccgg tcagcgactg
     1261 taccgctgcc gactccaagg cttcttcatc ggtcatcggc ggcaggatgc cgggaaaacg
     1321 tgccgccagc atggacttgc cggtgccggg cggccctacc agcagcacgc tgtgtccgcc
     1381 ggctgcggca acttccaggg cgcgcttggc ttgcagttgc cctttgacct cggcgaagtc
     1441 gaggtaggcc gacacgctgg cgggcacgaa cggccgatga cgcgtcagtc tggcagcgac
     1501 atcctgtccg gcgaaatgcg cgcagacttg cagcaaggaa tcggcaggga aaatggcggc
     1561 ggcgctgacc agcgccgctt catcggcgtt ctcgcgcggc aggatgaaac cgcgccggca
     1621 gccatcgctg ccctgcggca tgtgcatggc atacgtcatc gccagcgcgc cgcgaatcgg
     1681 acgcagatcg ccggacagag aaagctcgcc ggcgaattcg tagccgtcta gcgcatcccc
     1741 cggcatctgg ccggaggcgg cgaggatgcc caaggcgatc ggcaggtcga aacggcccga
     1801 ctcctttggc agatctgccg gagcgagatt gacggtgatg cgtttcgccg ggaattcgaa
     1861 gcgcgcgttt tgcaaagccg cacgcacgcg ctccctggat tccttgactt cggtttccgg
     1921 gagaccgacg atggtgaaag aaggcaggcc gttggccaga tgggtttcca ccgtgacctg
     1981 tggcgcatgc atgcccgtca gcgcgcggct tctcaataca gccagactca tcgcaaaggc
     2041 atgcgatcaa gatgctttct gatatgcttc gagttcccgc acccgggctt cgagttcttc
     2101 cagtctggcg cgggtcttgg ccagcacctg cgcctgaatg tcgaactctt cgcgcgtgac
     2161 cagatcgagc ctggaaaagc cctggctcag catcgccttg acgttttttt cgatatcttt
     2221 ggctggtaaa ctctcgatgg ctttgctgat tttcgattgc agatcctcaa agaattgcgg
     2281 tttgtccatg gcgcttcctt tatcggtaat aactgtccgg caagtgtagc gcgttcatcg
     2341 tactgacgga aaagaataac tttgccgatg cggaagaacc gaagaaaggc tgcaacgacg
     2401 cgaggcagga actacagccc gacctgcagg aagcgtgtgt catcgacgat cgctgtgaga
     2461 ctgagctcgg cccactcctg ccggctgcga tcgagataag gtcttgccgc aatccgcatg
     2521 gcgacgccac cggcattttc ctgcagggcc atttcctggg tatgtctcaa acccagcgcg
     2581 cgcgatacca gaaaaccgca cgcgaccggc acgactgagg aagtgacgat gacacgacta
     2641 tccttgtcac gcacctgttg ctcaccgccg cagaaacgcg caaggtcgac caccccgatc
     2701 aggttgccgt ctacatttgc cagccccaga taccagtccc tcgtcagcgg caccctgttg
     2761 atgatcggca tgagatcgat gatgccgctt acctcgcgca gatcgatcag gcagtggtga
     2821 ccgccgacga ccacgcccag ccggctgtta tgtttgtccg gaccggcttg cgcagactct
     2881 atacgatcga gtagctccgc ctggaatgca tgcagatggc cgttcttcgc ttgctcatgc
     2941 gtcactcggt cattcctgcc gggtctgcgt cagcagactg gcggcccgat gaggatttgc
     3001 agcgtaacag agcgcctgaa aaggcgactg gacggccccg aagaaaatcg tcagcacgtt
     3061 ctcagagcgc gatcatctcg aagtcttcct tg
//
LOCUS       DASRDH010000074         3040 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_879391,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000074 DASRDH010000000
VERSION     DASRDH010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 3040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3040
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_879391"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1261)
                     /gene="hisS"
                     /locus_tag="VFA14_01810"
     CDS             complement(<1..1261)
                     /gene="hisS"
                     /locus_tag="VFA14_01810"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HZS80153.1"
                     /translation="MSENRKIEKIVGVKGMNDILPADAPLWELFENTVQSVLKSYGFQ
                     QIRTPVVEPTALFAHGLGEVTDIVEKEMYSFTDAMNGDKLSLRPENTAGVVRAALEHN
                     LTYDGPRRLWYAGPMFRHERPQRGRYRQFHQVGAEALGFTGPDIDAELIMMCQRLWDD
                     LGLQDIRLELNSIGDAEERNRHRADLIAYFEQHEDLLDTDARRRLHANPLRILDTKNP
                     AMQDMVNAAPRLLDYLDAASRAHFEGVQKILHHNNIPFTINPRLVRGMDYYNRTVFEW
                     VTDQLGAQGTVCGGGRYDPLIEMFGGKPTPACGFAIGVERLLELMKASGEQYAPNQCD
                     VYLVHQGEAAQLQSFVLAERLRGAGLDVVLHCASANAGGSFKSQMKRADASGAAFAVI
                     IGEDEVANATATVKNLRAPEGKSSQQVT"
     gene            complement(1333..2601)
                     /gene="ispG"
                     /locus_tag="VFA14_01815"
                     /gene_synonym="gcpE"
     CDS             complement(1333..2601)
                     /gene="ispG"
                     /locus_tag="VFA14_01815"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007877277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HZS80154.1"
                     /translation="MPSSPAPIASGPLMRRQSRCAIIRHGDRQVAVGGGAPVMVQSMT
                     NTDTTDAINTAIQVRDLAQAGSEVVRITVNTPAAAAAVPAIREQLDRMGVDVPLVGDF
                     HYNGHTLLDDYPDCARALSKYRINPGNVGKGARRDTQFAQMIAAARRHDKPVRIGVNW
                     GSLDQALLARIMDKNAQRREPWSAQAVMHEALVTSTIENAQRAEQLGLDGNKIILSCK
                     VSGVQDLIAVYRELARRCDYPLHLGLTEAGMGSKGIVASTAALSVLLQEGIGDTIRIS
                     LTPEPSGDRTREVIVGQEILQTMGLRKFTPMVIACPGCGRTTSTVFQDLANKIQTYLR
                     NQMPVWKGKYPGVESMKVAVMGCIVNGPGESRHANIGISLPGTGELPAAPVFIDGEKR
                     MILRGDRIAEEFQSVVLDYVQTHYRKQTAA"
     gene            complement(2624..>3040)
                     /locus_tag="VFA14_01820"
     CDS             complement(2624..>3040)
                     /locus_tag="VFA14_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS80155.1"
                     /translation="AYAKLLGLDANVLVALLPQQGAVHHTSVMPQRMLSIPFSESSLP
                     LQNHSRLLPASIAGGIAMAVLAGAAFVIDRTDWFNEAPQLAWLKSSAAGSMIAAVSEH
                     VVDVVTLSPSPTKAAMSETGLADGRQRGTPEPEINH"
BASE COUNT          630 a          903 c          830 g          677 t
ORIGIN      
        1 gcgtgacctg ctggctgctt ttgccttccg gcgcgcgcaa gttcttcacc gttgccgtcg
       61 cattggcgac ttcatcttct ccgatgatga ccgcaaacgc tgcaccgctg gcatccgctc
      121 tcttcatttg cgatttgaag ctgccaccag cattcgccga tgcgcaatgt agcacaacat
      181 ccaacccggc accgcgcaat ctttccgcca atacgaagga ttgcaattgc gcagcctcgc
      241 cctgatgtac cagatacaca tcgcattgat tcggcgcata ttgctcgccg ctggccttca
      301 tcaattcgag caaacgctcg acgcctatag caaaaccaca ggctggcgtt ggtttgccgc
      361 cgaacatttc gatcaatgga tcgtaacggc cgccgccaca taccgtaccc tgagcgccga
      421 gctgatcggt gacccactcg aacacggtgc gattgtaata gtccatgcca cgcaccagac
      481 gcggattgat ggtgaacgga atattgttgt gatgcaatat tttttgcacg ccttcaaaat
      541 gcgcgcgcga tgctgcgtcc aggtaatcca gcaacctggg cgcagcattg accatgtctt
      601 gcatggctgg atttttggta tcgagaatgc gcagaggatt ggcatgcagg cgacgtcggg
      661 catccgtgtc gagcaagtcc tcgtgttgct cgaagtaagc aatcagatcg gcacgatgcc
      721 tgttgcgctc ttcggcatcg ccgatggaat tgagttccag gcggatgtcc tgcagcccca
      781 agtcgtccca caggcgctgg cacatcatga tcagttcggc atcgatgtcc ggaccggtaa
      841 agcccagcgc ttccgcgccg acctggtgga actggcggta gcggccacgc tgcggacgct
      901 cgtgacggaa catcggacca gcgtaccaaa gacgcctggg gccatcataa gtcagattat
      961 gttcgagcgc cgcgcgcacc acgccggcgg tattttccgg acgcaggctc agcttgtcac
     1021 cgttcatcgc atcggtaaag gagtacatct ccttctcgac gatgtcggtg acttcgccca
     1081 ggccatgcgc aaacaaggca gtcggctcga cgacaggcgt gcggatttgc tggaaaccgt
     1141 agctcttcag caccgattgc acggtgttct cgaagagttc ccacaatggc gcatcggcgg
     1201 ggagaatgtc gttcattcct ttgacgccga cgattttttc gattttcctg ttttcagaca
     1261 tgtttgtaat caataagctt cgttcacctg ggaacgcccg gcctgcactg gaccctgccg
     1321 caaatgcagc gctcaggctg ccgtttgttt gcggtaatgc gtttgcacat aatccagcac
     1381 gacggactgg aattcttcgg cgattcggtc gccgcgcaag atcatccgtt tttcgccatc
     1441 gatgaagacc ggcgccgcag gcaattctcc ggtgcccggt agactgatgc cgatattggc
     1501 gtgccttgac tcgccggggc cgttgacgat gcagcccatg acagcgactt tcatgctttc
     1561 tactccggga tatttaccct tccataccgg catctggttg cgcagatagg tttgaatctt
     1621 gttcgccaga tcctgaaaga cggtcgaggt agtccggcca cagcccggac aggcgatcac
     1681 cattggcgta aacttacgca agcccatagt ttgcaggatt tcctgtccaa cgatcacttc
     1741 gcgggtacgg tcaccgcttg gttctggtgt caacgagatg cgaatggtgt ccccaatacc
     1801 ttcctgcagc agcactgaca atgccgccgt cgaagcgaca atgcctttgc tgcccatacc
     1861 ggcctctgtc aatcccagat gcaggggata atcacagcgg cgcgccagtt ctcgatagac
     1921 ggcaatcaga tcttgcacgc cggagacttt gcacgacagg atgattttgt tgccatccaa
     1981 gcccagttgt tcggcgcgct gcgcattctc gatagtggat gtcaccagcg cttcatgcat
     2041 caccgcctgc gctgaccaag gctctcgacg ctgcgcgttt ttatccatga tacgcgccag
     2101 cagggcttga tccagactac cccagttgac gccaatgcgt accggtttat catggcggcg
     2161 agcagcggcg atcatttgcg caaactgtgt gtcacgtctg gcgcctttgc cgacattgcc
     2221 cggattgata cgatattttg ataaagccct ggcgcaatca gggtagtcat caagcaaagt
     2281 atgaccgtta tagtggaagt caccgactag cggtacatcg acccccatgc gatcgagctg
     2341 ctcacgaatg gccggcaccg ctgctgctgc tgccggcgtg ttgaccgtga tgcgtaccac
     2401 ttctgaaccg gcctgcgcca gatcccggac ctgtatcgcg gtgttgatgg cgtcggtggt
     2461 atcggtgttc gtcatcgact gcaccatgac cggcgcgcca ccgcccactg caacctgacg
     2521 gtcaccatga cggatgatgg cgcaacggct ttgacggcgc atcagcggac cagatgcaat
     2581 cggagcaggt gatgaaggca tagatgcgtt atttcagttc gcttcagtgg tttatctcag
     2641 gctcaggcgt gccacgctgc cgcccgtctg ccaatccagt ttcggacatt gctgctttcg
     2701 tcggagacgg cgacagcgtc acgacatcga cgacatgctc cgataccgca gcaatcatgg
     2761 aaccggccgc gctggacttc agccatgcca gttgtggcgc ctcattgaac caatccgtcc
     2821 gatcgatgac gaaagctgcg ccggccaaca ccgccatggc aataccgcca gcgatcgatg
     2881 ccggcaacag cctgctgtga ttctgaagcg gcagcgacga ttccgaaaaa ggtattgaca
     2941 gcatacgttg cggcatgact gatgtatgat gcacggcccc ctgctgaggc agtaaagcaa
     3001 ccaggacatt ggcatccaga cccagtaatt tggcatacgc
//
LOCUS       DASRDH010000075         8402 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1185771,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000075 DASRDH010000000
VERSION     DASRDH010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 8402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8402
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1185771"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..1007)
                     /locus_tag="VFA14_01825"
     CDS             complement(87..1007)
                     /locus_tag="VFA14_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010119622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZS80156.1"
                     /translation="MLGTLSDLDLRLIRVFLAVVDAGGVSVAQTTLNVGQPTISTQLS
                     TLETRLGFRLCDRGRSGFLLTAKGERFYERSKKLLGAVEEFSAEARNMDKKLVGTLNI
                     GLIGHTPVSQNARISEAIASFRLRDEAVRFFISVRSPGDLEEHLLSGQIQIAVGYFWH
                     RVPILEYTPLFVEQQIAYCGRGHPLFKRAERLDSSEVADYEWTWRTYPLPEVQLSTTP
                     SKVTAQADNMEAVALLILSGHHLGYLPQHFAAPYVKQGLLAALNPEKLSYEVTFHMVI
                     RQHSRQSQSPIVQAFLEDLKKAHLSGAVDV"
     gene            1158..2111
                     /gene="speB"
                     /locus_tag="VFA14_01830"
     CDS             1158..2111
                     /gene="speB"
                     /locus_tag="VFA14_01830"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012733264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HZS80157.1"
                     /translation="MSDNYFQPQSGNEMPRFGGIATMMRLPHAKDAARLDACFVGVPF
                     DLGTSNRAGARFGPRQIRTESVLLRPYNMATRAAPFDSLQVADVGDVATNPYNMVDSI
                     ARIESAYDDILAHGCRPISLGGDHTIALPILRAMHRKYGHVGLIHVDAHADVNDTMFG
                     EKIAHGTLFRRAVEEGLLDCDRVVQIGLRGTGYAAEDFDWCRAQGFRVVPAEECWNRS
                     LAPFMKEVRAGVDGGPVYLSFDIDGIDPAYAPGTGTPEIAGLTVPQALEIIRGSWGLN
                     IIGADLVEVAPPYDPLGTTALLGANLAYEMLCVLPGVKRRD"
     gene            2192..3682
                     /locus_tag="VFA14_01835"
     CDS             2192..3682
                     /locus_tag="VFA14_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012217543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HZS80158.1"
                     /translation="MNAQENVDLVGSNTGLEIESRSIDYIPENERHAKLRSQGPFWFL
                     GNFHFLTISIGFVGPSMGLSAVWTTLAGALGIMFGTIFMAFHGSQGPELGLPQMIQSR
                     AQFGYRGVTLALMATLFVFIGFNVVNISLIMDGLKNVFGLDPTIVGITAVVAGGLLAI
                     YGHDLMHKAFKWALIATLPFYALVTGTLVFGTKGTVAPLTTFGFNWVAFATQFAIGAS
                     YNISYAPYVSDYSRYLPKNTNRAKLITTIFLGASLSGTWMIGLGAWLAQQLKASDALV
                     ALNHVGSAMFPGFGSAIVIVSVAGFLPVIALNTYSAMLTLLTGVDSLHKIKATRCARV
                     IAALAISGILLACILSIRGNGVTLLHTFLVLLLYFLVPWTAVNLVDYFFVRKGRYAIP
                     HFFTPKGIYGAWQVRGIAAYLIGFVAMTPFFYIVDATMNQEIFVGPMARLMSGVDIAW
                     LVGLAVAGTTYYALSRSLHLRAEETVIRSFKESDFVLMAHHAVGDE"
     gene            3682..3893
                     /locus_tag="VFA14_01840"
                     /pseudo
     CDS             3682..3893
                     /locus_tag="VFA14_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009239173.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(3880..5172)
                     /gene="gabT"
                     /locus_tag="VFA14_01845"
     CDS             complement(3880..5172)
                     /gene="gabT"
                     /locus_tag="VFA14_01845"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015603826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HZS80159.1"
                     /translation="MINKDLQSRKNAATPRGVGVMCDFYAARAENAELWDIEGRRFID
                     FASGIAVNNVGHRHPKIVAAIRNQLDEGFIHTAYQIVPYASYVELAEKINRLAPGSRP
                     KKTVFFTTGAEAVENAIKIARAATGRSGVIAFNGGFHGRTMMSMALTGKVVPYKIGFG
                     PFPAEIYHAPYPCELHGISVEDALDGIAQRFRADIDPQCVAAFIFEPVQGEGGFYMAP
                     PEFVLGLRALCDQHGILLIADEIQTGFARTGKLFAMQYYDVEADLMTIAKSLAGGMPL
                     SGVVGRADLMDAVQPGSLGGTYAGNPLAIAAAHAVLDVIAEENLLVRAISLGVQLKER
                     LASIKARLPQLADVRGPGSMIAAEFFDRKTEAPDPVFTKQVQTRALERGLILLICGVH
                     GNVIRFLFPLTIQQTVFDEGLAILEDVLTVDAAVGHFE"
     gene            5388..6152
                     /locus_tag="VFA14_01850"
     CDS             5388..6152
                     /locus_tag="VFA14_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HZS80160.1"
                     /translation="MMMRHSLLCTLLLTSAIAALPATAADPAAHPVIKREVDLPPSAT
                     LHYRIKAKQSGLQVNGEALVKWNNSDKKYVAATETRAMLLGKILETSSEGDIDSYGLA
                     PARFVDKRFRREASITTFDRELHTINFTQSTEHYPLKGGEQDRTSIIWQLIAIARANG
                     DKFTPGSEWLFFVAGPRDAEQWSFQVTGHEKIRLRQGEIDAIHVLRAQPSDDQGQKLD
                     IWIAPSLDWYPARLRFTDPDGDFIEQTLDEINKNPG"
     gene            6189..6923
                     /gene="queC"
                     /locus_tag="VFA14_01855"
     CDS             6189..6923
                     /gene="queC"
                     /locus_tag="VFA14_01855"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007879536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HZS80161.1"
                     /translation="MSTPCLSVPHRALVLFSGGQDSATCLAWALSRYDRVETIGFDYG
                     QRHAIELTVRPALLEKIRGFSSEWHDRLSADHMVDLSLIGKISDTALTRDVAISMQEN
                     GLPNTFVPGRNLLFMTVAATMAYRRGLDVLVGGMCETDFSGYPDCRDDTMKALQVTLN
                     LGMATQLKVETPLMWIDKAATWRLAEDLGGQALVDLIRSDTHTCYLGERGALHDWGHG
                     CGICPACDLRARGYQRYIMGKTGNHA"
     gene            complement(6936..>8402)
                     /locus_tag="VFA14_01860"
     CDS             complement(6936..>8402)
                     /locus_tag="VFA14_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006461494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HZS80162.1"
                     /translation="FKDDAYTRGLNVFTTITKTDQDAAYLALRRGIINYERRHGYRGP
                     EGYMEIPTSSKEAADDAIESELADHPDSDDLVAAVVLKATNKEITAVLATGDEIRISG
                     SGLGFAGNLLSEKAPPQKRVRRGAIIRVFQDGKDWIVTQMPEVESAFVALNPNDGAIR
                     ALVGGFDFNRNKFNHVIQAWRQPGSSFKPFIYSSSLEKGLSPATIINDAPLTFTQTGG
                     QVWQPKNYGGKFEGPISMRRALQKSINMVSIRILERVGVKYAQEYITRFGFDADKNPP
                     YLTLALGAGAVTPLQMVVAYSVFANGGYKVNPYLISKITDSNGKVLSEARPARAGDES
                     NRVIDPRNAFVMDSMLRDVVRHGTAVKVLSLNRTDLAGKTGTTNDSMDAWFAGYQPNL
                     AGIAWMGFDQPKSLGDRETGGGLALPIWINYMQKVLKSAPMVDRTVPSGLLESNGEYY
                     YAEYPPNASVHDLGLGSQNVAVLPEEEKNRNQIKNELF"
BASE COUNT         1792 a         2406 c         2311 g         1893 t
ORIGIN      
        1 aaatagtgac tgctgacttt ttgagttggt gttacctttt ttgcggatgt caagaagaga
       61 agaacggaat gttgtggtct ctcttgttat acatcgacgg cgccagagag gtgtgctttc
      121 tttaaatctt ccaggaaggc ctgaacaatc ggactttgac tttgccggct gtgctgccgg
      181 atgaccatgt gaaatgtcac ttcgtaactg agtttttcgg ggttaagcgc cgcaagcagc
      241 ccctgtttca cgtagggggc tgcgaaatgc tgcggaagat atccgaggtg atggccagat
      301 agaatcaaca gtgctaccgc ttccatattg tcggcctgcg cggtcacctt actcggcgta
      361 gtcgaaagtt gcacttcggg tagaggatac gtgcgccacg tccactcgta gtctgctacc
      421 tccgatgaat caagccgctc ggctcgtttg aaaagcgggt ggcctcgccc gcaatatgca
      481 atctgctgtt cgacgaagag gggcgtgtat tcgagtatcg gcacccggtg ccagaaatag
      541 ccgactgcga tctggatttg cccgctcaaa agatgttctt ccaggtctcc gggtgacctg
      601 accgaaataa agaaacgaac agcttcgtcg cgtagcctga aagaggcgat tgcctcacta
      661 atgcgggcat tctggctaac gggcgtgtgc ccgatgagac caatgttcaa ggtgccgaca
      721 agcttcttgt ccatgttccg ggcttcggca ctaaactcct caacagcacc gagcagcttc
      781 ttgctgcgct cgtaaaagcg ttcccccttg gcagtgagca gaaagccgct tcggccacga
      841 tcacataatc gaaagccgag ccgggtctcg agcgtcgata actgcgtact aatggtcggc
      901 tgaccgacgt taagcgttgt ttgcgcgaca cttactcctc cagcgtcaac aacggcgagg
      961 aagacgcgga tcaaccgcag atcaagatca gacagggttc cgagcacgat tgctccttgg
     1021 cgagcattga tacatattga tacatcaatg tcaacgtcgc gaattcgtaa ttcatttcta
     1081 atttttgacc acctacaact acacacatac ggccagcctg aacgatacgg cagaacgaat
     1141 gaaagtggag tgagattatg tcagataatt attttcagcc ccagagcggg aacgaaatgc
     1201 cccggtttgg cggcatcgcc acaatgatgc ggctacctca tgcgaaggac gcggccaggt
     1261 tggacgcctg ttttgtcggc gtgccatttg accttggcac ctcgaaccgg gctggtgcaa
     1321 ggttcggccc gcggcagatc cgcacggagt cggtgctgct tcggccatac aacatggcga
     1381 cacgtgccgc gccgtttgat tcgttgcaag tcgccgatgt cggcgacgtc gcaacgaatc
     1441 cctacaacat ggttgattcg attgcacgga tcgaatccgc ctacgacgac attctcgcgc
     1501 atggctgccg gcccatttcc ctcggcggcg atcacaccat cgcgttgccg atcctgcgcg
     1561 cgatgcatcg caagtatgga cacgtgggcc tcattcacgt ggacgcacat gctgacgtca
     1621 acgacacgat gtttggcgaa aaaatcgcgc atggcacgct gttccgacgg gctgttgaag
     1681 aaggtttgct cgactgcgac cgcgtcgtac agatcggttt gcgcggcacc ggttatgcgg
     1741 cggaagactt cgactggtgt cgtgcccaag gcttcagggt tgtgccagca gaggagtgct
     1801 ggaatcgttc actagcccca ttcatgaaag aagtgcgagc cggcgtggac ggtggcccgg
     1861 tttacctgag cttcgacatc gacgggattg accctgcgta cgcgcccggg accggcacgc
     1921 cggaaattgc cggactcacc gtgccgcaag ctctagagat tattcgcgga tcatggggct
     1981 tgaacatcat tggcgccgac ctcgttgagg ttgcgccccc ctatgaccct ctgggcacca
     2041 cagcgttgct gggcgccaat ctcgcctacg agatgctctg cgtcctcccg ggcgtcaaac
     2101 gccgcgactg actcaggcag cctggccatg cacattttga cgcaagtacc ggcgcgactc
     2161 ttcacgtttc aaatttggag actcattcac catgaatgca caagagaacg tcgatcttgt
     2221 cggctcgaac acgggcctag aaatcgaaag tcgatcaatc gactacatcc cggaaaacga
     2281 acgtcacgcc aaactgcgca gtcagggacc attctggttc ctggggaact ttcactttct
     2341 cacgatttcg attggctttg ttggcccgag catgggtttg tcggccgtct ggacgacatt
     2401 ggctggcgct ctcggaatca tgttcggcac gatcttcatg gctttccacg gctcgcaggg
     2461 accggagcta gggctaccgc agatgattca gtctcgggcg cagttcggct atcgcggggt
     2521 tacattggcg ttgatggcca cgctattcgt cttcattggc tttaacgtgg taaacatctc
     2581 gctcatcatg gacggcctga agaacgtgtt tgggctggat ccgacgatag tcggcatcac
     2641 tgcggtggtc gcgggcggtt tgctggcgat ctacgggcac gacctgatgc ataaggcgtt
     2701 caagtgggcg ttgatcgcga ccttaccctt ttacgcgctt gtcacaggca ccctggtgtt
     2761 tggtacaaaa ggtacggtcg caccgctgac gaccttcggg ttcaactggg tcgcgtttgc
     2821 aacccagttt gcgatcggcg ccagctacaa catttcgtac gcgccgtacg tgtctgacta
     2881 ttcgcgctat ctgccgaaga acaccaaccg cgccaagctg atcacgacaa tctttctggg
     2941 tgcgtctctt tccggcacat ggatgatcgg tctgggcgcg tggctggctc aacaactgaa
     3001 ggcgtcagac gcgctggtgg ctttgaatca cgtgggctcg gcgatgtttc ctggattcgg
     3061 cagcgccatt gtgatcgtgt ctgtcgcggg cttcctcccg gtcattgcgc tgaataccta
     3121 cagcgcgatg ttgacgcttc tcaccggcgt ggattcgctc cacaagatca aggcgacgcg
     3181 gtgcgcccgc gtcatcgcgg cgctggcgat cagcggcatt ctcctggcct gtatattatc
     3241 aatcaggggt aatggcgtca cgctgctgca cacgttcctg gtacttcttc tgtacttcct
     3301 ggtgccttgg accgccgtca acttggtcga ctacttcttc gtgcgcaagg gtcgctacgc
     3361 aatccctcac ttcttcaccc cgaaaggcat ctacggggca tggcaggtac gcggtatcgc
     3421 ggcctacctg attggcttcg tcgcgatgac tccctttttc tacatcgttg acgcaacgat
     3481 gaatcaggag attttcgtgg ggcctatggc acgtttgatg agtggcgttg atattgcatg
     3541 gctcgttggt cttgctgtag cgggtaccac gtattacgcg ctgagccgtt cgctacatct
     3601 gcgcgccgag gagacagtga tcaggagttt caaggaaagt gattttgtat tgatggcaca
     3661 ccacgctgtg ggggacgagt gatgaaatgt gaggtgatca agctgcagga ggtgaaaaag
     3721 agatttatcc tgttgccacg ccgccgggtg gtggagcaca gcttcggccg actcaagcgt
     3781 ttccgcggac tggctcgcga ctatgagcgt ttgcccgaaa ccctctcagc catgcattcc
     3841 gtcatcttca ccatgttgat gcggttcatg ccattccgat tattcaaagt gcccaaccgc
     3901 tgcgtcaacc gtcagcacat cttccaaaat cgcgaggcct tcgtcgaata cagtttgttg
     3961 aatcgtcagc gggaacagga agcggataac attgccgtgt acgccacaaa tcagcaggat
     4021 caacccgcgt tcgagcgcgc gcgtctgcac ttgcttggta aacaccgggt caggtgcttc
     4081 ggtcttccgg tcgaagaact cggccgcgat catgctaccc ggaccgcgca cgtcggcgag
     4141 ttgcggcaac cgtgccttga tgctggcaag tcgctctttc agttgcacgc caaggctgat
     4201 cgcccgcacc aaaagatttt cttcggcgat tacgtcgaga accgcgtgtg ctgctgcaat
     4261 tgcgagcggg ttacccgcat aggtgccgcc gagactgccc ggttgcacag catccatcag
     4321 gtcagcgcgg ccgaccacgc ctgacagtgg catgccaccc gcaagactct tcgcgatcgt
     4381 catcaagtca gcttcgacgt catagtactg catcgcgaac agcttgcccg tacgcgcgaa
     4441 gcctgtctgg atttcgtcgg cgatcagcag aatgccgtgc tggtcgcaaa gggcacgcag
     4501 tccgagtacg aattccggtg gtgccatgta gaaccctccc tcgccttgca ccggttcgaa
     4561 gatgaaggcg gcaacacact gcggatcgat gtcagctcta aaacgctgcg caataccgtc
     4621 caacgcatct tcaacgctga tgccgtgcaa ttcacacgga tatggcgcgt gatagatctc
     4681 tgccgggaac ggcccaaagc cgatcttgta cggaacgacc ttgccagtca gcgccatact
     4741 catcattgtg cggccgtgaa acccgccgtt gaaggcgata acacctgaac gtccggttgc
     4801 tgcgcgggcg atcttgatcg cgttttcaac ggcttcggca cccgtcgtaa agaacacagt
     4861 tttcttcggc cgtgagccgg gtgcaaggcg attgatcttc tctgcgagtt cgacgtatga
     4921 tgcatatggg acgatctggt acgcggtatg gatgaagccc tcatcgagct gattgcgaat
     4981 tgccgcgaca atcttcggat ggcggtgacc tacattgttg acagcgatgc cgcttgcaaa
     5041 gtcgatgaag cggcgccctt cgatatccca aagttccgcg ttttcagcgc gtgcagcgta
     5101 gaagtcgcac atcacgccga cgccgcgcgg cgtagcggca ttcttacggg actgcaggtc
     5161 tttgttgatc atgactgttc caaattggtt gagtgcatgg gtagttgtat gtggtcaaaa
     5221 gtcaaaaata aattaccaat tcgcaacgtt aacattggca gattgatatg tatcaatact
     5281 taatgcgctt ttgcagcaaa atctttcgca gccttgatcg atgctcgtcc ctgagcagta
     5341 agcgggccaa acaggttctg tcatattcct cacccttgat tctctccatg atgatgcgtc
     5401 actcactgct ttgtacctta ctcctgacga gcgccatcgc cgccttgcct gccaccgccg
     5461 ccgatccggc cgcgcatcca gtcatcaaac gcgaagtcga tctaccaccg tcggcaacac
     5521 tccactaccg catcaaagcc aagcaaagcg gcctgcaagt caatggcgaa gccctggtca
     5581 aatggaataa ttccgacaaa aaatacgtgg ccgccaccga aacccgcgcc atgctgctcg
     5641 gcaagattct ggaaaccagc agcgaaggcg acatcgacag ctatggtctg gcgccagcgc
     5701 gcttcgtgga taaacgattc cgccgcgaag catcgatcac gaccttcgat cgggaactcc
     5761 ataccatcaa cttcacgcag tcgaccgaac actatccact caaaggcggt gaacaggacc
     5821 gtaccagtat catttggcag ctgatcgcca tcgcacgcgc caatggcgac aaattcactc
     5881 ccggttcgga atggctgttc ttcgttgccg gtccgcgcga tgccgaacaa tggagctttc
     5941 aggtcaccgg ccacgaaaaa atcaggctca gacaaggcga aatcgacgca atccatgtgc
     6001 tgcgcgcaca gccatcggat gatcagggcc agaaactcga tatctggatc gcaccgtcat
     6061 tagactggta tcccgcgcgc ttgcgcttca ccgacccgga tggcgacttc atcgaacaga
     6121 cactggacga gatcaacaaa aaccccggtt gatcacgatt gacagtgtgt atgccgcgaa
     6181 acacaaatat gagcactccc tgtctctccg tcccacatcg cgcgctggta ttgttcagtg
     6241 gcggccagga ttccgccacc tgcctggcct gggccttgtc gcgctacgac agggtagaaa
     6301 ccataggttt cgactatggt cagcgtcacg ccatcgaact gacagtacgg ccggcgctgc
     6361 tggaaaaaat acgcggcttt tcgagcgagt ggcatgacag gctcagtgca gaccacatgg
     6421 tcgatctgtc tctgatcggc aaaatttccg acacggcctt aacgcgcgat gtcgctattt
     6481 ccatgcagga aaacggcttg cccaacacct tcgtaccggg ccgcaatcta ctgttcatga
     6541 ccgttgcggc gaccatggcg tatcggcgcg gcctcgatgt gctggtcggc ggcatgtgcg
     6601 aaacggattt ttccggctac ccggattgcc gcgatgacac catgaaggcg ctgcaggtca
     6661 cgctcaatct cggcatggcc acgcaactga aagtagaaac accactgatg tggatcgaca
     6721 aagcggcaac ctggcgtcta gccgaagatc taggcggaca ggcgctggtt gatctgattc
     6781 gctccgatac gcacacctgc tatctgggtg agcgcggcgc gctgcacgac tggggtcatg
     6841 gctgcggtat ctgtccggcc tgcgatctgc gcgcgcgggg ttatcaacgc tacatcatgg
     6901 ggaaaacagg taatcatgct tagctctgac acaccttaga acaactcgtt ctttatctga
     6961 ttcctgtttt tttcttcttc cggcaaaacc gctacgtttt gactacccag ccccagatca
     7021 tgcacgctgg cgttgggcgg atattccgca taatagtatt cgccgttcga ctcgagcaaa
     7081 ccgctcggca ctgtacgatc gaccatcggc gcactcttca ggactttctg catgtaattg
     7141 atccagatcg gcaaagccag cccgccgcca gtttcgcggt cacccaggct cttcggctgg
     7201 tcaaagccca tccaggcgat accggccaga ttcggctgat aaccggcaaa ccaggcatcc
     7261 atggaatcgt tggtggtgcc agtttttcct gccaggtcgg tacggttgag cgatagcact
     7321 ttgactgccg tgccgtgacg caccacatcg cgcagcatgc tgtccatcac gaacgcatta
     7381 cgcggatcga tcacgcgatt gctttcatcg cccgccctgg ccggtcgcgc ttctgacaat
     7441 accttgccat tgctatcagt gatcttggag atcaggtagg gattgacttt gtaaccgccg
     7501 ttggcgaaca ctgaatacgc aaccaccatc tgcagcggcg tcaccgcacc agcccccagc
     7561 gccagtgtca gataaggcgg attcttgtcg gcatcgaaac cgaaacgggt gatgtattcc
     7621 tgtgcatact tcacgccgac acgttcaagt atgcggatcg ataccatatt gatcgacttc
     7681 tgcaaggcac gacgcatcga gatcggccct tcgaacttgc cgccatagtt ctttggctgc
     7741 cacacctgac cgccggtctg ggtaaacgtg agcggcgcat cgttgatgat ggtcgctggc
     7801 gacaagcctt tttccagcga agatgagtaa ataaaaggct tgaacgacga ccctggctga
     7861 cgccatgcct gaatcacgtg gttgaacttg ttgcggttga aatcgaagcc accgaccagc
     7921 gcgcggatcg cgccatcgtt gggattgagc gcgacgaagg ctgattcgac ttccggcatt
     7981 tgcgtcacga tccagtcttt gccgtcctga aaaacacgaa tgatcgcacc gcgcctgacc
     8041 cgcttttgcg gcggtgcttt ttcactcagc aaattgccgg cgaagcccag accagagccg
     8101 gaaatcctga tttcgtcgcc agtagccagc accgccgtga tttccttgtt tgtcgctttc
     8161 agcaccacgg cggcaaccag gtcatcgctg tcgggatggt cggccagctc ggactcgatg
     8221 gcatcatcag ccgcttcctt ggatgaggtc ggaatctcca tataaccttc cggaccgcga
     8281 tagccatgac ggcgctcata attgatgatg ccgcggcgca gcgccagata ggcagcatcc
     8341 tgatcggtct tggtaatcgt cgtgaagacg ttcaagccgc gcgtgtaagc atcatccttg
     8401 aa
//
LOCUS       DASRDH010000076         3276 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1562762,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000076 DASRDH010000000
VERSION     DASRDH010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 3276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3276
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1562762"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="VFA14_01865"
     CDS             complement(<1..284)
                     /locus_tag="VFA14_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HZS80163.1"
                     /translation="MAEEQKTSDGEDTFISHLIELRSRVVRSAAAVLIIFLCMTPWAA
                     PIFDLLAAPMIHALPAGGRIIAVGVITPFLIPIKVTMLLAFMIALPWVLY"
     gene            complement(327..815)
                     /gene="tatB"
                     /locus_tag="VFA14_01870"
     CDS             complement(327..815)
                     /gene="tatB"
                     /locus_tag="VFA14_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008332430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HZS80164.1"
                     /translation="MIDIGLSKLALIGVVALVVIGPERLPKVARMAGSLFGRAQRYIN
                     EIRVEVSREIELEELRKMRKDMEEMAGAVHSSLSESVADAGSSMHDVWNDGNGEDGDT
                     PSPAPEALLIKAKNFRRKKLARASAASVRHRNGRRARVISAAARVARYRPVGIGKSVG
                     FF"
     gene            complement(853..1059)
                     /gene="tatA"
                     /locus_tag="VFA14_01875"
     CDS             complement(853..1059)
                     /gene="tatA"
                     /locus_tag="VFA14_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HZS80165.1"
                     /translation="MGLSSIWHWLIVLLVIMLLFGTKKLGNMGSDLGKAIKGFKDGIK
                     GEEEKPRQDQTIVDAQAKDKSGHR"
     gene            complement(1098..1481)
                     /locus_tag="VFA14_01880"
     CDS             complement(1098..1481)
                     /locus_tag="VFA14_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HZS80166.1"
                     /translation="MNDCIFCKIAAGKIPSGIFYEDDDVIAFHDIKPAAPVHFLIVPK
                     EHVATLADCGEKHAALLGKMALLAPKLAQEQGCGYRSGAGGDRTGGFRTIFNTGPDSG
                     QEVYHLHMHVIGGPPPWRAGAAPQG"
     gene            complement(1535..1879)
                     /locus_tag="VFA14_01885"
     CDS             complement(1535..1879)
                     /locus_tag="VFA14_01885"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HZS80167.1"
                     /translation="MSEMLHRLAEVIESRKPVNGGDPDQSYVARLFSKGDDAILKKIG
                     EEAAEIIMAAKDARVSGDVSRLLYECADLWFHSMVMLAQFDLEPQDVLDELARREGLS
                     GLEERASRPAGA"
     gene            complement(1876..2328)
                     /gene="hisI"
                     /locus_tag="VFA14_01890"
     CDS             complement(1876..2328)
                     /gene="hisI"
                     /locus_tag="VFA14_01890"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007879643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HZS80168.1"
                     /translation="MGISATWLNKVKWSEVGLVPVIAQEVGSSDVLMFAWMNRDALTR
                     TVQTGEAVYWSRSRKKLWHKGEESGHFQKVHEIRLDCDADVLLLKVEQVAGIACHTGR
                     HSCFFQKFEGNAESGEWRTVEPVLKTPNAVPENGKPERVRKTISKASS"
     gene            complement(2318..3091)
                     /gene="hisF"
                     /locus_tag="VFA14_01895"
     CDS             complement(2318..3091)
                     /gene="hisF"
                     /locus_tag="VFA14_01895"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008332445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HZS80169.1"
                     /translation="MTLAKRIIPCLDVTAGRVVKGVNFVGLRDAGDPVEIARRYDGQG
                     ADEITFLDITASSDGRGLILDIIEAVAAQVFIPLTVGGGVRVVDDVRRLLNAGADKVA
                     INTSAVTNPQLVEDAAGKYGSQCIVVAIDAKRSGAGKWEVFTHGGRNATGLDAVAWAC
                     RMAQLGAGEILLTSMDRDGTRSGFDLALTRAVSEAVAIPVIASGGVGGLQDLADGIRQ
                     GKADAVLAASIFHYGQHTVQEAKRFMADQHIPMRLAHGH"
     gene            complement(3088..>3276)
                     /locus_tag="VFA14_01900"
     CDS             complement(3088..>3276)
                     /locus_tag="VFA14_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HZS80170.1"
                     /translation="PIIASGGVHDIQDVEALCEVQDEGIEAVICGRSIYEGSLDLSSA
                     QERADALNTLPAGTGSVS"
BASE COUNT          728 a         1033 c          828 g          687 t
ORIGIN      
        1 tgatacaaca cccatggcaa agcgatcatg aacgccaaca gcatggtgac cttgatcgga
       61 atcaggaacg gcgtgatcac gccgacggca attattctgc cgccggccgg taacgcatga
      121 atcatcggcg ccgccagcag atcgaaaata ggggcagccc acggcgtcat acacagaaaa
      181 atgataagca cagcagcagc cgacctgact acgcggctgc gtaattcgat cagatgcgaa
      241 atgaaggtat cttcgccatc cgacgttttc tgttcttcgg ccatggaatg actacagggg
      301 agaggtggaa aaccagtcca tcgggatcag aaaaagccga cggatttgcc aatgccgacc
      361 ggacggtatc tggccacacg cgctgccgcg gaaatcacgc gggcgcgacg gccgttcctg
      421 tgccgcaccg acgcggccga ggcgcgcgcc agttttttcc tgcggaaatt ctttgccttg
      481 atgagcagtg cttccggcgc tggcgacggc gtgtcaccgt cttccccatt accgtcattc
      541 cagacatcat gcatagaact cccggcatcg gcgacgcttt ccgagaggct gctgtgaacg
      601 gcgcctgcca tctcttccat gtcctttcgc atcttgcgca attcttccag ctcgatctcc
      661 cggctgactt caacccggat ctcattgatg taacgttgcg cgcggccgaa cagtgaaccg
      721 gccatgcgcg ccaccttagg caggcgctcg ggaccaatca ccaccagcgc aacgacgccg
      781 atcagagcca gcttggaaag accgatatca atcatcgcga aggtagtaat cgaaaaccag
      841 gatcaaaacg catcaccgat gaccggattt gtctttggcc tgcgcatcta cgatggtctg
      901 atcctgacga ggcttttctt cttcaccctt gatgccatcc ttgaagccct tgatggcctt
      961 gccgagatcg gaacccatat taccgagctt cttcgtgccg aataagagca tgatgacaag
     1021 cagaacgatc aaccaatgcc agatactgga caaccccatt cttctctcct aagctgacac
     1081 gcatattctc tatgcaatta tccctgcggc gcagcgccag cgcgccacgg aggggggcct
     1141 ccaatcacat gcatatgaag atggtacacc tcctgaccgc tgtcagggcc ggtattgaaa
     1201 attgtcctga aaccgccggt cctgtcgcca cccgcaccag accggtagcc gcacccctgc
     1261 tcctgcgcca gttttggcgc aagcaaagcc attttgccca ataacgccgc atgtttctcg
     1321 ccgcaatcgg cgagcgtggc gacatgctct ttcggcacga tcaggaaatg caccggcgca
     1381 gccggcttga tgtcgtggaa agcgatgacg tcatcatctt cgtagaagat cccggacgga
     1441 attttcccag ccgcaatctt gcaaaaaata caatcgttca cgatatgctt cctgaaagtt
     1501 cggtaaattg tcacctgccg atgcttattt atttttatgc cccggcagga cgactggctc
     1561 tttcctccag acccgacaga ccttcgcgcc gggccagttc atcgagtaca tcttgcggct
     1621 cgagatcgaa ttgcgccagc atgaccatag aatgaaacca caggtcggcg cactcgtaga
     1681 gcagcctgga aacatcgccg ctgacacgtg catccttggc ggccatgatg atttccgcag
     1741 cttcttcgcc gatttttttc aggatggcgt catcaccttt ggaaaacagt ctggcgacat
     1801 aggactggtc aggatcacca ccattgaccg gtttccgcga ttcgatcacc tcggccagac
     1861 ggtgtagcat ttcactcatg agcttgcctt tgaaattgtt ttgcgcacgc gctccggttt
     1921 tccgttttcc ggcactgcat tgggggtttt caggaccggt tcgaccgttc gccattcacc
     1981 gctctcggca ttgccttcaa acttctggaa aaaacaggaa tggcgaccgg tatggcaggc
     2041 aatacccgcc acctgctcga ccttcagcaa gagcacatct gcgtcacagt cgagacggat
     2101 ttcatgaact ttctgaaaat gaccggactc ttcgcccttg tgccacagct tcttgcgtga
     2161 gcggctccag tagacggctt cgccggtctg gacggttctg gtcagcgcat cgcgattcat
     2221 ccaagcgaac atcagcacat cgctggagcc aacttcctga gcgatcaccg gcaccagccc
     2281 gacttcgctc cacttcactt tattcaacca tgtcgcgcta atgcccatgc gccagcctca
     2341 ttggaatatg ttgatctgcc atgaaacgct tggcctcctg cacagtatgc tggccgtaat
     2401 ggaaaatact tgccgccaac accgcatcgg ccttgccttg cctgatacca tcggcaagat
     2461 cctgcaaacc accgacgccg cccgaggcaa tcacgggaat cgctactgcc tccgacaccg
     2521 cgcgcgtgag cgccaagtca aagccgctgc gagtgccgtc acgatccatg ctggtcaaca
     2581 gaatttcacc cgcaccgagt tgcgccattc tgcacgccca cgccaccgcg tccagcccgg
     2641 tagcattgcg gccaccatgt gtgaacactt cccacttgcc cgcgccgcta cgcttggcat
     2701 cgatcgccac gacgatgcat tgcgaaccgt atttgccagc ggcgtcctct accagttgcg
     2761 gattggtcac cgccgaggta ttgatagcga ccttgtcagc accggcattg agcaaacggc
     2821 gcacatcatc aacgacacgc acaccaccgc cgacggtcag cggaataaag acctgcgccg
     2881 ccaccgcttc aatgatgtcg aggatcagac cacggccatc cgacgatgcc gtgatgtcga
     2941 ggaaagtgat ttcatcggcg ccctgcccat cgtaacggcg tgcgatttca accggatcgc
     3001 cagcatcgcg caagccgacg aaattgacac ccttgacaac ccgcccggca gtcacgtcga
     3061 gacagggaat gatgcgtttt gccagtgtca tgatacgctg ccagtccctg ccggcaatgt
     3121 gttcagcgcg tcggcgcgtt cctgcgccga actcaggtcc aggcttcctt cataaatcga
     3181 acggccgcaa atcacggctt cgataccttc gtcctggacc tcacacaaag cctcgacgtc
     3241 ctggatgtca tgcacgccgc cggaagcgat gatcgg
//
LOCUS       DASRDH010000077         2724 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_148434,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000077 DASRDH010000000
VERSION     DASRDH010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2724
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148434"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..1318)
                     /locus_tag="VFA14_01905"
     CDS             complement(65..1318)
                     /locus_tag="VFA14_01905"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007880055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HZS80171.1"
                     /translation="MNAPLERSLLDTIRAPHFPIYLDYSATTPVDPRVAEKMIPYLRA
                     QFGNPASRSHMYGWTAEKAVEDAREEVARLVNADAREIIWTSGATESNNLAIKGAAQF
                     YKTRGKHIITVKTEHKAVLDTVREMERQGFEATYLQPQDNGLVSVEQIAGVVRPDTIL
                     ISVMLVNNEIGVIQPIPEIGEFCRSRGIIFHCDAAQATGKVRIDLENWKVDLMSFSAH
                     KTYGPKGIGALYVRRKPRVRIEAQIHGGGHERGLRSGTLATHQIVGMGEAFRIAREEM
                     DAELVHVRAMRDRLARGLGEIEETYINGDMENRVPHNLNVSFNYVEGESLIMAIKDIA
                     VSSGSACTSASLEPSYVLRALGRSDELAHSSIRFTIGRFTTEEEIDFTIDLIKAKVDK
                     LRELSPLWDMYKDGVDLSTIQWAAH"
     gene            complement(1340..1840)
                     /gene="iscR"
                     /locus_tag="VFA14_01910"
     CDS             complement(1340..1840)
                     /gene="iscR"
                     /locus_tag="VFA14_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HZS80172.1"
                     /translation="MRLTTKGRFAVTAMIDLALRQGNGPVTLSAISGRQEISLSYLEQ
                     LFGKLRRHQIVESVRGPGGGYNLARKADAVSVADIIIAVDEPLDATQCGGKENCHGPT
                     HPGGPRCMTHGLWTTLNEKMVEYLDSVSLQDLVDQQRRKEQQHLEQAHGTEVDVVVMY
                     RPHAVV"
     gene            2133..>2724
                     /locus_tag="VFA14_01915"
     CDS             2133..>2724
                     /locus_tag="VFA14_01915"
                     /inference="COORDINATES: protein motif:HMM:NF024996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HZS80173.1"
                     /translation="MLSAAVLGAANLCAHAQSNVTIYGIVDTAVRYSTHDNAAGDSKL
                     QLIGGPLSGSQIGFKGKEDLGGGLSALFSLENGFTTDNGALVDSTRLFNREALVGLQG
                     NFGKVTLGRQYALIHNMTASYDVYAMSNLKPIGWQGGNYTMGTRLDNSIRYAGTWSGV
                     TATVGYSAGEVTGDIHRKESKGLSIAYDNGPLHVGGA"
BASE COUNT          606 a          775 c          708 g          635 t
ORIGIN      
        1 ctccttgatc agatcccccc accatgcgct ctatctgtta tggcgggaag ttttcatttt
       61 tgcgttaatg tgcagcccat tggatcgtac tcaggtcgac cccatccttg tacatatccc
      121 acagcggcga cagttcgcgc agcttgtcga ccttggcctt gatcaggtcg atcgtgaaat
      181 caatctcttc ttcagtcgtg aaacggccga tggtgaagcg aatcgagctg tgcgccagtt
      241 catcactgcg gcccagggcg cgcagcacat acgatggttc caggctagcc gaggtgcagg
      301 ccgagcctga cgacaccgcg atgtccttga ttgccatgat cagggattcg ccttcgacat
      361 agttgaagct gacattcaga ttatgcggca cgcggttctc catgtcgccg ttgatgtagg
      421 tttcttcgat ctctccaagc cccctagcca agcgatcgcg catggcgcgg acatggacca
      481 gttcagcatc catctcttca cgagcgatgc ggaaagcctc acccatgccg acaatctggt
      541 gcgtcgccaa tgtgccggaa cgcagaccgc gctcatgacc gccgccatga atttgggctt
      601 cgatgcgcac acgcggcttg cgacgcacgt acaatgcacc aatgccttta gggccgtaag
      661 ttttgtgcgc tgaaaaactc atcaggtcga ctttccagtt ttccagatca atcctgacct
      721 tgccagtcgc ctgcgctgca tcgcaatgga aaatgatgcc cctcgaacgg cagaactcac
      781 cgatttccgg aatcggctgg atcaccccga tttcgttgtt gaccagcatc actgaaatca
      841 aaatagtgtc ggggcgcacg acgccagcaa tctgctcgac gctcaccagg ccattgtcct
      901 gcggttgcag gtaagtcgct tcgaaaccct gacgctccat ctcgcgcacg gtgtccagca
      961 cagctttatg ctcggtcttg accgtgatga tgtgcttgcc cctggtctta tagaattgcg
     1021 ccgcgccctt gatggcaagg ttgttgctct cggtcgcgcc ggaagtccag atgatttcgc
     1081 gcgcatcagc attgaccagc ctggcgactt cctcacgcgc atcttccacg gccttttccg
     1141 cagtccagcc gtacatatgg ctgcgcgaag cgggattacc gaactgcgca cgcaggtacg
     1201 gtatcatctt ctcggcgaca cgcgggtcta ccggtgtggt tgccgagtaa tccagataaa
     1261 tcggaaaatg cggtgccctg atggtgtcca gcaggcttct ttccagaggt gcgttcatat
     1321 atcccatcct aatctccaat taaaccactg catgagggcg atacatgaca accacgtcta
     1381 cttccgtgcc atgtgcctgc tccagatgct gttgttcttt acggcgctgc tgatcgacca
     1441 gatcttgcaa cgacaccgaa tccagatatt cgaccatttt ttcattgagc gtggtccata
     1501 gaccatgcgt catgcagcgg ggaccaccgg gatgagtggg gccatggcaa ttttccttgc
     1561 cgccgcattg cgtagcatcc agcggctcat cgacggcgat gatgatgtcg gctaccgaca
     1621 cagcgtctgc cttacgtgcc agattgtaac cgccgccggg gccgcgcaca gactcgacga
     1681 tctggtggcg acgtaatttg ccaaatagct gctccagata ggacaaagaa atctcttgcc
     1741 tcccactgat ggcagacaac gtgacggggc cgttgccctg gcgcaaagcc aggtcaatca
     1801 tcgcggtcac ggcaaagcgg cctttagtag tcaatcgcat gaaatctcga ctccagaatg
     1861 gttttcagtt tccccctgaa ttcctgaata tttcagtcaa gtatatcaaa gttgagtatt
     1921 tttatcaggt attgagaaat atgaagccaa gcactcatca ttcttgaggg ttgtagcccc
     1981 cccttctttc atgttgtctt gaatgcaaca aaaataaaca tgacactctg tcaatcagtt
     2041 atgatcgcgg catggctttg gcctgagagc agtttggggt tgaaagtgtt gtcattcata
     2101 tccatcacga aggagtaatg atgaagagaa tcatgctatc cgcagcagtg cttggcgcag
     2161 caaacctctg cgcgcatgcg caaagtaatg tcaccatcta cggcatcgtc gatactgctg
     2221 tacgctatag cacacacgac aatgctgctg gcgacagcaa gctgcaactg atcggtggcc
     2281 cgctcagcgg cagtcaaatc ggcttcaagg gcaaggaaga tcttggcggt ggcctgagtg
     2341 ccctgttctc cctggaaaat ggtttcacca ccgacaatgg tgctttagtg gactccacgc
     2401 gcctgttcaa tcgcgaagcg ctggtcggcc tgcaaggcaa tttcggcaaa gtgacgctgg
     2461 gacgtcaata cgctctcatt cacaacatga ccgcgagcta tgatgtgtat gcaatgtcga
     2521 acctgaaacc catcggctgg cagggtggta actacaccat gggtacgcgt ctggataaca
     2581 gcatacgcta tgccggcaca tggagcggcg tcaccgccac tgtcggctac agcgcaggcg
     2641 aagtcaccgg cgatattcac agaaaggaat cgaaaggctt gagtattgcc tacgataacg
     2701 gccccctgca cgtcggcggc gcat
//
LOCUS       DASRDH010000078         8893 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_265123,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000078 DASRDH010000000
VERSION     DASRDH010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 8893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8893
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265123"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..938
                     /gene="guaA"
                     /locus_tag="VFA14_01920"
     CDS             <1..938
                     /gene="guaA"
                     /locus_tag="VFA14_01920"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013212596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HZS80174.1"
                     /translation="TSIRAQVGNDAVILGLSGGVDSSVAAALIHRAIGDQLTCVFVDH
                     GLLRLDEGRMVMDMFSQNLGVEVIHVDAAEQFMGHLAGVTDPEAKRKIIGREFVEVFQ
                     AASGKLKSAKWLAQGTIYPDVIESAGKKGAHTIKSHHNVGGLPETLNLKLLEPLRELF
                     KDEVRELGTALGLPHEMVYRHPFPGPGLGVRILGEVRKEFADLLRRADAIFIEELRNT
                     REANGQSWYEKTSQAFAVFLPVKSVGVMGDGRTYEYVVALRAVQTQDFMTAHWAHLPH
                     ELLGRISNRIINEVRGINRVVYDISGKPPATIEWE"
     gene            1130..1263
                     /locus_tag="VFA14_01925"
                     /pseudo
     CDS             1130..1263
                     /locus_tag="VFA14_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611958.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1267..1407
                     /locus_tag="VFA14_01930"
     CDS             1267..1407
                     /locus_tag="VFA14_01930"
                     /inference="COORDINATES: protein motif:HMM:NF014814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZS80175.1"
                     /translation="MLVCRTFRGWAGGRKPKLTTQQTKEIKRLMSDPGIPVSQIAERY
                     KI"
     gene            1492..4761
                     /locus_tag="VFA14_01935"
     CDS             1492..4761
                     /locus_tag="VFA14_01935"
                     /inference="COORDINATES: protein motif:HMM:NF014442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HZS80176.1"
                     /translation="MYTKKGYDLIHRRRNQWANEEEVRHAWMKGLEEALQIDLDAERA
                     KRDSSYNNVVIEFKGPGLFKGSEASPKFIEATEGRLLKYIQKLAVEQGLDEEDYIGVA
                     VDGDHIGFAQVQGGKIAHQPLMPFSTVSFQMVVDALRANFWRAITSENLAEDFGHLSV
                     TGCEFMQELSDALAESLGQPGHRKIKMFFEEWATLYGQAADLSIQQRKKVSGSLGFEF
                     NGLAHLDLPAKLFVTHTFHSFLMKLIAAEIVAAHGMASSTSLIHELLAIDKDEALIVG
                     LRTDVENGGFFNAVGLHGFVEESIFSWYLDATSKKTTRTALCLAIRTLLAQLSVYRFD
                     TIKKAERSRDVLRDFYQDLVPEELRKSLGEFYTPDWLVEHSVAKLGYNNEKWLTARLL
                     DPTCGSGSFLLEAIEQKRMAALAAGWDASRTVNMLTTTVWGFDLNPLAVQSSRVNFLI
                     AIADLLQKCKGRDIEIPILLADAVYSPAPHPDSDQKVIEYSIGSQQANLQVVLPVGLA
                     RDRELLDQVFQLMGDEVENDTEFPAVAKALVKAGVLTKAQLDEWQGNLKQTYDQVLAL
                     HRQNWNGIWFRIVRNFFWSATAGQFDAIVGNPPWVRWSNLPEAYRNRAKPTCEKYAIF
                     SDTKFHGGNELDISAIITYTTADKWLVEDGRLVFLITQTVFQSPSSQGFRRFRINTTD
                     RLVPLSVDDMKELKPFPDAANKTAVVMFTKKIQGTTTYPLDYRVWLGKPKLDNTGNPK
                     KGRGGQSQRLKSIDHRLHRKDVLELVDVIQMEVNPVSDMMEGAPWAVMKPGHFAAYKP
                     LFGPSTWVNGRKGVTTDLNGVYFVEVIDSNMADKTVKVRTRPQEGKTDLGKPRDFWVE
                     VNSIFPLAKGAGDLHPCYFLPENTLYAFVPNMGIDRASLETAAASYNTHTNPKTFKFF
                     KVYKDFLESRSTFKTRMKGAPFFSIYNVGDYTFSPYKVIWAEMTGDFAAAVVASGVVP
                     GYGPRVYVPDHKLYFADFDRPEPAYYLCGMLHSEIVKEMIEAHNVATNMGDIFKHVNL
                     PRFDAINTEHLKLAELVKQAHGQHDMAARTKTVAQVRAIAAKLIESEIKLRRSAVTSK
                     H"
     gene            4909..5700
                     /locus_tag="VFA14_01940"
     CDS             4909..5700
                     /locus_tag="VFA14_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007878288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HZS80177.1"
                     /translation="MKKEAGGRGAISIQVIERTVSLLDALARHPDPASLKELSVVTGL
                     HSSTAHRILNDLVIKRFVDRSESGAYRLGMRLLELGNIVKSRLSVREAALDFMRALHR
                     KTHQTVNLSVRHSDEIVYIDRAFSECSGMQVVRAIGGRAPLHLTSTGKLFLSIDDPKA
                     VRAYATRTGLAGHNKNSITDLDRLERELSMVRVRGYACDNEELELGVRCMAAGIRDDS
                     GQLVAGLSISAPADRQQGDWVENLMATAAQISSALGFVDPQDVAG"
     gene            complement(5728..6642)
                     /locus_tag="VFA14_01945"
     CDS             complement(5728..6642)
                     /locus_tag="VFA14_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014008266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HZS80178.1"
                     /translation="MWFKNLQIYRLPAPWTTSADQLASYLAPQAFTACSSLEMQSQGW
                     VSPRGNAQLVHVLQRQFLLKLDTEKKLLPSTVINQVTRARAAELEEQQGFPPGRKQIK
                     ELKEQITDELLPRAFSIVRSTWTWIDPVNGWLVVDAGSPAKAEEVLKLLLKAIPKFPL
                     TALRTVQSPLSSMTGWLASDEAPHGFTVDQDTELRASGDSKATVRFVRHTLEPDDVCR
                     HIESGKQCTRLALSWNDRVSFVLTEDLSVKKVVPLDVLKEGSTVAPKNDDERFDGDFM
                     LMAGEIGRLLADLVGALGGYAETTALTA"
     gene            6858..7349
                     /locus_tag="VFA14_01950"
     CDS             6858..7349
                     /locus_tag="VFA14_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin family protein"
                     /protein_id="HZS80179.1"
                     /translation="MKYQHIFLAFPLALTAIGAQSQNMPHHHDSHGDATIGRTGAAAQ
                     VVRTIEVDMNDSMRFSPDSIEVKTGDTIRFIVTNSGRIRHEMVFGTDAQLKSHYVAML
                     KNPGMKHTDANQISLAPGKSGDLIWQFSQAGTISFACLEPGHYDAGMKGSVKVASVGT
                     MHQ"
     gene            complement(7386..8684)
                     /gene="serS"
                     /locus_tag="VFA14_01955"
     CDS             complement(7386..8684)
                     /gene="serS"
                     /locus_tag="VFA14_01955"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007881414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HZS80180.1"
                     /translation="MIDIQLLRKDIDNIAARLAARRFQLDVAAFSALEGERKQIQTRT
                     EELLGKRNNLSRQVGILKGKNEDASAVMAEVSGIGDELKASAARLEEVQSQLSDFLLT
                     VPNLPHASVPAGVDESGNVEVRRVGTPRAFDFEVKDHVDVGAALGLDFNVAARLTGAR
                     FAVMKGGIARLHRALAQFMLDTHTLEHGYTECYTPYIVNADSLRGTGQLPKFEADLFA
                     VRKGGQEGETSDNAALYLIPTAEVPLTNLVRDEIIAGDTLPLKMSAHSPCFRSEAGSY
                     GRDTRGMIRQHQFDKVEMVQVVHPEKSYEALEEMLVHAENILKKLGLPYRVVTLCTGD
                     MGFSAAKTYDIEVWLPAQNTYREISSVSNCESFQARRMQARFRNAQGKPELVHTLNGS
                     GLAVGRTLVALLENKQQADGSVVIPQALRPYMGGLEKLTP"
     gene            8683..>8893
                     /locus_tag="VFA14_01960"
     CDS             8683..>8893
                     /locus_tag="VFA14_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS80181.1"
                     /translation="MEYQMKKCRIRHCIKAIADSSHTAGHSGKKVSPAARLSGVCATI
                     RSHTQESAMSDTSSSPPLITFLIIFA"
BASE COUNT         2027 a         2368 c         2438 g         2060 t
ORIGIN      
        1 tcacatcgat ccgtgcgcaa gttggcaacg atgcagtcat cctcggcctg tctggcggcg
       61 tcgacagcag tgtggcggca gcgctgattc accgcgccat cggcgaccag ttgacctgcg
      121 tgttcgtcga ccatggcctg ctgcgcctgg acgaaggcag gatggtcatg gacatgttca
      181 gccaaaatct gggggtcgag gtcatccatg tcgatgccgc cgaacagttc atgggccacc
      241 tggctggcgt gaccgatccg gaagccaagc gcaagatcat cggccgtgaa ttcgttgaag
      301 tcttccaggc cgcatccggc aaattgaaga gtgccaaatg gctggcgcaa ggcacgatct
      361 atccggacgt catcgaaagc gccggcaaaa aaggggcgca caccatcaag agccaccaca
      421 acgtcggcgg cctgcccgaa acgttgaacc tgaagctgct ggaaccgctg cgcgaactgt
      481 tcaaggatga agtgcgtgaa ctaggcacag cgctcggtct gccgcatgag atggtctacc
      541 gtcacccctt tcccggccct ggtctgggcg tgcgcatcct tggtgaagtc aggaaagagt
      601 ttgccgacct gttgcgccgc gccgatgcga ttttcatcga agaactgcgc aataccaggg
      661 aagccaatgg ccaaagctgg tatgaaaaaa ccagtcaggc atttgcggtt ttcctgccgg
      721 ttaaatcggt cggtgtcatg ggcgacggac gcacttacga atacgtcgtg gcgctgcgcg
      781 ccgtgcagac gcaggatttc atgaccgcgc actgggcgca tctgccgcat gagttgctgg
      841 gtcgcatttc caaccgcatc atcaatgaag tgcgcggcat caatcgcgtg gtctacgaca
      901 tctcaggcaa gccgccggcg acgattgagt gggagtgatt cggcgtctgg cactggctgg
      961 tatcgattag cacaagaaca cagctcagcc cgcgtcactg cgagattttt tgtgatttct
     1021 tccgggcacc gtctggcaat ttttggcatc gcaggtgaat gactgcgacg gcctctatgt
     1081 acctgaacca cctccccgag ttttgagtaa gatctgagag aatggagcca tgaagaagtc
     1141 aaacaaatat tcgctgaagg tacgagaacg tgccataggt atggtgcagg agtattgcag
     1201 cgactatgcg tcgcaatggg ctgcagtcga atcgatagag agctaaagat tggctgtatt
     1261 ccccaaatcc tcgtttgtcg gacattcaga gggtgggccg gtgggcgtaa gccaaaattg
     1321 actacgcagc aaaccaaaga gatcaaacga ctgatgtcag accctggcat tccagttagc
     1381 caaatcgctg aacgctataa aatttaaaga agatctattt acaaggttac acctgtcagg
     1441 caacccagta ctgactgagt gtacctcccc aagtggaagt gcaacatgca tatgtatacc
     1501 aaaaagggct acgatcttat tcatcgtcgt cggaaccagt gggcaaacga agaagaggtg
     1561 cgtcatgcct ggatgaaagg cctagaagaa gccttgcaaa tcgaccttga tgctgaacgt
     1621 gcaaagcggg attccagcta caataacgtc gtaattgaat tcaaaggacc gggcctcttt
     1681 aaaggaagtg aggccagccc caagttcatc gaagcgacgg aagggcgatt gctcaagtat
     1741 atccaaaagc tagctgtaga acagggactc gatgaggagg actacatcgg tgttgctgtt
     1801 gacggcgacc atattggctt tgctcaagtt caaggaggca agatcgccca tcaacctctg
     1861 atgccgtttt ccacggttag cttccagatg gtggtcgatg cattacgggc caatttttgg
     1921 cgcgctatta cttccgaaaa tttggcggag gactttggcc acctatcagt taccggttgt
     1981 gagttcatgc aggagttatc tgatgcattg gcagaatcat tgggtcagcc aggtcatcgc
     2041 aagataaaga tgttcttcga agaatgggcg actctgtatg gtcaagcggc cgatctcagc
     2101 atccagcagc gcaagaaggt cagcggatcg ttgggtttcg aattcaatgg tctggcgcat
     2161 cttgacttgc ccgctaagct cttcgtcact cacacatttc attctttctt gatgaaattg
     2221 atcgcagccg aaatagtggc agcgcatgga atggcatcca gcacgtcgct gatacatgag
     2281 ttactggcta ttgataagga tgaggcgctt atcgtaggac tccggaccga tgttgaaaat
     2341 gggggcttct tcaacgcagt gggtctgcat ggctttgtcg aggagtcaat tttcagctgg
     2401 tatctggatg ccactagcaa aaagaccact cgcacagcat tgtgtttggc gattcgtacc
     2461 ttgctggctc agctctcggt ctatcgcttc gatacaatca agaaggctga acgttcacgc
     2521 gacgtgttgc gagacttcta tcaagatctc gtaccagagg agttgcgtaa gagtctgggc
     2581 gaattttata caccagattg gttggtcgaa cactcagtgg ccaaacttgg gtacaacaac
     2641 gaaaagtggc tcactgcacg ccttcttgac cccacttgtg gatcggggtc gttcctactg
     2701 gaagctatcg agcagaagcg aatggcggca cttgctgcag gttgggacgc cagtcgcacg
     2761 gtgaatatgc tgacgacaac ggtttggggc tttgacctca atcccctggc tgtccaatct
     2821 tctcgcgtca atttcttgat cgctattgcc gatctactgc aaaaatgcaa aggaagagac
     2881 attgagattc cgattttatt ggcggatgca gtgtattcac cagctcctca ccctgacagc
     2941 gatcagaagg tgatcgagta cagcattggg agtcagcaag ccaatcttca ggttgtcctg
     3001 ccggttggtt tagctcgtga tcgtgagctt ttggatcaag tgttccagct tatgggcgat
     3061 gaggtggaaa acgatacgga attccctgcc gttgcgaagg cattagtcaa agcgggagtg
     3121 ttgactaagg cgcaattgga tgaatggcaa ggtaacctca agcagacata tgaccaagta
     3181 ttagctttgc atcgacaaaa ctggaacggt atatggttta ggatcgttcg caatttcttc
     3241 tggtcggcta cagcgggtca gtttgacgcc atcgtaggca atccaccttg ggtccgctgg
     3301 tcaaacttgc cagaggctta tcgcaaccgt gccaagccaa catgcgagaa atatgcaatc
     3361 ttctccgata caaagtttca cggaggtaat gagttggaca tctctgccat cattacctat
     3421 acgaccgctg ataaatggtt ggtcgaagac gggcggctgg tttttcttat cacccagacc
     3481 gtgtttcagt caccatcttc gcaaggtttt cggcgttttc gcatcaatac gacggatcgc
     3541 cttgtgccgt tgtcggtcga tgatatgaag gaactcaaac cgtttcctga cgccgcaaac
     3601 aaaactgcag ttgtgatgtt cactaagaag attcaaggca cgacaacata cccgcttgat
     3661 taccgtgttt ggcttggaaa gccaaagctt gataatacag gcaatcccaa aaagggacgt
     3721 ggcgggcagt cacaacgctt gaagtccatc gaccaccgat tacatcgtaa ggacgtactc
     3781 gaactggttg acgtgattca gatggaagtc aatccggtat cggatatgat ggagggcgca
     3841 ccgtgggcgg taatgaaacc tggccacttt gctgcatata aaccactttt cgggccttcg
     3901 acttgggtca acgggcgtaa gggtgtcacc actgacttga atggtgtcta ttttgtcgaa
     3961 gtgattgatt cgaatatggc agacaagaca gtaaaagttc ggacgcggcc ccaggaaggc
     4021 aaaaccgatc tcggaaagcc acgcgatttt tgggtagagg ttaacagcat ctttccgctt
     4081 gcgaaaggtg ctggcgactt gcatccgtgt tacttcctgc ctgaaaacac gctatacgcg
     4141 tttgttccaa acatgggaat cgaccgtgca tcgctcgaaa cggcagccgc tagttacaac
     4201 acgcatacta accctaagac cttcaaattc ttcaaggtgt acaaggactt tttagagtct
     4261 cgctcaactt tcaagacccg catgaaggga gcgcctttct tctctatcta taacgtcggc
     4321 gattacacat tctcccctta caaggtaatt tgggctgaaa tgacgggtga ctttgcggcc
     4381 gctgtcgtcg catcaggggt ggtgcctggc tatgggccgc gagtctacgt gcccgatcac
     4441 aagctctact ttgctgactt tgatcgccca gagccggcct actacctttg tgggatgctt
     4501 cactcggaga tcgtcaaaga aatgattgag gcccacaatg tggcaacaaa catgggagac
     4561 attttcaagc atgtaaacct accgcgcttc gacgcaataa acaccgaaca tcttaaactt
     4621 gccgagctgg tcaaacaagc gcacggtcag cacgacatgg cagcacgcac caaaaccgtt
     4681 gcacaggtgc gcgcaatagc agccaagttg atcgaatctg agattaagct tcgccgttct
     4741 gcggtcacga gtaagcattg atttcgccga agtgatgtct taaacctaat cgcgataatg
     4801 gggaggagta gagttcggtt gtcatacaaa atcacgtttt atgaacgtgg ttcatttatt
     4861 tttcgcaatg tgctataaca cagcgatcgc aacgcatcaa atttccttat gaaaaaagaa
     4921 gctggcggtc gtggtgccat atcgatacag gtcatcgagc gcacggtgtc tttgctggat
     4981 gcgttggcga ggcatcccga tcctgccagt ctgaaggaat tgtcggtcgt gaccggattg
     5041 cattcttcca cagcccaccg cattctcaac gatctggtga tcaaacgttt cgtggatcgt
     5101 tcggaatccg gcgcttatcg cctcggcatg cgcctgctgg aactgggcaa tatcgtcaag
     5161 agtcgcctga gcgtgcgtga agccgcgctc gatttcatgc gtgcgctgca tcgcaaaacc
     5221 catcagacgg tcaatctgtc ggtgcgtcac agtgatgaaa tcgtctacat cgaccgcgca
     5281 ttttcggaat gctccggcat gcaggtggtg cgtgccatcg gtggccgtgc gccactgcat
     5341 ctgacctcga ccggcaagct gtttctctcc atcgacgatc ccaaggccgt gcgcgcctat
     5401 gcgacgcgta ccggtctggc tgggcataac aagaattcga ttaccgatct ggatcggctc
     5461 gagcgtgaat tgagcatggt gcgtgtccgc ggttatgcgt gcgacaacga agagctggaa
     5521 ctgggcgtgc gctgcatggc agccggtatt cgcgacgatt ccggccagct ggtggctggc
     5581 ctgtccattt ccgcccctgc cgatcggcag caaggggatt gggtagaaaa tctgatggct
     5641 accgctgcgc agatttcttc cgcgctggga ttcgtcgatc cgcaggatgt cgcgggctga
     5701 tgcgcttcct tttcctggac cagtcaatca ggcggtgagc gcagtcgttt ccgcgtaacc
     5761 gcccaatgcc ccaaccagat cggccagcag cctgccgatt tctccagcca tcagcatgaa
     5821 gtctccatca aaacgttcat cgtcattctt cggcgcgaca gtgctgcctt ccttcaggac
     5881 atccagcggc acaactttct tcacgctcag gtcttcggtc agcacgaacg acacgcgatc
     5941 attccagctc agcgccaggc gcgtacattg cttgcccgat tcgatgtggc ggcatacgtc
     6001 gtcaggttcc agcgtatgac gcacgaaacg cacagttgcc ttgctgtcac cgctggcgcg
     6061 cagctcggta tcctgatcca cggtgaaacc gtgtggcgct tcatccgagg ccagccagcc
     6121 ggtcatcgac gacaacggcg actgtacggt gcgcaaagct gtaagcggaa atttcggaat
     6181 cgccttgagc agtagtttca acacttcttc tgctttggcc gggctgccag catccactac
     6241 cagccaacca ttgaccggat cgatccaggt ccaggtactg cgcacgatgc tgaaagcgcg
     6301 cggcagcaat tcgtcggtga tctgttcctt taactccttg atttgtttgc ggcccggtgg
     6361 aaaaccttgc tgttcttcca gttcagcggc acgcgccctg gtgacctgat tgatcacggt
     6421 gctgggcagc agtttttttt cggtgtccag cttgagcagg aactggcgct gtaggacatg
     6481 gaccaattgc gcattgccgc gtggcgatac ccagccctgt gattgcattt ccaggctgga
     6541 gcaggcggta aacgcctgtg gcgcaagata ggatgcaagc tggtcggcgc tggtagtcca
     6601 gggtgcggga aggcgataga tttgaagatt cttgaaccac atggatgttc ggaaaaaatc
     6661 gcccattcta cacgcagtgc gcagtattac tgtacgggag acgtttgccg ggtttggctg
     6721 ctggaatcag caaatcgatc ctgcgttata atggcgcccc gaggaatcct gttcaggcag
     6781 gccgctacgc tgtagttcgc catattgcct gccggctttc ccaccggcat tgcgctctat
     6841 cgatcaagga aaaaaccatg aaatatcagc atatctttct cgcctttccg ctcgcgctta
     6901 ccgccatcgg cgcgcagtcg caaaacatgc cgcaccatca tgacagccat ggtgacgcca
     6961 cgatcggccg taccggcgct gccgcgcaag tcgtccgcac catcgaggtt gacatgaacg
     7021 acagtatgcg attctcgccg gacagcatcg aggtcaaaac cggagacacc attcgcttca
     7081 tcgtcaccaa cagtggcagg atccggcatg aaatggtgtt tggcaccgat gcgcagctca
     7141 agtcacatta tgtcgccatg ctgaagaatc caggcatgaa gcataccgac gcgaaccaga
     7201 tctcgctggc gccgggtaaa agcggtgatc tgatctggca attcagtcaa gcgggcacca
     7261 tcagtttcgc ctgcctggaa ccgggtcatt atgatgccgg catgaaaggc agcgtgaagg
     7321 ttgcctcagt cggcaccatg catcagtagt attgatcgtc tgtccctaag aacgtgaata
     7381 cgctcttagg gcgtcagctt ttcgagtccg cccatatagg gacgcagggc ttgcggaatc
     7441 accacgctgc catctgcctg ctgcttattt tccagtagcg ccaccagcgt gcggccgacc
     7501 gccaggccgg agccgttgag cgtatgcacc agttccggct tgccttgggc attgcgaaag
     7561 cgcgcctgca tgcggcgcgc ctggaagctt tcacagttgg aaaccgatga aatctctcga
     7621 tacgtgttct gcgccggcag ccagacttcg atgtcgtaag tcttggccgc gctgaaaccc
     7681 atgtcgccgg tacacagcgt caccacgcgg tacggcaagc cgagcttctt gaggatgttt
     7741 tcagcgtgga cgagcatttc ttccagcgct tcataggatt tttccggatg tacgacctgg
     7801 accatttcga ccttgtcgaa ctgatgctgg cgaatcatgc cgcgcgtgtc acgaccgtag
     7861 ctgccggctt cggaacggaa gcatggcgaa tgcgcgctca tctttagtgg cagcgtatcg
     7921 ccggcgatga tctcgtcacg caccagattg gtcagcggca cttcagccgt cggaatcaga
     7981 tataaagcgg cgttgtcgct ggtttcgcct tcctgtccgc cttttctgac cgcgaacaga
     8041 tcagcttcga actttggcaa ctggccggtg ccgcgcagcg agtcggcgtt gacgatgtaa
     8101 ggcgtatagc attcggtgta accgtgttcc agtgtgtgcg tgtccagcat gaactgcgcg
     8161 agcgcgcgat gcagacgggc gataccgcct ttcatcacgg caaagcgcgc accggtcagc
     8221 ctggcggcga cgttgaaatc aaggccgagc gcggcgccga catcaacatg atccttgacc
     8281 tcgaaatcga aagcacgtgg tgtgccgacc ctgcgtactt cgacattgcc gctttcatcg
     8341 acgccggcag gcacggatgc atgcggcaga ttgggtacag tcagcaggaa atcactcaat
     8401 tgcgactgca cttcttccag acgcgccgcc gaagccttga gttcatctcc gatgccggat
     8461 acttccgcca tcaccgccga tgcgtcctcg tttttgcctt tgagtatgcc gacctgccgg
     8521 gacagattgt tacgcttgcc cagcaactcc tcggtacggg tctgaatctg cttgcgctca
     8581 ccttccagtg cgctgaaagc ggcgacatcg agttggaatc gacgcgcggc cagacgggcg
     8641 gcgatattat cgatgtcctt gcgaagaagt tgaatgtcga tcatggaata tcaaatgaag
     8701 aaatgccgga tccggcattg tatcaaggcc attgccgatt cctctcacac cgcgggtcat
     8761 tccggtaaaa aagtgtcacc tgccgccagg ctttccggcg tttgcgctac cattcgttct
     8821 catacacagg agtccgccat gtccgacacg tcttcttcac cgccgctgat caccttcctt
     8881 attatttttg ccc
//
LOCUS       DASRDH010000079         3191 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_516715,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000079 DASRDH010000000
VERSION     DASRDH010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 3191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3191
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516715"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..922)
                     /locus_tag="VFA14_01965"
     CDS             complement(<1..922)
                     /locus_tag="VFA14_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017454376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZS80182.1"
                     /translation="MAFPRTFTIPLIVACALFMEQMDATVISTSLPVLAADMGVAPVA
                     LKLALTSYLVSLAVFIPISGWMADRFGALTIFRAAICVFMAGSIFCGISHTLPEFVVA
                     RFFQGIGGAMMVPVGRLVILRSVDKADLVKALGYLTIPALFGPVLGPPLGGFITTYFH
                     WRWIFFINIPISVLGLYLATRFIENIKEAQVAPLDWPGFILSALGCSLLMMGLASAGR
                     HLVPGVVSLACAALGALSLAAYVWHARRTAHPLIDLDLLKITTLLAGVGGGSLFRIGI
                     GAVPFLLPLMLQLGFGFSAFHSGLLTCASAV"
     gene            complement(1063..1491)
                     /locus_tag="VFA14_01970"
     CDS             complement(1063..1491)
                     /locus_tag="VFA14_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008452824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HZS80183.1"
                     /translation="MSFGGFDDRQHVGPMADINVTPMVDVMLVLLVIFILSAPLFTHS
                     LQLDLPTAGSAPAPEKPHTISITIDAAGKLYWQDQAVTLAELASRMQTAAAGQPQPDV
                     QLRADKATRYEVIADVMSAAQNQGLTRLGFVTDPRSAAEK"
     gene            complement(1488..2198)
                     /locus_tag="VFA14_01975"
     CDS             complement(1488..2198)
                     /locus_tag="VFA14_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007881082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HZS80184.1"
                     /translation="MNHDATGQITGFAHYWAQGDAVSHAVAYILLLMSIASWYYILVK
                     TWNSWRIRRSSNALEGFWKAPTLDDAIAVMRAVDSEEIYTPLAQRSSEAAGISSKQQG
                     APSLNAATDPGELITRTLRREINRVSARLERGLTLLASVGSTAPFVGLFGTVWGIYHA
                     LAAVSASGTVQIDKIAGPVGEALIMTALGLVVAIPAVLAYNAFTRINRLTLAELDGFA
                     HDLHAYLTTGERIGKGQA"
     gene            complement(2195..3001)
                     /gene="dapB"
                     /locus_tag="VFA14_01980"
     CDS             complement(2195..3001)
                     /gene="dapB"
                     /locus_tag="VFA14_01980"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HZS80185.1"
                     /translation="MSSLKIAVAGASGRMGRMLIEAIQNADDMILTGALDVPDSPSLG
                     SDAAAFLGRRAGVAITSDLDKGLADAGFLIDFTRPAGTLGHVGYCAAHGVNMIIGTTG
                     LEQEAWDIIAAAAQKTAIVLSPNMSIGVNVTLKLLELAARSFAHGYDIEIIEAHHRHK
                     VDAPSGTALKMGEVIAAAIGVRLDDVAVHAREGITGARDPSSIGFSAIRGGDIVGDHT
                     VLFAGIGERIEITHKSSSRVTYAHGSLRAARFLADKQTGLYDMQDVLGLK"
BASE COUNT          660 a          960 c         1024 g          547 t
ORIGIN      
        1 cgaccgccga cgcgcaggtc aacaggccag aatgaaaggc actgaaacca aagccgagtt
       61 gcagcatcag cggcagcaga aagggaaccg cgccgatgcc gatacgaaac agcgagccgc
      121 cgccgacgcc ggccaacaag gtcgtaatct tcagcagatc gagatcgatc agcggatggg
      181 cggttcggcg cgcatgccac acatacgccg ccagcgacag cgcgccgagc gccgcgcacg
      241 ccagcgacac cacgcccggc accagatgcc gcccggcgct cgccagtccc atcatcagca
      301 acgagcaacc cagcgccgat agaatgaacc ccggccagtc cagcggcgcg acctgcgctt
      361 ccttgatgtt ttcaatgaaa cgcgtggcca gatacagacc aagcacgctg atcggaatat
      421 tgatgaaaaa aatccagcgc cagtgaaaat acgtggtgat gaaaccaccc agcggcggcc
      481 ccagcaccgg cccgaacagc gccggaatcg tcaagtagcc gagtgccttg accagatcgg
      541 ccttatcgac gctacgcagg atcaccagcc gtccgaccgg caccatcatg gcgccgccga
      601 tgccctgaaa gaaacgcgcc acgacgaact ccggcaaggt atgggaaata ccgcagaaga
      661 tggaacccgc catgaacacg cagatcgccg cacggaaaat cgtcagcgcg ccgaagcggt
      721 ccgccatcca gccgctgatc ggaatgaata ccgccagact gactaggtac gaggtcagcg
      781 ccagcttgag cgcaaccggg gcgacgccca tgtccgccgc cagcaccggc agcgaggtcg
      841 aaatcacggt tgcatccatc tgctccatga acagggcgca ggcaacgatc agagggatgg
      901 taaatgtgcg cggaaaggcc atgcgatggg aatacgggca aggcccggat ggacgacgtt
      961 gcagggaatg cgcctcagta taccattcag gcgccaagcg ggcgcaggcc gttcacgcag
     1021 gcttcacgat catccagcaa ggtgactagc gcatgttatg gactattttt ctgccgcaga
     1081 tcggggatcg gtcacgaaac cgagtctggt caggccctgg ttttgcgcgg cggacatcac
     1141 atcggcaatc acttcgtaac gtgtcgcctt gtcggcgcgc agttgcacgt ccggttgcgg
     1201 ctgcccggcg gcggcagtct gcatgcggct ggccagttcc gccagcgtca cggcctgatc
     1261 ctgccaatac agcttgccgg cggcgtcgat ggtgatgctg atggtgtgcg gtttttcagg
     1321 cgcgggcgct gatccggccg tcggcaggtc gagttgcagg gaatgcgtga acaagggcgc
     1381 ggagaggatg aaaatcacca gcagcaccag catcacgtcg accatgggcg tgacattgat
     1441 gtcagccatc ggaccgacgt gctgtctgtc gtcgaaaccg ccgaagctca tgcctgcccc
     1501 ttgccgatgc gttcgccggt agtcaggtaa gcatgcagat cgtgggcaaa gccgtccagc
     1561 tccgccagcg tcaggcgatt gatgcgcgta aaggcgttgt aggccagcac cgcgggaatc
     1621 gccacgacca gacccagtgc agtcatgatg agcgcttcac caaccgggcc ggcgattttg
     1681 tcgatctgca cggtgccgct ggccgagacg gctgcgagcg catggtaaat gccccagacg
     1741 gtgccgaaca ggccgacgaa aggcgccgtc gaaccgaccg aggccagcag tgtcaggccg
     1801 cgctccagac gcgccgagac gcgattgatt tcgcgccgca aggtacgggt gatcagttcg
     1861 ccgggatcgg tggcggcatt caacgagggc gcgccttgct gtttagaaga gatgccggcg
     1921 gcttcggagg agcgctgcgc cagcggcgtg tagatttctt cgctgtcgac cgcgcgcatg
     1981 acggcgatgg catcgtcgag cgtcggcgcc ttccagaagc cttccagcgc attgctgctg
     2041 cggcggatgc gccaggaatt ccaggttttg actaggatgt aataccagct ggcgatggac
     2101 atcagcagca agatataggc gacagcgtgc gacacggcgt cgccctgtgc ccagtagtga
     2161 gcaaatccgg tgatttgccc tgtggcgtca tgattcattt cagccccagc acatcctgca
     2221 tgtcgtatag tccggtctgt ttgtccgcca ggaaacgcgc cgcacgcagg ctgccgtgag
     2281 cgtaggtgac gcggctggag gatttgtgcg tgatttcgat acgttccccg ataccggcga
     2341 acagcacggt atggtcgccg acgatgtcgc cgccacggat cgcggagaag ccgatcgagg
     2401 atggatcgcg tgcgccggtg atgccctcac gcgcatggac ggcaacgtca tcgagcctga
     2461 caccgatggc ggcggcaatg acttcaccca tcttcagtgc ggtgccggat ggcgcatcga
     2521 ccttgtggcg gtgatgcgct tcgatgattt cgatatcata accatgcgca aaactcctgg
     2581 ccgccagctc cagcagcttc agcgtgacgt tgacgccaat gctcatgttc ggtgagagta
     2641 cgatggcggt cttttgggcg gcggcggcga tgatgtccca ggcttcctgc tccaggccgg
     2701 tcgtgccgat gatcatgttg acgccatgcg ccgcgcagta tccgacgtgc ccgagcgtgc
     2761 ccgcgggacg tgtgaaatcg atcaggaaac cggcatcggc caggcccttg tccaggtcgg
     2821 aggtgatggc gacgccggca cgcctgccga gaaaggcggc ggcgtcactg cccaggctgg
     2881 gcgagtcggg gacgtcgagg gcgccggtca ggatcatgtc atcggcgttc tggatggctt
     2941 cgatcagcat gcggcccatg cggccggaag cgccggctac ggcgattttc agggaactca
     3001 taggattgga gactgactta cctgttcgtg atggtgggga cgatcggcgc atcggacagt
     3061 tccggcatgg cgttttttct ggaaccagtg atcagcgaca ggtattcctg ttcggtcggc
     3121 aggtcgttgc cgccttcata gcgatccaga cgatcatcgc tgaagaatac ggatacgcgg
     3181 ctgctcatcc a
//
LOCUS       DASRDH010000080         3154 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1451946,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000080 DASRDH010000000
VERSION     DASRDH010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 3154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3154
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1451946"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1263
                     /gene="fliF"
                     /locus_tag="VFA14_01985"
     CDS             <1..1263
                     /gene="fliF"
                     /locus_tag="VFA14_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008328195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HZS80186.1"
                     /translation="ALEGELARSIQSLSAVQAARVHLALPKASVFVRDQQKPTASVVL
                     NLYPGRMLDSQQVSAIVHLVASSVPSLSPNAVTIVDQNGNLLSDTTTQSSANGLDSTQ
                     LKYVQDLQQDIVKRVESIIAPIVGNGNVRAEATADVDFTRSEQAAETYKPNQAPDTAA
                     VRSKQNSESQNSGSTGASGVPGALSNQPPVPATAPITNPPAAEAPNAANAATQSGASH
                     KDATINYEVDKTVHYVQQPMGGIKRLTVAVVINYKRVETPSGKVTMQPLTEAEKNQIS
                     DLVKEAMGYNKDRGDSLNIVNAQFNSETVPELPLWKQMDMIQLAKEIGKYVLLGAVLL
                     YLYLKVLRPIVTKLTGKEEAAASGGAAADAVSDPNNPDAVVQLSGEAEKLDERHVTYK
                     ANLETAKQLAKSDPKLVASIVKTWVNNG"
     gene            1256..2260
                     /gene="fliG"
                     /locus_tag="VFA14_01990"
     CDS             1256..2260
                     /gene="fliG"
                     /locus_tag="VFA14_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007882580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HZS80187.1"
                     /translation="MGNDSVQKSAILMLALGEDEAAEVMKHLSPHEVQKLGSTMSTMQ
                     NISFEDVNEVLAEFVIASGEASSLGLNSEEYIRSVLKKALGDDKATALLNRILGDRGD
                     AKGIDSLKWMDAQSVAELIRNEHPQIIATILVHLERYHACDVLKCFPERLLNDVVLRI
                     ATLDGVQPAALRELNYVLTTLLTGSEDHRKQQMGGTRTAAEILNFLSGESEKSVMNNL
                     RAYDEGMAQKIEDQMFVFDNIIDIDARGIQLLLREVQSESLIVALKGASEELREKILS
                     NMSQRAAEMMREDLESKGPVRLSEVEAQQKAILQITRRLADEGQIMLGSKGGGGDAFV
                     "
     gene            2338..3027
                     /locus_tag="VFA14_01995"
     CDS             2338..3027
                     /locus_tag="VFA14_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007882581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HZS80188.1"
                     /translation="MASRNNIISKEQMTPYQRWELASFDDIEELPRPDEALFTVEEVP
                     PPPMPTVGEIEAMREQARQEGYAEGQRQGHQEGYAAGLQQGQQETSETIQHLRQIAVS
                     FGAEVSQCSENMAPELLDLSLDLSKAMLKTALSVKPELILPTVNAAIHALPCLQLPAL
                     LYLHPEDVVLVQEHMGEDLSQHGWRIVEDPELERGGCRIETATNQVDTTTQTRWRRIA
                     ESLGRQLNWLE"
BASE COUNT          771 a          893 c          868 g          622 t
ORIGIN      
        1 gcccttgaag gcgaactggc gcgctcgatc cagtccttgt ctgccgtgca ggccgcccgt
       61 gtccatctgg ctttgcccaa agcctccgtt ttcgttcgcg accagcaaaa accgaccgcg
      121 tccgtcgttc tcaaccttta tcctggccgt atgcttgaca gccagcaagt cagcgctatc
      181 gtgcatctgg tggcaagcag cgttccctcg ctgtctccca atgcggtgac catcgtagat
      241 caaaacggta acctgctttc cgacactact acacaatcat ctgccaacgg tctcgattcg
      301 acacagctga aatatgtcca ggatttgcag caggacatcg tgaaacgcgt cgaatcgatc
      361 atcgcaccaa tcgtcggcaa cggtaacgtg cgtgctgaag ctactgccga cgtcgatttc
      421 acgcgcagtg aacaggctgc ggaaacctat aagcccaatc aggctcccga tactgccgcg
      481 gtacgcagca agcaaaacag cgaatcgcaa aacagtggct cgacgggcgc ttccggcgta
      541 cctggagcgt tatctaacca gccgccggtt ccggcgaccg cgccgatcac caatcctcct
      601 gcagctgagg cgcccaatgc tgccaatgcg gcaacgcagt cgggtgcgtc gcacaaggat
      661 gccacgatca actatgaagt cgacaagacc gtgcattatg tccagcaacc gatgggcggc
      721 atcaagcgtc tgacggtcgc cgtcgtgatc aactacaagc gcgtcgagac tccgtctggc
      781 aaggtcacga tgcaaccgct gaccgaggcc gaaaagaatc agatttccga tctggtcaaa
      841 gaagcgatgg gctataacaa ggatcgcggc gactcactta acatcgtcaa cgcgcaattc
      901 aacagcgaaa cggtgccgga actaccgctg tggaaacaaa tggacatgat tcagcttgcc
      961 aaggaaatcg gtaagtacgt gttgctggga gcagtgctgc tatacctgta cctcaaggta
     1021 ctgcgcccca tcgtcacgaa gctcaccggc aaggaagagg ctgcggcgtc tggcggagcc
     1081 gcagcagacg ccgtgtccga ccccaataat ccagatgctg tggtgcagtt gtccggcgaa
     1141 gcggagaaac tcgacgaacg acatgtcact tacaaggcca atcttgaaac agccaagcaa
     1201 ttggcaaaaa gcgatcctaa actggtagca agcatcgtta agacatgggt gaacaatggg
     1261 taacgacagt gttcaaaaaa gtgccatctt gatgttggcg ctgggtgagg acgaagccgc
     1321 cgaggtcatg aaacatctca gcccgcacga agtgcaaaag ctcggctcga ccatgtcgac
     1381 catgcagaac atcagttttg aagatgtcaa tgaagtactg gccgaattcg tcatcgcaag
     1441 cggtgaggct tcttcacttg gcctcaattc cgaggaatac atccgtagcg tgctaaagaa
     1501 ggcgctcggc gacgacaagg ctactgctct gctcaaccgc attctgggtg accgcggtga
     1561 tgccaaaggc atcgatagtc tgaagtggat ggatgcacaa tcggtggccg aactgatccg
     1621 caacgaacat ccgcagatca ttgccaccat cctggtccat cttgagcgtt atcatgcctg
     1681 cgacgtcctt aaatgcttcc ccgaacgcct gctcaatgac gtggtcctgc gtattgccac
     1741 cctcgatggc gtgcaaccgg cggcgttgcg cgagttgaat tatgttctga ccacattgct
     1801 gaccggcagc gaagaccaca gaaaacaaca aatgggcggt actcgcaccg cagcggaaat
     1861 cctcaacttc ctgtcgggcg agagcgaaaa gtcggttatg aacaatctgc gtgcctatga
     1921 cgagggtatg gcgcaaaaga tcgaggacca aatgttcgtg ttcgacaaca tcattgacat
     1981 cgatgcccgc ggcatccagt tgctgttgcg cgaagtccag tctgaatcgc tcatcgtcgc
     2041 gctcaaaggc gcatcagagg agctgcgcga aaagatcctt agcaacatgt cgcaacgtgc
     2101 ggcggaaatg atgcgagaag atctcgaatc caaggggccg gttcgcctgt ctgaagtcga
     2161 ggcacagcaa aaggccatcc tgcaaatcac tcgtcgtttg gccgatgaag gccagatcat
     2221 gctcggttcg aagggcggag gcggagatgc attcgtttaa ggtcgccgcg accaatgtac
     2281 atgatctgcc cggtcgtgcc gcatcgatga cgcttaccta acgttggcgg gtctgcaatg
     2341 gcgtcgcgaa ataatatcat ttccaaagaa cagatgacgc cttaccagcg ttgggaactg
     2401 gcttctttcg acgatattga agaattgccc aggcccgatg aggcgttatt taccgtcgaa
     2461 gaagtaccgc cgccaccgat gccgacggta ggagaaatcg aggccatgcg cgagcaggca
     2521 cgccaggaag gctatgctga aggccagcgc caaggccacc aggaaggcta tgctgccggt
     2581 ttgcagcaag gccagcagga aaccagtgaa accatccaac atttgcgtca gattgccgtc
     2641 tccttcggcg ctgaagtctc gcagtgcagc gaaaacatgg caccggagtt gctcgattta
     2701 agcctggatt tgtccaaggc catgctcaag actgcgctga gcgtgaagcc ggaattgatt
     2761 ctgccgactg tgaacgctgc catccatgcg ctgccctgcc tgcaactgcc ggcgttgctg
     2821 tatttgcacc cagaggatgt cgtgctggta caggaacata tgggagaaga cctcagccag
     2881 cacggctggc gtatcgtcga agatcctgaa cttgagcgcg gcggttgccg catcgagacc
     2941 gctaccaacc aggtcgatac caccactcaa acacgctggc gccgcattgc ggagtccctc
     3001 ggcaggcaac ttaactggct ggagtagttg catgtgcctt cccggccact tccgctcaca
     3061 ggtgcaacga tgaattcacc gatgccctct catttcagtc gctggcaagc ctatctgcat
     3121 gattgtagca agctgctgga aatggccgag ccga
//
LOCUS       DASRDH010000081        10016 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_959042,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000081 DASRDH010000000
VERSION     DASRDH010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 10016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10016
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_959042"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1223)
                     /locus_tag="VFA14_02000"
     CDS             complement(<1..1223)
                     /locus_tag="VFA14_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZS80189.1"
                     /translation="MSQVPADNTNEPQQNQYGASSIQILEGLEAVRKRPGMYIGDTSD
                     GTGLHHLAFEVLDNAIDEALAGHCSEIHVTIHADNSISVTDNGRGIPTDIKMDDKHDP
                     KRSAAEIVMTELHAGGKFDQNAYKVSGGLHGVGVSCVNGLSKLLRLIVRRNGKVHAME
                     FVRGIPQNREIATINGVQVSPIKVIGDTDKRGTEVHFWADEEIFTHVEFHYEILAKRI
                     RELSFLNNGVHIRLTDQRNGKEEDFAFEGGTRGFVEYINKNKNVLNQTIFQATGEKMS
                     DQNTSISVDVSMQWNDAYNEQVLCFTNNIPQRDGGTHLTGLRAAMTRVINKYIEENEL
                     AKKAKVEVSGDDMREGLTCVLSVKVPEPKFSSQTKDKLVSSEVRGPVEEIVAKTLTDF
                     LQEKPNDAKVICSKIV"
     gene            complement(1273..2382)
                     /gene="dnaN"
                     /locus_tag="VFA14_02005"
     CDS             complement(1273..2382)
                     /gene="dnaN"
                     /locus_tag="VFA14_02005"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HZS80190.1"
                     /translation="MQLVKTQRDALLRPLQIVSGIVERRHTLPILANILIRKDGEKVS
                     FLSTDIEVQITTHAEVGFGSEVTAITVAARKLLDILRALPDSGDDVSVTLSNKRLAVQ
                     SGKSRFALQTLAAEEFPTVAQAEHYNAQVTLQQKILKHLFDMVHFSMAQQDIRYYLNG
                     LLLVVEGRSVIAVATDGHRLAVCQVAADREFPRQEVIIPRKTIIELQRLLEESDEPVQ
                     LDIANSQVKFTFAGIELISKLVEGKFPDYTRVVPKGYKNNFTIGREQLLRSLQRTAIM
                     TSDKFKGVRWLIAPGSLKISSTNADQEEAVEELEIGYGGDSVDIGFNVTYLLDVLNNL
                     KGDNVNIALGDANSSALITIPDNTDFKYVVMPMRI"
     gene            complement(2520..3917)
                     /gene="dnaA"
                     /locus_tag="VFA14_02010"
     CDS             complement(2520..3917)
                     /gene="dnaA"
                     /locus_tag="VFA14_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011979227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HZS80191.1"
                     /translation="MENFWLACSAKLEQELTPQQYSAWIKPLAPLDYKDDYLRIAAPN
                     RFKLDWVKTQFAGRITAIASEYWGTRIEVQFVLDPRVGAARRTASAVAAAAPADQYEG
                     HGGGYPAGIQESITEPAPVTLRQEQSRINSVLTFDNLVTGKANQLARAAATQVANNPG
                     VSYNPLFLYGGVGLGKTHVIHAIGNQVLTDNPTSRIRYIHAEQYVRDVVTAYQRKGFD
                     DFKRYYHSLDLLLIDDIQFFGGKNRTQEEFFYAFEALIAAKKQIIITSDTYPKEISGM
                     DDRLISRFDSGLTVAIEPPELEMRVAILMKKAMQEGVQLSDDIAFFVAKHLRSNVREL
                     EGALRKILAYSRFHGKDITIDIVKDALKDLLSVQNRQISVENIQKTVADFFSIKVADM
                     YSKKRPANIARPRQIAMYLAKELTQKSLPEIGDLFGGRDHTTVLHAVRKIAVDRTKNP
                     ECNHELHVLEQTLKG"
     gene            4305..5288
                     /locus_tag="VFA14_02015"
     CDS             4305..5288
                     /locus_tag="VFA14_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZS80192.1"
                     /translation="MSFLTLDLNLLRVFDAVMTEQNLTRAANLLAMTQPAVSNALRRL
                     RETLNDKLLIRTAHGVKPTPRAEELWPTVRRALSDLEAVITPETFDIAHAQNTFRMAM
                     VDTTAVLWLPALVRTIMCDAPHIKIRMMPLTTRDPRAMLLRGDVDLAVGFFPGVTVQL
                     GDRPKTQINHTRLYTGRYVCVMHKHHPLADKELDIDNYCSANHLLVSLSGKPYTVIDN
                     VLADMGRERKVLLTVNQFFTAGRIVASSDLITVLPHHLIAATGMQEVLVVKELPFETP
                     DEHVDMLWHERDARNPAHKWLRAHLSATTSDEFGRITNSNDMSHVKRNKET"
     gene            5289..6155
                     /locus_tag="VFA14_02020"
     CDS             5289..6155
                     /locus_tag="VFA14_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014631989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HZS80193.1"
                     /translation="MSPKTLRLNLPQWQGGNEPTYRFGAELLRWLAPAHDGPEETVEV
                     PVAGEQPLEIEQGIKGRAALLSQARAARTAIERHQPDRIVTLGGDCLVDLAPMAYLNR
                     RYDGKLGVLWIDAHPDVMNAAEFSHAHAHVLGMLLGRGDADFTAEVERKLSPSRVMIA
                     GMNSWTKVEDPILQELGIRHTPVAGIADSSQPILDWIKAEDITHLAVHFDLDVLDATH
                     FSPLLFNQPEQPEGAFDGIVQGSMRLEQVVRLLQDVSSACDMVGLAIAEFLPWDMLRL
                     KNALVSLPIMQR"
     gene            complement(6156..7463)
                     /locus_tag="VFA14_02025"
     CDS             complement(6156..7463)
                     /locus_tag="VFA14_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HZS80194.1"
                     /translation="MSDLFAREPVAPLAEALRPQTLDEVVGQSHLLGEGKPLRLAFES
                     GKPHSMILWGPPGVGKTTLARLTATAFKCEFIALSAVFSGVKDIRAAMEQAEQSLAMG
                     KHTILFVDEIHRFNKSQQDALLPYAESGLVIFIGATTENPSFEVNSALLSRAQVYVLK
                     PLTEDELKQLVTKAQRQVLSHLQFQQAAIDALAVYADGDARRILNLLEQTSTAARTAG
                     TTRITSEFIRNALAPHTRRFDKGGDDFYDQISALHKSVRGSSPDAALYWLCRMLDGGA
                     DARYLSRRIVRMAWEDIGLADPRAMQIANDAAFTYERLGSPEGELALGQAVIYLAIAA
                     KSNAGYNAFNQAMAFVKQDKSREVPVHLRNAPTKLMKELGYGHAYRYAHDEPYAYAAG
                     ETYLPDGMQDPDWYEPVARGLESRIAEKMAFLRKLDEEAGKSR"
     gene            complement(7527..8192)
                     /gene="lolA"
                     /locus_tag="VFA14_02030"
     CDS             complement(7527..8192)
                     /gene="lolA"
                     /locus_tag="VFA14_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HZS80195.1"
                     /translation="MKAETLSGSSARQCALAMVALGFCAMLFSAQAVAGALEQFSLFV
                     SSTQSARGEFVQRLVKTDSGAVKVLNISSGVFVFSRPGKFIWTYQKPYEQVLQADGDK
                     LFIFDKDLNQVITKKLGNALGSSPAAILFGSNDLEQNFILKEGGARDGMEWLEATPRS
                     KDTSFDRIGIGLKDGVPQAMELRDSFGQTALLSFGRFERNPPLKSGSFTFVVPEGVDV
                     FNN"
     gene            complement(8202..>10016)
                     /locus_tag="VFA14_02035"
     CDS             complement(8202..>10016)
                     /locus_tag="VFA14_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007881407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HZS80196.1"
                     /translation="SSLGFTGATLLLLLLFGLGVSLFFHVSWLEIAERIGGFIEDGIC
                     WVRSRYLARQDRKVGRVAAVRREEAVVQERARIVEAQPIHIEPQVVAVPKSGRTGKER
                     QTSLFEGMDSSLPPLSLLDDAPPAQQTMSVETLEFTSRLIEKKLSDFGVTVKVVAAYP
                     GPVITRYAIEPATGVKGSQIVGLARDLARSLSLTSIRVVEVIQGKNYMGLELPNTKRQ
                     IVRLTEILDSKVYNDSRSNLTVALGKDIAGNPVVADLARMPHLLVAGTTGSGKSVGIN
                     ATILSLLYKSTSRQVRLILIDPKMLELSIYEGIPHLLAPVVTDMRQAGHALNWAVAEM
                     ERRYKRMSKLGVRNLSGYNQKITDAETRGEKIPNPFSLTPDAPEPLEQLETIVIIIDE
                     LADLMMVVGKKVEELIARIAQKARAAGIHLILATQRPSVDVITGLIKANVPTRIAFQV
                     SSRVDSRTILDQMGAEALLGMGDMLYMPPGTGLPIRVHGAFVSDEEVHRVVDYLKAQG
                     EPDYIEGILEGGVAGDAEGVVVGDTAGGEGDELYDQAVAVVLKNRRASISLVQRHLRI
                     GYNRAARLLEQMEQSGLVSTMQSSGNREILVPVGNVAE"
BASE COUNT         2191 a         2836 c         2782 g         2207 t
ORIGIN      
        1 acgatcttgc tgcagatgac cttggcgtcg ttcggttttt cctggagaaa atcggtgagt
       61 gtcttggcga cgatttcttc gaccgggcca cgtacttcac tggagaccag cttgtctttg
      121 gtctgcgagc tgaacttggg ctcggggact ttgacggaga gtacgcaggt gagaccttcg
      181 cgcatgtcgt ctccggagac ttcgaccttg gcttttttgg caagctcgtt ttcttctatg
      241 tatttgttga tgacgcgggt catcgcagca cgcaagccgg tcaggtgggt accgccgtcg
      301 cgctgcggga tgttgttggt gaagcagagc acctgttcgt tgtaggcatc attccattgc
      361 atggagacat cgacgctgat gctggtgttc tgatccgaca ttttttcacc ggtggcttga
      421 aagatggtct ggttgagaac gttcttgttc ttgttgatgt attcgacgaa accgcgggtg
      481 ccgccttcga aggcaaaatc ttcttccttg ccattgcgct ggtcagtgag cctgatatgc
      541 acgccgttgt tgaggaagga cagttcacgg atgcgcttag ccagaatctc gtagtgaaat
      601 tcgacgtggg taaagatttc ttcatcagcc cagaaatgga cttcagtgcc gcgcttgtcg
      661 gtgtcgccga tgactttgat cggagagacc tgcacgccgt tgatggtcgc gatttcgcgg
      721 ttttgcggaa tgccgcgcac gaattccatg gcatgtactt tgccgttgcg gcgaacgatc
      781 agacgcagca gtttggacaa gccgttgaca caggagacgc caacaccatg caggccgccg
      841 gagaccttat aagcgttctg gtcgaacttg ccgccggcgt gaagttcggt catgacgatt
      901 tcggcggcgc tgcgttttgg atcatgcttg tcgtccatct tgatgtcggt cggaatgccg
      961 cggccgttat cggtgacgga aatcgaattg tcggcatgga tggtgacgtg aatttcgctg
     1021 caatggccgg cgagcgcttc atcgatggca ttgtccagaa cctcgaacgc gaggtggtgc
     1081 aaaccagtgc cgtcagaggt gtcgccgatg tacatgccgg gacgtttgcg gactgcttcc
     1141 agaccttcca gaatctggat ggaggaggct ccatattgat tttgttgcgg ttcattggtg
     1201 ttgtctgctg ggacttggga catggctgcc ttctgaaact gatggaactg cctaattcgt
     1261 tatcgccgga catcaaatgc gcatcggcat gacaacatat ttgaaatcag tattgtcggg
     1321 gatggtgatc aacgcagaag agttggcgtc acccagagcg atgttgacat tatcgccttt
     1381 gaggttattc aacacgtcga gcagataggt gacattgaag ccgatgtcta cgctgtcgcc
     1441 gccgtagccg atttcgagtt cttcgaccgc ttcttcctgg tctgcgttgg tggaactgat
     1501 cttgagactg cccggagcaa tcaaccagcg cacacccttg aacttgtcac tggtcatgat
     1561 ggcagtacgt tgcagcgagc gcagcaattg ttcacggccg atggtgaagt tgtttttgta
     1621 acccttgggc accacgcggg tgtagtcagg gaatttgcct tcgaccagct tggaaatcag
     1681 ctcgataccg gcaaaagtaa acttgacctg actgttggca atgtcgagct ggaccggctc
     1741 atcgctttct tcgagcaggc gttgcagttc gatgatggtc ttgcgcggga tgatgacttc
     1801 ctgacgcgga aattcccgat cagcggcaac ctggcaaaca gccagacggt ggccgtcggt
     1861 ggcaacggcg atgacgctcc ggccttcgac gaccagcagc aggccgttga ggtaataacg
     1921 gatgtcttgc tgcgccatcg agaaatggac catgtcgaac aggtgcttca ggatcttttg
     1981 ctgcaacgtg acttgcgcgt tgtaatgctc ggcctgggcc accgtcggga attcttccgc
     2041 agccagggtt tgcagggcaa aacgcgattt gccggactgg actgccagac gcttgttgga
     2101 caacgttacc gaaacatcat cgccagaatc gggcaaggcg cgcaggatat cgagcagttt
     2161 gcgcgcggcg acggtaatcg cggtaacttc gctaccgaag ccgacttcag catgtgtggt
     2221 gatctgcact tcgatatcag tagagaggaa agatactttt tcgccatcct tgcgaatgag
     2281 gatattggcc agaatcggca atgtgtgccg acgctcgaca ataccgctca cgatttgcag
     2341 tggtcggaga agtgcatctc gttgggtttt gaccaattgc atagtctcta tgtccttact
     2401 ggtaaatggt ttaggatact gaaagccgct ttcgctttta cctgtcgatg acaacgaaag
     2461 cgccgccggg accgggccaa actgctcatt gtaccaacac cagcagattg accattgggt
     2521 caacccttga gagtctgctc aaggacatga agttcgtggt tacattccgg attcttggtg
     2581 cgatcgacgg cgatcttgcg tactgcatgc agcaccgtgg tgtgatcacg accaccgaac
     2641 aggtcgccaa tttccggcag actcttttgc gtcagttcct tggccaggta catggcgatc
     2701 tggcgtggac gagcgatatt ggccggacgc tttttggaat acatgtcagc gactttgata
     2761 ctaaaaaagt cggcaacggt tttctggatg ttttcgacgg aaatctgcct gttctgaacc
     2821 gacaggagat ctttcagcgc atctttgacg atgtcaatgg tgatgtcctt gccgtggaag
     2881 cgcgagtagg ccaggatctt gcgcaaggcg ccttcaagct cgcgcacgtt ggaacgcaaa
     2941 tgcttggcga caaagaaggc gatatcgtca gacagctgca caccttcctg catggctttt
     3001 ttcatcagaa tggcaacacg catttccagc tcgggcggct cgatggcgac cgtcaggccg
     3061 gaatcgaagc gcgaaatcag acggtcgtcc atgccgctga tttccttggg ataggtgtcg
     3121 ctggtgatga tgatctgctt ttttgcggcg atcaacgctt caaaagcgta gaaaaactct
     3181 tcctgggtgc ggttcttgcc gccgaagaat tgaatatcgt cgatcagcag cagatcaagc
     3241 gagtggtaat aacgcttgaa gtcatcaaaa cccttgcgct gataagccgt gactacgtcg
     3301 cgcacatatt gctcggcatg gatataacgg atcctggagg tcggattgtc ggtcagcacc
     3361 tggttgccaa tggcgtgaat gacatgggtc ttgccaaggc cgacgccgcc gtacagaaac
     3421 agcgggttat aggacactcc gggattgttg gcgacctggg tggcggcagc gcgcgccaac
     3481 tggttggcct tcccggtgac gaggttgtcg aaagtcagca ccgaattgat gcgcgactgt
     3541 tcctggcgca gtgtgaccgg cgccggttca gtgatggatt cctggatgcc ggccgggtaa
     3601 ccgccgccat gtccctcata ctgatctgcc ggcgcagcag cggctactgc actggcggta
     3661 cggcgagcgg cgccgacgcg cgggtcgagc acgaactgga cttcgatccg ggttccccaa
     3721 tattcggaag caatagccgt gatgcgacca gcgaactggg ttttgaccca gtcgagtttg
     3781 aagcggttgg gcgcagcaat acgcaggtag tcatctttat aatcaagcgg cgcgagcggc
     3841 ttgatccagg cgctatattg ctgcggcgtc agctcttgct ccagctttgc agaacaggcc
     3901 agccaaaaat tttccatgaa tcttcttata aaaaacaggg gagcgggaaa cgtgtcggac
     3961 cgactttggc aaggctgaat gccgacacca cgcaagcgcc tattttactc ctgccagcaa
     4021 aggttatcca caggcaggag cattttcgac agagcaaaaa tgcatattga tcattggata
     4081 aaagcactgc cggaaatatc gacaaaatat taacgcaata aatgaattct tcgatccatg
     4141 ccgtcataat ggacccttta ctgagtgtgt tatgctctgt tgcgctgtca tgatccagaa
     4201 cctgcctgat cagtttcctt ttcagacgtc agccataggt ggttgtgaca tgcctgccct
     4261 cttttatccc cgattaccgg cgttccttat aaaacatagc tgacatgagc tttctgacgc
     4321 tcgatctgaa cctgttacgc gttttcgatg cggtgatgac cgagcaaaac ctgacacgcg
     4381 ccgccaatct gctggcgatg acgcaaccgg ccgtatccaa cgcgctgcgc cgcctgcgcg
     4441 aaacgctcaa tgataaacta ctgatccgta ccgcccatgg cgtcaagccg acgccgcgcg
     4501 cagaagagct gtggccgacc gtgcgccgcg ctctgtcgga tctggaagca gtgatcacac
     4561 cggaaacctt cgacatcgcc cacgcgcaaa acactttccg catggcgatg gtcgatacca
     4621 ctgccgtgct atggctaccg gcactggtac gtaccatcat gtgcgatgcg ccgcacatca
     4681 agatccgtat gatgccgctg accacacgtg atccgcgcgc catgctgttg cgtggcgacg
     4741 ttgatctagc tgtggggttt ttcccgggcg taacggtcca gctgggagac cggccgaaaa
     4801 cccagatcaa ccacacgcgc ctatacactg gccgatacgt ctgcgtgatg cacaaacatc
     4861 atccgctagc cgacaaggaa ctcgatatcg acaattactg ctctgctaac catttactgg
     4921 tcagtctatc cggcaaacct tataccgtca tcgataatgt actcgccgac atggggcgtg
     4981 aacgcaaggt tctgctaacc gtgaaccagt tctttacagc cggtcgcatc gtcgccagtt
     5041 cggatctcat cactgtactg ccgcatcacc tgattgccgc caccgggatg caggaagtcc
     5101 tggtagtaaa agaactgcca ttcgaaacgc ccgacgaaca tgtcgatatg ctgtggcatg
     5161 agcgtgatgc ccgcaatcca gcgcataaat ggttacgcgc gcacttgagc gcaacgacca
     5221 gcgacgaatt cggtcgcatc actaatagca acgacatgag tcatgtgaaa cgcaacaagg
     5281 aaacgtgaat gtcgcccaaa acgctccgac tgaacttgcc ccagtggcaa ggaggcaatg
     5341 aacccacata ccgttttggc gccgagttac tacgctggct ggctcctgcg cacgacgggc
     5401 cggaagaaac ggtagaggtt cccgttgccg gtgaacagcc gctggaaatc gagcaaggca
     5461 tcaaggggcg cgcagcactg ctatcgcagg cccgtgccgc acgaacagcc atcgagcgac
     5521 atcaacccga ccgcatcgtc acgctgggcg gcgattgcct cgtcgacctc gcccctatgg
     5581 cctatctgaa tcgccggtat gacggaaaac tgggagtgct atggatcgat gcccaccccg
     5641 atgtcatgaa cgctgccgag ttctcgcacg cccatgccca cgtactgggc atgttactgg
     5701 ggcgcggcga tgcggacttc acagccgagg tcgaacgcaa gctaagccca tcgcgcgtga
     5761 tgatcgccgg catgaactcc tggacaaagg tcgaagaccc cattcttcag gaactgggca
     5821 tccggcacac gccagtcgcc ggcattgccg acagcagtca accgatcctg gactggatca
     5881 aggccgaaga catcacccac ctggccgtgc attttgatct ggacgtactc gacgccaccc
     5941 acttctcacc gctgctgttc aaccagcctg aacagccgga aggcgcattc gacggcatcg
     6001 tgcaaggctc gatgcggctg gagcaagtcg tccggctatt acaggacgtg agcagcgcct
     6061 gcgatatggt cggactggcg atcgcggagt tcctgccttg ggacatgcta cgcctcaaga
     6121 atgcactggt cagcctgccc atcatgcagc gctgattacc tgctttttcc tgcctcttca
     6181 tccagtttgc gcaagaaagc cattttctcg gcgatcctgc tctccagacc tcgcgccact
     6241 ggctcatacc agtctgggtc ttgcatgccg tccggcagat aggtttcgcc ggcggcatag
     6301 gcatacggtt cgtcatgggc ataccggtac gcatggccat agcccagttc cttcatgagc
     6361 tttgtgggag cgttgcgaag gtgaacagga acttcccgag acttgtcctg cttcacaaat
     6421 gccatcgcct ggttgaaggc gttataacca gcattgcttt tggcagcaat tgccagatag
     6481 atgacggctt gcccaagagc cagctcgcct tccgggctgc caaggcgctc ataggtaaat
     6541 gctgcatcat tggctatctg catggctctt ggatcggcca gtccgatatc ctcccatgcc
     6601 attctcacaa tacggcgaga caggtagcgg gcatcggctc caccgtccag catacgacat
     6661 agccagtaga gagcggcatc agggctggag ccacgcactg acttgtgaag ggcggaaatc
     6721 tggtcataga aatcatcccc tcctttatca aaccgtctgg tatgcggggc gagggcattc
     6781 cggatgaact cggaggtgat cctggtggtg cccgccgtcc tggctgcggt gctggtctgt
     6841 tcgagcagat tcagtatacg acgagcatca ccatccgcat aaacagccag agcatctatc
     6901 gctgcctgct gaaactggag atgcgaaaga acctgccgtt gcgcctttgt caccagttgc
     6961 ttcagctcgt cttcggtgag tggtttgagg acatagacct gagcacggga aagcagtgcg
     7021 gaattgactt caaacgaagg attctccgtt gtggctccaa tgaagatcac cagaccagac
     7081 tcggcataag gcaacagagc gtcttgctgg gacttattga acctgtgaat ttcgtccacg
     7141 aacaaaatgg tgtgcttgcc catggcaagg ctctgttctg cctgctccat ggcggcacga
     7201 atatctttga cgccagaaaa gacagcagac aaggcgatga attcgcattt gaaagcagtg
     7261 gcagtcaacc gcgccaatgt cgtttttcca acaccaggcg gcccccaaag gatcatggaa
     7321 tgcggcttgc ctgactcaaa agcgaggcgt agcggcttgc cctccccaag cagatgggat
     7381 tgcccgacga cctcatccag ggtttgggga cgcagggctt cagccagcgg ggcaacaggc
     7441 tcgcgcgcga acaaatcaga catcatcaaa gaagtgtagg caacaatgtg gtctgcaacg
     7501 aaatcgacct gcccggaact gccatttcag ttattgaaaa cgtcgacgcc ctcaggcacg
     7561 acaaaagtga agctgccgga cttcagcggc ggattccttt caaacctgcc aaacgacagc
     7621 agcgcagtct gaccgaagga atcacgcagc tccatcgcct gcggcacgcc gtccttcaag
     7681 ccgatgccaa tgcgatcgaa gctcgtatcc ttgctcctgg gtgtggcttc cagccattcc
     7741 atgccatccc tggcgccgcc ttctttcagt atgaaattct gttccagatc gttactgcca
     7801 aacaggatcg cggccggcga cgagccgagc gcgttgccta atttcttggt gatgacctga
     7861 ttcagatcct tgtcgaaaat gaacagtttg tcaccatccg cctgcaagac ctgctcgtag
     7921 ggcttctggt aggtccagat gaattttccc ggacgcgaaa acacgaagac gccactcgat
     7981 atattcaaca ccttgacagc gccactgtcg gtcttgacca ggcgctggac gaattcgccc
     8041 ctggctgatt gcgtggacga cacgaacagg ctgaactgct ccagcgcgcc cgccacagcc
     8101 tgcgcggaaa aaagcatcgc acagaatccc aacgccacca tagcgagcgc gcactgccgc
     8161 gcgctcgaac ctgataatgt ttctgccttc atttgcatcg attactccgc gacatttcct
     8221 accggcacca ggatttcacg gttgccgctc gattgcatgg ttgacaccag accgctttgc
     8281 tccatctgct cgagcagacg cgcggcacga ttgtaaccga tacgcaaatg gcgctgcacc
     8341 agagagatcg atgcccgacg gtttttcagc accacagcga cggcctgatc gtagagttca
     8401 tctccttcgc cacctgcggt atcaccaacg actacccctt cagcatcacc ggcgactcct
     8461 ccctccagaa tgccctcgat gtaatccggc tccccctgcg ccttcagata gtccaccaca
     8521 cgatgaactt cctcatcgga aacgaatgcg ccatgaacgc ggatcggcag gccagtaccc
     8581 ggcggcatat aaagcatgtc gcccataccg agcagcgctt ccgcgcccat ctggtcgaga
     8641 atcgtgcgcg agtcgactct gctgctgacc tgaaaggcga tgcgcgtcgg cacgttggcc
     8701 ttgatcaggc cggtgatgac atcgaccgaa ggacgctgcg ttgccaggat cagatggatg
     8761 ccagcagcac gcgccttttg cgcgatacgg gcgatcagct cctccacttt cttgccgacc
     8821 accatcatca gatcggccag ttcgtcgatg atgatgacaa tggtttcgag ctgttccagc
     8881 ggctccggcg catccggcgt caggctgaac ggattcggaa tcttttcacc gcgcgtttcg
     8941 gcatcggtga ttttctggtt gtagccgctc aggttacgca cacccagttt ggacatgcgc
     9001 ttatagcggc gctccatctc agccacggcc cagttcagcg catggccggc ctgacgcatg
     9061 tcggtcacga ccggcgccaa cagatgcgga atgccttcgt aaatcgatag ctccagcatc
     9121 ttgggatcga tcaagatcaa acgcacctgc ctggacgtag acttgtacag cagcgacagg
     9181 atcgtggcgt tgatgccgac cgacttaccg gaaccagtcg tgccggcaac cagtaaatgc
     9241 ggcatcctgg cgaggtcagc caccaccgga ttaccggcga tatccttgcc gagcgccaca
     9301 gtcaggttgg aacggctgtc gttataaacc ttggagtcga gtatctcggt cagacgcacg
     9361 atctggcgct tggtgttcgg cagttccaga cccatgtaat tcttgccttg aatcacttcc
     9421 accacgcgaa tcgaggtcag cgacagcgaa cgcgcaaggt cgcgcgccag accgacaatc
     9481 tggctaccct tgacgccagt ggcaggctcg attgcgtaac gcgtaatcac cgggccggga
     9541 taggcagcaa ccaccttcac tgtgacgccg aagtcggaca gtttcttttc gatcaggcgg
     9601 ctggtgaatt ccagggtttc aaccgacatc gtttgctgcg ccggaggcgc atcatccagc
     9661 aaggacagcg gcggcaggct ggaatccatg ccctcgaaca gactggtctg gcgctccttc
     9721 ccggtacgcc ccgacttcgg caccgccacc acctgcggct cgatatggat cggctgcgct
     9781 tcgacgatcc tggcgcgctc ctgcaccacc gcttcttcgc gcctgacggc ggcgacgcga
     9841 ccaaccttgc ggtcctgacg cgccagataa cgactcctta cccagcagat accgtcctcg
     9901 atgaaaccgc cgatgcgctc cgcaatctcc agccacgata cgtggaaaaa caggctgacg
     9961 cccaggccga acagcaacag taacagcaaa gtggcgccgg taaaaccgag cgacga
//
LOCUS       DASRDH010000082         3579 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_533127,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000082 DASRDH010000000
VERSION     DASRDH010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 3579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3579
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_533127"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..678)
                     /locus_tag="VFA14_02040"
     CDS             complement(<1..678)
                     /locus_tag="VFA14_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016836072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HZS80197.1"
                     /translation="MTRILRYLLVVGAAAAGILLFLLASASENSALFDQHYPWLLGLN
                     VLATISLLLLLLLLLTRLYKHYRRGKFGSKLLVRLVMMFALTGILPGAAIYMVSLQFV
                     SRSIESWFDVRIEAALEAGLNLGRNALDASLADLTARGRVMAQELADMSEVGQIGYLS
                     RQRDHNMEITLLNASGQVLATVGGRVGVLTPTVPTAAIMHQARLTRGFAIVESDDSRQ
                     LGGEPNPD"
     gene            complement(675..1280)
                     /locus_tag="VFA14_02045"
     CDS             complement(675..1280)
                     /locus_tag="VFA14_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007884413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HZS80198.1"
                     /translation="MQSAFILLHLTSFQCVRWWLRSVLLAFFLLFFCHTDMQAAEIQL
                     TQASLEATGNGYRLSTAYSFELNHSLEDALMRSVPLYFTTDVRLTRQRWYWFDEISVS
                     VSRTVRISFNVLTRQYHASTSDQLQQNFSSLEDVLALIRRPPRWIIADENALKSGDVY
                     KVSLRLRLDVTQLPKPFQIDALNNSDWRLSSDWKEFTFKVE"
     gene            complement(1285..2616)
                     /gene="rsmB"
                     /locus_tag="VFA14_02050"
     CDS             complement(1285..2616)
                     /gene="rsmB"
                     /locus_tag="VFA14_02050"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007884412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HZS80199.1"
                     /translation="MNVTQISLPSDSLAYGLHGAAQAITSVRAGTALPQALACLFTQN
                     GMTPQARGAIQDLSYLSMRRLGVADSLLNLLTDKPPQPRMLRSLLTCALSLLVEEQDP
                     AYPVFTVVNQTVEAAAADSRIRHAKGVVNAILRRFLRERVTLLAQAQKNPPGAWNYPA
                     WWIDRLKNVYPEQWQAILRAGNMPPPLTLRVNRRKTSVPAYVQALAVQGIDTCQVGSD
                     AVRLDRAMPVARIPGFAGGLVSVQDAAAQLAAPLLDVQDGMRVLDACAAPGGKTGHLL
                     ECADIDLLALDHDPRRLRRIEENLQRLQLRATLQTGDARYGANGLTDQRWWDGKPFDR
                     ILADVPCTASGIVRRHPDIRWLRRQTDTAQLAVLSAQILDNLWQMLKPQGKLLLVTCS
                     LWPQESEDQATAFVQRNQAIRLPAPGQLLPTACMETDHDGLFYALFQKTGP"
     gene            complement(2758..3096)
                     /locus_tag="VFA14_02055"
     CDS             complement(2758..3096)
                     /locus_tag="VFA14_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008334039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1840 domain-containing protein"
                     /protein_id="HZS80200.1"
                     /translation="MLVTFKSKAAADVLMYAAHAKPILDLLHKDIERGIITAAESGAA
                     IALLEEQIDLSKAHEAALEHDDHAHQHGDGDDAAHDKTGVVTFSARVYPLLDMLRRAR
                     KGNCDILWGV"
     gene            3325..>3579
                     /locus_tag="VFA14_02060"
     CDS             3325..>3579
                     /locus_tag="VFA14_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HZS80201.1"
                     /translation="MCVLLAPVFFPAHVFAQVAQSSDSEELRRRAQQEAMERQRQRQQ
                     ASVVLPRQAPTEDAFSYDLPVEELCFRIERLTLEIPAQTSD"
BASE COUNT          786 a         1015 c         1054 g          724 t
ORIGIN      
        1 gtccggattc ggttcgccgc ccagttgcct ggagtcgtcg ctttccacga tggcgaagcc
       61 acgtgtgagt ctggcctgat gcattatcgc tgcggtcggc acggtaggtg tcaggacgcc
      121 gacgcgtcca cccaccgtcg ccagcacctg gccgctggca ttgagcaggg tgatttccat
      181 gttgtgatcg cgttgccggg acagatagcc gatctgtccc acctcagaca tgtcggccag
      241 ttcctgcgcc atcacgcggc cgcgcgcggt gagatcggcc agcgatgcat ccagggcatt
      301 gcggccaaga ttgagaccgg cttccagcgc ggcttctata cgcacgtcga accacgactc
      361 gatcgaacgg gagacgaact gcagcgacac catataaatc gccgcgccgg gcaagatgcc
      421 ggttagcgca aacatcatca ccagtcgcac cagcagtttg gaaccaaact tgccgcgccg
      481 atagtgtttg tacagtcgtg tcagcagcag cagcagcagc agcagcaagg agatcgtcgc
      541 cagcacgttc agaccgagca accatggata gtgctggtcg aacagcgcgg agttttcaga
      601 cgcggaggcc agcaggaaca gcaggatgcc ggcggcggcg gcgcccacca ccagcagata
      661 gcgcaagata cgtgtcattc gaccttgaag gtaaattctt tccagtcgga agacaggcgc
      721 cagtcgctgt tattgagcgc gtcgatctgg aaaggtttag gcaattgcgt cacatccagg
      781 cgcaagcgca ggctgacctt gtagacgtcg ccggacttga gtgcgttctc gtcggcaatg
      841 atccagcgcg gtggccggcg gatcaacgcc agaacgtctt ccagcgagga aaaattctgt
      901 tgtagctggt cgctggtcga ggcatggtat tgccgtgtca gcacattgaa agaaatgcgc
      961 actgtacgcg acacggagac cgaaatttca tcaaaccagt accagcgctg gcgcgtaagc
     1021 cggacatcgg tagtgaagta cagtggtacg ctgcgcatca gcgcatcttc caggctgtgg
     1081 ttgagttcga aggaataagc ggtggacaga cggtaaccat tgccggtagc ctccagtgaa
     1141 gcctgggtaa gctggatttc tgccgcctgc atgtcggtat ggcagaaaaa cagcaggaaa
     1201 aacgccagca ggacgctgcg cagccaccaa cgcacacact gaaatgacgt cagatgcaag
     1261 aggatgaatg cagattgcat cgattcatgg tcctgttttt tggaacagcg cataaaacag
     1321 gccgtcatgg tcggtttcca tacaggccgt cggcagcaac tggcctggag ccggtaaccg
     1381 gatggcctgg ttgcgttgga caaacgcggt cgcctggtct tccgattcct gcggccacaa
     1441 cgaacatgtc accagcagta atttaccttg cggcttcagc atttgccaaa gattatccag
     1501 gatttgcgcg gaaagcacag cgagttgcgc cgtatcggtt tggcgacgca accagcgaat
     1561 atcaggatgg cggcgcacga tacccgaggc ggtacaaggg acgtcggcga ggatacgatc
     1621 gaatggtttt ccatcccacc agcgctgatc agtcaaacca ttggcaccgt agcgggcatc
     1681 accggtttgc agggtggccc tcagttgcag gcgttgcaga ttttcttcga tgcggcgtag
     1741 gcgtcgaggg tcgtggtcca gcgccagcag atcgatgtcg gcgcattcca gcagatggcc
     1801 ggtcttgccg cccggcgcag cgcaggcgtc gagtacgcgc ataccgtcct gcacgtccag
     1861 cagaggcgcc gccagctgag cggcagcatc ctgcaccgac accagccccc cggcgaagcc
     1921 gggaatgcgc gcgaccggca tcgctctgtc caaacgcacc gcatcagatc ccacctgaca
     1981 cgtatcgatg ccttgcacgg ccagcgcctg cacatacgcc ggcaccgaag tcttgcggcg
     2041 attgacgcgc agggtcagcg gcggcggcat attgcctgcg cgcagaatgg cttgccattg
     2101 ttcgggataa acgtttttga ggcgatcgat ccaccaggcc ggataattcc aggcgccagg
     2161 cgggtttttt tgcgcctgcg ccagcaacgt cacacgttca cgcagaaagc gacgcaggat
     2221 ggcattgacc acacccttgg catggcgtat ccggctatcg gcagcggcgg cttcgaccgt
     2281 ctgattgacg acggtgaaaa cagggtaagc cggatcttgc tcttccacca gcaggctgag
     2341 cgcacaagtc aacagactgc gcagcatcct cggttgcggc ggtttgtcgg tgagcaggtt
     2401 cagcagactg tcggccacgc ccagtctgcg catgctcaaa taagataaat cctggatagc
     2461 gccgcgcgcc tgcggcgtca tgccgttttg ggtaaagagg caggccagcg cctgcggcag
     2521 ggcagtgccg gcgcgtacgc tggtgatggc ctgtgcagcg ccatgcaggc cgtaggccaa
     2581 agagtctgat ggcaaggaaa tttgagtcac attcatgcct gcataaggcc gggtttacgt
     2641 cgaggtcgtt caggctgtag ggaatgtcag tgtaccagcc acgcaggtct tgccggccat
     2701 ttctgcgcag cggctgaaaa acgatacaag aacacagctc ctctgtgacg atgcgactca
     2761 gacgccccaa aggatgtcgc agttgccctt ccttgctcga cgcagcatgt ccagcagcgg
     2821 ataaacacgc gccgaaaacg tcaccacccc ggttttgtca tgcgcggcat catcgccgtc
     2881 gccatgctga tgcgcatggt catcgtgttc cagtgctgct tcgtgcgctt tgctcaagtc
     2941 gatctgttct tccagcaggg cgatggcggc gccgctttca gcggcggtga tgatgccgcg
     3001 ctcgatgtct ttgtgcagca ggtcgaggat aggtttggca tgcgctgcat acataaggac
     3061 atcagcagcg gcttttgatt tgaacgtgac cagcatattt tctcagggta tgtattcaag
     3121 cctccttttt attacatttt tagaaaaatt acgcgctgat aagagccaac ctcgaacaaa
     3181 aacaaaaata aatgcccaaa gatgcagctt taatattgct tattggcaat caaaatactt
     3241 acactccgct tttctcagtc agcaatttat cagcctgtga taacgataag aatgaatagc
     3301 cgcgtccatc ggggaatggc cggcatgtgt gtcctgcttg ctcctgtttt cttcccggct
     3361 catgttttcg cccaggtcgc gcagagcagc gactcggagg aactgcgtcg tcgtgcgcaa
     3421 caggaagcca tggagcggca acgacaacgg cagcaagcat ccgtggtatt gcctcgacag
     3481 gcaccgactg aagatgcttt ttcctatgat ctccccgtcg aggaattatg tttccggatc
     3541 gagcgtctga ctctggaaat acccgcccag accagcgat
//
LOCUS       DASRDH010000083         3288 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1184570,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000083 DASRDH010000000
VERSION     DASRDH010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 3288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3288
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1184570"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="VFA14_02065"
     CDS             <1..405
                     /locus_tag="VFA14_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HZS80202.1"
                     /translation="DRSVRALQTWELLHQDDGFGRAELLWRLALPLMALTLMLLAIPL
                     SFVNPRAGRSVGLLMAVLLFVLYSNTVSIFQAAVMQNRLSFMLGWWPVHLAMVLLVFA
                     MFFLRLNVNSRYHPAVIWSCVRRAFLRKRQAR"
     gene            402..1532
                     /gene="lptG"
                     /locus_tag="VFA14_02070"
     CDS             402..1532
                     /gene="lptG"
                     /locus_tag="VFA14_02070"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HZS80203.1"
                     /translation="MSVLQRYIAVGVVRSVSFALAAFLALFAFFELMGQLESVGHHGY
                     QLQTALLYVAMGLPGNVYELMPIAVLIGTIYALAQFAATSEFTIMRVSGMSTRMTVKA
                     LARIGIGFAIVTMLFGELLAPKASEWGEKLRMQAQGASLSSQFRSGLWAKDVIRENGL
                     KGKVIGTRFLNIQRMSSDGRLDGVRIYELDQDFHLSELIVAARGEYQGDHVWLLMDVQ
                     QTTFINSNTSDIAAPIATGRVKARSFVSEVTPEILSVLLTNPERMSAYDLLTYTRHLE
                     ANNQRAERYEIAFWKKIVYPLSVFVMMALALPFAYLHFRAGGVSLKIFTGIMIGVSFQ
                     LISNLFAHLGLLNAWPAFFSVALPSMLFMLLAMIALRWVERR"
     gene            1543..1914
                     /locus_tag="VFA14_02075"
     CDS             1543..1914
                     /locus_tag="VFA14_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006712773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HZS80204.1"
                     /translation="MKKSLILFVHGARDPDWAKPFHRLQTLTQASLPDVVVELAFLEL
                     MTPNLLQLAARQVADGVRHITVVPVFLGQGGHVRHDLPVLVEQLRRTHPQVTFQVAEA
                     AGEDAAVLQALARYCITRLGS"
     gene            2520..>3288
                     /locus_tag="VFA14_02080"
     CDS             2520..>3288
                     /locus_tag="VFA14_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS80205.1"
                     /translation="MAAEKDTGKSSELTVDIIIGGLGKTQEPILSAAQKALLESFKVA
                     ANLEDLAARLVIPIQLATVTGAKVISAHGYDAYKITIAVKSDDPNKVAREIFTTAAGL
                     TAAPVGVVAGKLIVHSFPSGILYSSIAGAAGGVVGGVIASELAAFLWDNTLQNTAAGQ
                     WSIGQLTDSFGIGLKITQGSPGNTASHADMPPANQTPDTALVFDPASNQAGIVFRDRT
                     RPATGNSKLYDVTHVTLTHGPLAGQTLSITTNTQTGEI"
BASE COUNT          660 a          867 c          961 g          800 t
ORIGIN      
        1 gacagatcgg tgcgcgcgtt gcagacctgg gagctgctgc accaggatga tggttttggc
       61 cgcgctgaac tgctctggcg tctggcgctg ccgttgatgg cgctgacgct gatgctgctg
      121 gcgatcccgc tgtctttcgt caatccgcgc gcggggcgct cggtcggcct gctgatggcc
      181 gtgctgctgt tcgtcctgta cagcaatacg gtcagcatat tccaggcggc ggtgatgcag
      241 aaccgtctat ccttcatgct cggctggtgg ccggtgcatc tggcaatggt gttgctggtg
      301 ttcgccatgt tttttctgcg gctcaatgtc aatagccgct atcaccctgc agtcatatgg
      361 tcttgcgtgc gacgcgcgtt tttacggaaa cggcaggctc gatgagcgtc ctgcaacgtt
      421 atatcgctgt cggggttgtg cgctcggtct ctttcgcgct ggcggccttt ctggcgctgt
      481 tcgccttttt tgagttgatg ggccagctgg agtctgtcgg ccatcatggc tatcagttgc
      541 agactgcctt gctgtatgtg gcgatgggtt tgcccggtaa cgtgtatgaa ctgatgccga
      601 tcgcggtatt gatcggtacg atttacgcgc tggcgcagtt cgccgccacg tcggaattca
      661 ccatcatgcg cgtatcaggt atgtccacgc gcatgaccgt caaggcgctg gcgcggattg
      721 gcatcggctt tgccatcgtc acgatgttgt tcggcgagct gctggcgccg aaggccagcg
      781 aatggggtga gaaactcagg atgcaggcgc agggcgcgtc gctgtcctcg caattccgtt
      841 caggcctgtg ggccaaggat gtgatcaggg agaatggctt gaaaggcaaa gtgatcggca
      901 cgcgtttttt gaatatccag cgcatgtcga gcgatggtcg tctcgacggc gtcaggattt
      961 atgagctgga tcaggatttt catctgtcgg agctgatcgt cgcggcgcgg ggggagtatc
     1021 agggcgatca cgtatggctg ctgatggatg tgcagcagac gactttcatc aacagtaaca
     1081 ccagcgacat cgctgcgccc attgcgaccg gcagagtcaa ggcgcgcagc ttcgtctcgg
     1141 aagtgacgcc ggaaatcctg tcggtgttat tgaccaatcc tgagcgcatg tccgcctatg
     1201 acctgctcac ttacaccagg catctggaag ccaacaatca gcgcgccgag cgctacgaga
     1261 tcgctttctg gaaaaagatc gtctatccgc tttccgtttt cgtcatgatg gcgttggcct
     1321 tgccgttcgc gtatctgcat ttccgtgctg gcggcgtcag tttgaagatt tttaccggca
     1381 tcatgatcgg cgtcagtttt caattgatca gcaatctgtt cgcgcatctg ggtttgctca
     1441 acgcctggcc ggcgtttttt tcggtggcgt tgccgagcat gttgttcatg ctgttggcga
     1501 tgatcgcctt gcgctgggtc gagcggcggt gaggcaaagg cgatgaaaaa atcattgatc
     1561 ctgttcgttc acggtgctcg tgatcctgac tgggcgaaac cgttccatcg cttgcagaca
     1621 ctgacgcagg caagtctgcc ggatgtcgtc gtggaactgg cttttctgga actgatgacg
     1681 ccgaacctgc tgcaattggc ggcgcggcag gtcgctgatg gcgtgcggca tatcacggtg
     1741 gtgccggtgt ttttggggca gggggggcat gtccggcatg atttgccggt cctggtcgag
     1801 cagttgcgcc ggacccatcc gcaggtcaca tttcaggtcg ctgaagcagc cggcgaggat
     1861 gccgctgtct tgcaggcgct ggcgcgatat tgcatcacca ggctgggatc gtgagccggc
     1921 tctaactgtc agttttaaat cgctggttcc taaaagaatc tgatccaggc cgccgaaatg
     1981 ctgacgccaa ttttttttgt tcagcctgtt actgatggcg ggttttggtg gcagcttcga
     2041 tcatatgagc gagcagattg gcggtgtcaa cggacgtatt tcatgactct atcgcagatt
     2101 aattgacaca ccattccact cacttcgaga agatcttttg ttgccagtga accaatactg
     2161 caagcaaaaa tctacttctt ccatgtgcga cattaccgtc acacactgaa agaaatatct
     2221 ctcgacacgt ctgcatctcc attttttacg attcaaaaac gattccctca tcgggcaaag
     2281 gagaatgatc ctggctattg aaaaaaatgt ctgcggcaat cgggagtttc gagagtctgg
     2341 aatgcggaat tggataaaac aagacggtga tggctggaaa tcaagaagaa aagcattttt
     2401 cgccggactc gtgggggggg gggtatattt ccgttcatgt ggtacttctt gatttttcat
     2461 tcgtttttgc tgtttgcatt taaatctcac gtgatttcat ccagtaaagg agaataatct
     2521 tggctgctga aaaagacaca ggcaaatcat ccgagttgac ggttgatatc atcatcggcg
     2581 gtctgggaaa aacccaggaa ccgatattgt ccgcggcgca gaaagccctg ctggaatcat
     2641 tcaaggtcgc agcgaatctg gaagacctgg cggcgcgtct cgtcattcca attcagcttg
     2701 cgaccgtcac gggagccaag gtcatatcgg cgcatggata tgatgcctac aagatcacca
     2761 ttgcggtcaa gagcgacgat ccgaacaagg tggcgagaga aatcttcacg acagcagctg
     2821 ggttgactgc ggcacctgtt ggcgttgtgg cgggtaaact aatagtccat tcgttcccaa
     2881 gtggaatact ttactcgagc atcgcgggag cagctggagg ggtagtagga ggtgttattg
     2941 cctcagagct tgccgctttc ctctgggaca acactctcca aaacaccgcc gccggccaat
     3001 ggtcgatcgg tcaactgacg gattcattcg gcatcggcct gaagatcacc cagggcagcc
     3061 ccggcaatac cgcttcccat gccgatatgc ctcccgccaa ccagacgccg gataccgcgc
     3121 tggtattcga tccggccagc aatcaggccg gcatcgtctt ccgcgacagg acgcgcccgg
     3181 ccaccggcaa cagcaagctc tacgacgtca cccacgtgac cctcacccac ggccctctgg
     3241 ccggacagac cctctccatc accaccaaca cgcagaccgg cgaaatca
//
LOCUS       DASRDH010000084         2693 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1294150,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000084 DASRDH010000000
VERSION     DASRDH010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1294150"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="VFA14_02085"
     CDS             complement(<1..350)
                     /locus_tag="VFA14_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HZS80206.1"
                     /translation="MQLDNRAQTLLKTLIERYIADGQPVGSRALSKISGLELSPATIR
                     NIMSDLEDMGFVASPHTSAGRIPTPRGYRVFVDTLLTVESIDEAALENRLQSHPTLNS
                     PHKIIANTAQVLSSL"
     gene            478..1395
                     /locus_tag="VFA14_02090"
     CDS             478..1395
                     /locus_tag="VFA14_02090"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006461796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0019674 - NAD metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HZS80207.1"
                     /translation="MWPIKLPVRTMTPKTIEIVGKFHAAGITQSLSDIAAFLKARGYT
                     VVFEAETARNIALEGYDAMSPEQIGRFADAAIVVGGDGTMLGIARQLAPFDVPLIGIN
                     QGRLGFMTDISLDRMIPVLADMLDGKVEAEARTLLEATVSRDGVQIFHALALNDVVVS
                     RGAFSGMVELSVEVDGRFMYNQRSDGLIVATPTGSTAYALSAGGPILHPNLSGIVLAP
                     IAPHALSNRPIVIPDSSEIAIEVVNGRDISANFDMQSLTSLSHGDRIVIRRSASKITF
                     LHPEGWSYYGTLRQKLYWNEYPSMEGRLQ"
     gene            1479..>2693
                     /locus_tag="VFA14_02095"
     CDS             1479..>2693
                     /locus_tag="VFA14_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008325483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZS80208.1"
                     /translation="MLRTLSIRDFVIVDAIELEFAPGFSVFTGETGAGKSILIDALVL
                     ALGGRGDAGVVREGAARADISAGFSVGPEAAAWLDTHAFADEDGVLLRRVIDDAGRSK
                     AFINGIAATVTQLRALGELLVDIHGQHAHQSLLKVDVQRQLLDSQAGLLDEAKAVAAA
                     YETWRAAVRQREEFNVNASKVLLEREGLERQVEELEKLALKPGEWAEISNEHRRLSHA
                     AGLIDAAQEALSAISESESPLLSQLSLLTQKLGRLTDVDTALEPVMEALEQARIQLQE
                     AAYALNDYLGRVELDPARLQQVEGRLEAMYSMARKFRITPEDLPQEHEKLAQALRQLV
                     DAGDLDALRVQEEKWCAAYTSAAQTLSQGRAKAAQALSAAVTAAMQELSMVGGRFSIA
                     LTACDPAAYGMEQ"
BASE COUNT          534 a          756 c          825 g          578 t
ORIGIN      
        1 agcgaggaca gcacctgcgc ggtgttggca ataattttgt gcggcgaatt gagcgtcggg
       61 tgcgattgta accgattttc cagcgccgct tcgtcgatcg actcgacggt cagcagcgta
      121 tcgacgaaaa cgcgataacc gcgcggcgtc ggaatccgtc cggcggaagt gtgcggactg
      181 gcaacaaagc ccatgtcttc gagatccgac atgatgttac ggatggtggc cggtgacagt
      241 tccagtcccg aaatcttgga aagcgcgcgc gagccgactg gctgaccgtc agcgatatat
      301 cgttcgatca gggttttgag cagggtttga gcgcgattgt ctagttgcat gttctcattc
      361 taacgatcac acgaaaagcc cgccagtatc ttctcgatgt tttagtgtat ggcctatgta
      421 tctttttgtt catgcaaatc gattcagaat cgtccggatt gtgctttaat ccgcagcatg
      481 tggcctatca aattacccgt gcggacgatg acgcctaaaa ccattgaaat tgtcggcaaa
      541 tttcatgccg ccggtattac tcaatccctg tcggacattg ccgctttcct gaaagcgcgt
      601 ggctacactg ttgtctttga agccgagact gcgcgcaaca tcgcgctgga aggctatgat
      661 gccatgtcac cagagcagat cgggcggttc gccgatgccg ccatcgtggt cggcggcgac
      721 ggcaccatgc taggcatcgc gcgccagctg gcgcctttcg acgtgccgct gatcggcatc
      781 aatcaggggc gtctgggttt catgacggat atttctctgg atcgcatgat accggtattg
      841 gccgacatgt tggatggaaa agtggaagcc gaggcgcgta ccctgctgga ggcgacggtg
      901 tcgcgcgacg gcgtgcaaat tttccacgcg cttgcgctca acgacgtggt ggtatcgcgc
      961 ggtgcttttt ccggcatggt cgaactgagc gtcgaagtcg atggccgctt catgtacaat
     1021 cagcgttccg atggtctgat cgtggctacg ccgaccggtt cgaccgccta tgccctgtcg
     1081 gccggtggtc cgatcctgca cccgaacctg agcggtatcg tgctggcgcc gattgcgccg
     1141 catgccttgt cgaatcgccc gatcgtgatt cccgattcca gcgaaatcgc cattgaggtc
     1201 gtcaatggcc gtgacatcag cgccaatttc gacatgcagt cgctgaccag cctgtcgcat
     1261 ggcgaccgca tcgtcatccg gcgctcggcg agcaagatca cttttctgca tcccgaaggc
     1321 tggagttatt acggcactct gcggcaaaaa ttgtactgga acgaataccc ctcgatggaa
     1381 ggccgtctac aatagatagt cgccggcctg cgactgcggt cgtcctgcat gaatatagca
     1441 gcagccggta ttttcaatga aacagttcct gcacgtccat gctgcgtacc ctgtcgatcc
     1501 gtgatttcgt gattgtcgat gccatcgagc ttgagttcgc tcctgggttt tccgtcttca
     1561 ccggcgaaac tggcgcgggt aaatcgattc tgatcgatgc gctggtgctg gcgctcggcg
     1621 gacgtggtga tgccggcgtg gtgcgcgaag gcgcggccag ggccgacatc agtgccggtt
     1681 tcagcgtcgg tccggaagct gccgcctggc tcgacacgca cgccttcgcc gacgaagacg
     1741 gcgtgctgct gcgccgtgtg atcgatgatg ctggccgctc caaagccttc atcaacggca
     1801 tcgccgccac cgtcacgcaa ctgcgcgcgc tgggtgaatt actggtcgat atccatggcc
     1861 agcatgccca tcaatccttg ctcaaggtcg atgtccagcg tcagttgctg gatagccagg
     1921 ccggtctgct cgatgaagcg aaggcggtcg ccgctgccta cgaaacctgg cgcgcggcgg
     1981 tgcggcagcg cgaagaattc aatgtcaacg ccagcaaggt tttactggaa cgcgaaggtt
     2041 tggagcggca ggtcgaagag ctggaaaagc tggcccttaa gcccggcgaa tgggccgaga
     2101 tcagcaatga gcatcgccgt ctgtcgcatg ccgccggcct gatcgatgcc gcgcaggaag
     2161 cgctcagcgc gatttcagag tcggaatcac cgctcctgtc gcaactgtcg ttactgacgc
     2221 agaaactggg caggttgacc gacgtcgata ctgcgctcga gccggtcatg gaggcgctgg
     2281 agcaggcgcg gattcaattg caggaagcag cttacgcgct caatgattac ctcggccggg
     2341 tcgaactcga tccggcacgc ttgcagcaag tagaagggcg actggaagca atgtactcga
     2401 tggcgcgcaa attccgcatc acgccagagg acttgccgca agaacatgaa aagctggcgc
     2461 aggcgttgcg gcaactggtc gatgccggcg atctcgatgc cttgcgcgtt caggaagaaa
     2521 aatggtgcgc ggcctatacc agcgccgcgc aaaccttgtc acagggccgc gccaaagccg
     2581 cccaggcctt gagcgcagcg gtgacggcgg cgatgcagga actgagcatg gtcggtggcc
     2641 gtttttcgat tgcgctgaca gcttgtgatc cggctgctta tggtatggaa cag
//
LOCUS       DASRDH010000085         4269 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1182022,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000085 DASRDH010000000
VERSION     DASRDH010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 4269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4269
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1182022"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..804
                     /locus_tag="VFA14_02100"
     CDS             <1..804
                     /locus_tag="VFA14_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007880133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo hydroxylase family protein"
                     /protein_id="HZS80209.1"
                     /translation="VKGLEAGKLLYFPHLPFALLPEEESLLQPHIRNPKVRNISLDAR
                     GHLKGAAGDTATQLALASMIGRFREQARSLVHSLLPKYRSALRLAPTSYRPTQVETRA
                     QSWRADDRRLHVDAFPSRPNHGERILRVFCNINPDGVPRVWRVGEPFETVAGKFLPRA
                     KPYVPWQAKTLEALRVTKSLRSEYDHLMLQLHDGMKQDLEYQKNAGQVSMPFAAGSVW
                     VCFSDQTSHAVMSGQYMLEQTLHLPADGQYDPTASPLAILGRLTGRQLV"
     gene            801..2075
                     /gene="waaA"
                     /locus_tag="VFA14_02105"
     CDS             801..2075
                     /gene="waaA"
                     /locus_tag="VFA14_02105"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017454322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HZS80210.1"
                     /translation="MILLYSALWWVGLPVVLLRLWRRGSREPGYRQHIAERLGFYDQF
                     AHQPTPPIIWVHAVSVGETRAAQPLIEALLSAYPQHTVLLTHITATGRATGQQLFSSR
                     SRVIQSYFPYDTGWMCARFLRYFRPQICILMETEVWPNMLHQCRRHHIPVLLANARLS
                     ARSLRRGCRFGALLREAAASIDCVAAQSERDAKRLRLAGARRVHVTGNIKFDVAAPAS
                     MTALGRQWRMQSGARPVLLCASTREGEEELILHALSRIERRDFLTIIVPRHPQRFDAV
                     AAMIDARGFAMQRRSHGMQAGIATATDIVLGDSMGEMFAYYATCDVAFIGGSLLPLGG
                     QNPIEACLLGKPVLTGSHTFNFQVICDAAVAAQAALRVTDATQMLHQAQSLFHDHDRH
                     AAMSRHAAAFAQQHQGATAHTMALIASMLHAG"
     gene            complement(2199..3251)
                     /gene="queA"
                     /locus_tag="VFA14_02110"
     CDS             complement(2199..3251)
                     /gene="queA"
                     /locus_tag="VFA14_02110"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013233250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HZS80211.1"
                     /translation="MYSLSDFDFDLPPELIAQLPLAQRSASRLLQVDGATLTDRCFAG
                     VVDLLAPGDLLVFNDTRVLKARFSGIKETGGKIEVLVERVLGNGSVYAQVRASKSPRP
                     GARLRLADAFEVTVGERHGEFYTLQFPSGVLALLEQYGSLPLPPYIAHAADAFDEARY
                     QTIYAREPGAIAAPTAGLHFDAALLGSLQARGIATAFVTLHVGAGTFQPVRVENLAEH
                     SMHSEWYAITQDTVNKVRAAQAAGGKIVAVGTTSLRALESASQSGALEAGSADTRLFI
                     TPGYAFRTVDRLISNFHLPRSTLLMLVSAFAGYDHIRAAYLHAIAQGYRFFSYGDAML
                     LSRTGPDWEAPAFGME"
     gene            3367..>4269
                     /locus_tag="VFA14_02115"
     CDS             3367..>4269
                     /locus_tag="VFA14_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007877813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HZS80212.1"
                     /translation="MPAPKKKASAAPSTSNAQQAQNRLVRLGLRSDMDLALHLPLRYE
                     DETQIFRIHDASLHGTRVVQVEGIVTKSDIQSRPRRQLVVTIADDSGALALRFLNFYG
                     SQARQLAEGVRVRARGELRHGFCGAEIVHPTYKIVLEGAPLPTALTPVYPAGDGLPQA
                     YLRKAIAVALTRIDWRDTLPQATLDELQLASFASSVRLLHNPPADVDAYALEKRSHPA
                     WIRMKFDELLAQQLSMRKHYRERKARRAPVLEVPIEKARRYTSALIGRLPFQLTRAQQ
                     RTWGEIEGDLARPHPMHRLLQGDVG"
BASE COUNT          859 a         1340 c         1261 g          809 t
ORIGIN      
        1 gtcaaggggc tggaagccgg caagctgctg tattttccgc atctgccgtt cgcgctgttg
       61 ccggaggaag aaagcttgct ccagccgcac atccgcaatc cgaaagtccg caacatcagt
      121 ctggatgcgc gtggtcatct gaaaggcgcc gccggcgata ccgccaccca gttggcgctg
      181 gcatcgatga tcgggcgctt tcgtgagcaa gcccggtcgc tggtgcattc cctgctgccg
      241 aaataccgta gcgcgttgcg gctggcgccg accagttatc gtccaaccca ggttgaaacc
      301 cgcgcgcaat cctggcgcgc cgatgaccgg cgcctgcacg tcgacgcctt tccatcgcgg
      361 ccgaatcatg gcgagcgcat cctgcgcgtg ttctgcaaca tcaacccgga tggcgtgccg
      421 cgcgtctggc gcgtcggcga accattcgag accgtcgccg ggaaattcct gccacgcgcc
      481 aaaccgtatg taccgtggca ggccaagacg ctcgaagcgc tgcgcgtcac gaaatccttg
      541 cgcagtgaat acgatcatct gatgctgcaa ctgcacgacg gcatgaagca ggatctggaa
      601 taccagaaaa acgctgggca ggtaagcatg ccgtttgccg ccggctccgt ctgggtgtgc
      661 ttctcggatc aaacctcgca tgcggtgatg tccgggcaat acatgctgga gcagacattg
      721 catctgccgg ccgatgggca atatgacccc accgccagtc cgctcgccat tctgggcaga
      781 ctcaccgggc gccaactggt ttgattctgc tgtattccgc actatggtgg gtcggcctgc
      841 ctgttgtgct gctgcgcttg tggcggcgcg gcagcaggga gccgggttat cggcaacata
      901 tcgcggaacg tctgggtttt tatgatcaat tcgcccatca accaacgccg ccgatcatct
      961 gggtgcatgc ggtttccgtc ggcgaaacgc gcgcagcgca gccgctgatc gaggctttgc
     1021 tatcggcgta tccgcagcac accgttctgt tgacacacat aaccgctacc gggcgcgcga
     1081 cgggacaaca gttattctcc tcccgtagcc gtgtgatcca gagttatttt ccttacgata
     1141 ccggctggat gtgcgcacgc tttttgcgct atttcaggcc gcagatctgc atcctgatgg
     1201 aaaccgaagt gtggccgaac atgctgcatc aatgcagacg ccaccatatc ccagtgctgc
     1261 tcgccaatgc gcgcctgtcg gcgcgctcgc tacggcgcgg ctgccgcttt ggcgcactcc
     1321 tgcgggaagc cgccgccagc atcgattgcg tcgcggcgca atcggaacgg gacgccaaaa
     1381 gattgcgtct ggccggcgcg cgccgggtgc atgtgaccgg caatatcaag ttcgacgtcg
     1441 cagcacccgc cagcatgacg gcattgggaa gacaatggcg catgcagtct ggagcgcgtc
     1501 cggtgctgct gtgcgccagt acgcgcgaag gtgaggaaga actgattctg catgccttga
     1561 gccggatcga gcgcagagat tttctgacca tcatcgtgcc gcgccatccg cagcgtttcg
     1621 atgcagtggc ggccatgatc gacgcgcgcg ggtttgccat gcagcgtcgt tcgcatggca
     1681 tgcaagccgg catcgcaacg gcaacggaca tcgtgttagg tgattcgatg ggggaaatgt
     1741 tcgcctatta tgcgacgtgc gatgtcgcgt tcataggcgg cagtctgtta ccgctgggag
     1801 gacagaatcc gatcgaagca tgcctgctcg gcaagccggt gctgacagga tcgcatacct
     1861 tcaattttca ggtgatctgt gatgcagcgg tcgcggcaca ggccgcgctg cgcgttacag
     1921 atgccacgca gatgctgcat caggcacagt cgctgtttca cgatcacgac cgtcacgccg
     1981 ccatgtcacg ccatgccgcc gctttcgcgc agcagcacca gggcgccacc gcgcatacga
     2041 tggcgctgat agcctccatg ctgcatgcag gatgaggctc ctcctgtttt tcgccttcca
     2101 gcgttcccgg gtgatgcagc cacatccttg atctgcccga gccttgatcg agtcttgcat
     2161 gaactggaat tatcttgcta tctactcgct ccgacacatc actccatccc aaaggcgggt
     2221 gcctcccaat caggccctgt cctggacaaa agcatggcat cgccatagct gaaaaaacga
     2281 tacccttgcg caatggcgtg caggtaagcg gcgcgaatat gatcgtaacc ggcaaacgcc
     2341 gaaaccagca tcagcagtgt cgatctgggc aagtggaagt tgctgatcag gcgatccacg
     2401 gtcctgaacg cataacccgg cgtgatgaac agccgcgtgt cggcgctacc ggcttccagc
     2461 gcgcccgatt gtgaggcaga ctccagcgcg cgcaggctgg tcgtgccgac agcgacgatt
     2521 ttgccgcccg ccgcctgcgc tgcacgtacc ttgttcacgg tatcctgcgt gatggcgtac
     2581 cactcactgt gcatgctgtg ctcggccagg ttctccacac gcaccggctg aaaggtgcca
     2641 gcgccgacgt gcagcgtgac gaaagcggtg gctatgcccc tggcctgcaa gctgccgagc
     2701 aaggctgcgt cgaaatgcag acctgccgtc ggcgcggcga tggcgcccgg ttctctggca
     2761 tagatggtct gataacgcgc ctcgtcgaat gcatcggctg catgcgcgat atacggcggc
     2821 aatggcaggc tgccgtattg ctccagcaat gccagcacac cagagggaaa ctgcaatgtg
     2881 taaaattcgc catgacgctc gccgacggtc acctcaaaag catccgccaa acgcaggcgt
     2941 gcgcctggcc gcggtgattt ggaagcgcgc acctgcgcat acacgctacc gttgccgagt
     3001 acgcgctcga ccagcacttc gatcttgccc ccggtttcct tgatgccaga aaaacgcgcc
     3061 ttcagcacgc gcgtgtcgtt gaacaccagc aaatcgcccg gcgccagcaa atcgaccaca
     3121 ccggcaaagc agcgatcggt cagcgtcgcg ccatcgacct gcaacagacg cgaggcgctg
     3181 cgctgcgcca gcggcaactg tgcaatcaat tctggcggca agtcgaaatc aaaatcggaa
     3241 agtgaataca tgagaatcag caagagcggc gaaaatactg taattaaaca cagtaaaata
     3301 agaacgtaaa cacgttctca ggccaaacca gccattttac gcttgctcga cataaagacc
     3361 ctctgcatgc cagcaccgaa aaagaaagct tctgccgccc catcgaccag caatgctcaa
     3421 caggcgcaaa acaggctcgt caggctgggc ctgcgctcgg acatggatct ggccttgcat
     3481 ctgccgctgc gctacgagga tgagacgcag atcttccgca tccacgatgc cagcctgcac
     3541 ggcacccggg tcgtgcaggt cgaaggcatc gtcaccaaaa gcgacataca gtcccggcca
     3601 cgccgccagc tggtcgtgac gattgctgat gacagcggcg cgctggcgct gcgctttctc
     3661 aacttctacg gcagtcaggc caggcaattg gccgaaggcg tgcgcgtgcg cgcacgcggt
     3721 gaattgcgtc acggcttctg cggcgccgag atagtccatc cgacctataa gatcgtactc
     3781 gaaggcgcac cgttaccgac tgcgctgacg ccggtctatc cggctggcga tggcctgccg
     3841 caagcctatc tgcgcaaagc catcgccgtt gcgctgacgc gtatcgactg gcgtgacacc
     3901 ttgccgcagg caacgctgga cgaactgcaa ctggcttcct tcgcgtcttc ggtacgcctg
     3961 ttgcacaatc cgccggctga cgtcgatgca tacgcgctcg aaaaacgctc gcatccagcc
     4021 tggattcgca tgaaattcga tgagctgctg gcgcagcagc tctccatgcg caagcactac
     4081 cgtgagcgca aggcgcgcag ggctccggtg ctcgaggtgc cgatcgaaaa ggcgcgtcgc
     4141 tatacgtctg ccctgatcgg gcggttgccg ttccagctga cgcgagccca gcagcggacc
     4201 tggggagaga tagagggcga tctcgcccgg cctcatccca tgcatcgcct gctgcagggc
     4261 gacgtcggc
//
LOCUS       DASRDH010000086         7232 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1165924,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000086 DASRDH010000000
VERSION     DASRDH010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 7232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7232
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1165924"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            609..1910
                     /locus_tag="VFA14_02120"
     CDS             609..1910
                     /locus_tag="VFA14_02120"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF012372.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HZS80213.1"
                     /translation="MNSEHLVLSLFSGAGGFSYGFSQAGLKPLFGAEINEDACQSYEL
                     NIQSPCHAVDLSTVNPSLFKAMTGKRTPFAIIGGPPCQGFSTAGPRNANDPRNRLIFN
                     YLAIVEELKPRWFIFENVEGLLTSGGGQDVARLVKEFIGLGYSVRLQKVNLAAFGVPQ
                     TRKRVIIIGNRLGIDFEFPEEIYSYDSGKVKKLSNKPMAPTLDEAIAGLGSPVLDRSI
                     SPYLPNEPVNAFDAAMRLENGGEGVTQHIQCSDSVEKIHLELLKPGQTMKDLPSQYWH
                     ESFERRANRRVSDGTPTEKRGGAPSGIKRLHGNLQSLTITGAASREFIHPRENRPLTI
                     RECARIQTFPDHYKWKGNAASVIQQIGNAVPPLAASVLARHIQHVDGQFGSDIQASHK
                     DGKPRLLGFVLTEASGMSPALKATQSLLRNVHQENLELNES"
     gene            1900..3030
                     /locus_tag="VFA14_02125"
     CDS             1900..3030
                     /locus_tag="VFA14_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS80214.1"
                     /translation="MKAKTKRLSAEKSQQLTRLITITKTATMKAVMDPSELAKVITLV
                     ATDIGKGDEMAEAFPNIWEGIAPPSDYYSLTLDWFTNQIGGVSAFDVVDMLDHGETLD
                     EDFLAYLKCLMELHKRRRKYGLILQRQPLPTMVQVSPRALMEYGPDFQPEALASWLTW
                     RKFFYDLDNRSAQETGYLFEPILAAAIGGEPKSARECVVRRSDDQAKGRQVDCWKVLP
                     DGTALAYELKLRVTIAASGQGRFGEELSFAQDCRGSGVKPVLVVLDPTENGKLTGLQV
                     AYCAAGGDAYVGDAAWAHLEDEAGPTMAAFIERYVRTPVNAVTAFETSLEGEPSRRSI
                     ALLDLAAKLEGNDLTITLGQHQRKIERHEDPLLSDEDDSPEE"
     gene            complement(3114..3180)
                     /locus_tag="VFA14_02130"
     tRNA            complement(3114..3180)
                     /locus_tag="VFA14_02130"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3145..3147),aa:Thr,seq:cgt)
     gene            complement(3499..5811)
                     /locus_tag="VFA14_02135"
     CDS             complement(3499..5811)
                     /locus_tag="VFA14_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016833302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HZS80215.1"
                     /translation="MDSTNSKKEEIRQQLRQAALEYHEFPAPGKISVTPTKLLTNQRD
                     LSLAYSPGVAAACEEIIADPANAFRYTARGNLVAVISNGTAVLGLGNIGPLASKPVME
                     GKGVLFKKFAGIDVFDIEINETDPDRLCDIIAALEPTFGGINLEDIKAPECFYIERKL
                     RDRMKIPVFHDDQHGTAIIVGSAILNGLKVVGKDIREVKLVVSGAGAAALACLDLIID
                     LGFPIQNIYVTDLAGVVYKGRTELMDPDKERFARETDARTLADVIPDADIFLGVSAGG
                     VLKQDMVKKMAPRPLVLALANPTPEILPEDVKAVRDDAVIATGRSDYPNQVNNVLCFP
                     YIFRGALDSGATTITREMEIAAVHAIAELAQVEQSDVVTATYGMPHLSFGPEYIIPKP
                     FDPRLMTKIATAVARAAEASGVAARPIRDMDAYADKLEQCVYHSGTLMRPVFQAAKKA
                     PDSKKRIVYAEGEEERVLRAVQVVVDEGLARPILIGRPEVLEQRIRKFCLRLRPGADF
                     EVINPNFDNRYRDYWQTFLAMTKRKGVTEQYARLEMRRRHTLIGAMAIYKGDADGMIC
                     GTYGTTQLHVHYIDQVLGKRAGVNVYAAMNCVILPNRQLVLVDTHVNENPTAAELAEI
                     TILAAEEMRRFGLHPRAALLSHSNFGSSNSESACKMRAALALIQQQAPDLEVDGEMHG
                     DTALDAKLLAQLMPDSPLKGDANLLVMPNIDAANIVYNLLKTASGNGVAIGPILLGCA
                     RPVHVLTPSATVRRIVNMTALCVTDALSER"
     gene            5982..6947
                     /gene="thiL"
                     /locus_tag="VFA14_02140"
     CDS             5982..6947
                     /gene="thiL"
                     /locus_tag="VFA14_02140"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HZS80216.1"
                     /translation="MLSEFELISRYFTHPPAPNSRAALGAGDDCALLAPAPGMQLAVS
                     TDMLVEGCHFFPYADPYALGQQCLAVNLSDLAAMGAQPIAFTLALALPEAREDWLAPF
                     SRGLLRLADEHHCELIGGDTTKGPLAISITVFGEVPFTQALRRDAARPGDDIWISGAL
                     GDARLALAAYRRELTLAAEAHHTAARRMHQPMPRIALGIALRGIAHAAIDISDGLAGD
                     LGHILQRSRTGACLDVDTLPAGAILQRQSLDLRRRYTLSGGDDYELCFTAPENRREQV
                     LAAAKKTGTDVTRVGVIESMPGLRLVDAGGAPLDLALTSFDHFTA"
     gene            6944..>7232
                     /locus_tag="VFA14_02145"
     CDS             6944..>7232
                     /locus_tag="VFA14_02145"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HZS80217.1"
                     /translation="MTDTQIDTSARPTARFMLAHPAHFLAQGCGSGLSPIMPGTSGTL
                     FSWLTYAVLTWRWPGVFTPLTWLTVIGVGFVIGIWACRRTGIDLGAPDHGSM"
BASE COUNT         1593 a         2028 c         2014 g         1597 t
ORIGIN      
        1 cggcatgctc aaggagcagt ccgagacagc aatcaagaga caggcagagg gcctggagga
       61 gatgactgct gatcgcctat cggggagcaa tcgtccccaa tggcatgaaa tgaagaacca
      121 ctcagactat ggcccatatc caagcacggc tggacaacgg catcgcctcc tctcctaggg
      181 ctttcgatct cgtccaggcg agtcccggag gttcgctcac ggttcaaact gctcagctag
      241 gcgcccggac gcggcacaga ccgtctagtg agaccggaag catgcacttg atacctaaga
      301 ctcatactct gcggcaaaac cactaatcca gatggataca cagtgtgtat ccattatgct
      361 attgtgctgg aatgaagcca aaagactctg gaatgcggat cagggtcgag aaggaactac
      421 gcgatgcgtt cgtgcggtcc tgtcgagacc aaaatcaaac cgccgcaaat gtgctgcgcg
      481 atttcatgcg cacctacact gagcagcagg tgcagggaca aggcaattta tttgctattg
      541 cgaaacaaaa ataggacgca tagcagccct tatgcatacc tcttttgata agtccagtta
      601 attaagaaat gaactcagaa catctcgttt tatcgttatt ctcaggggca ggcggatttt
      661 cctatgggtt ttctcaggcc ggtctcaagc cgctattcgg tgccgagata aacgaggatg
      721 cttgtcagag ctatgagcta aatattcaaa gcccttgcca tgcagtggat ctgagtacag
      781 ttaatcccag tttattcaaa gcgatgaccg ggaagcgaac gccttttgca atcattgggg
      841 gaccaccatg ccaaggcttc agcactgcgg gaccacgcaa tgctaacgac ccgcgcaatc
      901 ggctcatatt caattatctc gctatcgtcg aggagctaaa gccgcgatgg tttatttttg
      961 aaaatgtcga aggcttgctc acgtctggag gtgggcaaga tgtcgcacga ctcgtgaaag
     1021 agtttattgg gctcggatat tcagttcggc ttcaaaaggt gaatcttgca gcctttggcg
     1081 ttcctcagac gagaaaaagg gtcatcatta ttggaaatcg cctagggata gactttgaat
     1141 ttccagagga aatttattcc tacgatagcg gcaaagtgaa aaaattatcg aacaaaccga
     1201 tggctcccac tcttgatgag gctatagcag gtttagggag tcctgtgctt gatcgatcaa
     1261 tatctcctta tttaccgaat gagcctgtca acgcatttga tgccgccatg cgacttgaaa
     1321 atggggggga aggggtaact caacatatcc aatgttccga ttcagtagag aaaattcatc
     1381 ttgaattgct aaaaccggga cagacgatga aggacttgcc ttctcaatac tggcacgaaa
     1441 gttttgagcg cagggccaat cgcagagtct cggatgggac gcccacagag aagagaggtg
     1501 gcgccccgtc tggcatcaag cgcctccatg gtaacttgca gtcattgaca ataacaggag
     1561 ctgcatctcg tgagttcatt caccctcgag aaaatcgccc tctgaccata cgtgagtgtg
     1621 ctcgtattca aacattccct gaccactata agtggaaagg gaacgctgcg agcgtaatac
     1681 agcaaattgg aaatgctgtc cctccacttg cggctagtgt attggcccgt catattcaac
     1741 atgtcgatgg tcagttcggc tcagatattc aggcatccca taaagatgga aaacctcgat
     1801 tgcttggctt tgtgctcacg gaggcgagcg gaatgagccc tgctttgaaa gccacgcaaa
     1861 gtctacttcg aaacgttcac caagaaaatc tagaactcaa tgaaagctaa aacaaaacgc
     1921 cttagcgcgg aaaaatctca gcaacttacc cgactcataa ccattacaaa aacggccacg
     1981 atgaaggccg taatggatcc gagtgagttg gcaaaagtca taacgctagt tgcaacagat
     2041 attggaaagg gtgatgagat ggcggaagcc tttccaaata tttgggaagg tatagctcct
     2101 ccaagcgact actactcact cactcttgat tggtttacca atcaaattgg tggagtttcg
     2161 gcatttgacg tcgttgatat gctggatcat ggggaaacac ttgacgaaga tttcctagct
     2221 tatttgaagt gtctgatgga gcttcacaaa cggcgaagaa aatacggact tatcctgcaa
     2281 cgccagccac ttcccaccat ggtgcaggtc tctccacgtg cgctaatgga atacggcccg
     2341 gactttcagc ctgaggcgct ggcttcttgg cttacttgga gaaaattttt ctacgaccta
     2401 gacaaccgtt cggcgcaaga gaccggatat ctcttcgagc ccattttagc cgctgcgatt
     2461 ggtggtgagc ctaagtctgc ccgagagtgt gtcgttcgcc gcagtgatga ccaggcaaaa
     2521 ggacgccaag ttgattgctg gaaggtcctg cctgacggca ctgcgctcgc ctatgagttg
     2581 aaactacgag tcactatcgc cgccagtggg caggggcggt ttggtgaaga gctgtcgttc
     2641 gctcaggatt gtcgcggctc aggggtcaag ccagtgctgg ttgtccttga tccgacagag
     2701 aacggcaagc ttactgggtt gcaggttgca tattgtgctg ctggtggtga tgcctatgtc
     2761 ggcgatgctg cttgggctca ccttgaagat gaggccggac cgaccatggc ggcgtttatt
     2821 gagcgatatg tccgcacacc agtcaatgct gtaacggcat ttgaaactag ccttgaaggc
     2881 gagccatcaa ggcgaagcat agctttgctc gacctggccg ccaagctcga aggtaacgac
     2941 ctgaccatta cattgggaca gcatcagcgc aagatcgagc gtcacgaaga cccgctgttg
     3001 tcagatgagg atgactcacc tgaggaatag cggacgaagg ggtaacgatc aattcggccc
     3061 caacatcgga agcaaaaagg gcagcccgcg tgggctgccc ttctcacttc aagtggtgcc
     3121 ggaaatagga atcgaaccat cattacgaat gacgcgctct accaactgag ctattccggc
     3181 aaaacctcaa aacttgttca aactgtgttc atactatctc gacctcatgc cgctggcgac
     3241 ctgaaagcct tgctgtgctt agcttcgagg ccgcgcatca taacatccgc gtcgcgatta
     3301 cgaatgcgct gctctaccga ctgagctaca gcgactactt ctcaaaactt gttcaaaccg
     3361 cgttcgttcg ttactgcggc tatgccgctg ggactcccaa agccttatat tgcttgcctt
     3421 gtaggttaga ttatctagag caacgttccg attccgaaaa aaatgtaccg ctttatcgcg
     3481 gcctccaatg cgtagcggtc aacgctccga caaggcatcc gtgacgcaca aggcagtcat
     3541 attgacgata cggcgcactg ttgccgatgg cgtcagaaca tgcaccggcc tggcgcagcc
     3601 cagcaggata gggccgatgg ccacaccatt gccggatgcc gttttcaaca ggttgtagac
     3661 gatgttggca gcgtcgatgt tcggcatcac cagcaggttg gcgtcgcctt tgagcggcga
     3721 atccggcatg agctgcgcca gcagtttcgc atcgagcgcg gtgtcaccgt gcatttcgcc
     3781 atcgacttcc aggtctgggg cctgctgctg tatcaatgcc agcgcggcgc gcattttgca
     3841 ggccgattcg ctgttactgg aaccgaagtt ggagtgtgac agtagcgcag cgcgcggatg
     3901 cagaccgaaa cggcgcattt cttccgccgc caggatagtg atttcggcca gttccgcggc
     3961 ggtcgggttt tcgttgacgt gcgtgtcaac cagcaccaac tgccgattcg gcaggatcac
     4021 gcaattcatc gcggcataca cattcacgcc tgcgcgcttg cctagtacct gatcgatgta
     4081 atgcacatgc aactgggtgg taccgtaggt gccgcagatc atgccgtcgg catcgccctt
     4141 gtagatcgcc atggcgccga tcagggtgtg acggcggcgc atttccagcc tggcgtactg
     4201 ctcggtgacg cccttgcgct tggtcattgc caggaaagtc tgccaatagt cgcgataacg
     4261 gttatcaaag ttcgggttga tgacttcaaa gtcagcaccc ggacgcaagc gcagacaaaa
     4321 cttcctgata cgctgttcga gtacttccgg acgtccaatc aggatcggcc gggccagacc
     4381 ttcgtcgacc acgacctgca cggcgcgcag cacacgttct tcttcgccct cggcgtagac
     4441 gatacgtttt ttggagtccg gtgctttctt tgcagcctga aagaccggac gcatgagggt
     4501 gccgctgtga tagacgcact gctccagctt gtcggcatag gcgtccatgt ctctgatcgg
     4561 acgggctgcc acaccggaag cttcagcggc acgcgccacg gctgtggcga tcttggtcat
     4621 cagccgcgga tcgaagggct tgggaatgat gtattccgga ccgaaggaca gatggggcat
     4681 gccataggtc gccgtaacga catcggattg ctcgacctgc gccagttcgg caatggcgtg
     4741 tactgcggca atctccatct cacgcgtgat cgtggtggcg ccggaatcca gcgcgccgcg
     4801 gaagatatac ggaaagcaga gaacgttgtt gacctggttc ggatagtcgg aacggccggt
     4861 ggcgatcacg gcgtcgtcac gcactgcctt gacgtcttcc ggcaggattt ctggcgtcgg
     4921 gttagccaat gccagcacca gtggacgcgg cgccattttc ttgaccatat cctgcttcag
     4981 cacaccgccg gcggacacac cgaggaaaat gtcggcgtcg gggatcacat cagccagggt
     5041 gcgcgcatca gtttcgcgcg caaaacgttc cttgtccgga tccatcagtt cggtacggcc
     5101 tttgtagacc acaccggcca ggtcggtgac gtaaatattc tggatcggga agccgaggtc
     5161 gatgatcagg tccaggcagg ccagcgctgc cgcgcccgcg ccggacacca ccagtttgac
     5221 ttccctgata tccttgccga cgaccttcag accgttcagg atcgcggaac cgacgatgat
     5281 ggcggtgccg tgctgatcat catggaaaac cggaatcttc atgcggtcac gcagtttgcg
     5341 ctcgatgtag aagcactcgg gcgccttgat atcttccaga ttgatgccgc cgaaagtcgg
     5401 ttccagcgcg gcaatgatgt cgcacagtcg atccgggtcg gtttcgttga tttcgatgtc
     5461 gaagacatcg atgccggcga atttcttgaa cagcacgcct ttgccttcca tcaccggctt
     5521 ggatgccagc ggaccgatat tgcccagacc aagcacggcg gtgccgttac tgatgaccgc
     5581 gaccagattg ccgcgcgcgg tgtatctgaa cgcgttggca ggatcggcga tgatttcttc
     5641 gcaggccgcg gcaacgcccg gagagtaggc cagagacagg tcacgctgat tggtcaatag
     5701 cttggtgggc gtgacgctga tcttgcccgg cgctggaaat tcatggtatt ccagcgctgc
     5761 ctgacgcagt tgctgacgta tttcttcttt cttgctgttc gtcgaatcca tactctacgc
     5821 tctctttttt aggcctgtcg aaggcttgtg attctagcag aggccagtac acgctgatag
     5881 cacggagcaa ctcttccatg ccgcaatgca gcattgcgca agccaatact gatgcggctc
     5941 tgtcagcaga atcggatcgg atattcgcta caatcacgtc catgctctcc gaatttgaac
     6001 tcatttcccg ctatttcacc catccgcctg cgccaaacag ccgcgccgcg ctcggcgccg
     6061 gcgacgattg tgcgctgctc gcgcctgccc caggcatgca attggccgtg tcaaccgaca
     6121 tgctggtcga aggctgccat ttttttccgt atgccgatcc ctatgcgctg ggacagcaat
     6181 gcctggcagt caatctgtcg gatctcgctg ccatgggcgc gcagccgatc gccttcaccc
     6241 tggcgctggc cttgcccgag gcgcgtgaag actggctcgc cccgttctca cgcggcctcc
     6301 tgaggctggc cgatgaacac cattgcgaac tgattggcgg cgacaccacc aaagggccgc
     6361 tggccatcag catcacggtg ttcggtgaag tgccgttcac ccaggccttg cgccgcgacg
     6421 ccgcccggcc cggcgacgac atctggattt ccggcgcgct cggtgatgcg cgcctggcgc
     6481 tggccgccta tcgccgcgaa ctgacgctgg ccgccgaagc gcaccacaca gccgcccggc
     6541 gtatgcatca gccgatgccg cgcatcgcgc tcggtatcgc cttgcgcggc attgcgcatg
     6601 ccgccatcga tatctcggat ggtctggccg gcgatctcgg ccatatactg caacgctcgc
     6661 gcaccggcgc ctgtctcgat gtcgacacct tgccagccgg tgcgatcctg caacgacaat
     6721 cgctcgacct gcgccgccgc tacacactct cgggcggcga tgattatgaa ctctgcttta
     6781 ccgcgccgga aaaccgccgc gagcaggtgc ttgctgcagc gaaaaaaacc gggaccgacg
     6841 tcacccgcgt tggtgtcatc gaatccatgc ctggcctgcg cctggtcgat gccggcggcg
     6901 caccgctcga cctcgccctc acttctttcg accatttcac tgcatgaccg acacacaaat
     6961 cgatacatcg gcgcgaccga ctgcgcgttt catgctggcg catccggcgc atttcctggc
     7021 gcaaggttgc ggcagcgggc tgtcgccgat catgccgggc acttccggca cgctattctc
     7081 ctggctgacg tatgcggtgc tgacctggcg ctggcctggg gtgttcacgc cgctgacctg
     7141 gctgaccgtg atcggcgtcg gtttcgtcat cggcatctgg gcctgccggc gcaccgggat
     7201 cgatctcggc gcgccggatc acggcagcat gg
//
LOCUS       DASRDH010000087         2100 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_540253,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000087 DASRDH010000000
VERSION     DASRDH010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_540253"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..549
                     /locus_tag="VFA14_02150"
     CDS             <1..549
                     /locus_tag="VFA14_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008332145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HZS80218.1"
                     /translation="DVMPRLRVNAMYAHALKQAKGEGLLNSQLQEAKWLIKNIRQRFD
                     TILRVSQAIVERQRSFFSHGVIAMRPLVLREIADTLGLHESTISRVTTRKYMLTPHGM
                     FELKYFFGSHVATEAGGEASSTAIRALIKQLIGAEDPRIPLSDSRIADVLAEQGMVIA
                     RRTVAKYREVLKIPPASFRKSL"
     gene            659..1012
                     /gene="raiA"
                     /locus_tag="VFA14_02155"
     CDS             659..1012
                     /gene="raiA"
                     /locus_tag="VFA14_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007877449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HZS80219.1"
                     /translation="MNLTISGHHLTLTPAIREYVQSKLTRVRRHFDHVIDINVILSID
                     SLTKKEKRQKAEINLHIKGKDLHAESIAHDLYAAIDMLIDKLDRQVIKHKNRMQAHHS
                     MAKRLPEAIMPAALS"
     gene            1184..1495
                     /gene="grxD"
                     /locus_tag="VFA14_02160"
     CDS             1184..1495
                     /gene="grxD"
                     /locus_tag="VFA14_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010402157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HZS80220.1"
                     /translation="MSEVQSWIKETVTRNPVVLFMKGTAQFPQCGFSGKAIQLLKASG
                     AKNIVTINVLENTDVRQGIKEYSNWPTIPQLYVDGEFIGGSDIMSEMFESGELQGLFK
                     A"
     gene            1522..>2100
                     /locus_tag="VFA14_02165"
     CDS             1522..>2100
                     /locus_tag="VFA14_02165"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0106141 - flavin prenyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HZS80221.1"
                     /translation="MPDPRRLIVAITGATGVIYGVRLLRVLRDSNIETHLLISEAGAL
                     TLHQELDMRRKEVEALADVVHSVRDVGASIASGSFTSCGMIAAPCSMKTLAAVANGLS
                     DNLISRAADVVLKERRRLVLMVRETPLNLAHLRNMVSVTEMGGIIFPPLPCFYHRPQS
                     IDDMIDHTLGRVLDLFAIPHTLTPRWNGDVVSK"
BASE COUNT          529 a          576 c          533 g          462 t
ORIGIN      
        1 gacgtcatgc cgcgcctgcg cgtcaatgcc atgtatgctc atgcattaaa gcaggccaaa
       61 ggtgagggat tgctcaattc gcaattgcag gaagccaaat ggctgatcaa aaacatacgc
      121 cagcgtttcg acacgatctt gcgtgtctcc caggcaattg ttgaacggca acgcagtttt
      181 ttctcgcatg gcgttatcgc catgcgtccc cttgtattac gcgaaatagc tgatacactg
      241 gggttacacg agagtacaat ttcccgtgtg acaacccgaa aatacatgct gaccccgcat
      301 ggcatgtttg agttgaaata tttcttcggc agccacgtcg cgacagaagc cggaggtgag
      361 gcatcctcta ccgcgatacg ggctctcatc aaacaactga taggagcaga agaccccagg
      421 attccacttt ctgatagcag gattgcagat gtgctggcag aacaggggat ggtgatagca
      481 cgccggactg tcgccaaata ccgcgaagta ctcaaaatcc ccccagccag tttccgcaaa
      541 tccctgtaag tttttttgta gtaaagtacc cgctgcaggg ttccagcgct atgctccaca
      601 ggcaaccgga cgatgcgtaa aaacctccag caatctcatt ataggtatag gaggctgtat
      661 gaatttgacc attagtggcc atcacctcac tctcaccccc gcaatccgcg aatacgtgca
      721 aagcaaactg acacgcgtca gacgccattt cgatcatgtc atcgacatca acgtgatcct
      781 cagtatcgat agcctgacaa aaaaagaaaa acgccaaaaa gcagaaatca accttcatat
      841 caagggtaag gatctgcatg ccgaaagtat cgcgcatgac ctgtatgctg cgatcgacat
      901 gctgatcgat aaactggacc gacaggtcat caagcacaag aaccgcatgc aggcccatca
      961 ttcgatggca aaacgcctgc cggaagcaat catgcccgca gcgctctcgt aacaggcaac
     1021 gagccggtga attggaccgg cgacagaaaa agggcgcggc agcgcccttt tttgtgtcag
     1081 cacatggtct ttcgacaggt tcatccagga atgcttgcat accgacgcag tgacacgctt
     1141 ttccctataa tgacggtatc gtgaattttt tgaaggtgta gtcatgagcg aagtccagtc
     1201 ctggatcaaa gaaacagtca cccggaaccc tgtcgtgctg ttcatgaagg ggacggcgca
     1261 atttccacaa tgcggctttt ccggcaaggc gattcaactg ctcaaagcca gcggcgcgaa
     1321 aaatatcgtc accatcaacg tgctggaaaa cactgatgtg cgtcaaggca tcaaggaata
     1381 ctcaaattgg ccgaccattc cgcaactgta tgtcgatggc gaattcatcg gcggttccga
     1441 tatcatgagc gaaatgttcg aatccggtga actgcaaggc ttgttcaagg cttgagtctt
     1501 tattgacggc gacacgtcca tatgccagat cctcgacgcc tgatcgtcgc catcaccggc
     1561 gcaaccggcg ttatctatgg tgtacgcttg ttacgtgtgc tgcgagacag caatatcgaa
     1621 acgcatctgc tgatctccga agctggcgcg cttactttgc accaggaact cgatatgcgt
     1681 cgcaaggagg tcgaagcgct ggccgatgtg gtccattcgg tgcgcgatgt tggcgcctcg
     1741 attgccagcg gctcttttac ctcctgcggc atgattgcgg ctccctgctc aatgaaaacg
     1801 ctagccgccg tagccaacgg actatccgac aatctcatct cacgtgccgc cgatgtcgtc
     1861 ctgaaagagc gccggcgact ggttctgatg gtgcgagaaa cgcccttgaa tttggcccac
     1921 ctgcgcaaca tggtcagcgt gacggaaatg ggcggcatca tttttccgcc tttgccctgc
     1981 ttctaccatc gtccgcaatc gatcgacgac atgatcgacc acacgttggg ccgggtactg
     2041 gatctgtttg ccatcccgca cacgctgaca ccgcgctgga acggagatgt ggtcagcaag
//
LOCUS       DASRDH010000088         2775 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_486608,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000088 DASRDH010000000
VERSION     DASRDH010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_486608"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..787)
                     /locus_tag="VFA14_02170"
     CDS             complement(<1..787)
                     /locus_tag="VFA14_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006465611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HZS80222.1"
                     /translation="MGKVTGFMEYGRLQEASEAPQSRIKHYKEFILHLGDAEAKMQGA
                     RCMDCGIPFCSNGCPVNNIIPDWNDLVYRGNYKQALDTLHSTNNFPEFTGRICPAPCE
                     AACTLNINDDAVGIKSIEHFIIDKGWENGWIAPQPAVVKTGRKVAVVGSGPAGLAAAQ
                     QLARAGHDVTVFEKNDRVGGLLRYGIPDFKMEKSHIDLRIEQMQAEGVIFRTGVLVGK
                     DFPANVTNWSRETVSPEQLKKDFDAVVIAGGAEQPRDLPVPGRE"
     gene            complement(901..>2775)
                     /locus_tag="VFA14_02175"
     CDS             complement(901..>2775)
                     /locus_tag="VFA14_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HZS80223.1"
                     /translation="KSNTGEGGEDPARDVPDANGDWRRSAIRQVASARFGVTSQYLVN
                     CTDLQIKMAQGAKPGEGGQLPGHKVTDYIATLRVSVPGVGLISPPPHHDIYSIEDLAQ
                     LIHDLKNVNPRASISVKLVSKTGVGTVAAGVAKAKSDHIVIAGHDGGTGASPLSSIKH
                     AGTPWELGLAETQQTLILNGLRNRIRVQADGQMKTGRDVVIGALLGADEFGFATAPLV
                     VEGCIMMRKCHLNTCPVGVATQDPVLRARFSGKPEHVVNFFFFVAEEVRQIMAQLGVR
                     TFNELIGRSDLLDRSRAIAHWKAQGLDFSRIFHQPASPTPYFHTEEQDHGLDKALDRK
                     LIAQAKMALEKGEKVSFISPIKNLNRTVGAMLSGEVARRYGNAGLPDDTIHIQLRGTA
                     GQSAGAFLTHGVTLDLIGEGNDYVGKGLSGGRIIVRPNTEFRGRAVDNIISGNTVLYG
                     ATSGEAFINGVVGERFAVRNSGAIAVVEGSGDHGCEYMTGGTVVVLGATGRNFAAGMS
                     GGIAYVYDEAGDFAGKCNTAMVALDKVLSNIEQEASTDRAIWHSLQRGGIGQTDEVIL
                     RGLIERHVKYTGSTRARYLLDNWAVSRSRFVKVFPTEYKRALSELHAVETSAKDRVPA
                     "
BASE COUNT          532 a          857 c          789 g          597 t
ORIGIN      
        1 actcacggcc cggcaccggc aggtcgcgcg gctgctcggc gccgccggca atcacgacag
       61 catcgaaatc ttttttcagc tgctccggtg atacggtttc cctggaccag ttggtgacat
      121 tggcagggaa atccttgccc accaatacgc cggtacgaaa aatcacacct tcggcctgca
      181 tctgttcgat gcgcagatcg atgtgcgact tttccatctt gaaatcgggg atgccgtaac
      241 gcagcaggcc accgacgcga tcattttttt cgaacacggt cacgtcatgg ccggcgcgcg
      301 ccagttgttg cgctgcggcc agaccggcag ggccagagcc aaccacggcg acttttctgc
      361 cagtcttgac gacggccggt tgcggcgcga tccagccatt ttcccaaccc ttgtcgatga
      421 tgaaatgttc gatagacttg ataccgacag catcgtcgtt gatgttgagc gtgcaggctg
      481 cctcgcaagg cgccgggcag atgcggccag tgaattcggg gaaattgttg gtcgaatgca
      541 gcgtgtccag cgcctgttta tagttaccgc gatagaccag atcgttccag tcggggatga
      601 tgttgttgac cggacagccg ttgctgcaga acgggatgcc acaatccatg cagcgcgcgc
      661 cttgcatttt cgcttctgca tcgcccagat gcagaatgaa ctccttgtaa tgcttgatgc
      721 gcgactgtgg cgcttcgctc gcctcttgca aacgcccgta ttccatgaag ccggttactt
      781 tacccatttt ccactcacat tcttcactaa ggacaggcgg gcggcgattc tgtccttatc
      841 agaatcgccg gccttgcctg tcgtttcgta ttgaccgttc acactgcctg gtgctgtctg
      901 ctatgcaggc accctgtctt tggcactcgt ttcgactgca tggagttcac tcaacgcgcg
      961 tttgtactcg gtcggaaaga ctttgacgaa cctgctgcgc gataccgccc agttatccag
     1021 caagtagcgc gcgcgcgtgc tgcctgtgta tttgacgtga cgctcgatca agccgcgcaa
     1081 tatcacttca tcggtctggc cgatgccgcc gcgttgcagg ctgtgccaga tggcgcgatc
     1141 cgtcgatgct tcctgttcga tgttggacaa caccttgtcc agtgcgacca tggccgtatt
     1201 gcatttgcct gcgaaatcgc cagcttcgtc atacacgtag gcaatgccgc cggacatgcc
     1261 tgccgcaaag ttacgtccgg tcgcgcccag cacgacgaca gtgccgccgg tcatgtattc
     1321 acaaccgtga tcgccgctgc cttcgacgac cgcaatcgcg ccagagttac gcaccgcgaa
     1381 acgctcgccg accacaccat tgatgaaggc ttcgccagaa gttgcgccgt acagcacggt
     1441 attgccggag attatgttgt cgacagcgcg accgcggaat tccgtgttcg gacgcacgat
     1501 gatgcgacca cccgacaagc ccttgccaac gtagtcgttg ccttcaccaa tcagatccag
     1561 cgtgacgccg tgcgtcagaa atgcgcctgc cgactgtccc gccgtacccc gcaactggat
     1621 gtgaatggtg tcgtccggca aacctgcgtt gccgtaacgc ctggccactt cacccgacag
     1681 catggcaccg acggtacggt tcaggttttt gatcggcgag atgaaagaaa ccttctcgcc
     1741 cttttccagc gccatcttcg cctgcgcgat cagtttgcgg tcgagcgcct tgtccagacc
     1801 gtgatcctgt tcttcggtgt ggaaataggg cgtcgggctg gccggttgat gaaaaatcct
     1861 gctgaaatcc agaccttgcg ccttccagtg cgcaatcgcc ctggaacggt cgagcaggtc
     1921 ggaacgaccg atcagttcgt tgaaagtgcg aacacccagc tgcgccatga tctgacgcac
     1981 ttcttcggcg acgaagaaga agaagttgac cacgtgttct ggcttgccgg aaaacctagc
     2041 gcgcaaaact ggatcctgcg ttgccacgcc gaccgggcaa gtgttcagat gacatttgcg
     2101 catcatgatg cagccctcga ccaccagcgg cgcggtggcg aaaccaaatt cgtccgcgcc
     2161 cagcaacgca ccgatgacga cgtcgcggcc agttttcatc tgaccgtctg cctgcacgcg
     2221 aatgcggttg cgcaaaccgt tgagaatcag cgtctgctgg gtttcagcca gaccgagttc
     2281 ccatggcgta ccggcatgct tgatcgagga cagcggcgag gcgccagtgc cgccgtcatg
     2341 accggcgatc acgatgtgat cggacttggc tttggcgaca ccggcagcaa ccgtgccgac
     2401 gccggttttc gacaccagct tgaccgagat cgaggcacgc ggattgacgt tcttcagatc
     2461 atggatcagt tgcgccagat cttcgatcga atagatatca tggtgcggcg gcggcgaaat
     2521 caggccgacg cccggcaccg acacgcgcaa tgtcgcgatg taatcggtca ccttgtggcc
     2581 cggcaactgg ccgccttcgc ccggcttggc gccctgcgcc atcttgattt gcaggtcggt
     2641 gcaattgacc aggtactggc tcgtcacgcc gaacctggcc gaggcgacct gacggatcgc
     2701 gctgcggcgc cagtcaccgt tcgcgtcggg cacgtcacgc gcgggatcct cgccgccctc
     2761 gccggtgttg gactt
//
LOCUS       DASRDH010000089         2863 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1527486,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000089 DASRDH010000000
VERSION     DASRDH010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2863
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1527486"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1907)
                     /locus_tag="VFA14_02180"
     CDS             complement(<1..1907)
                     /locus_tag="VFA14_02180"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008327388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HZS80224.1"
                     /translation="MRSSSPTTAPALQELTLTIDGMTCASCVGRVEKALHAVPSVREA
                     SVNLATERATVLAEPGVQTERLIAAVARAGYHAALPATVDTPGQAPALPAWWPVAAAA
                     LFTTPLILPMLLMPFGIAWQLPGTWQLLLATPVQFWLGARFYQAGWRALKARTGNMDL
                     LVALGTSAAYGLSLFTLLRQWQSQHAAHFYFETSAVVITLVLLGKWLEAHAKRQTTDA
                     IRALDALRPSKATVRRNGIDSDVSLAAIRTGDLIVVRPGERVAVDGIVREGWSHLDTS
                     MLTGESLPMEKQPGDKVTGGAINGAGLLLVETTAVGAETVLARIIRLVEHAQAAKAPI
                     QRLVDQVSAVFVPVVLVLALITLLGWGLIAGGWEQALLNAAAVLMIACPCALGLATPT
                     AIMAGTGVAARHGILIKDAEALETAHRIAVVAFDKTGTLTQGKPVLAAVQVIDAHPTS
                     EDRLLAIAAALQRNSEHPLAHAVMAAAQQKHIAPLVSDAGKAIPGRGTQALIDHHPTY
                     LGNTRWMQELGIPLDGQAALDTTAKEWERQGLTISWLAQDAGQGIALLGLLAFGDTIK
                     RSATRAVTRLHALGIRTIMLSGDNHGATAHVAQELGLSDFRAGILPADKAQAIGDLRK
                     NGIVVAMVGDG"
     gene            2022..2219
                     /locus_tag="VFA14_02185"
     CDS             2022..2219
                     /locus_tag="VFA14_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007878202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HZS80225.1"
                     /translation="MLTFDVQDMSCEHCVGAITRAIREVDANARVDVQIAGKRVMIDS
                     GVAAARFAQAIREAGYTPVAV"
     gene            2422..>2863
                     /locus_tag="VFA14_02190"
     CDS             2422..>2863
                     /locus_tag="VFA14_02190"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014618896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HZS80226.1"
                     /translation="MNKEQEALQIKITDPECRSFLARTLIWLVGTLALPLAGCGHMIA
                     QDAAYTIDTSIQSPNQNSRVRTLVLHYTAETLANSIALLTDPQKQTSSHYLVPDAANG
                     SGRFRIYALVPESRRAWHAGVSYWQGERMLNASTVGIEVVNLGFP"
BASE COUNT          548 a          831 c          923 g          561 t
ORIGIN      
        1 ataccatctc ccaccatggc gacgactatg ccgttcttgc gcagatcacc gatggcttgt
       61 gccttgtcgg ccggcagaat gccggcgcgg aaatcgctga ggccgagttc ttgcgccaca
      121 tgtgcagtgg cgccgtggtt gtcgccgctc agcatgatgg tgcgtatgcc cagagcatgc
      181 aggcgcgtga cggctcgcgt tgcactgcgc ttgatggtgt cgccgaaggc cagcaggccg
      241 agcagggcga tgccttgtcc ggcatcctgc gccagccagg aaatcgtcag accttgccgt
      301 tcccattctt tcgcggtggt gtcgagcgcg gcctgaccat cgagcggaat gcccagttcc
      361 tgcatccagc gtgtgttgcc gagataggtt ggatgatggt cgatcagtgc ttgcgtgccg
      421 cgtccgggga tggctttacc tgcatctgaa accagcggtg cgatgtgttt ttgctgtgcc
      481 gcagccatga cggcatgcgc cagcggatgc tcactgttgc gttgcagcgc agcggcaatg
      541 gcaagcagac gatcctcgct cgtgggatgg gcatcgatta cctgcactgc tgccagcacc
      601 ggcttgcctt gcgtcagcgt gcctgttttg tcgaaggcga cgacggcgat gcggtgcgcg
      661 gtttccagcg cctcggcatc cttgatcaat ataccgtgac gcgcagccac gccggtgccg
      721 gccatgatcg cggtcggcgt ggcgagtcca agcgcgcatg gacaggcgat catgagtacg
      781 gcagcagcgt tgagcagcgc ctgttcccat ccgccagcaa tcagtcccca gccgagtagc
      841 gtgatcagcg ccagcaccag caccaccggc acgaacactg cgctgacctg gtcgaccagg
      901 cgctggatcg gtgccttggc ggcctgcgca tgttcgacca ggcgaatgat acgcgccagc
      961 acggtttcgg cgccgacggc ggtggtctcg accagtaaca ggccagcgcc gttgatggcg
     1021 ccgccggtga ctttgtcgcc aggctgcttt tccatcggca ggctttcacc ggtgagcatg
     1081 gaggtgtcga ggtggctcca tccttcgcgc acgatgccat cgacggcgac gcgctcaccg
     1141 gggcgcacca cgatcagatc gccggtacgg atcgccgcga gggacacgtc gctgtcgata
     1201 ccgttgcgtc gcacggtagc tttgctcggt cgtagcgcat cgagcgcgcg gatcgcatcg
     1261 gtagtctggc gtttggcgtg cgcctccagc catttgccga gcagcaccag cgtgatgacg
     1321 actgccgatg tctcgaagta gaagtgcgcg gcatgctggc tttgccattg cctgagcaag
     1381 gtgaatagcg acaatccata ggcggcgctg gtgccgagcg cgaccagcag atccatgttg
     1441 ccggtccgcg cctttagcgc gcgccatcct gcctggtaaa agcgcgcgcc gagccagaac
     1501 tgcaccggcg ttgccagcag cagctgccat gttcccggca actgccaggc gatgccgaaa
     1561 ggcatcagca gcatcggcaa tatcagcggc gtggtgaaga gcgcggcggc agcgaccggc
     1621 caccatgcgg ggagggcagg cgcctgtccg ggcgtatcga cagtcgccgg cagcgcggcg
     1681 tggtaaccag cgcgcgcgac ggcggcgatc agtcgttcgg tctgcacgcc tggttcggcc
     1741 agcacagtgg cgcgttcggt cgccaggttg acgctggctt cgcgcacgga tggcacggcg
     1801 tgcaaggctt tttcgacacg gccgacgcag gaagcgcagg tcatgccgtc gatggtgagc
     1861 gtcagttcct gcaacgcagg cgcagtggtc ggggatgagg agcgcattgt cagtgtcagc
     1921 gcgtgggatg gagggagcat gatagagtcg gggcttattt tgacataaac aggccagctc
     1981 gaccctggcc cgtatcacgc attgaaaaaa ggaagaacac catgctgaca tttgacgttc
     2041 aggatatgag ctgcgagcat tgcgtcggtg ccatcacgcg cgccatcagg gaagtcgacg
     2101 ccaatgccag ggtcgatgtg cagatcgccg ggaagcgcgt catgatcgat agcggcgttg
     2161 ccgccgcaag attcgcgcag gcgatcagag aagcgggtta tacgcccgtc gctgtttgag
     2221 cagtgttcca tcgcagctac cgccgggtct cttggcgctc tctttttagg tgtggcaggt
     2281 gctggtctgg atgctgttgg ttattaaaga gctacaatct cttcagcaag tactttgaaa
     2341 accagcagtt tcagcaaaag atccttgact gacagaagca gcagggaaag tggtgggctg
     2401 ctacgtcaaa aggacaatgg catgaataaa gagcaagaag cactacaaat caaaatcact
     2461 gacccggaat gccgcagttt tctcgcccgg actttaatat ggttagtagg cacacttgcg
     2521 ttgccacttg caggatgtgg gcacatgatt gcgcaggatg cagcttatac gatcgatacg
     2581 tcgatccagt caccgaacca aaattcgcgc gtacgcacac tggtactgca ctataccgcc
     2641 gaaacgcttg ccaactcgat tgcgttgctg accgatccac aaaagcagac aagttcacac
     2701 tacctcgtgc cggatgcggc caacggcagc gggcgcttcc gtatctatgc actcgtgccg
     2761 gaatcgcggc gcgcatggca tgccggggtt agctactggc agggtgagcg gatgctcaat
     2821 gcaagcacgg tcggtattga ggtggtcaat ctcggttttc ccg
//
LOCUS       DASRDH010000090         2088 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_833643,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000090 DASRDH010000000
VERSION     DASRDH010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_833643"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..673)
                     /locus_tag="VFA14_02195"
     CDS             complement(<1..673)
                     /locus_tag="VFA14_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008328239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HZS80227.1"
                     /translation="MTQAFLPFSRPVIDEATIAAVGEVLRSGWITSGPKVLAFEKALS
                     DYLGGRIVRSFNSGTCTMEIALRIAGIDAGDEVITTPISWVATANVIIEVGATPVFVD
                     IDPVTRNIDLEQLEAAITARTRALIPVYLAGLPVDMERLYAIAGKHHLRVIEDAAQAL
                     GSTWNGKRIGAFGDFVSFSFQANKNITSSEGGCLVVNDAEEARLAEKYRLQGVTRSGF
                     DGLEVD"
     gene            complement(705..1076)
                     /locus_tag="VFA14_02200"
     CDS             complement(705..1076)
                     /locus_tag="VFA14_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HZS80228.1"
                     /translation="MNLATFGFIFAGICLNAVAQLLLKAGTNAVGAIHLSADNWFATG
                     VRLITQLPIIGGLCCYVISVGVWIIGLSRVDVTLAYPLLSLGYIINAIGAWYFLGEAI
                     SAQRLLAITVIIAGVILLARS"
     gene            complement(1073..>2088)
                     /locus_tag="VFA14_02205"
     CDS             complement(1073..>2088)
                     /locus_tag="VFA14_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZS80229.1"
                     /translation="FLLVSLKNPEFPQFFFIHEHVQRFTTKIHSRAGPWYYFIPILLL
                     GILPWLGALLPSLLGSLRDEQNRGGFQPRKMLLVWSVFIFVFFSMSGSKLPSYILPIF
                     PALALLIADYLERASCKAIVLSASIVALPCAIAMAFIPRVPSLAKDAYSLPLVTAHVP
                     WIYAAAIVAFIGAILAIRCARGQKDLAMVWLAAGAFLSGQLLLLGHDPQGRYSAGIDH
                     VPALQAELTPETPIYLVGRYEQVLPFYLGRTMTLVGHADEMAFGLQQEPWLWLPTLDA
                     FVARWVADHAADRKDLAIMAPAIYRELQQRGVPMRLIGQDPRRVIVANDPASADRNRK
                     TER"
BASE COUNT          458 a          584 c          675 g          371 t
ORIGIN      
        1 cgtcgacttc caggccgtcg aagccgctgc gggtaacgcc ttgcagacgg tatttttccg
       61 ccaggcgcgc ttcttcggca tcgttgacga ccaggcagcc gccttcggaa ctggtgatgt
      121 tcttgttggc ctgaaaactg aatgaaacga agtcgccgaa tgcgccgatg cgcttgccgt
      181 tccaggtcga gccgagcgcc tgcgcggcat cttcgatgac gcgcagatgg tgttttccag
      241 cgatggcgta caggcgctcc atatcgaccg gcaagcctgc cagatagact ggaatcagcg
      301 ctctggtgcg cgcggtgatg gcggcttcca gttgctccag atcgatgttg cgtgtgaccg
      361 gatcgatgtc cacgaacacc ggcgtggcgc cgacttcgat gatgacgttg gcggtggcca
      421 cccacgagat gggggtggtg atgacttcat cgccggcgtc gatgccggca atgcgcaggg
      481 cgatttccat ggtacaggtg ccggaattga agctgcgcac gatacggccg ccgagataat
      541 ccgacagtgc tttttcgaac gccagtacct tgggaccgct ggtgatccag cccgagcgca
      601 atacttcacc gacggcggca atggtggctt cgtcgatgac cggtctggaa aacggcagga
      661 aggcttgagt catgaagtac tcacgagaaa tcgagtggtg aaaatcagct tctggccagc
      721 aggatgacgc cggcaatgat gaccgtaatg gccagcagcc gttgcgccga gatggcttca
      781 ccgagaaaat accaggcgcc gatggcgttg atgatatagc ccagcgacag caaggggtag
      841 gccagggtca cgtccacacg tgacaggcca atgatccaga cgccgacaga aatgacataa
      901 cagcagaggc cgccgatgat cggcagttgc gtgatcagcc taacgccggt ggcgaaccag
      961 ttgtcggcgc tcaggtggat tgcgccgacg gcgttggtgc cggccttgag tagcaactgg
     1021 gcgactgcgt tgaggcagat gccggcgaaa atgaaaccaa aggtggcgag gttcatcgtt
     1081 cggttttcct attcctgtcg gcggacgcgg ggtcgttggc gacgatcacc cggcgcgggt
     1141 cctgtccaat caggcgcatc ggcacgccgc gttgttgcag ttcacgatag atggcggggg
     1201 ccatgatggc taggtctttt ctgtcggcgg catggtcggc gacccaacgg gcgacgaagg
     1261 catcgagcgt cggtaaccac agccagggct cctgctggag accgaatgcc atttcatcgg
     1321 catgcccgac cagcgtcatg gtgcggccca ggtagaacgg cagcacctgt tcgtagcggc
     1381 cgaccagata tatcggtgtt tccggcgtga gttcagcctg taacgccggt acatgatcga
     1441 taccggctga atagcggccc tgcggatcgt ggcccagcag cagcaactgt ccgctgagga
     1501 aagcgccggc ggccagccag accatggcca gatctttctg accgcgcgcg cagcgaatag
     1561 ccagaatcgc accgatgaag gcgacgatcg ccgcggcata aatccacggg acgtgcgccg
     1621 tgaccagcgg tagcgaatac gcatctttgg ccagcgacgg cacgcgtgga atgaaggcca
     1681 tggcgatggc gcaaggcagc gccacgatcg atgccgacag cacgatggct ttgcaggaag
     1741 cgcgctccag atagtccgcg atcagcagcg ccagcgccgg gaaaatcggc agaatatagg
     1801 acggcagctt ggaacccgac atgctgaaga agacaaaaat gaacaccgac cagaccagca
     1861 gcatcttgcg tggctggaat ccgccgcggt tctgctcatc gcgcaggcta cccaagagac
     1921 tcggcagaag cgcacccagc cacggcagga tgcccagcag tagaatcggg atgaagtaat
     1981 accaggggcc ggcgcggcta tgaatcttgg tggtgaagcg ctgaacatgc tcatggatga
     2041 aaaagaattg cggaaattcc ggatttttca gcgataccag caggaacc
//
LOCUS       DASRDH010000091         3060 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_700978,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000091 DASRDH010000000
VERSION     DASRDH010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 3060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3060
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_700978"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..174
                     /locus_tag="VFA14_02210"
     CDS             <1..174
                     /locus_tag="VFA14_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007882563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 8"
                     /protein_id="HZS80230.1"
                     /translation="LLAHTPDWRWLNGREDSPWYPSMRLFRQQSRGDWPSVITKVATE
                     LSFSRDKKTQHLS"
     gene            482..1570
                     /locus_tag="VFA14_02215"
     CDS             482..1570
                     /locus_tag="VFA14_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016833920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HZS80231.1"
                     /translation="MPSIINTNLPSLNTQRSLNASQGSLNTAIQRLSSGLRVNSSRDD
                     AAGLAIADRMNTQVKGMIVGQRNANDGISLAQTAEGALSSVTNNLQRMRELAVQAANG
                     SNSTRDRKTLNDEYSQLSAEVFRVLNSTQFNGLNLFTGIGTGASAGVGSDAANVSASL
                     TMTFQVGPNVVDNKLVDQISISLLDLSNSQTIANVVGTSAPSLIGIGDTTQVDIAQAN
                     LTAAKIALDELFTSSSTMTDQELASSAVTLQSSIDSAQLSLDEATAAPDAQSGAQKAI
                     KNLDQAISLISSQRAELGAMQNRFIAVINNLQISGENITSARSRIMDTDYAEETANLT
                     RGQILQQAGIAMLAQANAAPQTVLALLK"
     gene            1657..2028
                     /locus_tag="VFA14_02220"
     CDS             1657..2028
                     /locus_tag="VFA14_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007882566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlaG"
                     /protein_id="HZS80232.1"
                     /translation="MSISALPPDIAVAGNSGFSRPMQPIYYPPTVSTLPWPPPVVGTL
                     PLFPPSENDIASTVEELNKFISQSTPSITFSQDDDSGKTIIKVIDTTDNTVLRQIPSQ
                     EALHIAKSLDKLQGWLINKKA"
     gene            2180..>3060
                     /locus_tag="VFA14_02225"
     CDS             2180..>3060
                     /locus_tag="VFA14_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar cap protein FliD N-terminal
                     domain-containing protein"
                     /protein_id="HZS80233.1"
                     /translation="MATSTGTITTSAGPLDVATLVSKLMDSERTMLNPLTRQASSYNA
                     LISAYGSLKNALSTYQSTLNDLTAASFSSQKASISNTGTGTTLTTDPFTANVNSTDST
                     TALTQIIQSAGFPGSQTFNAGDTLAIKVGTNSPTFITLQDDTTLVGVRDAINAAKADV
                     TASITTDGSGDHLVLESNAAGTANTIKVQANNSLSSLAYDPGNPTSGTVTQIQAPRDA
                     TAATSGSYTVAISQLAQAQKITSAGFASDATFNSGLLAIKTSKGSTAIITPTTNTLAG
                     VRDAINASSAGVNATII"
BASE COUNT          832 a          905 c          707 g          616 t
ORIGIN      
        1 ttattggcgc acacccctga ctggcgctgg ctcaacgggc gcgaagactc tccctggtac
       61 ccaagcatgc gcctgttcag gcagcaaagc cgtggcgact ggcccagcgt catcacaaaa
      121 gtagcaacag aactctcttt ctcaagagat aaaaagacgc aacacctgag ttaatcaatc
      181 aaccatcctg gcaaacgatt gcattgcgcc aacgccaaca cagcatgttt ttggaaattt
      241 tatcaatatt cttcactaaa gttttcctga ataacgtcga aataagacta gcttgacaga
      301 aacgcgtagt tttgcgtcgg gcaacatggg aagaaaacct tgcaatagat attaaacttt
      361 ttccgatatc gtccgatata gcttattaac ggcggtttga atgcctgatg ttttggataa
      421 aaaccaaagt tataagcact gggacccgga ttgatcggcc tcgaatctta ggagaattag
      481 catgccatct attattaata ccaatcttcc gtcgctgaac acacaacgca gcctgaacgc
      541 ctcacagggg tcgctgaata ctgcgattca acgcttgtct tccggcctgc gtgtcaacag
      601 ctcgagggac gatgctgcgg gcctggcaat tgccgaccgc atgaatacac aggtcaaagg
      661 tatgatcgtt ggacagcgta acgccaacga tggtatttct cttgcgcaaa ccgctgaagg
      721 cgcactgagc agtgttacca acaacctgca acgcatgcgc gaactggcag ttcaggcagc
      781 aaacggctca aacagcaccc gggaccgcaa gaccctcaat gatgaataca gccaattgtc
      841 ggccgaagtc ttccgcgttc tgaacagcac ccaattcaac ggcctgaacc tgttcaccgg
      901 cattggtacc ggcgccagcg ccggcgtcgg ctccgatgcg gctaacgttt ctgcttcgct
      961 gacaatgact ttccaggttg gtcccaacgt tgtcgacaac aagctggtag accagatcag
     1021 catctcgctg ctcgacctat ccaacagcca aacaattgcc aacgtcgtcg gcactagcgc
     1081 tcctagtctg atcggcatag gtgacaccac acaggtcgac atcgcccaag ccaacttgac
     1141 agcagccaaa attgctctgg acgaattgtt tacaagcagt tcgacaatga ccgaccaaga
     1201 actggcttcg tcggcagtga cgttgcagtc cagcatcgac tcggcgcagc tgtccctgga
     1261 cgaagcaact gccgctccag acgcacaaag tggtgcgcaa aaggcgatca agaacctgga
     1321 ccaggcaatc agcctgatct cgtcccaacg tgcagaactc ggcgccatgc agaaccgttt
     1381 catcgccgtg atcaacaacc tgcaaatttc gggcgaaaac atcacctcgg cacgcagccg
     1441 tatcatggat accgactatg ctgaagaaac tgcgaacctc acacgtggac agattctgca
     1501 gcaagcaggt atcgcgatgc tggcacaagc gaacgctgcg ccacaaaccg tcctggcatt
     1561 gttgaaataa gcctggaatc taaccatgca ggatgaggcg cggggaatat gtatgcgtat
     1621 gtatttcccg cgcttttttg aaaaaggaat aagatcatgt cgatttccgc actcccacct
     1681 gatattgctg tagccggcaa ttctggtttt tccagaccga tgcagcctat atattaccct
     1741 ccaacggtat cgactctgcc atggccccct ccagtagtag gaacgctgcc attatttccc
     1801 ccctccgaga acgatatcgc atctacggtc gaagaattga acaaattcat aagccagagc
     1861 acccctagca tcaccttctc gcaggacgac gatagtggca agacgatcat caaggtcatt
     1921 gacacgacag acaacacggt tttgcgccag attcccagtc aagaagcgct tcacatcgct
     1981 aaatcgcttg acaaactgca aggctggctg atcaacaaaa aagcctgaat ctgatatgaa
     2041 ctgccggcac gagattgcat gtgcaaacaa aatatttgcg catgcaatct ctcgtttcgg
     2101 tgcctggaca attcagcacc cgtcacccag cccacggaaa taagtgcatc gtattgctat
     2161 ttagaaaaca ggaggacgca tggccacttc cactggaacg atcaccacat cagcaggccc
     2221 tcttgatgtt gccactctcg tctcgaagct gatggacagc gaacgcacca tgctgaaccc
     2281 gctgacaagg caggccagca gctacaatgc gctgatttcc gcctatggca gtctgaaaaa
     2341 tgctctctct acctatcaaa gcacgctgaa cgatttaacg gcagcgagct tcagttcaca
     2401 gaaagccagc atatccaata caggcacggg cacgacgctc accacagatc cgtttaccgc
     2461 gaacgtcaat agcactgact cgaccacagc actgacacaa atcatccagt ccgcaggttt
     2521 tccaggctcc caaacattta atgccggcga cacgctggca atcaaagtcg gcacaaattc
     2581 accgacattt atcaccctcc aggatgatac aacgctggta ggcgtgcgcg atgccatcaa
     2641 cgcagcaaaa gccgacgtga ccgccagcat cacaaccgac ggtagcggcg accatctggt
     2701 cctggaatcc aatgcggccg gcacggccaa caccatcaag gtacaagcca ataacagcct
     2761 gtcttccctg gcctacgacc cgggcaaccc gacgtctggc acagtcacgc aaatccaggc
     2821 accgcgtgat gcaacggcgg ctacttcggg aagctatacg gtcgccatat cgcaactggc
     2881 acaagcccag aaaatcacgt cagccggctt tgcctctgac gccacgttca attccggctt
     2941 gctggcgatc aagaccagca aaggctccac cgccatcatc acccccacaa ccaacactct
     3001 ggcaggcgtg cgcgatgcca tcaatgccag cagtgccggc gtcaatgcaa cgatcatcag
//
LOCUS       DASRDH010000092         2490 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_988773,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000092 DASRDH010000000
VERSION     DASRDH010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2490
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_988773"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1220)
                     /locus_tag="VFA14_02230"
     CDS             complement(<1..1220)
                     /locus_tag="VFA14_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013236210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HZS80234.1"
                     /translation="MAISCLMPACAQQRLPANATDDDIFLALRDAARDGDAATAISLA
                     ARLPDYVIASYVDYYRLKPRIRDLIAPVSEIRGFLTRYDGSAIADRLRNDWLLALGKA
                     GDWATFDEQYPLFVLDDDVQVKCFALSSRALKGQNVANEARTLLTGPRDYGQGCSDLI
                     STLAQNHQFGENDLWAQIRQAVETGSVTVARRAVVFTDSDDISLQQVIDKPARKLVRG
                     PGQGRAAHELYIIAIGRVAKDNPQKAAKFLSGAAGLTAQERAIAWAEIALPASIKLMS
                     EAVDYWRKAGNAPLSYKAYQWKARTALRAGDWKTLAATIETMPAALRNDITWIYWLAR
                     AQKEQGQTVSAQRLFRSIASSLSFYGQLATEELGRKIVIPPGPVLLTQAEIAPMTQNA
                     GFRRALKFYALNMR"
     gene            1292..1885
                     /locus_tag="VFA14_02235"
     CDS             1292..1885
                     /locus_tag="VFA14_02235"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007879414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HZS80235.1"
                     /translation="MAISRIARKTVPTDATDSEKSLLRLHLLAARKVMTPAAKAAAET
                     AICTRLLAWLRRHPVASLGVYYPIRQEPNLYPAYSALSTQGVQLSLPIIRGKDLPLEF
                     VRWTPGEMLVKDAMGTSAPPHGETVRPHALLIPCLGFNTAQMRLGYGGGFYDRTLTPA
                     PRPLAIGVAYADARVDFAAQAHDIALDLIITEAIAVP"
     gene            complement(1869..>2490)
                     /locus_tag="VFA14_02240"
     CDS             complement(1869..>2490)
                     /locus_tag="VFA14_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HZS80236.1"
                     /translation="VPKFRQQIRDGGPVTLTHPDMTRYFMSIPEAAQLVIQASAMAKG
                     GDVFVLDMGQPVRIIDLACRMIELSGLTVKDETNPDGDIAIEITSLRPGEKLFEELLI
                     GNDPKPTSHPRIMKAHEDFLPWDELERKLDALRTALQVNDVSVVRRLLQRLVTGYTPS
                     DDIVDWVHLEQESEDRTGTCSSARMSKAHEKTQPLSGPRDVFTVLQ"
BASE COUNT          523 a          730 c          707 g          530 t
ORIGIN      
        1 taacgcatgt tcagcgcata gaatttgagc gcacgcctga aaccggcgtt ctgtgtcatc
       61 ggcgcgatct cggcctgggt cagcagcacc ggtccgggag gaatcacgat tttccggcca
      121 agctcttcag tcgccagttg accatagaaa ctgagactgg aagcaatcga acggaacagg
      181 cgctgtgccg atacggtctg gccttgttct ttttgcgcac gcgccagcca gtagatccaa
      241 gttatatcat tacgtaacgc agcaggcatg gtttcgatgg tggccgcgag cgtcttccag
      301 tcgccggcgc gcaaggcggt tcgcgccttc cattgatacg ccttatagga caacggcgca
      361 ttcccggcct tgcgccagta atcgaccgct tcagacatca gctttatcga ggccggcaag
      421 gcgatctctg cccaggcgat ggcccgttcc tgcgccgtca gaccagcagc gccggagaga
      481 aacttcgcag ctttctgcgg attgtctttg gcaacgcgac caatggcaat gatatacagc
      541 tcatgcgcgg cgcgcccctg tcctgggcca cgcaccagct tccgcgccgg cttgtcgatg
      601 acttgctgca ggctgatatc atcactatct gtgaaaacca ccgcgcggcg cgcgacagtg
      661 acgctgccag tttcgaccgc ctggcggatc tgcgcccaca aatcgttttc accgaactga
      721 tgattttgcg ccagcgtact gatcaaatcg ctgcaaccct ggccgtaatc cctggggccg
      781 gtcagcaggg tgcgcgcctc attggcgacg ttctgcccct tgagggcgcg ggaactgagg
      841 gcgaaacatt taacctgtac gtcatcatcg aggacaaata aaggatactg ttcatcgaaa
      901 gttgcccaat cgccagcctt gcccagtgcc agcagccagt cattacgcag gcggtcggca
      961 atagcgctgc cgtcatagcg tgtgagaaaa ccgcgaatct cggaaaccgg tgcgatgaga
     1021 tcccgtatgc gcggtttgag ccgataatag tcgacatagg aagcgatgac ataatccggc
     1081 aggcgcgcgg ccagactgat cgcagtggcg gcgtcgccat cgcgcgcggc atcgcgcagg
     1141 gcaagaaaga tgtcatcgtc cgtagcattg gcgggaagac gctgctgcgc gcaggccggc
     1201 atgagacaac tgatcgccat ggcaacggcg caagtcagtg cggccaaaca tttctttaac
     1261 tgcatgttga tacgacctta gagtagaaca gatggcgatt tcaaggatag cacgcaagac
     1321 ggtgccgacc gatgcgaccg attcggaaaa atccttgctg cgcctgcatt tgctggccgc
     1381 ccgtaaagtc atgacgcctg ccgccaaggc cgctgcggaa accgccatct gcacccgcct
     1441 gctggcctgg ctgcggcgac acccggtcgc cagtctcggt gtctactacc cgatccggca
     1501 ggaaccgaat ctgtatccgg cctatagcgc gctcagcaca caaggcgtac agctgtcgtt
     1561 gccaatcatt cgcggaaaag acctgccgct ggaattcgtg cgctggacgc caggcgaaat
     1621 gctggtcaaa gacgccatgg gaacgtcggc gccaccgcat ggagagacag tacggccaca
     1681 cgctctgctg attccttgcc tgggattcaa taccgcgcag atgcggctgg gctacggcgg
     1741 cggtttctac gatcgcacgc taacgccagc gccgcgtccg ctcgctatcg gtgtcgctta
     1801 tgcggatgcg cgggtcgatt ttgcagcgca agcgcacgat attgcgctgg atttgatcat
     1861 cactgaagct attgcagtac cgtgaagacg tcacgcggcc cggacaaggg ctgtgttttt
     1921 tcatgcgcct ttgacatccg cgctgaggaa catgtcccag tacggtcttc ggactcctgc
     1981 tccagatgca cccagtcgac aatgtcgtcg ctgggtgtat aacctgtcac caatcgttgc
     2041 agcaagcgtc tgactacgct gacgtcattg acctgcaacg cggttctcaa cgcatccagc
     2101 ttgcgttcca gttcatccca gggaagaaag tcttcatggg ctttcatgat tctgggatgt
     2161 gatgtcggct tggggtcatt accgatcaac agttcttcaa acagtttttc cccagggcgc
     2221 aagctggtga tttcgatagc gatatctcca tccgggtttg tttcatcttt gacggtcagg
     2281 cccgaaagct cgatcattct gcaagcgagg tcgataattc tcaccggctg ccccatatcg
     2341 agaacaaaga catctccacc tttggccatt gcgctggcct gaatcaccag ctgcgcggct
     2401 tcaggtatgc tcatgaagta acgtgtcatg tcaggatgag tcagtgtcac tggaccgccg
     2461 tcacgaattt gctgccggaa ttttgggacg
//
LOCUS       DASRDH010000093         4130 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1539901,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000093 DASRDH010000000
VERSION     DASRDH010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 4130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4130
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1539901"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..801
                     /locus_tag="VFA14_02245"
     CDS             <1..801
                     /locus_tag="VFA14_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HZS80237.1"
                     /translation="AAIQGAALSGDRKDLLLLDVTPLSLGIETMGGVMTKMIKKNTTI
                     PTKFSQVFSTADDNQPAVTIKVYQGEREMAAGNKGLGEFNLEGIPPAPRGTPQIEVTF
                     DIDANGILHVGAKDKATGKENKITIKANSGLTEEEIEKMVRDAEANAEEDKRMKELAE
                     TRNQGDALVHSTRKSLTEYGDRLESGEKGAIEAAVKELEEVLKGNDKAAIDAKTAAVS
                     VAAQKLGEKMYADQQAQAAASGAGAGAASAGEAPKHDDVVDADFKEVK"
     gene            complement(837..974)
                     /locus_tag="VFA14_02250"
     CDS             complement(837..974)
                     /locus_tag="VFA14_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS80238.1"
                     /translation="MFYNHFIVASPRKLENQPQTNDMKKPRDDLATVWLGVLLYHGCR
                     A"
     gene            973..2091
                     /gene="dnaJ"
                     /locus_tag="VFA14_02255"
     CDS             973..2091
                     /gene="dnaJ"
                     /locus_tag="VFA14_02255"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006417099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HZS80239.1"
                     /translation="MAKRDFYDILGLAKNATEDDIKKAYRKLAMKYHPDRNPDSKSAE
                     EKFKEVKEAYEILSDPQKREVYDRYGHAGVDPNMGGGAGAGGFADAFGDIFGDIFGGG
                     GRGRRSESQVYRGADLRYNLEITLEQAAHGFDTTIRVPSWDECDTCHGSGAKPGTSPT
                     TCAACGGHGQVRMQQGFFSIQQTCPKCHGSGKIIPDPCSTCAGAGRIKRNKTLEVKIP
                     EGIDDGMRIRSSGNGEPGMNGGPPGDLYVEIHIKPHKVFRRDGDDLHCEMPISFTRAA
                     LGGDIEAPTLSGKATFTIPEGTQSGKIFRLRSKGIKGVRSGYAGDLFCHVVVEIPVKL
                     TERQKELLHEFEKLTVEGGAKHNPQAKTWMDKVKEFFE"
     gene            2250..2885
                     /gene="pncA"
                     /locus_tag="VFA14_02260"
     CDS             2250..2885
                     /gene="pncA"
                     /locus_tag="VFA14_02260"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012328688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HZS80240.1"
                     /translation="MKRTDDVLLVIDVQNDFMPGGALAVSDGDVIVPVINALAARFDQ
                     VVLTQDWHPREHLSFAANHAGAVPFSSVALPYGEQVLWPVHCVQQTSGASFHRDLDVR
                     SARGAVIRKGHNAQVDSYSAFVEADRTTHTGLADYLRELGAKRVWCCGLATDFCVVRS
                     ALDARAAGFDAAVIDDACRAIDLSGSMARAWEQMQAAGVVLVTSDGARPKA"
     gene            complement(3004..3831)
                     /gene="panB"
                     /locus_tag="VFA14_02265"
     CDS             complement(3004..3831)
                     /gene="panB"
                     /locus_tag="VFA14_02265"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HZS80241.1"
                     /translation="MAAYLQDAPNPRKQPVTIPVLQAMRDKGEKISVLTCYDASFAAL
                     MERCGVDMVLIGDSLGMTCQGHQSTLPVTIDDIAYHTACVARGNRTMMIAADMPFGTY
                     GSPPAAFDNAVKLMQAGAQMVKLEGGAWLADTVRLLTERGIPVWAHLGLMPQSVHQLG
                     GYKVQGKTRESAELLHADAAALQTAGASLLLLEAVPATLGAEITAQRHIPTIGIGAGV
                     DCSGQVLVMHDMLGVFAGHQAKFVKNFMEGAASVEEAVRAYVIAVRNQNFPAAEHCF"
     gene            3935..>4130
                     /locus_tag="VFA14_02270"
     CDS             3935..>4130
                     /locus_tag="VFA14_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HZS80242.1"
                     /translation="MRQYLDFMRHVYEHGTDKTDRNGTGTRSVFGYQMRFDLQDGFPL
                     LTTKKLHVKSIIHELIWFLTG"
BASE COUNT          885 a         1166 c         1252 g          827 t
ORIGIN      
        1 gccgcgattc aaggcgcggc tctgtcgggc gatcgcaagg atctgctgtt gctggacgtg
       61 acgccgctgt cgctgggtat cgaaaccatg ggcggtgtca tgaccaaaat gatcaagaag
      121 aacaccacta tcccaaccaa attcagccag gtattttcga cggctgatga caatcagccg
      181 gccgtgacca tcaaggtcta tcagggcgag cgtgaaatgg cggccggcaa caagggtctg
      241 ggtgaattca atctggaggg tattcctccc gcgccacgtg gcacgccgca gatcgaagtg
      301 accttcgaca tcgacgccaa cggcattctg cacgtcggcg ccaaggacaa ggccaccggc
      361 aaggaaaaca agatcaccat caaggccaac tccggtttga ctgaagagga aatcgaaaag
      421 atggtgcgcg acgccgaagc caatgccgaa gaagacaagc gcatgaagga actggccgaa
      481 acccgcaatc agggcgacgc gctggttcac tcgacgcgca agtcgctgac cgaatacggc
      541 gacaggctgg aaagcggtga gaagggtgcc atcgaagctg ccgtcaagga actggaagaa
      601 gtactaaagg gtaatgacaa ggcagccatc gatgccaaga ctgccgcagt gtccgtggcg
      661 gcgcaaaagc tgggcgagaa aatgtatgcc gatcagcagg cgcaggctgc ggccagcggg
      721 gcaggcgctg gtgcagccag tgcgggcgaa gcaccgaaac acgacgatgt ggtcgatgcc
      781 gatttcaagg aagtgaagta attccgtttg cgcatgggag gctccggcct ccactttcag
      841 gcgcggcatc cgtgatacag caatacgccg agccagacgg tcgcaagatc gtctctcggc
      901 tttttcatat cgttggtttg cggttgattc tcaagcttgc gcggagaagc aacgataaag
      961 tggttataaa acatggcaaa acgtgatttt tacgacattc tgggtctggc gaaaaatgcg
     1021 actgaggatg acatcaagaa ggcttatcgc aaactggcga tgaagtacca tcctgaccgc
     1081 aaccctgaca gcaagagcgc ggaagaaaag ttcaaggagg tcaaggaagc ctacgagata
     1141 ctgtccgatc cgcaaaagcg cgaggtctac gatcgttacg gtcatgccgg cgtggacccc
     1201 aatatgggtg gcggcgcggg cgcaggtggt ttcgccgatg ctttcggcga tatttttggc
     1261 gatattttcg gtggcggcgg tcgtggccgc cgctcggaat cgcaggtgta tcgcggcgcc
     1321 gacttgcgct acaacctgga aatcacgctt gagcaggccg ctcacggttt cgataccacc
     1381 attcgtgtgc cgtcctggga tgagtgcgat acctgtcacg gctccggtgc caagccgggg
     1441 acatcgccga ccacctgcgc tgcttgtggc ggtcatggtc aggtgcgcat gcagcagggc
     1501 ttcttcagca ttcagcagac ttgtccgaaa tgtcatggca gcggcaaaat cattcccgat
     1561 ccttgttcga cctgcgccgg cgccggccgc atcaagcgca acaagacctt ggaagtcaag
     1621 attccggaag gcatcgacga cggcatgcgc attcgttcgt ccggtaatgg tgaaccaggc
     1681 atgaacggcg gtcctccggg cgacctgtat gtggaaattc acatcaaacc gcacaaagtt
     1741 ttccggcgcg acggcgacga cctgcattgt gaaatgccga tttcgttcac cagggcggcg
     1801 ctgggcggcg acatcgaagc gcccactttg agcggcaagg ccacttttac gatccccgaa
     1861 ggcacgcaat cgggcaagat cttccgtctg cgcagcaagg gcatcaaggg ggtacgctca
     1921 ggctatgccg gcgatctgtt ctgccacgtc gtggtggaaa taccggtcaa gctgaccgag
     1981 cgccaaaaag agttgctgca cgagttcgag aaactgaccg tggaaggcgg cgccaagcat
     2041 aatccacagg ccaagacttg gatggacaag gtcaaggaat tcttcgagta agttttcatg
     2101 atgcagccgc aatgaaaaaa tcctgcattc agcatgcagg attcagactg ctgatgagcc
     2161 ccatgctttt cggaacacgg agttgtttat gcgcaggcga tgttggggcg gtttggcgta
     2221 atcttgctga gtaagtcggg agaacggcga tgaaacgcac cgatgacgtg ctgctcgtga
     2281 tcgatgtgca gaacgatttt atgcccggcg gcgcactcgc ggtatccgat ggcgacgtga
     2341 tcgtacccgt gatcaacgcg cttgccgcgc gcttcgatca ggtcgtgctg acgcaggact
     2401 ggcatccgcg cgaacacctg tcattcgcag cgaaccatgc gggtgcggtg ccgttctcga
     2461 gcgttgcgct cccgtacggc gagcaggtgc tgtggccggt acactgcgtg cagcagacca
     2521 gcggcgcgtc gtttcatcgc gaccttgacg tgcggagcgc gcgcggtgcg gtgatccgca
     2581 agggccacaa cgcgcaggtg gacagttact cggcgttcgt cgaagcagac cgcacgacgc
     2641 ataccgggct cgctgactat ttgcgcgagc ttggcgcgaa gcgcgtgtgg tgctgcggcc
     2701 tcgcgacaga tttctgcgtc gtgcggtccg cgctcgatgc gcgcgcggcg ggcttcgatg
     2761 ccgcggtgat cgacgatgcg tgccgtgcga tcgacctgtc cgggtcaatg gcgcgcgcat
     2821 gggaacagat gcaggcggcg ggcgtggtgc ttgtcacgtc cgatggcgcg cgcccgaaag
     2881 cataatgcac ttactcaatt cacgacagcg gctcatcgcc gatctctcga cttaaatcgt
     2941 gaacacgctc tcagcagtct gagtcctgca ttcaacattt tttgttcaag gccgggcagt
     3001 atttcaaaaa caatgttctg cagcaggaaa attttggttg cgcaccgcta tcacataggc
     3061 gcgcacggct tcctccacgc tggcggcgcc ttccatgaaa tttttcacga attttgcttg
     3121 atggccagca aacacgccca gcatgtcgtg catcaccagc acctgaccgg agcaatcaac
     3181 gccagcgccg atgccgatgg tcggtatatg ccgttgcgcg gtgatttcgg cacccagtgt
     3241 cgcgggaacc gcttcgagca acagcagcga tgctccggcc gtctgcaagg cggcggcgtc
     3301 cgcatgcagc agctcggcgc tttcccgcgt cttgccttgc actttatagc cgcccagctg
     3361 atgtactgat tgtggcatca ggcccagatg cgcccatacc ggaatgccgc gttcggtcag
     3421 caggcgcacc gtgtcagcca gccaggcgcc gccttccagt ttgaccatct gtgcgccggc
     3481 ctgcatcagt ttgacggcat tgtcgaatgc tgccggcggc gaaccgtagg tgccgaacgg
     3541 catgtcagcc gcgatcatca tcgtccggtt gccgcgtgct acgcaggcgg tgtgataggc
     3601 aatgtcatcg atggtcaccg gcagtgtcga ctggtggccc tggcatgtca tccccagcga
     3661 gtcgccgatg agtaccatgt cgacgccgca gcgctccatc agagcagcga agctggcgtc
     3721 gtaacaggtc agcacgctga tcttctcgcc tttgtcgcgc atggcttgca gcacaggtat
     3781 ggtcacgggt tgcttgcgcg ggttcggtgc atcttgcaga taagctgcca ttatttgttc
     3841 tcttcgattc aggaatacgg cttgccgcac ggtagcgcgc atggcgaaat ggagtgacag
     3901 tataatcttt cctgttccat tcgcctccca tcccatgcgc caatacctcg atttcatgcg
     3961 ccatgtctat gaacatggca ccgacaaaac cgatcgcaac ggcaccggca cccgttctgt
     4021 cttcggctat cagatgcgct tcgacctgca agacggtttt ccgctgctga ccaccaagaa
     4081 gctgcatgtc aagtcgatca tccatgaatt gatctggttt ctcaccggct
//
LOCUS       DASRDH010000094         9931 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_964761,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000094 DASRDH010000000
VERSION     DASRDH010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 9931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9931
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_964761"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..866)
                     /locus_tag="VFA14_02275"
     CDS             complement(<1..866)
                     /locus_tag="VFA14_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HZS80243.1"
                     /translation="MATSENNAALAASSRKRLHPIVRIALGTVGVLAALVALTVLALV
                     LVVTLAYPKLPDLGSLTDYHPKIPLRVFSSDGVLIGEFGVERRNLVHIKDIPTLMKKA
                     VLAIEDDRFYEHSGVDYQGIVRAAFSNLSGGGGGASTITQQVARNFFLTSNEPTFLQK
                     ATRKFFYEIPLAWKIEHNLTKDQILEIYMNQIYLGQRAYGFAAAAQIYFGKPLKELTI
                     AEAAMLAGLPKAPSAYNPVVNPKRAYARQQYILLRMRQLGYITDRQYFDAKDEQLKIK
                     GDSTDFGVHAEYV"
     gene            1138..2091
                     /gene="pilM"
                     /locus_tag="VFA14_02280"
     CDS             1138..2091
                     /gene="pilM"
                     /locus_tag="VFA14_02280"
                     /inference="COORDINATES: protein motif:HMM:NF022554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HZS80244.1"
                     /translation="MLSFPRLRLSRQNALAGLDIGRHAIRLVELSRDGGSLTLEAYGC
                     EPLPDGVVNDDGIDDLEHVITVARYLLEKCGSRTGKTVIGIPSGAVATHIFRIARLSS
                     GTEAQLETLAREHIAALLNYPAEDACIDFCRMGDAPAATAHIDVLVAATHRENVEDRV
                     AVVESLHCHTVIADIDSYAAHAALARSRGSDTATTALCQIDAHGMHLSLPVDGRIVQV
                     HDDANGTSDPMRVATAAARTLQTWQAMYPEHRVQVLVFSGIGATMHGLNEAFHQHTGI
                     SSSIADPFCGMSLGAAIDTVQLTRDAPACLVACGLALRHFN"
     gene            2099..2668
                     /locus_tag="VFA14_02285"
     CDS             2099..2668
                     /locus_tag="VFA14_02285"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HZS80245.1"
                     /translation="MLRFNLLPHRRLKRQQRWYLQLYAALATAVMSVLLADMLLNRQL
                     AQQSSLAQRQQKEHDRLDAQLRQLADMQRQLVKLEKQRQTIEALREQQNQPLQLLQTL
                     AHATPHSVQLQTLKLQAAHLILSGTAPDHQNIMQLLANLHRAGDMFALAELQEIRAIH
                     NRSHDFVIAAKRPAVSPTPADPGMEKNRQ"
     gene            2665..3624
                     /locus_tag="VFA14_02290"
     CDS             2665..3624
                     /locus_tag="VFA14_02290"
                     /inference="COORDINATES: protein motif:HMM:NF016254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HZS80246.1"
                     /translation="MRQQRHPGDWPRRYQALLCLAAFLAVLGIGWQFLLQPDIRRLDN
                     DRQEAQRRHIAAAQKARQAAALPPLQQRLKEATATLRVQQGQLSTPADMHTMLEHIAT
                     AAQEHGLQFEFTRPNKAAPFPDDAVQIGAIRLNGPYHALGHFAADLAALRYILVLSEL
                     QLSASEGGLVAMDAVLLHHHQPPQENHRIAIVPEPAFAPLAYEDGMDRSPFDPVGMMA
                     SLPADAPAARPKQRKAPQPLEKVPLASIRMVGTIAQHGRRAVLLQVGQHMHLAGVGDY
                     AGENFGMIRHISDHETELEELIPNADGVWTPRKTTLVLQDDKS"
     gene            3621..5657
                     /gene="pilQ"
                     /locus_tag="VFA14_02295"
     CDS             3621..5657
                     /gene="pilQ"
                     /locus_tag="VFA14_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HZS80247.1"
                     /translation="MKKQLRSLCAILAWLPILAPAHNTLVSIHADEQSGRTSIRIELS
                     APPDAIPVHFSTIDPPRIVLDLPAADNATGDHGRKLDLRILSAYRIAHTEERSRLIFS
                     LTQLAPYTVRTEGKTIVLDIASPWPAAVHQADRPRLQNIDFRRGAQAEGHIIIDLPGA
                     STLLNIRRQGRQLIAEFRDTTLPQAMQRYLDVSDFGTPVHSIRSTQEGADVRMLIDAE
                     GAWKHHAYQKDAQLVIEVRHENDIRTPPSYRGDKLSLHFQNVEVRAILHVIADFTGLN
                     IITSDSISGVLSLRLKDVPWDQALDIVLQARGLGMRRNGNVMWIAPHDELLARERLEL
                     EQHAQNMALEPMQTEVFQLNYQKAETFRKMFSISDGSSPANGLRHNNLLSRRGSAIVD
                     QRTNQLFVTDTPTVLANIRKLLDKVDIAARQVLIEARIVEADDSFSRHLGVRLGLSAR
                     NNHVAVGNTYASLGEPTDPKSVAPAAHADHHAINLPARAIGDATAGSIALSLFNAAAN
                     RVLDLELSALEADGKGRIVSSPRVVTADQQAALIEQGEEIPYQQATSSGATSTAFKKA
                     NLKLEVTPQITPDGNVILNVDINKDSRGIPTPGGLAINTKHVKTLVQVENGGTVVIGG
                     IYTQTESLAVNKVPLLGDIPVLGNLFKNTSRIDNKTELLIFLTPRIVQDHPFPR"
     gene            5798..7510
                     /gene="aroKB"
                     /locus_tag="VFA14_02300"
     CDS             5798..7510
                     /gene="aroKB"
                     /locus_tag="VFA14_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional shikimate kinase/3-dehydroquinate
                     synthase AroKB"
                     /protein_id="HZS80248.1"
                     /translation="MSGSIFLVGLMGTGKTTIGRALAKKLDKRFVDSDHEIELRTGAS
                     IPMIFEIEGEESFRKREADVIRELSAQKNIVLATGGGAVLRAETRACLKQHGTVIYLR
                     ADVNQILQRAGRDKNRPLLQTADPRKKLEELSRQREPYYQEVADFIIETSRPNMRLLV
                     QFIMSRLELMPEHTAVSTSTPASLNRLPAEPVMSVLPHAPITLQVDLEKRSYPITIGH
                     GLLSDSSLLTRHIAGKHAVIVTNDKVGPLYLAQVRKLLEDAGKQVTGIVLPDGEETKN
                     WTSLMKMFDVLLTEKCDRKTTMIALGGGVIGDLTGFAAATYMRGVPFVQIPTTLLAQA
                     DSSVGGKTGINHPLGKNMIGAFHQPQAVIADTATLHTLPARELSAGLAEVIKHGAIID
                     ISFFGWIETNIGKLMVRDNVALAYAIQHSCKIKADVVRQDEREGGLRAILNFGHTFGH
                     AIESGLGYGQWLHGEAVGCGMVMAADLSYRLGYIDATAKNRLTALVKAAGLPATAPDL
                     GLSRWLELMEIDKKNENGRTRFILLKPLGHSLVTDVPQNLLLQTLTACTPPLTPSLPC
                     STTD"
     gene            7538..8689
                     /locus_tag="VFA14_02305"
     CDS             7538..8689
                     /locus_tag="VFA14_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HZS80249.1"
                     /translation="MTQDTPPHLAPYAAHAANSAGRRFTEPAPGSRTEYQRDRDRIIH
                     STAFRRLEYKTQVFVNHEGDLFRTRLTHSIEVAQIARSCARNLHLNEDLVEAISLAHD
                     LGHTPFGHAGQDELNACMHNFGGFEHNLQSLRVVDKLEQHYGAFDGLNLMFETREGML
                     KHCSLTNARQLGTIGQRFIDKKQPSLEAQLANFADEIAYNNHDIDDGLRSGLLTIEQL
                     SEVDFFARHQREVEKIYPGITGRRLINETVRRMINTLIVDLLHTSQDNIAAIMPRSID
                     DVRNAPPLISFSASIREEAAILKRFLRQNLYRHYQVNRTTAKARRIIAELFHAFLSQP
                     ALLPPDYQVHTHTEDATRQQALQVSDYIAGMTDRYAMREHRRLFMMEEL"
     gene            complement(8725..9759)
                     /locus_tag="VFA14_02310"
     CDS             complement(8725..9759)
                     /locus_tag="VFA14_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007884215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HZS80250.1"
                     /translation="MYLRTFVASVVLAAASAGVFAQDKVINLYSARHYQADEALYNNF
                     TKATGIRINRVDADDAGILARLKSEGAASPADVILLVDAARLWRAENDGLFQPVKSGV
                     LESRIPVKLRGKDDGHGSQWFGFSMRARTIVYNKQTVKPEDVDSYEALADPKNKGKLC
                     TRSGSHPYNVSLFGALYEHVGEARTEAILKGMVANMARLPVGSDTDQIKAVASGECGV
                     AVSNSYYVARLMKSTKPEDIAVVSRIGVVWPNQKTYGVHVNIAGAGVAKYAPHKADAV
                     QFLEYLSSDEAQRYFAEGNNEWPVVKSLKTSNAALDKLGPFKAEVINVATIGRNQQKV
                     LQILDRVGYR"
BASE COUNT         2367 a         3137 c         2507 g         1920 t
ORIGIN      
        1 acatattccg catgcacacc gaaatccgtg ctgtcgccct tgatctttag ctgctcatcc
       61 ttcgcatcga agtactgccg atcagtgata tagccgagct ggcgcatgcg cagcagaatg
      121 tattgctggc gcgcgtaagc ccgcttggga ttgaccaccg ggttgtaggc cgatggcgct
      181 tttggcaaac cggccagcat cgctgcttcg gcgatggtca gctccttgag cggtttgccg
      241 aaataaatct gtgccgccgc tgcaaaaccg taagcacgtt gccctaggta aatctggttc
      301 atgtagatct caagaatctg atctttggtc agattatgct cgatcttcca ggccagcgga
      361 atctcataga agaatttgcg cgtagccttc tgcaggaaag tcggctcgtt actggtcagg
      421 aaaaaattgc gcgcgacctg ctgcgtgatc gtgctggcgc cgccaccgcc gcctgacaga
      481 ttggagaaag cagcacgcac gatgccctgg taatcgacgc cgctatgttc ataaaacctg
      541 tcgtcctcga tcgccagcac ggctttcttc atcaacgtgg gaatatcctt gatatgcacc
      601 aggttgcggc gctccacgcc gaattcgccg atcagcacgc catcggaaga aaacacgcgc
      661 aacggaatct tgggatgata gtcagtcaga gaaccgagat ccggcagttt gggatacgca
      721 agcgtgacta ccagcaccag cgccagtacc gtcagagcga ccagcgccgc aagcacgcct
      781 accgtaccca gcgctatgcg gacgatcgga tgcaaacgtt tcctggaaga tgccgcaagc
      841 gccgcgttat tttctgaggt ggccatgcat tgaaacagag ttggagaaag acaacccttc
      901 cattatagcg acttgcaagg agacgcaatt cagcagcaag aaatccgatc cgtatcggct
      961 atccgtgcag gcatgtcttg tccagacagt atttcagttc atccatttcc cgactaccgc
     1021 aggaaatttc tttacagaca ataaattctg ttgataggat acataggcct atcaaaaaga
     1081 taattttttt ctgtttgtgc ttccgatctc ctcccctcgg tttcctgaaa aacaatcgtg
     1141 ctgagctttc cgcgcttacg cttatcccgg cagaacgcac ttgccggcct cgacatcggt
     1201 cgccatgcaa tccgcctggt ggaactgtcc cgcgacggtg ggagcttgac gctggaagct
     1261 tacggctgcg agccattgcc cgacggggtc gtcaacgacg atggtatcga tgatctggaa
     1321 catgtcatca cagtcgcgcg ctatttgctg gaaaaatgcg gttcccgtac tggcaaaacc
     1381 gtcatcggta ttccctccgg cgccgtcgct acccacatct tccgcatcgc ccgactatcc
     1441 tccgggacag aagcacaact agaaacgctc gcaagagaac acatcgccgc tctgctgaac
     1501 tatcctgcag aagacgcctg catcgatttc tgccgcatgg gcgacgcgcc cgcagccact
     1561 gcacacatcg acgtgctggt cgctgccaca catagagaaa acgttgaaga ccgcgtcgcc
     1621 gttgtcgaat ccctgcattg ccataccgtc atcgccgata tcgacagtta cgccgcgcac
     1681 gctgccctgg cgcgatcgcg cgggtctgat actgccacga cagccttgtg ccagatcgat
     1741 gcgcatggca tgcatctgtc cttgcctgta gacggtcgca tagtgcaagt gcatgatgat
     1801 gccaacggca cgtccgatcc catgcgcgtc gcgacagcag cggcgcgcac attgcagacc
     1861 tggcaggcca tgtaccccga acatcgcgtg caagtactgg ttttctcggg aatcggcgcg
     1921 actatgcatg gattgaacga agctttccac cagcacaccg gcatctccag tagcatcgcc
     1981 gatccttttt gcggcatgtc gctgggcgct gcgatcgaca ccgtgcaatt gacgcgcgat
     2041 gcgccagcct gtttggtagc gtgcgggctg gcgctcaggc atttcaattg atacgcgaat
     2101 gctgagattc aatctgttgc cgcatcggcg gctgaaacga caacaacgct ggtacctgca
     2161 actgtatgct gcgctggcga ccgctgtcat gagcgtattg ctcgccgata tgctgctgaa
     2221 ccggcaactc gcacaacaaa gttcactggc gcaacgccaa caaaaagaac acgacagact
     2281 tgatgcacag ttacgccagc ttgctgatat gcagcgccaa ttagtcaagc tggaaaaaca
     2341 aagacaaacc atcgaggcat tgcgcgaaca acaaaaccag cccttgcaac tgctgcaaac
     2401 gctggcgcat gcaacaccgc acagcgtgca attgcagacg ctcaaactgc aggcggcaca
     2461 tctcattctg agcggaacgg ctcccgacca tcagaacatc atgcaattac tggcaaacct
     2521 gcatcgtgcc ggcgacatgt tcgccctagc cgaactacag gaaatccgtg caatacacaa
     2581 tcgcagtcac gacttcgtga ttgccgccaa acgtcccgcc gtctcgccga caccagctga
     2641 tcccggcatg gaaaaaaacc ggcaatgaga cagcaacgcc atcccggcga ctggccgagg
     2701 cgctaccagg cgctgctttg ccttgctgcc tttctggccg tgctcggcat cggctggcaa
     2761 ttcctcctgc aaccggacat acgccgtctc gacaacgaca gacaagaagc gcagcgccga
     2821 cacatcgccg ccgcgcaaaa agctaggcag gctgccgcac tgccgccgtt gcagcagcgc
     2881 ctgaaagaag ccacggcaac gttgcgggtg caacaagggc agctgtccac tcctgccgac
     2941 atgcatacca tgctcgaaca tatcgcgaca gccgcgcagg aacatggatt gcagtttgaa
     3001 ttcaccagac cgaacaaagc agcaccgttc cccgacgatg ccgtacagat cggcgccatc
     3061 cgactcaacg gtccctacca tgcgttgggc catttcgccg ctgatctggc cgctctccgg
     3121 tacatcctgg tgctgagcga gctgcaactc tcagccagtg aaggcggact ggtcgccatg
     3181 gatgccgtcc tgctgcacca tcaccagccg ccacaggaaa accaccgtat cgctatcgtt
     3241 cccgagcctg cattcgcccc tctcgcttac gaggacggta tggatcgctc gccattcgat
     3301 ccagtaggga tgatggcatc gctcccggcc gacgctcctg ctgcaagacc aaaacagcgt
     3361 aaagcgccgc aaccgctgga gaaagttccg ttggcctcga tacggatggt cggaacgatt
     3421 gcacagcacg gacgccgtgc ggttctgctg caagtcggcc aacacatgca tctggccggt
     3481 gtcggcgatt acgcgggaga aaatttcggc atgatccggc acatttccga ccacgaaact
     3541 gaactggaag aattgattcc caatgccgat ggcgtctgga cgccacgcaa aacgacgctg
     3601 gttttgcaag acgacaagtc atgaagaagc agcttcgatc attatgcgca atcctggcat
     3661 ggctgccgat actggcgccg gcgcacaaca cgcttgtttc catccacgcg gacgaacaga
     3721 gcggacgcac cagcatccgg attgaactca gcgcgccgcc ggatgccata ccggtacatt
     3781 tctccaccat cgatccgccg cgtatcgtgc tggatcttcc cgccgccgac aatgcaaccg
     3841 gtgaccatgg tcgcaaactc gacctgcgca ttttgagcgc ctaccgcatc gcgcataccg
     3901 aagaacgcag tcgtctcatt ttcagtctga cgcagcttgc gccttataca gtcaggacag
     3961 aaggcaaaac catcgtgctc gacatcgcct cgccttggcc ggcggcagta caccaagcgg
     4021 atcgtccacg actgcaaaac atcgacttcc ggcgcggcgc gcaagccgaa ggacacatca
     4081 tcatcgattt gccaggggcg tcaaccctgc tcaatatacg ccggcaaggc agacaactga
     4141 tcgccgagtt ccgcgatacg acgctgccgc aagccatgca acgatacctc gatgtcagcg
     4201 acttcggcac gccggtacac agcattcgca gcacgcagga aggcgccgac gtgcgcatgc
     4261 tcatcgatgc cgaaggcgcg tggaaacatc atgcctatca gaaggatgca caactcgtca
     4321 tagaagtgcg tcacgagaac gacatacgca caccgccaag ctatcgcggt gacaaactat
     4381 cgctacattt ccaaaatgtc gaagtacgcg ccatcttgca cgtcatcgcc gacttcaccg
     4441 gcctcaacat cattaccagc gatagcatca gcggcgtcct cagcctgcgt ctcaaggatg
     4501 tgccctggga ccaggcactg gacatcgtgc tgcaggcgcg cgggctgggc atgcgcagaa
     4561 acggcaacgt catgtggatc gcgccacacg acgaattgct ggccagggag cgactggaat
     4621 tggagcaaca tgcgcagaac atggcactgg aaccgatgca gaccgaagtc ttccagttga
     4681 actatcagaa ggctgaaaca ttccgcaaaa tgttcagcat cagcgacggc agcagccccg
     4741 ccaatggcct gcggcacaac aacctcctgt cgcgccgcgg cagcgccatc gtcgaccaac
     4801 gtaccaatca actcttcgtc actgacacgc cgacggtgct ggccaacatc cgcaaattgc
     4861 tcgacaaggt cgacatcgcc gcgcgccaag tgctgatcga agcgcgcatc gtcgaagcag
     4921 atgattcctt cagccgccat ctcggtgtgc gtctgggatt gtctgccagg aacaaccatg
     4981 tcgcggtcgg taacacctac gccagtctgg gtgaaccaac cgacccgaaa tccgtagcgc
     5041 cggccgccca tgctgaccat catgcgatca atctgccggc tcgcgccatc ggcgacgcta
     5101 ctgccggcag tattgctctg agcttgttca acgccgctgc caatcgcgtc ctcgatctcg
     5161 aattgtcagc gctggaagca gatggtaaag gcagaatcgt ttccagccca cgggtcgtga
     5221 ctgcggatca gcaggccgcg ctgatcgagc aaggcgagga aattccctac cagcaagcca
     5281 ccagtagtgg tgccacctcc accgcattca agaaagccaa tctcaaactg gaagtcaccc
     5341 cgcaaattac gcccgatggc aatgtcattc tgaatgtcga catcaacaag gatagccgcg
     5401 gtataccgac acctggcggc ctggcgatca acaccaagca cgtcaaaacc ctggtccaag
     5461 tggaaaacgg cggtaccgtg gtgatcggtg gcatttacac gcaaaccgaa tcgcttgccg
     5521 tgaacaaggt accgctgctc ggcgacattc cagtattggg caatcttttc aaaaacacga
     5581 gcagaatcga caacaaaaca gaactactga ttttcctgac accaagaatc gtgcaggacc
     5641 atccttttcc gcgttgaagc cgcgccaaag ccatcgtttt tccgatattt tcccgacaag
     5701 gcagcacaga cggtaaaatc gtctgctaca tcctgtcgcc atgaaaacac tcccgcgaca
     5761 ggttttccgg acaattctgc agcaaacaac ctggcatatg agtggaagca tttttcttgt
     5821 cggcttgatg ggcaccggca agacaaccat agggcgcgcc ctggcgaaaa aactggacaa
     5881 gcgcttcgtc gattccgatc acgagatcga gttgcgcacc ggagcctcca ttcccatgat
     5941 ctttgaaatc gaaggggagg aaagttttcg caagcgcgag gccgacgtca tccgtgaact
     6001 cagcgcccag aaaaacatcg tcctggccac tggtggtggc gctgttctgc gcgccgaaac
     6061 ccgcgcctgt ctaaagcagc acggcaccgt aatttacctg cgcgcggacg tcaaccagat
     6121 cctgcaacgc gccgggcgtg acaaaaaccg tccactgctg caaaccgccg atccgcgtaa
     6181 aaagctcgaa gaattgtcac gccagcgtga accttattat caagaggtag ctgacttcat
     6241 catcgaaacc agtcgaccga acatgcggct cctggtacag ttcatcatga gtcgactcga
     6301 gctgatgcct gaacacactg ctgtcagtac atcgacaccg gcatcgctca accgcctccc
     6361 tgcggaacca gtcatgagcg tgctgcccca tgctcccatc accctgcaag tcgacctgga
     6421 aaagcgcagc tatccgatca ccatcggcca tggcctattg tccgattcat cactgctgac
     6481 ccgccatata gcgggcaagc atgccgtcat cgtcaccaac gacaaagtcg gcccgctgta
     6541 tctggcacag gtacgcaaac tgctcgaaga cgccggcaaa caagtgaccg gaattgtcct
     6601 gcccgacggt gaagaaacaa aaaactggac cagcctgatg aagatgttcg atgtcctact
     6661 gacagaaaaa tgcgaccgca aaaccaccat gatcgcgctc ggcggcggcg tcatcggcga
     6721 tctgaccggc tttgccgcgg caacctacat gcgcggcgtg ccgttcgtac agatccccac
     6781 cacgctgttg gcgcaagccg attcttcggt cggcggcaag accggcatta accacccgct
     6841 cggcaaaaac atgatcggcg ccttccatca accgcaggca gtcatcgccg ataccgcaac
     6901 actgcacaca ctgccagcgc gcgagctgtc tgccggtctg gccgaagtca tcaagcacgg
     6961 cgccatcatc gacatctcct tcttcggctg gatcgaaacc aatatcggca aactgatggt
     7021 cagggacaat gtcgcactgg cctatgccat tcagcattcc tgcaaaatca aggccgacgt
     7081 cgtgcggcag gacgagcgcg aagggggcct gcgcgccatc ctcaacttcg gtcacacctt
     7141 cggccacgcc atcgaatccg gactcggcta tggccaatgg ctgcacggtg aagctgtcgg
     7201 ctgcggcatg gtgatggcgg cggatctatc gtatcgcctg ggctatatcg acgccaccgc
     7261 caaaaaccgc ctgacggccc tggtcaaggc cgccggcctg cctgccacag cgcccgacct
     7321 gggcctttcg cgttggctcg aactgatgga aatcgacaag aaaaacgaaa acggccggac
     7381 cagattcatt ctgcttaaac cgctgggaca ctcgctggtc accgacgtgc cgcagaacct
     7441 cctgttacaa accctgacag cctgcacccc ccccctgact ccatctctgc catgttccac
     7501 aaccgattga agagcattca tgaaatgagt ttctgacatg acccaagaca caccaccgca
     7561 cctcgcccct tacgccgcac acgcagcaaa ctcggcagga cggcgcttca cagaaccagc
     7621 gccgggttca cgcacagaat accagcgcga ccgtgatcgc atcattcact ccaccgcctt
     7681 ccgccgcctc gaatataaga ctcaggtctt cgtcaatcac gaaggcgacc tgttccgcac
     7741 gcgcctgacc cacagcatcg aggttgccca gatcgcccgc tcctgcgcgc gtaacctgca
     7801 tctcaacgaa gatctggtgg aagccatctc actggcgcat gacctcggcc atacgccgtt
     7861 cggtcatgcc ggccaggatg aactcaacgc ctgcatgcat aatttcggcg gcttcgaaca
     7921 caatctgcaa agcctgcgcg tcgtcgacaa actggaacag cactacggcg ctttcgacgg
     7981 cctgaacctg atgtttgaaa cgcgcgaagg catgctcaag cactgctcgc tgacgaatgc
     8041 acgccagctc ggcaccatcg gccagcgttt catcgataaa aaacagccct ctctcgaagc
     8101 ccagctagcc aacttcgccg acgaaatcgc ctataacaat cacgacatcg acgacggcct
     8161 gcgctccggc ctgttgacca tcgagcagct gagcgaagtc gatttcttcg cacgccatca
     8221 acgcgaagtg gaaaaaatct atcccggcat cactggtcgc cgcctcatca acgaaactgt
     8281 gcggcgcatg atcaatacgc tgatcgttga cctgctccac acctcgcagg acaatatcgc
     8341 cgccatcatg cctcgcagca tcgacgacgt gcgcaatgct ccaccactga tcagcttctc
     8401 cgcatcgata cgggaagaag ccgccatcct caagcgtttt ctgcgtcaga acttgtatcg
     8461 ccattaccag gtcaatcgca cgaccgccaa ggcgcgccgc atcatcgcgg aactgttcca
     8521 tgcctttctg tcccagccgg cgctactgcc gcctgattac caggtccaca cacacaccga
     8581 agatgcaaca cgccagcaag cgcttcaagt ctcagactac atcgccggca tgaccgatcg
     8641 ttacgccatg cgcgaacacc ggcgcctgtt catgatggaa gaactttaaa ttccaccact
     8701 gccgatgttc atctcagccg tcatttacct gtaaccgacg cgatcaagaa tctgcaacac
     8761 tttctgctgg ttgcggccga tcgtggcaac attgatcact tcggccttga acggccccag
     8821 tttatcgagc gcggcattgc tggtcttgag gctcttaacc acaggccatt cgttattgcc
     8881 ttcggcaaaa tagcgttgcg cctcatcact ggacaggtat tccaggaact gcaccgcatc
     8941 cgccttgtgc ggcgcatact ttgccacacc tgcgccggca atgttgacgt gtacgccata
     9001 ggtcttctga ttcggccaga ccacaccgat cctgctgacg acagcgatat cttccggctt
     9061 ggtcgacttc atcaaacgcg ccacgtaata ggaattggaa acggccacac cgcattcgcc
     9121 ggaagccacc gccttgatct ggtcggtatc gctgccaaca ggcaggcgcg ccatattggc
     9181 gaccataccc ttgagaatgg cttcagtcct agcttcaccg acatgctcat acagcgcgcc
     9241 gaacagagaa acgttatacg gatggctacc ggaacgggta cacagttttc ccttgttctt
     9301 cgggtcagcc aaagcctcat aagaatcgac atcctccggc ttcacggtct gcttgttgta
     9361 gacgatcgta cgagcgcgca tcgaaaaacc gaaccactgc gaaccatgac cgtcatcctt
     9421 gccacgcagc ttgacgggaa tacgtgactc cagtacgcca gacttcaccg gctggaacaa
     9481 accgtcgttc tcggcccgcc atagacgggc ggcatcgacc aacaagatca catcagccgg
     9541 acttgccgcg ccttcgcttt tgaggcgcgc caggatgcca gcatcatcgg cgtcgacgcg
     9601 gttgatacgg ataccggtcg ccttggtgaa attgttatac aaggcttcat cggcctgata
     9661 atgacgcgca gaataaagat tgatcacctt gtcctgcgca aacacgcctg ccgaagcagc
     9721 cgccaatacc acacttgcaa caaaagtacg caaatacatg agttacttcc ttccgctatt
     9781 ggaccaaacc acacgatggc aaaggcaaac ggaatatagc acaaatgaga ataatttctt
     9841 tttattgtac caagtgcttt ctcacttccc gctcaactca ggcgctatag aacctttccc
     9901 attatcggga cagttaccac gcgtacttca c
//
LOCUS       DASRDH010000095         2095 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1352146,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000095 DASRDH010000000
VERSION     DASRDH010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1352146"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /locus_tag="VFA14_02315"
     CDS             complement(<1..642)
                     /locus_tag="VFA14_02315"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006029351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HZS80251.1"
                     /translation="MGIAIRKLDSTEAGFREKLFAVLAFEAGEDAAIDRAAATILADV
                     KARGDAAVLAYTNRFDRLGAANVAALEIGQDELKKALAGLPAARRDALQTAAGRVRVY
                     HERQKQECGSGGFLYTEADGTMLGQKVTPLDRVGIYVPGGKAAYPSSVLMNAIPARVA
                     GVQEVIMVVPTPDGVRNPLVLAAASLAGVTRVFTIGGAQAIAALAFGTQTVPAV"
     gene            complement(673..1353)
                     /gene="hisG"
                     /locus_tag="VFA14_02320"
     CDS             complement(673..1353)
                     /gene="hisG"
                     /locus_tag="VFA14_02320"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017231788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HZS80252.1"
                     /translation="MTQQPSRQQHRQLTLALSKGRIFEETLPLLKAAGIEVTGDRADS
                     RKLILPTSDPAVRVIIVRASDVPTYVQYGAADFGVAGKDVLLEHGGEGLYQPIDLNIA
                     RCRLSVAVRDGFDYASAVRQGARLRVVTKYVRTARAHFAAKGVHVDLIRLYGSMELGP
                     LVGLSDAIVDLVSTGGTLRANRLVEVEHIMEISSRLVVNQAALKLKRERLQAILEAFD
                     KASRANHI"
     gene            complement(1370..>2095)
                     /locus_tag="VFA14_02325"
     CDS             complement(1370..>2095)
                     /locus_tag="VFA14_02325"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HZS80253.1"
                     /translation="ADGETVLENAAREPEVADLAHLLVAMGAKIEGIGTDRLVIQGVD
                     VLHGATHSVIADRIETGTFLCAVAAAGGDVTLKNTRGNLLDAVLDKLREAGAILTSGA
                     DWVRVQMARRPMAVSFRTTEYPGFPTDMQAQFMALNCLAEGTSHITETIFENRFMHVQ
                     ELNRLGASIDIEGNTAIVKGVEKFVGAPVMATDLRASASLVIAGLAAQGETLIERIYH
                     LDRGYDRMENKLSSIGAWIERVK"
BASE COUNT          408 a          657 c          620 g          410 t
ORIGIN      
        1 cacagcgggc acggtctgcg tgccgaacgc aagcgcggct atcgcctgcg cgccgccgat
       61 cgtgaatacg cgcgtgacgc ccgcgagact cgccgcagcg agcacaagcg gattgcgcac
      121 gccgtccggc gtcggcacga ccatgatgac ttcctgcaca ccggcaaccc tggccggaat
      181 cgcattcatc agtacggatg aaggataagc ggctttgccg cccggcacat aaatgccgac
      241 acggtccagc ggcgtgacct tttgaccaag catggtgcca tcggcttcgg tataaaggaa
      301 gccgccggaa ccacattcct gtttctggcg ttcatggtag acgcgcacgc gcccggcggc
      361 agtctgcaag gcatcgcgac gcgcagcagg caaaccggcc aatgccttct tcagttcgtc
      421 ctgaccgatt tccaaggccg cgacgttcgc cgcgcccaga cggtcgaaac ggttggtgta
      481 cgccagcacg gcggcatcgc cacgcgcctt gacgtccgcc agaatggtag cggcggcgcg
      541 atcgatagca gcatcttcac cggcttcgaa cgccagcacg gcaaacagct tctccctgaa
      601 gccggcttcg gtagagtcga gttttctgat agcgataccc atgatgtgcc tttgctgagg
      661 tcggtgtccg gttcagatgt gattagcccg cgaggcttta tcaaatgctt ccagaatcgc
      721 ttgcaggcgc tcgcgcttga gtttcagcgc tgcctggttg accaccagac gcgaggaaat
      781 ttccatgata tgttcgacct cgaccagcct gttggcgcgc agtgtgccgc cggtgctgac
      841 gagatcgacg atggcatcgg acaggccaac cagcggaccg agttccatcg aaccgtacag
      901 cctgatcaaa tcgacatgga cgcccttggc ggcgaagtgc gcgcgcgcag tcctgacgta
      961 cttggtgacc acacgcaggc gcgcgccctg acgcactgcg ctggcgtagt cgaaaccatc
     1021 ccgtaccgcg accgacaggc ggcacctggc aatgttcagg tcgatcggtt ggtacaagcc
     1081 ttcgccgcca tgttccagca gcacatcctt gccagccaca ccgaaatcgg cagcgccgta
     1141 ttgcacgtaa gtcggtacgt ccgaagcgcg cacgatgatc acgcgcacgg caggatcact
     1201 ggtcggcaga atcagtttgc gcgagtcggc ccgatctccg gtaacctcga tgccggcagc
     1261 tttgagcagc ggcagcgttt cttcgaagat acgccctttg gacaatgcca aggtgagctg
     1321 tcgatgctgc tgtcgggatg gttgctgagt cattttccgc tctggtaaat catttgacgc
     1381 gttcgatcca ggcgccgatc gacgacagtt tgttttccat acggtcgtaa ccacgatcga
     1441 gatgatagat gcgctcgatc agggtttcgc cttgcgccgc cagaccggcg attaccagcg
     1501 aagccgatgc gcgcaggtcg gtcgccatca ccggcgcgcc gacgaatttt tccacgcctt
     1561 tgacaatggc agtattgcct tcgatgtcga tggacgcacc cagacgattc aattcctgca
     1621 cgtgcatgaa acggttttca aagatcgttt cggtgatgtg gctggtgcct tcggcgagac
     1681 agttcagcgc catgaattgc gcctgcatat cggtcggaaa gcccggatat tcggtagtgc
     1741 ggaagctgac cgccatggga cggcgcgcca tttgcactcg tacccagtca gcgccggatg
     1801 tcaggatggc accggcttca cgcagcttgt ccagtacggc gtcgagcaga ttgccgcgcg
     1861 tgttcttcag cgtgacatca ccgcccgcgg ccgcgacggc gcacaggaaa gtaccggttt
     1921 cgatacggtc ggcgatcacg ctatgcgtcg cgccatgcag cacatcgacg ccctgaatca
     1981 ccagacggtc ggtgcctatg ccttcgatct tcgcacccat cgccaccagc agatgcgcca
     2041 ggtcggcgac ttcgggttcg cgcgcggcat tctccagcac agtttcacca tcggc
//
LOCUS       DASRDH010000096         2325 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_265500,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000096 DASRDH010000000
VERSION     DASRDH010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265500"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="VFA14_02330"
     CDS             <1..277
                     /locus_tag="VFA14_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS80254.1"
                     /translation="GGLGVSNQELISCLAEISGVPMTLGHPVTEFDKKFERAIKLNMP
                     FANTEWQFVRRGIDHVLGCDAAPEILTSADISRLANWYLDEMSTVCR"
     gene            complement(335..1294)
                     /locus_tag="VFA14_02335"
     CDS             complement(335..1294)
                     /locus_tag="VFA14_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008117337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZS80255.1"
                     /translation="MKLLVTGATGFVGKALITALAVDAQYMLRACVRRKNVAFPDNVD
                     VVMVSDLTVGTDWTAALAGIDTVVHAAARVHVMDDRAIDRLAQYRRVNTAATLRLAQQ
                     AADAGVRRFVFISSIKVNGEATPLGRPYTAHDTPQPADPYGISKHEAEQGLLALAGRT
                     GLELVIIRPVLVYGPGVQANFLSMMRWLDKGVLLPLGAIKSNRRSLVALDNLVDLIIV
                     CLRHPAAVNQVFLVSDGEDVSTTVLLQRTARALQRPACLVPVPVSWLTVAAGLLEKSE
                     LAQRLCGTLQVDITKTTELLQWRPPIGLDEALRKTAAALHMRQ"
     gene            complement(1309..>2325)
                     /locus_tag="VFA14_02340"
     CDS             complement(1309..>2325)
                     /locus_tag="VFA14_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HZS80256.1"
                     /translation="ELDFFGRVRNLKDAALSIYLATEEARQSAQISLVAQVAQTYLSE
                     RAYTEQLKLAQQTLESRRTNLELAKKRFDVGASSALDLRLAETLVQSARTAAATLSRQ
                     RAQAENALTLLVGKKIDELPPGQDLVLQGIVTDIPAGLPSDLLMRRPDIRAAEQKLLA
                     ANANIGAARAAFFPRISLTGLFGSASSTLDGLFESGSGTWSFGPQLSLPIFDFGRTSA
                     NLDLAEVRKSQAIVTYEKAIQTAFREVADALAARGTLDEQIDAQEAFLKAQQERLNLT
                     DLRYRNGIASSLDQLDAQRDLFSAQQALIQARQLRLNNAIDLYRALGGGLHETTVSAS
                     TNGK"
BASE COUNT          489 a          673 c          665 g          498 t
ORIGIN      
        1 cggcggtctg ggagtctcga atcaagaact catatcgtgc cttgctgaaa tcagtggtgt
       61 gccaatgaca ttaggccatc cagtcacaga attcgacaaa aaattcgaac gcgcaatcaa
      121 attaaatatg ccatttgcaa ataccgaatg gcagtttgtg cgccgtggga tcgaccacgt
      181 actaggctgc gacgcagcac ccgaaattct tacgagcgca gacatttcac gtcttgccaa
      241 ttggtacttg gatgagatgt ccactgtttg cagataatag ctgttatcaa tacgcattcg
      301 cgccagtgcc gtatgagttg agtggcaggt tttattactg acgcatgtgc aacgcagccg
      361 cagttttcct cagagcctca tcgaggccga ttggcggacg ccattgcagc agttcagtcg
      421 ttttcgtgat atctacctgc aacgtgccgc acaggcgttg agcaagctcg ctcttttcca
      481 gaagcccggc tgccacggtc aaccagctca ctggaacggg cacaagacag gcagggcgct
      541 gcaatgctct ggcggtgcgt tgcagcagca cggttgtcga tacatcttct ccatcactga
      601 ccagaaatac ctgattgact gcagcagggt gacgcaggca gacgatgatc agatccacca
      661 gattatccag cgcaaccagg ctgcggcgat tgcttttgat agcgccgagc ggcaacagaa
      721 cgcctttatc cagccagcgc atcatgctaa gaaaattggc ctggacgccg ggtccatata
      781 ccagcacagg ccggatgatg acaagttcca gtccggtcct cccagccagc gccaataaac
      841 cctgttccgc ttcatgtttc gatatgccat aaggatcggc cggctgcggc gtgtcgtgtg
      901 ccgtataggg ccgacccaga ggcgttgctt cgccattgac cttgatcgag ctgatgaaca
      961 cgaaccggcg cacgcccgca tcagcggcct gttgcgcgag gcgtaaggta gcggcagtat
     1021 tcacgcgacg gtattgtgcc agtcgatcga tggctcgatc gtccatcacg tgtacacgcg
     1081 ctgcggcatg cacgacagta tcaatgcctg ccagggcggc agtccaatcc gtgcctaccg
     1141 tcaggtcgga gaccatcacc acatcgacgt tgtcggggaa tgcgacattt ttacggcgca
     1201 cgcatgcgcg cagcatatat tgcgcgtcaa ccgcgagcgc cgtgatcaat gctttgccga
     1261 cgaagccagt ggcgccggtg accagtaatt tcatgcgcag gtgaaatgtt atttgccgtt
     1321 tgtcgatgcg ctcactgtcg tttcatgcaa tccgcctccc agtgcgcgat acagatcgat
     1381 cgcattattc agacgcaact gccgcgcctg aatcagcgcc tgctgcgccg agaacagatc
     1441 gcgctgcgca tcgagttgat cgagcgaaga cgcgatgccg ttccggtagc gcaagtcggt
     1501 cagattcaga cgttcctgct gcgctttcag gaaggcctcc tgcgcatcga tctgctcgtc
     1561 caatgtgccg cgcgccgcca gcgcatcggc gacttcgcgg aaggctgtct gtatggcttt
     1621 ttcgtaagtc acgatggctt ggctcttgcg cacttccgcc agatccagat tggcgctggt
     1681 gcgaccgaag tcgaaaatcg gcaggctcag ttgcggaccg aaagaccatg tacccgaacc
     1741 ggattcaaac aagccgtcca gagtgctgct ggcgctgccg aacaaacctg tcaggctgat
     1801 tctcggaaaa aacgccgcac gcgccgcacc gatgttggcg ttggcggcaa gcagtttctg
     1861 ttctgccgca cggatatcgg gacggcgcat gagcaggtcc gacggcaagc cggcgggaat
     1921 gtcggtgacg atgccttgca gcaccagatc ctggccgggg ggcaactcat cgattttttt
     1981 accaaccagc agcgtcaagg cattttccgc ctgagcgcgc tgacgcgaca gcgttgccgc
     2041 tgcggtacgc gcggattgca ccagcgtttc ggccaggcgc agatccagcg ccgaagaagc
     2101 gccaacgtcg aaacgttttt tcgccagttc gaggttggtc cggcggcttt ccagcgtctg
     2161 ttgcgccagc tttagttgtt cggtgtacgc gcgctcgctc agataggtct gggcgacttg
     2221 cgccaccagg ctgatttgcg ccgactgcct ggcttcttcg gtcgccagat agatcgatag
     2281 cgcggcatcc ttgaggttgc gcacgcggcc aaagaagtcc agttc
//
LOCUS       DASRDH010000097         5308 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_788464,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000097 DASRDH010000000
VERSION     DASRDH010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 5308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5308
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_788464"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..952)
                     /locus_tag="VFA14_02345"
     CDS             complement(<1..952)
                     /locus_tag="VFA14_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007882719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HZS80257.1"
                     /translation="MNTATETLNPALGFDKLTPFIARLLTVMSIAFTLLPGDFNWDHA
                     HQRSNFAEGSLLFQIKFGSLFLIGAWLAWRNRYWTLRLARRLNPLLIAMTLYCLSSIL
                     WSPYPIVTLKRSAQLIGLILVGIAISAPAVGKHQLIRSILYTLMTLMAFSLVVALTLP
                     HIGVDHELGGAWRGVLLQKNTLGAISGLCVLFWIKESRSNAFPRPICWFGILFGLFML
                     IMSKSSTSMLVTMLGAGTYLLARRRYLSVEFGIRRLLLILAVIILCGLHAFYVMQGRL
                     PEWDEWFAPLGALLNKSADLTGRTRIWHMTLLEIQQHPIFG"
     gene            complement(936..1862)
                     /locus_tag="VFA14_02350"
     CDS             complement(936..1862)
                     /locus_tag="VFA14_02350"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZS80258.1"
                     /translation="MSTTIDISICICTFRRPALLERLLMALTEQSCDGLRVEVVVVDN
                     DPAGSAADVLRDWQSRMTMPLHARHETTPNIAQARNTAVHTAQGEWVLFIDDDETPGT
                     DWIHQLVNTQRRYDADAVFAPVLPRYRDGTPDWIRAGDFFNRRRFATGSLITAKDART
                     GNVLIRRSKLMTMPGPFDTAFGRTGAEDTMLFRDMLAHGASFVWCDEAAVSEEVPAQR
                     ANLNWLLRRSYRLGQTYMLTEIARLDGKQRFLRAARLGARALLQLPVAAGAALLALPF
                     SRIASIRWLRTMLAQCGKLSALMGHRYHEYGN"
     gene            complement(1859..3175)
                     /locus_tag="VFA14_02355"
     CDS             complement(1859..3175)
                     /locus_tag="VFA14_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007882717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS80259.1"
                     /translation="MAPSTQMAERPATEVSDAGSSRLLTHSLASLIDQILLSGLNFAL
                     GLVLIRLATKESYGVYAQLYAAGIFAAMVIEALITNPLTTIAPSKSPDERTRLATLLL
                     RFQWQLSIVLALLLGVGSAIITGLADIDAHPVLLGIAFAVYLFTNAQREYHRSIGFIE
                     ARPRRVLQIDIAYVMTVAIGIGILLQIEYLTVPAIFSVMGLANIISMRRGRTWQTRVS
                     AHAAAASMTYRAAVAEIMQRGRWALPGALLAWITNYSYLYIIAAALGAAAAAELHASR
                     LLLMPVSLSALAWSRVARPMVSRLFAARDWKHLDRLAWTSLAGMEIITVLYIIALWLM
                     LPWLGMHVLGAKYQGLEPMVLGWGGYFVIHAARWIGSSLLISKDQYRLLLGSGIIGLI
                     VMLVAASVCIPLYGAWGAILTLIIVEVANLLLTWAIFLPMLRRSNT"
     gene            complement(3130..4074)
                     /locus_tag="VFA14_02360"
     CDS             complement(3130..4074)
                     /locus_tag="VFA14_02360"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007882716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HZS80260.1"
                     /translation="MTTATQASGAADRIGACFLAAGLLTEDQVAHVVELQNVVSIRFG
                     EAAIHLGLLTEQNVQEMLSRQFNYATALAPVAGLDNSLGIALAPFGQEAEAIRRIRAE
                     LSMRFPDQEKIAIAIVSPGDGEGKSYLAASLALAFSQMEKRTLLIDADMRFPVQHLLF
                     NLENKTGLSTMLAGRTAISSGILAQGFPYLRILNAGPRPPNPLEILLRPTLGKLMQQL
                     TDDFDTFIIDTPSAQTSSDAQIIARQVGTCLLVTRQHHSRLDQLRRTQTQMQTAGAHI
                     IGVVYNEFGEQDALDQLEGNGRKLWRRLRKWLSGPQRK"
     gene            complement(4088..>5308)
                     /locus_tag="VFA14_02365"
     CDS             complement(4088..>5308)
                     /locus_tag="VFA14_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GumC family protein"
                     /protein_id="HZS80261.1"
                     /translation="FLDYKGNDPISGRLFSPILDESYIQTQLNMLKSRALAEKVIDTL
                     DLERAPSYQESVERHGRARARSLLIQHINDNTRVITHRSSHVIEVEYAAATPGQARAL
                     TNAIVRAYIDLNQQISSDAARARREQYSVQLEHLRKEADAIQKKLTQYQQEVGILDPD
                     EHNDLQSRQLGNLMASLIDVQNRQQEAQARKNVTDSLLRNGVRIDELPEIAQLPNIND
                     LKSKLSDVNKRLADIQEVLGPRHPKTLALISEHDGIDARITREARASLKAQQIDTRRL
                     TMQEQALKQQVDAQHARLLKQKQHRDTIISYQRQLASVEHIYAAALAKYDEILMASNI
                     DTFGLTVLHVAETPGVHSRPLLLQNIAASIPVGLALGFCLALLCELSQRRVRCEDDLV
                     RGLPLPLIGHIGIR"
BASE COUNT         1033 a         1475 c         1736 g         1064 t
ORIGIN      
        1 tgccgaatat tgggtgctgc tgtatttcca gcagtgtcat atgccagatg cgggtgcggc
       61 cggtgaggtc ggcgcttttg ttgagtagcg cgccgagtgg ggcgaaccat tcgtcccatt
      121 caggcaggcg gccttgcatg acataaaagg catgtaaacc gcacaggatg atgacggcga
      181 ggatcagcag cagtcgccgg ataccgaact cgacgctcag atagcggcgc cgcgccagca
      241 ggtaggtgcc agcgcccagc atcgtcacca gcatgctggt gctgcttttg gacatgatca
      301 gcatgaacag gccaaacagg atgccgaacc agcagatcgg gcgcgggaaa gcattactcc
      361 ggctttcctt gatccagaac agcacgcaca ggccggaaat cgcgcccagc gtgttttttt
      421 gcagcagcac gccgcgccag gcgccgccga gttcgtgatc gacgccgatg tgcggcagcg
      481 tcagcgcgac caccagggaa aacgccatca gcgtcatcag tgtatacagg atgctgcgga
      541 tcaattgatg cttgccgaca gctggcgccg agatcgcgat gccgaccagt atcagtccga
      601 tcagctgggc actgcgtttg agtgtgacga tcggataggg agaccacagg atgctgctca
      661 ggcaatacag ggtcatggcg atcagcagcg gattgaggcg ccgcgccagg cgcagggtcc
      721 agtaccggtt gcgccaggcc agccaggcgc cgatcaggaa cagcgatccg aacttgatct
      781 ggaacagcaa cgagccttcg gcaaagttgc tgcgctgatg ggcatggtcc caattgaagt
      841 cgcctggcag caacgtgaag gcgatgctca tgacggtcag caggcgcgcg atgaacggag
      901 tgagcttgtc gaaccccaat gccgggttta atgtttcagt tgccgtattc atgataacga
      961 tggcccatca gcgcgctcaa tttgccgcat tgcgccagca tggtgcgcag ccagcggatc
     1021 gatgcgatgc gggaaaaagg gagcgccagc aatgctgcgc cggcggcgac cggcaactgg
     1081 agcaacgcgc gcgcgccgag tcgggcggcg cgcaggaaac gctgtttgcc gtcaagccgg
     1141 gcgatttccg tgagcatata agtctgtccc agacgatagg agcggcgcag cagccagttg
     1201 agattggcgc gctgcgccgg tacttcctca ctcacggcgg cttcgtcgca ccagacgaag
     1261 ctggcgccgt gcgccagcat gtcgcgaaac agcatggtat cttccgcgcc ggtacggcca
     1321 aaggcggtgt cgaacggccc tggcatggtc atcagcttgc tgcgccggat cagcacgttg
     1381 ccggtacgtg catctttggc cgtgatgagg ctgccggtag cgaagcgccg gcgattgaaa
     1441 aaatcaccgg cgcgtatcca gtccggcgta ccgtcgcggt agcgcggcag caccggcgcg
     1501 aatacggcat cggcatcgta gcgacgctgg gtgttgacca gctggtgtat ccagtctgta
     1561 ccgggcgttt catcgtcgtc gatgaacagc acccattcgc cctgcgccgt atggacagcg
     1621 gtattgcgtg cctgagcgat attgggtgtg gtttcgtggc gtgcatgcaa gggcatggtc
     1681 atacggcttt gccagtcgcg caggacatcc gctgccgaac cggccggatc gttgtcgacg
     1741 acgacgactt ccacgcgcag gccgtcgcag gactgttcgg tcaatgccat gagtaaacgt
     1801 tccagtagcg caggacggcg gaatgtacaa atgcagatgc tgatgtcgat ggttgtgctc
     1861 atgtgttgct gcgccttagc ataggcagga agatagccca ggttagcagc aggttggcga
     1921 cttccacgat gatcagcgtc aggatggcgc cccaggcgcc gtacaaaggg atacagacgg
     1981 atgccgcgac cagcatgacg atcaggccaa tgatgccgct gcctagcagc aggcggtact
     2041 ggtccttgct gatcagcagg gatgaaccga tccagcgcgc tgcgtgaatg acgaagtagc
     2101 cgccccagcc cagtaccatc ggttccaggc cctgatattt ggcgcccagc acatgcattc
     2161 ccagccaggg cagcatcagc cacaaggcga tgatatagag gacggtgatg atttccatgc
     2221 ctgccagcga ggtccatgcc aggcggtcga gatgtttcca gtcacgcgcg gcaaacagac
     2281 ggctcaccat tggccgcgcg acccgcgacc aggccagtgc tgacagggaa accggcatca
     2341 gcagcaggcg tgaagcatgc agctcagccg ctgccgccgc acccagcgcg gcggcgatga
     2401 tatagaggta gctgtagttg gtgatccacg ccaacagggc gccaggcagc gcccagcggc
     2461 cgcgctgcat gatttccgcg acggcggcgc ggtatgtcat cgatgcggcc gcagcgtgcg
     2521 cagatacgcg cgtctgccag gtgcggccgc gccgcatgct gatgatattg gccaatccca
     2581 tgacagaaaa aatcgccggt acggtgagat actcgatctg taacagaatg ccgatgccga
     2641 tcgctaccgt catcacatag gcgatatcga tttgcagtac gcgacgcgga cgcgcttcga
     2701 tgaagccgat gctgcgatgg tattcacgct gggcgttggt gaaaagatag acggcgaagg
     2761 caatgccgag caggaccggg tgtgcatcga tgtcggcgag gccggtgatg atcgcgctgc
     2821 cgacgcccaa cagcagcgcc aggacgatgc tcagttgcca ttgaaagcgc agcagcaggg
     2881 tagccagccg cgttcgctcg tccggtgatt tgctgggagc gatggtggtc agagggttgg
     2941 tgatcagcgc ttcgattacc atggcagcga agatgccggc agcatacagt tgtgcgtaga
     3001 cgccgtaaga ctctttggtg gccagacgta tcaataccag acccagcgcg aagttcaaac
     3061 cgctcaacag aatctggtcg atgagcgagg ccagcgagtg cgtcagcagc ctgctgctgc
     3121 cggcatcgct tacttccgtt gcgggccgct cagccatttg cgtagacggc gccatagttt
     3181 cctgccattg ccctcgagct ggtccagcgc atcctgttcc ccgaattcgt tataaaccac
     3241 gccgatgatg tgcgcgccgg cggtctgcat ttgtgtctgt gtccggcgca gctgatccag
     3301 gcgggagtgg tgctggcgcg tcaccagcag gcatgtgccg acctgccggg caatgatttg
     3361 cgcatccgat gaagtttgcg ccgatggtgt atcgatgatg aaggtgtcga agtcatcggt
     3421 caactgttgc atcagcttgc cgagagtggg ccggagtaaa atttccagcg ggttgggcgg
     3481 tcgaggaccg gcattcagga tgcgcaggta cggaaaacct tgcgccagga tgccgctaga
     3541 aatggcagtg cgccccgcca gcatggtcga caagccggtt ttgttttcaa ggttgaacag
     3601 cagatgctgt acgggaaaac gcatgtcggc gtcgatcagg agtgtgcgtt tttccatttg
     3661 cgaaaacgcc agcgccagac tcgcggccag gtagctcttg ccttcgccat caccgggact
     3721 gacgatggcg atagcgattt tttcttgatc ggggaagcgc atcgacagtt cagcgcggat
     3781 ccggcggatc gcttcggctt cctgaccgaa cggcgccagc gcgataccca gcgagttgtc
     3841 caggccggcg accggcgcca gcgcggtggc atagttgaac tgtctggaca gcatttcctg
     3901 cacgttctgc tcggtgagca gaccgaggtg tatggcagct tcgccgaagc ggatgctgac
     3961 gacgttttgc agctcgacga catgcgcgac ctggtcttcg gtcagcaggc cggccgccag
     4021 gaagcaggcg cctattctgt cggcggcgcc ggatgcttgg gttgccgttg tcatggtcat
     4081 ggtagtttca gcggatcccg atgtggccga tcagcgggag agggaggccc cgtaccagat
     4141 catcttcgca gcgcacgcgg cgttgactga gttcgcacag cagtgcaaga cagaaaccca
     4201 gcgccaggcc gaccgggatg ctggcggcaa tgttttgcag cagcagcggc ctggagtgga
     4261 cgccgggcgt ttcggcgacg tgcagcaccg tcaggccgaa ggtatcgata ttgctggcca
     4321 tcaggatttc atcatatttc gccagcgcgg cggcgtagat atgttcgacg ctggcgagct
     4381 gacgttgata agagataatg gtgtcgcgat gctgcttttg cttcagcagt ctggcgtgct
     4441 gggcatcgac ttgctgtttc agggcttgtt cctgcatggt caggcggcgc gtatcgattt
     4501 gttgggcttt tagcgaagcg cgcgcttcgc gcgtaatgcg cgcatcgata ccgtcatgtt
     4561 cgctgatgag tgccagggtt ttcggatggc gcggaccaag cacctcctgg atgtcggcca
     4621 gacgcttgtt gacgtcactc agtttggact tcaggtcgtt gatatttgga agctgcgcga
     4681 tttcaggcag ttcgtcgatg cgcacgccgt tgcgcagcag gctgtcggtg acattcttgc
     4741 gcgcctgtgc ttcctgctgc cggttctgga cgtcgatcag ggaagccatc aggttgccaa
     4801 gctggcgcga ctgcaaatcg ttgtgttcgt ccgggtcgag gatgccgact tcctgctggt
     4861 attgagtcag ttttttctgg atcgcatcgg cctccttgcg caaatgctcg agctggacgc
     4921 tatactgttc gcggcgcgcc cgggccgcgt ccgaggatat ctgctggttc agatcgatat
     4981 aggcgcgcac gatggcattg gtcagagcgc gtgcctgccc gggtgtggcg gcggcatatt
     5041 cgacttcgat cacgtggctg gagcggtgtg ttatcaccct ggtgttatcg ttgatgtgct
     5101 ggatcaatag tgaacgcgcg cgcgcccggc cgtgacgctc gacggattcc tggtaggagg
     5161 gcgcgcgctc caggtcgagc gtatcgatga ctttttcagc gagcgcccgg ctcttgagca
     5221 tattgagctg ggtttgtata tagctctcgt cgagtatcgg cgagaacagg cggccgctga
     5281 tcggatcgtt gcctttgtag tcgagaaa
//
LOCUS       DASRDH010000098         2001 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_597468,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000098 DASRDH010000000
VERSION     DASRDH010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_597468"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..932
                     /locus_tag="VFA14_02370"
     CDS             <1..932
                     /locus_tag="VFA14_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HZS80262.1"
                     /translation="KIIIMTDADVDGAHIRTLLLTLFYRQMPQLVERGHIYIAQPPLY
                     KVKAGKDERYLKDDVEEMHYMMQIALNDAELVPSEGAIPISGAPLIELVRQYNVANAI
                     IMRLARVIDDAALSAIMTGVTLQLNSAAEAAQSAAALKAAINVLDIDVIAYSDELTDR
                     HALRILRSYHGNIKVTAIDTDFVDSPDYRVLSNAAAVFKGLIGRGALIRRGAGDKMKE
                     NAIADFHQAMAWLRAEAERSVSKQRYKGLGEMNPEQLWETTMDPSVRRLLKVQIEDAI
                     AVDQIFMTLMGDDVEPRRAFIELNALQAGNIDV"
     gene            969..1535
                     /gene="dcd"
                     /locus_tag="VFA14_02375"
     CDS             969..1535
                     /gene="dcd"
                     /locus_tag="VFA14_02375"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HZS80263.1"
                     /translation="MTIKSDKWIRRMAQQQGMIEPFEPGQVRQANGRKIVSYGTSSYG
                     YDIRCADEFKIFTNINSTIVDPKNFDEKSFVDFKGDVCIIPPNSFALARTVEYFRIPR
                     NVLTICLGKSTYARCGIIVNVTPFEPEWEGYVTLEFSNTTPLPARIYAGEGCAQVLFL
                     ESDEVCETSYKDRDGKYQGQHGVTLPRA"
     gene            1688..>2001
                     /locus_tag="VFA14_02380"
     CDS             1688..>2001
                     /locus_tag="VFA14_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007878073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HZS80264.1"
                     /translation="MAQHSSSVAADDVPAAHYLQKLLLDAIKLGASDLHFEPFEKYCR
                     VRFRIDGQLRDIAEPPLTMKDRLSSRLKILAGLDIAEKRLPQDGRMKFTLSKTHAIDF
                     RVS"
BASE COUNT          471 a          585 c          528 g          417 t
ORIGIN      
        1 acaagatcat catcatgacc gatgccgacg tcgacggcgc ccatattcgc accctgctgc
       61 tcacgctttt ttatcgtcag atgccgcaac tggtcgaacg cggtcacatc tacatcgccc
      121 agccgccgct ctacaaggtc aaggccggca aggacgagcg ttacctcaaa gacgacgtcg
      181 aagaaatgca ttacatgatg cagatcgccc tcaacgatgc cgaactcgta cctagtgaag
      241 gcgccatccc gatcagcggc gcgccgctca ttgaactggt gcgccaatac aacgtcgcca
      301 acgccatcat catgcgcctg gcgcgcgtga tcgacgatgc ggcgctgtcg gccatcatga
      361 ccggcgtcac gctgcaactg aacagtgctg ccgaagccgc gcaatccgcc gctgcattga
      421 aagccgccat caacgtgctg gatatcgatg tcatcgccta ctccgacgaa ttgactgaca
      481 gacacgccct gcgcattcta cgttcctacc acggcaacat caaagtcact gccatcgaca
      541 ccgacttcgt cgacagcccc gactaccggg tgctcagcaa tgccgctgct gtcttcaagg
      601 gtctgattgg ccgtggcgcg ctgattcgcc gcggcgctgg agacaagatg aaagaaaacg
      661 ccattgctga tttccaccag gccatggcat ggctgcgcgc cgaagctgaa cgcagcgtca
      721 gcaaacagcg ctataaaggt ctgggtgaaa tgaatccgga acagttgtgg gaaaccacga
      781 tggacccgag tgtgcgtcgc ctgctcaaag tgcagatcga agacgcgatt gccgtggacc
      841 agattttcat gacgctgatg ggcgatgatg ttgaaccgcg ccgggcgttt atcgagttga
      901 atgcgttgca ggctgggaat atcgatgttt gacttgtact acaatcgcac tctgtttcca
      961 ccaagacaat gaccatcaaa tccgataaat ggatacgccg tatggcgcaa caacaaggca
     1021 tgatcgagcc tttcgagcct ggccaggtgc gccaggccaa cggccggaaa atcgtgtctt
     1081 acggtacgtc gtcgtatggc tacgatattc gctgtgccga tgaattcaag attttcacca
     1141 atatcaattc gaccatcgtc gatccaaaaa atttcgatga aaaatccttc gtcgatttca
     1201 agggcgatgt ctgcatcatt ccgccgaatt cgttcgcgct ggcgcgcacg gtggagtact
     1261 tccgcattcc acgcaacgta ctgacgatct gtctcggcaa atcgacctac gcgcgttgcg
     1321 gcatcatcgt caacgtcaca ccgttcgagc cggagtggga aggctatgtt acgctggagt
     1381 tctccaatac cacgccgctg ccggcccgga tctatgccgg cgaaggctgc gcgcaagtgc
     1441 tgttccttga gagcgacgag gtttgtgaaa cgtcgtataa agatcgcgac ggcaagtatc
     1501 agggccagca cggcgtgact ttgccaagag cctgagtgac ctccagaagg cgaaaagctg
     1561 aaggaaactg gcaaaatcga acgataacga tatacaaaac ggcgcctggt gcgcaatatc
     1621 gaacgcttcg atttgtccat tcaggcactc gcgttggtat tttgatcagg agtgcggact
     1681 tccggccatg gcgcaacatt ccagttctgt cgcagcggac gatgtccctg ctgcccatta
     1741 tctgcaaaag ctgctgctcg acgctatcaa gcttggcgct tccgacctgc atttcgagcc
     1801 atttgaaaaa tactgccggg tccgttttcg tatcgacggc caattgcgcg acatcgctga
     1861 accgccgctg accatgaagg acaggctgtc gtcgcgcctc aaaatcctcg ccggcctgga
     1921 tatcgccgaa aagcgcctgc cgcaggatgg acgcatgaaa ttcacactgt ccaaaaccca
     1981 cgccatcgat tttcgcgttt c
//
LOCUS       DASRDH010000099         2340 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1607081,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000099 DASRDH010000000
VERSION     DASRDH010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1607081"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(118..193)
                     /locus_tag="VFA14_02385"
     tRNA            complement(118..193)
                     /locus_tag="VFA14_02385"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(158..160),aa:Phe,seq:gaa)
     gene            483..848
                     /locus_tag="VFA14_02390"
     CDS             483..848
                     /locus_tag="VFA14_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007878411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RcnB family protein"
                     /protein_id="HZS80265.1"
                     /translation="MNKKSIIFLVLALTIGLSALPVAQAERYGRDDDRHSRQGRDDRR
                     GPPGRYGERGRGVGPNHAFYRGDRLPLEYRGRQYVVENWREHHLSAPPRGYHWVQIGV
                     DYVLVAITTGVILQLFLGN"
     gene            complement(929..>2340)
                     /gene="htpG"
                     /locus_tag="VFA14_02395"
     CDS             complement(929..>2340)
                     /gene="htpG"
                     /locus_tag="VFA14_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016833356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HZS80266.1"
                     /translation="EEVADAPQGTSVTLALKPVDAEDGMPDYTDPATIRRIVRRYSDF
                     ITWPIRMTPPGTGDDQAEPETLNRMKALWARPSNDVSPEEYTEFYRHVSHDWQPPLET
                     IRLQAEGIFEYQALLFLPAHAPLDLFMRERRRGVQLYVRRVFIMDDAEQLMPVYLRFV
                     KGVIDSADLPLNVSREILQESRDVKAIREGSTKRVLSLLEELANSDEQAQKDKYAGFW
                     TEFGQALKEGIGEDAANKERIARLLRFASTHHDADAQITSLADYIGRMKEGQDKIYYV
                     TGENHHAARNSPHLEIFRKKGVEVLLLTDRVDEWMLSFLQDFDGKELASVAKGGLDLG
                     KLENEAEKKLQEETETQFKDLVEKMKTALSGKAKDVRVTFRLTDSPACLVADEHELSG
                     NLLRMLKAAGQAAPEARPILEINPDHPLVQRLKYEAARFDDWAHILFDQALLAEGGTL
                     NDPSGFVKRLNVMLLGMTK"
BASE COUNT          439 a          680 c          678 g          543 t
ORIGIN      
        1 taagtgacat ttcgccattc aggataacga catcagcttt tgaggtgcca accctaaatt
       61 cagtcaacat acttgcagtg ttgggcaaat gaccgggaac ggttggctgt ctaattctgg
      121 tggcccgggg cggaatcgaa ccaccgacac aaggattttc agtcctctgc tctaccgact
      181 gagctaccag gccaagggac cgaactatag caagataatt ccggaatgaa aagggcgact
      241 ttgctttttt ataggaaagt ttggtcgtct cgttttacgt cctttaccac aggatactgg
      301 atggatcagt ggcagggaag caccggccgc gccgaccatc tttttcgccg acgactcacg
      361 gattgcgggc gctatcgacg cgaagacgct ggaacagaaa ttcttctcta tgctgaagta
      421 agactgctat gatgagagcg ctagcaggtt tctagttcat cttctgccat caggagtcca
      481 tcatgaacaa gaagtccatt atcttcctgg ttcttgccct gacgatcggt ttgagtgctt
      541 tgcctgtcgc gcaggctgag cgatacggcc gcgacgatga tcgtcattcg cggcagggtc
      601 gggatgaccg ccgcggccca cccggtcgct atggtgagcg tgggcggggt gtcggtccca
      661 accatgcctt ctatcgcggc gatcgcctgc cgcttgagta ccgtggccgt caatatgtcg
      721 tcgaaaactg gcgtgagcac catctgagcg cgccgccgcg cggctatcat tgggtgcaga
      781 tcggcgtcga ttatgtgctg gtcgccatta ctactggcgt gatcctgcaa ttgtttttag
      841 gtaactagtc tgtataacgg aacagcaccg ccaaaatggc aatggcgacc atcagtcgcc
      901 attgcctgca ctgctgcgaa aaaacggttt acttcgtcat gcccagcagc atgacgttga
      961 gacgcttgac gaaaccggat ggatcgttca acgtgccgcc ttcggccagc agagcctgat
     1021 cgaacaggat atgcgcccag tcgtcgaatc tggctgcttc atatttcaga cgttgcacca
     1081 gtggatgatc cgggttgatt tccaggatag gcctggcttc cggcgcagct tgtccggcag
     1141 ccttgagcat gcgtagcaga ttgcccgaca attcatgctc gtccgccacc aggcaggccg
     1201 gggagtcggt caggcggaag gtcacgcgca catccttggc cttgccgctc agcgcagtct
     1261 tcatcttttc gacgagatcc ttgaactggg tttcggtttc ttcctgcagt ttcttttcgg
     1321 cttcgttttc cagtttgccg aggtcgagac cgcctttggc caccgaggcc agttccttgc
     1381 cgtcgaaatc ctgcaggaaa gatagcatcc attcgtcgac gcgatcggtc agcagcaaga
     1441 cttccacgcc tttcttgcgg aaaatttcca gatgcgggct attccttgcc gcgtgatgat
     1501 tttcgccagt gacgtaataa atcttgtcct gtccttcctt catgcggccg atgtaatcgg
     1561 ccaatgatgt gatctgcgca tccgcatcgt gatgcgttga agcaaagcgc agcagcctgg
     1621 cgatgcgctc cttgttggcg gcgtcttcgc cgatgccttc tttcagcgcc tggccgaact
     1681 cggtccagaa gccggcgtat ttgtcttttt gcgcctgttc gtcgctgttg gcgagttctt
     1741 ccagcaggct cagcacgcgc ttggtcgaac cttcgcggat cgccttgacg tcgcgcgact
     1801 cttgcaggat ttcacgcgac acgttcaatg gcagatcggc tgaatcgatc acgcctttga
     1861 cgaaacgcag gtacaccggc atcaactgct cggcatcgtc catgatgaac acccggcgga
     1921 cgtacagctg gacgccacgg cggcgctccc gcatgaacag gtccagcggg gcgtgcgccg
     1981 gtaggaacag cagcgcctgg tactcgaata tgccctcggc ctgcagccgg atcgtctcca
     2041 gcggcggctg ccagtcgtgg ctgacgtgcc ggtagaactc ggtgtactcc tccggcgaga
     2101 cgtcgttgct cgggcgcgcc cacagcgcct tcatgcggtt cagggtctcc ggctcggcct
     2161 ggtcgtcacc agttcccggc ggagtcatcc ggatgggcca ggtgatgaag tccgagtagc
     2221 gccggacgat ccgccggatc gtcgccgggt cggtgtagtc cggcatcccg tcctcggcgt
     2281 cgaccggctt gagcgccagc gtgaccgacg tgccctgcgg cgcgtcggcg acctcctcga
//
LOCUS       DASRDH010000100         5243 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_28679,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000100 DASRDH010000000
VERSION     DASRDH010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 5243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5243
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28679"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            592..3246
                     /gene="gyrA"
                     /locus_tag="VFA14_02400"
     CDS             592..3246
                     /gene="gyrA"
                     /locus_tag="VFA14_02400"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HZS80267.1"
                     /translation="MDQFAKETILISLEEEMRKSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLFAMHEMNNVWNRPFVKCARVVGEVMGKYHPHGDASIYDTLVRMAQDFSLRYTL
                     VDGQGNFGSVDGDGAAAMRYTECRLDKIANELLADIEKETVDFDPNYDGKEKEPSVLP
                     TRIPNLLINGSSGIAVGMATNIPPHNLTEVINGALHVLANADCTIDELIELIPAPDFP
                     TGGIIYGVSGVRDGYRTGRGRVVMRAKTSFEEFGRDGRTAIIVDELPYQVNKKALLER
                     IAELVRDKKLEGISDLRDESDKSGMRVVIELKRGEVPEVVLNNLYKQTQLQDTFGMNM
                     VALVDREPRLLNLKQMLEYFLSHRREVVTRRTVFELRKARERGHVLEGLAVALANIDD
                     FIAIIRAAPTPPIAKSELMARAWDSSMVREMLARAGENAGGIAAFRPENLSVHYGIQA
                     DGLYKLSDDQVQEILQMRLQRLTGLEQDKIVNEYREVMEQIADLLDILSRPARVTAII
                     TDELIAARNDFGIDAKDGRRSTIELNATDLGTEDLITPQDLVVTLSHTGYMKSQPVSE
                     YRAQKRGGRGKQAMVTKDDDWIDQLFIANTHDYILCFSNRGRLYWLKVWEVPQGSRNS
                     RGKPIVNMFPLHDGEKITVVLPLSGENRVFPEDRYVFMSTSLGTVKKTPLSDFSNPRK
                     AGIIAVDLDEGDFLIGAALTDGQHDVMLFSDAGKAVRFDENDVRPMGRTARGVRGMSL
                     DEGQQVIALLVAENEQQSVLTATENGFGKRTPITEYTRHSRGTKGMIAIQTSERNGKV
                     VAATLVEASDEIMLITTGGVLIRTRVSEIREMGRATQGVTLIAVEDGARLSGLQRVLE
                     SDAEEYKGEEGVESANDATAGEGDATAE"
     gene            3345..4442
                     /gene="serC"
                     /locus_tag="VFA14_02405"
     CDS             3345..4442
                     /gene="serC"
                     /locus_tag="VFA14_02405"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HZS80268.1"
                     /translation="MFYNFSAGPAVLPKEVLQQAADEMLDWHGSGMSVMEMSHRGKEY
                     TSIIEAALRDLRELLAIPANYRILLLQGGAIAENAVIPMNLAGRRGSVGVADYVNTGA
                     WSTRSIKEARRYCKVNVAASSEAEKFVRIPARDTWKLSRNAAYVHLCTNETIGGVEYQ
                     FTPDVAADTDNAPIVADMSSHILSRAVDVSRYGVIYGGAQKNIGPAGVTIVIVREDLL
                     GHALPICPSAFDWKIVADNDSMYNTPPTYAIYIAGLVCQWLRRQGGVAEMERRNIAKA
                     NLLYDCLDSSGFYTTRIAKECRSRMNVPFFLHDETLNETFLGGAKEYGLLQLKGHKSV
                     GGMRASIYNAMPIEGVQALVDYLRDFERTHG"
     gene            4542..>5243
                     /gene="pheA"
                     /locus_tag="VFA14_02410"
     CDS             4542..>5243
                     /gene="pheA"
                     /locus_tag="VFA14_02410"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006462114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HZS80269.1"
                     /translation="MSDDQLLPLRRKIDAIDARILDLLNQRARVAQEVGRIKAETSAP
                     VFRPEREAQVLRKVAGHNSGPLLFSDIQTIFREVMSACRALEKRVVVAYLGPEGTFSE
                     QAVYQQFGHAVTGLPCVSIDEVFRDAEAGTADFGVVPIENSAEGVINRTLDLLLQTTL
                     RIIGEVSIPVHHSLMTRTGSMQGVTRICAHSQALAQCNTWLRQNYPVIARQAVASNAE
                     AARMAGEDAGVAAIAG"
BASE COUNT         1129 a         1372 c         1563 g         1179 t
ORIGIN      
        1 tgtgcggtca cagacagggc gatggctgcg gacgtaacag cgaagacgag ttttgctaat
       61 ttgttcatgt ttctcctctc gggtgaaata tccatatccg cagataaact gcgctacaaa
      121 tacaaaatga cgacatcaaa aaaacggata gtatcatgcg tttcaggtca ccacacgtcc
      181 ctttgcaggg gacatgcaat gagcttagcc gccattctgc cacaggctgg tgcaatcggt
      241 gaacgttagc aaatttgcct ggttaacgtt ttttatactc agttgttttt tggcaacatg
      301 gtgtttaccc tctcgagcat gctcaggcgt taccggaaat gcaggttttt gcactgtatc
      361 tatcgatgtc gatgatttga attggagcgg ctggtcgtgg ggtcatcgat gatcatttcc
      421 tgtcgttgga taatgcctgt gtagcggggt tggtagtcat cgtgaccggt gacggaagcg
      481 gtcggcgcat gccctggcat gctagaatcg ctggcgttgg ccgttatcgc tgtctacata
      541 ttcctgtaga gcttgattta acgaccgagc tcaatacttc ccgacctgcc aatggatcaa
      601 ttcgccaaag aaactatcct catttccctt gaagaagaga tgcgcaagag ttatctcgat
      661 tacgccatga gcgtgatcgt ggggcgtgct ttgccggacg tgcgtgatgg cttgaaaccg
      721 gtgcatcgcc gtgtgctgtt tgcgatgcat gaaatgaaca acgtctggaa tcgcccgttt
      781 gtgaaatgcg cgcgcgtggt cggtgaggtc atgggtaagt accatccaca cggcgatgcg
      841 tcgatttacg atacgctggt gcgcatggcg caagacttct cgctgcgtta tacattggtg
      901 gatggccagg gcaattttgg ttcggtcgat ggcgatggcg ctgcggcgat gcgctacacc
      961 gagtgtcgtc ttgacaagat cgctaatgaa ctactggccg acattgaaaa agaaactgtt
     1021 gatttcgatc ccaattatga tggcaaggaa aaagaaccgt cggtgctgcc gacgcgtatt
     1081 cccaacctgt tgatcaacgg atcatccggt atcgcggtgg ggatggcgac caatatcccg
     1141 ccgcataacc tcaccgaggt catcaacggc gcgctgcatg tgctagcgaa tgccgattgc
     1201 acgattgacg agctgattga gctgattcca gcaccggatt ttccgaccgg cggcatcatt
     1261 tatggcgtgt ctggtgtgcg tgacggttat cgcaccggcc gcggtcgtgt ggtcatgcgt
     1321 gccaaaacga gcttcgagga gttcggccgc gacggacgta ctgcgatcat cgtcgatgaa
     1381 ctgccctatc aggtcaacaa gaaagccttg ctggaacgta tcgccgaact ggtgcgcgat
     1441 aaaaagctgg aaggcatttc cgatctgcgc gacgagtccg ataaatcggg catgcgcgtg
     1501 gtgatcgagt tgaagcgtgg cgaagtgccg gaagtggtac tcaataatct gtacaagcag
     1561 acgcagctgc aggacacatt cggcatgaat atggtggcgt tggtcgatcg cgaacctagg
     1621 ctgctgaatc tgaagcaaat gctggaatac ttcctgtcac atcgccgcga ggtggtcacg
     1681 cgccgcacgg ttttcgagct gcgcaaggca agggagcgcg gtcatgtgct ggaaggtctg
     1741 gctgttgcac tggccaacat cgatgatttc atcgctatca ttcgcgctgc tccaacgccg
     1801 ccgatcgcga aatctgaact gatggcacgc gcctgggatt cttccatggt acgtgagatg
     1861 ttggcgcgtg ccggcgagaa cgctggcgga atcgccgcat tccgtccgga aaatctgtct
     1921 gtacactacg gcattcaggc cgatggtctg tacaaattat ctgacgatca ggtgcaggaa
     1981 atcctgcaaa tgcgcctgca gcgtctgact ggtctggagc aggacaagat cgtcaacgag
     2041 taccgggaag tgatggagca gatcgccgac ctgctggata tcctgtccag gccggcacgc
     2101 gtgacggcga tcatcaccga tgaactgatc gccgccagga acgactttgg catcgatgcc
     2161 aaagacgggc gccgttcgac catcgagttg aacgccaccg acctcggtac cgaagacctg
     2221 atcacgccgc aggatctggt cgtgaccttg tcgcacaccg gctacatgaa gtcgcagccg
     2281 gtctccgaat accgcgcgca aaaacgcggc gggcggggca agcaggccat ggtgaccaag
     2341 gacgatgact ggatcgatca gctattcatc gccaatacgc acgattacat tctgtgtttt
     2401 tccaatcgcg gacgactgta ttggctgaaa gtgtgggaag tgccgcaagg ttcacgcaac
     2461 tcgcgtggca agccgatcgt caatatgttc ccattacatg atggtgagaa aatcaccgtg
     2521 gtgctgccat tgtccggtga aaaccgcgtc ttccctgaag accgttacgt tttcatgtcg
     2581 accagtctcg gcacggtcaa aaagacaccg ttgtccgact tcagtaatcc gcgcaaggcc
     2641 ggcatcattg cggtcgatct cgatgagggc gatttcctga tcggtgctgc gttgaccgat
     2701 ggtcagcacg acgtcatgct gttctccgat gccggcaagg cagtgcgttt cgacgaaaat
     2761 gacgtgcgtc ccatggggcg caccgcgcgc ggcgtgcgcg gcatgagcct cgatgaaggt
     2821 cagcaggtca tcgcattgct ggtggccgag aatgagcagc aatccgtact gacggcgact
     2881 gaaaacggtt ttggcaagcg cacgccgatc actgaataca ctcgccatag tcgtggcacc
     2941 aaaggtatga tcgcgatcca gaccagtgaa cgcaacggca aggtggtcgc tgccacgctg
     3001 gtggaagcca gtgacgagat catgctgatc actactggtg gcgtgctgat ccgtacccgc
     3061 gtgtcggaaa tccgcgaaat gggccgtgct acccaggggg tgacgctgat tgccgtcgaa
     3121 gacggtgcca gactgagcgg cttgcaacgc gtgcttgagt ccgacgccga agaatacaag
     3181 ggtgaggagg gtgttgagag cgccaatgat gctacggctg gcgagggcga cgcaacggcg
     3241 gaataaatcc tcatgatctg catcgatgtc gttccggcgg gcgctggagc ctgactttcg
     3301 tcaggatgat tttgtgctgc agtttttagt gttcaccctc aatcatgttt tacaattttt
     3361 ccgccggtcc cgctgtatta ccgaaagaag ttttgcagca ggctgccgat gaaatgctgg
     3421 actggcacgg cagcggcatg tccgtgatgg aaatgagtca tcgcggcaag gaatacacgt
     3481 ctatcatcga agcggctttg cgtgatctgc gtgagctgct tgcgattccg gccaactaca
     3541 ggatcctgct cctgcaaggc ggcgcgatcg ccgagaatgc cgtgattccg atgaatcttg
     3601 ctggtcggcg cggcagtgtc ggggtggcgg actatgtcaa cactggcgct tggtcgacca
     3661 ggtcgatcaa ggaagcgcgc cgctattgta aagtcaacgt cgcagcttcg tccgaagcgg
     3721 aaaaatttgt tcgcattccc gcacgtgaca cctggaagtt gtcgcgtaac gctgcttacg
     3781 ttcatctctg caccaatgag accattggtg gcgtcgaata ccagtttaca cccgacgtcg
     3841 ctgccgatac cgacaacgca ccgatcgtgg cggacatgtc ttcgcacatt ttgtcgcgcg
     3901 cggtggatgt ctccaggtat ggcgtgatct acggcggtgc gcaaaagaac attggtccgg
     3961 ccggcgttac catcgtcatc gtgcgcgaag acttgctcgg ccacgccttg ccgatctgcc
     4021 cttcggcctt cgactggaaa atcgtcgccg acaacgattc catgtacaac acgccgccga
     4081 cctatgcgat ctacattgcc ggcttggtct gtcagtggct caggcgtcag gggggcgtgg
     4141 ccgaaatgga gcgccgcaat atcgccaagg cgaatttgtt gtacgactgt ctggacagca
     4201 gcgggtttta tacgacacgc attgccaaag aatgccgttc acgtatgaac gtgccgtttt
     4261 tcctgcatga cgaaacattg aacgagacat ttctgggcgg cgccaaagag tacggtttac
     4321 tgcagttgaa aggccacaag tcggtcggtg gcatgcgcgc atcgatctac aatgccatgc
     4381 cgatagaagg tgtacaagcc ctggtggatt atctccgcga tttcgagcgg acacacggct
     4441 gagagaacgt caggccatcg ttggcggctt tgcgtctgcc tgtcatggtg gcgcgctgtc
     4501 tgcgaaccgg gcgggaacag gactggatca gaaagactac catgagtgat gatcaactct
     4561 tgccattgcg tcggaagatc gacgccatcg atgcgcgcat tctcgatttg ctcaaccagc
     4621 gcgcgcgcgt tgcgcaggag gtcggtcgca tcaaggccga aaccagcgcg ccggtgtttc
     4681 gtcccgagcg tgaagcgcag gtgttgcgca aggttgcagg acataattcg gggccgttgc
     4741 tgttctcgga tattcagacc attttccgcg aagtcatgtc ggcttgccgt gcgctcgaaa
     4801 agcgtgtcgt ggtggcctac ctcggtcccg aaggcacctt cagcgagcaa gccgtgtacc
     4861 agcagttcgg tcatgctgtc acaggtttgc cgtgcgtgtc gatcgatgaa gtgttccgtg
     4921 atgctgaagc tggcaccgct gatttcggcg tggtgccgat cgaaaattct gccgaaggcg
     4981 tgatcaatcg cacgcttgat ttactgctgc aaaccacgct gcgcattatt ggtgaagtgt
     5041 ccattccggt tcatcatagc ctgatgacca ggactggcag catgcaaggt gtcacgcgca
     5101 tctgcgcgca ttcgcaggca ctggcgcagt gcaatacctg gctccgtcag aactatccgg
     5161 tcatcgcacg tcaggcagtg gcgtcaaatg ccgaagcggc gcgcatggcc ggcgaagatg
     5221 ccggtgttgc cgccattgct ggc
//
LOCUS       DASRDH010000101         4027 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1633027,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000101 DASRDH010000000
VERSION     DASRDH010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 4027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4027
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1633027"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="VFA14_02415"
     CDS             <1..266
                     /locus_tag="VFA14_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006156448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HZS80270.1"
                     /translation="AWTIAFLTGVPGGEVMHHLQGDYVSLYVPTTPNPTSGFFLMVPR
                     ADTIELDMGVDEALRYIVSMGVVVPEIPDGKKIGTVADAATHH"
     gene            322..2127
                     /gene="aspS"
                     /locus_tag="VFA14_02420"
     CDS             322..2127
                     /gene="aspS"
                     /locus_tag="VFA14_02420"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HZS80271.1"
                     /translation="MSMRTQYCGLTTEALLGQTVSLCGWVHRRRDHGGVIFIDLRDRE
                     GLVQVVCDPDRADMFRNAEAVRNEFCLRITGVVRSRPEGTINTNLASGRIEVLAHALE
                     VLNPSVTPPFQLDDDNLSETTRLTHRVLDLRRPHMQNNLRLRYKVAMEVRKFLDAQGF
                     IDIETPMLTKSTPEGARDYLVPSRVNAGEFFALPQSPQLFKQLLMIANFDRYYQITKC
                     FRDEDLRADRQPEFTQIDCETSFLGEQEIRDLFEGMIRLVFRSALGINLPNPFPTMDF
                     ATAMSVYGSDKPDLRVKLKLTELTDVMKDVAFKVFSGAANSEGGRVVGLRVPGGAEMP
                     RSEIDAYTQFVAIYGAKGLAYIKVNEKARGRDGLQSPIVKNIHDAALVAIIEKTGAQN
                     GDLIFFGADRAKVVNDAIGALRVKIGHSDFGKKNGLFDDAWRPLWVVDFPMFEYDEDN
                     QRWIALHHPFTAPKDGHEDFISTNPGAVIAKAYDMVLNGWELGGGSVRIHRADVQSKV
                     FRALKIDAEEARLKFGFLLEALQYGAPPHGGIAFGLDRLVTMMTGAESIRDVIAFPKT
                     QRAQCLLTQAPSTVDEKQLRELHIRLRNAGPADKA"
     gene            2188..2688
                     /gene="nudB"
                     /locus_tag="VFA14_02425"
     CDS             2188..2688
                     /gene="nudB"
                     /locus_tag="VFA14_02425"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013232284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HZS80272.1"
                     /translation="MNCPLKIPESVLVVIHSEKLDVLLIERADKAAYWQSVTGSRDTW
                     DEPLHETARREVGEETGIIISDTGSAAPAATNRVPCNHLRDWHLSSVYEIYPFWRHRY
                     ASGVTHNTEHVFGLLVPRDIPVTLAPREHMRYQWLPYREAARQCFSPSNAEAILQLPH
                     YAASVE"
     gene            2732..3496
                     /locus_tag="VFA14_02430"
     CDS             2732..3496
                     /locus_tag="VFA14_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HZS80273.1"
                     /translation="MKLRIVTYNIHKGVTFFSGRSCIHALRQALAPLDADVLFLQEVQ
                     GRCDRSAMRQSQHEYLAGESYHAAYGMNAVYTHGHHGNALLSRFPIASVRNQDVSDHA
                     CESRGILHCVIDVAGAEVHCYVIHLGLFAGSRRRQAAALIEMVRVSSPDTPLIIAGDF
                     NDWGNQLGECLRRYLGVTEVFEQQAAALGGSLREPGGVRPIVQPARTFPARMPCLRLD
                     RIYLRGFEVESAQVLRGSLWSTLSDHLPIISTLHLK"
     gene            3515..>4027
                     /locus_tag="VFA14_02435"
     CDS             3515..>4027
                     /locus_tag="VFA14_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006464127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HZS80274.1"
                     /translation="MHAITYSGGNDVALLRSGVEFFPALIAAIDEARAEIYLETYIFR
                     IDGIGILVRDALCRAAARGVSVSVISDWLGTGRVQSRQLKKTLTADGIAYRSFNPWFR
                     RGVARDHRKLCVVDRKYAFVGGLNIVDDFINDVSHIVLPAPRWDFAVRIEGPLVQVIH
                     QEMQNQWIRIG"
BASE COUNT          819 a         1117 c         1200 g          891 t
ORIGIN      
        1 gtgcgtggac aatcgctttc ctgaccgggg ttcctggcgg cgaagtgatg catcatctgc
       61 aaggcgacta cgtcagcctg tacgtgccga ctacaccgaa tccaacctcg ggctttttcc
      121 tgatggtgcc gcgcgccgat accatagaac tggatatggg cgtcgatgaa gcgctcaggt
      181 acatcgtctc gatgggcgtg gtagtgccgg aaataccgga cggcaagaaa atcggaaccg
      241 ttgccgatgc ggcgacgcat cattgaaggc cagcctcgga aaataccact gaatcagaaa
      301 aactgtaacc ggaacccgaa tatgtccatg cgaacccaat actgcggcct taccactgaa
      361 gcattgctcg gccagaccgt cagcctgtgc ggctgggtgc atcgtcgccg cgatcacggt
      421 ggcgtgattt tcatcgatct gcgtgaccgt gaaggtctgg tacaggtcgt ctgcgatcca
      481 gaccgcgccg atatgttcag gaatgcggaa gccgtgcgca acgaattctg cctgcgcatc
      541 accggcgtcg tgcgcagtcg tcctgaaggc accatcaaca ccaatctggc ttcgggcagg
      601 atcgaagtgc tggcgcatgc gctggaagta ttgaatccat cggtcacgcc gccgttccag
      661 ctggatgatg acaatctgtc ggaaaccacc cgtctgacgc accgtgtact cgatctgcgc
      721 cgtccgcaca tgcaaaacaa tctgcgtctg cgttataagg tagcgatgga agtgcgcaaa
      781 ttccttgacg cgcaaggttt catcgacatc gaaacgccga tgctgaccaa gtcgactccg
      841 gaaggcgcgc gtgactacct ggtgccatcg cgtgtgaacg ccggcgaatt cttcgcgttg
      901 ccgcagtcgc cgcagctatt caagcaattg ctgatgattg ccaatttcga ccgctattac
      961 cagattacca aatgtttccg cgacgaggat ctgcgcgccg accgtcagcc tgaattcacg
     1021 cagattgact gtgaaacttc ctttctcggc gagcaggaaa ttcgcgattt gtttgaaggc
     1081 atgattcgtc tggtattcag gagcgcgctc ggcatcaatc tgccgaaccc gtttccgacc
     1141 atggacttcg ctacggcgat gagcgtatac ggttcggaca aaccggacct gcgtgtgaag
     1201 ctgaaattga ccgaactgac cgacgtcatg aaggatgtcg cgttcaaggt gttctcaggc
     1261 gcagccaaca gcgaaggtgg ccgcgtggtc ggtctgcgcg tgccgggcgg tgcggaaatg
     1321 ccgcgttcgg aaatcgacgc ctatacccag tttgtcgcca tttacggcgc caagggtctg
     1381 gcctatatca aggtcaacga aaaggccagg ggccgcgacg gcctgcaatc gccgatcgtc
     1441 aagaatattc atgacgccgc gctggttgcg atcatcgaga aaaccggagc gcaaaatggc
     1501 gacctgattt tcttcggtgc cgaccgcgcc aaggtcgtca acgatgccat cggcgcgctg
     1561 cgcgtgaaga tcggccatag cgacttcggc aaaaaaaacg gactgttcga cgacgcctgg
     1621 cgtccgctgt gggtggttga tttcccgatg tttgaatatg acgaagacaa ccagcgctgg
     1681 attgccctgc atcatccgtt caccgcgccc aaggatggtc acgaagactt catttcgacc
     1741 aatccgggtg cagtgatcgc caaagcctat gacatggtct tgaacggttg ggaactgggt
     1801 ggcggttcgg tgcgtatcca ccgcgccgat gtgcagagca aggtgtttcg cgcgctgaag
     1861 atcgacgcag aagaagcgcg cctgaagttc ggctttctgc tggaagcgct gcaatacggc
     1921 gcacctccgc atggaggcat tgccttcggt ctggatcgtc tggtcaccat gatgactggc
     1981 gccgagtcga tccgcgatgt gatcgctttc ccgaagaccc agcgcgcgca atgtctgttg
     2041 acgcaggcgc catcgaccgt cgatgaaaag cagctgcgcg aactgcatat tcgtctgcgc
     2101 aatgcagggc cggcggacaa ggcctgaata tggccttgat ggatactgtc tgcgcctgat
     2161 ccatgccgac gaatgcactc cggaaacatg aattgccctt taaaaattcc ggagtccgtt
     2221 ctggtcgtca tccattccga gaaactggat gtgctgctga tcgaacgcgc cgacaaagcc
     2281 gcttactggc agtccgtgac cggctccaga gatacctggg acgaaccgtt gcacgagacg
     2341 gcgcggcgcg aggtcggtga agaaaccggt atcatcatca gcgatacagg cagtgctgcc
     2401 cctgcagcca ccaaccgcgt tccatgcaat catttgcgcg actggcattt atctagcgtg
     2461 tacgagattt atccattctg gcgccatcgc tatgcgtccg gcgtgacgca caatactgag
     2521 catgtattcg gtctgctggt gccacgggat ataccagtga cgctggcgcc gcgcgagcac
     2581 atgcgttacc agtggctgcc gtatcgcgaa gcagcccgcc agtgcttttc gccatcgaat
     2641 gcggaagcga ttttgcagtt gccgcattac gctgcgtcgg tagaatgaga gccgctcatt
     2701 tttgcctgaa gcaagggatt ttgccgatcg tatgaaacta cgcattgtca cttacaacat
     2761 tcacaaaggc gtgacttttt tcagtggtcg atcgtgtatc catgcgctca ggcaggcgct
     2821 ggcgccgctc gacgccgatg tgctgttttt acaggaagtg cagggacggt gcgatcgcag
     2881 cgctatgcgt cagtcgcagc atgaatacct ggctggggag tcgtatcacg ccgcttacgg
     2941 catgaacgcc gtctatactc atggccatca tggtaatgcc ttgctgtcgc gttttcccat
     3001 cgcctcggtg cgtaatcaag atgtctctga ccatgcttgt gaatcgcgcg gcattctgca
     3061 ttgcgtgatc gatgtggcgg gcgccgaggt gcattgttac gtgattcatc tcggtttgtt
     3121 cgccggcagc cgcaggcgcc aggcagcggc gctgatcgag atggtacgtg tttcgtcgcc
     3181 ggatacgccg ctcattatcg ccggcgattt taacgattgg ggtaaccagc ttggcgagtg
     3241 cctgcggagg tatctgggtg tgaccgaggt ctttgaacag caggctgcgg cgctcggcgg
     3301 gtctctgcgc gagccaggcg gcgtgcgtcc gattgtgcag ccagcccgca ctttccccgc
     3361 gcgcatgccg tgcttgcggc tggaccgcat ttacctgcgc ggtttcgaag tggaatcggc
     3421 gcaagtgttg cgcggcagct tgtggtcgac gctgtcggat catctgccca ttatcagcac
     3481 gctgcacctg aaatgatcct gatgtttttg gcccatgcac gcgatcactt atagcggtgg
     3541 caacgacgtc gctttgctgc gcagcggtgt ggaatttttt cctgcattga ttgcggcaat
     3601 tgacgaggcg cgcgcggaaa tttacctgga gacttatatc ttcagaatag atggcattgg
     3661 cattctggta cgcgatgcct tgtgccgcgc tgctgcgcgc ggcgtcagcg tcagtgtcat
     3721 cagtgactgg ctcggtactg gtcgagtcca gagcaggcag ctcaagaaga cactgaccgc
     3781 agacgggatc gcgtatcgtt ccttcaatcc ctggtttcgg cgcggcgtgg cgcgtgacca
     3841 tcgcaagctg tgcgtggtgg atcgcaaata cgcatttgtc ggcggcctga atatcgtcga
     3901 cgatttcatc aacgatgtca gccatatcgt gttgccggcg ccgcgctggg atttcgccgt
     3961 gcgtatcgaa gggccgctgg tgcaggtgat tcatcaggaa atgcagaacc agtggatacg
     4021 catcggc
//
LOCUS       DASRDH010000102         4007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_385780,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000102 DASRDH010000000
VERSION     DASRDH010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 4007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4007
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385780"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..125)
                     /locus_tag="VFA14_02440"
     CDS             complement(<1..125)
                     /locus_tag="VFA14_02440"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HZS80275.1"
                     /translation="MPFRRRQSGLSLIELMIAMALGMGVVLASLALLQAARASYVA"
     gene            complement(126..671)
                     /gene="pilV"
                     /locus_tag="VFA14_02445"
     CDS             complement(126..671)
                     /gene="pilV"
                     /locus_tag="VFA14_02445"
                     /inference="COORDINATES: protein motif:HMM:TIGR02523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification protein PilV"
                     /protein_id="HZS80276.1"
                     /translation="MRLHVNKRCRNTSERGFTLIEVLIAIAILTLGAGGVAAMQLHAL
                     RTARHSSYQTSATQLAVELADIMRANPEAAHSSVNPYLFSYQADAQVTATSSLSPCVL
                     HTCDAAAMAHADVAGWQQRLQQSIPLASAVVCRDSHPAMTHGLRWDCDHAGHAAIIIK
                     IGWPDSPALASGPAIAIAVEI"
     gene            complement(631..1146)
                     /locus_tag="VFA14_02450"
     CDS             complement(631..1146)
                     /locus_tag="VFA14_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013236098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HZS80277.1"
                     /translation="MRDFEKKVCNRADLQARVAALPQPVVLTNGVFDILHRGHVTYLA
                     QARAEGASLVVAVNTDASVRRLGKGDDRPINTCEDRMAVLAALESVSLVLPFDEDTAL
                     EVVQEARPHVYVKGGDYDMAAIPEGRAVAAYDGKTVAIAFEHDRSTTKLLAKVRKNHA
                     TPCKQKMQEHQ"
     gene            complement(1227..2084)
                     /locus_tag="VFA14_02455"
     CDS             complement(1227..2084)
                     /locus_tag="VFA14_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008115381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HZS80278.1"
                     /translation="MLYPELFKSLEQVRWNMDKDIPWDKFDASMLSDEQAQTIRMNAI
                     TEWAALPATEMFLRDNRGDSDFSAFMSVWFFEEQKHSLVLMEYLRRFKPEFVPTDAEL
                     HNVRFEFDPAPPLETLMMHFCGEIRLNHWYRCASAWHTEPVIKRIYKIISQDEARHGG
                     AYLRYMKKALVEAGDTARAAFAKIGVLMASARRTEKPLHPTNLHVNQGLFPNDTIQSR
                     LPDPEWLEHWLGMQIKFDGEWEKKVVDRILHNMSLLFERTFDTVQELNRYRKEVVARM
                     ATLTQAASV"
     gene            complement(2180..2779)
                     /locus_tag="VFA14_02460"
     CDS             complement(2180..2779)
                     /locus_tag="VFA14_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007879544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhaM family polyhydroxyalkanoate granule
                     multifunctional regulatory protein"
                     /protein_id="HZS80279.1"
                     /translation="MLNHSNMPSACTMEFMRRMWGGMGASAMGTPSLSVEEINKKITD
                     LETVASWLELNMNILRATIQTLEVQSATLFTLQAMSATLTPHEGDGVADAASAPPSDE
                     NAAEAMQLAGQQVKPMADLQTVMASSNVWWRLLQEQFQQAVGNVLTDQSPMDEDVKAA
                     AAPKKTTKPRTEAKSRTRAKSGVIQSRQSRSASSKLASP"
     gene            2897..3958
                     /locus_tag="VFA14_02465"
     CDS             2897..3958
                     /locus_tag="VFA14_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HZS80280.1"
                     /translation="MTETKASPSSERWCDVMLFLFVALLHLFAIHRGKEFFNTTNTDV
                     PATSISVVSVLLPKQAVSLPVETKPEAPRTGPRLTPVIAPAAAPAVTEVPAALPSVSV
                     NVDGSDTAPQAAPVPTLTEEKKQPDSAPGTQYQFNPPPSSNLAYDVHAFFDKLNWYGS
                     STLTWETDGSRYTVTGAVHVHMFARITFLSFTSSGEINDSGVAPELYTEKKRNRAATN
                     THFNRERNLVSFSSSTTTYPRIGGEQDRASIIWQLVAIGRGDASQFAPGAVIDMFVAG
                     VRDGEVWRIQIVGQEEIHLSDGAHQAWHIVRQPRPGSYDQRLDIWLDPGRQWYPVRLR
                     FTETSGDYLDMLLSNPDTR"
BASE COUNT          856 a         1230 c         1045 g          876 t
ORIGIN      
        1 gcgacatagc tcgctctggc agcttgcagc aaggccagcg atgccagcac aacacccatg
       61 cctagcgcca tggcaatcat cagctcgatc aggctcaggc cgctttgccg gcgtcggaac
      121 ggcattcaga tttccaccgc aatggcgatg gcaggcccgg atgcgagcgc cggactgtcc
      181 ggccaaccga ttttgatgat gatggcggca tggccggcat gatcgcaatc ccagcgcagg
      241 ccatgtgtca ttgccgggtg actgtcgcgg cacaccacag cgctggccag cggtatcgac
      301 tgctgcagac gctgctgcca gccggctaca tcagcgtgcg ccatggcagc agcgtcacac
      361 gtgtgcagga cgcatggcga tagagatgac gttgccgtga cctgtgcatc agcctgatac
      421 gaaaacagat agggattaac actgctgtgc gcggcctccg gattggcgcg catgatgtcg
      481 gccagttcga cggccagttg cgtggcgctg gtctgatagc tgctgtgcct ggccgtgcgc
      541 aaggcgtgta actgcatggc ggcaacgccg ccggcgccga gcgtcaggat cgcgatggcg
      601 atcaatactt cgatcagcgt gaagccgcgt tcactggtgt tcctgcatct tttgtttaca
      661 tggagtcgca tgatttttgc gcactttcgc cagcagcttg gttgtcgaac gatcatgctc
      721 aaaggcaatt gccaccgtct tgccgtcata tgccgccacg gcccggcctt ccggaatcgc
      781 cgccatgtca tagtcgccac ccttgacgta aacgtgagga cgcgcttcct gcacaacttc
      841 cagcgccgta tcttcatcga aaggcaacac cagcgatacc gactccagcg ctgccagaac
      901 ggccatgcga tcctcacagg tattgatcgg cctatcatcg cctttgccca gacgcctgac
      961 ggaagcatcg gtattgaccg ccactaccag cgatgcgcct tcggcgcggg cctgcgccag
     1021 ataggtcaca tggccgcggt gcagtatatc gaatacgcca ttggtcagca ccaccggttg
     1081 cggcaacgcg gcaacgcgcg cctgaagatc ggcgcgatta cagacttttt tctcgaaatc
     1141 acgcattcgt cattatccac aagtattttg cttccataag aaaagccgca cctcgggtgc
     1201 ggctttctac ctcaaaacca accagatcaa actgacgcag cttgcgtcaa tgtcgccatg
     1261 cgcgcaacga cttcctttcg gtagcggtta agctcctgca cggtatcaaa agtacgctcg
     1321 aacagcagcg acatattgtg caaaatacgg tcgacgactt tcttttccca ctcgccgtca
     1381 aacttgatct gcatgcccaa ccagtgttcc agccactccg gatccggcaa acggctttgt
     1441 atggtgtcgt tcggaaacaa tccctgattg acatgcagat tcgtcggatg cagcggtttt
     1501 tcggtgcgtc tggccgatgc catcagcaca ccgatcttgg caaacgcggc acgcgcggtg
     1561 tcgccggctt cgaccagcgc ttttttcatg tagcgcagat aagcgccacc atggcgagcc
     1621 tcatcctggc tgatgatctt gtagatccgc ttgatgaccg gctcagtgtg ccaggcggaa
     1681 gcgcagcggt accaatggtt caggcgaatt tcaccgcaaa aatgcatcat cagcgtttcc
     1741 agcggcggcg ccggatcgaa ctcgaaacgc acgttatgca gttctgcgtc ggtcggcacg
     1801 aattcaggct tgaaacggcg cagatactcc atcagcacca gagaatgttt ttgctcttcg
     1861 aaaaaccaca ccgacatgaa agcggaaaag tcactgtcgc cgcgattatc acgcaaaaac
     1921 atttccgtcg cgggcaacgc cgcccactcg gtgatcgcat tcatgcgaat ggtctgggcc
     1981 tgctcgtcgg aaagcatcga tgcgtcgaat ttatcccagg gaatatcctt atccatattc
     2041 cagcggactt gttcgagtga tttaaacagt tcgggataca gcatgctgac cttgctcagg
     2101 gtggattaat ttatcgattt caccaggagt ttcccctcac ccactcgcac atatctgcgc
     2161 agttgtcata ttgcaccgat caaggggatg ccagcttgct ggaagctgac ctggattggc
     2221 gggattgtat aacacccgac ttggcgcgtg ttcgcgattt agcctccgtc ctgggttttg
     2281 ttgttttttt cggcgcagca gccgctttga catcctcatc catcggcgat tgatcagtca
     2341 atacattgcc gacagcctgc tgaaattgct cctgcagcag cctccaccaa acattcgaac
     2401 tggccattac agtctgcaaa tccgccatcg gcttgacctg ctgaccagca agctgcattg
     2461 cttctgcggc gttttcatct gatggcggcg cgctggcggc atccgccacc ccatcgcctt
     2521 cgtgcggcgt cagcgtagcg ctcattgcct gcaacgtgaa cagcgtggcg ctttgcactt
     2581 ccagcgtctg tatagtggcg cgtaatatgt tcatgttgag ctccagccat gacgctacgg
     2641 tttccagatc agtgattttc ttgttgattt cttcgaccga caacgacgga gtacccatcg
     2701 ccgacgctcc cataccgccc cacatcctgc gcatgaattc catggtacag gcgctgggca
     2761 tgttgctatg attcagcatt tcagtctccc atcgggtcat tcaactttca tacgcacacc
     2821 accgcaatat tccgatcatg cacttcctct gcaagctacg caacgatgca ggcaagactc
     2881 gcgtcacact gacgtcatga cagaaaccaa ggcatctcct tcttccgagc gctggtgcga
     2941 cgtcatgctg ttcctgtttg tcgcgctgct gcatctgttc gcgattcaca ggggtaaaga
     3001 attcttcaac accaccaaca ccgatgtacc agccaccagt atcagcgttg tttcagtgct
     3061 gctgccgaaa caagcggtat cgctaccggt ggagaccaag cctgaagcgc cccgaaccgg
     3121 accccgcctg acaccggtca tcgcgccggc cgccgcgcct gctgtcaccg aggtgccagc
     3181 ggcgttgcca tccgtatccg tcaatgtgga tggtagcgat accgcgccgc aagcggcacc
     3241 cgtccctacc ctgacagaag aaaagaagca gcccgacagc gcaccaggca cgcagtacca
     3301 gtttaatcca ccgccatcgt ccaatctggc ctacgacgtc cacgccttct tcgacaagct
     3361 gaactggtac ggcagcagca cgcttacctg ggaaaccgac ggcagccgct ataccgtcac
     3421 tggcgcagtg catgtccata tgttcgccag gatcaccttc ctgagcttca ccagcagtgg
     3481 cgaaatcaac gattctggcg tcgcgccgga gctgtacacg gaaaagaaac gcaaccgcgc
     3541 cgccaccaac acccatttca atcgcgagcg caatctggtc agtttttcgt cttccaccac
     3601 tacttatccg cgtatcggcg gcgagcaaga ccgcgccagc atcatctggc aactggtcgc
     3661 aatcggccgc ggcgacgcca gtcaattcgc accaggcgcg gtcattgaca tgttcgtcgc
     3721 tggcgtgcgt gatggcgaag tttggcgcat acagattgtc ggtcaggaag aaatccattt
     3781 gtccgatggt gcgcatcaag cctggcacat agttcgccag ccgcgcccag gctcctacga
     3841 ccagcgtctc gatatttggc tggatcccgg tcggcaatgg tatccggtgc ggctgcgctt
     3901 taccgaaacc agcggagact atctggacat gttgctgtcc aatcccgaca ctcgctagcc
     3961 cattgaacca attgaattac actggaactc atttcacaaa tagtgac
//
LOCUS       DASRDH010000103         3079 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1680974,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000103 DASRDH010000000
VERSION     DASRDH010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 3079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3079
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1680974"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..944)
                     /gene="argF"
                     /locus_tag="VFA14_02470"
     CDS             complement(30..944)
                     /gene="argF"
                     /locus_tag="VFA14_02470"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HZS80281.1"
                     /translation="MAIKHYLRFSDFTLDEYAYVIERSHVIKRKFKNYEPYHPLADRT
                     LIMVFEKNSTRTRLSFEAGMHQLGGAAIYLNTRDSQLGRGEPIEDAAQVMSRMCDVIM
                     LRTFGQEIIERFAAYSRVPVINGLTNEYHPCQVLADVFTYIEQRGSIEDKVVAWVGDA
                     NNMLYSWLQAAEVFGFHVNVSTPRGYDIDHTLVAPGHQRYTFFSNPADACQDADLVTT
                     DVWTSMGYEAENAMRLQAFDGWIVDQAKMARAKKDALFMHCLPAHRGEEVSAAVIDGP
                     QSVVWDEAENRLHVQKALLEYLVLGKVA"
     gene            complement(1118..1654)
                     /locus_tag="VFA14_02475"
     CDS             complement(1118..1654)
                     /locus_tag="VFA14_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013233743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HZS80282.1"
                     /translation="MPAPSLKRRLCSMAYESILLFGVLLVSAWIFSTLLQQRHALYLR
                     HALESWLFVTLCLYFVWFWNHGGQTLAMKTWRIRLVGKDGAKVSAARAFVRFLLGWLW
                     FLPGLALAWKWGARGWMLVWIPAINIGVWTALAYLDPARQFLHDRMAGTRLIDLHCPT
                     GNDRADASASRSQEPHAR"
     gene            complement(1745..2359)
                     /locus_tag="VFA14_02480"
     CDS             complement(1745..2359)
                     /locus_tag="VFA14_02480"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006712884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HZS80283.1"
                     /translation="MQIRLFPTAHQQAYCMATDKELSMFLENAERRAFRQAIYAVRTD
                     EAALDIVQDAMTKLAEKYGDKPADALPLLFQRILQNTIHDFFRQQKIRGTWVSLFSSI
                     SRDTESQEHFDLLENYEAEEGTQTAESSADRVEREQTLSLIDAEVQKLPTRQREAFLM
                     RYWQDMDVAETASAMGCSKGSVKTHCSRAAHALAQALRAKGISL"
     gene            2811..>3079
                     /locus_tag="VFA14_02485"
     CDS             2811..>3079
                     /locus_tag="VFA14_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HZS80284.1"
                     /translation="MSVENLTGAEILVRCLAEEGVEHVFGYPGGAVLYIYDAIFKQNG
                     FQHILVRHEQAAVHAADAYSRSSGKIGVCIVTSGPGVTNAVTGLAT"
BASE COUNT          672 a          816 c          925 g          666 t
ORIGIN      
        1 cgagagcacg ttctgagcgg cagacacgat catgccactt ttcccagtac gagatattca
       61 agcagggctt tttgcacatg caggcgattt tccgcttcgt cccagaccac cgattgtgga
      121 ccgtcgatga ctgcggcgga gacttcctcg ccgcgatgcg ccggcaggca gtgcatgaat
      181 aaggcgtctt ttttggcgcg ggccatcttg gcctgatcga cgatccagcc gtcgaaagct
      241 tgcaggcgca tggcgttttc agcttcgtaa cccatgctgg tccagacatc ggtcgtgacc
      301 agatcggcat cctggcaggc gtcggccgga ttgctgaaga aggtgtagcg ctgatggccg
      361 ggcgcgacca gcgtgtgatc gatgtcgtag cccctggggg tggatacatt gacgtgaaaa
      421 ccgaatactt cggcagcctg cagccacgaa tacagcatgt tgttggcatc gccgacccag
      481 gctacgacct tgtcctcgat cgagccgcgc tgttcgatgt aggtaaacac atcggccagc
      541 acctggcagg gatggtattc attggtcagg ccattgatga ccggcacgcg cgaataggcg
      601 gcgaagcgct cgatgatttc ctggccgaag gtgcgcagca tgatgacgtc gcacatgcgc
      661 gacatcacct gcgccgcgtc ttcgatcggc tcgccgcggc cgagctggct gtcgcgggta
      721 ttgaggtaga tcgccgcgcc gccgagctgg tgcatgccgg cctcgaagga caggcgggtg
      781 cgcgtggagt ttttctcgaa caccatgatc agtgtgcgat cggccagcgg atgatacggc
      841 tcatagttct tgaatttgcg tttgatcaca tgcgagcgct cgatcacgta ggcatattca
      901 tcaagcgtga agtcggaaaa ccgcaggtag tgtttgatgg ccatgaatga aagcggcggc
      961 cagtgaggcg cctgccgccg gttgtgatga ctgcctcaga ttataaggca tccaggttga
     1021 aaagatacgc aaaggcagat atcgctttgc acagtacgtg cagagcgact agaacgtagc
     1081 gtcacgatat gccaggagaa atgcgctgtg atgaccatca gcgtgcgtga ggctcttgtg
     1141 agcgggatgc cgatgcgtcg gcgcgatcat ttccggtggg gcagtgtaaa tcgatgaggc
     1201 gtgtgccggc cattctgtcg tgcagaaact gccgtgcagg gtccagatag gccagggctg
     1261 tccagacgcc gatgttgatg gcgggaatcc ataccagcat ccatcccctg gcgccccatt
     1321 tccatgccag cgccagaccc ggcaggaacc acagccagcc cagcaggaaa cggacgaagg
     1381 cgcgtgcggc gctgaccttg gcgccgtctt tgccgaccag gcgaatacgc caggttttca
     1441 tcgccagcgt ttggccacca tggttccaga accagacgaa atacaggcag agggtgacaa
     1501 acagccagct ttccagtgca tggcgcagat agagggcatg gcgttgttgc agcagcgtgg
     1561 aaaatatcca ggccgagacg agcagcacgc caaacagcaa tatggattca taggccatgc
     1621 tgcacagacg gcgtttcaga gaaggggccg gcatggtgcg ccgtgccgtt tccggtactg
     1681 aattcaatat aatctccagt atcgagtgcc ggatttaccc gacaggcaaa cggtagttcc
     1741 tgatttataa actgattcct ttagccctga gcgcttgtgc cagggcatgt gctgcgcgag
     1801 aacagtgggt cttgacgcta cccttggagc atcccattgc gctagctgtt tcagcgacat
     1861 ccatgtcttg ccagtaacgc atcagaaaag cttctcgttg acgcgtcggg agtttctgca
     1921 cttctgcatc gatcaaagag agcgtttgct cgcgctcgac cctgtcagcg ctggattcgg
     1981 cggtctgggt gccttcttcc gcttcgtaat tttccagcag gtcgaagtgt tcctggcttt
     2041 cggtatcgcg gctgatgctg gaaaacagac tgacccaggt gccgcggatt ttctgctggc
     2101 ggaaaaaatc atgaatggtg ttttgcagga tgcgctgaaa cagaagcggg agcgcatccg
     2161 ccggtttgtc gccgtatttt tcagccagct tggtcatggc gtcctgaacg atgtcaagcg
     2221 cagcttcatc tgtgcgcacg gcgtagatgg cctgcctgaa ggcgcgccgc tcagcatttt
     2281 cgagaaacat ggagagttct ttgtcagttg ccatgcagta ggcttgttgg tgcgcagtcg
     2341 gaaacagacg aatctgcatg cacttccaac atgtccagca agagcgcatg ctagcaagaa
     2401 aatatttgtt tgtggcggta tcggccagaa atatacgatg cgtaagcggt tttccaggtt
     2461 gtcgaatgaa attcacttga ccaaaatgat gcggggcatt aatttatcaa tccgcttcat
     2521 caacatgaag cgcctttgcc gtggcgacgc atcatgtagc gatgtccaag gcgcgcttca
     2581 ttgaaaagct gtttgttcca acccgcgcca caagcgcgag aaattcgtaa cggtgtgctt
     2641 gcaccaaggc tgaacgagtc gcgctgagcg gcattccaga cctcactacg gttgaggggc
     2701 aggaatgacg tgaccgcaca ggaaagggat gccggagcaa gtcgatcagc acggtttcgt
     2761 caggagagaa catccgatca atctcccatg gaccttgaaa ggaagtagtc atgagtgtag
     2821 aaaatctcac cggcgcggag atactcgtcc gttgcctggc tgaagagggc gtcgagcatg
     2881 tcttcggcta cccgggcggc gccgtgcttt atatatacga cgccatcttc aaacagaacg
     2941 gattccagca tattctggta cgccacgagc aggctgcggt acatgcggca gatgcgtatt
     3001 cgcgcagttc cggaaaaatc ggcgtatgca tcgtgacctc cggtcccggc gtcaccaatg
     3061 cggtgaccgg tctggcgac
//
LOCUS       DASRDH010000104         2328 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1175536,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000104 DASRDH010000000
VERSION     DASRDH010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1175536"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="VFA14_02490"
     CDS             complement(<1..455)
                     /locus_tag="VFA14_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HZS80285.1"
                     /translation="MRRAIGVFFCLLIMAVLVIVLSSACGSTGCLRPGGNVDAALLLQ
                     LRVPRALTAFTVGALLAMAGALMQILLRNPLAEPYVLGVSGGAATGALLALLSAIAYP
                     GLARHDVTAVGAALGAGIAVLLLFGLAGKSLRRLSLSPMAAGHRLLLTGV"
     gene            complement(463..2217)
                     /locus_tag="VFA14_02495"
     CDS             complement(463..2217)
                     /locus_tag="VFA14_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008118032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HZS80286.1"
                     /translation="MADDIRHAGIDNVNEAIRKLGGVYGRQSLTGSSDFSLDMRGFGS
                     NGNQNLVILVDGVRLSENELATALLSSVPIDTVDRIKIVRGGGSVLYGDGATGGTIRI
                     ITRQPTADTVHGTVIVEVGDDGLRSGRSIVSKGWDGFALHTAYSRQHTDNYRDNNASS
                     QKNFSGGAQWFSSLGRLGLRVDLARSDYGLAGALSMAQFLANPRQTATPLDHGAYDSD
                     RVTVFFERRFDTLEAAAELSHHEKISRSSYGGGAVNEVHSRTTQFSPRLRRIVEGGVL
                     KNELVTGLDFSQWQTGRIKGHSDAAQTAHAAYVRNELHIVENTRIAFGLRHEVVDKNS
                     VSGSYDQTQGIGAWEVQASHAVVPSTRLFSKAGRSYRLPTADENGFTALAMGRILKPQ
                     MSHDLEFGVTWGGAGTNATLTWFRHRLKDELFFDPTAGAFGVNVNLDPTRRQGVELEA
                     NARLNADFSLKVNFQHVAATFTDGRHVGKELVLVPKNTTSARLNWQSGRQTINLGVLW
                     ADTQRYGNDFNNTCNTRMPSYATIDARYAARARAWEFALTSSNLTDKNYFSQAFGCAS
                     GIYPESGRALKFTARYDF"
BASE COUNT          495 a          708 c          672 g          453 t
ORIGIN      
        1 acgcctgtca gcagtaagcg atggcctgcg gccatcggcg acaatgacag gcgtcgcaga
       61 gattttccag ctaggccgaa cagcagcagc acggcaatgc cagcacccag tgccgcgccg
      121 acggccgtga cgtcgtggcg cgccagtccg ggataagcaa tggccgacag cagggccagc
      181 aaagcgcctg tagcagcgcc gcccgatacg ccaaggacat aaggctcggc cagtggatta
      241 cgcaagagga tttgcatcag cgcgccggcc atggccagca gcgcgccaac cgtaaatgcc
      301 gtcaacgcgc gcggcacgcg caattgcaat agcagcgccg catcgacatt gcctcccgga
      361 cgcaggcagc cggtgctgcc acatgcgctg gagagtacga tgaccagcac agccatgatg
      421 agcagacaaa aaaacactcc tatcgcgcgt cgcatgcagg ccctagaagt cataacgcgc
      481 ggtaaattta agcgcacggc cactctctgg atagataccg ctggcgcaac cgaaagcttg
      541 cgagaaatag ttcttgtcag tcaggttgct gctggtcagc gcgaattccc acgcgcgcgc
      601 gcgcgcggca tagcgtgcat ctatcgtggc gtatgacggc atgcgcgtat tgcaggtatt
      661 gttgaaatca ttgccgtagc gctgcgtatc ggcccacagc acaccgaggt tgatggtctg
      721 cctgccactt tgccagttca gacgcgccga agtggtattc ttcggcacta gcaccagttc
      781 cttgccaaca tgccggccgt cagtaaatgt cgcggcgacg tgctggaaat tcaccttcag
      841 gctgaagtcc gcattcaggc gtgcattggc ttccagctcc actccctgcc gtctggtcgg
      901 atccagattg acattgacgc caaaggcgcc ggccgtagga tcgaagaaca attcatcctt
      961 gagacgatgg cggaaccacg tgagcgtggc attggtgccc gcacctcccc aggtcacgcc
     1021 gaactccagg tcatgcgaca tctgcggctt gaggatgcgc cccatcgcca gtgcggtgaa
     1081 accattttca tcagcagtcg gcaaacgata gcttcgccca gccttgctga atagacgcgt
     1141 cgatggcacg actgcatgac tcgcctgcac ctcccaagca ccgatgccct gtgtttgatc
     1201 gtaactgccg gaaacgctgt tcttgtcgac gacttcatga cgcagtccaa acgcgatgcg
     1261 cgtgttctcc acgatgtgca gttcgtttcg cacataggct gcgtgcgcgg tctgcgcggc
     1321 atcgctgtgg cccttgatgc gaccggtctg ccattgggaa aaatcgagtc ccgtcaccag
     1381 ttcatttttc agcacaccgc cttcgacgat ccggcgcaga cgtggcgaaa actgcgtagt
     1441 gcggctatgc acttcgttga ccgcgccgcc accgtaactg gagcgggaaa ttttttcgtg
     1501 atgcgacagt tccgccgccg cctcaagcgt gtcgaacctg cgttcgaaga ataccgtgac
     1561 acgatcactg tcgtaggcgc cgtgatccag aggcgtagcc gtctggcgtg ggttggcaag
     1621 gaattgcgcc atgctcagag cgccggcaag accatagtcg gagcgtgcca gatcgacacg
     1681 caggcccaag cgtcccaggc tggaaaacca ttgcgccccg ccgctgaaat ttttctggct
     1741 ggaggcattg ttgtcgcgat agttatccgt atgttgcctg ctgtaggcag tatgcagtgc
     1801 aaagccgtcc caacctttcg agacgatgga gcggccactg cgcaaaccat catcgcccac
     1861 ttcgacgatg accgtgccgt gtactgtgtc agccgtcggc tgcctggtga tgatccggat
     1921 cgtaccgccg gtggcgccgt caccatataa cacgctgccg ccgccgcgca cgatcttgat
     1981 gcgatcgacg gtatcgatcg gcacagaaga cagcaaggca gtcgccagct cgttttccga
     2041 caagcgcacg ccatcgacca gaatgaccag attctgattg ccgttactgc cgaagccacg
     2101 catatcgaga gaaaagtcac tggaacctgt cagactttga cggccatata cgccaccgag
     2161 tttacgaatc gcttcattga cgttatcgat gccggcatgg cgtatgtcat cggccatgat
     2221 caccgtggca cctatagttg cgaacgccgg atcattggga aagcgggacg ccgttacggc
     2281 aatcggcgtt aggttcccat ctgtcacggt gtgcgcggcg acgacggc
//
LOCUS       DASRDH010000105         2911 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1175139,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000105 DASRDH010000000
VERSION     DASRDH010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1175139"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="VFA14_02500"
     CDS             <1..260
                     /locus_tag="VFA14_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HZS80287.1"
                     /translation="MRHMNYQPWVGLPNILARTFLVPELLQNAATPQALADALWQQMN
                     DPAHVQRLRQRFTDMHHSLLRDTANVSAQAVCSLLEGKKTA"
     gene            257..916
                     /gene="rnhB"
                     /locus_tag="VFA14_02505"
     CDS             257..916
                     /gene="rnhB"
                     /locus_tag="VFA14_02505"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HZS80288.1"
                     /translation="MTKKNQPDASLLALGDADGETVCGVDEAGRGPLAGPVFAAAVIL
                     DPAQPIAGLRDSKKLSAASRDALALEIRERAIAWAIAACSEEEIDRLNILHASLLAMR
                     RAVEALQTVPTLALIDGNRCPLMRVRAQAIVKGDDKVPSISAASILAKTARDAALMVL
                     HAQYPHYAFDRHKGYPTVLHLERLRRYGVSPVHRRSYAPVRALLDAHSASAQDEAERY
                     A"
     gene            913..1746
                     /locus_tag="VFA14_02510"
     CDS             913..1746
                     /locus_tag="VFA14_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HZS80289.1"
                     /translation="MKSISSRDNPLYKELKRLATSAQARRKSRCSLLDGVHLAAACLQ
                     QAGAPQLCVVAESAVDHVEVVPILAQCETGGAPCISLPDVLYQALSQVENGVGLLLVI
                     ATPQPQAPQVLTTSAVLLDGLQDPGNLGSILRSAAAAGIRQVLCSGGTAAAWSPKVLR
                     AGMGAHFLLDIIEDADLLSLCRSSRIPVLATSSHAQKTIYQNDLTQEVAWLFGHEGQG
                     VADALLEAAGARVAIPHLGPVESLNVAAAAAVCFFEQARQRQDGLKNRASLPERVVPP
                     L"
     gene            complement(1801..2706)
                     /gene="rsgA"
                     /locus_tag="VFA14_02515"
     CDS             complement(1801..2706)
                     /gene="rsgA"
                     /locus_tag="VFA14_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007880294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HZS80290.1"
                     /translation="MSATENNTEAGTVIAAHGRHYLVQAGAEKLQCVTRGKKGDVVVG
                     DKVHLRRTSPNQAVIESIAGRQTLLYRSDQYKSKLLAANVTQLFIVTATEPGFSDDLV
                     SRALIAAESAGVDTHIILNKTDLAAALPKARARLQVYSKLGYPVHEVSALTQADATRN
                     AFLPLLQGQSTILIGQSGMGKSSLINLIVPDADIATREISAALDTGKHTTTFTRLYAM
                     SDDTAVIDSPGFQEFGLYQLSEGMLERAFREFTPHLGQCRFYNCHHLNEPGCAVLAAV
                     ESGKIAPMRHRLYAQLLHESSQKLY"
     gene            complement(2728..>2911)
                     /locus_tag="VFA14_02520"
     CDS             complement(2728..>2911)
                     /locus_tag="VFA14_02520"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017454021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HZS80291.1"
                     /translation="ADAFAAQHTNAQDLVSALVKLYEDNASTLTPDPLHSAFYDSHPP
                     ASLRIGKLHGRLNAAR"
BASE COUNT          535 a          818 c          925 g          633 t
ORIGIN      
        1 tcatgcgcca tatgaactat cagccctggg tcggtttgcc gaacattctg gcgcgcacat
       61 tcctggtgcc tgaattactg cagaatgccg ctacgccgca ggcgcttgct gatgccttat
      121 ggcagcagat gaacgatccg gcgcatgtgc aacgattgcg gcagcgtttc accgacatgc
      181 atcattcctt gttgcgtgat accgcgaatg tgagcgcgca ggcggtttgt agcctgctgg
      241 aaggaaaaaa aacggcatga caaagaaaaa ccaaccggat gcatcgttgc tggcgctggg
      301 cgatgccgac ggcgaaacgg tgtgcggcgt cgatgaagcc ggtcgtggtc cgctggcagg
      361 cccggtattc gctgccgccg tgattctcga tcccgcgcag ccgatcgccg gtctgcgcga
      421 ttctaagaag ctgagcgcgg ccagccgcga tgcgctggcg ctggagatca gggagcgtgc
      481 gatcgcctgg gccatcgctg cctgctcgga agaagaaatc gaccggctca atatcctgca
      541 cgccagtctg ctggccatgc gccgtgcggt cgaggccctg caaacggtgc cgacgctggc
      601 tctgatcgat ggcaatcgct gcccgctcat gcgcgttcgc gcacaagcca ttgtcaaggg
      661 tgacgacaag gtgccatcga tttccgctgc ttccattctt gccaagactg cgcgcgatgc
      721 ggcgttgatg gtactgcatg cgcagtatcc gcattacgct ttcgatcggc acaaggggta
      781 tccgacggtg ttgcatctgg agcgtctacg ccggtatggc gtctcgccag tgcatcgcag
      841 atcatatgcg ccggtacggg ccttgctgga tgcccactct gcatcggcac aggatgaagc
      901 agagcgatat gcatgaagtc catttcctcg cgcgacaacc cgctgtataa agagttgaaa
      961 cgactcgcca ccagcgccca ggcgcgccgc aagtcgaggt gcagtttgct cgatggcgtg
     1021 catctggccg cagcctgctt gcagcaggcc ggtgcgccgc agctgtgtgt ggtcgcggag
     1081 agcgctgttg atcatgtgga agtcgtgcct atcctggcgc agtgcgaaac aggcggcgcg
     1141 ccttgcatca gtctgccgga cgttttgtat caagccttga gccaggtcga gaatggtgtc
     1201 ggcctgctgc tcgtgattgc caccccgcaa ccacaggcgc cgcaggtatt gacgacatcg
     1261 gcagtgttgc tggatggttt gcaggatcct ggcaatctcg gttccattct gcgtagtgct
     1321 gctgctgccg gcatacgtca ggtgttgtgt tcgggcggta ctgctgctgc gtggtcgccg
     1381 aaagtactgc gcgccggcat gggagcgcat tttctgctcg acattatcga ggatgccgat
     1441 ttgctgagcc tttgccggag cagcaggatt ccggtgctgg cgaccagttc tcacgcgcaa
     1501 aagacgattt accagaatga tctcacgcag gaagtcgcgt ggctgttcgg tcatgagggt
     1561 cagggtgtcg ccgacgcatt gctggaagcg gccggcgcca gggtcgcgat tccgcatctg
     1621 gggccggtgg agtcgctcaa cgttgccgcg gcggcagcgg tctgcttctt tgaacaggcc
     1681 cggcaaaggc aggatggcct taaaaatcgc gcgagcttgc ccgaacgagt ggtgccgcct
     1741 ctgtaatcca gttttcacat aagtcgtcat tgccaatcca tctggcatca gcccagtatt
     1801 tcaatacagc ttctgcgacg attcatgcag caattgcgca tacaagcgat gtcgcatggg
     1861 cgcgatcttg ccgctttcga ccgctgccag tacagcgcag cccggttcgt tcagatgatg
     1921 gcagttataa aacctgcatt ggcccagatg cggcgtgaac tcgcggaagg cgcgttccag
     1981 catgccttcg ctgagctggt ataaaccgaa ttcctgaaat cctggcgagt cgatcacggc
     2041 agtatcgtcg ctcatcgcat acagacgcgt gaaagtggtg gtgtgcttgc cggtatcgag
     2101 cgcagccgag atttcgcgcg tggcgatatc ggcgtccggt acgatcaggt tgatcagcga
     2161 tgacttgccc atgccggact gaccgatcag gatggtcgac tggccttgca gcaagggcag
     2221 aaaagcattg cgtgtcgcat cggcctgcgt cagcgcggat acttcatgca ccgggtagcc
     2281 gagtttgctg taaacctgca gccgcgcgcg cgctttcggt agcgctgctg cgaggtcggt
     2341 tttgttgagg atgatatggg tatcgacgcc ggccgattcc gccgcgatca gggcgcgtga
     2401 caccaggtca tccgaaaacc ccggctcggt cgctgtcacg atgaataatt gggtaacgtt
     2461 ggcggccagc agcttggatt tatattggtc cgagcgatat agcagggtct ggcgcccggc
     2521 gatcgattcg atcacggctt gattgggcga ggtgcgcctg aggtggacct tgtcgccgac
     2581 tacgacatcg cctttcttgc cacgtgtgac gcattgcagt ttttccgcgc cagcctgtac
     2641 cagataatgg cggccatgcg cggcgatgac ggtgccggct tcggtgttgt tttcggtagc
     2701 gctcatgctc gggattgcgt gcaggcgcta gcgtgccgca ttcaatctgc cgtgcagttt
     2761 gccgattcgc agcgacgcgg gcggatgcga atcatagaat gccgagtgca acgggtccgg
     2821 cgtcagcgtc gaggcattgt cttcgtacag tttgaccagc gccgacacca gatcctgcgc
     2881 gttggtgtgt tgcgcggcaa atgcatcggc t
//
LOCUS       DASRDH010000106         3999 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1252539,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000106 DASRDH010000000
VERSION     DASRDH010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 3999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3999
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1252539"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..707
                     /locus_tag="VFA14_02525"
     CDS             <1..707
                     /locus_tag="VFA14_02525"
                     /inference="COORDINATES: protein motif:HMM:NF014686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HZS80292.1"
                     /translation="YEKLLNELKAIDSLEVVLAGCETGVKLADQLSEDLGLPSNGRLK
                     TEARRDKYAMAKALTSAGLRSAKTYLVDSLAELENALNELGLPVVVKPCASAGSDDVY
                     ICRTKDEVVDAFKRVHLKTNTLGLVNKGVLLQQLLLGRQYIVNSVSYAGKHFAVEIWE
                     DNREDAGSAYIYNYERLLLSYGQLEKDLVEYTKAVLNALGIRNGPCHVELMVNEGIPT
                     LIEVGARPAGGIERDH"
     gene            complement(1042..3771)
                     /gene="polA"
                     /locus_tag="VFA14_02530"
     CDS             complement(1042..3771)
                     /gene="polA"
                     /locus_tag="VFA14_02530"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HZS80293.1"
                     /translation="MQNTLLLVDGSSYLYRAFHALPDMRNAEGAPTGALYGIINMLRR
                     LRHDYPASYIACIFDAKGKTFRDNLYAEYKATRKSMPEDLVLQIEPIHAAVRALGWPI
                     LMVEGIEADDVIGTLGVRAAEAGLQTIISTGDKDMAQLVNDRVTLVNTMSNETMDRAG
                     VIARFGIPPERIVDYLSLVGDTVDNVPGVEKCGPKTAVKWLAQYDSLDGIMANADRIT
                     GVVGENLRKALPWLPQARMLVTVKTDCDLSAHMGTILDSLTVRPNADAALREIFTRYN
                     FRTWLRELDRAQETAVAATASQSGLFDTSTGLVKKYETILTEAQLDAWIATVNAAVMT
                     AVDTETTSLNPMQAQLVGISLCCEPGCAAYIPMAHNYQDMPTQLPRELVLARLKPWLE
                     DETRPKLGQNLKYDSHIFAGHGVQLRGIVHDTLLESYVLESHRTHDMDSLALRHLNRV
                     TISYTDVCGKGASQIGFDQVEIGRATEYAAEDADVTLALHQFMWPQIESDPRLRFIYE
                     KIEVPVSVVLQKIERNGVLIDAGILATQSSELGKRMLELEQHAYALAGQPFNLNSPKQ
                     LGEILFAKLQLPVVKKTPSGTPSTDEEVLQKLAGDYPLPKILLDYRGLAKLKSTYTDK
                     LPRMVDPATGRVHTNYAQAVAVTGRLASNDPNLQNIPIRTAEGRRIREAFIAPPGSMI
                     VSADYSQIELRIMAHISEDTGLLKAFADGEDIHRATAVEIFGVGLNDVTSEQRRYAKV
                     INFGLIYGMSTFGLASNLGIERAAAQMYIDRYFQRFSGVRQYMDDTRLKARSQGYVET
                     VFGRRLWLPEINSPNGPRRQGAERAAINAPMQGTAADLIKLAMIAVQDWIEHAGLQSR
                     LIMQVHDELVLEVPGGELDTVRERLPELMKNVAQLKVPLIADVGVGKNWDQAH"
     gene            3772..>3999
                     /locus_tag="VFA14_02535"
     CDS             3772..>3999
                     /locus_tag="VFA14_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007878978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HZS80294.1"
                     /translation="MEFRGKNIPRLREVDDIERATAKKARESWHVLTIMAEFVESAER
                     LSELRPAVSIFGSARLKTDDPYYALCIDIARR"
BASE COUNT          896 a         1047 c         1124 g          932 t
ORIGIN      
        1 attacgaaaa actgctcaat gaacttaagg caattgatag tcttgaggta gttcttgctg
       61 gctgcgagac tggagttaag ctggcggatc aactttctga ggatctcgga ctaccttcta
      121 acgggagact caagactgag gcaaggagag ataagtatgc gatggcgaag gctcttacat
      181 ctgccgggct taggtcagcc aagacatatc tggtagatag tctggctgag cttgaaaatg
      241 cacttaacga actcgggcta cctgtagttg taaagccatg cgcaagtgcc gggtctgatg
      301 acgtctatat ttgcagaacg aaagatgagg tggttgatgc attcaagcgt gtgcatctta
      361 agaccaacac cctaggcttg gttaacaagg gggtgttgct ccaacaactt cttcttggaa
      421 ggcaatatat agttaactct gttagctacg ctgggaagca tttcgccgtt gagatctggg
      481 aggataatag agaggacgct ggatcagcat atatatacaa ctacgaacga ctacttctca
      541 gctacggaca gctcgaaaaa gatttggttg agtatacgaa ggccgtgttg aacgctctag
      601 gcattcgtaa tggcccgtgc catgtagaac tgatggtcaa cgaaggcatt cctaccctaa
      661 ttgaggttgg tgccagacca gctggtggta ttgagcgcga ccattgattg ctttctaaat
      721 ggtggtttga ctattgattt tcagaataag gagaattgca aacatgtcat atctgtttct
      781 ttgatcagtc gacgctccgg aaaattgagg gaatatcggc gtcttgagac gatcaaagag
      841 tttgcttcat ttcaatgttt agtcgggctg cctacggtcg gcgatttcgt gccagtcaca
      901 agggatctag aaagctcgcc aggtatcttg atgttgtccc atgagtcgct gcaacaggtt
      961 cttgacgacc tggaggcgtt tagagtgatc aaactcaaag atctctttga ccttgtgtag
     1021 cctcctcgtc gatcacagct gtcaatgggc ttgatcccag tttttgccca cccccacgtc
     1081 ggcaatcagc ggtaccttca actgcgccac atttttcatc agttccggca gtctctcgcg
     1141 cacggtgtcc agctcgccgc cgggtacttc caggacaagt tcatcgtgta cctgcatgat
     1201 caacctggat tgcaggccag cgtgctcgat ccagtcctgc acggcgatca ttgccagttt
     1261 gatgagatcg gcggcagtac cctgcatggg cgcgttgatc gcagcgcgct cggcgccctg
     1321 acggcgcggg ccattgggcg aattgatttc cggcaaccac agacggcggc caaagacggt
     1381 ttcgacatag ccttgcgacc tggctttgag acgcgtgtca tccatgtact gtctgacgcc
     1441 ggagaaacgc tgaaaatatc tgtcgatata catctgtgca gcagcgcgtt cgataccgag
     1501 gttgctggcc aagccgaagg tgctcatgcc atagatcaaa ccgaagttga tcactttggc
     1561 atagcggcgc tgctcactag tgacgtcatt caggccgacg ccgaaaattt ccacagcggt
     1621 ggcgcggtga atgtcttcac cgtcagcgaa agccttgagc aggcccgtat cttcagagat
     1681 atgcgccatg atgcgcaact cgatctgtga ataatcggcg gaaacgatca tgctgcccgg
     1741 cggggcgata aaagcttcgc ggatacggcg gccttcggcg gtgcggatcg gaatattttg
     1801 cagattggga tcattggatg ccaaccggcc ggtgacggcg acggcctgcg cgtaattagt
     1861 gtgtacacgg ccggtagcgg gatcgaccat cctgggcagc ttgtcggtat acgtcgactt
     1921 cagtttggcc agcccacgat aatcgagcag gatcttcggt aacggataat ccccggccag
     1981 cttttgcaac acttcttcat cggtcgatgg cgtgcccgac ggggtcttct tgacgaccgg
     2041 caattgcagc ttcgcaaaca gaatttcgcc aagctgcttc ggtgaattga ggttgaacgg
     2101 ctggccggcc agcgcatagg catgctgttc gagctccaac atgcgcttgc cgagttcgct
     2161 ggattgcgtc gccagaatgc ctgcatcgat cagcacgcca ttgcgctcga tcttttgcag
     2221 cacgactgaa accggcactt caattttttc gtaaatgaaa cgcagtctgg ggtcgctttc
     2281 aatctgcggc cacatgaact gatgcaaggc cagggtgacg tcggcatctt cggcagcgta
     2341 ttcggtggcg cgaccgatct cgacctgatc gaaaccgatc tgcgacgcgc ccttgccgca
     2401 cacatcggta taactgatcg taacgcggtt gagatggcgt agcgcaaggc tatccatatc
     2461 gtgtgtgcga tgcgattcaa gaacgtagga ctccagcagc gtgtcatgca cgatgccgcg
     2521 caattgcacg ccatgaccgg cgaagatgtg actgtcgtat ttcaggtttt gcccaagctt
     2581 tggcctggtc tcgtcttcca gccagggctt gagcctggcg agcaccagtt cgcgcggcaa
     2641 ttgcgtgggc atatcctggt aattgtgcgc catcggaata taagcagcgc agccaggttc
     2701 gcaacacagc gaaataccga ccagttgcgc ctgcatgggg ttcagcgacg tggtctcagt
     2761 atcgacggca gtcataacgg ccgcgttgac tgtagcaatc caggcatcca actgcgcttc
     2821 ggtcaagatg gtctcgtatt tcttgacaag gccggtactg gtgtcgaaca gaccgctttg
     2881 cgatgccgtc gctgctacgg ctgtttcttg cgccctgtca agctcgcgca gccaggtacg
     2941 gaaattgtag cgagtgaaaa tttcccgcaa cgctgcgtca gcgttgggtc gtaccgtcaa
     3001 ggaatcaagg atagtaccca tatgtgccga cagatcacag tcagttttga cggtcaccag
     3061 catgcgcgcc tgcggcaacc atggcaaggc cttgcgcaag ttctcgccaa ccacgccagt
     3121 gattctgtca gcattggcca tgatgccgtc gagcgaatca tattgcgcaa gccatttgac
     3181 ggcagtcttg gggccgcact tctcgacgcc aggcacgtta tcgacagtgt caccaaccaa
     3241 gctcaggtaa tcgacgatgc gttctggtgg aataccgaat ctggcgatga caccggcgcg
     3301 atccatcgtt tcattgctca tggtattgac cagcgtgacg cggtcgttga ccagctgcgc
     3361 catgtccttg tcgccggtgg aaatgatggt ctggagtcca gcctcagcag cgcgcacgcc
     3421 gagcgtacca atgacgtcat cggcctcgat gccttcgacc atcagaatcg gccagcccaa
     3481 tgcgcgcacg gcggcgtgga tcggttcgat ctgcaagacc agatcctccg gcatcgattt
     3541 gcgtgtggct ttatactcgg cgtacaggtt gtcgcgaaac gtctttccct tggcgtcgaa
     3601 tatacaagcg atgtaacttg cgggataatc atgacgcagt cggcgcagca tattgatgat
     3661 gccgtacaac gcgccggtcg gcgcgccttc cgcgttacgc atgtcaggca aggcatgaaa
     3721 cgcccgatag aggtaactgg aaccatcaac taacaacaag gtgttctgca tatggaattc
     3781 agggggaaaa acataccgcg actgcgggaa gtggatgaca tcgagcgcgc caccgccaag
     3841 aaagcgcgcg agtcatggca tgtgctcacg attatggcag aattcgtcga gtccgccgag
     3901 cggctgtcgg agttgcgacc tgccgtttcc atcttcggtt ccgcgcgcct gaaaacagat
     3961 gatccgtatt atgccctgtg catcgatatc gcacggcgc
//
LOCUS       DASRDH010000107         3147 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_96586,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000107 DASRDH010000000
VERSION     DASRDH010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 3147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3147
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96586"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1201
                     /locus_tag="VFA14_02540"
     CDS             <1..1201
                     /locus_tag="VFA14_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HZS80295.1"
                     /translation="KVNEPLIHDGIAVYQSGFEDGGSKLRLTGYAMRDAEDRTFRIAG
                     EVHGNTTLLDMDGKDSGYTVEWTGFRPFNVENMGRSTSLDTLGVNKEPDESFAERLAS
                     RLGSGAGKAKDWKNVGPSVQYKLRDKAGQAREFLNYMMPVRIDGTEVFLAGVRDLPDE
                     PFRYLRIPADDDHSVIEWMRLRAALAHPALREEAAARYARLAMRSSSADTGQLREQVR
                     QSALRSLNIFAGDGKTSGYIAVSQFLETIPAAEQGKAADIFMKILNGSMWELWQAARE
                     QARLAPMATDEKHARFLQLAITALADAGFYLAPVYLQLSSFDEIKASVFQVTRAPGRK
                     VVYLGCLLLVLGVFSMLYIRERRLWIWIRPQGSETHALMAMSTQRKTLNFEKEFNLLK
                     VELTQTV"
     gene            1233..2384
                     /gene="ccsB"
                     /locus_tag="VFA14_02545"
     CDS             1233..2384
                     /gene="ccsB"
                     /locus_tag="VFA14_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HZS80296.1"
                     /translation="MESSQPYAYPQDIGFLKRLSVPDWIHAAVLVTAVLLALHRYGSH
                     MDHYEKALLALAAPTFAWLGWHWTQARRLMALLAVLALAAIAMYGGVPEMADRKFFLK
                     YFLSSQSAILWMSALFVLATLFYWLSLALRSETGAVIGSRLCWAAVALGFTGMLVRWY
                     ESYLIGADIGHIPVSNLYEVFILFCVITALFYLYYEERYATRQLGAFVLPVITAAVGF
                     LLWYTLARDAAGIQPLVPALQSWWMKIHVPANFIGYGAFALAAMVAMAYLFKSSGWLA
                     DRLPSLEVLDDVMYQAITVGFAFFTIATILGALWAAEAWGGYWSWDPKETWALIVWLN
                     YAAWLHMRLMKGLRGQLAAWWALAGLLVTGFAFLGVNLFLSGLHSYGEL"
     gene            2384..>3147
                     /locus_tag="VFA14_02550"
     CDS             2384..>3147
                     /locus_tag="VFA14_02550"
                     /EC_number="3.1.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HZS80297.1"
                     /translation="MSTPIQVAFETPMQLGECPLWHANEGMLYWIDIPDQAVHCLHEA
                     SRKHYSWAMPCEPGCIAMHADGGLLVALRSGLVRLNTSTGAISPLIDAPYDTSSLRFN
                     DGRCDAMGRFWSGTIYEPRDRELGSLYCFERGTLRNAAHPVTTSNGVAFSKDQRTMFH
                     ADTPAHRIDAYDFDPATGKTSNRRLFKQFNMDKMAVNYGGRPDGAAVDSENTYWCAMF
                     EGGRILRLSAAGDILQEIALPVRCPTMVAFGGRDLKT"
BASE COUNT          628 a          889 c          959 g          671 t
ORIGIN      
        1 caaggtcaac gaaccgctga tccatgacgg catcgcggtg tatcagtccg gtttcgagga
       61 tggcggcagc aagttgcgcc tgaccggata tgcgatgcgc gacgccgagg acagaacctt
      121 ccggatcgcc ggcgaagtcc acggcaacac aacactgctg gacatggacg gcaaggacag
      181 cggctatacc gtggaatgga ccggctttcg ccccttcaat gtcgaaaaca tgggccgcag
      241 caccagcctg gatacgctcg gtgtcaacaa agaaccagac gaatccttcg ccgagagact
      301 tgccagtcgt ctcgggtccg gcgccggcaa ggccaaggac tggaaaaacg ttggtccgag
      361 tgtgcagtac aagctgcgcg acaaagcagg ccaggcgcgc gaatttctga attacatgat
      421 gccggtgcgg atcgacggca ccgaggtttt cctggccggc gtgcgcgatc tgccggacga
      481 accgttccgt tatctgcgca tccctgccga tgacgaccac agcgtcatcg aatggatgcg
      541 tctgcgcgcc gcgctggcgc atccggccct gcgcgaagaa gccgccgccc gctatgcgcg
      601 cctggccatg agaagcagca gcgccgatac cggacagctg cgtgaacagg tgcgacaatc
      661 ggccttacgt agcctgaaca ttttcgccgg cgacggcaag acgtcgggtt atatcgccgt
      721 atcacagttt cttgaaacga tcccggctgc cgagcagggc aaagccgccg atattttcat
      781 gaaaattctc aacggcagca tgtgggaact gtggcaggct gcgcgcgagc aagcgcgact
      841 cgcgccaatg gcgaccgatg aaaaacacgc acgctttctg caactagcga tcactgccct
      901 ggccgatgct ggtttttatc ttgcgccagt gtatctgcaa ctgagcagtt tcgatgaaat
      961 caaggcatcg gtattccagg tgacgcgcgc gccgggcagg aaagtggtgt atctgggctg
     1021 tctgctcctg gtgctgggcg tgttctccat gctatacatc cgggaacgcc gcttatggat
     1081 ctggatacgt cctcaaggaa gcgagacaca tgcgctgatg gccatgagta cgcagcgcaa
     1141 gacgctgaat ttcgagaaag aattcaacct gctcaaagtg gaactgacgc aaacggtgta
     1201 agctgacgcc cgaagaccga tcggagaaaa tgatggaatc gagccaacct tacgcatacc
     1261 cgcaggatat cggctttttg aagcgcctga gcgtgccgga ctggatccat gccgccgtgc
     1321 tggtcacggc tgtgctgtta gcactgcacc gctacggcag ccatatggat cattacgaaa
     1381 aagcgctgct ggcgctggcg gcaccgacct ttgcctggct cggctggcac tggacgcagg
     1441 cgcgccgact gatggccttg ctggcggtgc tggcgctggc ggcgatcgcg atgtatggcg
     1501 gtgtaccgga gatggccgac aggaaatttt tcctgaaata tttcctctcc agccagtcgg
     1561 caatcctatg gatgagcgca ttgtttgtgc tggcgacgct gttctactgg ctcagtctag
     1621 cgttgcgttc ggagacaggc gcggtgatcg gttccaggct gtgttgggcg gcggtggcgc
     1681 tgggttttac cggcatgctg gtgcgctggt acgagtccta tctgatcggc gccgacatcg
     1741 gccacattcc ggtctccaat ctgtatgaag tattcattct gttctgcgtg atcacggcgc
     1801 tgttttatct gtattacgaa gaacgctatg cgacgcggca gcttggcgca ttcgtactgc
     1861 cagtgatcac ggcggcggtc ggtttcctgt tgtggtacac gctcgcgcgt gacgccgccg
     1921 ggatccagcc gctggtgccg gcactgcaaa gctggtggat gaagatccat gtgccggcca
     1981 atttcatcgg ttacggcgcc ttcgcgctgg ccgccatggt ggcaatggcc tatctgttta
     2041 agtccagcgg ctggctggct gatcgcctgc cgtcgctgga agtgctcgac gatgtcatgt
     2101 accaggccat cacggtcggc ttcgctttct tcaccatcgc caccattctc ggcgccttgt
     2161 gggcggcgga agcctggggc ggttactggt cttgggatcc aaaagaaacc tgggcgctga
     2221 tcgtctggct caactatgca gcatggctgc acatgcgcct gatgaaaggg ttacgcggac
     2281 agcttgccgc ctggtgggcg ctggccggtt tgctggtgac gggctttgcc ttcctcggcg
     2341 tgaacctgtt tctgtccggc ttacactctt acggtgaact gtaatgtcta ctcccataca
     2401 ggtcgcattc gagacgccca tgcagttggg cgaatgtccg ctttggcatg ctaatgaagg
     2461 tatgctttac tggatcgata ttcccgacca ggcagtacat tgcctgcacg aagccagcag
     2521 gaaacactac agttgggcga tgccttgtga acccggctgt atcgccatgc atgctgatgg
     2581 tggtttgctg gtggccttgc gcagcggcct cgtgcggctg aataccagca cgggagcaat
     2641 ctcgccgctc atcgatgcgc cttacgacac cagcagctta cgtttcaacg atggtcgctg
     2701 cgatgccatg gggcgcttct ggagtggcac gatttacgaa ccgcgcgatc gcgaactagg
     2761 cagcctttat tgcttcgaac gcggcacact acgcaatgca gcgcacccgg taacgacatc
     2821 gaacggcgtc gcattcagca aggatcagcg cacgatgttc cacgccgata caccagcgca
     2881 tcgcatcgat gcttatgact ttgatcctgc caccggaaaa accagcaacc ggcgtttgtt
     2941 caaacagttc aatatggaca agatggctgt gaattatggc ggtcgccccg atggcgccgc
     3001 ggtcgatagc gagaatacgt attggtgcgc aatgttcgag ggcggcagaa tattgcgcct
     3061 ctctgccgcc ggagacatat tgcaggagat tgcgcttccg gtgcgctgcc cgaccatggt
     3121 cgctttcggt ggccgtgatc tgaaaac
//
LOCUS       DASRDH010000108         2970 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_464373,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000108 DASRDH010000000
VERSION     DASRDH010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2970
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_464373"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            324..998
                     /locus_tag="VFA14_02555"
     CDS             324..998
                     /locus_tag="VFA14_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017454421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZS80298.1"
                     /translation="MNAGSLSETLPIQVGIADDYPVVLRGVESILEHCHDIVVTFSVE
                     SVALLLKQLEEAGPVDVLLCDYAFDDTPQADGLNLLRKIKRIAPSTHVLFLSAHAVPH
                     IISGVLELGAAGFIGKSKTDFANLPQAIRMVQSGHIYLPSSLSSLLLEQVFWKKNQAV
                     RLEMLSERELVVVRMICEGFSVAAIAECLHRSPKTVSNQKLSAMKKLGANNDIELSRI
                     FRDLSS"
     gene            1011..>2970
                     /locus_tag="VFA14_02560"
     CDS             1011..>2970
                     /locus_tag="VFA14_02560"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HZS80299.1"
                     /translation="MQILALSGQYYRRMLYGCAVFLATTILSVSVFSGFSAFSQFRER
                     QIGVFVGKREQIKSEVDRLFVRVIQFAEMYGHLRLQMSYEKEVPLLLSDRKIFNENDG
                     ISLRHPNLTGAPFSLVTRKNASRNDEEQAGLLLLLKLASSLPMFNPLDPGVTLDGFIY
                     TSDKSWLAISPPLTGKERVSSRSLGVQEFMESISASVDAAFITHSLSAANVQGSTGQS
                     VIWMTPSDSKTFWREAMTQLAVRISLGDDNTATAVFSISPGQFKQFFMRNDHTPGLFV
                     FDSSATRAFFSGADDDVSRSQMSKIGENAARITADGDQINYFHRNGTFFVAQKIDGPG
                     WNIVYVFNWQDILAGLRGDLITGALWTSFLLLLVWVATIYSDKYIIGALQKRAFALIE
                     TRQFSQSIIDTLPVGIAVYAPDTGAVMLQNAVAVQMFESADIPSAGFYARIVSTSRFS
                     EPVLATSYGCAMIETELPQRGGGVNYLGVAYSTTRFSGQDVVLLGLIDMNTHKAHEAL
                     LLDAKKSADQANQEKSMFLAQVSHEIRTPLHGVIGHMELLSHEVLTPEQHRRIEMIRR
                     SLDTLMSLVNDILDVTKIESRAIHLNVAPMNLNDVLEECAQLFAPLILSKKLQFCCFP
                     DAALDLPVLGDGKRLMQILQNLVGNAVKF"
BASE COUNT          664 a          718 c          816 g          772 t
ORIGIN      
        1 gaggccctcg ggttccggag caacgatcca tcacacacgt gtgattaccg cttcgctgga
       61 aaaacttccg ggagaggtga ttacaccggg cagagtctat gcgattgctc gtgtcatcgt
      121 gaaagtccaa tgaaaacaaa cagtaacgga ggcagcgagg ggaagtagct ttctgtctac
      181 tcttttccac gattttcggg gtgattcagt tttgttgcaa aagcaacagc ttttgaaagc
      241 ttttgcggat gatcgcatga aattcgcgat attcttgttt tgttcttgtt tgggaacatt
      301 cttgcgattt tataggatca aggatgaatg caggcagttt gagcgagaca ttgccgattc
      361 aggtgggcat cgcagacgat tatcccgtag tgcttcgcgg cgttgagagt attctggaac
      421 actgccatga tattgtcgtc accttttccg tcgagtcggt tgcgctgttg ctcaaacagt
      481 tggaggaggc agggcctgtc gatgtgcttc tctgtgatta tgcattcgat gacactcctc
      541 aggcagatgg cctgaacctg ctgagaaaga tcaagcgcat cgcgccatcg acacatgtgc
      601 tgttcctgag cgcgcatgcc gtgccgcata ttatttcggg cgtgctggaa ttgggtgccg
      661 caggtttcat cggcaagagc aaaaccgatt tcgccaattt gccgcaagcc atccggatgg
      721 tccaaagcgg gcatatttac ctcccgtctt cgctgtcttc cctgttgctg gagcaggtgt
      781 tctggaagaa aaaccaggct gtcaggctgg agatgctttc ggagagggag ctggtcgtcg
      841 tgaggatgat ttgcgaaggt ttttccgtcg ccgcaatagc cgaatgcctg catcgcagtc
      901 ccaagaccgt cagcaaccag aaattgtctg caatgaaaaa gctgggggcg aacaacgata
      961 tcgaactcag tcgtattttt cgtgatttgt cttcctgaaa acgtataccg atgcaaattc
     1021 tggcgttgtc cgggcagtat tacagacgca tgctgtatgg atgcgctgtt tttcttgcga
     1081 caaccatcct gagcgtttcg gttttttcag gattcagtgc tttttcccag tttcgtgagc
     1141 ggcagatcgg tgttttcgtc ggcaagcggg aacagatcaa gtcagaggta gatcgcctgt
     1201 ttgttcgcgt cattcagttc gcagaaatgt atggtcattt gcgtctgcaa atgtcttatg
     1261 agaaggaggt gccgttgctt ctttccgacc ggaagatatt caatgagaac gatggtattt
     1321 cgcttcgcca cccaaatttg acaggggcgc cgttctcgct tgtcaccagg aagaatgctt
     1381 cccgcaacga tgaggagcag gctggcttac tgttgctgct gaagctggcg tcgtcgctgc
     1441 caatgttcaa tccgctcgat cctggcgtga cgctggacgg tttcatctat acatcagata
     1501 agtcctggct cgccatttca ccaccattga ccggaaaaga gcgcgtgtcg tcccgtagcc
     1561 ttggtgttca ggaattcatg gaaagtatca gtgcatcggt cgatgcggca ttcatcacgc
     1621 atagcttgtc tgccgcaaac gtgcagggca gtacaggcca aagcgtcata tggatgacgc
     1681 cgtcagattc gaagacgttt tggcgcgagg cgatgactca gctcgctgta cggatttctc
     1741 tcggtgacga taatacggcg actgccgtat tttccatatc gccaggccag ttcaagcaat
     1801 ttttcatgag gaacgatcat acgcccggac tctttgtgtt tgacagttct gcgaccaggg
     1861 catttttctc aggggcggat gacgacgtat cgcgtagcca gatgtcaaaa atcggggaga
     1921 atgccgcacg cattaccgcg gatggtgacc agatcaatta ttttcaccgg aacggtactt
     1981 tctttgtcgc ccagaaaatc gacggccctg ggtggaatat cgtgtacgtg ttcaactggc
     2041 aggatattct cgccggtctg cgtggcgatc tcattacggg agcgctctgg acctcgtttt
     2101 tattgcttct cgtatgggtt gcgacgatct attctgataa atacatcatt ggcgcgctgc
     2161 aaaaaagggc cttcgcattg atagaaacca ggcaattcag ccaatcgatc attgacacgc
     2221 taccggtggg tattgccgtc tatgcgccgg acaccggtgc ggtgatgctg caaaacgccg
     2281 tcgccgtaca gatgtttgaa tctgctgata tacccagtgc cggtttttat gcccgtattg
     2341 tcagtaccag caggttttcg gagccagtac tggcaacttc ctatggatgc gcgatgatcg
     2401 aaactgagct tccccaacgc ggcggcggag tcaactatct gggtgtggcg tactcgacaa
     2461 cgaggttttc cgggcaggat gtcgtgctgt tgggattgat cgacatgaat acgcacaagg
     2521 ctcatgaagc tttgctgctc gatgccaaga aatccgccga tcaggccaat caggaaaaat
     2581 cgatgttcct ggcccaggtg agccatgaaa tccgcacgcc gctgcacggc gtcattggtc
     2641 acatggaact actgtcacat gaagtgctga ctcccgagca gcatcggcgg atcgagatga
     2701 tacggcgctc cctggacaca ttgatgagtc tggtcaacga tatcttggat gtgacgaaaa
     2761 ttgaatccag agccatccat ctcaacgttg caccgatgaa tctcaacgat gtgctggaag
     2821 agtgcgcgca gctttttgcg ccgctcatcc tgagcaaaaa actgcaattt tgttgtttcc
     2881 cggacgctgc tctggatctg ccggtgctag gcgacggaaa gcggctcatg cagattctgc
     2941 agaatcttgt cggcaatgcg gtgaaattta
//
LOCUS       DASRDH010000109         2176 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_727844,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000109 DASRDH010000000
VERSION     DASRDH010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_727844"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            53..1027
                     /gene="denD"
                     /locus_tag="VFA14_02565"
     CDS             53..1027
                     /gene="denD"
                     /locus_tag="VFA14_02565"
                     /EC_number="1.1.1.410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-erythronate dehydrogenase"
                     /protein_id="HZS80300.1"
                     /translation="MHIVITGGAGFLGSRLARQLLQRGQLSGSDGKQRTITRITLLDM
                     VAAQDFNDARIESVVGDIADAAVIERTIGKDTQSIFHLAAIVSSHAEADFELGMKINF
                     DATRRILERARALGTRPRVVFTSSVAVFGGALPAQVLDDTLLMPQSSYGAQKAMGELL
                     VNDYSRKGFIDGRALRMPTISVRPGVPNQAASSFASGIIREPLNGQPSVCPVAPETRM
                     WLMSPRKAVDNLIHGHEINGADLGLTRFLSIDGLSVTVRQMVDALEQVTGANAVKLIT
                     WKEDDAIKRIVNSWPGNFAARRAGALGFTADSDFTSIVKAYIEDEMKK"
     gene            1107..2072
                     /locus_tag="VFA14_02570"
     CDS             1107..2072
                     /locus_tag="VFA14_02570"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HZS80301.1"
                     /translation="MQMLRQRTRSLSYLLLTLAVFGTLGGCASLTPQSDAVKHDTLRP
                     RSFTFKDGGQALYYSFTVGAPSAESALIFFVSGSGCASVKRRFPGYFDPIRHQLDARV
                     LVLQKRGIEENSHGNICPRAFTLTDYFDQTVADQTEFINTQLAMQAALPKTIVLMGAS
                     EGAIVAAKIASVDARMTHLALIGSGGSTVRENLQLLSQKLRYLRTPEKKFAAIAADPG
                     STTQSAWGHSYKYWSSLLDVDIGNLLLSLDIPIAMAMGEQDKNVPIETAYALKQKFVE
                     RKKQNFHLYAFPDADHHLYDRARTLSHVKEFLEGLVAQIHQEKAR"
BASE COUNT          498 a          632 c          580 g          466 t
ORIGIN      
        1 gcaaagttgt atgataactt tgcgcattca tccgcctcat ccaggagaat acatgcatat
       61 cgtcatcacc ggcggcgccg gttttctggg cagccgtctg gcgcgccaat tactccagcg
      121 cggtcagttg agcggcagtg acggcaaaca gcgcaccatc acccgcatta ccctgctcga
      181 catggttgct gctcaggatt tcaatgatgc gcgcatcgaa tcagtggttg gcgatatcgc
      241 cgatgcagcc gtgatcgaac gcaccatcgg caaagacacg caatccatct tccacctcgc
      301 cgccatcgtc agcagccatg ccgaagccga tttcgaactt ggcatgaaga tcaatttcga
      361 tgccacgcgc cgcattctgg aacgcgcccg cgcgctcggc accaggccgc gcgtggtatt
      421 tactagctcg gtcgcggttt tcggcggcgc gctaccggcg caagtgctgg atgataccct
      481 gctgatgccg caaagttcct atggtgcgca aaaggcgatg ggcgaactgc tggtcaacga
      541 ttacagccgc aagggcttca tcgatggtcg cgcgctgcgc atgccgacca tttccgtgcg
      601 ccccggcgtg ccgaaccagg cggcatcgag cttcgccagc ggtatcatcc gcgaaccgct
      661 caacggccag ccctcggtct gcccggttgc gcctgaaaca cgcatgtggc taatgtcacc
      721 gcgcaaggcc gtcgacaacc tgatccatgg ccatgaaatc aacggcgccg acctcggcct
      781 gacgcgcttt ctcagcatcg acggcctgtc agttacggtg cgtcagatgg tcgacgcgct
      841 ggaacaggtt accggcgcta atgccgtcaa gctgatcacg tggaaagaag atgacgccat
      901 caagcgcatc gtcaattcct ggccaggtaa cttcgcggcc aggcgcgccg gggcgctggg
      961 ctttactgcc gacagcgact tcaccagtat cgtgaaagcc tatatcgaag atgaaatgaa
     1021 aaaataatcc tgtatcttgc ataggggcag tccgtatcat taccgtcccc ttcgggagtc
     1081 ctcaattgca aagagttctc ttcacaatgc aaatgttgag acagcgcacc cgctcgcttt
     1141 cgtatttgct tttgacactc gccgtttttg ggacgctggg aggctgcgcg tcgcttactc
     1201 cccaatcaga tgcggtgaaa cacgatacac ttcgcccgcg ttcattcaca ttcaaggatg
     1261 gcggacaagc tctctattat tccttcactg ttggcgcgcc atctgctgag agcgcgctga
     1321 ttttcttcgt gagcggatcg ggatgcgcca gcgtgaagag gcgcttcccc ggctatttcg
     1381 atcccatacg acatcagctc gatgcccgtg tgcttgtgct gcaaaagcgg ggcatcgagg
     1441 aaaactcaca tggaaatatc tgcccgcgcg cgttcaccct gacagattat tttgatcaaa
     1501 cagtcgcgga tcaaacagaa ttcatcaata cgcaactggc catgcaggct gcgctgccaa
     1561 agacaattgt gctgatgggc gcatcggagg gggcaatcgt tgctgcaaaa attgcttccg
     1621 tggatgcgcg catgacacat ctcgccctga ttggtagtgg cggctcgacc gtgcgggaga
     1681 atcttcagct gctctcgcag aagcttcgat acttgcgcac accggaaaag aaattcgcgg
     1741 caattgctgc tgacccgggc agcaccacgc aaagcgcctg gggtcatagc tataaatatt
     1801 ggtcatcgct cctggatgtc gatattggca atcttttact ttcactcgat attcccatcg
     1861 cgatggcgat gggtgaacag gacaaaaacg ttcccatcga gaccgcctat gcgctcaaac
     1921 aaaaattcgt ggagcggaag aaacaaaatt ttcacttata tgcgtttccc gatgcggacc
     1981 atcacctcta tgaccgcgcg cgtaccctgt cgcatgtcaa ggaatttctg gaggggctag
     2041 tagcgcagat tcaccaagag aaagcacgat gaggcggcat gggaatacca aatcaagccc
     2101 agcttagaac acatcgaggt actgccgccg ccaaatcagc ggcaaacttc cgaaaacatc
     2161 tataatagag cgcttt
//
LOCUS       DASRDH010000110         5964 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_383717,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000110 DASRDH010000000
VERSION     DASRDH010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 5964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5964
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383717"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..712)
                     /locus_tag="VFA14_02575"
     CDS             complement(<1..712)
                     /locus_tag="VFA14_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007882671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HZS80302.1"
                     /translation="MPATVPSVLLDLCATADPSWHTILKAGLQAIAAADAAYLPVLAQ
                     DTAYLPTEGRLFAAFRQPMTAVRYVLVGEGPYPRAASATGMCFMDGVVGSLWSDKGLS
                     RQVNRATSLRNFMKMLLVADGQIHADKTSGDVLADIAAKAQADQSGMIQTLADLQDKM
                     TAQGFLLLNASLVFRSHVAPAKDARAWRPFFETILQGLADYAEACNKPLPTLVLWGKI
                     AGQLDALPVVARFPHAVSE"
     gene            complement(734..2824)
                     /gene="uvrB"
                     /locus_tag="VFA14_02580"
     CDS             complement(734..2824)
                     /gene="uvrB"
                     /locus_tag="VFA14_02580"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006712849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HZS80303.1"
                     /translation="MADLSPVSMASSALDESRLFRFPGSPYRLYQPFPPAGDQPVAID
                     KLVEGVNDGLFFQTLLGVTGSGKTYTMANTIARLGRPAIVFAPNKTLAAQLYSEFREF
                     FPQNAVEYFVSYYDYYQPEAYVPQRDLFIEKDSSINEHIEQMRLSCTKSLMERRDVVI
                     VATVSAIYGIGNPNEYHQMILTLRARDKVSQRDVIARLIQMQYTRNETDFGRGTFRVR
                     GDTIDVFPAEHAELALRIEMFDDEIDSLQLFDPLTGRVRQKIPRFTVYPGSHYVTPRA
                     TVLRAIDTIKDELRERLAFFRKENKLIEEQRLEQRTRFDIEMMTEIGFTKGIENYSRH
                     LSGAKAGEPPPTLVDYLPQDAIMFLDESHVLIGQLNGMYNGDRARKTNLVDYGFRLPS
                     ALDNRPLKFDEFQSKMRQTIFVSATPAEYENSHADQVVEQVVRPTGLVDPVISVRPAT
                     TQVDDLMSEISARVKKNERVLVTTLTKRMAEQLTEFLGDNGIKVRYLHSDIDTVERVE
                     IIRDLRLGIFDVLVGINLLREGLDIPEVSLVAILDADKEGFLRSERSLIQTIGRAARN
                     LNGMAILYGDRITGSMQKAIGETERRRVKQITFNEVNGITPMGIKKEVRELIDGVYSP
                     QEARLGLMVAQEQADYEAMSEKQVSKEIKRLEKRMQDHAKNLEFEQAAKVRDQLHQLK
                     QLLFGAPGEDTAVA"
     gene            2951..4168
                     /locus_tag="VFA14_02585"
     CDS             2951..4168
                     /locus_tag="VFA14_02585"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid aminotransferase"
                     /protein_id="HZS80304.1"
                     /translation="MNAPLSASLFTAIEMAPRDPILGITEAFNADRNPGKTNLGVGVY
                     YDDNGKVPLLECVRKAEAELSARSAPRTYLPIDGLADYDKAVQELVFGAGSAVIQEKR
                     AITVQALGGTGALKLGADFIKRFSPASTQVWISDPSWENHRALFEMAGFTVNNYPYYD
                     AATRGVDFTGMLAALQTMPAGSVVLLHACCHNPTGADLTADQWTQVIDVITSRSLLPF
                     LDMAYQGFGDGIEEDGRVVRRFAEAGGPLFVSNSFSKSFSLYGERVGALSIIAASSDE
                     AARVLSQLKRVVRTNYSNPPIHGGQIVATVLAVPELRILWEKELAEMRVRIREMRQLL
                     VTRLKEKASSHCFDFVIKQRGMFSYSGLTRAQVERLRTEFSVYAVDTGRICVAALNTK
                     NIDAVADAIAKVL"
     gene            4220..4295
                     /locus_tag="VFA14_02590"
     tRNA            4220..4295
                     /locus_tag="VFA14_02590"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4253..4255,aa:Asn,seq:gtt)
     gene            complement(4449..5180)
                     /locus_tag="VFA14_02595"
     CDS             complement(4449..5180)
                     /locus_tag="VFA14_02595"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HZS80305.1"
                     /translation="MNLPRFYCPGPFAIGTTHALPDQVAHHIQVIRMRVGNAMTLFNG
                     RGGEYAAVITRMEKRRVYAEIQTFSPREAELSYAVTLAQALPEAAKMDWIIEKAIELG
                     VTVIQPLAAQRCVVRFNSERAEKKQSHWQGIICAAAEQSGRNRLAQLSPLSDFRHWIA
                     QSDPHRRILLSPRGEQSLSDWTRHHSAQALSLLIGPEGGFSEAEEKLACDHGALILSM
                     GPRVLRAETAGLCALAAINAIWGEM"
     gene            5363..>5964
                     /locus_tag="VFA14_02600"
     CDS             5363..>5964
                     /locus_tag="VFA14_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013233434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HZS80306.1"
                     /translation="MISMLPTDKMANAIRALAMDAVQKANSGHPGMPMGMAEIAVALW
                     AKHYRHNPANPQWANRDRFVLSNGHGSMLQYALLHLTGYDLSMDDIRNFRQLHSKTAG
                     HPEFHITPGVETTTGPLGQGIANAVGMALAEKLLAAEFNKPGLAVVDHYTYVFVGDGC
                     LMEGISHEACSLAGALRLSKLIVLYDDNGISIDGHVEGWF"
BASE COUNT         1284 a         1683 c         1668 g         1329 t
ORIGIN      
        1 gctccgacac cgcgtgcgga aaacgtgcaa cgactggtaa ggcatcaagt tgtccggcga
       61 ttttccccca cagcaccaat gtcggtagcg gtttattgca tgcctcggca tagtctgcca
      121 gtccctgcaa tatcgtttcg aagaaaggtc tccaggccct ggcatctttt gccggcgcga
      181 cgtggctgcg gaataccagg ctggcattga gcagcaagaa tccttgcgcc gtcatcttgt
      241 cctgtaagtc ggccaaggtc tggatcatgc cgctctggtc ggcttgcgcc ttggcggcga
      301 tgtcggccag aacgtcgccg ctggtcttgt cagcgtgtat ctggccgtcg gcgaccaaca
      361 gcatcttcat gaagttgcgc agtgatgtgg cgcgattgac ctgtctggac aaacccttgt
      421 ccgaccatag cgagccgacc acgccatcca tgaaacacat gccggtggcg ctggctgcgc
      481 gcggatacgg gccttcaccg accagcacat aacgcactgc tgtcatcggc tgtctgaaag
      541 cagcaaaaag acgaccttcg gtgggcagat aagcagtatc ttgcgccagc acgggaaggt
      601 atgcggcgtc ggctgcggcg atggcttgca ggccagcttt caaaatggtg tgccaggaag
      661 gatccgcagt cgcacagaga tcgagcagaa cggaaggaac ggtggctggc atgaacatat
      721 gattgcttga atatcaagcg acggcggtat cttcgccggg cgcgccgaac agcaattgtt
      781 tgagttgatg caattggtcg cgcaccttgg cagcttgttc aaactccaga ttcttggcgt
      841 gatcctgcat acgcttttcc agacgcttga tttccttgct gacctgtttt tcgctcatgg
      901 cttcgtagtc ggcctgctcc tgcgctacca tgagtcctag gcgcgcttcc tgtgggctgt
      961 agacgccgtc gatcagttca cgaacttctt ttttgatgcc catcggcgta atgccgttaa
     1021 cttcattgaa agtgatctgc tttacgcgac ggcgttcggt ttcaccgatc gctttctgca
     1081 tcgaaccggt gattctgtca ccgtacagaa tcgccatacc attgaggtta cgcgcagcgc
     1141 ggccgatggt ctgaatcaga ctgcgctcgg agcgtaaaaa accctccttg tcggcatcga
     1201 ggatcgctac cagcgatact tcaggaatgt ccagaccttc gcgcaacaag ttgatcccga
     1261 ccaatacatc aaagatgccg aggcgcaggt cacgaatgat ttcgacgcgc tcgacggtat
     1321 cgatgtcact atgcaaataa cgcaccttga tgccattgtc gccgaggaac tcggtcaatt
     1381 gctcggccat acgcttggtc agcgtagtca ccagcacgcg ctcgttcttt ttgacgcgcg
     1441 cgctgatctc agacatcaag tcatcgactt gagtcgtcgc cggacgcacc gagatgacag
     1501 gatcgaccag gccggtcggg cgcaccactt gttcgacgac ctgatcggcg tggctgtttt
     1561 cgtattccgc cggcgtcgcc gatacaaaaa tggtctggcg catcttgctc tggaattcat
     1621 cgaacttcag cggccgattg tccagcgccg acggcaggcg aaagccataa tcgaccagat
     1681 tggttttgcg cgcacggtcg ccgttgtaca tgccgttcaa ctggccgatc agaacgtgcg
     1741 attcatcgag aaacatgatg gcgtcctgcg gcagatagtc gaccagcgtc ggcggcggtt
     1801 cgccggcctt ggcgccggac aggtggcgcg aatagttttc gatgcctttg gtgaagccga
     1861 tttcagtcat catttcgatg tcgaagcggg tgcgctgctc caaccgttgc tcttcgatca
     1921 gcttgttttc cttgcggaag aaggccagac gctcgcgcaa ttcatctttg atagtatcga
     1981 tggcgcgcag cacggtagca cgaggcgtga cgtaatgcga gccggggtag acggtgaagc
     2041 gtggaatctt ctggcgtaca cggccggtca gcggatcgaa taattgcagg ctatcgattt
     2101 cgtcatcgaa catttcgatg cgcagcgcca gttcagcatg ctcggccgga aacacatcga
     2161 tggtatcgcc gcgcacgcga aaggtgccgc ggccgaaatc ggtttcgtta cgcgtgtatt
     2221 gcatctggat cagacgcgca atgacatcgc gctgactgac cttgtctctg gcgcgcagag
     2281 tcagaatcat ttgatggtat tcgttggggt taccgatacc atagattgct gacaccgttg
     2341 ccacgatgac gacatcacgc cgctccatga gcgacttggt acaggacagg cgcatctgct
     2401 cgatgtgttc gttgatcgaa gaatctttct cgatgaacaa atcgcgctgt ggcacatagg
     2461 cttccggctg gtaatagtcg tagtagctga cgaaatattc gaccgcgttt tgcggaaaaa
     2521 actcacggaa ttcgctatat aactgcgccg ccagcgtttt gttcggcgcg aagacgatcg
     2581 ccggccgacc gaggcgggca atagtgttgg ccatcgtata agtcttgccc gagccggtca
     2641 cgcccagcaa ggtctggaag aacagaccat cgttgacgcc ttcgaccagt ttgtcgatcg
     2701 caaccggctg gtcgccggca ggtgggaagg gctgatataa acgataaggg gaacccggaa
     2761 acctaaacaa cctgctttcg tcaagcgcgg aagatgccat ggaaacgggg gataaatcag
     2821 ccattgtcat gcgacctttg ataaaatcat gcattgcaaa acagtgcccg gcgcacggct
     2881 ttgccctgca tccatcccga ctgcaatcaa tcaccaacat tggtggcagc cagcttttta
     2941 tgttatcacg atgaacgcac ctctctcagc ctctctcttc accgccatcg aaatggcgcc
     3001 acgcgatcct attctcggca ttaccgaagc tttcaatgcc gacaggaatc caggcaagac
     3061 caatctcggc gttggcgtct actatgacga taatggcaaa gtgccgctgc tggaatgcgt
     3121 gcgcaaggcg gaagctgaac tgtcagccag atccgctccg cgcacctatt tgccaatcga
     3181 tggtctggca gactatgaca aagctgtcca ggaactggtc tttggcgccg gcagcgccgt
     3241 cattcaagag aaacgcgcca tcaccgtgca agccctgggc ggcactggcg cgctgaaatt
     3301 gggagctgat ttcatcaaac gtttctcacc agcatcgacg caagtctgga tcagcgatcc
     3361 aagctgggaa aaccaccgtg ccctgttcga gatggccggc ttcaccgtca acaactatcc
     3421 gtactacgat gcagctaccc gcggcgtcga tttcaccggc atgctggcag cgttgcagac
     3481 catgccggct ggctcggtcg tgctgctgca cgcctgctgc cacaacccga ccggcgccga
     3541 cctgaccgcc gatcaatgga cgcaggtcat cgacgtcatc acctcgcgca gtctgctgcc
     3601 tttcctcgac atggcttatc aaggttttgg cgacggtatc gaagaagacg gcagagtagt
     3661 gcgccgcttt gcagaggctg gcggtccgct gttcgtgtca aactcgttct cgaagtcctt
     3721 ctcgctgtat ggcgaacgcg tcggcgcatt gagcatcatc gccgccagca gcgacgaagc
     3781 agcgcgcgtg ctatcacaac tgaagcgcgt ggtgcgcact aactattcca acccgccgat
     3841 ccacggcggc cagatcgtcg caacggtatt agcagtgccg gaactgcgca tcctgtggga
     3901 aaaagaactg gccgagatgc gcgtgcgcat tcgtgaaatg cgtcaactgc tggtcaccag
     3961 gctgaaagaa aaggcatctt cacactgctt tgatttcgtc atcaaacaac gcggcatgtt
     4021 ttcgtactca ggcctgacca gggcgcaagt cgagcgcctg cgcaccgagt tctcggttta
     4081 cgctgtcgat accggccgta tctgtgtcgc tgcgttgaac accaagaata ttgatgccgt
     4141 cgccgacgcc atcgccaagg tgctgtaaaa attttttgac agaaaacaaa attccaatca
     4201 taattttggt cttatctttt ccctgatagc tcagtcggta gagcgacgga ctgttaatcc
     4261 gcaggtccct ggttcgagcc caggtcgggg agccaaagaa ttcaagggtt ccaagcgatt
     4321 ggaacccttt ttgtttgttc cgcaattttt gctaccagat aaaggtagcg gaaagtcacg
     4381 aaagcgaagg aaactctacg cacaatccca cgtgctcgcc gtcttcggtt caatgcttgt
     4441 cgttttgatt acatctcccc ccagatcgcg ttgatcgcag ccagcgcgca cagaccggca
     4501 gtttcggcac gcagtacgcg cggtcccatc gacagtatca gcgcgccatg gtcgcaggcc
     4561 agtttttctt ccgcttcgct gaagccgccc tcaggcccga tgagcaggct cagcgcttgt
     4621 gctgagtggt ggcgtgtcca gtccgatagc gactgctcgc cacgcggcga cagcaagatg
     4681 cgcctgtgcg gatcgctttg ggcgatccag tgcctgaagt cagagagcgg cgataactgc
     4741 gccagacggt tgcggccgct ttgttcagcg gcggcgcaga tgatgccttg ccagtggctt
     4801 tgtttctttt cggcgcgttc gctgttgaaa cgcaccacac agcgctgcgc ggccagcggc
     4861 tgtatgacag taacgcccag ttcgatggct ttttcgatga tccaatccat ttttgccgct
     4921 tccggcaagg cttgcgctag tgtaacggcg taggaaagct cggcttcgcg tggggaaaag
     4981 gtctggatct cggcatagac gcgcctcttt tccatccgcg taatgacggc ggcatattct
     5041 ccaccgcggc cattaaagag cgtcatggca ttaccgaccc gcatccggat gacctggatg
     5101 tgatgcgcca cctgatcagg caaggcgtgc gtcgtgccga tggcgaacgg gccgggacag
     5161 taaaagcgag gtaggttcaa gagactctga ttgggaaaga aaaaacaact gccttcaggc
     5221 ttgagtagcg aagtctgatt ttaattcttc aggctgctgt ctggtaaaat tcccggtcct
     5281 gtccggctag aaccgggggc cgacgaaaca gcaataacgg catgactggc atgcaatccg
     5341 caactctatg acatcgatca acatgatttc catgctccct accgacaaga tggccaatgc
     5401 gatccgcgcg ctggcaatgg acgcagtaca gaaagcaaat tcaggacacc caggcatgcc
     5461 aatggggatg gcggaaatcg cagtcgcctt gtgggccaag cactatcgtc ataacccggc
     5521 caatccgcaa tgggctaacc gcgatcgttt cgtgttatcg aacggacatg gttcgatgct
     5581 gcaatacgcg ttgctgcacc tgactggtta tgacctgtcg atggacgaca tcaggaattt
     5641 ccgtcagttg cactccaaaa ctgccggcca tccggagttt catattacgc caggcgtgga
     5701 aaccactacc ggcccgctcg gtcagggcat cgccaatgcc gtcggcatgg cgctggctga
     5761 aaagctgctc gccgctgaat tcaacaaacc cggcctggcc gtagtcgatc attacaccta
     5821 tgtctttgtc ggtgacggct gtctgatgga aggcatctcg cacgaagcct gctcgctggc
     5881 cggtgcctta cgcctatcca agctgatcgt tctgtacgac gataacggta tctcgattga
     5941 tggtcacgtc gaaggctggt tcag
//
LOCUS       DASRDH010000111         2461 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_485790,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000111 DASRDH010000000
VERSION     DASRDH010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485790"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            8..718
                     /gene="lolD"
                     /locus_tag="VFA14_02605"
     CDS             8..718
                     /gene="lolD"
                     /locus_tag="VFA14_02605"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008114663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HZS80307.1"
                     /translation="MNDVVTGSAIDPVLSGRGLSKTFAQGKYAVEVFSGIDIDIDTGE
                     QVAIVGASGSGKSTLLHLLGGLDTPTGGAVSLQGKNFAGLGEVARGNLRNAALGFVYQ
                     FHHLLPEFSALDNVAMPLMIRRVRRDQAQEQARAILARVGLENRVRHVPGELSGGERQ
                     RVALARALVTQPACVLADEPTGNLDRTTADKTFALMMELSQTLGTAFVIVTHDIELAR
                     RCGRVLRLAENGLSPYAA"
     gene            773..1945
                     /gene="argE"
                     /locus_tag="VFA14_02610"
     CDS             773..1945
                     /gene="argE"
                     /locus_tag="VFA14_02610"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006464015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008777 - acetylornithine deacetylase
                     activity [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HZS80308.1"
                     /translation="MNTRQWLEKLVAFDTTSRYSNLDLIATVRDSLQHQGITPWLVHN
                     EERTKANLFATLPATHGPGAGSTQGGIVLSGHTDVVPVDGQKWDSDPFMLTERDGLLY
                     GRGSCDMKGFIAAVLALVPEFLAMPRSKPLHLAFSYDEEVGCVGAPSMLAELQERGIR
                     PEGCVVGEPTNMKVVVAHKGINAFSCRVHGKSAHSSLTPQGCNAVEYAARLICTIRDF
                     ADAYKVDGPYDHLYDVPFTTMTTNQIRGGIAINTIPDLCEFTYEFRNLPGMSPDRMQA
                     EITDYVRDHLLPKMQAEYPDARIDIDSIASSPALETAEQEAITALVRALTEDQEKRKV
                     AYATEAGLFQRIGVPTIVCGPGEIAHAHKSNEFVAIAQLARCEEFLRKLGRSMQSA"
     gene            1962..>2461
                     /locus_tag="VFA14_02615"
     CDS             1962..>2461
                     /locus_tag="VFA14_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007879121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HZS80309.1"
                     /translation="MSSGCAHWIDTHCHLDAGEFVGEALAIADDAARQGVGMIVVPAA
                     SACQFQAVAALAAQRANCAYALGIHPLHVPAAQESDLLLLRRAVDSALEDERFVAIGE
                     IGLDFFVPALKDGVLRDRQEHFYREQLKLAREYDLPVLLHVRRSQDIILKYLRRIAVP
                     GGIAHA"
BASE COUNT          458 a          677 c          777 g          549 t
ORIGIN      
        1 ctgcgttatg aatgatgtgg tgactggctc agctatcgat cccgtgctgt ccggccgtgg
       61 tttgagcaaa accttcgcgc agggaaaata tgcggtcgag gtgtttagcg gtatcgatat
      121 cgatatcgat accggcgagc aggtcgccat cgttggcgct tcaggctccg gcaaatcgac
      181 gctgctgcat ttgctgggag gtctcgatac gccgaccggc ggtgccgtca gcctgcaagg
      241 caagaatttt gccggtctgg gggaagtggc gcgcggcaat ctgcgcaatg cggccttggg
      301 tttcgtctat cagtttcatc atttgctgcc ggagttctcc gcgctggaca atgttgccat
      361 gccgttgatg attcgtcgcg tcaggcgcga tcaggcgcag gaacaggcgc gcgccattct
      421 ggcgcgggtc ggtctggaaa accgtgtccg gcatgtaccg ggcgagttgt ctggcggcga
      481 gcgtcagcgt gtcgcactgg cgcgcgctct ggttacccag ccggcctgtg tgctggccga
      541 tgaaccgact ggcaatctgg accgcacgac cgccgacaag acttttgcgt tgatgatgga
      601 actgtcgcaa acgctgggta cggcctttgt catcgtcacg cacgacatcg agctggcgcg
      661 gcgctgcggc cgtgttttac gattggctga aaacggcttg tcgccgtatg ctgcctaatg
      721 aattgcatca tagtgaattg aacgtagttg actgaaacag ggagaatgca tcatgaatac
      781 acgccaatgg ctggaaaaac tggtcgcctt cgatacgacc agccgttatt ccaacctgga
      841 tctgattgcc actgtgcgtg acagtttgca gcatcagggc atcacgccct ggctggtgca
      901 taacgaggag cgcaccaagg ccaatctgtt tgcgacgctg ccggcaacgc acggtcccgg
      961 ggcgggcagc acccaggggg gcatcgtgtt gtccggtcat accgacgtgg tgccggtgga
     1021 cggccagaaa tgggacagcg atccatttat gttgaccgaa cgcgatggct tgctgtatgg
     1081 ccgcggcagt tgcgacatga agggtttcat cgccgccgtg ctggcattgg tgccggagtt
     1141 cttggcaatg ccgcgcagca agcctttgca tctggcgttt tcttatgatg aagaagtcgg
     1201 ttgtgtcggt gcgccgtcca tgctggccga attgcaggag cgtggtattc gcccggaagg
     1261 ctgcgtggtc ggagagccga ccaatatgaa ggtggtggtg gcgcacaagg gcatcaatgc
     1321 tttcagttgc cgcgtgcatg gcaaatcggc gcattcctcg ctcaccccgc agggctgcaa
     1381 tgccgttgaa tacgctgcgc gcctgatttg tacgattcgc gattttgccg atgcttacaa
     1441 ggtcgatggt ccttatgatc atctgtacga tgtgccgttt accaccatga cgaccaatca
     1501 gattcgcggc ggcattgcga tcaataccat tcctgatctg tgtgagttca cgtatgaatt
     1561 ccgcaatttg ccgggcatgt cgccggacag gatgcaggcg gaaatcaccg attatgtgcg
     1621 cgatcattta ttgccgaaaa tgcaggccga atatccggat gcccggatcg atatcgacag
     1681 catcgcctcc agcccggcgc tggaaacggc ggagcaggaa gccattaccg cgctggtgcg
     1741 cgcactgacc gaagaccagg aaaagcgcaa agtggcttat gccaccgagg ccggcctgtt
     1801 ccagcgcatc ggcgttccca ccatcgtgtg cggtcccggt gagatcgccc atgcgcacaa
     1861 gtcgaatgaa ttcgtagcga tcgcgcagtt ggcgcgctgc gaagaattcc tgcgcaaact
     1921 ggggcgctcc atgcagagcg cctgatcagg gtgaccctgt cgtgtcttct ggttgcgcgc
     1981 actggatcga tacgcactgc catctcgatg ccggcgaatt cgttggtgaa gcgcttgcca
     2041 tcgccgatga tgcggcaagg cagggtgttg gcatgatcgt cgtgccggcg gcgtcggcct
     2101 gtcagtttca ggcggttgcc gcgctcgctg cccagcgcgc caattgcgcc tatgcgctgg
     2161 gtatccatcc gctgcatgta cccgctgcgc aagaaagtga tctgctgctg ctgcgccggg
     2221 cggtcgattc ggcgttggag gacgagcgct ttgtcgctat tggcgaaatc ggcctcgact
     2281 tcttcgtgcc ggcgctgaaa gacggcgtct tgcgcgacag gcaggaacat ttttatcgtg
     2341 aacagctcaa gttggcgcgt gaatatgact tgccggtgct gctgcatgtc cggcgttcgc
     2401 aggacatcat tctcaaatac ctgcgccgca tcgctgtgcc cggcggcatt gcgcatgcct
     2461 t
//
LOCUS       DASRDH010000112         2777 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1286323,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000112 DASRDH010000000
VERSION     DASRDH010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2777
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1286323"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            116..1237
                     /locus_tag="VFA14_02620"
     CDS             116..1237
                     /locus_tag="VFA14_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS80310.1"
                     /translation="MVPEEILQGIQQRAVATLNASVVTDPVIRERVDYVCRCMSNRAG
                     VRLLMSCLLGKLDKPNVDLRKPYTEIGGTDSFSGRTYDERYLTKFISEHRLPLNPTTA
                     FLTPTLRNIDYALTTDRELVGRPRNLYKKTLELLEDVALQRIAADVLFVETVRVLMLL
                     RDEKLARMASLLDALERTEGALPLSSEAIATLVSQHLACKNASRLPVLVVAAAYESAG
                     TRLSESMLPLNSHNAADLQTNSLSDVEICLMGEDSVVTAYEMKMKRITQDDINVAVTK
                     IARAPNRIHNYLFVTTDVIDPMVAEYAATFYEKTDGTEIAILDCIGFLRYFLHLFHRI
                     RADYLNVYQALVLNEPDSAVSQTLKEAFLALRQAAESGE"
     gene            complement(1269..1649)
                     /locus_tag="VFA14_02625"
                     /pseudo
     CDS             complement(1269..1649)
                     /locus_tag="VFA14_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013393936.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
     gene            complement(1773..2087)
                     /locus_tag="VFA14_02630"
     CDS             complement(1773..2087)
                     /locus_tag="VFA14_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HZS80311.1"
                     /translation="MKRSLFIAALASGLPLQVFAQSTPADLARSKNCLACHSVNAKIV
                     GPAFKDVAQKYKNQKDVEDKLAQRVLKGSSGVWGAIPMPANAQVSEAEARMLVKWVLI
                     QK"
     gene            2251..>2777
                     /gene="lgt"
                     /locus_tag="VFA14_02635"
     CDS             2251..>2777
                     /gene="lgt"
                     /locus_tag="VFA14_02635"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HZS80312.1"
                     /translation="MLIHPMPDPIAFSIGPLQVHWYGLMYLLAFVQFLILGRMRIRQP
                     HIAAAGWKKEDLDDMLFYGALGVIIGGRLGEVLFYDPAYYFAHPADIIAVWKGGMSFH
                     GGFLGVLFAMYLWGRRHDRLLMDIMDFIAPLVPLGYAAGRLGNFINAELPGRIADPSL
                     PWAMIWPHVDSLPRHP"
BASE COUNT          598 a          771 c          779 g          629 t
ORIGIN      
        1 gaactacgat ccgaagaatc agaaagatct ctttgcgtga gggccggggt atcgtcaccc
       61 tgacgatctg catattttcc gtgtggtcgc agccacaaaa gcaaagggag tctcgatggt
      121 accagaagaa atactacagg gcatccagca gcgggccgtc gccacgttga atgcctctgt
      181 cgtcactgac ccggtaatcc gggagcgggt tgattatgtc tgccgctgca tgagtaaccg
      241 cgccggggta cggctgctga tgtcttgcct gttggggaag ctggacaaac cgaacgtcga
      301 tctgcgcaag ccttacaccg agatcggagg gacggacagc ttttccgggc gtacctatga
      361 cgaacgctac ctgaccaaat tcatcagcga gcaccggctg ccgctcaatc cgaccaccgc
      421 atttctgacc ccgacgctac gcaatatcga ctatgcgctg accaccgacc gagagcttgt
      481 cggcagaccg cgtaacctct acaaaaagac gcttgagcta ctggaagatg tagctttgca
      541 gcgcatcgcc gctgacgtgc tttttgtgga aaccgtgcgt gtgctgatgc tgctgcgcga
      601 cgagaagctg gctcgcatgg cttccttgct ggatgccttg gagcgtaccg agggcgcatt
      661 gcccctttcg tcggaggcca tcgccacgct ggtaagccag caccttgcct gcaagaatgc
      721 cagccgtttg ccggtgttgg ttgtcgctgc cgcttatgag tctgcaggaa cgcggttgtc
      781 cgaaagtatg ttgcctctga attcgcacaa tgcagcagat cttcaaacta actcattgag
      841 cgacgttgaa atttgtctta tgggtgaaga ttcagtcgtt actgcctatg aaatgaagat
      901 gaagcggatc acgcaggatg acatcaacgt ggcagtgacc aagatagcca gagcaccgaa
      961 caggatccac aactacctgt ttgtcaccac cgacgtgatc gacccgatgg tggccgagta
     1021 cgccgcgacg ttctacgaaa aaacggacgg caccgaaatt gccattctcg attgcatcgg
     1081 gttcttgcgg tacttcctgc acctgtttca ccgcatccgg gctgattatc tgaacgtcta
     1141 tcaggcgctg gttttgaatg agccggattc ggcagtcagc cagacactga aagaggcgtt
     1201 tctggcactg cggcaagccg ccgagagtgg cgagtgatcc agcatggaat caaaggcagt
     1261 catggtattc ataaagattg cgccttacag ccgctccacg ccaccagcag ccaggacgcc
     1321 agcaaggcga tgccgccgag cggcgtgatg gcaccgagga agcggatgcc ggtcagcgcc
     1381 agcatataca ggctgccgct gaaaatcacg atgccggtga gcatcgcact gccggcccag
     1441 ccgaggatga cggcatcaaa tcttggcatc agcagggcga cggcaaacat gcccaaggca
     1501 tgcgccagct agtaggtgac ggcggtttgc cagactgcaa gcatgtcggg atcgagcatc
     1561 cgtttcagtc catgcgcacc gaaggcgcca gcggcaacgg cgatgaacat gttcaaactt
     1621 gccagaatga cgagaagacg ttcgctcatg agggactcct acattcaaaa aagtcgatac
     1681 gatgtcgatc acattttgag ttaaatcctg tcggagcaat tgctcagcct aatgacgctc
     1741 tgatcgctga agacagccgg atcggcaagc agtcattttt gtatcagcac ccacttcacc
     1801 agcatacgcg cttccgcttc ggaaacctgc gcattggccg gcatcgggat ggcgccccat
     1861 acgcctgaac tgcctttcag cactctttgg gccagcttgt cttcgacatc tttctggttt
     1921 ttgtattttt gcgccacatc cttgaaggca gggccgacga ttttggcatt gaccgaatga
     1981 caggccagac aattctttga tctggccagg tcggcaggcg tcgattgcgc aaaaacctgg
     2041 aggggaagac ctgatgccag cgctgcgatg aataaagacc gtttcatgat tattttccag
     2101 gaagttttgc gtattctacg atctttgatc agccgctgct gtcggctggc atgagtatgg
     2161 cgcttgtgcc acaatccgtg tccgggctgt ggcgcctgcg gcatgatgcc atgccgctct
     2221 catccatcat cgtcttgaaa gaattcatcc atgctgattc acccgatgcc cgatccgatc
     2281 gccttttcca tcgggccgct gcaagtccat tggtatggtt tgatgtatct gctggcattc
     2341 gtgcagtttc tgatactggg ccgtatgcgc atcaggcagc cgcacatcgc cgccgccggc
     2401 tggaagaagg aagatctcga cgacatgctt ttttatggag cgctgggcgt aatcatcggt
     2461 ggccgtctcg gcgaagtgct gttttatgac ccggcatatt acttcgctca tccggccgat
     2521 atcatcgccg tatggaaagg cggcatgtcc ttccatggcg gcttcctcgg cgtgttattc
     2581 gcgatgtatc tgtggggccg cagacatgac cgtcttttga tggatatcat ggatttcatc
     2641 gcgccgctgg tgccgcttgg ttatgccgcc ggacgcctgg gcaatttcat caacgccgaa
     2701 ttgccgggcc gcattgccga tccgtcgctg ccgtgggcaa tgatctggcc gcatgtcgac
     2761 agtttgccgc gccatcc
//
LOCUS       DASRDH010000113        19459 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_733778,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000113 DASRDH010000000
VERSION     DASRDH010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 19459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19459
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_733778"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2990)
                     /locus_tag="VFA14_02640"
     CDS             complement(<1..2990)
                     /locus_tag="VFA14_02640"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HZS80313.1"
                     /translation="MNITALLQALKLAGITLEADGDNLKLYAPKGTLSAALRAQLVAH
                     KQAIIAWLGESATDDGEPLPVCTPDPGQRYAPFPLSDLQLGFYMADDPYMEFHVRPHY
                     YLEKNYTDLDVNRYQRAWNVALLRHRHEIVTVNADGMLEMVRDPVSLPVRCIDLRQED
                     PEAVRASLLQTRCEMMRSELPLQVWPWLDLRVSLWREDGVERARMHYNHNNFFSDGYG
                     TARLLQEVWQYYEAPDSELPVLHLSFRDAATALDALADSASGQAARRYWEDRLADLPP
                     PARLPVRTGLNRRCRSTLQRREGLVAADIWQGFKANAGLAGLTPSNAVFAVYAEILAA
                     WSNSPHFIVANMMTRRLNIHPEIRDILGNFASLYPLEIDLRDRASFTDSARRIQEQVI
                     RDARHLQWGGMKVMQALSRLKGNFGTAAVPFVVGSGLFMENFEHADFSCLETSQVMLD
                     HQFWELGDGRLYFVWDLLEEFFPDRLIDDMWAAYVGLLQRLADSPAAWLETHFSLVPK
                     HQLAQRAQSRVSSDDAPAGVLTELAAAAVRYAPQAVAVRCAQGDLSYAELTAANAAVA
                     AALNEAGMRQGQMVAIVADRGRDLVSAVHGTLLAGGVYVPIDPSLPEERRNYLLADSG
                     ARYVLAQRYYAGKLAWPENVRVLVIEDCRQHSGLAVAGFEQVQTGLAYLIYTSGSTGR
                     PKGVMIEHRGALNTIMDVNTRFGVNARDALFGVSSFGFDLSVYDLFGPVAAGASLVYP
                     ESRQALNPGHWLDRMIECDVTIWNSAPALAALLIEAAENRGVTLPHLRLVLMSGDWIP
                     TNLPARIKRIAPHARIVSLGGATEASIWSILFEIDEVDPAWSSIPYGYPMRNQSWFIL
                     DARLKPVPEWVAGDLYIGGVGLAQGYWQDAGKTAASFVTHPATGERIYRTGDLGRYLP
                     GGLIEFMGRSDAQVKIQGHRIELGEIESVLLAHPDIAAAVVLVQHSGASPQLAAHIVP
                     SRASLSADEIRRYLARAVPEYMVPR"
     gene            complement(2987..10360)
                     /locus_tag="VFA14_02645"
     CDS             complement(2987..10360)
                     /locus_tag="VFA14_02645"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZS80314.1"
                     /translation="MTSGSVETFEIETVSLRTVSEVIGAIAAARPMARALIFLENGEQ
                     ETGVCTYEELHCQAQKIACSLKNKYLAGERVLLMFHSGIDYVASFLGCLYAGVIAIPL
                     YPPRNNWHSARAAAIACDAGAKAILTMARLRADIATRMRAVNDQAVDGGMVEILAVDD
                     SDFLSSHAWVDPALRGSDLAYLQYTSGSTDTPRGVMVSHRNLLRNCAIGAQASDIGSG
                     ETFVSWLPIFHDMGLVQGMMMPLTLGGTVVFMPPAVFVQKPVRWLRAISRYRAGFSSG
                     PNFAYELCVSKIPQAEREGLDLSHWQVALNGSEPIALSTLRKFSKAFQPYGFSDAIFT
                     GGYGLAEATLYVTCGRRDEAPAVLYLDKAALTQNRAVVCEAGEPSSVPAVSSGRIQSE
                     PLVRIVSLDTGRICEAGEIGEIWVSGESVCQGYWQRPAESIDVFAAALADEPGVEFMR
                     TGDLGFTHDDELYVTGRIKDLIIIRGVNYYPQDIEATVENVHADLRKGGWGAAFTLRD
                     GVEERLVIVQEVERSARRCIDAAETGAAIARAVSLQHGIDSHTVVFVAPGGVPRTSSG
                     KIQRRACRDLFLAGELEELGRWERHSFSSIAAAGAEVLDPYAVQTKVRQIAAGLLYLA
                     PSALHDDTAFSELGLDSVRIVEFVSLVSEAFGVDLAPSVAFDHPTIAALSAHLTGLNA
                     GATGSSGTDEHIAIIGMDCRLPGANGTEEFWQLLATGKSAIKPVSAARRQLTGFGAGF
                     EGADDVSYRFGGFLDDIQHFDAGLFGIAPREAQSIDPQQRLLLQTAWHALETANIPPD
                     SLAGTATGVFVGVCASDYFNLQRDAGNLNAYSGTGNALSIAANRISYVFGLQGPSMAI
                     DTACSSSLVAVHQACRSLACGESTLALVGGVNLILNTDHSHIFSQANMLSPSGQCRTF
                     DDGADGYVRGEGCGVVVLKKLTDAVRDGDPVLAVIRGSAVNQDGHSNGLTAPNGLAQQ
                     RVITMALARAGVPAASIAYVETHGTGTPLGDPIEVQALQQVLGAGRQPDSLPCWLGAV
                     KTSIGHLEPASGIAGLIKTVLVLNRRAVPPNRNFATLNRHLRLDTSRLVMPTTLQEWP
                     ASADYPRRAGVSAFGFGGTNAHLVIEEYMAQPAPDERTPLCMPSFFLALSAKNGDSLA
                     QLAAAYAGRLAVLEHDPLALRAVCRAGVSGRAQLPERWAVVAATPAGLISALHDFPAG
                     PAASAGNCFCGRAAGTPRIAFLFTGQGSQYLGMGVELYQTEPVFREAIDRYDALLAPL
                     LRISIRDAMFEGMTAAPLEDTRYAQPALLVLQLALVALWAHWGIAPEFVIGHSIGEYA
                     AACVAGLLDAPAAVALVAERGRLMASAPGRGAMLSVDADWSAASVLLDGIALDLAAVN
                     APGQCVLSGDEQMISAAETMFIEQGIAVVRLQVSHGFHSRMMAPVLAGFAAEFGKAVL
                     GKGDRAMFIPTGGSVDADPGSAAYWIAQMRAPVRFNDALERALAAGADTFIEIGPRPT
                     LSRLGQRTHARGNWIAALREGRDAGNCMRAAAGALFVLGAPVNLRQLDRHLALPHHAA
                     PLYPFERTPFWFERRDLPAALPAAPATSDWPGRRVELAFDGVACFETSLPDAATAYLL
                     DHGVFGQSIMPAAGYLALARDAAKAIGLAQVPLMDIHFLRALPLADGEARLQTLLVRE
                     MDGRWTLQMASRCGTGDWQLHVRGVLNRLGTPLARIASVLPASFPSAGASAGIADFYA
                     LWAGRGLAYGPCFQAVQAVCSRHGVIHGRVSLPRGVPMPAGQILHPVLLDGAFQLVGH
                     ALQDMAAAATQIPVPAAVEEIITYASQAVAIWLVTVTLRAETGPTSMAADLAVYDSEG
                     QPVAHIRGLMLRWIDSVAIAQPARQLIPQCRRWQQIPSAADTAAPRCWRWLRLPLKSC
                     RQFTGLQEPSFPVIDSGQLMVSGDDTVVWEGMDAMLAELPASGGMIVQLPLLDSDHPV
                     ATSIAACRALQQLIVMLDRHGRLPDDFSLCVLTSGATSPLPGDTEISVAAAGATGLLK
                     SASLEYPHLRLRHLDLPVRPDGADRAALAHALALSDEISSAIRHGAVYAPRLQPLADS
                     AEASLPLLLDVAGAYLVTGGTGALGLHVAEWLAASGASHIVLASRQARPGPHGMARIE
                     ALRQRGVHIELRQADLEQHQAALELVRAIDRDERPLRGVVHAAGVSEDQALLRIDAAS
                     WQRVFDGKAQAALWLDEALASRKLDFFIGFSSVAAEFGSAGQSSYAAANALLDAVLLR
                     RHARTGDGCIVHWGPWAGEGMANDARLRQRWAQQGITPLNPATALAAMSRQLQTKTAS
                     AIIADIDWSAFSHHDQQGQGEGGVPAHAALCAAEVLMALDGPQAQGRIADGLAVLIGE
                     VLKMDAAREFADRARFCSTQLSSLGVDSLMGMELRNRVRAWVKVDLPAHVLIGGSTVQ
                     EVIEMIQQKLLVRSLSSASTSDDVQTDDDAEVLVL"
     gene            complement(10780..10855)
                     /locus_tag="VFA14_02650"
     tRNA            complement(10780..10855)
                     /locus_tag="VFA14_02650"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10820..10822),aa:Thr,seq:cgt)
     gene            complement(10956..11031)
                     /locus_tag="VFA14_02655"
     tRNA            complement(10956..11031)
                     /locus_tag="VFA14_02655"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10995..10997),aa:Glu,seq:ttc)
     gene            complement(11116..11191)
                     /locus_tag="VFA14_02660"
     tRNA            complement(11116..11191)
                     /locus_tag="VFA14_02660"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11156..11158),aa:Ala,seq:ggc)
     gene            complement(11261..12676)
                     /gene="gltX"
                     /locus_tag="VFA14_02665"
     CDS             complement(11261..12676)
                     /gene="gltX"
                     /locus_tag="VFA14_02665"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HZS80315.1"
                     /translation="MTSTVPGTPVRTRFAPSPTGYLHLGGARTALFSWAYARRFGGTF
                     ILRIEDTDLERSTPEAVRAINDGMQWLGLEHDEGPFYQMQRMDRYREMLAYMLQQGTA
                     YHCYCTPEEVEAMRERQRAAGEKPRYDGTWRPERGKTLPAIPEGRKPVVRFCNPLDGV
                     VTWNDAVKGPITIANSELDDLVIARPDGTPTYNFCVVVDDRDMQITHVIRGDDHINNT
                     PRQINILKALGGTLPQYGHVPMILGADGEKLSKRHGAVSVMDYAAQGYLPEAMLNYLA
                     RLGWSHGDEEIFSMPQFCGWFDLGHLSKSPAQFNPEKLAWLNNHYIKLADNERLATLT
                     RPLLEKEGVRFDNAPSLTQTIALLKGRVDTIKELAAAVRMFYRPPAPDAALVTQHITV
                     AIKPALRDFAARCVTVEWNRVALATMIKEVLAAHGWKMPLLAMPLRLIVVGQLQTPAI
                     DAVLALFSREVVLARLQHYLA"
     gene            complement(12786..13187)
                     /locus_tag="VFA14_02670"
     CDS             complement(12786..13187)
                     /locus_tag="VFA14_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4399 domain-containing protein"
                     /protein_id="HZS80316.1"
                     /translation="MAFRLVQTLFCATVLLASSAHAADPSVSFVAPMDGAAVSSPFDV
                     KFAVTGMEIRPAGDMTSNTGHHHLLINTGPITRDVLIPMDDTHLHYGKGQSDTRVTLS
                     PGKYQLTMQFANGAHLSYGPSMSKTIHITVK"
     gene            complement(13250..14338)
                     /gene="apbC"
                     /locus_tag="VFA14_02675"
     CDS             complement(13250..14338)
                     /gene="apbC"
                     /locus_tag="VFA14_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017454709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HZS80317.1"
                     /translation="MNITAEVVKAALSQVVDPNTGKDLVTGKSVRNIRLDGAGVTFDV
                     ELGYPANSQIALIRSAAEAAVRDVAGVGTVKANVYFKIVAHAVQSGIKLKSSIRNIIA
                     VASGKGGVGKSTTAVNLALALAAEGARVGILDADIYGPSQPMMLGIDARPETRDGKTM
                     EPLENHGLQVASIGFMIDPDAPMVWRGPIVTQALQQLLDQTNWRDLDYLIVDMPPGTG
                     DVQLTLSQKVPVTGAVIVTTPQDIALLDARKGLKMFEKVGIPILGIVENMSTHICSSC
                     GHAEAIFGSGGGEKMCADFGVDFLGALPLTMAIREQTDSGRPSVVAEPNGQVADIYRN
                     IARKLAVKVAAKARDMSSKFPSIVVQND"
     gene            14452..16497
                     /gene="metG"
                     /locus_tag="VFA14_02680"
     CDS             14452..16497
                     /gene="metG"
                     /locus_tag="VFA14_02680"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HZS80318.1"
                     /translation="MSTSSPRRLFVTTALPYANGAFHIGHIMEYIQADIWVRFQRMQG
                     HEVHFVGADDAHGAPIMIAAEKAGVTPQQFVAGIAAGRKQYLDGFHIAFDNWSSTDSQ
                     QNHALSQDIYRALRDDAGLITTRTIEQFFDPVKNMFLPDRYIKGECPRCGARDQYGDS
                     CENCSAVYAPTDLKNPCSTLSGATPVMKSSEHFFFRLSDPKCVAFLREWALNSNRLQP
                     EVINKAREWLDSAGGLGDWDISRDAPYFGIKIPDAPGKYFYVWLDAPIGYLASLKNYF
                     EKTGRDFDTFMADDRTEQYHFIGKDITYFHTLFWPAMLKFAGRKLPTNVFAHGHLTVN
                     GEKMSKSRGTGISPLRYLDIGMNPEWLRYYIAAKLNARVEDVDFNPDDFIVRVNADLI
                     GKYINIASRAAGFITRQFDGKLSMTWANDNDAFLANLRGIAPEIQALYAQREYGKALR
                     AVMEQADIINVYVDANKPWELARDTSRDAQLHEICSRLLEAFRILTVYLKPVLPQLAA
                     QVENFLNIAPQVWTDAQTALPDGHVINAYTHLMQRVDPKMLDALFDAPEPAADAGIEE
                     LAPEISIDDFAKIDLRIAKILHCEAVEGSTKLLRLTLDAGEGRTRNVFSGIASAYNPE
                     ELVGKLTVMVANLAPRKMKFGISEGMVLAASAKDEKAHPGIYVLSPWPGAEPGMRVR"
     gene            16511..18079
                     /gene="glpD"
                     /locus_tag="VFA14_02685"
     CDS             16511..18079
                     /gene="glpD"
                     /locus_tag="VFA14_02685"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HZS80319.1"
                     /translation="MADTDSLAARPEIYDLLIIGGGINGAGVARDAAGRGLKVALCEQ
                     NDFASATSSSSTKLIHGGLRYLEYYAFRLVAEALAEREHLLNIAPHIAWPLRFVMPHV
                     QGLRPRWMIRLGLWLYDHIGGHITLPHSRSITLDGMSSGLRANITRGFAYSDVWVDDA
                     RLVILNVLSAQEHGAAIFARTRLVNAGRSNGLWQCELEDTITGARRNLAAHTMVNTAG
                     PWVAQMDQLLNPGRQGKAHATVRLVRGSHIVVPRLFAGEHAYILQNDDKRITFMIPYE
                     DKFTLIGTTDVPQDNMDQGASISPEEEIYLLRAVNRYLIRMIGKADIVWRYAGVRPLF
                     DDDSGNPSAVTRDYTLALQDADNIPCVSVYGGKITTYRRLAESVLEKLAPWLKTRTHR
                     GPWSKEEALPGGNFKRADRAQELVDLQKEYARLNPHFLTRLFDRHGRNAHTILKEAQT
                     PAELGRDFGGGLVQREVDYLVTHEWACQSEDILWRRTKCGLHMSERQRADFADWFNAH
                     HIKHNDPAAPMTQP"
     gene            complement(18106..>19459)
                     /locus_tag="VFA14_02690"
     CDS             complement(18106..>19459)
                     /locus_tag="VFA14_02690"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZS80320.1"
                     /translation="ERGSVTLSVRQLSKQDRLSWVRFEIADTGIGISVDAQQRIFEYL
                     EQGDETISRRFGGTGLGLFLCRSLTHLMKGKISLSSNEGGSVFGVEIPFPLAEPLEQV
                     EDGLRPLQGSLIAVICEHPAWRRVLTTYVEKWGGSVVDPSMQAAPSISIIAGSTKGEP
                     QGATPLLGAVIVSSKGPVVPVMEANGRSRIEVTAFSMHALLEALLVLRGTSGDKPGST
                     PAEKTVAAPLNILVAEDNPVNMVLITHQLQALGYSAIRVARDGRDALEQWDQQEADVV
                     ITDMGMPHLDGSSLAREIRKRNPHAVIIATTAANPIDESDTWDVFTDVMRKPSSLDDV
                     RRVLADVRPAAHPAANAHAVNSSSGIDNNKLDQTLHSAFRESWASDRQKLHAAFMSQD
                     REKIFRTLHRIQGGLMTMGEDELAAECIRLQRDFGKVRHEEIARRYRNLLGRLDGLVS
                     "
BASE COUNT         4107 a         6078 c         5569 g         3705 t
ORIGIN      
        1 cgcggcacca tgtattccgg cacggcgcgg gcaagatacc tgcgtatttc atctgcgctg
       61 agcgatgccc ggctgggtac gatatgggca gccagctggg ggcttgcgcc gctatgctgc
      121 accagcacca cggcggcagc gatatcggga tgcgccagca gcacgctttc aatctcgcct
      181 aattcgatcc tgtgaccctg gattttgacc tgtgcatcac tgcggcccat gaattcgatg
      241 agaccaccgg gcaaataacg tcccaggtcg ccggtgcgat agatacgttc cccggtagcc
      301 gggtgtgtca cgaaactggc cgctgtcttc ccggcatctt gccaataacc ctgcgccaga
      361 ccgacgccgc cgatgtacaa atccccggcg acccattccg gcacaggctt gaggcgcgca
      421 tcaagaataa accaggattg attccgcatc ggatagccat agggaatgct cgaccatgcc
      481 ggatcgacct catcgatttc aaacagaatc gaccaaatcg acgcctcagt cgcgccgccc
      541 aggctcacta tcctggcatg cggcgcgatg cgtttgatgc gcgccgggag attggtcgga
      601 atccagtcgc cgctcatcag caccagacgc aaatgaggca gcgtcacccc ccggttttcc
      661 gcagcttcga tgagcagggc tgccaacgcc ggcgcggaat tccaaatagt gacatcgcat
      721 tcgatcatgc gatccagcca gtgccctgga ttgagcgcct gccgcgattc cgggtacacc
      781 agacttgcgc ccgccgcaac cgggccgaac aggtcataca cggacagatc gaaaccgaat
      841 gaggacacgc cgaacaaggc atccctggca ttgacgccaa agcgggtatt cacatccatg
      901 atggtattga gcgcgccgcg atgctcgatc atgacgccct tgggccgtcc ggtcgagccg
      961 gacgtgtaaa tcagataggc cagaccggtc tgtacttgct cgaatccggc aacggctaaa
     1021 cctgagtgtt gtcggcaatc ttcgatcaca agtacccgca cgttctccgg ccaggccagc
     1081 ttgccggcat agtagcgctg ggcaagcaca taccgggcac cagaatccgc gagcagatag
     1141 ttgcgtcttt cctcgggtaa cgaggggtcg atcggcacat atacgccgcc ggccagcagc
     1201 gtgccatgta ccgcactcac cagatcgcgg ccgcgatccg ccacgatggc caccatctgc
     1261 ccctgccgca tgcctgcttc gttcagcgcg gcggcaacgg cggcgttagc ggccgtcagt
     1321 tccgcgtagg acaaatctcc ttgggcgcag cgcaccgcca ccgcttgcgg agcataccgg
     1381 acggcagcag cagccagctc agtgagtacc cctgctggcg catcgtcact actgacccgc
     1441 gactgagcgc gctgcgccag ctgatgcttc ggaaccaggc tgaaatgtgt ttccagccat
     1501 gcggcggggc tatcggccag ccgttgcaac aggccgacat aagcggccca catgtcgtcg
     1561 atgagcctgt ccgggaaaaa ctcttccagc aaatcccata cgaaatacag cctgccgtcg
     1621 cccagctccc agaactggtg atccagcatc acctgtgagg tttcaaggca gctgaagtca
     1681 gcgtgctcga aattttccat gaaaaggccg ctgcccacta cgaacggcac ggcggcagtg
     1741 ccgaaatttc ccttgaggcg gctcagcgcc tgcatcacct tcatgcctcc ccactgtagg
     1801 tggcgggcat cacgaatgac ctgctcctgg atgcgccggg cgctgtcggt gaatgacgcc
     1861 cggtcgcgca ggtcgatttc cagcggatag agcgaagcga agttgcccag gatgtcgcga
     1921 atttcgggat ggatgttcaa gcgtcgcgtc atcatgttcg caacgatgaa atgagggcta
     1981 ttgctccatg ctgcgaggat ctcggcgtag acggcgaaca cagcgttcga tggcgtcagg
     2041 ccagccagtc cggcattcgc cttgaagcct tgccagatat ctgccgcgac cagaccttcg
     2101 cgacgttgca gcgtcgaacg gcagcgacgg ttcaagccag tgcggaccgg cagtcgggcc
     2161 ggtggcggca aatcggcaag gcgatcctcc cagtagcgcc gggcggcctg gccgctggcg
     2221 gaatctgcca aggcatcgag cgcggtcgcg gcatcgcgaa agctcagatg caacaccggc
     2281 aattcgctgt ccggcgcttc atagtattgc cagacctcct gcaacagtcg ggcagtgccg
     2341 tagccatcag aaaagaagtt gttgtggttg taatgcatgc gcgcgcgttc cacgccgtct
     2401 tcccgccata gcgaaacgcg cagatccaac cagggccaga cctggagcgg aagttcgcta
     2461 cgcatcatct cgcaacgggt ttgcagcaag ctggcacgaa cggcctccgg gtcttcctgg
     2521 cgcaagtcga tgcagcgcac cggcagagac accggatcgc gtaccatttc cagcatacca
     2581 tctgcgttaa cggtgacgat ttcatggcgg tgccgcagca gcgccacgtt ccaggcccgc
     2641 tggtaccggt tgacatccag atcggtgtaa tttttttcga gataataatg cggacggaca
     2701 tggaactcca tgtacgggtc gtccgccatg tagaaaccca gctgtaggtc cgataacgga
     2761 aacggcgcgt aacgctgccc cgggtcaggc gtgcagactg gtaacggctc gccgtcatca
     2821 gtggccgatt cgcccagcca tgcgatgatg gcctgcttgt gcgcgaccag ctgggcgcgc
     2881 aacgcggcgc tcagcgttcc cttaggcgca tacagcttca ggttatcgcc atccgcttcc
     2941 agcgtgatgc cggccagttt aagcgcctgc aacagtgcgg tgatgttcat agcaccagaa
     3001 cctccgcatc atcgtcggtt tgtacatcgt cgctagtcga cgcgctgctc aggctacgta
     3061 ccagcagttt ttgctggatc atttcgatca cttcctgcac cgtactgcca ccgatcaaga
     3121 cgtgcgccgg caagtccact ttgacccatg cccgcacacg gttgcgcagc tccattccca
     3181 tcagtgaatc gacgcccagt gacgacagtt gtgtggagca gaaccgcgcc cggtcggcga
     3241 actccctggc ggcgtccatc ttcaacacct caccgatcag caccgccaga ccgtccgcta
     3301 tccgtccttg cgcctgcgga ccatcaagcg ccatcaacac ttcagcagcg cagagtgcgg
     3361 catgtgcagg cacaccgccc tccccttggc cttgctggtc atggtgggaa aatgccgacc
     3421 aatcgatatc cgcaatgatg gcactggccg ttttcgtttg cagttgcctg gacattgccg
     3481 ccagcgcggt ggcgggattc aacggcgtta tcccttgctg cgcccatcgt tgacgcagtc
     3541 gcgcatcgtt ggccatgccc tcacctgccc acggccccca atggacgatg cagccatccc
     3601 cggtgcgggc atggcggcgc agcagtacag cgtcgagcaa ggcattggcg gcggcatagc
     3661 tgctctggcc ggccgagccg aattcggcag cgacggatga gaaaccgatg aaaaagtcca
     3721 gtttccggct ggcaagcgct tcgtccagcc acagtgcggc ttgggccttg ccatcaaata
     3781 cccgctgcca gctggcggcg tcgatgcgca acaaggcctg atcttccgag acgccagcag
     3841 catgcacgac accgcgcaag ggccgttcgt ccctgtcgat ggcgcgcacc agttccagcg
     3901 ctgcctggtg ttgctccaga tcagcctgcc gcagctcgat gtgaacgccg cgctggcgca
     3961 aggcttcgat ccgcgccatg ccgtgcggac cgggtcgggc ttgccgcgat gccagcacga
     4021 tgtggctggc gccggatgcg gccagccatt ccgcaacgtg cagcccaagc gcgccagtgc
     4081 ctccggtgac aaggtaagca cccgccacat caagcagcag cggcaaactc gcctctgcgg
     4141 aatctgccag aggctgcaga cgtggcgcgt agaccgcacc atgacggatg gcgctcgata
     4201 tttcgtcgct caacgccaag gcatgcgcca aggccgcccg gtcggcgcca tccggcctga
     4261 cgggcagatc gaggtgacgc aaccgcaggt gcggatattc cagagacgcc gatttcaaca
     4321 ggccagtcgc gccagctgcc gcaaccgata tttccgtgtc gcccggcaac ggactagtcg
     4381 cgccggacgt gagtacgcat aacgaaaaat cgtccggcag cctgccgtgc cggtccagca
     4441 tgacgataag ctgttgcagc gcccggcagg cggcaatcga cgttgcaacc gggtgatcgg
     4501 agtccagcaa cggcaattgc acgatcatac cgccgctggc cggcagttcg gccagcatgg
     4561 catccatgcc ttcccacacc acggtgtcat cgccgctcac catcagttgc ccgctatcga
     4621 tgacagggaa tgatggttcc tgcagcccgg tgaactgccg gcaggacttg agcggcaacc
     4681 gcagccagcg ccagcaacgc ggcgcggcag tatcggcagc gctggggatc tgctgccatc
     4741 gcctgcactg cggtatcagt tgccgcgcag gctgtgcgat agctacgcta tcgatccagc
     4801 gcagcatcag tccacggatg tgcgcgaccg gctggccttc actgtcgtag acagccagat
     4861 cggcagccat cgatgtcggc ccggtttcgg cgcgcagcgt gacagtcacc agccagattg
     4921 ccacggcctg gctggcgtag gtaatgattt cttcaaccgc ggctggcact ggtatttgcg
     4981 ttgctgccgc ggccatgtct tgcaaagcat ggccgaccag ctggaatgcg ccatcaagca
     5041 agaccggatg cagaatctgc cctgccggca tgggtacgcc tcgcggcagg gaaacacggc
     5101 cgtgaatgac gccatgtcta gagcaaaccg cctggaccgc ctgaaagcag gggccgtatg
     5161 caaggccgcg accggcccat agcgcataaa aatccgctat gccggccgaa gcgccggcag
     5221 atggaaaaga agcaggtaat acagacgcga tcctggccag cggcgtaccc agtctgttca
     5281 atacaccgcg cacgtgcagc tgccagtcgc ctgtgccgca gcgcgacgcc atctgtagcg
     5341 tccacctgcc atccatctcc ctgacaagca gggtctgcaa gcgcgcttca ccgtccgcga
     5401 gcggcaaagc ccgcaggaaa tggatgtcca tcagcggcac ctgcgccaaa ccgatggcct
     5461 tggctgcatc gcgagccaat gcgaggtaac cggcggcggg catgatgctt tgcccaaata
     5521 cgccgtgatc cagcagatag gcagtggcag cgtccggcaa ggatgtttcg aaacaggcga
     5581 cgccatcaaa cgccagttcc acgcgacggc ccggccagtc cgatgttgcc ggtgctgccg
     5641 gcagtgcggc tggaaggtcg cggcgctcga accagaacgg cgtgcgctcg aacggataga
     5701 gcggtgcggc atggtgtggc agcgccagat gccggtccag ctgacgcaaa tttaccggcg
     5761 cacctaaaac aaacaaggcg ccggcggcgg cccgcatgca gttgcctgcg tctctacctt
     5821 cgcgcagggc agcaatccag ttgccgcgcg catgcgtgcg ctggcccagt ctggacaggg
     5881 tcggcctcgg ccctatttcg ataaaggtat cggcgccggc tgccagggcg cgctcgagcg
     5941 catcgttgaa gcgcaccggc gcacgcatct gggcgatcca gtatgccgcg ctgcccgggt
     6001 cggcatcgac gctgccgccg gtgggaataa acatggcccg gtcgcctttg cccagaacgg
     6061 ccttgccgaa ctctgctgca aacccggcca gcacgggcgc catcatgcgg gaatggaaac
     6121 cgtggctgac ctgcaggcga acaacggcaa tgccctgctc gatgaacatg gtctcggcgg
     6181 cgctgatcat ttgctcatca ccggatagca cgcactgccc tggcgcgttg actgccgcaa
     6241 gatccagcgc gatgccgtcg agtaaaacgc tggcagcaga ccagtctgcg tctaccgaca
     6301 gcatcgcgcc ccggccaggc gccgacgcca tcaggcggcc acgctcggcc accagggcaa
     6361 cggcggcagg cgcatccagc aaaccagcca cacaagccgc ggcgtattcg cctatgctgt
     6421 gcccgatcac gaattcgggt gctatacccc agtgcgccca cagcgcgact agcgccagtt
     6481 gcagcaccag caaggccggc tgggcgtagc gggtgtcctc gagcggcgct gcggtcatgc
     6541 cttcgaacat ggcatcacga atcgatatgc gcagcagcgg cgcgagcagg gcgtcataac
     6601 gatcgatcgc ctcgcggaaa accggctcgg tttgatacag ctccaccccc atgccgaggt
     6661 actggctccc ttgccctgtg aacaggaaag caatgcgtgg cgtgccggcg gcccggccac
     6721 agaaacaatt gccagcggaa gccgccggcc cggcaggaaa gtcatgcaag gcgctgatca
     6781 atccggcagg tgtggctgca accaccgccc aacgttccgg caactgcgcc cggccactga
     6841 ccccggcccg acagaccgcg cgtagcgcga gcggatcgtg ctccagcacc gccagccggc
     6901 cagcgtaagc ggcggcgagt tgcgccaggc tgtcaccatt tttggccgac agggcgagga
     6961 aaaacgacgg catgcatagc ggcgtccgct catccggggc gggttgcgcc atgtattctt
     7021 cgatcaccag atgagcattg gtgccaccaa aaccgaacgc actgaccccg gctcggcgcg
     7081 gataatccgc cgatgcaggc cactcctgca atgtggtcgg catgaccaaa cggctggtat
     7141 cgagtcgcag atggcggttg agcgtggcga agttacgatt cggcggcaca gcccggcgat
     7201 tcagcaccag caccgttttg atcaggccgg caatgccaga ggcaggctcc aggtgaccga
     7261 tactggtttt gaccgcaccc agccagcacg gcaggctgtc tggctggcgg ccagcgccca
     7321 gcacctgctg tagcgcctgt acttcgatcg ggtcgcccaa gggcgtgcca gtgccgtggg
     7381 tttcgacata agcaatcgat gccgccggaa cacctgcccg cgccagcgcc atagtgatga
     7441 cccgctgttg ggccaggcca tttggcgcgg tcagaccatt gctgtgaccg tcctgattca
     7501 cggcggagcc acggatgact gccaataccg gatcgccatc gcgcacggca tcagtcagtt
     7561 ttttcagcac taccacacca cagccttcgc cgcgtacata accgtccgcg ccatcatcga
     7621 aggtccggca ttggccggac ggcgacagca tattggcctg gctaaagata tgactatggt
     7681 ccgtgttcag gatcaggttg accccgccaa ccagcgccag cgtgctctca ccacaagcaa
     7741 gactacggca ggcctgatgt acggccacca gcgacgacga gcaagcggtg tcgatcgcca
     7801 tgctcggccc ttgcaaaccg aagacgtagg aaatacggtt ggcggcaatg ctgagcgcgt
     7861 tacccgtgcc ggagtaggcg ttcaggttgc cggcatcgcg ttgcaggttg aaataatcgc
     7921 tggcgcaaac gccaacgaag acgccggtcg cagtgccggc cagactgtcg ggcggaatgt
     7981 tcgctgtttc cagcgcatgc caggcggttt gcagcagcag acgctgctgc gggtcgatgc
     8041 tctgcgcctc gcgcggcgca atgccaaaca gcccggcatc gaaatgctgt atatcgtcca
     8101 ggaagccacc aaagcggtac gacacatcat cagccccctc aaacccagcg ccaaagccgg
     8161 tgagttgccg gcgcgcggcg ctgaccggtt tgatcgcact ctttccggtt gccagcaatt
     8221 gccagaactc ctccgtcccg ttcgcgcccg gaaggcggca atccatgcca atgatggcga
     8281 tatgctcatc agtccctgac gacccggtgg cgccagcatt caagccggtg aggtgagcgc
     8341 ttagcgcggc aatggtggga tgatcgaagg ccacagatgg cgcgagatcg acaccaaatg
     8401 cctcagagac cagggagacg aattcgacga tgcgcaccga atccagaccc agttcgctga
     8461 aagcggtatc gtcgtgcaag gctgacggcg caagatagag aaggccggca gcgatctgcc
     8521 gcactttggt ttgtacggca tacggatcga gtacttccgc gccggcggct gcaatgctag
     8581 aaaaactgtg gcgctcccag cgccccagtt cttccaattc gccggccaga aacagatcgc
     8641 ggcaggcgcg gcgctgtatc ttgccgctgg atgttctggg cacgccgcct ggtgcgacga
     8701 agacaacggt atgcgaatcg atgccgtgct gcaggctcac ggcgcgggca atggctgcgc
     8761 ctgtctctgc cgcatcgatg cagcggcgag ccgagcgttc cacttcttgc acgatgacga
     8821 gacgctcttc tacaccgtcg cgcagcgtga atgccgcgcc ccagccgccc ttgcgcaggt
     8881 cggcgtggac gttttcgacc gtagcttcaa tgtcctgggg gtagtaattc acgccacgaa
     8941 tgatgatcag atccttgatg cgcccggtga catacaattc atcgtcatga gtgaagccca
     9001 gatcgccggt gcgcatgaat tcgacgcccg gttcatcggc cagcgcggcg gcgaatacgt
     9061 cgatgctctc ggctggccgc tgccagtaac cttggcacac actctcgcca ctgacccaga
     9121 tttcaccgat ttcaccagcc tcgcagatcc ggccagtgtc gagtgagacg atgcgcacga
     9181 gtggttcgct ctggatacgg ccgctggata cagccggcac ggatgatggc tcgccggcct
     9241 cacacaccac agcgcggttc tgcgtcagcg cggccttgtc caggtacaat acggccggcg
     9301 cttcatcacg ccggccgcag gtgacataca gggtggcttc ggccagccca tacccgccgg
     9361 taaaaatggc gtcgctgaat ccatacggct gaaaggcctt gctgaatttg cgcaaggtac
     9421 tcaaagcgat aggctccgaa ccgttcagcg cgacctgcca atggctcagg tcaagacctt
     9481 cccgctcggc ctgcgggatc ttcgagacgc acagttcgta ggcaaaattg gggccgctac
     9541 tgaagccggc ccggtaacgg ctgatggcac gcagccaacg cactggtttc tggacaaaga
     9601 ccgctggcgg catgaaaacg accgtaccgc ccaatgtgag cggcatcatc atgccctgca
     9661 ccaatcccat gtcatggaat atcggtagcc aggatacgaa ggtctcgccg gatccgatat
     9721 cggatgcctg cgcgccgatg gcgcagttac gcagcaggtt gcggtggctc accatgacgc
     9781 ctctgggcgt gtcggtcgaa ccggatgtgt attgcagata cgctagatca ctgccgcgca
     9841 gcgccggatc gacccatgca tgactggaca ggaagtccga gtcgtcgact gccagtattt
     9901 ccaccatgcc gccatccacc gcctgatcat tgactgcgcg catgcgggtt gcgatgtcgg
     9961 cgcgcagcct tgccatcgtc aatatcgctt ttgccccagc atcgcacgcg atcgcagccg
    10021 cgcgagcgga gtgccagtta ttgcgcggcg gatagagggg aatggcgatc acgcctgcat
    10081 agagacagcc aagaaacgag gcgacataat cgatccccga atggaacatc aacagaacac
    10141 gttcacctgc cagatactta ttttttagcg aacaggcgat tttttgggcc tgacaatgta
    10201 gttcttcgta agtacagacc ccggtttcct gctcgccatt ttccagaaaa atcaatgctc
    10261 ttgccattgg cctggccgct gcgatcgccc cgattacctc tgaaacagtt cgcagactga
    10321 ccgtttcgat ttcaaatgtc tctaccgatc cagaagtcat ctcttgacac acctccttga
    10381 tacgcacatt gaaaatcaat ctgatgcaat cgacgccgtc ccaccatctg catggtgcgc
    10441 cgccctatgc caccccattt caaggggaag atatacggat cgcatccagt gaaaaccaag
    10501 tggatggtaa tccctgagta tttttcagtc aattaaaaag cacgaaaatt tcccaagaaa
    10561 aaagcagcat tggaggtaga atttccaatt gaaattgagg aaattttatg aagaaatcgc
    10621 gtttttttga gaaccgaact tgtcagattt tttgtgtgcg ggaggtcatg agttgatact
    10681 gaaagccctt cccctgacta actcacgaaa attgaacgac aaaatccctc atagaaaacc
    10741 atgagggaag atgtgcaaga aacattgaat ccgatgagtt ggtgccgatg accgggatcg
    10801 aactggtgac ctacgcgtta cgagtgcgtt gctctaccaa ctgagctaca tcggcgttac
    10861 ctcgaatcct acctagcgca gccgtggatt atagactgaa caggagaaaa aacggaagcc
    10921 cctcatgcaa taaggtacac gtgtgctttg cgttctggcg tccccagggg gattcgaacc
    10981 cctgtactca ccgtgaaagg gtgatgtcct gggcctctag acgatgggga catatgtccc
    11041 gcagctgtta ctgcacgatg actgtgtggt atcaccattt gtcactcgcg ttttgcattt
    11101 tctactgccg ttcgctggtg gaggtaagcg ggatcgaacc gctgacctct tgcatgccat
    11161 gcaagcgctc tcccagctga gctatacccc cctgtgaaga agcgagatta tgcaacaggc
    11221 atcggtaaat agcaagcgcg ttttcccgga acctgtattt tcatgccaga tagtgttgaa
    11281 gtcgcgccag cacgacttcg cgactgaaca atgcgagtac ggcgtcgatc gccggcgtct
    11341 gcaattgacc gaccacgatc aggcgcagcg gcattgccag cagtggcatt ttccagccat
    11401 gcgcagccag cacttccttg atcatggtgg ccagcgccac cctgttccat tcgacggtaa
    11461 cgcagcgcgc ggcaaaatcc cgcagggccg gcttgatggc gacagtaatg tgttgcgtca
    11521 ccagcgcggc atccggcgca ggtgggcgat aaaacatccg caccgccgcc gccagttcct
    11581 tgatggtatc gacacgccct ttcagcaacg caatggtttg cgtcagggac ggcgcattgt
    11641 cgaaccgcac gccttctttt tccagcagcg gacgcgtcag cgtggcgaga cgttcattat
    11701 cggccagctt gatgtagtga ttattgagcc aggccagttt ttccggattg aactgcgccg
    11761 gtgatttcga caggtggccg agatcgaacc agccgcagaa ttgcggcatc gagaaaattt
    11821 cttcgtcgcc atggctccag cccagacgcg ccagatagtt gagcatggct tctggcagat
    11881 acccctgggc cgcataatcc atgacgctga ccgcgccgtg gcgcttggac agcttctcac
    11941 cgtcggcacc aaggatcatc ggtacatgac catattgcgg cagcgtgcct cccagcgcct
    12001 tgaggatgtt gatctggcgc ggggtgttgt tgatgtgatc gtcgccgcgg ataacgtgcg
    12061 tgatctgcat gtccctgtca tcaaccacga cgcagaagtt gtaggtcggc gtgccgtccg
    12121 ggcgcgcaat gaccaggtcg tccagctcac tgttggcaat cgtgatcggg cctttgaccg
    12181 cgtcgttcca ggtcacgaca ccatcgagcg gattgcagaa gcgcaccacc ggcttgcgac
    12241 cttccggaat cgccggcagc gtcttaccgc gttcgggacg ccaggtgccg tcatagcgcg
    12301 gcttttcgcc ggcagcgcgt tggcgctcgc gcatggcttc gacttcttcc ggtgtgcaat
    12361 agcagtgata agctgtcccc tgctgtaaca tgtacgccag catttcgcgg taacggtcca
    12421 tacgctgcat ctgataaaac ggaccttcgt catgctccag acccagccac tgcatgccgt
    12481 cgttgatagc ccgcaccgcc tccggcgtgg aacgttccaa gtcagtatcc tcgatacgca
    12541 gaatgaaggt gccgccgaaa cggcgcgcat aggcccacga gaacaaagcc gtgcgcgcgc
    12601 cgccgaggtg cagatagccg gtagggctag gcgcaaaacg ggtacgaaca ggggtgccgg
    12661 gtacagtgga tgtcatggta aataaaaacg gccttggtac accaaagccg tcatcggtct
    12721 gatcagaaag ccgtcatttt actgcgtcgg ccgccgccat ggagaaaaca ggcggaagac
    12781 gcgcttcatt taacggtgat atggatggtc ttgctcatcg acgggccata cgacagatgc
    12841 gcaccgttgg cgaactgcat ggtcaactgg tacttgccgg gcgacagcgt gaccctggta
    12901 tcgctctgtc ctttgccgta atgcagatgg gtatcatcca tcggaatgag tacatccctg
    12961 gtgatcggac cggtgttgat cagcagatga tggtgaccgg tattggacgt catgtcgccg
    13021 gccggcctga tctccatgcc ggtgacggca aatttgacgt cgaagggact actgacggct
    13081 gcgccgtcca tcggcgccac gaatgaaacc gaaggatcgg cggcgtgcgc ggacgaagcc
    13141 agcaagaccg tcgcacagaa tagtgtttgc accaaccgga atgccatggc atgcctcctt
    13201 gtaatgttga tcggatacat cgatgatgaa aagccgggca tgccagacat caatcgttct
    13261 gcaccacgat gctggggaat ttgctgctca tgtccctggc ttttgccgcc actttgaccg
    13321 caagcttgcg tgcaatgttc ctgtaaatat ccgccacctg gccatttggc tcggctacca
    13381 cggatggacg gccagaatcg gtctgttcac gaatcgccat cgtcagcggt aacgcgccaa
    13441 gaaaatcgac accgaaatca gcgcacatct tctcaccgcc accgctgccg aaaatcgctt
    13501 cggcgtgacc gcagctggaa caaatatgtg tgctcatgtt ttcgacgatg cccagaatcg
    13561 gaatgccgac cttctcgaac attttcaagc ccttgcgcgc gtcgagcaag gcgatgtcct
    13621 gcggcgtcgt cacgatcacg gcgccagtga ccggcacttt ttgcgacagt gtcaactgga
    13681 catcaccagt gcctggaggc atgtcgacga tcagataatc gaggtcacgc caattggtct
    13741 ggtcgagcag ttgctgcaag gcttgcgtga cgatcgggcc acgccacacc atcggcgcgt
    13801 ccggatcgat catgaagccg atcgacgcga cttgcaggcc gtggttttcc aatggttcca
    13861 tggtcttgcc gtcccgggtt tccggcctgg cgtcgatgcc cagcatcatc ggctgcgacg
    13921 gaccgtagat atcagcatcg agtatcccca cgcgcgcgcc ttcggccgcc agcgccagcg
    13981 ccagattgac tgcggtggtc gatttgccga caccaccctt gccggaggcg acagcgatga
    14041 tgttcctgat gctggatttg agcttgatgc cgctctgcac ggcatgcgca acgatcttga
    14101 aataaacatt ggccttgacc gtgccgacac ctgccacgtc ccgcacggca gcctcggcag
    14161 cgctacggat cagtgcgatc tggctattgg cgggatagcc cagctcgaca tcgaaggtga
    14221 cgccggcgcc gtcaagccgg atattcctga ccgacttgcc agtcaccaga tccttaccgg
    14281 tattggggtc gacgacctgg gaaagcgctg ctttgacgac ttctgcggtg atattcatta
    14341 cccctccttg atagataccc gcaagtctaa acccagtctg gcagatttgc tctgcgccac
    14401 aaccctcgcg gctgctagaa tggctctctt ttcctcacca ccgcattgcc aatgagtact
    14461 tcatcgccac gccgactctt cgttaccacc gcgcttccct atgccaatgg cgcattccac
    14521 atcggccata tcatggaata catccaggct gatatctggg tccggttcca gcgcatgcag
    14581 ggccatgaag tccatttcgt cggcgccgac gacgcccacg gcgcaccgat catgatcgcg
    14641 gctgaaaaag caggcgtcac gccgcaacag ttcgtcgccg gcatcgcagc aggccgcaag
    14701 cagtatctgg atggtttcca catcgccttc gacaactgga gttcgaccga cagccagcaa
    14761 aaccacgcgc tgtcacaaga tatctaccgc gctctgcgcg acgacgccgg tctgattacg
    14821 accaggacca tcgagcagtt cttcgacccg gtcaagaaca tgttcctgcc ggatcgctac
    14881 atcaaaggcg aatgccctag atgcggtgcg agggaccagt acggcgattc ctgcgaaaac
    14941 tgtagcgctg tgtatgcacc gaccgatctg aaaaatccct gctcgacctt gtccggcgca
    15001 acgccggtga tgaaatcgtc ggaacatttt ttcttcagac tgtccgatcc gaaatgcgtg
    15061 gctttcctgc gcgaatgggc gctcaacagc aaccgtctgc aaccggaagt catcaacaag
    15121 gccagggaat ggctggatag cgccggtggc ctgggggact gggacatcag ccgcgacgcg
    15181 ccctactttg gcattaaaat ccctgacgcg ccgggcaagt atttctacgt ctggctggat
    15241 gcgccgatcg gctatctggc gtcgctgaaa aattacttcg agaaaaccgg ccgtgacttc
    15301 gacaccttca tggccgacga caggaccgaa caataccatt tcatcggcaa agacatcact
    15361 tacttccaca cgctgttctg gcctgccatg ctcaagttcg ccggacgcaa gctgccgacc
    15421 aatgtcttcg cgcacggcca cctcaccgtc aatggcgaaa aaatgtccaa atcacgcggt
    15481 accggcatct caccgctgcg ttacctcgac attggcatga acccggaatg gctgcgctac
    15541 tatatcgccg ccaagctcaa cgccagggtc gaagacgtcg acttcaaccc ggatgacttc
    15601 atcgtccgcg tcaatgccga cttgatcggc aaatatatca acatcgccag ccgcgccgcc
    15661 ggtttcatca ccaggcagtt cgacggcaaa ctgagcatga catgggctaa tgacaacgac
    15721 gctttcctgg cgaatctgcg cggcatcgca ccagaaatcc aggcactcta cgcgcagcgc
    15781 gagtatggca aagccctgcg cgccgtgatg gaacaggccg atatcatcaa cgtttatgtc
    15841 gatgccaaca aaccgtggga gctggccagg gacaccagcc gtgacgcgca attgcatgaa
    15901 atttgcagcc gcctgctgga agctttccgt attctgaccg tgtacctgaa accggtactg
    15961 ccgcaactgg cagcacaggt cgaaaacttc ctcaatatcg cgccgcaggt ctggaccgac
    16021 gcacaaaccg cgctgcccga cgggcatgtc atcaacgcct acacccattt gatgcaacgc
    16081 gtcgatccga agatgctaga cgcgctattc gacgcgccgg aaccggccgc tgacgccggc
    16141 atcgaagaac tggcgccgga gatcagcatt gacgatttcg ccaagatcga tctgcgtatc
    16201 gccaaaatcc ttcattgcga agccgtagaa ggctcgacca agctgttacg cctgacgctg
    16261 gacgccggtg aaggcagaac ccgcaacgtg ttctccggca tcgcctcagc ctataatcca
    16321 gaagaactgg tcggcaagct gaccgtgatg gtagccaacc tggcgccacg caagatgaag
    16381 ttcggtattt cggaaggcat ggtgctggca gcgtcggcca aagatgaaaa agcccatccc
    16441 ggcatctatg ttttgagccc ctggccgggt gctgagccag gcatgcgcgt tcgctagcac
    16501 gctgtatgcc atggcagaca ccgactcttt ggcagcccgc ccggaaatct atgatctgct
    16561 gatcatcggc ggcggcatca acggcgccgg cgtagcgcgt gacgccgccg gacgcggact
    16621 caaggtagcg ctgtgcgaac agaacgattt tgccagcgcc acttcctcgt ccagcaccaa
    16681 gctgatccat ggcggcctgc gctatctcga atattacgca ttccgtctcg tcgccgaagc
    16741 gctcgccgaa cgtgaacatt tgctcaacat cgcgccgcac atcgcctggc cgctgcgctt
    16801 cgtcatgccg cacgtccagg gactgcgtcc acgctggatg atccgcctcg gtttgtggct
    16861 gtacgatcat atcggcggcc atatcacgct gccgcattcg cgcagcatca cactggacgg
    16921 catgagcagc ggtctgcgcg ccaacatcac gcgcggcttc gcctactctg acgtctgggt
    16981 tgacgacgcg cggctggtga tcctgaatgt gctaagcgca caggaacacg gcgccgctat
    17041 cttcgcgcgt acccgcctcg tcaatgccgg acgcagcaat ggcctgtggc aatgcgaact
    17101 ggaagatacc atcaccggcg cacgccgcaa cctcgcagca catacgatgg tcaacaccgc
    17161 cggcccctgg gtggcgcaga tggaccaact gctcaatccg ggccggcaag gcaaggcaca
    17221 cgccaccgtc agactggtgc gcggcagcca tatcgtcgtg ccgcgtctgt ttgccggcga
    17281 acacgcttac atcctgcaaa acgatgacaa gcgcatcacc ttcatgattc cttacgagga
    17341 caaatttacg ctgatcggca ccaccgacgt cccgcaagat aacatggacc agggcgccag
    17401 catcagtccg gaagaagaaa tctacctgct gcgcgccgtc aaccggtact tgatcagaat
    17461 gattggcaaa gccgacatcg tctggcgcta cgccggcgtg cgtccgctgt tcgacgatga
    17521 ctccggcaat ccatcggcag tcacacgcga ctacacactg gcgctgcagg atgcagacaa
    17581 tattccctgc gtatctgtct acggcggcaa gatcaccacc tatcgtcgcc tggctgaatc
    17641 cgtactggaa aaactggcgc cgtggctcaa gaccaggaca caccgcggtc cgtggagcaa
    17701 ggaagaggct ttacccggcg gcaacttcaa gcgcgccgac cgggcgcagg aactggtcga
    17761 tttacaaaag gaatacgccc ggctcaaccc tcatttcctg acgcgcctgt tcgaccgcca
    17821 tggccgcaac gcccatacca tcctgaaaga agcacaaacg ccagctgaac tgggccgcga
    17881 tttcggtggt ggactggtgc agcgcgaagt cgattatctg gtcacgcacg agtgggcttg
    17941 ccagtccgaa gatatcctgt ggcgccgtac caaatgcggc ttgcacatga gcgaacggca
    18001 acgtgccgac tttgccgact ggttcaatgc ccatcacatc aagcataacg atccggccgc
    18061 gcccatgaca cagccttgac ccgctttccc gcgccaagaa cgtgtctacg ataccagccc
    18121 atcgaggcgg cccaacaggt tccggtatcg ccgagctatc tcttcgtggc gaactttgcc
    18181 gaaatccctt tgtaaacgta tacattccgc cgcaagttcg tcttctccca ttgtcatcaa
    18241 cccaccctgg atacggtgga gagtacgaaa gattttttcc ctgtcctgcg acatgaaagc
    18301 cgcatgcaat ttctgtctgt cgctcgccca gctttcgcga aaagcactgt gcagcgtctg
    18361 atccagtttg ttgttatcta tgccggatga ggaattgaca gcatgagcat tggcagccgg
    18421 atgagcggcg ggccgcacgt cggcgagtac tctgcgcacg tcgtccagtg aactgggctt
    18481 gcgcatgaca tcagtgaata catcccatgt atctgattcg tcaatcgggt tggctgcggt
    18541 ggtcgcgatg atgacagcat gcgggttccg cttgcgaatc tctctggcca gactggaacc
    18601 atccagatgc ggcatgccca tgtcggtaat gacgacgtct gcttcctgct gatcccattg
    18661 ctcgagcgcg tcccgaccgt ctctggcgac gcggatcgcc gaatagccga gtgcctgcag
    18721 ttgatgtgtg atcaggacca tattcaccgg attgtcttca gccaccagaa tattgagcgg
    18781 tgctgctacg gttttttctg ccggcgtgct gcctggcttg tcccccgatg tacctctcaa
    18841 gacaagtaat gcttccagca acgcatgcat ggagaaggca gtgacctcaa tgcggctacg
    18901 gccattggcc tccatcacag ggaccacggg tcctttcgat gaaacgatga ctgctccaag
    18961 gagaggcgtc gccccttgcg gctccccctt cgtgctcccg gcaataatgg aaatagaggg
    19021 cgccgcttgc atagaaggat cgacgacact gccgccccac ttttcgacat atgtcgtcaa
    19081 aacccgtcgc catgcaggat gttcgcagat gaccgcgatc aatgatcctt gcagtggccg
    19141 tagaccatcc tcaacctgtt caagcggctc cgccagcgga aatggaatct caacaccaaa
    19201 gacgctcccg ccttcgttac tggacaagct tatttttcct ttcatcaagt gggtgagact
    19261 gcggcaaaga aacaagccca gaccagtccc gccgaatcgc ctgctgatgg tttcatcccc
    19321 ctgctccaga tattcaaaaa tccgctgttg cgcatccacc gagatgccga tgccggtatc
    19381 ggcgatttca aaacgcaccc atgacaaacg gtcctgtttg ctcaactggc gcaccgataa
    19441 agtcacagag ccgcgctcg
//
LOCUS       DASRDH010000114         2334 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1283313,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000114 DASRDH010000000
VERSION     DASRDH010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1283313"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..829)
                     /locus_tag="VFA14_02695"
     CDS             complement(<1..829)
                     /locus_tag="VFA14_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZS80321.1"
                     /translation="MDNFKIAAIDAGKTTPIEADAVIIGAGPVGLFQVFELGLLEIRA
                     HVIDSLANVGGQCTELYPDKPIYDIPAVPVCTGQELTDNLLKQIEPFNPTFHLGQEVT
                     LVHKREDGRFDLKTSAGTRFISKTIFIAAGVGSFQPRTLKVDGIEAFEGSQLFYRIKE
                     PALFNSKNIVICGGGDSALDWTLNLIGKAESVTLLHRRAEFRAAPASVAKMKERCEQY
                     EMQFLVGQATGFEARDGKLSEIKVTGADGVTRRLPLDMLLIFFGLSPKLGPIAEWGLD
                     "
     gene            complement(988..1311)
                     /gene="fdxA"
                     /locus_tag="VFA14_02700"
     CDS             complement(988..1311)
                     /gene="fdxA"
                     /locus_tag="VFA14_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013233640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin FdxA"
                     /protein_id="HZS80322.1"
                     /translation="MTHVVTESCIRCRYTDCVDVCPVDCFREGPNFLAIDPDECIDCA
                     VCVAECPVNAIYAEEDVPSDQQEFIRLNEQLARSWPSITKTKAPLADADEWKDVRNKL
                     EFLQR"
     gene            1657..>2334
                     /locus_tag="VFA14_02705"
     CDS             1657..>2334
                     /locus_tag="VFA14_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008327420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HZS80323.1"
                     /translation="MIISSLLDTDLYKFTMMQVVLHHFPCAQVEYRFKCRNEDVDLTP
                     YIDEIRMEIAALCRLRFQEQELDYLRSFRFIKSDFVDFLGLFHLSEKYISVAPSPAGN
                     GEIDIQVRGPWLHTILFEVPVLAIVNEVYFRATQRTPDYATGRARLLDKIALLTDNPL
                     LEGCKIADYGTRRRFSRSWHAEVILTMKERMGSHFAGTSNVQFAMQHDLTPLGTMAHE
                     YLQACQAL"
BASE COUNT          496 a          633 c          670 g          535 t
ORIGIN      
        1 tatccaggcc ccattcggcg atcgggccga gtttgggcga caggccgaag aatatcagta
       61 gcatatccag cggcaggcgg cgggtaacgc catcggcgcc ggtgaccttg atctcggaca
      121 gtttgccgtc cctggcttcg aagccggtcg cctgaccgac caggaattgc atttcatact
      181 gctcgcatcg ctccttcatt ttggctaccg atgccggcgc cgcgcggaac tccgcgcgac
      241 ggtgcagcag tgtgaccgat tccgctttac cgatgaggtt cagcgtccag tccagcgcag
      301 agtcaccgcc gccgcagatg acgatattct tgctgttgaa cagggcgggt tccttgatgc
      361 ggtagaacag ttgcgaacct tcgaaggctt cgatgccgtc gaccttcagc gtgcgcggct
      421 ggaacgagcc gacgccggca gcgatgaaga tggtcttgct gatgaatctg gtgccggccg
      481 aggttttcag gtcgaagcgg ccatcttcgc gcttgtggac gagggtgact tcctgaccca
      541 gatggaaggt gggattgaac ggttcgattt gtttgagcag attgtcggtc agttcctggc
      601 cagtgcagac tggtacggca gggatgtcgt agatcggctt gtccggatac agttcggtgc
      661 attggccgcc gacgttggcg agcgagtcga tgacgtgcgc cctgatttcc aacaggccca
      721 gttcgaatac ctggaacagg ccgacagggc cggcgccgat gatgacggca tccgcttcta
      781 tcggtgtcgt tttgccagca tcgatggctg caatcttgaa attatccata aaaaatccag
      841 ttcccgtttc agttgaggag ggcggacgca attggcgatc ggtcaaggcg aatcggcgcg
      901 ggcgctcccg gtcttggtag tgtggctatg tgaaacttgt gggttgcgat acgaaacgtt
      961 gacgccgacg ccgcgcaggc gcagtattta gcgttgcagg aattccagct tgttcctgac
     1021 gtctttccat tcgtcagcat ctgccagagg ggctttggtt ttggtgatgg agggccagct
     1081 acgtgccagc tgctcattga gcctgataaa ttcctgctgg tcggacggga cgtcttcttc
     1141 cgcatagatt gcattgaccg gacattccgc aacgcacacg gcgcagtcga tgcattcatc
     1201 cgggtcgatg gcgagaaaat tcggaccttc gcggaagcaa tcaaccggac atacatcgac
     1261 gcagtccgta tagcggcagc ggatgcagga ttcggtcaca acatgggtca taccagtcct
     1321 atctgtaagt ttctttgaaa ccgatgtcaa atccagccgg aacccaagca gccggaaccc
     1381 aagcaatact aagccgctgc ctgggtaaca gtctccataa aatcaaccga ctgaacaacc
     1441 gcacatttta aggcaattca atttttccca caaatgaggc ttatacagat tacgtataag
     1501 taaatacgaa gtagcgtgag gtatgcattc gtccggaggc cgcggtgaaa tgaattttcg
     1561 ccatgcgtac agatgcggtg cggtaagtta agatagtatc cgtttgcatt tcccccagca
     1621 ttttcgcagg tgatgctgcg cgcgtcgcca gaacatatga tcatttcttc tttgctcgat
     1681 accgatttgt ataaattcac catgatgcag gtggtgctgc atcatttccc gtgcgcccag
     1741 gtcgaatacc gtttcaaatg ccgcaatgaa gatgtcgatc tgacaccgta catcgacgaa
     1801 atcaggatgg aaatcgccgc gctgtgccgc ttgcgtttcc aggagcagga actggattat
     1861 ctgcgcagtt tccgcttcat caaaagcgac ttcgtcgatt ttctcggcct gtttcacctc
     1921 agcgaaaaat acatcagcgt cgcgccgtcg ccggccggta acggcgagat cgacatccag
     1981 gtcagaggac cgtggctgca caccattctg ttcgaagtgc cggtactggc tatcgtcaat
     2041 gaggtatatt tccgcgcgac ccagcgtact cccgattatg caaccggccg cgcgcgcctg
     2101 ctcgacaaga ttgcgctgct gaccgacaat cccttgctgg aaggctgcaa gattgccgat
     2161 tatggcacac gtcgccgttt ctcgcgcagt tggcatgcgg aggtgatcct gaccatgaag
     2221 gagcgtatgg gcagccattt cgccggcacc tccaatgtgc agttcgcgat gcagcacgac
     2281 ctgacgccgc tgggtacgat ggcgcacgag tatctgcaag cctgccaggc gctc
//
LOCUS       DASRDH010000115         2979 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_531914,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000115 DASRDH010000000
VERSION     DASRDH010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2979
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_531914"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2280
                     /gene="pepN"
                     /locus_tag="VFA14_02710"
     CDS             <1..2280
                     /gene="pepN"
                     /locus_tag="VFA14_02710"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HZS80324.1"
                     /translation="RPDVMGKYTVMLRADRKTCPVLLSNGNLIEAGDLGDGRHYAKWE
                     DPFRKPSYLFALVAGNLVRREEKYRLQSGREVLLQVWVEEGNLDKTRHAMDSLKNSIR
                     WDEQRFGLELDLDRFMIVAVSDFNMGAMENKGLNIFNTRFVLANPRIATDADYAGIEA
                     VVGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSADMIGSASGRVVKRIDDVRV
                     LRQMQFPEDAGPMAHPVRPDSFVEINNFYTVTVYEKGAEVVRMYQTLLGHDGFRRGMD
                     LYFKRHDGQAVTCDDFRAAMADANKRDLKQFERWYSQAGTPRVTVSTHYDASAKTLAL
                     TLAQSCPVTPGQKKKLPFHIPVAIGLLDCKGQDMALTLSGEKPGKQAQTTRVLELTQT
                     RQTFIFTDVAEKPVPSMLRGFSAPVALEYVYSDAELAFLMAHDSDPFNRWEAGQRLAM
                     RRLLHLTAAVQAANQSCMPLASVLGRLQPDDALTDALRATLKDASLDPAFRELVLTLP
                     SETMIAEQMEVIDPQAVHTARQFLRCMLAQELRADLLAAYEANRNSGAYSPDAAASGR
                     RALKNVALSYLIELDDAPVHALAQQQYNDANNMTDRLAALTALSNSRAEGKAMALEAF
                     YTEFEQEALVIDKWFTLQATARTADVGTVRALARHPAFTLKNPNRARSLLFSFCNGNP
                     SAFHALDGSGYAFWAEQVITLDSSNPQVAARLARSLDRWRRYAPALQEKMVAALRQVA
                     GVPNLSRDVAEVVTKSLTN"
     gene            2320..>2979
                     /locus_tag="VFA14_02715"
     CDS             2320..>2979
                     /locus_tag="VFA14_02715"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HZS80325.1"
                     /translation="MTKRISLTQHLIEQQRLYNRIPSELRLLIEVVGRACKSISHAVG
                     KGALGDVLGSVGSENVQGEVQKKLDIISNEILLEANEWGGHLAGMASEEMETIHLIPN
                     RYPKGEYLLMFDPLDGSSNIDVNVSIGTIFSVLKAPEGMITPSEQDFLQAGVRQVAAG
                     YAIYGPQTVLVLTTGNGVHCFTLDLEMGAWVLTQRDMQIPVDTREFAINASNARHWYP
                     PV"
BASE COUNT          629 a          860 c          875 g          615 t
ORIGIN      
        1 cgtcctgacg tcatgggcaa atataccgtc atgctgcgcg ccgacaggaa gacctgtccg
       61 gtgctgctgt ccaacggcaa tctgattgaa gcaggcgacc tgggcgatgg ccgtcactac
      121 gccaagtggg aagacccgtt caggaagcct tcctacctgt ttgcgctggt ggccggcaat
      181 ctggtccgcc gggaagaaaa gtaccggctc cagtccggcc gtgaagtgtt gctgcaagta
      241 tgggtggagg agggtaacct tgataaaacc cggcatgcca tggactcgct caagaatagt
      301 attcgttggg atgaacagcg cttcgggctg gaactcgatc tcgatcgttt catgatcgtt
      361 gccgtcagtg acttcaacat gggcgcgatg gaaaacaaag gtctcaatat tttcaacacc
      421 aggtttgtgt tggctaatcc acgcattgcc accgatgctg actacgccgg catcgaggcc
      481 gtggtcggtc acgaatattt ccacaactgg accggcaatc gcgtcacctg tcgtgactgg
      541 ttccagttgt cgctgaagga aggactgaca gtctttcgcg atcaggagtt ttccgccgac
      601 atgatcggca gtgccagcgg tcgcgtggtc aagcgtatcg acgacgtgcg tgtgctgcgt
      661 cagatgcagt ttccggaaga cgccggcccg atggcgcatc cggtgcgtcc tgattctttc
      721 gtcgagatca ataatttcta tactgtcact gtctacgaaa agggcgccga agtggtgcgt
      781 atgtaccaga ctctgcttgg tcatgacggt tttcgcaggg gtatggatct gtatttcaaa
      841 cgtcatgacg gccaggccgt cacctgcgat gattttcgtg ccgccatggc agacgccaac
      901 aagcgcgacc tcaagcagtt cgagcgctgg tatagccagg ccggcacgcc gcgtgtgacg
      961 gtatcgacgc actatgacgc cagcgcgaag acgctggcgc tgactctggc gcaatcctgt
     1021 cccgttacgc cgggacaaaa gaaaaagctg ccgttccata tccctgtcgc catcgggctg
     1081 ctcgactgca aggggcaaga catggcgctg accctgtccg gcgaaaagcc cggcaagcag
     1141 gcgcagacca cgcgtgtgct ggaattgacg caaaccagac agactttcat ctttaccgat
     1201 gtcgccgaaa agccggtgcc gtccatgttg cgtggtttct ccgcgccagt ggcgctggag
     1261 tacgtctata gcgacgccga actggctttc ctgatggcgc atgatagcga ccccttcaat
     1321 cgttgggaag ccggccagcg tcttgccatg cgccgtctgc tccatctgac tgcggccgtg
     1381 caggccgcga accagtcctg catgccgctt gcatcggtac tcggcaggct gcaaccggac
     1441 gatgcactga ccgatgcttt gcgcgctacg ctcaaggatg cgtcgctgga tccggcgttc
     1501 cgtgaactgg tattgaccct gccgtcggaa accatgatcg ctgaacagat ggaagtcatc
     1561 gatccgcagg ccgtccatac cgcgcgccag ttcctgcgct gcatgctggc gcaggaactg
     1621 cgcgccgatc tgctggctgc ctatgaggcc aaccgaaaca gcggtgccta tagtcctgat
     1681 gctgccgcct cgggcaggcg cgcgctgaag aacgtcgcgc tatcttatct gatcgaactc
     1741 gacgatgcgc cggtgcatgc gctggcgcaa cagcaataca atgacgccaa caacatgact
     1801 gatcgcctcg cggcgctgac cgcattgagc aacagccgcg ccgaaggcaa agccatggca
     1861 ttggaagctt tctacaccga attcgagcag gaagccctgg tcatcgataa atggttcacg
     1921 ctgcaggcca ctgcgcgcac tgccgatgtc gggactgtgc gcgcattggc tcgccatccg
     1981 gcctttacgc tgaagaatcc gaatcgcgcg cgcagcctgc tgtttagttt ctgcaacggt
     2041 aatccgtcgg cttttcacgc gctggacggc agcggttatg cgttctgggc agaacaagtc
     2101 atcacgctcg acagcagcaa cccgcaagtc gcggcccgtc tggcgcgtag cctggatcgc
     2161 tggcgtaggt acgcgccagc attgcaggaa aaaatggtcg ccgcactgcg tcaggtcgcc
     2221 ggtgtgccga atctgtcgcg cgacgtcgcc gaggtcgtca ccaagtctct gacgaactga
     2281 acacggcaag acaggattgg attttttaga aagagcatca tgactaaacg catcagcctg
     2341 acccagcacc tgatcgaaca gcaacgtctg tataacagaa ttccttccga attgcgtctg
     2401 ctgatcgaag tcgttggccg cgcctgcaaa agtatcagcc acgccgtcgg caagggcgcg
     2461 ctgggggacg tgttgggcag cgtcggcagt gagaatgtgc agggtgaagt acagaaaaaa
     2521 ctggacatca tctccaacga aattctgctc gaagccaatg aatggggcgg ccacctggcc
     2581 ggtatggcat cggaagaaat ggagaccatc cacctgattc ccaaccgtta tcccaagggt
     2641 gaatacctgt tgatgttcga cccgctggac ggttccagca acatcgatgt caacgtttcc
     2701 atcggcacca tcttttctgt gctgaaggcg cccgaaggca tgatcacgcc atcagaacag
     2761 gactttctgc aagcaggggt caggcaggtc gctgccggtt atgccatcta cggtccgcaa
     2821 actgtgttgg tgctgaccac tggcaatggc gtgcattgct ttacactgga tctcgagatg
     2881 ggtgcctggg tgctgaccca gcgcgacatg cagattccgg tcgacaccag ggagtttgcg
     2941 atcaacgcct ccaatgcgcg tcactggtac ccgccggtc
//
LOCUS       DASRDH010000116         2672 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_361124,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000116 DASRDH010000000
VERSION     DASRDH010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361124"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="VFA14_02720"
     CDS             <1..259
                     /locus_tag="VFA14_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS80326.1"
                     /translation="LLRVEAGRALVAPNKMAQGLATYKAATPWRTRAERGVRAGHGQI
                     ACSSPSGKWRTRSPVRYLRTPAWPANGLCTKRPAINSDQFM"
     gene            complement(464..976)
                     /locus_tag="VFA14_02725"
     CDS             complement(464..976)
                     /locus_tag="VFA14_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HZS80327.1"
                     /translation="MLPDSFHRDLPDSFHRDGYMVTRLDDLANWARTGSMWFMTFGLA
                     CCAVEMMHAASARYDFDRFGVMPRASPRQSDVMIVAGTLTNKMAPALRKVYDQMAEPR
                     FVISMGSCANGGGYYHYAYSVVRGCDRIVPVDVYVPGCPPTAEALLYGIVQLLNKVRR
                     GATSANTVAR"
     gene            1282..1572
                     /gene="groES"
                     /locus_tag="VFA14_02730"
     CDS             1282..1572
                     /gene="groES"
                     /locus_tag="VFA14_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013436121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HZS80328.1"
                     /translation="MILRPLHDRVIVKRLDQETKTASGIVLPDAAAEKPDQGEILAVG
                     NGKILDDGRLRPLAVKVHDRVLFGKYSGQAVKIDGQELLVMREEDLFAVVEK"
     gene            1615..>2672
                     /gene="groL"
                     /locus_tag="VFA14_02735"
     CDS             1615..>2672
                     /gene="groL"
                     /locus_tag="VFA14_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828028.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HZS80329.1"
                     /translation="MAAKQVVFGDDARAKIVNGVNILANAVKVTLGPKGRNVVLERSF
                     GAPTVTKDGVSVAKEIELKDKLENMGAQLVKEVASKTSDNAGDGTTTATVLAQAIFRE
                     GVKTVAAGANPMALKRGIDKAVERAVEEIKRLKKDVKGDMIAQVGTVSANGDATIGNI
                     IAQAMDKVGKDGVITVEESKTMETSLEVVEGMQFDRGYLSPYFVTDPERMEAALENPL
                     ILIHEKKISSMKDLLPLLEQVAKMGKPMLIIAEDVEGEALATLVVNKLRGTLNIAAVK
                     APGFGDRRKAMLEDIAILTGGKVISEDLGIKLENVKLDDLGRAKKITIDKDNTTIVEG
                     AGKQSDIEGRVKTLRAQI"
BASE COUNT          692 a          756 c          723 g          501 t
ORIGIN      
        1 actgctgcgc gtcgaagcgg ggcgggcgct ggtggcgccc aacaagatgg ctcaaggttt
       61 ggcgacatat aaagcagcca cgccctggcg tactcgtgca gagcgtggag tccgggccgg
      121 tcatggccag atagcatgca gttcgccgtc tggcaagtgg cgcaccagat ctcccgtgcg
      181 atacctgcgc acgccggcct ggccggcgaa cgggctatgt acaaaacgcc cggcgatcaa
      241 ctcagaccaa ttcatgtaac tgtaaccccg cgccaagcct gcgccggcga tgtaaagctc
      301 gcccaatgcg ccgcggggca ccggctgtag tccgtccgat atattgattt cagtagcgga
      361 taatcctatg tagacaccga catcccgctc gccccaccat aaccggcatc cttgccggag
      421 tacgtcacac cagaaatcca cgcaagagag cgctgatcca cgctcacctc gcaacagtat
      481 tggcactggt agccccgcgc cgaaccttgt tcaacaattg cacaataccg tagagcaaag
      541 cttccgccgt gggaggacaa cccggaacat atacatcgac cggcactatt cggtcacaac
      601 cacgcaccac ggagtaagcg tagtggtagt agccgccgcc attggcacat gaacccatgg
      661 aaatcacaaa gcgcggttca gccatttgat cgtagacctt gcggagcgct ggcgccatct
      721 tgttggtcaa tgtgcctgca acgatcatga catcggactg ccggggactg gcgcgaggca
      781 tgacgccaaa acggtcgaaa tcatagcgcg ccgacgctgc atgcatcatt tcgacggcgc
      841 aacacgccag accaaatgtc atgaaccaca tactgcccgt tcttgcccag tttgcgagat
      901 catccaatcg cgtgaccata tagccatcac gatgaaaact atcaggcaga tcacgatgaa
      961 aactatcagg cagcatatgt caaatctcaa aaaaagcagt cagcgaccgt cggcggtccg
     1021 attcgtcagc ggctcgattc cccggcgcaa ctatagcatc caaatgccga aggcgccaga
     1081 caggcagtac atgaagctgg cgccgcacct gtccaggaga tctctgagcc acgatagcaa
     1141 attccatccc tatctcttga aaaactgcaa cactgtccct atacttagca ctcgtcagga
     1201 aagagtgcta ataattttcc tgacccatgc aggcagtact gcaacactat caattaaatt
     1261 attgaatttt aaggagttcg tatgattctt cgtcctttgc atgatcgcgt catcgtcaaa
     1321 cgtctcgatc aagaaaccaa gaccgcatcc ggtatcgttc tgcctgacgc cgcagctgaa
     1381 aaaccggatc agggcgaaat cctggctgtc ggcaacggca aaattctgga tgatggcaga
     1441 cttcgtccgc tggcagtcaa ggtccacgac cgcgttctgt tcggcaaata ctccggtcag
     1501 gccgtgaaga tcgacggcca ggaactgctg gtcatgcgcg aagaagattt gttcgccgtc
     1561 gtcgaaaaat aagcataccc ccttcaattc aaacgcattt ctggagaatt caacatggca
     1621 gcaaaacaag tagttttcgg cgatgacgca cgtgcgaaaa tcgtcaatgg cgtcaacatt
     1681 ctggcaaacg cagtcaaggt cacactgggt cccaagggcc gtaacgtggt tctggagcgt
     1741 agcttcggcg ccccgaccgt gaccaaggac ggcgtgtcgg ttgcaaaaga aatcgaactg
     1801 aaagacaagc tggaaaacat gggcgcgcaa ctggtcaagg aagtggcttc caagacttcc
     1861 gacaacgcgg gcgacggcac caccaccgca accgtgctgg cgcaggcgat cttccgcgaa
     1921 ggcgtgaaga ccgttgccgc tggagccaac cccatggcgc taaagcgcgg catagataaa
     1981 gccgtggagc gcgctgttga agaaatcaaa cgcctcaaga aggatgtaaa aggcgacatg
     2041 attgcgcagg ttggaaccgt ctccgcaaac ggcgatgcca ccatcggcaa catcattgcc
     2101 caggcaatgg acaaagttgg caaagatggc gttatcactg ttgaagagtc gaagacgatg
     2161 gagacttcgc tcgaggtggt cgaaggtatg cagtttgacc gcggctatct cagtccttac
     2221 ttcgtaactg atcccgagcg catggaagcc gcgctcgaga atccgcttat ccttattcac
     2281 gagaagaaga tcagctcgat gaaggatctg ctgccgctgc ttgagcaggt agccaagatg
     2341 ggcaagccaa tgctgattat cgctgaggac gttgaaggcg aagccctggc caccctggtc
     2401 gtcaacaagc ttcgtggcac gctgaatatc gctgccgtca aggctccggg ctttggtgat
     2461 cgtcgtaagg ccatgctcga agacatcgcg atccttaccg gtggcaaggt gatcagcgag
     2521 gacctgggaa tcaagctcga gaacgtgaag cttgacgacc tcggccgggc caagaagatc
     2581 acaatcgaca aggacaacac gaccatcgtc gaaggcgccg gcaaacagag tgacatcgaa
     2641 ggacgggtca agactctgcg cgcacagatt ga
//
LOCUS       DASRDH010000117         2939 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_477483,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000117 DASRDH010000000
VERSION     DASRDH010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477483"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2866
                     /locus_tag="VFA14_02740"
     CDS             <1..2866
                     /locus_tag="VFA14_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007880283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YhdP family protein"
                     /protein_id="HZS80330.1"
                     /translation="LSNFTVQWEGSYPELQAYRVRGDFSGLGMQAQTARPTQAKTLTR
                     PGHAGVPAIPGFSNLSGTIDATEKGGVLHLASQHAVLHLPTVFADFAPDFDILNLRSR
                     WEMQQSETVLIHLDSMAFAQEGIAGTLSGTHLMPLRGTSLGVIDMKATLSTFDLRKLK
                     RFLPLHTPSPVSDWVAGALLDGRASDVEIAAKGALADFPFHNPGPDGKSMGTFRLSGR
                     FEKLKLDYAPSHIGQGGKSPEWPLLEDASGKLSIDHARLEVVADAGKTHGIAVSNVKA
                     TIPDLLDAESSLEIEGNAAGAMQDFLRYVQDSPVAHWIDHFTEDSKSTGNARLHLKFQ
                     LPLHHMIDAKVNGSLQFIDNDVSLLAGLPTVYRTSGKLEFNEKGFNLPAIEGVFLGGR
                     VTLAGGIRSDGSTLVRLDGTLTTDGVRQAYANTGPVLQRMLEHVSGAARYSGTIAIRE
                     HQPEIMIESSLQGLALNLPAPLRKTANESWPLKFELVGLNGGNTSIERDEIKLSLGTL
                     MSARYRRQKAPGKQWEVQSGGIGIGRPAPQPDNGLVMYADAENMNLDRWTAFRASMLQ
                     AANGSASRVDAGIDAYLEPDAIALRATELVVMGKKLDNVIVGASHQHGSWQVNIASDQ
                     ASGYATWNEPSSGHRMGRIMARLAMLNIPEGAVQDAGHVLEGDGDTADKISSLDIVVE
                     DFRLSGKKLGRLELDADNARVSAGSEWRINKLSLINPDAELRAAGNWVALDKSNTSSL
                     SYTLNIDDAGKLLDRFGFPGVVKGGKGKLDGDITWKGLPFSLDIPTLGGRISMNVHTG
                     QFLKVNQGVAKLLNVLNLQALPRRLALDFRDIFSKGFAFDSIIGAASIAQGIVTTDDL
                     KMTGVAASVLMNGSADIARETQNLRLVVIPEVNLGTASLLALAVNPAVGIGTFVAQLF
                     LRDPLMKTLTFGYHVSGSWSDPQIVRQEHAGGADDRPVSE"
BASE COUNT          647 a          862 c          876 g          554 t
ORIGIN      
        1 actcagcaac tttaccgtcc aatgggaggg cagttatccg gaactacagg cgtatcgcgt
       61 gcgcggagat ttcagcggtc tgggcatgca ggctcagacc gcgcgtccta cccaggcgaa
      121 aaccttgacc cggcccggtc atgccggcgt accggcgatc cctggttttt ccaatctgag
      181 cggaaccatc gatgccacgg aaaaaggcgg tgtgctgcac ctggcgtcgc aacatgccgt
      241 actgcatttg ccgacggtgt ttgccgactt cgcgccagat ttcgacatct tgaacctgcg
      301 cagtcgctgg gagatgcaac agagcgagac cgtgctgatt catctcgaca gcatggcatt
      361 cgcgcaggag ggcatcgccg gtacgctgag tggcacgcat ctgatgccgt tgcggggaac
      421 atcgctcggc gtcatcgata tgaaagcgac actgtcgact ttcgatttac gcaagctcaa
      481 gcgtttcctg ccactgcata ctcccagtcc ggtatcggac tgggttgccg gcgctctgct
      541 ggatggccgc gccagcgatg ttgaaatcgc agccaagggc gcgctggcag atttcccttt
      601 ccacaatccc ggaccggatg gcaaaagcat gggtacattc aggctttccg gcagattcga
      661 aaaactgaag ctcgattatg ccccatcgca tatcggccag ggtggcaaat cgcctgagtg
      721 gccgctgctc gaagacgcca gcggcaaact atcgatcgac cacgcgcgtc tggaggtcgt
      781 cgccgatgcc ggcaagaccc atggcattgc cgtatcgaac gtgaaggcga ccattcctga
      841 tctgctcgac gctgaatcct cgctggagat cgaaggtaat gccgccggcg cgatgcagga
      901 ctttctgcgc tatgtccaag acagtccggt ggcacattgg atcgaccatt ttaccgaaga
      961 cagcaagtcg accggcaacg ccagattgca cttgaaattc cagttgccgc tgcatcatat
     1021 gatcgatgcc aaggtcaacg gcagcctgca gttcatcgac aacgatgtgt ctttgctggc
     1081 gggcctgcca acggtatacc ggaccagtgg caaactggag ttcaacgaaa aagggttcaa
     1141 tctgccagcc atcgagggcg tgttcctggg cgggcgggtg acgcttgccg gcggtatccg
     1201 aagtgacggc agcacgctgg tcagactcga tggcacgctc accaccgacg gtgtgcgtca
     1261 agcctatgcc aacacaggcc cggtgttgca acgcatgctg gagcacgtga gcggcgcggc
     1321 acgttacagc gggacgatcg ccatacgcga gcatcagccg gaaatcatga ttgaatctag
     1381 cctacaaggt ctggcattga atctgccggc gccgttgcgc aagacggcga atgaaagctg
     1441 gccgctgaag ttcgaactgg tcggcctgaa cggcggcaat acatcgatag aacgtgatga
     1501 aatcaagctg tcactgggaa cgctgatgtc agcgcgctat cgccggcaaa aggcgcccgg
     1561 caaacagtgg gaggtacaaa gcggcggcat cggcatcggc cggccggcgc cgcaacctga
     1621 caacggcctg gtgatgtacg ctgatgccga gaacatgaac ctcgatcggt ggacggcgtt
     1681 ccgcgcctcg atgctccaag cagccaacgg cagcgcaagt cgcgtcgatg ctggcatcga
     1741 tgcttatctg gaaccggatg cgatcgccct gcgcgcgact gagctggtag tgatgggcaa
     1801 aaaactcgac aacgtgatcg tcggcgcctc gcatcagcac gggagctggc aagtaaatat
     1861 tgcctcggat caggccagcg gttacgccac ctggaatgaa cccagcagcg gccaccggat
     1921 gggccgcatc atggcgcggc tggcgatgct gaacattccg gagggcgcag tccaggacgc
     1981 tggccatgta ctcgaaggcg atggcgatac tgccgacaaa atttcgtcgc tcgatatcgt
     2041 cgttgaagat tttcgactgt ctggcaaaaa gctcggccga ctggaactcg atgccgacaa
     2101 tgcgcgcgtc agcgccggca gcgaatggcg catcaacaag ctgagcctga tcaatcccga
     2161 cgccgagcta cgcgcggcgg gcaactgggt ggcgctcgac aagagcaaca cctccagtct
     2221 gagctatacg ctgaacatcg atgacgccgg taaattgctg gaccgtttcg gtttccccgg
     2281 cgtggtcaaa ggcggcaagg gcaaactgga tggcgacata acctggaaag gtttgccgtt
     2341 ctcgctggat ataccgacgc tgggcggccg gatcagcatg aacgtacata ccggacaatt
     2401 ccttaaagtt aatcagggtg tggccaaact gctcaacgta ctcaatctgc aagccttgcc
     2461 gcgtcggctg gcgctggatt tccgcgatat cttctctaag ggattcgcct tcgacagcat
     2521 catcggcgca gcctccatcg cccagggcat cgtcacgacc gacgatctga aaatgacagg
     2581 cgttgcggca tcggttttga tgaacggttc cgccgatatc gcccgggaaa cgcaaaacct
     2641 gcgcctggtg gtcattcccg aagtcaatct cggcacggca tccctgctgg cactggcggt
     2701 caacccggcg gtcggcatcg ggactttcgt ggcgcaacta ttcctgcgcg acccgctgat
     2761 gaagacgctg acctttgggt atcatgtcag cggctcctgg agcgatccgc agatcgtcag
     2821 acaagaacat gccggcggcg ccgacgacag accggtatcg gaatgacggc atgcgcccgg
     2881 taacatcgtc ccccatcgtc tacaatacgt ccatgaaaac actcgaaccc aatctcagc
//
LOCUS       DASRDH010000118         2192 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_98364,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000118 DASRDH010000000
VERSION     DASRDH010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98364"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..160
                     /locus_tag="VFA14_02745"
     CDS             <1..160
                     /locus_tag="VFA14_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014725605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HZS80331.1"
                     /translation="VYEFVKHDDGADRLQINAQNCVHCKTCDIKDPTQNIVWVAPEGG
                     GGPNYPNM"
     gene            293..1621
                     /locus_tag="VFA14_02750"
     CDS             293..1621
                     /locus_tag="VFA14_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006390014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HZS80332.1"
                     /translation="MSRITTALPDAPTGTVGGRYRLFIVVLIVVALAESIGASSFRLG
                     PGKIVLLPMLWALLIAAAWGIAQRRVPACMRIQPALQAYAGGLLNAGLLLFIVKLGLT
                     VGSALPEVRQAGWALLFQEFGHAFGTLVLGLPLALLLGIKREAVGATFSVGREGNLVI
                     IGQKYGMASPEGRGVLAEYITGTVLGALFIALLAGFITSLHIFDPRSLAMGAGVGSGS
                     LMAAAIGAIAAQQPGAMAPELTAIAAASNLLTMVVGFYFTLFLSLPAASWLYDRLEPV
                     LGRFSKRKLIETAATASADGAIQELSLADKAISWMLIAAGVTIGNWLSFKVAPIQSLI
                     GTAVIAFIVAIVEIARCRMPKMPSILVLSIIATTVGVPGLFPFSDTIIALTGQLNFLA
                     FTTPVLALAGFSVAKDLPLFRQLGWRIVVVSLMATAGTFLGATLIAELFH"
     gene            1659..>2192
                     /locus_tag="VFA14_02755"
     CDS             1659..>2192
                     /locus_tag="VFA14_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HZS80333.1"
                     /translation="MYPDPDIAADTRARMLAWRHDIHAHPETAFAEHRTANIVAQALE
                     TMGIVVHRGLAGTGVVGALKNGDGPSIALRADLDALNMQELGSHAHKSTCNGKMHGCG
                     HDGHTAMLLGAAEHLSRHKPFKGTVYFIFQPAEENEGGGRKMVDEGLFSLFPADAVYG
                     MHNFPNLPRGRFGIRTGT"
BASE COUNT          409 a          717 c          623 g          443 t
ORIGIN      
        1 agtgtatgag ttcgtgaagc atgacgacgg cgccgaccgt ctgcaaatca atgcgcagaa
       61 ctgcgtgcat tgcaaaacct gcgatatcaa ggatccaacg cagaatatcg tgtgggtggc
      121 gcctgagggc ggcggcgggc caaactatcc gaatatgtga tccgttcatc caacggtcgc
      181 cggatcaatt gctgataaac ttgccacaca acaattcaag gtctttaccg gaccagcata
      241 cggacaatcg ctcgctgccg ttcgtcatgc aatttttctt ttggagacac tgatgtcacg
      301 tattaccact gccttgcctg acgcgcccac cggcaccgtc ggtggcagat accggctttt
      361 catcgtggtg ctgatcgtgg tcgcgctggc ggaatccatc ggcgcaagta gtttcaggct
      421 aggccccggc aaaatcgtgc tgctgcccat gctctgggca ctgctcattg cggcggcctg
      481 gggcatcgcg cagcggcgtg taccggcatg catgcgcatc cagccggctc tacaagccta
      541 cgctggtggc ctgctcaatg cgggcctgct tttatttatc gtcaagctcg gcctgaccgt
      601 cggcagcgcc ttgccagaag ttcgccaggc tggctgggcg ctgctgtttc aggagttcgg
      661 ccacgccttc ggcacgctcg tgctcggatt accgctggcg ctactgctcg gcatcaagcg
      721 cgaagccgtt ggcgcgacct tttcagtcgg acgcgaaggc aatctggtca tcatcggcca
      781 gaaatacggc atggcttcgc ccgaaggccg tggcgtgctg gctgaataca tcactggcac
      841 ggtactcggc gcattattca tcgcgctgct cgccggtttc atcaccagcc tgcacatctt
      901 cgatccgcgc tcgctcgcca tgggcgccgg cgtcggctcg ggcagcctga tggcagccgc
      961 catcggcgcc atcgcggcgc agcagccggg cgccatggcg cccgaactca ccgccattgc
     1021 cgcggcatcg aacctgctga ccatggtagt cggcttttac tttacgctgt tcctgtcgct
     1081 gccagcagcc tcctggctgt atgacaggtt agagccggtg ctgggacgct tctcaaaacg
     1141 caaactgatc gagacggcgg caacggccag cgccgatggc gcaatacagg aactgtcact
     1201 ggccgacaaa gccatctcgt ggatgctgat cgccgcgggg gtaacgatcg gcaactggct
     1261 ttcgttcaaa gtggcaccga tacaatccct gatcggcacg gcggtcatcg ccttcattgt
     1321 cgcgatcgtc gaaatcgcca ggtgccggat gccgaaaatg ccctccatcc tcgtgctgtc
     1381 catcatcgcc acgacagtcg gcgttccggg cctgttcccc ttctccgaca ccatcatcgc
     1441 cttgaccggt caactcaatt tcctcgcatt caccacacca gtgctggccc tggccggctt
     1501 ctcggtcgcc aaagacctgc cgcttttccg gcaactgggc tggcgtatcg tggtcgtctc
     1561 cctgatggcg accgccggta ccttcctcgg cgcgacattg atcgccgaat tgttccattg
     1621 atcccgcaaa cctgacaaca ctctttacgg agaagctcat gtatcctgac cctgacattg
     1681 ccgcagacac ccgcgcccgc atgctggcct ggcgccacga catccacgcc catccggaaa
     1741 ccgctttcgc ggaacatcgc acggccaaca tcgtcgcgca ggcccttgaa acgatgggca
     1801 tcgttgtgca tcgcggactc gccggcaccg gtgtggtcgg cgccctcaag aacggcgatg
     1861 gccccagcat cgccttgcgc gccgatctcg atgctctcaa catgcaagaa ctcggcagcc
     1921 atgcgcacaa atcgacctgt aacggcaaga tgcatggctg cggccacgac ggccacaccg
     1981 ccatgctgct cggcgcggcg gagcatctgt cgcgccacaa accgttcaaa gggacagtgt
     2041 atttcatctt tcagccggcc gaagagaatg aaggcggcgg ccgcaagatg gtggacgaag
     2101 gcctgttcag cctgttcccg gccgatgccg tctatggcat gcacaatttc ccgaacctgc
     2161 cgcgcggcag gttcggcatc cgcaccggca cg
//
LOCUS       DASRDH010000119         2735 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_359648,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000119 DASRDH010000000
VERSION     DASRDH010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2735
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_359648"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="VFA14_02760"
     CDS             <1..335
                     /locus_tag="VFA14_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HZS80334.1"
                     /translation="NERKIQQFGHHVFESPAHIVTHAGKNTVLTQKEFALAILLFSNL
                     GQPLSRAYLQENIWGLEDRDELPPRTIDTHVSRVRNKLNLRPENGFRLGTIYGYGYQL
                     EKLTGSAD"
     gene            490..1797
                     /gene="hemA"
                     /locus_tag="VFA14_02765"
     CDS             490..1797
                     /gene="hemA"
                     /locus_tag="VFA14_02765"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017449704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HZS80335.1"
                     /translation="MHLFAVGLNHITAPLSLREKLAFPADQIGQAVAAARAWFGRTNL
                     SSTSAQTGEAAILSTCNRTELYATSTLTGGSGAVDAAIDSTAHFLTDYHKLSYAELRP
                     FLYALRQDDAVRHAFRVASGLDSMVLGEPQILGQMKNAVRQADAAGGLGTHLHQLFQR
                     TFAVAKEVRSTTGIGAHSVSMAAAAVRLSQRIFDTVSGQNVLFIGAGEMIELCATHFA
                     AQNPKSLTVANRTLDRGAALAHRFNGSSIRLADLPTQLSKFDIVVSCTASSLPIIGLG
                     LVERAIKVRRHKPMFMVDLAVPRDIEAEVACLNDIFLYTVDDLASVVQSGVENRQAAV
                     AQAEAIIETRVQSFMHWIDSRAVVPVIRDLHDGSETLRLAELERARKMLVRGENIDVV
                     LDALSRGITAKFLHGPQQALRHAQGSERARLVKLLPQLFRTKR"
     gene            1894..>2735
                     /gene="prfA"
                     /locus_tag="VFA14_02770"
     CDS             1894..>2735
                     /gene="prfA"
                     /locus_tag="VFA14_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017449705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HZS80336.1"
                     /translation="MKASMLARLDQFSERLEELNHLLAQEDATSDIDNYRKMTREHAE
                     LGPLVALHHVRTQVVKDITTAQELLTDPDMKEFAQEELNTLRTHMTALESDLQKMLLQ
                     KDPNDERNIFLEIRAGTGGDESALFAGDLLRMYTRFAERHRWQVEIVSASEPEIGGYK
                     EVIVRIAGFGAYSRLKFESGGHRVQRVPATETQGRIHTSACTVAVMPEADEVEDVAID
                     PADIRIDTYRASGAGGQHINKTDSAVRITHVPTGIVVECQNDRSQHKNKASALKVLAA
                     RIKDA"
BASE COUNT          600 a          846 c          742 g          547 t
ORIGIN      
        1 acaatgaacg caagattcag caattcggcc atcatgtttt cgagtcgccc gcgcatatcg
       61 tcacccatgc cggcaaaaat acggtgttga cgcaaaagga atttgcgctg gcgatcctgc
      121 ttttcagcaa cttgggccag ccgctgtcgc gtgcctattt gcaggaaaac atctggggac
      181 tggaagacag ggatgaactg ccgccccgaa ccatcgacac ccatgtctca cgcgtgcgca
      241 acaaactgaa tcttagaccg gaaaacgggt ttcggctcgg cacgatatat ggctacggtt
      301 atcagctgga aaaattgaca ggctctgcgg actgagacat tgccgctttt gttatcaaac
      361 aggaaatgac tgtcccgact aatagcctga ctatatagtc actattgata cactccaaaa
      421 tcccttgaga tacgatgggt ataatatctc atcggtactc ccctatttcg cacgctgctg
      481 cccgtcacca tgcacctgtt tgccgtcgga ctgaaccata tcaccgcgcc gctttccctg
      541 cgcgaaaagc tagccttccc ggctgaccag atcggtcagg cggtggcggc agcgcgcgcg
      601 tggttcggtc gtacgaattt gtcatccacg tcagcgcaga ctggcgaagc ggcgattctg
      661 tcgacttgca accgcaccga gctgtacgcg accagcacgc tgaccggcgg cagcggtgct
      721 gtcgatgccg ccatcgacag cactgcgcat ttcctcaccg attaccacaa actttcttat
      781 gccgaactgc gtccttttct gtatgcctta cgccaggatg acgccgtgcg ccatgccttc
      841 cgcgtcgcct ccgggctgga ttcaatggtg ctgggcgagc cgcagattct cggccaaatg
      901 aagaacgccg tgcgtcaggc ggatgccgcc ggcggacttg gcacacattt gcaccaattg
      961 ttccagcgta cctttgcagt cgccaaggaa gtacgttcga cgaccggaat cggcgcgcat
     1021 agcgtgtcga tggccgctgc cgccgtgcgt ttgtcgcaac ggattttcga tacggtttcc
     1081 ggccagaacg tgctgtttat cggcgccggc gaaatgatag agctgtgcgc gacgcatttt
     1141 gccgcacaaa atccgaaatc gctgaccgtc gccaaccgca ccctggatcg cggtgcagcg
     1201 ctggcgcatc gcttcaacgg ttcgtcgatt cgtctagccg atttgccgac gcaattgtcg
     1261 aaattcgaca tcgtggtatc ctgcaccgcc tcgtcgttgc cgatcatcgg tctcggccta
     1321 gtcgaacgcg ctatcaaggt gcgccgccac aaaccgatgt tcatggtcga cctggccgtg
     1381 ccacgcgata tcgaagccga agtcgcatgc ctgaacgaca ttttcctgta caccgtcgat
     1441 gatctcgcct cggtcgtgca aagcggcgtt gaaaatcgcc aggctgccgt tgcgcaggcc
     1501 gaggccatca tcgaaacccg tgtgcaatct ttcatgcact ggatcgatag ccgcgccgtc
     1561 gtgccggtga tccgcgattt gcacgacggc agcgaaacgc tgcgcctggc agaactggaa
     1621 cgcgcgcgta aaatgctggt caggggcgaa aacatcgacg tcgtgctcga cgcactgtcc
     1681 agaggcatca ctgctaaatt cctgcacggc ccgcagcagg ctttgcgcca tgcgcaaggc
     1741 agcgagcgtg cccgactcgt caagctgttg ccacaactgt tccgcaccaa acgttagcga
     1801 cgtcgttcgc ttacggattg cactgcatgc aatcgcttcc ctgctcgccg ctgcgtgcgc
     1861 tgcttcacca ctccattcac cccaggcatt tccatgaaag catccatgct cgccagactc
     1921 gaccagttct ccgaacgact ggaagaactc aatcatctgc ttgcgcagga agacgccacg
     1981 tccgacatag acaattatcg caagatgaca cgcgagcatg ccgaactcgg tccgctggta
     2041 gcgctgcacc acgtccgcac gcaggtcgta aaggacatta ccaccgcgca ggaattgctg
     2101 acggatcctg acatgaagga attcgcccaa gaggaactca ataccctcag gacacacatg
     2161 acagcactgg aaagtgatct gcaaaagatg ctgctgcaga aggatcccaa cgacgagcgc
     2221 aatatcttcc tcgaaatccg cgccggtacc ggcggcgacg agtcggcatt gtttgccggc
     2281 gacctgctgc gcatgtacac gcgctttgcc gaacggcatc gctggcaggt cgagatagtg
     2341 tccgcatccg aaccagaaat cggcggctat aaggaagtca tcgtgcgtat cgccggtttc
     2401 ggcgcgtact cgcgtctgaa attcgaatcc ggcggccacc gcgtgcagcg tgtgccggcg
     2461 accgaaaccc aaggccgcat ccatacctcc gcctgtaccg tcgccgtgat gccggaagcc
     2521 gatgaagtcg aagatgtggc catcgatcca gccgatatcc gcatcgacac ataccgcgcc
     2581 tcaggcgccg gcggccagca tatcaataaa accgactcag ccgtgcgcat cacccacgtg
     2641 ccgaccggca tcgtggtgga atgtcagaac gaccgtagtc agcacaagaa caaggcctct
     2701 gcattgaagg tgctggcggc gcgcatcaag gacgc
//
LOCUS       DASRDH010000120         2338 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_770514,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000120 DASRDH010000000
VERSION     DASRDH010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_770514"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..678
                     /locus_tag="VFA14_02775"
     CDS             <1..678
                     /locus_tag="VFA14_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013232737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HZS80337.1"
                     /translation="VIQAEGGVFSEQTARARQRDYDQVAIITHVKTSARIPHRAFERF
                     TAEGPLALLPQDDGYALVWCTRPASAQALLALDDQAFLHALGETFGERVGRFTACDAR
                     KSYPLGLNAEPGMSARTVAIGNAAQTLHPVAGQGLNLGLRDAVTLASALTRNACDPAA
                     LVQFARQRRRDRNAVINSTDLMARVFASAPDGAPAQTLLGLSLGLIDVITPVKRLLAE
                     RMMFGWR"
     gene            861..>2338
                     /locus_tag="VFA14_02780"
     CDS             861..>2338
                     /locus_tag="VFA14_02780"
                     /inference="COORDINATES: protein motif:HMM:NF019291.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HZS80338.1"
                     /translation="MLQQLDKADTVRRMVAPHTTQKHKSEFGQFMTPSSVARFMASLF
                     PISTLQTCRLLDAGAGVGALSCAFLDRWVTGGFGFGAVDATAYEIDDNLHDHLTQHLA
                     GYQGVTPRVITGDYIELATADNLFTAKQISGYTHAILNPPYKKINSSSAHRLALRRVG
                     IETVNLYSAFVALAVTQAAPNGQIVAIIPRSFCNGPYYRPFRDFILERAAIRHMHLFE
                     SRNKAFKDDDVLQENIIIRLERDGQQGPVAVSTSTDDTFIDLVTHEHPFDRIVFPDDP
                     ERFIHVPTSPEKNAIESSSAIRYVLADLGVKVSTGPVVDFRLKEHLRDMPGKNTVPLL
                     YPGHFSSSGTTWPIEGMKKPNAIERNADTEKWLYPSGFYCVVRRFSSKEEKRRIVASV
                     LEPVAFGDAPVLGFENHLNLFHENKRGLPEALARGLAVFLNTTAVDEHFRRFNGHTQV
                     NATDLKLMKYPSRDALIQLGEWVVGQGTLTQDQIDARLGTLAA"
BASE COUNT          485 a          720 c          670 g          463 t
ORIGIN      
        1 gtgatacagg cggaaggcgg cgtgttctct gagcagacag ccagggcacg gcaacgcgac
       61 tatgatcagg tcgccatcat cacgcatgtg aagacctctg cccgcatccc ccatcgcgcc
      121 ttcgagcgct ttaccgccga agggccgctg gccttattgc cgcaagatga cggttatgcg
      181 ctggtgtggt gtacccggcc ggcaagcgcc caggcgctgc tggcgctcga cgaccaggca
      241 tttctgcatg ctctcggtga gacattcggc gagcgcgtcg gccgctttac tgcatgtgac
      301 gcgcgcaaga gttatccgct cggactgaat gccgaacccg gcatgtcggc gcgcacggtc
      361 gccatcggca atgccgcgca aaccctgcac ccggtcgccg gccagggcct caacctgggg
      421 ctgcgcgacg ctgtgacgct ggccagtgcg ctcacccgca acgcctgcga cccggccgcg
      481 ctggtgcaat tcgcccgcca gcgccgacgc gaccgcaacg ccgtcatcaa ctcgaccgac
      541 ctgatggcgc gtgtcttcgc cagcgcaccg gacggcgcgc cggcgcagac attgcttggt
      601 ttgtcgctgg ggctgatcga cgttatcacg cccgtcaaac gcctgctggc ggaacgcatg
      661 atgttcggct ggcgttgaga tcagccgtat cacccaggaa ggttgtccat aaaacggtgg
      721 ttttctgcac agcctgcaat gcttggattt tcgcgtgacc acaagccctt gcgatgttaa
      781 catacccgtc aatttttcaa tgctgtggta tcgaccatga gtcaacacat agatgatgcc
      841 gactacgccg aggagcggaa atgctgcaac agcttgacaa ggcagacacc gtgcgccgga
      901 tggttgcgcc gcacactact caaaagcata aatctgagtt cggtcagttc atgacacctt
      961 cgagtgtggc ccgcttcatg gcgtccctgt tcccaatcag tactttgcag acctgccgcc
     1021 tattggatgc cggcgcgggc gttggtgcgc tgtcctgcgc ctttctcgac cgctgggtaa
     1081 cgggtggctt tggttttggc gctgtcgacg cgactgccta cgaaatcgac gacaatttac
     1141 acgatcacct tacacaacac ttggctggat accaaggcgt gactccccgg gtcatcacgg
     1201 gcgattacat tgagctggcg accgctgaca atctgttcac cgcgaagcaa atctcgggct
     1261 acacgcacgc catcctcaat ccgccctata agaaaatcaa tagcagttcc gcacaccggc
     1321 tggccctgcg tcgtgtcggc atcgagacgg tgaacctgta tagcgccttc gtggcgctgg
     1381 cagtcaccca ggcagcgcca aacgggcaga tcgtagccat catcccgcgc agcttctgca
     1441 atgggccgta ctataggcca ttccgcgatt tcatcctcga gcgtgcggcc attcgccaca
     1501 tgcacctgtt cgaatcgcgc aacaaggcat tcaaggatga tgatgttctg caagagaaca
     1561 tcatcatccg cttggaacgg gacggtcagc agggaccggt ggcggtttca acatcaaccg
     1621 atgacacctt catcgacctt gtgacccatg aacacccatt cgaccggatc gtgttcccgg
     1681 atgacccgga gcggtttatt cacgtcccta cgtccccgga gaaaaacgcc atcgagtcat
     1741 cctcggcaat ccgctatgtg ctggccgacc tgggcgtcaa agtatcgacc gggccagtag
     1801 tcgatttccg actgaaagaa cacctgcgcg acatgccggg gaagaacact gtgcctttgt
     1861 tgtatcccgg tcatttcagc agcagcggca ctacgtggcc catcgaaggg atgaagaagc
     1921 cgaatgctat cgagcgcaat gccgacacgg aaaaatggct gtacccgagc ggcttctact
     1981 gtgtggtgcg tcgcttctcg tccaaggaag aaaagcgccg gatcgtggcg agcgtacttg
     2041 aaccggtggc attcggcgac gcgccggtgc tgggtttcga gaaccacctg aatctgttcc
     2101 acgagaacaa acgtggcttg cccgaggcgc tggcccgtgg actggcagta ttcctgaaca
     2161 cgaccgcagt agacgaacat ttccggcgct tcaacggcca cacccaggtg aatgcgactg
     2221 atctcaagct aatgaaatat ccgagccgtg acgccctgat ccagcttggt gagtgggtag
     2281 ttggtcaagg aacgctcacg caagaccaga tcgacgccag gttgggaact ctcgccgc
//
LOCUS       DASRDH010000121         2935 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_126012,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000121 DASRDH010000000
VERSION     DASRDH010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2935
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126012"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..784)
                     /locus_tag="VFA14_02785"
     CDS             complement(<1..784)
                     /locus_tag="VFA14_02785"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HZS80339.1"
                     /translation="MKDERMRCVGAGLILMCAGMVSPVIAGDLMDWMTAPFADPLSSR
                     PPQLDSGARLPGDVVSVRCDDGVGSASEEISLLDAVHLGLCRDPQIHGAWASVKVQAA
                     QAGGARAAYLPTIAAGISRQHQQSSHPALRFAGNSGITQDARYVTLTWRLLDAGARGA
                     HRRTADALLAAALANHDAALQKTLANVIGVYFDAQIARAGRESAARGEALARQTLQIA
                     IRRESQGIGARTNILQAQTALAKAELERSRTQGRYQKALAALR"
     gene            complement(774..2117)
                     /locus_tag="VFA14_02790"
     CDS             complement(774..2117)
                     /locus_tag="VFA14_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013209362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HZS80340.1"
                     /translation="MSLPHRRQAWGVLLRRYAGVFSHSWKMRHHLRTDFFNKQEAEFL
                     PAGLALQEMPESASLRWTGRILMALVLFALLWSILGHVDIVVNAAGKVIPSARTKTIG
                     SVDVASVRALHVVEGQAVRAGDVLIELDSSASDAEHDKARDVLIQARLQVARSQAMID
                     AVERLQAPALAAVAESTNSQWQAARRQLDGQYRDFRARLARLDGDIAHYAQALPLAAQ
                     RAEDFKALLPERMVSRHAWLEKEQARVDLEGQLAQARQQRAVLIAQTRREAHDALIDG
                     EKIIAAAGQDQRRAGEHSRLLKLTAPVDGTVQQLLVHTVGGVVPAAQPLMQIVPRESV
                     VEIEAFLDNKDVGFVQIGQRAQVKIDAFNYTKYGSIAATVRHVSRDAIEDDKRGLIYA
                     TRIVLDKNSLVVDGKIMPLSPGMSVTAEIKTGDRRIIEYVLSPLQRHQREALHER"
     gene            complement(2114..>2935)
                     /locus_tag="VFA14_02795"
     CDS             complement(2114..>2935)
                     /locus_tag="VFA14_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006462999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZS80341.1"
                     /translation="RAEVSGNKTRMPRVAGLIEFDQVSFRYRPDAPDVIRALSLKIRP
                     GEVIGIVGRSGSGKSTLTKLVQRLYVPDRGHVSVDGQDIAIIDTASLRRQIGVVLQEN
                     TLFTRSIRDNIALSDPALLIESIIAAARLAGAHGFICELPEAYDTLVGEHGIGLSGGQ
                     RQRIAIARALITNPRILIFDEATSSLDYESEKIIQDNMRQICAGRTVLIIAHRLSAVR
                     DASRIVVMERGQIAESGSHDELLRHPGGIYRHLYQLQSSQRPSSAAHADAALVSP"
BASE COUNT          566 a          988 c          818 g          563 t
ORIGIN      
        1 tccgcaatgc agccagcgcc ttttggtatc ttccctgggt ccggctgcgc tccagctcag
       61 cctttgccag agctgtttgc gcctgtaata tattggtgcg tgcgccaatg ccctgggact
      121 cccgcctgat ggcaatctgc agggtctgcc gcgccagcgc ttccccgcgt gctgccgatt
      181 cgcggccggc cctggcgatc tgcgcatcaa agtacacgcc gatcacgttc gcgagcgtct
      241 tttgcagggc agcgtcatgg ttcgccaatg cagcggccag caaggcatcc gccgtgcggc
      301 gatgcgcgcc gcgtgcgccg gcatcgagca accgccaggt caacgtgaca tagcgcgcat
      361 cctgtgtgat gccgctgttc ccggcaaagc gcaacgccgg atgactggac tgctgatgct
      421 ggcgactgat gccggcggcg atcgtcggca gatatgccgc gcgcgcaccg ccggcctgcg
      481 ccgcctgcac cttcacggat gcccaggcac catgaatctg cggatcgcgg cacagaccca
      541 gatggactgc atccagtaaa ctgatctctt cactggcact gccgacgccg tcatcacaac
      601 gcacactcac cacatcaccg ggcaatctgg cgccactgtc caattgtggc ggacgactcg
      661 agagcggatc ggcgaacggc gccgtcatcc agtccatcag atccccggca atgacgggcg
      721 aaaccatacc cgcgcacatc aggatcaggc cggcgccaac gcatcgcatc cgctcatctt
      781 tcatgcagcg cctcgcgctg gtgccgctgc aatggtgaaa gcacgtattc gatgatcctt
      841 ctatcaccgg ttttgatttc ggcagtgacc gacatgcctg gcgacagcgg catgattttt
      901 ccatccacga ccaaactgtt tttatccaga acgatgcggg ttgcgtaaat cagtccccgt
      961 ttgtcatctt cgatggcatc ccgcgagaca tgcctgaccg tcgccgcgat gctgccgtat
     1021 ttggtgtagt tgaaggcatc aatcttgacc tgcgcacgct ggccgatctg cacgaaaccg
     1081 acatccttgt tatccagaaa agcttcgatt tcgaccacgc tctcgcgtgg cacgatctgc
     1141 atcaatggct gggccgcagg caccacgccg cccacggtat gtaccaacaa ttgctgcacc
     1201 gtgccatcca ccggcgccgt cagtttcagg agcctgctgt gctcgcccgc gcgccgctga
     1261 tcctgcccgg cagcggcgat gatcttttcg ccatcgatca aggcgtcgtg ggcctccctg
     1321 cgtgtctgcg cgatcagaac cgcacgttgc tggcgcgctt gcgccaattg cccctccaga
     1381 tccaccctgg cttgctcctt ctccagccag gcatgacgcg acaccatgcg ctccggcagc
     1441 agcgccttga aatcttccgc ccgctgcgcg gccagcggca aagcttgcgc atagtgcgcg
     1501 atgtctccat cgagtcgggc aagccgggca cgaaagtcgc gatactgacc atcgagctgt
     1561 cgtcgcgctg cctgccactg actgttcgta ctctccgcca ctgccgccaa tgccggcgcc
     1621 tggagccgct ccacggcatc gatcatggcc tgcgaccgcg caacctgaag tctggcctga
     1681 atcagcacat cacgggcctt gtcgtgctcc gcatcgctgg cgctggaatc cagttcgatc
     1741 agcacatccc cggcccgcac cgcttgccct tcgacgacat gcaaggcgcg cacactggcg
     1801 acatccactg aaccgatggt cttggtacgc gcggaaggaa tcaccttgcc tgcggcattc
     1861 accacaatat cgacatgacc caaaatcgac cagagcaggg cgaacagcac cagcgccatc
     1921 agaatgcgtc cggtccagcg caacgacgcc gattccggca tctcttgcag cgccagcccg
     1981 gccggcagga attccgcctc ctgcttattg aaaaaatccg tgcgcaagtg atggcgcatt
     2041 ttccaggaat gggaaaatac gccggcgtaa cgtcgcagca acactcccca tgcctgccgg
     2101 cgatgaggca ggctcatggc gataccaatg ctgcgtccgc gtgcgctgcc gaagacggcc
     2161 gctggctgct ttgcagctga tacaggtgac ggtagatacc gccggggtga cgcaacagtt
     2221 cgtcatggct gccgctttcg gcgatctggc cacgctccat caccacgata cgactggcgt
     2281 cgcgcaccgc actcaagcga tgcgcaatga tgagcacggt acgtccggca caaatctgac
     2341 gcatattgtc ctggatgatt ttttcggatt cgtaatccag ggagctggtc gcttcatcga
     2401 aaatcaaaat gcgcggattg gtgatcaagg cccgcgcgat cgcaatccgc tggcgctggc
     2461 caccggacaa accaataccg tgctcaccca ccagcgtatc gtaagcttcc ggcagttcac
     2521 agatgaagcc gtgcgctccc gccagcctgg ccgccgcgat gatgctctcg atcagcagcg
     2581 ccggatcgga cagcgcgata ttgtcgcgga tggagcgcgt aaacaaggta ttctcctgta
     2641 acacgacgcc gatctgccgg cgcagggagg cggtgtcgat aatcgcgata tcctgcccat
     2701 ccacactcac gtggccgcga tccggcacat acaggcgctg caccagcttg gtcaatgtac
     2761 tcttgccgga tcctgaacgt ccgacgatac cgatcacttc tccaggacga atcttcaggc
     2821 tcagcgcccg gatcacatcc ggcgcatcag ggcggtaacg gaacgacacc tggtcgaatt
     2881 cgatcagtcc cgccacgcgc ggcatgcggg tcttgttacc gctcacttcg gcacg
//
LOCUS       DASRDH010000122         2373 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1171291,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000122 DASRDH010000000
VERSION     DASRDH010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1171291"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..622)
                     /gene="dapE"
                     /locus_tag="VFA14_02800"
     CDS             complement(<1..622)
                     /gene="dapE"
                     /locus_tag="VFA14_02800"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008450200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HZS80342.1"
                     /translation="MSKTLVLAQQLIALSSVTPEDKGCQARLAELLTPLGFVCETIQS
                     GAVTNLWARKGQAQPLLTFAGHTDVVPTGPASEWQSPPFVPTQRNGKLFGRGAADMKT
                     SIAAMVVAVEEFVTTHPDHKGSIGFLITSDEEGQATDGTVVVCNQLKARGEQLDYCLI
                     GEPTSTRQLGDMIKNGRRGSMSGKLTVKGIQGHIAYPQLAQNPIHLA"
     gene            complement(638..1000)
                     /locus_tag="VFA14_02805"
     CDS             complement(638..1000)
                     /locus_tag="VFA14_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HZS80343.1"
                     /translation="MNTQVTLYGIPNCSTVKKARAWLDANGIGYTFHDFKKAGLDAGL
                     IKTWLEDIPWDTLLNRKGTTWRSLSDVRKASIIDSESAGRLMLELPSIVKRPVLQTSG
                     RPAQAGFSETQYQQIFKN"
     gene            complement(1106..1942)
                     /gene="dapD"
                     /locus_tag="VFA14_02810"
     CDS             complement(1106..1942)
                     /gene="dapD"
                     /locus_tag="VFA14_02810"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HZS80344.1"
                     /translation="MTLSIQHIIDQAWEDRASLSSKTAPAGIRNAVAEVIAGLDAGTL
                     RVADRQGVGQWQVNQWIKKAILLSFRLEDNVPVSAGVPGYPQFYDKVPTKFASYTAED
                     FARGGFRVVPPAVARHGSFIGRNVVLMPSYVNIGAYIDEGAMVDTWATVGSAAQIGRN
                     VHLSGGVGIGGVLEPVQAGPVIIEDNCFIGARSEVVEGVIVEENSVLSMGVYIGQSTR
                     IYDRETGAVHYGRVPAGSVVVPGNLPSKDGKYSLYAAIIVKKVDAQTRSKTSINELLR
                     GA"
     gene            complement(1999..>2373)
                     /locus_tag="VFA14_02815"
     CDS             complement(1999..>2373)
                     /locus_tag="VFA14_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZS80345.1"
                     /translation="AIQSASIAAWNDETHVQENRASYLAKFSLVTPLLQEVLDVSLPD
                     AGFYLWARVDKLAAISDTEYARRLYAEYNVTVLPGSYLARKANGIDPGRQRIRMALVA
                     EVEACLEAAQRIVAFTRQLAGS"
BASE COUNT          463 a          692 c          716 g          502 t
ORIGIN      
        1 cggccagatg aatgggattt tgcgccagtt gtggataggc gatgtggccc tgaatgcctt
       61 tgacggtcag tttgccggac atggagccgc gacggccatt cttgatcatg tcgccgagtt
      121 gcctggtcga tgtcggctcg ccgatgaggc aatagtcgag ctgctcgccg cgcgccttca
      181 gttgattaca gacgaccacg gtaccgtcgg tggcctggcc ttcttcatcg ctggtgatca
      241 aaaagccgat cgaacctttg tggtcgggat gcgttgtgac gaattcctcc accgcgacga
      301 ccatggctgc aatcgaggtt ttcatgtccg cggcgccgcg accaaacagt ttgccattgc
      361 gctgggtcgg cacgaacggc ggcgattgcc actcgctggc cgggccggtc ggcaccacgt
      421 ctgtatggcc ggcaaaggtc agcagcggtt gcgcctgacc tttgcgcgcc cacaggttgg
      481 tgacagcgcc ggactggatg gtttcgcaga cgaagcccag cggcgtcagc agctccgcca
      541 gacgcgcctg acagcctttg tcttccggcg tcacggaaga tagcgcgatc agttgctggg
      601 ccagaacaag ggttttactc atggcgaatg cgggaaatta gttcttgaaa atctgttgat
      661 attgcgtctc gctgaaaccg gcctgcgcgg gccggccgct ggtttgcagc accggacgct
      721 tgacgatgga cggcagttcg agcattagcc tgccggcgct ttcgctatcg atgatgctcg
      781 ccttgcgcac gtcggacagg ctgcgccagg tggtgccttt gcgattgagc agcgtatccc
      841 aggggatatc ctctagccaa gtcttgatca ggccggcgtc gagtcccgct ttcttgaagt
      901 catggaaggt gtagccaata ccgttggcat cgagccaggc gcgcgccttc ttgaccgtac
      961 tacagttggg aatgccgtag agagtgacct gtgtgttcat gaaatccata atcctgttct
     1021 gcaaaaaaat caaggccggc gtcttgccat gccggcctcg ctgcaaaaaa acggccggca
     1081 atacgccggt cattcatggc gccggtcagg cgccgcgcaa caattcattg atcgacgtct
     1141 tggagcgggt ttgcgcgtcc accttcttga cgatgattgc tgcatacagg ctgtacttgc
     1201 catccttcga cggcaagtta cccggcacca caacagaacc ggccggcacg cggccgtaat
     1261 gcactgcacc ggtttcacgg tcataaatcc tggtcgactg gccgatgtag acgcccatcg
     1321 acagcactga gttttcttcg acgatcacgc cttcgacgac ttccgagcgc gcgccgatga
     1381 agcagttgtc ttcgatgatg accggaccgg cctgcaccgg ctccagcacg ccgccgatgc
     1441 cgacgccgcc agacaggtgg acattcctgc caatttgcgc ggcagagccg acggtggccc
     1501 aggtatcgac catcgcacct tcatcgatat aagcgccgat gttgacatag gagggcatca
     1561 gtacgacgtt cctgccgatg aaagagccat gacgagccac cgccggcggc acgacgcgga
     1621 agccgcccct ggcgaaatct tctgcagtgt agctggcgaa cttggtcgga accttgtcat
     1681 agaactgcgg gtagcccggc acgccggcac tgaccggcac attgtcttcc aggcgaaacg
     1741 acaacaggat ggctttcttg atccactggt tgacttgcca ttgaccgacg ccttgacgat
     1801 cggccacgcg cagcgtgcct gcgtcgaggc cggcgatgac ttcggccacg gcgttgcgga
     1861 taccggcagg agcggtcttc gacgacaggc tggcgcggtc ttcccacgct tggtcgatga
     1921 tgtgctggat ggatagtgtc atcttgtctt gtttaggttg aaaatgaatc aaaatcgggc
     1981 gacggatgcc ttgacgaatc aggagccggc cagctgacga gtgaaagcga cgatacgctg
     2041 cgctgcttcc agacatgctt ccacttcggc gaccagcgcc atgcggatgc gttgccggcc
     2101 cggatcgatg ccgttggctt tgcgcgccag gtaactgccc ggcaataccg tgacattata
     2161 ttcggcatag agacgcctgg catattccgt atcgctgatg gcggccaact tatcgaccct
     2221 ggcccataga taaaagccgg cgtccggtaa agaaacatcc agcacttctt gcagcagcgg
     2281 cgtgaccagg ctgaatttgg cgaggtagct ggcacggttt tcctgcacgt gggtttcatc
     2341 attccaggct gcaatcgagg cactctggat cgc
//
LOCUS       DASRDH010000123         5255 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_896011,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000123 DASRDH010000000
VERSION     DASRDH010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 5255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5255
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_896011"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="VFA14_02820"
     CDS             complement(<1..284)
                     /locus_tag="VFA14_02820"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013236090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZS80346.1"
                     /translation="MSLPSSDVSAKLSAPINLPLVRRLFSTPACVAESDFLRREVASR
                     MLERLLLVKIAPQRMLDAGCGEGADIVSLQQRFADARIIGLDGAFNMLLLA"
     gene            359..1108
                     /locus_tag="VFA14_02825"
     CDS             359..1108
                     /locus_tag="VFA14_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007879562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HZS80347.1"
                     /translation="MFARLFNTSLSLLRQFQHLPQLLPSSCVLCAQTGRNALCQPCRQ
                     HYFTTRPRRCMQCAMPIPTTAKEIRCGDCLKHRPAFDATIVAADYATPADQLVLALKF
                     GAKLPLAPLMADMLRDAIRHDTERGLAMPTLLTAVPLSTSRLQERGFNQSLEIARTLS
                     RRLDTALAPHLLVRLRDTQAQATLPTKERRHNVHRAFAVPPAAIDHICGCHVGVIDDV
                     ITTGATLNEVAATLKRFGAQRVTNIVFARTV"
     gene            1131..1613
                     /gene="trmL"
                     /locus_tag="VFA14_02830"
     CDS             1131..1613
                     /gene="trmL"
                     /locus_tag="VFA14_02830"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006461509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HZS80348.1"
                     /translation="MFHVVLVEPEIPPNTGNIIRLCANTGTQLHLIEPLGFPLDDARM
                     RRAGLDYHDYATMQVHPHWECFIEKRRADPQYDAARMFALTTHGSTPFASVAFRPGDT
                     FVFGSETRGLAPELLTSFSASQRIRLPMRPNNRSLNLSNTVAVVVYEAWRQNGFSGGS
                     "
     gene            2758..3291
                     /locus_tag="VFA14_02835"
     CDS             2758..3291
                     /locus_tag="VFA14_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008332645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HZS80349.1"
                     /translation="MSSRFRSLASWIAIIHLAFCSLLPTEVLATPHSESAGVDHDYLL
                     GNLQISHPWTRATVPGQSSGGVYLRIENKGPTSDRLVSASSPIAQITEIHTMSMEDNV
                     MKMREIGSIEIKPAEEIFMQPGKGYHIMLIGLNQSLKAGDRILLTLTFEKAGKLDVMA
                     YVKGKSVDKDSMEHMRQ"
     gene            complement(3305..4753)
                     /gene="sbcB"
                     /locus_tag="VFA14_02840"
     CDS             complement(3305..4753)
                     /gene="sbcB"
                     /locus_tag="VFA14_02840"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016836605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HZS80350.1"
                     /translation="MSNHSFLWHDYETFGAQVRRDRPAQFAAIRTDAELKEIGDPIML
                     YCRPAPDFAPDPQSCLITGITPQICLERGIPEYQFAACIEQAMSEPGTISVGYNTIRF
                     DDEITRFMFWRNLIDPYAREWQNHCGRWDLLDVVRATYALRPDGITWPVKEDGVTPSF
                     MLEHLTMANGLLHDAAHDALSDVRATIALARLIRDRQPRLFDFCLALRKKERVADEIP
                     VAKPFLHVSGMFPAAQGCLALMWPLGPHPTNRNEIIAWDLSRDPGELAGLDAGTIRTR
                     MFCKTDDLPEGISRLPIKTIHLNKSPVVIANLKTLSAGRAAELGIDLDAALQHAASAA
                     ALPKELGAVWDSVFQRSKEAAPDVDEDLYGSFIGNNDRRRLTQLRALSPQKLAQARIS
                     FDDIRLEALLFRYRARNFPESLSAEDRLRWEEHRAARLLDGAGGARTLDALFGQIDQL
                     PENAGERREEIFRALRDYARMIAPERHWEAIN"
     gene            complement(4845..>5255)
                     /locus_tag="VFA14_02845"
     CDS             complement(4845..>5255)
                     /locus_tag="VFA14_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1993 domain-containing protein"
                     /protein_id="HZS80351.1"
                     /translation="RHIEADALLEARLFPDMFHLIRQVQIAADFAKGVSARLAGVEAP
                     SHDDNEANFADLQVRLDKTLAFIATLKPEQFVGAETRDIVLRPGTPKEKRFTGHDYLL
                     HYGLQHFFFHITTAYNILRHNGVEIGKADYIGTY"
BASE COUNT         1225 a         1551 c         1385 g         1094 t
ORIGIN      
        1 gccagaagca acatattgaa ggcaccatcc agaccgatga tccgagcatc ggcaaagcgc
       61 tgctgcaaag agacgatgtc ggccccctcg ccgcaaccag catctagcat acgctgcggc
      121 gcgatcttga ccagcagtag gcgctccagc atgcgcgatg ccacttcgcg tcgcagaaaa
      181 tccgattcag ccacgcacgc cggtgtggaa aacagacggc gcaccagcgg cagattgatc
      241 ggcgcactga gtttggcgct gacgtcagac gagggcaaag acatggggag agccggcaaa
      301 cgaaatgaga taatcggcgc tagtgtagtc catttctcag ccatcaggat gttccgacat
      361 gtttgcgcgc ctgttcaata cgagtctgag tttgctgcgg cagtttcaac acttgccgca
      421 actgctgcca tcttcatgcg tgctatgcgc gcaaaccggc cggaacgcac tatgccagcc
      481 ctgccggcag cattatttca cgacacgtcc acgccgctgc atgcagtgcg ccatgccgat
      541 tccgaccacg gccaaggaaa tccgctgcgg cgattgtctg aaacaccgac ccgctttcga
      601 tgccaccatc gtcgccgccg attacgccac gcctgcagac cagctggtac tggcgctgaa
      661 gttcggcgcc aagctgccgc tcgcgccgct catggcagac atgctgcgcg acgccattcg
      721 ccatgacaca gagcgtggcc tggcaatgcc gactttgttg acggcggtgc cgctgagtac
      781 atcgaggcta caggaacgcg gcttcaatca gtcgctggaa atcgcccgga cgctgtcgcg
      841 tcggctcgac acggcactcg ctccgcatct tctggtcagg ctacgcgaca cccaagccca
      901 agcgacgttg cctaccaagg agcgtcggca caacgtacac cgtgcttttg cagttccgcc
      961 tgcggcgata gaccacatat gcggttgtca tgtcggtgtg attgatgatg tcatcactac
     1021 cggcgccacg ctcaacgaag ttgctgccac gctcaaacgc tttggcgcgc agcgcgtgac
     1081 gaacatcgta tttgcccgca cggtatgaaa gtaaacacaa ggagactcag ttgtttcacg
     1141 tcgttctggt cgagccggag attcctccca ataccggcaa catcattcgc ctgtgcgcta
     1201 acaccggcac acagcttcat ctaatcgaac cgctcggttt tccactcgac gacgccagaa
     1261 tgcgccgcgc cgggctggac tatcacgact acgcgaccat gcaggtccat ccgcactggg
     1321 agtgcttcat cgaaaaacgc cgcgccgatc cgcagtatga tgcggcccgc atgttcgcgc
     1381 taaccacgca tggctcgaca ccatttgcca gcgttgcttt ccgccctggc gacacctttg
     1441 tgttcggctc cgaaacacgc ggcctcgcgc ctgaattgct cacgtcgttt tcggcatcgc
     1501 aacgtattcg cctgccgatg cgaccgaata atcgcagcct gaatttatcc aacacggttg
     1561 ccgtagttgt gtatgaagcc tggcgacaga acggatttag cggcggcagt taaaaaatag
     1621 cgcgcgcagc gaccagactc tcatgatgac tcgtgcgtca cgcacggcgt agtatgcctg
     1681 cgccgtgctg aaagacgaag gggctatagt acggcagctg ctgaggcagc aacgtcagcc
     1741 gtgtccctat cacaaactgt aacggtccag tgaaggaacc catagtccga gcctgaaata
     1801 taatccctcg tgctgataac aagctcccgg caagccgcac cgctaacaaa ctctaattca
     1861 ctgatactac tcagttccct cataatttcc tgactgcctg ttccaggtaa agtgttgcct
     1921 agaaattacc tgattgcctg ttccgtcatc acaaacttta acgaccgagt aactagatcc
     1981 atatagcgag cctggaatat aatccatcgt gctgataaca aactcccggc aagccgcacc
     2041 gctaacaagc tccaattcac tgatactcag ttctctcatg atcaatcctt cctagtttgg
     2101 gtaaagcgtc gctcagaaat ttcctgactg cctgttccat cattcccccg caacgcaaaa
     2161 cggggatgat gacttgtgcg tcgcgcacgg cgtagcatgc ttgcgccgtg ctgaaaaacg
     2221 aggtaggggg ttacagtacg gcagctgctg aggcagcaat gccctcatca caaactttaa
     2281 caacccaatg aatagatcca tagtccgagc ctggaacata atccatcgag ctaataacca
     2341 actcccggca agccgcaccg ctaacaagtt ccaattcact gatactcagt tctctcatga
     2401 tcaatccttc ctaatttgta aggtacagcg tcgctcagaa atttcctgac tgcctgttcc
     2461 accattcccc ggcaacgcaa aacgggggta atgatttgtt actccagaac aagtcttgac
     2521 agagtagacc cccctctcct ttgcgtatat aggagaattc tgaaactaga ataaaagaaa
     2581 tctgaaactg aaaaggtcca gtcattctgg gcactgcgtt aaattattct tgactgaccg
     2641 aactcgcaac gaattaacca gtcctctatt tctgattggt cccaaaagac gaggggctac
     2701 agtgatgcgc cagccacacg cgcgttcaaa aatactgttt gctataatct tcgccttatg
     2761 tcatcacgct ttcgctccct cgcctcctgg atcgccatta tccatctggc gttctgcagt
     2821 ctgttaccga ccgaggtact ggccaccccc cattccgaaa gcgccggcgt cgaccatgac
     2881 tacctactcg gcaacctgca aatcagccat ccctggactc gcgccaccgt tcccggccaa
     2941 tcttccggcg gcgtctatct gcgtatcgaa aataaaggtc caaccagcga cagactggtt
     3001 tctgcatctt cgcccattgc ccaaatcacc gaaattcaca ccatgtccat ggaagacaac
     3061 gtgatgaaga tgcgtgaaat cggcagcatc gagatcaaac ccgccgaaga aatcttcatg
     3121 caaccgggca agggctacca cattatgctg atcggcctga accaatcatt gaaagccggc
     3181 gacaggattt tattgacgct gaccttcgaa aaagctggca aacttgatgt catggcctac
     3241 gtcaagggca aaagcgtgga taaagacagc atggaacata tgcgtcaatg aagcagacag
     3301 caaactagtt aatagcttcc caatgccgct ccggagcaat catcctggcg tagtcgcgca
     3361 gcgccctgaa gatttcttct ctgcgctcac cggcattttc cgggagctgg tcgatttgcc
     3421 caaataatgc atccagcgtg cgcgcgccgc ctgcgccatc aagcaggcgt gcggcgcgat
     3481 gttcttccca acgcaatctg tcctccgctg acaaactctc ggggaagttg cgcgcgcgat
     3541 agcggaacag caatgcctcc agcctgatgt cgtcgaaact gatgcgcgct tgcgccagtt
     3601 tctgcggcga cagcgcgcgc aattgcgtca gccggcggcg atcattgtta ccgatgaagc
     3661 tgccatacaa atcctcatcg acatccggcg ctgcttcttt ggagcgctgg aacacgctgt
     3721 cccagacagc gcccagttct ttcggcaacg cggcggcgct tgccgcatgc tgcaaggcgg
     3781 catcaagatc gatgccaagt tcggctgcac gcccggccga taaggttttc aggttggcga
     3841 tgacgaccgg cgacttgttc agatgaatgg ttttgatcgg cagccgcgag atgccttccg
     3901 gtaaatcatc ggttttacag aacatgcgcg tgcggatcgt cccggcatcc agtccggcca
     3961 gttcgcccgg atcgcgactc agatcccagg caatgatttc atttctgttg gtcgggtgag
     4021 ggccaagcgg ccacatcagc gccagacaac cctgcgccgc aggaaacatg ccggagacgt
     4081 gcaaaaaagg tttcgccacc gggatttcgt cggcgacacg ctcctttttg cgcagggcca
     4141 ggcaaaagtc gaacaggcgc ggctgccggt cgcgaatcag tcgggcaagg gcgatcgtgg
     4201 cgcgcacgtc ggacaaggca tcgtgcgcgg catcgtgcag caagccgttg gccatggtca
     4261 agtgttccag catgaaactg ggcgtgaccc cgtcttcctt taccggccat gtgatgccat
     4321 cggggcgcag cgcataggta gcgcgcacaa cgtccagcaa atcccagcgt ccacaatggt
     4381 tttgccattc gcgcgcatac ggatcgatca gattgcgcca gaacatgaaa cgcgtgatct
     4441 catcatcgaa gcgaatggtg ttgtaaccga cgctgatggt gccgggttcc gacatggcct
     4501 gttcgatgca ggcggcgaac tggtattcag ggatgccgcg ctccaggcag atttgcgggg
     4561 taataccggt gatcaggcag gattgcggat cgggcgcgaa gtccggcgcc ggtcggcaat
     4621 agagcatgat cgggtcgccg atttccttga gttcagcatc ggtgcggatc gccgcaaact
     4681 gcgctggtcg gtcacgacgc acctgagcgc cgaaggtttc gtagtcgtgc cagagaaagg
     4741 agtgattgga catcgagagt agactgaagc aaaaactcac ttgcggaaaa gagaggagta
     4801 ggcaacacct tgtcggtaca agtctcggtc cgtcacccac ccgatcagta agtaccgatg
     4861 taatcggcct tgccgatttc cacgccgtta tggcgcagaa tgttataggc cgtggtgatg
     4921 tggaaaaaaa aatgctgcaa accgtaatgc agcagatagt cgtggccggt aaaacgtttt
     4981 tccttcggcg taccggggcg cagcacgata tcgcgcgttt cggcgccgac gaactgctcc
     5041 ggtttcagcg tggcgataaa ggccagggtc ttgtccagtc gcacttgaag atcggcgaag
     5101 ttggcttcgt tgtcatcatg ggacggagct tccacaccag ccagacgcgc tgatacgcct
     5161 ttggcaaaat cagccgcgat ctgtacctgg cgaatcagat gaaacatgtc tggaaacaga
     5221 cgcgcttcca gcaacgcatc ggcttcgata tgcct
//
LOCUS       DASRDH010000124         4383 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_258553,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000124 DASRDH010000000
VERSION     DASRDH010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 4383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4383
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258553"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..703)
                     /locus_tag="VFA14_02850"
     CDS             complement(<1..703)
                     /locus_tag="VFA14_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZS80352.1"
                     /translation="MFSAAPASLLSQRRVYLSGLLIAIIGAIFFSAKAIVAKLMYRYH
                     VDAVMVISLRMMFSFPLFAVVAIWQARHAPPLAPRDCLRIVLLGLVGYYLSSFLDFLG
                     LQYISAGLERLILFLTPSFVLLISFAFYQRRVSMMEWGALLLSYLGTALVLQYDANAG
                     GSNVALGSTLVLGAAITYALYLVFSGELVRRVGAMRLVAYAMCVSSVACIVQFFILRP
                     ASALALPAEVYQLSLI"
     gene            886..1611
                     /locus_tag="VFA14_02855"
     CDS             886..1611
                     /locus_tag="VFA14_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HZS80353.1"
                     /translation="MAGHSKWANIQHRKGRQDEKRGRIWTRLIKEITVAARMGGGEPD
                     ANPRLRLAIDKAADANMPKDNVTRAIQRGVGALDGINYEEVRYEGYGINGAAIIVDCM
                     TDNRIRTVAEIRHVFSRFGGNMGAEGSVSFMFRHCGQLLYAPGLSEDTVMEVALEAGA
                     DDVIGDAEGGIEVLTPPHHFATVKAAMEEAGFQAEMAEIIMRPATETVFTGDDAIKMQ
                     KLLDALESLDDVQEIYSNASIEE"
     gene            1654..2931
                     /gene="purD"
                     /locus_tag="VFA14_02860"
     CDS             1654..2931
                     /gene="purD"
                     /locus_tag="VFA14_02860"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006464673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HZS80354.1"
                     /translation="MKILIVGSGGREHALAWKIAQSPRIQTVYVAPGNGGTALDSRLQ
                     NIAITDLDELADFVEKEHVALTVVGPEIPLAAGIVNLFRSRGLKIFGPTKEAAQLESS
                     KDFSKSFMKRHAIPTAEYQTFSDVEAAHAYIARKGAPIVIKADGLAAGKGVVVAMTLE
                     EAYSAVDMMLSDNRLGDAGARVVIEEFLAGEEASFIVMVDGKNILPLATSQDHKRLQD
                     NDQGPNTGGMGAYSPAPIVTPALHARVMRDIIVPTVQGMAKDGIPFSGFLYAGLMIDA
                     QGNPKTLEFNCRMGDPETQAIMVRLKTSLLTVMEHAIDGTLDAIELEWDQRTALGVVM
                     AAAGYPDTPRKGDPISGIPAETADCVIFHAGTTLRDGVSGDRLTTSGGRVLCVVGLGD
                     NVKMAQKHAYAAVDLVHFEGAQLRRDIGWRATH"
     gene            2982..3806
                     /locus_tag="VFA14_02865"
     CDS             2982..3806
                     /locus_tag="VFA14_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008116937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HZS80355.1"
                     /translation="MPQHLLNLDALINIYLLPFGLKVLAALVIWFAGGMLVSTFARVA
                     RGVLTARRIDATLVNYAVSAIYVILRILLVMGILEVCGVPTTSFAAMIGAVGVAMGVA
                     WSGLLSNFAAGIFLMVLRPFKVGNTITAAGQTGTVTDIGLVTTTLLTDNNLRVIIGNN
                     KLFSDTITNYSTHPTRRVDLRCQIAYGVEPAQAIARLTDKVSRIPNVLTDPAISVSIL
                     EFNTAGTLLAVRLYAPTSNYGQVYNDTNHAIAQTCAEAGWPAPATYQVSLPPAPSV"
     gene            3822..>4383
                     /locus_tag="VFA14_02870"
     CDS             3822..>4383
                     /locus_tag="VFA14_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HZS80356.1"
                     /translation="MTVTPSANPPATVKAYLQDLQHRIVAALESVDGKLFLTDAWERP
                     EGGGGVSRVVEEGNVLERGGVGFSHVMGKNLPPSAAANRPETAGRAWEAMGVSLVLHP
                     RNPYAPTVHMNVRFFSAKAQGQEPVWWFGGGMDLTPYYGFAEDAAHFHRTCRAALAPF
                     GADLYPRFKKWCDDYFYLKHRQEARGV"
BASE COUNT          997 a         1386 c         1235 g          765 t
ORIGIN      
        1 tgatcagcga caattgatat acctcggccg gcaatgccag cgccgatgcc ggacgcagga
       61 taaaaaactg gacgatacat gccaccgatg acacacacat ggcgtaggcc accagccgca
      121 tcgcaccgac gcgacgcacc agctcgccag aaaaaaccag atacaaggca taagtaatcg
      181 ccgcgcccag caccagagta ctgcccagtg ccacattgct gccaccggca ttggcgtcat
      241 actgcaacac cagcgccgta ccaagatagg acagcaacaa cgcgccccat tccatcatgc
      301 tgaccctgcg ctgatagaac gcgaaagaaa tcagcagcac gaatgatggc gtcagaaata
      361 gaatcagccg ctccagcccg gccgagatgt attgcaggcc gaggaaatca aggaaactcg
      421 acaggtaata accgaccagg ccgagcagaa cgatcctgag gcagtcgcgc ggcgctagcg
      481 gcggcgcatg acgcgcttgc caaatcgcca cgacggcaaa tagcggaaat gaaaacatca
      541 tgcgcaggct gatcaccatg accgcatcga cgtgatagcg atacatcagc ttggcgacga
      601 tagccttggc ggaaaagaaa atggcgccaa tgatggcaat cagtaaacca ctgagataaa
      661 cacgacgctg ggaaagcagg gaagcaggcg ctgcagaaaa catcccgtaa ttgtaaacac
      721 ttcgcgctgc gtctgccggg actagtattt ctacggaaag atgggcggag ggcgacggaa
      781 acggacgata tgataaaatt ttgggctgat tataatcggg atctctttct cctgccgcgg
      841 tccagcggat gaaagtgcgt tccatcgagg caggaaaaat tcatcatggc tggacacagc
      901 aaatgggcca acattcagca ccgtaagggc cgtcaggacg aaaagcgcgg cagaatctgg
      961 acgcgtctga tcaaggaaat caccgttgca gcccgcatgg gcggcggcga accggatgcc
     1021 aacccgcgtc tgcgcctggc catcgacaag gccgccgacg ctaatatgcc caaagataac
     1081 gtcacccgcg ccatccagcg cggcgtcggc gcactcgacg gcatcaacta cgaagaagtc
     1141 cgctacgaag gctacggcat caacggcgcc gccatcatcg tcgactgcat gaccgacaac
     1201 cgcatccgca ccgtggccga aatacgccac gtgttttcca gatttggcgg caacatgggt
     1261 gctgaaggtt cggtgtcgtt catgttcagg cattgcggcc agttgctgta cgcgccaggc
     1321 ctaagtgagg acactgtcat ggaagtcgcg ctcgaagccg gcgccgatga cgtcatcggc
     1381 gacgcggaag gcggcatcga agtcctgacg ccgccgcacc acttcgctac tgtcaaggcc
     1441 gcgatggaag aagccggctt ccaggccgaa atggctgaaa tcatcatgcg gcccgccacc
     1501 gaaaccgttt tcactggcga cgacgccatc aagatgcaaa agctgctcga cgctctggaa
     1561 agtctcgatg atgtgcagga aatctacagc aacgcctcga tcgaagagta aaccgtaagt
     1621 cagctgacca accccattcc atttctccct ctcatgaaaa ttctcattgt cggctccggt
     1681 ggccgtgaac acgcgctagc ctggaaaatc gcccaatcgc cccgcatcca gaccgtctac
     1741 gtcgcccccg gcaacggcgg caccgcgctc gacagccgcc tgcaaaatat tgccatcacc
     1801 gatctcgacg aactcgccga cttcgtcgaa aaagaacatg tcgcgctgac cgtggtcggc
     1861 ccggaaatcc cactggccgc cggcatcgtc aatctgttcc gctcgcgcgg cctgaaaatc
     1921 ttcggcccga ccaaagaggc cgcacaactg gaaagttcga aggatttctc aaaatctttc
     1981 atgaaacgcc atgccatccc gactgccgag taccagacct tctccgacgt cgaggctgca
     2041 cacgcttata tcgcgcgcaa aggcgcgccc atcgtcatca aggcggatgg tctggccgcc
     2101 ggcaagggcg tagttgtagc aatgacgctg gaagaagcat acagcgccgt agacatgatg
     2161 ctgtcggata acaggctcgg cgacgccggc gcgcgcgtgg tgatcgaaga attcctggcc
     2221 ggcgaagaag ccagcttcat cgtcatggtc gacggcaaaa acatcctgcc gctggccacc
     2281 agccaggacc acaagcgcct gcaggacaac gatcagggtc cgaacaccgg cggcatgggc
     2341 gcctactcac cggccccaat cgtgactcct gcgctgcatg cccgcgtgat gcgcgacatc
     2401 atcgtgccga ccgtgcaagg catggccaag gacggcatcc cgttctccgg cttcctgtat
     2461 gcaggcctga tgatcgacgc ccagggcaat ccgaaaacgt tggaattcaa ctgccgcatg
     2521 ggcgacccgg aaacgcaggc aatcatggta cgtctcaaga ccagcctgct gactgtgatg
     2581 gaacatgcaa tcgatggtac gctcgacgcc attgaactgg aatgggacca gcgcaccgcg
     2641 ctgggcgtgg tgatggccgc tgccgggtat ccggatacgc cgcgcaaggg cgacccgatc
     2701 agcggcattc cagccgaaac cgccgattgc gtgattttcc acgccggcac gacactccgc
     2761 gacggtgtca gcggcgacag gctgaccacc tccggcggac gtgtattgtg cgtggttggt
     2821 ctgggcgaca acgtcaagat ggcgcagaaa cacgcctatg ctgccgtaga cctggtccat
     2881 ttcgaaggcg cacaactgcg ccgcgatatt ggctggcgcg cgacacacta aaccccgttt
     2941 ttgcgtagaa tcgcaaaagg cccgccacga caggagaaaa catgccgcag catctgctca
     3001 atctcgacgc cctgatcaac atctatctac taccattcgg tctcaaggtc ctggcagcgc
     3061 tggtgatctg gttcgccggc ggcatgctgg tcagcacctt tgccagggtg gcgcggggcg
     3121 tgctaaccgc gcgtcgtatc gatgcgacgc tggtgaacta tgccgtttcg gcgatatacg
     3181 tcatcctgcg catattgctg gtgatgggca tcctcgaagt ctgcggcgtg ccaaccactt
     3241 cattcgccgc catgatcggc gcggtcggcg tagcgatggg cgtggcctgg tccggcctgc
     3301 tgtcaaactt cgcggccggc atcttcctga tggtgctgcg ccccttcaag gtcggcaaca
     3361 ccatcacggc ggcaggtcag accggcaccg tgaccgatat cggcctggtc accaccaccc
     3421 tgctcaccga caacaatctg cgcgtcatca tcggcaacaa caagttgttc tccgacacca
     3481 tcaccaatta cagcacgcac ccgacccgcc gcgtcgatct gcgctgccaa atcgcctacg
     3541 gtgtcgaacc ggcgcaggcg atcgcacgcc ttaccgacaa ggtctcgcgc attcctaacg
     3601 tgctgactga tccggccatt tcggtgtcca tcctcgaatt caacaccgcc ggcaccttgc
     3661 tggcggtgcg cctgtatgca ccgacgtcga attacggcca ggtctacaac gacaccaatc
     3721 acgccattgc gcaaacctgc gccgaagcag gctggcccgc gccggcgacg tatcaggtca
     3781 gcctcccgcc cgcccccagc gtctgaatca ctctgctgcc aatgaccgta actccatccg
     3841 ccaatcctcc cgctaccgtc aaagcctatc tgcaagacct ccagcaccgc atcgtcgcgg
     3901 cgctggagag tgtcgatggc aagctttttc tgaccgatgc ctgggaacgt cccgaaggcg
     3961 gcggcggtgt ctcacgcgtg gtcgaagaag gcaatgtgct ggaacgcggc ggcgtcggtt
     4021 tttcacatgt catgggcaaa aacctgccgc cttcggctgc cgccaaccgc ccggaaaccg
     4081 ccggccgtgc atgggaagcc atgggcgtgt cgctggtgct acatccgcgc aatccgtatg
     4141 ccccgacggt gcatatgaac gtccgttttt tcagcgccaa ggcacagggc caggagccgg
     4201 tctggtggtt cggcggcggc atggatctaa ccccgtatta cggctttgcg gaagacgccg
     4261 cgcatttcca ccgtacttgc cgcgctgcat tggcgccgtt cggcgccgat ctgtatccgc
     4321 gcttcaagaa gtggtgcgac gactattttt atctgaaaca tcgccaggaa gcgcgcggcg
     4381 tcg
//
LOCUS       DASRDH010000125         3551 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1414404,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000125 DASRDH010000000
VERSION     DASRDH010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 3551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3551
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1414404"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1212
                     /locus_tag="VFA14_02875"
     CDS             <1..1212
                     /locus_tag="VFA14_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011979365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /protein_id="HZS80357.1"
                     /translation="VAKDEKVRTDRLSANGLVTKPPARYSEATLLSAMEGAGKLIDSD
                     ELREAMVGKGLGTPATRAAIIEGLLNEKYLIREGREIMPTAKAFQLMTLLQGLGVDEL
                     TEPELTGEWEYKLAQMERGKISRDEFMREIAQMTQIIVKRAKEYDNDTIPGDYVTLHT
                     PCPNCGGVVKENYRRFACTQCEFSISKVPGGRQFEIPEVEELLLNRTIGPLQGFRSKM
                     GRPFAAILKVSRDEEINNYKLEFDFGQNEGESEGREVVDFSTQAPLGPCPKCGSGVYE
                     MGLAYVCEKTVAQPKACDFRSSRIILQQEILPDQMVKLLNEGKTDLLPGFISQRTKRP
                     FKAFLVRGKDGKNSFEFEERKAKPPARGEATVGAGDDAEASARPAVTAKKPAARKPAV
                     KKTASRKAAAA"
     gene            1428..1691
                     /gene="infA"
                     /locus_tag="VFA14_02880"
     CDS             1428..1691
                     /gene="infA"
                     /locus_tag="VFA14_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007881321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HZS80358.1"
                     /translation="MAKEELLEMNGLVEEVLPDSRFRVHLENGHQLIAYTSGKMRQNH
                     IRILAGDKVTLELSPYDLSKGRIIFRHLEKRGPVGGNQQRRRY"
     gene            1828..2424
                     /gene="hisB"
                     /locus_tag="VFA14_02885"
     CDS             1828..2424
                     /gene="hisB"
                     /locus_tag="VFA14_02885"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HZS80359.1"
                     /translation="MFTPRTAEVVRNTNETQIRVALNLDGVGRQKLDTGVPFLDHMLD
                     QVARHGLIDLDIHARGDLHIDAHHTVEDVGITLGQAFAKAIGDKKGICRYGHAYVPLD
                     EALSRVVIDFSGRPGLEYHIPFARAMIGSFDVDLTSEFFHGFVNHAQVTLHIDNLRGA
                     NAHHQCETVFKAFGRALRMAIELDPRAAGSIPSTKGSL"
     gene            2478..3116
                     /gene="hisH"
                     /locus_tag="VFA14_02890"
     CDS             2478..3116
                     /gene="hisH"
                     /locus_tag="VFA14_02890"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HZS80360.1"
                     /translation="MNKIVVVDYGMGNLRSVAQALCHVAPEADVCISGVVADIRSADR
                     IVLPGQGAMPDCMRCLRESGLQEAVVAATHSKPLLGVCVGEQMLFDWSEEGDTPGLGL
                     LPGKVVRFRFDGMVQEDGSRFKVPQTGWNRVHQRAAHPLWDGIGEQAYFYFVHSYYAQ
                     PGESAHIVGETVYGQAFACAVASGNIFATQFHPEKSAAAGLQLYRNFVHWKP"
     gene            3190..>3551
                     /locus_tag="VFA14_02895"
     CDS             3190..>3551
                     /locus_tag="VFA14_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008332447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HZS80361.1"
                     /translation="MLLIPAIDLKDGHCVRLKQGDMDQATVFSENPADMAQHWLLQGA
                     RRLHLVDLNGAFAGKPKNEPAVKAILRAVRDFAADNHGEEIPVQLGGGIRDLDTIERY
                     LDDGLSYIIIGTAAVKNPG"
BASE COUNT          764 a          984 c         1029 g          774 t
ORIGIN      
        1 gtcgctaagg atgaaaaggt caggaccgac aggctgagcg ccaatggtct ggtcaccaaa
       61 ccgcctgcgc gctacagcga agcgacgctg ttgtcagcca tggaaggcgc cggcaagctg
      121 atcgattccg acgagctgcg cgaggcgatg gtcggcaagg gcctgggtac accggccacg
      181 cgtgcggcca tcatcgaagg tttgctcaac gaaaaatacc tgatccgtga gggccgcgaa
      241 atcatgccga cggccaaagc ctttcagctc atgaccttgc tgcaagggct gggcgtggat
      301 gaactgaccg agccggaact gaccggcgaa tgggaatata aactggcgca gatggagcgt
      361 ggcaagatca gccgcgacga gttcatgcgc gaaatcgcgc agatgacgca gatcatcgtc
      421 aagcgcgcca aggagtacga caatgacacc atccccggcg actatgtcac cttgcatacg
      481 ccctgtccga attgcggcgg tgtggtaaag gaaaactatc gtcgtttcgc ctgtacccag
      541 tgcgaattct caatcagcaa ggtgcccggc ggccggcagt tcgaaatccc tgaggtggaa
      601 gaattgctgc tcaatcgcac cattggccca ctgcaaggtt ttcgctccaa aatgggccgt
      661 ccgttcgccg caatcctgaa ggtttcgcgc gatgaggaaa tcaacaacta caagctcgaa
      721 ttcgacttcg gccagaacga gggtgaaagc gaggggagag aagttgtcga tttcagtacg
      781 caggcgccgc tcggcccctg cccgaaatgc ggcagcggcg tgtacgaaat ggggctggcc
      841 tatgtctgtg aaaagacggt tgcccagccc aaggcttgcg atttccgcag cagccgcatc
      901 attttgcagc aggaaatcct gccggaccag atggtcaaac tgctcaacga aggcaagact
      961 gatttgctgc ctggattcat ctcgcaacgt acaaaacggc cgttcaaggc tttcctggtg
     1021 cgcggcaagg atggcaaaaa cagcttcgag ttcgaggaac gcaaggccaa gccgcccgcc
     1081 aggggtgaag cgacggtcgg cgcaggcgat gatgccgaag ccagcgccag accggcagtg
     1141 acagccaaaa aacctgccgc ccggaaaccg gcggtaaaga agacggcatc cagaaaagcg
     1201 gcggcagcct gatatgctgt tcgccgacgc ggtataatag cgcgcagttg ggtggtgcgc
     1261 ctttttccgg actgactggc ggcattgcct gtctccgcgc ttttttggtc gtgctcctgt
     1321 cgttcctgag aagtcggccc atcccgtcct tttttgcgtt ccctggcgga cattgttgca
     1381 gcttgctgac atctttttcg cttgccgggc tttacctgga aaccatcatg gctaaagaag
     1441 aattacttga aatgaacggc ctcgtcgaag aagttttgcc tgactcgcgc tttcgtgtcc
     1501 atctggaaaa cggtcatcag ctgattgcct atacttccgg caagatgcgt caaaatcata
     1561 tccgcatcct ggccggtgac aaagtcacgc tggagctttc gccttacgac ctgagcaaag
     1621 gccgcatcat ttttcgccat ctggaaaaac gcgggccagt tggcggcaac cagcagcgcc
     1681 gccgttacta ggcagggtat cagtcgaaaa taccggtaaa atgcgtctat cgctgcaaaa
     1741 ttatttgaaa cttggaagcg gctttgaaag tatcgcttcg ggagtacatt caagaaccgg
     1801 ttgcgtcata tctgttttac cctgatcatg tttactccac gcactgcgga agtcgtccgc
     1861 aataccaacg agacgcaaat tcgcgtcgcg ctcaacctgg atggcgtcgg ccggcaaaag
     1921 ctggataccg gtgtgccttt tcttgatcat atgctggacc aggtcgcgcg ccatggtttg
     1981 atcgacctcg acatccacgc cagaggtgac ttgcacatcg atgcgcatca cacagtggaa
     2041 gacgtcggca ttacgctcgg tcaggctttc gccaaggcca tcggtgacaa gaagggtatc
     2101 tgccgctatg gtcatgctta tgtgccgctc gatgaagcgt tgtcgcgcgt ggtgatcgat
     2161 ttctctggcc gtcccggtct ggaatatcac attccgtttg cccgtgcgat gatcggcagt
     2221 ttcgacgttg atctcaccag cgaattcttc catggctttg tcaatcatgc gcaagtcacg
     2281 ctgcatatcg acaatctgcg cggcgccaat gcgcaccatc aatgcgaaac cgtattcaag
     2341 gcttttggcc gcgccctgcg catggccatc gagctcgacc cgcgtgcggc gggaagcatt
     2401 ccttccacca aaggcagcct gtagacgaaa catcctgttt tgctgctcgc actttctgtg
     2461 cttttgcatg cttcgatatg aataaaatcg ttgtagtgga ttacgggatg ggcaatctgc
     2521 gctcggtcgc gcaggctctg tgccatgtcg cccccgaagc cgacgtgtgt atttctggcg
     2581 tagtcgccga tatccggtcg gcggatcgta tcgtgctgcc ggggcagggc gccatgccgg
     2641 actgcatgcg ctgtttgcgc gaatccggct tgcaggaggc ggtcgttgcc gcgacgcaca
     2701 gcaagccttt gctgggcgtg tgcgttggcg agcagatgct gttcgactgg agtgaagaag
     2761 gcgatacgcc tggtctcggc ctgttgccgg gcaaggtagt gcgctttcgg ttcgatggca
     2821 tggtgcagga agatggttcg cgcttcaagg tgccgcaaac gggctggaac cgtgtgcatc
     2881 agcgagccgc gcatccgctg tgggacggca tcggcgagca ggcatatttc tatttcgtgc
     2941 atagttacta tgcgcagccg ggcgagtcgg cgcacattgt cggcgaaacc gtctatggtc
     3001 aagcttttgc ctgcgctgtg gccagtggca atatttttgc aacacaattc catccggaga
     3061 aaagtgctgc cgcaggtttg caactgtatc ggaatttcgt acactggaaa ccttgattct
     3121 tcccccgcgc tatttttcgc tcaaccaagt aagtcaatgt ctacttacga tttttaagtt
     3181 tacgtagcca tgctgctgat acctgccatc gacctgaaag acggtcattg cgtgcgcctg
     3241 aagcaaggcg atatggacca agccaccgta ttctctgaaa accctgccga catggcgcag
     3301 cattggctgc tgcagggcgc gcgtcgcctg catctggttg acctgaacgg cgcctttgcc
     3361 ggcaagccga agaatgaacc ggccgtcaag gccattctca gggcggtacg cgacttcgct
     3421 gccgacaacc atggcgagga aatccccgtg cagctgggcg gcggtattcg cgatctcgac
     3481 accatcgagc gttatctcga tgatggcttg tcctatatca tcatcggcac cgctgcggtc
     3541 aaaaaccctg g
//
LOCUS       DASRDH010000126         5638 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_591126,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000126 DASRDH010000000
VERSION     DASRDH010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 5638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5638
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_591126"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="VFA14_02900"
     CDS             <1..336
                     /locus_tag="VFA14_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007881779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HZS80362.1"
                     /translation="HFRPEFINRVDEIVVFHALDAKNIGAIAKIQLHILETRLAKMDI
                     GLVVSDVGLQKIAEAGFDPVYGARPLKRAIQQEIENPLSKLILQGKFGPRDTVHVAVE
                     NGKFSFKAH"
     gene            complement(395..1318)
                     /locus_tag="VFA14_02905"
     CDS             complement(395..1318)
                     /locus_tag="VFA14_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004189234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZS80363.1"
                     /translation="MVYVMLELRHLKTLQALKEAGNLLRAATLLNVTQSALSHQIKQL
                     EDHHGTPLFERKSAPVRFTPAGCRLLKLADEVLPRIAEAERDLARLQQGEAGQLRIAV
                     ECHTCFDWLMPSMDVFRKRWPEVDMDIVSGFQADPIGLLHAYRADIAIVADIDADEQV
                     DYHALFRFEIVALLAHDHPLAHKDHLTAEDFASDTLITYPVPDDMLDIVRQVLKPAGI
                     TTQRRTTELTVAMLQLVASRRGIATLPLWAAQNYLNRNYVLAKRITPDGLTGRLYAAC
                     LPELSGRSYLADFIAATRETSYLNLPNVALL"
     gene            1418..3715
                     /gene="metE"
                     /locus_tag="VFA14_02910"
     CDS             1418..3715
                     /gene="metE"
                     /locus_tag="VFA14_02910"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007881782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HZS80364.1"
                     /translation="MALLPAAHVAHVLGFPRIGAHRELKFAMESFWKGESDEEALRAT
                     GKALRARHWQAQAAAGMDYITVGDFAWYDHVLGMLALLGALPARFRLDAGKLDLAAYY
                     TLARGDKDHAAMEMTKWFDTNYHYLVPEWTPELCFDGGVDWLFDEIAEAQSQAHAVPV
                     KVALLGPLSLLYLGKVKSGLSHKLDLLPKVIAGYEKLLARLRGVGVALVQIDEPVLAL
                     EPERQWLDAFLPVYRALSKTASALLLTTYFDTVSEHAALLRSLPVAGVHLDGVRGVTQ
                     LQEFADEWPQDKVLSVGIVDGRNIWRCDLDAALALLKPLHARLGERLWVASSCSLLHV
                     PVDLAHELKLDEELKSWLAFATQKLDEIFALRQALNGNAGTVAGQFVAARAALATRRA
                     SPRTRNALVQKRLQAVIDADAERSSAFPVRLTAQQQRWNLPAFPTTTIGSFPQTLQIR
                     QARAAYRRGEISHLDYLGKMRDEIRLVVEKQEALGLDVLVHGEAERNDMVEYFGEQLW
                     GYAFTANGWVQSYGSRCVKPPLIYGDVYRPEAMTVGWSQFAQSLTQKPVKGMLTGPVT
                     MLQWSFVRDDQPRATTALQIALALRDEVLDLEKAGIGMIQIDEPAFREGLPLKARDWE
                     AYLDWAVRAFRISAAGVKDDTQIHTHMCYAEFNDILPWIAAMDADVITIETSRSDMEL
                     LDGFGRFKYPNDIGPGVYDIHSPRIPDIDDMARLLEKARSVIPDQRLWVNPDCGLKTR
                     NWPEVDEALARMVQAAHKLRETLDA"
     gene            complement(3757..>5638)
                     /locus_tag="VFA14_02915"
     CDS             complement(3757..>5638)
                     /locus_tag="VFA14_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013234095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FUSC family membrane protein"
                     /protein_id="HZS80365.1"
                     /translation="RTLPLQFAALMVMTLSMENTLPFKAAILNTGLFFTGGIGYLTYA
                     MIVAFFLRRRAKQQVLAEALFELACYLRIKAGFYDARSDTQAQFSLLARQQIVLTDKL
                     QTSRDLVLRDLHTKQDGLLVQVHLSILDLYEQVLSTHADHAMLHKYFDDSDVMIFLRD
                     LADKTAKDIESIAYAVTRKRASTPSVNYKAELRAIQYELQQAQQGEIPDDAITVLRVA
                     YSQIQGIIELISQLHLATQTPVDPIRVLPGSDMTPFLTQQKYKASLLRTHLRWQSPIF
                     RFSIRVALAVSAGLWVASYLPYMAHGYWIVLTIVIILKPNFSMTRQRMSDRLSGTIIG
                     CLLTTLILHFIYEPAGLLAVLFIASIAAPAFIYVKYRYTAIAATVQILMLLNLLLPAG
                     HQGVVSERLIDTIIGVAIATLFSYVLPSWEYRDLPLLLKNVLHTSHRYIAASCDIMAG
                     KVGDDFFYRVCRKSFMDSLSTLISAQVRMMDEPISKQLALPEINRFVVQNYLVAAHIA
                     ALRMLLRSHTEGLPRESVNQLITQIYQNATMYLQQAAGIWPPPGNISAAGPDEEGIPP
                     ASLTEAATADGSIPTITPEVTTWPSWYTFQHRSMLLNDDLREIATQSGAIANILHRTE
                     HD"
BASE COUNT         1111 a         1489 c         1832 g         1206 t
ORIGIN      
        1 catttccgtc cggagttcat caaccgggtc gatgagatcg tggtgttcca tgcgctcgat
       61 gcaaaaaaca tcggcgccat tgccaagata cagctgcata ttctggaaac gcgcctagcc
      121 aagatggata tcggtctggt ggtatcggat gttggcttgc aaaagattgc tgaggccggg
      181 ttcgatcctg tttatggtgc gcgcccgctc aagcgggcca tccagcagga aatcgagaac
      241 ccgttgtcga agctgattct gcaaggaaaa ttcggtccca gggataccgt gcatgtggcg
      301 gtcgaaaatg gtaagttttc gttcaaggcg cattgaaatg acttgatgat gccctgagcc
      361 agatgtggca ggcgctcggt cagaatcagc ggctctacag caaagccaca ttgggtaagt
      421 tcagatagct ggtctcgcgt gtggcggcaa tgaaatctgc cagatatgac ctgccggaca
      481 actccggcag gcacgccgca tacaggcgcc ccgtcaggcc gtccggcgtg atgcgtttgg
      541 ccagtacata attccggttc agataatttt gcgcagccca taaaggcagc gtggcgatgc
      601 cgcgccggct ggcgaccagt tgcagcatgg cgacggtgag ttcagtggtg cgccgctgcg
      661 tggtaatgcc ggccggcttg agtacctggc gcacgatatc gagcatgtcg tcggggactg
      721 gataggtaat gagcgtgtcg ctggcgaagt cttccgccgt cagatgatcc ttgtgcgcca
      781 gcggatggtc gtgcgccagc agggcgacga tttcaaaacg gaacaaggca tggtaatcga
      841 cctgttcgtc agcatcgatg tcggccacga tggcgatgtc ggcgcggtac gcgtggagca
      901 ggccgatcgg gtcggcctgg aaaccggaaa cgatgtccat atcgacttcc ggccagcgct
      961 tgcggaatac atccatggac ggcatcagcc agtcgaagca ggtatggcat tccaccgcaa
     1021 tgcgtaactg gccggcttcg ccttgctgca ggcgggccag gtcgcgttct gcctcggcaa
     1081 tgcgcggcag cacctcatcg gccagtttga gcaggcggca gccagcgggg gtgaaacgta
     1141 ccggcgccga tttgcgttcg aacaatggcg tgccatgatg atcttcgagc tgtttgatct
     1201 gatgcgatag tgccgattgc gtgacattga gcagggtggc ggcgcgcagc aggttgccag
     1261 cttccttgag cgcttgcagg gttttgagat gacgaagttc cagcatcaca tacactatga
     1321 ataaaactca agttgattct taaaatatat cgtttgaatc atagttgatg ttcacgcaac
     1381 ataccagttc ccgggttcat tatcaggaga gttggtcatg gctttattac cggcagcaca
     1441 cgtcgcccat gtattgggtt ttccgcgcat cggcgcgcac cgtgaattga aattcgcgat
     1501 ggaatcgttc tggaagggcg agtccgatga ggaggcattg cgcgccaccg gcaaggcgct
     1561 gcgcgcgcgt cactggcagg cgcaggcggc agccgggatg gattatatca cggtcggcga
     1621 ttttgcctgg tacgaccatg tgctgggcat gctggcattg ctgggcgctt tgcccgcgcg
     1681 tttcaggctg gatgccggta agcttgatct ggctgcctat tacacgctgg cgcgcggcga
     1741 caaggaccac gccgcgatgg aaatgacgaa gtggttcgat accaactatc actatctggt
     1801 gccggaatgg acgccggaac tgtgtttcga cggcggcgtc gattggctgt tcgacgagat
     1861 cgccgaggcg cagtcacagg cgcatgccgt tcccgtcaaa gtcgctctgc tcgggccgct
     1921 gagcttgctg tatctcggca aggtcaagtc gggcctgtcg cacaagctgg atttgttgcc
     1981 caaggtgata gccggttatg aaaagctgct ggcgcgtctg cgcggcgtgg gagtagcgct
     2041 ggtgcagatc gacgagccgg tgctggcgct ggagcctgag cggcaatggc tggatgcctt
     2101 tttgccggtt taccgcgcat tgagcaagac ggcatctgcg ttgttgctga cgacttattt
     2161 cgatacggtc agcgaacacg ccgccctgct gcgcagcctg ccggttgccg gcgtgcatct
     2221 ggacggcgtg cgcggcgtca cgcaattaca ggagtttgct gacgaatggc cacaggacaa
     2281 ggtactgtcg gtgggtatcg tcgatggccg taatatctgg cgttgcgacc tcgatgccgc
     2341 gctggcgcta ttgaagccgc tgcatgccag gctgggcgag cgtttgtggg tggcatcgag
     2401 ctgttctctg ttgcatgtgc cggtcgatct ggcccatgag ctcaaactgg atgaggaact
     2461 caagagttgg ctggcgttcg cgacacagaa actggatgag atcttcgcgc tcaggcaggc
     2521 gttgaacggc aacgccggta ccgtcgctgg ccagtttgtg gcagcgcgcg cggcactggc
     2581 gacgcggcgc gcttcgccgc gcacacgcaa tgcgctggta cagaagcgtc tgcaagccgt
     2641 gattgacgcc gatgctgagc gcagctccgc ttttccggtg cgcctcacgg cgcagcaaca
     2701 gcgctggaat ctgccggcct ttccgaccac taccatcggc tctttcccgc aaaccttgca
     2761 gattcgccag gcgcgcgcag cctacaggcg tggcgaaatc agccatttgg attatctggg
     2821 caagatgcgc gatgaaatcc gcctggtggt cgagaagcag gaagcgctcg gcttggatgt
     2881 gctggtgcat ggcgaagcgg aacgcaatga tatggtcgaa tatttcggcg aacagttgtg
     2941 gggctacgct ttcactgcca acggttgggt gcagagttac ggatcgcgtt gtgtcaagcc
     3001 gccgctcatc tacggcgatg tgtatcgtcc ggaagccatg acggtaggct ggagtcagtt
     3061 cgcgcagagc ctgacgcaaa aaccggtgaa gggcatgctg accggcccgg tgacgatgct
     3121 gcaatggtcg ttcgtgcgcg atgatcagcc gcgtgcgacg actgcgctgc aaatcgcgct
     3181 ggcattacgc gatgaagtcc tcgacctgga aaaggccggc atcggcatga tacagatcga
     3241 cgaaccggca ttccgtgaag gcctgccgct caaggcgcgc gattgggagg cgtatctcga
     3301 ctgggcggta cgcgcattca ggatcagcgc cgccggcgtc aaggacgaca cccagatcca
     3361 tacgcacatg tgctatgcgg aattcaatga catcctgccg tggatcgccg ctatggacgc
     3421 tgatgtgatc acgatcgaga cttcgcgctc ggatatggag ctgctggacg gcttcggccg
     3481 gttcaagtat cccaacgaca tcggtcccgg cgtctacgac atccactcgc cgcggattcc
     3541 ggacattgat gacatggcgc gcctgttgga gaaggcgcgc agcgtgattc ccgatcaacg
     3601 tttgtgggtc aatccggatt gcggtctgaa gacacgcaac tggcctgaag tcgatgaagc
     3661 gctggcgcgc atggtgcagg cggcgcacaa gctgcgcgaa acgctcgatg cctagagtag
     3721 tgcgctcctg atttctgcga ggtcgtcaac acccttctaa tcgtgctcgg tccggtgcag
     3781 gatatttgct atggcgccgc tttgcgtcgc gatttcccgc agatcgtcgt tgagcagcat
     3841 cgaacggtgc tggaaggtat accagctcgg ccatgtggtg acttctggcg ttatcgtggg
     3901 gatgctgcca tcggctgtcg cggcttccgt caacgaagcg ggcgggatac cttcctcatc
     3961 cggcccggcg gcagagatgt tgccgggtgg gggccagatg cctgctgctt gttgcagata
     4021 catggtggcg ttctggtaga tctgcgtgat cagctggttg accgactcac gcggcaagcc
     4081 ttcagtatgg ctgcgcaata gcatgcgtag tgctgcgatg tgggccgcga ccagatagtt
     4141 ttgcaccacg aagcgattga tttcgggtaa ggcgagctgc ttactgatcg gttcatccat
     4201 catacggacc tgggccgaaa tcagcgtcga gagactgtcc atgaaacttt tgcggcagac
     4261 gcggtaaaag aagtcgtcgc cgactttgcc cgccatgatg tcgcagctgg ctgcaatgta
     4321 gcgatggctg gtgtgcagca cgtttttgag cagcagcggc agatcgcggt attcccagct
     4381 cggcagcaca taactgaaga gtgtcgcgat agccacgccg atgatggtgt cgatcaggcg
     4441 ttcgctgacc acgccctggt ggccggccgg cagcagcaga ttgagcagca tcaggatttg
     4501 taccgtggca gcaattgcgg tatagcggta tttgacgtaa atgaaggctg gcgcggcgat
     4561 gctggcgatg aacagaactg ccagcaggcc ggccggctcg tatatgaagt gcagaatcag
     4621 ggtcgtgagc aggcagccga tgatggtgcc ggacagacgg tcgctcatac gctgtctggt
     4681 catgctgaag ttgggtttga ggatgatgac gatggtcaac acgatccagt aaccgtgcgc
     4741 catatatggc aggtaactgg ccacccacag gccggccgac accgccagcg ccacgcggat
     4801 ggagaaacgg aagatcggcg actgccagcg caggtgggtg cgcagcaggc tggccttgta
     4861 tttttgctgg gtcaggaacg gtgtcatgtc ggatcctggc aggacgcgga tcggatcgac
     4921 cggcgtttgc gtggccaggt gtaactggga gatcagttcg atgatgccct ggatctggct
     4981 gtacgcgacg cgcagcacgg taatggcatc gtctggaatc tcgccttgtt gagcctgctg
     5041 caattcgtac tgtatggcac gcagctctgc cttgtagttg acgctcggcg tcgaggcgcg
     5101 tttgcgagtg acggcgtagg caatggattc gatgtctttg gctgtcttgt cggccagatc
     5161 gcgtaggaag atcatcacat cgctgtcatc gaagtacttg tgcagcatgg cgtggtcggc
     5221 atgggtggag agaacctgtt cgtaaagatc gagtatgctt agatggacct gcaccagcag
     5281 gccgtcctgc ttggtgtgaa ggtcgcgcag gaccaggtcg cgcgaggttt gcagcttgtc
     5341 agtcagcacg atctgctgac gcgccagcag gctgaattgc gcttgggtat cgctgcgcgc
     5401 gtcgtagaaa ccggccttga tgcgcaggta gcacgctagc tcgaacaagg cttcggccag
     5461 cacttgctgt ttggcgcgcc gtcgcagaaa gaatgccacg atcattgcat acgtcaggta
     5521 gccgatgccg ccggtgaaga ataggccggt attcaggata gctgccttga acggcagggt
     5581 gttttccatc gataaggtca tcaccatcag tgctgcaaat tgcagcggca gcgtcctt
//
LOCUS       DASRDH010000127         2138 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1625994,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000127 DASRDH010000000
VERSION     DASRDH010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1625994"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2085)
                     /locus_tag="VFA14_02920"
     CDS             complement(<1..2085)
                     /locus_tag="VFA14_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS80366.1"
                     /translation="MAFDVNGAKAAGYSDTEIADHLAGTANFDAAGARKAGYSDAELI
                     AHLVPAKASAAPPTSAGAVDEFGRNIGSVKFPTGQTQIGADFAAAGQGAVSGAGEAIA
                     GIGRQLPEAPGARTIQQQIAVMDAIDAKRPVPQDQDVIGYQFFTPEQKAKAREDFTAA
                     GVKADAEAEKREPNALIRAGTAMQEAAPGIFPVDPALEGRGTRVGRMVGGMVPALGAS
                     AVGALGGPVGAVMAGAAAIGAQTYDGTYQEAISKGSTPAEAESAAGKAAMAQMALTSV
                     PISRVLPLIPVPLREGFMKTVANVGANFIQRGGEMSAGNTLASIAQNYIRSQTTDPGA
                     PVFEGMGDTALDSFLAGLVVHGATSGAGAAVRGVRDIATAKDVDGAIAAAGSAVKDEP
                     APGSWGEIFPKGEEIPTITVTPQGASGPLQPAGAMVTPQSAGAAASRQGTPSGAFGRT
                     AREITGQHADMELADLLRTPQKGDARDIIPGARQTKAEIELEPSVSRTSKGYRQEFRE
                     GYNDLEKDNNEIYHKWIDDVSPSGEQVGTMRDVREANWKANEKTAFGPNRNGEPASTA
                     PIVQHIVNVLDDPIEAYNSGLKAAFRPILRELVDADGAPVELGIKRMYGLRQEMRRQT
                     TAMAADPKLAHMRDQMGSLMAVTDAVIADKSPAYQAMLDDYSARSRQIDAATRLQDMA
                     LKVATGPDRMITF"
BASE COUNT          381 a          729 c          593 g          435 t
ORIGIN      
        1 gaaggtgatc attctgtcgg gaccggtagc aaccttcagt gccatatcct gaagtctcgt
       61 ggctgcatcg atctgtctcg acctcgcact gtagtcatcc aacatggctt gataagcagg
      121 ggatttatcg gcgatgaccg catcagtcac agccatcaag ctgcccattt gatcacgcat
      181 atgcgcaagc ttggggtccg ccgccatagc tgtagtttgc cgacgcatct cctgccgaag
      241 accatacatg cgcttgatac caagctctac cggagcgccg tcagcatcaa caagctctcg
      301 cagaattggc cgaaacgcag ctttcaggcc ggaattgtaa gcttcaatag gatcatccaa
      361 tacattcact atgtgctgga caataggcgc ggttgaggca ggctcaccat ttcggttcgg
      421 cccgaaagca gtcttctcat tcgccttcca attcgcctct cgcacgtccc tcatcgttcc
      481 aacttgttcg cctgacggcg agacatcgtc aatccatttg tgataaattt cgttgttgtc
      541 cttctcaaga tcgttgtagc cctcacggaa ctcctgccgg taacccttag aagtgcgcga
      601 caccgaaggt tctaactcga tctctgcctt ggtctgccgc gcacctggga tgatatcgcg
      661 cgcatcgccc ttctgtggtg tccgcaaaag gtcggccagt tccatatcgg cgtgctgacc
      721 tgtgatctcg cgcgcggtgc ggccgaatgc gccactgggc gtcccctgcc gcgacgcggc
      781 ggcgcccgca gattgcgggg ttaccatggc gcctgcgggc tgtagcggcc ctgacgcccc
      841 ctgaggcgtc acggtgatgg ttggaatctc ctcacccttc gggaatatct caccccagct
      901 cccaggcgcc ggctcgtcct tcaccgcgga cccggccgcc gcgatcgcac catcgacatc
      961 cttcgcagtc gcgatgtccc taacaccacg caccgcggca ccagcgccgc tggtcgcacc
     1021 gtggacgacc agcccagcta ggaagctgtc tagcgccgtg tcgcccatgc cctcgaacac
     1081 cggcgcgccg gggtcagtcg tctgtgatct gatgtagttc tgcgcgattg aggcgagcgt
     1141 attgccggcg ctcatctcgc caccgcgctg gatgaaattc gcgccgacat tcgcgacggt
     1201 cttcatgaaa ccttcgcgca gcggcaccgg gatgagcggc agcaccctac tgattgggac
     1261 cgacgtcagt gccatctgag ccatcgctgc cttgccagcc gccgactcgg cctctgctgg
     1321 cgtggatccc ttgctgatcg cctcctgata ggtgccatcg taggtctgcg caccgattgc
     1381 cgcggccccc gccatgacag ctcctacagg gccgcctagc gctccgacag cagacgcccc
     1441 tagtgcaggg accatgccgc cgaccatgcg ccccacgcgc gtcccacggc cctccagggc
     1501 cgggtcgacc gggaaaatcc ctggcgccgc ctcctgcatc gccgtacctg cgcggatcag
     1561 cgcattcggc tcgcgcttct cagcctccgc gtcggctttg acacccgcag cggtgaagtc
     1621 ctcccgcgcc ttcgccttct gttcgggcgt gaagaactga tacccgatga catcctgatc
     1681 ctgcggcact ggacgtttgg cgtcgatcgc atccattacg gcgatctgct gctgaatggt
     1741 ccgcgcccca ggagcttcgg gcaactggcg accgatgccc gcgatggcct cgccagcccc
     1801 gcttaccgcg ccctggcctg cagcagcgaa atcggcgcca atctgggtct gaccagtagg
     1861 gaatttgacc gacccaatgt tacggccaaa ctcatcaact gctcctgcgg acgttggcgg
     1921 ggcggcggac gccttcgcag gaactaaatg cgcaatcaac tcagcatcgg agtatccggc
     1981 cttacgcgcc ccagccgcgt caaaattcgc cgtgcctgcc agatggtcgg caatctcggt
     2041 atccgagtag ccggccgcct tggcgccatt cacatcaaat gccattgtta tctatcgaag
     2101 ctcgacaagg gaggccgtga gctgggcgcc gttccacc
//
LOCUS       DASRDH010000128         5543 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_664774,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000128 DASRDH010000000
VERSION     DASRDH010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 5543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5543
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_664774"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="VFA14_02925"
     CDS             complement(<1..182)
                     /locus_tag="VFA14_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007881447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein"
                     /protein_id="HZS80367.1"
                     /translation="MVAASAASVVFIGLIFGFSLAGELPFMFRLVVATACILAASLAF
                     WLALRNCKAVGLHISGT"
     gene            complement(344..1585)
                     /gene="fabF"
                     /locus_tag="VFA14_02930"
     CDS             complement(344..1585)
                     /gene="fabF"
                     /locus_tag="VFA14_02930"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008327974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HZS80368.1"
                     /translation="MSRSRERRVVITGLGCISPVGNTIADTWGAIREGKSGIATITKF
                     DVTPFATHFAGEVKGFDVEDHIPAKEARHMDTFIHFGMVAGMQAIQDSGLVVTDENAE
                     RIGVIVGSGIGGLPLIEQTKEDLLERGPRRISPFFIPASIINMISGNLSIRYGLKGPN
                     LAIVTACTTGLHCIGSASRMIQYGDADVMIAGGAESSVSPLGIGGFAAARALSTRNDD
                     PATASRPWDKDRDGFVLGEGAGVMVLEEYEHARARGANIYAEILGFGMSSDAYHMTAP
                     DLDGPRRCIVSALRDAGVNPDEVQYLNAHGTSTPLGDKNETDAVKAVFGAHAYRMVVN
                     STKSMTGHLLGGAGGLESVFSVLALHHQISPPTINIFHQDPECDLDYCANQARDMKID
                     IAVKNSFGFGGTNGALVFGKV"
     gene            complement(1616..1855)
                     /gene="acpP"
                     /locus_tag="VFA14_02935"
     CDS             complement(1616..1855)
                     /gene="acpP"
                     /locus_tag="VFA14_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012735022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HZS80369.1"
                     /translation="MSDIEQRVKKIVAEQLGIAEAEIKAESSFVDDLGADSLDTVELV
                     MALEDAFEMEIPDEQAEKITTVQQAIDYAKAHVKA"
     gene            complement(1977..2732)
                     /gene="fabG"
                     /locus_tag="VFA14_02940"
     CDS             complement(1977..2732)
                     /gene="fabG"
                     /locus_tag="VFA14_02940"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HZS80370.1"
                     /translation="MSACNLENQIALITGASRGIGRAIAQELACRGAKVIGTATSEAG
                     VDVIDKYLEQLQPGCGKGIVLDVRDVAACNAVVGAIQKKCGGLSILVNNAGITQDQLA
                     MRMKEEEWDDVIATNLTAVGRLSRAVLRGMMKAKYGRIINITSIIGSAGNPGQMNYAA
                     AKAGVAGMSRALAREVGSRNITVNCIAPGFIDTDMTRELPEEQTAALLQQIPLGRLGA
                     PEDIAAAVAFLASAQAGYITGATLHVNGGMYMS"
     gene            complement(2729..3661)
                     /gene="fabD"
                     /locus_tag="VFA14_02945"
     CDS             complement(2729..3661)
                     /gene="fabD"
                     /locus_tag="VFA14_02945"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008327970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HZS80371.1"
                     /translation="MTRFAFVFPGQGSQAIGMLNGFADNAIVKATVAEASDVLRFDVG
                     ALIAEGPKADLDLTVNTQPVMLTAAVAFYRAWLAAGGQAPALVAGHSLGEYSALVAAG
                     VIAFKDALPLVRFRAQAMQEAVPAGQGGMAAILGLSDDDVRAACAQAAQGEVVEAVNF
                     NAPAQVVIAGHKAAVERACDIARTKGARRALPLPVSAPFHSSLLRPASDRLQTYLANV
                     AFLAPQLPLINNVDVVIVNDPVAIKDALVRQAASAVRWVETIRKMADQGMTHVIECGP
                     GKVLTGLTRRIDDGLIGEVIVDQTSLDQVLELLR"
     gene            complement(3719..4705)
                     /locus_tag="VFA14_02950"
     CDS             complement(3719..4705)
                     /locus_tag="VFA14_02950"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HZS80372.1"
                     /translation="MTLCSRIVGTGSYLPPRRVTNEELARQLAAKGIETSDEWIVSRS
                     GISARYYAEPDVLSSDLAVMAAQRALEMAVLQADDIDLIIVATSTPDHFGGFPSTACV
                     VQRKLGMTNGSAAMDVQAVCSGFVYAMSVADSFIKSGAYKNVLVIGAEVFSRILDFAD
                     RSTCVLFGDGAGAIVMTASDRPGVLASKLHADGSYGHILCVPGNINAGAIAGNAYLHM
                     DGPAVFRLAVSVLDKVAREVLSIAQIDAKQIDWLIPHQANIRIMQGTARKLGLPLEKM
                     VVTVDEHGNTSAASIPLALDRAVRDGRVKPGHHVMMEGVGGGITWGAALVRM"
     gene            complement(4721..>5543)
                     /gene="plsX"
                     /locus_tag="VFA14_02955"
     CDS             complement(4721..>5543)
                     /gene="plsX"
                     /locus_tag="VFA14_02955"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007881441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HZS80373.1"
                     /translation="DSSMRVAVNLLKEGQADAAVSAGNTGALMAVSRYVLKTIPGVDR
                     PAICTLIPNQKDGPIYMLDLGANVDCEPQHLYQFALMGSALVAAMEGKPRPTVGLLNV
                     GEENIKGNEVVKQASWLLHAGHEKGILNFYGNVEGNDIFEGTTDIVVCDGFVGNVTLK
                     ASEGLGRFLKIALATEFKNGFFNRLGALIAYGALKALSRRLDPSRYNGASLLGLRGLV
                     FKSHGGADVYAFEWAIRRAYDAAKYGVLSRITTTMVELIPQVEAPAVGCASSPDE"
BASE COUNT         1336 a         1692 c         1398 g         1117 t
ORIGIN      
        1 gtccctgaaa tatgtaatcc gacggcttta caattgcgca gcgccagcca gaatgctagc
       61 gacgcagcca gaatgcacgc ggtagccaca accaagcgga acatgaaagg caattccccc
      121 gccagggaaa aaccgaaaat gaggccaatg aagacgacgc tggcagcaga tgccgccacc
      181 acggtcaata gcatacggga agattgaata tctgcggaga ctgctatcga catataaaaa
      241 tggaaacgaa gaaaccgtgt ttacgatccc atcggaagtc ttccgggcat cttttcagga
      301 tgggacggcc ccaaaggaga tcgtaaacac cttcccatgt ctttcatacc ttaccgaaca
      361 ccagcgcgcc gttggtacca ccgaatccaa atgaattttt gacggcgata tcaattttca
      421 tatcgcgcgc ctgattggcg caataatcaa gatcgcattc aggatcctga tgaaagatgt
      481 tgatcgtcgg cggagagatt tgatgatgta gcgccaatac ggagaacacc gactccaggc
      541 cgccagcgcc gcccagcagg tgaccggtca tcgacttggt cgaattgaca accatcctgt
      601 aagcatgagc gccaaacaca gccttgacag catccgtttc attcttgtcg cccagcggcg
      661 tcgacgtgcc atgcgcattc aaatactgca cttcatccgg attcacgccg gcatcacgca
      721 gggccgacac tatgcagcgg cgcggaccat ccagatcggg cgcagtcatg tgataagcgt
      781 cactgctcat gccgaaaccg agtatttctg catagatatt ggcgccgcgc gccctggcat
      841 gttcgtactc ttccagcacc atcacaccgg cgccttcgcc gagaacgaaa ccatcacggt
      901 ctttatccca cggacgggaa gcagtcgccg gatcgtcatt acgcgtcgac agggcacgcg
      961 ccgcagcgaa accaccaata cccagcggcg aaacactgga ctcggcgcca ccggcgatca
     1021 tgacatcggc atcaccatat tgaatcatgc ggctggccga gccgatgcaa tgcaagcctg
     1081 tcgtacaggc agtcacgatg gccagattag ggcctttcag accatacctg atcgacagat
     1141 taccggaaat catgttgatg atcgatgccg gaatgaaaaa tgggctgatg cggcgcggac
     1201 cgcgttcgag caaatcttcc ttcgtctgct cgatcagcgg caaaccgccg ataccggaac
     1261 cgacgatgac accgatacgt tcagcattct catcggtgac gaccaggccg ctatcctgta
     1321 tggcctgcat cccggccacc atgccgaaat ggatgaaggt atccatatgg cgcgcttctt
     1381 tggcgggaat atggtcttcg acgtcgaagc ccttcacttc acctgcaaaa tgcgtggcga
     1441 aaggggtgac gtcaaacttc gtgatggtgg caatgcccga tttgccttcc ctgattgccc
     1501 cccatgtgtc ggcaatcgta ttgccaactg gcgaaatgca acccaaaccg gtaatgacta
     1561 cgcgacgttc acgtgagcgg ctcaaacagt tctcctggcg tcgttcaaaa ccggctcaag
     1621 ccttgacatg cgccttggcg taatcgatcg cctgctgcac ggttgtaatt ttttcagcct
     1681 gttcgtcagg aatttccatt tcgaatgcat cttccagcgc cataaccagt tcgacggtat
     1741 cgagcgaatc ggcacccaga tcatcaacga acgacgattc ggccttgatt tccgcttcgg
     1801 cgatgcccag ctgctcagca acgatttttt taacgcgttg ttcgatatcg gacataagtg
     1861 cctccagtga atgggttaca aaagtgcgcg cattttatca ggtttgcgcg ccaaaaattt
     1921 acttttacgt attctcaaaa aatcagcgca ttgccgccaa ctgcatgcaa aggcatttaa
     1981 ctcatataca tgccgccatt gacatgcaag gtcgcaccag tgatataacc ggcctgcgct
     2041 gacgccagaa aagcgacggc agcggcaata tcctcaggcg cgccaaggcg acccagtggg
     2101 atctgctgca acaaagcggc agtctgctcc tcaggcaact ccctagtcat gtcagtatcg
     2161 atgaaacctg gagcgataca attgacagta atattgcggc tgccgacttc acgcgccaag
     2221 gcgcggctca taccggcaac gccggctttg gcggcagcat aattcatctg tcccggattg
     2281 cctgccgagc cgatgatcga ggtgatgttg atgatgcgtc catacttggc cttcatcata
     2341 ccgcgcaata cggcgcgcga cagacgcccc accgccgtca gattcgtggc gatgacatca
     2401 tcccactcct cttccttcat gcgcatggcc agttgatcct gtgtgatacc agcgttattg
     2461 accagaatgg ataaaccgcc gcatttcttc tggatcgcac ccactacggc attgcaggcg
     2521 gcaacgtccc tcacgtcgag aacgatgccc ttgccacaac ctggctgcaa ttgttccaga
     2581 tatttgtcaa tgacgtcgac gccagcctca gaagtcgccg tgccaatcac tttggcgcca
     2641 cgacacgcca gttcctgcgc gatggcgcgg ccgataccac gggaagcgcc ggtgatcagc
     2701 gcgatctgat tttccaagtt gcaagcgctc atcgcaacaa ctccaatacc tggtctaaag
     2761 aagtctgatc gacgatgact tcgccgatca gaccatcatc gatgcgtctg gtcaaaccgg
     2821 tcagcacttt accaggaccg cattcgatca catgggtcat gccttggtcc gccattttgc
     2881 gtatcgtttc gacccaacgc accgcactcg cggcctgacg caccaacgca tccttgatcg
     2941 caacaggatc attgacgata acgacatcga cattgttgat gagcggcagc tgcggcgcca
     3001 agaaagcgac attggcgaga taggtctgca aacgatccga tgcaggcctg agcaaggacg
     3061 aatggaaggg agcggacacc ggcagcggca atgcgcgtct ggcgccctta gtcctggcaa
     3121 tgtcgcaggc gcgctcgacc gcggccttgt ggccggcaat cacgacctga gccggtgcgt
     3181 tgaaattgac ggcctccacc acctcgcctt gcgcagcttg cgcacatgca gcgcgcacat
     3241 catcatccga cagacccaga atcgccgcca taccgccctg tccagcagga acggcttcct
     3301 gcatcgcctg tgcgcgaaaa cgcaccagcg gcaacgcatc cttgaaggca atcacgccgg
     3361 cagccaccag cgcagaatac tcgccgaggc tatgaccggc aaccagcgcc ggcgcctgac
     3421 caccagccgc cagccaggca cgataaaacg caaccgccgc cgtcagcatg acaggttggg
     3481 tattaacggt cagatcgagg tccgctttcg gaccttcggc aatcaacgcg ccgacgtcga
     3541 aacgcaagac atcggaagcc tccgcgaccg tcgccttgac aatcgcgtta tcggcaaaac
     3601 cattcagcat gccaatggct tgcgaacctt gaccgggaaa aacaaaagca aatcttgtca
     3661 tgctctattt tgtcattggc aataaaattc gcagcttgaa atccgtttag aacagattct
     3721 acattctgac cagcgccgca ccccaggtga taccgccgcc aacgccctcc atcatgacat
     3781 gatggcccgg cttgacgcga ccgtcacgca ccgcccgatc caatgccaac ggaatcgatg
     3841 cggctgaagt attaccatgt tcatcgacgg tcaccaccat tttttccaat ggcaggccca
     3901 gcttcctggc ggtaccctgc atgatacgga tattggcctg atgcggaatc agccagtcga
     3961 tttgcttcgc atcgatctgg gcgatactca acacctcacg cgccactttg tccagaacgg
     4021 aaacggccag cctgaagacc gccggtccat ccatgtgcag ataggcattt ccagcaatgg
     4081 cgccggcgtt gatatttccc ggcacacaca ggatgtggcc atagctgccg tcggcatgca
     4141 gtttcgaggc gagcacaccc ggcctatccg atgccgtcat gacgatggcg ccggcgccgt
     4201 caccgaacag cacgcaggtg ctgcggtctg caaaatccag aattcgcgaa aacacttccg
     4261 caccaatcac cagtacgttc ttgtacgcgc cggatttgat gaagctatcg gctaccgaca
     4321 tggcgtagac aaaaccgctg caaaccgcct gtacatccat cgcggcgcta ccgttggtca
     4381 tacccagctt gcgctgcacg acacaggcag tactcggaaa tccgccgaaa tgatccggcg
     4441 tcgatgtcgc cacgatgatc agatcgatat cgtcagcctg caacactgcc atttccagcg
     4501 cgcgctgcgc cgccatcacc gcgaggtcgc tcgacaagac atccggttcc gcatagtagc
     4561 gcgcggaaat accactgcgc gagacgatcc attcatcgga agtctcgatg ccctttgccg
     4621 ccagctgccg cgccagttct tcgttggtca cgcgcctggg cggcagataa ctgccagtgc
     4681 cgacaattct gctgcacaga gtcataccgt tttattccat tcactcatcc ggggaggaag
     4741 cgcaaccaac ggcaggcgcc tccacttgcg ggatcaattc caccatcgtc gttgtgatgc
     4801 gtgacaacac accatatttc gccgcatcgt aagcacgacg aatggcccat tcaaatgcat
     4861 acacatcggc gccaccatgg ctcttgaata ccaggccgcg caagcccagc aaactggcgc
     4921 cattgtagcg ggatggatcg agccggcggg acagggcctt gagagcgccg taggcaatca
     4981 aagcgcccag tctgttaaaa aaaccatttt tgaattccgt ggcaagagca atcttgagga
     5041 aacgccccag tccttccgat gccttcaagg tcacattgcc gacgaagccg tcacacacga
     5101 caatgtccgt ggtgccctcg aagatatcat tgccttcgac attgccataa aaattcagga
     5161 tgcccttctc atgacctgca tgcaataacc aggaagcctg cttgacaact tcattaccct
     5221 tgatgttttc ttcgccgaca ttgagcagac cgaccgttgg tctgggcttg ccttccatgg
     5281 cagcaacaag ggccgaaccc atcaatgcga actgatacag atgctgcggc tcacaatcta
     5341 cattcgcgcc cagatcaagc atatagatcg gaccatcttt ttgattggga atgagagtac
     5401 agatcgccgg ccgatcgaca cccggaatgg tcttcaggac gtagcgggac accgccatca
     5461 atgcaccggt attaccggcg gaaacggctg cgtcagcctg accttccttg agcagattca
     5521 ctgcgacgcg catcgacgaa tct
//
LOCUS       DASRDH010000129         4231 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_931046,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000129 DASRDH010000000
VERSION     DASRDH010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 4231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4231
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_931046"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="VFA14_02960"
     CDS             complement(<1..285)
                     /locus_tag="VFA14_02960"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141072.1"
                     /note="catalyzes the formation of 2-C-methyl-D-erythritol
                     4-phosphate from 1-deoxy-D-xylulose-5-phosphate; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HZS80374.1"
                     /translation="MQAITVLGSTGSIGVSTLDVVGRHPDRYCVYALTAHTRVDALAQ
                     QCARFQPRLAVVGTAQAAQQLQQKLRALQLRTEVLYGSQSLCDVAGAAECH"
     gene            complement(559..1374)
                     /locus_tag="VFA14_02965"
     CDS             complement(559..1374)
                     /locus_tag="VFA14_02965"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HZS80375.1"
                     /translation="MLKTRVITALVLLAVLLPLFFLSDFIAFAVVALAFFAAGAWEGL
                     RLFQLSRPQLWAGVWTAVYAAIVFVSDLPGECGLLGLCVALWAFGFVPALKLGLPPPG
                     TSGDRLLSVAYMLALMGCFIAIVDLFRHAPLYLLSVMAIVWVADIGAYFVGKRCGRRR
                     LAPSISPGKSWEGAVGGWLAVLAIGAATVICLGGDTFSGRLYERYGWPGFVMVMTLLV
                     IAAVVGDLFESQLKRRAGIKDSSSLLPGHGGVLDRIDALIPVLPLAALIDLRL"
     gene            complement(1374..2132)
                     /gene="uppS"
                     /locus_tag="VFA14_02970"
     CDS             complement(1374..2132)
                     /gene="uppS"
                     /locus_tag="VFA14_02970"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013234161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HZS80376.1"
                     /translation="MTYRSSTQVVPQVSAVPRHVAIIMDGNGRWATRRFLPRMTGHVK
                     GVEAVRTVVKACVQRGIDYLTLFAFSSENWRRPAEEVSLLMRLFMTALEREVSKMHAN
                     GICLRVVGDLSRFDDRLQDMVARAEAQTAGNTRLTLTICANYGGRWDIMQAIGRMLEE
                     QSGASGFSEEQLSRHLAMAYAPEPDLFIRTGGEERISNFLLWQLAYSELYFTETYWPD
                     FDETELDKAIDSYRQRERRFGRTSAQVVEQKKAS"
     gene            complement(2173..2733)
                     /gene="frr"
                     /locus_tag="VFA14_02975"
     CDS             complement(2173..2733)
                     /gene="frr"
                     /locus_tag="VFA14_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HZS80377.1"
                     /translation="MSIADVKKNTEQRMGRSIETLRASLAKVRTGRAHTGILDHVQVD
                     YYGSLTQLSQIASLTLLDARTIGVQPYEKKMLAAVEKAIREADLGLNPATQGELIRVP
                     TPPLTEERRKEMVKLVKSEAEDTRVSIRNIRRDANEGLKKLLKDKDCSEDDERRGQDD
                     IQKLTDRFVAEVDKLVTEKEKEVLTV"
     gene            complement(2800..3516)
                     /gene="pyrH"
                     /locus_tag="VFA14_02980"
     CDS             complement(2800..3516)
                     /gene="pyrH"
                     /locus_tag="VFA14_02980"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008117651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HZS80378.1"
                     /translation="MTTPAYKRVLLKLSGEALMGDDAYGINRATIERMVADVAEVSRL
                     GVELAIVIGGGNIFRGVAPGAQGMDRATADYMGMLATVMNSLALADAMRQTGLTARVM
                     SAIGIEQIVEPYVRPKALQYLEEGKIVVFAAGTGNPFFTTDTAAALRGSEIGAEIVLK
                     ATKVDGVYTSDPRKDPAATRYSTISFDEAIAKHLQVMDATAFALCRDQKLPVKVFSIV
                     KPGALRRVVLSEDEGTLVHV"
     gene            complement(3704..>4231)
                     /gene="tsf"
                     /locus_tag="VFA14_02985"
     CDS             complement(3704..>4231)
                     /gene="tsf"
                     /locus_tag="VFA14_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006462541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HZS80379.1"
                     /translation="TVEEKRAELVGRIGENMSIRRFVRFETAGRLVSYLHGTRIGVVV
                     DFDGADEQVGRDVAMHIAAMKPVALSSDLVPAALIEKERSVASLKAAESGKPDDIVAR
                     MVDGAVRKYLKEVSLFDQPFVKNDKQTVEQMLKAANTVLKGFTLFVVGEGIEKKQDDF
                     AAEVAAQVAAARQAQ"
BASE COUNT          927 a         1368 c         1110 g          826 t
ORIGIN      
        1 atgacattcc gcagcaccgg ccacgtcgca caaggactga ctgccgtaca gcacttcggt
       61 gcgcaattgc agcgcccgca gcttttgctg caactgctgc gcggcctgcg ccgtaccaac
      121 cacagcgaga cggggctgga aacgcgcaca ttgttgcgcc agcgcgtcga cgcgtgtatg
      181 cgccgtcagc gcatatacac agtaacgatc gggatgccgc ccgaccacgt ccagcgtcga
      241 aacgccgatc gaaccagtcg agccaagtac ggtaattgcc tgcatgaaat tcctagagcg
      301 tgttaggcac tatttcatag atcccgttcg ctcgagaagg gatgcagcgc aaggtacggc
      361 gcgcggcaca gggcaacacc ctgtgccgcg cgccgcaacg ccagcgccgt tctcgaccga
      421 accctccagg cagccccggg aacgagccgc ctgccgtgtt gcgactcctt accgtagcac
      481 cgctacgccg cgtcgtcgcg ccttgcattc agcccgttcc cggggctgca tgacctatga
      541 aatagtgcct aacacgctct acagccgaag atcgatcaac gccgccagcg gtaaaaccgg
      601 aatcagcgca tcgatcctgt ccagtacgcc gccatgaccc ggcagcaaac tgctgctgtc
      661 cttgatgccg gcgcggcgct tgagttgcga ttcgaacaga tcaccgacca cagcagcgat
      721 aaccagcagc gtcatgacca tgacgaaacc tggccagcca tagcgctcat aaaggcgtcc
      781 tgaaaaagta tcaccaccga gacagattac cgtcgccgcc ccgatcgcca gcactgcaag
      841 ccagccaccg accgcgccct cccaggactt gccaggggaa atcgacggcg ccagcctgcg
      901 ccgtccgcat cgcttgccga caaaataagc accgatatca gccacccaaa cgatcgccat
      961 caccgatagc aggtaaaggg gcgcatgtcg aaacaggtca acgatcgcga tgaaacaccc
     1021 cattagcgcc agcatatagg cgacgctcaa caggcgatcg cccgacgttc ccggcggcgg
     1081 caaaccgagt ttcaaggcag gcacgaagcc aaacgcccac aacgccacgc acaagcccaa
     1141 caggccgcac tcaccgggca ggtcgctgac aaaaacgatc gcggcgtaaa cggctgtcca
     1201 gacgcctgcc cacaactgcg gccgtgagag ctggaacaga cgcagaccct cccaggcgcc
     1261 agccgcaaaa aatgccagcg ctacgacggc aaatgcaatg aagtcactca ggaaaaagag
     1321 cggcaacagg accgccagaa gaaccaaggc cgtgatgaca cgcgttttca gcatcaggaa
     1381 gccttcttct gctcgaccac ctgcgcactg gtacgaccga agcgccgttc gcgctgacga
     1441 taggaatcga tcgccttgtc cagttcagtt tcatcgaaat ctggccaata ggtctcagta
     1501 aaatacagct cgctataggc gagttgccac agcaaaaaat tggaaatgcg ctcttcgcca
     1561 ccggtacgga tgaacaaatc cggctcaggc gcataggcca tcgccagatg gcgactcaac
     1621 tgttcttcgg aaaaaccgct cgcgccagac tgctcctcga gcatcctgcc gatcgcctgc
     1681 atgatgtccc agcggccgcc atagttggcg caaatggtga gcgtcagacg cgtattgccg
     1741 gcggtctgcg cttcggccct ggccaccatg tcctgcaatc tatcatcgaa acggctgaga
     1801 tcgcccacca cacgcaagca aatgccgttc gcatgcatct tgctgacttc acgctccagc
     1861 gccgtcatga acaggcgcat cagtaaagat acttcttcgg caggccggcg ccagttttcg
     1921 gaactgaagg caaacagcgt gagatagtcg ataccacgct gcacgcatgc cttgacgacc
     1981 gtgcgaacgg cttcgacgcc cttgacatgc ccggtcatgc gcggcagaaa acgtctggtc
     2041 gcccaacgtc cgttgccatc catgatgatg gcgacatgcc gcggcactgc tgaaacctgc
     2101 ggcacgacct gggtcgagct tctataagtc atgcaatgtg aaaatccaga tgagcggcca
     2161 atgccgatag ccctacaccg tcaacacttc tttttctttt tccgtcacca gtttgtcgac
     2221 ttccgcgaca aacctgtcgg tcagcttctg gatatcgtcc tggccgcgac gctcgtcatc
     2281 ttccgagcaa tccttgtctt tgagcagctt tttcaagcct tcgttggcat cgcgccggat
     2341 gttacggatc gagaccctgg tgtcttcagc ttcgctcttg accagcttga ccatctcctt
     2401 gcggcgctct tcggtcaacg gcggcgtcgg cacacggatc aactcgccct gtgttgccgg
     2461 attgaggccc agatcggctt cacgaatcgc tttttcgacc gctgccagca ttttcttttc
     2521 gtaaggctgc acgccgatgg tgcgcgcatc gagtaaagtc aaactggcga tctgcgacaa
     2581 ttgcgtcagc gaaccgtaat aatccacctg cacatgatcc aggataccgg tatgcgcgcg
     2641 gccggtgcgg actttagcca agctggcgcg caacgtctcg atcgacctgc ccattctttg
     2701 ctctgtattc ttcttgacgt cagcgatgct catgctgctc tcctatcatc taaaaccact
     2761 tttagaacgt gtttacgaca tgagtacgtg aacacgctct catacatgca ccagcgtgcc
     2821 ttcgtcttca ctcaggacca cgcgcctgag cgcgcccggt ttgacgatgg aaaatacctt
     2881 gaccggcaat ttttgatcgc ggcacagcgc aaaggcggtg gcatccatga cctgcagatg
     2941 cttggcgatc gcttcatcga aactgatggt gctgtagcgc gtcgctgcag gatccttcct
     3001 gggatcggat gtatagacgc catcgacctt ggtggccttc agaacgatct cggcgccgat
     3061 ttcagaaccg cgcagcgccg ctgcggtatc agtggtgaaa aacggattgc cggtaccggc
     3121 ggcaaacacc acgattttgc cctcttccag gtattgcaag gccttgggac gcacataagg
     3181 ctcgacgatc tgctcgatgc cgatggccga catgacacgc gccgtcagac cagtctgacg
     3241 catggcgtcg gccagcgcga gagagttcat gacagtcgcc agcataccca tgtaatcggc
     3301 cgtggcgcgg tccatgcctt gcgcccccgg tgcgacgccg cggaaaatat tgccgccccc
     3361 gatcacgatg gccagttcga cgcccagccg actgacctcc gccacatcgg caaccatgcg
     3421 ctcgatagtg gcgcggttga tgccgtaagc atcgtcgccc atcagggctt cgccggaaag
     3481 tttgaggagt acacgcttat aggctggtgt cgtcatgtgc tgggatccat ttgctatccg
     3541 attaactgat tctgcgcgcc ctgcggcgct ctgctgcgtt gcctatcctc gccgggccac
     3601 cagcccgcct gtgccccatc ctgaaagaac gatagtacga ctcccgacga aattctgaac
     3661 aggttcttaa aaaaacgggc ctcccggccc gcttgctgct tctttactgc gcctgcctgg
     3721 cagcggcaac ctgggctgcc acctcggctg cgaagtcgtc ctgttttttc tcgatgccct
     3781 caccgaccac aaacagagta aaccccttga gtacggtatt ggccgctttc aacatctgtt
     3841 cgacagtctg cttgtcgttc ttcacgaaag gctgatcgaa caacgacact tccttcagat
     3901 atttccgcac tgcgccatcg accatcctgg caacgatgtc gtccggcttg ccggattcag
     3961 ccgctttcag cgaagcaacc gaacgctctt tttcgatcag ggcggcaggc accaggtccg
     4021 acgacaaggc aaccggcttc atcgctgcga tatgcatggc gacatccctg ccgacctgtt
     4081 cgtcggcgcc atcgaaatcg accaccacgc cgatacgtgt gccgtgcaga taagaaacca
     4141 gcctgccggc tgtttcgaaa cgcacgaaac ggcggatcga catgttttca ccaatacggc
     4201 cgaccagctc ggcacgcttt tcttccaccg t
//
LOCUS       DASRDH010000130         2766 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_530961,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000130 DASRDH010000000
VERSION     DASRDH010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2766
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530961"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..919
                     /gene="fliD"
                     /locus_tag="VFA14_02990"
     CDS             <1..919
                     /gene="fliD"
                     /locus_tag="VFA14_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007882567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HZS80380.1"
                     /translation="TADITNDGTNDHLVLIHSGTSVTSPVSLNGTGDFSILSGSIMGQ
                     LQAAQDAKLSIDGISITSATNNVANAISGVTLNLNKVTTDADNMTLNITNDTSGVISA
                     ANTFVSAYNTLLKTITGLTQQTPSTIPGQPGSSSPLANEFAVQNIMAQLRKALVRSVQ
                     GGNGISTLTDIGIGFQKDGTLGLDAAKLSTATTNNFTGIANLFLSTGGSNADGAPNTT
                     GTDGVLKNLQTLVNKFLADGGIIDIKTKGLQASLKINTDRQMALQVRLENLQDQYTNQ
                     FNALNVTLANMTATQNYLMQQLEKLPKIN"
     gene            948..1367
                     /gene="fliS"
                     /locus_tag="VFA14_02995"
     CDS             948..1367
                     /gene="fliS"
                     /locus_tag="VFA14_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HZS80381.1"
                     /translation="MFGSTQLGANAYANIGVETGVAAVSPHKLITMLFDGALIAISMG
                     EKYMKAGDIKKKGESITQAILIIEGGLRTSLDKNAGGEIASNLDALYEYMGQRLLVAN
                     VENSTSILKEVHDLLDDLRDAWRTIGLDGADPMTTAA"
     gene            1408..1740
                     /locus_tag="VFA14_03000"
     CDS             1408..1740
                     /locus_tag="VFA14_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007882569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HZS80382.1"
                     /translation="MDATEVISLYESVAELTDQMLAAARREEWQLLSKLEMDCAHIVA
                     MLKKSEASVMLTDDRHERKIEILKKILEDSNDIRNLADPGLKKLSALIQNTSVNRKLV
                     KAYGISTR"
     gene            1750..>2766
                     /locus_tag="VFA14_03005"
     CDS             1750..>2766
                     /locus_tag="VFA14_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007882570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HZS80383.1"
                     /translation="MIPRADIANTVQPVATVDAPTSIVSVTDAKQEAFSRLAQIAIGQ
                     QLQGKVLSTYNDGSHLVRIANTVARMVLPAATKTGDSLLLTMISKEPRPTFLLTESGV
                     KKESSNASSSLSVRMMEQDLQLLKSASQGLQTVVGRNLPTLLVYILTASQGQQTVVGR
                     HAQLAEAKADAKTPQQGPGAAPASSQTSLSAAGKLIDTLLHTSEKSYLPGTIKADTPL
                     VDKPSNAVQLARVLHDTIALSGAFYEAHVAAWSEGKRPLSELQKEPQAQLTQQAQANS
                     SQNLLKSSTPSHTPLGQIIHLQINALEQQRIVWHGEAWPGQQMEWEISQDSSEAPQEE
                     KQESN"
BASE COUNT          796 a          783 c          682 g          505 t
ORIGIN      
        1 gactgccgac atcaccaacg acggcaccaa cgatcacctg gtactgatcc attccggcac
       61 cagcgtgacc agccctgtct cgttgaacgg cacaggtgat ttctcgatac tgagcggcag
      121 catcatgggc caattgcaag ctgcccaaga tgccaaactg agtatcgatg gtatcagtat
      181 caccagcgcc acaaacaatg tcgccaatgc gatttccggt gtgacgctga acctgaacaa
      241 ggtcacgacc gacgccgaca acatgacgct aaacattacc aacgacacca gcggcgtcat
      301 cagcgcagca aacaccttcg tttcggcata caacacactg ctcaaaacga tcactggcct
      361 gacccagcaa acgccatcga cgatacccgg ccaacccggg agctcctccc cattggcaaa
      421 cgagttcgcc gtacaaaaca tcatggcgca attgcgtaaa gcccttgtac gcagtgtgca
      481 aggcggcaat ggtatcagca ccttgacaga tatcggtatc ggcttccaga aagatggcac
      541 gctgggcctg gatgcggcga aactgtcgac ggcaacgacc aataatttta ccggtattgc
      601 aaacctgttc ttgtccacag gcggcagcaa tgccgatggc gctcccaata caacgggtac
      661 agacggcgta ttgaagaatt tacagacgct ggtcaataaa tttctagcag acggcggtat
      721 cattgacatc aagaccaaag gtttgcaagc tagcctgaaa atcaataccg accgtcagat
      781 ggccctccag gtgcgtttgg aaaacttgca agaccagtat acgaatcaat tcaatgctct
      841 gaatgtaacg ctggccaaca tgacagcaac gcaaaattac ttgatgcagc aactggaaaa
      901 gttaccgaaa atcaactaat tgccatgatc gaacacagga gacgcgaatg ttcggaagca
      961 cgcagcttgg agcaaatgcc tacgcaaata ttggtgtaga gaccggtgtt gccgcagtaa
     1021 gtccgcacaa actgatcacc atgctgtttg atggcgccct gatagcgatc tccatgggtg
     1081 aaaaatatat gaaagccggt gatatcaaga aaaaaggcga atcaatcacc caggcaattc
     1141 tcatcatcga ggggggcctg cgcaccagcc tggacaagaa tgccggcggt gagatcgctt
     1201 cgaatctgga tgcgctttac gaatatatgg gacaacgctt gctggttgcc aatgtcgaaa
     1261 attcgacaag cattctgaaa gaagtccacg acctgctgga cgacctgaga gatgcctggc
     1321 gaacaattgg tctagatggc gccgatccga tgacaacagc cgcctgagcc ggcacccggt
     1381 ctgacaaatg tcagggaaag tacaagcatg gacgctacag aagtcatctc tctatatgaa
     1441 tcggttgccg agctgactga tcagatgctg gcagctgcgc gccgggaaga gtggcagttg
     1501 ctctcaaaac tcgaaatgga ttgcgcgcat atcgtcgcca tgttgaaaaa gagtgaagcc
     1561 agcgtcatgc taaccgatga ccgtcatgaa cgtaaaatcg aaattttgaa gaaaattcta
     1621 gaagatagca acgatattcg caacttagcc gaccccggcc tgaaaaaact ttccgccctg
     1681 atccagaaca ccagcgtcaa tcgcaagctg gtcaaagcct atggcatatc cacaaggtga
     1741 gtgagagtga tgataccgcg ggccgacatc gccaacacgg tccagcccgt cgctaccgtc
     1801 gatgcgccga cctctatcgt ttcggtcacc gacgcaaaac aggaggcttt cagccgtctg
     1861 gcgcaaattg ccatcggaca acaattacaa ggcaaagtac tgtccaccta caacgatggt
     1921 agccatctgg ttcgcattgc caatacggtc gcgcggatgg tgctgccggc tgcaaccaaa
     1981 accggcgact ccctactgct gacgatgatc agcaaagaac cgcgcccgac tttcctgctg
     2041 accgaaagcg gcgtaaaaaa agaaagctcc aacgcctcct ccagtctctc cgtccgaatg
     2101 atggagcaag acctgcaatt actgaaatca gcgtcacaag gtctgcaaac cgtagtcggg
     2161 cgcaatctgc cgaccttgct agtgtatatc ctcacagcat cacaaggtca acaaaccgtg
     2221 gtcgggcgcc atgcgcagct ggccgaagcc aaggctgacg ccaaaacgcc acagcaagga
     2281 ccgggcgcag ccccggcaag tagccagaca tcccttagtg cagccggaaa actaatcgat
     2341 accctgttgc atacgtctga aaaaagctat ttacccggca ctatcaaagc tgatacacca
     2401 ctggtcgaca aacccagcaa cgccgtccaa ctcgccagag tactgcacga caccatcgcc
     2461 ctgagcggcg ccttctatga agcgcacgtc gctgcatgga gcgaaggcaa acggccgctc
     2521 tccgagcttc agaaagagcc acaggcacaa ctgacccagc aagcacaggc aaacagcagc
     2581 cagaatttac tcaaaagcag cactccctcc cataccccgc tcggtcagat cattcatctt
     2641 cagatcaatg ccctggagca acaacgcatc gtctggcacg gagaagcatg gccaggacag
     2701 cagatggagt gggaaattag tcaggattca tccgaagcgc cgcaggaaga aaagcaggaa
     2761 agcaac
//
LOCUS       DASRDH010000131         2181 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1542564,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000131 DASRDH010000000
VERSION     DASRDH010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1542564"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            305..1507
                     /gene="earP"
                     /locus_tag="VFA14_03010"
     CDS             305..1507
                     /gene="earP"
                     /locus_tag="VFA14_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017876499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0106361 - protein-arginine
                     rhamnosyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HZS80384.1"
                     /translation="MHPTRSSATAAKPAALALFCKVVDNYGDIGICWRLSRQLQREYG
                     IAVTLWVDDLDRFQRICPEGASDRQTQEIQGIMIRHWRDQEGRFDAAEIPDVVIEFFG
                     CAIPPDYIEAMSRCMPRPVWLNLEGLSAESWVESCHTLPSPHTRLPLTKYFFFPGFTP
                     RTGGLQHEAGLDVQRSVFQKDPAAMRDFLSRFGVTTDEMAALKVSLFCYPQAPIQDLL
                     DTWRHADKPVLCLVPEGVAAAAISAFLGQTASAGANATHGALTVRVLPFMPQPDYDKL
                     LWACDLNFVRGEDSFVRAQWACKPFIWHIYSQDENLHHIKLKAFLGNYGAATGSLVAL
                     TLAWNGVTQNAVKTLWPAFAADMPDITRLSMDWQARLLANGDLVSNLLQFAQTVQQSA
                     RENGPNMG"
     gene            1544..2119
                     /locus_tag="VFA14_03015"
     CDS             1544..2119
                     /locus_tag="VFA14_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HZS80385.1"
                     /translation="MKFAKEIRVGNIIMVDSKPMIVLRSDVNGSSRTGFTYKWKMKNL
                     LTNSPQENVFRGDDKFDVVILDKKEVTYSYFADPLYVFMDAEYNQYEIEGENLGDALG
                     YLKDGMECEAVFYDGKAISVELPTTIVRQVVYSEPAVKGNTSGNVLKEARIENAIEAN
                     RLTVMVPLFVSQDDMIEIDTRTNEYKKVVRS"
BASE COUNT          520 a          598 c          583 g          480 t
ORIGIN      
        1 tggtatctct cctgaagatg aatacaacgt aacggcgacg cgatcacccg gtttctccca
       61 tcatgatgaa acgtgtccgc caaggcggtg agatcgtcga aacacgaatg ccaatcgccg
      121 ctgaaaggcc agagacatag ctttttggca acaatctcag cactttgaga tgtcgtgagt
      181 taaaaagttc acaaaaattt tgtaacaatg aaacgagcca gcggatttta caccacatcc
      241 ggctcctcct cttccggcaa gcgcgacccg cttcgggtct ccggtattcc tgtatcatgc
      301 cgccatgcat ccgacgcgct cttccgcaac cgcagcgaag cccgccgcac tcgccctgtt
      361 ctgcaaagta gtcgataact atggtgacat cggtatttgc tggcggctgt cgcggcaatt
      421 gcaaagggaa tatggtatcg ccgtcacctt gtgggtcgat gatctcgacc gttttcagcg
      481 gatttgccct gaaggagcaa gcgatcgcca gacgcaggaa atccagggca tcatgatcag
      541 acattggcgc gaccaggaag gtcgattcga cgccgcagag attcccgacg tggtcatcga
      601 gtttttcggt tgtgctatcc cgccggacta cattgaagcc atgtcccggt gcatgccgcg
      661 tccggtctgg ttgaatctgg aaggactcag cgctgaaagc tgggtcgaaa gctgtcatac
      721 actgccatcg ccgcataccc ggttaccgct gacaaaatat ttcttctttc cgggattcac
      781 gccacgcacc ggtggcctgc aacacgaagc cggactggat gtgcagcgca gcgtttttca
      841 gaaggatccg gcggcgatgc gggattttct cagccggttc ggcgtgacca cggatgaaat
      901 ggcagcgctc aaagtgtcac tattctgcta tccacaggcg ccgatccagg acttgctgga
      961 tacctggcgg catgctgata aaccggtgct gtgcctcgtt cccgaaggcg tggcagcagc
     1021 agccatcagc gcctttctcg gccagacggc cagtgcaggc gcgaacgcca cacatggtgc
     1081 gctgaccgtg cgggtactgc cattcatgcc acaaccggac tacgacaaat tgttgtgggc
     1141 ctgcgatctc aacttcgtgc gcggggaaga ttctttcgtg cgcgcccaat gggcctgcaa
     1201 gccattcatc tggcatattt attcgcagga tgaaaatctg caccatatca agctcaaggc
     1261 cttcctcggc aactatggcg ccgctactgg cagtctggtc gcgctgacat tggcatggaa
     1321 cggcgtaaca cagaacgccg taaaaacctt gtggccggct tttgctgccg acatgcccga
     1381 cattaccagg ctatccatgg attggcaggc gcgattgttg gcaaatggcg acctggtcag
     1441 caacctgctg cagtttgcgc aaacagttca gcaatcggcc agggaaaacg gtccgaatat
     1501 gggttaaaat gtgaggcttg tttttattca ttgatgattc gccatgaaat ttgcaaaaga
     1561 aattcgcgtc ggcaacatca ttatggtcga cagtaaaccg atgatcgtcc tgcgctccga
     1621 tgtcaatggc tccagccgca ccggcttcac ttacaagtgg aagatgaaga acctgctgac
     1681 caacagcccg caggaaaatg tcttccgggg cgatgacaag ttcgacgtgg tcatactgga
     1741 caagaaggaa gtcacttact cctacttcgc cgatccgtta tatgtattca tggacgccga
     1801 gtacaaccag tacgaaatcg aaggcgaaaa tctgggcgat gcgctcggtt acctgaaaga
     1861 cggcatggag tgcgaagcgg tcttttacga tggcaaagcc atctcggtcg aactgccgac
     1921 taccatcgtg cgtcaggtcg tgtactccga gccagccgtc aagggcaata cttccggcaa
     1981 tgttctgaaa gaagccagga tcgaaaatgc catcgaagca aaccgtctga ccgtgatggt
     2041 cccgctgttc gtcagccagg atgacatgat tgaaatcgac acacgcacca acgaatacaa
     2101 gaaagtcgtg cgcagttgat tacctgctca tcggctgcac aagcaaaaag cgctgcaatt
     2161 ttccgcagcg tttttcgtct g
//
LOCUS       DASRDH010000132         2466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_181097,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000132 DASRDH010000000
VERSION     DASRDH010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181097"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="VFA14_03020"
     CDS             <1..182
                     /locus_tag="VFA14_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007882599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar hook-associated protein 3"
                     /protein_id="HZS80386.1"
                     /translation="AQGYILNEQYTKIGNDLLGRDISDTIDLLSQLTLQQQYLQTAQK
                     VFASTSNLTLLNYLR"
     gene            complement(300..983)
                     /locus_tag="VFA14_03025"
     CDS             complement(300..983)
                     /locus_tag="VFA14_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HZS80387.1"
                     /translation="MHRHRRKRLQKLIADRYARSRRRFCDASGLSESRLAQLLSATYR
                     DGQGFGEKAARALEKRLGLSELYFDRLSSGEINPPYSWQQALAADAVAIPQVSLTLNA
                     GTPHIGMQETFPTPCALLFPTHWIETHDLLPEKLFATAVTDASMSPLLQESDVVLVDS
                     NDTALQDNMVYLFNFHGKAIIKRLAMDFGQWFMTSDNASAKRYPRQQFDQPACIVIGR
                     IILRVSAHI"
     gene            complement(1105..1800)
                     /locus_tag="VFA14_03030"
     CDS             complement(1105..1800)
                     /locus_tag="VFA14_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018575639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein Fic"
                     /protein_id="HZS80388.1"
                     /translation="MLEGNPFTFPEVKTLLDGITIGGRKISDQEQVLNLAESSRRLLA
                     MVRSGRFALSKSVFTELNGIVARQEALEWGLFRGEGQEKNYTPDVGLGECGRYTPLPT
                     LPDAPELNRVFREGVTALQKCEPFERATAFFLFGALQQFFFDGNKRTSRFMMNGVLMS
                     QGIDAISIPAAKAQEFNEKMVRFYLSKDATGMMAFLAGCHPEAKAILPSGEDHDSEVA
                     PQSWCGTDISTPD"
     gene            complement(2128..>2466)
                     /locus_tag="VFA14_03035"
     CDS             complement(2128..>2466)
                     /locus_tag="VFA14_03035"
                     /inference="COORDINATES: protein motif:HMM:NF012920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HZS80389.1"
                     /translation="PCALLFPTHWIETHDLLPEKLFATAVTDSSMSPLLQESDVVVVD
                     SNDTALQDNMVYLFNFHGKATIKRLAMDFGQWFMTSDNTSAKRYLRQQFNQPACIVIG
                     RIILRVSARI"
BASE COUNT          539 a          618 c          730 g          579 t
ORIGIN      
        1 atgcgcaagg ctacatattg aatgagcaat ataccaagat tggcaacgat ctgcttggcc
       61 gtgacatcag cgacaccatt gatttgctga gtcaactgac cctgcagcag caatacttgc
      121 agacagcgca aaaagtgttt gcgtcgacca gcaatctgac gttgctgaat tatctgagat
      181 aagtttttta ccggatggtt tgtccgtccg gtatccaggg ggcattgcgt ggcggtcaat
      241 cgcgcccaga aacatgctgt gattgaagag ggggagcgca gtgcgtgatt ggcgctgcgt
      301 cagatgtgcg cgctgacgcg caggatgatg cgcccgatca cgatgcaggc aggctggtcg
      361 aactgctggc gcggatagcg tttggcgctg gcattatccg aggtcatgaa ccattgtccg
      421 aagtccatgg ccagtcgttt gatgatggct ttgccgtgaa aattgaagag atagaccata
      481 ttgtcctgca aggcggtatc gttggaatcg accaagacga catcactctc ctggagcagc
      541 ggtgacatgc tggcatctgt gacggcagtg gcaaacagtt tttcaggaag caggtcgtgt
      601 gtctcgatcc agtgcgttgg aaacagcagg gcgcatggtg tggggaaagt ttcctgcatg
      661 ccgatgtggg gcgtgccggc gttcagcgtc aggctgacct gagggatggc gacggcatcc
      721 gcggccaatg cctgctgcca ggaatagggc gggttgattt cgccgctcga gagccgatcg
      781 aaatacagct cgctgaggcc gagccgcttt tccagggcgc gcgcagcttt ttcgccgaat
      841 ccctgtccat cccgataggt ggcagagagt agttgcgcga ggcgggattc gctcaggccg
      901 ctggcatcgc agaagcgcct gcggctgcgg gcatagcgat ccgcgatcaa tttctgaaga
      961 cgtttgcgtc gatgtcggtg catgctcata gcgctattta agcgcatatt tagcatttgg
     1021 taaattatct actgcttgaa ttttaagcag ttattgctaa attggcgaca tgagaatgat
     1081 gaaacgcaag gtgcaagcca caggctaatc aggtgtcgaa atgtccgtgc cgcaccatga
     1141 ttgcggcgcc acttcgctgt catgatcttc cccggatggc aagatcgcct tggcttccgg
     1201 gtgacacccg gcaaggaatg ccatcatccc ggttgcgtcc ttcgagagat agaagcgaac
     1261 cattttttcg ttgaattctt gcgccttggc ggctggaatg ctgatggcgt cgatgccttg
     1321 cgacatgagg acaccgttca tcatgaagcg cgacgtgcgc ttgttgccgt cgaaaaagaa
     1381 ctgctgcaat gcgccgaata ggaagaaagc ggtagcccgc tcgaacggct cgcatttttg
     1441 cagtgcggtc acaccctcgc ggaatactcg gttcagttcc ggtgcgtcag gcagtgtcgg
     1501 tagtggggta tagcgcccgc actccccgag tcctacgtcc ggggtgtagt tcttctcctg
     1561 accttcgccg cggaacaggc cccattccag ggcttcctga cgtgcgacga tgccgttcag
     1621 ttctgtgaac accgactttg aaagcgcgaa ccggccactc ctgaccatgg ccagcagtcg
     1681 cctggagctt tctgccagat tcaggacttg ctcctgatcg gaaattttcc gaccgccgat
     1741 ggtgatgccg tccagcagtg tcttgacctc cgggaaggta aatgggttgc cttccagcat
     1801 tgatgcgtcc cacacgaatt caggcagcat tttatggaag cggaagcaca ctcgttcgat
     1861 agaatgaacc ggaacggaca ccgccgatct gtcccatcgg aatccgagaa catcaaataa
     1921 catcatgtca ccacctcatc gatcattcaa cacgggttcg tggtacatgc tatctgaaaa
     1981 gctccgtagc atatcagttc tccttgatat tgaaatcctt gtttttccga ggaggagggg
     2041 aaagggtttt gtcctattct gctcgggttc aaggggtttt taagagaaga ggagacattg
     2101 gcagtgtacg gtacgcgtac ttgcctctta tatgcgcgcg ctgacgcgca ggatgatgcg
     2161 tccgatcacg atacaggccg gctggttgaa ttgctggcgc agatagcgtt tggcgctggt
     2221 attatccgag gtcatgaacc attgtccgaa gtccatggcc agccgtttga tggtggcctt
     2281 gccgtgaaaa ttgaagagat agaccatatt gtcctgcaaa gcggtatcgt tggaatcgac
     2341 cacgacgaca tcgctctcct gaagcagtgg cgacatgctg gagtcagtga cggcagtggc
     2401 aaacagtttt tcaggaagca ggtcgtgtgt ctcgatccag tgcgttggaa acagcagggc
     2461 gcatgg
//
LOCUS       DASRDH010000133         3712 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1505160,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000133 DASRDH010000000
VERSION     DASRDH010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 3712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3712
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1505160"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1483
                     /locus_tag="VFA14_03040"
     CDS             <1..1483
                     /locus_tag="VFA14_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017449793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HZS80390.1"
                     /translation="DPLPCPSDWSFELIERYNDEIANVAASYGLDTHPVQLEIISAEQ
                     MMDAYASSGMPVNYRHWSFGKHFLATERSYKRGQMGLAYEIVINSNPCIAYLMEENTM
                     TMQALVVAHAAYGHNSFFKGNYLFQLWTDASAIVDYLIYARSYIAECEERHGFDRVEE
                     LLDSCHALMNYGVDRYKRPQRMSLAQERAQQREREAYAQSQVNELWRTLPSRKEAAAT
                     QTEERFPSEPQENLLYFAEKNAPLLEPWKREIIRIVRKIGQYFYPQRQTQVMNEGWAT
                     FWHYTLLNTLYDNGRLTDGFMMEFLHSHSNVVFQPPITEPYYNGINPYALGFSMMSDI
                     RRICENPTDEDRQWFPDVAGSNWLDTLHYVMRNFKDESFIAQYLSPRLMRDMRMFAVL
                     DDENCSELEVSAIHNQSGYQHVRQVMSRQYDINHREPNIQVWSVDTRGNRSLTLRHFR
                     SDGRTLGDNTDEMLRHMARLWQFNVHLESVDDNGHIVQRYECN"
     gene            complement(1573..2154)
                     /locus_tag="VFA14_03045"
     CDS             complement(1573..2154)
                     /locus_tag="VFA14_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HZS80391.1"
                     /translation="MKRLLFLLPAVLLGCLLTACQQADTFINTDMTGREVARDFALTD
                     HNGTPRALADFRGKAVVMFFGYTQCPDVCPTTMTEMANVMTELGPQADKVQVLFVTID
                     PARDTPQILAQYAPAFDRRFLGLYGDDAATERIAREFKVFLQKVPGKTADSYTMDHTA
                     GSYVFDPQGCIRLFIRHGQDAQSIVHDLKLLLS"
     gene            complement(2154..3047)
                     /gene="cyoE"
                     /locus_tag="VFA14_03050"
     CDS             complement(2154..3047)
                     /gene="cyoE"
                     /locus_tag="VFA14_03050"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HZS80392.1"
                     /translation="MATLTVQTNRMAQYWALTKPRVTQLAVFCAVIGMLLATPDLPDW
                     RRVGFAAIGIWLLAGAAFAINCLVEREIDSRMARTARRPMARGEITVTQTLIFSSVIG
                     GMGMWVLYSFVNALTMWLTLATFVGYAVIYTIILKPLTPQNIVIGGLSGAMPPALGWA
                     AITNDVPMQAWILVLIIFIWTPPHFWALALYRRDDYIKSGLPMLPVTHGMQFTQFHIL
                     LYTIALMSATMLPFAVGMSGLIYLVSAAALGLVFFYYAWQVYRHYTNRIAYRTFSYSI
                     VYLSLLFAALLADHYLLFRTF"
     gene            3355..>3712
                     /gene="hslV"
                     /locus_tag="VFA14_03055"
     CDS             3355..>3712
                     /gene="hslV"
                     /locus_tag="VFA14_03055"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020653769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HZS80393.1"
                     /translation="MEQFHGTTILCVRRGSTVALGGDGQVTLGNVVMKGTARKVRKLY
                     DGKVLVGFAGGTADAFTLLDLFESKLDKHQGNLMRASVELAKEWRTDRMLRRLEAMLL
                     TADRDSTLVITGNGDVL"
BASE COUNT          862 a         1038 c         1048 g          764 t
ORIGIN      
        1 cgatcccctg ccttgcccgt cagactggag cttcgagttg atcgagcgtt acaacgacga
       61 gatcgccaac gtcgcggcaa gctatgggct ggatacccat ccggttcagc tggagatcat
      121 ttcggcggaa cagatgatgg atgcctatgc ctcctcaggc atgccggtca attatcgtca
      181 ctggtcgttc ggcaagcatt tcctggctac cgaacgcagc tacaaacgcg gccagatggg
      241 gctggcttac gaaatcgtga tcaattccaa tccttgcatc gcctatctga tggaagaaaa
      301 cacaatgacg atgcaggcgc tggtggtggc gcatgcggca tatggacaca actctttctt
      361 caaggggaac tatctgttcc agctatggac cgacgccagc gccatcgtcg attatctgat
      421 ctacgccagg agttatatcg ctgaatgcga agaacggcat ggcttcgatc gtgtcgagga
      481 attactcgac tcctgccacg cactgatgaa ttacggcgtc gaccgctata agcggccgca
      541 gcggatgtca ctggcgcagg aacgcgcaca gcagcgcgaa cgagaagcct acgcgcaatc
      601 gcaggtcaac gagttgtggc gtaccttgcc gtccaggaaa gaagcggcgg caactcagac
      661 ggaagaacgc ttcccgtcgg agccgcagga aaatctgctg tatttcgccg aaaagaacgc
      721 ccctttgctg gagccatgga agcgcgaaat catccgtatc gtgcgcaaga tcggccaata
      781 tttttatccg cagcggcaaa cccaggtcat gaacgaaggc tgggcgacat tctggcatta
      841 caccctgctc aacaccctgt atgacaatgg caggctgacc gatggcttca tgatggaatt
      901 cctgcattcg cacagcaatg tcgtattcca gccaccgatc accgaaccgt attacaacgg
      961 catcaatccg tatgcgctgg gattttcgat gatgagcgac atccgccgca tctgcgaaaa
     1021 tcccaccgac gaggataggc aatggtttcc cgacgtggcc ggcagtaact ggctcgatac
     1081 gctgcactat gtcatgcgta acttcaagga cgaaagcttc atcgcgcaat acctgtcacc
     1141 caggttgatg cgcgacatgc gcatgttcgc cgtgctcgac gatgaaaatt gcagcgagct
     1201 ggaagtttcg gccatccaca atcagagcgg ttatcagcat gtacgccaag taatgtcacg
     1261 ccagtacgac atcaatcatc gcgaaccgaa cattcaggta tggtcggtcg atacgcgcgg
     1321 caatcgcagc ctgaccttgc gtcatttccg cagtgacggt cgtacgctcg gcgacaatac
     1381 tgatgagatg ctgcgccata tggcgcgact atggcaattc aatgtacacc tggaaagcgt
     1441 cgacgataac ggccacatcg tgcagcgtta tgaatgcaat tgaggggtag cgtacaggcg
     1501 ccgatacttc tatcttcacc tatcatcgtt ttctcagaac gtcttcactg gatactgacg
     1561 agagcgtcgc gatcaggaca acagcagttt cagatcatgc acgatagact gcgcgtcctg
     1621 tccatgcctg atgaacaggc ggatgcatcc ctgtggatcg aatacatagc tgccggcggt
     1681 gtggtccatg gtgtaactgt ctgccgtctt gcccggcact ttctgtaaaa ataccttgaa
     1741 ttccctggcg atcctttctg tggccgcatc atcgccgtat agacccagga agcgtctgtc
     1801 gaaggctggg gcatactgcg ccaaaatctg cggtgtatcg cgcgccggat cgatggtcac
     1861 gaacagcacc tgcaccttat cggcctgcgg tccgagttcc gtcatcacat tggccatttc
     1921 cgtcatcgtg gtcgggcaaa catcgggaca ttgcgtataa ccgaaaaaca tcaccaccgc
     1981 cttacccctg aaatcggcca gcgcgcgcgg tgtgccgttg tggtcggtca gcgcgaaatc
     2041 tctggcgact tcccggccgg tcatatcggt gttgatgaat gtgtctgcct gctgacaagc
     2101 tgtcagcagg catcccagca agactgccgg cagcaggaat aataaacgtt tcatcagaaa
     2161 gtcctgaaca gcaaatagtg atcggctagc agcgcagcga acagcaggga cagataaacg
     2221 atcgagtagg aaaatgtcct gtacgcaatc cggttcgtat agtgacgata gacctgccag
     2281 gcgtagtaga aaaaaaccag tcccagcgcc gccgcgctga ccagatagat caggccgctc
     2341 atgcccaccg caaagggcag catggtagcc gacatcagcg cgatcgtgta cagcaggata
     2401 tggaattgcg tgaactgcat gccatgtgtg accggcagca tcggcagacc ggacttgatg
     2461 tagtcgtcac ggcgatacag agccaacgcc cagaaatgcg gtggcgtcca gataaaaatg
     2521 atcagcacca gaatccacgc ctgcatcggt acgtcgttgg tgatggctgc ccagcccaac
     2581 gccgggggca tcgcgcccga gagaccgccg atgacgatgt tctgcggtgt cagtggcttg
     2641 aggatgatgg tatagatgac ggcatagccg acgaaggtcg ccagcgtcag ccacatggtc
     2701 agcgcattga cgaagctgta tagcacccac atacccatgc cgccgatcac gctggaaaat
     2761 atcaatgttt gcgtcacggt gatttcgccg cgcgccatcg gacggcgtgc agtgcgggcc
     2821 atgcgtgaat cgatctcgcg ctcgaccagg cagttgatgg caaaggcagc gccggcaagc
     2881 agccagatac caatcgcggc gaatccgacc ctgcgccagt cgggcagatc cggcgtggcc
     2941 agcaacatac cgatgacggc gcaaaaaacc gcgagctggg tcacgcgcgg cttggtcagc
     3001 gcccagtact gagccattcg gttcgtctgt acggtcaaag tggccatgtc agagtctatt
     3061 cacaatcagt ttcctggcgc cgcagctcct gatgatcttg cgtaactgca tgcgcatgct
     3121 ctcaatggcg ttggtagtgt agatgatttt acacacctcg gggggagacg caaagaacgg
     3181 aatcgcccgc ttccactgag gtttgccgca tggtcgccat agtcagggat tgtctgtcct
     3241 ggccgcctgt gcgacttatt cacaggtggc ttgcaatcca tgcgcttgtc cccatcttgc
     3301 caaaaacggt aaagtcgcat ttcgtagaaa aataccattt attgaggttt taatatggaa
     3361 cagtttcacg gtacgaccat cctttgcgtg cgccgcggta gtaccgtggc gctgggtggc
     3421 gacggccagg tgacgctcgg caacgtcgtc atgaagggca cggcgcgcaa ggtgcgtaaa
     3481 ctctatgatg gcaaggtgct ggtcggcttc gccggtggca cggcagatgc ctttacgttg
     3541 ctcgatctgt tcgagtccaa gctggacaaa caccagggca acctcatgcg cgcctcggtc
     3601 gagctggcca aggagtggcg caccgaccgc atgctgcgcc gcctcgaagc catgctgttg
     3661 acggcggacc gtgacagcac gctggtgatt accggcaatg gcgacgtgct gg
//
LOCUS       DASRDH010000134         2541 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1534252,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000134 DASRDH010000000
VERSION     DASRDH010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1534252"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="VFA14_03060"
     CDS             complement(<1..376)
                     /locus_tag="VFA14_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadB"
                     /protein_id="HZS80394.1"
                     /translation="MMVAPALWLTAAAMLLLAIGILLWQNARALRHRQAASIFVDQQI
                     RKLVPQPEAETAGVADRPVFLALLSSFRPWRHFFLRAGVTPGVRLYIRLLLPALALAT
                     LATIFGGVLAMIGILSMYIVLVY"
     gene            complement(373..1692)
                     /locus_tag="VFA14_03065"
     CDS             complement(373..1692)
                     /locus_tag="VFA14_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HZS80395.1"
                     /translation="MSDDIELAGGNEAFVNTQRFQDIKAAAYDNLLTRIEELGAEFGR
                     WSRSAIQQFVNLDVDGFVRLRRIPLNESEVRLIADALTKELAGLGPLEDLLNDPTVED
                     ILINGYNDVFVSRHGVLERETLRFTDNNHLLRIIRRILAPLGRRLDESNPMVDARLPD
                     GGRLNVVIEPLAVDGPMVSIRKFRKEPLKPADLLQLGTFNEQLFALLEQAVKARCNIL
                     ISGGTSSGKTSLLNALAFFVPVNERVVTIEDTAELSLSHPHVVRLESRVGSFEGTGVV
                     TIRDLIRNSLRMRPDRIVVGEVRGAEVLEMLQAMNTGHDGSMGTIHANTPRECLHRIE
                     MLAGFAGFQGSESSLRRQIAGAIDFIVQIARLSNGLRRIVSITEITGVSDNIITMQEL
                     YRHEIRTAPNGEDMDCWVSLGIPPHSPKLKKRSQSQMPATGNPPRRE"
     gene            complement(1705..>2541)
                     /locus_tag="VFA14_03070"
     CDS             complement(1705..>2541)
                     /locus_tag="VFA14_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CpaE"
                     /protein_id="HZS80396.1"
                     /translation="QHGSLIALLGARAGIGTTTLAVHLADFLRRQKIAGRDECRVALL
                     DLGWPAGDGQLYLNVSGHFHFTDVVNNRQRLDQTLIQTALAHSQSGIGVISLPRALRW
                     MDDLNDADMVVALTLLRSYFDVLVMDLGGLPDPALAIRMAAAADKTWLLSDQSTGSLV
                     SLAALIAESDRQGAQRMAERRLIVNRYDKQYGMSAEQIAGRFQLELIAAIPDRTAALM
                     DSANQGKLLHQVAGNDPYLEALAKLAEEIPGVTVKRSGIGALSSWLSGTLFEKQRAAW
                     QK"
BASE COUNT          540 a          789 c          713 g          499 t
ORIGIN      
        1 aatagaccaa cacgatatac atcgacagga tgcctatcat ggccagaacg ccaccgaaga
       61 tggtggccag cgtcgccagc gccagcgccg gcagcagcag gcggatatac aggcggactc
      121 ccggcgtgac gccggcgcgc agaaagaaat gacgccacgg gcgaaagctg ctgagcaacg
      181 ccagaaatac cggccgatcc gccacgcccg ccgtttccgc ttcaggctgc ggcaccagct
      241 tgcgtatttg ctgatcgaca aatatcgaag cggcttgccg atgccgcaat gctctggcgt
      301 tctgccacag caggatgccg attgccagca gcagcatggc tgccgccgtc agccataatg
      361 ccggcgcgac catcattctc tccgtggcgg attgccagta gccggcattt gcgactggct
      421 ccgctttttc agtttcggcg aatgcggcgg tatgcccagc gacacccagc agtccatgtc
      481 ttcgccattg ggcgcggtgc ggatttcgtg gcgatacagc tcctgcatag tgatgatgtt
      541 gtcgctgacg ccggtgattt cggtaatcga cacgatacgc cgcaaaccgt tggaaagacg
      601 ggcgatctgc acgatgaagt cgatcgcgcc ggcaatctgc cgccgcagac tcgattcgct
      661 gccctgaaaa ccggcgaaac ccgccagcat ctctatgcgg tgcagacatt cgcgcggcgt
      721 attggcatgg atggtaccca tcgacccatc gtgtccagta ttcatggctt gcagcatttc
      781 caggacttcc gcgccacgca cttcaccgac gacgatgcga tccggacgca tgcgcaggct
      841 gttgcggatc agatcgcgaa tagtcaccac gcccgtgcct tcaaagctac ctacgcgcga
      901 ctccaggcgc accacatgcg gatgacttag ggaaagttca gcggtgtctt cgatggtcac
      961 gacccgttca ttcacaggaa cgaagaaagc cagggcattg agcagcgaag tcttgccgga
     1021 actggtgccg ccggaaatca ggatattgca gcgcgctttg accgcctgct ccagcaaggc
     1081 aaacaactgc tcattgaagg tgccgagttg cagcaggtcg gctggtttca gcggctcttt
     1141 gcgaaacttg cggatcgata ccatcgggcc gtccaccgcc agcggttcga tcacgacatt
     1201 gagacggcca ccgtccggca aacgcgcatc gaccatggga ttcgactcat ccaggcggcg
     1261 tccgagcggc gccagaatgc ggcgaatgat gcgtaacaaa tggttattgt cggtgaagcg
     1321 cagggtttcc cgttccagca cgccatggcg ggaaacgaac acgtcgttat aaccgttgat
     1381 cagaatatcc tcgacagtag gatcattcag caggtcttcc agtggcccca gcccggccag
     1441 ctccttagtc aaggcatcgg caatcagccg gacttcgctt tcgttgagcg gaatgcgccg
     1501 cagacgaaca aaaccatcga catccagatt gacgaactgc tgtatcgccg aacgtgacca
     1561 gcggccgaat tccgcgccga gttcttcgat ccgcgtgaga aggttgtcat aggcagccgc
     1621 cttgatgtcc tgaaaccttt gcgtgttaac aaaagcttcg ttgccgcctg ccagttctat
     1681 gtcatcgctc atgtcagtgc ttccttattt ctgccatgcc gctcgctgtt tctcgaacag
     1741 ggtgccggac agccagctcg acaatgcgcc gatgccgctc cgtttcacag tcacgcccgg
     1801 aatttcctcc gccagtttcg ccagcgcttc caggtaaggg tcgttaccag cgacctggtg
     1861 cagcaacttg ccttgattgg cgctgtccat caacgctgct gtccggtccg gtattgctgc
     1921 aatcagttcc agctggaatc gcccggcaat ctgctcggca ctcattccgt actgcttgtc
     1981 gtaacggttg acgatcaggc gccgctctgc catgcgttga gcgccctgtc tgtcggattc
     2041 ggcaatcaat gccgccagtg acaccaggga acctgtactc tggtcggaca acaaccatgt
     2101 cttatccgcc gcggcagcca ttctgattgc cagcgccgga tccggtagac cgcccagatc
     2161 catcaccagc acatcaaaat acgaacgcag cagcgtcagc gccacgacca tatcggcatc
     2221 gttcaaatcg tccatccagc gcagggcgcg cggcagggaa atgacgccga tgccgctctg
     2281 actgtgcgcc agtgcggtct ggatcagggt ctggtccaga cgctgacgat tattgacgac
     2341 atcagtaaaa tggaaatgac cggaaacatt cagatacagt tgaccatcgc ccgccggcca
     2401 ccccagatcg agcagggcga cacggcattc gtcccgcccg gcgatctttt gccggcgcaa
     2461 aaaatcagcc agatgcaccg ccagagtggt cgtgccgatg ccggcgcgcg cacctagcag
     2521 ggcgatcagg ctgccatgct g
//
LOCUS       DASRDH010000135         3241 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_625265,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000135 DASRDH010000000
VERSION     DASRDH010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 3241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3241
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_625265"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..505)
                     /locus_tag="VFA14_03075"
     CDS             complement(<1..505)
                     /locus_tag="VFA14_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007879964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS80397.1"
                     /translation="MASLFDDTRMTAPTSIDADTPTPPMPRNNRRSGLLLGLLALLLI
                     LFMALVWAASTDSGTRSVWRAAVWLMQGKLSGTYAGGNLAGGLSLRDLRYRNAAMQLD
                     IDRIDARWSLSLIQRCLSVAYLRVGTVTIDKQATAPTPARMPTSLRFPLSLNLRDIAL
                     QKLTLRQG"
     gene            complement(489..2258)
                     /locus_tag="VFA14_03080"
     CDS             complement(489..2258)
                     /locus_tag="VFA14_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex family protein"
                     /protein_id="HZS80398.1"
                     /translation="MKWNYRLAIALLSCAAGAAQAAYRVEIDAPATLHRLLKKHLDLV
                     RYQDRADLSEEQLKFLCDTVSEQVAQLASTEGYLTPKTTVSMEQAPDRQEQPARRIRL
                     QVDPGPQTRITAVDISITGAAAQDDARRIAGIRRNWSLPIGAPFRQEDWDQAKNQGLE
                     KLQQKKYAAARIAQSQASIAPDDQQANLRVQYDSGPRFTLGRLQITGAKRYPESIIRN
                     VNPLNVGEEYDVNRLLSLQRQIQNTPYFSNVIVGIDNDPGHADMVPVKVEVTEFPTQR
                     IRTGFGYATDTGAQVEGRYTHYHVFERAYVFDSRFNLQQKRQHGQLNLAMPPDSRRFV
                     NSIDTSVEHSLLEGIDLRSLRVGVTRARNDELYDAAYTLIYYRDGLQQEDGAAPTGTV
                     IAPGKHQALVPGFSWARRDVDNPLFPRHGNLLTLETGFAVQGLLTNQSFGRLYGRFKQ
                     YLPASRRSLIILRTELGGVLTRGTASEVPASLLFRAGGNESVRGYSYNSIGNVQNGSV
                     YPTRYLLTGSAEYQHWLTATWGAALFYDVGAATDSWSSKTFYSGAGLGARWRSPVGPV
                     NLDLAYGIQNQRFQPHISLGIAF"
     gene            2628..>3241
                     /locus_tag="VFA14_03085"
     CDS             2628..>3241
                     /locus_tag="VFA14_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013234199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HZS80399.1"
                     /translation="MKTPVRLGTPLSPSAIKVMLLGSGELGKEVIIALQRLGVEVIAV
                     DRYADAPGHQVAHRAYVIDMTDGAALAELIAREQPDLVVPEIEAIATGTLVELEQAGK
                     ARVMPTARAAWLTMDREGIRRLASETLGLPTSPYRFADSLEELKAAAEQIGFPFVIKP
                     VMSSSGKGQSRVDSAAEIEAAWNHAVTGGRVDAGRVIVEGFIDF"
BASE COUNT          582 a          932 c         1030 g          697 t
ORIGIN      
        1 cgccttgtcg cagtgtcagt ttttgcagcg cgatgtcgcg caggttcagc gacaggggga
       61 aacgtagcga cgtgggcatg cgcgccggcg tcggcgcagt tgcctgtttg tcgatggtga
      121 ccgtgccgac gcgcaaatag gcgacgctca ggcagcgctg tatcagcgac agactccagc
      181 gggcgtcgat gcggtcgata tccagctgca tggcggcatt gcgataacgc aggtcgcgca
      241 ggctgaggcc cccggcaaga ttgccaccgg cataggttcc cgatagtttt ccctgcatca
      301 gccatacggc ggcgcgccag acactgcgtg taccgctgtc ggtgctagcc gcccagacca
      361 gtgccatgaa cagaatcagc agcagtgcca ggaggccgag caacagcccg ctgcgcctgt
      421 tgtttcgggg catgggcggc gtgggtgtat cggcgtcgat cgaggtcggt gcggtcatgc
      481 gtgtgtcgtc aaaaagcgat gccaagagaa atatgcggct ggaaccgttg gttttgtatg
      541 ccataggcca ggtccagatt gaccggaccg actgggctgc gccagcgcgc gccaaggcca
      601 gcgccgctgt agaaggtttt gctgctccag ctatcggtgg cggcgccgac atcatagaac
      661 agggcggcgc cccatgttgc cgtcagccag tgctggtact ctgcgctgcc ggtgagcaga
      721 tatctggtcg ggtaaacgct gccgttttgc acattgccaa tgctgttata gctgtagccg
      781 cgcaccgatt cgttgccacc ggcgcgaaac agcagagatg ccggcacttc cgacgctgta
      841 cccctggtca gcacgccgcc caactcggtg cgtagaatga tcaggctgcg cctggaggcg
      901 ggcagatatt gcttgaaacg tccatacagg cgtccgaagc tctggttggt gagcagacct
      961 tggacggcaa acccggtttc cagcgtcagc aggttgccat gacgcgggaa gaggggatta
     1021 tcgacatcgc gcctggccca ggaaaatcct ggcaccagcg cctgatgctt gcctggtgca
     1081 atgacggtgc cggtcggcgc ggcaccgtct tcctgctgca atccgtcacg ataatagatc
     1141 agcgtatagg ccgcgtcata cagctcgtca ttgcgtgcgc gcgtgacgcc gacgcgcagg
     1201 ctgcgcagat cgatgccttc cagcaggctg tgctccacgg aggtgtcgat gctgttgacg
     1261 aagcgcctgc tgtcgggcgg catagccaga ttgagctggc cgtgctgacg cttttgttgc
     1321 agattgaagc ggctatcgaa gacataggcc cgctcgaaga cgtggtaatg cgtatagcgt
     1381 ccttcgacct gggcgccggt atcggtggca tagccgaagc cggtacggat gcgctgggtc
     1441 ggaaattcgg tgacctcgac cttgaccggc accatgtcgg catgcccggg atcgttatcg
     1501 atgccgacga tgacattgct gaagtagggc gtgttctgga tttgccgctg cagcgacagc
     1561 aggcggttga cgtcgtattc ctcgccgaca ttgagcggat tgacattacg gatgatgctt
     1621 tccggatagc gtttggcgcc ggtgatctgc aatcgaccca aggtgaagcg tgggccgctg
     1681 tcatactgca cgcgcagatt ggcctgctga tcgtccgggg cgatgctggc ctgcgactgg
     1741 gcgatgcggg cagcggcgta ttttttctgt tgcagttttt ccagaccttg gttcttggcc
     1801 tggtcccagt cttcctggcg aaacggcgcg ccgatcggca aagaccagtt cctgcggatg
     1861 ccggcaatgc gtcgggcatc gtcctgcgcg gcggcgccgg tgatgctgat atcgactgct
     1921 gtgatccggg tttgcggtcc ggggtctacc tgcaggcgta tgcgccgcgc cggttgttcc
     1981 tgccgatccg gcgcttgttc catgctgacc gtggttttgg gcgtcaggta gccttccgtg
     2041 gatgccagtt gcgcgacctg ctcgctgacg gtatcgcaca gaaacttcag ttgttcctcg
     2101 ctcaggtccg cccgatcctg gtagcgcact aggtcgagat gttttttcag cagtcgatgc
     2161 agcgtagccg gggcatcgat ctcaacccga taagcggcct gcgccgctcc ggcggcgcac
     2221 gataacaggg caatggctaa tcgatagttc catttcattg gggtgtctgg catatgcgtt
     2281 tcttacggcg actacttgac aaaacaagct aatcgacctg gctgggtcaa ttcttcaggc
     2341 acgatcacct tggcgcccca gccttcgggc ggctgggtca ggctgacggg gctgtcgctc
     2401 atgctttctt ttccttgatt gtttctaata ccggacagtc ggttttctca cgctgcctcg
     2461 gaaaaaagac cgacgcggcc caatacatga ttgtgtgccg gatcaatagg ttgtaagaag
     2521 gccggtgtct gggcgtatat gatcgattcc atcaaagtct ggctgtccaa aacagccagg
     2581 atttggtatc gtcgcgcggt tcctcatttt atctcgtttg cattgtcatg aagactcctg
     2641 tccgcctcgg caccccgctt tccccttccg ccatcaaagt catgctgcta ggctctggtg
     2701 agcttggcaa agaagtcatc attgccctgc aacgcttggg ggtcgaggtg attgccgtgg
     2761 accgttacgc tgatgcaccg ggccaccagg ttgcgcatcg cgcctatgtg atcgacatga
     2821 ccgacggcgc tgcgctggcc gagctgattg ccagggagca acccgatctg gtggtgccgg
     2881 aaatcgaagc gattgccacc ggaacgctgg tcgaactgga acaagccggc aaagcccgcg
     2941 tcatgccgac ggcccgcgcc gcttggctga ccatggatcg cgaaggcatc cgccgtctgg
     3001 ccagtgagac gctgggcctg ccgacttcgc cttaccgctt tgccgacagc ctggaagaat
     3061 tgaaagctgc cgccgaacag atcggcttcc ccttcgtgat caagccagtc atgtcgtcgt
     3121 cgggcaaggg acagtccagg gtcgacagcg ctgccgaaat cgaagccgcc tggaaccacg
     3181 ccgttaccgg cggtcgtgtc gatgccggcc gggtgatcgt cgaaggcttc atcgatttcg
     3241 a
//
LOCUS       DASRDH010000136         2359 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1155423,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000136 DASRDH010000000
VERSION     DASRDH010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1155423"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..530
                     /gene="sucA"
                     /locus_tag="VFA14_03090"
     CDS             <1..530
                     /gene="sucA"
                     /locus_tag="VFA14_03090"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463288.1"
                     /note="SucA; E1 component of the oxoglutarate
                     dehydrogenase complex which catalyzes the formation of
                     succinyl-CoA from 2-oxoglutarate; SucA catalyzes the
                     reaction of 2-oxoglutarate with dihydrolipoamide
                     succinyltransferase-lipoate to form dihydrolipoamide
                     succinyltransferase-succinyldihydrolipoate and carbon
                     dioxide; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HZS80400.1"
                     /translation="RMFRKPLIIMTPKSLLRNKSASSPLSDLARGSFQTVIPEQDDKI
                     DAKKVKRIIACSGKVYYDLLNARKERAQFDTAIIRVEQLYPFPHKAFAAVLKQFPNFQ
                     ELVWTQDEPQNQGAWLQIQCNIFENLTEGKKLAYAGRPASASPAVGYYDKHYAQQKAL
                     IDSALSKLKGFVLTK"
     gene            606..1844
                     /gene="odhB"
                     /locus_tag="VFA14_03095"
     CDS             606..1844
                     /gene="odhB"
                     /locus_tag="VFA14_03095"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008329921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HZS80401.1"
                     /translation="MAHIEVKVPQLSESVAEATLLQWHKKVGEAVARDENLIDIETDK
                     VVLELPAPDAGVIIQIIKADGSTVVAGEIIAILDTDALPQVSPLEVKAAPVPQHTNEA
                     TPVAAPKLTGKSSIAMPAAAKILADNNMSIAQVTGTGKDGRVTKGDALASLNTPKPAA
                     AAAGSTLQQLAAPGKPGLENRPEERVPMSRLRARVAERLLQSQATNAILTTFNEINMQ
                     PVLDLRARYKDKFEKEHGVKLGFMSFFVKAVIHALKKYPIINASVDGNDIIYHGYFDI
                     GVAVGSPRGLVVPVLRDADQMSIAGIEKKIGEFGQKAKESKLSIEELTGGTFTVSNGG
                     TFGSMLSTPIINPPQSAILGIHATKDRAVVENGQIVIRPMNYFALSYDHRIIDGREAV
                     LSLVAIKEALEDPTRLLLDL"
     gene            1866..>2359
                     /locus_tag="VFA14_03100"
     CDS             1866..>2359
                     /locus_tag="VFA14_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZS80402.1"
                     /translation="MSKNFDVLVIGGGPGGYIAAIRAAQLGFHTACIDEWKNEKGDPA
                     PGGTCTNVGCIPSKALLQSSEHYEHAGHVFADHGIEVKGLGLNLEKMLGRKNAIVKQN
                     NDGILYLFKKNKVTFFHGRGSFVKGDANGYEIKLAGAMEESITARQVIVATGSSARAL
                     PDVP"
BASE COUNT          603 a          694 c          620 g          442 t
ORIGIN      
        1 tgcgcatgtt ccgcaagccg ctgatcatca tgacgccgaa gtcgctgtta cgcaacaaga
       61 gcgccagttc gccgctgtcc gatctggcca ggggttcgtt ccagaccgtc attcctgagc
      121 aggatgacaa gatcgacgcc aaaaaagtta agcgcatcat cgcctgctcc ggcaaggtct
      181 actacgatct gctcaatgcc cgcaaggaac gcgctcagtt cgatacggcc atcatccgtg
      241 tggaacagct gtatccgttc ccgcacaagg catttgccgc tgtgctcaag caattcccga
      301 atttccaaga attggtctgg acacaggacg aaccgcaaaa ccagggagca tggctgcaaa
      361 tccagtgcaa tatttttgaa aatctgactg aaggtaaaaa acttgcttat gcaggacgac
      421 cagccagcgc atcgccagcg gtcggctact acgacaagca ctatgcgcaa caaaaagcgc
      481 tgatcgatag cgcgctttct aagctcaaag gctttgtcct gaccaagtaa gctgacctgt
      541 tcggcgcgca tgcaaaggca atgcgcgctg atgttgatag aacaaacccg aatcggagaa
      601 atgacatggc acacatcgaa gtaaaagtcc cgcaattgtc agaatccgtt gcagaagcaa
      661 cgctgctgca atggcataaa aaggtgggcg aagccgtcgc gcgcgacgag aacctgatcg
      721 atatcgaaac agataaggtt gtgctggagt tgccggcccc tgacgccggc gtgatcattc
      781 aaatcatcaa ggccgacggt tcgaccgtgg tcgcgggcga aatcatcgcc atcctagaca
      841 ccgatgcgct cccacaagtg agtccgctgg aagtcaaggc tgctcccgtg ccgcaacaca
      901 ccaatgaagc gacaccggtc gccgcgccta aactgaccgg caagtccagc atcgccatgc
      961 ctgccgccgc caagatcctg gctgacaaca atatgtcgat agcgcaagtg accggcaccg
     1021 gcaaggatgg ccgcgtgacc aagggcgacg cgctggcctc cctgaataca ccaaagcctg
     1081 ctgctgccgc tgccggatcg acgctgcaac aacttgccgc tcctggcaag ccgggcctgg
     1141 aaaaccgtcc ggaagaacgc gtgccgatga gccgtctgcg cgcacgcgtg gcagagcgtc
     1201 tgctgcaatc acaagcaacc aacgccatcc tgaccacctt caacgaaatc aatatgcagc
     1261 ctgtgctgga cctgcgcgcc aggtacaagg acaagttcga gaaagaacac ggcgtcaagc
     1321 tgggcttcat gtccttcttc gtcaaggccg tgattcacgc actgaagaaa tatccgatca
     1381 tcaatgcgtc ggttgacggc aatgacatca tttaccatgg ctacttcgac atcggcgtcg
     1441 ccgtcggttc gccacgcggt ctggtggtgc cggttctgcg cgatgctgat caaatgtcga
     1501 tcgccggaat tgaaaaaaag atcggcgaat tcggacaaaa agccaaggaa agcaagctat
     1561 ccatagaaga actgaccggc ggtaccttca ccgtgtccaa cggcggcacc ttcggctcga
     1621 tgttatcgac accaatcatc aacccgccgc aatccgcgat tctgggcatc cacgccacca
     1681 aggaccgtgc cgtggtggaa aacggccaga tcgtgatccg tccgatgaat tacttcgcac
     1741 tgtcctacga tcaccgcatc atcgatggcc gcgaagccgt tctgagcctg gtcgccatca
     1801 aggaagcact ggaagaccca acccgcctgc tgctggatct ataatcgaaa cactgggata
     1861 aaaccatgag caagaatttt gacgtgcttg tcatcggcgg cggtccagga ggctacattg
     1921 ccgccatccg cgcagcgcaa ctgggttttc acaccgcctg tatcgacgaa tggaaaaacg
     1981 aaaaaggtga tccggcgcct ggcggcacct gtaccaacgt cggctgcatt ccttccaaag
     2041 cgctgctgca atcgtcagag cactatgaac acgctggcca tgtcttcgcc gatcacggta
     2101 tcgaagtcaa gggcttgggc ctgaatctgg aaaagatgct gggtcgcaag aacgccatcg
     2161 tcaaacaaaa caacgacggc attctgtacc tgttcaagaa aaacaaagtc acttttttcc
     2221 acggccgcgg cagtttcgtc aaaggcgatg ccaacggcta tgaaatcaag cttgccggcg
     2281 caatggaaga gtcgatcaca gccaggcaag tcatcgtcgc taccggttcc agcgcacgcg
     2341 cattacctga cgttccgtt
//
LOCUS       DASRDH010000137         2901 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_715626,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000137 DASRDH010000000
VERSION     DASRDH010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_715626"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="VFA14_03105"
     CDS             complement(<1..623)
                     /locus_tag="VFA14_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HZS80403.1"
                     /translation="MADGKIVQCIGAVVDVEFPRDAMPKIYDALKMEGSELTLEVQQQ
                     LGDGIVRTIALGTSDGLRRGMSIRNTGKGISVPVGKATLGRIMDVLGNPIDECGPVSH
                     AQTATIHRKAPAYDELSPSQELLETGIKVIDLVCPFAKGGKVGLFGGAGVGKTVNMME
                     LINNIAKAHSGLSVFAGVGERTREGNDFYHEMADAKVVDLDNPENSKV"
     gene            complement(681..1550)
                     /gene="atpG"
                     /locus_tag="VFA14_03110"
     CDS             complement(681..1550)
                     /gene="atpG"
                     /locus_tag="VFA14_03110"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HZS80404.1"
                     /translation="MATGKEIRGKIKSVENTKKITKAMEMVAASKMRKAQDRMYAARP
                     YSDKIHHIASNLSRANPEYTHPFMVKQDSARKVGFIIVTTDKGLCGGMNTNSLRLTMT
                     KIRELEAQGNAVEAVAIGNKGFSFLNRIGAKVVSCVTQLGDTPHLEKLIGPIKVLLDA
                     YQEGKLDAVYLSYTRFINTMRQEPVQQQLLPLASDSLAANQSAHSWDYLYEPDAQSVI
                     DELLVRYIEALIYQAVAENMASEQSARMVAMKAASDNAGNVIGELKLVYNKTRQAAIT
                     KELSEIVAGAAAV"
     gene            complement(1581..>2901)
                     /gene="atpA"
                     /locus_tag="VFA14_03115"
     CDS             complement(1581..>2901)
                     /gene="atpA"
                     /locus_tag="VFA14_03115"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005664034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HZS80405.1"
                     /translation="VLGGYEHISEGDTVKCTGRILEVPVGPELLGRVVNALGQPIDGK
                     GPVNAKLTAPIEKIAPGVIARQSVSEPMQTGLKSIDAMVPIGRGQRELIIGDRQTGKT
                     AVAIDAIINQKGQNVTCIYVAIGQKASSVKNIVRSLEAHGAMDYTIVVVASASESAAM
                     QYVSAYSGCAMGEYFRDRGEDALIVYDDLSKQAVAYRQVSLLLRRPPGREAYPGDVFY
                     LHSRLLERAARVNPDYVEKFTNGEIKGKSGSLTALPIIETQAGDVSAFVPTNVISITD
                     GQIFLETSLFNAGIRPAINAGISVSRVGGAAQTKVIKNLSGGIRTDLAQYRELAAFAQ
                     FASDLDEATRKQLDRGARVTELLKQAQYSPLPISLMAVTLFAVNKGYLDDVEVKKVLA
                     FEAGLHDFVKTSHMVLLQKIEDSRQLDKDTEAALAAAIAGFKKSGAY"
BASE COUNT          556 a          841 c          801 g          703 t
ORIGIN      
        1 accttggagt tttccgggtt gtccagatcg actaccttgg catccgccat ttcgtggtag
       61 aagtcgttac cttcgcgggt acgttcacca acaccggcaa acacagacaa gccgctgtgc
      121 gccttggcga tgttattgat cagctccatc atgttcaccg tcttgcccac ccccgcgccg
      181 ccgaacaggc cgactttacc gcccttggcg aacggacaga ccaggtcgat caccttgatg
      241 cctgtttcca gcagttcctg cgatggcgac agctcgtcgt atgccggcgc cttgcggtga
      301 atcgttgcgg tttgcgcgtg actcaccggg ccgcattcgt cgatcgggtt gcccagcaca
      361 tccatgatac ggcccagtgt ggccttgccg accggcacgc tgataccctt gccggtattc
      421 ctgatgctca tgccgcgacg cagaccatcc gaagtgccca gcgcaatggt gcgcacgata
      481 ccgtcaccga gctgttgctg cacttccagc gtcagttccg agccttccat cttcaaggcg
      541 tcgtaaatct tgggcatcgc gtcacgtgga aattcaacgt ccacgacagc gccgatacac
      601 tgaacgattt tgccatcagc cattttcgtt ccttcaataa tataaaatta aaacctgtca
      661 tgcatctctg catgacattt tcagaccgcg gctgcgccag cgacgatttc ggacaattcc
      721 ttggtgatcg ctgcctgacg ggttttgtta tagaccagct tcaattcacc aatgacattc
      781 ccggcgttat cgcttgctgc tttcatcgcg accatgcggg ccgattgctc ggatgccatg
      841 ttttcggcta cagcctggta gatcagcgct tcgatgtaac gcaccagcaa ctcgtcaatc
      901 acgctttggg catcgggttc gtagaggtag tcccatgagt gcgcgctttg atttgccgcc
      961 agactgtcgc tcgcgagcgg cagcagctgc tgctgcaccg gttcctgacg catcgtattg
     1021 atgaatctgg tatagctgag atagaccgcg tccagcttgc cttcctggta ggcatcgagc
     1081 agcaccttga tgggaccgat cagtttttcc agatgcggcg tatcgcccag ttgcgtgacg
     1141 cacgagacta ccttggcgcc gatgcgattc aggaagctga aacccttgtt gccgatggcg
     1201 acggcttcga ctgcattgcc ttgcgcttcc agttcccgaa tctttgtcat agtcagacgc
     1261 agcgagttgg tgttcatgcc gccgcacaga cccttgtcgg tcgtgacgat gatgaacccc
     1321 accttcctgg cactgtcctg tttgaccatg aacggatgcg tgtactctgg attggcacgc
     1381 gacagattgg aggcgatatg atgaatcttg tcactgtagg ggcgtgccgc atacatccgg
     1441 tcctgtgcct tgcgcatttt ggaggcagcg accatttcca tcgctttcgt gatctttttc
     1501 gtattttcca cgctcttgat cttgccgcgt atctctttac ctgtcgccat gagatggatt
     1561 ccttatcgct tcagtcgaga tcaatacgcg ccggattttt tgaaaccggc aatcgcagca
     1621 gccaatgccg cttcagtatc tttatcgagt tgtctgctgt cttcgatctt ttgcagcagc
     1681 accatgtggc tggtcttcac gaaatcgtgc agaccggctt cgaatgccag cactttcttg
     1741 acctcgacat cgtccagata acccttgttg acggcaaaca gcgtcacggc catcagcgaa
     1801 atcggcagtg gcgagtattg cgcctgtttc agcagttcgg ttacgcgtgc gccgcggtcc
     1861 agctgcttgc gggttgcttc gtccagatcg gaggcaaact gggcgaacgc tgccagttcg
     1921 cgatactgcg ccaggtcggt gcgaatgccg ccagacaggt ttttgatgac cttggtttgc
     1981 gcggcgccac cgacgcgcga caccgaaata ccggcattga tcgcaggacg gataccggcg
     2041 ttgaacagcg atgtttccag gaagatttga ccgtcggtga tcgaaatcac gttggtggga
     2101 acgaatgcgg acacatcgcc ggcctgtgtt tcgatgatgg gcagtgctgt cagagaaccg
     2161 cttttgccct tgatttcgcc attggtgaat ttctcgacgt agtcgggatt gacgcgcgcc
     2221 gcgcgttcca gcagacggct gtgcaggtag aacacgtcgc ctggataggc ttcgcggccc
     2281 ggcggacggc gcagcagcag ggaaacctga cggtaagcaa cggcctgctt ggacagatcg
     2341 tcatagacga tcaatgcatc ttcaccgcga tcgcggaagt attcgcccat ggcgcagcca
     2401 gagtaggccg acacgtattg catggccgcc gattcggaag ccgacgcgac gacgacgatg
     2461 gtgtaatcca tcgcgccgtg cgcttccagc gagcgcacga tgttcttgac cgatgatgct
     2521 ttttgaccga tcgcgacata gatgcatgtc acattctggc ccttctggtt gatgatcgca
     2581 tcgatcgcga cggcagtctt gccggtttga cgatcgccga tgatcagctc gcgttgacca
     2641 cgaccgatag gcaccatcgc atcgatcgac ttcaggccgg tttgcatcgg ctcggagacg
     2701 gattgacgcg cgatgacccc gggagcaatt ttttcgatcg gggctgtcag tttggcattg
     2761 acggggcctt tgccgtcgat cggctgtccc agcgcattga ccacacggcc aagcagttca
     2821 gggccgaccg gtacttccag gatacggcct gtgcatttga ccgtatcgcc ttcggaaata
     2881 tgttcgtatc cgcccagaac g
//
LOCUS       DASRDH010000138         2745 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_655004,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000138 DASRDH010000000
VERSION     DASRDH010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_655004"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="VFA14_03120"
     CDS             <1..277
                     /locus_tag="VFA14_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007879170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HZS80406.1"
                     /translation="QRNKRIKQLEERSRQEQQTQLLIETPYRNGVMLEALANACNSAT
                     LISVATDLTLATEVVRTQTARQWRSDIAAGKTPDFHRKPSVFLLLAD"
     gene            complement(285..1304)
                     /locus_tag="VFA14_03125"
     CDS             complement(285..1304)
                     /locus_tag="VFA14_03125"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HZS80407.1"
                     /translation="MSNNQSDNPEVPASGPAEPVFPPATAAGKGGWERDVLEKLLLFT
                     VKEQRAHRRWSIFFKVAGLVVFVFAIWSVFNYNRSDIELVGRHTALVEISGVIDAEGR
                     SSASAVMAALGKAFSATGSVGVILKINSPGGSPVQAGMINDEIMRLRKAYPKKPLYVV
                     VEEVCASGGYYIAAAADRIYVNKASIVGSIGVLMDGFGFTGLMDKLGVERRLLTAGEN
                     KGFLDPFSPQDTRQKKFAQQMLGEVHQQFIDVVRKGRGQRLKETPEIFSGMFWIGSKA
                     VTMGLADGFGTVDSVARDVIEAEDLVDYTQTEGLPERVLKKFGVAVGSGVVKSMLDGS
                     GIALR"
     gene            complement(1481..2140)
                     /locus_tag="VFA14_03130"
     CDS             complement(1481..2140)
                     /locus_tag="VFA14_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008327891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HZS80408.1"
                     /translation="MARKRFDLIVFDWDGTLMDSTSTIVECIQAAARDLGLPVPDRSA
                     ASHVIGLGLQDAIQAAIPDVDPKYYSRIIERYRHHYFGQDRALKLFDGVREMLDDLGR
                     QTYFLAVATGKSRIGLDRALDVTGLVSAFDATRCADETFSKPHPAMLQELTRALGQDM
                     ERTLMIGDTTHDLQMAMNAGAAGVAVEFGAHPAQQLQALHPLYSAGSIQALHRWLDEN
                     A"
     gene            complement(2144..>2745)
                     /locus_tag="VFA14_03135"
     CDS             complement(2144..>2745)
                     /locus_tag="VFA14_03135"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007879177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HZS80409.1"
                     /translation="FLELVHRLDRDTSGILLLAKKRSALTSLHEQIRDGLTDKRYLTL
                     VRGSWKNTRQHIRLPLRKYSTADGERRVRVQEDGQASHTVFSLVRRYGEYALLEAELK
                     TGRTHQIRVHLSASGFVIVGDAKYGDFVLNKALQKADGERVAFRRMFLHAHRISFRHP
                     GSGAPVTLEAGLPPECLRFLNSLDAAASGAGLFIESLEK"
BASE COUNT          610 a          856 c          732 g          547 t
ORIGIN      
        1 gcaacgcaac aagcgcatca aacagctgga agaacgttca cggcaagagc aacagacgca
       61 attgctgatc gagacgccct accgcaacgg cgtcatgctg gaagccctgg caaacgcctg
      121 caacagcgcc acattgatca gcgtggccac cgatctgaca ctggcgaccg aggtcgtcag
      181 gacgcagacg gcgcgtcaat ggaggagcga cattgctgcc ggaaaaacgc cagacttcca
      241 caggaagccc agcgtctttc tgctgctggc cgattgacag gttttcagcg cagcgcgatg
      301 ccgctgccat ccagcatgga tttgacaacg ccgctgccga ccgccacgcc gaatttcttc
      361 aacacgcgtt ccggcaagcc ttcagtctgg gtatagtcca ccagatcttc ggcctcgatg
      421 acgtcgcgcg ctacgctgtc gaccgtgcca aagccatcgg ccagtcccat tgtgaccgct
      481 ttggaaccga tccagaacat gcccgagaaa atttccggcg tttccttcag acgctggcca
      541 cgacctttgc gcaccacgtc aatgaattgc tggtgaacct ccccgagcat ttgctgcgcg
      601 aatttttttt gccgggtgtc ttgcgggctg aacggatcaa gaaaaccttt gttctcgccg
      661 gcggtcagca gtcgacgttc gacaccaagc ttgtccatca ggccggtgaa gccaaaacca
      721 tccatgagca caccgatgga accgacgata ctggctttat tgacgtagat cctgtcggct
      781 gccgctgcaa tataataacc gccggaggcg cacacttcct cgaccacgac gtatagtggc
      841 ttcttcggat aagccttgcg cagacgcatg atttcatcat tgatcatgcc agcctgcacc
      901 gggctgccgc cggggctgtt gatcttgagg atcactccca cggaaccagt agcggaaaac
      961 gctttgccta gcgccgccat caccgccgat gccgagctgc ggccttcggc gtcgatgaca
     1021 ccgctgattt cgaccagcgc ggtgtgcctg ccgaccagct caatgtcaga cctgttgtaa
     1081 ttgaagacgc tccagatcgc aaaaacaaaa acgaccaagc cagcgacctt gaagaaaata
     1141 ctccagcgac ggtgcgcgcg ctgctctttg accgtaaaca gcagcagttt ttccagcacg
     1201 tcgcgctccc agccgccctt ccctgcggcg gtggctggcg gaaagaccgg ttcagccggt
     1261 ccggatgctg gcacttcagg attgtcagat tggttattac tcatatcgtc acttgcaacg
     1321 gcagcccgta ttcgaggtcg tcgacgcagc tgcctcggtt tccacatggc ttagagccgc
     1381 taacacaacg cagccaacgg cgtcatcagc gactttcatg ccggcgcgag ctttatatta
     1441 tcgtggaggt tcaggaaata ccccagtctt tttcaaacac ctaggcattt tcatccagcc
     1501 atcgatgcag cgcctggatc gaaccagccg aatacagagg atgcagggcc tgcaactgct
     1561 gcgccgggtg cgcgccgaat tctaccgcaa ccccggcggc gccggcattc atggccatct
     1621 gcaaatcgtg ggtggtgtcg ccaatcatca gcgtgcgctc catgtcctga ccaagcgcgc
     1681 gggtcagttc ctgcagcatg gccggatgcg gcttggagaa agtttcatcg gcgcagcgcg
     1741 tagcgtcgaa cgctgacacc agcccagtca cgtccagcgc gcgatcaagg ccaatgcgac
     1801 tcttgccggt ggcgaccgcc agaaaataag tttgtcggcc gagatcgtcc agcatctccc
     1861 tgacaccatc gaacagcttc agcgcccgat cctgaccgaa ataatgatgg cgatagcgct
     1921 cgatgatgcg cgagtaatac ttcggatcga catcgggaat cgctgcctgt atggcgtcct
     1981 gcaaacccag accgatcaca tgggaagccg cgctcctgtc cgggaccggc aggcccagat
     2041 ccctggccgc agcctgtatg cattcgacga tggtcgatgt gctgtccatc aacgtgccgt
     2101 cccagtcgaa cacgatcaaa tcaaaccgtt ttcttgccat gtttcacttt tcaagagact
     2161 cgataaaaag acccgccccg gaggcagcag catccaggct gttcaggaag cgcaagcatt
     2221 ccggcggcaa ccctgcctcc agcgtcaccg gcgcaccact gccgggatgc ctgaaactga
     2281 tccggtgggc atgtaaaaac atacgcctga acgcgacccg ctcaccatct gctttctgca
     2341 aggctttatt caggacgaaa tcgccatatt tggcgtcgcc gacgatgaca aaaccgctag
     2401 ccgacaaatg cacgcgtatc tgatgcgtgc gcccggtttt cagttccgcc tccagcaaag
     2461 catattcgcc atagcggcgg accagcgaaa acacggtatg cgaggcttga ccgtcttcct
     2521 gcacgcggac gcgccgctca ccatcggcag tactatattt acgcaatggt aatctgatat
     2581 gctggcgcgt gtttttccag ctaccacgga ccagcgtcag ataacgcttg tcggtcaggc
     2641 cgtcgcggat ctgttcatgc aggctggtca acgccgagcg cttcttcgca agcagcaaaa
     2701 ttcccgaggt atcgcggtcg aggcgatgca ccagttccag aaatc
//
LOCUS       DASRDH010000139         2113 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_308668,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000139 DASRDH010000000
VERSION     DASRDH010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308668"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1714)
                     /gene="uvrA"
                     /locus_tag="VFA14_03140"
     CDS             complement(<1..1714)
                     /gene="uvrA"
                     /locus_tag="VFA14_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008332131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HZS80410.1"
                     /translation="MEEIRIRGARTHNLKNISLDLPRNKLIVITGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSSYARQFLQLMEKPDVDMIEGLSPAISIEQKATSHNPRSTVGTVT
                     EIHDYLRLLYARAGMPFCPNHPENPLAAQSVSQMVDAVLAMPEDTRLMILAPVVANRK
                     GEHADLFGQMQAHGFVRFRIQSGAGAARVHEIDDLPKLRKTEKHTIDVVIDRIKVRDG
                     IKQRLAESFETALRIAEGRAIALEMDTGKEHLYSSRFACPVCGYSLQELEPRLFSFNN
                     PMGACPECDGLGHIDFFDPQRIVAFPDLSLASGAIKGWDRRNQFYFQMLSNLAAFYEF
                     DIDMPFEQLPEKARQVILYGSGRQTLPFTYANERGRAVIKEHSFEGVVCNLQRRYRET
                     DSMAVKEELARFINKKACPSCQGTRLRVEARFVKVGAGSQQRAIYEVAAMPLRETLSF
                     FENLELTGTRKEIADRIIKEIVSRLRFLNNVGLDYLSLERSADTLSGGEAQRIRLASQ
                     IGSGLTGVMYVLDEPSIGLHQRDNDRLIGTLRHLRDIGNSVLVVEHDEDAIRTADYVV
                     DMGLG"
BASE COUNT          454 a          598 c          593 g          468 t
ORIGIN      
        1 cgcccaaccc catatcgacc acatagtcgg cagtgcgaat ggcgtcttcg tcatgttcca
       61 ccaccagcac gctattgccg atgtcgcgca gatgcctgag cgtgccaatc agccggtcat
      121 tgtcgcgctg gtgcaggccg atcgacggtt catcgagtac gtacatcacg ccagtcagac
      181 cggaaccgat ttgcgaagcc agacgaatgc gctgcgcttc gccaccggac agcgtatcag
      241 cgctgcgttc gagcgaaaga taatcgaggc cgacattgtt gaggaatctc aggcgtgaga
      301 cgatttcctt gatgatacgg tcagcgattt ctttcctggt gccggtcagc tccagattct
      361 cgaagaagga caaggtctcg cgcagcggca tggccgccac ctcatagata gcgcgctgct
      421 ggctgcccgc gccgaccttg acgaagcgtg cttccacacg cagacgcgta ccctggcagg
      481 aagggcatgc ctttttgttg atgaatctgg ccagttcttc cttgaccgcc atcgaatcgg
      541 tttcacgata gcggcgctgc aggttgcaga ccacgccctc gaagctatgc tccttgatca
      601 cggcacgacc gcgttcgttc gcataggtga agggaagcgt ctggcggcct gaaccgtaca
      661 agattacttg ccgggctttt tccggcaatt gctcgaacgg catgtcgata tcgaattcat
      721 agaatgccgc cagattcgac agcatctgga agtaaaactg attgcggcgg tcccagccct
      781 tgatggcgcc gctggccagc gacagatcag ggaaggcgac gatgcgttgc ggatcaaaaa
      841 aatcgatgtg gcccaagcca tcacattccg ggcaggcgcc cataggattg ttgaacgaga
      901 acaggcgcgg ctccagttcc tgcaacgaat aaccacacac cggacaagcg aatctgctgg
      961 aatacaagtg ttccttaccg gtatccatct ccagtgcgat ggcgcgacct tcggcgatac
     1021 gcagggcagt ctcgaaactt tccgccagac gctgcttgat gccatccctg accttgatgc
     1081 ggtcgattac cacgtcgatg gtgtgctttt cggttttcct gagtttgggc aagtcatcga
     1141 tctcatggac cctggcggcg ccagcgccgc tttgaatgcg aaagcgcacg aagccgtgcg
     1201 cctgcatctg tccaaacaga tcggcatgct cgcccttgcg gttggccacg accggcgcca
     1261 ggatcatcag tctggtgtct tccggcatgg ccagtacggc atcgaccatc tgcgacaccg
     1321 attgcgccgc cagcggattt tccggatgat tggggcaaaa gggcataccg gcgcgggcgt
     1381 acagcagacg caggtaatca tggatttcag tgacagtgcc gacggtagag cgcgggttgt
     1441 gcgaagtcgc tttctgctcg atcgagatcg ccggagacag gccttcgatc atatcgacgt
     1501 cgggtttttc catcaactgc aaaaactggc gcgcatagga agaaagcgat tccacgtaac
     1561 gtcgctggcc ttccgcgtac agcgtatcga acgccagcga cgattttcct gaaccggaca
     1621 ggccagtgat cacgatcagt ttattacgcg gtaaatcgag gctaatgttc ttcagattgt
     1681 gcgtacgtgc gccacgaatg cgaatctctt ccatgaatgc cttcagcgtg agttttccat
     1741 gtgacacccc ttgcctgtat gcattgcatt gaacggaggg ccagcctgct actatagcag
     1801 gacttggcat ttgcggctga ccgataagat accggcaaac atgaacaagg cgagtgggcg
     1861 attatttggc agacactgct cgaaaaatgg ccggcagttt gatagctgat atcatggcgg
     1921 gtttagacat ccgtcacaat gcagccggat tgtctgcttg cccagctgcc actcattcat
     1981 gcacaccaac gcttccgaat ccgtacagaa agctggcatg tccggcgctg aaatccgtgc
     2041 cagcgtgtcg ctcgcatcgg ttttttcctt gcgcatgctg ggattgttcc tgattctgcc
     2101 ggtgtttgcg ata
//
LOCUS       DASRDH010000140        12655 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1384659,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000140 DASRDH010000000
VERSION     DASRDH010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 12655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12655
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1384659"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            34..558
                     /locus_tag="VFA14_03145"
     CDS             34..558
                     /locus_tag="VFA14_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HZS80411.1"
                     /translation="MEHYTKPAILLHWLIALLIMVTFFLGLTMVDIPGLTPTKLKYFS
                     WHKWLGVTVLALACLRLLWRLTHAAPAYPIEMPRWQQSAAHVLHALLYILIFAIPISG
                     YFYSMAAGIPVVYLGLLPLPVFIDPNPMLKPLLQMTHYWLNMTLASAVCLHILAALKH
                     LLIDRDGIFKRILP"
     gene            580..1170
                     /locus_tag="VFA14_03150"
     CDS             580..1170
                     /locus_tag="VFA14_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HZS80412.1"
                     /translation="MISRSALIRIVASSFAVLGLACSAIAAPLRIDAGKSTVSVAFRQ
                     MNVPVEATFRKFTARIDFDVTRPADAKASIDIDIPSFDLGDAGYNRDVLKKEWFDVAQ
                     FPKASFVASTIRASAGAPAGTGYDVAGKLTIKGKTADVRFPLTVRQDKGGRTFDGAFA
                     IKRLAFNIGEGEWKDTGMVADEVTIRFHVVTTSSSQ"
     gene            1216..1788
                     /locus_tag="VFA14_03155"
     CDS             1216..1788
                     /locus_tag="VFA14_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013232720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HZS80413.1"
                     /translation="MKLIFTLISLLAGVASTSVLAADTYTIDPSHTYPSFEADHMGIS
                     IWRGKFTKTSGTVTLDRAARTGAVDITIDASSIDFGFPKLNEHAKSADMFDVQKFPTA
                     TYKSTSIQFDGDRPVMVNGELSLHGVTRTVPLHINKFACIQHPMLKREVCGADASATI
                     DRADFGISYGAPRFAMETKLNIQIEAIKAN"
     gene            1943..2578
                     /gene="pdxH"
                     /locus_tag="VFA14_03160"
     CDS             1943..2578
                     /gene="pdxH"
                     /locus_tag="VFA14_03160"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006461726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HZS80414.1"
                     /translation="MSIADLRKDYSRASLSELDVAPDPIAQFSMWLDEAIKAEIPEPT
                     AMSVATVGPDGKPSSRIVLLKQIDAAGLTWFTNYHSRKGSEIAQHPYVALLFHWVELE
                     RQVRIEGKAERLAASESDAYFYSRPLASQLSAIASAQSQPVDRRETLEAQLAVAQQKY
                     GAQPVRPEYWGGYRVVPEKLEFWQGRRSRLHDRVVYTRTTDGAWSRQRLQP"
     gene            complement(2538..3608)
                     /locus_tag="VFA14_03165"
     CDS             complement(2538..3608)
                     /locus_tag="VFA14_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019699878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZS80415.1"
                     /translation="MSTSVLTFFNNKGGVGKTSLIYHLAWMFALLHKRVVIIDLDPQA
                     NLTAAFLDENQIEEIWKQENTGSTIYQCIKPLMGVGDIAEPVLQNIATDLYLLPGDVN
                     LSSFEDELSAAWPGSMNDSNLYRPLRILTAFWQVTQMAASKVQADIVLVDIGPNLGAI
                     NRSVLIATDYVAIPLGADLFSLQGLKNLGPTLKSWRNLWNKRLGNWSDSAEKKSFPDF
                     RLPDGKMQAIGYLCQQHSIRLDRPVQAYDKWVQRIPAVYRESVLSDEVPNDVRQEDDP
                     YCLATIKHYRSLIPIGQEHRKPIFKLTTADGAIGSHFGAVQDAKKDFTQLANKIIEQM
                     EMSKKRNATIRVADAAGSRRHP"
     gene            complement(3605..4573)
                     /locus_tag="VFA14_03170"
     CDS             complement(3605..4573)
                     /locus_tag="VFA14_03170"
                     /inference="COORDINATES: protein motif:HMM:NF025124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZS80416.1"
                     /translation="MKTDGNILPLPVLSAEKRILKGSEITVVTVMPSDMPPLKYDGRI
                     WIRTGPRRSLANEQEERILNEKRRYKNLPFDIHPLPSARLSDLSRVIFENEYLPQAFA
                     PDVLEANGRSYEERLASCRMIVAPDDTTPTITGMLAISKRPQDFLPGAYVQFLRIDGI
                     ELSDPVIDEAEIGGGIVDILRRTEEKLKAHNRAAVDITSAATHQVDMPYPQIAIQQVL
                     YNAVLHRTYESTNAPIRVYWFNDRIEINSPGGPYGNVTAENFGKPGITDYRNPNIADV
                     LKTFGFIQAFGRGIAISRQAMGLNGNPAPEFNTSQSAVVCILRGKS"
     gene            complement(4595..4804)
                     /locus_tag="VFA14_03175"
     CDS             complement(4595..4804)
                     /locus_tag="VFA14_03175"
                     /inference="COORDINATES: protein motif:HMM:NF016232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZS80417.1"
                     /translation="MTDQELLDLLDDTESDRTERKETFKGDITKKARQTVCAFANDLP
                     GHNRPGVLFIGAKDDGKPSGLEITD"
     gene            5018..6931
                     /gene="thiC"
                     /locus_tag="VFA14_03180"
     CDS             5018..6931
                     /gene="thiC"
                     /locus_tag="VFA14_03180"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018058147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HZS80418.1"
                     /translation="MNVNPKFLSATATVDEAAVQPLPNSRKIYVTGSRPDIRVPMREI
                     SQSDTPAMFGTEKNPPIYVYDTSGPYTDPEVRIDIRAGLATPRLPWILERNDTEELAG
                     PTSEYGQQRLNDPGLAELRFKLRRKPRRARAGGNVTQMHYARRGIITPEMEFIAIREN
                     LRRQEYLEQLRASGPMGGKLADQMGRQHPGQSFGASLPAVITPEFVRDEVARGRAIIP
                     ANINHPEVEPMIIGRNFLVKINANIGNSAVTSSIGEEVEKMTWAIRWGGDNVMDLSTG
                     RNIHETREWIIRNSPVPIGTVPIYQALEKVKGKAEDLTWEIFRDTLIEQAEQGVDYFT
                     IHAGVRLQFVPLTARRMTGIVSRGGSIMAKWCLAHHRESFIYEHFEDICEIMKAYDVS
                     FSLGDGLRPGSIYDANDEAQLGELKTLGELTRIAWKYDVQVMIEGPGHVPMHLIKENM
                     DLQLEQCHEAPFYTLGPLTTDIAPGYDHITSGIGAAQIGWYGTAMLCYVTPKEHLGLP
                     NKTDVKDGIITYKIAAHAADLAKGHPGAQIRDNALSKARFEFRWEDQFNIGLDPDKAR
                     EFHDETLPRDAAKVAHFCSMCGPHFCSMKITQEVRDYAAREGIPETDALRQGMAVKSV
                     EFVEMGAEIYSRA"
     gene            6967..7167
                     /gene="thiS"
                     /locus_tag="VFA14_03185"
     CDS             6967..7167
                     /gene="thiS"
                     /locus_tag="VFA14_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HZS80419.1"
                     /translation="MQIELNGKPHVVAEGADLAQLIAALDLTGKAVAVAVNRQVVPSQ
                     SWAGRMLVETDRVDVVRAIGGG"
     gene            7175..7978
                     /locus_tag="VFA14_03190"
     CDS             7175..7978
                     /locus_tag="VFA14_03190"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HZS80420.1"
                     /translation="MSDLPALSTDAGLTIAGETYGSRLLVGSGKYTDLDEMRAATEAS
                     GADIITVAIRRVNIGQDPHAPSLLDALPPLRYTILPNTAGCYNAEDAVYTLQLARELL
                     HGHKLVKLEVLGDEKSLFPNMPETLKAAAMLVKDGFDVMVYCSDDPVQARMLEDLGVA
                     ALMPLASLIGSGMGILNPWNLSLIIEQSNIPVLVDAGVGTASDAAIAMELGCAGVLMN
                     TAIAGARDPVRMARAMKLAVAAGREAFLAGRIAKRFAASPSSPMAGRMT"
     gene            7975..8829
                     /locus_tag="VFA14_03195"
     CDS             7975..8829
                     /locus_tag="VFA14_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HZS80421.1"
                     /translation="MKQALRAYRTSPPCVLVFSGSDPSGGAGMQADIPAISALGCHPL
                     SVPTALTVQDNVRVFAVHALEAELIRHQAQVLIDRFQISAVKLGIAGNRRNAAMIAEL
                     ILQLRARQPGLPVVFDPVLANGKGDCLSSDDAALALEPLYAVATVITPNLDEAHRLCG
                     SEHAPEQQAALLMARGCGHVLLKGGHGPQHQDVINRWFGPDGEGASWRWPRLPDAFHG
                     SGCTLAAALSAQLAHGIDMRAAIEAAQRYCQQTLETSYAIAAGQRIPDRVPLFAGENG
                     PRGIILET"
     gene            8842..9618
                     /locus_tag="VFA14_03200"
     CDS             8842..9618
                     /locus_tag="VFA14_03200"
                     /inference="COORDINATES: protein motif:HMM:NF005063.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HZS80422.1"
                     /translation="MRLGFLGAGTISNAVIRGICRGDMAEALGIEVIFVSQRNAQIAA
                     KLAKDFACIKITEDNQQLVDNADLVFVALPGAVCEQALRALQFRADQKLISFVPNPSC
                     AELAEWTGGKLPVLRAVPLPFIAEHKTATPIFPADKDLQALFGATGGCILAEEEKQFN
                     FFMTAGSMMGVYYRFCGLCTSWLSQRGLPEKQSMLYMTRLFRALSEEAVKGKDFAALQ
                     QEYSTKGGTNELIAKLFEDKGGVEALLAALEEGFARINGK"
     gene            9656..10291
                     /gene="thiE"
                     /locus_tag="VFA14_03205"
     CDS             9656..10291
                     /gene="thiE"
                     /locus_tag="VFA14_03205"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HZS80423.1"
                     /translation="MNDASRQWHLRGLYIVTPDWDDTEKLVNVTAQAIEGGAALVQYR
                     HKTAAAALRAEQAGALLKICRRCNTPLIINDDIDLCLALDADGIHVGGADAPVAQARA
                     KVGRDRIVGASCYGALQLARDAHVAGASYVAFGGFYPSRVKQYGFRTAPGIVAESKKE
                     IPLPVVVIGGMTQENCLPLIRQGADMVAAISSIYMTEEAAREAACRFAGLF"
     gene            10329..12620
                     /locus_tag="VFA14_03210"
     CDS             10329..12620
                     /locus_tag="VFA14_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007882952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HZS80424.1"
                     /translation="MQNLLQHLNDGQLAAVTLPAQSALILAGAGSGKTRVLTTRIAWL
                     IQTGQVSPSGILAVTFTNKAAKEMLTRLSAMMPINPRGMWIGTFHGLCNRLLRAHHRD
                     AALPQTFQILDTQDQSSAIKRLFKTMNIDDEKYPPRDLMYFINGAKEQGLRAGDIDTH
                     DDYNRRFVELYDLYDQQCQREGVVDFAELLLRTYELLSRNQPLREHYQRRFRHVLVDE
                     FQDTNDLQYKWLKLISGQGRIDSSAVFAVGDDDQSIYAFRGANVGNMSAFEREFRVGN
                     LIRLEQNYRSHGHILDVANVLIANNSRRLGKNLRTDAGLGEQVRVFEADSDLQEAQWL
                     IEQVRDLIGQGWMRSEIAVLYRSNAQSRVLERALFSAAIAYRVYGGLRFFERAEIKHA
                     IAYLQLLDNPHNDSAFLRVVNFPARGIGARTLEQLQDAARQYDCSLYAAVPYVAGKAG
                     SALRAFIKLIEGVRCGTQHLPLPEMLQVVLDASDLMTHYQNEKDGDGRIENLKELISD
                     ATQFVSAEGYGLDTPAFSGPQAPAVAGASITTADGIDIIDADAMLTALMSPLSAFLSH
                     ASLEAGDNQAQAGQDALQLMTVHAAKGLEFDAVFITGLEEGLFPHDNSAKEDGGVEEE
                     RRLMYVAITRARKRLYMSFSQTRMLRGQTRYHRRSRFFDELPDASLKWLSPKIQQHSW
                     FAHPKAAWDDVSESGNNRIAQNFRRSDTGWRIGQNVTHARFGEGVIVNIEGTGVHVRA
                     HINFGKSGMKLLDLSVAKLEPVG"
BASE COUNT         2684 a         3395 c         3752 g         2824 t
ORIGIN      
        1 ccactgccac tgccattcgt ttgagcgacc tccatggaac actacaccaa acctgccatc
       61 ctgctgcatt ggctgatcgc gctgctgatc atggtcacgt tttttctcgg cctgaccatg
      121 gttgacattc ccggtctgac accgaccaag ctcaaatatt tttcctggca caagtggctt
      181 ggcgtcaccg ttctggcctt ggcatgtctg cgtctgctgt ggcgtctgac ccatgcggcg
      241 ccggcctatc cgatagagat gccgcgttgg cagcaaagcg cggcgcatgt actccatgcc
      301 ttgctctata ttctgatttt tgcgattcca atctcaggct atttctatag catggcagcg
      361 ggaatcccgg tagtgtatct gggtttgctg ccattgccgg ttttcatcga tcccaacccg
      421 atgctcaaac ctttgctgca gatgacgcac tactggctga acatgacgtt ggcaagcgct
      481 gtctgtctac acatattggc tgcgctgaag cacctgctca tcgatcgtga cggcatattc
      541 aaacgcatcc tgccctgatc tctttgccta ggacatatcg tgatttcacg ttccgcattg
      601 atccgtatcg tcgccagcag cttcgctgtc ttgggccttg cctgttccgc catcgccgcg
      661 ccgctcagga tcgatgccgg caaaagcacg gtcagcgtcg cattcaggca aatgaatgtg
      721 ccggttgaag ccacattcag gaagttcacg gcacggatcg atttcgacgt caccaggccc
      781 gccgacgcca aagccagtat cgacatcgac atccccagct tcgacctggg tgatgccgga
      841 tataacaggg acgtactgaa aaaagaatgg ttcgatgtcg cgcagtttcc caaagccagc
      901 tttgtcgcca gtacgatcag ggcgagcgct ggcgcgccgg ccgggaccgg atacgatgtc
      961 gccggcaagc tgaccatcaa gggcaagact gccgatgtac gctttccgct gacggtcagg
     1021 caagacaagg ggggccggac cttcgacggc gccttcgcca tcaaacggct ggcgttcaat
     1081 atcggtgaag gcgaatggaa agacaccggt atggtcgcgg acgaagtcac catcaggttt
     1141 cacgtcgtca ccacatcatc atcgcagtga tcatcatctt ttcaacgctc gacaaatcat
     1201 gcaaaggacc acttcatgaa attgatattc accttgatct ccctgcttgc cggcgtcgcg
     1261 tcgacctccg ttctggcagc cgacacctac accatcgacc ccagccacac ctatccaagt
     1321 tttgaagccg accacatggg catttccatc tggcgcggca aatttaccaa aaccagcggc
     1381 accgtgacgc tggaccgcgc cgccaggacc ggcgccgtgg acatcacgat cgatgcttcg
     1441 tcgatcgatt tcggctttcc aaaattgaac gaacatgcca aatccgcgga tatgttcgac
     1501 gtgcagaaat ttccgaccgc gacctacaag agtacttcca tacagttcga tggtgacagg
     1561 ccggtgatgg tcaatggcga actaagtttg cacggcgtca ccaggacggt tccattgcac
     1621 atcaacaaat ttgcttgcat tcagcacccg atgctcaagc gggaagtctg cggcgccgat
     1681 gccagcgcga cgatcgatcg cgctgacttc ggtatcagct atggcgcgcc acgttttgcg
     1741 atggaaacca agctgaacat tcagatcgag gcaatcaagg ctaactgatt tcacccccct
     1801 ccttcggggc tggtacactc gtgggtgtat cagctttttt gtttacgtcg agtttcactc
     1861 cagccttctg ccatataatg gttcgcgtca acctttaaat gcgctacgct gatgcagtcc
     1921 cccgatcaaa gataaccgcg acatgtcgat agcagatctt cgcaaggatt acagtcgcgc
     1981 cagcttgtct gaactcgatg tcgccccgga tcccattgcg caattttcaa tgtggctgga
     2041 tgaagcaatt aaagcggaga ttcctgaacc cactgccatg agcgtcgcta ccgtcggccc
     2101 cgacggtaaa ccatcgtcgc gcatcgttct gctcaagcaa atcgatgctg ccggtttgac
     2161 atggttcact aactatcaca gccgcaaagg gagcgaaatc gcgcagcatc cgtatgtcgc
     2221 gctgctgttt cactgggtag aactcgaacg gcaggtccgt atcgaaggca aggccgagcg
     2281 gcttgcagcg agcgagagcg atgcttattt ttacagccgt ccgctggcca gccagttgag
     2341 cgcgatcgct tcggcgcaaa gccagccggt cgacaggcgg gaaacgctgg aagcgcaact
     2401 cgccgttgcg caacagaaat acggcgcgca accggtccgt cctgaatact ggggcggcta
     2461 ccgtgtcgtg ccggagaaac tggagttctg gcaaggccgt cgctcacgac tgcatgatcg
     2521 cgttgtctat acacgcacta cggatggcgc ctggagccgg cagcgtctgc aaccctgatc
     2581 gtagcattgc gttttttaga catttccatc tgctcgatga ttttatttgc caactgtgta
     2641 aagtctttct ttgcatcttg cacggcgccg aaatggctgc cgatagcgcc gtcagccgtg
     2701 gtaagtttga aaataggctt gcggtgttct tggcctatgg gaatcagact gcggtagtgc
     2761 ttgattgtcg ccaggcagta aggatcatct tcctgtcgca catcgttggg tacttcatca
     2821 ctcaatactg attcccgata taccgccgga atgcgttgca cccatttgtc ataggcttgt
     2881 accggtctgt ccagacgtat gctgtgttgt tgacacaggt agccgatcgc ttgcattttg
     2941 ccatccggta gtctgaaatc cggaaagctc tttttttcag cgctgtcaga ccaattgccc
     3001 agtcttttat tccagagatt tctccagctt ttcagggttg gccccagatt ttttaaacct
     3061 tgcagggaaa acaaatctgc tccgaggggg atggcgacat aatcggtcgc aatcaggacc
     3121 gaccggttga tggcgcccag attaggcccg atatccacca gaacgatgtc tgcctgcact
     3181 ttgctggccg ccatttgcgt cacctgccag aatgcagtca gaatgcgtag aggacggtaa
     3241 aggttgctat cgttcatact gccaggccac gcagcggaaa gctcatcctc aaaactggac
     3301 aggttgacgt cgcccggtag cagatacagg tcggttgcaa tattctggag aacaggttca
     3361 gcgatgtcgc cgacgcccat caggggcttg atgcattgat aaatagttga acctgtattt
     3421 tcttgcttcc agatttcctc gatctggttt tcatcgagaa aagccgctgt cagattggcc
     3481 tgtggatcaa gatcgatgat caccacccgc ttgtgcaata gcgcaaacat ccaggccagg
     3541 tggtaaatca gagaggtttt gcctacgcct cccttgttat tgaaaaaggt cagtacggaa
     3601 gtactcatga ttttcctcgc aatatgcaga ccacggcgct ttgactggtg ttaaattctg
     3661 gcgcggggtt gccattcaac cccattgcct ggcgggagat ggcgatgccg cgaccaaaag
     3721 cctggatgaa tccaaaggtt ttcagtacgt ccgcgatgtt cggattacgg taatcggtga
     3781 tgcccggttt gccgaaattc tctgctgtga cattgccata aggaccgccg gggctgttga
     3841 tctcgatgcg atcattaaac caatacaccc gtatgggggc gttggtactc tcataggtac
     3901 ggtgcagtac agcgttgtag aggacttgct gaatggcgat ctgggggtaa ggcatgtcta
     3961 cctgatgtgt cgccgccgag gtgatatcga cggcagcacg attgtgtgct ttcaactttt
     4021 cttcagttct gcggagtata tcaacgatgc cgccaccgat ttctgcttcg tcaatgaccg
     4081 ggtcagacag ttcgatgccg tcgatacgca agaattgaac ataagcgcca ggcaagaaat
     4141 cttgtggtcg tttgctgatc gctagcatgc ctgtgatagt gggtgtggtg tcgtccggcg
     4201 cgacgatcat gcggcaggaa gcgagccgtt cttcataact gcggccattg gcctccagaa
     4261 cgtcaggagc aaacgcttgc ggcaggtatt cattctcaaa aatgacccga gagagatcgc
     4321 ttaacctggc ggaaggcaat ggatggatgt caaacggcag gtttttgtaa cgtcgttttt
     4381 cattcagaat acgctcttcc tgttcgttag ccagtgagcg gcgcggacca gtgcgaatcc
     4441 agatgcgccc gtcgtatttt agtgggggca tgtcggatgg catcaccgtc acgacggtta
     4501 tctcagatcc tttcaagatg cgtttttctg ctgaaagcac tggcaggggc agaatattac
     4561 cgtctgtttt catatcggca agcgatagca gaagctagtc cgttatttcc aggccgctgg
     4621 gtttgccgtc atcttttgcg ccgataaaca aaacacctgg ccggttatgt cctggtaaat
     4681 cattggcaaa ggcgcatact gtctggcgcg cttttttggt gatgtcccct ttaaaagtct
     4741 ctttgcgctc ggtacggtca gactccgtgt cgtcgagcaa atcgagtagt tcttgatcag
     4801 tcaatctttg cattttgctg ccccggaata agagtccaca tgatatgacg cggatacagt
     4861 atcgcaccag cccgatgatg ggtgtagaat ccggcccaca acatttgaaa ctgaaggaag
     4921 taacgccggg gtcctggatg ccgatgccat ggcgcgcggg tgagaaatat ccctggacct
     4981 gatccaggta atgccagcat cagtcaaagg agccaaaatg aatgtcaatc cgaaatttct
     5041 gtccgctact gctacggtgg atgaggcagc ggttcagcct ttaccgaatt cccgcaagat
     5101 ttacgtcact ggctcgcggc ctgacatccg tgtgccgatg cgtgagatca gccagtccga
     5161 tacgccggcg atgttcggca cggaaaagaa tccgcctatc tatgtgtatg atacttccgg
     5221 cccgtacacc gatccggagg tcaggatcga cattcgcgcc ggcctggcga cgccgcgcct
     5281 gccgtggatt ctggagcgca acgatactga agaactggca ggtccgactt cggagtacgg
     5341 tcagcagcgc ctgaatgatc cggggctggc cgagctgcgc ttcaagctgc gtcgcaagcc
     5401 gcgccgcgcc agggcgggcg gcaatgtcac gcagatgcac tatgcgcgtc gcggcatcat
     5461 cacgccggaa atggaattca tcgccatccg cgaaaatctg cgccgccagg aatatctgga
     5521 gcaacttcgg gcctctggcc cgatgggcgg caagctggcc gaccagatgg gccgccagca
     5581 tcctggccag tcgttcggcg cgtcgcttcc cgccgtgatc acgccggaat tcgtgcgcga
     5641 cgaagtcgcc cgcggtcgcg ccatcattcc cgccaacatc aatcatccgg aagtcgagcc
     5701 gatgatcatc ggccgcaact ttctggtgaa gatcaatgcc aacatcggca actcggcggt
     5761 gacgtcgtcc atcggcgaag aagtcgaaaa gatgacctgg gcgattcgct ggggcggcga
     5821 caatgtgatg gatctgtcca ctggcaggaa catccacgaa acacgcgaat ggatcatccg
     5881 taattcgccg gtgccaatcg gcacggtgcc gatttatcag gcgctggaaa aggtcaaagg
     5941 caaggccgag gatctgacct gggaaatctt ccgcgatacg ctgatcgagc aggccgagca
     6001 gggtgtcgat tacttcacca ttcacgccgg tgtgcgtctg caattcgtgc cgctgacggc
     6061 caggcgcatg acaggcatcg tctcgcgcgg tggttccatc atggcgaagt ggtgtctggc
     6121 gcatcacagg gaatccttca tttacgagca cttcgaagac atctgcgaaa tcatgaaagc
     6181 ctacgacgtc agcttcagtc ttggcgatgg cttgcgtccg gggtcgatct atgatgccaa
     6241 tgacgaagcg caactgggcg agctgaagac gctgggcgaa ctgaccagga tcgcgtggaa
     6301 gtacgatgtg caggtgatga tcgaaggtcc aggtcatgtg cctatgcatc tgatcaagga
     6361 aaacatggac ctgcaactgg agcagtgcca tgaagcgcca ttctatacac tgggtccctt
     6421 gaccactgac atcgcgccag gttacgacca catcacttcc ggcatcggcg cggcgcagat
     6481 cggctggtat ggtacggcga tgctgtgcta tgtcacgccg aaggagcatc tgggcttgcc
     6541 gaacaagacg gatgtgaagg acggtatcat cacctacaag attgctgcgc atgccgctga
     6601 tctggccaag gggcatccgg gcgcacagat tcgcgacaat gcgctgtcga aggcgcgttt
     6661 cgaatttcgc tgggaagatc agttcaatat cggtctcgat ccggacaagg cgcgtgaatt
     6721 ccatgatgaa accttgccca gggatgcggc caaggtggcg catttctgtt cgatgtgcgg
     6781 tccgcatttc tgctcgatga agatcacaca ggaagtacgc gattatgcgg ccagggaagg
     6841 catcccggaa accgatgcgc tcaggcaagg catggcagtg aagtcggtag agttcgtcga
     6901 aatgggagcc gaaatttatt ccagggcttg agaatgtgtt gacattctga ggcagaggtg
     6961 aatgcgatgc agattgaatt gaacggcaag ccgcatgtag tggccgaggg ggcggatctg
     7021 gcgcagttga tcgcggcgct ggatctgacc gggaaggcgg tagcggtagc agtgaaccgg
     7081 caggtggtgc cgtcgcagtc gtgggcgggg cggatgttgg tggagactga tcgggtggat
     7141 gtggtgcggg cgattggcgg tgggtaaagt gaagatgagc gatctacccg cgctgtcgac
     7201 agacgcagga ctgacgattg ccggtgagac ctacggttcg cgtctgctgg tcggcagcgg
     7261 caagtacaca gacctcgacg aaatgcgcgc cgctaccgaa gccagcggtg cggatatcat
     7321 cacggtggcg attcgccgtg tgaatatcgg gcaggatccg catgcgccga gtctgctcga
     7381 tgcgctgccg cctttgcgct ataccatcct gccgaatacg gccggttgct ataatgccga
     7441 ggatgcggtt tataccttgc aactggcgcg tgaactgctg catggacaca agctggtcaa
     7501 actggaggtg ctgggcgatg agaaaagttt gttccccaac atgccggaga ccctgaaagc
     7561 tgccgcgatg ctggtcaagg atggtttcga tgtgatggtg tattgcagcg acgatccggt
     7621 gcaggcacgc atgctggaag atctcggcgt ggcggcgctg atgccgctgg cgtcgctgat
     7681 cggttccggc atggggattc tgaatccgtg gaatctgtcg ctgatcatcg aacagtcgaa
     7741 cattccggta ctggtcgatg ccggtgtcgg caccgcttcc gacgcggcga ttgcgatgga
     7801 acttggctgt gctggtgtgt tgatgaatac cgcgattgcc ggcgcgcgcg atccggttcg
     7861 catggcgcgg gcgatgaagc tggcggtggc agcggggcgt gaagctttct tggctggccg
     7921 tattgcgaag cgttttgccg cgtcaccatc gtcgccgatg gccggccgca tgacgtgaag
     7981 caggcgctgc gtgcttatcg cacgtcgccg ccgtgcgtac tggttttttc cggttccgat
     8041 ccgagcggtg gcgctggcat gcaggcggat attcccgcta tcagcgcgct gggttgtcat
     8101 cccctgtcgg tgccgacggc gctgacggtg caggataacg tccgtgtgtt cgcggtgcat
     8161 gcgctggagg cggaattgat ccgccatcag gcgcaggtat tgatcgaccg ttttcagatc
     8221 agtgcggtca agctcggtat cgccggtaac cgtcgcaatg cggcaatgat tgctgaactg
     8281 attctgcaat tgcgtgcgcg tcaaccgggg ttgccggtgg tgttcgatcc ggtgctggcc
     8341 aatggcaagg gcgactgcct gtcgagcgac gatgcggcgc tggcgctgga gccgttgtat
     8401 gcggtcgcta ccgtgatcac gcccaatctt gacgaagccc atcgtttatg cggcagcgaa
     8461 catgcgccgg agcagcaggc tgccttgctg atggcacgcg gttgcgggca tgtcttgctc
     8521 aagggcggtc atggaccgca acatcaggat gtgatcaatc gctggtttgg ccctgatggt
     8581 gagggtgcat cgtggcgctg gccacgtttg ccggatgcat ttcacggcag cggctgtacg
     8641 ctggcggcgg ccttgtcggc gcaactggcg cacggtatcg acatgcgcgc ggcaatcgaa
     8701 gcggcacagc gctattgtca gcagacgctg gaaacttcct atgcgattgc ggcagggcag
     8761 cgcattcccg atcgtgttcc tttgtttgca ggagaaaacg gaccgcgcgg gattattctt
     8821 gaaacatgaa caggtgaaaa aatgcggctt ggctttttag gtgcggggac aatcagtaat
     8881 gctgttattc gcggcatttg caggggcgat atggccgagg cattaggcat cgaggtgatt
     8941 tttgtctcac agcgcaatgc acagatagca gccaagctgg caaaagattt tgcctgcata
     9001 aaaattactg aagataatca gcagctggtg gataatgccg atctggtttt tgttgctctg
     9061 ccgggggctg tttgtgagca ggcgctgcgg gcgctgcaat tcagggccga tcaaaagctg
     9121 atttcttttg tgccaaaccc aagctgcgca gaattggcgg aatggacggg gggcaaactg
     9181 ccggttttac gtgccgtgcc tctgcccttt atagcggagc ataaaacggc tacgcccatt
     9241 ttccccgcgg ataaagattt gcaggcattg tttggcgcga caggcggctg cattcttgca
     9301 gaagaggaaa agcagtttaa tttcttcatg acagccggtt ccatgatggg ggtctattac
     9361 cgtttttgcg ggctttgcac gtcttggctg agccaaagag gtttaccgga aaaacaatcc
     9421 atgctttaca tgacgcggct tttcagagca ttgtctgaag aagcggtgaa aggaaaggat
     9481 tttgctgctt tgcagcagga atattccacc aaaggcggca caaacgaact gatagccaaa
     9541 ttatttgagg ataaaggcgg cgtagaagcg ctgcttgccg cgctagaaga agggtttgcc
     9601 cgcattaacg gcaaataaaa gccatagtgc tgcaaatcta ttacgtatct aatcaatgaa
     9661 tgacgccagc aggcaatggc atttgcgcgg cctgtacatc gtgacgcccg attgggacga
     9721 tacggaaaaa ctggtcaacg tgacagcgca ggccatcgaa ggcggcgcgg cgctggtgca
     9781 gtatcgtcac aagactgcgg ctgccgcctt gcgtgcagaa caggcgggcg cgctgctgaa
     9841 aatctgccgt cgctgcaata cgccgctcat catcaatgat gatatcgatc tgtgtctggc
     9901 gctcgatgct gacggtattc acgtcggcgg cgccgatgcg ccggtggcgc aagcgcgggc
     9961 aaaggtcggt cgggacagga tcgttggtgc gtcgtgttac ggcgccctgc aactggcacg
    10021 tgacgcgcat gtggcaggag caagctatgt ggcgttcggc ggtttttatc cgtcgcgcgt
    10081 gaagcagtac ggttttagga cggcaccggg aatcgtggcg gagtcgaaaa aagaaattcc
    10141 cttgccggtc gtcgtcatcg gcggcatgac gcaagagaat tgcctgccgt tgatcaggca
    10201 gggcgccgac atggtggcgg ccatcagcag tatatacatg acggaagaag cggcgcggga
    10261 agcggcgtgc cgctttgcgg gtttgttttg aggcaaaccc gctgacggcg cggacgtata
    10321 attgcgggat gcaaaatctt cttcagcatc tcaacgacgg acagctcgcc gccgtcaccc
    10381 tccctgccca atctgccttg atcctggccg gcgccggctc cggcaagacg cgcgtgctga
    10441 cgacgcgcat cgcctggctg atccagaccg gtcaggtatc gccatcgggc attctggcgg
    10501 tgactttcac caacaaggcg gcgaaagaaa tgctcacgcg cctgtcggcg atgatgccga
    10561 tcaatccgcg cggcatgtgg attggcacct ttcatggatt gtgcaatcgt ctgctgcgcg
    10621 cacaccatcg tgacgccgcg ttgccgcaga ccttccagat tctcgataca caggatcagt
    10681 cgtcggcgat caagcgttta ttcaagacaa tgaatatcga tgacgaaaaa tatccgccgc
    10741 gcgatctgat gtacttcatc aacggcgcca aggagcaggg tctgcgcgcc ggagatatcg
    10801 atacgcatga cgattacaac cgcagattcg tcgaactcta cgacctgtac gaccagcagt
    10861 gccagcgtga aggtgtggtc gattttgccg aactgttgtt gcgcacctat gaactgctat
    10921 cgcgcaatca gccgctgcgt gagcattatc agcgccgctt caggcatgtg ctggtggatg
    10981 agttccagga taccaacgac ctgcaataca aatggctcaa gttgatatcc gggcagggcc
    11041 ggatcgacag cagcgcggta tttgccgtcg gtgatgacga ccagagcatt tatgcttttc
    11101 gcggcgccaa cgtcggcaac atgtctgcct tcgagcgtga gttccgcgtc gggaacctga
    11161 tcaggctgga gcagaattac cgctcgcacg ggcatatcct ggatgttgcc aatgtgctga
    11221 tcgccaacaa cagcaggcgt ctcggcaaga acctgcgcac ggatgccggc ctcggcgaac
    11281 aggtgcgtgt gttcgaagca gacagtgatc tgcaggaggc gcaatggctc atcgagcagg
    11341 tcagggatct gatcggccaa ggctggatgc gcagtgaaat cgccgtactc tatcgctcca
    11401 acgcccagtc acgcgtgctg gaacgcgcct tgttttcggc ggcgatcgcc tatcgcgtgt
    11461 atggcggcct gcgcttcttc gagcgcgccg aaatcaagca cgcgatcgcg tatctgcaat
    11521 tgctggataa cccgcacaat gattcagctt ttctgcgtgt ggtcaatttt ccggcgcgcg
    11581 gcatcggcgc cagaacgttg gagcaattgc aggatgcggc gcgccagtac gactgttcgc
    11641 tctacgccgc cgtgccttac gtcgccggca aggctggttc cgcgctccgt gctttcatca
    11701 aactgatcga aggcgtgcgt tgcgggacgc agcatctgcc cttgccggaa atgctgcagg
    11761 tcgtgctcga tgccagtgac ctgatgacgc attaccagaa cgagaaggac ggcgacggcc
    11821 gcatcgaaaa tctgaaggaa ctcatcagtg acgcgaccca attcgtgtcg gcagaaggct
    11881 atggtctcga cacgccggcc ttctccggtc cgcaagcgcc ggctgttgcc ggggcatcca
    11941 tcacgacggc tgacggtatc gacatcatcg acgccgatgc catgctgacg gcgctcatgt
    12001 cgccgctgtc ggccttcctc tcgcatgctt cgctggaagc cggcgacaat caggcacagg
    12061 ccgggcagga tgcattacaa ctgatgacgg tacatgcggc caagggactg gagtttgatg
    12121 ccgtgttcat caccggtctg gaagaaggct tgttcccgca tgacaacagc gcgaaggaag
    12181 atggcggcgt ggaagaagag cgccgcctga tgtacgttgc catcacgcgg gcgcgcaagc
    12241 gcttgtacat gagtttttcg cagacgcgca tgttgcgtgg tcagacgcgc tatcacaggc
    12301 gttcccgctt tttcgatgaa ttgccggatg cgtcgctcaa gtggctgtcg ccgaagattc
    12361 aacagcatag ctggttcgcg catccgaaag ccgcctggga cgatgtgtct gagtccggta
    12421 acaacaggat tgcgcagaat ttccgccggt ctgatacagg gtggcgtatc ggtcagaatg
    12481 tcacgcatgc caggtttggt gaaggtgtga tcgtgaatat cgaaggcacg ggcgttcatg
    12541 tacgggcgca catcaacttc ggcaagtctg gcatgaagct gctggatttg agtgtggcga
    12601 agctggagcc ggttggttga tgctctggtc ttgcgacgcg ccacccgcac gacat
//
LOCUS       DASRDH010000141         3473 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_420046,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000141 DASRDH010000000
VERSION     DASRDH010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 3473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3473
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420046"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2664
                     /gene="mfd"
                     /locus_tag="VFA14_03215"
     CDS             <1..2664
                     /gene="mfd"
                     /locus_tag="VFA14_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HZS80425.1"
                     /translation="APAGIEYYLPLFFEQTATLFQYLPRDSLFATVGDIDQAIKRFWN
                     DTRTRHHFLEADRERPVLAPEQVFLTDEDFFTLVKPYGRLALQGDDAPSAWSAPLPDV
                     AVNRHSEDPLTNLRAYLFKTGHRVMLCAESNGRRETLQQYFREYDLHPALCDGYDSFI
                     AGNAPLMLGIAPLHAGFELTGAALSFITETELYAGSGRRAGHRKQEALTQVEYMVRDL
                     SELKIGDPVVHAYHGIGRYMGLLSMDLGEGETEFLHLEYARETRLYVPVSQLHVISRY
                     SGAAPEDAPLHVLGSGQWEKARRKAARQVRDTAAELLDLYARRATRQGHAFHYSVRDY
                     EAFADSFGFEETPDQDAAIRAVIGDMTSGKPMDRLICGDVGFGKTEVALRAAFVAVMG
                     GRQVAILAPTTLLAEQHAQTFTDRFAGWPVRIAELSRFRTGKEVMQAIEGMADGTLDI
                     VIGTHKLLSGDIKFSRLGLVIIDEEHRFGVRQKETLKSLRAEVDVLTLTATPIPRTLG
                     MALEGLRDFSIIATAPQKRLAIKTFVRAEDESTIREACLRELKRGGQVYFLHNEVETI
                     ENRKAMLEALLPEARVVIAHGQMHERDLEKVMRDFVAQRSNILLCTTIIETGIDVPTV
                     NTIIMHRADRFGLAQLHQLRGRVGRSHHQAYAYLLVNDVKGLTRQAQRRLEAIQQMEE
                     LGSGFYLAMHDLEIRGAGEVLGDHQSGEMHEIGFQMYSDMLNEAVRALKSGKEPDLTA
                     SLTSATEINLHVPALLPGDYCGDVRERLSLYKRFANGASQEAIDALQEELIDRFGKLP
                     EAARALVETHRLRIAARPLGIIKIDAHAESAVLQFVPNPPVDGMRIIELVQKNRHIKL
                     NGQDRLRITAGMPDLNARVTQLKATMRALAG"
     gene            complement(2675..>3473)
                     /gene="miaA"
                     /locus_tag="VFA14_03220"
     CDS             complement(2675..>3473)
                     /gene="miaA"
                     /locus_tag="VFA14_03220"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HZS80426.1"
                     /translation="AKPNASELASVPHHLIDILDPSAAYSAMQFRHDALRLAQEIRAR
                     GKLPLLVGGTMLYFKVLRNGLDDLPEGNPSVRARLDEEAARGSVPAQHARLAALDPKT
                     AARLNPNDNQRIQRALEIIELSGRPMSALLVQKDPQTLPFGLLALTLEPSDRTVLHQR
                     IARRFDLMLENDGLLEEVRRLRARGDLHLGLPSMRCVGYRQVWEYLDGQYGYTEMRER
                     GIIATRQLAKRQITWLRSTPDRVIIDCNAADATQIALNHVYTAINHQ"
BASE COUNT          671 a          979 c         1090 g          733 t
ORIGIN      
        1 gccccggccg ggatcgagta ctatctgccg ctgttcttcg agcagaccgc aaccctgttc
       61 cagtatctgc cccgcgacag cctgttcgcc acggttggcg acatcgatca ggccatcaag
      121 cgtttctgga acgacacgcg cacgcgccat cattttctcg aggccgatcg tgaacgtcca
      181 gtgctggcgc cggagcaggt cttcttgacc gatgaagatt ttttcacatt ggtgaaacca
      241 tatggtcgtc tagcgctgca aggcgatgac gcaccgtcgg cttggtccgc gccgcttccc
      301 gatgtcgccg tcaaccgtca cagcgaagat ccgctgacca atctgcgcgc ttatttgttc
      361 aagactggtc atcgtgtgat gctgtgtgct gagtcgaatg gccgtcgcga aaccttgcag
      421 cagtatttca gggaatacga tctgcatccg gcattgtgcg atggttacga cagcttcatc
      481 gccggtaatg cgccgttgat gctgggtatc gcccctttgc atgccggttt cgagctgacc
      541 ggcgccgcct tgagtttcat caccgagacg gagctttacg ccggttccgg tcgccgcgcc
      601 ggtcacagga agcaggaagc gctcacgcag gtcgagtaca tggtgcgcga tctgtccgaa
      661 ctcaagatcg gcgacccggt ggtgcatgcc tatcacggca ttggccgcta catgggtttg
      721 ctcagtatgg atctgggtga gggcgaaacc gagtttctgc atctcgaata cgccagagaa
      781 accagactct acgtgccggt gtcgcaattg catgtgatct cgcgctactc aggcgcagca
      841 ccggaggatg cgccgctgca tgtgcttggc tccggccagt gggagaaagc caggcgcaag
      901 gctgcccggc aggtgcgcga tacggccgcc gaattgcttg atctctatgc gcgccgtgcc
      961 acgcgccagg ggcatgcgtt tcattattcc gtgcgcgatt acgaagcctt cgccgacagt
     1021 ttcggttttg aagaaacacc cgatcaggat gccgccatcc gcgctgtcat tggtgacatg
     1081 accagcggca agccgatgga tagactgatt tgcggcgatg ttggcttcgg taaaacggaa
     1141 gtcgccttgc gcgcggcttt tgtcgctgtc atgggcggca gacaggtggc gattctagcg
     1201 ccgacgaccc tgctggcgga gcagcatgcg cagacgttta cggatcgttt cgccggctgg
     1261 ccggtgcgca tcgccgagct gtcgcgtttt cgcaccggca aggaggtcat gcaagccatt
     1321 gagggcatgg ctgacggcac gctcgatatc gtcatcggca cgcacaagct actgtccggc
     1381 gacatcaaat tctcacggct cggcctggtc atcatcgatg aagaacatcg tttcggcgtg
     1441 cgccagaagg aaacgctcaa atccttgcgc gccgaagtcg atgtcttgac gttgaccgcg
     1501 acaccgattc cgcgcacgct gggcatggcg ctggaaggtt tgcgcgattt ctccatcatc
     1561 gccaccgcgc cgcaaaagcg gttggcgatt aaaaccttcg tgcgcgctga agacgaatcg
     1621 accattcgcg aagcttgttt acgcgagctg aaacgcggtg ggcaggtcta ttttctgcac
     1681 aatgaagtgg aaaccattga aaaccgcaaa gccatgctcg aagccttgct gccggaagcg
     1741 cgtgtggtca tcgcgcacgg ccagatgcac gagcgcgatt tggaaaaagt catgcgcgat
     1801 ttcgtcgcgc agcgttccaa tatcctgctc tgtacgacga ttatcgaaac cggcatcgat
     1861 gtaccgaccg tcaacaccat catcatgcat cgcgccgatc ggttcggtct ggcgcagttg
     1921 caccagttgc gcggtcgggt cggtcgctca catcaccagg cttacgccta tctgctggtc
     1981 aatgacgtca agggtctgac caggcaggca cagcgccgtc tggaagcgat ccagcagatg
     2041 gaagaactgg gcagcggctt ctatctcgcc atgcacgatc tcgaaatccg cggcgccggc
     2101 gaagtgcttg gcgaccatca gtcgggcgag atgcacgaga tcgggttcca aatgtattcc
     2161 gatatgctca atgaagccgt gcgagcgttg aagagcggca aagaacccga tctgaccgcg
     2221 tccctgacga gcgcgaccga aatcaatctg cacgtaccgg ccttgctgcc gggcgattat
     2281 tgcggcgatg tccgcgagcg tctctcattg tacaaacgtt ttgccaatgg tgcgtcgcag
     2341 gaagccatcg atgctttgca ggaagaattg atcgaccgtt tcggcaagtt gcccgaagca
     2401 gccagggcat tggtggaaac gcatcgtctg cgcattgccg ccaggccgct gggtatcatc
     2461 aagatcgacg cgcatgccga gtcggcggtg ttgcaattcg tgccgaatcc gccggtcgat
     2521 ggcatgcgta tcatcgaact ggtgcaaaag aatcgccata tcaagctcaa tggtcaggac
     2581 aggttacgca tcaccgccgg catgcctgat ctgaacgcaa gagtgacgca gctcaaggcg
     2641 accatgcgcg cgctggccgg ctgatgagaa cctatcattg atgattgatt gctgtgtaca
     2701 cgtgattcag cgctatttgc gtcgcatcgg cggcattgca gtcgatgatt acccggtcag
     2761 gcgtggagcg taaccaggtg atctgacgtt tggccagttg gcgggtggcg atgatgccgc
     2821 gttcgcgcat ctcggtgtag ccgtattggc catcgagata ttcccagacc tggcggtagc
     2881 cgacgcagcg catcgacggc aggccgaggt gcaggtcgcc gcgggcgcgc agtctgcgga
     2941 cttcttcgag caggccgtcg ttttccagca tcaggtcgaa gcggcgggcg atgcgttggt
     3001 gcagcacggt gcggtcggat ggctccagcg tcagtgccag caggccaaag gggagggttt
     3061 gcgggtcttt ttgtacgagt agcgccgaca tgggtctacc gctcagttca atgatttcca
     3121 atgcgcgctg gatacgctgg ttatcgttgg ggttcaggcg ggcagcggtt ttcggatcga
     3181 gtgcggctag gcgtgcatgt tgcgccggca cgctgccgcg cgcggcttct tcatcgaggc
     3241 gggcgcgcac ggagggattg ccttcgggca agtcgtcgag accgttgcgc aataccttga
     3301 aatacagcat ggtgccgccg accaagagcg gcagtttgcc gcgtgcgcgg atttcctgtg
     3361 cgagacgcag ggcgtcgtga cggaattgca tggccgagta ggctgcgctc gggtcgagga
     3421 tgtcgatcag atgatgcggc acgctggcaa gctcgctggc attgggcttg gcg
//
LOCUS       DASRDH010000142         3129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1365054,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000142 DASRDH010000000
VERSION     DASRDH010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 3129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3129
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1365054"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(277..648)
                     /locus_tag="VFA14_03225"
     CDS             complement(277..648)
                     /locus_tag="VFA14_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS80427.1"
                     /translation="MKFSIRLCGIAVLSMSLIACTIGNGRICGPQTPRAYCDKEAYEK
                     LLHPKAYGEYWVKPGMTKENWREDWVGCGGYKNGNFSPHVRDINTLIEQGLSQSEARE
                     KLKDSLKSCMQSRGYEYLREP"
     gene            complement(645..2186)
                     /locus_tag="VFA14_03230"
     CDS             complement(645..2186)
                     /locus_tag="VFA14_03230"
                     /inference="COORDINATES: protein motif:HMM:NF024728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
                     /protein_id="HZS80428.1"
                     /translation="MFNLASAVTWTNTHVHAGKQVAMTSGSDTTLKGAVVSAPQITAD
                     VGGDLHIASLQDTSRFDSKQQNIGGSISAGHGKMSDSLSVGKSNTDSNYASAQEQSSL
                     RAGDQGFDVNVANNTDLKGGAITSTQKAIDDNKNNFSTGGELTTNDIKNQASYKASSV
                     GVNISAGTSLDGKLAPQGTGAGIGADSGNAGSTTQATISGVAGNKDARTGDAETGIAK
                     IFDADKVQKDIDAQVQITQEFGRAAGKLVSDDAQNQRKDLQERLKQADETDRQAIQAQ
                     LEDVTMQERVMNVLIGAVSGFGGTALTRESLSAAAGQMRQLMIEDSQRFAGVTDRTTT
                     LSNVSGASEGMREDGFKIGGTRIDLDLMCGVDNKRCLRNEDGSLRLNKNGQVQFDPKS
                     ADGKSLAEFIETTEEGKKMSGLTGGIQGWKGTLFGIPYPSGSWPDKLVEAFSGTHDMV
                     GGKLSGLYDEQGNATRDRGTMEKTAQEAWSATGAIVASAPFAMAEFLSPEVWKAIAIL
                     LGAAK"
     gene            complement(2199..2570)
                     /locus_tag="VFA14_03235"
     CDS             complement(2199..2570)
                     /locus_tag="VFA14_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS80429.1"
                     /translation="MKFSIRLCGIAVLSMSLMACTIGNGRICGPQTPRAYCDKEAYQK
                     LLHPKAYGEYWVKTGMTKESWREDWVGCGGYKDGDFSPHVRDINTLVEQGLSQSEARE
                     KLKDSLKSCMQSRGYEYLREP"
     gene            complement(2593..2976)
                     /locus_tag="VFA14_03240"
     CDS             complement(2593..2976)
                     /locus_tag="VFA14_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS80430.1"
                     /translation="MESDIDFIERRNMKNLLRGFVVLSLCVVISSCELLCQAGTGTCG
                     MSDEQMDKFLHPKTYGEYFVKPGMTKEGWRADWVACGGDPSGQHGVYTPPGSTTQVVL
                     AAQNREADRLAICMQSKGYEYRSEP"
     gene            complement(2937..>3129)
                     /locus_tag="VFA14_03245"
     CDS             complement(2937..>3129)
                     /locus_tag="VFA14_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS80431.1"
                     /translation="GGKVTGLYDEQGNIRRGMTKAERRVYDYAVTPAALLPSTPFATA
                     ELLSPEIWKAISILLKGAI"
BASE COUNT          593 a          909 c          750 g          877 t
ORIGIN      
        1 tgggcgctgg tcgccgttgt ggtggatgtg atgaaattgg cgtcgcgtcc agtcagcacg
       61 gcgatacggt tgccgttgag cttgccgctg ctgttgaggt cgttggccgc tatcaatgcc
      121 gtcgtgccag tttgcgtatt gctttggatg acgccgctgt tgttgatatc gccggtgatc
      181 tcctgctctc aatcctgact gtccgctcac gctggcgtaa tcgcgccgac gctgaggttg
      241 ccgtggacca gccgagatac tgccgccgat acatcatcac ggctctctga gatattcata
      301 gcctctggat tgcatgcatg acttcaaaga gtctttcagt ttttctctgg cctcggattg
      361 tgacagcccc tgctcaatca aggtattaat gtccctgacg tggggactaa aattaccatt
      421 cttatatcct ccacagccca cccaatcctc ccgccagttt tctttcgtca tgccgggttt
      481 cacccaatat tcgccgtagg ctttggggtg caggagtttt tcatacgcct ctttgtcaca
      541 gtaggcacgt ggagtttgcg gtccacaaat tcttccattg ccaatcgtgc aggctattaa
      601 cgacatactg agaaccgcta tgccacataa tctgatcgag aatttcattt cgccgctcct
      661 aacaaaatag caatggcctt ccatacttct ggtgacaaaa attctgccat cgcaaaaggt
      721 gcagacgcaa caattgcgcc tgttgcgctc catgcctctt gcgctgtttt ctccatcgtg
      781 ccgcggtccc ttgttgcatt accttgctca tcatacaatc cgctcaactt gccgccaacc
      841 atgtcgtgtg tgccgctgaa agcttcaacc agtttgtcag gccaactacc ggaaggataa
      901 ggaataccga acagcgtacc tttccagcct tgaatgccgc cggtcaggcc agacatcttt
      961 tttccttctt cagtggtttc aatgaattcc gccagggatt tgccatcggc ggatttgggg
     1021 tcgaattgca cttgaccgtt tttatttaat cgcaaactcc catcctcatt cctcaagcac
     1081 cgcttgttat ccaccccaca catcaaatcc agatcgatgc gcgtgccgcc gatcttgaag
     1141 ccatcctccc gcatgccttc gctcgcgccc gatacattgc tcaacgtggt tgtacgatct
     1201 gtcacaccgg caaaccgttg cgagtcttcg atcatcagtt gacgcatctg cccggcagcg
     1261 gctgacaagc tttctctggt cagcgctgtg ccaccgaagc cgctgaccgc gccaatcagc
     1321 acattcatca cccgttcctg catcgtcacg tcctcgagtt gcgcctgtat cgcctgcctg
     1381 tctgtttcat ccgcctgttt gagtcgttcc tgcaaatcct ttctttgatt ttgcgcgtca
     1441 tcactgacca gcttgcccgc ggcgcgtccg aattcctgcg tgatctgcac ctgtgcatcg
     1501 atgtctttct gtaccttgtc agcgtcgaag attttggcga tgccggtttc tgcatcgcca
     1561 gttctggcat ccttgttgcc agcgacaccg ctgatggttg cctgcgtggt gctgcctgca
     1621 ttgccgctgt ctgcgccaat ccccgcaccg gtgccctgcg gcgcgagttt gccgtcgaga
     1681 ctggttcccg cgctaatgtt gacgccgacg ctgctggctt tgtagctggc ctggtttttg
     1741 atgtcgttcg tggtgagttc accaccggtg ctgaaattat ttttgttgtc gtcgatggct
     1801 ttttgcgtgc tggtgatcgc gccgcctttg aggtcagtat tgttcgctac attgacgtcg
     1861 aagccttgat ctcctgctct caggctgctt tgttcctgtg cgctggcgta attgctgtct
     1921 gtgttacttt tgccgacgct gaggctgtcg ctcatcttgc cgtgaccagc cgagatactg
     1981 ccaccgatgt tttgctgttt gctgtcgaag cggctggtat cctgaagcga tgcgatgtgc
     2041 aggtcgccac ccacatcagc agtgatctgc ggtgcgctga ccaccgcgcc tttgagcgtg
     2101 gtgtcgctac ctgaggtcat ggctacctgt ttgccggcat gcacgtgggt gttggtccac
     2161 gtcactgccg atgcgaggtt aaacaccact gtttatcatc acggctctct gagatattca
     2221 tagcctctgg attgcatgca tgacttcaaa gagtctttca gtttttctct ggcctcggat
     2281 tgtgataacc cctgctcaac caaggtatta atgtccctga catgaggact aaaatcacca
     2341 tccttatatc ctccacagcc tacccaatcc tcccgccagc tttctttcgt catgccggtc
     2401 ttcacccaat attcgccgta ggctttgggg tgcaggagtt tttgatacgc ctctttgtcg
     2461 cagtaggcac gtggagtttg cggtccacaa attcttccat tgccaatcgt gcaggccatt
     2521 aacgacatac tgagaaccgc tatgccacat aatctgatcg agaatttcat ttcgctgctc
     2581 ctaacaaaat tatcacggct cgctacgata ttcatagccc ttggattgca tgcagatggc
     2641 gagacgatcg gcttctcggt tttgggcagc gagaaccact tgcgtggtag atcctggagg
     2701 cgtgtacaca ccatgctgcc cgcttggatc tcccccacat gctacccaat ctgcacgcca
     2761 accttctttc gtcatgccgg gtttcacaaa atattcgccg taggttttgg ggtgcaggaa
     2821 tttatccatc tgctcatcgc tcatgccaca ggttcccgtt ccagcctgac aaagcagttc
     2881 gcagcttgat atcacaacgc atagagacag cacaacgaaa ccacgtaaaa gatttttcat
     2941 attgcgcctt tcaataaaat cgatatcgct ttccatatct cgggcgatag cagttccgca
     3001 gtcgcaaagg gtgtgcttgg caagagggcg gcgggggtta ccgcatagtc ataaacacgg
     3061 cgttcagcct tcgtcatccc tctcctgata ttgccttgct cgtcgtacaa accagtgact
     3121 ttcccacca
//
LOCUS       DASRDH010000143         2437 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1614653,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000143 DASRDH010000000
VERSION     DASRDH010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1614653"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(75..986)
                     /locus_tag="VFA14_03250"
     CDS             complement(75..986)
                     /locus_tag="VFA14_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HZS80432.1"
                     /translation="MSSPIDLTQLPAPNIIESLDYEQILANRKAKLLTLVPEDRRNEI
                     EATLQLESEPLTILLEENALQEMVLRNRINDAARAVMLAYAKGADLRGIGANFNIEQL
                     IVTPADPDAVPPVAEVREDEESLKLRIQEAFDGLSVAGPGAAYEFFARSAHGQVADAR
                     AASPAPCEVVIAVLSTEGDGTADQALLDAITAALRHEDIRPIGDLVTVQSAEVIPYEI
                     VAEIYVPPGPESSIISDAAIQRAQDETEKKRPLGRGVYRSKLDAVLHAEGVIKVNLIE
                     PSTDIVLTSQQAAYCTNITVTTKVMDV"
     gene            complement(983..1306)
                     /locus_tag="VFA14_03255"
     CDS             complement(983..1306)
                     /locus_tag="VFA14_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HZS80433.1"
                     /translation="MNAASGRAISGVDHLRQSMADILNTPIGSRVMRRTYGSMIPDLI
                     DQPLTGPNILRIYAATAFALMNWEPRVRISGMSLDITIDGQAHLIIDCIANGSDLQIK
                     VPLKS"
     gene            complement(1312..1956)
                     /locus_tag="VFA14_03260"
     CDS             complement(1312..1956)
                     /locus_tag="VFA14_03260"
                     /inference="COORDINATES: protein motif:HMM:TIGR01644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HZS80434.1"
                     /translation="MNASIAEILRLLNNIVRLGKIEEVKNDRIRVRAGKNLTTWISWI
                     VLRAGATSTWSQPFVGEQCVLLSPNGDMASAVALIGLYSAQHPAPTTESDLHCVKFPD
                     GAIIQYDHASHALRAALPDGGKAELTAPVSVVVHSASITLDAPDTTCTGNLTVQKKLR
                     YLGGMSGKGGEGGEDAAVIEGSLRASDDVIAGNISTRGHHHIEHDGPLTSKAKP"
     gene            complement(2172..>2437)
                     /locus_tag="VFA14_03265"
     CDS             complement(2172..>2437)
                     /locus_tag="VFA14_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006413658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HZS80435.1"
                     /translation="RIKRRSQAMFAKIRTARYMKLETTESQLAIGFFGRIARIAGVHQ
                     RGLRDKVAKNGPDYQYPARPLLGFSATDREMIRDRLLDELRSL"
BASE COUNT          479 a          660 c          736 g          562 t
ORIGIN      
        1 tgccgagatc cgcacaggtg cgcgccacgg cgcgttgcag ggccgcggtt ttgggcggca
       61 aggttgcgac cgcgtcagac atccatcacc ttggtggtta cggtgatgtt cgtgcagtat
      121 gcggcctgtt gactcgtcag aacgatatcg gtggatggtt cgatgagatt taccttgatc
      181 acgccttcgg cgtgcagaac cgcatccaac ttcgagcgat agacgccacg tcccagcggg
      241 cgctttttct ccgtttcgtc ttgcgcgcgc tggattgcgg cgtcggaaat gatgctggac
      301 tcgggaccgg gaggaacata gatctctgcc acgatttcgt agggaatgac ctcggcgctt
      361 tgcacggtga ctagatcacc gatcggccgg atgtcttcgt ggcgtagtgc tgcggtgatg
      421 gcgtcgagca atgcctgatc agccgttccg tcaccttcgg tcgacaagac tgcgatgacg
      481 acttcacatg gtgcgggact ggcggcacga gcgtcggcga cctggccgtg tgcgctgcgg
      541 gcgaaaaatt cgtaagctgc tccgggaccg gcgacagaca gaccgtcgaa cgcctcctga
      601 atgcgcaatt tcagggactc ttcatcttcg cggacttccg cgacaggtgg aacggcatcc
      661 ggatctgccg gcgtgacgat gagttgctcg atgttgaaat tggcaccgat gccgcgtaaa
      721 tctgcgccct tcgcataggc gagcatgacc gctcgcgctg catcgttgat gcggttacgc
      781 agcaccattt cctgcagggc gttttcttcc agcaagatag tcagcggttc ggattccagc
      841 tgcagcgtgg cttcaatttc gttgcggcga tcttcaggaa cgagggtcag cagctttgcc
      901 ttgcggttgg caaggatctg ctcgtagtcc agcgattcga tgatgttcgg agccggcaac
      961 tgcgtcaagt cgatcgggct gctcatgatt tcagcggaac ctttatctgc agatcgctac
     1021 cgttggcgat gcagtcgatg atcagatgcg cctgcccatc gatcgtgatg tcgaggctca
     1081 tgccggaaat acgcacgcgc ggctcccagt tcatcagggc gaaagcggtg gcggcataga
     1141 tccgcaggat gttggggccg gtcaatggtt gatcaatcag atccggaatc atcgaaccat
     1201 aggtgcggcg catgacgcgg ctaccgatcg gcgtattgag aatatccgcc atcgattgac
     1261 ggagatggtc cacgccgctg atcgcgcgac cacttgcggc gttcatgccg atcatggttt
     1321 cgccttcgat gtcagcggac catcatgctc gatatgatga tggcctcgtg tgctgatatt
     1381 gccggcaatg acatcatcgc ttgcgcgtaa acttccttcg atgacggctg catcttcacc
     1441 accttcgccg cccttgccgg acatgccacc gagataacga agtttcttct gcacggttaa
     1501 attaccggtg caggtcgtat ccggagcgtc cagcgtgatg gaggcggaat gcacaacgac
     1561 tgatacgggc gcagtcagtt ctgcttttcc tccgtcgggc aacgcggccc tgagcgcatg
     1621 ggaagcatgg tcatactgga tgattgcgcc gtcagggaac ttgacgcaat gcagatccga
     1681 ttcggttgtc ggggcggggt gttgagcgga ataaaggcca atcagcgcga cggcagaagc
     1741 catatcgcca ttgggcgaca gcagaacaca ttgttcacca acgaacggtt gcgaccacgt
     1801 gcttgtcgcg ccggcacgca atacgatcca actgatccat gtggtgagat tcttgccggc
     1861 gcgaacacgt atacgatcgt ttttgacctc ttcgatcttt ccgagacgga cgatgttgtt
     1921 gagtagccgg agtatctctg cgatgcttgc attcatgcat agcatgatgc gttttcatcg
     1981 ctcgataatc atcatcggcg ggatgtacat gcgacgtgta catcagacgg gaaatttccc
     2041 acgcgcagac cgcgacttcg attcatttta atttcctctg ttttattgaa aagttaaaag
     2101 catgcagcgt accgatatcc gctataccgt cggcggtagc gacagggcag cgcgcactgg
     2161 cgaattgtcg atcacaaact tctaagctcg tccaacagcc tgtcgcggat catttctcga
     2221 tccgtggctg aaaagccgag tagtggccgg gccggatatt gatagtccgg gccattcttc
     2281 gcaaccttgt cgcgcaggcc gcgctgatgc acgccggcga tccgggcgat gcggccgaag
     2341 aagccgatgg ccagttggct ttcggttgtt tcaagcttca tgtagcgggc ggtacggatc
     2401 ttggcgaaca tggcttggct gcggcgtttg atgcggc
//
LOCUS       DASRDH010000144         3172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_719964,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000144 DASRDH010000000
VERSION     DASRDH010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 3172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3172
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_719964"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="VFA14_03270"
     CDS             complement(<1..417)
                     /locus_tag="VFA14_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869989.1"
                     /note="universal genome maintenance protein;
                     Kae1/Qri7/OSGEP/YgjD family protein; in Archaea, some Kae1
                     are found as fusion proteins similar to two distinct
                     proteins in yeast that are involved in the KEOPS complex;
                     kinase associated endopeptidase 1 (Kae1) and a
                     serine/threonine protein kinase (Bud32); in Pyrococcus
                     Kae1 has atypical AP endonuclease activity and inhibits
                     the kinase activity of Bud32; the protein is essential in
                     Escherichia coli and Bacillus subtilis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HZS80436.1"
                     /translation="MIVLGVESSCDETGLALYDTQKGLLAHALHSQIAMHEEYGGVVP
                     ELASRDHIRHAIPLLRQVFEKSGIAHQDIDAIAYTQGPGLAGALLAGASVACGLGLSL
                     DKPVIGIHHLEGHLLSPLLSPNPPPFPFIALLVSGGH"
     gene            580..810
                     /locus_tag="VFA14_03275"
     CDS             580..810
                     /locus_tag="VFA14_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS80437.1"
                     /translation="MTPYRNLHGNSDVLCYEMAEDSIHVVFKSGTYRNYIYDVSHPGK
                     EVVEEMKRLAAQGFGLGRYIASTAKHDFAKKW"
     gene            1130..1885
                     /locus_tag="VFA14_03280"
     CDS             1130..1885
                     /locus_tag="VFA14_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HZS80438.1"
                     /translation="MKVLVPVKRVVDYNVKVRVKTDGSGVDIANVKMSMNPFDEIAME
                     EAMRLKESGRVTEVIAVSCGVTSCQETLRTAMAIGADRGILVEIATDVDLQPLAVAKL
                     LKALADKEQPQLIILGKQAIDDDSNQTGQMLAALLGWPQATFASKVVLEDGKVSVTRE
                     VDGGLETLSLVLPAIVTTDLRLNEPRYVTLPNIMKAKKKMLDIVKPQDLGVDITLRVK
                     TLKVVEPPRRSAGVKVPDVATLVAKLKNEAKVI"
     gene            1898..2833
                     /locus_tag="VFA14_03285"
     CDS             1898..2833
                     /locus_tag="VFA14_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013233521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HZS80439.1"
                     /translation="MSALVIAEHDNASLKGCTLNTITAAIQTQAGGDVHVLVAGHNAG
                     AAATAAAKIAGVSKVLLADAPQLADGLAENVAEQALAIARNYSHLLAPATAYGKNILP
                     RVAARLDVAQISDIIRVDSPDTFERPIYAGNAIATVQSTDGIKVITVRATGFDAAAGG
                     GNATVESITAVADTGKSSFVSREMARSDRPELTAARIVVAGGRGMGSSENFKILEALA
                     DKLNAAMGASRAAVDAGYVPNDWQVGQTGKIVAPALYIAVGISGAIQHLAGMKDSRVI
                     VAINKDEEAPIFSVADYGIVGDLFDIVPELVKQLG"
     gene            2939..>3172
                     /locus_tag="VFA14_03290"
     CDS             2939..>3172
                     /locus_tag="VFA14_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017454120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HZS80440.1"
                     /translation="MGYVAPLKDMLFVLNELAGLPEVSTLPHCEDARPEIVAAILEEN
                     ARFCSEVVAPLNHAADKEPSFWSDGEVTTSKGFK"
BASE COUNT          740 a          852 c          977 g          603 t
ORIGIN      
        1 gtgaccgccg gacaccagca aggcgatgaa gggaaacggc ggcggattgg gcgacagcag
       61 tggcgacaac aaatggcctt ccagatggtg tataccgatg accggcttgt ctagcgacag
      121 ccccaggccg caggcgaccg aggcgcctgc gagcagtgcg ccggccaggc cggggccttg
      181 ggtataggca atggcatcga tgtcctgatg ggcgataccg cttttctcaa atacctggcg
      241 cagcagggga atggcgtgcc gtatatggtc gcgcgaggcc agttcgggga caacgccgcc
      301 gtattcttca tgcatggcga tttgcgaatg cagggcatga gcgagtagac ctttttgcgt
      361 atcgtataac gccaggccag tttcatcgca ggaagattcg acgccgagaa cgatcatgga
      421 gtgcagcagg ggagcgcatg gaaaactgca gcaaggcctg gccccggcgg gacaagtgga
      481 aaaactgcat gataaacttg attggcgggt tgcaaagcag cttgcagcaa cgcaacaggt
      541 caaaaattct accacattta ccgttgaatg ggagttgaaa tgacacctta tcgcaacctc
      601 catggaaact cggatgtcct ttgttacgag atggcggaag actccatcca tgttgtcttc
      661 aagagtggaa cgtatcgcaa ctatatctac gacgtgtctc atccaggaaa ggaggtggtc
      721 gaggaaatga agcggcttgc ggcgcaaggt tttggtttgg gcaggtatat tgcctcgact
      781 gcgaagcacg attttgcaaa gaaatggtga ttcagccgct gatacatagc atcagaaaga
      841 agatacatgc gcgtgagtgc aactggtcgt cgtcactcat tggcgacgac cggcatgaaa
      901 ttccacacag cgatccagct caaggctggg gcggcagatc cggccatcga gtattaccgt
      961 tagggggcgg cagccagtca tttttcgcag gccgatttcg atgaagcgta tttccgcaac
     1021 gcttcggaag aatacgtcgg ttgccgcata gtggcggcga tcttctgtat ttgcttttat
     1081 aattctcccg ttcagttgac gtcgattcga tttttccaag gaccaggcga tgaaagtatt
     1141 agtccccgtc aagcgggtag tggattacaa cgtcaaggtg cgcgtcaaaa ccgatggcag
     1201 cggggtggac attgctaacg tcaagatgtc gatgaatccc tttgacgaaa tcgccatgga
     1261 agaagccatg cgcctgaaag agagtggcag ggtcactgaa gtgatcgcgg tgtcctgcgg
     1321 cgtgacgtca tgccaggaaa cgctgcgcac cgccatggcc atcggcgccg accgtggcat
     1381 tctggtcgaa attgccaccg atgtggatct gcagccgctg gcggttgcta agctgctcaa
     1441 ggcgctggct gacaaagagc agccgcaatt gatcatcctt ggcaagcaag ccatcgacga
     1501 cgacagcaat cagaccggcc agatgctggc ggcgctgctg gggtggccgc aagcgacgtt
     1561 tgcttcgaaa gtcgtgctgg aagacggcaa ggtgtcagtg acccgcgaag tcgatggcgg
     1621 actggaaacc ctgtcgctgg ttttgccggc catcgtgacc accgacctgc gcctgaacga
     1681 accgcgctac gtcaccttgc cgaacatcat gaaagccaaa aagaagatgc tggatatcgt
     1741 caaaccgcag gatctgggtg tagatatcac gctgcgcgtg aagaccctga aagtcgtcga
     1801 accgcctagg cgcagcgccg gcgtgaaggt gccggacgtc gccacgctgg tagcgaagct
     1861 gaagaacgaa gccaaagtca tctaaggaag ggacgatatg agcgcgctcg tcatcgcgga
     1921 acatgacaat gccagtctca aaggctgcac actgaacacc atcaccgcgg ccatacagac
     1981 ccaggctggc ggtgatgttc acgtcctggt tgccggccac aacgccgggg cggcagcgac
     2041 tgccgccgcg aaaatcgccg gcgtgtcgaa agtcctgctg gccgacgcgc cgcaattggc
     2101 tgatggtctt gccgagaacg tggccgagca ggcgctggcc attgccagga attacagtca
     2161 cctcctcgcg ccggccacag cctatggcaa aaatatcctg ccgcgcgtcg ccgccagact
     2221 ggacgtggca cagatttccg acatcatcag agtcgacagc ccggacacct tcgaacgtcc
     2281 gatctatgcc ggcaatgcca tcgccaccgt gcagtcgact gacggcatca aggtcattac
     2341 tgtgcgcgcc actggtttcg acgccgccgc cggcggcggc aatgccacag tggaaagcat
     2401 cacggccgtg gccgataccg gcaagtccag cttcgtgtcg cgcgaaatgg ctagatccga
     2461 ccgccccgaa ttgaccgccg ccaggatcgt cgttgccggc ggtcgtggca tgggatcatc
     2521 agaaaacttc aagatactgg aagcgctggc cgacaaactc aacgccgcca tgggcgcttc
     2581 gcgcgcggcg gtggatgcgg gctatgtgcc gaatgactgg caggtcggcc agaccggcaa
     2641 gatcgtcgcg cctgcgttgt atatcgctgt cggcatttcc ggcgcgatcc agcatctggc
     2701 cggtatgaag gattccaggg tgatcgtggc gatcaacaag gatgaagaag caccgatctt
     2761 ttcggtcgcg gactatggca tcgtcggcga cctgttcgac atcgtgcctg aactggtcaa
     2821 gcaactgggt tgagtatcag aaagccaacg gccctgtcaa gagaccgggc cgtatcgatc
     2881 tggaagcgct gaacgcgacg gaagattgcg tgcgtcgatg cgaaatcagg agctggaaat
     2941 gggctacgtc gcacctttga aagatatgct gttcgtgttg aacgaactgg caggattgcc
     3001 agaggtgagt actttgccgc attgcgaaga cgcaaggcca gaaatcgtgg cagccattct
     3061 cgaagaaaac gccagatttt gcagcgaagt ggtggcgccg ctcaatcacg ccgccgacaa
     3121 ggaaccgagt ttctggagcg atggcgaggt cacgaccagc aagggtttca aa
//
LOCUS       DASRDH010000145         2475 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_432875,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000145 DASRDH010000000
VERSION     DASRDH010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432875"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="VFA14_03295"
     CDS             <1..341
                     /locus_tag="VFA14_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012406734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZS80441.1"
                     /translation="SEAINITGYKKYKEALHEDHMPIEESRGFSPLQREVNAFKMALS
                     TLIPVDDKVFKQRFDGNSFFDNPIINCTLEKLVNKALVTVSGNRVTLTPIGITLVEAI
                     INTQFSAESA"
     gene            353..1159
                     /locus_tag="VFA14_03300"
     CDS             353..1159
                     /locus_tag="VFA14_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS80442.1"
                     /translation="MTSTPSASKPVFSLYYIGIDTEAGVTNEQFEAFVCERGMKIPCY
                     PGWRWTLLRGLRGERVGQYLMLYEIDSTEQRDRYVTAAGQKTEEAHAFWRQHADALEA
                     LAEWRKLGTFSQLPTLFTDYRLLAENVKSNVPRGPRYQERPGKEPVARIIGIHNLALR
                     EGVTPAQFEQFIIENHHRIEDYPDWKFHLLKGERGNRLDQYVVMMEIASMAALDVFYP
                     EPDIATAKAAKFAKAHRDTKQMYEEWKQLASFSGSPQIYTDYLSVAESRS"
     gene            1159..2322
                     /locus_tag="VFA14_03305"
     CDS             1159..2322
                     /locus_tag="VFA14_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZS80443.1"
                     /translation="MTLNLQSRSAASNIRQQTATRTIFFLAGLGMSAWAPLIPFVKSR
                     MGIDEGSLGLLLFCIGAGAMSIMPFTGHLIGKLGCRKILLSAGLLLCVDLPLLTLIDV
                     PLFMGAALLAFGAINGIIDVTMNSQAIIVERESGQAKMSGFHGFYSLGNISGAGSVSL
                     LLWTGVTPSFAIGLIAALIALLLLIAAEDLLASSEIDSERKGSALFALSHGTILFIAL
                     LCFFVFLTEGAMLDWSSLFLNAERGVAKSQAGLGFTLYAIAVTLSRLYGDRLINAIGR
                     YRMLLLGSLCAAAGLLLAISINLAWVAFGGLILVGLGIANIVPILFNAVGNQKQVPPN
                     LAFPAVTLVGYIGLLAGPALIGLIAKYVTLTLAFGCTLFCLLLVSISARAVTR"
BASE COUNT          537 a          705 c          674 g          559 t
ORIGIN      
        1 actcagaagc catcaatatc actggctaca aaaaatataa agaagccctg catgaagatc
       61 acatgcctat cgaagaaagc cgcggttttt caccgctcca gcgcgaagtg aatgccttca
      121 aaatggcgct atccaccctg attcccgtgg acgacaaggt gttcaaacag cgttttgacg
      181 gcaacagctt ctttgataac ccgattatca attgcacgct agaaaaactg gtgaacaaag
      241 cattggtaac ggtctctggc aatagggtca cgctgacgcc catcggtatc acgctggtag
      301 aagccatcat taacacccaa ttctcagccg agtctgcata aggagtcgac cgatgacaag
      361 cacaccttct gcatcaaaac cggtattcag cctttattac atcggcattg ataccgaagc
      421 gggcgtgacg aacgaacagt tcgaagcttt cgtctgcgag agaggaatga aaatcccctg
      481 ctatcctggt tggcgctgga ccttgctacg tggcctgcgc ggcgaacgcg tcgggcaata
      541 tctgatgctg tatgagattg acagcacaga gcagcgcgat cgttacgtga ccgccgccgg
      601 ccagaaaacc gaagaggcac acgccttctg gcggcagcat gccgatgcgc tggaggccct
      661 ggccgagtgg cgaaaacttg gcaccttcag ccagttaccg acgttgttca ccgattaccg
      721 tctcctggcc gagaacgtca aaagcaacgt gcctcgcggg ccccgctatc aggaacgccc
      781 tggcaaggag cctgttgcac gcatcatcgg catccacaat ctggcgctgc gcgaaggggt
      841 gacgccggcg cagttcgagc agtttattat tgaaaaccat caccgcattg aggattatcc
      901 ggactggaaa tttcatttac tgaaaggcga acgcggcaac cgactggatc agtacgtggt
      961 gatgatggag atagccagca tggccgcgct ggacgtcttc tacccggagc cggatatcgc
     1021 caccgccaaa gcggcgaaat tcgccaaggc tcaccgcgac accaagcaga tgtatgaaga
     1081 gtggaaacag ttggcgtcgt tctccggctc gccgcagatt tatactgatt acttgtctgt
     1141 cgcggagagc cggtcgtgat gacactgaat cttcagagca gaagcgccgc gagcaatatc
     1201 cgccagcaaa ccgccacccg cacgattttc tttctcgccg ggttgggcat gtcggcctgg
     1261 gcgccattga ttccgttcgt gaagtcgcgt atggggatcg atgaaggctc tctcggttta
     1321 ctgctgtttt gtatcggcgc cggcgcaatg tcgatcatgc cctttaccgg tcatctgatc
     1381 ggtaagctgg gctgtcgtaa aatccttctg agcgccggcc tgctattatg cgtcgactta
     1441 ccgctgctca cgttaataga cgtcccgctg ttcatggggg cagcattatt ggcgtttggt
     1501 gccatcaacg gcatcataga tgtgacgatg aactcacagg ccattattgt cgagcgcgaa
     1561 agcgggcaag caaaaatgtc cggctttcac ggcttctaca gtcttggcaa catttcagga
     1621 gcgggcagcg tcagtttgct gctgtggacg ggggtcaccc catccttcgc catcggcctg
     1681 attgcggcgc tcatcgcact gcttttgctc atcgctgcgg aggatctgct ggccagcagt
     1741 gaaatcgaca gcgagcgcaa gggcagcgcg ctgttcgcgt tgtcccacgg cacaatcctg
     1801 tttatcgccc tactctgctt ctttgttttc ctgaccgagg gtgcgatgct cgactggtca
     1861 tcgctattcc tcaatgccga gcgcggcgtt gccaaatccc aggcaggatt gggttttacg
     1921 ctgtacgcca tcgcggtgac cctcagccgg ctgtatggcg accggttgat caacgccata
     1981 ggacgctatc gcatgctgct gctcggcagc ctgtgcgccg ctgccggttt gctgctggct
     2041 atctcgatca acctggcgtg ggttgccttt ggcggactga ttttggtcgg cctgggcata
     2101 gccaacatcg tgccaatcct gttcaacgca gtgggcaatc aaaagcaggt tccgccgaat
     2161 ctggccttcc cggccgtgac gctggtcggc tatatcggcc tgctggccgg cccggcgttg
     2221 atcggcttga tcgcaaaata cgtcacactc acgctggcct ttggctgcac tcttttctgc
     2281 ctgctgctgg tcagcatcag cgcccgagcg gtgacgcgct aatccagccc acttacatca
     2341 cgaggacatc ttcatgcatg catcatcaag cccggtcttc tccctacact acgtgggtat
     2401 taacctgaaa agagggatcg cctccgatca attcgaaaat ttcgtatgcc agaaaggcgt
     2461 caatattacg gccta
//
LOCUS       DASRDH010000146         2762 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_195660,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000146 DASRDH010000000
VERSION     DASRDH010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2762
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195660"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="VFA14_03310"
     CDS             complement(<1..196)
                     /locus_tag="VFA14_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013232735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HZS80444.1"
                     /translation="MSMNLVTLLYLVASICFIQALKGLSHPSTARRGNAFGIAGMVIA
                     VLTTLALIIKLKGGVPDSGIG"
     gene            complement(193..510)
                     /locus_tag="VFA14_03315"
     CDS             complement(193..510)
                     /locus_tag="VFA14_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HZS80445.1"
                     /translation="MEVGHTITNLIIFVLAIYVGYHVVWTVTPALHTPLMAVTNAISA
                     IIIIGAMLAAGLTEGLTGRVAGTLAVALAAVNVFGGFMVTRRMLEMFRKKEAKTRADK
                     AGT"
     gene            complement(539..1654)
                     /locus_tag="VFA14_03320"
     CDS             complement(539..1654)
                     /locus_tag="VFA14_03320"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008325649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HZS80446.1"
                     /translation="MKLGIPVESREGETRVAATPETVRKLIAAQHEVLLESGAGISSS
                     LPDEAYAAAGARIAAVGDVYDAEVILKVRAPSQQERARLKSGTVVIGMLDPYDVDNIA
                     VMAAAGLTAFALEAAPRTTRAQSMDVLSSQANIAGYKAVLMAANTYQRFMPMLMTAAG
                     TVKAARMLIMGAGVAGLQAIATARRLGASIEASDVRPAVKEQVESLGAKFLDVPFITD
                     EEKEIAQGVGGYARPMPDDWMRRQAALVHERARQADIIITTALIPGRKAPVLIGEETV
                     KAMKPGSVILDMAVDQGGNCPLSESGKTVIRHGVHIIGAGNLAALVAADASALYARNV
                     LDFLKLIVDAEGKLAIDRADDIVAATLLCAGGEVLRK"
     gene            1868..>2762
                     /gene="mnmA"
                     /locus_tag="VFA14_03325"
     CDS             1868..>2762
                     /gene="mnmA"
                     /locus_tag="VFA14_03325"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008325643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HZS80447.1"
                     /translation="MAKKKVVIGMSGGVDSSVSAWLLKEQGYEVIGLFMKNWEDDDDD
                     AYCSTRQDWIDAACVADVIGVDIEAVNFAAEYKDRVFAEFLREYQAGRTPNPDVLCNA
                     EIKFKAFLDHAMLLGADLIATGHYARVRKHPADKCELMKAADASKDQSYFLHRLNQQQ
                     LSKTLFPLGEMYKTEVRRIARQLKLPNATKKDSTGICFIGERPFRAFLNRYLAYRPGP
                     MKTPEGDIVGEHVGLSFYTLGQRKGIGLGGVKAHQKADGNSDAWYVARKDIENNTLYI
                     VQGHDHPWLLSSQLGAGQISWV"
BASE COUNT          586 a          832 c          798 g          546 t
ORIGIN      
        1 agccgatgcc ggaatccggc acgccaccct tgagcttgat gatgagcgcg agggtcgtca
       61 ataccgcaat gaccatgccg gcgatgccga aggcattgcc gcgacgcgcc gtcgagggat
      121 gtgacaaacc tttgagcgcc tgaatgaagc agatcgaggc gaccagatac agcagggtga
      181 ccagattcat gctcatgttc ccgccttgtc cgccctggtt ttggcttctt ttttccggaa
      241 catctccagc atgcgccgcg tgaccatgaa gccgccgaaa acattgaccg ctgccagcgc
      301 cacggccagt gtgccggcga cgcggccagt cagtccttca gtcaggccgg cggccagcat
      361 ggcgccgatg atgatgatcg ccgagatcgc attggtgacc gccatgagcg gtgtgtgcaa
      421 tgcgggcgtc acggtccaga ccacgtggta gccgacgtag atcgccagca cgaagatgat
      481 caggttggtg atggtgtgac cgacttccat ataattttct ctgtcttgcc gaatgttctt
      541 atttacgcag cacttcgccg cccgcgcaca acagagtcgc ggcgacgatg tcgtctgcac
      601 gatcgatcgc cagcttgcct tcggcatcga cgatgagctt gagaaaatcg agtacgttgc
      661 gcgcgtacaa tgccgaggca tccgccgcta ccagcgccgc cagattgcct gcgccaatga
      721 tgtggacgcc atgcctgatc acggtcttgc cggattcgga cagcggacag ttgccaccct
      781 gatcgactgc catgtcgaga atgaccgagc cgggcttcat ggccttgacg gtctcttcac
      841 caatcagtac cggcgccttg cggcctggaa tcagggcagt cgtgatgatg atgtcggcct
      901 gcctggcgcg ctcatgcacc agtgcagcct gacgccgcat ccagtcgtcc ggcatcggac
      961 gggcatagcc accaacgcct tgagcgattt ctttttcttc gtcggtgatg aaaggcacat
     1021 caaggaattt ggcgcccaac gactcaacct gctccttgac cgcagggcgc acgtcggagg
     1081 cctcgatcga cgcgcctaga cgcctggcgg tggcaatcgc ctgcaaaccg gccacgccgg
     1141 cgcccatgat cagcatgcgc gcagccttga cggtgccggc ggcagtcatc agcatgggca
     1201 tgaagcgctg gtaggtattc gcggccatga gcacggcctt gtagccagcg atattggcct
     1261 gcgacgacag cacatccatc gattgcgcgc gcgtggtccg cggcgcggct tccagcgcaa
     1321 aagcagtgag tccggcggcg gccatgacag cgatattgtc aacatcgtag ggatcgagca
     1381 tgccgatcac gaccgtgcca gacttcagac gggcgcgctc ctgctgactg ggtgcgcgca
     1441 ccttcaaaat cacttcggca tcatagacat cgccgacggc ggcgattctc gcgccggcag
     1501 cagcataggc ttcgtcagga aggctggagg aaatgccggc tcccgactcg agcaatactt
     1561 catgttgcgc cgcgatcagt tttctgaccg tttccggagt ggccgctacg cgcgtttctc
     1621 cttcccgact ttcgacaggg atgcctagct tcattcaaaa ttcctctggc tgttatcaat
     1681 tccggtcgaa atcttacacg taaaaatcaa cagtcgatca ttttacatcg gcttccgtca
     1741 ttgcatgatg ccgctcgcct gtctggctaa gcgcctgaag catctgcccg gccaaggtaa
     1801 aatgtcgcct cttgcaagga gagtacgttt atgactgaag tgactgcgat gccgggagaa
     1861 gcatcccgtg gctaagaaaa aggtcgtcat cggcatgtcg ggcggcgtcg actcgtcggt
     1921 ctccgcctgg ttgctcaagg aacaaggcta tgaagtcatc ggtctgttca tgaagaactg
     1981 ggaagacgat gacgatgacg cctattgctc gactcgccag gactggatcg atgcggcctg
     2041 cgtggccgat gtgatcggcg tggacatcga ggccgtcaat ttcgccgccg aatacaaaga
     2101 ccgcgtgttc gccgaattcc tgcgtgaata ccaggccgga cgcacgccca atcccgacgt
     2161 actctgcaat gccgagatca agttcaaagc cttcctcgac catgccatgc tactgggcgc
     2221 cgatctgatc gccaccggtc actatgcgcg tgtgcgcaag catcccgccg acaaatgcga
     2281 actgatgaaa gccgccgatg ctagtaaaga tcagagttac ttcctgcaca ggctgaatca
     2341 gcaacagcta tcgaaaacct tgttcccgct cggcgagatg tacaaaaccg aagtacgcag
     2401 gatcgccagg cagttgaaac tgccgaacgc gaccaagaaa gattcgaccg gcatctgctt
     2461 catcggcgaa cggccatttc gcgccttcct caaccgttac ctggcctatc ggcccggccc
     2521 gatgaaaacg cccgaaggcg atatcgtcgg cgagcacgtc ggcctgagtt tttacacact
     2581 gggccagcgc aaaggcatcg gcctgggcgg cgtcaaggcg caccagaagg ccgacggtaa
     2641 cagcgatgcc tggtatgtcg cgcgcaaaga catcgagaac aatacactgt atatcgtaca
     2701 aggccacgac catccatggc tgctgtcatc gcaactgggc gctggccaga tcagctgggt
     2761 gg
//
LOCUS       DASRDH010000147         8841 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_827141,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000147 DASRDH010000000
VERSION     DASRDH010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 8841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8841
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_827141"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            147..881
                     /gene="rlmB"
                     /locus_tag="VFA14_03330"
     CDS             147..881
                     /gene="rlmB"
                     /locus_tag="VFA14_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HZS80448.1"
                     /translation="MKNKMIFGFHAVTSRIRLEASSVEELYVDAQRDDARMRDLLHAA
                     RTCGVRIIQANDQRLSNMVGTHRHQGVVARAGGLSLAHNLDELLDTVEGPPLLLILDG
                     ITDPHNLGACLRVADGAGVHAVIAPRDRAVGLNATAAKVASGAAETVPYITVTNLARA
                     MRDLKERGIWLIGASDAADRNVYDANFSGPTALVMGSEGEGMRRLTRETCDILVAIPM
                     SGLVESLNVSVAAGVCLYEAKRQRLS"
     gene            990..1739
                     /gene="cysE"
                     /locus_tag="VFA14_03335"
     CDS             990..1739
                     /gene="cysE"
                     /locus_tag="VFA14_03335"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HZS80449.1"
                     /translation="MFTQLREDISSIIERDPAARNTWEVLTCYPGLHALMLHRIAHAC
                     WKSGLKWLGRLISQLARWLTGIEIHPGAIIGRRVFIDHGFGVVIGETAEINDDCTIYQ
                     GVTLGGTSLDRSAKRHPTLGRGVIIGAGAKVLGSFCIGDGAKVGSNAVVIREVPPGAT
                     AVGNPAHVIRKETPDTCEQAAARMFAAYGGVTPNDDPLSKALHSLIDQAAAQEHQIEA
                     ILAAIRSAGIGCEKLQGLEKFDAAQLNRLVE"
     gene            complement(1757..2533)
                     /locus_tag="VFA14_03340"
     CDS             complement(1757..2533)
                     /locus_tag="VFA14_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007881180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HZS80450.1"
                     /translation="MPKIISANLNGIRSAARKGFFEWMHKESADFICVQELKAQEIDM
                     TPELLAPQGYVGHFHYAAKKGYSGVGLYSKRMPNAVCIGFGVEEFDQEGRYIECDFGD
                     LTVISLYCPSGSSSEERQQAKFRFMDVFLPHLQALKNSNREIVICGDWNIAHKEIDLK
                     NWKNNQKNSGFLPEERAWLTHVFDELGLVDVYRVLHPNATGDAYTWWSNRGQAWANNV
                     GWRIDYHIATAGIAGKSRTAAIYKERRFSDHAPLTIDYKT"
     gene            2661..3323
                     /gene="pyrE"
                     /locus_tag="VFA14_03345"
     CDS             2661..3323
                     /gene="pyrE"
                     /locus_tag="VFA14_03345"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006464581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HZS80451.1"
                     /translation="MSSLRQEFIEFSVSTGVLRFGEFVTKAGRISPYFFNAGLFNEGA
                     SLAKVAQFYARTLSASGLEYDMLFGPAYKGITLASATAVALADLSRNVPFAYNRKEAK
                     DHGEGGMTVGAKLQGRVVIVDDVISAGTSVHESVDMIRAAGATPCAVLVALDRMERSG
                     KDDALSTYSAAQEVTKTYGMPVISIGNLNDLLTYLAHANADAQLSQYKDAVAAYRTRY
                     GV"
     gene            complement(3475..3828)
                     /locus_tag="VFA14_03350"
     CDS             complement(3475..3828)
                     /locus_tag="VFA14_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS80452.1"
                     /translation="MQTSTMKRSGFSSVRYDSAAVPMKNTPMHLVVARKALPVPACTL
                     KRDLSLQSHFMMLHLCWSEQRFNEQKWDKKRKRQERREDSITPLKQAIWPMMVIWQGG
                     APRGSCATASEAATA"
     gene            4489..5148
                     /locus_tag="VFA14_03355"
     CDS             4489..5148
                     /locus_tag="VFA14_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004628787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZS80453.1"
                     /translation="MTWAGAKKIRIALLDDHAVVRHGLVACLREEEDFEVVGAYANSR
                     ELISALGSLFVDILLIDYSLSADDNDGLNLIRALKVRFPGSNIIVSSAHYNPATVALT
                     MRAGALGFFGKEQALPELIAAVRSVSVGRMHLDPLMEAEISAMLSSDQSNSNSLINST
                     ELSPREREVLRCCLEGMSVTQIAEKFMRSVKTISGQKRTAFRKLGVHNDNEFFKIQHQ
                     C"
     gene            5208..5915
                     /locus_tag="VFA14_03360"
     CDS             5208..5915
                     /locus_tag="VFA14_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007880080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZS80454.1"
                     /translation="MTTTMSAHAPLVLVVDDSATSRVVIEKQLKKLGCNVTVARDGTS
                     GLAAIVQTEFDLVLLDCQMPDMSGYDVAKLAREQKNEGPYTPIIAISAETDMEHKQRC
                     LESGMDGVLGKSFPVAGELKKILSLWCNFDLVAASSTEIDAMENIELQTLFRTISLED
                     LNTMQTLVQSDIPGVGRMAHRMKGAALTMGAAEIVATLERIEAIIAANGTSLASSLRT
                     EIGILRQQCDVLEKCAD"
     gene            6088..6576
                     /locus_tag="VFA14_03365"
     CDS             6088..6576
                     /locus_tag="VFA14_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS80455.1"
                     /translation="MQGRIGDEWKREIFVTKTPDLQDSVAELSLQGTRRNIQRWLDQS
                     GMYPPEGAPEEYSFYALECYPTAQDRSAFFHQFIACSKPHIGYQLLALLGGTGTVRSV
                     WTTNFDGLTARALGAANVVCVEVGIDTPQRTVRAQGNNEVRLLSLHGGFRYGFLQPNG
                     TA"
     gene            complement(7102..7758)
                     /gene="adk"
                     /locus_tag="VFA14_03370"
     CDS             complement(7102..7758)
                     /gene="adk"
                     /locus_tag="VFA14_03370"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HZS80456.1"
                     /translation="MRLILLGAPGAGKGTQATFIREKYNIPQISTGDMLRAAIAAGTE
                     LGIAVKRVMDAGRLVSDDIIISLVEGRLQQADCVNGYLFDGFPRTVPQADAMRKAGVQ
                     IDYVLEIDVPDSAVVERMSGRRVHPASGRIYHVKFNPPKLDGKDDVTGEALVQRDDDK
                     EETVRRRLSVYHEQTEVLVGYYSKWAESVQPGAPKYLKITGVGPVEQIRDSVFAALSS
                     "
     gene            complement(7913..8714)
                     /gene="kdsB"
                     /locus_tag="VFA14_03375"
                     /pseudo
     CDS             complement(7913..8714)
                     /gene="kdsB"
                     /locus_tag="VFA14_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463743.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
BASE COUNT         2107 a         2295 c         2379 g         2060 t
ORIGIN      
        1 ggtaagcgcc cggctaaagg gcctgctccg gtccggccgg aaaaagcgtc gaagcagcag
       61 cggcgcatgc gtcggtcagc gtgactggac gccaatcggg cgctgaaaca ttcggcgctg
      121 attcatcgaa ggaagaaaca aggctcatga aaaataaaat gattttcggc ttccacgctg
      181 tcacctcccg tattcgtctc gaggcgtcgt cagtggagga attatatgtc gatgctcagc
      241 gtgatgacgc ccgtatgcgg gatttgctgc atgccgctag gacttgcggc gtgcgtatca
      301 tccaagcaaa cgaccagcgc ttgtcgaaca tggtcggcac gcaccgccat caaggcgtag
      361 tcgccagggc cggcggactg tcgctggcgc acaatctgga cgaacttctg gataccgttg
      421 aaggtccgcc attgttgcta atactcgacg gcattactga tccgcacaac ctcggcgctt
      481 gcctgcgtgt ggccgatggc gccggcgtac atgccgtgat tgcgcctagg gatcgcgccg
      541 tcggcttgaa tgccacggct gccaaagttg ccagtggcgc tgccgaaacc gtaccctata
      601 tcaccgtcac gaatctggcg cgcgccatgc gcgacctcaa ggagcgtggc atctggctga
      661 tcggtgccag tgatgccgcc gaccggaacg tatatgacgc caatttctcc ggccccacgg
      721 cgctggtcat gggttcggag ggagaaggca tgcgtcgctt gacacgcgaa acctgcgata
      781 ttctggtcgc tattcccatg tccggtttag ttgaaagcct gaatgtctcc gttgccgccg
      841 gcgtctgtct gtatgaagcc aaacggcagc gtctgtcata acactagacg acgccggtaa
      901 ccgctcccgc gaagcggtgg ccttgtcatg tttttctccc tgatctccgg aaaaaccgtt
      961 cttgcatacc ctgcatcgat gacaacatta tgtttaccca acttcgagaa gatatctcca
     1021 gcattatcga gcgcgatccg gcggcacgta acacctggga agtactgacc tgttatccgg
     1081 gcctgcatgc gttaatgctg catcgcatcg cacacgcttg ctggaagagt ggcttgaagt
     1141 ggctggggcg tttgatctca caattggcgc gctggctgac cgggatcgag atccatcccg
     1201 gtgcaatcat cggtcgtcgt gttttcatcg atcatggctt cggtgtggtg ataggcgaaa
     1261 ccgcggaaat caacgacgac tgcacaattt atcaaggcgt gaccctgggc ggcacttcgc
     1321 tcgacaggag cgccaagcgt catcccacgc tggggcgcgg cgtcatcatc ggtgccggtg
     1381 cgaaagtact gggcagcttt tgcatcggcg acggtgccaa ggtcggctct aatgcagttg
     1441 tcatcaggga agtgccacca ggcgcaacgg cagtcggtaa tccggcgcac gtcatacgca
     1501 aggaaacgcc ggacacatgc gagcaagccg ccgcgcgcat gtttgccgct tacggcggcg
     1561 taacgcccaa cgacgatcct ttatcgaaag ccctacacag cctgatcgat caggctgctg
     1621 cgcaagaaca tcagatcgaa gctatcctgg ctgcgatcag gtcagccggt attggttgcg
     1681 aaaaattgca ggggttggag aagttcgacg ctgcgcaact gaacaggctg gtggagtagg
     1741 gaagagcaga tagccgtcac gtcttgtaat cgatcgtcag cggcgcatga tcggagaagc
     1801 gtcgctcctt gtaaatcgca gcagtcctgg actttccggc gatgccggcg gtggcgatgt
     1861 gatagtcgat gcgccatccg acgttgttag cccaggcttg gccgcgattg ctccaccagg
     1921 tataggcatc gccagtagcg ttcggatgca gcacacggta gacgtcgacc aagccgagtt
     1981 catcaaatac atgtgtcagc caagcgcgtt cttccggcag gaagcctgaa tttttctggt
     2041 tgtttttcca gttcttcagg tcgatttcct tgtgagcgat gttccagtcg ccgcaaatga
     2101 cgatttcgcg gttactgttt ttcaacgcct gcaagtgagg taaaaacacg tccataaagc
     2161 ggaacttggc ctgctggcgt tcttcacttg atgaaccaga agggcaatac agcgagatca
     2221 ccgtaaggtc gccgaagtca cattcgatat agcggccttc ctggtcgaat tcttctaccc
     2281 cgaagccgat gcacacggcg tttggcatgc gcttgctgta caggcccaca cccgaatagc
     2341 ctttcttcgc agcgtagtgg aaatggccaa cgtaaccctg cggtgcgaga agctccggcg
     2401 tcatgtcaat ttcctgtgct ttcaattctt gtacgcagat gaagtcggca gattccttgt
     2461 gcatccactc gaagaagccc tttctggcag cagagcgaat gccattaaga ttggcggaaa
     2521 taattttcgg catgaaacaa cccacatgaa agtcaggcgc gtaggatacc tgattcattt
     2581 agaatgaggg ccttacagat ttttgccatt ttttgctgtt atgcagaaat tttctgcata
     2641 ccgtgcttcg ggaagatatt ttgagcagtt tacgacaaga atttatcgaa ttttccgtgt
     2701 ccacaggcgt gctgcgcttc ggcgagttcg tcaccaaggc tggtcgcata tcgccttatt
     2761 ttttcaatgc cggcctgttc aacgaaggcg catccctggc caaggtagcg cagttttatg
     2821 cgcggacact atcggcatca ggtctggaat acgacatgct gttcggcccg gcctataaag
     2881 gtattacgct ggcatccgcg actgctgtag cactggcaga cctgagccgc aatgtaccgt
     2941 tcgcctacaa ccgtaaagaa gccaaggacc atggcgaagg cggcatgaca gtcggcgcca
     3001 agctgcaagg ccgtgtagtg atcgtggacg atgtgatttc ggcgggtacc tcggtgcatg
     3061 aatcggtcga catgatccgc gctgccggtg cgacgccatg cgcggtgttg gttgctctcg
     3121 accgcatgga gcgttcgggt aaggacgacg ccttgtcaac gtattcggca gcgcaggaag
     3181 tcaccaaaac ctacggcatg ccggtaattt cgatcggcaa tctgaacgac ctgctgacat
     3241 atctggcgca tgccaacgct gatgcgcaac tgtcacaata caaggatgcc gtggcggcat
     3301 accgcacgcg gtatggcgtc tgaaaatctc aggcgtaggc gtatcccggt gaaaacaacc
     3361 cctcattccg cttgaacgtg gccgttgtgc agcgagcggt atagcgcggc tgaatttatc
     3421 taatgtcgtc ggcccggcga gatggtggcg atacccccat gcaccgctcc acgactacgc
     3481 agttgctgct tcggatgcgg tagcgcagct tcctcgcggc gcgccacctt gccatatcac
     3541 catcatcggc cagatagcct gtttgagcgg ggtaatactg tcttcacgtc gttcctgtcg
     3601 tttccgtttt ttgtcccatt tctgttcgtt gaaccgctgc tcgctccagc acagatggag
     3661 catcataaaa tggctttgca agctgaggtc gcgtttcagt gtgcatgctg gtaccggcaa
     3721 tgctttacga gcgacgacaa gatgcatggg cgtatttttc atcggcaccg cggcactgtc
     3781 gtagcgcact gatgaaaagc cggatcgttt catagtggat gtctgcatgt tttttttctg
     3841 tgcattgaca ccgcgctcgt cagtcccctc ataaactcct gacagcagta ccacaagcag
     3901 cactaccaac aatgtgtaaa ttcttgagca tggcatgagt ttccccttca gttttcctta
     3961 tcaacatagc tttgatatag ctttgatcca gaaagagcca actaaccctt catgactttt
     4021 tgaaaagaga ttgtccgagt tacagtgtga cattcatata agaaaattct gaaaatgcat
     4081 ttttcgttaa gaaaagcgaa aaacagtggg aagcaaatct gataaatcaa atacaaaaag
     4141 actaaatacg cgctgtcaaa aaaccatatg aaatagatag ggatgatgga aaagtctgtt
     4201 ttctttcatt tcaagaaaac tctcattttt aagaaaattc tcattttcgt acctgaacag
     4261 ggagagcata atagcctcgc tatgttgtga tcattgctga tttagtcatt ggtaacgata
     4321 ggagggtagt gtgtatacgg aagcagatga acagggaaat attttttgta gaaactggtt
     4381 cagtagtgac atcgtcggtg tgtaaattga ccatgatttt ttcgtgatag catcaaaata
     4441 atattcattg gttttgccgt gcagatggaa atttgtgaaa gaatcatgat gacatgggct
     4501 ggagcaaaaa agattcggat tgccttgctg gatgatcacg cggttgtgcg ccatggactc
     4561 gttgcgtgct taagggagga ggaggatttc gaggtagtag gcgcttatgc caacagcaga
     4621 gaattgatat cggcattggg ttccttgttt gtggacatat tgctgatcga ttactcattg
     4681 agcgcagacg ataacgatgg gctgaatctg attcgcgcgt tgaaggtgcg tttccccggc
     4741 agcaatatca tcgtttcctc tgcacattac aatccggcca cggtcgcgtt gacgatgcgc
     4801 gcgggggcac tcggattttt cggcaaagag caggcactgc cggaactgat tgctgcggtt
     4861 cgttcggtat cggtcggacg gatgcatctc gatccgctga tggaggcaga aatttcggcg
     4921 atgctatcta gtgatcagtc aaacagcaat tcgctgatca atagtacaga attatcaccg
     4981 cgtgaaagag aagtgctgcg ctgctgcctg gaaggcatga gcgtgaccca gatcgccgag
     5041 aaattcatgc gcagtgttaa gacaatcagc ggacagaagc gaacggcctt tcgcaaactg
     5101 ggcgtgcata acgataacga gtttttcaag attcagcatc agtgctaggc gctgacaagg
     5161 ctgataccgt cacatcacca atgagttctg gaatttaaag gcttgtcatg acaactacta
     5221 tgtcggcgca tgcgccattg gttctcgtcg tcgatgattc cgcaactagt cgcgtcgtca
     5281 tcgaaaagca gttgaaaaaa cttggctgca acgtgactgt cgcccgagac ggcacttctg
     5341 gtctggcagc gattgtgcaa accgaattcg atctggttct gctcgattgc cagatgcccg
     5401 acatgagcgg ctatgacgtt gccaagctag cgcgcgaaca aaaaaacgaa ggcccatata
     5461 cccccatcat tgccatttcg gcggaaaccg atatggagca caagcaacgc tgtctggaaa
     5521 gcggtatgga cggcgtgtta ggcaaatctt ttccggttgc tggagagctg aagaaaatcc
     5581 tatcgctgtg gtgcaatttc gacctcgttg cggcgtcgtc gacagagatc gacgccatgg
     5641 aaaacatcga gctgcaaaca ttgttccgca ccatttctct ggaagatttg aacaccatgc
     5701 agacgctcgt tcaatccgat ataccaggtg tcggtcgcat ggcgcatcgc atgaaaggcg
     5761 ctgcgctgac catgggcgca gcggaaatcg ttgcaacgct tgagcgcatc gaagcaatta
     5821 tcgccgccaa cggtacgtca ttggcctcaa gcttgaggac tgaaataggt atcttgcggc
     5881 agcaatgtga cgtattggag aagtgcgccg actgagctta acaggccaat ttgttcggct
     5941 caatgcggca tgcaattgcg ctcgtattga attggggcgt aggtcaattt tggattaacc
     6001 ataattccga gcaatgtatc tggtgggggg caattcagac aaccgttcta cgtttccaac
     6061 gaagaaaaaa aatttggagg gtagttgatg caaggcagaa taggcgatga atggaagcga
     6121 gaaatatttg tcaccaaaac ccccgacctg caagactcag tggccgaact ttcgctgcag
     6181 ggcacccgga gaaatatcca acgatggctc gatcaaagcg gcatgtatcc gccagagggg
     6241 gcacctgagg aatattcatt ctacgcgttg gaatgttatc ccacagccca agatcgatct
     6301 gcgttttttc atcagttcat tgcttgttca aagcctcata ttggctatca gttactggcg
     6361 ctacttggtg gaacagggac cgttcgaagt gtttggacca ccaatttcga cggtctgacc
     6421 gcccgtgcct tgggagcagc aaacgtcgtt tgcgtggagg tcggcataga tacgccgcag
     6481 cggacagtgc gcgcgcaggg caataatgaa gtgcgtttgc tgtccttaca tggcggtttt
     6541 agatatggtt ttctccagcc gaatgggacg gcatgagatc aagaatccta atccgctgca
     6601 cggattggtc gaaaatcgtc cttgggatca ctcacttaca agttcaggct tttgcccgtc
     6661 aacggacgtg gccgtggtgt gcccaccaaa tttagcggct gcttttgaac agtttttgtt
     6721 tggacttgag caaccagcaa aacccaacca atccgagcgc gactacctac atgactatcc
     6781 tggatacacg acagtcataa ccgatcacga atcattcgat cttcatgact atgtcaaggc
     6841 atatgcggca cgcagaacgt cgtggagatt ggatgccctg gatgaaaaca ccgcatttgt
     6901 aggaattggt tacagccttg actctgatag atggccaatg ggaccgggcg aagaccatag
     6961 aacagtttta acttagttcg caatttaaca taatatttat tatacgaatt catatagcag
     7021 gcgcttcaac ccatataaca actcggctcg tgtcatcgcc aagatgcaga ccattgatga
     7081 gtcgagatcc tgaacaggtt cttatgacga taatgcggca aatacactgt ctctgatttg
     7141 ctcaaccggt ccgacgccgg tgatcttgag gtatttgggc gcgcccggct gcaccgactc
     7201 ggcccacttg ctgtagtaac cgaccagcac ttcagtctgt tcgtgataca ccgacagacg
     7261 cctcctgacg gtttcctcct tgtcgtcgtc gcgctgaacc aacgcttcac cggtgacgtc
     7321 atccttgccg tcaagcttcg gcggattgaa tttgacatga tagatgcgac ccgaagccgg
     7381 atgaacgcgg cgaccgctca tgcgctcgac gacggcgctg tccggtacgt caatttccag
     7441 aacgtagtca atctgaacac cggccttcct catggcatcg gcctgcggca ccgtgcgtgg
     7501 gaagccatcg aatagataac cattgacaca atcagcctgt tgtaggcgcc cctcgaccag
     7561 actgatgatg atgtcgtcgg acactaagcg acctgcatcc atgactctct tgacggcgat
     7621 acccagttcg gtgccggcag cgatggcagc acgcaacata tcgccggtcg aaatctgcgg
     7681 aatattgtat ttctccctga taaaagtggc ttgcgtacct ttaccggcac caggcgctcc
     7741 tagaaggatg agacgcattt ttttcctaaa ttgaattttg aattttgttg cgatggcgat
     7801 caaacgtacc ggcggattca gttgatccaa taactgtcat cgaaggacac cggcagcacc
     7861 tgctttgcac ggaacttacc acaaaatctt gctaaactgt cagaaatgaa agctacaacg
     7921 cagacattac cgctgccagc ggactatccg gcggcaccaa agtgcttgcg cacgcgttcg
     7981 agatcttcca gcgtatcgac gccggcttcc ggcagcagtg aagtaacatg cacagcaatt
     8041 gcataaccat gccataacac cctcaattgc tccagcgctt cgatacgttc cagcggcgaa
     8101 acggtcaacg tcgggtaaat ctgcaggaat tgattggtat aggcatacat accgatatgc
     8161 cgcagcaacg caaactcgtg gttgtgcggt tcggacaggt tttccttgga ctgtgcaaaa
     8221 tcgtcgcgat gccagggaat cgccgcgcgt gaaaaataca tcgcgccgcc atccttgttc
     8281 agcaccacct tcaccacatt tggattaaac atttccggca gcaattcgat gcggtgcgca
     8341 gccgtcgcca ttggtacgcc agccttgatc tgcgcggcta ccgccacaat caggccgggg
     8401 tcaatcagtg gttcgtcgcc ctgcacattg accactacgg catcatcaga cagatccaga
     8461 gctgccgcca cttcggcaac gcggtcagta ccggacagat gatcagcgcg cgtcatggcg
     8521 acatcgatac catgctgttc gcaggcggca tagatgtcgc ggtgatcggt cgccaccaca
     8581 atgcgcaccg cgcctgactg cgccgcccgc tccgccacgc gcacgatcat cggcttgccg
     8641 ccaagatcgg ccagaggctt gttcggcaaa cgcgtggaag caagacgcgc ggggatgatg
     8701 acaataaagg acataacaaa aaacaatcaa gaatttgaag gttccgatcc ggatgcagca
     8761 agcacggcaa cgccaaggtc acgcgcttct tcgacccaca tgatagggat gccgtcgcgg
     8821 accggataag ccagcctgtc g
//
LOCUS       DASRDH010000148         4769 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1191529,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000148 DASRDH010000000
VERSION     DASRDH010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 4769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4769
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1191529"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="VFA14_03380"
     CDS             <1..301
                     /locus_tag="VFA14_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HZS80457.1"
                     /translation="FKGLYFVLMGRLSPLDGIGPKDIHLEKLLTRASDGVVTEVVLAT
                     NFTNEGEATAHYISETLKARGLKASRLARGVPVGGELEYVDAGTIARAMLDRRAT"
     gene            459..1496
                     /locus_tag="VFA14_03385"
     CDS             459..1496
                     /locus_tag="VFA14_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZS80458.1"
                     /translation="MQLHLKRFAMTLTLFLTGYLTFSTSLFAQALEKPKVSIAVSGKS
                     LFYHLPLTLADKLGYFKDEGLQVHISDFANGAKALQAMEGGSVDVVSGAYEHTINMQA
                     KDQPVVAFVLQGRAPQIVLAVSNKTMHNYNDIADLKGKKIGVTALGSSTSIMASFVLA
                     KAGLKPTDVSFIAVGSAGEAASAIRSGRIDAIANVDPVITALEKKNEIKIIVDTRTLK
                     DTEDVFGGPMPAAALYAPEDFIKKHPNTTQALTNAIVRALKWIQKADLTAINKAIPDS
                     YVMGDRTLYIDIFMKMREAISPDGIFPDAGPVTALKMLEAFAPKLTDKKIDLSKTYTN
                     DFARKANAKYK"
     gene            complement(1671..2786)
                     /locus_tag="VFA14_03390"
     CDS             complement(1671..2786)
                     /locus_tag="VFA14_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013236080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HZS80459.1"
                     /translation="MLIQLAITGLLVALLPLTLTWVSGDTDKYRRLIWVTLFLTFDLI
                     MFGAFTRLTDSGLGCPDWPGCYGHANPLLAHEHISAAQEAMPTGPVTVAKAWIEMTHR
                     YLAMAVGVLIIALMMIAWWRWIKSRHRETRFRPGFPTLLFFFVCLQGAFGAWTVTMKL
                     QPVIVTLHLLFGLTLLAMLTWLVARENGHALVSAPGRSLAAPAALGLLLLVAQIALGG
                     WVSTNYAALACSDFPLCHGALIPLMDLENGFTLWRHLGKTAAGDYLPFPALTAIHWVH
                     RSFAFVVMAYIVWLAHKACNDAGLRKTGRWLLIVIALQLLTGISTIYLNWPLTIAVMH
                     NGGAALLLLLLVMLNYKTRFAPVAEAVPVSRTSLALP"
     gene            complement(2916..3524)
                     /locus_tag="VFA14_03395"
     CDS             complement(2916..3524)
                     /locus_tag="VFA14_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit I"
                     /protein_id="HZS80460.1"
                     /translation="MEKSKAFASEAQRKRGRWKLFLIIGVCAAPMMASYFTYYVIKPA
                     GRANYGALLDPRQYPIPRFGSAALDGKPAALEDYKGKWLMLQVAGGDCNEVCRTHLFA
                     MRQLRLMQGKEMERIERVWLITDARSPDTMLMREYDGTGMLRVDVSLLKAWLPTDRGT
                     MMEDHIYLIDPLGNLMMRFPRNADPNRMKKDISRLLRASAIR"
     gene            complement(3511..4230)
                     /locus_tag="VFA14_03400"
     CDS             complement(3511..4230)
                     /locus_tag="VFA14_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HZS80461.1"
                     /translation="MPIRCRVRWVPLAATLIVAVIGVSLGQWQSRRGDQKRAIQSRMS
                     ERWGAPVIDLVGAAVQLDIEQGEYRHVRVKGEFVSSWTSYLDNRPYRGVPGFHVLTPF
                     KIAGLDEAVLVKRGWTPRDIDDRTRISVLPPPVGEVEIEGVLRRDAGHLLQLGTAPAP
                     RAGAIMQNLDIPAYAAASGLKLAPFLIEQGGAMQDGLVRDWPAPSLGIERHRGYAVQW
                     YALAMMAIIFFVVTGVRSGKK"
     gene            4318..4518
                     /locus_tag="VFA14_03405"
     CDS             4318..4518
                     /locus_tag="VFA14_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006461519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin transmembrane helix small protein"
                     /protein_id="HZS80462.1"
                     /translation="MKVIIAISFLLILGSLAGALLFLMRDKGKSQRMVHALTLRIVFS
                     VLLFILLLAAHQFGLIQPSGIR"
     gene            complement(4537..>4769)
                     /locus_tag="VFA14_03410"
     CDS             complement(4537..>4769)
                     /locus_tag="VFA14_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent DNA helicase Rep"
                     /protein_id="HZS80463.1"
                     /translation="MYVGITRAQRSLHITWCKRRKRAREYTAGDSSRFIKEMRLDIGD
                     AVPREEERITPQNRLANLKALLQKPRPTLNGD"
BASE COUNT         1097 a         1466 c         1264 g          942 t
ORIGIN      
        1 tttcaagggt ttgtatttcg tgctgatggg acgcctgtcg ccgctggatg gcatcggccc
       61 caaggacatc cacctggaga aactactcac gcgcgccagc gatggcgtgg ttacggaagt
      121 ggtgctggcg accaacttca ccaacgaagg cgaagccacc gcgcactaca tcagcgaaac
      181 gctcaaagcc cgcggcctca aagccagccg cctggcgcgc ggcgtgccgg tgggcggcga
      241 actggaatac gtcgatgccg gtacgattgc acgcgccatg ctcgaccggc gcgcaacctg
      301 aacggtcaga tttatcacat ccaggacata tagaaaacaa tgaaatcttg tattcactgg
      361 agtatgatga cgcaagaatg acgccgttga gacaaggcaa gatcatttga aagcagttat
      421 tcgtccatct acgaagcaca ttgatagaag gagaacacat gcaacttcat ctgaagcggt
      481 tcgctatgac cctgaccctg ttcctgaccg ggtatctgac attcagcacc agtctgttcg
      541 cgcaggcgct ggaaaaaccc aaagtatcca tcgccgtcag tggcaagagc ctgttctatc
      601 acctgccatt gaccctggcc gacaaactgg gctatttcaa ggatgagggc ctgcaagtcc
      661 acatctccga tttcgctaac ggcgccaagg cgctgcaggc catggaaggc ggcagcgtag
      721 atgtcgtatc cggcgcttac gagcacacca tcaatatgca ggccaaagac cagcctgtcg
      781 tcgctttcgt cctgcaaggc cgcgcgccgc aaatcgtgct ggcagtcagc aacaagacca
      841 tgcacaacta taacgacatt gccgacctga agggcaagaa gatcggcgtg acggcgttgg
      901 gttcctcgac cagcatcatg gccagcttcg tcctggccaa ggcaggcctg aagccaaccg
      961 atgtttcctt cattgccgtc ggctcagcag gagaggccgc gtcagctatc cgttccggtc
     1021 gtatcgacgc aattgccaat gtggatccag tgattaccgc actggaaaaa aagaacgaga
     1081 tcaagatcat cgtcgatacc cgtacactga aagacaccga agatgttttc ggtggcccga
     1141 tgccggcagc ggcgctctat gcgccggagg actttatcaa aaaacacccg aacactaccc
     1201 aagcgctcac caacgccatc gtgcgtgcgc tgaaatggat acagaaggcc gacctgacag
     1261 ccatcaacaa ggcgattcct gatagctatg tgatgggcga ccgcacactg tacatcgaca
     1321 ttttcatgaa aatgcgcgaa gcgatttcgc cggatggtat cttccctgac gccggccctg
     1381 tgaccgcgtt gaagatgctg gaggcttttg caccgaagct gacggacaag aagatcgact
     1441 tgtccaaaac gtacaccaac gatttcgcca gaaaagcaaa cgccaagtac aaataatcga
     1501 tcgccgcaag ccgaacgggc gccttccttg gcgcccgttt ttcatttcct ggtcccgccg
     1561 cttttcatca tgatttgact tctcatttag cttcgattgt taaatgattt gtcagattaa
     1621 gtaatgacta attcacatga aatcaccgcc ctgtcgggcc tccattcaat tcacggcagg
     1681 gccaaactgg tccggctgac aggaacagcc tccgcaaccg gcgcaaatct agttttgtaa
     1741 tttaacatga ccagcaacag tagcagcaag gccgcaccgc cattgtgcat gacggcaatg
     1801 gtcaacggcc agttcagata aatggtcgat atgccggtga gcagttgcag agcgatcacg
     1861 atcagcaacc agcgtccggt tttacgcagc ccggcatcgt tgcacgcttt gtgcgccagc
     1921 cagacgatat aggccatcac gacgaaagca aacgaacggt gtacccaatg gatcgccgtc
     1981 aacgccggga acggcagata gtcaccggca gcggtcttgc ccagatgacg ccatagcgtg
     2041 aaaccattct ccagatccat cagtggaatc agcgcgccat gacacaatgg aaaatcgctg
     2101 cacgccagcg cagcgtagtt cgtactgacc cagccgccca gggcgatctg cgcaacgagc
     2161 aacagcagac ccagcgccgc tggcgccgcc agtgaccgcc ccggcgccga taccagcgca
     2221 tgcccgtttt cgcgcgcgac cagccaggtc agcatagcca gcagggtcag tccgaacagc
     2281 aaatggagcg tgacgatcac cggttgcagc ttcatggtca ccgtccaggc gccgaaagcg
     2341 ccctgcaggc agacaaagaa aaacagcagt gtcggaaagc ctggccggaa cctggtttcc
     2401 ctatggcgcg acttgatcca gcgccaccac gcaatcatca tcagcgcgat gatcagcaca
     2461 ccgaccgcca tcgccagata acgatgcgtc atctcgatcc acgctttggc aaccgtcacc
     2521 gggccagtgg gcatcgcctc ttgcgcagcg ctgatatgct catgcgccag cagcggattg
     2581 gcgtgaccat agcaacccgg ccagtcggga caacccagac cggaatccgt caggcgtgta
     2641 aaggcgccga acatgatcag gtcgaaggtc agaaacagcg tcacccaaat cagcctgcgg
     2701 tatttgtcgg tgtcgccaga aacccaggtc agggtcagcg ggagcagcgc gaccaacaat
     2761 cctgtgatgg caagttgaat cagcatgagg ttttagaacg tgtttacgat ccctccgagg
     2821 ccgtccagtc gccctttcaa gcggtctgcg gtttcgacct gcatcccatc ctgaaaaaac
     2881 gcccggatga ctcccggcaa gatcataaca cgttcttatc ttatggccga tgcgcgcagc
     2941 aatctgctga tatctttttt catcctgttg gggtcggcat tcctggggaa gcgcatcatc
     3001 agattgccca gcggatcgat gagataaatg tgatcctcca tcatcgtgcc ccgatctgtc
     3061 ggcaaccatg ccttcaacag gcttacatcc acacgcagca taccggtgcc gtcatattca
     3121 cgcatgagca tggtatcggg cgacctggca tcggtaatca gccagacgcg ctcgatacgc
     3181 tccatttcct taccctgcat caggcgcaac tggcgcatcg cgaacagatg cgtcctgcaa
     3241 acttcattgc agtcaccgcc agccacttgc agcatgagcc atttgccctt gtaatcttcc
     3301 agagcggccg gcttgccgtc gagcgcagcg ctgccaaacc gcgggatcgg gtactggcgc
     3361 ggatcaagca atgcgccgta gttggcgcgg cctgccggtt tgatgacata gtaggtaaag
     3421 taagatgcca tcatcggcgc agcgcacacg ccgatgatca aaaacagctt ccagcggcct
     3481 ctcttgcgct gtgcctcgga agcgaatgct ttactttttt ccactgcgca ctcctgtcac
     3541 cacaaagaaa atgattgcca tcatggccaa tgcataccat tgcacggcat aaccgcgatg
     3601 gcgttcgatg cccagcgacg gcgccggcca gtcacgtacc agcccgtcct gcatcgcgcc
     3661 accctgctcg atcaggaaag gcgcaagctt gaggccgctc gccgcagcat acgctggaat
     3721 gtcgagattc tgcatgatgg cgccggcacg cggcgcaggc gccgtgccta attgcagcaa
     3781 atggccagca tcgcggcgca gcacgccttc gatctcgacc tcgccgacgg gtggcggcaa
     3841 aacggaaatc ctggtgcggt catcgatgtc acgtggcgtc cagccgcgct tgaccagcac
     3901 ggcctcatcc agacccgcta tcttaaatgg ggtcaggaca tggaacccgg gcacaccgcg
     3961 ataaggccgg ttatcaagat agctggtcca ggaagaaacg aattcgccct tgacacgcac
     4021 atgccgatat tcgccctgct caatgtcaag ctgcaccgca gcgcccacca gatcgatgac
     4081 aggcgcgccc cagcgctcgc tcattctgga ctggatcgcc cgcttctggt cgccacgcct
     4141 gctctgccac tgccccagcg acacaccgat caccgcaacg atcagcgtcg ctgccagcgg
     4201 cacccatctc acgcggcatc tgatcggcat tacaatgtca cctcgtgcga tacggcaaca
     4261 gtctctacgc aatatgctgt cacgcaaacc gccgatgcag acacttccaa ccccaccatg
     4321 aaagtcatca tcgccatctc cttccttttg attctcggca gccttgctgg cgcgctcctg
     4381 ttcctgatgc gcgacaaggg caagagccag cgcatggtgc atgcgctgac attacgtatc
     4441 gttttttccg tgctgttatt catcctgctg ctggcggctc atcaattcgg cttgatccag
     4501 cctagcggca ttcgctgaac cttcatgcgc ctgctttcag tcgccgttca gcgtgggacg
     4561 cggtttctgc aacaaggctt tcaggttagc caggcggttt tgcggcgtga tccgctcttc
     4621 ctccctgggt acggcgtcgc cgatgtccag cctcatttcc ttgatgaagc gtgacgaatc
     4681 gccggccgta tattcgcgcg cgcgtttgcg tcgcttgcac caggtaatgt gcaggctgcg
     4741 ctgggcgcgc gtgatgccga catacatca
//
LOCUS       DASRDH010000149         5076 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_159261,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000149 DASRDH010000000
VERSION     DASRDH010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 5076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5076
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159261"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            51..749
                     /locus_tag="VFA14_03415"
     CDS             51..749
                     /locus_tag="VFA14_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HZS80464.1"
                     /translation="MNKVYPDAYSALSGIVKDGQTIAVGGFGLCGIPEALIAALRDSE
                     VRNLTAISNNAGVDGFGLGQLLTTRQIKKMIASYVGENKEFEHQYLAGELELEFTPQG
                     TLAEKLRAGGAGIPAFFTKTGIGTLVAEGKEIRAFDGVQYVMERSLVADVSLVKAHKA
                     DRAGNLVFNKTARNFNPNVAMAGKITVVEVEELVETGQLDPDQIHTPGIFVHRIVVNA
                     RPEKRIEQRTVSEK"
     gene            760..1410
                     /locus_tag="VFA14_03420"
     CDS             760..1410
                     /locus_tag="VFA14_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008333506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HZS80465.1"
                     /translation="MAWSRDEMAARAAKELQDGFYVNLGIGLPTLVANHVPGDIEVWL
                     QSENGLLGIGAFPTEDKVDADLINAGKQTVTTIPGSSFFSSADSFGMIRGGKIDIAIL
                     GAMQVSQQGDLANWMIPGKMVKGMGGAMDLVAGVGRVVVLMEHVAKNKDGSISQKILK
                     QCSLPLTGVGVVDRIITDLGVIDVTLQGLKLIELAGGVTREDVLANTEAALDVSAV"
     gene            complement(1435..1560)
                     /locus_tag="VFA14_03425"
     CDS             complement(1435..1560)
                     /locus_tag="VFA14_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS80466.1"
                     /translation="MDDVELDETRAAQPRPDVTAPYRELFGVFTDAIVNEDFEAN"
     gene            complement(1606..2421)
                     /locus_tag="VFA14_03430"
     CDS             complement(1606..2421)
                     /locus_tag="VFA14_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008542759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HZS80467.1"
                     /translation="MMWEIAYLCLGAFTGVLAGLMGVGGGMIMVPILTTLLQQQGVDA
                     THSLHLALGTSMATILVTSISSLYAHHRRQGVIWPLVGQLTPGIIVGAFIGTRIAVSL
                     PARVLTLFFTAMVVFVAIQMLTGTGASARPRPGRELPGVAGIVLVGTGIGAVSTLVAI
                     GGGIMTVPFLSWCNVRIQNAIGTSAAVGFPIALGGTLGYVINGWHVSTLPANTLGFVY
                     VPAWSWMVLASVFSARIGVALAHRLSARTVKRIFAVILIAIAVKLLLSSFWTG"
     gene            complement(2588..3316)
                     /locus_tag="VFA14_03435"
     CDS             complement(2588..3316)
                     /locus_tag="VFA14_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HZS80468.1"
                     /translation="MIELNKVSKWYGGFQVLTDCSTNVLKGDVVVICGPSGSGKSTLI
                     KTINGLEPFQKGVIMVDGVSVGDPKTRLPELRARIGMVFQSFELFPHLSVRENLTIGQ
                     VKVLGRSVDEASERGLKYLDRVGLIAHQDKFPGQLSGGQQQRVAIARALSMDPVAMLF
                     DEPTSALDPEMIGEVLDVMVGLAQEGMTMMVVTHEMGFARKVANRVIFMDQGMIVEDC
                     TKEDFFGSPRSERAHDFLAKIITH"
     gene            complement(3379..4074)
                     /locus_tag="VFA14_03440"
     CDS             complement(3379..4074)
                     /locus_tag="VFA14_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008331911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HZS80469.1"
                     /translation="MFASLDFDIIQRSWFYLITTGLKFTLTLTIAAMIGGIVLGTLLA
                     MMRLSGNKPLSFIATIYVNLIRSLPLVLVIFWFYFLMPFIGAWVIGASDPVQIDAFQS
                     ALVTFILFEAAYYCEIIRSGIQSIPRGQMFAGYAIGMNYWQMMSNVVLPQAFRNVAPI
                     LMTQTIVLFQDVSLVYVLGSVPDFVTSASKIAQRDGRLVEMYLFVAVVYFVLSFALSQ
                     FVKRFQNRVAIIR"
     gene            complement(4078..4827)
                     /locus_tag="VFA14_03445"
     CDS             complement(4078..4827)
                     /locus_tag="VFA14_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006461961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HZS80470.1"
                     /translation="MHYNWNWSIFWEESPDGIPYIDTLLTGLTWTIVTAALAWIMALI
                     LGAIVGTLRTTGKPWAVRIANAYIELFRNIPLLVQMFLWYFVMPELLPAALGNWLKSL
                     PEASFVTAFLALGFFTSARVAVQVSAGIDALPRGQRMAGAALGLTPVQIYCYVLLPMA
                     FRIIIPALTNEFAAIIKNSSVALTIGLMELTAASYSMREYTFQTFESLTGATLLYIII
                     AGIALLLARILEKAAAVPGYITTGSSSTGGH"
     gene            complement(4924..>5076)
                     /locus_tag="VFA14_03450"
     CDS             complement(4924..>5076)
                     /locus_tag="VFA14_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008116076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HZS80471.1"
                     /translation="QSPIPPKGINLHVPMSPQLQAVLAQPTDSGDPAAYAAVPEAQQQ
                     SSGKKK"
BASE COUNT         1172 a         1476 c         1447 g          981 t
ORIGIN      
        1 tgacggcaac ctgtgatgcc ctgatatttt cagacatcga aaggatacac gtgaataaag
       61 tctatcctga cgcgtacagc gcgttatctg gcattgtcaa ggatggccag acgattgccg
      121 tcggcggttt cggcctgtgc ggcattcctg aagcgctgat cgctgcgttg cgcgattccg
      181 aggtgcgcaa tctcacggcg atttccaaca atgccggtgt cgatggcttc ggtctcggtc
      241 aactgctgac gacgcgccag atcaaaaaaa tgatcgcctc ctacgttggt gaaaataaag
      301 aattcgagca ccaatacctg gccggtgaac tggaactgga attcactccg cagggtacgc
      361 tcgccgaaaa actgcgcgct ggcggcgctg gcattcctgc tttcttcacc aaaaccggga
      421 tcggcacgct agtggccgaa ggtaaggaaa tccgcgcatt cgacggcgtc caatacgtga
      481 tggaacgctc attggtggcc gatgtatcgc tggtcaaagc gcacaaggcc gaccgcgctg
      541 gcaatctggt cttcaacaag actgcgcgca acttcaaccc caacgtcgcc atggccggca
      601 agatcactgt ggtcgaagtc gaagaactgg tcgaaaccgg tcagctcgac ccggaccaga
      661 tccacacgcc tggtatcttc gtacaccgca tcgtagtcaa cgccaggccg gaaaagcgca
      721 tcgagcagcg cacggtttct gagaaataag gagaacgcca tggcatggag cagagacgaa
      781 atggcagcgc gcgcggccaa agaattacag gacggtttct acgtcaacct cggcatcggc
      841 ttgccgaccc tggtggcgaa tcacgttcct ggcgacatcg aagtctggct gcaatcagaa
      901 aacggcctgc tcggcatcgg cgcatttccg accgaagaca aggttgacgc cgacctgatc
      961 aacgctggca agcagaccgt caccacgatt cctggttcgt cgtttttcag ctcggccgat
     1021 tcgttcggca tgattcgcgg cggcaagatc gacattgcca tcctgggcgc gatgcaggtg
     1081 tcgcaacaag gcgacctggc caactggatg attcccggca agatggtcaa gggtatgggc
     1141 ggcgcgatgg atctggtggc tggcgtgggc cgcgtagtgg tgctgatgga gcacgtcgcc
     1201 aagaacaagg atggcagcat ttcgcaaaag atcctcaagc aatgcagttt gccactgacc
     1261 ggtgtaggcg tggtcgatcg catcatcacc gacctcggcg tgatcgatgt gacgctgcaa
     1321 ggtttgaagc tgatcgaact ggctggcggc gtgaccaggg aagatgtgct ggcgaacacc
     1381 gaggccgccc tggacgtcag cgccgtgtaa ctgaatggcg cagtcgaaca tggttcagtt
     1441 cgcctcgaaa tcttcgttta caatggcgtc ggtaaacacg ccgaacagtt cacgataagg
     1501 tgcagtgaca tctggacgcg gttgcgccgc tcttgtctca tcgagttcga catcatccac
     1561 agtcgatacc gagatacatg tccgcttgat ctaaacgtgc cactttcacc ccgtccagaa
     1621 cgacgaaagc agtaacttga cggcgatggc gatcagtatg accgcaaaga tccgcttgac
     1681 cgtccgcgcc gacagccggt gcgcgagcgc tacgccgatg cgcgcgctga acacgctggc
     1741 aagcaccatc cacgaccacg cgggcacata cacgaaacca agcgtattag cgggcagcgt
     1801 actcacgtgc cagccattga ttacatagcc gagcgtacca ccgagcgcaa tgggaaaccc
     1861 gactgccgcc gaggttccta tggcattctg gatgcgcacg ttgcaccagg aaagaaacgg
     1921 cacggtcatg atgccgccgc cgatcgccac caacgtcgag actgcgccaa tgccagtgcc
     1981 aaccaacacg atgccggcta caccgggcaa ttcccgacct ggccttggcc gcgcgctggc
     2041 accagtacca gtaagcatct gtatcgcgac gaagactacc atggccgtga agaacagggt
     2101 cagcacacga gccggcagac tcacggcgat gcgtgtgccg atgaacgcgc ccacgatgat
     2161 gcccggcgta agttgtccga ccagcggcca gatcacgccc tgccggcggt gatgcgcgta
     2221 aaggctcgat atcgacgtga cgaggatcgt cgccatcgaa gtgccaagcg caagatgcag
     2281 actgtgcgtc gcgtcaacac cctgttgctg cagcagcgtc gtcagtatcg ggaccataat
     2341 catgccgccg cccacgccca tcaaaccggc caatacgccc gtaaaagcgc caaggcagag
     2401 atacgcgatt tcccacatca tgtctgactt ctctgcttgt caaatccatg agcgtcgtac
     2461 aaccccgcct tgtgttccat acgtcctcca gacatggatt acatgcattg ctggtgccgg
     2521 gcctatggta aaagctggca gcaggaatgg tcaagcccat tctttttttg tcattcagcg
     2581 cagcgtatca atgcgtaatg atcttggcga ggaagtcgtg cgcgcgttcc gagcgtggcg
     2641 aaccgaagaa atcctccttc gtgcaatctt ccactatcat gccctggtcc atgaagatga
     2701 cacggttggc gaccttgcgc gcgaaaccca tttcatgggt cacaaccatc atggtcatac
     2761 cctcctgcgc caggccgacc atgacgtcca gcacttcgcc gatcatttca ggatcgagcg
     2821 cggatgtcgg ttcgtcgaac agcatcgcga ccgggtccat cgacaacgca cgggcaatcg
     2881 ccacgcgttg ctgctggccg ccagacagtt ggccggggaa tttatcctgg tgcgcgatca
     2941 gaccgacgcg atccagatat ttcaagcctc tttcgcttgc ctcatcgacg ctgcggccca
     3001 gcaccttgac ctggccgatg gtcagatttt cgcggaccga cagatgcggg aacagttcga
     3061 agctctggaa caccatgcca atgcgcgcgc gcagttccgg cagcctggtc ttcgggtcgc
     3121 cgacggacac gccgtcaacc atgataaccc ccttttgaaa cggctccagg ccgttgatgg
     3181 tcttgatcag cgtcgattta cccgaaccgg atggaccaca gatcacgact acgtcgccct
     3241 tgagtacgtt ggtgctgcaa tcggtcaata cctgaaaacc gccgtaccac ttgctgacct
     3301 tattgagttc aatcactttt atttctcctg attcctaaat acggtatcgg tcagccacgt
     3361 atcgccagca tgtgttggtt agcgaatgat ggcgaccctg ttctggaagc gcttcacaaa
     3421 ttgcgacagg gcaaagctga gtacaaaata gaccacggcg acgaacagat acatttccac
     3481 cagacggcca tcgcgctggg cgatcttgga ggcgctggtc acgaagtccg gcaccgaacc
     3541 cagcacatat accagcgaca cgtcctgaaa cagtacgatg gtttgcgtca tcaggatcgg
     3601 cgcgacgttg cggaacgcct gcggcagcac gacattgctc atcatctgcc agtagttcat
     3661 accgatggcg tagccggcaa acatctggcc gcgcggaatc gactgtatgc cggaacggat
     3721 gatttcgcag taatacgctg cttcaaacag aatgaaggtc accagcgccg actggaatgc
     3781 atcgatctgg acaggatcgc tggcgccgat gacccaggcg ccgatgaacg gcatcaggaa
     3841 atagaaccag aaaatcacca gcaccagcgg cagcgaacgg atcaggttga catagatcgt
     3901 cgcgatgaac gacagcggct tgttgccgga caggcgcatc atcgccagca gcgtgcccag
     3961 aacgatgccg cctatcatgg ccgctatggt cagcgtcagc gtgaacttca ggccggtcgt
     4021 gatcaggtag aaccacgaac gctgaatgat atcgaagtcg agactggcaa acatgactca
     4081 atgtcctcca gtactggaac tgccggtcgt gatataaccg ggtaccgcag ccgccttttc
     4141 caggatgcgc gccagcagca gcgcaatgcc ggcaatgatg atatacagga gggtggcgcc
     4201 ggtgagcgac tcaaacgtct ggaatgtgta ttcgcgcatc gaatagcttg ccgccgtcag
     4261 ctccatcaga ccgatggtca gcgccaccga actgttcttg atgatcgccg cgaactcgtt
     4321 ggtcagcgcc gggatgatga tgcggaaggc catcggcaac aatacatagc aatagatctg
     4381 cacaggcgtc aaaccgagcg ctgcgccagc catgcgctga ccgcgcggca aggcatcgat
     4441 gccggcgctc acctgcacgg caacgcgcgc ggaggtgaag aaccccagcg ccaggaaggc
     4501 cgtcacgaaa gaagcttccg gcaggctctt gagccagttg cccagcgcgg caggcagcag
     4561 ttccggcatc acgaaatacc agagaaacat ctggaccagc agtggaatat tgcggaacag
     4621 ctcgatgtaa gcgttggcga tcctgacggc ccagggtttg ccagtcgtgc gaagggtgcc
     4681 gacaatggcg ccgagaatca gcgccatgat ccaggccagc gcggcggtga cgatggtcca
     4741 tgtcaggccg gtcagcaagg tgtcgatata agggatgcca tcgggagact cctcccagaa
     4801 aatgctccag ttccagttgt aatgcatcga tgtccccatt tcggcccgtt gaacaaaacg
     4861 gacctctcat tttgctttta tttatgaaga cacggtacta catgtccagc acgctgctcc
     4921 ggtttatttt ttcttcccgg aagattgttg ttgcgcttcc ggcacggcgg cgtaagcagc
     4981 gggatcgccg gaatcggtag gttgcgccag gacggcctgt agttgaggac tcatcggtac
     5041 atgcaggttg atgcccttcg gagggatggg cgactg
//
LOCUS       DASRDH010000150         2591 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_462090,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000150 DASRDH010000000
VERSION     DASRDH010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462090"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(159..1214)
                     /locus_tag="VFA14_03455"
     CDS             complement(159..1214)
                     /locus_tag="VFA14_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS80472.1"
                     /translation="MNHLDILLPFGLPQAEMAPDLLRACQTPALAMLLARGKPSQPLQ
                     HFGPFARALPHEHWLSRQFGLTGANSADERGNSPPAAVPLLQTHHPSRCEGHWFVLQP
                     AHLHVARSHLMLTDTGQLALEETNARRLFQSALAPCEESGHTLLYIDAATWLLRADGW
                     AGLLTASPQAASGRNIDIWMPAGPGARAWRKLQNEVQMQWFSESLNEERERHGRKPVN
                     SLWLWGGGDAGALSSTIAYQAAFNLHGWSRAFNAAQQSDATLETILAAPGERGLLMLD
                     TLLEPGLAQEWEVWLQGMETLERDWFAPLRQALQDKRLNSLSLILSGQDKLLHLTASA
                     ASLRKFWVPASLAKLAV"
     gene            1397..>2591
                     /locus_tag="VFA14_03460"
     CDS             1397..>2591
                     /locus_tag="VFA14_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006464017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HZS80473.1"
                     /translation="MSLVKNLPFEWLVGIRYTRAGKRSGRNRFISFISLISMAGIALG
                     VAALIVVLSVMNGFQKEVRDRMLSVLAHIEIFDASGAMQDWQAVARQSLQNREVIGAA
                     PYVTAQAMMTRDDNVRGVVIRGVLPQEEPKVSDVAGRITQGSFSDLRPGEFNIVLGIE
                     LARSLRVGLGDKVTLIAPQGQVTPAGVLPRLKQFTVVGIFEAGHYEFDAALAFIQIED
                     AKRLFRLAAPSGVRLRIQDMLQAPQVAHALAQTLSGHFYLRDWSQQNSNWFAAVKTEK
                     RMMFIILTLIVAVAAFNLVSTLVMTVTDKQADIAILRTLGAAPGAIMKIFVIQGALIG
                     LIGTALGVGFGVLLALNIDVIVPAIERLFHVQFLPRNVYVISELPSDLIWSDVWTIGG
                     VAVLLA"
BASE COUNT          462 a          718 c          842 g          569 t
ORIGIN      
        1 gctcataacc cgaaggtcgc aggttcaaat cctgcccccg caaccaaacg tatctacaac
       61 gtcccccacg gcgcaagcca agggggacgt tgctatttct acccacgggc cacgtctctt
      121 gtcatcatgg ccgatgcaaa aatcaatgtc gcgctgactc ataccgccag ttttgccagc
      181 gaagctggca cccagaactt gcgcagcgac gcagcgctcg cagtgagatg cagcaacttg
      241 tcctgcccgc tcaggattag cgacagactg ttcagacgct tatcctgtag cgcttgccgc
      301 agcggtgcga accagtcgcg ctccagtgtt tccatgcctt gcagccagac ttcccattct
      361 tgggccaggc ccggttcaag cagggtatcc agcatcagca agccgcgttc gccgggagcg
      421 gcgaggatgg tttcaagggt ggcgtcggac tgttgtgctg cgttgaacgc gcgcgaccag
      481 ccgtgcaggt tgaaggcggc ttgataggcg atggtgctgg aaagggcgcc ggcatcgccg
      541 ccgccccaga gccagagtga attgaccggc ttcctgccat gcctttcacg ttcttcgttg
      601 agcgactccg aaaaccattg catctgcact tcgttttgca gtttgcgcca ggcccgcgcg
      661 cctggacctg ccggcatcca gatgtcgatg ttgcggccgc tggcggcttg cggcgatgcg
      721 gtgagcaagc cggcccagcc gtcggcgcgc aggagccagg tagcggcgtc gatgtagagc
      781 agcgtgtggc cgctttcttc acatggcgca agggcggact ggaagaggcg gcgtgcgttg
      841 gtctcctcca gtgccagttg accagtgtcg gtcagcatca gatgactgcg ggccacatgc
      901 aggtgcgccg gttgcaggac aaaccagtgg ccttcgcacc gcgatggatg gtgtgtttgc
      961 agcagtggga cggcagcggg cggactgttg ccgcgctcat ccgcgctgtt ggctccggtc
     1021 agtccgaact ggcggctgag ccagtgttca tgcggtaggg cgcgggcaaa tggaccgaaa
     1081 tgttgcagtg gctgggatgg tttaccgcgt gccagcagca tggcaagggc aggggtttgg
     1141 cacgcgcgca gcaggtcggg cgccatctcg gcctgcggca ggccgaaagg gagcaggatg
     1201 tcgagatgat tcatgccgcg attgtaggtg aaaacctttt acgcgatgtt ttctgtcgtg
     1261 aagacctatc cggtttcgac gtgctgcgcc gacggtgaaa gatatcggag agtgtggcaa
     1321 actggcaggc ctgccagttt ttccgcatgg ttgcggtcct tgcgctaatc tttcttcgtg
     1381 aaaagtgagt ccggatttga gtttggtcaa gaatttacct tttgaatggc tggtcggcat
     1441 ccggtatacg cgcgctggca agcgtagcgg ccgtaatcgc ttcatttcct tcatttccct
     1501 gatttccatg gccggcatcg cactcggcgt tgccgcgctg atcgttgtgc tgtcggtgat
     1561 gaacggtttc cagaaagaag tacgcgaccg catgctgtcg gtgctggcgc atatcgaaat
     1621 attcgatgct tccggcgcca tgcaggattg gcaggcggtg gccaggcagt ccttgcaaaa
     1681 cagggaagtc atcggcgctg cgccttatgt gacggcgcag gcgatgatga cacgtgacga
     1741 caatgtgcgc ggcgtggtca ttcgcggcgt gctgccgcag gaggaaccga aggtatcgga
     1801 tgtcgccggg cggatcacgc agggcagctt ctccgatctc aggccgggtg agttcaacat
     1861 cgtgctgggc atcgaactgg cgcgcagtct gcgcgtgggg ctgggcgaca aggtcacgct
     1921 gattgcgccg caggggcagg tgacgccagc cggggtattg ccgcgcttga agcagtttac
     1981 ggtggtcggc attttcgagg cggggcatta tgagttcgat gctgcgctgg cgtttatcca
     2041 gatcgaagat gccaagcgat tgttccgtct cgctgcgcca tccggcgtgc gcctgcgcat
     2101 tcaggatatg ttacaggcgc cgcaggtggc tcatgcgctg gcgcaaacgc tcagcggtca
     2161 tttctatctg cgtgactggt cgcaacagaa cagcaactgg ttcgccgctg tcaagactga
     2221 aaagcgcatg atgttcatca tcctgacgct gatcgtcgcc gtggcggcct tcaacctggt
     2281 gtcgacgctg gtgatgacgg tgaccgacaa gcaggccgat atcgccatcc tgcgcacgct
     2341 cggcgcagcg cccggggcta tcatgaaaat cttcgtgatt cagggtgcgc tgatcggctt
     2401 gatcggtact gcgttgggcg ttggtttcgg cgtgctgtta gcgctcaata tcgacgtcat
     2461 cgtgccggcc atcgagcgtc tttttcacgt acagttcttg cccaggaatg tttatgtcat
     2521 ttccgaatta ccatccgacc tgatctggtc ggatgtgtgg accataggtg gcgtggcggt
     2581 gctgctggcc t
//
LOCUS       DASRDH010000151         8511 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1531144,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000151 DASRDH010000000
VERSION     DASRDH010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 8511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8511
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1531144"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="VFA14_03465"
     CDS             complement(<1..483)
                     /locus_tag="VFA14_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HZS80474.1"
                     /translation="MYFIALIAFLIFLPILGIAGAWLHLDAAGIDAMRQQAQTVLPAY
                     LATSGWLALCVTVGVIIVGAATAATISLFEFRGKRFFSWALLLPMAMPAYVSAYAYTD
                     FLQYAGAVQSSLRAIFPGFRIDVRSLSGAIFLFIFTLYPYAYLLARSALSERGVHLME
                     A"
     gene            607..1743
                     /locus_tag="VFA14_03470"
     CDS             607..1743
                     /locus_tag="VFA14_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008330902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZS80475.1"
                     /translation="MFLQIDQVSIRYPRAAIPAVQSISLALQSGQLGVLIGPSGSGKT
                     TLLRAIAGLEHPQSGRILLDRQMLDGPGVHVAAEQRRIGMVFQDFALFPHLSSAANIG
                     FGLRTGSRQQRRRRVRDMLELVGLDDLQDKFPHQLSGGQQQRVALARALAPQPRLLLL
                     DEPFSSLDVELRERLAEDVRRILKQAGTTALMVTHDQMEAFAIGDMVGVMQQSCLEQW
                     DHPYALYHRPVTRFVADFIGHGIFVPGTLVRERKTDVIKTPLGRLQDVTQALPHNDPC
                     RREFDVLLRSDDIVHDDLSPFKARIVRKSFRGADFLYTLQLDDGTQVLSLVPSHHDHA
                     IGEWIGIRLELDHVVVFPRLNANQDPMVDAVQGMKQNLIDCQRA"
     gene            1762..5469
                     /gene="putA"
                     /locus_tag="VFA14_03475"
     CDS             1762..5469
                     /gene="putA"
                     /locus_tag="VFA14_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011517917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HZS80476.1"
                     /translation="MSPFQSFVESIPHISPLRKAITEATRRPEAEAVQALLDLAHLPH
                     GMAQQTQALANRIARILRERKASPGRAGLVQNLLQEYALSSQEGIALMCLAEALLRIP
                     DAATRDALIRDKIANGQWQTHLGKSRSIFVNAATWGLLITGKLVSTHSESGLSATIGR
                     LVSMGGEALIRKGVDMAMRMMGEQFVTGETIDQALSNARIREAEGFTYSFDMLGEAAL
                     TEYDAERYLRDYEAAIHAIGKASAGRGIYDGPGISIKLSALHPRYSRSQFDRVMTELY
                     PVVLRLCALARRYEIGLNIDAEEADRLEISLDLLEKLCTEPQLRDWDGLGFVVQAYQK
                     RCPFVIDYVIDLASRSKRRLMVRLVKGAYWDAEIKRAQIDGLDDYPVYTRKLYTDISY
                     VACARKLLDAPEEVYPQFATHNAHTLAAIYQLAEPARHHPQQYEFQCLHGMGESLYEQ
                     VVGSSDAGRLGRRCRIYAPVGTHETLLAYLARRLLENGANTSFVNRIADPTVPIDELV
                     RDPVQQVKMQAAEEGMLGLPHPAIASPRHLYGPARLNSRGLDLADESQLQELSHTFQK
                     TATVRFFAGLSVDTAGGESRPVHNPANRDDIVGHVRDSTPAEVDLAIEQAVSEASRRV
                     VAPEERAAILNTAADRLEADMPRFMALLIREAGKTALNAIAEVREAVDFLRYYSHQVK
                     EQFNNETHRPLGPVVCISPWNFPLAIFIGQISAALAAGNTVLAKPAEQTPLVAAEAVR
                     ALWAAGVPRAALQLLPGSGEVIGAQLVGDRRVQGILFTGSTEVARTIQRTISDRTDLS
                     GKAIPLIAETGGQNAMVVDSSALAEQVVIDVLGSAFDSAGQRCSALRILCVQEDAAER
                     ILEILLGAMAELRIGNPDRLAVDVGPVIDEEARANIQRHIDAMRSRGCKVHQVAGRTL
                     HSLDRGTFVLPTLIEIGSISDVEREVFGPVLHFVRYRRDELDGLLSQINGTGYALTMG
                     LHTRIDETIARVVDTAHAGNLYVNRNMVGAVVGVQPFGGEGLSGTGPKAGGPLYLYRL
                     LSHRPHDVMKHVLMNPFRANGNPEARSSFRALTQWASKKNPRLATVCEAFAEESRSGV
                     SELLPGPTGERNVYTLVPRRAVLCLASSADDLLVQIAAVLSVDSRAILPVASEQILHD
                     MPVEVRGCVSVAKDWSADEVTFDAVLLHADANELTRVRQQLACRRGPIVAVERMNPGE
                     VRVPLDRLIVERALSVNTAAAGGNASLMTIG"
     gene            complement(5670..6611)
                     /locus_tag="VFA14_03480"
     CDS             complement(5670..6611)
                     /locus_tag="VFA14_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011660006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZS80477.1"
                     /translation="MGSIDRKDSTPQLLNRLRMRQIALMLAIQEQRTLRGAAAQLGMS
                     QPAATKMLHDLEDALGLRLFERVGRGLRINGAGGRVLDYCQSLRGSTEALNRELLELR
                     QGSAGRLAVGSIMAASPGRLTDALLGIKRHSPLLSIEVAVDTSDRLLAQLREGVLEVV
                     VGRMADEHGGDYRFRPLDNEELVIVAGLVHPLARKRMISLEALLPYPWVLQPPGSPMR
                     ALIDLEFRDRGLTLPRGLIETGSILTSINLVRSSRMVAVIPAMVARSNAEHGLLRVLP
                     FHFQRRLEAYGSIVRRDHALSRPARQFLTLLHQDREG"
     gene            6781..7815
                     /locus_tag="VFA14_03485"
     CDS             6781..7815
                     /locus_tag="VFA14_03485"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016451315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050897 - cobalt ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HZS80478.1"
                     /translation="MSALEKPVVALTLGDPAGIGAELIARLLAKPEATQHANIVLVGD
                     PWLWQEGQHIAGVQVATSPIDSLAAMRGRAHTSHAAFLAVNTVAPEAVHRSMAEAPGG
                     RSVLQVLNQCMDATLAKQIDAICFAPLNKQAMKLGGMKHEDELHHFAKYLGVTGYFCE
                     FNTLGNLWTSRISSHVPLKDVASYLSVERITQAAELIYQSLLDNGFETPRVAIAGFNP
                     HSGDGGTCGREEIDIIEPAVRALQTRQWPTHYPFHGPFPADTIFLKAQAGAYQAIVTM
                     YHDQGQIAIKLLGFSRGVTVQGGLPIPITTPAHGTAYDIAGQGKANVEATWQAFRIAC
                     RMGAARLSHS"
     gene            7941..>8511
                     /locus_tag="VFA14_03490"
     CDS             7941..>8511
                     /locus_tag="VFA14_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016451314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
                     /protein_id="HZS80479.1"
                     /translation="MKIKSARAHVYEWKGKTVPPQGNFCSNAMDLLYSRQETMSTFRF
                     HSWTVVEIETDDGIVGVGNAALAPRIAKAIIDEHLAPLIIGQDPWDYECLWQRMYRST
                     HAWGRKGVAMAAISAADLAIWDILGKSVNKPVFKLLGGRTKEKIPCYYSKLYRTNLKV
                     MQEEAQKFKDQGFKAFKMRFGYGPAHLQDG"
BASE COUNT         1780 a         2680 c         2471 g         1580 t
ORIGIN      
        1 cgcctccatc agatgcacgc cccgctcact gagcgcactg cgcgccagta gataagcata
       61 gggatacaag gtaaaaatga acaggaatat cgcacccgac aaactacgca catcgatgcg
      121 aaagccggga aagatcgcgc gcaaactact ctgcaccgct cccgcgtact gcagaaaatc
      181 cgtgtacgca tacgccgaca catacgccgg cattgccatc ggcaacaaca gcgcccagga
      241 aaagaaacgc ttgccgcgaa actcgaacaa actgatcgtc gccgccgtcg cggcgccgac
      301 gatgatgaca ccgactgtca cgcacagcgc cagccagccc gaagttgcca gatacgccgg
      361 cagcacggtt tgcgcctgct gccgcatggc gtcgataccc gccgcatcga gatgcagcca
      421 cgcaccggca ataccgagga taggcaggaa aatcagaaac gcgatcagag caatgaaata
      481 catcgggcgc cagtcaaaaa atgcatggat gcaaacaaat atcaggaata acagcggtcg
      541 ctcgcgacga tacgctacac ttgccgaagt tcatgcaaat gagaattgta acgataaagg
      601 tctctgatgt tcctgcaaat cgatcaagtc agcatccgct atccgcgcgc cgccatacct
      661 gcggtgcaat cgatatcgct ggcattgcag tccggtcagc taggcgtact gatcggtcct
      721 tccggtagcg gcaagaccac cctcttgcgc gcgatcgccg gacttgagca tccacaatcc
      781 ggccgcattc tgctcgaccg acaaatgctc gatggccccg gtgtacatgt tgcggccgaa
      841 cagcgccgca tcggcatggt gttccaggat tttgccctgt ttccgcatct gagcagcgcc
      901 gccaacatcg gtttcggcct gcgcacgggt agcaggcagc agcgccgccg gcgcgtgcgc
      961 gacatgctgg aactcgtcgg actcgacgac ctgcaggaca aatttcctca tcaactctcc
     1021 ggcggccagc agcagcgcgt cgccctggcg cgcgcgctag cgccacaacc gcgcctgtta
     1081 ctgctggacg aacccttctc cagcctcgat gtcgaattac gcgagcgtct ggctgaagac
     1141 gtgcgccgta tactcaagca agccggcacc actgccttga tggtgacaca cgatcagatg
     1201 gaagcatttg ccatcggcga catggttggc gtgatgcagc aaagctgtct ggaacagtgg
     1261 gaccatccct atgcgctgta tcaccgcccc gtgacgcgct tcgtggccga cttcatcggt
     1321 cacggcatct tcgtaccggg cacactagtg cgcgagagga aaacagacgt catcaagacg
     1381 ccgctcggcc gcttgcaaga tgtgacgcaa gccttgccgc ataatgatcc ctgccgacgg
     1441 gagttcgatg tattactgcg ttccgacgac atcgtccatg acgacctgtc gcccttcaaa
     1501 gcgcgcatcg tgcgcaaatc attccgtggc gcagacttcc tatatactct gcaactcgat
     1561 gacggcaccc aggtgctatc gttggtgcct tcgcatcacg accacgccat cggcgaatgg
     1621 ataggcatac ggctggaact cgatcatgta gtggtttttc cacggctgaa tgccaatcag
     1681 gatccgatgg tcgatgcagt ccagggaatg aagcaaaatt taattgattg ccagcgagca
     1741 tagcggcagc tagagcgtgt tatgtcacca ttccaatcat tcgtcgagtc aatcccccac
     1801 atttcaccac tgcgcaaggc tatcactgaa gccacccgcc ggcccgaagc cgaagcagtt
     1861 caggctctgc ttgatctggc gcacctgccg cacggcatgg cacagcagac tcaggcgctg
     1921 gcaaacagga tcgcccgcat cctgcgcgag cgcaaagcct ctccaggacg ggccggcctg
     1981 gtgcagaacc tgttgcagga atacgcgttg tcctcgcaag aggggattgc cttgatgtgc
     2041 cttgccgagg cgctgcttcg catcccggat gcagccacgc gcgatgcgct gatccgggac
     2101 aagatcgcga acgggcaatg gcagacccat ctcggaaaga gccgatccat cttcgtcaac
     2161 gcggcgacat ggggactgct catcacgggc aagctggtct cgacgcacag cgagtccggg
     2221 ttgtccgcga cgatcggacg cctcgtgagc atgggagggg aagctctcat ccgcaaaggc
     2281 gtagatatgg ccatgcgcat gatgggagaa cagttcgtca caggcgagac gatcgaccag
     2341 gccctgtcca atgcgcgcat tcgcgaagcc gaaggtttca cgtactcctt cgatatgctc
     2401 ggtgaggccg cgctcaccga gtacgacgct gaacgctacc tgcgcgacta cgaagccgcg
     2461 atccacgcca tcggcaaggc atctgcaggt cgcggcatct atgatggacc cggaatctcg
     2521 atcaagcttt ccgcgctgca tccgcgctac agccgatcgc aattcgatcg cgtgatgacg
     2581 gaactctacc cggtcgtcct gcggctgtgt gcgctggcta gacgctacga aatcggcttg
     2641 aatatcgatg ctgaggaggc ggaccggctt gaaatctcgc tggacctgct cgaaaaacta
     2701 tgcaccgagc cgcagctgcg ggactgggac ggcctgggct ttgtagtgca agcatatcag
     2761 aagcgctgtc ctttcgtcat cgactacgtg atcgatctgg ccagtcgcag taagcggcgt
     2821 ttgatggtcc gtctggtcaa gggcgcctac tgggatgcgg agatcaaacg cgcccagatc
     2881 gacggcctgg atgactatcc ggtatacacg cgcaagctct atacggacat ttcctatgtg
     2941 gcgtgcgcac gcaagctcct ggacgcaccc gaggaggtct atcctcagtt tgcgacgcac
     3001 aacgcgcaca cgctcgcggc catctaccaa cttgccgaac cggccaggca ccatccgcag
     3061 cagtatgagt tccagtgcct gcacggcatg ggagagtcgc tgtacgagca ggtcgtcggc
     3121 tcatccgatg ccggcagact cggccgccgt tgccgcattt acgcgcccgt gggcacgcac
     3181 gaaacgctgc ttgcctacct ggcgcggcgc cttctcgaaa atggggctaa cacctccttc
     3241 gtcaatcgca ttgccgatcc gactgtgccg atcgacgagc tggtgcgtga tccggtgcag
     3301 caggtgaaga tgcaagccgc ggaagaagga atgctgggtc tgccacatcc cgcgattgca
     3361 tcgccgcggc atctgtacgg gcctgcccgc ctgaattcgc gcggcctcga cctggccgac
     3421 gaatcacagc tacaagagct gtcccacaca ttccaaaaga cggctaccgt gagattcttt
     3481 gcaggactgt cggtcgatac cgccggtggt gaatcgcgac cggtacacaa cccggcgaac
     3541 cgcgacgaca tcgttgggca cgtgcgtgac agcacgcctg ccgaggttga cctagcgatc
     3601 gagcaagccg tgtcggaggc gtcgcgccgg gtagtcgccc cggaggagcg ggctgcgata
     3661 ttgaacacag cagctgatcg tcttgaagca gatatgccaa ggttcatggc gctcctgatt
     3721 cgcgaagctg gtaagaccgc gctcaatgcg attgctgaag tacgcgaagc agtggacttt
     3781 ctgcgatact actcgcacca ggtcaaagag caattcaaca atgaaacgca tcgcccgctc
     3841 ggccccgtag tttgcatcag cccgtggaac ttccccttgg ccatattcat cggccagatc
     3901 tcagcggcat tggctgccgg caacacagtg ctggccaaac cggccgaaca gaccccgctc
     3961 gtcgccgcgg aagcagtgcg tgcgctctgg gccgccggcg taccgcgcgc cgccctgcaa
     4021 cttctgccgg gaagcggcga agtcatcggg gcacagcttg tcggtgaccg gcgcgtacag
     4081 ggtattctat tcaccggctc aacggaagtt gcgcgcacta tccagcgcac catcagtgat
     4141 cgcacggatc tcagtggcaa ggccattccg ctgatcgccg agacgggggg acagaacgcg
     4201 atggtggttg actcctcggc gctggccgag caggtagtca tcgacgttct tggttcggcg
     4261 ttcgacagtg caggccagcg ctgctcggcg ctgcggattc tgtgcgtgca ggaggacgct
     4321 gctgaacgca tccttgagat actgctcggt gcgatggccg aactgcgcat cggcaacccg
     4381 gatcgcctgg ccgtggacgt cggccctgtc atcgatgagg aagcgcgcgc gaacatccaa
     4441 cgacatattg acgcgatgcg aagcaggggc tgcaaggtcc accaggtcgc aggccgcacg
     4501 ctgcattctt tggaccgtgg caccttcgta ctgcccactt tgatcgaaat cggcagcatc
     4561 tccgacgtgg agcgcgaagt atttggacct gtcctgcact tcgtgcggta tcggcgtgat
     4621 gagctggacg gccttctgag ccagatcaat ggaacggggt atgcattgac gatggggctg
     4681 cacacgcgca tcgatgagac catcgcccgc gtggtagaca ccgcccatgc cggcaacctc
     4741 tacgtgaatc gaaacatggt cggcgcggtg gtgggcgtgc agccgttcgg cggcgagggc
     4801 ttgtccggca ccggcccgaa agcaggcggg ccgctatatc tgtatcgact gctctcgcat
     4861 cgaccgcacg atgtcatgaa gcacgtactc atgaacccat ttcgcgccaa tgggaatcca
     4921 gaggcacggt cgagcttccg tgcgctgacg caatgggcct cgaagaagaa tccgcgactt
     4981 gccacggtgt gcgaggcatt tgccgaggag tcccgtagcg gagttagtga actgttgccc
     5041 gggcctaccg gcgagcgaaa cgtctacaca cttgtacccc ggcgcgccgt tctgtgcctg
     5101 gccagcagtg ccgacgacct gctcgttcag attgcagcag tcctgtccgt ggacagtcgc
     5161 gcgatcctgc cggttgcttc agagcagatt ctgcacgaca tgccggtaga ggtgcgaggt
     5221 tgcgtttctg tagccaaaga ctggagcgcc gacgaagtca cctttgatgc cgtactgctg
     5281 catgcggatg ccaatgaact cacgcgcgtg cgtcagcagc tagcctgccg ccgcgggccg
     5341 atcgtcgcag tcgaacgcat gaacccgggc gaggtgcgcg ttccactcga tcgcctgatc
     5401 gtggagcgtg cgctcagtgt caacacggct gctgccggtg gcaatgcctc tctcatgacg
     5461 atcggatagg ttttatgctg ctgctcgtga ctatttcttc tttttgatcg tcttttcaac
     5521 ctcctcgggc atgatttcca gtacgttggc gatctcttct gtcgtcgtat ttttccaatc
     5581 acccggacgg catctttcat cactgcaagt cgatatgacc tgcccccaag gtagggcttc
     5641 cgtatgaagc tcggcgatcg attcacactc tacccctccc tgtcctggtg cagtagtgtc
     5701 agaaactgcc gcgccggtcg gctgagggca tggtcgcggc gcacgatgct gccatatgct
     5761 tccagccgac gctgaaaatg gaacggcagc acgcgcagca agccatgttc agcattgctg
     5821 cgcgccacca tggccgggat caccgccacc atccgcgaac tgcgcaccag gttgatgctg
     5881 gtaagaatcg aacccgtctc aatcaggccg cgcggcagcg tcagaccccg gtcacggaac
     5941 tctaggtcga tcaatgcgcg catggggcta cccggcggct gaagtaccca ggggtagggc
     6001 agaagggcct ccagcgagat catgcgcttg cgcgccagcg gatgcaccaa gccggcgacg
     6061 atgaccagtt cttcgttgtc cagaggacga aaccggtagt cgccgccgtg ttcgtcggcc
     6121 atgcggccca ccaccacctc cagcacgcct tcgcgcaact gcgccagcag ccggtcgctg
     6181 gtatccaccg ccacttcgat ggacagcaag ggactgtggc gcttgatgcc cagcagcgcg
     6241 tccgtcagcc ggcctggcga tgccgccatg atgctgccca ccgccagtcg gccagcactg
     6301 ccctgccgca gttcgagaag ctcccgattg agcgcctccg tgctaccacg caagctctgg
     6361 caataatcca gcacccgccc gccagctccg ttgatccgca agccccgacc cacgcgctcg
     6421 aacaagcgca ggcccaatgc atcctccaga tcgtgcagca ttttggtagc tgcaggctgg
     6481 gacatgccca gttgtgccgc cgcaccgcgc agcgtacgct gttcctggat ggcgagcatc
     6541 agcgcaatct ggcgcatgcg caggcggttg agcagttgcg gtgtgctgtc tttgcggtcg
     6601 atcgatccca tcactgaccc tggtaattga ttgccttggg ttatcaattt atcaatttct
     6661 ttcagtttac ggcaagggac tctgttccta ccatgctcat caaacacaaa aagcgaagca
     6721 ggcgaaacat gcaagcaagg cttcggcaac attacatcat gcttgattga aaggcagcat
     6781 atgtccgccc ttgagaaacc tgtcgtcgcc ctgaccctgg gcgatcctgc cggcatcggt
     6841 gccgaactca tcgcccgcct gctagccaaa cccgaggcca cgcagcatgc caacatcgtg
     6901 ctcgtgggcg acccctggct gtggcaggaa ggtcagcaca tcgctggcgt acaggtcgcc
     6961 accagcccca tcgacagtct agccgccatg cgcggccgcg cacatacctc gcatgccgcc
     7021 tttctggccg tcaacaccgt ggcgcccgaa gccgtgcatc gcagcatggc tgaagcgccc
     7081 ggcggccgct cggtactgca agtgctgaac cagtgcatgg atgcgacgct ggcaaagcag
     7141 atcgatgcca tctgcttcgc acccttaaac aagcaggcca tgaagctggg cggcatgaaa
     7201 catgaggacg agctgcacca cttcgccaag tacctgggcg tgacgggcta cttctgcgag
     7261 ttcaacacgc tgggcaatct gtggacctcg cgcatttcct cgcacgtgcc actcaaagat
     7321 gtggccagct acctgagcgt agagcgcatc acgcaggcgg cggagttgat ctaccaatcc
     7381 ctgttggaca atggtttcga gactcccaga gtggcgatcg cgggctttaa cccgcacagc
     7441 ggcgacggcg gcacctgcgg gcgcgaggaa atcgatatca tcgaaccagc cgtgcgcgcc
     7501 ttgcagaccc ggcagtggcc tacgcactat cctttccacg gccctttccc ggctgacacc
     7561 atctttttga aggcgcaggc cggcgcgtac caagctatcg tgaccatgta ccacgaccag
     7621 ggccagatcg cgatcaagct gcttggattc tcgcgcggcg tgacggtgca gggtggcctg
     7681 ccgataccga tcaccacgcc ggcacatggc acggcctacg acatcgccgg tcagggcaag
     7741 gccaatgtgg aagccacctg gcaagccttc cggatcgcct gccgcatggg cgccgcgcgc
     7801 ctgtcccact cgtaaatcgc tgacaacgac aggagacaga tgcggtgcat ctcacaacca
     7861 caattgcagg agcagccggt aacccgggcg gcgcctgcct tgatcattcc ccactccccc
     7921 aacgactcct gaaaacactc atgaaaatca aatctgcacg tgcccatgtt tatgaatgga
     7981 agggtaagac cgttccgccc caaggcaatt tctgctccaa cgccatggat ctgctgtatt
     8041 cccgacagga gaccatgagc accttccgct tccattcctg gacggtagtg gagatcgaaa
     8101 ccgacgacgg catcgtcggt gtgggcaacg cggcgctggc acccagaatt gccaaggcca
     8161 tcatcgacga acacctggcg ccgctgatca tcggccagga tccgtgggac tacgaatgcc
     8221 tgtggcaacg catgtaccgc agcacccacg cttggggccg caagggcgtg gccatggccg
     8281 ccatctctgc cgctgacctg gctatctggg acatcctagg caagagcgta aacaagcccg
     8341 tgttcaagct gctgggcggc cgcaccaagg agaagatccc ctgctactac agcaagctgt
     8401 accgcaccaa cctgaaggtc atgcaggaag aggcgcagaa gttcaaagac cagggcttca
     8461 aagccttcaa gatgcgcttc ggctatggcc ccgcgcatct gcaagacggc g
//
LOCUS       DASRDH010000152         6105 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1437807,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000152 DASRDH010000000
VERSION     DASRDH010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 6105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6105
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1437807"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..2190)
                     /gene="rpoD"
                     /locus_tag="VFA14_03495"
     CDS             complement(19..2190)
                     /gene="rpoD"
                     /locus_tag="VFA14_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006462953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HZS80480.1"
                     /translation="MPVAKAASEARGVDAMIVSGVSQMTDAAALAAIDTSGYILPSVK
                     VPGRRGRKPKEFQPENDEIAALNAVERAELKAVDKARAKDRKAKEKALLKDAFSSDTE
                     ATEEELERRRQKLKALIKHGKERGFLTFSEINDHLPENIVDPEAIEGIIGTFNDMGIA
                     VYEHAPDAETLLLSDSVASDDDAEAAAEAALSTVDSDFGRTTDPVRMYMREMGSVELL
                     TREGEIEIAKRIEDGLKDMIQAISACPTTIDEILAVAQKIRNDEIRVDEIVDGLVDLN
                     EGEVVVTAAASDGDEHEEDDAEAEEEGSDSSSSGAAGFSAEQLEQLKRDALDKFTIIS
                     VQFDNMRKAFEKEGYNSKPYVKAQEVISNELLGIRFTAKVVEKLCDTLRSQVDQVRQI
                     ERQILDVAVNRCNMPRAHFVKVFPGNEINLDWVDGEVDANHSYSVVLGRNIPAIKELQ
                     KKLIDLQARVVLPLPDLRGINKKMGAGEKKARMAKREMTEANLRLVISIAKKYTNRGL
                     QFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMI
                     ETINKMNRISRQILQETGAEPDPATLAIKMEMPEDKIRKIMKIAKEPISMETPIGDDD
                     DSHLGDFIEDNNTLAPSDAALHASMRGVVKDILDSLTPREAKVLRMRFGIEMSTDHTL
                     EEVGKQFDVTRERIRQIEAKALRKLRHPSRSERLRSFLDNN"
     gene            2189..2572
                     /locus_tag="VFA14_03500"
     CDS             2189..2572
                     /locus_tag="VFA14_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS80481.1"
                     /translation="MAIFDSGFLLVTEAFDCTEAVFGLARDKFFFVDATAAPALDFFV
                     GDAIALAIVTTTLPVRLTAVVRFGADRATAGDCLTFCLIADVLFFTEVFAVFFIAFAI
                     DQLLAPYTHYFNQTIHTVCNLPPSE"
     gene            complement(2739..4538)
                     /gene="dnaG"
                     /locus_tag="VFA14_03505"
     CDS             complement(2739..4538)
                     /gene="dnaG"
                     /locus_tag="VFA14_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HZS80482.1"
                     /translation="MIPQSFIQDLLNRIDIVDVVGRYVQLKKGGANFMGLCPFHNEKS
                     PSFTVSPAKQFYHCFGCGAHGTAISFLIEYSGLGFVEAVKELAQGVGMVVPDNDDRIP
                     PAQRAERQARSIALSDAMSAACDFYRQQLRVAPDAVDYLKRRGLTGEIAAKFGLGYAP
                     DEWESLRKVFPDYEASVLVEAGLVIDKGDEDGAQRMGRKRYDRFRHRVMFPIRNTKAQ
                     VIGFGGRVMDGGEPKYLNSPETPLFQKGSELYGLFEARQAIRDAGYVLVTEGYMDVVA
                     LAQWGFPQAVATLGTACTPVHVQKLLRQTDHVVFSFDGDAAGRRAARRALDACLAHAS
                     DDKIIKFLFLPAEHDPDSYVREMGAEAFERQVKEAMPLSQFLLREVIGDNDMDTPEGR
                     ARAQFDARPILQALPPSSLRLQIVRSLAQVTQSTVDEIETLFELTQPIARARRAPPRS
                     RRMAPVGLERQIARLLVAYPALVADLDDAALQAIGRQVPDQTRLLSQLVEAGRSLGEQ
                     ASFASMVEYLRDAGPDFEVLIAEVAVETGSEFDVVRQEMAGAVRQIKMRGVEDEMQQL
                     ASSGLEDDTARHRYRELTQQKEHLKRLSGAEFG"
     gene            complement(4548..5003)
                     /locus_tag="VFA14_03510"
     CDS             complement(4548..5003)
                     /locus_tag="VFA14_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013232899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HZS80483.1"
                     /translation="MGLKERITEDMKLAMRAKDAVRLGAIRLIMAVMKQKEVDERVEL
                     SDALVLAIIDKMVKQRKDSISQFEAGGRQDLADVEKAELAVLSAYMLAGLSDDEIRAE
                     VQAAVAATGAAGPQDMGKVMGALKPKLAGRADMTVVSALVKTALAAGLP"
     gene            complement(5174..5386)
                     /gene="rpsU"
                     /locus_tag="VFA14_03515"
     CDS             complement(5174..5386)
                     /gene="rpsU"
                     /locus_tag="VFA14_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011903537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HZS80484.1"
                     /translation="MTTIRLKENEPFEVAMRRFKRTIEKTGLLTELRAREFYEKPTAE
                     RKRKLAAAVKRHYKRIRSQQLPKKLY"
     gene            complement(5612..>6105)
                     /locus_tag="VFA14_03520"
     CDS             complement(5612..>6105)
                     /locus_tag="VFA14_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZS80485.1"
                     /translation="RGLSGPAILQISSFWQQGAALTLDLLPRIDVAKTLIEGKSTLRK
                     NLGKLLTQWLPSRLVDSLLEAHGFDGAARIADMPDRQLRQLGEALKRWTIKPNGSQGY
                     RKAEVTVGGIDTRELSQQSMMTNKVPGLHFIGEAVDVTGWLGGYNFQWAWASGVAAGR
                     SIE"
BASE COUNT         1221 a         1873 c         1588 g         1423 t
ORIGIN      
        1 aacaagccat ccgcaggctt aattgttatc caaaaaactg cgcagtcgct cagagcggga
       61 cgggtgacgt aacttgcgca atgctttcgc ttcgatctga cggatgcgtt cgcgcgtgac
      121 gtcgaattgc ttgccgactt cttccagtgt gtggtcggtc gacatttcaa taccaaagcg
      181 catgcgcagc actttggctt cgcgtggcgt cagcgagtcg aggatatcct tgaccacgcc
      241 gcgcatcgaa gcgtgcagcg cggcgtcgga cggagctaat gtattgttgt cttcgatgaa
      301 atcgcccaga tgtgaatcat catcatcgcc gatcggcgtc tccatggaga tcggttcctt
      361 ggcgatcttc atgattttgc ggatcttgtc ttccggcatt tccatcttga tagccagcgt
      421 cgccggatca ggttccgcac cggtttcctg cagtatctga cgcgagatgc ggttcatctt
      481 gttgatggtt tcgatcatat gcaccggaat acggatggtg cgcgcctgat cggcaatgga
      541 gcgcgtgatg gcctggcgaa tccaccatgt cgcataggtc gagaacttgt aaccgcgacg
      601 gtattcgaac ttgtccacgg ccttcatcag gccgatgttg ccctcctgga tcagatcgag
      661 gaattgcagg ccacggttgg tgtatttctt ggcgatcgag atcacgagac gcaggttggc
      721 ctcggtcatt tcccgcttgg ccatgcgcgc cttcttttcg ccggcgccca tttttttgtt
      781 gataccgcgc agatcgggca acggcagcac gacacgcgcc tgcaagtcga tcagtttctt
      841 ttgcagctcc ttgatcgcag gtatgttgcg gcccagcacc acgctgtagc tgtggttggc
      901 atcgacttcg ccatcgaccc agtccagatt gatttcgttg cctggaaaaa ccttgacgaa
      961 gtgcgcgcgc ggcatattgc atctgttgac ggcgacatca agaatctgac gctcgatctg
     1021 acgcacctgg tcgacctgcg agcgcagcgt gtcacacagt ttctcaacga ccttggcggt
     1081 aaagcggatg cccagcagtt cgttggaaat gacttcctgt gccttgacat aaggcttgga
     1141 gttgtagcct tctttttcaa aagccttgcg catgttgtcg aactggaccg agatgatcgt
     1201 gaacttgtcc agcgcgtcgc gtttgagttg ctcgagctgc tccgcggaaa aaccggccgc
     1261 accggaactg ctgctgtcgc tcccctcttc ttctgcctcg gcatcgtctt cttcatgctc
     1321 gtcgccatcg gaagcggcgg ctgtcacgac gacttcacct tcattcagat cgaccagacc
     1381 atcgacgatt tcatcgaccc tgatttcatc gttcctgatt ttttgggcaa cggcaaggat
     1441 ttcatcgatg gtggttggac aggccgagat cgcctggatc atgtctttca aaccgtcttc
     1501 gatgcgcttg gcgatttcga tttcgccttc gcgggtcaac agttccaccg aacccatttc
     1561 gcgcatgtac atgcgcacag ggtcggtagt gcggccaaaa tcggaatcga ccgtcgacaa
     1621 tgctgcttcg gcagcggctt cagcgtcgtc gtcgctggcg acgctgtccg acagcagcag
     1681 ggtttcagca tcgggcgcgt gctcgtacac agcgatgccc atgtcattga aagtaccgat
     1741 gatgccttca atcgcctcgg ggtcgacgat gttttccggc agatgatcgt tgatttccga
     1801 gaaagtcagg aaaccacgct ccttaccatg cttgatcagg gccttgagtt tttgacggcg
     1861 gcgttccagt tcttcttcgg tcgcttccgt atcggacgag aacgcatctt tcagtaaggc
     1921 tttttccttc gctttacggt ccttcgccct ggctttgtca acggccttga gttcggcacg
     1981 ctcaaccgca ttcaacgcag cgatttcgtc attctcaggc tggaattcct tcggcttgcg
     2041 gccacggcga cccggcacct tgacggatgg caggatatag ccggacgtat cgatggcggc
     2101 cagcgcggcg gcatcagtca tttggctcac gccagacacg atcatggcgt cgacacccct
     2161 ggcttcgctg gctgccttgg cgacaggcat ggcgatcttc gattcaggtt tcttgcttgt
     2221 cacagaagct ttcgattgca cagaagccgt ctttgggttg gctcgagata agttcttttt
     2281 cgttgatgct acagccgctc cggcactgga ttttttcgtc ggtgacgcta tcgctttggc
     2341 tatcgtcact actaccttgc cagtgcggct gacagcagtc gttcgtttcg gggccgatcg
     2401 cgctactgcg ggagattgtt tgactttctg tttaatagct gacgtgctgt tttttacgga
     2461 agtttttgcg gttttcttca ttgcatttgc catagatcaa ctgctggcac cttacactca
     2521 ttacttcaat caaaccatac acactgtatg caatcttccc ccctccgaat agacaggaac
     2581 tgcccgcgca acgttcctga gtctttctgt ttctgccttg tctatggatc gttcgccgcc
     2641 ggatttcgga tcatagcata tataacaatg cttttccccg catcgagcgg cgcttataca
     2701 acagctgaca caaggaagaa ctcaatccct gtttgctttc agccgaattc agcaccgctc
     2761 aggcgcttca aatgctcctt ttgctgcgtc agttcgcgat aacggtgccg cgccgtatca
     2821 tcctcgagtc cactgcttgc cagttgctgc atttcatctt ccacgccacg cattttgatc
     2881 tggcgtaccg cgccagccat ttcctgtctg acgacatcga attcggaccc ggtttcaacg
     2941 gcaacttcag cgatcagcac ctcaaaatcc ggtccggcgt cgcgtaaata ttcgaccatg
     3001 ctggcaaaac tggcttgctc gcccagactg cggccggctt cgactagctg cgacagcaag
     3061 cgcgtctgat ccggcacctg acgtccgatc gcctgtagcg ccgcatcgtc cagatccgct
     3121 accagcgccg gatacgccac cagcagacgc gcgatctgac gttccagtcc gaccggcgcc
     3181 atgcgcctag aacgtggcgg cgcccggcgc gcccgggcaa tcggctgcgt cagttcgaac
     3241 agtgtttcga tctcgtccac cgtactctgt gtcacctgcg ccaggctgcg cacgatctgc
     3301 aaacgcaaag atgacggcgg cagagcctgc aatatcggtc tggcatcaaa ctgcgcgcga
     3361 gcgcggcctt ccggcgtatc catatcgttg tcgccgatga cttccctgag caggaactgc
     3421 gacagcggca tcgcctcttt cacctgccgt tcaaacgctt cagcacccat ttcacgcaca
     3481 taactgtccg ggtcgtgttc cgccggcaga aacaggaact tgatgatctt gtcgtccgag
     3541 gcatgcgcca gacaggcatc cagtgcccgt cgcgcggcgc gacggccggc cgcatcaccg
     3601 tcgaagctga acacgacatg gtcggtctgg cgcaacaact tctgtacatg gactggcgta
     3661 catgccgttc ccaatgtcgc caccgcctgt ggaaaacccc attgcgccag cgccaccaca
     3721 tccatgtaac cttccgtcac cagcacgtaa cctgcatcgc gaatcgcctg acgcgcttcg
     3781 aacaggccat acagctcgct tcccttttga aatagcggcg tttccggaga gttcaggtat
     3841 ttcggctcgc cgccatccat gacgcggcca ccgaagccaa tgacctgggc tttggtatta
     3901 cgaatcggaa acatgacacg atggcggaac cggtcgtaac gcttgcgtcc catacgctgg
     3961 gcgccatcct catctccctt gtcgatcacc aggccggcct cgaccagtac cgacgcttca
     4021 taatccggga acaccttgcg caagctttcc cattcgtccg gcgcataacc cagaccgaac
     4081 ttcgcggcaa tttcaccggt cagcccgcgc ctcttcaaat agtcgaccgc atccggcgcc
     4141 acacgcaact gctggcgata aaaatcgcag gccgccgaca ttgcatccga cagcgcgatg
     4201 ctcctggcct gccgctcggc gcgctgcgct ggaggaatac ggtcatcgtt gtccggcacc
     4261 accatcccca cgccttgcgc aagctccttg accgcctcca caaaacccag cccggaatac
     4321 tcgatcagaa aactgatggc cgttccatgc gcgccacaac cgaagcagtg ataaaactgc
     4381 ttggccggac tgaccgtgaa gctcggcgat ttttcgttat gaaacggaca caggcccatg
     4441 aaattggcgc cgcccttttt cagttgcaca taacggccaa ccacatcgac gatatcgatg
     4501 cgattgagca agtcctgaat gaacgactgt ggaatcacgg acagacctta cggcaaaccc
     4561 gcagccaggg cagtcttgac cagagcagac accaccgtca tgtcggcgcg gccggccagc
     4621 ttcggcttta gcgcacccat gaccttgccc atgtcctgcg gaccggctgc gccggtagcg
     4681 gcgacagcag cctgcacttc cgccctgatt tcatcatcgg acaaaccggc aagcatatat
     4741 gccgacaata cggccagctc agccttctcg acatccgcca gatcctgacg accgccggct
     4801 tcgaactggc tgatggaatc cttgcgctgc ttgaccatct tgtcgatgat cgccagcacc
     4861 agtgcatcgc tcagctcgac acgctcatcg acctccttct gcttcatcac cgccatgatc
     4921 aaacgaatcg cgcccagtct gaccgcatcc ttggcgcgca tcgcaagctt catgtcttcg
     4981 gtgattcgct ctttcagacc cattgccttc tccatggata cgatgcattc agattcagga
     5041 agcgcagaaa aagatctcga tcaaacccgc gcagcaacct caaacatcct gcggaatacg
     5101 aaaacccgct gaggcctgta ccagagcggg ttcgatatga ccacgcgcca gacggcggcg
     5161 cggcggaaat tttctagtat aacttcttcg gcagttgctg gctgcggata cgcttgtagt
     5221 gacgcttcac tgcggcagcc agcttgcgct tgcgttccgc tgtcggcttt tcgtaaaact
     5281 cgcgcgcgcg caactcggtc aacaggccgg ttttttcgat agtgcgcttg aagcgacgca
     5341 tggcgacttc gaatggctcg ttttctttaa gacgaatagt ggtcatgtaa attcaaaccg
     5401 gaggttcagg atgagatatg taaaagccgg aattctagca gacgacgcca acataaacaa
     5461 gacttgaccc tctccttcac aggatttccg tgtttttatc gatcagtcaa aacgtcaaag
     5521 caactgtgct tgatgccacg actctttagt cttgacgcgg aattcaagca acgatgcgtg
     5581 aagctgtcag gctagccctg cccgtctagc gtcattcgat cgaccgtccc gccgccacgc
     5641 ccgaagccca tgcccactgg aagttatagc ctcccaacca gccggtgaca tccaccgcct
     5701 cgccaatgaa atgcaggccc ggcaccttat tcgtcatcat gctctgctgc gacagttcac
     5761 gcgtgtcgat gccgcccacg gtgacttccg ccttgcgata accctgggag ccgttgggct
     5821 tgatggtcca gcgcttgagc gcttcgccca gctggcgcag ttgtctgtct ggcatgtcgg
     5881 cgatgcgcgc cgcgccgtcg aaaccatgag cctcgagcaa gctatcgacc agacgggacg
     5941 gcagccattg cgtcagtagc ttgccgagat ttttcctgag agtggacttg ccttcgatca
     6001 aggttttcgc cacgtcgatt cgcggcagca gatcaagcgt cagcgccgcc ccctgctgcc
     6061 aaaagctgga aatctgcaga atcgccgggc cggacagacc gcgat
//
LOCUS       DASRDH010000153         5243 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_251875,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000153 DASRDH010000000
VERSION     DASRDH010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 5243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5243
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251875"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="VFA14_03525"
     CDS             complement(<1..401)
                     /locus_tag="VFA14_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HZS80486.1"
                     /translation="MTTDYLQKILTARVYDVAQETPLELARTLSQRMENRIYLKREDM
                     QSVFSFKLRGAYNKMVQLTPAQLERGVICASAGNHAQGVALSAAKLGCRAVIVMPTTT
                     PTVKIEAVKNRGGEVVLSGDSFTDAYNHALTL"
     gene            509..1000
                     /locus_tag="VFA14_03530"
     CDS             509..1000
                     /locus_tag="VFA14_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HZS80487.1"
                     /translation="MIENAILWLLKILAVPTVGLTSVFIISFVSATLLPLGSEPAVFA
                     VIKANMLMFWPVILVATVGNTLGGVVNYWMGYGARQTFAREHASRWFLWLERFGPKTM
                     LLAWLPGIGDPICTLAGWLKLPFWPSVIYMALGKFLRYISVTWLLLNVPDGFWRAVAD
                     CLA"
     gene            complement(992..1918)
                     /locus_tag="VFA14_03535"
     CDS             complement(992..1918)
                     /locus_tag="VFA14_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HZS80488.1"
                     /translation="MIPSPSRERLPVTSTPDTPFRLRPRQIVMLTVGLVFILATSYES
                     LRVFFLAYPEVKMALIGSSAAAGLTALGALGVLFTRRISERMQDVLLGYGGGIMLAAS
                     VFSLIMPAIQQAQVLGYEKTGAVLLTATGIMLGGLGVLILNYLVPHEHFIAPNPAVHS
                     MRASRIWMFIMAVTIHNFPEGLAIGVGFGGSDMGKAIALATGIGIQDIPEGLVVALAL
                     RTLGYSPLKSAGAGVLSGLVEPIGGVLGALATSASATALPWALASAGGAMLFVISHEL
                     IPESHRQGHETQATLGLLTGFVTMMMLDTLLG"
     gene            2240..>5243
                     /locus_tag="VFA14_03540"
     CDS             2240..>5243
                     /locus_tag="VFA14_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008332021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3683 domain-containing protein"
                     /protein_id="HZS80489.1"
                     /translation="MNAPAKIQVLLTDTPQAATPARLREIPYNYTSFSDREIVIRLLG
                     EEVWRLLDELRAKRQTGRSARMLYEVLGDIWVVRRNPYLQDDLLDNPKRRAALIDALN
                     HRLAEVDKRRIDISQGEVSDADAVRQSASVASLLRAAQKAVADFAEEFRQVRDLRRRA
                     NKILRRYTARDNIKFDGLSRVSHVTDATDWRVEYPFVVLTPDSENEVAGLVRACIELG
                     LTIIPRGGGTGYTGGAIPLTPMSAVINTEKLEQLGTVEMAMLPGLDRPYATIHSGAGV
                     VTRRVSDAAEKAGFVFAVDPTSAEASCIGGNVAMNAGGKKAVLWGTALDNLASWRMVD
                     PQGDWLEVTRLDHNLGKIHDVELARFRLEWSHPGEKGQKTEVFKTEILDIAGHKFRKE
                     GLGKDVTDKFLSGLPGIQKEGCDGLITSARWILHEMPKQTRTVCLEFFGQARDAIPSI
                     VEIKQYLDAETKKGGALLAGLEHLDERYLRAVGYATKSRRGVLPKMVLIGDIVGDDEN
                     AVAQAASEVIRIANNRVGEGFVAISLEARKKFWLDRSRTAAIAKHTNAFKINEDVVIP
                     LDRMGEYTDHIERINIELSIKNKLQLLGELHAFFVKGNLPLGKGDDAESEDIPAAEML
                     EDRVHQAGELIEQTQARWSWLLANLDKPLCVVKGEMAALGLEKLLPVFEQCAIDRTDA
                     SVFHVVQSRAVRVSWKQEVRAQLRRIFSGAAFMPVLEECDAIHERVLRGRVFIALHMH
                     AGDGNVHTNLPVNSDHYDMLQDAHEAVERIMMLARSLGGVISGEHGIGITKLEFLAEE
                     EINAFRDYKKRVDPDGHFNKGKLLDLPGMAADLRNAYTPSFSLMDHESLIMQQSDIGA
                     IASNVKDCLRCGKCKPVCSTHVPRANLLYSPRNKILATSLLNEAFLYEEQTRRGISIR
                     HWKEFEDVADHCTVCHKCVMPCPVDIDFGEVSMNMRNLLRKMGKRSFNPGTAAAMFFL
                     NATDPATINATRKVMTDWGFKAQRLGNDI"
BASE COUNT         1121 a         1398 c         1561 g         1163 t
ORIGIN      
        1 agcgtcagtg cgtggttgta agcatcggtg aaggagtcgc cggacaacac gacttcgccg
       61 ccgcggttct tgactgcttc gattttgacg gttggcgtcg tggttggcat cacgatcacg
      121 gcgcggcaac caagtttggc ggcggacaag gcaacgcctt gcgcatgatt gccggcggag
      181 gcgcaaatca cgccgcgctc gagctgcgcc ggtgtcagtt gcaccatctt gttatacgcg
      241 ccgcgcagtt tgaaactgaa cacgctttgc atatcctcgc gcttgagata aatgcggttt
      301 tccatccgtt gcgacagcgt gcgggcaagt tccaacggcg tttcctgggc aacgtcgtag
      361 acgcgggcag tgaggatttt ttgtaaataa tcggtggtca tggtcaggca agttcggggc
      421 gcggcggact ggttattata atgaagccgc cgtgcagact catgcagact ttcgccaact
      481 cctcacaaaa tgtatctttt cttcctgcat gattgaaaac gccattctct ggttactgaa
      541 aatacttgcc gtgccgacgg tcggattgac ttcggtcttc atcatcagtt ttgtttcggc
      601 gacattgttg ccgctgggtt cggagcctgc cgttttcgcg gtcatcaaag ccaatatgct
      661 gatgttctgg ccggtgattc tggtggcgac ggtgggcaat acgcttggcg gcgtggtgaa
      721 ttattggatg ggatatggcg ccaggcaaac tttcgccagg gaacacgcca gccgctggtt
      781 cctctggctt gagcgcttcg gtcccaagac catgctgctg gcttggctgc cgggcatcgg
      841 cgatccgatc tgcacgttag ctggctggct caaactaccg ttttggccgt cggtgatcta
      901 tatggcgctc ggtaaatttc tgcgctacat cagcgtgacc tggctgttgc tgaacgtgcc
      961 ggacggtttc tggcgcgctg tcgccgactg cttagcctaa caaggtatcg agcatcatca
     1021 tcgtcacaaa accagtcagc aggccaagtg tggcctgtgt ttcatgcccc tggcggtgtg
     1081 attcaggaat gagttcgtga ctgatcacga acagcatagc gccgccggcg cttgccagtg
     1141 cccatggcaa cgcagtcgcg gaggcgctgg tggctagcgc gccaagtacg ccgccgattg
     1201 gttcgaccag gcctgacaga acgccggcgc cggccgattt gagcggcgaa taacccaagg
     1261 tgcgcaatgc cagcgcgacg accagacctt ctggaatatc ctgaatgccg atgccggtcg
     1321 ccagtgcgat ggccttgccc atgtcgctgc cgccaaagcc cacgccaatg gccaggcctt
     1381 ccggaaagtt gtggatggtt accgccatga tgaacatcca aatacggctg gcgcgcatcg
     1441 aatgcactgc tggattgggg gcaatgaagt gttcgtgcgg caccagatag ttgaggatca
     1501 acacgcccaa cccgcccagc atgatgccgg tcgcagtaag cagcaccgcg ccggtttttt
     1561 cgtagccgag cacttgcgcc tgctgtatgg ctggcatgat cagcgaaaac acgctggccg
     1621 ccagcatgat gccgcctcca tagccgagta gcacgtcctg catgcgttcc gaaatacgcc
     1681 gcgtgaatag cacgccaagt gcgccgagcg ccgtcagtcc ggctgcggcg gaagaaccga
     1741 ttaacgccat cttgacttca ggataggcga ggaagaagac gcgcaggctt tcgtaagacg
     1801 tggccagaat gaagactaat ccgaccgtca gcatgacgat ctggcgtgga cgcagacgga
     1861 aaggggtgtc gggcgtcgat gtgaccggaa gtcgttcgcg tgatgggctt ggtatcatgg
     1921 cggtcatcct ttatggcgga taggaggatg gtggtcccgt caagccttgc actcagcgta
     1981 tgccgccagg gtgttggtga tattttccag aaattcggtc atcgataggg acaacatggc
     2041 atgctccgca ggatttgagt gcaatctata tgtatgcata ataggttcaa ataatagata
     2101 ttcctaaatt gattattttt atcgatgcca taatagaatt cttatattgc atcgcggaaa
     2161 ttcatgtaaa tgctgcgatt gttcagccaa accaacagta gaatgcatat tcagcaacag
     2221 actccagatt ctttcattca tgaacgcccc cgcaaaaatc caggtgttgc tcactgatac
     2281 accgcaagcg gccactccag cgcgcctgcg cgaaattcct tacaactaca cttcgttctc
     2341 cgaccgcgaa atcgtcattc gtttattggg tgaggaggtg tggcgtctgc tcgacgagtt
     2401 gcgcgccaag cgtcagactg gccgatctgc gcgcatgttg tacgaagtgt tgggtgacat
     2461 ctgggtggta cgccgcaatc cgtatttgca ggacgatttg ttggataatc ccaagcgccg
     2521 tgccgcattg atcgatgcgc tgaaccatcg tctggccgaa gtcgataagc gccgtatcga
     2581 catcagccag ggcgaagtga gcgacgctga tgctgtgcgc cagagtgcca gtgttgcatc
     2641 gctgttacgc gccgcgcaga aggcggttgc cgattttgct gaagaattca ggcaggttcg
     2701 cgatttacgt cgccgcgcca acaaaatcct gcggcgctac accgccaggg acaacatcaa
     2761 gttcgatggc ctgtcgcgcg tttcgcacgt gaccgacgct actgactggc gcgtcgaata
     2821 tccgttcgtc gtactcacgc ccgacagcga aaatgaagtg gccggtctgg tcagggcctg
     2881 tattgaactc ggtttgacga tcatcccacg tggcggcggc acgggttata ccggcggtgc
     2941 tattccgttg acaccgatgt cggctgtcat caatactgaa aagttggagc aattgggcac
     3001 cgtcgagatg gcgatgttgc ccggtctcga ccggccttat gcgacgattc attctggcgc
     3061 cggtgtagtc accagacgtg tttcggacgc ggccgaaaaa gctggttttg tgtttgcggt
     3121 cgatccgact tcggctgaag cgtcctgcat cggtggcaac gtcgccatga acgccggcgg
     3181 taagaaagcc gtgctgtggg gtactgcgct cgacaatctg gcaagctggc gcatggtcga
     3241 tccgcagggc gactggctgg aagtcacccg cctggatcac aacctgggca agattcacga
     3301 tgttgagctg gcgcgtttca ggcttgaatg gtcgcatccc ggcgagaagg ggcaaaagac
     3361 tgaagtcttc aaaactgaaa tcctcgacat cgccggccac aagtttcgca aagaaggttt
     3421 gggcaaggat gtcaccgaca aattcctgtc gggtttgccc ggcatccaga aggaaggttg
     3481 tgatgggctg atcacttcgg cccgttggat tttgcacgag atgccgaagc agacgcggac
     3541 cgtctgtctg gaatttttcg ggcaggcgcg cgatgcgatt ccgtcgatcg tcgaaatcaa
     3601 gcagtacctt gacgccgaaa ccaaaaaagg tggcgcgctt ctcgctggcc tagagcatct
     3661 ggacgaacgc tacctgcgcg cagtcggcta tgccaccaaa tccaggcgcg gtgtgttgcc
     3721 gaagatggta ttgatcggcg acatcgtcgg cgacgatgaa aatgccgtag cgcaagctgc
     3781 ttcggaagtg atccgtatag ctaataatcg cgtcggtgaa ggtttcgttg cgattagcct
     3841 ggaagcgcgc aagaaattct ggctcgaccg ttcgcgcacg gctgcgattg cgaaacacac
     3901 caatgccttc aagatcaacg aggatgtggt gattccattg gatcgcatgg gtgagtatac
     3961 cgaccacatt gagcgcatca acatcgaact gtcgatcaag aacaaactgc aattgttggg
     4021 cgaactgcac gcttttttcg tcaagggcaa tcttcctctg ggcaagggcg atgacgccga
     4081 aagcgaggat attcctgcgg ctgaaatgct ggaagaccgc gtccatcagg ccggggagct
     4141 aatcgaacaa acgcaggcgc gctggtcctg gctgctggcc aatcttgaca agccactgtg
     4201 tgtggtcaaa ggcgagatgg cggcgctggg gctggaaaag ctgctgccgg tattcgagca
     4261 atgcgcgatc gatcgaaccg acgccagtgt tttccacgtc gtgcagagcc gtgccgtgcg
     4321 tgtgtcgtgg aagcaggagg tgcgggccca gttgcgccgg atcttcagcg gcgctgcgtt
     4381 catgccggtc ctcgaagaat gtgatgcgat ccatgagcgc gtgctgcgcg gccgcgtctt
     4441 catcgcgctg cacatgcatg ccggcgacgg caacgtgcat accaatttgc cggtcaattc
     4501 cgatcactat gatatgctgc aagacgccca cgaagctgtg gagcgcatca tgatgctggc
     4561 gcgttcgctg gggggcgtga tttcaggcga acatggcatt ggcatcacca agctggagtt
     4621 tctggccgaa gaagaaatca atgcattccg tgactataaa aagcgtgtcg atccggacgg
     4681 tcacttcaac aaaggcaagc tactcgatct gcccggtatg gcagccgact tgcgcaatgc
     4741 ttatacgcct tccttcagcc tgatggatca tgagtcgttg atcatgcagc aaagcgacat
     4801 cggcgccatc gccagcaacg tcaaggactg cctgcgctgt ggcaagtgca aaccggtatg
     4861 ttcgactcac gtgccgcgcg ccaatctgct gtattcgccg cgcaacaaga ttctggcgac
     4921 atcgctgctg aatgaggctt tcctgtatga ggagcagacc cgccgtggta tctcgatcag
     4981 gcattggaaa gaatttgaag acgtggctga ccattgcacc gtctgccata aatgcgtgat
     5041 gccttgcccg gtcgacatcg acttcggcga ggtgtcgatg aacatgcgta atctgttgcg
     5101 caagatgggc aaaagatcct tcaatcctgg tacggcggcg gcgatgttct ttctcaacgc
     5161 taccgatccg gctaccatca acgccacgcg caaggtgatg accgattggg gtttcaaggc
     5221 gcagcgcctg ggcaatgaca ttc
//
LOCUS       DASRDH010000154         2467 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_252705,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000154 DASRDH010000000
VERSION     DASRDH010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2467
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252705"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="VFA14_03545"
     CDS             complement(<1..198)
                     /locus_tag="VFA14_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006029348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HZS80490.1"
                     /translation="MLPTSELIKNTITAGLACSHLEVEGDGRHFSAVIVSDVFSGKRL
                     IQRHRLVYAALGDRMREEIHAL"
     gene            complement(232..996)
                     /locus_tag="VFA14_03550"
     CDS             complement(232..996)
                     /locus_tag="VFA14_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008332476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZS80491.1"
                     /translation="MTGFQTLFYKEVLRFWKVATQTVTAPIMTAMLYLLIFGHVLQDR
                     VQVYPGVQYTGFLVPGLVMMSVLQNAFANSSSSLIQSKISGNLVFVLLTPLSHWEVFG
                     GYVLAAVVRGLVVGAGVFLVTAWFGHLSFAAPWWIAIFALLGAAILGTMGLLAGIWAE
                     KFDQLAAFQNFLIVPLTFLAGVFYSIHSLPPFWQTVSHFNPFFYMIDGFRYGFFGQSD
                     IDPVISLVIVAVFFIVLVTLVLRLLKSGYKLRSGTY"
     gene            complement(993..1904)
                     /locus_tag="VFA14_03555"
     CDS             complement(993..1904)
                     /locus_tag="VFA14_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008332478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZS80492.1"
                     /translation="MLALQINHVAKRYQSLQALGGVSLSIEEGEFFGLLGPNGAGKTT
                     LISIVAGLNRADAGSVAIHGHDVVSDYRAARARLGVVPQELVFDPFFSVRETLRMQSG
                     YFGLKNNDRWIDEVMDNLDLSSKADTNMRALSGGMKRRVLVAQALVHRPPVVVLDEPT
                     AGVDVELRRTLWKFIGRLNREGHTIVLTTHYLEEAQALCNRIAMLKLGKIVALDSTDA
                     LIKRISGSQLVLCLKQGGLPDSLRALVTHSEELFSGRKYTLRVNDCNDVEPILSLLRR
                     SGAVIDDMQLQQADLEDVFVQIMGVAQ"
     gene            2199..>2467
                     /locus_tag="VFA14_03560"
     CDS             2199..>2467
                     /locus_tag="VFA14_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS80493.1"
                     /translation="MASLPHLAWADLPLTVEDLITDKGKVKLELSMSYVNADRQGIST
                     ADPLTIQTGPASFVTLPTRVSESVGNSDIAAATLGLRYGFAAKAE"
BASE COUNT          607 a          736 c          661 g          463 t
ORIGIN      
        1 caatgcatga atctcctcac gcatacggtc gccgagcgcc gcatacacca gtcggtgacg
       61 ctggatcagg cgctttccgg aaaaaacatc ggaaacgatc acggcgctga agtgccgacc
      121 gtcaccttcg acttcgaggt gagaacaggc aaggccggca gtaatggtgt ttttgatgag
      181 ttcagatgtg ggtagcatga ttacagatca ctagattcca ttcatagcgc atcaatacgt
      241 gccgctgcgc agtttgtagc cgctcttgag caggcgtagc accagcgtca ccagcacgat
      301 gaaaaaaacg gcgacgataa caaggctgat cacgggatcg atatcggatt gtccgaagaa
      361 accgtaacgg aaaccgtcga tcatataaaa aaacgggttg aaatgcgaga ccgtttgcca
      421 gaacggcggc agcgaatgga tcgaataaaa cacgccggcc aggaaagtca gcggcacgat
      481 cagaaaattc tggaacgctg ccagctggtc gaatttttcg gcccagatac cggccagcag
      541 ccccatggtg ccgagaatcg ccgcgcccag caaggcaaag atcgcaatcc accacggcgc
      601 cgcgaaactc aggtgaccga accacgccgt caccaggaac acgccggcgc cgaccaccag
      661 cccgcgcacc actgccgcca gtacatagcc gccgaacact tcccagtgcg atagcggcgt
      721 caacagaacg aacaccagat tgccactgat cttggactga atcaatgacg acgagctgtt
      781 ggcgaaagca ttttgcagca cgctcatcat caccaggccg ggcaccagaa aaccggtata
      841 ttgcacgccc ggatacacct gcacgcggtc ttgcagcacg tgaccgaaga tcagcagata
      901 cagcatggcc gtcatgatcg gcgccgtcac ggtctgggtg gcgactttcc agaaacgcag
      961 cacttccttg taaaacaagg tctgaaaacc agtcattggg caactcccat gatctggacg
     1021 aagacatctt ccagatcggc ctgttgcaac tgcatgtcgt cgatcacagc gccgctccga
     1081 cgcagtagcg aaagaatcgg ttcgacatcg ttgcagtcgt tcacgcgcaa cgtgtacttc
     1141 cggcccgaga acaattcttc tgaatgcgtc accagcgcgc gcaggctgtc tggcaagccg
     1201 ccctgcttca aacacagcac cagttgcgaa cccgagatac gcttgatcag cgcatcggtg
     1261 gaatcgagtg cgacgatttt tcccagcttg agcatggcga tgcggttaca cagcgcctgc
     1321 gcctcttcca gataatgcgt agtcagcaca atggtatggc cttcacggtt gagacggccg
     1381 atgaacttcc acaaggtccg gcgcagctcg acatcgacac cggcagtcgg ctcatcgagc
     1441 acgacgaccg gcggcctgtg taccagcgcc tgcgccacca gcacgcgccg tttcataccg
     1501 ccggacaggg cgcgcatgtt ggtgtcggcc ttactgctca ggtcgaggtt gtccatcacc
     1561 tcatcgatcc acctgtcatt gttcttcaga ccgaaatagc cggactgcat gcgcaaggtt
     1621 tcgcgcacgc taaaaaaggg atcgaacacc agctcctgcg gcacgacgcc cagcctggcg
     1681 cgcgccgcgc ggtaatcgct gacgacatca tggccgtgga tggcgaccga gccggcatcg
     1741 gcgcgattga gtccggccac gatggaaatc aatgtggttt taccggcccc attggggccg
     1801 agcaaaccga aaaactcgcc ctcttctatc gagagcgaca caccgcccag cgcttgcaac
     1861 gactgatagc gttttgcgac atgattgatc tgcagggcaa gcattttctg gcgcgttttt
     1921 caggacagag tgagagaaca tgccgccagc cttttcttca aagcaacgct gtggcaaaac
     1981 cttgaattat aggggattcc tggttaacag gggttaatac tccagccctg accatggttg
     2041 tcatggaggt tgtaaccaat cagcggctca ggtttactct tgcagcccta acagggtatt
     2101 gacaactcaa agaaaggggc gataggatta ctacccaaaa tcatgttgag aattcaggac
     2161 aatcaagaga gtgcatgcgc gccatatctg gagtcctgtt ggcgtcactg ccccatctgg
     2221 catgggctga cctgccgctg acggtcgaag accttatcac cgacaaaggc aaagtcaaac
     2281 tcgagctgtc gatgtcttac gtcaatgctg accggcaagg catatccacc gctgatcccc
     2341 tcaccattca aaccggcccg gcctccttcg tcacgctgcc cacacgggtc agcgaaagcg
     2401 tcggcaacag tgacatcgcc gcagccactc tgggcctgcg ctacggcttc gccgccaagg
     2461 ccgaaat
//
LOCUS       DASRDH010000155         3737 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1097003,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000155 DASRDH010000000
VERSION     DASRDH010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 3737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3737
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1097003"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(215..1561)
                     /locus_tag="VFA14_03565"
     CDS             complement(215..1561)
                     /locus_tag="VFA14_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS80494.1"
                     /translation="MLASAALGASIAAPTHATAEYALQSGVSWYRFDNLFLFRNDSDN
                     KLHTSDSAWSADLRGALFLPLPTGRSNLQLTASASQMHYSKFSAYTNSTGHGPYSLNR
                     SKKQFDAMYTWAYGDLLHGHLRHRIDDRLYNYFGGEITPQTDPNGLPPRNLSNIEPEF
                     PHIREDRVEIAWRASKRFDMPLIWAQQTLSYMVPGRAEIHNMSSHALLAAIRYTSGRK
                     STLSAGIRRNKARFPDRNDLDIARFDSGYTDIEWFANSAWRYTENTIVNAHLGAVTRR
                     FHTLPERDRRFPWTELGFDWHFSARTTFYGNIWNRPQTRDEAHGHLYDVMTRGIQGHA
                     IWQATPKTRVSLLGALRSQKSPNLAIDQFSTPSDNHDQWLRLGLRYDIDLTRRLAIRI
                     NAMHEKNVSGSDDSIRYRHSAAQLSLNYTFDNTTGPFDVYNPQGYGHARRQIENLR"
     gene            complement(1587..>3737)
                     /locus_tag="VFA14_03570"
     CDS             complement(1587..>3737)
                     /locus_tag="VFA14_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HZS80495.1"
                     /translation="DGHFYRTGPDRQPHTADDTRVRLYGINLSFAGNFPDDAVRLALR
                     LRKLGFNAVRLHHMDSSPGIQDNPPRSLLTPGPYPSFNQLALSRLRNFITALGQQGIY
                     VNLNLHVGYRFRPAVDQLPPLDEKDEATAPGAPVHVYYPRMVALQEEYARQLIRSLGL
                     RNDPALAMVEINNESSLLAAWQHRKWRAAVPKAYEAELRGQWQQWLIRRYGSTARACA
                     VWKTCATPDSVVELLTPPADASGPIPWLDRLSSKLDALARKALGSCDPGVPDTSGEGL
                     RLRDFLMFLADTDRAYFNRLRQVVQEETDALVPVTGTQMHYGGVLNFDSQAQMDYVDE
                     HVYIDHPNFPGVAWDRNDWRIRNSAVSDGELHSLLALALRRDRRKPFVVSEYNQPFPN
                     QQGSEIQPLMAAFAAAQDWDGLFFFDYINGDNWAATPSSFTLSGDWGKYAVTGQSAML
                     FRNGLVAPLPASMDIPLDPEARRAIAAARESDAFETHLSVRYGITPEQAMLMRLYIDL
                     NGTSRLNKQTPPASEPLRSPNGEFEFDARQRTIQIRTPQARGFFGFLMQRRVGDDNVA
                     IELPDTGRGFIAVLLTALDNRPLASSRQILVSASGAITGNQPGSIPPRPKKLIHYKND
                     NQWWTLEPDADMTDKPSGPRDAAGPVWMERSEARISLRTKAKRITVYPLDGSGKRLDA
                     LDSSCVSLDKGMASIHIQADASQASPWYEILAED"
BASE COUNT          694 a          998 c         1216 g          829 t
ORIGIN      
        1 tggttgagac cggctacggg ctggtgcctg cctggcaatc ctttggctcg actacaaaga
       61 tattcgttac tgattaactc ggctgagatg ctgaaccaat gtcacggcag ctattggtgg
      121 ctaccgtcgg ccagcggaac agattgatgg tagaagtgat ggtaggtatt ggaagtcgga
      181 ttaggatctc cttacactat tgagacttaa acggtcatcg cagattctcg atttgccggc
      241 gggcatggcc atagccttgc ggattgtaca catcgaacgg cccggtggtg ttgtcgaagg
      301 tgtagttcag actgagctgt gcggcgctgt ggcgatacct gatgctgtcg tccgatccgg
      361 agacgttctt ttcgtgcatg gcgttgatac ggatagccag tcgccgcgtg agatcgatat
      421 cgtagcgtaa gcccaggcgc agccattggt catgattatc gcttggcgtg ctgaactgat
      481 cgatagcgag gtttggcgat ttttgcgacc tcagcgcgcc cagcagggag acgcgggttt
      541 tcggcgtggc ctgccagatg gcgtggcctt gaatacctct ggtcattaca tcgtacaagt
      601 ggccgtgggc ttcatccctg gtttgcggac ggttccagat attgccgtag aacgtcgtcc
      661 tggctgaaaa gtgccagtcg aagccgagtt cggtccacgg gaagcgtctg tcgcgctccg
      721 gcagggtgtg gaagcggcgt gtgacggctc ccaggtgcgc gttgacaatg gtgttttcgg
      781 tgtagcgcca tgcgctattg gcaaaccatt cgatatcggt gtagccggag tcgaatctgg
      841 cgatgtccag gtcgttgcga tcgggaaagc gcgctttgtt gcggcgtatg ccggccgata
      901 aagtcgattt cctgccggag gtataccgta tcgccgccag tagggcatgg ctgctcatgt
      961 tgtgtatctc agcccggccc gggaccatgt aactcagcgt ttgctgcgcc cagattaacg
     1021 gcatgtcgaa gcgtttgctc gcccgccagg cgatttcgac acggtcttcg cgtatatgtg
     1081 ggaactccgg ttcgatgttg gagagattgc ggggcggcag gccattgggg tctgtttgcg
     1141 gtgtgatctc gccaccgaaa taattgtata gccggtcgtc gatacgatgg cgtagatgac
     1201 cgtgcaacag gtcgccgtat gcccaggtat acatagcatc gaactgtttt ttgctccggt
     1261 tcaatgagta agggccatga ccggttgagt tggtgtaggc actgaatttg ctgtaatgca
     1321 tctgcgatgc cgacgccgtc aattgcagat tgctgcgccc ggtcggcagt ggcagaaaca
     1381 gcgcgccgcg cagatcagcg gaccaggccg agtccgatgt atgcagtttg ttgtcactat
     1441 cgttccgaaa cagaaaaaga ttgtcgaagc gataccagct cacgccgctt tgcagtgcgt
     1501 attcagcggt ggcgtgggtt ggcgcggcga tcgatgctcc cagagcggcg ctcgccagca
     1561 tggcatgaaa tcgggatggc agcgcgtcaa tcctcggcaa ggatttcgta ccatggactg
     1621 gcctgggaag catcggcctg aatatggata ctggccatgc ctttgtccag gctgacacag
     1681 ctgctgtcga gtgcgtccaa gcgtttaccg ctgccgtcca atggatagac ggtaatccgt
     1741 ttggctttgg tgcgtaggct gatgcgggct tcgctgcgct ccatccagac ggggcctgcg
     1801 gcatcacgcg gaccggaggg tttgtcggtc atgtccgcat ccggctccag cgtccaccat
     1861 tgattgtcgt ttttgtagtg gatgagtttt ttcggacgtg gcggtatcga acccggttga
     1921 ttgccggtga tggcgcccga tgcggaaacc agaatctgcc gcgacgatgc cagtgggcga
     1981 ttgtccagcg ctgtcagcag cacggcaatg aagccgcgcc ccgtgtcggg cagctcgatc
     2041 gcgacattgt cgtcgccgac gcggcgctgc atcagaaaac caaagaaacc gcgcgcttgc
     2101 ggtgtgcgaa tctggatcgt gcgctgccgc gcatcaaatt cgaattcgcc attcggtgaa
     2161 cgcagcggct ccgatgctgg tggggtttgc ttgttcagcc tgctggtgcc gttcaggtcg
     2221 atgtagaggc gcatcagcat tgcctgttcc ggggtgatgc cgtaacgtac cgacaggtgt
     2281 gtttcgaaag cgtcggattc gcgcgcggcg gctatcgcgc gccgcgcctc ggggtcaagc
     2341 ggaatgtcca tcgatgccgg cagcggcgcc accagaccgt tacggaacag catggcactc
     2401 tggccggtca cggcgtattt tccccaatcg ccgctgagtg tgaatgagga tggcgtcgcc
     2461 gcccagttat cgccgtttat gtaatcgaag aaaaatagtc catcccaatc ctgggccgcg
     2521 gcaaaagctg ccatcaatgg ctggatctca ctgccttgct ggttggggaa gggctggttg
     2581 tattcgctga ccacgaaagg tttgcgtctg tcgcggcgca atgccagcgc cagcagcgag
     2641 tgcagctcac cgtcgctgac ggcgctgttg cggatgcgcc agtcgttgcg gtcccaggcg
     2701 acgccgggaa agttgggatg gtcgatgtaa acatgctcat cgacataatc catctgcgcc
     2761 tgagagtcga aattgaggac gccgccgtag tgcatttgcg tgccggtgac cggcaccagc
     2821 gcatcggttt cctcttgcac gacttggcgc aggcggttga agtaagcgcg gtcggtatcg
     2881 gccaggaaca tcagaaaatc gcgcaggcgc aagccctcgc cggacgtatc gggtacgccg
     2941 ggatcgcagg aacccagcgc ttttctggct agcgcatcga gtttgctgct gaggcgatcg
     3001 agccatggaa tgggtccgga tgcatcggcc ggcggtgtca gcaattcgac gacggaatcc
     3061 ggtgtggcgc aggttttcca tactgcgcag gcccgggctg ttgaaccgta gcgtctgatc
     3121 agccattgct gccattgccc gcgcagttcc gcttcatacg ctttgggtac ggcggcacgc
     3181 cacttgcggt gctgccaggc cgccagcagt gaagactcat tattgatctc caccatcgcc
     3241 agcgcgggat cgttgcgcaa gccgaggctg cggatgagct ggcgtgcgta ttcctcctgt
     3301 agcgctacca tacgtgggta atagacatgc acaggcgcgc cgggcgcagt cgcttcgtcc
     3361 ttctcgtcca gcggcggcaa ctgatccacg gccgggcgga aacgatagcc gacatgcagg
     3421 ttgaggttga catagatgcc ttgctgcccg agtgccgtga tgaaattgcg caggcggctg
     3481 agcgcaagct ggttgaagct gggatagggg ccgggcgtga gcaggctgcg cggcggattg
     3541 tcctgtatgc ccggactgct gtccatatga tgcagacgca cggcattgaa gccgagcttg
     3601 cgcaggcgca gcgccagccg gacggcatcg tcaggaaaat tgcctgcgaa gctcaggttg
     3661 atgccgtaca ggcgcacgcg cgtgtcatcg gcggtatgcg gctggcggtc cggtccggta
     3721 cgatagaaat gaccatc
//
LOCUS       DASRDH010000156         2143 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_939547,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000156 DASRDH010000000
VERSION     DASRDH010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_939547"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..910
                     /gene="acnA"
                     /locus_tag="VFA14_03575"
     CDS             <1..910
                     /gene="acnA"
                     /locus_tag="VFA14_03575"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141279.1"
                     /note="catalyzes the conversion of citrate to isocitrate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HZS80496.1"
                     /translation="DKLLKFAMNAKTFKENYAGVKGMPGKLWEAIKGVAKGEVYNWPA
                     STYIAEPPFFENFTEVPVAATPCIIGARALSVFGDSITTDHISPAGSIKDSSPAGKWL
                     QENGVLKADFNSYGSRRGNHEVMMRGTFANVRIKNLMVPGVEGGVTIHQPSGEQMAIY
                     DAAMRYVKDGVPTMVFGGEEYGTGSSRDWAAKGTQLLGVKAVITRSFERIHRSNLVGM
                     GVLPLQFIGNDSVQSLGITGEETFDLKGLESGIKPQQEAMLVIHRKNGETREVKLLLR
                     IDTPIEVEYYRHGGILPFVLRQLLAA"
     gene            1201..>2143
                     /locus_tag="VFA14_03580"
     CDS             1201..>2143
                     /locus_tag="VFA14_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2863 family protein"
                     /protein_id="HZS80497.1"
                     /translation="MRRSAKRSIPRLSAESQRLVNVSQAIASATSRLEESAWKRSLDV
                     SLNKQLKNHHQSMIDAALDYLFGADPSAYDVLVEAVEANSEACAFEYDGQLYSVQLLA
                     LPVLAWTRFSIASGNLSTDVCNGLFAHLHAHILASDVHLAVAPTLYAIEQLPRHHADV
                     FALTQRMGQAALEQSQPQLALEQAPTAPFLADTRYLIVAVAVPSGAPIFRWQMDEQIP
                     VAHEKNAALQAWMTQATPLFTRFLPGCNVELLLPQPYYFACREADKRIRPAAIRAADY
                     YLTQSLGLVSSELHANIGSFCENISGQVDEYRIGFSLG"
BASE COUNT          463 a          576 c          604 g          500 t
ORIGIN      
        1 cgacaagcta ttgaagtttg cgatgaacgc gaagacgttc aaagaaaatt acgccggcgt
       61 caaaggcatg ccaggcaagc tgtgggaagc gatcaagggc gtcgccaagg gcgaagttta
      121 taactggccg gcatcgactt acatcgctga accaccattc ttcgaaaatt ttaccgaagt
      181 accggtggca gcaacaccct gcatcatcgg cgcgcgcgcg ctgagtgtgt tcggtgattc
      241 gattaccacc gaccatatct ccccggctgg ttcgatcaag gactccagcc cagccggcaa
      301 gtggttgcag gaaaatggtg tactcaaggc cgacttcaat tcttatggct cgcgccgcgg
      361 taaccacgaa gtcatgatgc gtggcacatt cgccaacgta cgcatcaaga acctgatggt
      421 gccgggcgtt gaaggcggcg tgacgattca ccagccaagc ggcgagcaaa tggcgatcta
      481 tgatgccgcc atgaggtacg tgaaagacgg cgtgccaact atggtgtttg gcggcgaaga
      541 gtacggtacc ggatcctcgc gcgactgggc tgccaagggt acccagttgc tgggcgtgaa
      601 agccgtgatc acacgctcct tcgagcgtat tcaccgctcg aacctggtcg gcatgggggt
      661 gttgccgctg caattcatcg gcaacgacag tgtgcagtcg ctgggtatca cgggtgaaga
      721 aacattcgac ctgaagggcc tggaaagcgg aatcaagcca cagcaggagg cgatgctggt
      781 gatccatcgc aagaatggtg aaaccaggga agtcaaattg ttgctgcgca tcgatacacc
      841 gatcgaagtg gaatactaca gacacggtgg catcctgccc ttcgtactgc gccagttgct
      901 ggcagcttga tactccgcct gaccctctcg ctccgtggag tacacgagca gtgccggttc
      961 gaagtaattc gaccggtatt tcttgttggc caaacatgct tctctagcgg tctttcgccc
     1021 tggttgtcat agtagaatcc attcattggg tttacctgtt gcaaaacagg cccctgtgcg
     1081 gcaattttta tcagatttga gctgtgtgcg gtgacagttg cccgcatagt gctgagaagg
     1141 gcagattctg gtaacagctt ttttctgtct tatactattt gcttgtcatt attttttatc
     1201 atgcgccgct ccgccaaacg ctccatcccc aggttgtccg ctgaaagtca gcgattggtc
     1261 aatgtttcac aggcgattgc cagtgccacc agccgcctag aagaaagcgc ctggaagcgc
     1321 tctctcgatg tctcgttgaa caagcaactg aaaaatcatc accagagcat gattgacgct
     1381 gcgttggact atctgttcgg cgctgatcca tccgcttatg atgtgctggt ggaggctgtg
     1441 gaggccaata gtgaagcctg tgcattcgaa tatgacgggc agctttacag tgtgcagttg
     1501 ctggccttgc cggtgttggc ctggacccgt ttcagcatcg cctccggcaa cttgtcaact
     1561 gacgtgtgca atggtttgtt tgcccatctg cacgcccata tcctggccag tgacgtccac
     1621 ctggcagtgg cgccaacgct gtatgccatc gagcagttgc cacgccatca cgctgatgtc
     1681 ttcgcactga cccagcgcat gggccaggcg gctcttgagc aaagccagcc gcagctcgcg
     1741 ctggagcagg cgccgacagc gccctttttg gccgacacgc gctacttgat tgttgccgtg
     1801 gcagtgccgt caggcgcgcc gatatttcgt tggcaaatgg atgaacaaat accggtggcc
     1861 catgaaaaaa acgctgcgct gcaagcctgg atgacccagg cgacaccttt gttcacgcgt
     1921 tttctgccag gatgtaatgt tgaactgttg ttgccgcagc cctactattt cgcttgccgc
     1981 gaagccgata aacgtattcg tcctgcagca atccgtgctg ccgactacta cctgactcag
     2041 tcgctgggcc tggtatcgtc ggagctgcat gccaatatcg gcagtttctg tgaaaacatc
     2101 agcggtcagg tcgatgagta tcgcattggt tttagcttgg gtg
//
LOCUS       DASRDH010000157         4026 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_577903,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000157 DASRDH010000000
VERSION     DASRDH010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 4026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4026
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_577903"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..689
                     /locus_tag="VFA14_03585"
     CDS             <1..689
                     /locus_tag="VFA14_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZS80498.1"
                     /translation="EFIEKVAVMGERRVKLPVLTALKKHPRSFLLIIALRMAEMFTMY
                     IVTAFALSYSTANLGMSSDLFLNIGLLVGAISCLTIPVFAILADRIGQKRIYVTGAVI
                     GLLSAVPFFMAMEARSVFWIVIFSMLLVNIAHDMVVSVQQPLFTEFFGTEYRYSGAGV
                     GYQFASVVCGGFTPFIATALLGINGSWHAVAAYLAGGCLVSVVVASRMKMGAEAAHHR
                     PVSSDVIVND"
     gene            747..872
                     /locus_tag="VFA14_03590"
                     /pseudo
     CDS             747..872
                     /locus_tag="VFA14_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011301143.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
     gene            complement(912..1694)
                     /locus_tag="VFA14_03595"
     CDS             complement(912..1694)
                     /locus_tag="VFA14_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007882946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HZS80499.1"
                     /translation="MKNNELLEVQVARRDLIDGLIKGIDVITAFNDDNARLSASELAE
                     KVHLSRSAARRYLLTLVHIGMAATDGRIFWLTPKVLNLGRSYLDSARLPRSIVPFLQR
                     LTVQLQESTNYSVLDGDEVVYVSRVNAPQLLTTSFEPGTRLPAFTSTAGRVLLAALTD
                     EQLHTYLERVELMPYTHFTVTDKDQLLRELMTIREQGFSVTENQYEIGLRGISVPLKS
                     RRGMLIGALSVSMMTADKPKAEATARCVDALQATANTLMMWL"
     gene            1840..2718
                     /locus_tag="VFA14_03600"
     CDS             1840..2718
                     /locus_tag="VFA14_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020653015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HZS80500.1"
                     /translation="MTAQECNPEPNNPLGINGIEFIEYATAQPLAFGALLEQMGFAPT
                     ARHRSREVTLYRQGTMNVIVNADPKALPQSDIDPQSTVISAIALRVRDANAAYLHALE
                     MGAWAIQTRAGVMELNIPGVHGAGDSILYFVDRFSDFSIYDVDFKPIPNAPASPPALA
                     GMHFFGVVQAIGRDRSPEWIDFYQQLMDFHPLPQGRYFGVLPKGTLLESPCHNFYLQL
                     VEPPEGAVGLQWDEQLIRVGLGAPDVLAAVQALKQRGIVFIDREFAQISEKGALTQLY
                     KSGVSFELVVSHLEPK"
     gene            2715..3602
                     /locus_tag="VFA14_03605"
     CDS             2715..3602
                     /locus_tag="VFA14_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HZS80501.1"
                     /translation="MNLSNFGMDSITLAGPLESKLRASREAGFSQIMLWTKDLAAYPG
                     GVAQAVRAIRESGVRVTGIQVMRDFEGLSGSLHEYKLDIAKNLLQVCKAVGAPLLMVC
                     SSTSSQASSELPVIARHLAKLATLAVPLGVRIGYEALSWGRHVNQYTQSWEAVELADH
                     SNLGVVLDSFHMLANQADLDGLEDIPSHKIALVQLSDFMWRDIRSAEERLETARHLRV
                     FPGEGVHSEELSDLLRRLDRRGYRGDYSFEVFNDDYLQLSPEIVAQRAHGSAKWVTDQ
                     VLRRSLQVRQDTAGQPSMA"
     gene            complement(3675..>4026)
                     /locus_tag="VFA14_03610"
     CDS             complement(3675..>4026)
                     /locus_tag="VFA14_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HZS80502.1"
                     /translation="RSGKTPTSLYLAMQYGIKAANYPLIPEDFERNKLPGGLAAFRKK
                     IFGLTIAPERLSKIRHERRPGSKYAAIENCRYEVNEAEKMMRREGIRWMSSTAKSIEE
                     IAATILQEIRQETR"
BASE COUNT          815 a         1046 c         1164 g         1001 t
ORIGIN      
        1 aggagtttat tgaaaaagtc gccgtcatgg gtgagcgcag ggtcaagctt cccgttctta
       61 ccgcattgaa gaaacacccc aggagctttt tgctgatcat tgcgctgcgc atggcggaaa
      121 tgtttacgat gtatatcgtt actgcatttg ccttgtcgta ttcgacagcc aatctgggca
      181 tgtcgagtga tctgtttctc aatatcggtt tgctggtggg ggcgatcagc tgcctgacaa
      241 ttcctgtctt tgccatattg gctgaccgta ttggtcaaaa gcgtatttat gtcaccggcg
      301 cagtcatcgg cttgctgtct gccgtaccgt tttttatggc gatggaagcg cgttcggtgt
      361 tctggatcgt catcttctcg atgttactgg tgaatatcgc gcatgatatg gtcgtcagtg
      421 tgcagcaacc tttattcacg gaatttttcg gtactgaata tcgttatagc ggcgccggtg
      481 tcggttatca atttgccagt gtggtatgcg gcgggttcac gccattcatc gccacggccc
      541 tgctcggtat caatggctcc tggcatgcag tagcggctta tcttgccggt ggttgcctgg
      601 tatcggtcgt ggtggcttcc agaatgaaaa tgggagcgga agctgcgcat catcggcctg
      661 tatcatcaga tgtgattgtc aatgattaaa acggcagcag ggatttttca tgatcgacgg
      721 taaaaccaga ctgaccgtcc acttccaggg gttgcaggat tcaggtcggc gccgacatgc
      781 tgtttgaaat gattccggcg tatctggaat ttttcggttt tggcatggcg acaccggaac
      841 aactgcgccg ggtcgcgcgg atcgtctatt gaacgatgat gtgccgggcc aatgggcttg
      901 gggtggtttg atcaaagcca catcatcaaa gtattggcgg tcgcctgcaa agcgtcgacg
      961 caacgcgccg tcgcttctgc tttgggcttg tccgcagtca tcatggaaac gctcagagcg
     1021 ccgatcagca tgccgcggcg acttttgagc ggcaccgaaa tgccgcgcag acctatttca
     1081 tactggtttt ccgtgacgct gaaaccctgc tcccgtatcg tcatcaattc acgcagcaac
     1141 tgatctttgt cagtcaccgt aaagtgcgtg taaggcatca gctcgacacg ctccagatag
     1201 gtgtgtagct gctcatccgt caatgcagcc agcaacaccc gaccggcagt cgaagtgaag
     1261 gccggtaaac gcgtgccggg ttcaaaactg gtggtcagca gctgtggcgc attgacgcgg
     1321 ctgacataga ccacttcatc accgtcgagt acggaataat tggtcgattc ctgcaactgc
     1381 acggtcagtc tttgcaggaa gggaacaatc gatcttggca aacgcgcaga gtccagatag
     1441 gagcggccca ggttgagtac cttgggtgtg agccaaaaaa tgcggccatc agtggccgcc
     1501 atcccgatat gtaccagcgt caacagatag cggcgcgcag cgctgcggct gagatgcact
     1561 ttttcggcca gttcgctcgc gctcagccgc gcattgtcgt cgttgaaggc agtgatgaca
     1621 tcgatgccct tgatcaagcc atcgatcaaa tcgcgcctgg cgacttgcac ttccagcagt
     1681 tcgttattct tcactgtctc tggattccaa atagattcag catcgtgcga taatcgctcc
     1741 aattgggcga ttatcacagc attcgatgcc ttcagcacta tttaatcctg gattgcccat
     1801 cttatactga accccccatt tctctacctg gaactcatga tgactgcgca ggaatgcaat
     1861 cctgaaccga ataatccact cggcatcaat ggtatcgaat tcattgaata tgctactgcg
     1921 cagccgttgg cgtttggcgc gctgcttgaa caaatgggat tcgctcctac cgcccgtcat
     1981 cgttcgcggg aagtgacgct gtatcgccag gggacgatga atgtcatcgt caatgccgac
     2041 cccaaggccc tgccgcagtc cgacatcgat ccgcaatcga cggtgatcag cgccatcgcc
     2101 ctgcgcgtgc gtgatgccaa tgcggcgtat ctgcacgctc tggaaatggg cgcatgggcg
     2161 attcagaccc gcgccggtgt gatggaactg aatatccccg gcgtacatgg cgccggtgat
     2221 tcgattcttt attttgtcga tcgctttagt gacttttcca tttacgacgt cgatttcaag
     2281 ccgattccga atgcacccgc cagtccgccg gcattggcgg ggatgcattt ctttggcgtg
     2341 gtgcaagcca ttggtcgtga ccgctctccg gaatggattg atttttatca gcaactcatg
     2401 gatttccatc ccttgccgca aggccgttat ttcggcgtgc tacccaaagg caccttgctc
     2461 gaaagcccat gtcacaattt ttatctgcaa ctggtggagc cacccgaggg tgctgtcggt
     2521 ctgcaatggg atgagcaatt gatccgcgtt ggcctgggcg cgccggatgt gctggctgcg
     2581 gtgcaggcgc tcaagcagcg tggtatcgtt tttatcgatc gcgagtttgc acagatcagt
     2641 gaaaaaggcg cgctgacgca actttataaa agcggcgtca gctttgaact ggtcgtcagt
     2701 cacctggaac cgaaatgaat ctgagcaatt ttggtatgga cagcattacc ctggccgggc
     2761 cactggagtc caagttgcgc gcctccaggg aggcgggctt ttcgcagatc atgctgtgga
     2821 ccaaggatct ggccgcttat cccggcggtg tggcgcaggc agtccgcgcg atcagggaaa
     2881 gcggtgtgcg cgtgaccggt attcaggtga tgcgggattt tgaaggtctg tccggcagtt
     2941 tgcacgaata caagctcgat atcgccaaaa atctgctgca agtgtgcaag gctgtcggcg
     3001 ctcctctgct gatggtgtgt tccagtactt ccagccaagc cagcagcgaa ctgccggtga
     3061 tcgcgcgcca tctggcgaag ctggcgacgc tagcggtgcc gctgggcgtg cgtatcggct
     3121 atgaagcgtt atcatggggt cgccatgtca accagtacac ccaatcctgg gaagcggtgg
     3181 agttggccga tcattcgaat ctgggcgtgg ttctcgactc ttttcatatg ctggccaatc
     3241 aggccgatct cgatggcttg gaggatattc cgagtcataa aattgccctg gtacagcttt
     3301 ctgatttcat gtggcgtgat attcgcagcg ccgaggaaag gctggaaacg gcgcgtcatt
     3361 tgcgcgtctt tccgggggaa ggcgtgcatt cggaggagtt gtcggatttg ctgcgcagac
     3421 tggaccgacg cggttaccga ggcgactaca gtttcgaagt attcaatgac gattatctgc
     3481 aattatcgcc ggagatcgtt gcgcagcgcg cccacggttc tgccaaatgg gtgaccgatc
     3541 aggttttgcg gcgcagcctg caggtgcgtc aggatacggc tggacagcca agcatggcat
     3601 gaacaggaag cttctatcgc atcgtttggg atgaaattac ccaggtacgc gggagaagca
     3661 ggccatatcc ccggtcagcg cgtctcctgc ctgatttcct gcaggatggt ggcggcgatt
     3721 tcttcaatcg acttggcggt ggatgacatc cagcggatgc cttcgcggcg catcatcttt
     3781 tcggcctcgt tgacctcgta gcggcagttt tcgatggcgg catatttgct gccgggacga
     3841 cgctcgtgac gtattttcga caggcgttcg ggcgcaatcg tcaagccgaa aatctttttc
     3901 ctgaaagcag ccaggccgcc cggcaattta ttgcgctcga aatcttccgg gatcagcggg
     3961 tagttggctg ccttgatgcc gtattgcatg gccagataga ggctggtcgg ggttttgccg
     4021 gagcgc
//
LOCUS       DASRDH010000158         3005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1479830,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000158 DASRDH010000000
VERSION     DASRDH010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 3005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3005
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1479830"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..493)
                     /locus_tag="VFA14_03615"
     CDS             complement(<1..493)
                     /locus_tag="VFA14_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008117329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZS80503.1"
                     /translation="MGKFFIDRPVFAWVLAIFIMLAGALAITSLPVAQYPSIAPPTIV
                     ISAAYPGSTAQVLDDAVTSLIEQEMNGADGLQYIESQSQANGQVQITVTFSPETNPDL
                     AAVDVQNRLKRVEARLPAAVTQQGVQVTKSRSNFLLFISLSSTDGKLDPMALGDYLSR
                     NVLN"
     gene            complement(505..1671)
                     /locus_tag="VFA14_03620"
     CDS             complement(505..1671)
                     /locus_tag="VFA14_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HZS80504.1"
                     /translation="MSSVSRFTHLTLIVAVLAAFAGCGQKQSTQPGANMPPGEASVVT
                     IAPERITVTNELPGRLEATRIAEVRARVAGIILKRSFREGSEVKAGDVLYHIDPATFK
                     ASYDSAQATLTQATLKVQRYKPLAEVNAVSKQEYDDAVAAQKQAEAAMQIAGLNLGYT
                     TVTSPISGRIGRALVTEGALVGQGEATQLAVVQQLDPIYVNLTQSSTDLLKLQQAMAS
                     GQLKTVGKDKARVSLVTEDGRPYPLLGKLLFSDVSVDATTGSVTLRALFPNPDRTLLP
                     GMYVRARLEQAVDEQAIVIPQQAVMRDQNGASVFVVGADDKVVARPVKTGAAQGNDWV
                     ITDGLKSGDRVIVEGLQKTRPGAPVKPVPWQGPVGEAATQAQSPSGQGAATKVN"
     gene            2018..2650
                     /locus_tag="VFA14_03625"
     CDS             2018..2650
                     /locus_tag="VFA14_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009239358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HZS80505.1"
                     /translation="MARSTKEEALETRARILDAAEQVFYEHGVSHTSLADVAKAADVT
                     RGAIYWHFRNKSDLFDAMCERVRLPMETMMEDNAVERVADPLGQFTKVGVYVLKQAVN
                     DPRCRKVFDIIFNKCEFVDNNDPILMRQRECRMQGMLRMEQTLKNAIARGQLPAGINI
                     HLACLMVHAMVSGLLTDWFFAQDSFDLEQEAEKLFAACIHALKTAPTLLI"
     gene            complement(2642..>3005)
                     /locus_tag="VFA14_03630"
     CDS             complement(2642..>3005)
                     /locus_tag="VFA14_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HZS80506.1"
                     /translation="IALADKARADLENARRTAALDARQAYLSVSNGLSQVKAYEAAEI
                     SSLSALESNRLGYQVGVRINIDVLDAQRQLFATRRDLARARYDTLMNGLKLKAAAGNL
                     GEDDLVNINRLLEAPASD"
BASE COUNT          608 a          864 c          882 g          651 t
ORIGIN      
        1 cgttcagcac gttacgcgac agatagtcgc ccagcgccat cggatcgagt ttgccgtcgg
       61 tcgaagacaa gctgatgaac agcaggaagt tgctgcgcga cttggtgacc tgcacgcctt
      121 gttgcgttac cgctgccggc agacgcgctt ccacccgctt caaacggttt tgcacgtcga
      181 ctgccgccag atcgggattg gtttcgggcg agaacgtcac cgtgatctgc acctggccat
      241 tggcctgact ttgcgactcg atatattgca ggccgtcggc gccgttcatt tcctgctcaa
      301 tcaggctggt taccgcatcg tccagaacct gcgcggtcga gccgggatag gccgccgaaa
      361 tcacgatggt cggcggcgcg atgctcggat actgggcgac aggcaggctg gtgatggcca
      421 gcgcgcccgc cagcatgatg aagattgcca acacccacgc aaaaacaggg cgatcgataa
      481 agaactttcc catctgacag cctcttagtt aactttggtt gcggcgccct gcccggatgg
      541 cgactgtgct tgggtggcag cctcaccgac cggaccctgc catggaaccg gcttcaccgg
      601 cgcgcctggt ctggtttttt gcaaaccctc gacgatcacg cggtcaccgc tcttcaggcc
      661 gtcggtaatg acccaatcgt taccttgggc ggcaccggtc ttgaccggac gcgcaacgac
      721 cttgtcatct gcaccaacca caaacaccga cgcgccgttc tggtcgcgca tgaccgcttg
      781 ttgcggtatg acgatggcct gttcgtcgac agcctgttcc agccgggcgc gtacatacat
      841 gcctggcagc agtgtgcggt ccggattagg gaatagcgcg cgcagggtca ctgagcctgt
      901 cgtcgcatcg acgctcacgt cggagaacag caacttgccc agcaaaggat aaggacggcc
      961 gtcctcggtg accagcgaga ccctggcttt gtccttgccg accgttttca attgtccgct
     1021 ggccatggct tgttgcagtt tcagcaggtc agtgctcgat tgcgtcaggt tgacgtagat
     1081 cgggtcaagc tgctgcacga cggccagctg ggtcgcctca ccctgaccga ccagcgcgcc
     1141 ttcggtcacc agcgcgcgtc cgatgcggcc ggagatcggc gaggtgacag tggtgtagcc
     1201 caggttcagg ccggcgatct gcatggctgc ttcggcttgc ttctgcgcgg caacggcatc
     1261 gtcatattct tgcttgctga cggcgttgac ttcggccagc ggcttgtagc gctggacctt
     1321 gagcgtggct tgcgtcagcg tggcttgcgc gctgtcgtaa gaggccttga acgtagccgg
     1381 gtcgatgtgg tacagcacat cgccggcctt gacctcgctg ccttcgcgaa agctgcgttt
     1441 gagtatgata ccggcgacac gggcgcgcac ttcggcgatg cgggtcgctt ctaggcggcc
     1501 gggcagctca ttggtgaccg tgatgcgctc aggcgcgatc gtcacgacgg acgcttcgcc
     1561 gggaggcatg tttgcgcccg gttgggtact ctgtttttga ccgcagcctg caaaagcggc
     1621 cagtacagca acaatcaggg tgagatgtgt gaaacgacta actgagctca taaatatctc
     1681 gcacggtaag taaagttgac gctgcagcgg taattcgaat acggaatacg ataaaaatac
     1741 tgagacattg aagaatgata ctgcggcatg gagaagacag cggacagagt aaaacgacga
     1801 agcggtgatt aaaatctggt gctattatac atacattcct gaatgtatat atctcgcata
     1861 tttatcgcaa tatttctaaa tataagtatg catagacgca cggatttttg ccagaaatct
     1921 cctcttgtca ggggagcctg tgatgatctg tcttcattgc gcaacgatgg tgtagggaga
     1981 tagcaatgac attaacgatg cggacggtgt cctgaccatg gcgcgctcaa ccaaggaaga
     2041 agcgctcgaa acccgcgcgc gcattctcga tgccgccgag caagtatttt atgaacatgg
     2101 cgtttcgcat acctcgttgg ccgatgtcgc taaggcggcg gatgtcacgc gcggcgccat
     2161 ctattggcat ttcaggaaca agagcgacct gttcgacgcc atgtgcgagc gtgtacgcct
     2221 gccgatggag acgatgatgg aagataacgc cgtcgagcgc gtcgccgatc cgctcggtca
     2281 gttcaccaag gttggcgttt atgtcttgaa gcaggcggtg aacgacccgc gttgccgcaa
     2341 agtgttcgac atcatcttca acaagtgtga attcgtcgac aataatgatc ccatcctgat
     2401 gcgccaacgt gaatgccgca tgcagggcat gctgcgcatg gagcagactc tcaagaatgc
     2461 catcgcgcgc ggccagttgc cggctggcat caacattcat ctggcctgtc tgatggtgca
     2521 tgccatggtc agcggcttgc tgaccgactg gttctttgca caggatagct tcgacctcga
     2581 gcaggaggcg gaaaaactgt ttgccgcctg cattcatgca ctcaagacgg ccccgaccct
     2641 gctaatctga cgccggcgct tccagcagcc gattgatgtt caccagatca tcttccccca
     2701 gattgccggc cgccgctttc agtttcagac cgttcatcaa cgtgtcgtag cgcgccctgg
     2761 ccagatcgcg tcgcgtcgcg aacaattgtc gttgcgcatc gagcacatcg atattgatgc
     2821 gcacaccgac ctgatagccg aggcgattcg attccagcgc cgacagactg gaaatttccg
     2881 ccgcctcata agcctttacc tgcgacagac cattgctgac gctcaggtaa gcctggcgcg
     2941 catcgagcgc ggctgtgcgg cgcgcatttt ccagatcggc acgggctttg tcggccagcg
     3001 cgatc
//
LOCUS       DASRDH010000159         2299 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1391967,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000159 DASRDH010000000
VERSION     DASRDH010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1391967"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="VFA14_03635"
     CDS             complement(<1..237)
                     /locus_tag="VFA14_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011809012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR-associated endonuclease Cas1"
                     /protein_id="HZS80507.1"
                     /translation="MVEIADDRRHLFLSRGFLVVQDTEDERKELGQIPLDDIAAVIAN
                     AHGLSYTNNLLVSLAERCAPFVLCAANHNAVGMLL"
     gene            complement(382..1980)
                     /locus_tag="VFA14_03640"
     CDS             complement(382..1980)
                     /locus_tag="VFA14_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS80508.1"
                     /translation="MLRTMRYRLALDLGSTSLGWALIRLKDSKPCAVIKAGVRVFPDG
                     RNPKDGSSLAVTRREARAMRRRRDRLLKRKNRLIEALTDLGFFPASAPERAALVTLDP
                     YALRARGLDEALSAAEFARALFHLNQRRGFQSNRKTDKKDNDSGALKRAIKESREKLA
                     QENCRTLGEWLAQRHAAGESVRARLRGKTQKDKAYDYYADRAMVAHEFDTLWAAQAAR
                     NPLLFSEAARLRLRDVLLHQRPVKPVKPGRCTLLPEELRAPLALPSTQRFRIYQELNH
                     LRILMPDLQEQALTLAGLLESKSELSFSAMTKVLKLSGTTSFNLADIKRDRLNGNKTS
                     AILSKPEHFGKAWSGFDLARQDEIVTRLLDEASESALIDWLQTHTGVDEAQAEKIANT
                     RLPEGYGRLGRTALGRILPVLMADVVTYDKAVTRAGFDSHSALSHALQTGEIMESLPY
                     YGDPLRRHVAFEKEKPRNDEEGRRLEEITPSAMDAIRQTAGDAPDEEGLSASGDRWRG
                     ANDGDRQDQRKRSDRYGRASRGER"
BASE COUNT          449 a          718 c          631 g          501 t
ORIGIN      
        1 cagcagcatg ccgacggcat tgtgattggc ggcgcacagc acgaacggcg cgcagcgctc
       61 agccagggag accagcaggt tgttggtata gctcaaacca tgcgcattgg cgatcaccgc
      121 cgcgatatcg tccaggggga tctgtcccag ttccttgcgt tcgtcctccg tatcctgcac
      181 caccaggaag ccacgcgaaa gaaagagatg ccggcgatcg tctgctattt ccaccatgcg
      241 gcctatcatg acggtcagtc tttgaatccg ggatcgcgca gcttgccggt tgcagagacc
      301 gtgcagcgct ggcctttggc tttttgcagg gagccggctg ttttgtatgt ataagcgaag
      361 gtcttgtccc tatttctgga gtcaacgttc gcctcgtgat gctctgccat aacgatctga
      421 ccgtttgcgc tgatcttgac gatcaccatc gttcgcaccg cgccatcgat ctccagacgc
      481 agataatcct tcttcatcag gcgcatcacc agcggtttgc cggattgcgt ccattgcgga
      541 tggcgtaatt tcttccaacc ttcttccttc ttcatcgtta cgcggctttt ccttctcgaa
      601 agccacatgg cggcgcaagg ggtcgccgta atacggcagc gactccatga tttccccggt
      661 ctgcaaggca tgcgacaagg cgctgtggct gtcgaatccg gcgcgggtca ccgccttgtc
      721 gtaggtcacg acatccgcca tcagcaccgg cagaatgcga cccaatgcag tacgccccaa
      781 tctgccgtat ccttctggca aacgggtatt ggcgattttt tccgcctgcg cttcatcgac
      841 gccagtatgc gtttgcagcc agtcgatcaa tgcgctttcg ctggcctcat ccaatagcct
      901 ggtgacaatc tcatcctgcc gcgcaaggtc gaaaccggac catgccttgc cgaaatgttc
      961 tggcttcgac aaaatggcgc tggtcttgtt gccgttcaag cgatcgcgct tgatgtctgc
     1021 cagattgaag ctggtcgtac cagataattt caataccttg gtcattgcgc tgaagctgag
     1081 ttcgctcttg ctctccagca gcccggccag cgtcaaggcc tgctcctgca aatcaggcat
     1141 caggatgcgc agatgattca actcctgata aatgcggaaa cgctgcgtac tcggcaaagc
     1201 cagcggcgcg cgcagctcct ccggcagcaa ggtgcagcgg cccggcttca ccggcttcac
     1261 cgggcgctga tgcagcagca catcgcgcaa ccggagacgc gcggcttccg aaaacaggag
     1321 cggattgcgc gccgcctgcg ccgcccacaa cgtatcaaac tcatgcgcga ccatggcgcg
     1381 gtcagcgtaa taatcgtaag ccttgtcctt ttgcgtcttg ccacgcaaac gcgcgcggac
     1441 actctcgccc gccgcatggc gttgcgccag ccattcaccg agcgtgcggc agttttcctg
     1501 tgccagcttc tcgcgggatt ccttgatggc gcgtttcaat gcgccgctgt cattgtcttt
     1561 tttgtcggtt ttgcgattgc tctgaaagcc gcgacgctgg ttcagatgaa acagggcgcg
     1621 cgcgaactcg gccgcgctca aggcttcgtc cagccccctg gcgcgcaagg cataaggatc
     1681 gagcgtcacc agggcggcgc gctccggtgc cgacgccgga aaaaaaccaa gatcggtcaa
     1741 ggcctcgatc aagcggttct tgcgtttgag caggcgatca cggcgacggc gcatggcgcg
     1801 cgcctcacgc cgcgtgacag cgagggaaga accatctttg ggattgcgcc catccggaaa
     1861 aacacgcacg ccagctttga tgacggcgca aggcttgcta tcctttaaac gaatcaaggc
     1921 ccagccaagc gaagtagacc ccaggtcgag cgccagacga tagcgcatgg tgcgtaacat
     1981 attcctcctc cgatgaggat gatgcgctga ccaggaatga tggcctgcaa ccggaagctg
     2041 cttatgctat ataatgcatt ccacgcggcg catgctccag tcaccaagtc cgctatcctt
     2101 gtttcaaccc catttgcctg cgactggcgc cccttgcggc gacaggccga tattttagaa
     2161 ttccaccatg tcattctcct ctctcggtct ttccggggaa atcgttcgcg ccgttactga
     2221 acacggttat accactccta caccgataca gctgcaagcc atccccgcgg tgctcgccgg
     2281 cggcgatctg ctggccggc
//
LOCUS       DASRDH010000160         2734 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_882503,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000160 DASRDH010000000
VERSION     DASRDH010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2734
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_882503"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1779)
                     /gene="bamA"
                     /locus_tag="VFA14_03645"
     CDS             complement(<1..1779)
                     /gene="bamA"
                     /locus_tag="VFA14_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HZS80509.1"
                     /translation="MNLHSAHFFVTTLARSLIAAVLVFCSHHAFAVAPFVVKDIRVEG
                     IQRTEAGTVFSYLPVRVGDTFDDEKGTAAIKALYATGFFKDVRIETEGNVLVVQVEER
                     PAIASVDFTGTREFDKNQLIKALKEIGVAESRIFDRSQVDRAEQELKRQYLSRGLYGA
                     TVATTVTPVERNRVAVSFAVNEGEIARIKQINIIGNKAFLDSTLRSQLKLTAPGWFTW
                     YTKADQYSKQKLAGDIESLRSYYLNRGYLEMQVESTQVSITPDKQDIYITININEGEK
                     YTVSDIRLDGEMFGMQDDLAALVQLKKGDVYSGAKLTDSARKIGERLGNFGYAFANVN
                     ANPDIDREKKEVAFTIMIDPGKRVYVRRINIAGNTKTRDEVIRREFRQFENSWYDGEK
                     IRLSRDRVDRLGYFKEVSIETPEVPDSTDQVDVNMRIEEKPTGNVMLGAGFSQTDKLS
                     LVGSISQENAFGSGNTIGIDVNTSSRYRTVAVSQTNPYFTDDGVSRTYEMFLRTMRPS
                     AYTRGDYRVKTLGGSIRFGVPFSELDRVFFGVGVENTGVDTDGSSPSLYRQYVRDFGS
                     GEVAGCDVLSSSSCGIGTARTTSFPFT"
     gene            complement(1863..>2734)
                     /gene="rseP"
                     /locus_tag="VFA14_03650"
     CDS             complement(1863..>2734)
                     /gene="rseP"
                     /locus_tag="VFA14_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HZS80510.1"
                     /translation="SWSEVRWELMQHVLEKTPARLDVERHYPGGRLSDTVNLPLDSVS
                     DEDLEGDFLSRLGLSLARPPAILGRIMDNGPAMQAGLQMGDLILRVDDTPVRDGLALV
                     ELVRASPGKAVQLHGLRGQRELHVRVTPEMFDDNGQRVGRIGVEVLLAPEVAVISDTP
                     WTALVKGVRKTWDTGVMTLKMIGRMIVGQVSWKNITGPITIADYAGQTAKVGLVSYLN
                     FIAFISISLGVMNLLPIPVLDGGHLLYYSLEILTGRPVSERFGEIAQRAGLGILMMLM
                     LVAVFNDIVRLMF"
BASE COUNT          605 a          822 c          713 g          594 t
ORIGIN      
        1 cgtaaacggg aaactggtcg tccttgcggt gccgatgccg caggaactgc tggaaagcac
       61 atcacagcct gccacctcac cgctgccgaa atcgcgcaca tactgacggt acaggcttgg
      121 gctgctgcca tctgtgtcaa ccccggtgtt ttccacaccg acgccaaaga acacgcgatc
      181 caactcggag aatggcacgc cgaacctgat gctaccgccc agggttttga cgcggtagtc
      241 gcccctggtg taggccgatg gacgcatcgt gcgcaaaaac atttcatacg tgcggctgac
      301 gccgtcatcg gtgaaatagg gattggtctg cgacaccgca accgtgcgat aacgcgaact
      361 ggtattgacg tcgatcccga tggtgttacc gctgccaaag gcattttcct gcgaaatcga
      421 accgaccaga ctcagcttgt cggtttgtga gaagccggcg ccgagcatga cgttgccggt
      481 cggcttttct tcgatcctca tgttgacgtc gacttgatcg gtgctgtccg gcacttccgg
      541 cgtctcgatg ctcacttcct tgaaataacc cagacgatcg acgcggtcac gcgacagcct
      601 gatcttctcg ccgtcgtacc aggagttctc gaactgacgg aattcgcgac ggatgacttc
      661 atcgcgcgtc ttggtattgc cggctatgtt gatgcggcgc acatacacac gcttgcccgg
      721 gtcgatcatg atggtgaagg caacctcctt cttctcgcga tcgatatccg gattggcatt
      781 cacattggcg aaggcgtaac cgaaattacc cagacgttcg ccgatcttcc tggcgctgtc
      841 cgtcagcttg gcgccggaat acacatcgcc ttttttcaat tgcaccaatg ctgccagatc
      901 atcctgcata ccgaacattt cgccgtccag cctgatatcg gaaaccgtat atttttcgcc
      961 ttcattgata ttgatggtga tataaatatc ctgcttgtcc ggcgtgatcg acacctgggt
     1021 cgattcgacc tgcatttcca gataaccacg attgagataa tacgagcgca gcgactcgat
     1081 gtcgcctgcc agcttctgct tggaatactg atcagccttg gtgtaccagg tgaaccagcc
     1141 cggcgcggtc agtttgagct gactgcgcag cgtgctatcc aggaatgcct tgttgccaat
     1201 aatgttgatc tgcttgatgc gggcaatctc accctcgttt acggcaaacg ataccgcaac
     1261 gcgattgcgc tcgaccggcg tcacggtggt ggcgaccgtg gcgccgtaca gaccgcgcga
     1321 cagatactga cgtttcagct cctgttcggc gcgatcgacc tgcgacctgt cgaaaatacg
     1381 cgactcagcg acgccgattt ccttgagcgc cttgatcaac tgattcttgt cgaactccct
     1441 ggtgccagtg aagtcgacgc tggcgatcgc cggacgctct tccacctgaa ccaccagcac
     1501 attgccttcg gtttcgatgc gcacatcttt gaaaaagccg gtcgcataaa gcgccttgat
     1561 cgccgccgtg cctttttcat cgtcgaaagt atcgcccacg cgcaccggca aatagctgaa
     1621 aaccgttccg gcttcagtac gctgtatgcc ttcgacacgg atatccttga ccacgaacgg
     1681 tgcaaccgca aacgcatggt gtgaacagaa taccagtacg gcggcgatca agctgcgggc
     1741 aagggtagtg acaaaaaaat gcgcggaatg taagttcatc gcttatcgct tatcgtttat
     1801 gtcgaacatg ctgcaaccat gttctcgatc acaagccctc cagggcgacc ggtgacgcat
     1861 tttcaaaaca tcagacggac aatgtcgttg aacacggcaa cgagcatcag catcatcaaa
     1921 ataccgagac cggcgcgttg cgcaatctct ccaaaacgtt cggaaaccgg acgccctgtc
     1981 aaaatctcca gcgaataata cagcaaatga ccgccatcta aaaccggaat aggcagtaga
     2041 ttcatgacgc cgagactgat actgatgaag gcgatgaagt tcagatagct gaccagaccg
     2101 actttggcgg tctgccctgc gtaatcggcg atggtaatcg ggccggtaat atttttccat
     2161 gacacctggc cgacgatcat cctacctatc atcttcagtg tcataacgcc ggtatcccag
     2221 gtctttcgca cgcccttgac aagcgctgtc cacggcgtat cgctgatgac ggccacctcc
     2281 ggcgccagca gcacttcgac cccgatgcgg ccgaccctct ggccgttgtc atcgaacatc
     2341 tccggcgtga cccggacatg cagttcacgc tgaccgcgca gaccatgcaa ctgtaccgct
     2401 ttgccgggag aagcgcgcac cagctcgacc agggccaggc catccctgac tggcgtatcg
     2461 tccacgcgca aaatcaggtc gcccatttgc aaacccgcct gcatggcggg gccgttatcc
     2521 atgatcctgc ccaggatggc gggcggacgg gccagagaca aaccgagtct ggatagaaaa
     2581 tcgccttcga gatcttcgtc cgatacgcta tcgagcggca ggttcacggt gtcggacagt
     2641 ctgccgccgg gataatggcg ctcgacatcc agacgcgccg gcgtcttttc gagcacatgc
     2701 tgcatcaatt cccagcgcac ttccgaccag gact
//
LOCUS       DASRDH010000161         5784 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1496936,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000161 DASRDH010000000
VERSION     DASRDH010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 5784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5784
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1496936"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..804
                     /locus_tag="VFA14_03655"
     CDS             <1..804
                     /locus_tag="VFA14_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008327831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DALR domain-containing protein"
                     /protein_id="HZS80511.1"
                     /translation="WDSPWGKGRPGWHIECSAMSGDLLGNHFDIHGGGHDLQFPHHEN
                     EIAQSEGAHRHGFVNYWMHNGFVRIDNEKMSKSLGNFFTIRDVLKKYDPEVVRFFILR
                     AHYRSPLNYADAHLDDARHALTRLYTALKEIPVEDSAPVDWSEVHAQRFANAMNDDFN
                     TPVVIAVLFDLAAEVNRSGSQALARQLRALAGIIGLLQRPAEDYLKVGASSGIEGLAA
                     VEIEARVAARIAARKAGNFTAADAIRAELAAAGVVLEDKPGGLTEWRRC"
     gene            810..1454
                     /locus_tag="VFA14_03660"
     CDS             810..1454
                     /locus_tag="VFA14_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007877336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HZS80512.1"
                     /translation="MVKAHQTDPSISVPPYWEEAKAELMKRDRIMRKLIPQFGDLQLT
                     ARGDAFTTLARSIIGQQISTRAAQTVWQRFLETCTRCTPIQVIKAGNEKLLACGLSRR
                     KAEYILDLANHFKARTVQPCKWVEMADEEVIADMLRIRGIGRWTAEMFLIFNLLRPNV
                     LPLDDLGLLKGISSNYFSGEPVSRSDAREVAVNWEPWRTVATWYLWRSLDSVAS"
     gene            1658..1825
                     /locus_tag="VFA14_03665"
     CDS             1658..1825
                     /locus_tag="VFA14_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS80513.1"
                     /translation="MTVGIAVRIAARKAGNFTAADAIRVELAAAGVMLEDKPHGLLYC
                     RLFMKVRKGIR"
     gene            1822..2802
                     /locus_tag="VFA14_03670"
     CDS             1822..2802
                     /locus_tag="VFA14_03670"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HZS80514.1"
                     /translation="MSKSTTTFLGFEQAIAELDAKIEELRFVQDDSAVDISEEIERLV
                     KKSQQLTKDIYAKLTPWQVSQIARHPQRPYTMDYVNEIFTDFHELHGDRTYADDLSIV
                     GGLARFKGQACMVIGHQKGRDTKERAMRNFGMPKPEGYRKAMRLMKLAEKFDLPIFTF
                     VDTPGAFPGIDAEERGQSEAIGHNLYVMAELKVPLIATIIGEGGSGGALAIAVGDSVL
                     MLQYATYSVISPEGCASILWKTADRAPDAAEALGLTAHRLKAMGLIDKIVNEPLGGAH
                     RDPKQMAFFLKRALADTLRQFQGAKTRELLAARHEKLMSYGKFKELDTKE"
     gene            2903..4354
                     /gene="tilS"
                     /locus_tag="VFA14_03675"
     CDS             2903..4354
                     /gene="tilS"
                     /locus_tag="VFA14_03675"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007877340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HZS80515.1"
                     /translation="MPASPSSATALSLSQELVRVLQALAASYMHDSRFAEVFPTMAIA
                     CSGGLDSSVLLDLAATFAREQGVRLFVFHIHHGLSANADDWQEHCRRAGERVSAVFDT
                     RRITLEDASKSGIEEAARIGRYHALGDLCRVHGVTLLLTAHHQDDQAETILLQLLRGC
                     GVAGMSGMDQVNTAADLLDNRELLLARPLLHASRAALEHYAKVHSLAFVEDESNRDTR
                     YARNALRHRIMPALTEFFPGFQERFARAASHARSAQALLATLARQDLAACLEGEYLSV
                     PLLRQLDVDRVDNLLRHWLAWRGLRMPSAAWLLELRTQLLEAKADAQLCVTHPDCHIR
                     RYRERVFLTPRRPALDEDTQPQAFVWRGEAQRHFPRFAGSLFFDVSDQGFDAAWLREQ
                     HLSLRLRRGGERVKLAWNRPGRSVKHHYQALDIPAWKRPYLPMVFAGEQILYAAGIGM
                     DCHAQEETGDSDSAGDIDGNGRCISLRWQSDLR"
     gene            complement(4510..4773)
                     /locus_tag="VFA14_03680"
     CDS             complement(4510..4773)
                     /locus_tag="VFA14_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007877341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS80516.1"
                     /translation="MKSKSIIVAALFSALTLPAFAEDAAHAATTPAPKTGHAAGEHKK
                     EKHASKTGHAAEHKKNKHTSKTEHAAEAPAEKPATDAMPAKAH"
     gene            complement(4958..>5784)
                     /locus_tag="VFA14_03685"
     CDS             complement(4958..>5784)
                     /locus_tag="VFA14_03685"
                     /inference="COORDINATES: protein motif:HMM:NF014335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HZS80517.1"
                     /translation="VDIHTIDYSYWDPAGLGAGIENLQIGQNLKLSYALFIKDSIAQS
                     HYAPRHDLQLSGMYTNPRGELSLGLSFIPQRGNIQMPDGAYQPTHAGWTVTMQHIQTE
                     LLGGKHKFALQFGKGPGSSLIGTGALTQSADFKAWRVINALDWQLTRDFSGMVDAVCQ
                     RNIAPGASQTWVSFGARPVYALTRHLKAVLDFGHDRVKPDNGATRTLNKVTTAIVVGK
                     DRGFWSRPELRFFYTHAHWNKATQDAAALGDALSDTGVFGAARTGSTAGVQFETWF"
BASE COUNT         1136 a         1602 c         1783 g         1263 t
ORIGIN      
        1 tgggattcac cgtggggtaa ggggcgcccg ggctggcata tcgaatgctc ggccatgtcg
       61 ggcgatctgc tgggcaatca tttcgacatt catggcggtg gccacgatct gcaattcccg
      121 caccatgaga atgaaattgc ccagtcggag ggcgcgcatc ggcatggctt cgtcaattac
      181 tggatgcaca acggctttgt gcgtatcgac aatgaaaaaa tgtcgaaatc gctgggaaat
      241 ttctttacca ttcgagatgt tctgaagaaa tacgatcccg aggtagtgcg cttttttatc
      301 ctgcgcgccc actatcgcag tccgttgaat tatgcagacg cacatctcga cgatgcacgc
      361 catgccttga cccgtcttta caccgcattg aaagaaatac cggtggaaga cagcgcgccg
      421 gtcgattgga gtgaagtgca tgcgcagcgt tttgccaacg cgatgaacga cgatttcaac
      481 acgccggtcg tgattgcggt attgttcgat ctggccgctg aagtcaacag gagtgggtcg
      541 caggctctgg caagacaatt gagagcgctg gcgggtatca ttggactgtt gcagcgtccg
      601 gccgaagatt acctgaaggt cggggccagc agcggtatcg aaggacttgc ggcagtagag
      661 atcgaggcaa gggttgcggc gcgcatcgct gccagaaagg ctggaaattt taccgcagcg
      721 gatgccattc gcgccgaact ggccgccgcc ggcgtggtac tggaagacaa gccgggcgga
      781 ctgaccgaat ggagaaggtg ctgagaagaa tggtaaaagc gcatcagact gatccgtcga
      841 tcagtgtccc tccgtattgg gaggaggcca aggctgaatt gatgaagcgc gaccgtatca
      901 tgcgcaagct gatcccgcaa ttcggcgatc tgcaactgac cgcgcgcggc gatgcgttta
      961 ccacgctggc gcgttccatc atcggccagc agatttccac cagagcagcg cagacagtgt
     1021 ggcaacgctt cctggagaca tgcaccagat gcacccccat acaggtgatc aaggccggca
     1081 acgaaaagct gctcgcctgc ggtctgtcca ggcgcaaggc ggaatacatt ctcgacctcg
     1141 ccaatcactt caaggccagg accgtccagc cgtgcaaatg ggtggagatg gcagacgaag
     1201 aggtgattgc cgacatgctc cggatccgtg gcatcggtcg ctggacggcc gaaatgtttc
     1261 tgatattcaa tctgttgcgc ccgaatgtcc tgccactgga cgacctgggc ttgctcaaag
     1321 gcatcagcag caattatttt tccggtgaac cggtctcacg cagcgatgcg cgcgaagttg
     1381 ccgtcaactg ggagccatgg cgtaccgtgg cgacctggta tctgtggcgc agcctggatt
     1441 cggtcgcctc ttgacgaatg aagtgagtgc ggctggcgat gcggcgcgga ccaggcaagg
     1501 agcttacgga tgaagggcat tctctcgcgc catctcccgc cacaatcctg tcccactccc
     1561 gcctggccgc gcgctgcgcc gccgttttgt tctggtagac cttgggcaac tttttgatat
     1621 tgcctagctg gccggccagc tctgtgtcgc aggacttttg acagtaggga tcgcggtgcg
     1681 catcgctgcc agaaaggctg gaaattttac cgcagcggat gccatccgtg tcgaactggc
     1741 tgcggctggc gtgatgctgg aagacaagcc gcacgggctg ttatactgcc gcctgttcat
     1801 gaaagtgcgt aaaggaatac gatgagcaag tcgacgacga catttctggg gttcgagcag
     1861 gcgatcgccg agctggacgc taaaatcgag gagttgcgtt ttgtacagga cgattcggcg
     1921 gtcgatatct cggaagagat agaacgcttg gtcaagaaga gccagcagct gaccaaagat
     1981 atctatgcca agctgacccc atggcaggta tcgcagatcg cgcgccatcc gcagcgtcct
     2041 tacaccatgg actatgtgaa tgaaatcttc accgactttc atgaattgca cggcgatcgt
     2101 acctatgccg atgatttgtc catcgtcggc ggtctggcgc gtttcaaggg tcaggcctgt
     2161 atggtgatcg gccaccagaa gggacgcgac accaaagagc gcgccatgcg caacttcggc
     2221 atgccaaaac cggaaggcta ccgcaaggcc atgcgcttga tgaagctggc cgaaaaattc
     2281 gatttgccca tttttacctt cgtcgatacg ccgggcgcat tccccggcat cgatgccgaa
     2341 gagcgtggcc agtccgaagc gatcggccac aatctgtacg tcatggcgga actgaaagtg
     2401 ccgctgatcg ccaccatcat cggcgaaggc ggctctggtg gcgcgctggc gattgctgtc
     2461 ggcgactccg tgctgatgct gcaatacgcg acttactcgg tgatttcgcc ggaaggctgt
     2521 gcctcgatcc tgtggaagac cgccgaccgc gcgcccgatg ccgccgaagc gctgggtctg
     2581 accgcgcatc gcctgaaagc catgggtctg atcgacaaga tcgtcaatga accgctgggc
     2641 ggtgctcatc gtgaccccaa gcaaatggcg ttttttctca agcgtgcgct ggccgacaca
     2701 ctacgccagt tccagggcgc caagaccagg gagctgctgg cggcgcgtca tgaaaaactg
     2761 atgagttacg gcaagttcaa ggaactcgat acgaaagaat aagcaccgct tcgctgcggc
     2821 aagaacatgc agaaaccggc aggcgcatag gtctgccgtg ccgcccatcg tcgttaccgt
     2881 agtcagcagt ctctgtgtcg ccatgcctgc ttccccctct tctgctactg ctctctccct
     2941 ttcacaggaa ctggtgcgtg tcctgcaggc tttggctgca tcgtatatgc atgacagcag
     3001 gtttgccgaa gtatttccga cgatggccat tgcctgcagc ggcggtctcg attcctccgt
     3061 cctgctggat cttgctgcga cgtttgcgcg cgagcagggc gtgcgcctgt tcgtttttca
     3121 tattcatcat ggtctcagcg ctaatgccga tgactggcag gaacattgtc gccgcgcagg
     3181 cgagcgtgtc agcgccgttt tcgatacgcg caggatcacg ctggaggatg ccagcaaaag
     3241 tggaattgaa gaagcggcgc gcatagggcg gtatcacgcg cttggcgatt tgtgccgcgt
     3301 gcatggcgtc accttgctgt tgaccgcaca tcatcaggat gatcaggccg aaacgatcct
     3361 gttgcagctc ttgcgcggct gcggcgtggc aggcatgtcg ggcatggatc aggtcaacac
     3421 ggcagcggat ctgctcgaca atcgcgagct gctgctggcg cgtccgctgc tgcatgcttc
     3481 aagggcggcg ctggaacact atgccaaagt acactcgctg gcgtttgtcg aagatgagtc
     3541 caaccgtgat acgcgctatg cgcgcaatgc cttgcgccat cggatcatgc cggcgttgac
     3601 ggaatttttt cctggttttc aggaacgttt cgcgcgtgcg gcgagccatg cgcgatcggc
     3661 gcaagctctg ttggccacgc tggcgcgtca ggatctggcc gcctgtctgg agggggaata
     3721 tctgagcgtg ccgctgttac ggcaactgga tgtggacagg gtcgacaatc tcttgcgtca
     3781 ttggttggcc tggcgtggtt tgcgcatgcc atcggcggcc tggttgctgg agctgcgcac
     3841 gcaattgctg gaggcaaaag ccgatgcgca gttatgtgtc acccatccgg attgtcatat
     3901 ccggcgctat cgcgagcgcg tgttcctgac gccgcggcga ccggcgttag atgaagacac
     3961 acagccgcag gcgttcgtct ggcgcggtga agcgcagcgg cacttcccgc ggtttgccgg
     4021 cagcctgttt ttcgatgtgt cggatcaggg attcgatgcc gcctggctgc gcgaacagca
     4081 tctgtcgctc cgtctgcgcc gtggcggcga gcgtgtcaag ctggcgtgga atcgtccggg
     4141 caggagtgtg aagcatcact atcaggcact ggacattccc gcctggaagc ggccatatct
     4201 gccgatggtg tttgccggcg agcagattct ctatgctgcc gggatcggca tggattgcca
     4261 tgcccaggaa gaaaccggcg acagcgatag tgccggcgat atcgatggaa atggtcgctg
     4321 catcagcttg cgatggcagt cggatttgcg ctgatcatgc cgacgataag atcggggtgg
     4381 cgtatgcaat cgtcatccca tgaaaaaacg tcttgccaca tgcatggcaa gacgtttggc
     4441 ttcattccct gagatccgcg ttgcgaccag tctgttctgt tttcggactg gcgcaacgtg
     4501 ccggtctgct taatgggcct tggccggcat agcgtcagtt gccggttttt ctgctggcgc
     4561 ttcggcagcg tgttcagtct tggatgtgtg tttgttcttc ttgtgttcgg cagcgtgtcc
     4621 agtcttggat gcgtgctttt ccttcttgtg ctcgccagca gcgtgtccag ttttgggcgc
     4681 cggcgtcgtt gccgcatgtg cagcgtcttc agcgaacgcc ggcagtgtca gtgcagagaa
     4741 cagggcagca acgatgatgg acttggattt catggtagtt ttccttaaaa aattgattga
     4801 cctcggcgtt tttcgcgcca gaaatgccga aaaactgtca gggcgctggt acgctgctta
     4861 ttataagacc agtctgatta taagaccagt ctgctgcttg caggcaaact tcaggctggt
     4921 gtgtgttttt tctggcaact tcatctgatc ctgctgatca gaaccacgtc tcgaactgta
     4981 cgccggccgt cgaacccgtg cgcgccgcgc cgaacacgcc ggtatcggat agcgcgtcgc
     5041 ccagcgcggc agcatcctgc gtcgccttgt tccagtgcgc atgcgtataa aaaaagcgca
     5101 gctctggacg cgaccagaaa ccgcggtctt taccgaccac gatagccgtg gtgaccttgt
     5161 tcagggtgcg ggtggcgccg ttgtccggct tgacccggtc atgaccgaag tccagcaccg
     5221 ccttcaggtg tcgtgtgagc gcgtagaccg ggcgtgcgcc gaacgacacc cacgtctgtg
     5281 atgctccggg cgcgatgttg cgctgacaga ccgcgtcgac catgccgctg aagtcgcgcg
     5341 tcagttgcca gtcgagcgca ttgatcacgc gccaggcctt gaagtccgcc gactgcgtga
     5401 gtgcgccggt gccgatgagg ctggatcccg gccccttgcc gaactgtagt gcaaacttgt
     5461 gcttgccgcc gagcagttcg gtctggatat gctgcatcgt cacggtccag ccggcatgcg
     5521 tcggctgata cgcgccgtcc ggcatctgta tgttgccgcg ctgcgggatg aatgacagcc
     5581 cgagtgatag ttcaccgcgc ggattggtgt acatgcccga caactggaga tcgtgacgag
     5641 gcgcgtaatg tgactgcgcg atcgaatcct tgatgaagag cgcatagctg agcttcaggt
     5701 tctgaccgat ctgcaggttt tcgatgccgg cgcccaggcc tgccggatcc cagtacgaat
     5761 agtcgatcgt atgaatgtcg acct
//
LOCUS       DASRDH010000162         5867 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1575754,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000162 DASRDH010000000
VERSION     DASRDH010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 5867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5867
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1575754"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="VFA14_03690"
     CDS             complement(<1..439)
                     /locus_tag="VFA14_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HZS80518.1"
                     /translation="MTIVSSDLLTSMNGMSAAASSTSSLKDNSPDAIQNRFLKLLTVQ
                     MKNQNPTNPLDNSQLTVQLAQLSTVSGISQLNTTLASLMSNIQSAQSLQSASMIGRSV
                     LAPGSSVALISDTQTAADGTSTTSQRAVFGVRLEGHADSLKVNI"
     gene            complement(454..858)
                     /gene="flgC"
                     /locus_tag="VFA14_03695"
     CDS             complement(454..858)
                     /gene="flgC"
                     /locus_tag="VFA14_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007882609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HZS80519.1"
                     /translation="MSLFHIFNVAGSAMSAQSQRLNAVASNIANADSATSPNGQPYKA
                     KQVVFKTVPYGNIENSGVKVDRVVEDQVPPKMVYDPKHPMADINGYIAMPNVNPVEEM
                     VNMISASRSYQTNVETMNTAKNMLMKTLTIGS"
     gene            complement(864..1286)
                     /gene="flgB"
                     /locus_tag="VFA14_03700"
     CDS             complement(864..1286)
                     /gene="flgB"
                     /locus_tag="VFA14_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008328136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HZS80520.1"
                     /translation="MVAKLDDYLRFNETALNLRAQRQQLLASNIANADTPNYKARDID
                     FAKTLQTSIEGKSAKLPSVDLVRTSPGHIAGKPINGGGIGGSPLLYRTVAQGSVDGNT
                     VDMDVERNQFTDNAVRYEAGIASVNGQIKMLLAAVQPG"
     gene            1578..2228
                     /gene="flgA"
                     /locus_tag="VFA14_03705"
     CDS             1578..2228
                     /gene="flgA"
                     /locus_tag="VFA14_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HZS80521.1"
                     /translation="MPGVPTASSRQDLGALQKIAEQFIKIQTTGMSGTVSAAVDPVDN
                     RLSLAACTAPQAFLPNGSRLWGRTSIGIRCTAPSPWTVYLRATVQVIAEYIVTTAPLA
                     QGQTIGPDNISKVKGDLSNLPNGVITDASQAIGRIANTSLAAGAPLRLDALRGNRVVQ
                     QGQVVRVVSTGPGFQITTEGRALTNASEGQIAQAKTPAGQVISGIAKTGGILEINY"
     gene            2361..2678
                     /gene="flgM"
                     /locus_tag="VFA14_03710"
     CDS             2361..2678
                     /gene="flgM"
                     /locus_tag="VFA14_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007882621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HZS80522.1"
                     /translation="MNVNDALKIKNGTNLPKITRLQARTSTANSARTDNTNINSANVQ
                     LSVQCQSLGANVLHTDASFDAKKIAEIKAAIANGTFQIDPEKIASGLLATVKNLINTR
                     RKT"
     gene            2683..3165
                     /locus_tag="VFA14_03715"
     CDS             2683..3165
                     /locus_tag="VFA14_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007882622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HZS80523.1"
                     /translation="MTNTSPLLPTRDLAEEKQVVARLVTTLQQEQTLLTGNGDADDIP
                     DVIGEKAKIIAEMARLADQRHKLLAAAGFMASENGMQAWLLQHGTTADKTLWDDLFAL
                     AQTARELNRLNGMLVGKQMAINQSALKILQGHTGGAGAFYGPDGSSSMHNTGRPWGIG
                     "
     gene            complement(3166..3948)
                     /locus_tag="VFA14_03720"
     CDS             complement(3166..3948)
                     /locus_tag="VFA14_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008328132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HZS80524.1"
                     /translation="MYNFNGKADKNDLLQKHASLVRKLAYQMKAKLPPSVEVDDLIQA
                     GMMGLLDVINRYEETHGAQFETYAIQRIRGAMLDELRNSDWLPRSIRQNMRKIENAIH
                     VLMQQLGHPPKEVDVARQLNMSLTEYQDMLAECGGHQLIYYEDFRDSEDGHEHFLDRY
                     QTSQNDDPLQGLIDGDFREAVINAIRVLPEREKILMGLYYEQEMNLKEIGAVMGVSES
                     RVCQLHSQAIARIRSTLREQAWTGLWYLPEKKTAIEDLRSKA"
     gene            complement(3956..4756)
                     /locus_tag="VFA14_03725"
     CDS             complement(3956..4756)
                     /locus_tag="VFA14_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiactivator of flagellar biosynthesis FleN
                     protein"
                     /protein_id="HZS80525.1"
                     /translation="MLERPTPSVFSFFSVLPDAEKDAMLINLAASLAAHDCSVLLVDA
                     CMTPSSVLSRFGGRAVASLLDVACQERVLDEALRRVPEGFVLARLSHKSVSVATHLQA
                     SRLAEIFDVLTDESDVTLVNGELNEDKSLPVPGMEMGGEIVVHMSTSLGSIKAAYALI
                     KSLNDRLGRRPFSLLVTGGSDAEAKMVFANMAQVANRYLAVQLNFLGAIPADEHIRRA
                     SGQGRTVLEAFPLTGAAIAFKRLARQFSLLHLTKGTYGMATDSVGLGA"
     gene            complement(4791..5867)
                     /gene="flhF"
                     /locus_tag="VFA14_03730"
                     /pseudo
     CDS             complement(4791..5867)
                     /gene="flhF"
                     /locus_tag="VFA14_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007882631.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
BASE COUNT         1397 a         1706 c         1469 g         1295 t
ORIGIN      
        1 gaatattgac cttgagacta tcggcatgcc cctccaagcg cacgccgaag acagccctct
       61 gcgaagtcgt cgatgtaccg tctgcggcag tctgggtgtc tgaaatcagg gcgacgctgc
      121 tgcccggcgc caatacgcta cgaccgatca tgctggccga ttgcagagat tgcgccgatt
      181 gtatattcga catcagcgat gccagcgtcg tattgagctg gctgatgccg gacacggtcg
      241 acagttgcgc cagttgtacg gtcagttgcg aattgtccag cgggttggtc ggattctggt
      301 ttttcatctg cactgtcagc aacttcagaa aacggttctg aattgcatca gggctgttgt
      361 ctttcagact cgatgtactg gaagcagcgg ccgacatgcc attcatggac gtcagcagat
      421 cagaagaaac aatggtcatg attaaagcct tccttaagaa ccgatagtca gcgttttcat
      481 caacatattc ttggcggtgt tcatcgtctc gacattggtc tgatacgatc gtgaagcgga
      541 aatcatgttg accatctctt ccaccggatt aacgttcggc atggcgatat agccattgat
      601 gtccgccatg ggatgcttgg ggtcgtacac catcttaggc ggcacctgat cttctactac
      661 ccgatcgacc ttcacaccgc tgttttcgat attgccgtat ggaactgtct tgaagacgac
      721 ctgcttggct ttgtaaggct gtccgttcgg actagtcgcg ctgtcggcgt tggcgatatt
      781 gctggcgact gcattgagac gctgcgactg cgcactcatg gccgaaccgg cgacattgaa
      841 aatatggaaa agagacatga tgatcaacct ggttggacgg cagcaagcag catcttgatc
      901 tgcccattaa cggatgcaat accagcttca taacgcacgg cattgtcggt aaactgattg
      961 cgctcgacat ccatatcgac cgtattacca tccacgctgc cttgggctac ggtacgatac
     1021 agcagcggcg aaccgccgat gccgccgcca ttgatcggct tgccggcaat gtgcccaggc
     1081 gaggtgcgta ccagatcgac cgatggcaat ttcgctgact tgccctctat cgacgtctgc
     1141 agggttttgg cgaagtcgat atcgcgcgcc ttgtagttgg gcgtatcggc attggcgata
     1201 ttagacgcca acaactgttg ccgctgggcg cgcaaattta gcgccgtctc gttaaaccgc
     1261 agataatcat ccagttttgc gaccatcgaa ttctcctgat acattctcca aaagcacgca
     1321 ttacgccgac aatgagattg atataggatg ggatcatacg actccaccca gaactcgata
     1381 gcttgataaa gagataaaac tatgccttgt tcagcctttt gatacaggca gtcaccgata
     1441 ccattggtta tcaagataat tacccttgcg accacgacag tttagaattt ccggtcatga
     1501 aatcatttct caaaacgctg tcattacttc tactgcttca cactttctcc gggttctccg
     1561 ctctggcaca aacaaccatg ccgggtgtcc ccactgcgtc gtcgcgtcag gatctgggtg
     1621 cgctgcaaaa aatcgctgaa cagttcatca aaatccagac caccggcatg tccggcacgg
     1681 tcagcgccgc cgttgaccct gtcgataacc gcttgagcct ggcagcctgc accgcgcccc
     1741 aagcctttct gcccaatggt agtcgcctgt ggggccgaac cagcataggc atccgctgca
     1801 ccgccccttc gccatggacg gtttacctac gcgccacggt gcaagtcatt gccgaataca
     1861 ttgtcaccac agccccgctg gcgcaaggac aaaccatcgg cccggataac atcagcaagg
     1921 tgaagggcga cctgtcgaac ttgccgaatg gcgtcattac cgatgcctcg caagccatcg
     1981 gccgcatcgc caatacttcg ctggccgctg gcgcaccgct acgcctggat gcactgcgcg
     2041 gcaaccgcgt cgtacaacaa ggccaggtag ttcgggtagt atcgaccggc cctggctttc
     2101 agatcacgac cgaaggccgc gcgctcacca acgccagcga aggccagatt gcacaagcca
     2161 aaacacctgc cggacaagtc atcagcggca tcgccaaaac ggggggaatc ctggaaatca
     2221 attactagag cctggtgagc agctcctggc aggaaacagg tttccggtaa gaaatattaa
     2281 agcttgcgaa agaatcgccg ataatccatg caataggtat agatgggact taagcgccct
     2341 aaccattcaa ggatgatgcc gtgaacgtta atgacgcact caaaatcaaa aacggcacga
     2401 atctgccgaa gataacgcgg ttacaggcgc gcacttcgac agcaaacagt gccaggacag
     2461 acaatacaaa tatcaattcc gccaatgtcc agctttccgt ccaatgccag tccctgggcg
     2521 ctaacgtatt gcatacagac gccagtttcg atgcaaagaa aattgctgaa atcaaggcgg
     2581 ccatcgccaa cggcacattt cagatcgatc cggaaaaaat tgccagcggc ctgctcgcta
     2641 ccgtcaaaaa cctgatcaat acccgccgca aaacttaagg taatgacaaa tacatcgcca
     2701 ctactgccca cacgtgatct tgctgaagaa aaacaggtcg ttgcgcgctt ggtcacaaca
     2761 ctacagcaag aacagacttt actcaccggc aatggcgatg ccgatgatat tccggatgtc
     2821 attggcgaaa aagccaagat cattgccgag atggcaagac ttgctgatca acgccataaa
     2881 ctgttagcgg cagccggctt catggccagc gagaacggta tgcaggcctg gctgcttcaa
     2941 cacggcacga ctgccgacaa gactctctgg gatgatctgt tcgcgctggc acaaacggcg
     3001 cgcgaactca atcgcctcaa cggcatgctg gtaggcaagc aaatggcgat caaccagagc
     3061 gcactgaaaa ttctacaagg ccatactgga ggcgccggcg ccttctacgg cccggacggg
     3121 tcgtccagca tgcacaacac cggacgcccc tggggcattg gttgatcaag ccttactccg
     3181 caagtcctcg attgcggtct ttttctcagg taagtaccaa agccccgtcc acgcctgctc
     3241 gcgcagcgtc gagcgtatgc gcgcaatggc ctggctatgc aactggcaga cgcgtgactc
     3301 ggaaacgccc atgactgcac cgatttcctt tagattcatt tcctgttcgt agtacagccc
     3361 catcagaatt ttttcacgct ccggcaacac ccgaatggca ttgatgacgg cttcgcgaaa
     3421 atcaccatcg ataagacctt gcaaagggtc atcattctga ctggtctggt aacggtcgag
     3481 gaaatgctcg tgaccgtctt cactgtcacg gaagtcctcg tagtagatca gctgatggcc
     3541 accgcattcc gccaacatgt cttgatactc ggtcaagctc atattgagct gcctggcgac
     3601 gtccacctcc tttggtggat gccccagttg ctgcatcagg acatggatgg cattttcgat
     3661 cttgcgcatg ttctgacgaa tgctgcgcgg caaccagtca ctattgcgca gttcatccag
     3721 catggccccg cgaatacgct gtatggcata ggtttcaaat tgcgcgccat gcgtttcttc
     3781 ataacgattg atgacatcga gcagccccat catgcctgct tgaatcagat catccacttc
     3841 cacgctcggc ggaagcttgg ctttcatctg ataggccagc ttccttacca gcgacgcatg
     3901 tttctgcagc aaatcattct tgtctgcctt accgttgaag ttatacatgg ccacatcatg
     3961 ctccgagacc tacgctatcg gttgccatac cgtatgtgcc cttagtgaga tgcaatagcg
     4021 agaactgcct ggccagacgt ttgaacgcaa tggcggcgcc ggtcagcggg aacgcctcca
     4081 atacagtacg tccttgcccc gatgcgcgcc ggatatgctc atcggccggg atcgccccca
     4141 gaaaattcag ttgcactgcc agataacggt tggcgacctg cgccatattc gcgaagacca
     4201 ttttcgcctc cgcatccgat ccgccggtga ccagcagact gaaaggacgg cgacccaggc
     4261 ggtcgttcaa gcttttgatc agcgcatacg ctgccttgat agaacccaga ctagtcgaca
     4321 tatgcacgac gatctcacca cccatttcca tacccggcac cggcagcgac ttatcttcat
     4381 ttagctcgcc attgacgagc gtgacgtcgg attcatcagt caggacatca aaaatttccg
     4441 ccagacggga cgcctgcaga tgcgtggcta cagagacact tttgtgtgac agacgcgcca
     4501 gcacgaaacc ttccggcacc cgccgcagcg cctcatccag aacacgctcc tgacacgcca
     4561 catcgagcag gctggcgaca gcgcgaccgc caaaacggga cagtacgctc gatggcgtca
     4621 tacaggcgtc aaccagcaaa acactgcaat catgagcagc aagagaggct gccagattga
     4681 tcaacatcgc atctttttcg gcgtctggca gcaccgagaa gaagctgaac acgctcggcg
     4741 tcggacgctc tagcatccgg cgcaatcctt ccgcctgatc agcatcatag ctagccaagg
     4801 atcacctccc gcaggctttt gtcgtgcatt gccattgctg ttggtccaac cacaaccggc
     4861 aactcgaaat cctgaaactc gaacggcgcc gtttcacgtt tcagtttaaa agcacggtcg
     4921 gctaaataca ggttattggc aacatgcagg tcttccggca cgcgctgacc attggctaca
     4981 tagtacagat tgagcttctg acgaatgact acatcgagta cattgccgat ggtggcagct
     5041 tcatccagct tggtaataat gcaacccgcc agaccatcgc ccgaataggc actcacgact
     5101 tcattgagcg tgctgccgat tgcagttgca ttcagacaca gcagacgttt gattttcgcg
     5161 cctgcgccgg atagcatggc ggcctgctcg gcgaccatct gatcgcgctg gcccataccg
     5221 gcggtgtcga taaggacagt atgtttacca tacagttcat ccagggaatg cgcaaatcag
     5281 cctcgtcttt caccgaatgc accatgacac caaggatatt gccgtagatg cgcaactgtt
     5341 cataaccacc gacacgatag ccatcggtag tgatcaacgc cagcttgcca gggccatgct
     5401 gcatcacgca acgcgctgcc agtttggcgg tagtggtggt cttgccaacg ccggtcgggc
     5461 cgaccagcgc atagatgcca ccttcctcca gcaattcatt ttcgctggac atggtcacca
     5521 gattcctggc cagcaccgac ttgacccaga acatcgtgtc ctgtgatttg ggattctgcg
     5581 gcaggttttc ggtaatcagg cgcgacaggc tggcactaaa acctgctgcc aacatttcgc
     5641 gcatgaccgc agctttgagt ggttcgcgtt tttgtgtacc gccccaggaa atctctgcca
     5701 gttgtgtttc cagtacaccg cgtatggaat ggatttccat catcacacca gacaggattt
     5761 catcataatc cttaccatcg aacacagcac ccgatcgttt cggaccttga gcgcccgacg
     5821 cagcaagttt gagatttgtt tgcggctcct tggtgcgaac agccttg
//
LOCUS       DASRDH010000163         3463 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_960591,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000163 DASRDH010000000
VERSION     DASRDH010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 3463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3463
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_960591"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="VFA14_03735"
     CDS             <1..534
                     /locus_tag="VFA14_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZS80526.1"
                     /translation="VIDEAYNEYLSADLQYESAGWVRCHPNLIVSRTMSKAYGLAGLR
                     IGFAIAQPAVTDLLNRIRQPFNVNSLAQAAAVAALNDKAFLQKSAQLNAQGYQQLTAA
                     FDDIGLRYVPSCGNFVLVRVGEDDGAGARVNLALLRKGIIVRPVGNYGLLQWLRVTIG
                     LPEENAAFITALKAILG"
     gene            534..1436
                     /locus_tag="VFA14_03740"
     CDS             534..1436
                     /locus_tag="VFA14_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HZS80527.1"
                     /translation="MIVKKIVIFGVGLIGGSFALALKRAGVAGQIVGVDRSEAPLLRA
                     KALGIIDVLSTSVADAVQGADLIMLAAPVAQTGSILAGIAPHLQTGTVVTDVGSTKAD
                     VVAAAREALGGKIAQFVPGHPIAGREQSGPEAALADLYVGKKFVMTPLPENAEGDIER
                     VVQAWQACGALIHRLSPQEHDAVFAAVSHLPHLLAYALVDDIAAKPHASVLFQYAASG
                     FRDFTRIAGSSPEMWRDISLSNRTALLRELDVYLAQLMRMRKMLVERDSDGLEKIYVH
                     ARQARCNWAGEIETAGWQSKESGD"
     gene            1514..2845
                     /gene="aroA"
                     /locus_tag="VFA14_03745"
     CDS             1514..2845
                     /gene="aroA"
                     /locus_tag="VFA14_03745"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008331554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HZS80528.1"
                     /translation="MKQVSYPHHIDFHPVAHAQGTVKLPGSKSISNRVLLLAALSRGT
                     TRIMDLLASDDTLVMLMALQQLGVKWEQVDGTQDYIVHGVDGVLPVHQADLFMGNAGT
                     AIRPLTAVLAVLGGDYILHGVPRMHERPIGDLVDALNNIGMRIEYTGNPGYPPLHIRR
                     GVLGAQRMKVRGNVSSQFLTALLMAAPLVVKSGLMVIEVEGELISRPYIEITLNLMRR
                     FGVEVQCDGWQSFTIAAGQYYRSPDVIHVEGDASSASYFLAAGTIAGGPVRVEGVGKD
                     SIQGDVRFVEALRQMGASITMGDNWIEASAHGVLKAIDADFNHIPDAAMTIAVAALYA
                     NGTSVLRNIASWRVKETDRLAAMATELRKLGAIVEEGADYLSVTPPAQLTPAAIDTYD
                     DHRMAMCFALVSLDGAARRGVRVRIKDPKCVAKTFPDYFRVFQQIAEQTLV"
     gene            2852..>3463
                     /gene="cmk"
                     /locus_tag="VFA14_03750"
     CDS             2852..>3463
                     /gene="cmk"
                     /locus_tag="VFA14_03750"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008446210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HZS80529.1"
                     /translation="MTAAAIPVITIDGPTASGKGTVAQKVAQHLGFHYLDSGALYRLT
                     ALSAMRRGVDMSDEHALAKSAVHLRCRFDGAHIFLANEDVTQAIRTEEVGNTASKIAL
                     LTMVRQALYGRQLSFRKAPGLVADGRDMGTVIFPYAPLKVFLTASVEARAQRRYKQLI
                     DKGFSASISGILKDLRERDARDMHRASAPLKQAEGAYLLDTSVM"
BASE COUNT          681 a          918 c         1086 g          778 t
ORIGIN      
        1 gtcatcgacg aagcttataa cgaatacctg tctgccgatc tgcaatacga gtcagccggt
       61 tgggtacgct gccatccaaa cttgatcgta tcgcgcacca tgtccaaggc ttacggcctg
      121 gcaggtctgc gcatcggttt cgcgattgcg cagccggccg tcaccgacct gctcaatcgt
      181 atccgtcagc cgttcaacgt caattcgctg gcgcaggccg ctgccgttgc tgcactgaac
      241 gacaaggcat tcctgcaaaa gagtgcgcag ctcaacgcgc aaggttatca gcaactgact
      301 gctgcttttg acgacatcgg tctgcgctat gtgccgtcct gcggcaattt cgtgctggtg
      361 agggtcggtg aagacgacgg cgccggcgcg cgcgtgaatc tggcattgct cagaaaaggc
      421 atcatcgtgc gtccggtcgg taattacggt ttgctgcaat ggttgcgcgt cacgatcggc
      481 ttgccggaag aaaatgcagc cttcatcaca gcattgaaag cgatattggg ttgatgatcg
      541 tgaaaaaaat tgtcatcttt ggtgttggtc tgatcggcgg ttcatttgcg ctggcattga
      601 aaagagccgg cgtggccggg cagatcgtcg gcgtcgaccg ctcggaagca ccgttgttgc
      661 gggcaaaagc attgggcatc atcgatgtcc tcagtacctc ggttgccgat gccgtgcaag
      721 gcgcagattt gatcatgctg gcagcgccgg tggcgcaaac gggatctatt ctggctggca
      781 tcgcgccgca cttgcaaacg ggtactgtcg tcacggacgt cggcagtacc aaggcggatg
      841 tggtggcggc cgcgcgcgaa gcgctgggtg gaaaaatcgc gcagttcgtg ccggggcatc
      901 cgatcgcggg gcgcgagcag agcggaccgg aagcggcgct ggcggatctg tatgtcggca
      961 aaaagtttgt catgacgcct ttgccggaaa atgccgaggg cgatatcgag cgtgtggtgc
     1021 aagcctggca agcctgcggt gcgctgattc atcgactgag tccgcaggaa catgacgccg
     1081 tgtttgccgc ggtcagtcat ttgccgcatc tgctggctta tgcgctggtc gacgatattg
     1141 ctgcgaagcc acatgcgtct gtcctgtttc agtatgcggc cagtggtttc cgggatttca
     1201 cgcgcattgc tggatcgtcg ccagaaatgt ggcgtgatat ttctctttcc aatcgtactg
     1261 ccttgctgcg cgagcttgac gtttatctgg cccagttgat gcgcatgcgc aagatgttgg
     1321 tcgagcgcga tagcgatggt ctggaaaaaa tttacgtcca tgcccggcag gcgcgatgca
     1381 actgggcggg cgaaattgaa accgccgggt ggcaaagtaa agagagcggg gattgagcag
     1441 gaattgccgg tctgacgcgc aggcgctgtc agagcgtcaa cacgttctat aacccattga
     1501 atgagtattt ccgatgaaac aggtttctta tcctcatcac attgacttcc atcccgtggc
     1561 gcatgcgcag ggtacggtca aactgcccgg ctccaaaagc atttccaatc gtgttctact
     1621 gttggcggcc ttgtcgcgtg gtacgacccg gatcatggat ctgttggcct ccgacgatac
     1681 cctggtgatg ctgatggcct tgcagcagct cggtgtcaaa tgggagcagg tcgacggcac
     1741 gcaggattac atcgtgcatg gcgtggatgg cgtactgccg gtgcatcagg ccgatttgtt
     1801 catgggtaac gccggcactg cgatccgtcc attgacggca gtgttggcgg tgcttggcgg
     1861 cgactatatc ctgcatggtg tgccgcgtat gcatgagcga ccgatcggtg atctggtcga
     1921 tgcgctcaac aacatcggca tgcgcatcga atacaccggc aatccgggtt acccgccgct
     1981 gcatatccgc cgcggcgtac tcggtgcgca gcgcatgaaa gtgcgtggca atgtatccag
     2041 ccagttcctg acggccctgc tcatggctgc gccgctggta gtgaagtccg gactgatggt
     2101 catcgaggtc gagggtgagt tgatttcccg gccttacatt gaaattacct tgaatctgat
     2161 gcgccgtttc ggcgtcgagg tgcaatgcga tggctggcag tcgtttacga ttgccgccgg
     2221 ccagtattat cgcagcccgg atgtcatcca tgtcgaggga gatgcttcgt cggcgtcgta
     2281 tttcctcgcc gccggcacaa tcgcgggcgg accggtccgc gtggaaggcg tcggcaagga
     2341 tagcattcag ggcgatgtgc gttttgtcga agcactgcgc cagatgggcg ccagcatcac
     2401 catgggtgac aactggatcg aggcttctgc gcacggcgta ctcaaggcca tcgatgctga
     2461 cttcaaccat attcctgatg cggccatgac gattgccgtg gcagctttgt atgccaatgg
     2521 caccagcgta ctgcgcaaca tcgccagttg gcgcgtcaag gaaaccgacc gcctggcggc
     2581 catggcgacc gagctacgca agctcggcgc catcgtggaa gaaggcgcgg attatctgag
     2641 cgtgacgccg ccggcgcagt tgacgccggc tgctatcgat acctatgacg atcatcgcat
     2701 ggcgatgtgt ttcgcgctgg tatcgctgga cggcgcggcg cggcgcggcg tgcgcgtgcg
     2761 catcaaggat ccgaagtgcg tggccaagac ttttccggat tacttccggg tattccagca
     2821 gatcgctgaa cagacattgg tttgaattcc catgactgcg gcagctattc ccgttatcac
     2881 catcgacggc ccgactgctt cggggaaagg cacggtggcg caaaaggtgg cgcagcatct
     2941 cggtttccat tacctggatt caggcgctct gtatcggttg acggccttgt cggcgatgcg
     3001 tcgcggcgtg gacatgagcg acgaacatgc gctggccaag agtgccgtgc atttgcgctg
     3061 tcgtttcgac ggtgcgcata tttttctggc caatgaagat gttacgcagg ccattcgcac
     3121 ggaagaggtc ggtaacacag catcaaaaat tgcgctactg acaatggtcc ggcaagcctt
     3181 gtatggcagg cagctcagtt ttcgcaaagc acctgggctg gttgctgatg gtcgcgacat
     3241 gggtacggta atcttcccgt atgcaccgct gaaggtgttt ttgactgcca gtgttgaagc
     3301 aagagcgcaa agacgttata agcaattgat tgacaagggg ttttctgcta gcataagcgg
     3361 cattttgaaa gatttgcggg aacgtgacgc gcgcgacatg catcgcgcat cggcgccgct
     3421 caagcaggcg gaaggggcat atctgctgga tacttccgtc atg
//
LOCUS       DASRDH010000164         3574 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1491703,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000164 DASRDH010000000
VERSION     DASRDH010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 3574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3574
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1491703"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /gene="flgJ"
                     /locus_tag="VFA14_03755"
     CDS             <1..384
                     /gene="flgJ"
                     /locus_tag="VFA14_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013234027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HZS80530.1"
                     /translation="KFMLAQAALETGWGKREIRTRDGSSAHNLFGIKATGNWTGRVTE
                     ATTIEYINGVPQRRVEKFRAYDSYAEAFRDYANFLKNNPRYEKVLVNAQDADGFALGL
                     QRAGYATDPHYAAKLSSIIRKSFSA"
     gene            483..2753
                     /gene="flgK"
                     /locus_tag="VFA14_03760"
     CDS             483..2753
                     /gene="flgK"
                     /locus_tag="VFA14_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007882200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HZS80531.1"
                     /translation="MATTNIFSIGQSALAAAQIAQATTSHNISNATTPGYNRQVVVQS
                     TLGSMKYGYVYGGYGFIGQGTQVTQIKRIYDDFLNKQLLSSQTSVSSLDAYYAQIGQI
                     DDLVADTSAGLSPSLQDFFKSIQNLSATPNNDGVRQSVLSAGQMLASRFQSLNSQLAQ
                     HRASLNSQITFGVTTINAYARQIQELNDAVLKTSGPNGVVSNDLLDQRDQVIADLNKI
                     IKVTTLPQDSGALSVFIGTGQPLVLGNVASTLVATPASDDPVRLQISYQNNGQNVVIP
                     DSAFTGGSLGGILQYRSDTLDLVQNSLGRIAIAIGMEFNAQHRLGQDQNGNLGTDFFE
                     IATPVIYSDRKNSTTAVATATITDPSRLTTSDYRLTYDGAKYIFLRLSDGNKTVVDPT
                     APGAFPVTIDGVTYATPTMVAGDSFLIKPTVAGANLINVVITNTQRVAAGIPVLTSIN
                     AVTNMGIVTTGNTGSGVVTSATVDKSAFTPDSTQTYTYDAGMLTLTPPAAVTVTTTAG
                     VTTTYAAGAAIPYSTGAVISSGGVRFVLSGTPANGDTYKFSPVPANTGTATISPGKID
                     SSYTPLAAGTRFAFTFDSANSQFTPSASVGPVVVTHADGSPPTSYAAGAPIDYVAGDT
                     FSTAGASFTITGQPANGDQFYLSPNIGGISDNRNAQALSALQTANTIGNTSFQGAYSQ
                     LVAVIGSKTNEIDVTNKAELARLNSIQEQQQSGSGVNQDEELSNMIKNQTAYQAAAKI
                     IQAASDMLNILLTLGD"
     gene            2785..>3574
                     /gene="flgL"
                     /locus_tag="VFA14_03765"
     CDS             2785..>3574
                     /gene="flgL"
                     /locus_tag="VFA14_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008328160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HZS80532.1"
                     /translation="MRISTNMLYKLSDTGLLSKQESIVKLRQQINAGQKVMTPSDDPI
                     AASRALDLSQTQGLNTQYRTNRNNANSSLGAVSAALSRAMAIMTQIKADVIRAGDGSL
                     SNAERNYMAIALQGNLEEFLGLANTTDGMGGYVFAGFKSATQPFTLDAMGNIVYNGDQ
                     GEQTLQVDATRQIEVSVSGQAVFQGNGQDTFRMLQNLITVLSTPTTEAGDNADVVAAD
                     TFEYPSGSGQYPIAAYKAAQAALDEAEPTDPNYAALMTQTAKAKL"
BASE COUNT          851 a          978 c          987 g          758 t
ORIGIN      
        1 aaattcatgc tggcgcaggc agcgttggag acaggctggg gcaagagaga aattcgtact
       61 cgggatggca gttcggcaca caatctgttc ggcatcaagg ctacgggcaa ttggactggc
      121 agggtgacgg aagcgaccac gatcgaatat atcaacggtg tgccgcaacg ccgggtggaa
      181 aaattccgcg cttatgactc ttacgctgag gcattccgtg actacgccaa ttttctgaaa
      241 aacaatccgc gctacgaaaa agtgttggtt aacgcccagg atgcggatgg ttttgccctc
      301 ggcttgcaac gggcaggtta tgcaactgat ccgcattatg cggcaaaatt atcgagcatc
      361 attcgtaaat cgttttcggc ttgatcagtc ggtaggttca tgagaaccgc ttcctggacc
      421 gttccctaaa gttttgctgc cagatgccgt tgactaagat attccgttaa agagaatacg
      481 acatggctac taccaatatc ttcagtatcg ggcaaagcgc gctcgctgca gcgcagatag
      541 cgcaggcgac gactagccat aatatttcca atgcgacgac cccgggctat aaccggcagg
      601 tggtggtaca gagtacgctt ggcagtatga aatacgggta tgtctatggc ggttatggtt
      661 ttattggcca gggcacgcag gttacgcaaa tcaagcgtat ctatgacgat tttctgaaca
      721 agcagctgtt gtcgtcccag acgagcgtta gctcgttgga tgcttactac gcgcagatcg
      781 gccagatcga tgatctggtc gcggatacgt cggcgggttt gtcgcccagc ttgcaggatt
      841 tcttcaaaag catacaaaac ctgtcggcga cgccgaacaa cgatggcgtg cgtcagtcag
      901 tgctttcggc tgggcagatg ctggcgtcac gcttccagag cctcaacagt cagttggcgc
      961 aacaccgcgc cagcctcaat agccagatta cctttggcgt tacgacgatc aacgcctatg
     1021 cccgccagat tcaggaactc aacgatgcgg tcctgaaaac atctggcccc aacggtgtgg
     1081 tttccaacga tttgctggat caacgcgacc aggtcatcgc tgacctcaac aaaatcatca
     1141 aggtcaccac gttaccgcaa gacagcggcg cgctgagtgt gtttatcgga actggccagc
     1201 cgctggtgct gggcaacgtc gcttcgacgc tagtggcgac tccagcgtcc gacgatccgg
     1261 tacgcctgca gatttcttat cagaacaatg gtcaaaacgt tgtgatcccg gattcagcat
     1321 tcaccggtgg cagtcttggc ggcatcctgc aataccgtag cgacacgctg gacctggtgc
     1381 aaaactcact cggccgtatc gctatcgcca tagggatgga attcaatgcc cagcacaggc
     1441 tgggtcagga tcagaatggc aacctcggta ccgatttttt tgagatcgcc acgccggtga
     1501 tttatagcga cagaaaaaat tcgacgactg ctgtggcgac cgcgaccatt acggatccct
     1561 ccagactcac gactagcgat tacaggctga cctacgacgg cgccaaatat atttttctcc
     1621 gtttgtctga cggcaacaag accgttgtcg accccacggc tcctggcgcg ttccctgtga
     1681 caattgacgg tgtgacctat gcaacaccga ccatggtcgc aggcgacagc tttctgatca
     1741 agccgaccgt ggccggtgcc aatctgatca acgtggtgat caccaacacg cagcgggtgg
     1801 ccgcaggcat accggtcttg acatccatca atgcggtgac taacatgggt atcgtcacga
     1861 ccggcaatac cggtagcggc gtggttacct cggcgacggt cgataaatcg gcatttacac
     1921 cggactccac ccagacttat acctatgatg ccggcatgct gaccctgacg ccaccggcag
     1981 cagtgacagt gaccaccact gctggcgtga cgacgacgta tgcggccggc gcagcgattc
     2041 cttattcaac tggcgcggtc atttccagcg gtggcgtccg ctttgtgctg agtggtacgc
     2101 cagccaatgg cgatacctac aaattttccc cggttcctgc caataccgga acggcgacca
     2161 tttcaccggg taagatcgac tcgagctata ccccgctggc agcaggcacc cggtttgctt
     2221 tcacctttga cagcgccaac agccagttca cgccatcggc atcggtcggt cctgtggtgg
     2281 tgacgcatgc tgatggttcc ccgcccacca gctatgccgc cggcgcgccg atcgattatg
     2341 tggccggcga tacatttagt acggctggcg cgagcttcac gatcactggt cagccggcca
     2401 atggcgatca gttttatctc agcccgaata tcggcggcat cagcgacaat cgcaacgcac
     2461 aggccctgag tgccttacaa acggccaata ccattggcaa cacttcgttc cagggagcat
     2521 attcgcagct ggtcgctgtc atcggcagca agactaatga aatcgacgtc accaacaagg
     2581 ctgaacttgc gcgcctgaac tcgattcaag aacagcagca atccggatcc ggggtcaacc
     2641 aggatgagga attatccaat atgatcaaaa atcagacggc ttatcaggca gcagccaaaa
     2701 tcattcaggc tgctagcgat atgctgaata tcctgcttac gcttggcgat tgaattgttg
     2761 gtcaacccct gaaggaaaat taacatgcgt atcagcacca acatgctcta caaattgagc
     2821 gatacaggtc tgctcagcaa gcaggaatcg atagtgaagc tcaggcagca gatcaacgct
     2881 ggtcaaaagg tgatgacgcc atcggatgat ccgattgcgg cgtcgcgcgc cctggatctg
     2941 tcgcagacgc aggggttgaa tacgcagtat agaactaacc gcaataatgc caacagttcg
     3001 ctgggggcgg tgagcgctgc gctcagcaga gccatggcga tcatgaccca gatcaaagcg
     3061 gacgtcatca gggccggtga tggctcgctt tccaatgccg agcgaaacta tatggcgatt
     3121 gccctacagg gcaatcttga ggaatttttg ggtttggcca atacgaccga tggtatgggt
     3181 ggttacgtgt tcgccggttt caagagtgcg acgcaaccgt ttacattgga tgcgatgggt
     3241 aacatcgtct acaacggcga tcagggcgag caaacgctgc aggtcgatgc gacgcgccag
     3301 atagaagtca gcgtatccgg ccaggccgtg ttccagggaa atggtcagga tacgttcagg
     3361 atgctccaga atctgatcac cgtgctgtcg acgccgacga ccgaagccgg cgacaacgca
     3421 gatgtggtgg cggccgatac ctttgaatat ccatccgggt caggccagta cccgattgct
     3481 gcttacaagg cagcgcaagc tgcgctggat gaggcagagc cgaccgatcc caactatgcg
     3541 gcgctgatga cgcagactgc caaggccaag ttat
//
LOCUS       DASRDH010000165         2094 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_828904,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000165 DASRDH010000000
VERSION     DASRDH010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_828904"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2094)
                     /gene="mutS"
                     /locus_tag="VFA14_03770"
     CDS             complement(<1..>2094)
                     /gene="mutS"
                     /locus_tag="VFA14_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HZS80533.1"
                     /translation="ERIAPAETLIGDTEEALHLHLSGGKLTTVPDWHFDRSEKALLDQ
                     LSVATLDGFGAQGMHAAIGAGSALLRYAQSTQGKDLQHVRTLTVESENEFIGLDAATR
                     RNLELTETIRAQDAGALTPTLFSLLDHCRTAMGSRLLRYWLHHARRDQRIAQTRHAAI
                     NALMRAAACSGLSATLATVPDIERITTRIALLSARPRDLSGLRAGLQQLGALRKYVSM
                     CSRDADAPLLQELHDALATPSECVDLLERAIMPEPAAMVRDGGVIARGFDAGLDELRG
                     LSENAGQFLIDLEARERSRTGIANLRVEYNKVHGFYIEVTHGQSGKVPDDYRRRQTLK
                     NAERYITPELKAFEDKALSAQERALSREKFLYDQLLQDLAPHIVTLQAIAHALAQLDT
                     LTALADHAARNDWCAPQLTTEPVILIEQGRHPVVENQIEHFIANDCAFSAERRLLLIT
                     GPNMGGKSTFMRQVALITLLAYIGSFVPAASATIGPIDRIFTRIGAADDLAGGRSTFM
                     VEMTESAAILNNATENSLVLMDEVGRGTSTFDGLALAWAIARHLIDVTRSFTLFATHY
                     FELTQLPEIHPTASNVHLSAIEHGDSIVFLHTVQAGPASQSYGLQVAQLAGIPTPVIR
                     AARKRLAILEKQSAQATPQFDLFDTPPDQEREARIIQQHAPAMNPSPADPATQAVVDA
                     LAAIDPDALTPRQALD"
BASE COUNT          364 a          624 c          688 g          418 t
ORIGIN      
        1 atccagcgcc tgcctcggcg tcagcgcgtc cggatcgatc gcggccagcg catctacgac
       61 cgcctgtgtt gccggatcgg cgggagaggg gttcatggcg ggtgcgtgct gctggatgat
      121 ccgggcttcc ctttcctgat cgggcggtgt gtcgaacaga tcgaactgcg gcgttgcctg
      181 tgccgactgt ttttccagta tcgccaggcg cttgcgcgcc gcgcggatga ccggcgtcgg
      241 tatacccgcc agttgcgcta cctgcaaacc gtagctttgc gaggccgggc cggcctgcac
      301 ggtatgcagg aagacgatgc tgtcgccgtg ttcgatggcg gataaatgga cgttggacgc
      361 ggtgggatga atttccggca gttgcgtcag ctcaaaataa tgagtcgcaa acagcgtgaa
      421 actgcgcgta acatcgatca gatgcctggc gatcgcccac gccagcgcaa ggccgtcgaa
      481 ggtcgaggtg ccgcgcccga cttcatccat cagcaccagc gaattttcgg tagcgttgtt
      541 gagaatggcg gccgattctg tcatctctac catgaaggtg gagcggccgc cggccagatc
      601 gtcggccgcg ccgatgcgcg tgaagatacg gtcgatcgga ccgatcgtcg cgctggcggc
      661 tggcacgaaa ctgccgatgt aagccagcag tgtgatcagc gccacctgac gcatgaaggt
      721 cgattttccc cccatattgg gaccggtgat cagcagcagc ctgcgctcgg cgctgaaagc
      781 acagtcgttg gcaatgaaat gttcgatctg gttttccact actggatgac ggccctgctc
      841 gatcaggatg accggctctg tcgtcagttg tggcgcgcac cagtcgttac gcgcggcgtg
      901 gtcggccagc gccgtcagcg tatcgagctg ggccagcgcg tgggcaatgg cttgcagcgt
      961 gacgatgtgc ggcgccagat cctgcagtaa ctggtcgtag aggaatttct cgcgcgacag
     1021 ggcgcgttcc tgcgccgaca gcgccttgtc ttcaaaggct ttcagttcgg gcgtgatgta
     1081 acgctcggcg ttcttgagcg tttgacggcg ccggtagtcg tccggcacct tgccgctctg
     1141 accatgcgtg acttcgatgt aaaaaccatg caccttgttg tactctaccc gcagattggc
     1201 gatgccagta cgggagcgtt cgcgggcctc cagatcgatc agaaactggc cggcattttc
     1261 cgacaggccg cgcagttcat caagaccggc gtcgaagccg cgcgcgatta cgccgccgtc
     1321 gcgcaccatc gccgccggtt ccggcatgat ggcgcgttcc agtaaatcga cgcattcgct
     1381 tggtgtcgcc agcgcgtcgt gcagctcctg cagtaatggc gcgtcggcat cacggctgca
     1441 catcgagaca tacttgcgca acgccccgag ctgttgcaaa ccggcgcgca ggccgctcag
     1501 gtcgcgcgga cgcgccgaca gcagcgcgat gcgtgtcgtg atgcgttcga tatcggggac
     1561 ggttgccagc gtggccgaca ggcccgagca ggcggcggcg cgcatcaacg cgttgatggc
     1621 agcgtggcgg gtttgggcga tgcgctgatc gcggcgcgca tgatgcagcc agtagcgcag
     1681 caggcgtgac cccatcgctg tgcggcaatg gtcgagcagc gagaacagtg tcggcgttag
     1741 cgcgccggcg tcttgagcgc ggatggtttc cgtcaactcc agattacggc gtgttgcggc
     1801 atcgaggccg atgaattcat tttctgactc caccgtcagc gtgcgcacat gttgcagatc
     1861 cttgccctgg gtggattgcg catagcgcag cagtgcgcta ccggcgccga tggcggcatg
     1921 catgccttgc gcgccgaaac cgtccagcgt tgccactgac aattgatcca gcaaagcctt
     1981 ttcgctgcgg tcgaaatgcc agtccggcac cgtggtcagt ttgccgccgg acagatggag
     2041 atgaagcgcc tcctcggtat caccgatgag cgtttcagcg ggtgcaatgc gctc
//
LOCUS       DASRDH010000166         5380 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_340841,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000166 DASRDH010000000
VERSION     DASRDH010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 5380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5380
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340841"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            9..89
                     /locus_tag="VFA14_03775"
                     /pseudo
     CDS             9..89
                     /locus_tag="VFA14_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003261195.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
     gene            320..892
                     /locus_tag="VFA14_03780"
     CDS             320..892
                     /locus_tag="VFA14_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HZS80534.1"
                     /translation="MKENLVRIALLLLFPLFLLAACDRSIDERLAAKAAAEKSLGISP
                     STFAANFDGAIRDVLEDRKNGDVDRLAPLYRIDASQLEKHTLRAEVGPSQTAILGSLA
                     KNGDLKSVGVMLSSPGEGARAEFLLCAEAASRLLTTGGDEKKLPDMLNRLLTNALNNP
                     GERMTEIVDDKLLSVEILKQGVMFQIEHAQ"
     gene            986..1921
                     /gene="lpxC"
                     /locus_tag="VFA14_03785"
     CDS             986..1921
                     /gene="lpxC"
                     /locus_tag="VFA14_03785"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HZS80535.1"
                     /translation="MLKQCTIKTSVRTVGIGLHSGAKVELTLRPATVDNGIIFRRVDL
                     DPVVSFPASANGVGDTRMASVLSMNGARVSTVEHLLSACAGLGIDNLYVDLTAEEIPI
                     MDGSASSFVFLLQQAGLQEQNAPKQFIRVKKTVEVREGQGAKEKWARLEPYNGFKLKF
                     FIEFNHPAVDSTGQTAEVDFGIASYVKEIARARTFGFMQDVETLRGMGLARGGSLENV
                     IVMDEYCILNADGLRYHNEFVRHKILDAIGDLYLIGHPLLAAYSAHKSGHGLNNQLLR
                     ALLAQPDAYEIVTFDTLESAPQTYVMQAEQEWALT"
     gene            complement(1947..2453)
                     /locus_tag="VFA14_03790"
     CDS             complement(1947..2453)
                     /locus_tag="VFA14_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007878101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HZS80536.1"
                     /translation="MKYPSTFTPYRQGPSAAQLKRKPRDVTDFLQLDNVMAGLLPAIT
                     RMTALQKSCAAILPAMFKSCDAVNVEGEQLLLSVPNAALAAKLKQQLPKLQEHLSKDG
                     WQIDTIRIKVQVGRNDVVPVARRKPTLPSLAVASFAKLNDTLEDSPRNQALKMALAGM
                     MRRNSGGR"
     gene            2632..>5380
                     /gene="secA"
                     /locus_tag="VFA14_03795"
     CDS             2632..>5380
                     /gene="secA"
                     /locus_tag="VFA14_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008116400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HZS80537.1"
                     /translation="MSLLTQIFGSRNQRLLKQYQKTVYRINALEADIERLPDADLQAK
                     TPEFKQRIASGVRLDDLLPEAFAVCREASRRVLRMRHFDVQLIGGMVLHFGKTAEMRT
                     GEGKTLMATLPAYLNALAGKGVHIVTVNDYLAQRDAEWMGKLYGWLGLSTGINLSQME
                     HDLKQSAYAADITYGTNNEFGFDYLRDNMVFDAGQRVQRGLHFAIVDEVDSILIDEAR
                     TPLIISGQDENHTDLYHKIDAVPPLLMQQIGEETPDGKGRIEVPGDYTKDEKSHQVLL
                     TEAGHEKAEQILTSMGLLPEGASLYDASNISLVHHLYAALRAHTLYHKDQQYVVQNGN
                     IVIVDEFTGRLMTGRRWSDGLHQAVEAKEKVKIQNENSTLASITFQNYFRMYGKLSGM
                     TGTADTEAYEFQEIYHLETVVIPPNRPSARKDRQDQVYKSAQEKYAAMLKDIQDCHER
                     GQPVLVGTTSIENSELLSGILKQAGLPHNVLNAKQHAREAEIIAQAGRPRMITIATNM
                     AGRGTDIVLGGNVEKQIQIIDAALPDAAAQAQKLRDEWQALHDHVVGAGGLHIIGTER
                     HESRRVDNQLRGRSGRQGDPGSSRFYLSLDDALLRIFAGDRVRAIMERLKMPEGEPIE
                     AGIVTRSIESAQRKVEARNFDIRKQLLEYDDVANDQRKVIYQQRNALLEASDMSEMIT
                     SLRDGVFTELFRTHVPEESIEEQWDIPALEAALGNEWQLAVPLSAMLEAEPNLTDKEL
                     LERVLSAAEVAHDAKARVVGKEAFNSFERSVMLQSIDSHWREHLAALDHLRQGIHLRG
                     YAQKNPKQEYKREAFELFAGMLDLIRNEVIKIVMTVRIENREAIQEAEEHMAQSHVGN
                     VHYQHADFDADTAPEELLAPMTHAGAGGKAAQLPVNGVPKVGRNDPCPCGSGKKYKQC
                     HG"
BASE COUNT         1251 a         1532 c         1510 g         1087 t
ORIGIN      
        1 tcacttaacg ctgggcgggc acgccgttga acgagcggca ggtaaaactg ctcaaccgtc
       61 tgctcgacgg cttcgggggc aagctcacca tgctctgtgt caacttatat ccggtgctga
      121 caccttgcca cgccgggtaa atgtatttat gctcttctgt attccgcgtt acttgagact
      181 ttatcagaag atgccgcacg gcaaatagag agccgctgac aaaacgccgt tggctgcact
      241 gcgcctccgc atctttgtac tgtcttcgtc ggcgcgcctt gccaccgatg ctccgttagc
      301 ggtcctaaag gaggagtaga tgaaagaaaa cctcgtccgc atcgcacttt tattgctttt
      361 tcccctgttc ctgttagccg catgcgaccg ttccatcgat gaaagactgg cggcaaaagc
      421 agccgctgaa aaatcgctgg gcatctcgcc gagtacgttc gcggccaact tcgatggtgc
      481 aatccgtgat gtgctggaag atcgcaagaa tggagatgtc gaccgcctgg cgccgctcta
      541 tcgcatcgac gcctcacaac tggaaaaaca tactctccgg gcggaggtcg gtccgtcgca
      601 gacagccata ctgggcagtc tggccaagaa tggcgatttg aaaagcgtcg gtgtcatgtt
      661 gagcagtcct ggcgaaggcg cgcgcgccga atttctgctg tgcgcagaag cggccagccg
      721 gctgctgacc accggcggtg acgaaaaaaa actgccggat atgctcaatc gtctgctcac
      781 caatgctctc aacaaccccg gtgagcgcat gacggaaatc gttgatgaca aattgctatc
      841 agtggaaatc ctcaagcaag gagtgatgtt ccagatcgaa cacgcgcaat gaaccgcccc
      901 caggagcctg ttcaggatcc cggcggagat tctgaacagg ttccaagaag cctgaggatc
      961 cgatcggtgg gctataatta gcagcatgtt gaaacaatgc actatcaaaa cttccgtccg
     1021 gaccgtcggc atcggcctgc actccggcgc caaggtcgag ttgaccctgc gtccggcaac
     1081 cgtcgataac ggcatcatct ttcgccgcgt cgacctcgac cctgtcgtga gctttcctgc
     1141 gtcggcaaat ggtgtcggcg atacccgcat ggcatcagtg ctgtccatga acggtgcgcg
     1201 cgtttctacc gtcgagcatc tgctgtccgc ctgtgccggg cttggcatcg acaatttata
     1261 tgtcgatctg accgcggaag aaattccgat catggacggc tcggcatcat ctttcgtttt
     1321 cctgttgcaa caagcaggtc tgcaagagca gaacgcgccg aagcagttca tccgcgtcaa
     1381 aaaaaccgtc gaggtgcgcg aaggccaggg cgccaaggag aaatgggcgc gcctggaacc
     1441 gtacaacggc ttcaaactga aattcttcat cgaattcaat catcccgccg tggacagcac
     1501 cggccagacg gccgaggttg atttcggcat tgcatcctat gtcaaggaaa ttgcccgtgc
     1561 gcgcacgttc ggtttcatgc aggatgtcga gaccctgcgc ggcatggggt tggcgcgcgg
     1621 cggctcgctc gagaacgtga tcgtgatgga tgaatactgc atcctcaacg ccgatggcct
     1681 gcgttaccac aatgagttcg tgcgccacaa gattctcgac gccatcggcg acctgtacct
     1741 gatcggccat ccgctactgg ctgcttacag cgcgcataaa tccggtcatg gcctcaacaa
     1801 tcagctactg cgcgcgttgc tggcgcagcc cgacgcctat gagatcgtca cattcgatac
     1861 gctggagtcg gcgccacaga cctatgtgat gcaggccgaa caagagtggg cgctaactta
     1921 gaacattaga acgcgttcca ggctttctat ctgccgccgc tgttgcgacg catcatgccc
     1981 gccaatgcca tcttcaaggc ctgattgcgc ggcgagtctt cgagtgtgtc gttaagtttt
     2041 gcgaatgacg ccactgccag gctgggcaaa gtcggtttcc ggcgcgcgac tggaacgacg
     2101 tcattcctgc cgacctgcac tttgatccgg atggtatcga tctgccatcc gtcttttgac
     2161 agatgttcct gcagtttcgg caactgctgt ttgagtttcg cggcaagcgc cgcattgggt
     2221 acactcagca gcagttgctc gccttcgaca ttcacagcat cgcatgattt gaacatagcc
     2281 ggcagaatcg ctgcacaact tttttgtagc gcagtcatgc gggtaatggc aggcagcaag
     2341 cctgccatta cattatccag ctgtaaaaaa tctgtcacgt ccctgggctt gcgtttcaat
     2401 tgcgcagccg aagggccttg gcgataagga gtaaatgtgg agggatactt catctttcgg
     2461 gcctgttcac attaaattga ggaaatgcac ccaaacgagc gtctttggca ggtccgctat
     2521 ctcgagatgc ctgcatgcta aaatccaagg tttgttggct tcattctgcg gttccgtgcg
     2581 ggaaaaatca ccgcgcagag tcattttccg gcgctttttc agaatccaag catgtcatta
     2641 ctgacccaga ttttcggtag tcgcaatcaa cggctgctca aacaatatca aaaaactgtc
     2701 tatcgaatca atgcgctcga ggcagatatc gagagactgc cggatgccga tctgcaagcc
     2761 aagacgccgg aattcaagca gcgcatagcc agcggcgtca ggcttgatga tctccttccc
     2821 gaagcctttg cggtatgccg cgaagccagc aggcgtgtac tcaggatgcg tcatttcgat
     2881 gttcaattga ttggcggcat ggtgttgcat ttcggcaaaa ctgctgaaat gcgcaccggc
     2941 gaaggcaaga ccctgatggc gacgttgccg gcgtatctga atgccctggc gggcaaaggc
     3001 gtacacatcg ttaccgtcaa cgattatctg gctcaacgcg atgccgaatg gatgggcaag
     3061 ctgtatggct ggcttggttt gagcaccggt atcaacctgt cacagatgga gcatgatctg
     3121 aagcagagcg cctatgcggc ggatatcact tacggcacca acaacgagtt cggtttcgac
     3181 tacctgcgcg acaacatggt gttcgatgct ggccaacgcg tgcagcgcgg cctccatttc
     3241 gccattgtcg atgaagtcga ttcgatcctg atcgacgaag cgcgtacccc gctgatcatt
     3301 tccggtcagg acgaaaacca caccgacctg tatcacaaga tcgatgcagt accaccgttg
     3361 ctgatgcagc agatcggtga agaaaccccc gacggtaaag gcaggatcga agttcccggt
     3421 gattacacca aagatgaaaa aagccatcag gttctactga ccgaggccgg ccacgagaag
     3481 gccgaacaga tcctgaccag catgggcctg ctgccggaag gcgcgtcgct gtatgatgca
     3541 tccaacatct cgctggtgca tcacctgtat gccgccttgc gtgcccacac gctgtatcac
     3601 aaggaccagc aatacgtggt gcagaacggc aatatcgtga tcgtcgacga attcaccggg
     3661 cgcctgatga cgggtcggcg ctggtccgat ggtctgcacc aggccgtgga agccaaggaa
     3721 aaagtcaaga tccagaacga gaattcgacc ctggcctcga tcactttcca gaattacttc
     3781 cgcatgtacg gcaaactgtc gggcatgacc ggcaccgccg acaccgaagc ctacgaattc
     3841 caggaaatct accatctgga aaccgtggtg atcccaccga accgtccgag cgcgcgcaag
     3901 gaccgccagg atcaggtgta taaaagcgcg caggaaaagt acgcggcgat gttgaaagac
     3961 atccaggact gtcacgaacg cggtcagccg gtgctggtcg gcaccacctc gatcgaaaac
     4021 tccgaactac tgtcaggcat tctgaagcag gccgggctgc cgcacaacgt actcaatgcc
     4081 aagcaacacg cgcgcgaagc ggaaatcatt gcgcaagccg gccggcccag gatgatcacc
     4141 atcgccacca acatggccgg ccgcggtacc gatatcgtac tgggcggcaa tgtcgagaag
     4201 cagatccaga tcatcgatgc cgccttgcca gatgccgcag cgcaggcgca aaagctgcgc
     4261 gatgaatggc aggctctgca cgaccacgtg gtcggcgctg gcggcctgca tatcatcggt
     4321 accgaacggc acgaatcgcg ccgtgtcgac aatcagttac gcggccgttc cggtcgtcag
     4381 ggcgatccag gatcgtcgcg tttctatctg tcgctggatg acgccttgct gcgcatcttc
     4441 gccggcgatc gtgtgcgcgc catcatggaa cgcctgaaga tgccggaagg cgaaccgatc
     4501 gaggctggta tcgtcacgcg ctcgatcgaa tcggcacagc gcaaggtgga agcacgcaac
     4561 ttcgacatcc gcaagcaatt gctggaatac gacgatgtcg ccaatgacca gcgcaaggtc
     4621 atctaccagc agcgtaacgc cttgctggaa gctagcgaca tgtcggaaat gatcacctcg
     4681 ctgcgtgacg gcgtatttac cgaactgttc cgtacccacg taccggaaga gtcgatcgaa
     4741 gagcaatggg acatcccggc gctggaagct gcgctgggca acgaatggca actcgccgta
     4801 ccgctgagcg cgatgctgga agccgaaccg aacctgaccg acaaggaact gctggagcgc
     4861 gtgctgtcgg ctgccgaggt ggctcacgat gccaaagcca gggttgtcgg caaggaagca
     4921 ttcaacagtt tcgaacgcag cgtcatgctg caaagcatcg acagccactg gcgtgaacac
     4981 ctggccgcgc tggaccatct gcgccagggt attcatctgc gcggctatgc gcaaaagaat
     5041 ccgaagcaag aatacaagcg cgaagctttc gagctgtttg ccggaatgct cgatctgatc
     5101 aggaacgagg tcatcaagat cgtcatgacc gtgcgcatcg aaaatcgcga agccatccag
     5161 gaggccgaag aacacatggc gcaatcgcat gtcggaaacg tgcattacca gcatgctgat
     5221 ttcgacgctg atacagcgcc ggaagaattg ctggcgccaa tgactcatgc cggggccggc
     5281 gggaaagcgg cgcaattacc agtcaacggt gtacccaagg tcggccgtaa tgatccctgc
     5341 ccttgcggca gcggcaaaaa gtacaagcaa tgccatggca
//
LOCUS       DASRDH010000167         3292 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_350583,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000167 DASRDH010000000
VERSION     DASRDH010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 3292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3292
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350583"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            135..1835
                     /locus_tag="VFA14_03800"
     CDS             135..1835
                     /locus_tag="VFA14_03800"
                     /inference="COORDINATES: protein motif:HMM:NF015803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HZS80538.1"
                     /translation="MQRSICGTSAITVLCVSLHVLLFPGTSAADVRSPGAGNPFDNLP
                     RLKPAPAQTVGGVQIQKQDPDAALRHLLALRIVPSRFQILGVKTLDFNTVAARFAGMA
                     HREVSVAELLQAANEVTQMYKDKGYPLSFAFVPAQTFQDNVVVINVVEAYVGSISIEG
                     DSGASAQRLRDIAGQLKNDRPLSKAMFERITGVLRQQLGMLVNAMVKPPTTTDGAAEM
                     VLQVKRKALTLGVALDNAVSASDVRGIFSVTESGLTPFGEQITVSTLAPGGSSHEQFY
                     GLTYMQPIGRQGMLFQLSASDYSSKPENQSLTVLQFYPRYQSETWRVGANLAYPLMLD
                     NHTNLTLTGGVYGVENMERYTRSVQAAIPLVELGSNIRALSLELAWTRLAEKRGTQLF
                     AGIYRGVDRAGANLTNSQIDLDFTRFKTAFMQTFQLPAEFGLAFSGSGQYSDSILPSS
                     EQIGFGFKLFGLAYPAGEIAGDQGWGLALELNRAIPFEAAWLKQVQPYVMTDASRVYA
                     NSQTLAHDRIASVGAGVRFSGQRYYSFDLSLAKPVGQTPLNADGRPLRLNLSYTYQLD
                     "
     gene            1880..2056
                     /locus_tag="VFA14_03805"
     CDS             1880..2056
                     /locus_tag="VFA14_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003068300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HZS80539.1"
                     /translation="MKNQLIQFLQDEEGSTAIEYGLIAGLIAVGIVASLKSVGGGLQG
                     LFAKIVTAMTGATS"
     gene            2137..2319
                     /locus_tag="VFA14_03810"
     CDS             2137..2319
                     /locus_tag="VFA14_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014897201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HZS80540.1"
                     /translation="MKNQLVRFLQDEEGSTAIEYGLIAGLIAVGIVAALISVSGGLQG
                     LFTSIASALALSGSAS"
     gene            2393..2569
                     /locus_tag="VFA14_03815"
     CDS             2393..2569
                     /locus_tag="VFA14_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003068300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HZS80541.1"
                     /translation="MKNQLIRFLQDEEGSTAIEYGLIAGLIAVGIVASLKSVGGGLQG
                     LFAKIMTSLTGAAS"
     gene            2627..3139
                     /locus_tag="VFA14_03820"
     CDS             2627..3139
                     /locus_tag="VFA14_03820"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HZS80542.1"
                     /translation="MTVLLLSLCIGVLYYDLLFRRVPNSLLLFFLGLDLVCLSVTGHG
                     IGGMDWPQSIIGGIIGLFLFVPLYVLRAMGAGDVKFFAVLGGLLGPQYLIPLWLIGSL
                     LAGAHAGAIYLSRQGMIYLSPGLYRVIEKMQGSALYRRMCHERQGRQGIPYAAYLAIA
                     AILTMAYWSR"
BASE COUNT          675 a          847 c          967 g          803 t
ORIGIN      
        1 tgactattgt ctcgaattaa ttagactatt taatagaaac atagacgatt tggcgcacaa
       61 aatttgatag gactagcgcg agtcgttcct tgcatattac gccggaaaac cagttggttt
      121 aaaaggaaat gcacatgcaa aggtctatct gtgggactag tgcgatcacg gtactttgtg
      181 tatcgctgca tgtgttgctg tttcccggta ccagcgcagc ggatgtacgt agtcccggcg
      241 caggcaatcc tttcgacaat ctccccaggc tgaagcctgc tccggcgcaa actgtcggtg
      301 gcgtccagat ccagaagcaa gacccggatg ccgcgctgcg gcatctgctg gcgttgcgca
      361 tcgttccttc gcgtttccag attctcggcg tcaagacgct ggattttaat actgttgccg
      421 cgcggtttgc cggtatggcg catcgggaag tgagcgttgc cgagctgctg caggccgcca
      481 atgaggtgac gcagatgtat aaggacaaag gttatccgct gtcgttcgcc tttgtcccgg
      541 cgcagacttt ccaggataac gttgtggtga tcaatgtcgt tgaagcttat gtcggctcga
      601 ttagcatcga gggtgactcg ggcgcctcgg cgcagcgttt gcgcgatatt gccgggcagc
      661 tcaagaacga tcgtccactg agcaaggcga tgtttgagcg cattaccggc gtattgcgtc
      721 agcagcttgg gatgcttgtc aacgcgatgg tcaaaccgcc gaccacgacc gatggcgcgg
      781 cggaaatggt tctgcaggtc aagcgtaaag cgttgaccct gggagtcgcc ctggacaatg
      841 cagtctcggc ctccgatgtg cgcggcatct tcagcgtgac tgaaagtggc ctgacgccgt
      901 tcggcgaaca gataaccgtc agcacgctcg cgccgggcgg ttcttcgcac gagcagtttt
      961 acggacttac ctacatgcag ccgataggca ggcagggcat gctgttccag ctcagcgcgt
     1021 cggattacag cagcaagccg gaaaaccaga gcctgacggt gctgcagttc tatccgcgct
     1081 atcagagcga aacctggcgt gtcggtgcca atctggctta tccgctgatg ctggacaacc
     1141 atactaatct caccctcaca ggcggcgtct atggagtgga aaacatggaa cgttacacac
     1201 gctccgtgca ggcagcaata ccgcttgtcg aactaggctc caatattcgt gcattgagtc
     1261 tggagctagc ctggaccagg ctagcggaaa aacgcggcac gcaattgttc gccggcatct
     1321 atcgaggcgt ggatagggca ggggccaacc ttaccaacag ccagatcgat ctcgacttca
     1381 cacgcttcaa aaccgcgttc atgcagacct tccagttacc ggcagagttc ggcctcgcct
     1441 tcagcggcag cgggcagtat agcgacagta tcctgccttc atcggagcag atcgggtttg
     1501 gcttcaagct gttcggtctg gcttatccgg caggcgaaat tgcgggagat cagggctggg
     1561 gactggcgct ggaactgaat cgcgcgattc ctttcgaagc tgcctggctc aagcaggttc
     1621 aaccttatgt gatgacggat gcttcccggg tgtatgcaaa ttcacagacg cttgcccatg
     1681 acaggatcgc ctctgtcggg gccggcgtgc ggttttccgg tcaacgttac tactctttcg
     1741 atctgtcgct ggcaaagccg gtcggtcaga cgccgctcaa tgccgacggc aggccgcttc
     1801 gcctgaatct gtcctacacc tatcaactcg actaggcagg atagacagag tttcattcac
     1861 cactaccctg gagatagcca tgaaaaacca gttgattcaa tttttgcaag acgaagaagg
     1921 ctccactgcc atcgagtatg gtttgatcgc cggtctgatt gctgttggca tcgttgcttc
     1981 cctgaagagt gtcggtggcg gactgcaggg gctattcgcc aagatcgtga ctgccatgac
     2041 cggggctacg tcgtaaggat agagcgatat cgggcgcctg cattgctggc acatcgtgtt
     2101 atgcaagctt cacccatcac cactacggag atagccatga aaaaccagtt ggttcgattt
     2161 ttgcaagacg aagaaggctc caccgccatc gagtatggtt tgatcgccgg tctgattgct
     2221 gtcggcatcg ttgctgccct gatcagcgtt tctgggggac tgcaggggct atttaccagt
     2281 atcgcgagtg ctctggctct gagcgggtct gcgtcttgag gagggagcga tattgggcgc
     2341 ctggccagcc aggatatgca gagtttcacc cgtcaccact atggagatag ccatgaaaaa
     2401 ccagttgatt cgatttttgc aagatgaaga aggctccacc gccattgaat atggcttgat
     2461 cgccggtctg attgctgtcg gcatcgtcgc ttccctgaag agtgtcggtg gcggactgca
     2521 ggggctattc gccaagatca tgacttcttt gaccggggct gcgtcgtgag catcgataaa
     2581 tagagcaata tcgagcgccc ttatttggga tgtatggaga gcgtcaatga cagttttgct
     2641 gctgagtctg tgtatcggtg tgctgtatta cgacctgctt ttcaggcgtg tgccgaacag
     2701 cctgctcctg ttctttctgg ggctggatct ggtgtgcctg agcgtgaccg gacacggtat
     2761 cggcggtatg gactggccgc agtccatcat cggcggcatc atcggtttgt tcctgttcgt
     2821 tcccctgtat gtcttgcggg ccatgggggc tggtgatgtc aaatttttcg ctgtgctggg
     2881 aggtctgctg gggccgcagt atctgattcc gctctggctg atcggcagtc tgttggcagg
     2941 tgcgcatgcc ggcgccattt atttgtcgcg acagggaatg atctacctgt cgccgggctt
     3001 atatcgagtc attgagaaaa tgcaaggcag cgcgttatat cgccgcatgt gtcatgaaag
     3061 gcaaggacgt caaggcattc cgtatgcggc ctatctggct atcgccgcta ttttgacgat
     3121 ggcatattgg tctaggtgaa acagaaaacg cgggtatcga ggaggcaatc atgctgatgt
     3181 cgaccagatt actcaggcag aagcgaaccg ctgtccggcg gcagcatggc gttgccgcgc
     3241 tggaattcgt tcttgttttt ccggttttct ttatcgtttt ttacagcatt gt
//
LOCUS       DASRDH010000168         2066 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_91183,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000168 DASRDH010000000
VERSION     DASRDH010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91183"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..93)
                     /locus_tag="VFA14_03825"
     CDS             complement(<1..93)
                     /locus_tag="VFA14_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS80543.1"
                     /translation="MKVFYDKDADLSLIKNKNVTIIGYGSQGHAH"
     gene            complement(186..677)
                     /gene="ilvN"
                     /locus_tag="VFA14_03830"
     CDS             complement(186..677)
                     /gene="ilvN"
                     /locus_tag="VFA14_03830"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004630835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HZS80544.1"
                     /translation="MRHIVSVLLENEAGALSRVVGLFSARGYNIETLTVAPTEDATLS
                     RMTIVTSGTDDVIEQITKHLNRLIEVVKVVDLTEGAHTERELMLIKVRAVGKEREEMK
                     RMVDIFRGHIIDVTEKTYTIELTGNKGKLDAFIDSIDRAAILETVRTGSSGIGRGERI
                     LRI"
     gene            complement(680..>2066)
                     /gene="ilvB"
                     /locus_tag="VFA14_03835"
     CDS             complement(680..>2066)
                     /gene="ilvB"
                     /locus_tag="VFA14_03835"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HZS80545.1"
                     /translation="QDAFQECDTVGITRPCVKHNFLVKDVKDLAATMKKAFYIAATGR
                     PGPVLVDIPKDISVQTCAYDYPAAVEMRSYRPVDKGHSGQIRKALQLLLSAERPIIYT
                     GGGIILANAAPELNRLVDRLNFPCTNTLMGLGGYRATSDRYVGMPGMHGTYEANMAMQ
                     HSDVMIAIGARFDDRVIGNPGHFASSARKIIHIDIDPSSISKRVKVDIPIVGNVKDVL
                     QELLALLDAADTGPNVAALANWWTQINQWRERDCLKFATSEECIKPQSVVRKVWDITK
                     GDAFITSDVGQHQMWAAQYYGFDKPRRWINSGGLGTMGVGLPYAMGVQKAHPDATVAC
                     ITGEASIQMCIQELATCKQYHLTPKIILLNNRFLGMVRQWQQIDYGSRYSESYMDSLP
                     DFTRLAESYGHVGMKIEKPGDVDGALKEAFDMKDRLVFMNFITDPTENVWPMVKAGKG
                     LTEMLLGSEDL"
BASE COUNT          446 a          573 c          565 g          482 t
ORIGIN      
        1 atgcgcgtgg ccttgcgaac cgtaaccgat gatagtcacg ttcttgttct tgatcaggga
       61 caggtctgcg tctttgtcgt aaaaaacttt catgattttt cctggtgact aaattgttga
      121 aataattgtt gaaatgttga atccttgcgg acatccgata gggaactggc gacgatgcac
      181 gggaatcaga ttctgaggat ccgttcaccg cggccgatac ccgaactgcc ggtacgcaca
      241 gtctcaagaa tcgccgcgcg gtcgatcgaa tcgatgaacg cgtccagctt gcccttgtta
      301 cctgtcagtt cgatggtgta ggtcttttcg gtgacatcga tgatatgacc acggaagatg
      361 tccaccatgc gcttcatttc ctcccgttcc ttgcccacgg cgcgcacctt gatcagcatc
      421 agctcgcgct cggtatgcgc gccttcagtc aaatccacga ccttcaccac ctcgatcagg
      481 cgattcaggt gcttggtgat ttgttcaatc acgtcgtccg taccgctggt cacgatagtc
      541 atgcgcgaca aggtagcgtc ttcggtaggt gcaacagtca gggtttcgat gttgtagcca
      601 cgtgcggaga acagccctac gacgcgggac agagcgcctg cttcgttttc cagcaggaca
      661 gaaacaatgt ggcgcatgat tacagatcct ccgaacccag cagcatttca gtcaaacctt
      721 tacctgcctt gaccatcggc cagacgtttt cggttggatc ggtaatgaaa ttcatgaaga
      781 ccagcctgtc tttcatatcg aacgcttcct tcagcgcgcc atccacatcg cccggttttt
      841 cgatcttcat gccgacatgt ccataggact cggccagtct ggtgaaatca ggcaaggaat
      901 ccatgtaaga ctcggagtag cgcgaaccat aatcgatctg ctgccactgg cgcaccatgc
      961 cgaggaaacg gttgttgagc aaaatgatct tgggcgtcag atgatattgc ttgcaggtgg
     1021 ccagttcctg aatgcacatc tggatcgatg cttctccggt aatacaggcg acggtcgcat
     1081 cgggatgcgc tttttgtacg cccatggcat acggcaagcc gacgcccatg gtgcccaggc
     1141 cgccggaatt gatccagcgg cgcggcttgt cgaaaccata atactgcgca gcccacatct
     1201 gatgctgacc gacgtcggat gtaatgaagg cgtcaccctt ggtgatgtcc catactttcc
     1261 ggaccaccga ttgcggcttg atacattctt ccgaggtcgc aaatttcagg cagtcgcgct
     1321 cgcgccattg attgatctgc gtccaccagt tcgccagggc ggcgacgttc gggccggtat
     1381 cggcagcatc gagcagggcc agcaactcct gcaggacatc cttgacattg ccgacgatgg
     1441 gaatatcaac cttgacccgc ttggaaatcg atgacgggtc gatgtcgatg tgaatgatct
     1501 tgcgcgcgct ggaagcaaaa tgcccaggat tgccgatcac gcggtcatcg aaacgcgcgc
     1561 caatggcaat catcacatcg ctgtgctgca tcgccatgtt ggcttcatat gtaccgtgca
     1621 tgccgggcat gccgacgtat ctgtcgctgg tggcgcgata accgcccagg cccatcaatg
     1681 tgttggtgca ggggaagttc aaacgatcga ccagtctgtt cagttccggc gccgcattgg
     1741 cgagaatgat gccgccgcca gtgtagatga tcggacgctc ggccgacagc agtagctgta
     1801 gcgccttgcg aatctggcca gagtgtccct tgtcgaccgg cctgtacgac cgcatctcga
     1861 cggctgccgg ataatcgtaa gcacaggtct gcaccgaaat atccttggga atatcgacca
     1921 gcaccggacc tggacggcca gtcgcggcga tatagaaagc ttttttcatc gtcgcggcaa
     1981 ggtctttcac gtccttgaca aggaagttat gcttgacgca agggcgggtg atgccgacag
     2041 tatcgcattc ctggaaggcg tcctga
//
LOCUS       DASRDH010000169         4720 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1523502,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000169 DASRDH010000000
VERSION     DASRDH010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 4720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4720
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1523502"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..120)
                     /locus_tag="VFA14_03840"
     CDS             complement(<1..120)
                     /locus_tag="VFA14_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZS80546.1"
                     /translation="MARLPRLLVPHQAHHLIQRGHDRQSVFCDGADHAAFLEWL"
     gene            complement(142..1107)
                     /locus_tag="VFA14_03845"
     CDS             complement(142..1107)
                     /locus_tag="VFA14_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003071071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HZS80547.1"
                     /translation="MSSVESFASPLMWGGFIVFVLGMLAFDMLALGGRHAHKVTVREA
                     LGWSIAWVMLTLLFSGLLWWYLDQSIGRAFAQQKSLEFLSGYLIEKSLSVDNIFVFLV
                     IFGYFGVPPEMQRRVLLYGVLGAIIMRAFMILFGAWLIAQFSWIMYLFGAFLVVTGIK
                     MLIFADKESDLDNNPLLKFLRRHLRISNEYDGEKFFTLRNGVRYFTPLMLVLILIEMT
                     DLVFAVDSIPAIFAITTDPFIVFTSNMFAIMGLRALYFLLADVADRFHYLKYGLALVL
                     MFVGGKMLLADWFHLPVMISLLVVAVILTASVLVSLALSTRKPSS"
     gene            1493..1756
                     /locus_tag="VFA14_03850"
     CDS             1493..1756
                     /locus_tag="VFA14_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosyl-(dinitrogen reductase) hydrolase"
                     /protein_id="HZS80548.1"
                     /translation="MRNKLDHKNPSVTEKEIAECFDNKCGINLIDNREDNQTDPATLW
                     FIAETNRGRLLKVVYIFKDEKCHIKTAYDPNEVEIDIYEKYGK"
     gene            1746..2168
                     /locus_tag="VFA14_03855"
     CDS             1746..2168
                     /locus_tag="VFA14_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HZS80549.1"
                     /translation="MVNKVVPPLVMPQQEKQMKTPEKIKGTVDNWEGGLLGRDKNYVK
                     SAPKELERQVDEAQGMQAISIRLDRDLIEKFKQIAKIHNMGYQPLMREALKRFADAEI
                     KIILAMVANASEKTAGNDGKQTVELELDDLQQCRVAYN"
     gene            complement(2490..3569)
                     /locus_tag="VFA14_03860"
     CDS             complement(2490..3569)
                     /locus_tag="VFA14_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013236451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZS80550.1"
                     /translation="MPELSVNDLHLDYGSGAGASPILKGVSMQLQRGEVVTLLGPSGS
                     GKTTLLRAVAGLESPKAGAIDIAERRVFDGARSFDMPAEERNLGLVFQSYALWPHKTV
                     FDNVAYGLKLRRMSNSEISERVKTVLSQLGLGHLGRRFPHQLSGGQQQRVAIARALVY
                     NPPVILLDEPLSNLDAKLREEARAFLRELIVRLGLSALMVTHDQNEAMAISDRILLLN
                     NGRIEQQGTPQSMYQTPDTLFTAEFMGSNNRLKGKVLQRDGRRVTLQVAGGTVVASAR
                     GANTGNDVTGIIRLEQVRIEDHQADNAIRLPLATCMYLGDRWECLFKAGNADSTTVGL
                     RAYAPHRLEQGEYWLTLPEQALWAF"
     gene            complement(3620..4720)
                     /locus_tag="VFA14_03865"
                     /pseudo
     CDS             complement(3620..4720)
                     /locus_tag="VFA14_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013204997.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
BASE COUNT         1259 a         1329 c         1229 g          903 t
ORIGIN      
        1 gagccactca aggaaagctg catggtcggc gccatcacaa aacacggatt gccggtcgtg
       61 gccgcgctgt atcaggtgat gcgcttgatg cgggacaagc agacggggca ggcgtgccat
      121 aggaaaagat gttctaaaag atcaactgga cggcttcctt gtcgacagcg ccagactgac
      181 cagcaccgaa gctgtcagaa tgactgccac caccagcagc gatatcatga cgggaagatg
      241 gaaccagtca gccagcagca ttttgccacc gacaaacatc aacaccaatg ccagaccgta
      301 tttcaggtaa tggaaacgat ccgccacatc cgccagcagg aaatacagcg cccgcaaacc
      361 catgatcgca aacatattgg acgtaaacac gatgaaagga tccgtggtaa ttgcaaaaat
      421 cgccgggata ctgtccaccg caaacaccag atccgtcatt tcgatcaata tcagcaccag
      481 catcaacggc gtgaagtaac gcacgccatt gcgcagggtg aagaatttct caccgtcata
      541 ttcgttactg atacgcagat gcctgcgcaa aaatttcagc agtgggttgt tgtcgagatc
      601 gctctcctta tcggcgaaaa tcagcatttt gataccggtg acgaccagga acgcgccaaa
      661 cagatacatg atccagctaa attgcgcaat cagccaggcg ccaaacagaa tcatgaacgc
      721 acgcatgatg atcgcaccaa gtacaccata cagcagtacg cgccgctgca tttccggcgg
      781 cacgccgaag tagccgaaga tcaccaggaa cacaaagatg ttatcgaccg acagcgattt
      841 ttcgatcagg tagcccgaca gaaactccag gctcttctgc tgtgcaaatg cgcgcccgat
      901 actctgatcc agataccacc acaataaacc gctgaacaac aacgtcagca tcacccacgc
      961 aatcgaccag cccagcgctt ccctgaccgt caccttatgc gcatgacgtc cgcccagcgc
     1021 cagcatgtcg aatgccagca tgcccagcac aaatacaatg aaaccacccc acatcaacgg
     1081 actggcaaaa ctttccacac tgctcactgc atttctcctt gcttatttca tcgaggttta
     1141 gtcgaaatca aaaatctcac ccgggaaact cttttctctc ggcttatatc gctggcgtat
     1201 tgtaaaacga aggatctaca gactcagctc gggcggcata cgggggatag gaacgcaagc
     1261 acaggttcta cggcaggcaa caccatggcg tatgaatcat caatggaata acgcttcccc
     1321 ttgcctgcca atgtatatac acgtataatc ggcgtcatgt taagctgcaa cttgcagtta
     1381 caccagaaaa atcataaaaa tggtgactat tcattgaaat taggatcata gaacctatat
     1441 tacgtatata caccaaggaa tgtgaatgaa aggactcgtt atctcatcgg cagtgcgaaa
     1501 taaattggat cacaagaatc catcagtgac ggaaaaagag attgcggagt gtttcgataa
     1561 taagtgtggc ataaatctca tcgataaccg cgaagataat caaactgatc ctgcaactct
     1621 atggttcata gcagaaacga atcgaggccg gttgctaaag gtcgtatata tttttaaaga
     1681 cgaaaaatgt cacatcaaga ctgcgtatga cccaaatgaa gttgagatag atatctacga
     1741 aaaatatggt aaataaagta gtgccaccac tggtaatgcc ccaacaggag aaacaaatga
     1801 agaccccgga aaaaattaaa ggaaccgtag ataactggga aggcggtcta ctcgggagag
     1861 acaaaaacta cgtgaaatca gccccgaagg agctggaacg tcaagtcgat gaagcacaag
     1921 gtatgcaagc gatttccatt cgcctagatc gtgacctcat tgagaagttt aaacagattg
     1981 caaaaatcca caatatggga tatcagccct tgatgcgcga agcgctgaag cgttttgcag
     2041 atgcagaaat taagattatt ttagcaatgg ttgccaatgc aagcgagaag accgccggca
     2101 acgatggcaa gcaaactgtt gagcttgagc tggacgattt gcagcaatgc cgtgtagcgt
     2161 ataactaaat gccgctacga aaaaaattcc attagaccaa gtatgaatgg aacgaattta
     2221 ggagcaaatc atgaaagcag caaagatact cgtcggactg atgatcgcga ttagcgcaag
     2281 cgcggtgctg cggcaatgaa gtcatagaag cacaacgaac cggagagcag cgaacatgtt
     2341 ccagaccctc aaagacctct tcagcatgac cgacaaagcc gctccgcctg caccagcagc
     2401 gacaggactg ctgttaccaa taatttcttt tgcaccttac tgttcccttt ggtaagaatc
     2461 gtttttctgg ctgtgttgcc aacacattct tagaaagccc acagggcctg ttcaggcaag
     2521 gtcagccagt actccccctg ttccagcctg tgcggcgcat aagcgcgcaa gccgacagtc
     2581 gtgctgtccg cgttcccggc tttgaacagg cactcccacc ggtcccccag atacatgcag
     2641 gtggcaagcg ggaggcggat ggcgttgtcg gcttgatgat cctcgatacg gacctgttcg
     2701 agacgaatga tgccggtgac gtcattgccg gtattcgcac cgcgcgcact ggcgacgacg
     2761 gtgccgcccg ccacctgcaa ggtgacgcgc cggccatcgc gctgcaacac cttgcccttg
     2821 agacgattgt tgctgcccat gaattcggcg gtgaacagcg tatccggtgt ctggtacatg
     2881 ctttgcggcg tgccttgctg ctcgatcctg ccgttgttga gcagcaggat gcgatccgaa
     2941 atcgccatcg cctcattctg gtcgtgcgtc accatcaagg ctgacagccc cagacgcacg
     3001 atcagttcgc gcaggaacgc acgcgcttct tcgcgcagtt tggcgtcgag attggacaag
     3061 ggttcatcga gcaaaatgac cggcgggttg tacaccagcg cgcgcgcgat cgccacgcgc
     3121 tgttgctggc cacccgacaa ctggtgcgga aagcgcctgc ccagatggcc caggccgagc
     3181 tgcgacagca cggttttgac ccgttccgag atctcgctgt tgctcatcct gcgcagcttg
     3241 aggccatagg cgacattgtc gaacacggtc ttgtgcggcc acagcgcata ggactggaac
     3301 accaggccga gattgcgctc ttcagccggc atgtcgaaac tgcgcgcgcc atcgaacacg
     3361 cgccgctcgg cgatatcgat ggcgccagcc ttgggcgatt ccaggccggc cactgcgcgc
     3421 agcaaggtag tcttgccgct gccggacggt cccagcaggg tgactacttc accgcgctgc
     3481 agttgcatgg agacgccctt gagaatcgga ctggcgccgg cgccactgcc gtaatcgagg
     3541 tgtaaatcgt tgacgctgag ttctggcatg gagattcctt tgaaaacgcg cagcggcaag
     3601 gctgccgcat gagatgggat cagtcgtgca gcttgatgcc gaaacgcagg gcgatgccga
     3661 ggccgatcgc caccagtgtg atgttgatga aagacagcgc cgccaccagt tccaccgcgc
     3721 cggcggccca catcgagacg atcatcgagc cgatcacttc agtgcctggt gacagcagat
     3781 acacgccggt cgaatattcg cgtgaaaatc aggaacacca tcagccatgc gcccagcaga
     3841 ccgtaccgta ccagcggcag cgtcacatcg cgcgtgacct ggccgcgttg cgcgccaacc
     3901 gcgcgcaccg cttcttccag ttccggtccg acttgcagca gggccgtcgt gatcaggcgc
     3961 aggccatatg ccatccatac taccgaatac gccagccaga ccgagaagat ggtcgagcgc
     4021 agtgaacgca gttgtggaat gaagttgtcg accagccagc gcgccacatc gttgtcgaaa
     4081 ccgcgaagga agtcgtttag cagtgatggc acaaacagga acacccacag gaaagacaga
     4141 ccggccagca aacctggcac cgcgcgcggc accagtacgc tgtagtcgag gaagcgggtg
     4201 acgccgtcat tctggcgatg catcgccaga gcgatcgcgc tatagcacat catggcaagc
     4261 gcgccgccga tgataccgat caggacggta ttgatgatgc cgcgtaccag cgatggctgg
     4321 ctccagatat cacggaaatt ttgcagcgta aacacttcca gcaatgcgac gccatcgccc
     4381 cagtgcgaca cgaaagtacg caacgcaatg cccgacagcg gcacgatcac cgtcaacagc
     4441 agccatgcgg caatcagcgt gaaagcgatc cagcgccatt tacccagcgg cagcgccttc
     4501 tggtgcgcgc ccttgccctt gatcgacaca tatttgttgg ctgacttcag cagccagcgc
     4561 tgcaacatca ccagcggcag gctcactgct accagacaca ctgctaccgc cgccatcaga
     4621 tgatacgaag gcgtaccgag cttgttggtc aatttgtaga gataggtggg caacaccaga
     4681 tggccttcgg ggtcgccaag caccagcacc aggccgaaca
//
LOCUS       DASRDH010000170         3385 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1537587,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000170 DASRDH010000000
VERSION     DASRDH010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 3385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3385
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1537587"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1054
                     /gene="mraY"
                     /locus_tag="VFA14_03870"
     CDS             <1..1054
                     /gene="mraY"
                     /locus_tag="VFA14_03870"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008445432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HZS80551.1"
                     /translation="AGPTVIRMLTLLKVGQAVRTDGPQTHLVKSGTPTMGGVLILIGI
                     GMSTLLWADLSNRFVWIVLIVTLGFGAIGWVDDYRKVVYKDPNGMRSREKYFWQSLIG
                     LVAAFYLAFSVSEVNNIKVLDLFLAWVQSGFSLDLPPKADLIVPFFKTASYPLGVWGF
                     IALTYFVIVGTSNAVNLTDGLDGLAIMPTVLVGGALGIFAYLTGSANYARYLFIPHIP
                     GAGELLIYCGAMAGAGLAFLWYNAYPAQVFMGDVGALALGGALGTIAVIVRQEIVLFI
                     MGGIFVVETLSVMIQVTYFKFTRKRYGVGKRVLLMAPLHHHYEQKGWKETQVVVRFWI
                     ITMMLVLFGLSTLKLR"
     gene            1077..2693
                     /gene="murD"
                     /locus_tag="VFA14_03875"
     CDS             1077..2693
                     /gene="murD"
                     /locus_tag="VFA14_03875"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HZS80552.1"
                     /translation="MNYADKHALVLGLGESGLAMARWLVYCGARVRVADTRGEQAVAE
                     RLATLRAASTHAVFIGGQAFVAGLLDGIDFVAVSPGLAPERDLALLSAAAAEKNIPLW
                     GEMELFAQALAALREERAYAPKVLAITGTNGKTTVTSLTGRLCRRAGLTTRVAGNIGP
                     AALDVLREALIEEKAHAEAVASASVTVVPLQREAAPQEVERVYPGRLPQAWVLELSSF
                     QLHTTYSLQADAAAVLNVTQDHLDWHGSMDAYIADKARIFGDKTVRVLNRGDAVVMKM
                     ATPDATVSGFGLDEPVKAGDFGLVDENGMLWLSTAVLLEDEENPEGRRRRKDRQDASE
                     PAFLLRRLMPADALRIRGLHNALNAMAALALCRAAGLPLADLLHGLRDYAGESHRTEL
                     VATIGDVAYYNDSKGTNVGATVAALNGFGHGGKPNRLLLIAGGDGKGQDFSPLAEPLS
                     KYGRAVFLIGRDAGAIRAAVADTGITLIDCTTLEAAVEQAAGMARGGDAVLLSPACAS
                     LDMFRNYVHRAEVFIDAVREIALSHGEVTG"
     gene            2804..>3385
                     /locus_tag="VFA14_03880"
     CDS             2804..>3385
                     /locus_tag="VFA14_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HZS80553.1"
                     /translation="MMEYDQPLVWVVVLLMLFGMVMVYSASIALHDSPKYANYKTSHF
                     LVRQAMFIGVSLLLSLMVFRIRIETWQTVAPYLFVGTLILLTLVLIPGVGKGVNGAKR
                     WLSLGIFNVQPSELMKLFIVLYAADYTVRKQEVMHKLTRGFMPMMLAVGVVGLLLLLE
                     PDLGAFGVIVCIAMGILFLGGINGIWFGGIGAML"
BASE COUNT          599 a          910 c         1112 g          764 t
ORIGIN      
        1 cgccggtccg acggtgatcc gcatgctgac tttgctcaag gttggccagg cggtgcgcac
       61 ggatggtccg caaacgcatc tggtcaaatc cggcacgccg accatgggcg gcgtgctgat
      121 tttaatcggt atcggcatgt cgactttgct gtgggcggat ctgagcaatc gtttcgtctg
      181 gatcgtgctg atcgtcacac tcggtttcgg cgccatcggc tgggtcgacg attaccgcaa
      241 ggtggtctat aaggatccga acggcatgcg ttcgcgggaa aaatatttct ggcaatcgct
      301 gatcggtctg gtagcggcat tttatctggc gttctcggtg tccgaggtga ataacataaa
      361 ggtgctcgac ctgttcctgg cctgggtaca gtcaggcttc agtctcgatt tgccacccaa
      421 ggccgacctg atcgtgccgt tcttcaagac cgccagctat ccgctgggcg tatggggttt
      481 catcgcattg acttacttcg tcattgtcgg caccagcaac gcggtcaacc tgaccgatgg
      541 cctggatggc ctggcgatca tgccgactgt gctggtcggc ggcgcgctcg gtatttttgc
      601 ttacctgacc ggtagcgcca actatgccag gtatctgttc attccgcata tcccgggagc
      661 cggcgagttg ttgatctact gcggcgccat ggccggcgca ggtctggctt tcctctggta
      721 caacgcttat ccggcacagg tgttcatggg tgacgtcggc gcgctggcgc tgggcggcgc
      781 actcggcacc atcgccgtga tcgtgcgtca ggaaatcgtg ctcttcatca tgggcggcat
      841 ttttgtggtg gaaaccttgt cggtgatgat tcaggtgacg tatttcaaat tcaccagaaa
      901 acgctatggc gttggcaagc gcgtgctgtt gatggcgccg ctgcatcatc attatgagca
      961 aaaaggctgg aaagaaacac aagtcgtggt gcgcttctgg atcattacga tgatgctggt
     1021 gttgtttggt ttatcgacgc tgaagctgcg ttgatcgaga actcgggtag ggatggatga
     1081 attacgcgga taaacacgct ctggtactgg gactgggtga atccggtctg gcgatggcgc
     1141 ggtggctggt ctactgcggc gcgcgcgtgc gtgtggccga cacgcgtggc gagcaggccg
     1201 tggcagaacg tctggcgacc ttgcgcgcgg catcgacaca tgccgtgttc atcggcggtc
     1261 aagcctttgt ggccggattg ctggatggca tcgatttcgt cgccgtcagt cctggtctgg
     1321 cgccggaacg cgatctggcg ctactgtcgg cggctgcggc agagaaaaat atccccttgt
     1381 ggggcgagat ggaattgttt gcgcaagcgc tggcagcatt gcgcgaagag cgcgcctacg
     1441 cgcccaaggt attggcgatc accggcacga acggcaagac tacggtcacc agcctgaccg
     1501 gcaggctatg ccgccgcgcc gggctgacaa ccagggttgc cggcaatatt ggcccggccg
     1561 cgctggacgt gctgcgcgaa gccttgatcg aagagaaagc gcatgccgag gcggtagcat
     1621 cggcgtctgt gacggtagta ccgcttcagc gcgaagcggc gccgcaggaa gtggagcgcg
     1681 tctaccccgg caggctgccg caagcctggg tgctggaatt atcgagcttc cagttgcata
     1741 cgacctacag cctgcaggcc gatgccgcgg ctgtgctgaa tgttacgcaa gaccatctgg
     1801 actggcatgg cagcatggac gcctacatcg ccgacaaggc aagaattttc ggtgacaaga
     1861 ccgtgcgcgt actcaaccgt ggcgatgccg tggtaatgaa gatggcgacg cctgacgcga
     1921 ccgtgtcggg tttcggtttg gatgaaccag tcaaggctgg cgatttcggt ttggtcgatg
     1981 aaaacggtat gctgtggctg agcactgccg tgttgcttga agacgaagaa aaccccgaag
     2041 gccgccgccg caggaaagat cggcaggacg cgtccgaacc agccttcctg ctcaggcgct
     2101 tgatgcctgc cgacgcgctc aggattcgtg gcttgcataa tgccctcaac gcaatggcgg
     2161 cgctggcatt atgccgcgcg gccggtttgc cgttggcgga cctgttgcat ggtttgcgcg
     2221 actatgccgg cgagtcgcac cgcaccgaac tggtagctac cattggcgat gtcgcctatt
     2281 acaacgacag caagggcacc aatgtcggcg ctaccgttgc cgccctgaat ggcttcggtc
     2341 acggtggcaa gccgaatcgt ctgctgctga tcgccggcgg cgatggcaag ggtcaggatt
     2401 tctcgccgct ggccgagcca ttgtcgaaat acggtcgcgc cgtcttcctg attggccgcg
     2461 acgctggggc gatacgtgcg gcggtggccg ataccggcat cacgctgatc gattgtacga
     2521 ccctggaggc cgccgtcgaa caggccgccg ggatggcgcg aggcggtgat gccgtgttgc
     2581 tgtcgccggc ctgcgccagc ctggatatgt tccgcaacta tgttcatcgc gccgaagtct
     2641 tcatcgatgc tgtgcgtgaa attgcgttgt cacatggcga ggtgacggga tgagaccgag
     2701 cctgcaattg attactcgct ggtttggtgg tggggccgct acgcgctcaa gtgcgcgcgg
     2761 cggcagcctg aaaggcgtag ccggcatcga gcagcgctcg cgcatgatgg aatatgacca
     2821 gccgctggtg tgggtagtcg tgttgttgat gctgttcggc atggtcatgg tgtattcggc
     2881 atcgattgcc ttgcatgact ctcccaagta tgcgaattac aagacttcgc attttctggt
     2941 gcgtcaggcg atgttcatcg gcgtgtcgtt gctgctcagc ctgatggtgt ttcgcatcag
     3001 gatcgagacc tggcaaacgg tggcgccgta tctgttcgtg ggaaccctga tcctgttgac
     3061 cctggtgctg attcccggcg tcggcaaggg tgtcaacggt gccaagcgct ggctgtcgct
     3121 cgggatattc aatgtgcagc cgtcggaatt gatgaaactg ttcatcgtgc tgtatgccgc
     3181 cgattacacc gtgcgcaagc aggaagtgat gcacaagctg accagaggtt tcatgccgat
     3241 gatgctggcg gttggcgtgg tcggtctgct gctgttgctg gagccagacc tcggcgcctt
     3301 tggcgtgatc gtgtgcatcg ccatggggat tttattcctg ggcggcatca acggtatctg
     3361 gtttggcggt atcggcgcga tgctg
//
LOCUS       DASRDH010000171         9740 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_620770,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000171 DASRDH010000000
VERSION     DASRDH010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 9740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9740
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_620770"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1139)
                     /gene="gshA"
                     /locus_tag="VFA14_03885"
     CDS             complement(<1..1139)
                     /gene="gshA"
                     /locus_tag="VFA14_03885"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HZS80554.1"
                     /translation="MVPHLVTALNGPLLDLEKKILDATPAIERWFRMEWQEHTPPFYC
                     SVDLRNAGFKLAPVDTNLFPGGFNNLSPEMLPLAVQAAMAAIEKYCPDARNLLLIPEN
                     QYTRNTFYLQNLARLIQIFRQTGLNVRLGALSGNIEQPGNINLPDGSTMMLEPLERSR
                     NGRRLGLKNFDPCTILLNNDLSGGIPAVLENLNEQNVLPPLHAGWAVRRKSNHFAAYD
                     EVVKKFSKLVDVDPWMLDPLFAKCEEIDFQTGAGIDCLADNVSSLLSKIRRKYKEYGI
                     KEQPFVIVKANAGTYGMGIMTVRDANEVKDLNHRQRDRMALGKEGLKVSDVILQEGVH
                     SFERINEAVAEPVVYMIDRYVIGGFYRVHADRDVNENLNAPGAHFV"
     gene            1375..2454
                     /gene="hemE"
                     /locus_tag="VFA14_03890"
     CDS             1375..2454
                     /gene="hemE"
                     /locus_tag="VFA14_03890"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HZS80555.1"
                     /translation="MPSFAPLKNDTFLRALLRQPTAYTPLWLMRQAGRYLPEYRATRA
                     RAGSFLKLAKTPDYATEVTLQPLERYPLDAAILFSDILTIPDAMGLGLHFTDGEGPKF
                     EHPLRDEMAVLALQAPPLDKLRYVFDTVTQIRTELDGRVPLIGFSGSPWTLACYMVEG
                     GGSDDFRTVKTMLYDRPDLMHHLLKINAVAVAAYLNAQIDAGAQAVMIFDTWGGVLAD
                     GIYQGFSLRYMADVLRDLKREKDGVRIPVIIFTKGGGLWLEQIAAIGADAVGLDWTVN
                     LADARRRVGNRVALQGNLDPHALLAGPAQIEAEVRKLLHTSGKPEAGSGYVFNLGHGI
                     SQFTPPEAVTILVQAVHRISRELRQ"
     gene            3222..5257
                     /locus_tag="VFA14_03895"
                     /pseudo
     CDS             3222..5257
                     /locus_tag="VFA14_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017449807.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
     gene            complement(5399..6061)
                     /locus_tag="VFA14_03900"
     CDS             complement(5399..6061)
                     /locus_tag="VFA14_03900"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HZS80556.1"
                     /translation="MIGSLPQFIRFAEKSSDVWGIRDTKSRYVYANRAGRELVNLPPG
                     FEIEGKTDSEIPHPIAEFEKELQAFDRLVHVTKEAKRTLEIHPWGKEQVVSPYYGETL
                     PLFDENGEVTGTIFYGHLALNLRFNRDKIDAGTFIFGAPDDTLSKKEWDFVYFVMRGY
                     SIKEMARLSEVSPGTIASRQNSVFNKLGIDTLAQLKQMAIAKGWDNYIPTDLLRNKFI
                     KL"
     gene            complement(6128..6793)
                     /locus_tag="VFA14_03905"
     CDS             complement(6128..6793)
                     /locus_tag="VFA14_03905"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HZS80557.1"
                     /translation="MIMPLKQLIHFAENAAEPWCAKDTASRFLYANQAYLELHGLPHD
                     FEITGLRVGELPVPAAALAKEFEASDRLVETTKDAKRSLEIYPRGKDQVLSAYYFNKY
                     PLFDKSGEFIGTAVHGSPALDLRFNRDGVDVQTVTFGVPNDTLSKEEWSFAYLVTRGH
                     SIEEIARLSASSPEAISRRQDAVFNKLGIDTLEQLEQMVFEEGWDRCIPSDLLHGRYI
                     ELS"
     gene            7065..7149
                     /locus_tag="VFA14_03910"
     tRNA            7065..7149
                     /locus_tag="VFA14_03910"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7099..7101,aa:Leu,seq:gag)
     gene            7250..7609
                     /locus_tag="VFA14_03915"
     CDS             7250..7609
                     /locus_tag="VFA14_03915"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008450355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HZS80558.1"
                     /translation="MSLENYFPVLLFILVGIGVGIAPQLLGRLLGPHRPDPQKTSPYE
                     CGFEAFEDARMKFDVRYYLIAILFILFDLETAFFFPWGVSMRDLGWSGYVTMMIFIAE
                     FVVGFWYIWKKGALDWE"
     gene            7623..8225
                     /locus_tag="VFA14_03920"
     CDS             7623..8225
                     /locus_tag="VFA14_03920"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020748138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HZS80559.1"
                     /translation="MATKLETLEAALRNALGEYLQNLTVVLGEITIAVRPADYLAVMR
                     VLRDHAEVRFEELIDLCGVDYSAYGDSAWEGPRFAVVSHLLSISHNWRVRVRVFAPDD
                     AWPLVASVTGIWSSANWYEREAFDLFGILFDGHDDLRRILTDYGFIGHPFRKDFPVSG
                     YLEMRYDAGQKRVVYQPVTIEPREITPRIIREENYGIKHG"
     gene            8218..9471
                     /locus_tag="VFA14_03925"
     CDS             8218..9471
                     /locus_tag="VFA14_03925"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HZS80560.1"
                     /translation="MAEIKNYTLNFGPQHPAAHGVLRLVLELDGEVIQRADPHIGLLH
                     RATEKLAEQKTYLQSVPYMDRLDYVSMMCNEHAYVMAIEKLLGIEVPLRAQYIRVMFD
                     EITRLLNHLMWIGSHSLDVGAMGVLLYAFREREDLMDCYEAVSGARMHAAYYRPGGVY
                     RDLPDTMPQYQASIMRSGKAIERLNENRQGSLLDFIEDFTRRFPGCVDEYETLLTDNR
                     IWKQRLVGIGVVSPERALDMGFTGPMLRGSGVEWDLRKKQPYAVYDLLDFDIPVGRNG
                     DSYDRYLVRVEEMRQSNRIIRQCIEWLRKHPGPVIIDNHKIAPPARVDMKSGMEDLIH
                     HFKLFTEGFHVPPGETYAAVEHPKGEFGIYLVSDGANKPYRIKIRAPGFAHLSSLDEM
                     ARGHMLADAVAIIGTQDVVFGEIDR"
     gene            9548..>9740
                     /locus_tag="VFA14_03930"
     CDS             9548..>9740
                     /locus_tag="VFA14_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012401393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HZS80561.1"
                     /translation="MLSQDALKKIDRELAKYPENQRQSAVMSALRIAQDEHGWLPAGL
                     QQEIADYIGMPAVAVQEVAT"
BASE COUNT         2096 a         2625 c         2706 g         2313 t
ORIGIN      
        1 acgaaatgcg caccgggcgc gttcagattc tcgttgacgt cgcggtctgc atggacacga
       61 tagaaaccgc cgatcacata acggtcgatc atgtacacca ccggttcggc caccgcttca
      121 ttgatgcgct cgaaagaatg caccccctcc tggaggatga catcgctgac tttcagacct
      181 tccttgccga gcgccatccg gtcgcgttgc ctgtgattga gatccttgac ttcgttggcg
      241 tccctgacgg tcatgatgcc catgccgtaa gtgccggcgt tggctttcac gatgacaaaa
      301 ggctgctcct tgatgccgta ttctttatac ttcctgcgga ttttcgacag caggctgctg
      361 acattgtcgg ccaggcagtc gataccggca ccggtctgga aatcgatctc ttcgcacttg
      421 gcaaacaaag gatcgagcat ccacggatcg acatcgacca gcttggaaaa tttcttgacc
      481 acttcgtcgt aggcagcgaa gtgattgctc ttgcggcgca ccgcccagcc tgcatgcagc
      541 ggcggcagca cgttctgctc gttgaggttt tccagcactg cgggaatgcc gcccgacaga
      601 tcgttattca gcagaatggt gcagggatcg aaattcttca ggccaagacg acgaccgttc
      661 ctggaacgct ccagcggctc cagcatcatg gtgctgccat cgggtagatt gatgtttcca
      721 ggctgctcga tgtttcctga caaggcgcca agacgtacat tgagaccggt ctggcggaag
      781 atttgtatca gtcgcgcgag gttctgtaga tagaaggtgt tacgtgtgta ctggttttca
      841 ggaatcagca gcaagttcct ggcatccgga cagtatttct cgatcgcggc cattgcggcc
      901 tgtaccgcca gcggcagcat ttccggcgac agattgttga agccgcccgg aaacagatta
      961 gtgtcaaccg gcgccagctt aaaaccggca ttacgcaaat cgaccgaaca gtaaaatggt
     1021 ggcgtgtgtt cttgccactc catgcggaac cagcgttcga tcgcgggtgt ggcatccaga
     1081 attttctttt ccaggtccag aagtggaccg ttgagggcgg tgacgagatg cggaaccata
     1141 aaaaaccttc tcagtacaaa acagcggcag gagagtcgag aaccaagccg gcaaaagctg
     1201 accattccag cgggcttctt gctatgacat cgttcaccat tcggtaattt ctggtggaga
     1261 aatttcagac atggcggcgt tgtcgctttt ttaaagagca atcaggatag aaaatctgat
     1321 gcgcggtttg gatgagataa tgggcaatta ccaacttgtc caacacgatt gcccatgcct
     1381 tcgttcgcgc cactgaaaaa cgataccttc ttacgcgcct tgctgcgcca gccgactgca
     1441 tacacgccac tgtggctcat gcgtcaggct ggccgttatc tgccggaata tcgcgctacg
     1501 cgcgcgcgcg ccggctcgtt cctgaaactg gccaaaactc ccgattatgc taccgaagtc
     1561 acgctccagc cactggaacg ttatccgctg gatgccgcga tcctgttttc cgacattctc
     1621 accatcccgg acgcgatggg cctgggtctg catttcacgg atggtgaagg accgaaattc
     1681 gagcatccgc tgcgcgatga aatggccgta ctggccttgc aggcgccgcc actggacaaa
     1741 ctgcgctacg tcttcgacac cgtcacgcag atccgcactg agctggatgg tcgtgtacca
     1801 ctcattggtt tctcgggaag cccgtggaca cttgcgtgct acatggtcga aggcggtggt
     1861 tcggacgact tccgcaccgt caagaccatg ctctacgatc gtccggatct gatgcatcac
     1921 ctcctcaaga tcaatgcagt ggcggtcgcg gcatacttga atgcccagat cgatgccggc
     1981 gcgcaagccg tgatgatttt cgatacctgg ggcggtgtgc tggccgatgg catatatcag
     2041 gggttttcgc tgcgttacat ggccgacgtt ctgcgcgacc tcaaacgcga gaaggacggc
     2101 gtccggatcc cggtcatcat attcaccaaa ggcggtggtt tgtggctgga acagattgcc
     2161 gccatcggcg ctgatgccgt cggcctcgac tggacggtca atctggccga tgcgcgtcgc
     2221 cgtgtcggca acagggtcgc tctgcaaggc aatctcgatc ctcatgcctt gcttgccggc
     2281 cctgcgcaga tcgaagcgga agtgcgcaaa ctgctgcaca cttccggcaa gcccgaagct
     2341 ggtagcggtt atgtcttcaa tctcggccac ggcatctcgc aattcacacc acccgaggcg
     2401 gttaccatac tggtgcaagc cgtccacagg ataagtcgcg aactgcgcca ataaaaacgt
     2461 gggtgtatgc cgtatgcaaa cagataggcg tcagcttgta aaagaccgga gacaatcatc
     2521 atttgcatga cttatgcaca aagcctcaac ttgtttcatc aatatcgtca tttgttctat
     2581 tgagcaagat attttccata aactgttgat tttaaatgat tttatttcct acgcaggttt
     2641 gatgcagacc atgaaaacca gcatgacacg ggagacaatg gctgtcgtcg ggtgacttgt
     2701 caacaaaatt atccacagga ttgatagaga gttgaacaag caactgatac agccggcatt
     2761 gatgtttttg cgctgatcgt ttttacttga tatgcatggg cgcagccgat ttctcggcaa
     2821 ggctgttccg agatggctta gagccgctaa cgtaatgtcg ctggctgcgt tacgccttct
     2881 tgccgtacct tttgtactgt cttcgtcgac acgccttgcc agcggcgttg tgttaacggc
     2941 tcttatttgg acttatgcac aaaaccgttt tatgcgcagc agtaataaaa aagtcaaaga
     3001 aaatttctgc attttgcggg atattttata gatcattgat tttaaatgaa aaaattttca
     3061 tcttctgagg cgggcaaggc atggaaaccg gtatccagct tggagcaaaa gctgtcaagc
     3121 ccattttgtc cacaaaatta tccacagagt ctgtggatag cgaaaaagat agtttatgaa
     3181 acagccagtt aggtcatttc ttcaggaatt acattaagtt cgtgaaatac cgcatactcc
     3241 agatcgcgct ggatactccc cttgacagga tttttgacta tcgctggcgc cgtagcgcgg
     3301 acgaagtggt accgcaggtt ggacaattgg cgatcgtgcc tttcggtcac cgcgaggtcg
     3361 ccggtctcat cgtcggcgtt gccgaagata cggacgtgcc gcagaacaag ctcaaggatg
     3421 tcgtcgccgt gcgcagtcaa ttgctcccgc tgtcggtgca atggctggct ttatgcagtt
     3481 tttccgccgc ttactaccag cgtccgttgg gcgaagtggc gttgcccagc ctgcccagaa
     3541 atctgcgtgc gctgaccacg acagcgcttg atcgcgctct gaagaaactg ggcaaaccgg
     3601 gcgtaacaca gaatactcat ccagtcagca caccgccatt gaaagcggag cagcaggatg
     3661 ctatcgatgt catcgccggt gcgcagggtt ttgcgccctt gctgttgtac ggcgtcaccg
     3721 gcagtggcaa gaccgaggtc tatctgcagg cagccgcgcg tattctggcg cgcagcgaaa
     3781 cggccggcga acaggcgcag atcctgatac tggtaccgga aatcaacctc acgccccagc
     3841 tcgaatcgac tattcgcgcg cgttttccgg gcgtgtatgt ggtgagtctg catagcggtc
     3901 tggccgaggg agaacgcacg ctcaactggc tggcggcgca tcatggtcag gcgcgtatcg
     3961 tgctcggcac gcgcctggcg atcctgtcgt cgctgccgca tctcaaactc atcgtcatcg
     4021 atgaagagca tgatccatcc tataagcagc aggaaggctt gcgctattcg gcgcgcgacc
     4081 tcgccgtttg gcgcgcgcat caactgggca ttccagtcgt attaggctcg gctacgccat
     4141 cgctggaagc ctggcagcat gtccaggccg gacgctaccg caagctgcca ttgcgcacgc
     4201 gcgcggtacg cgatgccgtc ctgcccagag tgaagataat cgatatggag cgcgaccgcc
     4261 ccaacgatgg cttgacatcg acgctggtgg cggcactcag gctgcgcttg gaacgcggcg
     4321 agcagtcact gttgtttctc aaccggcgcg gttatgcgcc ggtgctgcac tgcgacgcct
     4381 gcggctggat cagcaactgc aagcgttgca cctcatttat ggtattgcac aagccagagc
     4441 ggcatttgcg ctgtcaccat tgcagcctgg aactacgcgt tcccgaagcc tgcccgacct
     4501 gcggcagcgt cgatttgcag ccactgggac gcggcacgca acgcatcgaa gagggcttgc
     4561 aggcgatctt tccagaagcg cgcattctgc gcatcgatgc cgattcgacg cgcctcaagg
     4621 gcgctgctcg gacggctttc gacagtgtgc atcgcggtga tgtcgatatg ctgatcggta
     4681 cccagatggt cgccaaaggg catgacttca agcgcttgac gctggtcggc atcctgaatc
     4741 cggacacggc gctgttttcg catgactacc gcgccagcga acggctgttc gcgcaactga
     4801 tgcaggtcgc cggtcgtgct ggccgggccg ggccgaaaga aggcggcttg cctagcgagg
     4861 tgctgatcca gagccgctat ccgcatcatc tttatatggc gcggtcaccg cgcatgacta
     4921 tgacggcttc gccgcccggt tgctggagga gcgccggcag gccgcgttgc cgccgttcat
     4981 ctaccaggcg ctgctgcgtg ctgaagcagg cggactcgcc gtcgccctgg catttttgca
     5041 ggatgccgca gcctgtctgg aacatcccga cattatcatc aacgacccga ttccgatgac
     5101 catgacgcgc gtagctaatg tcgagcgcgc ccagttgctg atcgagtgcg cctcgcggcc
     5161 cgcattgcag gcttttctgc gcgactggct ggtagtgttg cgcgccatga aaacacgcgc
     5221 caagtggtcg ctggaagtcg acccggtcga tatttgatgc gtttgacccc attcggcatt
     5281 catggcatga aacacggttg tctcgccgga tcggagttac ctgatctgta tgatcaggaa
     5341 cggggaacca tgacttcgtt tcatcatgaa aaccggatga tgctggaaga acttgtcatt
     5401 atagcttgat aaatttgttg cgtagcagat cggtcgggat atagttgtcc cagcccttcg
     5461 ctatcgccat ctgtttgagc tgggcaagtg tatcgatgcc tagtttgttg aagacggaat
     5521 tctgcctgct ggcaatcgta cccggtgaga cttctgacaa gcgcgccatt tccttgatcg
     5581 aataaccgcg cattacgaaa tagacgaaat cccactcttt tttggacagg gtatcgtccg
     5641 gtgcgccgaa aataaaagtc cccgcatcta tcttgtctct gttgaaccgc aggttcaacg
     5701 ccagatgacc gtagaagatc gtgccagtta cttcaccatt ttcgtcaaat aatgggagtg
     5761 tctcgccata ataaggtgac accacctgct cttttcccca tggatgaatt tccagagtcc
     5821 gcttcgcctc tttagtgacg tgaaccaagc ggtcaaacgc ttgaagttct ttctcgaatt
     5881 cagctatcgg atgaggtatt tcgctatccg ttttgccttc gatttcaaag ccgggcggca
     5941 agttcaccag ttctcgcccg gcccggttgg catacacata acgggacttc gtatctctga
     6001 ttccccatac gtctgaggac ttctctgcaa aacggatgaa ctgcggcaat gaccctatca
     6061 tgattttctc ctttgaaaat tgaggtttgt cgagacgtta cacagaaacg caaccgggca
     6121 gattttgtca cgacagctcg atgtacctgc cgtgcagcag atcgctcggg atgcagcggt
     6181 cccagccctc ctcaaatacc atctgttcga gctgctcgag cgtatcgatg cctaatttgt
     6241 tgaaaacagc atcctgcctg cgggaaatcg cctccggcga gcttgctgac aagcgcgcta
     6301 tttcctcgat tgaatgacca cgtgtcacaa ggtaggcgaa actccattct tcttttgaca
     6361 gggtatcgtt cggcacgcca aaagtaacag tctgcacatc cacgccgtcc ctgttgaatc
     6421 gcaggtccag cgccggagag ccgtgaacgg ccgtgccaat gaactcacca cttttgtcga
     6481 acaatggata tttgttgaaa taataagctg acagcacctg gtcttttccc cgcgggtaaa
     6541 tttccagaga ccgcttcgcg tctttggtgg tttcgaccag acgatcagat gcttcaaact
     6601 ctttcgcaag tgcagccgct ggaacaggca gttcgccgac ccgtaaacct gtgatttcaa
     6661 aatcgtgcgg caagccatgg agttccagat aagcctggtt cgcatacagg aaacgggaag
     6721 ccgtatcttt ggcacaccat ggttctgcag cattctccgc aaaatggata agctgcttca
     6781 atggcattat catgattttc tcctttgaag atgttggtgc ttcgcgcagg cagtcatacc
     6841 tggcggcggg aatagggcat gatcagcata gttttgagct gtgatccaca aatcgataca
     6901 gtagttccgg atgtcagcaa cgcttgtaaa tcgatctctg tatcctgccg gaacaaggga
     6961 tggccttgat catggatgca ttgtcgggtt tttcgcgcgc aagctgggca tgctttttat
     7021 gtgttgaaca ggagcatcga aatgcattac aatcaccttc tttggccgac gtggtgaaac
     7081 tggtagacac gctatcttga gggggtagtg gcgaaagctg tacgagttcg agtctcgtcg
     7141 tcggcaccat gacaaatcgg cgagggaaga tgagctggtt tttttcgagg aaaaatctgt
     7201 ttgatatcaa gcgtcgtttc gctgactgtc gacaaatctg tgcctaatcg tgagcctaga
     7261 gaattatttc cccgttctat tgtttatcct ggtcggcatc ggtgtcggca tcgctcctca
     7321 attactcggt cgtctgcttg gtcctcacag gcccgatccg caaaagacct ctccttatga
     7381 atgcggcttc gaggcatttg aagatgcgcg catgaagttc gatgtgcgtt attacctcat
     7441 cgccattctc ttcattctgt tcgatctcga aacggcattt ttcttcccgt ggggtgtttc
     7501 catgcgcgac ctcggttggt ccggctacgt cacgatgatg atattcatcg ctgaattcgt
     7561 tgtcgggttt tggtacatct ggaaaaaagg cgccctggac tgggagtgag aggaagggca
     7621 ggatggcgac aaaactggaa actctcgaag ctgccttgcg caacgcgctt ggggagtatc
     7681 ttcaaaatct gacagtggtt ttgggcgaga tcacgatcgc ggtcaggccg gccgattact
     7741 tggcggtgat gcgtgtgctg cgcgatcatg ccgaagtccg tttcgaagag ttgatcgacc
     7801 tgtgcggtgt ggattattca gcttatggcg acagtgcgtg ggaaggtccg cgctttgccg
     7861 tggtgtccca tctgctgtcg atcagtcata actggcgcgt ccgcgtccgt gttttcgcac
     7921 ctgatgatgc ctggccgctg gtggcctcag tgactggtat ctggtcttca gccaactggt
     7981 atgaaaggga agcctttgat cttttcggta tcctgttcga tggacacgac gatttacgtc
     8041 gcattctgac agattatggt ttcatcggtc acccgttccg caaagatttc ccggtgtcgg
     8101 gttatctgga aatgcgctac gatgccgggc aaaaacgcgt ggtttatcag cccgtgacga
     8161 tcgagccgcg cgagattacg ccgcgtatca tccgtgaaga aaattacggg atcaaacatg
     8221 gctgaaatca agaactatac gttgaatttc ggtccccagc acccggctgc gcatggtgtg
     8281 ctgcgtctgg tgctggagct ggacggtgag gtcattcaac gcgctgaccc gcatatcggc
     8341 ctgctgcatc gcgcgacaga aaaacttgcc gaacaaaaga cttacctgca atcggtgcct
     8401 tatatggacc gtctcgacta cgtgtcgatg atgtgtaacg agcatgccta cgtgatggcg
     8461 atcgaaaagc tgctcggtat cgaagtgccg ctgcgcgcgc agtacatccg tgtcatgttc
     8521 gacgaaatca cgcgtctgct gaaccacttg atgtggatcg gttcacacag tctggatgtc
     8581 ggcgccatgg gcgtgctgct gtacgccttc cgcgagcgtg aggatttgat ggattgctac
     8641 gaagccgtgt ccggcgcgcg catgcatgct gcctactatc gtccgggcgg cgtttatcgc
     8701 gacctgcctg acacgatgcc gcagtaccag gcatcgatca tgcgcagcgg caaggctatc
     8761 gaacggctca atgaaaatcg tcaaggctcg ctgctggatt tcatcgaaga ttttacccgg
     8821 cgtttccccg gctgtgtcga cgaatacgaa accctgctga cggataaccg tatctggaag
     8881 cagcgtttgg tcggcatcgg cgtggtgtcg ccggagcgcg cgctggacat gggtttcact
     8941 ggccccatgc tgcgcggttc cggcgtcgaa tgggatctgc gcaaaaagca gccttacgca
     9001 gtctacgacc tgctcgattt cgacattccg gtcggcagga acggcgacag ctacgaccgt
     9061 tatctggtgc gcgtggaaga aatgcgtcag tccaaccgta tcatcaggca atgcatcgaa
     9121 tggctgcgca aacatccagg cccggtgatc atcgacaacc acaagatcgc tccgcctgcg
     9181 cgcgtggata tgaaatccgg tatggaagac ctgatccacc acttcaagct gttcacggaa
     9241 ggttttcatg ttcctccggg tgagacctat gctgcggtcg agcatccgaa gggggagttt
     9301 ggcatctatc tggtgtcgga cggcgccaac aagccgtacc gcatcaagat tcgcgcgcct
     9361 ggttttgcgc atttgtcgag tctcgatgaa atggccagag gtcacatgct tgccgatgcc
     9421 gtagccatca ttggcacgca ggacgtggta tttggcgaaa tcgacaggta gagcgtgttc
     9481 caggcggtca ctggatgttt ttgaaaacct gtcagcctgt ggggaagcac ggatgaccaa
     9541 cgatatgctg ttaagtcaag acgctctaaa aaaaatcgat cgcgaactcg cgaaatatcc
     9601 tgagaatcaa cgccagtccg cggtcatgtc tgcgctgcgc atcgcccagg atgaacacgg
     9661 ctggctgcct gccgggctgc agcaggaaat cgccgactac atcggcatgc ctgcggtggc
     9721 ggtgcaggaa gttgccacgt
//
LOCUS       DASRDH010000172         3518 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_707045,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000172 DASRDH010000000
VERSION     DASRDH010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 3518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3518
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707045"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1393
                     /locus_tag="VFA14_03935"
     CDS             <1..1393
                     /locus_tag="VFA14_03935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS80562.1"
                     /translation="SLGCLKRTLITHVEEIPVENRNEHIHVLTGNLLSTLLDQGASVE
                     SLFQLYRQVISATRQGKDYVFDKKFSLLSKLITQAPKKFHVVFAVDNVSNAGSFPNAI
                     GSVNFLSQPPAANNPKPPVIRYLTAYPKRLFAKVEVETKDYRTAGTEAYAAINNILDL
                     VRFEYERERLHLPDEFVITSSEQNQNYRIFPIPKVVPNPIASVDNAGLQEFVQSVNEL
                     MANSGFQEDGSSRVQSAFRQYRIGSDTHIFENKLINWWTAVEYLAKGSNAQGGIGQAV
                     ENALTPVLCLGYINKLLLSFRNALVDLKVQLTDQTTRQLIQLKDLTPKEIYQLFKTPA
                     QHPDIFASIKDPFLKHKLKNFLDSISTPKDTEIMLKAHEQRLRWHIQRLYRARCNIVH
                     SAERIVNAALLCANLEFYLKMVLTELLRLIHAIPQISGPKEFFDRQTYAYDKLMADLR
                     TNKDDRLLLHLQK"
     gene            complement(1521..2723)
                     /gene="pcaF"
                     /locus_tag="VFA14_03940"
     CDS             complement(1521..2723)
                     /gene="pcaF"
                     /locus_tag="VFA14_03940"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006415854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0019619 - 3,4-dihydroxybenzoate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipyl-CoA thiolase"
                     /protein_id="HZS80563.1"
                     /translation="MTEALICDAIRTPIGRYGGVLKEVRADDLGAIPLRALMMRNPQV
                     DWNAIDDVIFGCANQAGEDNRNVARMSALLAGLPQSVPGSTINRLCGSGMDAVGTAAR
                     AIRSGEAQLMLAGGVESMTRAPFVMGKADSAFSRSTGMYDTTIGWRFPNPQMRVLHGT
                     DTMPETAENVADDFRISRPDQDRFAVASQAKAAAAQANGTLAHEITPVVIPQKKGDPL
                     TVINDEHPRATSIEALARLKGVVRADGTVTAGNASGINDGACALLLANAAAVNRYGLT
                     PRARIIGMATAGVAPRIMGIGPAPATRKLLLQCSLSIEQMDVIELNEAFAAQSLAVLR
                     ELGVADDDPRVNPNGGAIALGHPLGMSGARLVTTAMYQLQRSGGRYALCTMCIGVGQG
                     IAMLIERV"
     gene            complement(2739..3389)
                     /locus_tag="VFA14_03945"
     CDS             complement(2739..3389)
                     /locus_tag="VFA14_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit B"
                     /protein_id="HZS80564.1"
                     /translation="MQRFSRDQIAARVAQDIPEGAYVNLGIGLPTKVANYLPADKEIL
                     LHSENGLLGMGPAPAPGAEDEDLINAGKQPVTLLTGGAYFHHADSFAMMRGGHLDICV
                     LGAFQVSATGDLANWHTGAADAIPAVGGAMDLATGARQVFVMMEHQTRNGESKIVSHC
                     SYPLTGIACVNRIYTDLAVLDVTKQGLQVREIVADLSFEELQKMTGAPLLPAVAQP"
     gene            complement(3389..>3518)
                     /locus_tag="VFA14_03950"
     CDS             complement(3389..>3518)
                     /locus_tag="VFA14_03950"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013396544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HZS80565.1"
                     /translation="AARCTIAQVNEVVELGQLDPESIITPGIFVQRIVQPAAQEEK"
BASE COUNT          803 a         1011 c          953 g          751 t
ORIGIN      
        1 ttcattgggc tgcctgaaac gcactttgat tacccatgtt gaggaaattc cggtggagaa
       61 cagaaacgaa catatccacg tattgaccgg gaacctgcta agcacccttc ttgatcaagg
      121 cgcgagtgtc gaatcgctat tccaactgta ccggcaagtc ataagcgcta cacgacaggg
      181 aaaagactac gttttcgaca aaaaattcag cttactatcg aaactgatta cccaagctcc
      241 aaaaaaattt cacgtcgtgt tcgcagtcga taacgtctca aacgctggct ccttccccaa
      301 cgccatcggc agcgtgaatt tcctgtcgca accaccggca gccaacaatc caaagcctcc
      361 cgttattcga tacctcaccg cgtatccaaa gcgattattc gcaaaagtcg aagtggaaac
      421 gaaagattat cgaaccgccg gaaccgaagc ctatgccgcg atcaacaaca tcctggatct
      481 ggttcgattc gaatatgaac gcgaacggct gcatttgcct gacgagtttg tcattacatc
      541 cagtgagcaa aaccaaaatt atcgaatttt cccgattccc aaagttgttc ccaaccccat
      601 cgcgtcggta gataatgcag gactgcaaga atttgtacag agtgtcaatg agctgatggc
      661 taattctggt tttcaggaag acggaagcag ccgggtgcaa tcggcgtttc gccaatatcg
      721 catcggttcc gatacgcaca tctttgaaaa caaactcatc aactggtgga ccgcagttga
      781 atatctggca aaaggcagca atgcccaagg cggaattggc caagctgtcg aaaatgcgct
      841 gacgcccgtg ctctgtcttg gctacataaa caaattattg ctttcatttc gcaatgcact
      901 cgttgacctg aaagtccaat taaccgacca gacgacgagg cagcttatac aattgaaaga
      961 cctaacaccc aaggaaattt accaactgtt caaaacccca gcgcaacatc cggacatctt
     1021 cgcatcaatc aaggatccct tcctcaaaca taagctgaaa aatttccttg actcgatttc
     1081 cacccccaaa gataccgaga ttatgctcaa ggcccatgag cagcgcctgc gttggcacat
     1141 acaacgcctg tatcgcgcac gatgcaacat cgttcacagt gcagaacgga tcgttaacgc
     1201 agcactgttg tgcgcgaatc tggagttcta tctgaaaatg gtgcttaccg aattgctgcg
     1261 tttaattcat gcaattcccc aaatctctgg cccaaaggag tttttcgatc gtcaaacata
     1321 cgcgtatgac aagcttatgg cggatcttcg caccaataaa gatgaccgat tattgttgca
     1381 ccttcagaaa taacggcata catgcagtga aaaagtgggg ctgtcatagc acaaatcgac
     1441 gagggcctac gtttttttgc tggcgacgag atcctggcat tgattcagat ttgcctgcaa
     1501 tgtcgtatgc ttaccaatct tcaaacacgc tcgatgagca tcgcgatacc ctgcccaacg
     1561 cctatgcaca tggtacacag ggcataacga ccgccgctgc gctgcaactg atacattgcc
     1621 gtcgtcacca ggcgcgcacc actcatcccg agcggatggc cgagcgcaat cgcgccgccg
     1681 ttcggattga cgcgcgggtc gtcgtcggcc acgccgagct cccgcaatac cgccagactc
     1741 tgggcagcga aagcttcgtt gagttcgatg acatccatct gttcgatact cagactgcat
     1801 tgcagcagca gcttcctggt ggcgggcgcc gggccgatac ccatgatgcg cggcgctacg
     1861 ccggctgtcg ccatgccgat gatgcgcgcg cgcggcgtca ggccatatcg gttgaccgca
     1921 gcggcgttgg ccagcaacaa ggcacaggcg ccgtcattga tgccggaggc attacccgcg
     1981 gtcacggtgc catcggcgcg cactacgccc ttaagtcggg ccagggcctc gatactggtt
     2041 gcgcgcggat gttcgtcgtt gatgacggtg agcgggtcgc cttttttttg cgggatcacg
     2101 accggcgtga tttcatgtgc cagcgtgcca ttggcctggg ccgcggcggc cttggcctgg
     2161 ctggcgacgg cgaacctgtc ctgatcggga cgactgatcc tgaaatcgtc ggcaacgttt
     2221 tcagcggttt ccggcatggt atccgtaccg tggagaaccc tcatctgcgg attcgggaaa
     2281 cgccagccga tggtcgtgtc atacatgccg gtgctgcgcg aaaacgcgct gtccgccttg
     2341 cccatgacga acggcgcgcg cgtcatcgac tcgacgcccc cggccagcat cagttgcgct
     2401 tcacccgacc tgatagcgcg tgcggcagta ccgacggcat ccatgccgga accacacagc
     2461 cggttgatgg tggaacccgg caccgattgc ggcaggccgg ccagcagcgc cgacatgcgc
     2521 gcgacgttac ggttgtcttc accagcctgg ttggcgcagc cgaaaatgac atcatcgatg
     2581 gcattccagt cgacttgcgg attgcgcatc atcaaggcgc gtagcggaat ggcgccgagg
     2641 tcatcggcgc gtacttcttt cagtacgccg ccgtaacggc cgatgggagt acggatggca
     2701 tcgcagatca aggcttcagt catggcgtcg tcttctggtc atggttgcgc cacagcaggc
     2761 agcaacggtg cgccggtcat tttttgcaat tcttcaaagg acaggtccgc aacgatttca
     2821 cgcacctgca ggccctgttt cgtcacatcg agcacggcga gatcggtgta gatacggttg
     2881 acacaggcga tgccggtcaa tggatacgaa caatgcgaga cgatcttgct ttcgccgttc
     2941 ctggtctgat gctccatcat gacgaacact tgcctagcgc cggtggccag atccatggcg
     3001 ccgccgacgg caggaatggc gtcggcggcg ccggtgtgcc agttggcgag gtcgcctgtc
     3061 gccgatacct ggaaagcgcc tagcacacag atatcgaggt ggccgccgcg catcatggcg
     3121 aacgaatcgg catggtggaa gtaggcgccg ccagtgagca aagtcacggg ctgcttgccg
     3181 gcgttgatca ggtcttcgtc ttctgcgccg ggcgcgggcg ccggccccat gccgagcaga
     3241 ccgttttcgc tgtgcaggag aatttccttg tcggctggca ggtaattggc aaccttggtc
     3301 ggcaagccga tgccgagatt gacgtaagcg ccttcaggaa tatcctgcgc tacgcgcgcg
     3361 gcgatctgat cgcgggaaaa gcgctgcatt atttttcctc ctgcgcagcc ggttgcacga
     3421 tacgctgcac gaatatgcct ggcgtaatga tggactcggg atcgagctga ccgagttcga
     3481 cgacttcgtt cacctgggcg atggtacacc tggccgcc
//
LOCUS       DASRDH010000173         4154 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_622451,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000173 DASRDH010000000
VERSION     DASRDH010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 4154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4154
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_622451"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="VFA14_03955"
     CDS             <1..546
                     /locus_tag="VFA14_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HZS80566.1"
                     /translation="NLVVRRQGKGTFVATHHEAHAQFRFLRLALNVEEAQHVENRVIE
                     VKRMRAPAELARQLDMKPGDSVIFIKRVKSFNNVPVILEDIWLPGLTFKGLTAERLME
                     YKGPLYGLFEAEFGTRMIRAVERIRAVGAAPESAEFLEVPPQTPLLSVDRISYTYGDK
                     PVEIRHGLYLTSNHHYRSELS"
     gene            616..1026
                     /gene="sdhC"
                     /locus_tag="VFA14_03960"
     CDS             616..1026
                     /gene="sdhC"
                     /locus_tag="VFA14_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HZS80567.1"
                     /translation="MSDAVKPPRPQFRNIHITQIVGYRLPLAGVVSILHRISGLLMFL
                     LLPCVLYLLDRSLLSEISFEHFKSFTSHWFVKLVILALSWSYLHHFCAGIRHLIMDKH
                     IGLDKDNARESAASVLIVSLALTTLLALKLFGAF"
     gene            1029..1394
                     /gene="sdhD"
                     /locus_tag="VFA14_03965"
     CDS             1029..1394
                     /gene="sdhD"
                     /locus_tag="VFA14_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HZS80568.1"
                     /translation="MANHHIGPKRLVVGAHYGMRDWLAQRATAIIMALYTIILLLCFL
                     TSSNFSYEGWAGMFSHQWFKMATFATFMALFYHAWVGVRDIWMDYVKPVALRLALQVA
                     TMVWLIACAGWATQILWRV"
     gene            1397..3175
                     /gene="sdhA"
                     /locus_tag="VFA14_03970"
     CDS             1397..3175
                     /gene="sdhA"
                     /locus_tag="VFA14_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014193768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HZS80569.1"
                     /translation="MAAIRSTIPVRRFDAVIVGAGGSGMRASLQLAEAGLNVAVLSKV
                     FPTRSHTVAAQGGIGASLGNMSEDNWYWHMFDTVKGSDYLGDQDAIEFMCREAPKAVY
                     ELEHFGMPFDRNSDGTIYQRPFGGHTANFGEKPVQRACAAADRTGHALLHTLYQRNVR
                     ARTHFFVEWMAIDLIHDQEGNILGVVALEMETGEIMTLQAKTTLLATGGAGRIWAAST
                     NAFINTGDGMGMAARAGLPLQDMEFWQFHPTGVAGAGVLITEGVRGEGGILINSNGER
                     FMERYAPTLKDLAPRDFVSRSMDQEIKEGRGVGQHKDHVLLDLRHIGAETIQKRLPSI
                     LEIAHKFANVDATREPIPVVPTIHYQMGGIPTNVHGQVVVPKHGSYKEVVQGMYAIGE
                     CACVSVHGANRLGTNSLLDLVVFGRAAGNHIVNSRLQDSSHRDLPADAADRALERLMH
                     LENSTGSERVQDVANAIRTTMQHYCGVFRTDELLQEGYRKIMELDERRKYISFKDKSK
                     VFNTARVEALELDNLIETAKATITSAAARKESRGAHAHRDYEKRDDENWLTHTLWFSE
                     GNRLDYKPVNMQPLTVETFTPKARTF"
     gene            3187..3897
                     /locus_tag="VFA14_03975"
     CDS             3187..3897
                     /locus_tag="VFA14_03975"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020701958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HZS80570.1"
                     /translation="MTRTLKFKIYRYDPDKDERPYMQDLSVELKPSDKMLLDALLRIK
                     SDVDDSLALRRSCREGVCGSDAMNINGKNGLACTTNLNELTEPIVLKPLPGLPVIRDL
                     IVDMTNFFKQYDSIKPYLINDSIPPEKERLQSPEQREELDGVYECILCACCSTSCPSF
                     WWNPDKFVGPAGLLQAYRFIADSRDEATGKRLDDLEDPYRLFRCRSIMNCVDVCPKGL
                     NPNQAISKIKELMVRRAI"
     gene            3941..>4154
                     /locus_tag="VFA14_03980"
     CDS             3941..>4154
                     /locus_tag="VFA14_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HZS80571.1"
                     /translation="MSRYHQDDPAKRARLRWRARRGLLENDLILTRYLDTHEATMTDD
                     EVDAFSRLMDLTDNDLMDLLLMRKEPD"
BASE COUNT          963 a         1184 c         1107 g          900 t
ORIGIN      
        1 aatctggtgg tgcgccgaca gggtaaaggg acattcgtgg cgacccatca tgaagcacat
       61 gcacagtttc gctttttgcg cctggcgcta aatgtagaag aagcgcagca tgtggaaaac
      121 agagtcattg aagtcaagcg tatgcgtgcg cctgccgagt tggcgcgcca gctcgacatg
      181 aaaccgggtg actccgtcat tttcatcaag cgagtaaaat cctttaacaa cgttccggtc
      241 atactggaag atatctggtt acccggcctg actttcaaag ggctgaccgc agagcgcttg
      301 atggaatata aaggaccgct gtacggcctg tttgaagccg aattcgggac gcgcatgatt
      361 cgcgccgtag agcggattcg tgccgtaggg gcggctccgg aatctgcgga atttctggag
      421 gtgccgcccc aaacgccctt gttgagtgtg gatcgcatct cttatacata tggcgacaaa
      481 ccggtagaaa tacggcatgg cctgtatttg acatcgaacc accactatcg aagcgaactg
      541 agctaagcgc cgaaaaagac ttcgtaaaat cgatagaaat tcgatattgt tctaaaaatc
      601 tgaggagatc ctatcatgtc tgatgccgtt aaaccaccac ggccacaatt tcgtaacata
      661 cacatcaccc agattgtagg ttaccgcttg ccactggcag gcgtagtttc tattttgcac
      721 cgtattagcg gcctgttgat gttcctgctg ctaccgtgcg ttctgtatct actggatcgc
      781 agtctgctct ccgaaatttc tttcgaacac ttcaaaagct tcacatcgca ctggttcgtg
      841 aagcttgtca ttctggcgct gtcctggagc tatctgcatc atttctgcgc cggtattcgt
      901 catctcatca tggacaaaca tattggtctc gacaaagata acgcgcgcga gtccgctgcc
      961 agcgttctga tcgtcagtct ggcgctcact accctgctcg cattgaaact gttcggagca
     1021 ttctagaaat ggcaaatcat catatcggac ccaaacgcct cgtcgtgggt gcgcactacg
     1081 gcatgcgcga ctggctggcg caacgcgcta ccgccatcat catggcgctg tacaccatca
     1141 tcctgctgct ctgttttctg acttccagca atttttccta tgaaggctgg gcaggaatgt
     1201 tttcacatca gtggttcaaa atggcgacct tcgccacctt catggccttg ttctaccacg
     1261 cctgggttgg cgtgcgtgat atctggatgg attatgtcaa accagtcgct ctgcgtctgg
     1321 ccctgcaagt tgccacgatg gtgtggctga ttgcttgcgc cggatgggcg acgcagattt
     1381 tgtggagagt gtaatcgtgg ctgctatcag atccacaatt cctgtacgcc gcttcgatgc
     1441 ggtcatcgtt ggtgccggcg gctccggcat gcgcgcttcg ctgcaactgg ctgaagcagg
     1501 tctgaacgtc gcagttttgt ccaaggtttt cccgacccgc tcgcacacgg tcgctgccca
     1561 aggcggcatc ggcgcctcgc taggcaacat gtcggaagac aactggtact ggcatatgtt
     1621 cgataccgtc aagggttcgg actatctggg cgaccaggat gctatcgaat tcatgtgccg
     1681 cgaagcaccg aaggcggtct acgaactgga acacttcggc atgcctttcg atcgtaattc
     1741 tgacggtacg atttatcagc gtccattcgg cggccatacc gccaacttcg gtgaaaagcc
     1801 ggtacaacgc gcttgcgccg ctgctgaccg tactggtcat gcgctgctgc acacactgta
     1861 tcaacgcaat gtacgcgcca gaacccactt ttttgtcgag tggatggcaa tcgacctcat
     1921 ccacgaccag gaaggcaaca tcctcggcgt cgtcgccctg gaaatggaaa ccggcgaaat
     1981 catgacactc caggccaaga cgaccctgct cgccaccggt ggcgcaggcc gtatctgggc
     2041 tgcatcgacc aacgccttca tcaataccgg cgatggcatg ggtatggcgg cgcgtgccgg
     2101 tctgcccttg caagacatgg aattctggca attccacccg accggcgtcg ctggcgcagg
     2161 tgtgctgatc accgaaggcg tgcgcggtga gggtggcatc ctgatcaaca gcaacggcga
     2221 acgtttcatg gagcgctatg cgccgacact gaaggatctg gcgccgcgtg acttcgtatc
     2281 gcgctcgatg gaccaggaaa tcaaggaagg ccgcggcgtc ggtcagcaca aggatcatgt
     2341 gctgctggat ctgcgccata ttggcgcgga aaccatacaa aagcgtctgc cgtcgattct
     2401 ggaaatcgcg cacaaatttg ccaacgtcga tgcgaccaga gaacctatcc ctgtcgtgcc
     2461 gaccatccat taccaaatgg gcggtattcc aaccaatgtc catggccaag tcgtcgtgcc
     2521 gaagcacggt tcgtacaagg aagtggtgca aggcatgtat gctatcggtg aatgcgcttg
     2581 cgtgtcggtc cacggcgcca accgcttggg caccaactcg ctgctcgatc tggtggtgtt
     2641 cggccgcgct gccggcaatc atatcgtcaa tagcagactg caagacagca gccaccggga
     2701 tctgcctgcc gatgcggcag accgcgcgct ggaacgtctg atgcatctgg aaaacagcac
     2761 cggttccgag cgcgtgcagg atgtcgccaa cgcgattcgc accaccatgc aacactattg
     2821 cggcgtgttc cgtaccgacg agctgctgca ggaaggctac cggaaaatca tggaactgga
     2881 cgagcgccgc aaatacatat cgttcaagga caagtccaag gtcttcaaca ccgcccgcgt
     2941 tgaagcgctg gaactggaca acctgattga aacagccaag gcgaccatca cttccgccgc
     3001 cgcccgcaaa gagtcacgtg gcgcgcacgc tcaccgcgac tatgaaaagc gcgacgacga
     3061 aaactggctg acgcacacgc tgtggttttc cgaagggaat cgtctcgact acaagccagt
     3121 caacatgcaa ccgttgaccg tcgaaacgtt tacacccaaa gcacgtactt tctaaaaggt
     3181 cagaacatga cacgtaccct gaaattcaaa atctatcgct acgatccaga caaagatgaa
     3241 aggccataca tgcaggatct aagcgtcgag ctgaaaccga gcgacaagat gctgctggac
     3301 gccctgctgc gcatcaagtc cgacgtcgat gattcactgg cactgcgccg ctcctgccgc
     3361 gaaggcgtgt gcggctccga tgcgatgaac atcaacggca agaacggcct ggcctgtact
     3421 accaatctga acgagctgac agaacccatc gttctgaagc ccttgccggg cttgccggtg
     3481 attcgcgatc tcatcgtcga tatgaccaat tttttcaagc agtacgattc gatcaagcct
     3541 tatctgatca acgacagtat tccaccggaa aaagagcgtc tgcagtcgcc cgaacagcgt
     3601 gaagagctcg atggcgtata cgagtgcatc ctgtgcgcct gctgctcgac atcgtgcccg
     3661 tcattctggt ggaatccgga caagttcgtc ggtcctgccg gcctgttgca ggcttaccgc
     3721 ttcatcgccg acagtcgcga cgaagccact ggcaaacgcc tggacgatct ggaagatcca
     3781 tatcgcctat ttcgttgccg ctccatcatg aactgcgtcg atgtctgccc caaaggtctg
     3841 aaccccaacc aggccatcag caagatcaag gaattgatgg tacgccgcgc aatataagaa
     3901 gcgtgttaca aaacttacct gaaagaggta aattcaagcc atgtctcgat accatcaaga
     3961 cgatcctgcc aaacgggcgc gcttgcgctg gcgcgcccgt cgcggcttgc tggaaaacga
     4021 tctgatcctg acgcgctatc tcgacacaca cgaagccacc atgactgacg acgaagtcga
     4081 tgcgttttcc cgtctgatgg atttgacaga caacgatctg atggacctgc tcctcatgcg
     4141 caaagaaccc gacg
//
LOCUS       DASRDH010000174         2143 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_291843,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000174 DASRDH010000000
VERSION     DASRDH010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291843"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(182..568)
                     /locus_tag="VFA14_03985"
     CDS             complement(182..568)
                     /locus_tag="VFA14_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HZS80572.1"
                     /translation="MAHEQEQPVNPGLIAGLLGIARNFLGLMINRIELAALEFSGIGR
                     NLVRFLVLFTLAAISLWFALAFWTALVVFLAWESWGWKIFVAFGIFFTVLTALIILYA
                     RPILRQGCLSLPATMTELRKDRDALL"
     gene            complement(586..891)
                     /locus_tag="VFA14_03990"
     CDS             complement(586..891)
                     /locus_tag="VFA14_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 family protein"
                     /protein_id="HZS80573.1"
                     /translation="MTKSTYETVRNDMKTLVKDAQKLFREAAAATGDKADELRNKGLI
                     MLEAAVEAAQSAQAVAIQKSKEVAACTDNYVHENPWRAVAISAGIGLLVGLLIGRSR"
     gene            complement(944..1849)
                     /locus_tag="VFA14_03995"
     CDS             complement(944..1849)
                     /locus_tag="VFA14_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HZS80574.1"
                     /translation="MSDTSGPEVHQPNSRPPETPWGQTWKLLTARFRAMSDKAGRRIM
                     QRTLKIGISARIFHPESGAKGLRSKTLQYLEESIAQWVMSRDVLVFMIPTVNTNGRVH
                     HGNIRLRDYARHLDGLVLQGGADVSPHSYAEAVTRPEWNGDRVRDVYELELLHEFIEA
                     GKPVLGICRGCQLINVAFGGTLHQDIATDVPAAIPHISDLYDGNYHEVHFPPGSTLAS
                     LVNTQSAMVNSIHHQAVKDLGRDLSVEAVSSNDDMIEAIRYRKAPFVMGLQWHPEFHR
                     ADGPGLLDCMPILDSFLRTARETRF"
     gene            complement(1934..>2143)
                     /gene="rnpB"
                     /locus_tag="VFA14_04000"
     ncRNA           complement(1934..>2143)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VFA14_04000"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
BASE COUNT          481 a          651 c          560 g          451 t
ORIGIN      
        1 ccccgcaatc cggctcagcg cgcccttggc catggactgc gcatcgaggc tggcacgcac
       61 ggtactgcgc ccctccatca ggccgatgcg aaacaaggcc ccctcggcca gcagcctgcg
      121 cttgcgctct tctgatggaa cctcggactt ccagtctgtc gacgccatgg tttcctttta
      181 tttataacag cgcatcacgg tctttgcgca actccgtcat ggtcgcaggc aaagacaggc
      241 agccctgacg caaaatgggg cgcgcataca agatgatgag cgccgtgagc acggtaaaga
      301 aaatgccaaa cgcgacgaaa atcttccagc cccatgattc ccaggcaagg aaaaccacca
      361 gcgctgtcca gaatgccagc gcaaaccaca acgaaatcgc cgccagagtg aacaacacca
      421 aaaacctgac caggttgcga ccgatcccag agaactccag tgcagccagc tcgatacggt
      481 tgatcatcag accaagaaaa ttcctggcaa tacccagcaa cccggcgatc aggcccggat
      541 tgacaggctg ttcctgttca tgagccatgt cggtcccggc agcgcctacc tgctgcgacc
      601 aatgagcaga ccaaccagca agccgatgcc ggcagaaatc gccaccgcgc gccaaggatt
      661 ttcatgtaca tagttatcgg tacaggcggc gacctccttg ctcttctgga tagcaaccgc
      721 ctgagcgctc tgtgcggcct caaccgcagc ttccagcata atcaaacctt tattgcgtag
      781 ttcatcggct ttgtcaccgg tggcggcggc agcttcgcgg aacagtttct gcgcatcttt
      841 gaccaaagtt ttcatatcgt tcctcactgt ttcataggta gattttgtca tggaaattct
      901 cctttgcatg ccatgttcaa gcaagtgact gctcttgcac aactcagaaa cgcgtttccc
      961 gggcagtcct tagaaaactg tcgagaatcg gcatgcaatc cagcaatccg gggccgtcag
     1021 cgcgatgaaa ctccggatgc cattgcaagc ccatcacaaa cggcgccttg cgataacgga
     1081 ttgcttcgat catatcgtca ttactcgaca ccgcttccac cgacagatca cgccccagat
     1141 ccttcaccgc ctgatgatga atggaattga ccatggcgct ttgcgtgttg accagcgacg
     1201 cgagcgtcga gcctggtgga aaatgcactt cgtgatagtt gccgtcgtac agatcgctga
     1261 tatgcggaat cgctgccggc acgtcggtgg cgatgtcctg atgcagcgta ccgccaaagg
     1321 caacattgat caactggcag ccgcggcaaa tgcccagcac cggcttgccg gcctcgataa
     1381 attcatgcag cagttcgagc tcatacacat cacgcacccg gtcgccattc cactccggac
     1441 gcgtgacagc ctcggcatag ctgtgcggtg acacgtcggc gccgccctgc agcaccagcc
     1501 cgtccagatg cctagcgtag tcgcgcaggc ggatattgcc gtggtggacc cggccgttgg
     1561 tattgaccgt tgggatcatg aacaccaata catcgcgcga catcacccat tgcgcgatcg
     1621 actcttccag gtattgtaag gtcttgctac gtaagccctt ggcgccgctt tcgggatgga
     1681 agatgcgcgc ggagatgccg atcttgagcg tgcgctgcat gatacggcga ccggctttgt
     1741 ccgacatcgc cctgaaacgc gctgtcaaca gcttccaggt ttgcccccaa ggggtttccg
     1801 gtggccggga gttgggttga tggacttctg ggccgctcgt atcggacatg gcaaagttca
     1861 aagttctgta ggattgaaat caatgactgc aatcaggtgc atcccgattc aaacacaggg
     1921 gatccggata aaacagtgaa gcaagccgat aggccggatt ctgttacgac ggttgcccgt
     1981 tatgacagcc attcctctag gcgccggatt actccggcgc tcaagctttc tacccgcacg
     2041 ctctacgagc agcatcaatg cgtgcctatt tgaaattgct ccgggtggag gttaccgcgt
     2101 ttcaccgtaa ctcaatacgc tcgtctctgt ggccctgttc ctc
//
LOCUS       DASRDH010000175         3088 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_367357,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000175 DASRDH010000000
VERSION     DASRDH010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 3088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3088
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367357"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            127..1842
                     /gene="rpsA"
                     /locus_tag="VFA14_04005"
     CDS             127..1842
                     /gene="rpsA"
                     /locus_tag="VFA14_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HZS80575.1"
                     /translation="MSTITVLAPGAAGADAFAALFNESLSRQDMRSGEVISAEVVRLD
                     HNFVIVNAGLKSEAFIPVEEFKNDNGELEVNVGDFISVAIESLENGFGDTILSRDKAK
                     RLASWLSLEKAMESGEIVVGTVNGKVKGGLTVLTNGIRAFLPGSLVDTRPVKDTTPFE
                     GKTLEFKVIKLDRKRNNVVLSRRAAIEASMGEERQKLMETLKEGTVITGVVKNITDYG
                     AFVDLGGIDGLLHITDLAWRRVRHPSEVLTVGQEITAKVLKYDQEKNRVSLGVKQLGD
                     DPWTGLSRRYPQGTRLFGKVTNLTDYGAFVEVEQGIEGLVHVSEMDWTNKNIAPGKVV
                     QLGDEVEVMVLEIDEERRRISLGMKQCRQNPWDDFASSHRKGDKVRGAIKSITDFGVF
                     IGLSGNIDGLVHLSDLSWTEAGEEAVRRFKKGDELEAVVLAIDVERERVSLGVKQLEG
                     DPFSNFAVMNDKGALVTGIVKSVEPKGAVIQLSDEVEGYLRASEVSRDRVEDVGTHLK
                     IGDRVEALVINIDRKSRSIQLSIKAKDHAETQEAMQKMSADSNAAAGTTSLGALLKAK
                     LDNKN"
     gene            1856..2161
                     /locus_tag="VFA14_04010"
     CDS             1856..2161
                     /locus_tag="VFA14_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HZS80576.1"
                     /translation="MTKSELIARLAECYPQLVAKDADYAVKTILDAMVDALAGGQRVE
                     IRGFGSFALNRRPPRIGRNPKSGDKVMVPEKRVPHFKPGKELRECVDAMVGRSIEED"
     gene            2371..2664
                     /locus_tag="VFA14_04015"
     CDS             2371..2664
                     /locus_tag="VFA14_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HZS80577.1"
                     /translation="MKIMSRLIAVILFIFFFFFALRNMQEITLHFLFGYERTDPLVLV
                     LLVCFIGGAMFGVLAMTPIVFHHRREASRHKQTIAVLKKEREAHSVESSQHLV"
     gene            2668..>3088
                     /locus_tag="VFA14_04020"
     CDS             2668..>3088
                     /locus_tag="VFA14_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HZS80578.1"
                     /translation="MEFEFWWLLGIPIFFGLGWVAARVDISQLLAESRTLPRAYFKGL
                     NFLLNEQPDKAIDAFIEIVKLDPESIDLHFALGNLFRRRGETERAIRVHQNLLARPDL
                     LAEHKAHVQYELGQDYLKAGLLDRAEETFHLLKGTQYG"
BASE COUNT          695 a          776 c          885 g          732 t
ORIGIN      
        1 cgctgaatgc aaagttcagt gagtgttgac aaacctgaac ccattattta tcgctttttg
       61 cgcgcatcgg cagtttgatt ttcgcaaatc gtgaccggat actggtcaac acctttcttg
      121 atttttatgt ctaccattac tgtcctggct cccggcgccg ccggtgccga tgcatttgca
      181 gcgctgttca acgaatcgct gtcgcgtcaa gatatgcgtt ccggcgaagt catttcggcc
      241 gaagtcgtcc gcctcgacca taacttcgtt atcgtcaacg ccggcttgaa gtccgaagcc
      301 ttcattcctg ttgaagaatt caaaaacgac aatggcgaac ttgaggtcaa tgtcggcgat
      361 ttcatttctg tcgcaatcga atcgctggaa aacggtttcg gcgatacgat cctatcgcgc
      421 gacaaggcca agcgtctggc atcgtggctc tcgttggaaa aagcgatgga gtccggcgag
      481 atcgtcgttg gtactgtcaa tggcaaggtc aagggaggtt tgaccgtttt gaccaacggc
      541 atccgtgcct tcctgccggg ttcgctggta gatacgcgcc cggtcaagga tacaacgcct
      601 ttcgaaggca agaccctgga attcaaggtc attaagctgg atcgcaagcg caacaatgtc
      661 gtgctgtcgc gtcgcgccgc catcgaagcg tcgatgggtg aagagcgtca aaagctgatg
      721 gaaaccctga aagaaggcac tgtgattacc ggtgtcgtca agaatattac cgattatggt
      781 gcgttcgtgg acctgggtgg tatcgatggt ttgctgcata tcaccgatct ggcatggcgt
      841 cgtgtgcggc atccatcgga agtgctcacc gttggtcagg aaatcacggc caaggttctc
      901 aaatacgatc aggaaaagaa tcgtgtttcc ctgggcgtca aacagctcgg tgacgatcca
      961 tggaccggcc tgtcgcgtcg ttacccgcaa ggcacgcgcc tgttcggtaa agtgaccaat
     1021 ttgacagact acggcgcatt cgtcgaggtc gagcaaggta tcgagggtct ggtgcatgtt
     1081 tccgaaatgg attggaccaa caagaacatc gcgcctggca aggtggttca actgggcgac
     1141 gaagtggaag tcatggttct ggaaatcgac gaagagcgtc gccgcatcag tctgggcatg
     1201 aagcaatgta ggcaaaatcc atgggacgat tttgcgagca gtcacaggaa aggtgacaag
     1261 gtcaggggcg ccatcaagtc gatcactgac tttggcgtgt tcatcggcct gagcggcaac
     1321 atcgatggcc tggttcacct gtccgatctg tcctggaccg aagccggcga agaagctgtt
     1381 cgtcgcttca agaagggcga cgagctggaa gcagtcgtgc tggcgatcga tgtcgagcgc
     1441 gagcgggttt ccctgggcgt caagcaactg gagggcgacc cgttcagcaa tttcgccgtc
     1501 atgaacgaca agggcgcgct ggtgaccggt atcgtcaaat cggtcgagcc caagggcgcg
     1561 gtgatacagt tgtccgatga agtcgaaggt tacctgcgtg cttccgaagt ttcacgtgat
     1621 cgcgtcgaag atgttggcac acacctgaag ataggcgatc gtgttgaagc gctggtcatc
     1681 aatatcgacc gcaagtcccg cagcattcaa ttgtcgatca aggcaaagga ccatgctgaa
     1741 actcaggaag cgatgcaaaa gatgtctgcg gactctaatg ctgcggccgg caccaccagc
     1801 ctgggcgcat tgctgaaggc aaaactcgac aacaaaaact gatgcatcga gcgcgatgac
     1861 taagtctgag ctgatcgcgc gtctggccga gtgttatccg caacttgtcg ccaaggatgc
     1921 ggattacgcg gtcaaaacca ttctcgatgc catggttgat gcgctggcag gtggccagcg
     1981 tgttgagatc cgtggttttg gtagttttgc cttgaatcgc aggccgcctc gcattggccg
     2041 caatcccaaa tccggcgaca aagtgatggt gcccgagaag cgtgtgccac acttcaagcc
     2101 cggcaaggaa ttgcgggaat gtgtcgatgc catggtagga cgatccatcg aggaggactg
     2161 agaagtcgtt atgaactcat tcccggagca gctcaccaga cgtcgtgaga gttcgaatgc
     2221 agatgacgca gcatgcgacg agtgcgtgcg ctcggctctc ggcggtgacg cccgatgctt
     2281 cctttgcggg tgaggtgatt gaacggcatc tttggatgcc gtttttttca cgtacaatct
     2341 catgcccaat atcgcaaaag gattaacgcc atgaagataa tgtcaagact gattgcggtc
     2401 atcctcttta tttttttctt tttctttgcg ttgagaaaca tgcaggaaat cacgctgcat
     2461 tttctctttg gctatgaacg caccgatccc ttggtactgg tgttactggt gtgctttatc
     2521 ggcggcgcta tgtttggggt gctggccatg acgcctatcg tgtttcacca ccgtcgggaa
     2581 gcgtcgcgcc acaagcagac catcgctgtt ctgaagaaag aacgcgaagc ccatagtgtc
     2641 gaatcttccc agcatcttgt ctaaactatg gaatttgaat tctggtggtt gcttggcatc
     2701 ccgatatttt tcggcctggg ctgggtcgcg gcgcgtgtcg atatcagtca gttgcttgct
     2761 gaatcacgca ctttgccacg cgcttacttc aagggtttga acttcctgct caacgaacag
     2821 ccggacaagg cgatcgatgc tttcatcgaa atcgtcaagc tggatccgga aagtatcgat
     2881 ctgcactttg cgctgggtaa tctattccgt cgccgcggcg aaaccgagcg tgccatccgc
     2941 gtacaccaga atctgctggc tcgtcccgat ttgctggcgg aacacaaggc gcatgtgcaa
     3001 tacgaactgg ggcaggacta tctgaaggcc ggtttgcttg atcgcgctga agaaaccttt
     3061 catttgctca agggtactca atatggcg
//
LOCUS       DASRDH010000176         8435 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_574711,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000176 DASRDH010000000
VERSION     DASRDH010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 8435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8435
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_574711"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /gene="accB"
                     /locus_tag="VFA14_04025"
     CDS             <1..370
                     /gene="accB"
                     /locus_tag="VFA14_04025"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HZS80579.1"
                     /translation="AKSSATLPNQVVMMQPQAAYPQTVYAGAPVTASVAAPVAAAVPE
                     EGHIIKSPMVGTFYRSSAPGTPPFVEIGKEVKEGETICIIEAMKLLNEIDAAKAGVIR
                     QILVENGQPVEFGQPLFVID"
     gene            446..1825
                     /gene="accC"
                     /locus_tag="VFA14_04030"
     CDS             446..1825
                     /gene="accC"
                     /locus_tag="VFA14_04030"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HZS80580.1"
                     /translation="MFEKILIANRGEIALRIQRACREMGIKTVVVHSEADREAKYVKL
                     ADESVCIGPAPSALSYLNMPAIISAAEVTDAQAIHPGYGFLSENADFAERVEQSGFVF
                     IGPSAESIRMMGDKVSAKQTMIRAGVPCVPGSEGALPDNPKEIVQIARKIGYPVIIKA
                     AGGGGGRGMRVVHTEAALINAVTMTRSEAGTAFGNPEVYMEKYLEDPRHVEIQILADQ
                     HKNAIWLGERDCSMQRRHQKVIEEAPAPGIPRKIIEKTGERCAEACRKMGYRGAGTFE
                     FLYENEEFYFIEMNTRVQVEHPVTEMITGVDIVQEQIRIAANEKLRYRQRDIEFKGHA
                     IECRINAEDPFRFTPSPGRLTAWHVPGGPGIRVDSHAYAGYAVPPNYDSMVGKLISYG
                     ATREQAIRRMQVALSEMVVEGISTNIPLHRELMVDARFIEGGTNIHYLEHKLSERPDA
                     PTAVKPAKK"
     gene            1826..2734
                     /gene="prmA"
                     /locus_tag="VFA14_04035"
     CDS             1826..2734
                     /gene="prmA"
                     /locus_tag="VFA14_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HZS80581.1"
                     /translation="MAWTEIVMQVTRDRAEAWSDALLEAGALSVSVEDADAGTAAEQP
                     LFGEPGMQAAAWQRSHVAALVDNDANHAAIVNDAAKAIGLTYALPFTLRPVAKQDWVR
                     LTQSQCDPIPIGKRIWVVPSWHDAPEPDALVLALDPGLAFGTGSHPTTRLCMEWLEEH
                     AVDAATVLDYGCGSGILALVAKKCKAAHVVGVDIDPQALESARFNSKRNACHIDYYLP
                     EAFVRAYPENQTFAIVAANILAGPLQLMAPMLAGRVAPGGALLLSGVLDHQADAVIAA
                     YAPTIALKVWARHEGWIALTGRMPTA"
     gene            2772..3737
                     /locus_tag="VFA14_04040"
     CDS             2772..3737
                     /locus_tag="VFA14_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3426 domain-containing protein"
                     /protein_id="HZS80582.1"
                     /translation="MALATRCPSCRTTFRITQDQLELRNGLVRCGHCKEVFSGKQHLL
                     GPEITQQPAAAPMPAPAENQPVPTPTAPPWMAGTSLATEFARIAAETEDAPWSTPAKQ
                     PTPNALTQELPHPIPLTPSALPQAADKATVAPDETEKKAETGADDIPAFIHQAERRER
                     WRRTSRIIMLLGVVLLTITLLLQTIHVWHHTITAWLPATRPLLSSACAVLRCSTSLPA
                     NIAQLSLESAEMQLVPPHRNIYMLTVLLRNHSRHAQAWPHLELTLDDANEKTVIRRVF
                     APREYLRPIQQVDDGLPGASEQQIKLNFELAQPLASAYRVYLFFP"
     gene            3752..4687
                     /locus_tag="VFA14_04045"
     CDS             3752..4687
                     /locus_tag="VFA14_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008334666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HZS80583.1"
                     /translation="MSSLICGSLAYDNIMQYDRRFAEALLADQLHNINVSFLAPSMRR
                     EFGGCAGNIAYNLKLLGGEPLIMAVVGQDSLHYLERLRSLGISTKNIRVIDSAFTAQC
                     FIVTDIDNNQITAFHPGAMSFAHENKVADAGPVKFSIVAPDGRDAMLQHAEHSAALHI
                     PLIFDPGQNFPLFNGDELKHFIDLATYITVNGYEAESLAARTGLSLEQIAGQVSALII
                     TRGALGAEIFTNGQKIDIPCVPAETIADPTGCGDAFRAGILFGLNKGMDWTTTGRLSS
                     LMGSIKIASQGPQNHAPTLAEIDARFHQAFGYRFT"
     gene            4744..5289
                     /locus_tag="VFA14_04050"
     CDS             4744..5289
                     /locus_tag="VFA14_04050"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013232429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HZS80584.1"
                     /translation="MKHLASRLMISLFILSAGPAALAALKPGDKAPDFSAQASLGGQI
                     TPFSLATALKKGPVVLYFYPAAFTTGCTIEAHLFAEAIDRYEALGATVIGISRDSIDT
                     LNKFSVSACRSKFAVAADADQKIMTSYDALMPQSGHASRTSYVIAPDHTIIYEYSSPS
                     PDRHAENTLQALQQWVSSQKK"
     gene            complement(5301..5834)
                     /locus_tag="VFA14_04055"
     CDS             complement(5301..5834)
                     /locus_tag="VFA14_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HZS80585.1"
                     /translation="MLHSIFMLVVDTVASVLAGTLLLRFWMQAVRVRPPASVGQFVLH
                     LSDWLVQPLRRVLPGSAGYDWASLAGAFLVALLSVIAASGFMLGSEIVLLSLVRFFQW
                     IFYGLIITLIIEAIFSWLHPDAPLAPFVRALNRPLLRPLRSVIPLIGNVDLSPLAALL
                     LLQIALRLLSSLLLSIG"
     gene            complement(6107..6337)
                     /locus_tag="VFA14_04060"
                     /pseudo
     CDS             complement(6107..6337)
                     /locus_tag="VFA14_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006400402.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like protein 2"
     gene            6385..6531
                     /locus_tag="VFA14_04065"
     CDS             6385..6531
                     /locus_tag="VFA14_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS80586.1"
                     /translation="MIQATRLHHRDNANGLFIGTGKLLLVLMNPAPAELLHPLTMQHF
                     SYLG"
     gene            complement(6669..7736)
                     /locus_tag="VFA14_04070"
     CDS             complement(6669..7736)
                     /locus_tag="VFA14_04070"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013232426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HZS80587.1"
                     /translation="MLSWKDEVKEAPAPYAASRVDIADKRIINGHTDVNQLVPFKYKW
                     AWDKYLAGCANHWMPQEINMQRDIELWKNPNGLTEDERRLVKRNLGFFVTADSLAANN
                     IVLGTYRHITAPECRQYLLRQAFEEAIHTHAYQYIVESLGLDEAEIFNAYHEVKSIRD
                     KDEFLIPFINTLTDPQFRTGTTENDQKLLKSLIVFACIMEGLFFYVGFTQILALGRQN
                     KMTGAAEQYQYILRDESMHCNFGIDLINTVKMENPHLWTPEFRNDIKSLFSSAVELEY
                     RYAEDTMPRGVLGLNAPMFKAYLRFIANRRAQQIGLDPLFAQEENPFPWMSEMIDLKK
                     ERNFFETRVIEYQTGGALNWE"
     gene            complement(7839..>8435)
                     /locus_tag="VFA14_04075"
     CDS             complement(7839..>8435)
                     /locus_tag="VFA14_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZS80588.1"
                     /translation="FRLVTATNRNLRELVRQEMFRSDLYYRMAVIELRVPNMEERGAI
                     DKVAIFTSVVTRLLGAAGSEAREQAAPPEIPDWLLQLIAQTRFNGNVRELRNIAERIA
                     IIHRQFGDWDRDAIERVFSLDRDSRMEDDGSDEQGKKWGSADHSDRARIIEALNAHNW
                     RRQDTAHYLNISRKVLWEKMRKLQIIDGDQALAGESEP"
BASE COUNT         1911 a         2649 c         2225 g         1650 t
ORIGIN      
        1 cgccaagtca tcagcaaccc tgccaaacca ggtcgtcatg atgcagccgc aagccgctta
       61 tccgcaaacc gtgtacgctg gcgctcccgt cacggcgtcg gtggcggcac ccgtcgcagc
      121 tgccgtaccg gaagaaggcc atatcatcaa atcgccgatg gtcggcacct tctaccgctc
      181 ttccgcgcca ggtacgccgc cattcgtcga aatcggcaaa gaagtcaagg aaggcgaaac
      241 catctgcatc atcgaagcca tgaaactgct caacgaaatc gacgccgcca aggccggtgt
      301 gatcaggcag atactggttg aaaacggtca accggtcgaa tttggccaac cgctgttcgt
      361 gatcgactaa atcctgccag aatacagatg attactgaat acaccatccc gcgcgcttga
      421 ctctcgacga tgcatatttc ttcttatgtt tgaaaaaatc cttattgcca atcgcggcga
      481 aatcgctctc cgtatccagc gcgcatgccg tgaaatgggc atcaagaccg tagtcgtcca
      541 ctccgaagcg gaccgcgaag cgaagtacgt caagctggcg gatgaatcag tctgtatcgg
      601 cccggctcct tcggcgttga gctacctgaa catgccggcc atcattagcg ccgccgaagt
      661 caccgatgcc caggcgatcc atcccggcta cggctttctg tccgaaaacg ccgatttcgc
      721 cgagcgcgtc gaacagtctg gcttcgtctt catcggccca tctgcggaaa gcatacgcat
      781 gatgggcgac aaggtctcgg ccaaacagac catgatccgc gccggcgtgc cttgcgtacc
      841 cggctcggaa ggagcattac cggataatcc gaaagaaatc gtgcagatcg cgcgcaagat
      901 cggctacccg gtcatcatca aggcagccgg cggcggcggc ggtcgcggca tgcgcgtggt
      961 gcataccgaa gcggcgctga tcaacgccgt taccatgacc aggagcgaag ccggcaccgc
     1021 tttcggcaac ccggaagtct atatggaaaa atatctggaa gatccacgtc acgtggaaat
     1081 tcagatcctc gccgaccagc acaaaaatgc gatctggctg ggcgaacgcg actgctcgat
     1141 gcagcgccgg caccagaagg tgatcgaaga agcgccggcg ccaggtatcc cgcgcaagat
     1201 catcgaaaaa actggtgagc gctgcgctga agcctgccgc aagatgggct atcgcggcgc
     1261 cggcaccttc gaattcctgt acgaaaacga agaattctat ttcattgaaa tgaatacccg
     1321 cgtgcaggtc gaacatccgg tcacagaaat gatcaccggc gtcgacatcg tgcaggaaca
     1381 gatccgcatc gcggccaacg aaaagctgcg ctatcgccag cgcgacatcg agttcaaggg
     1441 gcatgccatc gagtgtcgca tcaacgccga agatccgttc cggttcacac cctcgccggg
     1501 tcgcctgacc gcctggcatg tgccgggcgg ccccggcatc cgtgtcgact cgcatgccta
     1561 cgccggctat gccgtcccgc cgaactacga ctccatggtt ggcaaactga tctcctacgg
     1621 cgccacacgc gaacaggcga tccgccgcat gcaggtcgcg ttgtcggaaa tggtggtgga
     1681 aggcatttcc accaatatcc cgctgcaccg tgaactgatg gtcgatgccc gcttcatcga
     1741 aggcggcacc aacatccatt acctcgaaca caagctgtcg gagcgcccag acgcgccaac
     1801 agccgtcaaa ccagcgaaaa agtagatggc ctggaccgaa atcgtcatgc aagtcacgcg
     1861 cgaccgggct gaagcatggt ctgatgccct gctggaagcc ggtgcgctgt cagtctcggt
     1921 ggaagacgcc gacgcaggca ccgccgccga acagccactg ttcggcgaac ccggcatgca
     1981 agcggctgca tggcaacgca gccacgtggc ggcattggtc gataacgacg ccaaccatgc
     2041 cgccatagtc aatgacgccg ccaaggccat cggtctgaca tatgcgctgc cgttcacttt
     2101 gcgcccggtg gcgaaacaag actgggtgcg cctgacgcaa tcgcaatgcg atcccatccc
     2161 tatcggcaaa cgcatctggg tcgtgccaag ctggcatgac gcgccggaac ccgacgccct
     2221 ggtactggcg ctggacccgg gtctggcctt cggtaccggc agccatccga ccacacgcct
     2281 gtgcatggaa tggctggaag aacacgccgt cgatgctgcc acggtactcg actatggctg
     2341 cggctccggc atcctggcgc tggtggcaaa aaaatgcaag gcggcgcacg tcgtcggcgt
     2401 ggacatcgac ccgcaagcgc tggaatcggc gcgcttcaac agcaaacgca acgcctgcca
     2461 catcgactat tacctgccgg aagccttcgt ccgcgcatat ccggaaaacc agaccttcgc
     2521 catcgtggct gccaatattc tggccggacc gctacaactg atggcaccga tgctggctgg
     2581 ccgcgtagcg ccgggcggcg cgctgctgct atcgggcgtg ctggatcatc aggctgacgc
     2641 agtcatcgcc gcctacgcgc ccactatcgc gctcaaggtc tgggccaggc acgaaggctg
     2701 gatcgcgctg accggccgga tgccgaccgc atgatcatgc agcgcggcat cgaacctgat
     2761 ggagtatgct aatggcgctg gcgacccgat gcccttcctg ccgaacgaca ttccgcatca
     2821 ctcaagacca gctcgagcta cgtaacggcc tggttcgctg tggtcattgc aaggaagtct
     2881 tcagtggcaa acagcacctg ctcggaccgg aaatcacgca gcaaccagca gcggcaccga
     2941 tgccggcgcc ggcagaaaac cagcctgtgc caacgcccac ggcgccaccc tggatggcgg
     3001 gcaccagtct ggcgacagaa tttgcccgca tcgcggcaga aaccgaggat gctccctgga
     3061 gtacgccggc aaaacagcca acgcccaatg ccctgacaca ggaattgccg catccgattc
     3121 cactcacgcc atcagcactg ccacaagcgg ctgataaagc aacggtcgcg ccagacgaaa
     3181 cagaaaaaaa agcggaaaca ggcgcggacg acatacccgc cttcatccac caggccgagc
     3241 gccgggaacg ctggcgccgg accagccgca tcatcatgct cctcggcgtg gtattgctga
     3301 cgattacctt gctgctgcaa accatccatg tctggcacca caccattacc gcctggctgc
     3361 ccgcaacacg gccgctgctg tcgtcggcct gcgcggtcct gcgctgttcg accagcctgc
     3421 ccgccaatat cgctcagttg tcactggaat cggccgagat gcaactggta ccaccccacc
     3481 ggaatatcta catgctgacg gtgctgctgc gcaatcacag ccgccatgcc caggcctggc
     3541 cccacctgga actgaccctc gacgacgcca acgaaaaaac cgttatccgc cgcgtctttg
     3601 ccccacgtga atatctgcgc ccgatacaac aggtcgacga cggcctgccc ggcgcaagcg
     3661 agcagcagat caaactgaac ttcgaactgg cgcaaccact cgcttcagct tatcgcgtct
     3721 atctattctt tccttgaacc gctgccccga catgagttca ctgatttgcg gctccctcgc
     3781 ctacgacaac atcatgcaat acgatcgccg ctttgccgaa gcgctactgg ccgatcaatt
     3841 gcacaacatc aatgtgtcct ttctggcacc atcgatgcgc cgcgaattcg gcggttgcgc
     3901 cggcaacatc gcctataacc tgaaactgct cggcggcgaa ccgctcatca tggccgtcgt
     3961 cggccaggac agcctccact acctggagcg cctcaggagt ctcggcatct ccacaaaaaa
     4021 catccgcgtc atcgacagcg ctttcaccgc gcaatgtttc atcgtcaccg atatcgacaa
     4081 taatcagatc accgcattcc accccggcgc catgagcttc gcgcatgaga acaaggtcgc
     4141 cgacgccggt ccggtcaagt tttccatcgt cgcgccggac ggccgcgacg ccatgctgca
     4201 acatgcagaa cacagcgccg cgctgcatat cccgctgatt ttcgacccag gccaaaattt
     4261 tccattgttc aatggcgacg agctcaaaca tttcatcgat ctggccactt acatcactgt
     4321 caacggctac gaagccgaat cgctggccgc gcgtaccggc ctgagcctgg agcagatcgc
     4381 cgggcaagtc tcggcgctga tcatcacgcg cggagcgctg ggtgcggaaa tcttcaccaa
     4441 cggccaaaaa atcgacattc cctgcgtgcc cgccgaaacc atcgccgacc cgaccggctg
     4501 cggcgacgcc ttccgcgccg gtatactgtt cggcctgaac aaaggcatgg actggaccac
     4561 cacaggacgt ttgtccagct tgatgggttc aatcaagatt gcctcgcaag gtccacaaaa
     4621 ccatgcgccg acactggctg aaatcgatgc acgattccat caggctttcg gctaccgttt
     4681 cacctgatcg cctggatcca cccacggcgt tttgcagcaa ttcatgtatt gaaaggatgg
     4741 actatgaaac acctcgcttc cagactcatg atttcgttgt tcatcctgtc ggcaggacca
     4801 gcggcactgg ccgcgctcaa gcccggcgac aaagcaccgg atttctcggc gcaggcatcg
     4861 ctgggcggcc agataacacc cttctcgctg gccacagcgc tgaagaaagg acctgtcgtg
     4921 ctgtacttct atcctgccgc cttcaccact ggctgtacca tcgaagccca tctgttcgcc
     4981 gaagcaatcg accgttacga ggcgctcggc gccactgtca tcggtatctc ccgcgacagc
     5041 atcgacaccc tcaacaaatt ttccgtcagc gcatgccgca gcaaatttgc cgtggcagcc
     5101 gacgccgacc agaagatcat gacatcctat gatgcgctca tgccacagtc cggccatgcc
     5161 agccgcacat cctacgtgat cgcgccggac cacaccatca tttacgaata cagcagtccc
     5221 agcccggaca ggcacgccga aaacacgtta caggcgctcc agcaatgggt aagtagccag
     5281 aaaaaataac gggagaacgg tcaaccgatg gacagcagca gggagctgag tagacgcagg
     5341 gcaatctgca acagaagcag cgctgccaga ggtgacagat cgacgttgcc gatcagcgga
     5401 attacactac gcaaagggcg cagcaaaggt ctgttcaagg cacgcacgaa cggcgccagc
     5461 ggcgcatcag gatgcagcca actgaagatg gcttcgatga tcaacgtgat gatgaggcca
     5521 taaaaaatcc actggaaaaa gcgtaccagc gacaacagaa cgatctcaga ccccaacatg
     5581 aatccggacg ccgcaatgac cgacagcagc gccaccagaa atgcaccggc cagactggcc
     5641 cagtcgtaac cggcgctccc cggcaacacc ctgcgcagcg gctggaccag ccagtccgac
     5701 aagtgcagca cgaactgtcc caccgatgcg ggtgggcgta cccgcacggc ctgcatccag
     5761 aaacgcagca acagcgtgcc tgccagcaca ctggcaacgg tatcaacgac caacatgaaa
     5821 atactatgca acacgctcac tctccataat aagccatacg attgcatcgg gtacgatgcg
     5881 caggggcata tactaaacca tgcggcctgt ttccttgcgg catgcaaaac aaggtgagcg
     5941 aaaaacgggt acggcttccc atctgctgaa ccgcagtcag cagcaactgg atctgcaaag
     6001 aaggaagtgt tcgcgttgaa caaagcagac acacaaaaag accgctgcat gacaaggtca
     6061 cgcagcggta tcgcgtccgg ccaccagggc gcttggcccg gtctgattat ggacgtgctg
     6121 taccggtcgg aaacggccag gctgctgcct ggtttagcac agtcttcacc accgacgtta
     6181 ctgctttttt cactaccggc tttttggccg cgacggtttt tttcttggcc gccgtcgctt
     6241 ttttcgctac cggcttcttg gccgcgacgg tttttttctt gaccgccgtt gctttcttcg
     6301 ctgccggctt cctgggcgcc gctgctttct tcgctgctgt tgccattgta tttctccttt
     6361 ttcacatgat ggaaaaaatc aagcttgatt caagcaaccc gtctgcatca tcgcgataat
     6421 gcgaacggat tattcatcgg cacaggcaaa cttctgcttg tgctaatgaa tccggcacca
     6481 gctgaactgc tgcaccccct caccatgcag cacttcagct atcttggcta agccctctgc
     6541 cccattcagg ggcacgacac cgctactaaa aaaaacgcca gcgaatcaca cgctagcgcc
     6601 ttcggaaaac ctcaggtttt tcacctgacc cggtcatgac ggtgatgaaa cagtacagga
     6661 caggcacttc actcccagtt gagtgcgcct ccggtctggt actcgatgac acgagtttca
     6721 aagaaattgc gttccttctt cagatcgatc atctcgctca tccacggaaa cggattttct
     6781 tcctgcgcaa acagtggatc aagtccgatc tgctgcgcgc gacgattagc gatgaagcgc
     6841 aagtaagctt tgaacatcgg tgcattcaaa cctagcacac ctcgcggcat tgtatcctct
     6901 gcgtagcgat attccaactc cactgcgctt gaaaacaacg atttaatgtc gtttctgaat
     6961 tccggtgtcc acaaatgcgg attttccatt ttcaccgtgt tgatcagatc gatcccgaaa
     7021 ttgcaatgca tcgattcatc acgcaggatg tactggtact gttcagccgc acccgtcatc
     7081 ttgttctgac ggccgagcgc caggatctgc gtgaagccaa catagaagaa caggccctcc
     7141 atgatgcagg caaaaacgat cagcgatttc agcagcttct ggtcgttttc agtggtgcca
     7201 gtgcgaaact gcggatcggt cagtgtgttg atgaaaggaa tcagaaattc atccttgtcg
     7261 cgaatcgact tcacctcatg ataggcgttg aaaatttcag cttcgtccag accgagcgac
     7321 tcgacgatgt actggtaagc gtgcgtgtgg attgcttctt cgaacgcctg gcgcagcagg
     7381 tactgacgac attccggtgc cgtgatgtgt cggtaggtgc cgagtacgat attgttggcg
     7441 gccaacgaat cggcggtgac gaagaaaccg aggttgcgct tgaccagacg gcgctcgtct
     7501 tcggtcaggc cattcggatt tttccacagc tcgatgtcgc gctgcatatt gatttcctgc
     7561 ggcatccagt ggttggcgca accggccagg tacttgtccc aggcccattt atatttgaac
     7621 ggaaccagct ggttgacgtc ggtatgacca ttgatgatgc gcttgtcggc gatatcgaca
     7681 cggctggccg catatggcgc tggcgcttcc ttcacttcat ccttccagga gagcatgttg
     7741 atcctttacc tgattgactg atactgcgga tgagaacggt ttgcccattc tctccaagaa
     7801 gctctcatta tatagacaat atcggcgctc attttgcatc atggttcgct ctccccggca
     7861 agtgcctggt cgccatcgat gatctggagc ttgcgcattt tttcccataa caccttgcgg
     7921 ctgatgttca gataatgcgc ggtatcctga cgccgccagt tgtgcgcatt gagcgcttcg
     7981 atgattctgg cgcggtcact gtgatcagca ctgccccact tttttccttg ctcgtcacta
     8041 ccatcatctt ccatgcggct gtccctgtcc aggctgaaca cacgctcgat ggcgtccctg
     8101 tcccagtcgc cgaactggcg atgaatgatc gcgatgcgtt ctgcgatatt gcgtaattca
     8161 cggacattac cattgaaccg cgtctgcgca atcaattgca gcagccaatc gggaatctct
     8221 ggcggcgcgg cctgttcgcg cgcctcactg ccggcggcgc cgagcaggcg cgtcacgacg
     8281 gaggtaaaaa tagccacctt gtcgatcgcg ccgcgttctt ccatattggg aacgcgtaat
     8341 tcgatcaccg ccatccggta atacagatca gagcggaaca tctcttgccg caccagttcc
     8401 cgcagattgc ggttggtggc ggtcaccagc ctgaa
//
LOCUS       DASRDH010000177         2420 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_702390,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000177 DASRDH010000000
VERSION     DASRDH010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_702390"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /gene="gidA"
                     /locus_tag="VFA14_04080"
     CDS             <1..747
                     /gene="gidA"
                     /locus_tag="VFA14_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008119100.1"
                     /note="GidA; glucose-inhibited cell division protein A;
                     involved in the 5-carboxymethylaminomethyl modification
                     (mnm(5)s(2)U) of the wobble uridine base in some tRNAs;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HZS80589.1"
                     /translation="GILAGINAALQTQGDDSWTPRRDEAYLGVLVDDLITQGVQEPYR
                     MFTSRAEYRLSLREDNADLRLTEIGRRLGCVSDAQWEMFEKKREALAREMERLKFTWI
                     NPRILADVEAERVLGKTIEREYNLMDLLRRPNVSYDSLMTLTGTDGQALAGPGVTDDL
                     IREQIEIQIKYAGYIDRQAKEISRQEHNENLKLPSDLDYMEVKALSIEVRQKLNRHKP
                     ETLGQASRISGVTPAALALLLVHLKKGKLN"
     gene            756..1406
                     /gene="rsmG"
                     /locus_tag="VFA14_04085"
     CDS             756..1406
                     /gene="rsmG"
                     /locus_tag="VFA14_04085"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HZS80590.1"
                     /translation="MTIDDKLTDTLSDGIKTLGLTLSTSQQKKLLAYLALMIKWNHTY
                     NLTALRDPAQMMTHHLLDALAAVKVFQDATNVLDVGSGGGLPGIVLAIWAAEACPLMH
                     VSMIDTVRKKTAFLTQVKAELQLSNISIYTARIEQLQLEQRFDVITARAFSGLDDLVN
                     WSQHLLQTGGYYIAMKGIIPRDEIARLPDGWRVQQAKALQVPGLDAERHLIFIERC"
     gene            1413..2183
                     /locus_tag="VFA14_04090"
     CDS             1413..2183
                     /locus_tag="VFA14_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZS80591.1"
                     /translation="MAKIFCVANQKGGVGKTTTAINLAAGLAQHDQRVLLADLDPQGN
                     ATTGAGIDKSALQESIYEVLLGVSGIKNVRKRSESGNFDVLPANRELAGAEIEMVGLD
                     NREKRLKDAFAVVTDDYDFILIDCPPALSLLTLNGLCSAHGVVIPIQCEYYALEGLSD
                     LVNTIKKIHAKLNPKLEITGLLRVMFDPRLTLSQQVSEQLERHFGNKVFKTVIPRNVR
                     LAEAPSYGMPGVNFDTASRGAQAYIAFGAEMVERIKAL"
     gene            2214..>2420
                     /locus_tag="VFA14_04095"
     CDS             2214..>2420
                     /locus_tag="VFA14_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013236695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HZS80592.1"
                     /translation="MATRKSKGLGRGLDALLGGSSDMAEDVPVTSGSPSMIPITDLQA
                     GKYQPRSRMDEGALNELAASIKEQG"
BASE COUNT          617 a          628 c          627 g          548 t
ORIGIN      
        1 ggcatattag ctggtatcaa tgccgcgctg caaacgcagg gtgatgattc ctggacgcca
       61 cgccgtgatg aagcttatct tggcgtgctg gtggatgatt tgatcaccca gggtgtgcaa
      121 gaaccttacc gcatgtttac cagtcgtgct gaatatcgtc taagtctgcg tgaagataac
      181 gctgatctac gtctgaccga aatcggccgc aggcttggtt gcgtcagcga tgcccaatgg
      241 gagatgttcg aaaaaaaacg cgaagcgctt gcacgtgaaa tggaacgcct gaaattcacc
      301 tggatcaatc cacgcatcct cgccgatgtc gaagctgaac gtgtactggg aaaaaccatt
      361 gaacgcgaat acaatctgat ggatttactg cgtcgtccga acgtgagcta tgatagtctg
      421 atgacgctga ccggcaccga tggccaagcc ctcgccggtc cgggcgtcac tgacgatttg
      481 atacgcgaac agatagaaat ccagatcaaa tatgccggct acatcgaccg ccaggccaag
      541 gaaatcagtc gccaggaaca caatgaaaac ctgaaactgc catccgacct cgactatatg
      601 gaagtcaaag cactgtcgat cgaagtacgt cagaagctaa acagacacaa accagaaacc
      661 ctgggccagg cttcacgtat ttctggtgtg acgccagcag ccttggcgct gttattggtg
      721 catttgaaaa aaggcaagct gaattgaccg tccccttgac catcgacgac aaactgaccg
      781 acacgctcag tgacggtatc aaaacgctcg gactgacact gagcaccagc cagcaaaaga
      841 agctgctggc gtatctcgca ttgatgatca agtggaatca tacctacaat ctgaccgcac
      901 tgcgcgatcc ggcacagatg atgacgcatc atctgctcga tgcgctggca gcggtcaagg
      961 tttttcagga cgccaccaat gtactcgacg tcggttctgg cggtggtttg ccgggtatcg
     1021 tactcgctat ttgggcggct gaggcatgtc ccctcatgca tgtgtcgatg atagataccg
     1081 tgcgtaaaaa aacggctttc ttgacgcaag tcaaggcaga gttgcaatta agcaatattt
     1141 ctatttatac ggcacgtatc gaacagttac aacttgagca acgattcgat gtcatcaccg
     1201 cgcgcgcttt ttctggactc gacgacctcg tcaattggtc tcagcatctc ttgcagaccg
     1261 gtggttacta tatcgccatg aagggtatca ttccacggga cgaaattgcg cgtctgccgg
     1321 acggatggag agtccagcag gccaaagctc tacaggtgcc aggtctcgat gcggaacgcc
     1381 atctgatttt tatcgaaaga tgttgagtgt ctatggctaa aatcttttgt gttgcgaatc
     1441 aaaaaggtgg tgtcggcaaa accaccaccg caatcaatct cgccgccggt ctagcgcaac
     1501 atgaccagcg cgtgttgctt gctgaccttg atccgcaagg caatgccact accggcgctg
     1561 gtatcgacaa atcggcgctg caagagtcga tttatgaagt tttgctcggc gtgtccggca
     1621 tcaagaatgt acgcaagcgt tctgaaagtg gcaacttcga cgtactgccg gccaaccgcg
     1681 aactggccgg cgctgaaatc gaaatggtcg gtctggacaa ccgtgaaaag cgcttgaagg
     1741 atgccttcgc cgtcgttact gatgattacg atttcattct tatcgattgt ccgccggcct
     1801 tatcgctgct gacactcaat ggcctgtgct cagcgcatgg cgtagtgatc ccgatacagt
     1861 gcgaatacta tgcactcgaa gggctgtccg accttgtcaa taccatcaag aaaattcatg
     1921 ccaagcttaa tcccaaactc gaaatcaccg gcttgctgcg cgtgatgttc gaccctcgtt
     1981 tgactttgtc gcagcaggtt tccgagcagc tcgaacgaca tttcggcaac aaagtgttca
     2041 aaacggtgat cccacgtaat gtccgtcttg cggaagcacc gtcttacggt atgccaggtg
     2101 tcaatttcga taccgcatcg aggggcgccc aagcctatat tgcattcggc gcggagatgg
     2161 ttgaacgcat caaagcactt taaatttttt aatcattgac cgataggtga aagatggcaa
     2221 ccagaaaatc caaaggactt ggccgtggtc tcgacgcact gctcggcgga tcttccgaca
     2281 tggccgaaga cgtgccagtg acctctggtt cgccatcgat gataccaatc acggatctgc
     2341 aagcgggtaa ataccagcca cgtagccgca tggacgaagg cgcattgaat gaactggctg
     2401 cctcaatcaa ggaacaaggt
//
LOCUS       DASRDH010000178         4145 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_743712,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000178 DASRDH010000000
VERSION     DASRDH010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 4145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4145
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_743712"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1055
                     /locus_tag="VFA14_04100"
     CDS             <1..1055
                     /locus_tag="VFA14_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006712651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZS80593.1"
                     /translation="TNNLDIDTIRWLENVLNERNSTMIIISHDRHFLNQVCTHMADMD
                     YGTLKIYPGNYDDYMLASSQARAQQLAVNAKAKEKVAELQDFVRRFSANKSKARQATS
                     RARQIDKIKVEDVKPSSRVHPFIRFDGEKKLHRLAVETQGIGKTYDRPIFRNFSIMVE
                     AGEKIAIIGANGIGKTTLLRSIGGDITGLHPDSGLVKWAENANVGYMPQDPTEEFAGG
                     ETLIDWMRQWIQEGDDDQTIRSTLGRLLFSGDDVKKSVKVLSGGEKGRMIYGKLMLGR
                     HNILLMDEPTNHMDMESIESLNIALEKYAGTLVFMSHDREFVSSLATRILEVKENGII
                     DFQGSYEDYLASQGIQ"
     gene            complement(1122..3362)
                     /locus_tag="VFA14_04105"
     CDS             complement(1122..3362)
                     /locus_tag="VFA14_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019448678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HZS80594.1"
                     /translation="MSWAALLAGLLLPCAHAADNGADAASRPRIGLVLSGGGARGYAH
                     LGVLQYLEELRVPVDYIAATSMGALIGGLYASGISAQELERRLLHMNLNDIAFDRSER
                     AELPQSQREDDFQYPISIAAGYDDGQLKLATGLVQGNNLLALLQNWTAQLQADISFDL
                     LPIPFRAVATDLGQGTEVILDKGSLPRAMRASMAVPGLFAPYQIDGRILVDGGLVSNL
                     PVQEARGMGADIIIAVNIASPLHDPATLQSPPAVLQQMIGILIQQNVKAQKTLLGEHD
                     ILIEPELSGMSFADFARGKDGIKAGWEAAQAQSGRLAELSLSPQQWQAYLTSRHHSVT
                     LPRDIHIDAIEINNTGRIPASYVRSRLNIKEGDVYDADSLNQQLSQLSTNGDFNALTQ
                     EMLSKGGRNVLRIDVEEKSWGPQYLLFGLGISNTFDGHGNVNLQIGHRYPWMTQTGLE
                     WRSDLILGNESTSLHSELRQPIYGASGTFGVYMSPYIDIGRRFVDAYLDGSDARASPA
                     TQYQIDTGVIGFDFGMPLARLGDLRLGVSYQRTAYTPTYNFTSNSHASQFNDSVSSQP
                     VARARLTIDQLDDPLFPRRGYYIYSELDHGLGQVDERYGAAQLKTLWAFSHERDTVNM
                     ALEGAGNLSRNNSGPGFFLGGFQRLSAYAADQFYGNYLLYSRLTYLRNLEDDSLPQLR
                     HPVLGTSLEIGNVWQARAAFGNGPYKKSLNLFLGGNSPIGPLYFGTALGQQGVWNLYL
                     QLGRVF"
     gene            complement(3569..>4145)
                     /locus_tag="VFA14_04110"
     CDS             complement(3569..>4145)
                     /locus_tag="VFA14_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007877576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HZS80595.1"
                     /translation="VRALQQDGSIATHFCEPIGHIQVQGDYVESWQPHPMHPVALEKA
                     CDIARKVTDDLGGLGLFGVELFVKGDMVWFSEVSPRPHDTGMVTMITQIQSEFELHAR
                     AILGLPVNVTLRTPGASAVIYGRHDAKAIAFDGVADALRIPGADIRLFGKPESFARRR
                     MGVVLAVADDIDDARIRAKQAAAKVKPVKTV"
BASE COUNT          943 a         1200 c         1194 g          808 t
ORIGIN      
        1 cgaccaataa cctcgacatc gataccatcc gctggctgga aaacgtactc aacgagcgca
       61 actccaccat gatcatcatt tcccatgatc gtcatttcct caatcaggtc tgcacacaca
      121 tggccgacat ggactacggc acgctgaaaa tctatccggg caactacgac gactacatgc
      181 tggcatcatc gcaggcgcgc gcgcagcaac tggcggtcaa cgccaaggcc aaggaaaagg
      241 tcgccgaact gcaagacttc gtgcgccgct tctcggccaa taagtccaag gcacgtcagg
      301 cgacatcgcg cgccagacag atcgacaaga tcaaggtcga agatgtcaag ccatcgagcc
      361 gcgtccatcc cttcattcgc ttcgacggcg agaaaaagct gcaccgtctg gcggtggaaa
      421 cgcaaggcat aggcaagact tacgaccgtc cgattttcag gaacttcagc atcatggtcg
      481 aagccggcga aaaaattgcc atcatcggcg ccaatggcat cggcaagact acgctactgc
      541 gcagcatcgg cggcgacatc accggcctgc atcccgacag cggcctggtc aagtgggcgg
      601 aaaacgccaa cgtcggctac atgccacagg atccgactga agagttcgcc gggggcgaga
      661 ccttgatcga ctggatgcgt caatggatcc aggaaggaga cgatgatcag acgatacgtt
      721 cgacgctcgg ccgcctgctg ttctccggcg acgacgtcaa aaaatcggtc aaggttctgt
      781 caggcggtga aaaaggacgc atgatatacg gcaagctgat gctgggccgt cataacattc
      841 tgctgatgga cgagccgacc aaccacatgg acatggaatc aatcgagtcg ctcaatattg
      901 cccttgaaaa atatgccggc acgctggttt tcatgtcaca tgaccgtgaa ttcgtatcgt
      961 cactggccac acgtattctg gaagtgaagg aaaacgggat catcgatttc cagggcagct
     1021 acgaagacta tctggccagc cagggtatcc agtaatttgt atcgatcatt ccttggggtc
     1081 attgactgaa gcggcgcggc gctcggaacg tattcacgat ctcaaaatac gcgccccagt
     1141 tgcaaataca gattccacac accctgctga cccagcgccg tgccgaagta aagcggcccg
     1201 atcgggctgt tgccaccaag gaacagattc aggcttttct tgtaaggacc gttgccgaag
     1261 gcagcacgcg cttgccagac attaccgatt tccaggctgg tgccgagtac cggatgacgc
     1321 agttgcggca agctgtcatc ctcgaggttg cgcaaatagg tcaaacgact gtacaacaga
     1381 taattaccgt agaactgatc cgcggcataa gccgacaaac gctggaatcc accgagaaag
     1441 aagccagggc cgctattgtt gcgtgataga ttgccagcgc cttccagcgc catattgacc
     1501 gtgtcgcgtt cgtggctgaa tgcccacagc gttttgagct gagcagcgcc atagcgttcg
     1561 tctacctgac cgagcccgtg atcgagctcc gagtaaatgt aatagcctct gcgtggaaac
     1621 agcggatcgt cgagctgatc gatggtcagc cgggcgcgtg cgaccggctg gctggagacc
     1681 gagtcattga actgcgaagc atggctgtta ctggtgaaat tgtaagtcgg cgtatacgcc
     1741 gtgcgctgat agctcacgcc cagtcgcaag tcacccagcc tggccaatgg catgccaaaa
     1801 tcgaagccga tgacgccggt gtcgatctga tattgcgtcg ccggcgatgc cctggcgtcg
     1861 ctaccgtcca gataggcgtc gacaaagcga cgtccgatat cgatgtaggg cgacatatag
     1921 acaccgaagg tgccggaggc gccatatatc ggctggcgca gttcgctgtg caggctggtc
     1981 gactcgttgc cgagaatgag atcgctgcgc cactccagac cggtctgcgt catccacgga
     2041 tagcggtggc caatttgcag attgacgttg ccgtgaccgt cgaaagtgtt ggaaatgcca
     2101 agaccgaaca acaggtattg tggcccccag gatttttctt cgacatcaat ccgcagtaca
     2161 ttgcgcccgc ccttgctcaa catctcctgc gtcagcgcgt tgaagtcgcc attggtcgac
     2221 agttgcgaca actgctggtt gaggctatcg gcatcgtaga catcgccttc cttgatattc
     2281 agacggctgc gcacatagct cgcaggaata cggccggtat tgttgatctc gatggcatcg
     2341 atatggatgt ccctgggcag cgtcacactg tgatggcgcg atgtcagata ggcttgccat
     2401 tgctgcggcg ataacgacag ctcggccagg cgcccgctct gcgcctgagc cgcctcccag
     2461 ccagccttga taccgtcttt accgcgcgcg aaatcggcga acgacatgcc ggacaactca
     2521 ggttcaatga gaatatcgtg ttcccctagc agcgtttttt gcgccttgac attctgctgg
     2581 atcaggatgc cgatcatctg ttgcagcacg gcgggcggcg attgcagggt ggcgggatca
     2641 tgcagcggcg aagcgatatt gacggcgatg atgatgtccg cacccatgcc gcgcgcttcc
     2701 tgcaccggca ggttgctgac cagaccgcca tcgaccagta tacggccatc gatctgataa
     2761 ggcgcgaaca ggcccggcac cgccatgctg gcgcgcatgg cgcgcggcag ggaacctttg
     2821 tcgagaatca cctccgtacc ctgaccaaga tcggtggcga cggcgcggaa gggaatcggc
     2881 aacaggtcga aggatatatc cgcctgcaac tgtgccgtcc agttttgcag cagcgccagc
     2941 aggttgttgc cttgcaccag tccggtcgcc agcttcaact gaccatcgtc ataaccggcg
     3001 gcaatcgaga tcggatattg aaaatcatct tcgcgctgcg actgtggcag ctcggcgcgc
     3061 tcgctgcggt cgaaagcaat gtcgttgaga ttcatgtgca acagacggcg ctccagttcc
     3121 tgcgccgaga taccgctggc gtacaggccg ccgatcagcg cccccatgct ggttgcagcg
     3181 atgtaatcga ccggaacacg caattcttcc agatactgca acacccccag atgggcgtag
     3241 ccgcgcgcgc cgccgccgga gagtacgaga ccgatacgcg gacgcgatgc tgcatccgca
     3301 ccattgtcgg cggcatgggc gcaaggcagc agcaacccag cgagtaaagc agcccatgac
     3361 atgcagaacg caagacgaaa aaacatcagt gcagcgcccg gaatgtattg atacgcgtga
     3421 tactcatcct tgcctctttt tgccgtcttg cgccccgatg atggcgacgt agaatttcat
     3481 gaaaactggc gcaggtattg aagatgatat gcgattatag cggtcaacaa gacgtaatcc
     3541 gtatcgacga ggaaagaaga aagcttgttc agactgtctt gaccggtttg accttggccg
     3601 ccgcctgctt ggcgcggata cgcgcgtcat cgatgtcgtc ggcgacagcc agaactacgc
     3661 ccatgcggcg gcgtgcaaag gattccggtt tgccgaacag acggatgtcg gcgcccggga
     3721 tgcgcaaggc atcggccaca ccgtcgaaag cgatggcttt ggcgtcgtgc cggccataga
     3781 tcacagcgga ggcgcccggc gtgcgcaacg tgacattgac cggcaggccg agaatcgccc
     3841 tggcgtgcag ttcgaattcg ctctggatct gggtgatcat ggtcaccatg ccggtatcat
     3901 gcgggcgcgg gctgacttcg gagaaccaga ccatgtcacc cttgacgaac agttcgacgc
     3961 caaacaggcc gaggccgccg agatcgtccg tcaccttgcg cgcgatgtca cacgccttct
     4021 ccagcgcgac cggatgcatg ggatgcggct gccaggattc gacataatcg ccttgcacct
     4081 ggatgtggcc gatcggctcg cagaaatgcg tggcgatgct gccatcctgt tgcagtgctc
     4141 tgact
//
LOCUS       DASRDH010000179         6082 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1666531,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000179 DASRDH010000000
VERSION     DASRDH010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 6082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6082
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1666531"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /gene="lexA"
                     /locus_tag="VFA14_04115"
     CDS             complement(<1..527)
                     /gene="lexA"
                     /locus_tag="VFA14_04115"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HZS80596.1"
                     /translation="MLKLTARQEQILNLIKDAIENTGFPPTRAEIASALGFRSANAAE
                     EHLQALARKGAIEISPGTSRGIRLREIRPSLFGSRQMALPHPALIQLSLPLVGRVAAG
                     SPILAQEHIESESNYSIDPMLFSSKPDYLLRVRGMSMRDAGILDGDLLAVQKTREAKN
                     GQIVVARLGDEVTVKR"
     gene            753..1121
                     /gene="rpsF"
                     /locus_tag="VFA14_04120"
     CDS             753..1121
                     /gene="rpsF"
                     /locus_tag="VFA14_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008113362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HZS80597.1"
                     /translation="MRHYEIVFIVHPDQSEQVPAMIERYKGIVTARGGKIHRVEDWGR
                     RQLAYPIQKLAKAHYACLNIEIDGETLAEIETSFKFNDAVLRHLTVKMKKAETGPSPI
                     VKAVQKEDAVKGSRAEAPAA"
     gene            1156..1449
                     /gene="priB"
                     /locus_tag="VFA14_04125"
     CDS             1156..1449
                     /gene="priB"
                     /locus_tag="VFA14_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016833917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HZS80598.1"
                     /translation="MNQFQLVASIAERDVIRYTPAGIPVVTAKLQHQSEQLEAGIGRL
                     VEFEIVAFAAGEISGRFHQAGLGEIFRFIGFMARKNRRSKSLVFHIIDFESVV"
     gene            1484..1768
                     /gene="rpsR"
                     /locus_tag="VFA14_04130"
     CDS             1484..1768
                     /gene="rpsR"
                     /locus_tag="VFA14_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017776738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HZS80599.1"
                     /translation="MAFGKKFDKSKLKQKRQQQNPLFKRKKFCRFTAAGVEQVDYKDI
                     DTLKDFVQENGKIMPARLTGTKAHYQRQVDTAIKRARYLALLPYTDLHNA"
     gene            1795..2247
                     /gene="rplI"
                     /locus_tag="VFA14_04135"
     CDS             1795..2247
                     /gene="rplI"
                     /locus_tag="VFA14_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HZS80600.1"
                     /translation="MQVILLDKVVNLGGLGEVVRVKDGYARNFLIPRRMARRATASAI
                     AEFETKRAELAKAAAEKLAAAQAQGEKLGGLTVRISQKSGVDGRLFGSVTNFDIAEAL
                     TKQGFPVEKAQIRLPHGPLKIVGDHVVSVTLHTDVVVDVTIAVFGDHA"
     gene            2372..2875
                     /locus_tag="VFA14_04140"
     CDS             2372..2875
                     /locus_tag="VFA14_04140"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HZS80601.1"
                     /translation="MSPISNASGTLCIIVATDRQNGIGINDTLPWHLPEDLAHFKRAT
                     LGHPVIMGRKTFDSIGRALPGRRNIVVTRNRDWRYAGVETASSLQEAVALTVGADTFI
                     IGGARIYAESLPLATRLIVTEIAKSFDCDAFFPFIDPQLWQEVARESHRAATQEFDYA
                     FVTYERR"
     gene            2900..5167
                     /locus_tag="VFA14_04145"
     CDS             2900..5167
                     /locus_tag="VFA14_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/lysine/ornithine decarboxylase"
                     /protein_id="HZS80602.1"
                     /translation="MKFRFPIVIIDEDFRSENTSGLGIRALADAIQAEGMEVLGVTSY
                     GDLSQFAQQQSRASAFILSIDDEEFGAGSSEETDFALKSLRAFVSEIRHKNADIPIYL
                     YGETRTSRDIPNDILRELHGFIHMFEDTPEFVARHIIREAKSYLDSLAPPFFRALVHY
                     AQDGSYSWHCPGHSGGVAFLKSPIGQMFHQFFGENMLRADVCNAVEELGQLLDHTGPV
                     ANSERNAARIFNADHCYFVTNGTSTSNKMVWHSTVAPGDIVVVDRNCHKSILHSIIMT
                     GAIPVFLMPTRNHLGIIGPIPLEEFMMESIYKKIEANPFAREAVNKKPRILTITQSTY
                     DGVIYNVETLKQMLDGEIDTLHFDEAWLPHATFHDFYKSMHAIGKDRPRARKSMIFST
                     QSTHKLLAGLSQASQILVRESETTRLDQDAFNEAFLMHTSTSPQYSIIASCDVAAAMM
                     EAPGGTALVEESILEALDFRRAMKKIAQEFGQDWWFQVWGPNDFSADGIGAREDWVIK
                     AEDDWHGFGNLAPGFNMLDPIKATIVTPGLSLDGQFGQTGIPASIVTKYLAEHGVIVE
                     KCGLYSFFIMFTIGITKGRWNTLVTALQQFKDDYDKNQPIWRILPEFAAANQGRNVRY
                     ESLGLRDLCQQIHETYRIYDVARLTTEMYLSDMQPAMKPSDAFSKMAHREIDRVPLDE
                     LEGRVTSILLTPYPPGIPLLIPGERFNRTIVDYLKFARDFNEKFPGFETDVHGLVKRE
                     VDGRRDYFVDCVRQQ"
     gene            complement(5191..5301)
                     /locus_tag="VFA14_04150"
                     /pseudo
     CDS             complement(5191..5301)
                     /locus_tag="VFA14_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071012.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
     gene            5468..>6082
                     /locus_tag="VFA14_04155"
     CDS             5468..>6082
                     /locus_tag="VFA14_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014724247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase"
                     /protein_id="HZS80603.1"
                     /translation="MNPNDVRSKAFSMPLTSPAFPMGPYRFVGREFLIITYRTDPDRL
                     REVVPEPLKIAAPLVHYEFIRMADSTGFGDYTESGQVIPVEFEGQAGGYTLAMYLDDH
                     PPIAGGRELWGFPKKLASPMLRVDTDHLLGTLDYGKVRVATGTMGYKHRELDVAEQQK
                     RLEGSNFLLKIIPHVDGSARICELVQYPLLDVTLHGAWTGPAALD"
BASE COUNT         1349 a         1653 c         1695 g         1385 t
ORIGIN      
        1 cgcttgacgg tgacctcgtc gcccaggcgc gccaccacga tctgaccgtt cttggcctcg
       61 cgcgtctttt gcaccgccag caggtcgcca tcgaggatgc cggcatcgcg catcgacata
      121 ccacgcactc tcagcagata gtcgggtttg cttgaaaaca gcatgggatc gatgctgtaa
      181 ttgctttcac tttcaatgtg ttcctgcgcc aggatcggtg agccggcggc gacgcgccca
      241 accaggggga gtgagagctg tatcagcgcg ggatggggca gcgccatctg gcggctgccg
      301 aacagagagg gccggatttc gcgcaagcga atgccgcgtg aggtgcctgg cgaaatttca
      361 atagcgcctt tgcgcgccag ggcttgcaaa tgttcttcgg ccgcgttggc ggagcgaaag
      421 cccagtgcgc tggcgatttc tgcgcgtgtt ggtggaaaac cggtattttc aatggcatct
      481 ttgatcagat tgaggatctg ttcctggcga gcggtcagtt tcagcatgtc ggttttcagg
      541 gggaggggag ggcttattgt actggttgta tgaacagtct gtatttttat acagtcatca
      601 ggcagtgtca atgagcaggg cagtctgcaa gcaagggcct gaatgtattg agattttttg
      661 atttttgctg tactctcgcc agctttcctc ttcccacact cgtcttgacg ccggggtggg
      721 cgatttgcca atcagtccaa aaaggagttt tcatgcgtca ttatgaaatc gtatttatcg
      781 tccacccgga ccaaagtgag caggttccag cgatgatcga gcgttacaaa ggcatcgtta
      841 ctgcgcgtgg cggcaagatt catcgcgtcg aagattgggg tcgccgtcag ttggcgtacc
      901 cgatccagaa actggcgaaa gcccattatg cttgcctgaa tatcgaaatt gacggcgaaa
      961 cccttgctga aatcgaaacc agcttcaagt tcaacgatgc tgtgttgcgt catctgacag
     1021 tcaagatgaa gaaggccgaa actggtcctt cgccaatcgt gaaggctgtc caaaaggaag
     1081 atgcggtaaa gggcagtcgc gcagaggcgc cagcagcttg attgctgatg ccgcgataga
     1141 agagtgtcag ggagagtgaa ccagttccag cttgttgcca gcattgccga gcgtgacgtc
     1201 atacgttaca ctccggccgg cattccggta gtgaccgcga aattacagca ccagtcggaa
     1261 cagcttgaag caggtatcgg gcgattggtc gaatttgaaa tcgttgcatt tgccgcaggt
     1321 gagatttcag gcaggtttca tcaggctggt cttggcgaga tattcaggtt catcggcttc
     1381 atggcgcgta aaaatcgtcg cagcaaaagt ctcgtgtttc acatcattga ttttgaatcc
     1441 gtggtgtaga gccgcctagt ttttatagat caggagccgc aaaatggcat tcggtaaaaa
     1501 atttgataaa agtaagttga agcaaaagcg ccagcagcaa aatccgctgt tcaagcgcaa
     1561 aaaattctgc cgtttcactg cagccggtgt tgagcaggtc gattacaagg atatcgacac
     1621 cttgaaggat ttcgtgcagg aaaacggcaa gatcatgcct gcgcgtctga ccggcaccaa
     1681 ggcgcattat cagcgccagg tcgacacagc gatcaagcgt gcgcgctatc tcgccttgct
     1741 gccatacact gatttgcata atgcgtgatc gacgacgaga tacaggagaa aaatatgcaa
     1801 gtcattctat tggataaagt cgtcaatctc ggtggcctgg gcgaagtcgt ccgtgtcaag
     1861 gacggttatg cgcgtaactt cctgatcccg cgacgcatgg cgcgtcgcgc cacggcgagc
     1921 gctatcgctg aatttgaaac gaagcgcgcc gagctggcaa aggcagctgc agaaaagctg
     1981 gcggcagcgc aagcgcaagg cgaaaagctg ggtggcctga ctgtccggat ttcgcaaaaa
     2041 tccggtgtcg atggtcgcct gttcggttcc gtgaccaact ttgatattgc tgaagcgctg
     2101 acaaaacagg gctttccagt tgaaaaggct caaatccgtc tgccgcacgg cccactgaag
     2161 atcgttggcg atcatgtggt ttcggttact ctgcataccg atgtcgtggt tgatgtgact
     2221 attgctgtct ttggcgacca cgcgtagttt tttacgccat ttcgtttctt gcttgatatg
     2281 aaaaagtcgg gttcgtctgg cttttttatt gcgtgatcca tcctggactc gcggtgtacc
     2341 ggcttctggc aatcctgcac aaaagatcag catgtcacct atttccaacg cttccggtac
     2401 gctgtgcatc atcgtcgcta ctgacaggca gaacggtatc ggcatcaacg acaccttgcc
     2461 ttggcatctg ccggaagacc tcgcccattt caagcgtgcc acgttgggtc atccggtcat
     2521 catgggtcgc aagaccttcg attcaatcgg gagggcgctg ccgggtcgcc gtaatatcgt
     2581 tgttacccgc aaccgcgact ggcggtatgc cggcgtcgaa acggcttcat ctttacagga
     2641 agccgtcgca ttaacagtcg gcgcggatac tttcatcatc ggcggcgccc ggatttatgc
     2701 cgaatcgctg ccgctggcga cgcgcctgat tgtgactgaa atcgccaaaa gcttcgactg
     2761 cgacgctttt tttccattca tcgatcccca gctatggcaa gaggtggcac gggaaagcca
     2821 tcgcgccgca acccaggaat ttgactacgc atttgtgaca tatgaaagac gctgaaccga
     2881 ccgagaatct ggagggccta tgaaatttcg ttttcccatc gtcatcattg atgaagattt
     2941 tcgctctgaa aatacctctg gtctcggcat tcgtgcgctg gctgatgcca tccaggcaga
     3001 gggcatggaa gtattgggag tgaccagcta cggcgatctg tcgcaatttg cccagcagca
     3061 atcgcgcgcg tccgctttca tcctgtcgat cgacgatgaa gagttcggcg ccgggtcttc
     3121 tgaagaaacc gatttcgcgc tcaaatcgct acgcgccttc gtcagcgaga ttcgccacaa
     3181 gaacgccgac attccgattt acctgtatgg cgaaacccgt acctcgcgcg atattcctaa
     3241 cgacattctg cgcgagttgc acggcttcat tcacatgttt gaagatacgc cggagttcgt
     3301 ggcgcgtcac atcatccgcg aagccaaatc ttaccttgac agcctggcgc cgccgttctt
     3361 ccgcgcactg gtgcattacg cgcaggatgg ttcgtattcg tggcactgcc ccggccattc
     3421 gggcggtgtg gcattcctga aatcgccgat cggccagatg ttccatcagt tcttcggtga
     3481 aaacatgctg cgcgccgacg tctgcaatgc cgttgaagag ctcggccagc tactcgacca
     3541 taccggcccg gtcgccaatt cggaacgtaa tgccgcgcgt attttcaatg ccgaccattg
     3601 ctatttcgtc actaatggca cctcgacttc caacaagatg gtgtggcatt cgacggtggc
     3661 gcccggcgat atcgtggtgg tcgatcgcaa ttgccacaaa tccatcctgc attccatcat
     3721 catgactggc gcgattccgg tgttcctgat gccgacgcgc aaccatctcg gcatcatcgg
     3781 gccgattccg ttggaagagt tcatgatgga aagcatctat aagaaaatcg aagccaatcc
     3841 gttcgcgcgt gaagccgtga acaagaaacc gcgcatcttg accatcacgc aatcgaccta
     3901 cgatggcgtg atctacaatg ttgaaaccct taagcagatg ctcgacggtg aaatcgacac
     3961 cctgcacttc gacgaagctt ggctaccgca tgcaaccttt catgatttct acaaaagcat
     4021 gcacgccatc ggtaaggatc gtccgcgcgc caggaaatca atgatttttt cgacccaatc
     4081 gacccacaag ctgctggccg gcctgtcgca ggcgtcgcag attctggtgc gcgaatcgga
     4141 aaccacgcgg ctcgatcaag atgccttcaa cgaagctttc ctaatgcata cctcgacctc
     4201 gccgcaatac tcgatcatcg cttcgtgtga tgtcgccgcg gccatgatgg aggcgccggg
     4261 cggcactgcg ctggtggaag aaagcattct ggaagcgctg gattttcgcc gcgccatgaa
     4321 aaagatcgcc caggaatttg gtcaggactg gtggtttcag gtctgggggc cgaacgattt
     4381 ttctgctgac ggcatcggtg cgcgcgaaga ttgggtcatc aaggcggaag acgactggca
     4441 cggctttggt aatctggcgc cgggcttcaa catgctcgac ccgatcaaag ctactattgt
     4501 cacaccgggc ctgtcgctgg acggccagtt cggccagacc ggcattccgg cctcgatcgt
     4561 caccaagtat ctggcggaac atggcgtcat cgtcgaaaag tgtggcctgt actcgttttt
     4621 catcatgttt accatcggta tcaccaaggg ccgatggaac acgctagtca ccgccttgca
     4681 gcagttcaag gacgactacg acaagaacca gccgatctgg cgtatcctgc ctgagttcgc
     4741 agccgccaat caaggccgca acgtgcgtta cgaatctctg ggtctgcgcg acctgtgcca
     4801 gcagatccac gaaacctatc gcatctacga cgtcgcgcgc ctgacgacgg aaatgtatct
     4861 gtccgacatg cagccagcga tgaagccatc cgatgcgttc tcgaaaatgg cacaccgcga
     4921 aatcgaccgc gtgccgctgg atgagctgga aggccgcgtc acctcgatcc tgctgactcc
     4981 ttatccgcct ggcatcccgc tgctgattcc cggcgaacgc tttaacagga ccattgtcga
     5041 ttacctgaag ttcgcgcgcg atttcaacga gaaattcccc ggtttcgaaa ccgatgtgca
     5101 tggattggtc aagcgggaag tggatggacg gcgcgattat ttcgtggatt gcgtcagaca
     5161 gcagtagcct gtcggcgctc agcgaagaaa caccatgacg atggtcacca acgatgacgg
     5221 gtcaaacttc gtcagcgcca tcaccagcat cccgtaaaaa ccggctacgt accagccgat
     5281 caaagtcacg atcatcagat tgcgcgtgtt atgaactatg aaatagtcca taacacaagg
     5341 gatagtctga ccctgtaagc ttgctttgcg caccgtttca agaaattgcc atgcctgtct
     5401 gtatccccga ttctggcata gaatgggaaa ttcctgatcc acggcacaat gcaaggaggg
     5461 ggtgcgcatg aatccaaacg atgtacggtc gaaggcattt tcgatgccgc tcactagccc
     5521 tgcattcccg atgggaccct accgtttcgt cggccgcgaa ttcctgatca ttacctaccg
     5581 caccgatccc gaccggctgc gcgaggttgt gcccgagccg ctgaagatcg ctgcgccgct
     5641 tgtgcattat gaattcatcc ggatggcgga ttcgacgggc tttggcgact acaccgagag
     5701 cggtcaggtg atcccggtcg aattcgaagg tcaggcgggc ggctatacgt tagcgatgta
     5761 tctcgatgat catccgccca tcgctggcgg ccgcgaactg tggggtttcc cgaagaagct
     5821 cgcgtcgccg atgctgcgcg tggataccga ccatctgctc ggcacgcttg attacggcaa
     5881 ggtgcgagtt gcgactggca cgatgggata caagcaccgc gaacttgacg ttgctgagca
     5941 gcagaagcgc cttgagggat ccaactttct gctgaagatc attccgcacg tggacggcag
     6001 cgcgcgcatc tgcgagctgg tgcaatatcc gcttctcgac gtgacgctgc atggcgcatg
     6061 gacggggccg gcggcgctcg at
//
LOCUS       DASRDH010000180         3018 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1511564,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000180 DASRDH010000000
VERSION     DASRDH010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3018
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1511564"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..343
                     /locus_tag="VFA14_04160"
                     /pseudo
     CDS             2..343
                     /locus_tag="VFA14_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008330879.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
     gene            527..1942
                     /locus_tag="VFA14_04165"
     CDS             527..1942
                     /locus_tag="VFA14_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HZS80604.1"
                     /translation="MPASSSFLQACRAVIGTAYVLTDATDTAAYLTDQRKRYTGRALA
                     VLKPGSSEEVAAIVKLCHQQRIPIVPQGGNTGLVLGSVPDSSGRAVLLSLTRLNRFRT
                     IDPVNNTMTVEAGCILYDIQQAAAAAQRLFPLSLAAESSCTIGGNLSTNAGGTSVLRY
                     GNTRELCLGLEVVTAQGDIMSNLRGLRKDNTGYDLRDLFIGAEGTLGIITAAVIKLFP
                     QPQAQVTALAALRTPNHALRLLNLAQSRCGAALTGFELMSDFCLQLVTRHFPPQRVPF
                     DKPHAQYVLLELSDSESAMHATAMLEAVISTALERDLIDDAVIATSIAQSKLLWQLRE
                     HISMAQAQEGHNIKHDISVPISRIDEFIRVTDALLQEISPGCRIVSFGHLGDGNLHYN
                     VSPPPGVKNTDFIMQQPKINRMVHDSVDMFGGSISAEHGLGALKREEIRRYKSPVELQ
                     LMRAIKQALDPHALMNPGKVI"
     gene            complement(1995..>3018)
                     /locus_tag="VFA14_04170"
     CDS             complement(1995..>3018)
                     /locus_tag="VFA14_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZS80605.1"
                     /translation="ADYGVSAYFGLSTFTSGIYKAWMVMDDRIAAAQYASLLLLFVAV
                     LLWLERREQQRMRFSGARSHRSDGNEGAESRLPSLRGAQAALAWLVCAMPIVLGFVLP
                     VLILLQLMWRESEMLLTIDLMRYAGWSWNSFRFGSMAAGIAVVLALALCFAPRAGGLP
                     RFHHTFLRVVARMASMGYAIPGSVIAIGILLPVAWLQAAFPGSMAGVWLTATSLGVIY
                     AYLVRFTAVAVQSVEAGYTRIPLHVDEAARIMGASRRCIVQRLHVPLLWRSLVTAGLM
                     VFVDVVKELPATLLLRPFDTDTLAVIAHNLARDERLGEAALPALSIVAVGLVPVWLVT
                     RALDKR"
BASE COUNT          690 a          989 c          812 g          527 t
ORIGIN      
        1 gatcgcactg atcttcctgt gcgtgacctg ggaactgcta ctggcaccgc tacggccagg
       61 tgggtcatgg atggtgttca aggtcattcc cctgctgttt ccgctacgtg gcgtactcaa
      121 gcgcgatatc tacaccatgc aatggtcttc gatgctgatc ctgctgtatt tcgcggaagg
      181 tatcgtgcgc gccaccagcg atcatgccgc tacggcgatc atgctgggct ggatcgaaat
      241 cgcattgagc tgcctctatt tcctgtgcac cctttgctat ctgcacccct acaaaaaagc
      301 agccggaacg atcgcccgcc aggcgatccg gaaagcccca gaacgtgttc acgatcttgt
      361 cgggagcgga cctatcgtcg tttcaggatg ggatgcaagg cgcaaaacgc agacgggcca
      421 gtggcccgat gaagctttgc aacgccgcag atcacctgaa agggcggtag gacgaccccg
      481 gaggagatcg tgaacacgtt ctcatgataa tcttcccaag ctttccatgc ctgcttcatc
      541 ctctttcctg caagcctgcc gcgccgtcat cggaaccgct tacgtcctca cggacgccac
      601 cgacaccgct gcctatctga ccgaccagcg caaacgctac accggccggg cgttggccgt
      661 gctcaaaccc ggcagcagtg aggaagtcgc cgccatcgtc aaactgtgcc accagcagcg
      721 gatacctatc gtgccgcaag gcggcaacac cgggctggtg ctgggcagcg tgccggacag
      781 cagcggccgc gcggtgctgc tgtcactgac acggctcaat cgcttccgca ccatcgatcc
      841 ggtcaacaac acgatgacag tcgaagccgg ctgcatccta tacgacattc agcaggccgc
      901 cgccgccgcg cagcgcctgt tcccactgtc gctggcagcc gaaagcagtt gcaccatcgg
      961 cggcaacctg tcgaccaatg ccggcggcac cagcgtcctg cgctacggca atacgcgtga
     1021 gctgtgtttg ggcctggaag tcgtcaccgc gcagggcgac atcatgagca acctgcgcgg
     1081 actgcgcaag gacaacaccg gctacgacct gcgtgatctg ttcatcggcg ccgaaggcac
     1141 actaggcatc atcaccgccg cggtcatcaa actgttcccg caaccgcagg cgcaagtcac
     1201 cgcattagca gcgctgcgca cgccgaatca cgcgctacgc ttgctgaatc tggcacagtc
     1261 gcgctgcggc gcagcgctga ccggcttcga gctgatgtcg gatttttgcc tgcaactggt
     1321 gaccaggcat ttcccgccgc agcgcgtgcc attcgataaa ccgcacgcgc agtatgtttt
     1381 actggaactc tccgacagcg aatccgccat gcacgctacc gccatgctgg aagccgtgat
     1441 cagcacggca ctggaacggg atctgatcga cgacgccgtg atcgccacct cgatcgcgca
     1501 atcgaagttg ttatggcaac tgcgcgaaca tatttcgatg gcgcaagcgc aggaaggcca
     1561 taacatcaag cacgacatct cagtgcccat ttcacgcatc gacgaattca tccgtgtcac
     1621 cgacgcgctg ttgcaggaaa tttcaccagg ctgccgcatc gtgagctttg gtcatctcgg
     1681 cgatggcaac ctgcactaca atgtatcgcc gccgcctggc gtcaaaaata ctgacttcat
     1741 catgcaacag ccaaagatca atcgcatggt gcatgacagt gtcgatatgt tcggtggttc
     1801 catctcggcc gagcatggtc tgggcgcgct caagcgcgaa gaaatccggc gttataaatc
     1861 accggtggag ctgcaactga tgcgcgcgat caaacaggcg ctggatccac atgccttgat
     1921 gaatccggga aaagtcatct gatcccctgc ccagataacg atagatcatc atcgtatcgg
     1981 cgtatggttt tcctttatcg tttatccagc gcgcgcgtta ccagccatac cggcaccagc
     2041 ccgaccgcga cgatactcag cgccggcagg gccgcttcgc cgagacgttc gtcacgcgcc
     2101 agattgtgag caatcacagc cagcgtatcg gtatcgaaag gccgtagcaa cagcgtcgca
     2161 ggcaattctt tcaccacatc gacgaacacc atcaggccgg cagtcaccag cgaacgccac
     2221 agcaacggca catgcagtcg ttgcacgatg cagcggcgcg atgcccccat gatgcgcgcc
     2281 gcttcatcga catgcaacgg aatgcgggta taaccggctt ccaccgactg caccgccacg
     2341 gccgtaaaac gcaccagata cgcataaatc acaccgagcg acgtcgccgt caaccagaca
     2401 cccgccatgc tgcccgggaa cgccgcctgc aaccaggcga ccggcagcag aatgccgatc
     2461 gcgatcaccg aaccaggaat cgcgtaaccc atggatgcca tgcgcgcaac cacgcgcaga
     2521 aaggtatgat gaaaacgcgg caaaccaccg gcgcgcggcg cgaaacacaa ggccagcgcc
     2581 agtacaacgg caataccggc agccatgctg ccgaagcgaa agctgttcca actccagccg
     2641 gcataacgca tcagatcgat cgtcagcaac atctcgcttt cgcgccacat cagttgcagc
     2701 aaaatcagca ccggcagcac gaaacccagc acgatgggca tagcgcatac cagccaggcc
     2761 agcgccgctt gcgcgccccg caatgacggc aaacgcgatt cggcaccctc attgccatca
     2821 ctacgatggc tgcgcgcgcc ggaaaaacgc atgcgttgct gctcacgacg ctccagccat
     2881 aacagcacgg cgacgaacag caacagcagc gacgcatact gcgcagcagc gatgcggtca
     2941 tccatcacca tccaggcctt gtagatgccg ctagtgaatg tactcaaacc gaaataagcg
     3001 ctcacgccat agtcggcc
//
LOCUS       DASRDH010000181         2433 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_134040,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000181 DASRDH010000000
VERSION     DASRDH010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134040"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..897)
                     /locus_tag="VFA14_04175"
     CDS             complement(<1..897)
                     /locus_tag="VFA14_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZS80606.1"
                     /translation="MIEIQALTQRFRSGNSTVEAVRNVDLSIRPGEIFGIIGRSGAGK
                     STLVRTINLLSRPSSGRVILDGEDMTTLPAAALRAARRRIGMIFQHFNLLSSRTVYDN
                     IALPLELAGLCKTGIAGKVEPLLELVGLTDLQDRYPAQISGGQKQRVGIARALANDPK
                     VLLSDEATSALDPETTRAILELLRRINRELGLTIVLITHQMEVIKMICDRVAVMEAGQ
                     VIEQGDVLDVFRAPRHAVTRALIGDVIAHELPKGVLERLRARLAGTAAETGPASNAGH
                     LFRFAFTGSSVDQPHLSEAVRRF"
     gene            complement(1165..>2433)
                     /gene="groL"
                     /locus_tag="VFA14_04180"
     CDS             complement(1165..>2433)
                     /gene="groL"
                     /locus_tag="VFA14_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008115203.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HZS80607.1"
                     /translation="AELKKISKPCTTSKEIAQVGAISANADPEIGKTIADAMDKVGKE
                     GVITVEDGKSLDNELDIVEGMQFDRGYLSPYFINNQEKQVVALENPYVLLFDKKVSNI
                     RDLLPVLEQVAKAGRPLLIIAEDVEGEALATLVVNNIRGILKTAAVKAPGFGDRRKAM
                     LEDIAILTNGQVIAEEVGLTLEKATLAELGQAKRIEIGKENTTIIDGNGEATAIEARV
                     KQIRTLIEEATSDYDREKLQERVAKLAGGVALIKVGAATEVEMKEKKARVEDALHATR
                     AAVEEGVVPGGGVALLRARASIKDLKGDNADQDAGIKIVLRAVEEPLRQIVCNAGDEP
                     SVVVNAVLAGTGNYGYNAANGTYGDLVELGVLDPAKVTRSALQNAASVAGLILTTDAL
                     VAELPEDKAAGGMPGGMGGMGGMGGMDGMM"
BASE COUNT          415 a          742 c          728 g          548 t
ORIGIN      
        1 gaagcggcgc actgcttccg atagatgcgg ctgatccacg ctcgagccgg tgaaggcgaa
       61 gcggaacagg tggccggcgt tgctggccgg gccggtttct gccgccgtcc cggccaggcg
      121 cgcgcgcagg cgctccagca cgcccttggg caattcatgt gcgatcacat cgccgatcag
      181 cgcgcgtgtg acggcatgtc tgggcgcgcg gaatacgtcc agtacatcgc cctgttcgat
      241 cacctgaccg gcttccatca cggcgacgcg gtcgcagatc atcttgatga cttccatctg
      301 gtgcgtgatg agtacaatcg tcaggcccag ttcccggttg atcctacgca gcagttcaag
      361 gattgcgcgc gtggtttccg gatcgagcgc ggaagtggct tcatccgaca acaacacttt
      421 gggatcgttg gccagggcgc gggcgatgcc gacgcgctgt ttctggccgc cggagatctg
      481 cgccggataa cggtcttgca agtctgtcag accaaccagt tccagcaagg gttcgacctt
      541 gccggcgatc ccggttttgc agagaccggc cagttccagc ggcagggcaa tattgtcgta
      601 cacggtacgc gaagacaaca gattgaagtg ctggaaaatc atgccaatcc ggcgccgcgc
      661 cgcgcgcagg gcagcggccg gtaacgtggt catatcctcg ccgtcgagaa tgacgcggcc
      721 ggagcttggg cgactcaaca gattgatggt acgcaccagc gtgcttttgc cggcgccgct
      781 gcggccgatg atgccgaaaa tttctcccgg gcggatagac agatccacgt tgcgtaccgc
      841 ctcgaccgtg ctgttgccgc tcctgaagcg ttgggtcagc gcctggattt cgatcatttt
      901 gctttctgtt gcgatgaaaa aagcagcagg ccggtcttgt gaaccgcctg ctgcctatat
      961 ttttttatag agtcgtaatt ttatgaggat ttcgtgaaac aggcgagcaa aacggaagcg
     1021 cgactccaat gcaaaacgcc cgatgcactg catcgggcgt tacgacggct actgtcgagc
     1081 cgttccgggc tggacagcgt ggctgggtgg aaagcctatg ctttccggct tgcctgtctt
     1141 ctggaaaagg caggcagcgc cggattacat catgccgtcc atgccaccca taccgcccat
     1201 gccacccata ccgcctggca tgccgccagc ggccttgtct tccggcaatt cagcaaccag
     1261 tgcgtcggtc gtcaggatca gaccggcaac cgatgcggca ttctgcaatg ccgagcgtgt
     1321 caccttggcc ggatccagta cacccagttc gaccaggtcg ccgtaagtgc cgttggcagc
     1381 gttgtagcca tagttgccgg tgccggccag cacggcattg accaccaccg atggctcgtc
     1441 gcctgcgttg cagacgattt gacgcagcgg ttcttcgaca gcgcgcagaa cgatcttgat
     1501 gccggcgtcc tgatcggcat tgtcgccctt gaggtccttg atactggcgc gcgcgcgcag
     1561 cagggcaacg ccgccgcctg gcacgacgcc ttcttcaacc gcggcgcgag tagcgtgcag
     1621 cgcgtcttct acgcgtgctt tcttttcctt catttcgact tcggttgccg cgccgacctt
     1681 gatcagtgcc acaccgcctg ccagtttggc aacgcgctct tgcagctttt cgcggtcgta
     1741 gtcggaagtt gcttcttcga tcagtgtgcg gatctgcttg acgcgcgctt cgatcgcggt
     1801 ggcttcgccg ttaccgtcga tgatggtggt gttttccttg ccgatttcga tacgcttggc
     1861 ttgacccagt tcagccagtg tggctttttc cagggtcagg ccgacttctt cagcgatcac
     1921 ctggccgttg gtcaggatag cgatgtcttc cagcattgcc ttgcgccggt cgccaaagcc
     1981 gggcgccttg acggctgcgg tcttcaggat gccgcggatg ttattcacga ccagtgtcgc
     2041 cagcgcttcg ccctcgacgt cttccgcgat gatcagcagt ggccggccgg ccttggcgac
     2101 ctgttccaga accggcaaca ggtcacggat gttcgatacc ttcttgtcga acagcagaac
     2161 gtatgggttt tccagagcga cgacctgctt ctcctggtta ttgatgaagt agggagatag
     2221 gtaaccgcgg tcgaactgca tgccttcgac gatgtccagt tcattgtcca gcgacttgcc
     2281 gtcttccacg gtgatgacgc cttccttgcc gaccttgtcc atcgcgtcgg cgatcgtctt
     2341 gccgatctcc ggatccgcgt tggccgagat cgcgccgacc tgcgcgattt ccttgctcgt
     2401 cgtgcacggc ttgctgatct tcttgagctc cgc
//
LOCUS       DASRDH010000182         4320 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_474776,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000182 DASRDH010000000
VERSION     DASRDH010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 4320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4320
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474776"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="VFA14_04185"
     CDS             complement(<1..406)
                     /locus_tag="VFA14_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 associated
                     domain-containing protein"
                     /protein_id="HZS80608.1"
                     /translation="MASNNVAQFATELKMPADLLLTQLRSAGVEKNSGSDPLSKEDKE
                     RLLEHLRRAHGAAPEGEKKKITLIRKETTEIKQADSTGKSRTIQIEVRKKRTFVKRDE
                     SVAEDFTSTAPVELVLDAEEVKRRAEEARRQAE"
     gene            complement(457..2019)
                     /gene="nusA"
                     /locus_tag="VFA14_04190"
     CDS             complement(457..2019)
                     /gene="nusA"
                     /locus_tag="VFA14_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HZS80609.1"
                     /translation="MSREVLLLVDALAREKNVDKDVVFGALEHALEQATRKRYEGEVD
                     IRVSIDRESGEFESFRRWHVVPDEAGLQLPDQEVLHFEAKEQIADIAVDEYIEEPIES
                     VEFGRRFAQDTKQVVLQRIRDAEREQILVDFLERGDSLVTGIIKRMERGDVIVESGKI
                     EARLPRDQMIPKENLRIGDRVRAYILRIDRNARGPQVILSRTAPEFIMKLFELEVPEI
                     EQGSLEIKSAARDPGMRAKIAVYTSDKRIDPIGTCVGMRGSRVQAVTGELGGERVDIV
                     LWSDDPAQFVIGALAPANVTSIVVDEEKHAMDVVVDEENLAIAIGRGGQNVRLAAQLT
                     NWQINIMTAEESADKSAQETATIRTLFMEKLDVDQEVADILVEEGFASLEEIAYVPIN
                     EMLEIESFDEDTVNELRNRARDALVTEAIASEEGLEGMDEALIDLEGMDRIVASKLGL
                     AGIKNVPDFAALAYDEFGAILALSVDRARQLIANEFEDVTDDEMKLIDAKYDERAKAL
                     QAKAWSTTEATQ"
     gene            complement(2016..2504)
                     /gene="rimP"
                     /locus_tag="VFA14_04195"
     CDS             complement(2016..2504)
                     /gene="rimP"
                     /locus_tag="VFA14_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041322554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HZS80610.1"
                     /translation="MQLSGLIEITLSGMGYELVDFERAARGLLRVFIDFPSENADRGS
                     ITVEDCEKVTHQLLRVLTVENVDYGRLEVSSPGLDRPLKKLSDYVRFAGQEAIVKLRM
                     PMPGAANRKSFQGILRVPEGENLKLEFETNDESVAVLDFTLADVDKARLVPKVDFRSR
                     KA"
     gene            complement(2768..4288)
                     /locus_tag="VFA14_04200"
     CDS             complement(2768..4288)
                     /locus_tag="VFA14_04200"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HZS80611.1"
                     /translation="MPKIIVTIGEEVTAAPVLLVARADNTRTRPVRYGVRSPRTLRNN
                     RVAQRAAETGETTQAHSAVDIAAVASGAPVSVGGRQAVAELRREGRSQDRGRQQKGKK
                     PFSKGGKPKAPQLGLRAHDVGDDIFSFVTSDAYDKEDGARGGARNGHQLRGKNGRRDL
                     TSDDDAPKLHKVLADSGLGSRRDMEELIVAGRVSVNGEPAHIGQRILPTDQVRINGKL
                     LQRKVSKKPPRVLVYHKPAGEIVSHNDPDGRPSVFDRLPNMKAAKWLAVGRLDFNTEG
                     LLLFTTSGDLANRLMHPRYNVEREYAVRTLGELEQGMRQKLLHGVELEDGMAQFSRIA
                     DGGGEGVNKWYRVTIGEGRNREVRRMFEAIGLTVSRLIRTRYGMMTLPQGLRRGRWEE
                     LEESAVRSLLAVCGLKKLDGQGALADKGRQDRSNGNGQPRGNANSVQPKDLRRESWQG
                     QGGGRSQQNRSRQPDPLQTALGFPDAGQRRQHRPQRGGMMGGQGMDAGGLPRRRRG"
BASE COUNT          932 a         1361 c         1046 g          981 t
ORIGIN      
        1 gctcagcctg gcggcgcgct tcttcggcac ggcgcttcac ttcctcagca tccagtacca
       61 gctcaacagg ggcagtggat gtgaaatctt ctgccacaga ctcatcacgc ttgacaaaag
      121 tacgcttctt gcgcacctct atctgaatcg tgcgcgactt cccggtggaa tcagcctgct
      181 tgatttcggt tgtttccttg cgaattaacg tgattttctt tttttcgcct tccggagcag
      241 cgccatgcgc acgacgcaga tgctccagaa gacgctcttt gtcttccttc gacaaaggat
      301 cggacccgga atttttttca actccggcag accgcagttg ggtcagtagc aaatccgccg
      361 gcattttcag ctcggtggca aactgggcta cattgttact tgccattcag tcctcttttc
      421 tatgtggctc agcagatgat gcaggtacgt gaaagcttat tgcgtggctt ccgtcgtact
      481 ccatgctttg gcttgcaatg ccttggcacg ctcgtcgtat ttggcatcga tgagcttcat
      541 ttcatcatcg gtcacatctt caaattcatt tgcaatcagt tgacgcgcac ggtcaactga
      601 caacgccagg atggcgccga attcgtcgta ggccagcgcc gcgaaatcag gcacgttctt
      661 gatgccagcc agaccgagct tgctggcgac gatgcgatcc attccctcga gatcgatcag
      721 cgcttcgtcc ataccctcca gaccttcttc cgacgcaatc gcctcggtca ccaaggcatc
      781 gcgagcgcga tttcgcaact cgttgacagt atcttcgtcg aaggattcga tctctaacat
      841 ttcgttgatc ggcacgtagg cgatttcttc caggctggcg aaaccttctt ccaccaggat
      901 gtcggcgact tcctgatcga cgtccagttt ctccatgaac agagtacgaa tcgtggcagt
      961 ttcctgtgct gacttgtcag ccgattcttc cgccgtcatg atgttgatct gccagttggt
     1021 caactgggca gccagacgca cgttctgtcc gccgcgaccg atggcgatgg ccagattttc
     1081 ttcgtcgaca acgacatcca tggcatgttt ttcttcatcc acgacgatgg aagtcacatt
     1141 ggcaggcgcc agcgcaccga taacgaattg cgccgggtcg tctgaccaca agacaatgtc
     1201 gacgcgctct ccgccaagct caccagtgac agcctgcacg cgcgaaccgc gcatgccgac
     1261 acaggtgcca atcggatcaa tgcgtttgtc gctagtatag accgcgatct tggcgcgcat
     1321 gcctgggtcg cgcgcggccg atttgatttc cagcgagcct tgttcaattt ccggcacttc
     1381 cagctcaaac agcttcatga taaattcagg cgcagtacgc gacagaatca cttgcggacc
     1441 gcgcgcattg cggtcgatac gcaggatgta agcgcggacg cgatcaccga tacgcaaatt
     1501 ttcttttgga atcatctgat cgcgcggcag acgagcctca atcttgccgg attcgacaat
     1561 aacatcaccg cgttccatgc gcttgatgat gccggtgacc agcgaatcgc cgcgttcaag
     1621 gaaatcaacc agaatctgtt cacgctcggc atcgcgaatg cgctgcagga cgacctgttt
     1681 agtgtcttgc gcaaaacggc gaccgaattc gaccgactca atcggctctt cgatgtattc
     1741 atcgacggcg atgtctgcaa tctgctcttt ggcttcgaag tgcaacactt cctggtcggg
     1801 caattgcaaa ccggcctcgt cgggcaccac atgccagcga cggaaagatt caaattcgcc
     1861 gctttcacga tcgatcgata cgcggatgtc gacttctccc tcgtaacgtt tcctggtagc
     1921 ctgctcaagc gcatgctcca gcgctccgaa gacgacatcc ttatcgacat tcttttcgcg
     1981 cgccagcgcg tcgaccaata ataaaacttc gcgactcatg ctttgcgact cctaaaatcc
     2041 actttcggca ccagacgtgc cttatccaca tcggcgagtg tgaaatccag cactgcaacc
     2101 gactcatcgt ttgtttcaaa ttccagtttc agattctcac cttcagggac gcgcaggatg
     2161 ccctgaaacg atttacgatt ggctgcgccc ggcatcggca tgcgcagctt gacaatcgcc
     2221 tcctgaccgg caaagcgaac gtaatcgctc agcttcttca gtgggcgatc cagccccggg
     2281 gacgaaactt ccaaccgccc gtagtcgacg ttttcaactg tcaacacacg caacaactga
     2341 tgcgtgacct tttcgcaatc ctcgaccgta atcgaccctc tgtcagcatt ttcagaagga
     2401 aaatcgataa atacacgcag cagaccgcgc gccgcccttt cgaagtcgac caactcgtac
     2461 cccattcccg acagggtaat ttcaatcaag cctgacaatt gcaaaaaatc tctccgcgct
     2521 gcgcgtctgc ggatacagac acctcaattt gttcagcaaa aaaaaatggg catctgccca
     2581 tcttttttat cgttcattca gcgtttatca acgacaaaca gatttgtcat accttgctcg
     2641 ccgcgatatt ttctcccgtt aacttatgta tcataaccga taccgccacg tccggcaatc
     2701 caggattgcc ggatacctat ctgaaagccg accatcccct cgaagaaaca gtccaacaat
     2761 ccatcattta cccgcgccgg cgacgcggta aaccgccagc atccataccc tgccccccca
     2821 tcataccgcc gcgctgcgga cggtgctgac gacgctggcc cgcatctgga aaacccagtg
     2881 ccgtctgcaa aggatccggc tgacgactcc tgttctgctg cgagcgaccg ccaccctgcc
     2941 cctgccatga ctcacgacgc aaatccttgg gctgcaccga attagcattg ccgcgcggtt
     3001 gaccattgcc gtttgagcgg tcttggcgcc ccttgtccgc cagcgcaccc tgcccatcca
     3061 attttttcag accacagaca gccagcaaac tgcgcacggc gctctcttcc agctcttccc
     3121 agcggccgcg cctgagtcct tgcggcaagg tcatcatgcc gtagcgggta cgaatcaggc
     3181 gcgatacagt caggccaata gcttcgaaca tgcggcgaac ctcacgattg cgaccctcac
     3241 ctatcgtcac acgataccat ttattgacgc cttcgccgcc gccatcggcg attctggaaa
     3301 actgcgccat gccgtcctcc agctcgaccc catgcaacag cttctggcgc atgccttgct
     3361 ccagctcgcc cagcgtacgc acggcgtatt cgcgctcgac gttgtaacgt ggatgcatca
     3421 agcggttagc cagatcgccg gaagtggtaa acaacaacaa accttcggta ttgaagtcca
     3481 gacgaccaac ggccagccat ttggcagcct tcatattcgg caggcgatca aatactgatg
     3541 gacggccatc cgggtcgtta tggctgacga tttcgccagc cggcttgtga tacaccagca
     3601 cacgcggcgg cttcttgctg actttgcgtt gcagcagttt gccattgata cgtacctgat
     3661 cagtcggcaa aatgcgctga ccgatatgcg ccggttcgcc gttgaccgac acacggccag
     3721 ccacgatcaa ttcttccatg tcgcgacgcg aacccaggcc ggaatcggcc agaaccttgt
     3781 gcagcttcgg cgcatcgtcg tccgacgtca aatcgcgacg accattcttg ccgcgtaatt
     3841 gatggccgtt ccgggcacct cccctggcac cgtcctcctt atcataggca tcggaagtca
     3901 caaaagagaa aatatcgtca ccgacatcat gcgcgcgcaa acccagttgc ggcgccttgg
     3961 gcttaccacc cttgctgaaa ggttttttgc ccttttgctg gcgcccacga tcctgactgc
     4021 gaccttcacg gcgcaattcc gccacagcct ggcggccgcc cacggaaacc ggcgccccgg
     4081 acgcaacagc tgcgatatcg acagccgaat gcgcctgcgt ggtctcaccg gtttcggcag
     4141 cacgctgggc aacacgatta ttgcgcaagg tacgtgggct acgcacacca taacgaaccg
     4201 gcctggtccg cgtattgtct gcacgtgcga ccagcaggac cggcgcagcg gtaacttctt
     4261 cgccaatggt cacgataatt ttcggcatct ccactgcaac agtagtcgga gcaatcactg
//
LOCUS       DASRDH010000183         3394 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_123148,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000183 DASRDH010000000
VERSION     DASRDH010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 3394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3394
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123148"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1500
                     /locus_tag="VFA14_04205"
     CDS             <1..1500
                     /locus_tag="VFA14_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017454120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HZS80612.1"
                     /translation="QGLPRLVATPCIEMLNAASLSFALCPLLTDGAIEALMTAGSDAQ
                     KNIYLANLISGRWTGTMNLTEPQAGSDLALICTRAVPQDDGSYRLSGSKIFITYGEHD
                     MAENIVHLVLARTPDAPEGVKGISLFVVPKFLVEPDGSLGERNDVHCMSIEHKLGIKA
                     SPTAVLRFGDHGGAIGMLVGEEHRGLEYMFIMMNIARFAVGIQGIGVAERACQKAVAY
                     AKERVQSRDLAGSAGAATIIHQPDVRRMLMSMRAQVEAARALAYVAAAASDVGHHHPD
                     EATRRANQACYEYLVPIVKGWSTELSIDVASTGVQVHGGMGFIEETGVAQYYRDARIL
                     SIYEGTTAIQANDLVGRKTVRDGGAVARGIIAQIRKTEAALGAVSGDHGDDLQAIGKR
                     LVDGSRALEEVVDYVVTGIRSDIKAVFSGSVPYLKLAGVVLGGWQMARAALVAVGRLR
                     AGEGDAQFYEAKIATARFYADHVLTQASSLRTSIVEGSAGVLALTEDQF"
     gene            complement(1518..1934)
                     /locus_tag="VFA14_04210"
     CDS             complement(1518..1934)
                     /locus_tag="VFA14_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007879927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS80613.1"
                     /translation="MIITHKNKTLTAFLAAISGGIGMHRFYLYGRKDLFGWLHAASLP
                     VSALLIALFSHIQPLFAGMLFVLSVLCAFLETLCIGLTSDEQWDARHNPASGRKSGSH
                     WILALILILALGIGAMAVIALLARSFDLLFTGGAYG"
     gene            complement(1945..2322)
                     /locus_tag="VFA14_04215"
     CDS             complement(1945..2322)
                     /locus_tag="VFA14_04215"
                     /inference="COORDINATES: protein motif:HMM:NF023401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS80614.1"
                     /translation="MDIRLPDRNQGAGRPTQRRAGRHRQADCRWLESIGDSQPKQRLE
                     RIFQPTRVGKLPPQLTALMRTDINMQELIVKTVLNQNHEHVYHAAMVDPHTAAVLDLE
                     QIRAMVDGLCCARRLPAHLGLHR"
     gene            complement(2220..3113)
                     /gene="bla"
                     /locus_tag="VFA14_04220"
     CDS             complement(2220..3113)
                     /gene="bla"
                     /locus_tag="VFA14_04220"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007880497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase"
                     /protein_id="HZS80615.1"
                     /translation="MTDRRHFLAMTATALSACVTGVWAAEEKKSGESSSVISCLGKLE
                     ASVDGRLGVEILDTGSGRHWGYRADERFPMCSTFKFLAASAVLQRVDAGAEQLDRRIV
                     YDADMLVPYSPTTGKHVGGDGLSLAQICEAAMTLSDNTAANLILRNLGGLTPLNDFIK
                     SLGDDLTRVDRFEPELNTALPGDARDTTTPAAMTGNLQKILLGNALSAASRQQITAWM
                     AANKTGDRRVRAGMPPGWKVGDKTGSGAYGTTNTIAIIWPPQGKPLLTSVYLTETRAP
                     EDQRNAVLADIGRLIVDGLSR"
     gene            3251..>3394
                     /locus_tag="VFA14_04225"
     CDS             3251..>3394
                     /locus_tag="VFA14_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007880490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003839 - gamma-glutamylcyclotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061928 - glutathione specific
                     gamma-glutamylcyclotransferase activity [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HZS80616.1"
                     /translation="MPDDTIACNKAMTRFDQHDSVWLFGYGSLIFKADFSFIERRPAH
                     IVGW"
BASE COUNT          678 a          947 c         1049 g          720 t
ORIGIN      
        1 cagggtttgc ccaggttggt ggcgacgcct tgcatcgaaa tgctcaatgc cgccagcctg
       61 tcgtttgcct tatgtccgct gctcacggat ggcgccatcg aagcgctgat gactgccggc
      121 agcgatgcgc agaaaaatat ctatctggcg aatctcatct ccggtagatg gaccggcacg
      181 atgaatttga ccgagccaca ggccgggtcc gacctggcgt tgatatgtac gcgcgccgta
      241 ccgcaggatg atggtagcta cagattgtcg ggcagcaaga ttttcatcac ttatggcgaa
      301 cacgacatgg ctgagaatat cgtccatctg gtgctggcgc gcacgccgga cgcaccggag
      361 ggtgtgaaag gcatttcgct gttcgtcgtg ccgaagtttc tggtcgagcc ggacggttcg
      421 cttggtgagc gcaacgatgt gcattgcatg tctatcgaac acaagctcgg catcaaggcc
      481 agtccgactg cggtgttgcg gtttggcgat cacggcggcg ccatcggcat gctggttggt
      541 gaagagcatc gcggcctcga gtacatgttc atcatgatga atatcgcgcg ctttgccgtc
      601 ggcattcagg gcatcggcgt agcggaacgc gcctgccaaa aggctgtggc gtatgccaag
      661 gagcgtgtgc aatctcgcga tctcgccgga tcggcgggtg cggcgaccat catccaccag
      721 ccggatgtgc gtcgcatgct gatgtcgatg cgcgcgcagg tggaagcggc gcgcgcgctg
      781 gcctatgttg ctgctgcggc atccgatgtc ggtcatcacc atcccgacga ggcgacgcgc
      841 cgggcgaatc aggcctgtta tgaatatctg gtgcctatcg tcaaaggatg gtcgaccgaa
      901 ttatctatcg atgtcgcctc gaccggcgta caagtgcatg gtggcatggg tttcatcgaa
      961 gaaaccggcg tcgcgcagta ttatcgcgat gcgcgcattc tgagtattta cgaagggact
     1021 accgcgatcc aggccaatga tctggtcgga cgtaaaactg tgcgtgacgg cggcgcggtc
     1081 gctagaggca tcattgcgca gatacgcaag accgaagcgg cgctgggcgc tgtttctggt
     1141 gaccatggcg atgatctgca agccatcggc aagcgcctgg tcgatggctc gcgcgcgctg
     1201 gaagaggtcg tggattacgt tgtgaccggc atcagatccg acatcaaggc ggttttttcc
     1261 ggcagtgtgc cttatctgaa gctggcaggt gtggtgctgg gcggctggca gatggcgcgc
     1321 gctgcgctgg ttgcggtcgg ccggctcagg gcgggcgagg gtgacgcgca gttctacgag
     1381 gccaaaattg ctaccgcgcg cttttacgcc gatcatgtgc tgactcaggc atcgtcgctg
     1441 cgcacttcaa tcgtcgaagg aagcgctggt gtgctggcgt tgacggaaga tcagttctga
     1501 actgaccgga tggcgcttca gccgtaagcg ccgccggtaa acagcagatc gaagctgcgc
     1561 gccagcaggg cgatcaccgc catggcgccg atgcccagcg ccaatatcag tatcaatgcc
     1621 aggatccagt gcgatccgga ttttctgccg gaggcgggat tgtgtctggc atcccactgt
     1681 tcatcggagg tcagcccgat gcacagtgtt tcgaggaaag cgcacaacac tgacaacaca
     1741 aacagcatcc ccgcgaataa aggctggata tggctgaaaa gcgcgatgag cagcgccgaa
     1801 accggcaagc tggcggcatg cagccagccg aacaggtctt tgcggccata taaatagaaa
     1861 cgatgcatgc cgatgccgcc ggaaatggct gcgaggaagg cggtcagggt tttgttttta
     1921 tgcgtaatta tcatgaaact ggttttagcg atgcagcccc aggtgggcag gcagtcggcg
     1981 tgcgcagcac aacccatcca ccatcgcgcg gatctgctcg agatcaagca cggccgcagt
     2041 atggggatcc accatggccg catgatacac atgttcgtga ttctggttca acactgtctt
     2101 gacgatcagt tcctgcatgt tgatgtcggt gcgcatcaac gccgtgagtt gcggcggcag
     2161 tttgccgacg cgcgtcggct ggaatatgcg ctcaagtcgc tgtttgggtt gagagtcgcc
     2221 tatcgactca agccatcgac aatcagcctg ccgatgtcgg ccagcacggc gttgcgttgg
     2281 tcttccggcg ccctggtttc ggtcaggtag acggatgtca ataaaggttt gccttgcggc
     2341 ggccagatga tggcgatcgt gttggtggtg ccgtaagcgc cggaaccagt cttgtcgccg
     2401 accttccagc ccggcggcat gccagcgcgt acacggcggt ccccggtttt gttagcagcc
     2461 atccatgccg tgatctgctg gcgtgaagcc gcggacaagg cattgcccag caaaatcttt
     2521 tgcagattgc cggtcatcgc cgccggtgtc gtggtgtcgc gcgcatcgcc ggggagcgcg
     2581 gtattgagtt caggttcgaa acgatcgacc cgcgtcagat cgtcgcccag cgacttgatg
     2641 aaatcgttca atggcgtcag accacccaga ttgcgtaaga tcagattggc ggcggtgttg
     2701 tcgctcaagg tcatggcggc ttcgcagatc tgtgccagcg acaggccatc gccaccgaca
     2761 tgcttgccgg tcgtgggcga atacggcacg agcatgtcgg catcgtagac gatgcgccgg
     2821 tcgagttgct ctgcaccggc gtcaacgcgc tgcaggacag ctgatgcggc caagaatttg
     2881 aaggtgctgc acatcggaaa gcgttcatcg gcgcgatagc cccagtgcct gccgctgccg
     2941 gtgtcgagga tttccacacc gagacggcca tcgacgcttg cctccagttt gcccaagcag
     3001 gaaatgaccg aagatgactc cccagatttt ttctcttctg ccgcccacac ccctgtgacg
     3061 caagcgctca gggccgttgc agtcatagca aggaaatgcc ttctatccgt catgtttttt
     3121 ccttgcaaaa atacatatga acgccaatgt taccgcagtg taatccggat cgaggccagt
     3181 acaccttgag atcgccgcac ccgcctctat aatttcagtg aagctccatc catctacccc
     3241 catcgagcat atgcccgacg acaccatcgc gtgcaacaaa gctatgacca ggttcgacca
     3301 gcatgacagc gtctggctgt ttggatacgg ctcgctgatc ttcaaggcgg atttttcgtt
     3361 catcgaacgt cgtccggcac acatcgttgg ctgg
//
LOCUS       DASRDH010000184        10611 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_108102,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000184 DASRDH010000000
VERSION     DASRDH010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 10611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10611
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108102"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1690)
                     /gene="mrdA"
                     /locus_tag="VFA14_04230"
     CDS             complement(<1..1690)
                     /gene="mrdA"
                     /locus_tag="VFA14_04230"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HZS80617.1"
                     /translation="MTEFKNTEHELKLFRQRLFIVAILVLVCFGLLTARFIWLQIIRH
                     DSYVAQAEENRISIVPIVPDRGLILDRNGVVLARNQSAYTLEITPSKITASSLDELID
                     QLGTLVEIQAKDRRRFRKLQEESKNFESLPIRMRLTDAEVARFTAQRYRFPGVDVQAR
                     LFRQYPFGKTAAHVIGYIGRISQRDADRLANSDDEANYNGTNHIGKEGLEKSYETQLH
                     GTTGYEKVEISASGRAVRTLSRTLASPGHNLILSLDIELQKIIEEAFGDRKGALVAID
                     PATGDILAYVSQPSFDPNLFIEGIDQQTWDELNTSPDHPLINRPLSGTYPPGSIYKPF
                     MALAALELDKRKPSDSIRDPGFFWLGNHKFRDDKEGGHGLVDMYKSIVASCDTYYYML
                     ANDMGVDTIHDFMKPFGFGQITGIDLEHERKGLLPSTHWKNNTYRKLEQRKWYAGETI
                     SLGIGQGYNAFTPIQMAHATATLANDGVVMKPHLVKMIEDGITHERTITVPKASYRIP
                     LQKENIDIIKQAMIGVNKEGTGARAFAGAEYTSAGKTGTAQVVSIKKNEKYDTSR"
     gene            complement(1704..2216)
                     /gene="mreD"
                     /locus_tag="VFA14_04235"
     CDS             complement(1704..2216)
                     /gene="mreD"
                     /locus_tag="VFA14_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HZS80618.1"
                     /translation="MNDLRHILLPASPLFIAFSLSIAFLLNLLPWGQWIGIPDFVALV
                     LVFWAIHEPRKIGFSITFMMGLLMDIHQATLLGENALAYTLLSYFSIMIHRRVLWFPT
                     GIQALHILPLLLFTQIVQLVVHLMVSGKLPHWYYFSESFVCALLWPLVTWLLLAPQRR
                     ALNRDDNRPI"
     gene            complement(2213..3157)
                     /gene="mreC"
                     /locus_tag="VFA14_04240"
     CDS             complement(2213..3157)
                     /gene="mreC"
                     /locus_tag="VFA14_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013236580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HZS80619.1"
                     /translation="MDIPPLFKQGASARARAGFFSLIAIIMLMVDMRMHTLTMVRQVV
                     GAMLYPLQAVALMPRDAVYQVSGYFRSLTALQKENDVLRRQQTMNAWSLQQERQMAAE
                     NTRLRNLLNMQQRIQSKSLVGEILYDARDPFTRKIILDRGSRQGVAPGQPVIDDIGIV
                     GQVTRVFPLTSEVTLLTDKDQAIPVQVLRNGLRSVAYGRGQSGVLDLRFIAANADIQK
                     GDILVTSGIDGVYPAGLPVASVLQVETKSTDAFAHIICLPKAGVDHHKQLLILLLEQN
                     ITPHPKPENAAGKDNKLPKRHLRDNASNDTVPARNTAQ"
     gene            complement(3272..4315)
                     /locus_tag="VFA14_04245"
     CDS             complement(3272..4315)
                     /locus_tag="VFA14_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004628293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HZS80620.1"
                     /translation="MFGFLRSYFSNDLAIDLGTANTLIYGRSQGIILDEPSVVAIRQQ
                     GGPNGKKTIQAVGKEAKQMLGKVPGNIEAIRPMKDGVIADFTVTEQMLKQFIRMVHDS
                     RLFRPSPRIIICVPCGSTQVERRAIRESALGAGASQVYLIEEPMAAAIGAGLPVSAAT
                     GSMVVDIGGGTTEVGIISLGGMVYKGSVRVGGDKFDEAIANYIRRNYGMLIGEQTAEM
                     IKKEIGSAFPGSEVKEIEVKGRNLSEGIPRAFTISSNEILEALTDPLNNIVSAVKNAL
                     EQTPPELGADIAEKGMLLTGGGALLRDLDRLLMEETGLPVIVAEDPLTCVVRGSGMAL
                     DLMDKLGSIFASE"
     gene            4589..4891
                     /gene="gatC"
                     /locus_tag="VFA14_04250"
     CDS             4589..4891
                     /gene="gatC"
                     /locus_tag="VFA14_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011979421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HZS80621.1"
                     /translation="MTLTLSDVKRIANLSRLELTDQQVGQTLEKLKGIFSLVEQMKAI
                     DTTGIEPLSHPIAALTADLSLRLREDEVTETDHRDSYQQPAPATQDGLYLVPKVIE"
     gene            4939..6405
                     /gene="gatA"
                     /locus_tag="VFA14_04255"
     CDS             4939..6405
                     /gene="gatA"
                     /locus_tag="VFA14_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013236578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HZS80622.1"
                     /translation="MHTKTIKELSTLLQARKISATELATLFLDRIDRSDLNAFLHVDC
                     TLTLAQAAQADRCIAGNEITPLTGIPVAHKDIFVTRGWRTTAGSKMLENYISPFDAAV
                     VEQLKIAGAVTLGKLNCDEFAMGSGNENSYFGAVRNPWDKNAVPGGSSGGSAAAIAAR
                     LTPAATATDTGGSIRQPASLCGVTGIKPTYGSVSRYGMIAFASSLDQGGPIARTAEDC
                     GLLLNVMTGFDQRDSTSIARPQEDFTRLLDESLQGLRIGVPKEFFGAGLAIDVEQAVC
                     AALKEYEKLGAALVEISLPKTGLSIPVYYVIAPAEASSNLSRFDGVRYGHRAAAYRDL
                     ADMYRKSRAEGFGDEVKRRILIGTYVLSHGYYDAYYLQAQKIRRLIAQDFQHVLTGPN
                     RQCDVIMGPVSPTVAWDLGDKTDDPVASYLADIFTLSTSLAGLPGMSIPCGFGQGEKN
                     GRRPVGLQIIGNHYDEARLLNVAHRYQQATDWHLRMPA"
     gene            6454..7917
                     /gene="gatB"
                     /locus_tag="VFA14_04260"
     CDS             6454..7917
                     /gene="gatB"
                     /locus_tag="VFA14_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014008098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HZS80623.1"
                     /translation="MQWEVVIGLETHAQLATASKIFSGASTRFGAAPNTQASPVDLAL
                     PGVLPALNKGAVERAIRFGLAIGATIAPRSVFARKNYFYPDLPKGYQISQFEIPVVQG
                     GKISFILEKDGKAEIRTVQLTRAHLEEDAGKSLHEDYQGMTGIDLNRAGTPLLEIVTE
                     PDMRSAAEAVAYAKALHSLVMWLGICDGNMQEGSFRCDANVSVRPLGRQELGTRCEIK
                     NLNSFRFMEDAINYEVRRQIELIDDGGAVVQETRLYDPDNKETRSMRNKEDAQDYRYF
                     PDPDLPPLVIAQEWIEHVRANMPELPDAMRERFVSDYALPGYDTVVLTQSKAMASYFE
                     AVVAAAGKEQAKAAANWLMGDVASTLNRADIDIAAAPVSAVQLALLLKRIADGTISSK
                     IAKEVFAGMWEAQSDKDTLADDIIDARGLKQISDSGALEAIVDQVLAANEKSVAEFLS
                     GKEQAINALIGQAMKLSRGKANPAQLTQLLRKKLGSC"
     gene            complement(7936..8778)
                     /gene="metF"
                     /locus_tag="VFA14_04265"
     CDS             complement(7936..8778)
                     /gene="metF"
                     /locus_tag="VFA14_04265"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008332817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HZS80624.1"
                     /translation="MTQHNFSIEFFPPKTPEGTEKLRATRARLAELRPKYFSVTFGAG
                     GSTQQGTLDTVLEIMREGHVATPHLSCIGSSHAALRDILTEYQSHGINKLVALRGDLP
                     SGFGAIDIAGGECRYASELVEFIRAETGDWFHIEVGAYPEMHPQARSPRDDIQHFARK
                     VQAGADAAITQYFYNADAYFNFVDEAQKLNVNAPIVPGIMPITNYAQLMRFSDMCGTE
                     IPRWIRLRLASYGDDAESIRAFGLDVVTQLCEQLLEGGAPGLHFYTLNQAGATMAIWQ
                     RLLG"
     gene            complement(8819..9172)
                     /locus_tag="VFA14_04270"
     CDS             complement(8819..9172)
                     /locus_tag="VFA14_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008332818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HZS80625.1"
                     /translation="MRLFALWLINALALLAVPYLMHSVQVDSFGSALIAALILGFVNT
                     LVRPVLVVLTLPVTLLTLGLFIFVINGLMFWIVAQLVGGFHVAGFWSAVGGAVLYSIV
                     SWALSTLLLSSAHSQ"
     gene            complement(9252..>10611)
                     /gene="ahcY"
                     /locus_tag="VFA14_04275"
     CDS             complement(9252..>10611)
                     /gene="ahcY"
                     /locus_tag="VFA14_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HZS80626.1"
                     /translation="WGHKEIKIAETEMPGLMAIREEFAAAQPLKGARIAGSIHMTIQT
                     SVLIQTLEALGAKVRWASCNIYSTQDHAAAAIADKGTPVFAFKGESLADYWEFTHRIF
                     EWPDAEDQQANMILDDGGDATLLLHLGARAERDASVLNDPTSEEEICLFNSIKKRLAG
                     HKHWYSSRLAQIKGVTEETTTGVHRLYQMHKEGKLAFPAINVNDSVTKSKFDNLYGCR
                     ESLVDGIKRATDVMIAGKIAVIAGYGDVGKGSAQAMRALSAQVWVTEIDPICALQAAM
                     EGYRVVTMDYAAAHGDIFVTCTGNYHVITHEHMQQMKDQAIVCNIGHFDNEIEVAALK
                     RYSWENIKPQVDHIIFPDGKRIILLAGGRLVNLGCATGHPSYVMSSSFANQTIAQIEL
                     YVNTRNYPVGVYTLPKHLDEKVARLQLKKLNAQLSTLTAEQAAYIGVKPEGPYKPEHY
                     RY"
BASE COUNT         2229 a         2866 c         3170 g         2346 t
ORIGIN      
        1 gacgactggt gtcgtatttt tcgttcttct tgattgatac cacctgtgcc gtgccggtct
       61 tacctgccga ggtgtattca gcgccggcga acgcgcgcgc accagtacct tccttgttga
      121 cgccgatcat ggcttgtttg atgatgtcga tattttcttt ctgcaaggga atgcgataac
      181 ttgccttcgg cacagtgatg gtacgctcat gggtgatgcc gtcttcaatc atcttgacca
      241 gatgcggttt catcacgacg ccatcgttgg ccagcgttgc tgtggcatgt gccatctgta
      301 tcggcgtgaa ggcgttatag ccctgcccga tacccaatga aatagtttcg ccggcatacc
      361 atttgcgttg ctccagcttg cggtaggtat tgtttttcca gtgagtggac ggcagcaagc
      421 ctttgcgctc gtgctcgagg tcgatgccgg tgatctggcc gaagccgaat ggtttcatga
      481 aatcatgaat cgtgtcgacg cccatatcat tggccagcat gtagtaatag gtgtcgcagg
      541 acgctacgat ggatttatac atgtcgacga ggccgtgtcc accctccttg tcgtcgcgga
      601 atttatggtt gccgagccag aaaaaccccg gatcacgaat cgagtcggat ggcttgcgtt
      661 tatccagttc cagcgctgcc agggccatga acggtttata aatcgaaccg ggtgggtagg
      721 tgcctgaaag cggacggttg atcagcggat ggtcgggcga ggtgtttagt tcatcccagg
      781 tttgctgatc gataccttcg atgaagaggt tagggtcgaa agacggttgc gagacatagg
      841 ccagaatatc gccagtggca gggtcgattg ccactagtgc gcccttgcgg tccccgaagg
      901 cttcttcgat gattttctgt agttcgatat cgagcgacag gattagattg tggccaggac
      961 tggccagggt gcgtgagagc gtgcgcacgg cgcggccact ggcggaaatt tctacttttt
     1021 catagccggt ggtgccgtgc aactgggttt cataactttt ctccaggcct tccttgccga
     1081 tatggttggt gccgttatag ttggcttcat cgtcagagtt ggcgaggcga tccgcgtcac
     1141 gctggctgat acggccgata tagccgatca cgtgggcggc agtcttgccg aaaggatact
     1201 ggcggaacag ccgcgcctgg acatcgacgc cagggaaacg gtaacgctgc gcggtaaatc
     1261 tggcaacctc tgcatctgtc aggcgcatgc ggattggcag gctctcgaaa tttttcgatt
     1321 cttcctgtag tttacggaaa cggcgccggt ctttggcttg aatctcgacc agtgtgccca
     1381 gttgatcgat cagctcgtcg agactgctgg ccgtgatttt ggacggtgtg atttccagtg
     1441 tataagcgga ctggttgcgc gccagcacca cgccgttgcg gtccaggatc agtccccggt
     1501 cgggaacaat gggtacgatc gagatacggt tttcttccgc ctgcgcaaca taggaatcgt
     1561 ggcggatgat ctgcagccag atgaaacgcg ctgttaggag gccaaaacac acgagtacca
     1621 gaatcgcgac gatgaacaga cgttgccgga agagcttgag ctcatgctca gtgtttttga
     1681 attctgtcat gctgccctgc tcatcaaatc ggacggttgt catcgcggtt gagcgcgcgt
     1741 cgttgtggtg cgagcagcaa ccaggtgacc agtggccaca agagcgcgca gacgaagctt
     1801 tcactgaagt agtaccaatg cggaagcttg ccgctaacca tcaggtgtac tactagttgt
     1861 acgatctgtg taaacagcaa tagcggtagg atgtgcagcg cctggatgcc ggtggggaac
     1921 catagtacgc ggcgatggat catgatcgaa aagtaggaca gcagcgtgta ggccagtgcg
     1981 ttttcgccca gtagcgtggc ttgatggata tccatcagca ggcccatcat gaaagtgatg
     2041 ctgaagccaa tcttgcgcgg ttcgtggatg gcccagaaca ccagcactag cgccacgaag
     2101 tcaggtatgc cgatccattg gccccaaggc agaagattga gcaggaaggc gatgctcagg
     2161 ctgaacgcga tgaacagtgg gctggccggt aacagaatgt ggcgcagatc gttcattggg
     2221 cggtgttcct ggctggcacg gtatcattgc ttgcgttgtc gcgcaggtgg cgtttgggca
     2281 gcttgttgtc cttcccggcg gcgttttcag gtttgggatg cggcgttatg ttttgttcca
     2341 gcaacagaat caataattgt ttgtgatgat cgacgccagc cttaggcagg cagatgatat
     2401 gggcaaaagc atcggtcgac ttagtttcga cctgtaacac gctggccacc ggcaagccgg
     2461 ctggatacac gccgtcgatg ccggacgtca ccaggatatc gcctttctgg atatcagcat
     2521 tggctgcgat gaagcgtaag tccagcacac ccgactggcc gcggccataa gccacgctgc
     2581 gcaaaccatt gcgtaagacc tgcacaggaa tcgcctggtc cttgtcagtt aacaaggtga
     2641 cttcggaggt caatggaaat acacgcgtca cctgcccgac gatgccgata tcgtcgatca
     2701 ctggctggcc gggcgcaacg ccttgccggg agccgcgatc gaggatgatc ttgcgggtaa
     2761 aggggtcgcg tgcgtcatag aggatttcac cgacgaggga tttactctgg atgcgctgct
     2821 gcatgttcag caaattgcgt aaccgggtgt tttctgccgc catctggcgt tcttgctgca
     2881 acgaccaggc attcatggtc tgctggcggc gtaggacgtc gttttccttt tgcagtgctg
     2941 tcagcgagcg gaaatagccg ctgacctgat acaccgcgtc gcgcggcatc agcgctacag
     3001 cctgaagcgg gtacagcatg gcaccgacca cttgtcgtac catggtgaga gtgtgcatgc
     3061 gcatatcgac catcagcatg atgatcgcaa tcaaggaaaa aaaaccggcg cgtgcgcgtg
     3121 ctgaagcgcc ttgcttgaag agtggcggga tatccatgga tgtcagatcg aaaccaatgc
     3181 cagaggcacc gaacagcgcg gcaactgacg gataccggga gaattgtggt cgtttcaatt
     3241 cctcaaggtt aggcagactc ccgtaacgga attattcgct ggcgaagatc gaacctagtt
     3301 tatccataag gtcgagcgcc atgccggaac cgcgcaccac gcaggtcagc gggtcttcag
     3361 cgacgatcac tggcaagccg gtttcttcca tcagcaggcg gtccagatcg cgcagcagcg
     3421 cgccgccgcc ggtgagcaac atgccttttt cggcaatgtc ggcgccgagt tccgggggcg
     3481 tctgctccag cgcattcttg actgcggaga cgatgttatt gagcgggtcg gtcagcgcct
     3541 ccaggatttc gttgctggag atggtaaacg cgcgtggaat gccttcggac aggttacggc
     3601 ccttgacttc tatttccttg acttcggaac ccgggaaggc agaaccgatt tctttcttga
     3661 tcatttctgc agtctgttcg ccgatcagca tgccgtagtt gcggcggatg tagttggcga
     3721 tggcttcgtc gaatttgtcg ccgccgacgc gtaccgaacc cttgtatacc atgccgccca
     3781 gcgagatgat gccaacttcc gtggtgccgc cgccgatgtc gactaccatc gagccggtgg
     3841 cagctgacac cggcaggccg gcgccgattg ctgctgccat tggttcttcg atcagataga
     3901 cctgcgacgc gccagcgcct agcgccgatt cacggatggc gcggcgttcc acctgggtag
     3961 aaccgcaggg aacacagatg atgatgcgag gggaagggcg gaatagcctg gaatcatgca
     4021 ccatgcggat gaattgtttc agcatctgtt cggtaacagt gaagtcggcg atcacgccat
     4081 ctttcatcgg acggatggcc tcgatattgc ctggtacctt gcccagcatc tgcttggcct
     4141 ctttgcctac ggcctgtatg gtcttcttgc cgttgggacc gccctgctgg cggatagcta
     4201 cgaccgatgg ttcgtcgagg atgatgcctt gactgcgtcc gtaaatcagg gtgtttgccg
     4261 ttccaaggtc gatagcgagg tcattagaga aataactacg taaaaatcca aacatgaatt
     4321 gtcctgatgc gctagtgtcg cagcgcgctg tgtaagtggg gaggaagatc ctgtatgccg
     4381 cggcgacaca aatacagcgc cagcaaagta ttagtccgac attctacctt ataatccgac
     4441 ggacaccagc aggcaaaact gcctcatgat ctttgccgga tgcccggtat ttctgtgttt
     4501 ttaccgggca tttaccgggg gtattgcagc cttgatgacg cgatttttca acatatcagt
     4561 tcttgcgccc ggtgcgcacc tttctgccat gacgctaacc ctttccgatg tgaaacgcat
     4621 tgccaatcta tcccgtctgg aattgacgga tcaacaggtc ggccagactc tggaaaaact
     4681 caaaggcatc ttttcgctgg tcgaacagat gaaagccatc gataccaccg gcattgaacc
     4741 cttgagccat ccgattgcgg cattgaccgc cgatctgtcg ctgcgattgc gcgaagatga
     4801 ggtgaccgaa accgatcacc gcgacagtta tcagcagccg gcgccagcta cccaggacgg
     4861 cctgtatctg gtgcccaaag tcatcgaata gagactgcgg cgttccgcat ctcatcgaac
     4921 cacacgcctg tcttgtccat gcataccaaa actatcaaag aactgtcgac gctattgcag
     4981 gcccgaaaaa tatcggcaac tgaactggcc acgctgtttc tagaccgtat cgaccggagc
     5041 gacctcaacg ctttcctgca tgtcgattgc acattgacgc tggcgcaagc cgcgcaagct
     5101 gaccggtgta tcgcgggcaa tgagatcacg ccgctgaccg gtattccagt tgcgcacaaa
     5161 gatattttcg tcacgcgcgg ttggcgtacc acggctggct cgaagatgct ggaaaattac
     5221 atcagcccgt tcgatgctgc cgtggtggag cagctcaaga ttgccggtgc cgtgacattg
     5281 ggcaagctca actgcgacga attcgcgatg ggctcgggca acgaaaattc ttacttcggc
     5341 gcagtcagaa atccgtggga caagaacgcc gtaccgggcg gatcgtccgg cggctctgcg
     5401 gctgccattg ccgcgcgcct gacgccggca gcgactgcca ccgataccgg cggctcgatt
     5461 cgccagccgg cttcgctgtg tggtgtcacc ggcatcaagc cgacttatgg cagcgtgtcg
     5521 cgctacggca tgattgcatt cgcgtcgtcg ctggaccagg gcggcccgat tgccaggacc
     5581 gctgaagact gcggtctgtt gctcaacgtc atgactggtt tcgaccagcg cgattcgacc
     5641 agcatcgcac gtccgcagga agatttcacg cgtttgctcg atgaaagtct gcaaggcttg
     5701 cgtattggtg tgccgaaaga attcttcggt gcggggctgg ccattgatgt cgagcaagcc
     5761 gtgtgcgccg cgctcaagga gtatgaaaaa ctcggcgctg cgctggtcga gatttcactg
     5821 cccaaaaccg gcttgtccat ccctgtgtat tatgtgatcg caccagccga ggcatcgtcg
     5881 aacctgtcgc gtttcgatgg cgtgcgttac ggccatcgcg ccgccgctta cagggatctc
     5941 gccgacatgt acaggaagtc gcgtgcggaa ggcttcggcg acgaggtgaa gcgccgtatt
     6001 ctgattggaa cttatgtgct gtcgcacggc tactatgatg cttactatct acaagcgcaa
     6061 aaaatccgtc gcctgatcgc acaggatttc cagcatgtgc tgacgggtcc gaaccgtcag
     6121 tgcgatgtca tcatggggcc cgtgtcgccg accgtggcat gggatctggg tgacaagacc
     6181 gacgacccgg tggccagtta tctggcggac atttttacgt tgtcgaccag cctggcgggt
     6241 ttgcctggta tgtcgattcc ttgcggtttc ggccagggcg aaaaaaatgg caggcgtcca
     6301 gtcggactgc aaatcatcgg caaccattat gatgaagcgc gcctgctcaa cgtcgcacat
     6361 cgataccagc aagccactga ctggcatctg cgcatgccgg cttgatgctg ctatgcaaaa
     6421 tctgaaacag ggaaagaatc agcggagaat actatgcagt gggaagtcgt catcggtctg
     6481 gaaacgcacg cacagcttgc taccgcatcc aaaatcttca gcggcgcgtc aacccggttc
     6541 ggcgctgcgc ccaacacgca ggccagcccg gtcgatctgg cactgccggg tgtgttgccg
     6601 gcgctcaaca agggtgcggt cgaacgcgcg atccggtttg gtctggcgat cggcgcgacg
     6661 atcgcgccgc gctcagtatt cgcgcgcaag aattactttt accccgattt gccaaaaggt
     6721 tatcagatca gccagtttga aatcccggtg gttcagggcg gcaagatttc tttcatactc
     6781 gaaaaagacg gcaaggcaga aatcaggacg gttcaactga cccgcgccca cctcgaagaa
     6841 gatgccggta aatcgctgca tgaagattat caaggcatga ccggcatcga cctcaaccgc
     6901 gccggcacgc cgctgctgga aatcgttacc gaacctgaca tgcgcagcgc cgccgaagcc
     6961 gtcgcttacg ccaaagccct gcactcactg gtgatgtggc tgggcatctg tgacggcaat
     7021 atgcaggaag gctcgttccg ttgcgatgcc aatgtctcgg tgcgtccgct cggacggcag
     7081 gagctcggca cgcgttgcga gatcaagaat ctgaattcct tccgtttcat ggaagatgcg
     7141 atcaactacg aagtgcgccg ccagatcgaa ctgatcgacg acggtggcgc cgtggtgcag
     7201 gaaacccgtc tctacgatcc cgacaataaa gaaacccgct ccatgcgcaa caaggaagat
     7261 gcgcaggatt atcgttactt ccccgatccc gacctgccgc cgctggtgat tgcgcaggaa
     7321 tggatcgagc atgttcgggc gaatatgccg gaactgccgg atgcgatgcg cgagcgcttc
     7381 gtcagcgatt acgccctgcc cggatacgac accgtcgtgc tgacgcaatc gaaggcgatg
     7441 gcgtcctatt tcgaggcggt ggtcgccgct gccggcaagg agcaggccaa agccgcagcc
     7501 aactggctca tgggcgacgt cgcctccacg ctcaaccgcg cagacatcga cattgccgcc
     7561 gcgccggtca gcgccgtaca actggcgctg ctcttgaagc gcatcgccga cggtacgatt
     7621 tccagcaaaa tcgccaagga agtattcgct ggcatgtggg aagcgcagtc ggacaaagac
     7681 acgttggccg acgacatcat cgatgctagg ggtttgaagc agatctccga cagcggcgcc
     7741 ctggaagcga ttgtcgatca ggtgttggca gccaatgaaa agtcggtggc ggagtttctt
     7801 tctggcaagg agcaagccat caacgcgctc atcggccagg ccatgaagtt gagcagaggt
     7861 aaggccaatc cggcgcaatt gacgcaatta ctacgcaaga aactgggcag ctgttgaggg
     7921 tcagggcggc agtgatcaac cgagcaggcg ctgccagatt gccatggtcg cacccgcctg
     7981 gttcagtgtg tagaaatgca ggcctggcgc gccgccttcg agcaactgtt cgcataattg
     8041 tgtgaccaca tcgagaccga aagcgcgaat cgactcggcg tcgtcgccat agctggccag
     8101 cctgagacga atccagcgtg ggatttccgt gccgcacatg tcggaaaaac gcatcagttg
     8161 cgcatagttg gtgatcggca tgatgcctgg cacgatcggc gcgttcacat tcagtttctg
     8221 cgcttcatcg acgaaattga aataggcgtc ggcgttatag aaatactgtg taattgccgc
     8281 atcggcgccg gcctgtacct tgcgtgcaaa gtgttgaata tcatctcgcg gcgatctggc
     8341 ctggggatgc atttccgggt aagcgccgac ctcgatatga aaccagtcgc cagtttccgc
     8401 tcggatgaat tcgaccagtt cgctggcata acggcattcc ccgcccgcga tatcgattgc
     8461 gccgaagccg ctcggcaaat cgccgcgcag cgcgaccagc ttgttgatgc catgtgactg
     8521 gtattccgtc aggatgtcac gcaaagccgc atgcgaacta ccgatacagg acaggtgcgg
     8581 tgtggcgaca tggccttcgc gcatgatttc cagcacggta tcgagtgtac cctgctgggt
     8641 cgaaccgcca gcgccgaacg tgaccgaaaa atacttcggc cgcagttcgg ccagtctggc
     8701 gcgggtggcg cgcagttttt ctgtgccttc gggtgtcttg ggcgggaaaa actcgatgct
     8761 gaaattatgt tgtgtcatga caatgcgatc caaaaacgat caaggtatcc atccatcatc
     8821 attgactgtg ggcggaactc agcaatagcg tggatagcgc ccaggaaacg atgctgtaca
     8881 gcacagcgcc accgacggcc gaccagaaac ctgcgacatg gaaaccgccg accagctgcg
     8941 ccactatcca gaacatcagg ccgttgatta cgaaaatgaa caggccgagt gtcagcagcg
     9001 tcaccggcag ggtcagcacg accagtaccg gacgcaccag tgtattgacg aagccgagga
     9061 tcagcgcggc aatcaaagcc gagccgaagc tgtcgacctg taccgaatgc atcaggtaag
     9121 ggacggccag caaggccagc gcattgatga gccagagagc gaacaggcgc atgaagtgtc
     9181 cttttgaacg gcgcgctcgc cggattgctg cgatgcgcgg caatccggac gataacaggt
     9241 aatgttgccg gttagtaacg ataatgctca ggcttgtaag gcccttccgg cttgacgccg
     9301 atataggcgg cttgctctgc ggtcagtgta ctcagttgcg cgttgagttt tttcagttgc
     9361 aggcgtgcga ctttttcatc cagatgcttg ggcagcgtgt agacgccgac cggatagttc
     9421 ctggtgttga cgtacagctc gatctgggcg atggtctggt tggcgaagga tgaactcatc
     9481 acgtaggaag gatggccggt ggcgcagccg aggttgacca ggcggcctcc tgccagcagg
     9541 atgatgcgct tgccgtcagg aaaaataatg tggtcgactt gcggcttgat gttttcccag
     9601 gagtagcgtt tcagcgcggc gacttcgatt tcattgtcga aatggccgat attgcagacg
     9661 atggcctgat ctttcatctg ttgcatgtgt tcgtgcgtga tcacatggta gttgccggtg
     9721 caggtgacga agatgtcgcc gtgtgcggca gcgtagtcca tcgtgacgac gcggtagcct
     9781 tccatggctg cttgcagtgc gcagatcgga tcgatttcgg ttacccagac ttgcgccgac
     9841 agggcgcgca tggcctgggc tgaacctttg ccgacatcac cgtaaccagc aatgacagcg
     9901 atcttgccag cgatcatgac gtcggtggcg cgcttgatgc catcgaccag cgactcgcgg
     9961 caaccgtaca gattgtcgaa tttggacttg gtgacggagt cattgacatt gatggctgga
    10021 aaggcaagct tgccttcttt gtgcatctgg tacaggcgat gcacgccggt agtggtttct
    10081 tcggtcacgc ccttgatttg tgccagacgc gaggaatacc agtgtttgtg gccggccaga
    10141 cgctttttga tggagttgaa caggcagatt tcttcttccg aggtcggatc gttcagtacc
    10201 gaggcgtctc tttcagcgcg ggcgcctagg tgtagcagca gcgtcgcatc gccgccgtcg
    10261 tcgaggatca tgttggcttg ctggtcttcc gcgtccggcc attcgaagat gcgatgggtg
    10321 aattcccagt agtcggccag tgattcgccc ttgaacgcga acaccggcgt gcctttgtcg
    10381 gcgattgccg cagcggcatg atcctgggtc gagtagatgt tgcacgatgc ccaacgtacc
    10441 ttggcgccca gcgcttctag cgtctggatc agcacactgg tctggatggt catgtggatg
    10501 gaaccggcga tgcgtgcgcc cttgagcggc tgggcggcgg caaactcttc gcggatggcc
    10561 atcaggccag gcatttcggt ttcggcgatc ttgatttctt tgtggcccca t
//
LOCUS       DASRDH010000185         2964 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_16834,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000185 DASRDH010000000
VERSION     DASRDH010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16834"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..512
                     /locus_tag="VFA14_04280"
     CDS             <1..512
                     /locus_tag="VFA14_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017454386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HZS80627.1"
                     /translation="GIINFQGPISNPSLHILAMRRHQDVEAGVEVSGYASQPRVRLVS
                     EPTVPDDEKLSWIMFGHGSDGSGLGQRSASNQALSFVGNFGGKKIAKDIGLDQFFIGA
                     SESGLSDGQVVHLGKAISEKLSVGYEQSLTGAASIAKATWQLSQRWSMVARTGAIDGL
                     NMLYSRRFD"
     gene            591..1982
                     /locus_tag="VFA14_04285"
     CDS             591..1982
                     /locus_tag="VFA14_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008449016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HZS80628.1"
                     /translation="MSIFRTKSLDELIQTSKRPGGLKKVLGPIDLVMLGIGAIVGTGI
                     FVLTGTGALTAGPALMLSFVIAAIACCFAALCYAEFASAIPVAGSIYTYSYATLGEII
                     AWLIGWDLILEYGLATATVSVGWSGYLQSLLSGFGLALPKGLTAAPGAVPGVTTWINL
                     PALLIMLAVSGLLSLGIRESAKLNNFMVLIKIGVVLLFILVGMHYVEPANWQPFAPFG
                     TSGILGAAAIVFFAFIGFDAVTSAAEEVKNPSRDLPIGIIGSLLVCSVLYVAVSGIMT
                     GIVPFQKFQGVDHPISLALQYTKQFWVAGFVDLGAIIGMTTVILVMAYGQSRIIFAMS
                     RDGLLPDRLSTIHPKYGTPFLTTWLTGIIFGLIAAFIPLNVLAELVNIGTLSAFCLVS
                     IAVIVLRVKRPDLPRTFRCPGVPVIPALAIILCVTLMMHLSQHTWIAFAVWMAAGIFV
                     YFLYARSRSKLNK"
     gene            complement(2050..>2964)
                     /locus_tag="VFA14_04290"
     CDS             complement(2050..>2964)
                     /locus_tag="VFA14_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006393598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HZS80629.1"
                     /translation="FDRCDVAVVTNIGMGDHLGLSYISTVEDLAVVKRVIVENVASSG
                     HAVLNAADPMVSSMAPSCPGTVIFFALDPLHPVMATHRAQGYQVVYRDGDEIVAAWDA
                     QEYRLSLNDIPLTRNGAIGFQVENAMASIAAVWALNLNWDIVCAGLRSFSGDVSTVPG
                     RFNLFDYRGATLIADYGHNPDAIQALVKAIANMPAQRRSVVISGAGDRRDIDIRQQTE
                     ILGDAFDEVVLYQDQCQRGRADGEVLALLRQGLSHARRTRKTREVYGEFLAIDMALAS
                     LQEGDLCLILIDQVEEALAHIARRVAEA"
BASE COUNT          609 a          853 c          830 g          672 t
ORIGIN      
        1 gcggcatcat caacttccag ggaccgatca gcaaccccag tctgcacatc ctagcgatgc
       61 gccgccatca ggacgttgaa gccggcgtgg aagtcagcgg ctacgccagc cagccgcgtg
      121 tgcggctagt gtcggaaccg accgtgcccg acgacgaaaa actgtcgtgg atcatgttcg
      181 gccatggcag cgacggttcc ggcctgggcc agcgcagcgc cagcaatcag gccttatcct
      241 tcgtcggcaa ctttggcggc aagaaaattg ccaaggatat cggcctcgac cagtttttca
      301 tcggcgccag cgaatccggc ctctccgacg gacaggtagt gcatctcggc aaggctattt
      361 cagaaaaact ctcggtcggc tacgaacaaa gtctgaccgg cgccgccagc atcgccaagg
      421 ccacctggca attgtcgcag cgctggtcga tggtggcgcg taccggcgcc atcgatggcc
      481 tgaacatgtt gtacagtcgc cgtttcgatt aaagcaatgc aggttcccag aatttgttta
      541 caatctgcta ctattccata aagcattatt ttaagttgac gagaaaccac atgagcatct
      601 tcagaaccaa aagtctggat gaattgatcc agaccagcaa acgcccgggt ggcctcaaaa
      661 aggtgctcgg cccaattgat ctcgtcatgc tggggatagg cgccatcgtt ggcaccggca
      721 tattcgtgct gaccggtaca ggagcattga cggccggacc tgcgctgatg ctgtccttcg
      781 tcattgctgc gatagcctgc tgtttcgcgg cgctgtgcta tgccgaattc gcttccgcga
      841 taccggtggc tggttccatt tacacttaca gctacgccac actgggcgaa atcattgcat
      901 ggctgatcgg ctgggatctg atactggaat atggactcgc caccgcaacg gtcagcgtgg
      961 gctggtcggg ctacctccag tcgctgctgt ccggcttcgg actcgctctg cccaaggggt
     1021 tgaccgcagc acccggcgca gtgcctggcg tgaccacatg gatcaatttg ccagcgctgc
     1081 tcatcatgct ggctgtgagc ggtctgctct cccttggaat acgggagtcc gcgaagctca
     1141 acaacttcat ggtgctgatc aagatcggcg ttgtgctgct tttcattctg gttggcatgc
     1201 actacgtcga accggcaaac tggcaaccat tcgctccctt cggcacatcg ggaattctgg
     1261 gagctgccgc cattgttttc tttgcattca tcggatttga tgcggtgacg tcggctgcag
     1321 aggaagtcaa aaatccatca cgagaccttc ctatcggcat cataggttca ctgctcgtgt
     1381 gctcggtgtt gtatgtagca gtctccggga tcatgacagg catcgtcccc ttccagaaat
     1441 tccagggcgt ggatcaccct atctctctgg ctctgcaata taccaagcaa ttctgggtag
     1501 caggctttgt ggacctgggc gccattattg gcatgaccac agtcatactg gtcatggcct
     1561 atggacagag cagaatcata tttgccatgt cacgtgacgg actcttgccg gatcggctgt
     1621 caacaatcca cccaaaatat ggcaccccct tcttgacgac atggctgaca ggcatcattt
     1681 tcgggctgat tgccgccttt attccgttga acgtactcgc cgaactggta aacatcggca
     1741 ccctgtctgc tttttgcctg gtttccatcg ccgttatcgt gctgcgggtg aagcgacctg
     1801 atttgcctcg cacattccgc tgtccaggag ttcccgtcat tcctgcgctg gctatcattc
     1861 tctgcgtcac cctgatgatg catttgagtc agcatacatg gattgctttt gctgtgtgga
     1921 tggcagcggg aatctttgtg tattttctct atgcacgctc caggtcgaaa ttgaacaagt
     1981 agtcgatgga atgccggaac cagctatcct gaacgactca ataacgaatt cggtttcaac
     2041 tgtccatcct caagcttcag ccacgcgcct ggcgatatgc gccagcgctt cttcgacctg
     2101 gtcgatcagg atcagacaca ggtcgccttc ctgcaatgac gccagcgcca tatcgatggc
     2161 caggaactcg ccgtagactt ccctggtttt cctggtgcgg cgggcgtggg acaggccctg
     2221 gcgcagcagc gccagcactt cgccgtcggc gcggccgcgc tggcattggt cttgatacag
     2281 tacgacttcg tcgaaggcat cgccgaggat ttcagtctgt tgacgaatgt cgatgtcacg
     2341 gcgatcgccg gcgccgctga tgacgaccga gcgacgttgg gccggcatgt ttgcgatcgc
     2401 tttgaccagc gcctggatgg catcaggatt gtgaccgtag tcagcgatca gcgtggcgcc
     2461 acgatagtcg aacaagttga agcggcccgg aacagtgctg acatcgccgc tgaagctgcg
     2521 caaacctgcg cacacgatgt cccagttcag gttcagcgcc cagacggcgg caatcgacgc
     2581 catggcgttt tctacctgga aaccgatggc accattgcgc gtcaacggaa tgtcgttcag
     2641 agacaggcgg tattcctgag cgtcccatgc ggcgacaatc tcatcgccat cgcgatagac
     2701 gacctggtat ccttgcgcgc gatgtgtcgc catgaccgga tgcagcggat cgagggcgaa
     2761 gaaaatgacc gtgccgggac aggacggcgc catgctggag accatggggt cggcggcatt
     2821 gagtacggca tggccggatg aggcgacatt ttcaacgatg acccgcttga ctacggcaag
     2881 gtcttccacc gtgctgatat aactcaaacc caggtggtca cccatgccga tattggtgac
     2941 cacagcaacg tcgcagcggt cgaa
//
LOCUS       DASRDH010000186         2923 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_363172,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000186 DASRDH010000000
VERSION     DASRDH010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2923
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363172"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1077)
                     /locus_tag="VFA14_04295"
     CDS             complement(<1..1077)
                     /locus_tag="VFA14_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HZS80630.1"
                     /translation="MGKIIGIDLGTTNSCVSVMEGGQSKVIENSEGARTTPSIIAYQE
                     DGEILVGAPAKRQAVTNPKNTIYAAKRLIGRKFDEKEVQKDISLMPYQIVAADNGDAW
                     ISVRGNKLAPPQISAEVLRKMKKTAEDYLGEEVTEAVITVPAYFNDSQRQATKDAGRI
                     AGLDVKRIINEPTAAALAFGLDKAEKGDRKIVVYDLGGGTFDVSVIEIADVDGEKQFE
                     VLSTNGDTFLGGEDFDQRIIDYVLDEFKKINGLDLSKDAIALQRIKASAERAKIELSS
                     SQQTEINEPYIAMANGAPVHLNLKITRARLESLVEELVSKTIEPCRTAIKDAGIKVAD
                     IDDIILVGGMTRMPKVQEKVKEFFG"
     gene            complement(1250..1792)
                     /gene="grpE"
                     /locus_tag="VFA14_04300"
     CDS             complement(1250..1792)
                     /gene="grpE"
                     /locus_tag="VFA14_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007881060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HZS80631.1"
                     /translation="MQDTEKQVGNASVQDVQVQEQGAETEMELTVVEKLAIAEAKIVE
                     IQDAFLRAKAETENIRRRAQDDISRAHKFAIEGFAESLLSVKDNLEMALKIDTPSVES
                     LKEGVDMTLKQLSSAFEKNRLQEIYPQAGEKLDPMKHQAISSVLAEQDANTVVTVLQK
                     GYTISDRLLRPALVTVAQGK"
     gene            complement(1920..>2923)
                     /gene="hemH"
                     /locus_tag="VFA14_04305"
     CDS             complement(1920..>2923)
                     /gene="hemH"
                     /locus_tag="VFA14_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010401699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HZS80632.1"
                     /translation="NLGTPDAPTTRAVRRYLRQFLSDPRVVEIPRAIWWCILNGIILP
                     FRSRSSAEKYAAIWTDAGSPLQVNTERQAALLQASLLERGHHVQVTYAMRYGQPALPD
                     VLDCLKAAACERILVFPAYPQYSGTTTASVFDVVFRHFARVRNVPELRLVKHYHDHDG
                     YIEALRHSVLAHWDANGRPDKLVMSFHGIPKRTLLLGDPYYCECHETARLLAQALGLS
                     EADYIVSFQSRFGKAEWLQPYTAPTLQELARQGVRRVDVMCPGFTSDCLETLEEIAME
                     AKAGFLHAGGKEFHFIACLNDAPAWGQGMMEIVEQHLAGWPTRAAALREEKKRLPAC"
BASE COUNT          588 a          868 c          785 g          682 t
ORIGIN      
        1 gccgaagaat tccttgacct tctcctgtac cttcggcata cgggtcatgc cgccgaccag
       61 aatgatgtcg tcgatgtcgg caaccttgat gccggcatcc ttgatcgcgg tgcggcatgg
      121 ctcgatggtc ttgctgacca attcttcgac cagcgattcc agcctggcgc gggtgatctt
      181 caggttcagg tggaccggcg cgccattcgc catggcgatg tacggctcgt tgatctcggt
      241 ttgctgcgat gacgacagtt cgatcttggc gcgttcagcc gatgccttga tgcgttgcag
      301 ggcgattgca tccttcgaca gatccagacc gttgatcttc ttgaactcgt cgaggacata
      361 gtcgatgatg cgctggtcga agtcttcgcc accgaggaag gtgtcgccgt tagtggacag
      421 cacttcaaac tgcttttcac catcgacatc ggcgatttca atgacggaaa cgtcgaacgt
      481 gccaccgccc aagtcataca ccacgatctt gcggtcaccc ttttcggcct tgtccaggcc
      541 gaacgccagc gccgccgcag tcggttcgtt gatgatgcgc ttgacgtcca gaccggcgat
      601 acggccggca tccttggttg cctgacgttg cgagtcgttg aagtaggcag gcacggtgat
      661 gaccgcttcg gtcacttctt ctccgagata gtcttcagcg gttttcttca tcttgcgcag
      721 cacttccgcg gagatttgcg gcggcgccag cttgttgccg cgcacgctga tccaggcatc
      781 accattatcg gcggcaacga tctgataagg catcagggag atgtcttttt gcacctcctt
      841 ttcatcgaac ttgcgaccga tcagtcgctt ggcggcgtaa atggtgttct tcggattggt
      901 gacggcctga cgtttagcag gcgcgccgac gagaatttcg ccgtcttcct gatacgcgat
      961 gatggaaggc gtcgtgcgcg cgccttcgga attttcgatc actttcgatt gtccgccttc
     1021 catgacggaa acgcacgaat tcgtcgtacc caggtcaata ccaatgattt ttcccatgat
     1081 ctttctttcc tttttgtctt tcagtccccc gcacaggggg acaacaatga ttccgatagt
     1141 tacaatatgt ggagaataat gcgactttca agcagcgcat ctcatgaatt tcacctggaa
     1201 cgcgcccacg atcccgaaaa gcagattctg tgacgtctgc tgccaccagt tacttgcctt
     1261 gcgccacagt caccagcgcc ggacgcaaca agcggtcgga aatcgtgtac cccttctgca
     1321 aaacggtcac tacggtattg gcgtcctgct ctgccagcac ggacgaaatc gcctgatgtt
     1381 tcatcggatc cagcttttcg ccggcttgcg gataaatttc ctgcagcctg tttttttcaa
     1441 aggcagacga caattgcttc aaggtcatgt cgacgccttc cttgagcgat tcgaccgatg
     1501 gcgtgtcgat cttcaacgcc atttccagat tgtccttgac tgagagtagc gactcggcga
     1561 agccttcgat cgcaaattta tgcgcacggc tgatgtcatc ctgcgcgcgg cgacggatat
     1621 tttctgtttc cgccttggca cgcagaaagg catcctgtat ctcgacgatc ttcgcttcgg
     1681 cgatagccag tttctccacg acggtcagtt ccatctcagt ctcggcgcct tgctcctgta
     1741 cctgaacatc ctgcacagat gcgtttccca cttgtttttc cgtatcttgc atattcaatg
     1801 atctccaact aatgacccgt atccggtatc gactgcccat gccgcacatg gggatgcctt
     1861 tctgaaactt caaggaaaat gccgagcctg cgccgccatg cgtattcagg agccgcattc
     1921 taacaggccg gcaggcgctt tttttcttcg cgcaatgcgg cggctctggt cggccaaccg
     1981 gccagatgct gctcgacgat ttccatcatg ccctgtcccc atgccggcgc atcgttcaaa
     2041 caggcaatga agtgaaactc cttgccaccg gcatgcagaa acccggcctt ggcttccatg
     2101 gcgatttcct ccagggtttc caggcaatcg ctggtaaagc ccggacacat gacatcgaca
     2161 cgccgcacac cctgacgcgc cagttcctgc aaggtcggcg cagtataggg ttgcaaccat
     2221 tcagccttgc cgaagcgcga ctggaagctc acgatataat ccgcctcact cagtcccagc
     2281 gcctgcgcca gcagacgcgc ggtctcgtga cattcgcaat aatagggatc gcccagcagc
     2341 agcgtgcgct tggggatgcc atgaaaactc atcaccagct tgtcgggccg gccgttggca
     2401 tcccaatgcg ccagcaccga atgcctgagc gcctcgatat agccgtcatg gtcgtggtaa
     2461 tgcttgacca ggcgtaattc gggtacattg cgcaccctgg cgaaatgcct gaaaacgacg
     2521 tcaaaaaccg atgccgtcgt ggtgccggaa tactgtggat acgccgggaa cacgagaatg
     2581 cgttcacaag ctgccgcctt gaggcagtcg agtacatcag gcagcgccgg ctggccatag
     2641 cgcatcgcat acgtcacctg cacatgatgg ccacgctcca gcaggcttgc ctgtagcagg
     2701 gcggcctggc gctctgtgtt gacttgcagc ggcgaacctg cgtcggtcca gatcgcggcg
     2761 tatttttcgg ccgatgaacg cgaacgaaac ggcaaaatga tgccattgag aatgcaccac
     2821 cagatcgcgc ggggaatttc caccacacgc ggatccgaca aaaactggcg cagatagcgg
     2881 cgcacggcgc gcgtggtcgg cgcatcgggc gtgccgagat tga
//
LOCUS       DASRDH010000187         2818 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1038464,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000187 DASRDH010000000
VERSION     DASRDH010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2818
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1038464"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="VFA14_04310"
     CDS             <1..200
                     /locus_tag="VFA14_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HZS80633.1"
                     /translation="MDLVRRIIEGAAQRLTPNGVLIVEIGNGRAAAEAAFPELDLTWM
                     TTSAGDDMVFLLTAAQLQSPV"
     gene            228..2183
                     /locus_tag="VFA14_04315"
     CDS             228..2183
                     /locus_tag="VFA14_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008328366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZS80634.1"
                     /translation="MIRFRQLSLARGVKPLLENVDLTLNPGDRIGLIGANGTGKSSLF
                     SMLRGELHADQGDIDYPAKWRMAYVAQETPALDRPAVEYAIDGDATLRHLEAQLAAAE
                     TEPESAGNGNRIAELHGALADADAYTARSRAEQLLIGLGFTLEQMSRPVASFSGGWRM
                     RLNLAQALMCPSDLLLLDEPTNHLDLNAIIWLEDWLKRYTGTLIIISHDRDFLDGVVN
                     VIVHIDERQLKPYSGNYSAFERQRAARLELAAGMLEKQARQRAHLQSFIDRFKAKASK
                     ARQAQSRMKALSKMEELAPLRAAAEFSFEFREPLSAPNPLLVMDKVDAGYRNHDPATN
                     GITETSIVSGIEFSLQPGQRIGLLGVNGAGKSTLIKTIANELKPLSGTTRFGKGLVIG
                     YFAQHQVDMLRHDESPLQHLARIAPKAREQELRNFLGSFNFSGNMASSSIAPFSGGEK
                     ARLALALIVWRRPNLLLLDEPTNHLDLETREALSMALAQFEGTLVLVSHDRYLLRATT
                     DQFLIVTDGKLCSFDGDLDDYHDWLFKTRLATVSRPGTGPGQPITTPDRREQKRLEAE
                     QRQRLALLKKPIESRIKRLEEQIARCGEKRTVIEARLADPAIYDTASKEELKMLLTNQ
                     AYCARELAQLEEDWLEQQEALERIPAR"
     gene            2325..>2818
                     /locus_tag="VFA14_04320"
     CDS             2325..>2818
                     /locus_tag="VFA14_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZS80635.1"
                     /translation="MNTIDTSATTTPTHRSRILVVDDDIRLRDLLCRYLTEQGFSVAM
                     AENAQAMNKLWMRERYDLLVLDLMLPGEDGLAICRRLRGAGDHTPIIMLTAKGEDVDR
                     IIGLEMGADDYLPKPFNPRELVARINAVLRRKGPDELPGAPSESPQSFEFGAFVLDLG
                     TRTL"
BASE COUNT          642 a          871 c          778 g          527 t
ORIGIN      
        1 gcatggatct ggtacgcaga atcatcgagg gcgcggcaca acggctgacg ccgaatggcg
       61 tgctgatcgt cgagatcggc aacgggcgcg ccgccgccga agccgctttc cccgaactgg
      121 atctgacctg gatgacaacc agcgccggcg acgatatggt atttctgctg actgccgctc
      181 agcttcagag tccggtataa cttttattct ttgatgacaa tgtcgcgatg attcgttttc
      241 ggcagctttc cctggcccgc ggcgtcaaac cgttgctgga gaatgtcgat ctcacgctca
      301 acccgggcga caggatcggc ctcatcggcg ccaacggcac cggtaaatcc agcctgttct
      361 ccatgctgcg tggtgaactg catgccgacc agggcgacat cgattatccg gccaaatggc
      421 gcatggctta tgtggcgcag gaaacacccg cgctcgaccg ccccgccgtg gaatacgcga
      481 tcgatggcga cgctaccctg cgccatctgg aagcgcaact ggccgctgcc gaaaccgaac
      541 cggaaagcgc cggaaacggc aaccgtatcg ccgaactgca tggcgcattg gccgatgcgg
      601 atgcttacac cgcgcgttcg cgcgccgagc aattgctgat cggccttggt ttcacgctgg
      661 aacagatgtc gcgcccggtg gcgagcttct ctggtggctg gcgcatgcgg ctgaatctgg
      721 cgcaagcact gatgtgtcca tctgatctac tgctgctcga cgagccgacc aatcacctcg
      781 acctcaatgc catcatctgg ctggaagact ggctcaaacg ctacaccggc accttgatca
      841 tcatctcaca tgatcgcgat tttctcgatg gtgtagtgaa tgtaatcgtg catatcgacg
      901 aacgtcaact caaaccctat tccggcaact actcggcctt cgaacgccaa cgcgcggccc
      961 gactggaact ggcggcgggc atgctcgaaa aacaggcacg ccagcgcgcc catctgcaat
     1021 ccttcatcga tcgtttcaag gccaaggcaa gcaaggcgag acaggcgcaa agccgcatga
     1081 aggcactgtc gaagatggaa gaactcgccc ccttgcgcgc ggcggcggaa ttctcattcg
     1141 aattccgcga gccgctgtca gcgccaaatc cgctgctggt catggacaag gtcgatgccg
     1201 gttatcgcaa ccacgatccc gcgactaacg ggatcacgga aacaagcatc gtctccggca
     1261 tcgaattctc gctacagccc ggtcagcgca tcggtttgct tggtgtcaac ggcgccggca
     1321 aatcgacatt gatcaaaacc atcgccaacg aactgaagcc actctccggc acgacccggt
     1381 tcggcaaggg tctggtgatc ggctacttcg cccagcatca ggtcgatatg ttgcgtcacg
     1441 acgagtcgcc gctacagcat ctggcgcgca tcgcaccgaa agcgcgcgag caggaattgc
     1501 gcaattttct cggcagcttc aacttcagcg gcaacatggc ctcatcgagc atcgcgccat
     1561 tctccggtgg cgaaaaagcg cgtctggcat tggcgctgat cgtctggcgg cgtcccaacc
     1621 tgctgctgct cgacgaaccg accaatcatc tcgatctgga aacccgcgaa gccctgagca
     1681 tggcgctggc gcagtttgaa ggcacactcg ttttggtgtc ccatgatcgc tatctgctgc
     1741 gcgccaccac cgatcaattc ctgatcgtca ccgacggcaa actatgctcc ttcgacggcg
     1801 acctcgacga ctaccacgac tggctgttca agaccagact ggcgacagtt tcccgacccg
     1861 gaaccgggcc ggggcagccg atcaccacac ccgatcgtcg cgagcagaag cgcctggaag
     1921 ccgaacagcg tcaacgcctg gcgctgctga aaaagccgat cgaatcgcgc atcaaacggc
     1981 tcgaggaaca aatcgccagg tgcggcgaaa aaagaaccgt catcgaagcc cgcctagccg
     2041 atccagccat ctacgacacc gccagcaaag aagaactgaa gatgctgctc accaaccagg
     2101 cttattgcgc cagggagttg gcgcaactcg aagaagactg gctggagcag caggaagcgc
     2161 tggaacgcat accggcccga tgatcccggc gttcaaagat gcgccagatt ctttgaaagc
     2221 aacaatattc ataagatgat ataagatgat caaagtgagg caggcaataa tttgccatgt
     2281 tcgccagctt ctctgtcgct ctatgaagac gctaccatga catcatgaat acgatcgaca
     2341 ccagcgcaac caccaccccg acccatcgat ccagaatcct ggtcgtagac gatgacatcc
     2401 ggctgcgcga cctgctgtgc cgctatctga ccgaacaggg tttcagcgtg gcgatggctg
     2461 aaaacgcgca ggcgatgaat aagctatgga tgcgcgaacg ctacgatctg ctggtgctgg
     2521 atctgatgct gccgggcgaa gatggactgg cgatctgccg tcgtctgcgc ggtgccggcg
     2581 accacacgcc gatcatcatg ctgactgcca aaggtgaaga tgtggatcgc atcatcggcc
     2641 tggaaatggg cgccgacgac tatctgccaa aaccattcaa tccgcgtgaa ctggttgcac
     2701 gcatcaacgc tgtattacgc cgcaaaggcc ccgatgaatt acccggagcg ccatcagaat
     2761 cgccgcaatc tttcgaattt ggcgcattcg tgctcgacct cggtactcgc acgctgaa
//
LOCUS       DASRDH010000188         2464 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1519365,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000188 DASRDH010000000
VERSION     DASRDH010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1519365"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1529)
                     /gene="hscA"
                     /locus_tag="VFA14_04325"
     CDS             complement(<1..1529)
                     /gene="hscA"
                     /locus_tag="VFA14_04325"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HZS80636.1"
                     /translation="MALLQISEPGMSTAPHQHRLAVGIDLGTTNSLVATVRNSIPEIL
                     SDEEGRSLLPSVVRYLPDGHVSIGCEAQAAQTTDPKNTIVSVKRFMGRSLKDIAYVEN
                     LPYDFQDVPGMVQLKTVAGVKSPVEVSAQILATLRQIAEDALGDDLVGAVITVPAYFD
                     DAQRQATKDAAHLAGLNVLRLLNEPTAAAIAYGLDHASEGTFVVYDLGGGTFDISILK
                     LTKGVFEVLSTGGDSALGGDDFDQRLFCWICEQAQLAPLSDEDTRILLIKAREVKELL
                     SSKADMRIDAVLNSGEEVHLSITAERFALLTRHLIDKTMAPCRKALRDADLSVDDVDG
                     VVLVGGATRMPHVRKAVGEFFQTTPLANIDPDKVVALGAAMQANLLAGNRAAGEDWLL
                     LDVIPLSLGIETMGGLVEKVIPRNSTIPCARAQEFTTFRDGQTALALHVVQGERELAT
                     DCRSLARFELRGIAPMMAGAARIRVTYQVDADGLLSVSAREMISGVEASITVKPSYGL
                     A"
     gene            complement(1541..2056)
                     /gene="hscB"
                     /locus_tag="VFA14_04330"
     CDS             complement(1541..2056)
                     /gene="hscB"
                     /locus_tag="VFA14_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008450506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HZS80637.1"
                     /translation="MQNYFTLFQLPQRFAVDLTALEKAYHGVQNQAHPDRFAQAGSAE
                     KRVAMQWATHANEAYQTLKNPLKRAAYLCELNGIDLETESNTAMSPAFLMQQMEWREE
                     LDDAKAGKHIAVLEQIDGSLRNARKDTIARIETLLDAADFAGAAQLVRQLMFLERFGE
                     EIGNAFSLIEA"
     gene            complement(2189..>2464)
                     /gene="iscA"
                     /locus_tag="VFA14_04335"
     CDS             complement(2189..>2464)
                     /gene="iscA"
                     /locus_tag="VFA14_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018417526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HZS80638.1"
                     /translation="MVRRGKGIGLRLGVRTTGCSGLAYKLEYVDEEVPEDQVFESHGI
                     RVFVDPRSLPYIDGTELDFAREGLNEGFRFRNPNVKDECGCGESFRV"
BASE COUNT          558 a          761 c          651 g          494 t
ORIGIN      
        1 tcggccagcc catacgaagg cttgaccgtg atcgaggctt ccacgccaga gatcatctca
       61 cgcgccgata ccgacagcaa accatcggca tcaacctgat aggtcacgcg aatgcgcgca
      121 gcgcctgcca tcatcggcgc aatgccacgc agctcgaaac gcgccagcga acggcaatcg
      181 gtggccagct cgcgctcgcc ttgcaccaca tgcagcgcca gcgcagtctg cccatccctg
      241 aaggtcgtga actcttgcgc gcgcgcgcaa ggaatcgtcg aattgcgcgg gatcactttc
      301 tccaccaggc ctcccatggt ctcgataccg agcgacagtg gaatcacgtc cagcagcagc
      361 cagtcttcgc cggcggcgcg gttgcccgcc aacagattcg cctgcatggc tgcgcccagc
      421 gccaccactt tgtctggatc gatattggcc agcggcgtgg tctggaaaaa ttcgccgact
      481 gctttgcgca catgcggcat gcgcgtcgcg ccaccgacca acacgacgcc gtctacatca
      541 tccacggaca aatcggcgtc acgcaacgcc ttgcggcatg gcgccatggt cttatcgatc
      601 agatgcctgg tcaacaaggc aaatctttct gccgtgatcg acagatgcac ttcttcaccc
      661 gaattgagta cggcatcgat gcgcatatca gccttggaag aaagcaactc cttcacttca
      721 cgcgccttga tcagcaaaat acgggtgtct tcatccgaca gcggcgccag ttgcgcctgc
      781 tcacatatcc agcaaaacag gcgctggtcg aaatcgtcac cacccaatgc ggaatcgccg
      841 cctgtcgata gcacttcaaa cacacccttg gtcagcttga gaatcgaaat atcgaaggtg
      901 ccgccgccca gatcgtagac cacaaacgtc ccttcagagg catggtccag accataggca
      961 atcgccgccg cggtcggttc gttgagcaag cgcaggacat tcaggccggc cagatgcgca
     1021 gcatccttag tggcctgacg ctgggcatcg tcaaaatagg caggcacagt aatcaccgca
     1081 ccaaccagat cgtcgcccaa tgcatcttca gcaatctggc gcaaggtagc aagaatctgt
     1141 gccgatactt ccaccggact ttttacgccg gccaccgtct tcagttgcac catgcctggc
     1201 acatcctgga agtcataagg aagattttcg acgtaagcga tatctttcag gctacgcccc
     1261 atgaagcgct tgaccgagac aatggtattc tttggatcgg tcgtctgcgc ggcctgcgcc
     1321 tcgcagccga tgctgacgtg accgtcaggc aggtaacgca ccaccgacgg caacaggctg
     1381 cgcccctctt catcgctgag gatttcggga atgctgttgc gcacggtagc gaccagcgaa
     1441 ttggtggtgc cgagatcgat gccgaccgcc aaccggtgct gatgcggcgc agtggacata
     1501 cccggttcgg agatttggag aagagccatg atcgatgtat tcaagcttcg atcagggaaa
     1561 aggcattacc gatctcttcg ccaaatcttt cgagaaacat cagttggcgc accagttgcg
     1621 ccgcaccggc aaaatcggcc gcatcgagta gtgtttcgat gcgagcaatg gtatctttcc
     1681 tggcgttgcg caagctgccg tcgatctgct ccaggacagc gatatgttta cccgccttgg
     1741 catcatccag ctcttcgcgc cactccatct gctgcatcag gaacgccggc gacatggccg
     1801 tattggattc tgtttccaga tcgatgccat tgagttcgca caaataagcg gcgcgcttga
     1861 gcggattctt gagcgtttga taggcttcgt tggcatgcgt ggcccattgc atggcgacac
     1921 gcttttcagc gctgcccgcc tgcgcgaacc tgtccggatg cgcctggttc tgcacaccgt
     1981 gatacgcctt ttccaatgcc gtcagatcga cggcaaagcg ttgtggcaac tggaaaagtg
     2041 tgaagtaatt ctgcatacgt cgtacaaaca ggtgcaaaag gggaaaatta aaaagcctat
     2101 tcggacttca acagccctgg aagggaagaa gtgcgctggc tattgcggca aacagcgctc
     2161 atccaggcaa acgctgtcga cctcagcctc agacacggaa gctttcacca caaccgcact
     2221 cgtccttgac attcgggttc cggaaccgga atccttcgtt caatccttcg cgggcgaaat
     2281 cgagttcggt gccatcgata tagggcagac tcctgggatc gacaaagaca cgaatgccat
     2341 gcgattcgaa cacctggtcc tcaggcacct cctcgtccac atattccagc ttgtaagcca
     2401 ggccggaaca accggtcgtg cgcacaccaa gacgcagacc gatacctttg ccacggcgta
     2461 ccat
//
LOCUS       DASRDH010000189         3688 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1045048,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000189 DASRDH010000000
VERSION     DASRDH010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 3688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3688
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1045048"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            129..791
                     /locus_tag="VFA14_04340"
     CDS             129..791
                     /locus_tag="VFA14_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HZS80639.1"
                     /translation="MNDLIQTVAVYALPVLFAITLHEAAHAYAAKYFGDDTAYALGRM
                     SLNPLQHIDLFGTIVIPVLLYFATSGAFLFGYARPVPLDFSRLRRPKRDMAWVALAGP
                     AANFAMALIWTLVIYALAAGNVEEKFFMLMAKAGVLTNLVMFAFNLFPIPPLDGGRIL
                     TSLLPDRYAHRFARIEPYGFFIVMALVLLKVIYVWWMAPVMYLAASLLQLITSPLSLF
                     FD"
     gene            800..1996
                     /locus_tag="VFA14_04345"
     CDS             800..1996
                     /locus_tag="VFA14_04345"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HZS80640.1"
                     /translation="MYPDRVVSGMRPTGALHLGHYHGALKNWIKLQSELPCLFFVADW
                     HALTTHYDDPSIIETSTWEMVIDWLAAGVDPSQSTLFIQSRILEHAELHLLLSMSTPL
                     GWLERVPTYKDQQEKLSDRDLATYGFLGYPLLQAADVLIYRASQVPVGEDQVPHIEMM
                     REIARRFNHLYGKEKSFEEKAREAVRKLGSKRARLYLELRTQFQEQGNEEALEQARAM
                     LDDTQNLSIIDRERLFGYLEGSRKLILTEPQALLTEAARLPGLDGAKMSKSYGNAIAL
                     REDKESVTRKIRTMPTDPQRVRRTDPGDPGKCPVWQFHLVYSDDAVRQWVDKGCRVAG
                     IGCLECKQPVIDAVLKEQQPMLERAQQYIDDPALVRAIIADGCDSARKLAQDTMRDVR
                     EAMGLE"
     gene            2212..>3688
                     /locus_tag="VFA14_04350"
     CDS             2212..>3688
                     /locus_tag="VFA14_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZS80641.1"
                     /translation="MLSACASLWQPGGTDVAMAAGSKKPTLAQTSPVRKGQEKARVQQ
                     PEDALPTPVLSDEILFKILSAEIAFQRGQWQAAYVALLGTAQQTRDPRLAKRAAEMAL
                     MVRSTKETLAAVRLWTKLAPHSDEALQNYLGLLMLDGDPSEIQPLLTYRLAEAAVQMR
                     GPMILRIQRLLSRAKDKGVAFAILQHIVAPYPMLVESHLALAQGAFANRDAIRARAEA
                     RQALELRPDSELAILTLAQVTSDNNEALKLVSGFLKKYPDAREVRIAYARGLIEQKQY
                     EQARDQFQTLLKDDQDDLTTLFALGILNVQTNQLQEAETYLKHYLDVLMTQQDDERDP
                     TQALLLLSQIAEQRHDIPSALKYLEQVELGEAYIDTQIRRAQLMSSSGNLDGARKVLQ
                     QAWPNTDDDRELLQLIQGEGRILRDNQRYQDAANVLAAGLKRFPENTDLLYDYAMATD
                     KTGNYKDMESALRKIIQLAPNNQHAYNALGYSLADRNIRLPE"
BASE COUNT          820 a         1099 c         1012 g          757 t
ORIGIN      
        1 gaccaccgtc attgacctca gcgatgatga cgggccgatg ctgatgcggc aaggacgggg
       61 tgatgccgca ccctttggct tgtgatttcc cacggctcgc cttcgtctct ctgctagaat
      121 tcgcctccat gaatgatctg atccagactg tcgccgtgta tgcgctcccg gtactgttcg
      181 cgattaccct gcatgaggca gcccatgctt acgcggcaaa gtatttcggc gacgacaccg
      241 cctacgcgct ggggcgtatg agcctcaatc ctctccagca tatcgacctc tttggcacca
      301 ttgtcattcc ggtgttgctg tatttcgcca ccagtggtgc ttttttgttc ggttacgcca
      361 ggccggtgcc tcttgatttc agcaggctgc gtcggcccaa gcgcgacatg gcctgggtcg
      421 cgctggccgg tcctgccgcc aacttcgcga tggcgctgat ctggaccttg gtgatttatg
      481 ccttggcagc aggcaatgtc gaagaaaaat ttttcatgct catggccaag gccggcgtgt
      541 tgaccaatct ggtgatgttt gcgttcaatc tgttcccaat tccaccgctc gacggcggcc
      601 gcatactgac aagtctgctg ccggaccgct atgcgcatcg attcgcacga atcgagccat
      661 acggtttctt tatcgtgatg gcgctggtct tgctcaaagt gatctacgtc tggtggatgg
      721 cacctgtcat gtacctggca gcgagcctgt tgcaactgat tacctcgccg ttatccttat
      781 tttttgacta aaacacacca tgtaccctga tcgcgtcgtc tccggcatgc gccctacggg
      841 cgcactgcat ttgggccact atcatggcgc cctgaaaaac tggatcaagc tacagtccga
      901 actgccttgc ctgttttttg tagctgactg gcatgcgctg acgacacact acgatgaccc
      961 cagtatcatc gaaaccagca cctgggaaat ggtgatcgac tggctcgccg ccggcgtcga
     1021 tccttcccaa tcgacgttgt tcatccagtc gcgcatactg gaacacgccg aactgcatct
     1081 gctgctgtcg atgtccaccc ctttgggctg gctggagcgt gtgccgacct acaaggacca
     1141 gcaggaaaaa ctctcagacc gcgatctggc gacctacggc ttcctcggtt atcccttgct
     1201 gcaagccgcc gacgtgttga tttaccgcgc cagccaggtg ccggtcggtg aagaccaggt
     1261 tccgcatatc gagatgatgc gcgagatcgc gcgccgcttc aaccatctgt acggcaagga
     1321 aaaaagcttc gaagaaaaag cgcgcgaggc cgtcaggaaa cttggcagca aacgcgccag
     1381 gctatatctg gaactgcgca cgcaatttca ggagcagggc aacgaagaag cgctggagca
     1441 ggccagggcc atgctcgacg atacacaaaa tctttctatc atagaccgtg agcgcctgtt
     1501 cggttatctg gaaggcagcc gtaaactgat cctgaccgag ccgcaagcct tgctgacgga
     1561 agctgctcgc ctgcccggtc tggatggcgc caagatgtcc aagagctacg gcaatgcgat
     1621 tgcgttgcgc gaagataagg agtccgtcac cagaaaaatc cgcaccatgc cgaccgatcc
     1681 acagcgcgtg cgccgaaccg atcccggcga tcccggaaaa tgcccggtct ggcaatttca
     1741 tctggtgtat tcggatgatg ctgtccggca gtgggtcgac aagggctgcc gtgtggccgg
     1801 catcggttgt ctggaatgca aacagccggt catcgacgcc gtcctgaaag aacagcagcc
     1861 gatgctcgag cgcgcccagc aatacatcga cgatccagcg ctggtgcgcg ccatcatcgc
     1921 cgatggctgc gacagcgccc gcaagctggc gcaggacacc atgcgtgacg tgcgcgaagc
     1981 catgggtctg gagtagattg acagccataa aattcctggg aaccaagtca ggacacccac
     2041 acaaacgcag tgtgcgggag atgatcagtg caatttctgt gttgagaaag tgggttgctt
     2101 acaccaaaaa cacgccattg tgcgtaaaat taacatctac tcgttggttt actagcaaat
     2161 tacccagggt ctactttgaa aaaatctctc gccattgtca tgctctccct catgctttct
     2221 gcatgcgcaa gcctgtggca acctgggggg accgatgttg caatggcggc gggcagcaaa
     2281 aagccgaccc tggctcagac ctcacctgtc cgcaaaggac aggagaaagc cagggtacag
     2341 cagccggaag atgcattgcc gacacccgtg ctgagcgatg aaatcctgtt caagatactg
     2401 agtgccgaaa tcgcctttca gcgtggccaa tggcaggctg cctatgtggc cttgctgggc
     2461 actgcacagc aaacccgcga tccgcgcttg gccaagcgcg ccgctgaaat ggcgttaatg
     2521 gtccgttcca cgaaagaaac attggcagca gtacgcctgt ggacgaaact ggcgccgcat
     2581 tcggatgagg cgctacagaa ttatcttggc ttactcatgt tggacggcga tccctccgaa
     2641 atacagccgc tcctcacata tcgcctggcc gaggccgctg tgcagatgcg cggcccgatg
     2701 atcctgcgca tccagcgctt gttgagccgt gccaaagaca agggcgttgc tttcgctatc
     2761 ctgcaacaca tcgtcgctcc ctatccgatg ctggtcgaaa gccatctggc gctggcgcag
     2821 ggcgcctttg ccaatcgcga cgccatacgt gcgcgcgctg aagccaggca ggccctggag
     2881 ctccgccccg attcggaact ggcgatcctg acgctggcgc aagtcacatc ggacaacaat
     2941 gaagccttga aactggtgtc cggcttcctc aagaaatatc ccgatgcccg tgaagtccgc
     3001 attgcctatg cccgtggtct gatcgagcaa aaacagtatg aacaggcgcg cgaccagttc
     3061 cagaccttgc tcaaggatga tcaggacgac ctcactacgc tgttcgcgct aggcatcctg
     3121 aatgtccaga ccaatcaact gcaggaagcc gaaacctatc tgaaacacta tctcgatgtg
     3181 ctgatgacac agcaggacga cgaacgtgat ccaacgcaag ctctgctgct gctgtcgcag
     3241 atcgccgaac agcgccatga catcccatcc gcgctgaaat acctggagca ggtcgaactg
     3301 ggagaagcct atatcgatac gcaaatccgc cgcgcccaat tgatgagcag cagcggtaac
     3361 ctcgatggcg cccgcaaagt attgcagcaa gcctggccga ataccgacga tgatcgcgag
     3421 ttgctacaac tgatacaggg cgaagggcga atcctgcgcg acaaccagcg ctatcaggat
     3481 gccgccaatg tgctggcggc cggcctgaag cgctttccgg agaacaccga cctgctgtac
     3541 gactatgcca tggcgaccga caagaccggc aattacaagg acatggaatc cgccttgcgc
     3601 aaaatcatcc agctcgcgcc caacaaccag cacgcctata acgcgctggg ttattcgctg
     3661 gccgatcgca acattcgcct gccggaag
//
LOCUS       DASRDH010000190         5679 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_12574,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000190 DASRDH010000000
VERSION     DASRDH010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 5679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5679
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12574"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..1507)
                     /locus_tag="VFA14_04355"
     CDS             complement(83..1507)
                     /locus_tag="VFA14_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007879958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HZS80642.1"
                     /translation="MKQLKTSRMLLLAPLLMSLAGCTSGPGYIRPGMGTPAAFKEAQG
                     WRPAKPVELNMNGKWWEMFSDAQLDRLVEQIDISNQNLAIAEANFRKAMALVRSARAA
                     AYPTMTLDVSKLRSTTAGSAALKDSTTMSLDAGWEIDVWGRVRRNVESSEASAQATAA
                     DLAAARLSAQAQLAQNYLQLRVTDARQQLLDDTVSAYQRSYQMTQNQYAVGVVARSDV
                     IQAQAQLKSAQAQALDNGVQRAQLEHAIALLAGQPASGFSIQPSPLTATAPPVPLALP
                     SMLLERRPDIAAAERRAAAANAQIGVAQSAYFPDLSLSASGGYQTTSGIVNLFRLPSR
                     IWSLGPQLGQLIFDGGASKAATSQAIAAYDAAVASYRQTVLTAFQEVEDNLAALRILE
                     REAALQDQALQAARQAVTLVLNQYKAGTVSYVSVVTAQATALAGEIAALDILNRRMAA
                     SVLLAKALGGDWSERNIADQQETD"
     gene            1688..2908
                     /locus_tag="VFA14_04360"
     CDS             1688..2908
                     /locus_tag="VFA14_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007879507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HZS80643.1"
                     /translation="MRESSRPPAYAGDIGIVGNGVIGKTAALALAQAGLRVNLLMPHI
                     ASTPAGEQDGWDVRVYAVNQIARDLLSSVKVWDALDAERVAAVEGMEVTGDAGHHPGR
                     LSFDAYAAHVDALAWIVEDRHLNTALDAALKFASNVHMVNGRAVSLVADAQGVDLQLD
                     NGDILGASLLIGADGAQSWVRGQCDIGLDYRSYGQHAIVTNFACAKAHQGVAYQWFTA
                     EEGIIALLPLPGQRVSLVWSAPHALAETLLRQPAAQLAQHLEKYAGHTLGALIPLAPE
                     QVRGFPLTLVKPHAMIAARVALIGDAAHVIHPLAGHGMNLGFADVVALLKVIGEREQW
                     RDCGDARVLSRYARARKEDVLLMQLATDGLARLFESSAEPVRFVRNAGLNLVNHLPVL
                     KRRLMAHALGKQAC"
     gene            2958..3680
                     /locus_tag="VFA14_04365"
     CDS             2958..3680
                     /locus_tag="VFA14_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011979456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HZS80644.1"
                     /translation="MITLRAAAAGSLLAYAGATAYAAAATEATIKKLIGPRLGAGVKV
                     ESVTRTPYAGLYEVQVDGDVIYTDAKAQYLFIGRVVDAQTHRDFTKEKIQAINKVNFA
                     DLPLELAIKTVKGNGKRIIAVFEDPNCGYCKRFQKTLQSVDNVTVYTFQYNILAQDSI
                     EKSRNIWCASSPDKAWSNWMISDREAPAAPAACSAPHEKVLALGQKMRVTGTPTIIFA
                     DGSRIAGAIDAKTLEQKFSTLK"
     gene            complement(3735..>5679)
                     /locus_tag="VFA14_04370"
     CDS             complement(3735..>5679)
                     /locus_tag="VFA14_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017454136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZS80645.1"
                     /translation="LGAREVSFTVLSMSISLIAVFIPILMMGGIIGRLFREFAVTLSV
                     AILVSLLVSLTTTPMMCARLLRRENQAVRQPGRFFSAIEVWFAVMLRGYERSLAWALR
                     FSPLMMLILLATIALNFYLYVIVPKGFFPQQDTGRLIGSIRGDQSISFQAMRVKLDEF
                     IDIVRQDPAVENVTAYTGGGQRNRGNMFIVLKPLSERRISANEVIARLRGKLAHVVGA
                     RLILQAAQDIRIGGRQSDAQYQYTLQSDDLELLRSWEPKIRQAFSSLPQLADVNTDQQ
                     DKGLQTSLTIDRETAMRSGVTPQLIDSSLNDLFGQRQISTIYSGMNQYHVVMEAAPQY
                     WQSPEILNDTYVSLSGSSANLSPTTSALGTPPALTAGGRQNASASLALALTLSTAVAT
                     QVPLMSFSHFAPTTTSLSVNHQGQFIASTISFNLAAGVSLSQATAVIDDALARLGVPT
                     AVHGAFAGTANAFQSSLGSQPVLILTALLAVYIVLGVLYESLVHPITIISTLPSAGVG
                     ALLALLVTGTDFSLIALIGVMLLIGIVKKNAIMMIDFALQAERMRGLSPREAIFEACC
                     LRFRPIMMTTMAALLGAVPLALGSGDGAELRRPLGISIVGGLAVSQMLTLYTTPVVYL
                     YMERFRLWSREVFEHKKNRTEMK"
BASE COUNT         1158 a         1735 c         1696 g         1090 t
ORIGIN      
        1 cgacgcgcaa gacgtggtga tctgcaacgc cctgcgcggc gtgatgcgcg cagatgtcgt
       61 ccagggtgta ccggcataag gttcagtcgg tttcctgctg gtcggcaata ttgcgctcgc
      121 tccagtcacc acccaacgcc ttggccagca acacgctggc agccatacgg cggttcagga
      181 tatccagcgc cgcgatctcg cccgccagcg ccgtcgcctg cgcggtgacg acgctgacat
      241 agctgacagt gccagctttg tactggttga gaaccagcgt tactgcctgg cgcgccgcct
      301 gcagggcttg gtcctgcaac gcggcttccc gctcgaggat gcgcaaggct gccagattat
      361 cttccacttc ctggaaagcc gtcagcaccg tctgcctgta gctggccacc gctgcatcat
      421 aggccgcaat cgcctgactg gtcgcggcct tgctggcgcc gccgtcgaag atcagctgac
      481 cgagttgcgg tcccagcgac cagatgcggc tcggcaacct gaacagattg acgatgccgc
      541 tggtggtctg atagccgcct gacgccgaca gcgacaggtc cggaaaatac gccgattgcg
      601 ccacgccgat ctgggcattc gcggcagcgg cgcggcgctc ggcggcggca atgtcaggac
      661 ggcgctccag cagcatcgac ggcaaggcca gcggaaccgg cggcgccgtt gccgtcagcg
      721 gcgacggctg aatcgagaaa ccggatgcgg gttgccctgc cagcagcgcg atcgcatgct
      781 ccagttgtgc gcgctgcaca ccattgtcga gcgcctgcgc ctgcgcgctc ttcagttgcg
      841 cctgcgcctg gatcacatcg gacctggcga ccacaccgac agcatactgg ttttgcgtca
      901 tttgataaga gcgctgataa gcgctcacgg tgtcgtccag caactgctgc cgggcatcgg
      961 tcacacgcag ttgcaaatag ttttgcgcca gttgcgcctg cgcgctcaga cgcgcggccg
     1021 ccagatcagc agcggtcgcc tgggcgctcg cctcactgga ctccacattg cggcgcacgc
     1081 gcccccaaac gtcgatttcc caaccggcat cgagcgacat cgtggtgcta tccttgagcg
     1141 ccgcactgcc tgccgtggtg gagcgcaact tgctgacatc cagcgtcatc gtcggatagg
     1201 cagccgcgcg cgcgctccgc accaacgcca tggccttgcg aaaattagcc tcggcaatgg
     1261 caagattctg gttggagata tcaatctgct ccaccagcct gtcgagctgg gcatcgctga
     1321 acatttccca ccacttgccg ttcatgttca gttcgactgg cttggccggc ctccagccct
     1381 gcgcttcctt gaaagctgcc ggcgtgccca taccggggcg gatgtagccg ggaccggacg
     1441 tgcatcccgc cagagacatc agcagcggcg ccagcaacag catgcgactg gttttcagtt
     1501 gcttcatccc gatttctcca ctggtatcgc atgcgcatgc tgcgtccgga tgcatttgct
     1561 gattcgttgg caaattatat tgtagtggtc tgatttacgc agacacctca agaaaaagac
     1621 tggcgcgaag ccgcttataa tgtcccccat gaacggtcaa acaccacatt cctgtctggg
     1681 cgcgtccatg cgtgaatctt cgcggccgcc ggcatatgcc ggcgatattg gtatcgtcgg
     1741 caatggcgtg atcggtaaaa ccgcggcatt ggctctggcg caggccgggc tgcgcgtgaa
     1801 tctgctgatg ccgcacatcg cttcgacgcc ggcaggtgag caggacggtt gggacgtgcg
     1861 cgtgtacgcc gtcaatcaga tcgcccgtga tttactgtcg tcggtcaagg tgtgggatgc
     1921 gctcgatgca gaacgcgttg ccgctgtcga aggtatggaa gtcaccggcg atgccgggca
     1981 tcatccgggc cgtttgtctt ttgatgctta cgccgcgcac gtcgatgcgc tcgcctggat
     2041 cgtggaagat cgccatctga atacggcgct cgatgcagcg ctgaagtttg cgtcaaatgt
     2101 gcatatggtc aatggccgtg ccgtatcact cgtagctgac gcgcaaggcg tcgatctgca
     2161 actcgataac ggcgacatac tgggagcatc tctactgatc ggcgccgatg gcgctcagtc
     2221 gtgggtacgc gggcaatgcg atatcggtct ggattatcgc tcttacggtc agcatgccat
     2281 cgtcaccaat tttgcctgcg caaaagcgca tcagggagtg gcctatcaat ggttcacggc
     2341 ggaggaaggt attatcgcct tgttgccgtt gccgggacag cgcgtgtcgc tggtgtggtc
     2401 ggcgccgcat gcgctggcgg aaactttgct gcgtcagccg gctgcacaac tggcgcagca
     2461 tctggagaaa tatgcgggcc acacgctggg cgcattgata ccgttggcgc cggagcaggt
     2521 gcgcggtttc ccgctgacgc tggtcaagcc gcatgccatg atagccgcgc gtgtcgcgct
     2581 catcggcgac gccgcgcatg tgatccatcc gctggccgga cacggcatga atctcggttt
     2641 cgccgatgta gtggctttgc tgaaggtgat cggcgaacgt gagcaatggc gcgattgcgg
     2701 cgatgcgcgg gtgctgagtc gctacgctcg cgcccgcaag gaagacgtgc tgttgatgca
     2761 actggccact gatggtctcg cgcgtctgtt tgaaagcagt gccgagcctg tacgtttcgt
     2821 gcgtaatgcc ggattgaacc tggtcaacca tctgcctgtc ttgaagcgac gtctgatggc
     2881 ccatgcgctt ggtaaacagg cttgctgatc atggccaacg gcaacgctta tcttcccatg
     2941 gaatccatat gaacaaaatg atcacactgc gcgccgccgc tgccggttcc ttgctggctt
     3001 acgcgggtgc tacggcatat gctgccgccg ccactgaagc caccatcaag aaattgatcg
     3061 gtccgcgcct cggcgcgggc gtcaaggtcg agtcggtgac caggacgccc tatgcaggtc
     3121 tgtatgaagt acaggtagac ggtgatgtga tttataccga cgccaaggcg caatatctgt
     3181 tcatcggccg tgtggtcgat gcgcaaaccc atcgtgactt taccaaggaa aaaatccagg
     3241 cgatcaacaa ggtgaacttc gccgatctgc cgctggaatt ggcgatcaag acagtcaagg
     3301 gcaacggcaa acgcatcatc gcggtgttcg aggatcccaa ctgcggctat tgcaagcgtt
     3361 tccagaagac cctgcaaagc gtcgacaacg taaccgtgta taccttccag tacaacatcc
     3421 tggcgcagga ttccatcgag aagtcgcgca atatctggtg cgcctccagt cccgacaagg
     3481 cctggagtaa ctggatgatc agcgacaggg aagcgccggc cgcaccggct gcgtgcagcg
     3541 cgccacatga gaaagtactg gcgctgggtc agaaaatgag agtgaccggc acgccgacca
     3601 tcattttcgc tgacggctca cggattgcgg gcgccatcga cgcgaagacg ctggaacaga
     3661 aattctctac gctgaagtaa gaagctgttg acgatctcct gcggggccgt ctggtcgccc
     3721 tttcaggaaa aactctactt catttcagtc ctgtttttct tgtgttcgaa cacttccctg
     3781 ctccacaggc ggaagcgctc catatacaga tacactaccg gcgtcgtgta cagggtcagc
     3841 atctggctga ccgccagacc gccgacgatc gatatgccca gcggtcggcg cagctcagca
     3901 ccatcgccgc tgcccagcgc cagcggcacg gcacccagca aggccgccat ggtggtcatc
     3961 atgatggggc gaaaacgcaa acagcaggcc tcgaaaattg cctcgcgcgg cgacaggccg
     4021 cgcatgcgtt ccgcctgcaa ggcgaagtcg atcatcatga tcgcattttt cttgacgatg
     4081 ccaatcagca acatcacgcc gatcagcgcg atcaggctga agtcggtgcc agtgaccagc
     4141 aaggccagca aggcgccgac gccagccgag ggcaaggtcg agatgatggt gatcggatgc
     4201 accaggcttt catacaacac gccgagtacg atatacaccg ccagcaaagc cgtcaggatc
     4261 aacaccggct ggctgcccag cgacgactgg aaggcattcg ccgtgccggc aaaagcgcca
     4321 tgcaccgctg tcggcacgcc cagccgcgcc agtgcatcgt cgatgacagc cgtggcctgc
     4381 gacagcgata cgccggcggc cagattgaaa gaaatcgtag aagcgatgaa ttgtccctga
     4441 tggttcacac tcagcgaggt cgtggtcggc gcaaaatggg agaacgacat caacggcacc
     4501 tgcgtcgcaa ccgccgtcga cagcgtcagc gccagcgcca gcgaggcgct ggcattctgc
     4561 cggccgcccg ccgtcagcgc tggcggcgtg cccagcgccg aggtcgtcgg cgacagattg
     4621 gcactgctac cgctcaagct gacataggta tcgttgagga tttccggact ttgccagtac
     4681 tgcggcgcgg cttccatgac gacgtgatac tggttcatgc cgctgtagat ggtggaaatc
     4741 tgccgctggc cgaacaagtc gttcaggctg gaatcgatca gttgcggtgt cacgccgctg
     4801 cgcatcgccg tctcgcgatc gatcgtcagt gaagtctgca aacccttatc ctgctgatcg
     4861 gtattgacgt cggccagttg cggcaggctg gagaaggcct gccggatctt cggctcccag
     4921 ctgcgcaaca gttccagatc atccgattgc agcgtgtact gatattgcgc atcgctctgg
     4981 cgcccgccga tgcgaatatc ctgcgccgcc tgcagaatca ggcgggcgcc gaccacatgg
     5041 gccagcttgc cgcgcaggcg ggcgatcacc tcgttggcgc ttatcctgcg ctccgacaaa
     5101 ggcttgagta caataaacat attgccgcgg ttacgctggc cgccgccggt ataggcggtg
     5161 acgttttcca ccgccggatc ctgacgcacg atgtcgatga actcatccag cttgacgcgc
     5221 atcgcctgga aggaaatcga ctggtcacca cgtatactgc caatcagccg tccggtatcc
     5281 tgctgcggga aaaaaccctt gggcacgatc acgtacagat aaaaattgag cgcgatggtc
     5341 gccagcaaga tcagcatcat cagcggcgaa aaccgcaacg cccaggccag cgaacgttcg
     5401 tagccgcgca acatcacggc aaaccagact tcgatagccg agaaaaaacg tcccggctgg
     5461 cgtaccgcct ggttctcgcg cctgagcagc ctggcgcaca tcatcggcgt agtggtcaaa
     5521 gacaccagca gcgacaccag aatcgccacc gacaaagtga cggcaaattc acggaacaag
     5581 cgcccgataa taccgcccat catcaggatg ggaataaaaa cggcgatcag cgaaatactc
     5641 atcgacagca ccgtgaaact gacttcacgc gcgccgagc
//
LOCUS       DASRDH010000191         2258 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_589624,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000191 DASRDH010000000
VERSION     DASRDH010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_589624"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(348..1430)
                     /gene="queG"
                     /locus_tag="VFA14_04375"
     CDS             complement(348..1430)
                     /gene="queG"
                     /locus_tag="VFA14_04375"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HZS80646.1"
                     /translation="MRSSLSILAGTIKAWGRELGFADLRIADADLSDREAGLQAWLDK
                     GYHGEMDYMASHGMKRARPAELVPGTARVITVYMPYLPRPASQSDADWRAREAARADG
                     AAVISLYARGRDYHKVLRARLQQLASLIEKEVGSFGYRVFTDSAPVMEVALAEKSGLG
                     WRGKHTLLLNREAGSMFFLGELFTDLALPVDAPITPHCGRCRACIDVCPTQAIVAPYE
                     LDARRCISYLTIELKESIPVALRSLIGNRVYGCDDCQLHCPWNKFAQISDLPDFDVRH
                     GLDHASLVSLFNWSADDFRRRTEGSAIRRIGHERWLRNMAVGLGNTAVTRKGDPDIVA
                     ALQARLTHFSELVREHTLWALEQHFL"
     gene            1471..1956
                     /gene="tsaE"
                     /locus_tag="VFA14_04380"
     CDS             1471..1956
                     /gene="tsaE"
                     /locus_tag="VFA14_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006462917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HZS80647.1"
                     /translation="MSPYHTHLQDEARTAALGAALARVLTPGLAIYLYGNLGSGKTAL
                     TRALLHAAGYAGCIKSPTYTLAEPYTVTLADRMVPVIHFDLYRMAGPEEFLEAGFREH
                     FNENNICIIEWPERGESVLPTPDIHISLTVAGQGRDVKLLALSDKGHQCLARLKFAPN
                     P"
     gene            1920..>2258
                     /locus_tag="VFA14_04385"
     CDS             1920..>2258
                     /locus_tag="VFA14_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMIN domain-containing protein"
                     /protein_id="HZS80648.1"
                     /translation="MPRSTEIRPKSVTFRTRRTTLKASGALLISVLTPLPALAAQILA
                     VRVWPSDDYTRVTLENDSNLKVSHFIVKNPERLVVDIEGIDLNSTLKNLVAKIQPNDP
                     YIKQVRVGQNR"
BASE COUNT          550 a          663 c          578 g          467 t
ORIGIN      
        1 ggccaacgat gccggcgatg gaaagcacca gccgcgctgg cttggaaaca ggctcaatat
       61 cagtagcgtt ttgcataaaa ttttcctatc gatgacagaa tagatatctc caagttacag
      121 gaacacatct ctgtgtcttt gctacatcaa tcacgcgtta cgctgcccta cgacataatc
      181 ccaatatgtc ttcttataca cgatagtatc ttgcgcaaaa aaaaaatgtc aatttttaaa
      241 atagaaaaaa tagaaaatta gttcaaaatc cacaacttga cgaaattcat ctaaaaattt
      301 aacgtaaaca ccacattcac tgctccagtg aaagcaagag tacccgttta caggaaatgc
      361 tgctccaacg cccaaagagt gtgttcgcgc accagttcgg aaaaatgcgt cagccgcgcc
      421 tgcaatgcag cgacaatgtc cggatctccc ttgcgcgtaa cagcggtatt acctagacca
      481 acggccatat tgcgcaacca gcgctcatgg ccaatgcggc ggatcgcgct accttcggtg
      541 cgacgcctga aatcgtctgc gctccaattg aacagactca ccagcgaagc atggtcgagg
      601 ccatgacgca catcgaagtc cggtaaatcg gaaatctgcg cgaacttgtt ccacgggcaa
      661 tgcagctggc aatcgtcaca gccatagacg cggttaccga tcaggctgcg caacgcaacc
      721 ggaatgcttt ccttcaattc gatggtgaga taggaaatac agcgacgcgc gtcgagttcg
      781 taaggcgcga cgatggcctg ggtcggacaa acgtcgatac aagcgcggca ccggccgcag
      841 tgcggggtga tcggtgcgtc gaccggcaac gccagatcgg tgaacaattc gccgaggaag
      901 aacatggaac cggcttcacg gttgagcaac agcgtgtgct tgccgcgcca accgaggccg
      961 gatttttccg ccagcgcaac ttccatcact ggagcggaat cggtaaagac gcggtaaccg
     1021 aaactgccga cttctttttc gatgaggctg gcgagctgct gcaatctggc acgcaagacc
     1081 ttgtggtagt cgcgtccgcg cgcatacagc gagatcacgg cagcgccgtc agcgcgcgct
     1141 gcttcgcgcg cgcgccagtc ggcgtcgctt tggctggctg ggcgcggcag ataaggcatg
     1201 tacaccgtga tcacgcgtgc cgtacctggc accagttcgg caggccgggc acgcttcatg
     1261 ccgtggcttg ccatataatc catttcaccg tggtaaccct tgtccagcca tgcctgcaac
     1321 ccggcttccc gatcagagag atcggcatcg gcgatgcgca agtcggcgaa cccgagttcg
     1381 cgtccccagg ctttgatggt gccggcaagt atgctcaggc tggaacgcat tttatccaaa
     1441 atttttgcag cctcaactac ggtttttttt atgtcgcctt accataccca cttgcaggac
     1501 gaagcccgca ccgctgcgct gggcgccgcg ctggcgcggg tactcacgcc aggtctggca
     1561 atctatctgt acggcaatct cggctccggt aaaaccgcct tgacgcgcgc gctgctgcat
     1621 gccgccggtt acgctggatg catcaaaagt ccgacttata ccctagccga accttataca
     1681 gtgacactcg cagaccgcat ggtacctgtt atccacttcg atctctaccg tatggccggc
     1741 ccggaagaat ttctggaggc cggattccgt gaacatttca acgaaaacaa catctgcatc
     1801 attgaatggc cggaacgggg cgagtcagtg ttgccaaccc ccgatatcca tatctcgcta
     1861 acggttgccg gacagggacg tgatgtaaaa ttactggcat tgtctgacaa gggtcatcaa
     1921 tgcctcgctc gactgaaatt cgccccaaat ccgtgacatt caggacacgc cgcactaccc
     1981 tcaaggcaag tggcgcgctc ctgatctccg tattgacgcc cctgccggca ctggcggcgc
     2041 agatcctcgc cgtgcgcgta tggccatccg acgactatac ccgcgtcacc ctggaaaacg
     2101 acagcaatct caaggtcagc catttcatcg tcaagaatcc ggagcgcctg gtcgtcgaca
     2161 tcgaaggcat tgacctgaat tcgacgctga aaaatttagt cgccaagatc caacccaacg
     2221 atccctatat caaacaagtc cgtgtcggcc agaatcgc
//
LOCUS       DASRDH010000192         3648 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_13146,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000192 DASRDH010000000
VERSION     DASRDH010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 3648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3648
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13146"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..132
                     /locus_tag="VFA14_04390"
     CDS             <1..132
                     /locus_tag="VFA14_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HZS80649.1"
                     /translation="SVAVIAGVSLPMLLRALTYRNDTLDVVVAMALTGARNGAVRVV"
     gene            179..448
                     /locus_tag="VFA14_04395"
     CDS             179..448
                     /locus_tag="VFA14_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010101417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HZS80650.1"
                     /translation="MIRKELEIINKLGLHARASARFTQLASRYKCDVWMTFNGRRVNA
                     KSIMGVMMLAAGKGSMVLLETEGEDEQACFDALEALVQDCFGEGQ"
     gene            481..2256
                     /gene="ptsP"
                     /locus_tag="VFA14_04400"
     CDS             481..2256
                     /gene="ptsP"
                     /locus_tag="VFA14_04400"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HZS80651.1"
                     /translation="MASFVLHGIPVSRGIAIGRAHLLAPAALDVRHYLIPEDQVEAEV
                     QRLKNAIAVVHQELQVIRHELPKEAPPELDALIGVHVMILSDPMLADMPFNIIRHRYY
                     NAEWALVTQIDGLSAQFDEIEDAYLRERKADIQQVGDRVLKVLTGSAVDLPPAAGGDD
                     ATASIIVVAHDISPADMLQFRDGAFGGFVTDRGGQNSHAAIVARSLDIPAVVGMNNAS
                     ALVKQDDVLIIDGDAGIAIIDPSPLVLTQYRTRQERMLRDRKKLGRLKKTPAITQDGT
                     GIMLLANIELPTDSGPALEAGANGIGLFRSEFLFMGRAGHPEKFPAEEEQLTSYRQAV
                     EAMKGRPVTIRTLDVGADKPLDENGQTALNPALGLRAIRYCLSEPQMFLTQLRAILRA
                     SAFGPVKLLIPMMAHAFEVDQTLALIEQAKGQLRQRQQKFDEHIEIGAMIEIPAAALA
                     LPMFIKRLDFLSIGTNDLIQYTLAIDRVDHEVAHLYDPLHPAVLFLLSNVIAMGRKAD
                     IPVAVCGEMAGDVKWTRLLLGMGLLEFSMHPSQLLSVKQEILGSDLTLLEPQVKKVLR
                     ATEPSVIEAAMAQLRVLHAGPETRA"
     gene            2320..3459
                     /locus_tag="VFA14_04405"
     CDS             2320..3459
                     /locus_tag="VFA14_04405"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HZS80652.1"
                     /translation="MSIGIVSPRIMHFQEALPLKNGTALSGYDLVYETYGTLNVDKSN
                     AVLVCHALNASHHVAGIYEDDPGNVGWWNNMVGPGKPLDTDRFFVIGINNLGSCFGST
                     GPMHTNPATGKTYGASFPVVTVEDWVNAQARLADRIGITQFAAVMGGSLGGMQALAWS
                     MMFPDRLRHCIVIASTPKLSAQNIAFNDVARQAILADPDFHGGDFYTHGVVPKNGLRV
                     ARMVGHITYLSDDDMAERFGRDLRSGEYQFGFGIDFEIESYLRYQGDKFSAYFDANTY
                     LLITKALDYFDPAKDFGGDLVKAFAGVRAQFLLASFTTDWRFSPECSREMAQALVSNR
                     RRVVYAEIDAPHGHDAFLLEDARYMKLLREYYRRVWAEITVEACA"
     gene            3456..>3648
                     /locus_tag="VFA14_04410"
     CDS             3456..>3648
                     /locus_tag="VFA14_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HZS80653.1"
                     /translation="MNFPELSRLRPDLAFIAHWVRAGTQVLDVGCGDGVMLDYLQTDK
                     ACSGYGIEIDDARIPECVAR"
BASE COUNT          752 a          992 c         1102 g          802 t
ORIGIN      
        1 agcgttgcag tcattgcagg tgtcagtttg ccgatgttgc tgcgcgcact gacgtatcgc
       61 aacgatacac tcgatgtcgt cgtggcaatg gcactgactg gtgcccggaa cggcgccgtg
      121 cgcgtggtat aagcagattc gtgttttttc ttgttgtcac aaaaaagaaa gagttttcat
      181 gattcggaaa gaacttgaga tcatcaacaa gctcggcttg catgcgcgcg cctcggccag
      241 attcacccag ctggcctcca gatacaagtg tgatgtctgg atgactttca acggacgccg
      301 tgtcaacgcg aaatccatca tgggcgtcat gatgctagcc gcaggcaagg gcagcatggt
      361 tttgttggaa accgaaggcg aggatgagca ggcgtgtttc gatgcgctgg aagcattggt
      421 gcaggactgt ttcggcgaag ggcagtaaga acgcgttgac gaatgcggga gagaagcgcg
      481 atggcatctt tcgttttaca cggcatcccg gtttccagag gcatagccat aggccgcgcg
      541 catctgctcg cccctgcggc actggatgtc agacattacc tgatccccga ggatcaggtc
      601 gaggcggaag tgcaacgtct caagaacgcg attgccgtcg tccatcaaga gctgcaggtg
      661 atacgtcatg agctgccgaa ggaggcgccg cctgaattgg atgcgctcat cggtgtgcat
      721 gtgatgattc tgtctgaccc gatgcttgcc gacatgccgt tcaatatcat ccgtcatcgc
      781 tattacaatg ccgaatgggc attggtgacg cagatcgatg gcttgtcggc gcagttcgat
      841 gaaatcgagg acgcttatct gcgcgaacgc aaggctgaca ttcagcaagt cggcgaccgc
      901 gtactcaagg tactcaccgg cagcgcggtc gatttgccgc cagccgcagg cggcgatgac
      961 gcaaccgcca gtatcattgt cgtggcacac gacatttctc ctgccgacat gctgcaattc
     1021 cgggacggcg ctttcggcgg tttcgtcacg gaccgcggcg gccagaattc gcacgcggca
     1081 atcgtggccc gcagtctcga catccccgct gtcgtcggga tgaacaatgc ttcggcgctg
     1141 gtcaagcagg acgatgtgct aatcatcgat ggcgatgccg gtatcgccat catcgatccg
     1201 tcgccgttgg tactgacaca gtaccgcacg cgccaggaac gcatgttgcg cgaccgcaaa
     1261 aagctcggtc gcctcaagaa gaccccggcg atcacgcagg acggtaccgg gatcatgctg
     1321 ctggccaata tcgaattacc gaccgacagt ggcccggcgc tggaagcagg cgccaacggc
     1381 atcggcctgt tccgttccga attcctgttc atggggcgtg ccggtcatcc ggaaaaattt
     1441 ccagcggagg aagaacagtt gacgtcgtat cgtcaggcag tggaggctat gaaggggcgc
     1501 ccggtgacga tccgcacgct tgacgtcggc gctgacaaac cactcgacga aaatggacag
     1561 actgccttga acccggcgtt gggtttgcgc gcgatccgct actgcctgag tgagccgcag
     1621 atgtttttga cacagttgcg tgcgattctg cgcgcttcgg cttttggtcc ggtcaaactg
     1681 ctgattccga tgatggcgca tgcgtttgaa gtcgaccaga cgctagcgct gatcgagcag
     1741 gccaaggggc agttgcgtca gcgtcagcag aaattcgatg aacacatcga gatcggcgct
     1801 atgatcgaaa ttccggctgc cgcgctggcc ttgccgatgt tcatcaaacg tctcgatttt
     1861 ctatcgatcg gcacgaatga cctgatccag tacacgttgg caatcgatcg cgtcgatcat
     1921 gaggtggcgc atctgtacga tcccttgcat ccggcggtgc tgtttctatt gtcgaacgtg
     1981 attgcgatgg ggcgcaaggc cgatatcccg gtggccgtgt gcggtgaaat ggccggtgac
     2041 gtcaagtgga cacgcctgct gctgggtatg ggcttgctgg agttttccat gcacccttcg
     2101 cagttgctgt cggtcaagca agaaatcctg ggcagtgacc tcactttgct ggaaccacag
     2161 gtcaagaagg tgctcagggc cactgaacca tcagtcatcg aagctgccat ggcgcaattg
     2221 cgcgtgctgc atgccgggcc ggagaccagg gcttgagagg caagcatatg ccgtccggca
     2281 tttgcatacg gctcaaacgc aggacaacaa agagaatcca tgtcgatcgg aatcgtatcg
     2341 ccccgcatca tgcacttcca ggaagcattg ccgctcaaaa acggcacggc gctgagcggc
     2401 tatgacctgg tgtatgaaac ctacggcacg ctcaatgtcg acaaatccaa cgctgtgctg
     2461 gtgtgtcatg cgctcaatgc ttcgcaccat gtcgccggca tctacgagga tgaccccggc
     2521 aatgtcggct ggtggaataa catggttggt cccggcaagc cgctggatac agataggttt
     2581 ttcgtcatcg gcatcaataa tctcggttcc tgttttggtt caaccggtcc gatgcatacc
     2641 aatccggcca ccggcaagac ctatggtgcg agttttccgg tcgttaccgt ggaagactgg
     2701 gtcaatgccc aggcgcgtct ggcggatcgg atcggcatca cgcaatttgc cgcagtcatg
     2761 ggtggttcgc tgggcggcat gcaggcactg gcgtggagca tgatgttccc ggatcgcctg
     2821 cgtcattgca tcgtgattgc ctcgacgcca aaactgtcgg cccagaacat cgctttcaac
     2881 gacgtggcac gccaggctat cctggccgat ccggattttc atggcggcga tttctatacc
     2941 catggcgtgg tgccgaaaaa cggcctgcgt gtagcacgca tggtggggca tatcacctat
     3001 ttgtcagatg acgacatggc tgaaagattc ggccgcgatc tgcgttccgg cgagtatcag
     3061 tttggcttcg ggatcgactt cgaaatcgaa tcttacctgc gttatcaggg cgacaaattt
     3121 tcggcctact tcgatgccaa cacctatttg ctgattacca aggcactcga ctacttcgat
     3181 ccggccaagg atttcggcgg cgatctggtc aaggcttttg ccggtgtcag ggcgcaattt
     3241 ctgctggcgt cgtttaccac cgactggcgc ttctcgcccg agtgcagccg cgaaatggcg
     3301 caggcgctag tgagtaacag gcgccgggtg gtctatgcgg aaatcgatgc gccgcacgga
     3361 catgatgctt tcctgctgga agatgcgcgt tacatgaaac tgctgcgtga atactatcgg
     3421 cgcgtgtggg cggaaattac cgtggaggcg tgcgcatgaa tttccctgag ctgagccggt
     3481 tgcgtcccga tcttgccttc atcgcgcact gggttcgcgc cggcacgcag gttctggacg
     3541 tgggttgtgg cgacggcgtg atgctcgatt atctgcaaac cgacaaagct tgcagcggct
     3601 acggcatcga gattgatgac gcgcgcattc cggaatgcgt ggcgcgta
//
LOCUS       DASRDH010000193         2085 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_939279,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000193 DASRDH010000000
VERSION     DASRDH010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_939279"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /locus_tag="VFA14_04415"
     CDS             complement(<1..578)
                     /locus_tag="VFA14_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013232385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HZS80654.1"
                     /translation="MKHRVKNIHFVGIGGSGMSGIAEVMRNLGYGVSGSDLGSNAATQ
                     RLAQLGARIFYGHAADNIDNADAIVISTAVKADNPEVLAARERHIPVVPRAVMLGELM
                     RLKQGIAIAGTHGKTTTTSLVASVLAQGGLDPTFVIGGRLTSAGANAKLGLGDFIVAE
                     ADESDASFLNLTPVIEVITNIDADHMETYNHD"
     gene            complement(575..1651)
                     /gene="murG"
                     /locus_tag="VFA14_04420"
     CDS             complement(575..1651)
                     /gene="murG"
                     /locus_tag="VFA14_04420"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HZS80655.1"
                     /translation="MSKRLLIMAAGTGGHIFPGLAIADTMRARGWQVSWLGTTHGMER
                     DLVPGHGIEMDAIVFAGLRGKGLMHMVKGMWRMLAACGACFRILARRRPDVVLGMGGY
                     VTVPGGAMARLRGVPLALMNADAALLLSNRILTPLADKVLFGFPADFGKVAGKAEVTG
                     NPVRKEIIALGEPAPRYAGHSGSLRMLVLGGSQGARVLNECVPAALATLPAQQRPLVT
                     HQSGKQHIDALRAAYVQAGVEAEVVDFIDDMPRRYADADLVICRAGAITVSELTAAGV
                     ASVLVPLVASTTSHQRDNAVWMVKSKAAIHVPQATLTPDGLAALLQTLDRSVCRKMAQ
                     AAYEQGRRDAGEAIAQVLERLVRQ"
     gene            complement(1651..>2085)
                     /locus_tag="VFA14_04425"
     CDS             complement(1651..>2085)
                     /locus_tag="VFA14_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006713154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HZS80656.1"
                     /translation="HSLIAFGRGELFGVGLGGSVEKLHYLPEAHTDFLLAVIGEELGF
                     SGVLVVVLLFYWLVKHAFEIGRQAIALDLTFAGLVAKGIGIWIGVQAFINMGVNLGLL
                     PTKGLTLPLMSYGGSGVLINCIGLAILLRVDYENRLLMRGGR"
BASE COUNT          471 a          720 c          522 g          372 t
ORIGIN      
        1 aaatcgtgat tgtaagtttc catgtgatcg gcatcgatgt tcgtaatgac ttcgatcacc
       61 ggcgtcaggt tcaggaaaga cgcatccgac tcatcggcct cggctacgat gaaatccccc
      121 aatcccagct tggcattcgc acccgcgctg gtcagacgac cgccgatcac gaaggtcgga
      181 tcgagtccgc cttgcgccag cacacttgcg accagactgg tggtagtggt tttgccatgc
      241 gtgccagcaa tcgcgatccc ctgcttcaga cgcatcagct cacccagcat cactgcgcgc
      301 ggcacaacgg gaatatgcct ctcgcgcgcc gccagcactt caggattgtc ggccttgacc
      361 gcagtcgaga tgacgatagc atcagcattg tcaatgttgt ccgcagcatg accatagaag
      421 atgcgcgcgc ctagctgcgc caggcgctgc gttgccgcgt tgctgcccag atccgagccg
      481 gagacaccat agcccagatt gcgcatgact tccgcaatgc cgctcattcc agagccgcca
      541 atgccgacga agtgaatgtt tttaacccga tgtttcattg ccttaccagt ctttccagta
      601 cctgcgcgat cgcctcaccg gcgtcgcgcc tgccttgctc atacgccgcc tgcgccatct
      661 tccggcagac agaccgatcc aatgtctgta acagcgccgc cagaccatcc ggcgtcaacg
      721 tggcctgcgg cacatgtatg gctgccttgc ttttcaccat ccatacagcg ttgtcgcgct
      781 gatgcgaggt agtcgatgca accaacggca ccaacacact ggccacaccc gccgctgtca
      841 attccgatac ggtgattgcg ccagcgcggc aaatcaccag atcagcatcg gcataacggc
      901 gcggcatgtc gtcgatgaaa tccaccacct ctgcctcgac cccggcctgg acatacgccg
      961 cgcgcaacgc gtcgatatgc tgcttgcccg actgatgcgt caccagcgga cgctgctgcg
     1021 ccggcaaggt cgccagcgcg gccggcacac actcattcaa tactctggcg ccctgactgc
     1081 cgcccagcac cagcatccgc agcgaaccac tatgaccggc atagcgcggc gccggttcgc
     1141 ccagcgcaat gatttccttg cgtaccggat tgccggtcac ttccgccttg ccagcgacct
     1201 tgccgaaatc ggcaggaaaa ccgaacagca ccttgtcggc cagcggcgta agtatcctgt
     1261 tcgacaataa caaagctgca tctgcattca tcagcgccag cggcacaccg cgcagacgcg
     1321 ccatcgcacc gcccggcacc gtcacatagc cacccatgcc caacaccacg tcaggccggc
     1381 gcctggcgag aatacgaaag cacgcgccgc aagctgcaag catgcgccac atgcccttga
     1441 ccatatgcat cagtcccttg ccgcgcaagc cggcgaacac gatggcatcc atctcgatac
     1501 catgtccagg caccagatcg cgttccatac cgtgcgtcgt gcccagccag gaaacctgcc
     1561 agccgcgcgc acgcatggtg tcagcaatcg ccagacctgg gaaaatatgt ccaccggtgc
     1621 cagccgccat gatcagaaga cgcttgctca tcaacgccct ccacgcatca gcaagcggtt
     1681 ttcataatcg acacgcaaca ggatcgccag tccgatacag ttgatcagca cacctgaacc
     1741 gccgtaactc atcagcggca aggtcaatcc cttggtcggc agcaaaccga gattcactcc
     1801 catgttgatg aatgcctgca caccgatcca gataccgatg cccttggcaa ccagaccggc
     1861 aaaagtgaga tccagtgcaa tcgcctgacg gccgatttca aacgcatgtt tgaccagcca
     1921 atagaacagc aagactacca ccagcacacc ggaaaaaccc aattcctcgc caatcaccgc
     1981 cagcagaaag tcggtatgcg cttctggcag atagtgcagc ttctccacgc tgccgcccag
     2041 gccgacgccg aatagctcac cgcggccgaa agcaatcagc gaatg
//
LOCUS       DASRDH010000194         2232 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1608549,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000194 DASRDH010000000
VERSION     DASRDH010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1608549"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1592
                     /gene="infB"
                     /locus_tag="VFA14_04430"
     CDS             <1..1592
                     /gene="infB"
                     /locus_tag="VFA14_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HZS80657.1"
                     /translation="GHRAHAAKLDDPEALLAEGVVHVDVESLPRAPVVTVMGHVDHGK
                     TSLLDYIRRTKVVSGEAGGITQHIGAYHVETPRGMITFLDTPGHEAFTAMRARGAKAT
                     DIVILVVAADDGVMPQTREAIAHAKAGGVPLVVAINKIDKPGANLDRVKQELIAEEVV
                     PEEYGGESPFIPVSAKTGEGIDSLLENVLLQAEVLELKAPVDVPAKGLVIEAKLDKGR
                     GPVATILVQSGTLKRGDVVLAGSAYGRVRAMLDEGGKSIVEAGPSIPVEIQGLTEVPS
                     AGEEVLVMADERKAREIGLFRQGKFRDVKLARQQAAKLENMFDQMSEGEVKNLSMIIK
                     TDVQGSQEALVQSLQKLSNAEVRVQIVHAAVGGISENDVNLAVASKAIIIGFNTRADA
                     SARKLAEASGVDIRYYNIIYDAVDEVKAAVSGMLAPEKREQVLGLVEIRQVFHVSKVG
                     SIAGCYVLEGLVKRGSSARLLRNNVVLWTGEMDSLKRFKDDVKEVKSGFECGLSLKNY
                     NDIQEGDQLEIFEVQEIARTL"
     gene            1744..2133
                     /gene="rbfA"
                     /locus_tag="VFA14_04435"
     CDS             1744..2133
                     /gene="rbfA"
                     /locus_tag="VFA14_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HZS80658.1"
                     /translation="MAKHSKSIPGRGLRVADQIQRDLSEIIAFELKDPRVGMITITEV
                     QVTPDYTHAKIFFTTLKDDRQAIQNTLTGLSKASGFLRGELGRRLTIHTLPDLHFVHD
                     DSTVRGMEMSRLIDEANATRAKDADGN"
BASE COUNT          499 a          533 c          696 g          504 t
ORIGIN      
        1 tgggtcatcg tgcgcatgcc gccaagctgg acgatcctga agccttgctg gcagagggcg
       61 tagtgcatgt cgatgttgaa tccttgccgc gtgcgcccgt cgttactgtc atgggtcacg
      121 ttgaccatgg caagacctcg ctgctggact atattcgtcg caccaaggtt gtttccggcg
      181 aggctggcgg cattacccag catatcggtg cgtaccatgt ggaaacgccg cgcggcatga
      241 tcacttttct cgacacgccg ggtcacgaag cgtttaccgc catgcgtgcc cgcggcgcga
      301 aggcgactga tatcgtcatt ctggtggtgg ccgctgatga cggcgtgatg ccgcaaacca
      361 gggaagcgat tgctcatgcg aaggccggtg gtgtgccgtt ggtggtggcg atcaacaaaa
      421 tcgacaaacc gggtgccaat ttggaccgtg tcaagcaaga gttgatcgcg gaagaagtcg
      481 tgccggaaga atacggcggc gaatcaccgt tcatcccggt gtcggcgaag actggtgaag
      541 gcatcgattc gctgctggaa aatgtcctgc tgcaagccga agtactggag ttgaaggcgc
      601 cggtcgatgt gccagccaag gggctggtca ttgaggccaa gctcgacaag ggccgcggtc
      661 cagtggcgac gattctggtg cagtccggta ccttgaagcg tggcgatgtc gtgctggcag
      721 gttcggcgta tggccgtgtg cgtgcgatgc ttgatgaagg cggcaagagc attgttgaag
      781 ccggcccttc catccctgtc gaaattcagg gactgaccga agtgccgagt gccggcgaag
      841 aagtgctggt gatggctgat gagcgtaagg cgcgtgaaat cggcctgttc cgtcagggca
      901 agttccgcga tgtcaagctg gccaggcaac aggctgcgaa gctggaaaac atgtttgacc
      961 agatgagcga aggtgaggtc aaaaacttgt cgatgatcat caagaccgac gtacaaggtt
     1021 cgcaggaagc gctggtacaa tcgttgcaaa agctgtccaa tgccgaagtg cgtgtacaga
     1081 tcgtgcatgc tgcggtcggg ggtatttccg aaaatgacgt caacctcgcg gtggcatcga
     1141 aggcgatcat catcggtttc aacacacgtg ccgatgcgtc ggcgcgcaag ttggctgaag
     1201 ccagcggtgt cgatattcgc tattacaaca tcatttacga tgcggtagac gaagtgaagg
     1261 cggcagtatc gggcatgctg gcgccagaga agcgcgaaca ggtactgggc ctggtcgaga
     1321 ttcgtcaggt attccatgtt agcaaggtcg gttcgatcgc cggctgttat gtgctggaag
     1381 gtctggtcaa gcgtggttct tcggcgcgcc tgttgcgtaa taatgtggtg ctgtggaccg
     1441 gtgagatgga ttcgctcaag cgtttcaaag acgatgtcaa ggaagtcaag tccggcttcg
     1501 aatgtggtct gtcattgaag aactacaacg atattcagga gggtgaccaa ctggagattt
     1561 tcgaagtgca ggaaatcgcg cgtaccttgt aattcagctg acaggtagaa gggcggacag
     1621 cagacatgcg atgcctgctg tccgcccggt tatattcagt tgcattcatt caaatacagt
     1681 tcagtgctgt ctgtgatgaa cgtctatcga cgtcgggcag gacggtgagg ttgattttta
     1741 cacatggcaa aacatagtaa atccattcca gggcgcggtc tgcgtgtggc cgatcagatt
     1801 cagcgcgatc tgtcggaaat catcgcattc gagttgaaag atccgcgtgt gggaatgatc
     1861 acgatcaccg aggtgcaagt aacgccggat tatacgcatg ccaagatttt cttcacaacg
     1921 ctgaaggatg acaggcaggc gattcagaat acgctgaccg ggctgtccaa agcatcaggt
     1981 tttctgcgcg gggaattggg tcgtcgcctg acgattcata cgctgcctga tttgcatttt
     2041 gtacatgatg actcgactgt acgcggcatg gaaatgtcac gtctgatcga cgaagcgaat
     2101 gccacgcgtg ccaaggatgc cgacggaaac tgaatttccc gtgatggcat gaagtcgtta
     2161 tcgcatcgat tgacgatttc cggatttgga tacacatgat gcatttctcg ccttcaaatc
     2221 cccgtcttcc ca
//
LOCUS       DASRDH010000195         3245 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_251258,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000195 DASRDH010000000
VERSION     DASRDH010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 3245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3245
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251258"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..812
                     /locus_tag="VFA14_04440"
     CDS             <1..812
                     /locus_tag="VFA14_04440"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HZS80659.1"
                     /translation="TNPTCPFTVNTLDEHLDMLMVCHHLDSAIAEDIAFAEARIRHET
                     IAAEDVLHDIGAISMMSSDSQAMGRVGEVIMRTWQNAHKMKTQRGALAGDSSCHDNFR
                     VKRYLAKYTINPAITHGISHVAGSLEVGKIADIVLWKPAFFGVKPSMILKGGMIAAAQ
                     MGDPNASIPTPQPVHCRMMFGAYGGGLKTSMTFVSQSAFDAGIGETLRLNKPVVPVQG
                     MRHLRKRDMIHNGATPRMEVDAETYEVRADGELLVCAPARVLPLAQRYFLF"
     gene            820..1326
                     /gene="ureE"
                     /locus_tag="VFA14_04445"
     CDS             820..1326
                     /gene="ureE"
                     /locus_tag="VFA14_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007881346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HZS80660.1"
                     /translation="MLTLNTRIEHAERIDGELLLPYDQREKSRLRATMTSGEEVAIFT
                     ARGAILRDGDLLRGDDGRVVKINAAREASYRVEADSPHRLLRCAFHLGNRHTQTQISA
                     GFLRIRKDPVLKDMLQGLGARVTEEEAAFEPESGAYGSGHHHDDHFHPLAPVPVRQKI
                     HRPGDEPG"
     gene            1351..2031
                     /locus_tag="VFA14_04450"
     CDS             1351..2031
                     /locus_tag="VFA14_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013236639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory UreF family protein"
                     /protein_id="HZS80661.1"
                     /translation="MQTQALLHLLQLASPLLPIGAYSYSQGLEAAIENGTVKHEDSAR
                     AWIVDTLHEVVARFEAPILWRLLRAFAERDANAVALWSERFIAARDTAEFRAETMQMG
                     YSLGKLAVDLQIGDDDLRGLMPAQLEAPLPTALAYAAVALNVPHDAALLSMLFAWAEN
                     QVLVCVKSIPLGQVAGQALLLALQPELEAAAQTAQALTDDELANWSPGLSLLSMEHEV
                     QYSRLYRS"
     gene            2070..2702
                     /gene="ureG"
                     /locus_tag="VFA14_04455"
     CDS             2070..2702
                     /gene="ureG"
                     /locus_tag="VFA14_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HZS80662.1"
                     /translation="MSNLSFPHPLRVGIGGPVGSGKTALCEMLCRHMRDHYEMAVITN
                     DIYTKEDMEILLRADVLPAERLMGVETGGCPHTAIREDASINLEAIARMSADFPDLDL
                     ILVESGGDNLAATFSPELSDLTIYVIDIAGGEKIPRKGGPGITRSDLLIINKTDLAPY
                     VGANLDIMARDARRMRGERPFVFTNLRSGDGVKEVIDYIRRQGLLDEKKG"
     gene            2886..>3245
                     /locus_tag="VFA14_04460"
     CDS             2886..>3245
                     /locus_tag="VFA14_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007881359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HZS80663.1"
                     /translation="MLRISPRIALCAGASMMAVLAAGSAIAHPGHPDSLMSISASFSA
                     GFSHPFFGVDHLLAMLAVGLWAAQNKHSAIWVLPLVFPSMMVVGALLACAGLSLPVAE
                     TGIAASVAVLGLLIAFAV"
BASE COUNT          638 a          923 c          975 g          709 t
ORIGIN      
        1 ccaccaaccc gacgtgtcca ttcaccgtca acacgctgga cgagcatctg gacatgctga
       61 tggtctgtca tcacctcgac tcggcgattg ccgaagatat cgccttcgcc gaggcgcgta
      121 tccgtcacga gaccattgcc gccgaagacg tcttgcatga catcggcgcg atctcgatga
      181 tgtcgtccga ctcacaggcg atgggacgtg tcggtgaagt gatcatgcgc acctggcaaa
      241 acgcgcacaa gatgaaaacc caacgcggcg cgctggccgg ggattcatcg tgtcatgaca
      301 atttccgcgt caagcgttac cttgccaagt acacgatcaa cccggcgatc acgcatggca
      361 tctcgcatgt ggccggttcg ctggaagtgg gaaaaatcgc cgatatcgtg ttgtggaaac
      421 cggcgttctt cggcgtcaag ccgtcgatga ttctgaaggg cggcatgatc gccgcagcac
      481 agatgggcga tccgaatgcg tcgattccga cgccgcagcc ggtgcattgc cgtatgatgt
      541 tcggcgcata tggcggcggc ctgaaaacct cgatgacttt cgtttcacag tcggctttcg
      601 atgccggcat cggcgagacg ctcaggctga acaagcccgt ggttccggtg cagggcatgc
      661 gtcatctgcg caagcgcgac atgatccaca acggcgcgac gccgcggatg gaagtcgatg
      721 ccgaaaccta cgaagtgcgc gccgacggcg agttgctggt gtgcgcacca gccagggttt
      781 tgccgctggc gcaacgttat ttcctgttct gaagagatga tgctgaccct caataccaga
      841 atcgaacatg ccgaacgcat agacggcgaa ttactgctgc cttacgacca gcgcgagaag
      901 agccgcctgc gcgccacgat gacatccggc gaagaagtcg ctatcttcac ggcgcgcggc
      961 gccatcttgc gcgacggcga tttgctgcgc ggcgatgatg gccgcgtggt gaaaatcaat
     1021 gccgccaggg aagcaagcta tcgcgtcgaa gcagattcgc cgcatcggtt attgcgttgc
     1081 gcctttcacc tcggcaatcg ccatacgcag acccagatca gcgcaggctt tctgcggatt
     1141 cgcaaagacc ctgtgctgaa agacatgctg caaggtctcg gcgctcgcgt tacggaagaa
     1201 gaggcagcgt ttgaaccgga atccggcgca tacggcagcg gtcatcacca tgatgaccat
     1261 tttcatccgc tggcgccggt tccggtgcgc cagaagattc atcgccccgg cgacgagccg
     1321 gggtgatgtt gcagatcgat agatgccagc atgcagacac aggcgcttct tcatctgctt
     1381 caactcgcca gcccattgtt gccgatcggc gcgtacagct attcgcaagg actggaagcg
     1441 gccattgaaa acggtacagt gaagcatgaa gacagtgccc gtgcatggat agtcgatacc
     1501 ttgcatgaag tcgtcgcccg cttcgaagcg ccgatcctgt ggcgtttgtt acgggctttt
     1561 gcggaacgtg atgcaaacgc cgtcgcatta tggagcgaac gttttatcgc ggcgcgcgat
     1621 acggccgagt ttcgcgcaga gacgatgcag atgggctact cgctgggcaa gctcgctgtc
     1681 gatctgcaaa tcggcgatga tgatctgcgc ggtttgatgc cagcgcaact ggaggcgccg
     1741 ttgccgacgg cgctggccta tgccgcagtg gcgctgaatg taccgcatga tgccgccttg
     1801 ctcagcatgc tgttcgcctg ggccgaaaat caggtactcg tttgcgtcaa atccattccg
     1861 cttggtcagg tcgcgggaca agctttgttg cttgccctgc aaccggagct ggaagccgcc
     1921 gcgcagaccg cccaggcatt gacagacgat gaattagcca actggtcgcc tggtctgtcg
     1981 ctgttatcca tggagcatga agtgcagtac agccgtttat accgttcctg atccgtgttt
     2041 cattgcctcc gttttcaatc gagatcatca tgagcaatct ttccttccct catcctttgc
     2101 gtgtcggtat cggcggcccg gtcggttccg gcaagacggc gctgtgcgaa atgctgtgca
     2161 ggcacatgcg cgaccattac gagatggccg tcatcaccaa cgatatttac accaaagaag
     2221 acatggaaat tctgctgcgc gccgatgtct tgccggcgga gcggctcatg ggcgtggaga
     2281 ccgggggttg tccgcatacg gcgattcgcg aagatgcatc gatcaatctg gaggcgatcg
     2341 cgcgcatgag cgcggacttt ccggatctgg atctgatcct ggtggagtcc ggcggcgaca
     2401 atcttgctgc gactttcagc cctgaactgt cggatctgac gatttatgtc atcgatatcg
     2461 ccggcggcga aaagatcccg cgcaagggcg gcccgggcat cacgcgctca gatctgctga
     2521 tcatcaacaa gaccgatctg gcgccgtatg ttggcgccaa tctggacatc atggcgcgag
     2581 atgccagacg catgcgtggc gaacgtccgt tcgtatttac caatctgcgc agcggcgatg
     2641 gcgtcaagga agtgatcgat tacatccgca gacagggttt gctggatgag aagaagggtt
     2701 aagagccgct aacacaacgc cgctggctgc gttgcgcctc cttgccgtac cttcgtactg
     2761 tcttcgtcgg cgcgccttgc cagcggcgtt gtgttagcgg ctctaagaat aaccaagcgc
     2821 ctggcctgat cattgtttca gcttgccagc gcattcgggc attgatttac ttgaaggaga
     2881 aaaatatgtt gcgtatttcc cccaggatag cgctgtgcgc cggcgcatcg atgatggcag
     2941 ttctggctgc cggtagcgct atcgcccatc ctggccatcc tgattccttg atgtccatat
     3001 cggccagctt cagcgccggt ttcagccatc catttttcgg cgtcgatcat ttgttggcga
     3061 tgctggccgt cggcctgtgg gctgcgcaga acaagcattc cgcgatatgg gtattgccgc
     3121 tggtatttcc atcgatgatg gtggttggcg ctttgctggc atgcgccggc ctgtcgttgc
     3181 cggtcgccga gaccggcatt gccgcttcgg tcgcggtgtt gggactgctg attgcctttg
     3241 cggtg
//
LOCUS       DASRDH010000196         3412 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_234382,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000196 DASRDH010000000
VERSION     DASRDH010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 3412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3412
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234382"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VFA14_04465"
     CDS             <1..198
                     /locus_tag="VFA14_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HZS80664.1"
                     /translation="RPFTAAERHAWHACLRAAALRFWLSRLYDFYLPRAAEMLTPHDP
                     THFERILRLRVAAATLTQLPQ"
     gene            204..974
                     /locus_tag="VFA14_04470"
     CDS             204..974
                     /locus_tag="VFA14_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BPSS1780 family membrane protein"
                     /protein_id="HZS80665.1"
                     /translation="MEKLPAKTGWFWIKEGIRLFRKQPAEMSTLFLGYMLLILTLGMI
                     PVIGQLLPLLLVPVFAMSFMQACRYIEEDKRVYPNLLLMGFRSPALRTLLVLGVLYLL
                     AAFGAVRLSALVDGGAFWNAMTGASPAPRRSGFDDSSLAIVAAAVIYTPAAMAFWFAA
                     PLIAWNGMSLGKSLFYSFFAVLRAGKAFVVYAVAWLAIGILLPAMISVIIASLINSTT
                     AVFFMLLPLSILSTIVIYCSFYSTYTTVFGKPQRDEVL"
     gene            complement(1037..1687)
                     /locus_tag="VFA14_04475"
     CDS             complement(1037..1687)
                     /locus_tag="VFA14_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HZS80666.1"
                     /translation="MYVPKHHEETDIDVLHALIRSHPLGAWVIHSNGEMHVNHIPFLL
                     DRSRGEHGILVGHVARGNDVYKLLTEPVNCVVIFQGSEGYITPSWYPSKHASGKAVPT
                     WNYAVVHVHGQPRAIEGRDWLLEHLNQLTDTHEAGQALPWKVSDAPDDYMERQISAIV
                     GIEIPIDKLVGKWKVSQNRTLPDKLGVIAGLLGRNDARSKEMAALVSKHSDLGNIS"
     gene            complement(1706..3100)
                     /gene="dalD"
                     /locus_tag="VFA14_04480"
     CDS             complement(1706..3100)
                     /gene="dalD"
                     /locus_tag="VFA14_04480"
                     /EC_number="1.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047813 - D-arabinitol 4-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0051161 - arabitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-arabinitol 4-dehydrogenase"
                     /protein_id="HZS80667.1"
                     /translation="MTIRDSVNDLVWLHIGAGSFHRAHQALYLHRLRECGNDEDRRWQ
                     LALANIRADMAPLLQALTQSKGSYTLETVTPQGLRAYETICSIRRIVPWDAQLKNLIR
                     IGADPRTRVISFTVTEGGYYLDHEHLLDAHHPDLVADIEGGRGTIYGAMTAILQARQA
                     SHAGSVTLLSCDNLRHNGERFFGGLQQFLTLRGQHALLAWVRDHVCSPSSMVDRITPR
                     PTDAVRRRVKDATGLDDPCALMAESFIQWVIEDDFKAGRPALEKVGVEMVASVLPYEE
                     AKIRILNASHSCIAWAGTLAGLGYIHEGMARADIQRMAYDYITQDVMACLTPSPLDLA
                     SYRDTTLARFGNPAIEDTNQRVAADGFSKIPGFIRPTLVNSLERGHQPRATALLPALF
                     FIFLQRWKQGSLPYVYQDGIFDPAATRVMLDAADPLRAYCADSLLWGELAGNAQLEHL
                     LREGVSSVNTWLAA"
     gene            complement(3167..>3412)
                     /locus_tag="VFA14_04485"
     CDS             complement(3167..>3412)
                     /locus_tag="VFA14_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS80668.1"
                     /translation="RTHHGAWLDGAEAAGAGTDFWRGKVMQEPPIVITHLVCDCDGVL
                     LDSENIALRVLGFTGVTHDRAGMAKELYETGAEKVFV"
BASE COUNT          723 a         1043 c          917 g          729 t
ORIGIN      
        1 cgcccattca ctgccgcaga acggcacgcc tggcacgcct gcctgcgtgc tgccgcgctg
       61 cgcttctggc tatcccgcct gtatgatttc tacctgccgc gtgctgccga aatgctgaca
      121 ccgcacgacc cgacgcattt cgaacgcatt ctgagattgc gcgtcgccgc ggcgacactg
      181 acgcaacttc ctcaatgaga ataatggaaa aactacccgc aaaaaccggc tggttctgga
      241 tcaaggaagg cattcgcctg ttccgcaaac agccagccga aatgtcgacg ctgttcctag
      301 gctacatgct cttgatactg acgctgggca tgataccagt gatcggccag ctactgccgc
      361 tgcttctggt gccggtattt gctatgtcat tcatgcaggc ctgccgctat atcgaagaag
      421 acaagcgcgt ctatcccaat ctgctgctga tgggtttccg ctcgccagcc ctgcgcacct
      481 tgctcgtcct gggcgtactg tatctgctgg cggcatttgg cgcagtcagg ctatccgcac
      541 tagtcgacgg cggcgctttc tggaacgcca tgactggcgc cagcccggcc cccagacgaa
      601 gcggattcga cgacagcagc ctagcgatcg tggcggctgc cgtcatctac accccagcag
      661 cgatggcgtt ctggtttgcc gcgccactga tcgcctggaa cggtatgtca ctgggcaaat
      721 ccctgttcta cagtttcttc gccgtgctgc gcgccggcaa ggcattcgtg gtgtatgccg
      781 tagcatggct ggcgatcggc atcctgctgc cggccatgat cagtgtcatc atcgcttcac
      841 tgatcaacag cacgacagcc gtcttcttca tgttattgcc gttatccatc ctgtcgacca
      901 tcgtcatcta ttgctctttt tactcgacat acacgacagt cttcggcaag ccgcagcgtg
      961 atgaagtctt gtaaatgcaa ggcaccaaac gcagcagcac cgcctgaaag agcgtctgga
     1021 cggcgccgga gcttgactag ctgatattac caagatcgct atgcttgctc accagcgcag
     1081 ccatttcctt cgatcgcgcg tcattgcgcc cgagtagccc agcaatgaca ccaagcttgt
     1141 ctggcaacgt ccggttctgg ctgactttcc acttgccgac aagtttatcg attgggattt
     1201 caatcccgac gatcgcgctt atctggcgtt ccatataatc gtccggcgca tcggacactt
     1261 tccatggaag cgcttgcccg gcttcgtggg tatcggtgag ctggttgagg tgctcgagca
     1321 accagtcacg gccctcgatc gcacgcggct gaccatgcac atgaacgacg gcgtagttcc
     1381 aggtggggac cgccttgccg ctggcgtgtt tactcggata ccacgatggt gtgatgtacc
     1441 cttcactacc ctgaaaaatc accacgcaat tcactggctc ggtcaataat ttatagacat
     1501 cgttaccgcg tgcaacatgt ccgaccaaaa tgccgtgttc tccgcgcgat cgatcaagca
     1561 gaaatggaat atggttgacg tgcatttctc cattggaatg aatcacccac gcacctagtg
     1621 gatgggatcg aatcaatgcg tgtagaacgt caatatctgt ttcttcatgg tgcttcggta
     1681 cgtacatatg cattcctcct agaaactacg cagccagcca ggtattcacg ctgctcacgc
     1741 cttcgcgcag caaatgctcc agctgcgcat tgccagccag ttcaccccac agcaaagaat
     1801 cagcgcaata agcgcgcaaa gggtcagcgg cgtccagcat gacgcgcgtt gccgccgggt
     1861 cgaaaatgcc gtcctggtac acataaggca gactgccctg tttccagcgt tgcaggaaaa
     1921 tgaaaaacag cgctggcaac aacgccgtcg cgcgcggctg atgaccgcgt tcgaggctat
     1981 taaccaaagt gggccggatg aaaccgggaa tcttggaaaa accatcagcc gccacccgtt
     2041 gattcgtatc ctctattgcc ggattgccga aacgcgccag cgtggtatcg cgataggatg
     2101 ccagatccag tggactcggc gtcagacagg ccatgacatc ctgcgtgatg tagtcgtatg
     2161 ccatgcgctg gatatctgcg cgcgccatgc cttcgtgaat gtaaccgagt ccggccaaag
     2221 tgccggccca ggcgatacag ctatggctgg cgttgagaat gcggatcttg gcttcttcgt
     2281 aaggcagcac cgacgccacc atttccacgc cgactttttc cagcgccgga cgtcctgcct
     2341 tgaaatcgtc ctcgatcacc cattgaatga aggactcagc catcagcgcg cacggatcgt
     2401 cgagaccggt ggcgtccttc acacgccggc gcacagcatc ggtcggacgc ggcgtgatgc
     2461 gatccaccat ggaactggga ctacagacat ggtcacgaac ccatgccagc aatgcatgct
     2521 gaccgcgcaa ggtcaggaac tgctgcagac cgccgaagaa acgttcgccg ttatgccgca
     2581 ggttgtcaca actgagcagg gtgacactac cggcatgact ggcttgccgc gcttgcagga
     2641 tcgccgtcat cgcgccatag atggtgccgc gcccgccctc gatatcggcg accagatcag
     2701 gatgatgcgc gtcgagcaga tgttcgtgat caaggtaata gccgccttcg gtcacggtaa
     2761 aggaaatcac gcgcgtacgc ggatcagcgc cgatcctgat caggtttttc aattgcgcat
     2821 cccagggcac gatgcggcgg atcgagcaga tcgtttcata ggcgcgcagg ccctgcggcg
     2881 tcaccgtctc cagcgtataa ctgcctttgc tttgcgtcag cgcctgcaat aacggcgcca
     2941 tgtcggcacg gatattggcc agtgccagtt gccagcggcg gtcttcgtca ttaccgcact
     3001 cacgcaggcg gtgcaggtag agcgcctgat gggcgcggtg aaatgatccg gcgccgatgt
     3061 gtaaccagac cagatcgttg actgaatcac gaatcgtcac cctctgcccc ccattgcgcg
     3121 cctgataagc aatccagtaa ccagagcggg taaatcacgc atatcgtcaa acaaagactt
     3181 tttccgcgcc ggtctcatac agctctttcg ccatgcccgc ccgatcgtgc gtaacgccgg
     3241 taaaacccag cacgcgcaac gcgatgtttt cactgtctaa cagaacgccg tcgcaatcgc
     3301 agaccagatg cgtgatgacg atgggaggtt cctgcattac tttaccgcgc caaaagtcag
     3361 tccctgcacc agctgcttct gcgccatcca gccaagcgcc atgatgggtg cg
//
LOCUS       DASRDH010000197         6328 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1187845,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000197 DASRDH010000000
VERSION     DASRDH010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 6328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6328
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1187845"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /locus_tag="VFA14_04490"
     CDS             <1..600
                     /locus_tag="VFA14_04490"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542740.1"
                     /note="catalyzes the formation of arginosuccinate from
                     citrulline and aspartate in arginine biosynthesis; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HZS80669.1"
                     /translation="PVAIGNKAFHDPVELVLEANRIGGRHGLGMSDQIENRIIEAKSR
                     GIYEAPGMALLHIAYERLVTGIHNEDTIEQYRDHGRRLGRLLYQGRWFDPQAIMLRET
                     AQRWVARAITGEVTIELRRGNDYSILDTESANLTYQPERLTMEKGEGAFSPQDRIGQL
                     TMRNLDISDTRQKLAIYQDTGLLASSAAVSLPLLEKNAK"
     gene            complement(664..1689)
                     /gene="murB"
                     /locus_tag="VFA14_04495"
     CDS             complement(664..1689)
                     /gene="murB"
                     /locus_tag="VFA14_04495"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007880289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HZS80670.1"
                     /translation="MLPLSVQRDCSLRHFNTFGIDVRAAACLPVDGIDTLLAVKNDRE
                     LSMLPRLILGGGSNLLLTQDFPGLVLHMRNTGMRIVGEDEVFVYVTAAAGENWHRFVQ
                     WSLNLGLGGLENLSLIPGSVGAAPIQNIGAYGVEVKDRFHSLTAFDFIDGKTLTLDHA
                     ACRFGYRDSIFRQELRDRAVVLDVSFALPKRWQASLHYADLMQELSAQQIVSPSPRDI
                     ADAVMTIRTRKLPDPAQVGNVGSFFKNPLVPAQQRDGLLARYPQLVSYAQPDGAYKLA
                     AGWLIDQCGWKGKSLGAAGVYEKQALVLVNKGGASGKEIVALAQVIQTDVRARFGVRL
                     EPEPVFI"
     gene            1813..2298
                     /locus_tag="VFA14_04500"
     CDS             1813..2298
                     /locus_tag="VFA14_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004189237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HZS80671.1"
                     /translation="MPSFDTVSEASLVEVRNAVDQANKEISARFDFKGSDARIEQKER
                     DLTVYADSEFQLGQARDVLTGKMSKRKVDLRFLDHGKIEKIGGDKVKQIIKVKNGIGT
                     EDAKKIVRVIKDSKMKVQASIQGDAVRVTGAKRDDLQAAMAMLRKEIADLPLEFNNFR
                     D"
     gene            complement(2310..4250)
                     /locus_tag="VFA14_04505"
     CDS             complement(2310..4250)
                     /locus_tag="VFA14_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010464141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HZS80672.1"
                     /translation="MSFDFVQINVFIRRLFYGSPACRCFRRLLYWIASAAVLALTACA
                     ATPSLESSPDAPLLPEPASGYQSKPGWAMGRFSIAAANPLATEAGYDILKRQGSAIDA
                     AIAVQMVLGLVEPQSSGIGGGAFLLYFDGKSIQAFDGRETAPAAVDETLFLDAAGKPM
                     AFYDAAVGGRSVGAPGAVRMLETAHRQYGKLPWAQLFDAAIRLAEEGFVVSHRLATML
                     KKNAYLRQDPQASVFYYPDGQALKVGAVLKNPAYAQVLRKIAAEGADGLMRGSVAQAI
                     VAKVNGHANNPGRLSVLDLARYEPKQREPMCSDYSARSPAGSVHAYRLCGMPPPGSGA
                     IAVAQILGLLERTQAHTLPLVEGSDPAKGPTPGADFLQLYMEASRLAYADRAKYLADP
                     DFVQAPGGSWYSLIAPGYLDQRASLIRTVSDAPGMQSVQAGAPGALAVSYAPMPAQQE
                     HGTSHISIVDADGNAVAMTTTIENAFGAGLLTDGGTGKAGGFILNNQLTDFSFAPADA
                     DGRPIANRVQAFKRPRSSMTPTLVFERASDGSNGKLVATLGSPGGGMIIHFTAKTLYG
                     MLNWGLNAQQAIDLPNFGSLGGPALLEEGRFTPATVQALRVRGAEVREIEMTSGLQAI
                     QRTPTGFFGGADPRREGVVRGD"
     gene            complement(4336..5688)
                     /locus_tag="VFA14_04510"
     CDS             complement(4336..5688)
                     /locus_tag="VFA14_04510"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZS80673.1"
                     /translation="MTFETLGLHPSIIKALTDSGYIKPTPVQEQAIPAAIAGKDLLVS
                     SQTGSGKTAAFMLPSLHKFTSMPPAAPGAKTANQEMQAARARGERPRFKPAQPKMLVL
                     TPTRELALQVTNATDKYCAHIRRLKAVSILGGMPYPKQMQLLSRNPEILVATPGRLID
                     HMDAGKIDFSELQILVLDEADRMLDMGFIDDIEKIVAATPASRQTMLFSATLDGVVGN
                     MARRITSTPQIIQITSSATRHENIAQRVHFVDDLSHKNRLLDYLLRDVTMDQAVVFTA
                     TKRDADTIADRLNIAGFTAAALHGDMHQGARNRTLNSLRRGQLRVLVATDVAARGIDV
                     PGITHVFNYDLPKFPEDYVHRIGRTGRAGRNGVAVSLVSHTEGMHVKRIERFTKQTIP
                     VDVVAGYEPRKTAAPARSARKPGGWRPGSSNRGGPPRSGNGNGGGYRSGGARRSYGDR
                     "
     gene            complement(6080..>6328)
                     /locus_tag="VFA14_04515"
     CDS             complement(6080..>6328)
                     /locus_tag="VFA14_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006464348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZS80674.1"
                     /translation="LGPRGVRVNGVSSGPIKTLAASGIKDFGKILGFVEDHAPLRRNV
                     TIEEVGNAAAFLLSDLASGITGEITYVDAGFSHIVGGM"
BASE COUNT         1386 a         1911 c         1857 g         1174 t
ORIGIN      
        1 cccgtcgcga ttggcaacaa ggcgtttcac gatccggtcg agctcgtgct cgaggccaac
       61 cggatcggcg gacgacacgg actggggatg agcgaccaga tcgaaaaccg gatcatcgaa
      121 gccaagagcc gcggcatcta cgaagcgccg gggatggcgc tgctgcacat cgcctacgag
      181 cgcctcgtga ccggcatcca caacgaggac accatcgagc agtaccgcga ccacggccgc
      241 cgtctcggcc gcttgctcta tcagggccgc tggttcgacc cgcaggcgat catgctgcgc
      301 gaaaccgcgc agcgctgggt cgcgcgcgcc atcaccggcg aagtcacgat agaactgcgg
      361 cgcggcaacg attactccat cctcgacacc gagtcggcca acctgaccta tcagccggaa
      421 cgcctgacca tggaaaaggg tgaaggcgct ttctcgccgc aagaccgcat cggccagctg
      481 accatgcgca atctcgacat cagcgatacg cgccagaagc tggcgatcta tcaagacacc
      541 ggcctgctag cgagcagcgc cgccgtgtcg ctgccgctgc tggaaaaaaa cgcgaaatga
      601 cttcagcatc gatcactttt agagcggctg acacaacgcc gctgttgcgt tgtgtcagcc
      661 gctctaaatg aacaccggct ccggctccag ccggacgccg aatcttgccc gcacatcagt
      721 ctgtatcacc tgcgccaggg cgacgatttc cttgccgctg gcgccgccct tgttgaccag
      781 caccagcgcc tgtttttcat acacgccggc tgcgccgagc gacttgccct tccagccaca
      841 ttgatcgatc aaccatccag ccgccagttt gtaagcgcca tcgggctgcg cgtaactcac
      901 cagttgcgga taccgcgcca gcaggccgtc gcgctgctgc gccggcacca gcgggttctt
      961 gaagaagctg ccaacattgc caacctgcgc cgggtcaggc agtttacgcg tgcggatcgt
     1021 catcaccgca tcggcaatgt cgcgcggcga tggcgatacg atttgctgcg ccgacaactc
     1081 ctgcatgagg tcggcataat gcagactggc ctgccatcgt ttgggcaaag cgaaactcac
     1141 atcgagtacc accgcgcgat cgcgtaattc ctgcctgaaa atactgtcgc gatagccaaa
     1201 gcgacaagcg gcatggtcca gcgtcaaggt tttgccatcg atgaaatcga atgcggtcaa
     1261 actgtggaaa cggtctttga cttcaacccc atacgcgccg atgttctgga tcggcgccgc
     1321 gccgacgctg ccgggaatca gggagaggtt ttccagcccg ccgaggccga gattcaacga
     1381 ccactgcacg aaacgatgcc agttttcgcc agcagcggcg gtcacgtata cgaaaacctc
     1441 atcttcaccg acgatacgca taccggtgtt gcgcatgtgc agcaccagcc caggaaaatc
     1501 ctgcgtcagc agcagattgc taccaccacc gagaatcagg cgcggcagca tcgacaactc
     1561 cctgtcgttc ttgacggcga gcaaggtatc gatgccatcg accggcaagc aggcagccgc
     1621 tctgacatcg atgccgaaag tattgaaatg gcgcagggag caatcccgct ggactgaaag
     1681 gggaagcata agcgtagaat tatagcgtag cgactgccgc tgacgtagcc gccatccctc
     1741 tggtccgtta aaatcacgtc tttcgggcgt ccacaagttc tcaatgccca cagaatgaca
     1801 agggaaacta ccatgccatc gtttgatacc gtctccgaag ccagtctggt cgaagtcagg
     1861 aatgccgtcg accaagccaa caaggaaatc agcgcccgct tcgacttcaa aggcagcgac
     1921 gcccgcatcg aacagaagga gcgcgatctg acggtctatg ccgactccga attccagctc
     1981 ggtcaggcgc gcgacgtgtt gaccggcaag atgagcaagc gcaaggtcga tctgcgtttt
     2041 ctcgaccatg gcaagatcga aaagatcggc ggcgacaaag tcaagcagat catcaaggtc
     2101 aaaaacggta tcggcaccga agacgccaaa aagatcgtgc gcgtcatcaa ggacagcaag
     2161 atgaaagtgc aagccagcat ccagggcgac gccgtgcgtg tcaccggcgc caagcgcgat
     2221 gacctgcaag ccgccatggc aatgctgcgc aaggagatcg cggatttgcc gctggagttc
     2281 aacaacttcc gcgactgatc atcacaggtt caatcccctc gaacgacgcc ttcccggcgc
     2341 ggatccgcgc cgccgaaaaa acccgtcggc gtccgttgaa tcgcctgtag tccgctggtc
     2401 atctctattt cccgcacctc agcgccacgt actcgcagcg cctgaaccgt ggccggcgtg
     2461 aagcggcctt cttcgagcag agcaggacca ccgagcgatc cgaaattggg cagatcgatc
     2521 gcctgctgcg cgttcaaacc ccagttgagc atgccataca gggtcttggc cgtaaaatga
     2581 atgatcatgc cgccgccggg actgccgagg gtggcgacga gtttgccgtt gctgccgtcg
     2641 ctggcgcgtt caaacaccag ggtaggcgtc atggatgaac gcgggcgttt gaaggcctgg
     2701 acgcggttgg cgataggtcg accatcggca tcggcgggcg cgaagctaaa atccgtcaac
     2761 tggttattga gaataaagcc gcctgccttg ccggttccgc catcggtgag caaacccgcc
     2821 ccaaaggcat tctcgatcgt cgtcgtcatc gccaccgcat taccatcggc atcaacgatg
     2881 ctgatgtggg aagtgccatg ttcctgctgc gccggcatcg gcgcatacga gaccgccaga
     2941 gcgccgggcg cgccagcttg tactgactgc atgccaggcg catcagacac ggtccggatc
     3001 aagctggcgc gctgatccag gtagccgggc gcgatcagac tgtaccaact cccgcccgga
     3061 gcctgcacga aatcgggatc cgccagatac ttcgcccggt cggcataggc cagacgcgaa
     3121 gcttccatgt agagctgcag aaaatccgcg ccgggcgtcg gccctttggc aggatcgcta
     3181 ccttcgacca gcggcagcgt atgggcctgc gtgcgttcca gcaggccgag aatctgcgct
     3241 acggcgatcg caccggagcc aggcggcggc atgccgcaca ggcggtaggc atgcacgctc
     3301 cccgccggac tcctggcgga gtagtcgctg cacatcggtt cacgttgctt cggttcataa
     3361 cgcgcgaggt caagcactga caatcggcca ggattgttgg cgtgtccgtt gaccttggcg
     3421 acgatggctt gcgccacgct accgcgcatc agaccatcgg ccccttccgc agcaattttg
     3481 cgcaacacct gcgcgtaggc gggatttttc agaaccgcac cgactttcag ggcctgacca
     3541 tcggggtagt agaagaccga agcctgcgga tcctgacgta aataagcgtt cttcttcagc
     3601 atggtcgcga ggcgatgact cacgacgaat ccttcctctg ccaggcggat cgctgcatcg
     3661 aacaactgcg cccaggggag ttttccatac tgtcgatgcg ctgtttccag catgcgcaca
     3721 gcgcccggcg cgccaacgga gcgccctccc acggcggcat cgtagaaggc cataggcttg
     3781 ccggcagcat cgaggaacag cgtttcatcg actgcggccg gggcggtctc acgaccatca
     3841 aatgcttgaa tggatttacc atcgaaatag aggagaaaag cacctccgcc gataccgctg
     3901 gattggggtt cgaccaggcc cagcaccatc tgcaccgcaa ttgcggcatc gatcgccgaa
     3961 ccttggcgtt tgagaatgtc gtagcccgcc tcagtcgcca gcggattggc ggcggcgatg
     4021 gaaaacctgc ccatcgccca gccaggcttg ctttgataac cggaagcggg ttccggcaag
     4081 agcggcgcat ccgggctgga ctccagagaa ggagtggctg cgcaagcggt cagcgccaga
     4141 acagcggcac tggcgatcca atagagaagc cttcggaaac agcggcaggc agggctaccg
     4201 tagaacaagc gacggatgaa gacattgatc tgcacaaaat cgaaagacat ggcatcccct
     4261 tatgatcatg acgatagttg atcatatgtc tggatatgct ttcggtaaaa gcagcatcaa
     4321 tcgccgaagg tatggtcagc gatcgccgta agagcgtcgg gcgccgccgc tcctgtaacc
     4381 gccgccatta ccattacctg aacgcggcgg accgccgcgg ttgctgctgc ctggacgcca
     4441 gccgcccggt ttgcgcgcgc tgcgtgcagg cgctgccgtt ttcctgggtt catagcctgc
     4501 cacgacatcg accggaatcg tttgtttggt gaagcgttcg atacgtttga cgtgcatgcc
     4561 ttcggtatga ctgaccagcg acacggcaac gccgttgcgg ccggcgcgac cggtacggcc
     4621 aatgcggtga acatagtctt ccggaaattt cggcagatcg taattgaaca cgtgagtgat
     4681 gcccggcaca tcgatgccgc gcgcggcgac atcggttgcc accagcacac ggagctgacc
     4741 gcggcgcaga ctgttcagcg tacggttgcg cgcgccctgg tgcatgtcgc catgcagggc
     4801 ggcggcagtg aaaccggcga tgttcaggcg atcagcgatg gtgtcggcat cacgcttggt
     4861 cgcagtgaag accacagcct gatccatggt cacatcgcgc agcagatagt ccagcaggcg
     4921 atttttatgc gacagatcat cgacgaagtg gacgcgctgg gcaatgtttt catgcctggt
     4981 ggcggaactg gtgatctgga tgatttgcgg cgtgctggtg atgcgtctgg ccatgttgcc
     5041 gaccacacca tcgagcgtcg ccgagaacaa catggtctgg cggctggccg gcgttgcggc
     5101 gacgattttt tcgatgtcgt cgatgaagcc catgtccagc atgcggtcgg cttcatccag
     5161 caccaaaatc tgcagttcag aaaaatcgat cttgcccgca tccatatgat cgatcagacg
     5221 accgggcgtc gccaccagga tttccggatt gcgcgacagc aattgcatct gcttcggata
     5281 aggcatgccg cccaggatgg atacggcttt cagacggcgt atatgggcgc agtacttatc
     5341 ggtggcgttg gtcacttgca gcgccagttc gcgggtcggg gtcagcacca gcatcttcgg
     5401 ctgggccggc ttgaagcgtg gccgctcgcc gcgcgcacgc gcggcctgca tttcctggtt
     5461 cgctgttttg gcaccgggcg cagccggcgg catcgaggta aatttgtgca gcgatggcag
     5521 catgaatgcc gcagtcttgc cggagcctgt ctgcgacgac accagcagat ccttgccagc
     5581 aatggcagca ggaatggctt gctcctggac cggagtcggt ttgatatagc cggagtcggt
     5641 caaggctttg atgatcgacg ggtgtaggcc tagcgtttca aaagtcatgt tttctttcgt
     5701 tcatggatgc atatcagaca catgccatgc agcgtaacga tggcgggcat gaatagcagc
     5761 gatcgcaacg cgcacatggc aaatgcaatg aacaccaaaa agcaacgcca accaaggaaa
     5821 tgactatgaa agaccaagaa atttcggcgc aaaagctttg cgccgggacg gcgtcactcc
     5881 aagatcgagg acgcccgagg cgggaacgct tttactacat actgtcattt atctggtaga
     5941 caataacagt cagggcttgc gaagctgctt agtgactgga ttgccatagc gatgacaggt
     6001 acaacccagt tcgcgcctcg cattgcaagg cacgaagcgc atcatacagg aaattatcga
     6061 atcgtattta aaatttattt cacattccac ctacgatgtg tgagaagcca gcatccacgt
     6121 aggtgatttc accggtaata ccgctcgcca gatccgacag caggaaagcc gctgcattgc
     6181 ccacctcctc gatcgtgacg ttgcggcgca acggagcgtg atcctccaca aagccgagta
     6241 tcttgccaaa gtccttgatg ccgctggcag ccagggtctt gatcggaccg gacgagacgc
     6301 cgttgacgcg cacgcctctg gggcccag
//
LOCUS       DASRDH010000198         3493 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_173657,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000198 DASRDH010000000
VERSION     DASRDH010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 3493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3493
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173657"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..407
                     /locus_tag="VFA14_04520"
                     /pseudo
     CDS             1..407
                     /locus_tag="VFA14_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014618896.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
     gene            complement(581..1237)
                     /locus_tag="VFA14_04525"
     CDS             complement(581..1237)
                     /locus_tag="VFA14_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3299 domain-containing protein"
                     /protein_id="HZS80675.1"
                     /translation="MKMLLWIAGIVVAGLLAGHAARLLPTLQAEPVSTVDTPYQVGDH
                     LRKIPDLSKKSEQASDKSVGKFREITWESLMPKNWDAMAPFKGLKLDTLEDSDPRAME
                     ALWKARKYWKNAPVDPGMDGAAIRIPGFVVSLEREGAALKEFLLVPYFGACIHVPPPP
                     ANQIIHVRNSKPLNDIRTMDAVWISGVLKVEHSDTMMGAAGYSMASIEIEPYTDPTPQ
                     "
     gene            complement(1242..2504)
                     /locus_tag="VFA14_04530"
     CDS             complement(1242..2504)
                     /locus_tag="VFA14_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZS80676.1"
                     /translation="MSFMFRLATRSAWSRRFTLGLMLLAIALSSAMLLGIERVRHEVR
                     TGFSQSVSGADLVVGARTSAVQLMLYAIFRIGGVTNNMGWGSAQKLAKNPAVAWTIPL
                     SLGDSHRGFPVLATNGDYFTHFHYGDGQALQFTEGRVFDSVFDTVIGAEVAQRLHYRP
                     GDRIVLSHGIGNPRLADHANKPFTITGILAQTGTPIDRTVHIGLDAMEAIHLDWGGGA
                     PMPGLSIAPALVKKFDLTPKTITAVLVGLKNRAAVFGVQRDIADDSSEALMAVLPGVA
                     LDELWQVVGVGENVLLVVSGMVVIVGLAGLLSAILASLGERRRELAVLRSVGARPADI
                     FLLLALEGLTVMLAGVLLGIVLLTLALFALGPFLSAQFGVTLHTSLPAASEWQLLLWT
                     ILAGFVTNLLPGLRAYRLSLADGLTPGI"
     gene            complement(2501..3214)
                     /locus_tag="VFA14_04535"
     CDS             complement(2501..3214)
                     /locus_tag="VFA14_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016836290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZS80677.1"
                     /translation="MNDAAIALRDIRFTWPGQKTPALVLDRFTVEAHAQVFVSGPSGS
                     GKSTLLALIGGIASPQIGSVAILGQTLEDLPASRRDRFRADHIGFIFQQFNLIPYLSI
                     LDNVLLPCRFSAYRRARALAQGKDLHAAAQSLLRSLDLTPSLWARAVTQLSIGQQQRV
                     AAARALIGRPEIVVADEPTSALDSNRQQAFLDLIQHECEQAGASLVFVSHDQRLAQRF
                     HRCITLNDIDPCANDGAAA"
     gene            complement(3237..>3493)
                     /locus_tag="VFA14_04540"
     CDS             complement(3237..>3493)
                     /locus_tag="VFA14_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007882683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2796 domain-containing protein"
                     /protein_id="HZS80678.1"
                     /translation="AAIEPALLGETASNKTGLHADQGSHADLHADFHFQCAQPGQLQR
                     IDVKLFDAFERFDRIDVQLVTPRRQGATTLTPAASGIFLP"
BASE COUNT          740 a         1027 c         1042 g          684 t
ORIGIN      
        1 atgcgcagat agccgtggtc ggtcggctcg cggccgatat cattgcacga cacgaaatcc
       61 tgccacagaa ggtcgtcgga cattctgatg ttgcaccagg acgcaagtgg gatccgggtc
      121 cgttgtttcc gtggcaaaaa ctctataccc aatatgggat cggggcctgg ccagaagcgg
      181 aagcaattag ctattaccgt agccatcaac cgttccgcgg tgacatcggc gcgttacaga
      241 ggaaactcct cgcgtatggc tacgatacac cgcagaccgg cgtactcgat gcgcaaactg
      301 gcaacgtcgt cgcggcgttc cagatgcatt ttcgttccgc gaaatatgac ggcgtgcccg
      361 atgtcgagac ggtcgcaatc cttgatgcac ttctggaaaa gtacttttga cgcggtcgca
      421 tgcagttacg tcaagtggcg cctgagcaac ttgagccaca aaatgaaagt gaaaaaggta
      481 gggtagtagt actccgccct cgtcaggcgc aatcggacag ctggtattca caggcttgtt
      541 cccaaaatat ggggataatt ttctgtcatg cgcgggcagg ctactgcggc gtcgggtccg
      601 tgtaaggctc gatctcgatc gacgccatgc tgtagccggc agcacccatc atcgtgtccg
      661 aatgttccac tttcagcacg ccgctgatcc ataccgcatc catggtgcgg atgtcgttga
      721 gcggtttgct gttgcggacg tggatgatct gattggccgg tggcggcgga acgtggatgc
      781 aggcgccgaa atagggtacc agcagaaatt cctttaaggc tgcgccttcg cgttccagcg
      841 atacgacaaa gccggggatg cggatcgccg cgccgtccat gcccggatcg accggcgcat
      901 ttttccagta tttcctggct ttccatagcg cctccatggc acgcggatca ctgtcttcca
      961 gcgtgtccag cttcaatccc ttgaatggtg ccatggcatc ccagtttttc ggcatcaagg
     1021 attcccaagt gatctcgcga aattttccca cgcttttatc ggaagcttgc tcagattttt
     1081 tcgataaatc aggaattttc cgcagatggt cgccgacctg atatggcgtg tcgacggtgg
     1141 aaaccggctc ggcttgtagc gtgggcagca gacgcgccgc atgaccggcc agcagtccgg
     1201 cgaccacgat gccggctatc cagaggagca ttttcatgat ctcaaattcc cggagtcagg
     1261 ccatcggcca ggctgagccg gtaagcacgc aggcccggca acagattggt aacgaaaccg
     1321 gcaaggatgg tccacagcaa taattgccat tcgctggcgg ccggtaagct tgtgtgcaag
     1381 gttacgccaa actgcgcgct gaggaagggt cccaacgcaa acagcgccag cgtcagtaaa
     1441 acgatgccca gcagcacgcc agccagcatc actgtcagtc cttccagcgc cagtagcaga
     1501 aaaatatctg ctggccgcgc gcccaccgag cgcagcactg ccagttcgcg gcgccgctcg
     1561 cccaggcttg ccagaatcgc cgacagcagt cccgccaggc cgacgatcac taccatgccg
     1621 gacaccacca gcaagacgtt ttcgccgacg ccgacgactt gccacagctc gtccagcgcc
     1681 acgccgggca acaccgccat cagcgcttcg ctgctgtcat cggcgatatc gcgctgcacg
     1741 ccgaaaacgg cagcacggtt tttcagaccg accagcacgg ccgtgatggt cttgggcgtg
     1801 aggtcgaact tcttcaccag cgccggcgcg atcgacaggc cgggcatcgg cgccccgcct
     1861 ccccaatcca ggtggatggc ctccatcgca tcgaggccga tgtgtacagt gcggtcgatc
     1921 ggcgtgccgg tctgtgccag gatgccggtg atggtaaatg gtttattggc atggtcggcc
     1981 aggcgcgggt tacctatgcc gtggctcagc acgatgcggt caccgggtcg gtaatgcagg
     2041 cgttgcgcga cttcagcgcc gatgacggtg tcgaacacgc tgtcgaagac gcggccttcg
     2101 gtaaattgca acgcctgtcc atcgccataa tggaaatgcg tgaaataatc gccattggtc
     2161 gccagtacgg gaaaaccgcg atgcgaatcg cccaacgaca gcggaatcgt ccacgccacg
     2221 gcgggatttt ttgccagctt ttgcgcactg ccccatccca tgttattggt gacgccacca
     2281 atgcggaaga tcgcatacag catcaactgg accgcgctgg tgcgtgcgcc gactaccagg
     2341 tcggcacccg agaccgattg cgaaaaaccg gtgcgcactt catggcgtac acgttcgatg
     2401 cctagcagca tagcgctcga caaagcaatc gccagcagca tcaggccgag cgtaaaacgg
     2461 cggctccacg cgctgcgcgt ggcaaggcga aacataaaac tcatgctgcc gccccatcat
     2521 tggcgcaagg atcgatgtcg ttgagcgtaa tgcagcgatg aaaacgttgc gccagccgct
     2581 gatcgtggct aacgaacacc agactggcgc cggcctgttc gcattcgtgc tggatcagat
     2641 cgaggaaggc ttgctggcgg ttactgtcga gtgctgaagt cggttcgtcg gcaaccacga
     2701 tttccggcct gccgatcagc gcgcgcgcag cggccacacg ctgctgctgc ccgatcgaga
     2761 gttgtgtgac cgcgcgcgcc cacaaactag gcgtcaaatc gagactgcgc aacaacgatt
     2821 gtgctgccgc atgcaaatct ttgccctgtg ccagggcacg cgcgcggcgg taagcggaaa
     2881 accggcaagg caacagtaca ttgtcgagaa tcgacagata ggggatcagg ttgaactgct
     2941 ggaagatgaa gccgatatga tcggcgcgaa aacggtcacg gcgcgacgcc ggcaaatcct
     3001 ccagcgtttg tccgaggatg gcgacgctgc cgatttgcgg gctggcaatg ccgccgatca
     3061 gcgccagcag tgtacttttg ccgctgccgc tgggaccact gacgaagact tgtgcgtgcg
     3121 cctccacggt gaagcgatcc agcaccagcg ccggtgtttt ctggccgggc caggtgaagc
     3181 ggatatcacg taatgcgatg gcagcgtcat tcatgctcgg ggggtgctgc aatgcatcag
     3241 ggaaggaaga tacccgatgc cgccggtgta agtgttgtcg cgccttgacg cctgggcgtc
     3301 accagttgca cgtcgatgcg gtcgaacctc tcaaaggcgt cgaacagctt tacgtcgatg
     3361 cgctgcaact gcccgggctg cgcgcattga aagtgaaagt cggcatgcag atcggcgtga
     3421 ctaccctgat ctgcatgcaa gcctgtttta ttggaggctg tttcacccag caatgccggt
     3481 tcgatggcgg cag
//
LOCUS       DASRDH010000199         4500 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_580495,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000199 DASRDH010000000
VERSION     DASRDH010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 4500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4500
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_580495"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2487
                     /gene="dnaE"
                     /locus_tag="VFA14_04545"
     CDS             <1..2487
                     /gene="dnaE"
                     /locus_tag="VFA14_04545"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008116968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HZS80679.1"
                     /translation="KERPRYEARLQFENETIIKMKFSGYFLIVADFIRWAKNNGVPVG
                     PGRGSGAGSLVAYALSITDLDPLEYNLLFERFLNPERVSMPDFDIDFCQEGRDRVIQY
                     VKDCYGKEAVSQIATFGTMAAKGAVRDVGRVLDLGYNFCDGISRLIPFKPGKLVTIAD
                     AIEEEPLLKERLENEEEVRQLLQLAQQVEGIARNIGMHAGGVLIAPGKLTDFCPLYTQ
                     GGDAGVVSQYDKDDVEAIGLVKFDFLGLTTLTILDRAVRYIKQLDPAMADFDLARLSL
                     TDRASYELLSRARTVAVFQLESRGMQGMLKDARPDRFEDIIALVALYRPGPMDLIPDF
                     CKRKHGEKFDYPDPRTESILSETYGIMVYQEQVMQMAQVIGGYSLGGADLLRRAMGKK
                     KAEEMAEHRNIFREGAARNGLSQEKADEIFDLMEKFAGYGFNKSHAAAYALLSYHTAY
                     LKVHHPAAFMAANLSLAMDDTDKIRILVEDALDVCGLDLLPPDINLSDYRFTPVGEPG
                     KKAMQIRYGLGAVKGSGQSAIEAIIAARQEGPFKDLFDFAGRVDRKQINRRTIDSLIR
                     AGAFDCFGEDRAVLQASVGLAWESAEQAQAFANQVSLFGGIEETWLEYVQAGHWSDRQ
                     RLVEEKTALGFFLSGHLFDAYEKEVRRFVRTRISSLEPSRDSRLLAGIITGIRVQLTQ
                     RGKLLIVTLDDGSGTADVTVYGELAEPNKAFFKEDELLIVQGKVSEDRFSSGLRVSAE
                     KVMDIGAARIQYGRRVVVSLAGSVDPAHLRDILSSYRRQQGLPFVMKYIQSGVGCEVM
                     LGDDWRVVPSDGLQASLCESFGPEAVAVEY"
     gene            2625..2894
                     /gene="rpsO"
                     /locus_tag="VFA14_04550"
     CDS             2625..2894
                     /gene="rpsO"
                     /locus_tag="VFA14_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012401401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HZS80680.1"
                     /translation="MSLEKAAKAAIITDNARARNDTGSPEVQVALLTARINDLNAHFK
                     EHSKDHHSRRGLIKMVNRRKSLLSYLKGKDADRYRALIEKLGLRK"
     gene            3097..>4500
                     /locus_tag="VFA14_04555"
     CDS             3097..>4500
                     /locus_tag="VFA14_04555"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008327631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HZS80681.1"
                     /translation="MFDKVTKTFQYGQHQVTLETGEIARQASGAVVVSIEDTVVLATV
                     VARKDARPDQDFFPLTVDYIEKTYAVGRIPGGFFKREGKPSEKETLTSRLIDRPIRPL
                     FPESYRNEVQVIVHVLSVNPEIDPDIVAMIGASAALSIAGIPFSGPVGAARVGYIDGQ
                     YVLNPTTTQLKSSQLDLVVAGTETAVLMVESEARQLSEEIMLGAVVFGHEQSKVVIDA
                     IHELVRDGGKPEVQWAPAPKNEALIARVTELADGKLREAYQTRDKQARTEKLRAVTAE
                     VNAALAADAVEVGGILFDLEARIVRSQILDGEPRIDGRDTRTVRPISIRTGVLPRTHG
                     SALFTRGETQALVIATLGTARDEQKIDALMGEYSDRFMLHYNMPPFATGETGRVGTPK
                     RREIGHGRLAKRALAAALPPAEEFSYSVRLVSEITESNGSSSMASVCGGCLALMDAGV
                     PVQAHVAGIAMGLIKEGN"
BASE COUNT          928 a         1259 c         1354 g          959 t
ORIGIN      
        1 aaagagcgtc cgcgttacga ggcgcgtctt cagttcgaga atgagaccat catcaagatg
       61 aaattttccg gctatttcct gatcgtggcc gatttcatcc ggtgggccaa gaataatggt
      121 gtaccagtcg ggccgggtcg aggctcgggc gccggttcgc tggtggcgta tgcgctgtcg
      181 attactgacc tcgatccgct ggaatacaac ctgctgttcg aacgttttct caatcctgag
      241 cgggtctcga tgcctgactt cgacatcgac ttttgccagg aagggcgtga ccgcgtcatt
      301 cagtatgtca aggactgtta cggcaaggag gcagtctcgc agatcgccac cttcggcact
      361 atggcggcca agggggcggt gcgcgatgtc ggtcgcgtgc tcgatctcgg ctacaatttt
      421 tgcgacggca tttccaggtt gatcccattc aagccgggca agttggtgac aatcgccgac
      481 gccatcgaag aagagccgct gctcaaggag cgcctggaga acgaagaaga ggtcaggcag
      541 ctgctccagc tggcgcagca ggtcgaaggg attgcccgta acatcggcat gcatgccggc
      601 ggtgttctga tcgcgcctgg caagctgacc gacttttgtc cgctctatac ccagggcggt
      661 gatgctggcg tggtgtcgca gtacgacaag gacgatgtcg aagccatcgg cctggtcaag
      721 ttcgattttc tgggactgac cacgttgacg attctcgatc gcgctgtgcg ttacatcaaa
      781 cagcttgatc cggccatggc ggatttcgat cttgccaggc tgtcgctgac cgaccgcgct
      841 tcctatgaac tgttgagcag agccagaaca gtcgcggtat tccagctgga aagccgcggc
      901 atgcaaggca tgctcaagga tgcgcgtccg gaccgtttcg aagacatcat tgcactagtg
      961 gccctgtacc gcccgggtcc gatggatctg attcccgact tctgcaaacg caagcacggc
     1021 gaaaagttcg actatcccga tccgcgcacg gaaagcattc tttccgagac ttacggcatc
     1081 atggtctatc aggagcaggt catgcagatg gcgcaggtga tcggtggcta ttcgctcggc
     1141 ggcgccgatc tgctgcgccg tgcgatgggc aagaaaaaag ccgaagaaat ggccgaacac
     1201 cggaatatct tccgcgaagg cgccgccagg aatggtctgt cgcaagagaa ggccgacgaa
     1261 atcttcgacc tgatggaaaa attcgctggt tacggcttca acaaatcgca cgccgccgct
     1321 tatgcgctgt tgtcttacca cactgcctat ctcaaggttc atcaccccgc cgcgttcatg
     1381 gcagccaacc tgtcgctggc catggacgac accgataaaa tcaggattct ggtcgaagac
     1441 gcgctcgatg tctgcggcct cgacttgctg ccgcccgaca tcaatctgtc ggattaccgc
     1501 ttcacgccgg tcggtgagcc gggcaagaaa gccatgcaga tccgctatgg cctgggtgcg
     1561 gtcaagggtt ctggccaaag cgcgatcgaa gccatcattg ctgcgcgtca ggagggaccg
     1621 ttcaaggact tgttcgactt cgccgggcgc gtcgacagga aacagatcaa ccgccgcacc
     1681 atcgactctt tgattcgcgc cggcgctttc gactgcttcg gcgaagaccg cgcggtgctg
     1741 caagcttcgg tcggtctggc atgggaaagc gccgagcagg cgcaggcttt tgccaatcag
     1801 gtcagcctgt tcggcggcat cgaggaaaca tggctggaat atgttcaggc gggccactgg
     1861 agtgataggc agcgccttgt cgaagagaag accgcgctcg gtttcttttt gtcgggacat
     1921 ttgttcgacg cctatgaaaa agaagtacgc cgattcgtgc gtaccaggat ttccagtctg
     1981 gaaccgtcgc gcgactcgcg cctgttggcc ggcatcatca ccggcattcg tgtacagctg
     2041 acccagcgcg gcaaactgct gatcgtcacg ctcgacgatg gcagcggcac tgctgatgtc
     2101 acggtctacg gcgaacttgc cgagcctaat aaagcctttt tcaaggagga tgagttgctg
     2161 atcgtacagg gcaaggtgtc ggaagatcgt ttctccagcg gcttgcgcgt ctctgctgaa
     2221 aaggtcatgg acattggcgc ggcgcgtatc cagtacggcc ggcgtgtggt ggtgtcgctg
     2281 gccggaagcg tcgatccggc gcatctgcgt gacatactgt catcctaccg ccggcagcag
     2341 ggattgccgt tcgtgatgaa atatattcag tccggcgtcg gctgcgaagt catgctgggt
     2401 gatgactggc gcgtggtgcc gagcgatgga ttgcaggctt cgctgtgcga gtcgtttggg
     2461 ccggaagcag ttgcggtcga gtattgacgg ccatttgaat tttttggcct tttactatac
     2521 aatctcgcgt ctggtcgttt cagacagtat ggtttttgtt cacggtatgc agggtagcca
     2581 ctcctggaat agtcactccg tataccgctt gtgaaaggta tgatatgtcg ctcgaaaaag
     2641 cagccaaggc tgccattatt actgataacg cccgcgcccg gaacgacacc ggctccccgg
     2701 aagttcaggt tgcgctgctc acggcgcgca tcaatgacct caacgcccac ttcaaggagc
     2761 acagcaagga tcatcattcc cgtcgcggac tgatcaagat ggtcaatcgc cgcaagagcc
     2821 tgctgtccta tctgaagggc aaggatgccg accgttatcg cgcattgatc gaaaaactcg
     2881 gtttgcgcaa gtaatcttga cccttcgcgt cattccggtt cagggtctat ggccgaaatg
     2941 cccgcgtcag tttctgacgt gggcattttg ttttcttgtg gttctgcttt gctggttcat
     3001 tcgtggcgag gcgagcgaca gcgcctgaaa atctgtcggc aaaaaaattc ctgcaagggc
     3061 cgccgcgcaa gggttcaaca tagaaaggaa agcaccgtgt ttgataaagt taccaaaacc
     3121 ttccagtatg ggcagcacca ggtcacgctg gaaaccggcg aaatcgctcg ccaggcttcc
     3181 ggcgcagtcg tcgtttcgat cgaggacacc gttgtgctgg cgaccgtcgt ggcgcgcaag
     3241 gatgccaggc cggatcagga ttttttccca ttgaccgtcg attacattga aaagacctac
     3301 gctgtgggcc gtatccctgg cggttttttc aaacgtgaag gcaagccgtc cgaaaaggag
     3361 acactgacat cgcgcctgat cgaccgcccg attcgtccgt tgttcccgga aagttaccgg
     3421 aacgaagtgc aagtcatcgt ccacgtgttg tccgtcaatc ctgaaatcga tccggacatc
     3481 gttgcgatga taggcgcgtc ggcagcgttg tcgatcgccg gcattccgtt cagcggtccg
     3541 gtcggcgccg cacgtgtcgg ttacatcgac ggccagtacg tgctgaaccc gaccaccacg
     3601 caattgaagt cttcgcaact ggatctggtt gtcgccggca ccgaaaccgc tgtgctgatg
     3661 gtcgaatctg aagcgcggca actatctgaa gaaatcatgc tgggcgcagt cgtgtttggt
     3721 cacgagcaat cgaaagtagt catcgatgcc atccatgaac tggtgcgcga tggcggcaag
     3781 ccggaagtcc agtgggcgcc cgcgccgaaa aacgaagcgc tgatcgcgcg cgtcaccgaa
     3841 ctggccgacg gcaagctgcg cgaagcgtat cagaccaggg acaagcaggc ccgtaccgaa
     3901 aagctgaggg cagtcactgc cgaagtcaat gctgcgctgg cagcggatgc cgtagaagtc
     3961 ggcggcatcc tgttcgacct ggaagccagg atcgtccgtt cgcaaatcct cgatggtgag
     4021 ccacgtatcg atggccgtga cacacgcact gtacgtccga ttagcatccg taccggcgta
     4081 ctgccgcgca cccatggttc cgcgctattc acccgcggcg aaacgcaggc gctggtcatc
     4141 gcgaccctgg gtacggcgcg cgacgagcaa aagatcgacg cgctgatggg cgagtactcg
     4201 gaccgcttca tgctgcacta caatatgcct ccgttcgcga ccggcgaaac tggccgtgtc
     4261 ggtacgccga agcgccgtga aatcggtcat ggccgtctgg ccaagcgcgc gctggccgct
     4321 gcattgccgc ccgcagagga atttagctac tccgtgcgtc tggtgtcgga aatcaccgaa
     4381 tccaacggtt cttcatcgat ggcttccgtc tgtggcggtt gcctggcatt gatggatgcc
     4441 ggcgtgccgg tgcaggcgca cgttgccggt atcgccatgg gcctgatcaa ggaaggcaac
//
LOCUS       DASRDH010000200         2289 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1553669,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000200 DASRDH010000000
VERSION     DASRDH010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1553669"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="VFA14_04560"
     CDS             <1..319
                     /locus_tag="VFA14_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS80682.1"
                     /translation="QPAGSSQYRIAAMAFDGHISHMLRPVGKYFHDNPQFDGVDFTTT
                     VHQSAQPTSISVEFVVPFSALACYEKYDCTGQELINRSIVLINGERVALDLQRAEADS
                     LVK"
     gene            594..1302
                     /locus_tag="VFA14_04565"
                     /pseudo
     CDS             594..1302
                     /locus_tag="VFA14_04565"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            1571..>2289
                     /locus_tag="VFA14_04570"
     CDS             1571..>2289
                     /locus_tag="VFA14_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS80683.1"
                     /translation="MKFVSFLVTFFCATALFAVQPPQSATTPLPNINFTSVGTVSSVQ
                     LTGNANWVFGSDQQTGTVTLQANAAGQSRMLLQLSSGPRLETQNAFSDSTRQCTWSGI
                     DAVVHTSAVHHCWVDAIWFLPQITMQIGAGASDDSASATAAPDGKIVHIHHERHPVGV
                     RDVGTANLLAHLSAVDLDIDATTGFPLELNFAAHPDNDAGTDISVQVQFSDYRSVNGV
                     TIPFHIQKFINNSLVLDLQIST"
BASE COUNT          578 a          589 c          569 g          553 t
ORIGIN      
        1 acagcccgcc gggtcgtcac aataccgcat cgctgccatg gcttttgacg gccacatctc
       61 gcacatgctg cggccggtgg gaaaatactt ccatgacaac ccgcagtttg acggagttga
      121 tttcaccacc acggttcacc agtcggcgca acccaccagc atatctgtag agtttgtggt
      181 gccgttctct gccctggctt gctatgagaa gtatgactgc acaggccagg aactgatcaa
      241 ccgcagcatt gtccttatca atggagaaag ggttgctttg gacctgcagc gcgcagaagc
      301 ggattctctc gttaaatgag atagagcagc catgctgccg atgcttcgca ggctgagaat
      361 gacgatgcct ttttcgtgaa atccattcgc cacttcaatt cgccggatgc cattgtcact
      421 ggtttttcgg ctgctcccag attactcgga catgcgaagt tccgagccgc agaagtccaa
      481 gtctggttag ggcagcaatc ccacatgtcg aagcctactc acatactgac cgatgatgct
      541 gacattgctg ccgctctcaa agagtcgatc tcagttatga aacgaaagaa gtaatgtgcg
      601 gacggtacag actcacggcg aaggaacgct ggctccgcga gtatttcaac atccctgctg
      661 aggacataga ttgggccgct cgatggaata ttgcacccac ggatgaggtc gcaacgatcc
      721 gccaatcgcc acgggccgaa gagaatcttc gcgaagatgc gttgggggct gattccctat
      781 tgggcaaaag acaaatcctt cggggccaag gcgatcaacg ctgtctccga gacggcggct
      841 gagaaaccag cattcaaaga ttcgatgaag aagcgtcgtt gcctgatcct ggcagacggc
      901 ttctacaaat ggaagaagct ctgcagcaaa aagcagccgt acaacatcgg catgaaggat
      961 ggcggcctgt ttgcgttctc cggattgtgg gacagatgga aagaccctga cggcaaggtc
     1021 atcgagtcct gcaccattct gacgacagaa gcgaatccgt tgctcaaaga cattcacgat
     1081 aggatgccgg tcgtcatcga gaaagaagat tacgatttgt ggctcgaccc agacatgaca
     1141 gatcctgtca aattggctga tctgctaaag tcgttcgact cgcgaaagat gcgcatttat
     1201 ccggttagtt cagccgtcaa cagcgtaaag aacgatgttc ccgagtgcgc agaagaggtg
     1261 actgtcgacg atgaaagctc atcgcagggc aacttgttct aacgtatgaa tggcataaaa
     1321 gtattctgca gagcggatgc cattttgctc cttccaagat tccgtatcgt gctgccccgt
     1381 cagtgagcat ccattgcttc cacgatctga cgagatagcc ttttggccgg cgtgagcagt
     1441 atcacggcat acctaaaatg aagttaagaa gaaatacgtt gatactttag gtattcatct
     1501 aatataccgg tcgactgctc acaatttagt gtccttagtt cgcaactaac atttttcgct
     1561 acgggtaccc atgaagtttg tctccttcct tgtcactttc ttttgtgcaa cagctctctt
     1621 cgcagttcaa ccacctcaat cggccacgac gccgctacct aatataaatt ttactagtgt
     1681 cggtactgta tcaagcgttc aattgactgg aaatgcaaat tgggtatttg gatccgatca
     1741 acagaccgga acagttactc ttcaagcgaa tgcagccggt cagagcagaa tgttgttgca
     1801 gctgtcgtcg gggccgcgtc ttgaaactca aaatgctttt tcggattcga ctagacaatg
     1861 cacttggagt ggtattgatg cagtcgttca caccagcgca gtacaccact gctgggtaga
     1921 tgcgatatgg tttcttcccc aaatcaccat gcaaataggt gcaggcgctt cagatgacag
     1981 cgcatcagca acggctgctc ccgacggaaa gattgtgcat attcaccacg aacggcatcc
     2041 cgtaggtgta cgggacgtcg gtacagccaa cttactggcg cacctcagtg cggttgacct
     2101 ggacatcgat gcaacgaccg gttttccttt ggaattaaac tttgcagccc atccagataa
     2161 tgacgcaggc accgacattt cggttcaggt gcaatttagt gattatcgct ctgtaaatgg
     2221 cgtgactata ccctttcata ttcagaaatt catcaacaac tcgctcgtcc ttgacctgca
     2281 aatttccac
//
LOCUS       DASRDH010000201         5425 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1213600,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000201 DASRDH010000000
VERSION     DASRDH010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 5425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5425
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1213600"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..732)
                     /locus_tag="VFA14_04575"
     CDS             complement(55..732)
                     /locus_tag="VFA14_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS80684.1"
                     /translation="MAELLRFPFENAHAIPKNFSQAWQDLFVLSMLDGRCNGRYLEIG
                     ANFARSNNNTCLLSSSFGWEGVSIEFDPQFLRSWQQERPDSNLVIADALALDYAQALP
                     RWFGADCKRLDYLQLDIDPSYNTLLALKRLPLDDWRFSVITFETDAYAGDLRARDESR
                     EIFSRHHYELVAADLCVLFVAAAPDPIPFEDWWVDPQVVSRNKIDTLKRLATQPLLPQ
                     HLLFDAG"
     gene            complement(836..1687)
                     /locus_tag="VFA14_04580"
     CDS             complement(836..1687)
                     /locus_tag="VFA14_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017449829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS80685.1"
                     /translation="MTAHIYQIFYDDATEKQLDRGFIPLANPGNPRADWREYWPIRNF
                     LLDNVLDEDDCYGFLSPAFANKSRLSSEKVFEFIRANPGQDVYSFSPLRQDSLCYLNI
                     FEHGNRFHPGMLEVINMFFDEIQLSANLASLVMDLQTTIFCNYLVAKPAFWQKWFALT
                     EKLFDIAEHETTPLSRSLNDQTMYRYSKNTAGMKVFIMERIASLLVSLDPELSVRAYD
                     IEQMPWSEITYYPYRDELMTLNALKLAYLHAGGQTYLRLFFEARNRILEKCDPMYPQE
                     RKQHFFD"
     gene            1948..2994
                     /locus_tag="VFA14_04585"
     CDS             1948..2994
                     /locus_tag="VFA14_04585"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017449866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZS80686.1"
                     /translation="MKILLLNDEFYTTGASIAMLRLAERLIQNHEVFVMPRVNGEGEI
                     KKKFEALGIPIVSSIQSIDLLVANTLMAGANVAEAAQKCPVVWWVHEAEIGRDILLRF
                     PRCASGFRQASHIVFQTGYQRKVYGSFLFDSPAMVHVLPFWNDAIYRQEELSPVTKVS
                     KRIVCIGTIDPRKRMEDTMMAVAALEPALREQIECVFIGKYIQLGEEARALVNAHPER
                     YRLLGELPNETALTWLASADAFVLASNSESQPLTIWEAFELEVPVLLSDLETYRHMGL
                     FHARHVMMHPVGNIEILSANLRLLLTSDTLRASLVRAGKSFLLNLLSSDWADEFERII
                     HRACARAEVKKLGY"
     gene            2930..4492
                     /locus_tag="VFA14_04590"
     CDS             2930..4492
                     /locus_tag="VFA14_04590"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008373 - sialyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 29 protein"
                     /protein_id="HZS80687.1"
                     /translation="MNSNASFIVPVRGPKSKSSVIEYISMQNSLVMFSTSEDLLKLLP
                     QPIAVVGNGIMKRRYGELIDSYATVIRMNNYRIQGREADVGRKTSLRCTTGWPDIEVR
                     NQYLEFTPFTNTAKESSHLSSYQNQGAAPVITANTDIHPLLPDTPHPSVELALLTLMA
                     QLGIGADVFGFNGGKTGNDWQVKTAGHKTPSSTEFDEILKLPNLRLFDDTHPDERPSD
                     SPHSEHNGRNINAGLALFKKLDLQIKNSRLIEFGAGNGDLSLYLQSLGNQVTAVEASV
                     IAFERIRIRDKIHGNCLDLPLIKKDFDWFISVGVLGNLMMNEINIVISEAARLAKGAL
                     VLISTRSGSLQADGKSQHEISWWEERFRAHFDTVTTIPMNLSDQFVLKASGSKRSHVR
                     APAIFKSYPSSGKDKAKAGDSFIKPGCQARGKAECIEMPQSALLDIGASKIDAGQHQA
                     AFKTLTSALEADPENAKLVFHLGRLAIACGMMEDAKELFTHAAARSPGMMNAIIDFYH
                     QQLQSARSPERQ"
     gene            4666..5211
                     /locus_tag="VFA14_04595"
     CDS             4666..5211
                     /locus_tag="VFA14_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HZS80688.1"
                     /translation="MPSMLDPIFVGTVVYLCEHNQNGALGVVINKPVGMTMDVLFDRI
                     DLKLNIVHDSSDARPDLSRTPVMFGGPVQVERGFVLHTGMNPFSSTLQVTDEISLTTS
                     KDVLEAVAHGDGPRQILVSLGCSGWSAGQLEEELVRNGWLTVKADPSIIFGLPVEQRF
                     TAALALLGIDPVMLSNDFGRA"
     gene            5222..>5425
                     /gene="ruvX"
                     /locus_tag="VFA14_04600"
     CDS             5222..>5425
                     /gene="ruvX"
                     /locus_tag="VFA14_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017454300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HZS80689.1"
                     /translation="METLLAFDFGLKRIGVAVGNTLIRQAQALTVISAATNDARFTAL
                     GKLIDEWAPARCIVGLPCHPDGSG"
BASE COUNT         1196 a         1410 c         1586 g         1233 t
ORIGIN      
        1 cgattgaacg agctcgccgg gcgggcctgg tttttcctgg ttctgaacag gttctcagcc
       61 tgcgtcgaac agcaggtgct gcggcagcag cggctgcgtg gccaggcgct tgagcgtgtc
      121 gatcttgttg cggctgacca cctgcggatc gacccaccag tcttcgaacg gaatcgggtc
      181 cggcgcggcc gcaacgaaca gcacgcacag gtcggcagcg accagctcat agtgatggcg
      241 cgagaagatc tcgcggcttt cgtcgcgtgc gcgcagatcg cccgcatagg catcggtttc
      301 aaatgtgatc accgagaagc gccagtcgtc cagcggcagg cgcttcaggg ccagcaaggt
      361 gttgtagctg gggtcgatgt ctaattgcag atagtcgaga cgcttgcagt cggcgccgaa
      421 ccagcgcggc agtgcctggg cataatccag cgctagggca tccgcgatga ccagattgct
      481 gtccgggcgt tcctgttgcc aggaacgcag gaactgtgga tcgaactcga tcgacactcc
      541 ctcccaacca aagctgctgc tgagcaggca ggtattgttg ttggaacgcg cgaagttggc
      601 gccgatttcc agatagcgcc cgttgcagcg tccgtcgagc atggacagca cgaacagatc
      661 ttgccaggcc tgcgagaaat ttttcgggat cgcatgcgcg ttttcaaatg ggaagcgtag
      721 cagctcggcc atggcgggct gccagttcca atggtgtgaa agggaattgc ggttcatcat
      781 tgccgtttgt cggttgcgcg cggatggcat ggctatgccg ccggcgggct gtggcctagt
      841 cgaagaaatg ctgcttgcgt tcttgcggat acatcgggtc gcacttctcg aggatgcggt
      901 tgcgcgcctc gaaaaagagc ctcaggtaag tctggccgcc ggcgtgcagg taagccagtt
      961 tgagtgcatt gagcgtcatc agttcgtccc gataggggta gtaggtgatt tcggaccagg
     1021 gcatctgctc gatgtcgtag gcccgtaccg acaattccgg atccagagag accagcagcg
     1081 atgcgatgcg ttccatgatg aataccttca tgccagccgt attcttgctg taacggtaca
     1141 tggtctggtc gttgaggctc ctggacagcg gagtggtttc atgttccgcg atgtcgaaca
     1201 gcttctcggt caaggcgaac catttttgcc agaatgccgg ttttgcgaca aggtagttgc
     1261 agaagatcgt cgtctgcaaa tccatgacca ggctcgccag gttcgccgag agctggattt
     1321 cgtcgaagaa catgttgatc acctcgagca tgccgggatg gaaacggttg ccatgttcga
     1381 agatattgag atagcacagg gaatcctggc gcagcggcga aaagctgtag acgtcctgtc
     1441 cgggattggc gcggatgaat tcaaatacct tctccgagga caggcgcgac ttgttggcga
     1501 aggccggcga gagaaaaccg tagcagtcat cctcatcgag cacgttgtcc agcaggaaat
     1561 tgcggatggg ccagtattca cgccaatccg cgcgcggatt gccgggattg gccaggggaa
     1621 tgaagcccct gtcgagctgt ttttcggtcg cgtcgtcgta gaagatctgg tagatatggg
     1681 cggtcatcag gagtccatgg ggtcaggccg tcaggaggcc cggaacctgt tgcactactt
     1741 gcttcagcca ggccggcagc tgagaacctg ttcagaattt tcttcgaaaa acgctcgtat
     1801 gactcccggc gagatcctgg gcagcttcta agacgaaagt gtaacatttc ttgttacttt
     1861 gttaagatgg cggctggttt atgttgcatg gaaacataag ctccggcgct atcgaatcga
     1921 aacagaaacg atttttgaag gcagtgcatg aagattcttc ttttgaatga tgagttttac
     1981 actaccggcg ccagtatcgc catgctgcgg ctggcggaac gcctgataca gaaccatgaa
     2041 gtgttcgtca tgccgcgcgt caacggcgag ggcgaaatca aaaagaaatt cgaggcgctc
     2101 ggcatcccca tcgtcagcag tatccagtcc atcgatctgc tcgtggccaa taccttgatg
     2161 gctggcgcga atgtcgccga ggcggcgcag aaatgtccag tggtgtggtg ggtccatgaa
     2221 gctgaaatcg gtcgcgatat attgctgcgt tttccgcggt gtgcgtcggg cttcaggcaa
     2281 gcttcgcata tcgtgtttca gactggatac cagcgcaagg tctacggcag ttttttgttc
     2341 gacagtcctg ccatggtgca tgttttgccg ttctggaacg acgccattta ccggcaggaa
     2401 gaactgtcgc cggtaacgaa agtgagcaag cgcatcgtct gtatcggcac catcgatccg
     2461 cgtaaacgca tggaagatac gatgatggct gtcgcagcgc tggagccggc cctgcgtgag
     2521 cagatcgaat gcgtcttcat cggtaagtat attcaattgg gcgaagaggc gagggcgctt
     2581 gtgaatgcgc atcccgagcg ttatcgcctg ctgggggagt tacccaatga aaccgcactg
     2641 acctggctgg cgtctgccga tgctttcgtg ctggctagca acagcgagtc gcaaccattg
     2701 acgatctggg aggcattcga gctggaagtg ccggtgctgt tgtcggacct ggaaacctat
     2761 cgtcacatgg gtctgtttca cgcgcgtcat gtcatgatgc atccggttgg caacattgaa
     2821 atactgtctg ccaatttgcg tctgctgctg accagcgata ccttgcgcgc cagccttgtc
     2881 agggcaggaa agagtttttt gttgaatctg ctttccagtg attgggcgga tgaattcgaa
     2941 cgcatcattc atcgtgcctg tgcgcgggcc gaagtcaaaa agctcggtta ttgagtacat
     3001 atccatgcaa aacagccttg tcatgttcag cacgtcggaa gatctcttga aattacttcc
     3061 ccagccaatc gcagtcgttg gtaatggcat catgaagagg cggtatggag agttgatcga
     3121 ttcctatgcg acggtcattc gcatgaataa ctacaggata cagggacgcg aagccgatgt
     3181 gggtcgcaag accagcttgc gctgcaccac aggatggccg gatatcgaag tgagaaatca
     3241 atatctcgaa ttcacgccgt ttaccaatac cgcaaaggag tcctcccatc tgtcttcata
     3301 ccagaatcag ggggccgcgc cggtcatcac cgccaatacg gatattcatc cgctgctccc
     3361 ggatactccc catccttccg tggaactggc cttgctcaca ttgatggcgc aacttggcat
     3421 cggggcagat gtatttggtt tcaatggagg taaaaccggc aatgactggc aagtcaagac
     3481 tgccggccat aaaacccctt catcgacgga gttcgatgag atcctgaaat tgccgaatct
     3541 caggttattc gacgataccc accccgatga gcgacccagc gattcccctc acagtgaaca
     3601 caacgggcgc aacatcaatg caggcttggc gctgttcaaa aaactcgatc tgcaaatcaa
     3661 aaactccaga ctgatcgagt tcggagccgg caatggcgat ctttccctgt atctgcaaag
     3721 cctgggcaat caggtcacgg cagttgaagc ttctgtgatt gcttttgagc gcatacggat
     3781 ccgggacaaa atacatggta attgtcttga tttaccgctg atcaaaaagg atttcgactg
     3841 gtttatctct gtcggtgttc tgggaaatct gatgatgaac gagatcaaca tcgtcatcag
     3901 cgaggctgcc cgattggcaa agggcgcact ggttttgatt tcgacccgca gcgggtcact
     3961 gcaagccgat ggcaagagtc agcatgaaat cagctggtgg gaggaacggt ttcgcgcgca
     4021 tttcgatact gttacgacga ttccaatgaa tctgtcagat cagtttgtat taaaggcgtc
     4081 gggcagtaaa agatctcacg tgcgtgcgcc ggctattttt aaaagctatc caagcagcgg
     4141 taaagacaag gcaaaagccg gtgactcttt tatcaagcct ggttgtcagg cccgtggcaa
     4201 agcagaatgc atcgagatgc cgcaatctgc attgctggat attggcgctt cgaaaatcga
     4261 cgcggggcaa catcaggctg cattcaaaac attgacctcc gctttggaag cggacccgga
     4321 aaacgcaaag ctcgtattcc atctgggacg cctggcaatt gcctgcggga tgatggagga
     4381 cgcgaaagag ttgttcaccc atgctgcagc ccgcagtcca ggcatgatga atgccatcat
     4441 tgatttttat caccagcagc tgcaaagcgc gcgttcgccg gaacggcaat gaccggtcgt
     4501 aaaactgcta aaatgtcgga catgatcaag cgacacaaag cctcttccaa cgacactacc
     4561 gatgttgcct ccgggacgtc agcagattcc ggatttactg ccggcgccga tgaaaatctc
     4621 aaactccggc ccgggttcaa cctcaccgat catttcctga ttgccatgcc gtcgatgctg
     4681 gatccgattt ttgtcggcac cgtggtttat ctgtgcgaac acaaccagaa cggcgcgctt
     4741 ggcgtcgtca tcaacaaacc agtcggcatg acgatggatg tcctgttcga tcgcatcgat
     4801 ctcaaactga acatcgtcca tgactcgtct gacgccaggc cggatctgtc ccgcactccg
     4861 gtgatgttcg gcgggccggt gcaggtcgag cgtggctttg tgctgcacac gggaatgaac
     4921 ccgttttcct cgacgctgca agtgactgac gagatttccc tgacgacttc caaggatgtg
     4981 ctggaggcag ttgctcatgg cgacggtccg cggcagatac tggtcagtct gggctgctcc
     5041 ggctggagtg ctggtcagct ggaagaagaa ttggtgcgta acggctggct gaccgtcaag
     5101 gccgatccct ccatcatttt cggtttgccg gtggagcagc gcttcaccgc tgcgctggct
     5161 ttgctgggca tcgatccagt catgctcagc aatgacttcg gccgggcctg agcaggaatc
     5221 cgtggaaacg ctcctggcct tcgatttcgg cttgaaacgt atcggtgtgg cagtcggcaa
     5281 tactctgatc aggcaggcgc aggcgttgac cgtcatcagt gccgccacta acgatgccag
     5341 attcactgcg cttggtaaac tgatcgatga gtgggcaccg gcgcgctgca tcgtcggctt
     5401 gccctgtcat cccgatggca gcggg
//
LOCUS       DASRDH010000202         2544 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_378866,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000202 DASRDH010000000
VERSION     DASRDH010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378866"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="VFA14_04605"
     CDS             <1..436
                     /locus_tag="VFA14_04605"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013234934.1"
                     /note="catalyzes the oxidation of malate to oxaloacetate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HZS80690.1"
                     /translation="VWGNHSPTMYADYRFATANGKLVKDLINDQVWNIDTFLPTVGKR
                     GGAIIEARGLSSAASAANAAIDHVRDWILGTNGKWTTMGVPSDGSYGIPEGIMFGFPV
                     TTGNGEYQIVHGLDIDTFSQERINITLKELLEEREGVKHLVG"
     gene            576..1043
                     /locus_tag="VFA14_04610"
     CDS             576..1043
                     /locus_tag="VFA14_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS80691.1"
                     /translation="MKKDFFVLILVSALFFFSIASAFAEKKAPVKTTAPAKKMPFSSD
                     AEDDEREPEISGTSSTDFHCEMGQKLTIYIKDADDRHIAIRWNKRIHRLTRVTTTTGV
                     NRLENRKVGLVWIGIPAKSILLDSKKGRQLANECRNIESVMTGALAISQNPGR"
     gene            1180..>2544
                     /locus_tag="VFA14_04615"
     CDS             1180..>2544
                     /locus_tag="VFA14_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HZS80692.1"
                     /translation="MSRNTLNTLKEFEISTSRKGKFYSLSALEKKLGVNLSRLPVSIR
                     IVLESVLRNCDGQKVTEEHVRQLANWDAKAARVDEIPFVVARVVLQDFTGVPLLADLA
                     AMRNVASKLGRNPKNIEPLVPVDLVVDHSVQIDHYREKKALDLNMKLEFQRNNERYQF
                     MKWGMQAFDTFGVVPPGFGIVHQVNLEYLARGVHHKAGVYYPDTLVGTDSHTTMINGI
                     GVVGWGVGGIEAEAGMLGQPVYFLTPDVVGVNLTGALREGVTATDLVLTITELLRKTK
                     VVGKFVEFFGEGARSLSLTDRATIANMAPEYGATMGFFPVDEATIDYFKGTGRTKAEI
                     DAFEAYFKAQYLFGIPKAGDIDYTSEVSLDLGTVAPSLAGPKRPQDRIEIGNVKSTFA
                     DLFVKPLAENGFNKKIEDLTKTYTNADGVDIRNGDVLIAAITSCTNTSNPGVMLAAGL
                     LAKKA"
BASE COUNT          610 a          695 c          678 g          561 t
ORIGIN      
        1 tgtctggggc aaccactcgc caaccatgta tgccgactac cgctttgcta ccgccaacgg
       61 caagctggtc aaggacttga tcaacgacca ggtctggaat atagacactt tcctgccaac
      121 tgtcggcaag cgcggcggcg ccatcatcga agcacgcgga ttgtcctcgg ccgcatcggc
      181 agccaacgca gcgatcgacc acgtgcgtga ctggatcctc ggcaccaatg gcaaatggac
      241 taccatgggc gtgccttcgg acggttccta tggtatcccg gaaggtatca tgttcggctt
      301 cccagtgacg actgggaacg gtgaatatca aatcgtccac ggcctcgaca tcgatacgtt
      361 ctcgcaagag cgcatcaaca tcacgctcaa ggaattgctg gaagaacgcg aaggcgttaa
      421 gcatctggtc ggctgattga cctgcgcgcg gcggtggtat cgccgcgcat gttgagtacc
      481 tcctcatgca tcctacccag ggcttgttcc cagacactcg acaatcggtc tggttacggt
      541 ctgccatcat gaatattgat atcgatcgca gcatgatgaa aaaagatttt tttgtcctga
      601 tactggtaag cgctcttttc ttcttcagca tagcgtctgc gttcgcggaa aagaaagcgc
      661 ctgtcaaaac tactgcgcca gcaaaaaaaa tgccattttc ttcggatgct gaagacgacg
      721 aacgcgaacc ggaaatcagc ggcaccagct caactgactt ccactgtgaa atggggcaga
      781 aactgaccat ttatataaaa gatgctgacg acaggcacat tgcgattcgc tggaataagc
      841 gtatccatcg cctgacacgt gttactacta ctaccggcgt caaccgtctc gaaaaccgca
      901 aggtcggcct ggtctggatc ggcattccag ccaagagcat cctgctggat tccaagaaag
      961 gccggcaact ggcgaacgaa tgcaggaaca tcgaatcggt catgacagga gcgctggcaa
     1021 tttcacaaaa cccgggccgg taacacgtat ccgacatcat tggcattcga cagtagcgta
     1081 gttctgcatt tgctgtgcaa caccactcaa cacggttaaa tactattttg tggtattcac
     1141 aaaccattca tactacttgt catctaagga gaagaggcca tgtctcgtaa cacattgaac
     1201 accctgaagg aattcgagat ttctacttcc aggaaaggca agttctactc tctgtccgcc
     1261 ctggaaaaga aacttggggt caatctctcc cgtttgccgg tatcgatccg catcgtcctg
     1321 gaatcagttc tgcgtaactg cgatggtcaa aaagtgactg aagaacatgt caggcaactc
     1381 gccaactggg atgccaaggc cgctcgcgtc gacgagatcc cattcgtcgt agcccgcgtc
     1441 gtgctgcaag atttcaccgg cgtgccgctg ctggctgact tggctgcgat gcgtaacgtc
     1501 gccagcaagt tgggcaggaa tccaaaaaat atcgaaccgt tggtaccggt agatctggtg
     1561 gtcgaccatt ccgtgcagat tgaccattac cgtgaaaaaa aagcactgga cctgaacatg
     1621 aagctggaat ttcagcgtaa caatgagcgc taccagttca tgaagtgggg catgcaggca
     1681 tttgacactt tcggtgtcgt gccgccgggc ttcggtatcg tccaccaggt caatctggaa
     1741 tacctggcgc gtggtgtaca ccacaaagcc ggtgtctatt accctgacac cctggttggc
     1801 accgattcgc acaccaccat gatcaatggc attggtgtgg tcggctgggg cgtgggtggc
     1861 atcgaggcgg aagctggcat gctgggccag ccggtttact tcctgacgcc ggacgtggtt
     1921 ggcgtcaatt tgaccggcgc gctgcgtgaa ggcgtcaccg cgaccgatct ggtgctgacc
     1981 attactgaac tgttgcgtaa aaccaaggtc gtgggcaagt tcgtcgagtt ctttggcgag
     2041 ggcgccagat cgctgtcgct gaccgaccgc gccactatcg ccaacatggc gcctgagtac
     2101 ggcgcgacca tgggtttctt cccggtcgat gaagcaacca tcgattactt caagggcacc
     2161 ggccgcacca aagcggaaat cgatgcattc gaggcttatt tcaaggcaca gtacttgttc
     2221 ggcattccca aggccggcga cattgattac accagcgaag tgtcgctgga tctgggcacc
     2281 gttgcaccgt cgctggctgg tccgaagcgt ccgcaagacc gtatcgaaat cggcaatgtc
     2341 aagtcgactt tcgccgatct gtttgtcaag ccgcttgctg aaaacggctt caataagaag
     2401 atcgaagatc ttaccaaaac ctatactaat gccgacggcg tcgatatcag gaacggcgac
     2461 gtgttgattg ctgcgatcac ttcctgcacc aatacctcga acccaggcgt gatgctggct
     2521 gctggtctgc tggccaagaa agcg
//
LOCUS       DASRDH010000203         3258 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_305596,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000203 DASRDH010000000
VERSION     DASRDH010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3258
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305596"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="VFA14_04620"
     CDS             complement(<1..510)
                     /locus_tag="VFA14_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HZS80693.1"
                     /translation="MDKQNIPMQSVQAQLGDLGATLDPAEVLRLQKDYADQFTSLWHI
                     VVKAGVPPISDRRMSTPVWKASPFFAFQAALYQLNSRFLMALVDAVQSNSKVKRKLRF
                     AAQQMVDALSPANFLATNPEAQQKLLDTRGESLTRGMAQLFADLQKGHISQTDETAFE
                     IGKDVATTEG"
     gene            574..786
                     /locus_tag="VFA14_04625"
     CDS             574..786
                     /locus_tag="VFA14_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS80694.1"
                     /translation="MHIIAIAWLYVIFMISITEQSAIAGIISFFLYGVFPLTIVLYLI
                     RTPQRKRNRHHQIKSGTRVENKNGRS"
     gene            complement(800..1576)
                     /gene="pgeF"
                     /locus_tag="VFA14_04630"
     CDS             complement(800..1576)
                     /gene="pgeF"
                     /locus_tag="VFA14_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HZS80695.1"
                     /translation="MELLIPEWSDRPANVGALTTLRQGGVSQGPYGDGCDRGGLNLAA
                     HVQDDPVHVVRNRHVLRSILPSEPLWLRQVHGVDVVDAVEAAGAAEAPQADACVTTQA
                     GVVCAVMTADCLPVLFCDLAGTVVGAAHAGWRGLAAGVLEHAVAAMRDRGAGEILAWL
                     GPAIGPQQFEVGQEVLDAFVAQDAASRAAFSASVGREGKFLADIYRLARLRLAQVGVR
                     RVSGGDWCTVSDTRFYSYRRDEVTGRMASLIWFRQPVLAG"
     gene            complement(1590..2636)
                     /locus_tag="VFA14_04635"
     CDS             complement(1590..2636)
                     /locus_tag="VFA14_04635"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HZS80696.1"
                     /translation="MSHTTPPGFVKTCAADLADAGALDEVNDEVDEENSPIVLKLSAM
                     DGGSRLDKVLSTLVPQYSRSRIQQWIEAGHVTVDGKSARARMTVLGDEVVIMTPQAAP
                     EENAFQPEAMALEIVYEDEAILVLDKPAGLVVHPAAGNWSGTLLNGLLHYCPTLAGVP
                     RAGIAHRLDKDTSGLMVVAKTLEAQTDLVRQLQARTVRRRYLALVWGLPQLSGIVNAA
                     MARHPRDRIRMAVSESVTAKPAITHYQRLAVGALDGRVASLVHCQLETGRTHQIRVHM
                     QSLGFALVGDTLYGRAHLMPFFPRQALHAWRLGLVHPVSRRQREWEAGLPQDFAELLE
                     RAEIDVGLLGAIGK"
     gene            2699..>3258
                     /locus_tag="VFA14_04640"
     CDS             2699..>3258
                     /locus_tag="VFA14_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HZS80697.1"
                     /translation="MQKILSSLLTAALILSLSACGLLSEQKDEMADWSVSKLYSEAKD
                     ELAAGSYEKAIKYFEKLESRYPFGAYAQQAQMNIAYAYYRLNDQPQGLAAVDRFIKLY
                     PNHPHLDYIYYLRGLINFDGRTMIFDTITDQDSTERDPKAIRDAFDSFKLLVERFPDS
                     TYTPDATLRMKYLVNAMAQYDVHVAR"
BASE COUNT          719 a         1056 c          851 g          632 t
ORIGIN      
        1 accttcggtc gtcgccacat ccttgccgat ctcgaacgca gtttcatcgg tttgcgaaat
       61 atggcctttc tgcagatcgg caaacaattg cgccatgccg cgcgtcaggc tctcccctct
      121 ggtatcgagc agcttttgct gggcttcagg attggtagcc agaaagttcg ctggcgacag
      181 ggcatcgacc atttgttgcg ctgcgaaacg cagtttccgc ttgaccttgc tgttcgactg
      241 aacagcatcg accaaggcca tcaaaaaccg tgagttgagc tggtaaagcg ccgcctgaaa
      301 tgcaaaaaac ggactggctt tccagactgg cgtcgacatg cgccggtcgg aaatcggcgg
      361 caccccggcc ttgaccacga tgtgccacaa agaggtaaac tgatcagcgt aatctttttg
      421 caaccggagt acttcggcgg ggtccagagt cgcccccaga tcgcccaatt gcgcctgcac
      481 cgattgcata gggatattct gcttatccat gctcgcttct tatatctttc gcggttacag
      541 tttgccgccc agactgtaaa aatggagggc gcaatgcaca tcatcgccat cgcctggctc
      601 tatgtcatct tcatgatatc gatcaccgaa cagtccgcca tcgcaggaat catcagcttt
      661 tttctctatg gtgtatttcc gttgactatc gttttatacc tgatcaggac gccgcagcgc
      721 aagcggaatc ggcatcatca aataaaaagc ggcacacgcg tcgaaaacaa aaacgggagg
      781 tcgtgaacag gttccaggct catcctgcaa gcactggttg gcgaaaccag atcagcgaag
      841 ccatgcgtcc cgtgacctcg tcgcgacggt aggaataaaa acgcgtatcg ctgaccgtac
      901 accagtcgcc gcccgatacg cgccgtacac cgacctgcgc caggcgcaaa cgggcaagcc
      961 ggtaaatatc cgcaaggaac ttaccctcgc gcccaacact cgcggaaaag gcagcgcggc
     1021 tggcggcatc ctgtgcgacg aaagcgtcca gcacctcctg cccgacctca aattgctgcg
     1081 gcccgatggc ggggccgagc catgccagaa tttcgccggc gccgcgatca cgcattgccg
     1141 ccacagcatg ctccagcaca ccggcggcca aaccgcgcca tccggcatgc gccgcaccaa
     1201 ccaccgtgcc ggccaggtca cagaacagca ccggcaaaca gtccgctgtc atgactgcgc
     1261 acacgacacc ggcctgcgtc gtcacgcagg catcagcctg cggcgcctct gcggcacccg
     1321 ctgcttcgac agcatcgact acgtcgacgc catgcacctg cctcagccag agcggctccg
     1381 acggcaggat actgcgcaat acatggcgat tgcgcaccac atgtaccgga tcatcctgca
     1441 catgcgcggc caggttcaag ccgccacggt cgcagccatc accatacggc ccctggctga
     1501 cgccgccctg gcgcaaagtc gtcagcgcgc caacattggc aggacggtcg ctccattcag
     1561 ggatcagcaa ttccatcgta tccgcccgtt tatttcccga tggcgccgag aaggccgaca
     1621 tcgatctctg cacgctccag cagttcggcg aaatcctgtg gcaaaccggc ttcccattcg
     1681 cgctgcctgc ggctgaccgg atgcaccaga ccaagccgcc aggcgtgcag cgcctgacgc
     1741 gggaaaaacg gcatcagatg cgccctgccg tacaaagtat cgccaaccag cgcaaaaccg
     1801 agcgactgca tatgcacgcg aatctgatgc gtgcgccctg tttccagctg gcaatgcacc
     1861 aaactggcca cgcgtccgtc cagcgccccc acggcaagac gttgataatg ggtgatcgcc
     1921 ggcttggcgg tcacgctttc cgataccgcc atcctgatcc ggtcacgcgg atgacgtgcc
     1981 atggcagcat tcacgatgcc agacaattgc ggcagccccc agaccagcgc cagataacgg
     2041 cgcctgactg tccgcgcctg caactgccgc accagatcgg tctgcgcctc cagcgtcttg
     2101 gccaccacca tcaaaccgct ggtgtccttg tcaagccgat gcgcgatgcc ggcgcgcggc
     2161 acgccggcca gcgtcgggca ataatgcagc aagccgttca acaaggttcc cgaccagttg
     2221 cccgcagccg gatgaaccac cagtccggcg ggcttgtcga gtaccagaat agcttcgtcc
     2281 tcatacacga tctccaacgc catggcctcg ggctggaagg cattttcctc cggtgcggcc
     2341 tggggcgtca tgatgacgac ttcatcgccc aacaccgtca ttctggcgcg tgcagacttg
     2401 ccgtccacgg tcacatgacc ggcttcgatc cattgctgga tacggctgcg cgagtattgc
     2461 ggaaccagtg tcgagagcac cttgtccagc ctgctacctc catccatggc gctcagtttc
     2521 aggacaatcg ggctgttttc ttcgtcaact tcgtcgttga cttcgtcgag cgcacctgca
     2581 tcagcaagat cggctgcgca tgtttttaca aacccaggtg gagtagtatg tgacatcagt
     2641 tataatcagc ggcgtaaaat ccctgtttct ttgcacacgc ccaaactgct catacgtcat
     2701 gcaaaaaatc ttgtcgagcc tgcttactgc cgcactcatc ctttccctgt cggcgtgcgg
     2761 tctgctgtcc gaacaaaaag acgaaatggc ggattggtcc gtgtcgaaat tatactcgga
     2821 agccaaggac gaactggctg cgggcagtta cgagaaggcc atcaagtact tcgagaaact
     2881 tgaatcgcgc tatcccttcg gcgcctatgc gcaacaggcg cagatgaaca tcgcctacgc
     2941 ctactaccgc ctgaacgacc agccgcaagg cctggcagca gtggaccggt tcatcaagtt
     3001 gtatccgaac cacccccatc tcgactatat atattacctg cgcggattga tcaatttcga
     3061 tggccgcacg atgatcttcg acaccatcac cgaccaggac agtaccgaac gcgatcccaa
     3121 agcgatacgc gacgctttcg actcgttcaa actgctggtg gaacgctttc cggacagcac
     3181 ctacacaccg gacgccacac tgcgcatgaa gtatctggtc aatgcgatgg cgcaatacga
     3241 tgttcacgtc gccaggta
//
LOCUS       DASRDH010000204         5152 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_696397,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000204 DASRDH010000000
VERSION     DASRDH010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 5152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5152
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_696397"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..735)
                     /locus_tag="VFA14_04645"
     CDS             complement(<1..735)
                     /locus_tag="VFA14_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HZS80698.1"
                     /translation="MKLKYLIIAIGLLFAALSQAADMKELRIGMEPLYKPFEYRTPDG
                     RLVGFDVDIANALCAEMKMKCVFIAQSFDGLIPALMAKKYDVIISSMTRTEERRKAVA
                     FSEKIYNTPSTIITRKGSSIDVSPASLKGKKVGVLKGSAQEAYAVKVLRPAGATVLSY
                     ANTPDSYLDMESARIDAVVADLVEVRSGLLDKPQGKDFAIAVSGLVDPVFANGTGAAV
                     RKQDRQLLEQINAAIKAIRANGTYQKI"
     gene            complement(870..2210)
                     /gene="astB"
                     /locus_tag="VFA14_04650"
     CDS             complement(870..2210)
                     /gene="astB"
                     /locus_tag="VFA14_04650"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005664725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HZS80699.1"
                     /translation="MHTAREFNFDGLVGPSHNYAGLSFGNVASFNNVKSVSNPRQAAL
                     QGLTKMRALAARGFAQALLPPQIRPCFSLLRVLGFSGSDAQVIARAWRDAPALLSCAY
                     SASPMWTANAATVSPAADTADGRTHFTVANLNSKLHRSYEHTQTERALRALFRDERYF
                     AVHGALPATPAFGDEGAANHTRLCAAHGAAGVEMFVYGRVEFDPQAAAPQRFPARQTL
                     EASQAVARLHGLQARRTVYVQQHPAVIDQGVFHNDVIAVGNGNVLFYHQQAFRDESAS
                     LSALRQAMAETGAELQTVRVDTAEVSVADAVSSYLFNSQLLSRSDGKMLLVVPQECQE
                     NPAVARYLSGLLDGGGAIDELIHFDLRQSMRNGGGPACLRLRVALNEAEAAAMHQGVI
                     MTEALYTRLVAWVEKYYRDRLAPEDLADPLLAIEVRTAMEALSRILDLPDLYHF"
     gene            complement(2240..3703)
                     /gene="astD"
                     /locus_tag="VFA14_04655"
     CDS             complement(2240..3703)
                     /gene="astD"
                     /locus_tag="VFA14_04655"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HZS80700.1"
                     /translation="MHSHYIDGQWIVGAGPELVVFDPASGRRIWTGTAATAAEIDRAC
                     QAARASFGAWAKTGLDERIAICARFRDLLKAHGEELARCIAQEVGKPLWEARTEVTSM
                     AGKIDISVQSYRVRTGETSGSIADGVTVLRHRPHGVFGVFGPYNFPGHLPNGHIVPAL
                     IAGNTLVFKPSESAPQTAIRTVELWAQAGLPDGVINLVNGGRDTGAALARHAALDGIL
                     FTGSYRTGAILHQQYAGWPGKMLALEMGGNNALVVWNVSNLDAAVHHTIFSAFVSAGQ
                     RCTCARRLIIEDSAQGQVFLQRLVAVAGNIRVGAFDQSPPPFMGPVVSAATAKRLLQA
                     QADMLERGGKALLAMRHLQAGTGLLSAGIIDVTEASGIADEEWFGPLLQVVRVAAFDA
                     AIDVANDTQYGLACALLSDDAALWQQFLLRARAGVVNWNRPTTGAASTAPFGGVGKSG
                     NHRPSAYYAADYCAYPVASIENSVLDMPQQLSPGLVF"
     gene            complement(3696..4733)
                     /gene="astA"
                     /locus_tag="VFA14_04660"
     CDS             complement(3696..4733)
                     /gene="astA"
                     /locus_tag="VFA14_04660"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HZS80701.1"
                     /translation="MLLVRAVKEDDLDGLMELSTQAGSGMTTLKADRGMLGHRVEVAC
                     ASFAEKIAPPQRDYLFVMEDLVAGRLVGVSAIKSAVGLDEPFYNYRIGTLVHASKELK
                     VFSRMDTLYLSNDLTGSSELCSLFLHPAYRSGSNGKLLSKCRFLFMAQFPHLFSRKII
                     AEMRGFQRADGTSPFWDSLGQHFFRMDFNRADDLSGLGKKSFIAELMPRHPLYVAYLP
                     PEAQEVIGRVHADTAPARRLLEQEGMYYEGYVDIFDAGPVLQARVSELRAVREGALAT
                     VRLAAPDTAADHPVLVANTVLHDFRIIAPHAHAGQEYIALTPEHQRLLDCRCGDPVRT
                     MSLTPQKNTYA"
     gene            complement(4742..>5152)
                     /locus_tag="VFA14_04665"
     CDS             complement(4742..>5152)
                     /locus_tag="VFA14_04665"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: protein motif:HMM:NF016822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HZS80702.1"
                     /translation="MMPHYPVYVPLLPGEAQTAMGQAHGAGELPFRILSQEGFEPDEF
                     IDIFDGGPILQAHRNSLRSFSASTTHQLAGASVAVRDVSPLPYLVSNTREERFRAVVT
                     DLAPLERSDELSLPPEVLRALDVVAGDSVSCVKL"
BASE COUNT         1158 a         1609 c         1393 g          992 t
ORIGIN      
        1 aattttctga taagtaccgt ttgcgcgtat cgccttgatt gctgcattga tctgctccag
       61 cagttgacga tcctgtttgc gcaccgccgc gccagttccg ttggcgaaga cgggatcgac
      121 cagaccggaa acggcgatag caaaatcttt tccttgtggc ttgtccagca gaccagagcg
      181 gacttccacc agatccgcca ccacggcgtc gatacgagcg gactccatat ccagataaga
      241 atccggcgta ttcgcatagc tgagcaccgt cgcgccggcc ggcctcaata ctttcacggc
      301 ataagcttcc tgcgccgagc ctttcaatac accgactttt tttcctttga gtgaggctgg
      361 cgacacatcg atgctactac cttttctggt gatgatagtc gatggcgtgt tatagatttt
      421 ttcggaaaac gccactgctt ttctgcgctc ttcggtgcgc gtcatagaag agatgatgac
      481 gtcgtatttt tttgccatca gcgcaggaat gagaccatcg aatgattgcg caatgaacac
      541 gcatttcatt ttcatctcag cgcacaaggc attggcgata tcgacatcga accccaccag
      601 ccgtccatca ggcgttctgt actcgaacgg tttataaagc ggctccatgc cgatacgcag
      661 ctctttcata tcagccgcct gggataaagc cgcgaacagc aggccgatgg caatgatcaa
      721 atatttcagt ttcatgaacg tgtctccaga ttatagatat cgatttttcc tacacaccgc
      781 taaagcaaat atcgtgccag gacagaaaat atgtcgaaac agcgacagag aatatcccat
      841 ttcagtggcg cagttcaatt gctacaagat caaaaatggt ataaatccgg taaatccaga
      901 atacgactga gcgcctccat ggcagtccgc acctcgatgg ccagcaacgg gtcggccaaa
      961 tcttccggcg ccagacgatc acggtaatat ttttctaccc aggccaccag ccgggtatac
     1021 aaagcttccg tcatgatcac gccctgatgc atcgccgcgg cctcagcctc gttgagcgct
     1081 acccgcaggc gcaaacaggc cgggccgccg ccattgcgca tgctttgccg caaatcgaaa
     1141 tgaatcaact catcgatcgc gccaccgccg tccagcagtc ccgacagata gcgcgctacc
     1201 gcagggtttt cctggcactc ctgcggcact accagcaaca tcttcccatc cgacctggaa
     1261 agcagttggc tattgaacag gtaactggaa acggcatccg ctaccgatac ctcggcagta
     1321 tcgacccgca ccgtctgcaa ctctgcgccg gtttccgcca tggcttgccg caatgccgat
     1381 aggctggccg attcatcgcg gaatgcctgc tggtgataaa acaggacatt gccgttaccg
     1441 acggcaatca cgtcattatg gaatacgccc tgatcgatga ccgcaggatg ctgctgaaca
     1501 tatacggtgc gccgcgcctg caaaccgtgc aggcgcgcca cggcctggct ggcctccagt
     1561 gtctggcgcg ccggaaaacg ctgcggcgct gctgcctgcg gatcgaattc tacccgacca
     1621 tagacgaaca tctctacccc ggcagcaccg tgcgcagcgc agagccgagt gtgatttgcc
     1681 gcgccttcat cgccgaacgc cggcgtcgcc ggaagcgcgc catgcacggc aaaataccgc
     1741 tcatccctga acagcgcacg caatgcgcgt tccgtctgcg tatgctcata tgagcgatgc
     1801 agtttgctgt tgaggttggc aacggtgaag tgggtgcggc catcggctgt gtcggcagca
     1861 gggctgacag tggccgcatt cgccgtccac atcggtgagg ccgaataagc gcaagacagc
     1921 agcgccggcg catcgcgcca ggcgcgcgcg atcacctgcg catcgctgcc gctgaaaccg
     1981 agcacgcgca gcaaggagaa acagggccgg atctgcggcg gcaacagggc ctgggcaaag
     2041 ccgcgcgccg ccagggcgcg cattttggtc aagccctgca gcgccgcctg cctgggattg
     2101 gagacactct tgacgttatt aaacgaagcc acattgccga aagaaaggcc agcgtagtta
     2161 tgcgatgggc cgaccaggcc atcgaaattg aattcacgtg cggtatgcat catggttacc
     2221 attgggtatc tgcgccaagc taaaacacca gaccaggtga aagttgttgc ggcatgtcga
     2281 gcacgctgtt ttcaatcgac gcgaccggat aggcgcaata gtccgctgcg tagtaggcgc
     2341 tgggacgatg attgccggat ttacccacac caccgaatgg cgcggtgctg gcagcgccgg
     2401 tcgtgggccg gttccagttg accacaccgg cgcgtgcgcg caacagaaac tgttgccaga
     2461 gcgccgcatc atcgctcagc aaagcacagg ccagaccgta ttgcgtgtca ttggcgacat
     2521 cgattgccgc gtcgaaagcg gcgacccgca ccacttgcag caatggtcca aaccactctt
     2581 catcggcaat cccgctggcc tcggtgacat cgatgatacc cgccgacagt aaaccggtcc
     2641 cagcttgcag atgacgcatg gcgagcaagg ctttgccgcc ccgctccagc atatcggcct
     2701 gggcctgcag cagccgtttt gcagtggcag cggacaccac cggccccatg aaaggcggcg
     2761 gcgactgatc gaacgcaccc acacggatat tgcccgcgac cgcgaccaga cgctgaagaa
     2821 agacctgtcc ctgcgcggaa tcttcgataa tcaggcgacg ggcgcaggtg cagcgctgac
     2881 cggcgctgac aaaggcagaa aaaatggtgt gatgcacggc ggcatccaga ttgctcacat
     2941 tccacaccac cagcgcattg ttgccgccca tttcgagcgc cagcatcttg cccggccagc
     3001 cggcatactg ctggtgcaag atcgccccgg tccggtagct gccggtgaac aagataccgt
     3061 ccaacgcggc gtgtcgcgcg agtgcggcgc cagtgtcgcg gccaccattg accagattga
     3121 tgacaccatc aggcaagccg gcctgcgccc acaattcgac cgtcctgatc gcagtctgcg
     3181 gcgcagactc gctcggtttg aagaccagcg tattccccgc aatcagcgcc ggcacgatat
     3241 gcccattcgg cagatgaccg ggaaaattgt acggaccgaa cacgccgaac acgccatgcg
     3301 gacgatgccg caaaacggtg acgccgtcag cgatgctgcc cgacgtttca ccagtgcgga
     3361 cacgatagga ctgcaccgaa atatcgatct tgccagccat cgacgttact tcagtacgcg
     3421 cttcccacag gggttttcct acctcttgcg caatgcatcg cgccagttct tcgccatgtg
     3481 ctttcagcag atcacgaaag cgcgcgcaaa tggcaatccg ctcatcgagt ccggttttcg
     3541 cccaggcgcc gaagctggcc cgtgctgcct ggcaggcccg atcgatttcg gccgcagtcg
     3601 ccgccgttcc tgtccagatc cggcgtccac tggcgggatc gaaaacaacc agttccgggc
     3661 ctgcgcccac gatccattga ccgtcaatat aatggctatg cataagtatt cttttgggga
     3721 gtgagcgaca tggtgcgtac cggatcgccg caacggcaat ccagcaagcg ttgatgctca
     3781 ggagtcaatg cgatgtattc ctgtcccgca tgcgcatgcg gcgcaatgat acggaaatca
     3841 tgcagcaccg tattagcgac caaaaccggg tgatcggcgg cggtatccgg cgctgccagc
     3901 cggacagttg ccaatgcgcc ttcacgcact gcccgcagtt cgctcacgcg cgcctgcagc
     3961 accggcccgg catcgaaaat atcgacatat ccttcgtaat acatcccttc ctgttccagc
     4021 agacggcgcg ccggcgcggt atccgcatgc acccggccga tgacttcctg tgcctcgggc
     4081 ggcaggtagg ccacatatag cggatgtcgc ggcatcagtt ccgcgataaa ggattttttc
     4141 cccaggccgc tcaaatcgtc ggcacggttg aaatccatcc tgaaaaaatg ctggcccaaa
     4201 ctatcccaga acggcgaagt cccatcggca cgctgaaagc cacgcatctc ggcaatgatt
     4261 ttccgggaaa acaaatgcgg aaactgcgcc atgaaaagaa accggcactt cgacagtaac
     4321 ttgccattgc tgccgctgcg ataggcagga tgtaaaaaca gcgaacataa ttcgctgctg
     4381 ccagtcagat cgtttgacag gtagagcgtg tccatgcggg aaaacacctt cagttccttg
     4441 ctcgcatgca ccagggttcc gatccggtag ttataaaacg gctcatccag tccgaccgcg
     4501 ctcttgatcg cgctcacacc gaccaggcgg ccagcaacca gatcttccat cacgaacaga
     4561 tagtcgcgct gcggcggcgc aattttttct gcaaatgaag cgcacgcgac ttccacccga
     4621 tgaccgagca tcccgcgatc cgccttcagg gtcgtcatgc cgcttcctgc ctgcgtggaa
     4681 agctccatca ggccatccag atcgtcctct ttgacagcgc gtacaagcag catctccggc
     4741 ctcacagttt cacgcacgac acagaatcgc ccgccacgac atccagcgcg cgtaacactt
     4801 ccggcggcaa cgatagttcg tctgaacgct ccagcggcgc aagatcggtc acgaccgcac
     4861 gaaaacgctc ttccctcgta ttactgacca gataaggcaa tggggagaca tcacgtaccg
     4921 ccaccgacgc gccggcaagc tgatgcgtcg tcgatgcgga gaaagaacgc agcgagttcc
     4981 tgtgcgcctg caaaatcggc ccaccgtcga agatatcgat gaattcatcc ggctcgaaac
     5041 cttcttgcga caggatacgg aacggcagct cgcctgcgcc atgcgcctgt cccatggcgg
     5101 tctgcgcctc gccgggcaac agcggtacgt agaccggata atgcggcatc at
//
LOCUS       DASRDH010000205         3822 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1167477,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000205 DASRDH010000000
VERSION     DASRDH010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 3822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3822
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1167477"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="VFA14_04670"
     CDS             complement(<1..373)
                     /locus_tag="VFA14_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin"
                     /protein_id="HZS80703.1"
                     /translation="MAAHSMSIRFSVSSSCFVEQYQEQKPLLMKGAASTDDFSWRDAN
                     EIFERSDITSEDFKLSYDGIRLKNEYVESYLDVGALRHRLIKPAVYDFLRKGATLIAN
                     KISNEPKVHAFSKRVADYTGRR"
     gene            complement(414..1073)
                     /locus_tag="VFA14_04675"
     CDS             complement(414..1073)
                     /locus_tag="VFA14_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS80704.1"
                     /translation="MMGQIFPWLWAALMTLALSLMYWASMISYMAAGLMLLFFLKKRA
                     ASWLNPAGVSTKNGVHWRAGYMTRLISNSCSLAICSSRDKCIVQEVQHFKAFFRIFLY
                     THKKTVLFSSNKLSGNFLGGTLSQKPEGIHPMRELNINDELELVTGATTAGNIALVLA
                     SVGASAAATAIAGAAFGGPAGGVAGAIVGAGVGIGYALSGGTFERIDQAGTDYCGCNY
                     H"
     gene            complement(1090..2505)
                     /gene="glnA"
                     /locus_tag="VFA14_04680"
     CDS             complement(1090..2505)
                     /gene="glnA"
                     /locus_tag="VFA14_04680"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HZS80705.1"
                     /translation="MARTAAEVLKLVKDNEVKFVDFRFADTRGKEQHVTVPVSHFDID
                     KFESGHAFDGSSIAGWKGIEASDMLLIPDPNTANIDPFMEETTLFMQCDVIEPSDGKG
                     YDRDPRSIAKRAEAYLKSSGLGDTAYFGPEPEFFIFDSIRWDEDMSGCFVKINSEEAS
                     WSSGAKLEGGNSGHRPTVKGGYFPVPPVDSFQDMRSEMCLILESLGIPVEVHHHEVAG
                     AGQNEIGTRFSTLVERADWTQNLKYVVWNVAHTYGKTATFMPKPLVGDNGSGMHVHQS
                     IWKDGKNLFAGDGYAGLSETALFYIGGIIKHAKALNAITNPGTNSYKRLVPGFEAPVK
                     LAYSARNRSASIRIPHVANPKGRRIETRFPDPLANPYLCFAALLMAGLDGIQNKIHPG
                     EAASKDLYHLPPEENRLIPTVCASLEEALEHLNKDREFLTRGSVFTDSMIDAYLELKE
                     QEVQRYRMTTHPIEFDMYYSL"
     gene            2732..3196
                     /locus_tag="VFA14_04685"
     CDS             2732..3196
                     /locus_tag="VFA14_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007880081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HZS80706.1"
                     /translation="MTSTLEILSSAQRRAQQNQLPYAGAVTPQEAFALLQDNATVKLV
                     DVRTKAERDWVGQVSLAAEQHLAVQWNLYPEGVPNPHFLEQLSAVANKDMILLFLCRA
                     DIRSRHAAKLAADNGYLQCYDVLEGFEGSKDSMGHRKTIEGWCKAGLPWVGA"
     gene            complement(3345..>3822)
                     /locus_tag="VFA14_04690"
     CDS             complement(3345..>3822)
                     /locus_tag="VFA14_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011615071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HZS80707.1"
                     /translation="LVHPPYRVDGTAVFLTGNINTVPVALLHNLKHNHILHKRVLLLK
                     ISIWDVPFVADAKRLMLKDMGGDIYLLRAAFGFKEAPKVNKVLELVTAQFDMKFDLMD
                     TSFFLARETVVPSKLLGMSLWREHLFAWMYQNGAKSSDFFQIPANRIVELGTKVEI"
BASE COUNT          905 a          982 c         1036 g          899 t
ORIGIN      
        1 cccgtcttcc cgtgtaatcc gcgacacgtt tggagaatgc gtgaactttg ggctcgttgc
       61 tgattttgtt tgcgatcagt gtcgcgcctt tgcgcaggaa atcgtaaacg gctggtttga
      121 ttagtctatg ccgcaaggct ccaacatcca gataactttc gacatactca tttttgagcc
      181 ggatgccgtc gtaagacagc ttgaaatcct cggaggtaat atcgcttctc tcaaagatct
      241 cgttggcgtc acgccaggaa aaatcgtcgg tcgaggcagc acctttcatt aggagcggct
      301 tctgttcctg gtactgctca acgaaacaag aggatgaaac actgaatcga atactcatgg
      361 aatgggcggc caagcttgac gtcgcaaggg caagccaagt gaaagtcagg tgattagtgg
      421 tagttgcaac cgcagtagtc agtgccggcc tggtcaatgc gttcaaatgt gccgccactt
      481 agggcataac caatgccaac acctgctccg acgatagctc ctgccacacc gccagccggg
      541 ccaccgaaag cagcaccagc aattgctgtc gcagctgctg aggccccaac gctagccaag
      601 actagggcaa tattgccagc ggtggtggcc ccggtaacaa gctccaattc atcattaatg
      661 ttcagttctc tcattggatg gattccttct ggtttttgag ataaagtacc gcccaagaaa
      721 ttccctgaga gcttatttga tgagaataaa actgtttttt tgtgtgtata taagaaaatt
      781 ctgaaaaaag ccttgaagtg ttgcacctcc tggacgatgc atttatccct gcttgagcat
      841 attgcgagtg agcaggagtt agagattaac ctcgtcatgt aaccggcgcg ccagtgtacg
      901 ccgttcttcg tcgatacgcc ggctggattc agccaggacg ctgcacgttt cttaaggaaa
      961 aacaggagca tcaagccggc tgccatgtac gaaatcatgc tggcccaata catcagggat
     1021 agcgccaggg tcatcagcgc cgcccacagc cacggaaata tctggcccat caagggcggg
     1081 agtcgatact catagtgagt aatacatgtc gaattcgatg gggtgcgtag tcatgcgata
     1141 acgctgcact tcctgctcct tcagttccag gtaagcatcg atcatgctgt cggtgaagac
     1201 gctgccacgg gtcagaaact cgcgatcctt gttcagatgc tccagcgcct cttccagcga
     1261 agcgcagact gtcggaatca aacgattttc ttccggcggc aggtgataca aatctttcga
     1321 tgccgcttcg cccggatgga tcttgttctg gataccatcc aggcctgcca tcagcagggc
     1381 agcaaagcac aggtagggat tggccagcgg gtcaggaaac cgcgtttcga tacgacggcc
     1441 cttgggattg gcgacatgcg gaatacggat cgaagcagag cgattacgtg ctgagtaggc
     1501 cagcttgacc ggtgcctcaa agcctgggac cagacgcttg tatgagttgg tgccagggtt
     1561 agtaattgcg ttgagcgctt tggcatgctt gatgataccg ccaatataaa acagcgcggt
     1621 ttcggacagg ccggcataac catcgccagc gaacaggttc ttgccatctt tccagatcga
     1681 ttggtggacg tgcatgccag agccattgtc gccaacgagt ggcttaggca tgaaagtggc
     1741 tgtcttgcca taggtatgcg ccacattcca gaccacgtat ttcaggtttt gtgtccagtc
     1801 tgcgcgctcg accagcgtcg agaaacgagt gccgatttcg ttctggccgg cgccggcgac
     1861 ttcgtgatga tgcacttcga caggaatgcc cagggattcg aggatcaggc acatttcaga
     1921 acgcatgtcc tggaaactgt cgaccggagg gaccgggaag taaccaccct tgacagttgg
     1981 acgatggccg ctgttgccac cttcgagttt ggcgccactg ctccatgagg cttcttcaga
     2041 gttgatcttg acgaaacagc cggacatgtc ttcatcccag cgtatgctgt cgaaaatgaa
     2101 gaattccggc tcaggaccga agtaggcagt gtcgcccagt cccgaggact tcaggtaagc
     2161 ttccgcgcgc ttggcgatgg agcgcgggtc gcggtcatag cctttgccat cggatggctc
     2221 gatcacatca cattgcatga acagtgtggt ttcttccatg aacgggtcga tgttggcggt
     2281 gttgggatca gggatcagca acatgtccga cgcttcgatg cccttccagc cggcgatcga
     2341 agaaccgtcg aatgcgtggc cggattcgaa cttgtcgatg tcgaaatggg aaacagggac
     2401 tgtgacgtgc tgctctttac cacgggtgtc ggcaaaacga aaatcaacaa acttgacttc
     2461 attatctttc actaacttca aaacttctgc ggccgtcctt gccatgcgaa tctcctaaaa
     2521 cgggaaaatg taaatttgaa caattggggg gaatgggccg actatgtgtg acttgatgtg
     2581 catatcgata caacagataa tgggtccgtt agtgctttgg atgatagcag attccatgtc
     2641 atcgactgcc ttgtgttggt gctaaatgaa cgttcgatag atcgttttga agtacgcttt
     2701 tgtcatttca attatttttt gaggcaacat gatgacttcc actctcgaaa ttctctcttc
     2761 cgcacaacgg cgtgctcagc aaaaccagct tccctatgct ggcgcagtca cgccgcagga
     2821 agcttttgca ttattgcaag acaatgctac cgttaaactt gtagacgttc gcaccaaagc
     2881 ggaacgtgac tgggtcggcc aggtcagcct ggcggcggag cagcacctgg cggtgcaatg
     2941 gaatctctac cccgaaggcg tgccgaatcc gcacttcctc gaacagctca gcgctgtagc
     3001 gaacaaggat atgatattgc tgttcctgtg ccgcgcggac atacgttcgc gtcatgcagc
     3061 caagctggcg gccgataacg gttacttgca gtgctatgac gttctcgaag gttttgaagg
     3121 cagtaaggac agtatgggtc accgcaaaac catcgaagga tggtgcaagg ccggattgcc
     3181 ctgggtggga gcctagatcc tgtcgtcatg aaattctgat ccagagtgca agacacctgt
     3241 aaggcggcga gaaacgacgg caaatttttg gcatagcgtg cagcaatgct gcgcgcctaa
     3301 tatgcaaaac tgcgtctcca ccagatgtcg gtgacgataa tgacctagat ttccactttg
     3361 gtgcccaatt ccacgattcg gttagccgga atctggaaaa aatccgaaga cttggcgcca
     3421 ttctgataca tccaggcgaa caggtgttcg cgccacagcg acatacccag cagcttgctc
     3481 ggtaccacgg tttcgcgcgc caggaagaac gacgtatcca tcagatcgaa tttcatgtcg
     3541 aactgtgccg tcaccaattc caataccttg ttcactttgg gcgcttcttt gaaaccgaac
     3601 gccgcgcgca gcaggtagat gtcgccgccc atgtctttca gcatcaggcg cttcgcgtcg
     3661 gcgacgaaag gcacgtccca gatactgatc ttgaggagca gcacacgctt gtgcaggata
     3721 tggttgtgct tgagattgtg cagtagcgcc accggcaccg tattgatatt gccggtcagg
     3781 aacactgctg tgccatcgac gcgataaggc ggatgcacca gc
//
LOCUS       DASRDH010000206         3243 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1661712,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000206 DASRDH010000000
VERSION     DASRDH010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 3243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3243
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1661712"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..747)
                     /locus_tag="VFA14_04695"
     CDS             complement(<1..747)
                     /locus_tag="VFA14_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008116779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HZS80708.1"
                     /translation="MTVIKQDDLIESVAAALQYISYYHPVDYIRHLARAYEQEQSPAA
                     KDAIAQILTNSRMCAEGRRPICQDTGIVNVFLKIGMGVRFEGFIGSIADAVNQGVRQA
                     YLHPDNMLRPSIVADPQFERRNTRDNTPAVIHVELVPGDTVDVRIAAKGGGSENKSRF
                     VMLNPSDSLVDWVLKTIPGMGAGWCPPGMLGIGIGGTAEKAMLMAKEVLMDDIDMGEL
                     KQRGPQNKTEELRIELCDRINALGIGAQGLG"
     gene            complement(805..1416)
                     /locus_tag="VFA14_04700"
     CDS             complement(805..1416)
                     /locus_tag="VFA14_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017454392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqhA family protein"
                     /protein_id="HZS80709.1"
                     /translation="MNDIHPTPSDKKIGILPNLIFMSRWLQLPLYLGLILAQCVYVYH
                     FWVELSDLIGAALGNPSSLAHLLDAVAIEGAKRPDKLNEQTIMLVILALIDVVMISNL
                     LIMVIVGGYETFVSRMNLEHHPDQPEWLSHVNASMLKVKLGTAIIGISSIHLLKTFIN
                     ANLYDVKTLVAQTGIHITFLLSAIAIAYCDRLMAHPQQDQEEH"
     gene            1604..>3243
                     /gene="acs"
                     /locus_tag="VFA14_04705"
     CDS             1604..>3243
                     /gene="acs"
                     /locus_tag="VFA14_04705"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017454393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HZS80710.1"
                     /translation="MANIETFKQENRVFASPAAFVRQATVSGMEAYDALCAEAEHDYE
                     GFWARLARDNLQWKKPFTRTLNEADAPFFKWFEDGQLNVSHNCLDVHLDNGNADKTAV
                     IFESDDAQVTRLTYRELHTKVCRCANGLKSLGVRKSDRVVIYMPMSVEAIAAIQACAR
                     IGATHSVVFGGFSARSLQERMIDAGAVAVITADCQMRGGKQLPLKSVVDEALAMGGCD
                     TIKTVIVYRRAGADINWNAGRDLWLHDLIASQSDVCEPEWVDAEHPLFILYTSGSTGK
                     PKGVQHACGGYLLWAALTMKWTFDIKPDDIYWCTADIGWITGHTYIAYGPLATGATQI
                     VFEGIPTYPDAGRFWDMVQRHKASIFYTAPTAIRALIKAADADATIHPSQYDLSSLRL
                     LGSVGEPINPATWMWYYQHVGAEKCPIVDTFWQTETGGHMIAPLPGATPQVPGSCTLP
                     LPGISAAIVDETGQDLPNGNGGALVIKRPWPSMIRTIWNDPERFRKSYFPAEFGGKTY
                     LAGDGAVCNGDNGYFTITGRIDDVLNVSGHRMGTMQIES"
BASE COUNT          712 a          992 c          908 g          631 t
ORIGIN      
        1 gccgagacct tgcgcgccga tgcccagcgc attgatccgg tcacacagct ctatgcgcag
       61 ttcttcggtt ttgttttgcg gaccgcgctg cttcagctcg cccatatcga tgtcgtccat
      121 caacacctcc ttggccatca gcatggcttt ctcggcggtg ccgccgatgc cgatgccaag
      181 catgcccggc ggacaccagc ccgcgcccat gcccggtatg gtcttcaaga cccagtccac
      241 cagcgagtcg gaaggattga gcatgacgaa ccggctcttg ttttcggagc cgccgccttt
      301 ggcggcgatg cgcacatcga cggtgtcgcc cggcaccagt tccacgtgga tcacggccgg
      361 ggtgttgtcc cgggtattcc tgcgctcgaa ttgcgggtcg gccacaatgg agggacgcag
      421 catgttgtcc gggtgcaggt aagcctggcg cacaccctgg ttgacggcgt cggcgatgga
      481 accgataaaa ccttcgaagc gcacgcccat gccaatcttc aggaatacgt tgacgatgcc
      541 ggtatcctgg cagatcggac gcctgccttc ggcgcacatg cgcgaattgg tcaggatctg
      601 ggcaatggca tccttggctg ccggactctg ctcctgctca taagcgcgcg ccagatgccg
      661 gatataatcc accgggtggt agtaactgat gtattgcagc gcggcggcaa cggattcaat
      721 cagatcgtct tgcttgatga cggtcattac tggttctctc gggatgaatg gaaaagttgc
      781 ggtctgactg tttcagcagg ctttctagtg ctcctcttga tcctgctgcg gatgcgccat
      841 cagccggtcg caataggcaa tcgctatggc ggacagcaga aaggtaatgt gaataccagt
      901 ctgcgccacc aaagtcttga catcataaag attggcgttg ataaaggtct tgagcaggtg
      961 gatcgatgag atgccgatga tcgccgtacc cagcttgacc ttgagcatcg aggcattcac
     1021 gtgcgacagc cactcgggct ggtcgggatg gtgttcgagg ttcatgcgcg agacgaaagt
     1081 ttcatagccg ccgacgatca ccatgatcag cagattggaa atcatcacca cgtcgatcaa
     1141 cgccagtatc accagcatga tggtctgctc gttgagcttg tcgggtcgct tcgcgccttc
     1201 gatggcgacg gcgtcgagaa ggtgtgccag cgaggaggga ttgcccagcg ccgcaccgat
     1261 cagatcgctg agttcgaccc agaaatgata aacgtagacg cactgcgcca gaatcagccc
     1321 cagatacaaa ggcagctgca accagcggct catgaaaatc agattgggta ggatgccgat
     1381 ttttttatcg gaaggtgtag gatgaatgtc gttcattgca ttggggcaaa tcgtaaaggt
     1441 atgaaaccgg atggattaga acgtactccc gacaaacggg tgaattcagg cccttatttt
     1501 acacttcctt gccctgttct taccgattca tcagcggcag ataggataat gcctatagat
     1561 gagcgaccgt agatcgtcat cgtgaaaaaa cagggaggct accgtggcaa atatcgaaac
     1621 cttcaaacag gaaaaccggg tattcgcctc accggcagcg ttcgtcaggc aggctaccgt
     1681 gtcgggcatg gaagcttacg acgccttgtg cgccgaagcc gagcacgact atgaaggctt
     1741 ctgggccaga ctggcccgcg acaacctgca atggaaaaaa ccattcacca ggacgctgaa
     1801 cgaagcagat gcgccattct tcaaatggtt cgaagacggt cagctcaatg tttcgcacaa
     1861 ctgcctcgac gtccatctcg acaatggcaa cgccgacaag accgccgtca tcttcgagtc
     1921 cgacgacgcc caggtcacca ggctcaccta tcgcgaactg cacacaaaag tctgtcgctg
     1981 cgccaacggc ctgaagtcgc tcggcgtcag gaaaagcgac cgcgtcgtca tctatatgcc
     2041 gatgtcagtc gaagccattg ccgccataca agcctgcgcc cgcattggcg ccacgcactc
     2101 ggtcgtgttc ggcggctttt ccgccaggtc gctacaggaa cgcatgatcg atgccggcgc
     2161 cgtcgcagtc atcaccgccg actgccagat gcgcggcggc aagcaactgc ccctgaaatc
     2221 cgtcgtcgac gaagcgctcg ccatgggcgg ctgcgacacc atcaaaaccg tcatcgtcta
     2281 caggcgcgct ggagccgaca tcaactggaa cgcaggccgt gacctctggc tgcacgatct
     2341 catcgccagc cagtccgatg tctgcgaacc ggaatgggtc gatgccgaac atccgctatt
     2401 catcctgtac acctccggct ccaccggcaa accgaagggc gtgcaacacg cctgcggcgg
     2461 ctatctgctg tgggccgcgc tgacgatgaa atggaccttc gacatcaaac ccgacgacat
     2521 ctactggtgt accgccgaca tcggctggat caccggccac acctacatcg cttacggccc
     2581 gctggcaacc ggcgccacac agatcgtgtt cgaagggata ccgacctacc ccgacgccgg
     2641 ccgcttctgg gacatggtgc agcgtcacaa agccagcatt ttttacaccg cgccgacggc
     2701 gatccgcgcg ctgatcaaag ccgccgacgc tgatgcaaca atccatccca gccaatacga
     2761 cctgtcgtca ctgcgtctcc tgggttcggt cggcgaaccg atcaatccgg caacctggat
     2821 gtggtactac caacatgtcg gtgcagagaa atgcccgatc gtcgatactt tctggcaaac
     2881 cgagaccggc ggacacatga tcgcgccgct gccgggcgcg acaccgcagg tgccaggctc
     2941 ctgcacgctg ccgttgccgg gcatcagcgc cgccatcgtc gatgaaaccg gccaggatct
     3001 gcccaacggc aacggcggcg ccctcgtcat caagcgccca tggccatcga tgatccgcac
     3061 catctggaac gatcctgaac gtttcaggaa aagctacttc cccgcagaat ttggcggcaa
     3121 aacctacctc gccggcgacg gcgcggtatg caacggggac aatggctatt tcaccatcac
     3181 cggccgcatc gacgacgtac tcaacgtctc cggccatcgc atgggcacca tgcagatcga
     3241 gtc
//
LOCUS       DASRDH010000207         4765 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_683794,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000207 DASRDH010000000
VERSION     DASRDH010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 4765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4765
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_683794"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="VFA14_04710"
     CDS             <1..417
                     /locus_tag="VFA14_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HZS80711.1"
                     /translation="IVLEHALTWPGGGITLHGRLDRIDEHATGERAVLDYKTRALPAL
                     AAKLKDVEDHQLPFYGLLSGQTMAAAHYVALEVFRDKTGDVAAPDFAWRQQALQEQIV
                     TVMQAVMQGAPLPANGIASACQYCEVRGLCRKGDWQ"
     gene            393..3767
                     /locus_tag="VFA14_04715"
     CDS             393..3767
                     /locus_tag="VFA14_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HZS80712.1"
                     /translation="MPQGGLAMSAMQNDVAPVSAYEINGQPATAAAFIAAACDPRHSV
                     VVEACAGSGKTWLLVARMLRLLLAGARPAELLAITFTRKAAQEMRERLMALLHELALA
                     PAEKACALLLERGVAAEELPRVMPQARVLYEQILAGQQGLSIDTFHSWFARLLRIAPL
                     VSGVPHGYALTEKNGELINEAYRRFMQSVQQPSRQEVKQALLALYDLAGDSGARRLLD
                     AFLDRRAEWWAASMSPLSSGEMPLRWLENLCGPDARCDARLEVWSDGALQARLSEIAA
                     LLGKGAAANQKRALIIETLLSGEASPENFAALATQFYDDADKPRGNNARAKALKAALV
                     AHYGVDEEAAVALFEEVFASTGNALRRLQRRSAEGTVLKLNRALFIAGRAYLDAYQEV
                     KTGQRVFDFADLEWQAYRLLSDEESAAYLQSRLDARYRHILLDEFQDTNPLQWSIVRA
                     WLRAYGEDAAQPGVFVVGDPKQSIYRFRRAEPRVFAAARDLLARQGAAVLRANQTRRN
                     ATVIVDVLNSSFAGRNALFAPQTTSGMQGGGVWKLPLVQAQAVSAIGMPMRNPLTTPR
                     DEEEDARRRDEGALVAQALLQARHQVSVRAEDGERKLQWNDVMLLVKKRRHLRAYESA
                     LREAGIPFVSDKRGGLLDALEVADLIALLTFLNTPSDTRALAHVLKSPIIGASDDDLI
                     ALAQRDEGDWWHRVKAQCDSDKASPAITRAAHLLTQWIAAAPHHPVHDLLDRILHQGR
                     LAVRYAQSAAPAMRQQTLGNIDAFVALALDLDAGRYSGLPRFIAALRILLQHAESDAP
                     DEADIDAAADAVRILTVHSAKGLEAPVVVMLDTNHSDPADDHVGMLCDWPQDEDAPTH
                     FSAYGRKSERGLARDRWFDAEEGFRQQEDWNLLYVAMTRARQLLIVSGVAGARGAVAD
                     GMQEGSWYHRLFDRAGGVAGMEEMTPQAQREEVAASVGERFSLALFDPAPQPAPLSAQ
                     EHDSSDAIAEGLALHALLERMTHVCAWPPVLPDAATIARWLPCPHVLAATIRAQAEKI
                     LSQPALERFFNPALYRHARNEMDIMTGDGVLRCDRVVVFDDEVWILDYKRRLLDSERA
                     AYAAQLARYRDAAASVFSSRRIRSALIIADGRFWEQ"
     gene            3955..>4765
                     /locus_tag="VFA14_04720"
     CDS             3955..>4765
                     /locus_tag="VFA14_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008325789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HZS80713.1"
                     /translation="MQWTLPSFAFAQQFQWSALLLFGSLLLFGLIAGHVVSKSPWVPR
                     ITGYLIVGFILGGGGLNWLSGDMLHVTNIFADIAVALVLYQLGRYVDIGWLKREKWLL
                     ATVVTSAVLCFVFVSMALLWFGTSQVSALLGGVWAIATAPAVVLAVLRDLKAEGQVTR
                     RLAAMTALNNFVAILAAYALLPVIANESGTSVLMLVEHTLYSLVGSFVLAYLTYWLMM
                     PLARLLGHQGSRQFVLVIAVIALAIGAAHALHLPVMLSMLIFAILSKNLDRQ"
BASE COUNT          829 a         1318 c         1580 g         1038 t
ORIGIN      
        1 atcgttctgg aacacgcgct gacatggccg ggcggtggta ttacattgca tggccgtctc
       61 gaccgcatcg atgaacatgc cacaggcgag cgtgccgtac tcgactacaa gactcgcgcg
      121 ctgccggcgc tggccgctaa actgaaagat gtcgaagacc atcagttgcc gttctacggt
      181 ttgctgtccg ggcaaaccat ggcggcggcg cattacgtcg cgctggaagt tttcagggat
      241 aagactggcg atgtcgccgc gccggatttc gcatggcggc aacaggcgtt gcaggaacag
      301 atcgtcaccg tcatgcaggc cgtgatgcaa ggcgcgccgt tgccggccaa tggcatcgca
      361 tcggcttgtc agtattgtga agtacgcggt ctatgccgca agggggactg gcaatgagcg
      421 ccatgcagaa cgacgtcgcg ccggtttccg cttatgaaat caatggtcag cctgctaccg
      481 ccgctgcctt cattgccgct gcctgcgatc cgcgtcattc cgtggtggtg gaagcctgcg
      541 ccggtagtgg caagacctgg ctcctggtgg cacgcatgct gcgtctgctg ctggccgggg
      601 ccagaccagc cgagttgctg gcgattacct ttacccgtaa agccgcgcag gaaatgcgcg
      661 agcgcttgat ggcgctgttg catgaactgg cgctggcacc ggcggaaaaa gcgtgcgcgc
      721 tcttgctgga gcgcggtgtc gccgcagagg aattgccgcg cgtcatgcct caggcgcgtg
      781 tgctatacga acagatactg gcgggccagc aggggttgtc catcgacacc ttccatagct
      841 ggtttgcccg cctgctgcgc attgcgccac tggtttccgg cgtgccgcat ggttatgcgt
      901 tgaccgaaaa aaatggcgaa ctgatcaacg aagcctatcg ccgtttcatg caatccgtgc
      961 agcagccatc gcggcaggaa gtgaagcagg ccttgctggc gctgtacgat ctggccggcg
     1021 acagcggcgc caggcgtttg ctggatgcct tccttgatag gcgcgctgag tggtgggcgg
     1081 ccagcatgtc acccttgtct tccggggaga tgccgctgcg ctggttggaa aatttgtgcg
     1141 gaccggatgc gcgctgcgat gcgcgtctgg aggtctggtc ggacggggcg ttgcaggcgc
     1201 gtttgtctga gattgccgcg ctgctgggca aaggcgccgc cgccaaccag aagcgcgcgc
     1261 tcatcatcga gacgctgctt tcgggagagg catcgccgga gaatttcgcc gcgctcgcga
     1321 cgcagtttta cgatgacgcc gacaaaccac gtggcaacaa tgcccgcgcc aaggctttga
     1381 aagcggcgct ggtcgcgcat tacggcgtgg atgaagaggc tgccgttgcg ctgttcgaag
     1441 aagttttcgc gtcgaccggc aatgccttgc gccgcttgca gcggcgcagc gccgaaggca
     1501 ccgtgctgaa gctcaatcgc gccttgttca tcgccggccg agcttatctc gatgcctatc
     1561 aggaggtcaa gaccgggcaa cgcgtcttcg acttcgccga tctcgaatgg caggcgtatc
     1621 gtctgttgag cgatgaagag agtgcggcgt atctgcaaag ccgcctcgat gcgcgttaca
     1681 gacacattct gctggacgaa tttcaggata ccaatccgct gcaatggagc atcgtgcgcg
     1741 cctggttgcg tgcctatggc gaggatgcgg cgcagccggg cgtgttcgtg gtgggtgatc
     1801 cgaagcaatc gatttatcgc ttccgccgtg ccgaaccgcg cgtgttcgcg gcggctcgcg
     1861 acttgctggc gcggcagggt gccgccgtcc tgcgcgccaa ccagacgcgc cgtaatgcga
     1921 ctgtcatcgt cgatgtactc aatagcagtt ttgccggccg taatgccttg ttcgcgccgc
     1981 agacgacctc gggcatgcag ggcggcggtg tgtggaagct gccgctggta caggcacagg
     2041 cggtatctgc aatcgggatg ccaatgcgca atccgttgac cacgccgcgt gatgaggaag
     2101 aagatgcgcg ccgccgtgat gagggcgcat tggtcgcgca agcgctcttg caggcgcgtc
     2161 atcaggttag cgtcagggcc gaagacggcg aacgcaagct ccagtggaat gatgtgatgc
     2221 tgctggtgaa gaagcgccgt catctgcgtg cttatgagag cgctttgcgc gaggccggca
     2281 ttccgttcgt ctccgataag cgcggtggct tgctcgatgc gctggaagtc gccgacctga
     2341 tcgcgctgtt gacttttctg aacacgccca gtgacacgcg cgcgctggcg catgtgctga
     2401 agagtccgat catcggggcc agcgacgatg atctgattgc gctggcgcag cgcgatgagg
     2461 gcgactggtg gcaccgtgtc aaggcgcagt gcgatagcga caaagcatca ccggcaatca
     2521 cgcgcgctgc tcatttgctg acgcagtgga tagcggcggc gccgcatcat ccggtacacg
     2581 atctgctcga caggatattg catcagggcc ggttggcggt gcgttacgcg caatcggcag
     2641 cgcccgcgat gcgccagcaa accttgggca atatcgacgc tttcgtcgcg ttggcgctcg
     2701 atctcgatgc cgggcgttat tcaggtttac cgaggttcat cgctgccttg cgtatcttgc
     2761 tacagcacgc cgaaagcgat gcgccggatg aggccgacat cgatgccgcc gccgacgcgg
     2821 tgcgtatttt gactgtgcat agcgccaaag ggctggaagc gccggtggtc gtgatgctcg
     2881 acaccaacca tagcgatccc gccgatgacc atgtcgggat gttgtgtgac tggccgcagg
     2941 acgaagatgc accgacgcat ttttccgcgt atggccgcaa gagtgagcgt ggacttgcgc
     3001 gtgatcgatg gttcgacgcg gaagagggtt tcagacaaca ggaagactgg aatctgcttt
     3061 acgtcgccat gacgcgcgcc aggcaattgt tgatcgtcag tggcgtggca ggcgcgcgcg
     3121 gcgcagtggc ggatgggatg caggagggga gctggtatca ccgcctgttc gatcgcgccg
     3181 gtggggttgc cggtatggaa gaaatgacgc cgcaggcgca gcgtgaggag gtagcggcaa
     3241 gcgtcgggga aaggttttca ctggcgttgt tcgatcccgc gccgcagcca gcgccgctgt
     3301 ctgctcagga gcatgacagc agtgatgcca tcgcagaagg tctggccctg catgccttgc
     3361 tcgagcgcat gacccatgtg tgcgcgtggc cgccggtgct gcctgatgca gctaccatcg
     3421 cacgctggct gccgtgtccg catgtgctgg cggcgacgat acgcgcgcag gctgaaaaga
     3481 tattgtcgca acccgcgctg gagcggtttt tcaatccggc cctgtatcgc catgcgcgca
     3541 acgagatgga catcatgaca ggagatggcg tgctacgctg cgatcgggtc gtggtgttcg
     3601 acgatgaagt ctggatactt gattacaagc gcaggctgct cgacagcgag cgcgccgcgt
     3661 atgccgcgca gctggcgcgt tatcgcgatg cggcagcgtc cgtgttttcc agccggcgga
     3721 tcaggtcagc gctgattatt gcggatggcc ggttctggga acaatgatga gacttcagaa
     3781 cctattcacg atctcattgt gaacatgacg attacgatgt aggaaaatat tccgtttatt
     3841 tccaaactgg tttatcctct gcccggctgt cagcatcact ttacattcat catcagcgta
     3901 tcgtctgaca ggcgcgagct tcgtcgccgg atgcgctcga ttttaggggg agctatgcag
     3961 tggactcttc cgtctttcgc ctttgcgcaa cagtttcagt ggagcgcgtt gctgctgttt
     4021 ggtagcttgt tgctgttcgg gttgattgcc ggtcatgtcg tgtcgaagtc gccatgggtg
     4081 ccgcgcatca ctggttatct gatcgtcggt ttcattctcg gcggcggtgg cttgaactgg
     4141 ttgtccggcg atatgctgca tgtcacgaat atctttgccg atatcgccgt cgcgctggtg
     4201 ctataccagc ttggacgtta tgtcgacatc ggttggctca agcgcgagaa gtggctactg
     4261 gcgaccgtgg tgacatcggc ggtgctgtgt ttcgttttcg ttagcatggc tttgttgtgg
     4321 ttcggcacgt cgcaggtatc ggcgctgctc ggtggtgtct gggcgattgc cacggcgccg
     4381 gcggtggtgc tggcggtatt gcgcgacctg aaggcggaag gccaggtcac gcggcgtctg
     4441 gcggccatga cggcgctcaa taatttcgtc gctatcctgg ctgcttacgc cttgctgccg
     4501 gtgatcgcga atgagtccgg tacgtcggtg ctgatgctgg tcgagcacac gctgtattcg
     4561 ctggtcggct ccttcgtgct ggcttatctg acatattggc tgatgatgcc gctagcgcgt
     4621 ttactggggc accagggtag ccgccagttc gtgctggtga tcgccgtcat cgcattggcc
     4681 atcggcgcgg cgcatgcgct gcatttgccg gtgatgctga gtatgttgat ttttgccatt
     4741 ctgtcgaaga atctggatcg tcaat
//
LOCUS       DASRDH010000208         3903 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1021989,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000208 DASRDH010000000
VERSION     DASRDH010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 3903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3903
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1021989"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="VFA14_04725"
     CDS             complement(<1..305)
                     /locus_tag="VFA14_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007877259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HZS80714.1"
                     /translation="MKKNRLVCLGVAISLLAACSTTLYTTAPVVKRTTDSSKPAVNAT
                     RPATAAAARPERKGFYRVRKGDTLYRIALESGQSYRDIVAWNKLTNPDDIKVDQVLRV
                     "
     gene            complement(463..1329)
                     /locus_tag="VFA14_04730"
     CDS             complement(463..1329)
                     /locus_tag="VFA14_04730"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HZS80715.1"
                     /translation="MTGKPSRFPLSLSSVMEKKNKFPPAASSRSAVPFATPARTVVTP
                     QTATRNAARDAGDQTTTRSAWPTPHDSQQASLVSDAVRKAMVVRTAKQGVSDTKVLAA
                     METIPRHMFVEPALASQAYIDASLPIGYHQTISQPYIVARMIEIMLDNKKDKVLDRVL
                     EIGTGCGYQAAVLSLVAKEVYSIERIKPLHELAKTNLRPLRIANIRLHYGDGMLGLPQ
                     VAPFDGIILAAAGLKVPQALLEQMAIGGRLVAPIGERQQMLTSIERTGKFEWTSETLE
                     ECHFVLLCPGTL"
     gene            complement(1326..2063)
                     /gene="surE"
                     /locus_tag="VFA14_04735"
     CDS             complement(1326..2063)
                     /gene="surE"
                     /locus_tag="VFA14_04735"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HZS80716.1"
                     /translation="MNILISNDDGYLAPGIIALADALSAIADITVVAPDSNRSCSSNS
                     LTLDRPLWVERAANGFYYINGTPSDCVHVALTGLLPVLPDLIVAGINQGQNMGDDTLY
                     SGTVAAATEGFLLGIPAIAFSQLHKGWEHLDSAARMAREIVERQFTTLFKPYLLNINI
                     PNLPYAALKSCVATRLGKRHAAEPVHKMRDPHGKDMYWIGPPGAVKDAGEGTDFHATA
                     QGHVSITPLQIDLTHAAQLANLRKALA"
     gene            complement(2136..>3903)
                     /locus_tag="VFA14_04740"
     CDS             complement(2136..>3903)
                     /locus_tag="VFA14_04740"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007877253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HZS80717.1"
                     /translation="ISTGTKNLQDQLYLRDIPMVRKALNAPVLVALLKGRSNYVCHFH
                     LQRTLQNGSMTSRDDIGHLRKISRFMKTTSSGDKAELSMVPETAPIWNLVTSTRDTCF
                     GAECQYYQDCFVMKARKEAQQADVVVVNHHLFFADVALKDTGVAELLPAANTVIFDEA
                     HQLPETATMFFGDSVSTSQILELCRDVLAEGLSHARDGADWTGVITPVDRAARDLRLA
                     IPEDIMRLALQQIAPSSMFFPALASLRDSMDNMIAVLEKQAERAETIEQCRTRAIALS
                     QQLDNWNSAQDKPGGEDDQTDVLWIEAFTSSLRLHRTPLSIAPIFSKQREGVLRAWIF
                     TSATLAVKSDFHHYASQMGLSAEPAQSWPSPFNYEQQGLLYVPNNLPPPNSFGYTDAV
                     IDAALPAIIAAGGRTFLLCTTMRAVNRAAERLREEFAQRDLPFPLMVQGEAGRTELLD
                     RFRSAGNGVLIGSQSFWEGVDVRGDALSLVIIDKLPFSPPDDPVLAARIAELEKKGLS
                     GFVHHQLPSAIINLKQGAGRLIRDENDRGVLMICDPRLISRGYGRRIWQSLPPFKRTR
                     EIGAVEDFFAPRVECAAIPGAS"
BASE COUNT          826 a         1107 c         1111 g          859 t
ORIGIN      
        1 acgcgcagca cctgatctac cttgatatcg tcgggattgg tcagcttgtt ccaggctacg
       61 atatcgcgat aactttgccc tgactccagc gcgatacgat acagcgtatc gccttttctt
      121 accctgtaaa aaccttttcg ctcaggacgc gcagcagcag cagtcgcggg cctggtcgcg
      181 ttgacagcgg gtttggaaga atccgtcgtg cgcttcacta cgggagctgt ggtatacagt
      241 gtagtcgaac acgctgcaag cagactgata gccactccca ggcagaccaa acgattcttt
      301 ttcatgctca ttacattcgt ttctacgaca atgatgtcgg tcctccgcat cgcgtgagaa
      361 gttcatcgtg acaggcaagg cctggacatc cgttctgttg ccaggatcca tgctgcttat
      421 ccgtgcgtta gaataagttc ccgatcttgt cgggagtcgt tcttacaggg taccggggca
      481 caacagcaca aaatggcatt cttccagcgt ttcgctggtc cactcaaatt tgccggtacg
      541 ctcaatcgat gtcagcattt gttgacgctc cccgattggc gctaccagac gcccgccgat
      601 cgccatctgt tcgagcaaag cctgtggaac tttcagtccc gcggcagcga gaatgatacc
      661 gtcgaacgga gcaacctgcg gcagacctag cataccatct ccgtagtgca aacggatatt
      721 ggcaatacgc agcggccgca aattggtttt agccagttca tgcaaaggtt tgatgcgttc
      781 gatcgaatag acttccttcg ccaccagcga taaaacggca gcctgataac cacagccggt
      841 gccgatttcc agcacgcggt caagcacttt atccttcttg ttgtcgagca tgatttctat
      901 catgcgggcc acgatataag gttgcgaaat ggtctggtgg tatccgatcg gcaacgatgc
      961 gtcgatgtaa gcctggctgg ccagagccgg ctccacgaac atatggcgcg gtatcgtttc
     1021 catggcggct agaactttgg tatcgctgac accctgtttg gccgtgcgca ctaccatggc
     1081 tttacgcacg gcgtcagaca ctaaagacgc ctgctggctg tcatgcggcg tcggccaggc
     1141 gctgcgcgtg gtggtctgat cgcccgcatc ccgggcagcg tttctggttg ccgtctgtgg
     1201 tgtcacgact gtgcgcgccg gtgtcgcgaa tggcactgcg gagcggctcg aagcagccgg
     1261 cgggaatttg ttttttttct ccatgacaga agatagcgac agcgggaaac ggctcggttt
     1321 tccggtcatg ccagtgcttt cctcaggttg gcgagctggg cggcatgcgt cagatcgatc
     1381 tgcaacggcg taatcgatac atgtccctgc gcagtcgcat gaaaatcggt accttcgccg
     1441 gcatccttga ctgcaccggg cggaccgatc cagtacatgt ccttgccatg tggatcacgc
     1501 atcttgtgga ccggctccgc ggcatggcgt ttacctagcc gtgtcgccac gcaggatttc
     1561 aacgcggcat acggcagatt aggaatgttg atattgagca gatacggttt gaaaagcgtc
     1621 gtaaactggc gctcgacgat ttcacgcgcc atacgcgcgg cgctgtcgag gtgttcccaa
     1681 cccttatgca attgtgagaa ggcaatcgct ggaatgccga gcagaaaacc ttcggtcgct
     1741 gccgccacgg tgccggaata cagcgtatca tcgcccatat tctggccctg gttgatgccg
     1801 gcgacgatca gatcagggag taccggcagt aatcctgtca aggcgacatg cacgcaatcg
     1861 gacggtgtgc cgttgatata atagaaaccg ttagcggcgc gttccaccca taacggacga
     1921 tccagcgtca gcgagttgga actgcacgat cggttgctat ccggcgcgac taccgtaatg
     1981 tcggcaatgg cggataaggc gtcagccagt gcaatgatgc ctggagcaag atagccatcg
     2041 tcattactaa tgagaatatt catcccgcca ttctacccga agccctgcgc cgtattcgta
     2101 gagaaatagg gctggatacg gtgtttctcc tgtattcagg atgcgccggg gatcgcggca
     2161 cattcaacgc gcggggcgaa gaaatcttcc accgcgccga tttcgcgggt gcgtttgaac
     2221 ggcggcaggc tttgccagat acgtcggcca tatcccctgg agatcaggcg cggatcgcag
     2281 atcatcaata cgccccgatc gttctcatcg cgaatcagac ggccagcgcc ttgcttcagg
     2341 ttgatgatcg ctgagggtag ttgatgatgc acgaaaccgc tcaatccctt cttttccagt
     2401 tcggcgatcc gcgctgctag taccggatcg tccggcggtg aaaaaggtag tttgtcgatg
     2461 atcaccagtg acagcgcatc accgcgcaca tcgacgccct cccagaaact ctggctgccg
     2521 atcagcacgc cgttaccggc tgagcggaag cggtcgagca attcagtacg cccggcttcg
     2581 ccctgcacca tcagcggaaa aggcaagtca cgctgcgcga attcttcacg cagacgctca
     2641 gctgcgcgat tgaccgcgcg catcgtcgtg catagcagga aggtacggcc gccggcggca
     2701 atgatggccg gcagcgccgc atcgatcacg gcatcggtat agccgaacga attcggtggc
     2761 ggcagattgt ttggcacata cagcaagccc tgctgctcat agttgaacgg actcggccag
     2821 gattgcgccg gctcggccga caaacccatt tgtgacgcat agtgatgaaa atcgcttttc
     2881 accgccagcg tcgccgaggt gaaaatccag gcgcgcaaca cgccttcgcg ctgcttgctg
     2941 aaaatcggcg cgatcgatag cggtgtgcga tgcagccgca acgacgaggt gaaggcttcg
     3001 atccacagca catcagtctg atcatcttcg ccgccaggct tgtcctgcgc gctgttccag
     3061 ttgtcgagct gctgcgacaa ggcgatggcg cgggtgcggc attgttcgat agtttcagcg
     3121 cgctcggcct gcttttccag cacggcaatc atgttgtcca tgctgtcgcg caggcttgcc
     3181 agcgctggaa aaaacatgct cgacggcgcg atctgctgga gcgccaggcg catgatgtcc
     3241 tccggaatag ccagtcgtag atcgcgcgcg gcacgatcga ccggcgttat caccccggtc
     3301 cagtcggcgc catcgcgtgc atgtgacaaa ccctcggcca gcacgtcgcg acacaactcc
     3361 agtatttgcg aggtcgatac gctgtcaccg aagaacatgg tcgcggtttc aggtaattga
     3421 tgcgcttcgt cgaaaatcac agtattggca gccggcagca actcggccac gccagtgtct
     3481 ttcagcgcaa catcggcgaa gaacagatgg tgattcacta ccactacatc ggcttgttgc
     3541 gcttccttgc gtgccttcat gacgaaacaa tcctgatagt actgacactc ggcgccgaag
     3601 caggtatcgc gcgttgatgt caccagattc cagatcggcg cagtctccgg aaccatggat
     3661 aattccgctt tgtcaccgga tgaggtagtc ttcatgaagc gtgaaatttt ccgcaagtga
     3721 ccgatatcat cacgcgaagt catgctgccg ttctggagcg tgcgctggag atgaaaatgg
     3781 cagacgtagt tggaacgtcc cttgagcaag gcgaccagca ccggtgcgtt caatgccttg
     3841 cgcaccattg gaatgtcgcg cagatagagc tgatcctgca ggttcttggt gccggtggag
     3901 atg
//
LOCUS       DASRDH010000209         4776 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_885723,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000209 DASRDH010000000
VERSION     DASRDH010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 4776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4776
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_885723"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..753
                     /gene="ilvC"
                     /locus_tag="VFA14_04745"
     CDS             <1..753
                     /gene="ilvC"
                     /locus_tag="VFA14_04745"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006462004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HZS80718.1"
                     /translation="YAENVAPYARQGATVAFAHGFNIHYGQVVPRADLDIIMIAPKAP
                     GHTVRSTYTQGGGVPHLIAVHQDRSGIARDIALSYAAANGGGRAGIIETSFREETETD
                     LFGEQAVLCGGAVELIKAGFETLVEAGYAPEMAYFECLHELKLIVDLIYEGGIANMNY
                     SISNNAEYGEYVTGPRIINEESKKVMQQVLTDIQTGEYAKSFILENKAGAPTLLSRRR
                     LNAEHQIEIVGAKLRAMMPWIKAGKLVDQSKN"
     gene            838..1584
                     /locus_tag="VFA14_04750"
     CDS             838..1584
                     /locus_tag="VFA14_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007881894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HZS80719.1"
                     /translation="MSRSSQLLFAAILATAAMMAHAQSAQVTGSLIVIPASGAIVVPN
                     DQAHVTLSVEEQDPDKAAAASRVNRKMKQGIDIVKQQDPRAQLKTRGYYTYAVYAEES
                     HSGKQPRPIVGWRVGQYLDVTTTNLEGLSRTVAAAQRILGLNDLRFGLSPEATRKLDE
                     NLINATYRNFSERIASIAMAMGLGSAHAVVESIDFEGSGNDVQNAAPRAAMMRAMTDA
                     PAQEALAEPSFEPGDSTVGMHLVGKVRFNK"
     gene            complement(1648..1723)
                     /locus_tag="VFA14_04755"
     tRNA            complement(1648..1723)
                     /locus_tag="VFA14_04755"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1688..1690),aa:Arg,seq:ccg)
     gene            1928..2464
                     /locus_tag="VFA14_04760"
     CDS             1928..2464
                     /locus_tag="VFA14_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HZS80720.1"
                     /translation="MSDDAHEESAIRTPKQLIVAVLAGFLVPVICIVLLVQYVTNAQK
                     TGAGSDSQSPEMIAARIKPVADDGFTFRDASVPRQLLNGEEVFKTTCAVCHTTGVGGA
                     PKVGDFAAWRARISQGYDKLLSHSIIGLNAMPPKGGNPDLDDVEIARTVVYMANQSGA
                     RFKEPEAPAPAEAPPDNK"
     gene            2570..2872
                     /locus_tag="VFA14_04765"
     CDS             2570..2872
                     /locus_tag="VFA14_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HZS80721.1"
                     /translation="MLKHIVMWTLKEHAEGADKATNARRMKELLDGCKGLVPGMLAFE
                     TALAQPGLEATCDVVLYAEFTSRAALDAYQDHPDHVAIKPFIGAIREARQCMDYPI"
     gene            2902..3324
                     /locus_tag="VFA14_04770"
     CDS             2902..3324
                     /locus_tag="VFA14_04770"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008329454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HZS80722.1"
                     /translation="MSHSLSEIFINGNPYLESLGVEVLEYGDGKASMALLLRPEFMNG
                     WRVVQGGISMTLMDVAMGLAMRTAIPDARAAATVDMNASFLQPAGRAGETLVAQGLVY
                     HRSATMCFCEAQLWNGDTLVARAMGTFKALKCVGMPGN"
     gene            3364..3789
                     /locus_tag="VFA14_04775"
     CDS             3364..3789
                     /locus_tag="VFA14_04775"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007877590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZS80723.1"
                     /translation="MDYSIRIAAWAELRADAMLIRNEVFVAEQRVPAELEMDDMDAVC
                     AHAIAYDAAGMPLATGRLLPDGHIGRLAVRSRRRGQGIGGAVLQALMAVARQRGDREV
                     VLNAQTHAAPFYGSHGFAREGEEFMEAGIPHIRMRAWLA"
     gene            3917..>4776
                     /locus_tag="VFA14_04780"
     CDS             3917..>4776
                     /locus_tag="VFA14_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HZS80724.1"
                     /translation="MAKTPFYKSLYFQVLMAIAIGVLLGHFYPHAGEAMKPFGDGFIK
                     LIKMIIAPVIFCTVVLGIAGMEDMKGVGKTGGLALLYFEIVSTLALVVGLVLVNVLQP
                     GVGMNVDPHTIDAGGIAAYTGPGKMSSTTDFIMNVIPVSMVDAFAKGDVLQVLLISVL
                     FGFALHRFGGRGTLVFDCIEKFSHVLFAMVGAIMKAAPIGAFGAMAFTIGKYGVDSLL
                     SLGKLMGTFYLTCLIFVFVVLGAIARLHGFSVWKFIKYIKEELLIVLGTSSSESVLPR
                     MLVKMENLGV"
BASE COUNT         1001 a         1360 c         1454 g          961 t
ORIGIN      
        1 tacgcggaaa atgtcgctcc atacgccagg cagggcgcta ccgttgcctt tgcgcatggc
       61 ttcaacatcc actacgggca ggtcgtgccg cgcgccgatc tggatatcat catgatcgcc
      121 ccgaaggctc ccggccacac cgtgcgctcg acctacaccc agggtggcgg cgtgccgcat
      181 ctgatcgccg tccaccagga caggtccggt attgcccgtg acatcgccct gtcatacgcc
      241 gccgccaatg gcggcggccg cgccggtatc atcgaaacca gctttcgcga agaaactgaa
      301 accgacctgt tcggtgaaca ggccgttctg tgcggcggtg ccgtcgaact gatcaaggcc
      361 ggctttgaaa ccctggtgga ggccggctac gcgccggaaa tggcctactt cgagtgtctg
      421 cacgaattga aactgatcgt cgatctgatc tacgaaggcg gcatcgccaa catgaactac
      481 tccatctcca acaatgcgga atacggcgaa tatgtcaccg gcccacgcat catcaacgaa
      541 gagagcaaga aggtcatgca gcaggtgttg accgacatcc agaccggcga atacgccaag
      601 agcttcatcc tggaaaacaa ggccggcgcg ccgaccctgc tgtcgcgccg ccgcctcaac
      661 gccgagcacc agatcgaaat cgtcggcgca aaactgcgcg cgatgatgcc atggatcaag
      721 gccggcaagc tcgtggacca gtcgaaaaac taggaacgcg ttcccgatgc cgggcgcgca
      781 ggcatgcgcg ccctggaggg aaccgtatgc agcaccttca tcgtttctgg agtatccatg
      841 tcgcgctcga gccaactatt attcgctgcc atccttgcaa ccgccgccat gatggcgcat
      901 gcgcagtccg cccaggtcac cggcagcctg atagtgatcc ccgcctcagg cgcaattgtc
      961 gtgccgaatg atcaggcgca tgtgacgttg agcgtggagg agcaggatcc ggacaaggcc
     1021 gcggcggcgt cgcgcgtcaa caggaaaatg aagcagggta tcgacatcgt caagcagcag
     1081 gatccgcggg cgcagctcaa gacacgcggc tattacacct atgcggtcta tgccgaagag
     1141 tcgcattccg gcaagcagcc gcgtcccatc gtcggctggc gtgtcggcca gtatctcgac
     1201 gtcaccacga ccaatctgga ggggctgtcc aggacggtgg cggcggcgca gcgtattctc
     1261 ggactgaacg atctgcgatt tggcttgagt cctgaggcga ccaggaagct ggatgagaac
     1321 ctgatcaacg ccacctacag gaatttcagc gagcgcattg cctccattgc catggcgatg
     1381 gggcttggca gcgctcatgc cgtggtggaa agcatagatt tcgaaggctc cggcaatgat
     1441 gtgcagaatg cagcgcccag ggcggccatg atgcgcgcga tgacggatgc gccggcgcag
     1501 gaagcattgg ctgaaccaag cttcgagccg ggcgactcca ccgtgggcat gcatctggtc
     1561 ggcaaggtgc gtttcaacaa ataagagcgt gttcagggtt ttctgcgtat aagaaacatc
     1621 gattcgtgtg accaactccg ggaaaactgg cgcggctggc ggggatcgaa cccacgaccc
     1681 ttggcttcgg aggccaatac tctatccact gagctacagc cgcactggag gtgaacgtcg
     1741 aagaggcgtg aaggataccg tttttcccca tgcatgcaaa gcaattattt tccgttcccg
     1801 atgtcaatag tgcctaacac gctctataat ccaaagcggt gcgggatggc acttttctgg
     1861 acagtcatag acagacactg gtttcgatca aatcaacgct ttcgaagaat atggcacgga
     1921 gcgaacgatg agcgatgacg cacatgaaga atccgcgatc aggacgccga aacagctgat
     1981 tgtcgcggta ctggccggtt ttctggttcc cgtcatctgc atcgtgctgc tggtgcaata
     2041 tgtgacgaac gctcaaaaga ctggcgccgg ctcggacagc cagagtcccg aaatgatcgc
     2101 cgcccgtatc aagccggtcg ccgacgacgg cttcaccttt cgcgacgcca gcgtgcccag
     2161 gcaattgctg aacggtgagg aagtctttaa aacgacctgc gccgtctgtc atacgaccgg
     2221 cgtcggcggc gcgccgaagg ttggcgattt cgccgcctgg agagcgcgca tcagccaggg
     2281 ttatgacaaa ctgctgtcgc actcgatcat cggcctgaat gccatgcccc ccaaaggcgg
     2341 caatcccgat ctggacgatg tcgaaatagc ccgtaccgtg gtgtatatgg ccaaccagtc
     2401 gggcgccagg ttcaaggagc cggaagcgcc tgctcctgcg gaggctcccc cagacaacaa
     2461 ataagagcca ctctaaagcc ggaccgcctg ccaccggaac ggttttgcgc acggcaagcg
     2521 ctgatcgttt atcattaccg gttttgctta tattaccagg agacgcgcga tgctcaaaca
     2581 catcgtcatg tggacactga aagagcatgc cgaaggcgcc gacaaggcta ccaacgcgcg
     2641 caggatgaaa gaactgctcg acggctgcaa gggcctggtg ccgggcatgc tggcttttga
     2701 gaccgcgctg gcgcagcccg gtctggaagc gacttgtgat gtcgtgctgt atgccgaatt
     2761 tacatccagg gcagcgctgg acgcctatca ggaccatccc gatcacgtcg cgatcaagcc
     2821 gttcatcggc gcgattcgcg aagctcgcca atgcatggat tacccaatct gagtagtgcg
     2881 cacgacatcg atggcatcat catgagtcat tccctatctg aaatttttat caatggcaat
     2941 ccctacctgg aaagcctcgg cgtcgaggtg ctggaatatg gcgatggcaa ggcttccatg
     3001 gcgctgctgc tgcgcccgga attcatgaac ggctggcgcg tcgtgcaggg tggtatttcc
     3061 atgacgctga tggatgtcgc gatgggtctg gcgatgcgca cggcgattcc cgatgccagg
     3121 gcggcagcga cggtcgatat gaacgccagc tttttgcagc ccgccggtcg ggccggtgaa
     3181 accctcgtgg cgcaaggcct ggtctaccat cgttcggcga ccatgtgttt ttgcgaagcg
     3241 cagctgtgga acggcgatac gctggttgcc agggccatgg gtacgttcaa ggcgctcaag
     3301 tgtgtcggca tgccgggaaa ctgacaagca gagcaggacg aaaccagaaa gacataaccg
     3361 aatatggatt acagcatccg catcgccgcc tgggctgaac tgcgtgcaga cgccatgctg
     3421 attcgcaacg aagtcttcgt ggccgagcag cgggttcccg ctgaactgga gatggacgat
     3481 atggacgccg tctgcgcgca tgccatcgct tacgacgcag ccggcatgcc gcttgctacc
     3541 ggtcgtttgc tgccagacgg ccatatcggc cgtctggcgg tgcgcagtcg tcgccgcggt
     3601 cagggaatag gcggcgccgt gctgcaggcg ttgatggcgg tagcgcgtca gcgcggggat
     3661 cgcgaagtcg tcctgaacgc gcaaacgcac gcggcgccgt tttacggcag ccatggtttt
     3721 gcgcgggaag gcgaagaatt catggaggcc ggtattccgc acatccggat gcgcgcatgg
     3781 ctggcatgaa cagcagcttc gatcctgcgc gtcgcgcggg gacgttgcac ccgcagtgac
     3841 agccggcggc gatggcaatg ccggccgcac tacctatttc ctgaaataac agagcatgaa
     3901 gcaatgagag acacgcatgg ctaagacgcc gttttacaaa tcactttatt ttcaggtgtt
     3961 gatggcaatt gccatcggcg tattgcttgg tcatttctat ccgcatgccg gcgaggcgat
     4021 gaagccgttt ggcgatggtt ttatcaaact gatcaagatg atcattgcgc cggtcatctt
     4081 ctgtactgtg gtgctgggta ttgccggcat ggaagacatg aaaggggtcg gcaagaccgg
     4141 cggtctggcg ctgctgtact ttgaaatcgt cagcacgctg gcactggttg tcggcctggt
     4201 gctggtcaac gtgctgcagc ccggcgtcgg catgaacgtc gacccgcata ccatcgacgc
     4261 cggaggcatc gctgcctata ccggcccggg caagatgagc agtacgacgg atttcatcat
     4321 gaacgtcatc ccggtgagta tggtcgatgc atttgccaag ggcgatgtgc tacaggtgtt
     4381 actgatctcg gtgctgttcg gttttgcgct gcatcgtttc ggcggtcgcg gcacgctggt
     4441 tttcgactgc atcgaaaagt tttctcacgt cctgttcgcc atggtcggcg ccatcatgaa
     4501 agcggctccg atcggcgctt ttggcgccat ggcctttacc atcggcaagt acggcgtcga
     4561 ttcgctgctg tcgctgggca agctgatggg gactttttac ctgacctgcc tgattttcgt
     4621 gtttgtcgtg ctgggcgcca tcgcgcgtct gcacggtttc agtgtgtgga agttcatcaa
     4681 gtacatcaag gaagaattgc tgatcgtgct gggtacttct tcctcggagt cggtgttgcc
     4741 gcgcatgctg gtcaagatgg aaaacctggg cgtcaa
//
LOCUS       DASRDH010000210         5527 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_15726,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000210 DASRDH010000000
VERSION     DASRDH010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 5527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5527
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15726"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..567)
                     /locus_tag="VFA14_04785"
     CDS             complement(<1..567)
                     /locus_tag="VFA14_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HZS80725.1"
                     /translation="MRELHQSRSFVWTLLIVFLLVWFTTLGGRALVPTDEGRYAEMAR
                     EMVTTQDWITTRLNGIKYFEKPPLQTWMNAITFALFGLGEWQARLWTGLCGLFGIGLV
                     AYTGRRVFNQRVGFYAPLVLASSLFWAGMGHTNTLDMGLAAMMTLSMCALLLAQRADA
                     GRDEQRNGMLLCWAGMALAVLSKGLIGIV"
     gene            complement(567..950)
                     /locus_tag="VFA14_04790"
     CDS             complement(567..950)
                     /locus_tag="VFA14_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016833903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HZS80726.1"
                     /translation="MKLHSTATQQYQTITAYDENGIEINAVRFTHSLLVLPETPPVVW
                     PVTSFEQLSTEHFIQIDAALPDVVILGTGRRQRFAHPRLTSVLGARRVGVECMDNQAA
                     CRTYNILMAEGRKVALALILDEKAA"
     gene            1155..2384
                     /locus_tag="VFA14_04795"
     CDS             1155..2384
                     /locus_tag="VFA14_04795"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HZS80727.1"
                     /translation="MRPIQKSKKLADVCYDIRGPVLEKARQMEEDGHKIIKLNIGNLA
                     AFGFDAPDEIVQDMIRNMNNASGYTDSKGLFAPRKAIMHYTQQKHIQGVAIDDIYLGN
                     GASELMVMSVNALLNSGDEVLLPSPDYPLWTAAVSLSGGTPVHYVCDEQAGWQPDIDD
                     IRKKITPHTKAIVVINPNNPTGALYTTEVLKEIVEIARQNQLIVLADEIYDKVLYDGN
                     THVSLAALADDVLFITFNGLSKNYRSCGYRAGWMVVSGEKKHARDYIEGLNMLASMRL
                     CANAPGQYAIQTALGGYQSINDLVAPGGRLARQRDLAYQLLTGIPGVSCVKPKSALYM
                     FPRLDPSVYPIEDDQKFAYELLAEEKVLIVQGTGFHCATPDHFRVVFLPNTDDLTESM
                     GRIARFLEGYRRRHGTD"
     gene            2432..3742
                     /locus_tag="VFA14_04800"
     CDS             2432..3742
                     /locus_tag="VFA14_04800"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018007695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HZS80728.1"
                     /translation="MKSIKVGLLGIGTVGSGTFNVLQRNQEEIARRAGRGIEITMVAD
                     LNTARATELTHGRVKVVNDANLVVTDPDIDIVIELIGGHGLARELVLQAIAHGKHVVT
                     ANKALIATHGNMIFRAAQEKGVMVAFEAAVAGGIPIIKALREGLTANRIQWIAGIING
                     TTNFILSEMRDKGLDFDAVLKEAQRLGYAEADPTFDIEGIDAAHKLTIMASIAFGIPM
                     QFDKAYVEGITRLQAADIRYAERLGYRIKLLGMTRQTADGIELRVHPTLIPADCLIAN
                     VEGAMNAVMVRGDAVGETLYYGKGAGSEPTASAVIADLVDITRLATADPEHRVPYLAF
                     QPHAMASTPVLTMQDVITSYYLRMRVTDRPGVLADVTRILADSSISIDAMLQKEASEA
                     EMQADIIMLTHQTLERNIEAAIARIEALPTVVDSVMKIRLEALN"
     gene            3840..3915
                     /locus_tag="VFA14_04805"
     tRNA            3840..3915
                     /locus_tag="VFA14_04805"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3873..3875,aa:Lys,seq:ctt)
     gene            complement(3988..4290)
                     /locus_tag="VFA14_04810"
     CDS             complement(3988..4290)
                     /locus_tag="VFA14_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009910417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HZS80729.1"
                     /translation="MQSLALICNERGEALTAFALRFRFENAREAAAKKAHAQKRNELA
                     VAIEAFQFRDLRAKAGTDKEESAGMAAAKDQLGHADEQMTRRYVRHRKGKLVTPTC"
     gene            complement(4383..4667)
                     /locus_tag="VFA14_04815"
     CDS             complement(4383..4667)
                     /locus_tag="VFA14_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS80730.1"
                     /translation="MDRIEEAIKADTPMEKVFPRLMTLATNVEGEGPTIRVRITRNED
                     ASAIRYVSGDDPEGADPEGRQQDETRLQEGLVSDRPLVNIEPIHIRQYLD"
BASE COUNT         1152 a         1602 c         1536 g         1237 t
ORIGIN      
        1 tacgataccg atcagtccct tggacaagac cgccagcgcc atgccggccc agcacagcag
       61 catgccgttg cgctgctcat cgcggccggc gtccgcgcgc tgcgccagca gcagggcgca
      121 catgctgagc gtcatcatcg cggccaggcc catgtccagc gtattcgtgt gacccatgcc
      181 ggcccagaac aggctcgatg ccagtaccag cggtgcgtag aagccgacgc gctgattgaa
      241 cacacggcgg ccggtatagg ccaccaggcc gatgccgaac agcccgcaca gacctgtcca
      301 tagacgcgcc tgccactcgc cgagaccgaa cagtgcgaag gtgatggcgt tcatccaggt
      361 ttgcagcggc ggtttttcga agtacttgat gccgttgagg cgggtggtga tccagtcctg
      421 cgtcgttacc atctcgcgtg ccatttcggc atagcggcct tcatcggtcg gcaccagtgc
      481 gcgtcccccc agcgtggtga accagaccag caggaaaaca atcagtagcg tccagacgaa
      541 gcttctggat tggtgcagtt cgcgcatcag gcggctttct cgtcgagaat gagcgccagt
      601 gcgactttgc ggccttccgc catcaggatg ttgtaagtcc ggcaagcggc ctggttgtcc
      661 atgcattcga caccgacgcg ccgtgccccc aggacgctgg tcaggcgcgg atgcgcgaag
      721 cgctggcgcc ggccggtgcc gagaatgacg acatccggca aggctgcatc gatctggatg
      781 aaatgctcgg tgctcaattg ctcgaaggac gtcaccggcc agacgactgg cggcgtttcc
      841 ggcaatacca gcagactgtg cgtgaagcga acggcgttga tctcgatgcc gttttcgtca
      901 taggcggtga tggtctggta ttgctgggtt gctgtggagt ggagcttcat ggcgcgctat
      961 gtcgaattgc cttcatggct gggcggattg cggcaaccaa gcctcaaagt gctgcattgt
     1021 agcgctttct tgttcggtag caggcatact cattgataga atgcaaggct ttcggcaaaa
     1081 tagcccgctc ctgccgattt tggcggcgtt gtcggactgg cctgtctgac ccactcaaag
     1141 aagggatttg cactgtgcga ccgattcaaa aatcgaaaaa actggctgac gtctgttacg
     1201 acatccgtgg acccgtgctg gaaaaagcgc ggcagatgga agaagacggt cacaagatca
     1261 tcaaactgaa catcggcaac ctggccgcgt tcggtttcga cgcgcctgac gaaatcgtgc
     1321 aggacatgat ccgcaacatg aacaatgcct cgggctatac cgattccaag ggcttgttcg
     1381 cgccgcgcaa ggcgatcatg cattacacgc agcaaaaaca tatccagggg gtcgccatcg
     1441 atgacatcta cctcggcaac ggcgcttccg agctgatggt catgagcgtc aacgcgttgc
     1501 tcaacagcgg cgacgaggtt ctgctgccgt cacctgatta tccattgtgg acagccgccg
     1561 tcagcctgtc cggcggcacg cccgtgcatt atgtctgtga cgagcaggcc ggctggcagc
     1621 cggatatcga tgacattagg aaaaaaatca ctcctcatac caaggccatc gtcgtcatca
     1681 acccgaacaa tccgaccggc gccctgtaca ccacggaagt actcaaggaa atcgtcgaaa
     1741 tcgcgcgcca gaaccagttg atcgttctgg ctgacgaaat ctacgataaa gtcctgtacg
     1801 atggcaacac gcatgtctcg ttggctgcac tggccgacga cgtgttgttc atcactttta
     1861 acggtctgtc caagaactat cgttcctgtg gttaccgcgc cggttggatg gtggtctcgg
     1921 gcgagaaaaa acatgccagg gattatatcg agggcctcaa tatgctggca tcgatgcgct
     1981 tgtgtgccaa tgcgccggga caatatgcca tccagacagc cctgggcggc taccagagca
     2041 tcaatgacct ggtggcgcct ggcggccgtc tggccaggca gcgcgatctg gcgtaccaac
     2101 tgttgaccgg gattccgggg gtgagctgtg tcaagccgaa gtcagcgcta tacatgttcc
     2161 cgcgcctgga cccatcggtg tatccgatcg aggatgatca gaaattcgcc tacgaattgt
     2221 tggccgaaga aaaagtgttg atcgtacagg gcaccggctt ccattgcgcg acgcccgatc
     2281 atttccgggt ggtattcctg cccaatactg acgacctgac agaatcgatg gggcgcatcg
     2341 cgcgtttcct cgaagggtat cgccgccggc atggcactga ctgatttcgg ccttactatt
     2401 ttttgcattt cctgttcacc acgaagacat tatgaaatcc atcaaagtag gtttgctcgg
     2461 catcggcacc gtgggttccg gtactttcaa tgtattgcag cgcaaccagg aagaaattgc
     2521 gcggcgtgcg gggcgcggta ttgaaattac catggtggcc gatctgaaca cggcgcgcgc
     2581 caccgagctg acccatggac gggtcaaggt agtcaacgat gccaatctgg tcgtcaccga
     2641 tccggacatc gatatcgtca tcgaactgat cggtggccat ggcctcgcca gggaactggt
     2701 tttgcaggcg attgcccatg gcaagcacgt ggtcaccgcc aacaaggcgc tgattgccac
     2761 gcacggcaac atgattttca gggctgcgca ggaaaaaggc gtcatggtcg cattcgaagc
     2821 cgctgtcgcc ggtggcattc ccatcatcaa ggcactgcgc gaaggtctga ccgccaaccg
     2881 catccagtgg attgccggca tcatcaacgg cactaccaat ttcatcctgt ccgagatgcg
     2941 tgacaagggg ttggacttcg atgccgtact caaggaagcg caacgtctgg gttatgcgga
     3001 agccgatccg accttcgaca tcgaaggcat cgatgccgcc cacaagctga ccatcatggc
     3061 ttcgatcgcc ttcggcatcc cgatgcagtt cgacaaggcg tatgtggaag gcatcaccag
     3121 gttgcaggcg gccgacatcc gttatgcgga acgactcggt tatcgcatca aattgctggg
     3181 catgacccgt caaaccgccg atggcatcga gttgcgcgtg catccgaccc tgattccagc
     3241 ggactgtctg atcgccaatg tcgaaggcgc gatgaatgcc gtcatggtac gcggcgacgc
     3301 tgtcggcgaa acgctgtact acggcaaagg cgccggttcc gaaccgaccg catcggccgt
     3361 catcgccgat ctggtcgata tcacccgttt ggccactgcc gatccggaac accgcgtgcc
     3421 ttatctggcg ttccagccgc atgccatggc cagcacgccg gtgctgacca tgcaggatgt
     3481 tatcaccagc tactacctgc gcatgcgggt gactgacagg ccgggtgtgc tggccgacgt
     3541 cacgcgtatt ctggcggact cgtcgatttc catcgacgcc atgctgcaaa aagaagcatc
     3601 ggaagcagag atgcaggccg acatcatcat gctcacgcac cagactctgg agcgcaacat
     3661 cgaggcggca atcgccagga tcgaagcttt gccgacggtc gtggacagtg tcatgaagat
     3721 tcgcttggaa gcgttgaatt aggtgtctag tcccggcatt tgatggagca gcccattctt
     3781 tcaaaaagga cttccaggtt tgaaaataat caggttataa tcacgcctct tctttatggg
     3841 gggcgttagc tcagttggta gagcagcgga ctcttaatcc gtaggtcgag tgttcgagcc
     3901 actcacgccc caccagtata tataagggtt tgcggggatt tcaaggtatc atctctgttc
     3961 cgcaaaactg ggtttgttcc gcaattccta gcacgtcggt gtcaccaact tgccttttcg
     4021 gtgccgaaca taccggcgcg tcatctgttc gtcggcatgt ccaagctgat ctttggctgc
     4081 tgccatcccc gccgattctt ccttatcagt gccagccttg gcgcgcaggt cgcggaactg
     4141 gaacgcttcg atagcaacag ctagctcgtt ccgcttctgc gcatgcgcct tcttcgccgc
     4201 tgcctcgcgt gcattctcaa accggaacct gagtgcgaag gcggtcagcg cctctcctcg
     4261 ctcattacag atcagcgcga gcgactgcac tatcccactc gtcgcaggcc gcaccttgag
     4321 cctctcgacc cttgtgctgg tttacccttt tccttcaagc gctggacagc atcctgcacg
     4381 cgtcagtcga gatactgccg gatgtggatc ggttcaatat tgaccagtgg tcgatcgctg
     4441 actagtcctt cttgtaaacg tgtttcgtct tgctgacgcc cttcgggatc cgcgccctca
     4501 ggatcatcgc cggacacgta gcgaatcgcg gaagcatctt cgtttcgggt gatccggaca
     4561 cgaatcgtcg gaccttcccc ttccacgttt gtcgctaggg tcatcaaccg cgggaacacc
     4621 ttctccattg gcgtgtccgc tttgatggcc tcttcaattc ggtccaagtc tcgtttggaa
     4681 accagccacc tctgtgctga catgggcttc catcgccaac aacgccgtga ttcggcctct
     4741 ggcttcgtct cgcgctcttc gtcacccaac ctttggactg agcgatgttc aatgccttct
     4801 taatgccgat ggcggtgccc ttctcgccga atatgttctg ccccttctgg aacagcagcg
     4861 ccttggtcaa catttcgctg gcgtgctgga gcataagcag aatcgtctcc agcctcccgt
     4921 cgtcgtggtt gttgaacgct tccagcccac gtttcaacga tgcaatggcc ttggctttca
     4981 gtgtcttagc ttcccgctta agcctcacga ctacccccat ctttgtctag cgtcgggcgc
     5041 atggtgctgt aaatctgtct gtagctgatg acgccgtcac ctacgcagac ctcgacgcag
     5101 cgactgcgca acgcattttc agcgtcgcag ctgacgatca gcgagagata gacaagcttc
     5161 gcgctctaca aggaacaggg ggccgtatag tttgcgatcg ctgaagttag cgattgattg
     5221 gaggattgca ttgtctacta ggaacgcatg cgccatgtta ttgcggtatt caaccacgct
     5281 ctctaggaaa tgaataaaat cgggtcacaa acctttatct tttagttcgt ttttcgtttt
     5341 cccgagagtc cagaactcca tgtcttcagt aggcacactt gaattgaatt gccccgcttt
     5401 tcctaggcgc tttttatcct cacaataggt gcaaggagaa atgtgtaatg tccataagca
     5461 attcactgaa gaattccgtc aagaagccat ccgtcagatt gttgaccgat ctcgccacgt
     5521 ggccgag
//
LOCUS       DASRDH010000211         8252 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1068352,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000211 DASRDH010000000
VERSION     DASRDH010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 8252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8252
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1068352"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            209..547
                     /locus_tag="VFA14_04820"
     CDS             209..547
                     /locus_tag="VFA14_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019922031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HZS80731.1"
                     /translation="MKQVTAVIKPFKLDEVRESLAEVGVAGLTVTEIKGFGRQKGHTE
                     LYRGAEYVVDFLPKVKIEVVVDDSVVEPVVDAIIKAARTGKIGDGKIFVQEVEQVIRI
                     RTGETGAEAV"
     gene            complement(642..845)
                     /locus_tag="VFA14_04825"
     CDS             complement(642..845)
                     /locus_tag="VFA14_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HZS80732.1"
                     /translation="MATGTVKWFNDSKGFGFITPDDGGEDLFAHFSAIQMNGFKTLKE
                     GQKVQFEVTQGPKGKQASNIQAP"
     gene            1190..1504
                     /gene="clpS"
                     /locus_tag="VFA14_04830"
     CDS             1190..1504
                     /gene="clpS"
                     /locus_tag="VFA14_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008644942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HZS80733.1"
                     /translation="MATQHDNERATVLVRQEQKLKSPPMYQVSLLNDDYTPMEFVVAV
                     IREYFNKDRETAMQIMLRIHHDGKGMCGVYPKDIASTKVERVLTHARRAGHPLQCMME
                     EA"
     gene            1501..3804
                     /gene="clpA"
                     /locus_tag="VFA14_04835"
     CDS             1501..3804
                     /gene="clpA"
                     /locus_tag="VFA14_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HZS80734.1"
                     /translation="MIAQELEVSLHMAFVEARQSRHEFITVEHLLLALLDNPSAAEVL
                     RACAANIDDLRKTLTNFITDNTPTIPGINEVDTQPTLGFQRVIQRAIMHVQSASNGKK
                     EVTGANVLVAIFGEKDSHAVYYLHQQGVTRLDAVNFISHGVRKDQQADAQKAPEGAEE
                     AQAEGQQRESPLDQFTQNLNKAAIEGRIDPLIGRDAEVERVIQTLCRRRKNNPLLVGE
                     AGVGKTAIAEGLAWSITQGDAPEILKNAVVYSLDMGALLAGTKYRGDFEQRLKAVLKQ
                     LKDSQNSILFIDEIHTIIGAGSASGGTLDASNLLKPALSSGQLKCIGATTYTEFRNVF
                     EKDHALSRRFQKIDVNEPTVEQTVQILRGLKSKFEEHHGVKYSVSALTSAAGLAARFI
                     NDRHLPDKAIDIIDEAGAAQRILPKSKQKKIIGKPEIEEIISRIARIPPQSVNQDDRT
                     RLQTIDRDLKNVVFGQDQAIEALASAIKMARAGLGKTDKPIGSFLFSGPTGVGKTEVA
                     KQLAFILGIELIRFDMSEYMERHAVSRMIGAPPGYVGFDQGGLLTEAITRKPHAVLLL
                     DEIEKAHPDIFNILLQVMDHGTLTDNNGRKTDFRNVIIVMTTNAGAESLQKRSIGFTD
                     KKEAGDEMADIKRMFTPEFRNRLDGIISFRALDEAVILRVVDKFLMQLEEQLHEKKVE
                     AVFADDLRKFLAKNGFDPLMGARPMSRLIQDMIRKALADELLFGRLVNGGRVTVELDD
                     KDQVKLEFPEGDPTPPPEEPQERLEIE"
     gene            4141..5103
                     /locus_tag="VFA14_04840"
     CDS             4141..5103
                     /locus_tag="VFA14_04840"
                     /inference="COORDINATES: protein motif:HMM:TIGR01413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HZS80735.1"
                     /translation="MAIPQTMLSIVARDEVHLQFALKRNINIKQLFNRLGKLWEPEIT
                     GITDPTLDRDHELTGGTANVVLGFRPELWRKVNPADVPDNLHSFDKDIVGINGMTAPA
                     TQQDIWIWITQTNAATLYDSMAKAVALLHPFAELVSEQKCFPYHSNVTFDGFADGVAN
                     PSPFRANSVAIIPDGEKGAGSSTVLVQKWRMDVEKLRSLSVPEAEKMWGRTKAGNHEL
                     SPLPEDSHVSRNQFRRNGEEIDIVRRNANYGNAREAGIMFIGFCKDISVTMGMLEQMY
                     GVGADGVMKMDKMLNFATALSSAIYFVPSLDALEKAGISPADPE"
     gene            complement(5147..7069)
                     /locus_tag="VFA14_04845"
     CDS             complement(5147..7069)
                     /locus_tag="VFA14_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HZS80736.1"
                     /translation="MLEFIRNHRRLMQFLLLLFIFPSFAFFGLQSYGGFGDNAHAVAM
                     VDGQTITREELDAAQRQQLEHLRQMAGGRIDAKVFDTPQVRRDVLDSLIAQRAMSAEV
                     ARDRLTVTDQALQRSILQIPGLLKADGSFDKDQYLALLAAQGLTVDSFQARLRRDMGL
                     RQLNAAIQGTAFAPKTVSSHLSGLNAQERLVQEISFKAASYAGQVEVSDEMLKAYYDR
                     NGSRFDVPEQAGIEYVILDSAAVMAQVSVSDADIKSYYDQNIRQYSTEEQRRASHILL
                     KVEQGASAADKAEVKAKADALVMRLRRNPGDFARLARADSQDPGSREQGGDLGFFGKG
                     AMLKPFENAVYQLRQGEISDPVESDFGYHIIELTGIKPGSVKPLETVRDEIADTIGKQ
                     MALRKYAELAEIFNNTIYEQGDSLKPVADKLKLKINTASGVTRWSNLALAPDTPYNDP
                     KFLAALFTDDTIKGRHNTEAVQIAPNTLIAGRIVDYRPVTRRAFDDVKSMVRELVMRD
                     EEMALAKKAGEAKLVALKARDDATGFGNTMVVSRMKTEHWNQAAFLAVMRVDTASLPA
                     YTGVELPGQGYSVFRVTGVVQPATPDIARRKAEQEQITHMLAEQEMLSYIDVLKEKAK
                     TRILQAGQFADAPDEQ"
     gene            complement(7197..8087)
                     /gene="argB"
                     /locus_tag="VFA14_04850"
     CDS             complement(7197..8087)
                     /gene="argB"
                     /locus_tag="VFA14_04850"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HZS80737.1"
                     /translation="MTDSTNVAPQIKAEILAEALPYIRKFHGKTIVVKYGGNAMTKER
                     LKHSFARDVILLKLVGMNPVVVHGGGPQIDNALKKIGKQGSFVQGMRITDEETMEVVE
                     WVLGGEVQQDIVMLINHYGGQAVGLTGKDGGLIRARKMRMPDRENPGRLLDIGFVGEI
                     EAINPAVVKALQDDAFIPVISPIGFGDDGQAYNINADVVAGKIAEILHAEKLIMMTNI
                     AGVQDRQGNLLTDLSAREINAMFEDGSISGGMLPKISSALDAARSGVNTVHIIDGRIE
                     HSLLLEILTEQAFGTMIRSH"
BASE COUNT         2000 a         2256 c         2186 g         1810 t
ORIGIN      
        1 aaatcgaagc tgccggttat cacctggaag acatcgaacg cattacccgt ctgatcagaa
       61 tcaacgagta taagcgccgc caggcgccgg tcggtatccg cgttacgcat cgcgcattcg
      121 gacgcgactg gcgttacccg atcacttccc agtttcgcga gtaatgagaa tgccatgttt
      181 tttcgacata tgatatgagg aagctttcat gaagcaagtc actgctgtca tcaaaccgtt
      241 caagctggac gaagtgcgcg agtcgctggc ggaagtgggt gttgccggtc tcactgtgac
      301 ggaaatcaag ggctttggtc gtcaaaaagg ccataccgag ctatatcgcg gcgccgaata
      361 tgtggttgat tttctgccca aagtcaaaat cgaagtcgtc gtcgacgaca gcgtggtcga
      421 gccggtcgtc gatgcgatca tcaaggcagc gcgtaccggc aagatcggcg atggcaagat
      481 ttttgtgcag gaagttgaac aagtcatccg tattcgcacc ggcgaaacag gcgcggaagc
      541 ggtttgatga cgattttgct ccttgcggcg tgacaaggca taaaaaaacc cccctgcagg
      601 aacagggggg tggttagcag gtgtttcgtt tcaacacgaa atcagggggc ctgaatattg
      661 gaagcctgct tgcctttggg gccttgcgtg acttcaaact gaactttttg accttctttg
      721 agcgttttga agccgttcat ttgaatcgcc gagaaatgcg cgaataaatc ctcgccgcca
      781 tcatccggag tgataaagcc aaagcccttg gaatcgttga accacttgac tgtacctgtt
      841 gccataaaag catctttcaa tgaaaaaaaa tcacacgagc cgttaaagca agaagcctcg
      901 cgagatcgaa acgtccgcag ccctccttaa acctgccccc ctttgattaa caagcagtaa
      961 ttacttactt atcaacgaat tccattgttc tcgctaaaaa tacaaaagtc aagctgtttt
     1021 cagtggaatg attaagcttt tgttcgtgtg atgggcggga aagctcttga tttagggtag
     1081 accttgtctg cggacgatca ggaaagcgcg taatatttaa cggaatccag ataattttcc
     1141 atcattgtgc attgcacaac tcattcattg atcgttttag agtagataca tggcaaccca
     1201 gcacgataat gagcgcgcta cagtcctggt gcggcaggag cagaagctca aatcgcctcc
     1261 tatgtaccag gtatcattgc tgaacgatga ctatacgccg atggaattcg tcgtcgcggt
     1321 cattcgggag tacttcaaca aggaccgtga aacggcgatg cagatcatgc tcaggattca
     1381 ccatgatggg aaaggtatgt gtggtgtata ccccaaagat attgcatcca caaaagttga
     1441 gcgcgtatta acccatgcca ggagagcagg tcaccccctg cagtgcatga tggaggaagc
     1501 atgattgcgc aggagctcga agtcagtttg cacatggcat tcgttgaagc caggcaatcc
     1561 cgtcatgaat ttattaccgt agagcacttg cttctcgctt tgctggataa cccttcggcg
     1621 gcggaagtgc tgcgcgcctg cgcggccaat atcgacgacc tgcgcaagac cctgacgaat
     1681 ttcattactg acaacacgcc aacgattccc ggtatcaatg aagtcgacac acaacccacg
     1741 ttaggcttcc agcgtgtgat tcagcgcgcg atcatgcatg tacagtcggc ttccaacggc
     1801 aagaaagagg taactggcgc caacgtgctg gtcgctatct tcggagaaaa ggattcgcac
     1861 gcggtctatt atctgcatca gcaaggcgtt acccgtctgg atgccgtcaa ttttatttct
     1921 catggtgtgc gcaaggatca gcaagctgat gcgcaaaaag caccggaagg agcggaagag
     1981 gcacaggccg aaggccagca gagagaaagc cctctcgatc agttcacaca aaatctgaac
     2041 aaggccgcta ttgaaggcag aatcgatcca ctgatcggtc gtgatgccga agtcgaacgc
     2101 gtgattcaaa ccctgtgccg tcgccgcaaa aataatccgt tgctggttgg cgaagccggt
     2161 gtcggcaaga cagcgattgc cgagggcctg gcatggagta ttacccaggg cgacgcgccg
     2221 gaaatcctca agaacgccgt cgtctattcg ctcgacatgg gcgcattgct ggccggcacc
     2281 aagtatcgcg gtgatttcga gcagcgtctg aaagcggttc tcaagcaatt gaaggatagc
     2341 cagaatagca ttcttttcat tgacgagatc cataccatca tcggcgctgg ttctgcctcg
     2401 ggaggcacgc tggatgcgtc caatctgctc aaaccagcat tatcgagcgg tcagttaaag
     2461 tgtataggtg cgacgaccta taccgaattt cgtaatgtct ttgaaaaaga tcatgctttg
     2521 tcacgtcgtt tccagaaaat cgacgtcaac gaaccaaccg tcgagcaaac cgtacaaatc
     2581 ctgcgcggtt tgaagtcgaa attcgaagag catcatggcg tcaaatattc cgtttcagca
     2641 ttgacttccg ctgccgggct ggccgcgcgt ttcatcaatg accgtcactt gccggacaag
     2701 gctatcgaca tcattgacga agccggcgcg gcgcaacgca tcctgccgaa gtcgaagcaa
     2761 aagaagatca tcggtaaacc tgagatcgag gaaatcattt ccaggatcgc gcgcattccg
     2821 ccacaatcag tcaatcagga cgaccgcacc aggctacaaa cgatagaccg tgatctgaag
     2881 aatgtcgtgt tcggccagga ccaggcgatc gaagcactgg cgtcggccat caagatggcg
     2941 cgcgccggcc taggcaaaac cgacaaaccg atcggctcct tcctgttttc cggtccgacc
     3001 ggcgtcggca agaccgaagt ggcgaagcaa ttggcattca tcctcggtat cgagctgatc
     3061 cgtttcgata tgtctgaata catggagcgc catgcagtga gccgtatgat cggcgcgccg
     3121 ccgggctatg tcggtttcga ccagggtggc ctgctgaccg aagcgatcac cagaaagccg
     3181 cacgctgtgc tgctgctcga tgaaatcgaa aaagcgcatc cagacatctt caacattctg
     3241 ttgcaggtga tggatcatgg caccttgacc gacaacaatg gccgcaagac cgattttcgt
     3301 aacgtcatca ttgtgatgac cacaaatgcc ggcgcagaaa gcctgcagaa gcgctccatc
     3361 ggtttcaccg acaaaaaaga agccggtgac gagatggcag atatcaagcg catgttcaca
     3421 ccggagttcc gtaaccgtct ggatgggatc atcagtttcc gcgcattgga tgaagcagtc
     3481 atcttgcgcg tggtcgataa attcctgatg caactggaag aacaactgca cgagaaaaag
     3541 gtggaagctg tcttcgctga cgacctgcgc aaattcctgg cgaagaacgg cttcgatccg
     3601 ctgatgggcg cgcgtccaat gtcgcgcctg atccaggata tgatccgcaa agctctggcc
     3661 gatgaactgc tgttcggtcg cctggtcaac ggtggccgtg tcaccgttga actggatgac
     3721 aaggatcagg tcaagctgga gtttccagaa ggggatccga caccaccgcc ggaagaaccg
     3781 caagaaagac tggaaatcga ataagcgccg ctgaagccat gttgatgctg ctgacgcagc
     3841 acaaggtgag cggagctgca aaaaaccaga tagaaagccg aagcgactgc cagccggcgc
     3901 ccgctgcctg tgccgggtcg aggtgatgct cattcatggc gcaggccctt cttcatatta
     3961 ccgcgtttgc tctgccggcc gttccttctg gaccgaataa aagcccagaa cacgaagcat
     4021 gtctctggca tgacttgcga cttctggaag gtgaaaaacg gaggacggcc catggcaggc
     4081 agccaatttg gtttaagatc atcgcaggaa atcaagaaat agaaacagga aagaataaat
     4141 atggccatac cacaaacaat gctttctatc gtcgcgcgcg atgaggttca tttacagttt
     4201 gcgttaaaaa gaaatatcaa tatcaaacag cttttcaaca ggctcggcaa gctctgggaa
     4261 ccggaaataa ccggtatcac cgacccaacg ctggaccggg atcatgaact gaccggcggc
     4321 actgcgaatg tcgtccttgg attcaggcct gagttatggc gcaaagtgaa ccccgccgac
     4381 gttcccgaca atctgcattc cttcgataaa gatatcgttg gcatcaacgg catgaccgcg
     4441 ccagcaacac agcaggatat ctggatctgg atcacacaaa cgaacgccgc aacgctctat
     4501 gacagcatgg ctaaagcagt ggcgttgctg catccttttg ccgaactcgt cagcgagcag
     4561 aaatgtttcc cttaccacag caacgtcaca ttcgatggtt ttgcggacgg cgtggcgaat
     4621 cccagtccat ttcgtgccaa cagcgtggcc atcattcccg atggcgagaa gggcgcaggc
     4681 agttccacgg tgctggtcca gaaatggcgt atggatgttg aaaaattaag gagcctgtcc
     4741 gttcccgaag cagaaaaaat gtggggcaga accaaagcag gaaaccatga actgtcgcct
     4801 ttgcctgagg actcccatgt cagcaggaat cagtttagaa ggaatggcga agaaatagac
     4861 atcgtccgca gaaacgccaa ttatggcaat gcacgagagg ctggaatcat gttcatcggc
     4921 ttttgcaaag acatatcggt caccatgggg atgcttgagc aaatgtacgg ggtaggagcc
     4981 gatggcgtca tgaagatgga caaaatgctc aattttgcca cggcgctttc ttctgcgatc
     5041 tattttgtgc ccagtctgga tgcattggag aaagcaggaa tttcacctgc ggatcctgaa
     5101 tgagagcctg tactggtaac cgggaaacac atcgacgccc gcatcgtcac tgttcatccg
     5161 gcgcatctgc aaactgacca gcctgcagga tcctggtctt ggccttttcc ttgagaacat
     5221 cgatgtagga cagcatttcc tgctcggcca gcatatgcgt tatctgctcc tgctcagcct
     5281 tgcggcgagc gatgtcaggc gttgccggct gcaccacccc ggtcacgcgg aacacgctgt
     5341 atccctgccc cggcagttcg acaccggtgt aagcaggcaa actggcggta tcaaccctca
     5401 tcaccgccag aaaagccgcc tgattccagt gctcggtttt catgcgggag acaaccatgg
     5461 tgttaccgaa accggtggcg tcatccctgg ccttcaatgc caccagcttg gcttcgccgg
     5521 cctttttggc cagcgccatt tcttcgtccc gcatcaccag ttcacgcacc atgctcttga
     5581 cgtcgtcgaa cgcccgccgg gtcaccggcc tgtaatcgac gatacggcca gcgatcaatg
     5641 tgttcggcgc gatttgcacc gcttcggtat tgtgcctgcc cttgatagtg tcgtcggtaa
     5701 aaagcgccgc caaaaacttg ggatcgttat acggcgtatc cggcgccaat gcaagattcg
     5761 accagcgcgt cacgccggag gctgtgttga tcttcagctt gagcttgtcg gccaccggct
     5821 tgagactgtc accctgttcg tagatggtgt tgttgaagat ctcggccagc tcggcgtact
     5881 tcctgagcgc catttgcttc ccgatggtgt cagcgatctc atccctgacg gtctccaggg
     5941 gcttgacgct gcctggcttg ataccggtca gctcgatgat gtgataaccg aaatcggact
     6001 cgactggatc actgatttcg ccctgcctga gctggtaaac agcgttttca aacggcttga
     6061 gcatggcgcc cttgccaaag aaaccgaggt cgccgccctg ctccctggaa cccggatcct
     6121 gggagtcggc cctggccagt ctggcgaaat cgcccggatt cctgcgcagc cgcatcacca
     6181 gcgcatctgc cttggccttg acctcagctt tatcggccgc ggacgcgccc tgctcgactt
     6241 tcaacagaat atggctagcg cgacgctgct cttccgtcga gtactggcgt atgttttgat
     6301 cgtagtacga cttgatgtca gcatcagaca cgcttacttg tgccatgacc gcagcgctgt
     6361 cgagaatgac gtattcgatc ccggcctgct ccggcacgtc aaatctgctg ccgttcctgt
     6421 cgtaataggc tttgagcatc tcgtcgctga cctcgacctg acctgcatag gaggctgcct
     6481 tgaaactgat ttcctgcacc aaacgctcct gagcattcag gccggacaaa tgcgaagaaa
     6541 ctgttttcgg tgcgaacgca gtaccttgaa tcgccgcgtt caactgtcgc aagcccatgt
     6601 cacgacgcag acgcgcctgg aaggaatcca cggtcaaacc ttgcgccgcc agcagggcca
     6661 gatactgatc cttgtcaaaa ctaccatccg ccttgagcag gcctggaatc tgcaggatgc
     6721 ttctctgcaa tgcctgatcc gtcactgtca gcctgtcgcg tgccacttcc gcgctcatcg
     6781 cgcgctgggc aatcaaacta tccagcacat cgcggcggac ctgcggcgta tcgaatactt
     6841 tggcgtcgat ccggccaccg gccatctggc gcaaatgctc cagttgctgg cgctgcgcag
     6901 catccagctc ctctctggta atggtctggc catccaccat ggccacggca tgggcattat
     6961 cgccaaagcc accgtagctt tgcaggccaa aaaacgcgaa agacgggaaa ataaacagca
     7021 gcagtagaaa ctgcatcaag cgccgatggt tacgaatgaa ttcaagcatt ggtcagccga
     7081 tcacgaacag gaggagcata tgaaaaaagc gaactcacat tcgccctgga ggatgccgcc
     7141 ctggcaatac ccgcgccatt atacacagac ggccaaggtg aggtccgtca ggacgctcag
     7201 tgcgagcgaa tcatcgtgcc aaaagcctgc tcggtcaata tttccaacaa cagcgaatgc
     7261 tcgatgcgac catcgatgat gtgtacggtg ttgacgccgg acctagcggc atctagcgcc
     7321 gacgaaattt tcggcaacat gccgccggaa atgctaccgt cttcaaacat ggcattgatc
     7381 tcgcgcgccg acagatcagt cagcaaatta ccctgcctgt cctgcacgcc ggcaatgttg
     7441 gtcatcatga tcagcttttc ggcatgcaga atttcggcga tcttgccggc cacgacgtcg
     7501 gcattgatgt tgtaagcctg accatcgtcg ccgaaaccga tcggtgaaat aaccggaata
     7561 aaggcatcat cctgcaaggc tttgaccacc gccgggttga tcgcttcgat ctcgccgacg
     7621 aaaccaatgt cgaggagtcg gcccggattt tccctgtccg gcatgcgcat cttgcgggca
     7681 cggatcaggc cgccatcctt gccggtcagg ccgacggcct ggccgccgta atgattgatc
     7741 agcatgacga tatcctgctg cacttcgccg cccagcaccc actcgaccac ttccatggtt
     7801 tcttcgtcgg tgatgcgcat accctgcacg aaggaaccct gcttgccgat ctttttgagc
     7861 gcattgtcga tttgcgggcc accgccatgt accacaaccg gattcatgcc gaccagcttc
     7921 aacaggatca catcgcgtgc aaaactatgc ttcagacgct cttttgtcat tgcattgcca
     7981 ccatatttga cgacgatggt cttgccgtgg aacttacgaa tgtacggtaa ggcttcagcc
     8041 agaatttcgg ccttgatttg aggagcaacg ttggttgaat cggtcatggc gcacattccg
     8101 ggctgaaaga gatatctgga acgtccgcga ttttacatgg aaaatgcctc accatcgcgg
     8161 cggctgtcat gctttgctga tacaccgcta ctctgaacag gcgcgcattt cctgataggg
     8221 gaagtttgtc ttgaagctgt ctaacgccgc tt
//
LOCUS       DASRDH010000212         5736 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_40875,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000212 DASRDH010000000
VERSION     DASRDH010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 5736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5736
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40875"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            79..1833
                     /locus_tag="VFA14_04855"
     CDS             79..1833
                     /locus_tag="VFA14_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase ATPase subunit family protein"
                     /protein_id="HZS80738.1"
                     /translation="MSVAPPETDHRRIARSLYWQGWRVSSIAEYLGESRSTVNSWKDR
                     DGWADTPVIERIENSLEARMIQLIGKDVKTGGDYKEIDLLGRQVERLARVRKYEGSGK
                     ESDLNPNLERRNAMPKRKPVKNEFDEAQTAQIREAFLDSLFDYQKVWYRNGDKRTRMI
                     LKSRQIGATWYFAREALIDAIETVRNQIFLSASKSQAHVFKQYIIQFAKEACGVELSG
                     DPLVLANGAHLYFLGTNARTAQGYHGNFYFDEFFWTYNFEELNKVASGMALHKQWRKT
                     YFSTPSAMSHQAYPLWSGDVFLQRRPKADRVPFDVSHARLSNGFTGEDKIWRQIVTIL
                     DAQAGGCDLFDIDELRDFEYSPDQFDNLLMCNFIDDSASVFPLTAMQRCMVDSWVDWH
                     DYKPFTERPFGHRPVWIGYDPSLTGDSAGCIVLAPPDRPGGVFRILERHQWRNMDFEA
                     QAEAIRQITWRFNVTYIGIDVTGMGIGVYQIVQKFFLQATRFDYSPEVKSQLVLKAQN
                     VINHQRLQFDAGWNDIAQSFMAIRKMLTASGKHVTYDAGRNRETGHADLAWACMHALA
                     NESLDGELPTSRSTMEMY"
     gene            1833..2879
                     /locus_tag="VFA14_04860"
     CDS             1833..2879
                     /locus_tag="VFA14_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012352234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HZS80739.1"
                     /translation="MSRKHERQAMHSRAAAASEVRKTAAEAFTFGDPTPVMDRREILD
                     YLECWENGRYYETPVNFNGLARSFRASVHHSSAIYVKRNILASTFIPHKLLSMDSFRR
                     WALDYMIFGNGYLEESKNRLGKAMELTPPLAKYMRRGMEDHQYFYVPTSLKEHEFARG
                     SIFHLVEYDIHQEIYGLPEYLSALQSAWLNESATLFRRKYYLNGSHAGFILYLTDSQV
                     DKTDVDALRGALRESKGPGNFRNLFMHAPGGKKDGMQILPVSEVAAKDDFFNIKNVTR
                     DDLLAVHRVPPQLLGVMPNSTGGFGDVERAAKVFAENELKPLQLRFLEVNDWLGEEVV
                     RFTPYALGVQQEKN"
     gene            2879..3106
                     /locus_tag="VFA14_04865"
     CDS             2879..3106
                     /locus_tag="VFA14_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2116 family Zn-ribbon domain-containing
                     protein"
                     /protein_id="HZS80740.1"
                     /translation="MGDHADISDAGIEAAVARGIADVRSKASLQATRHCHFCDAEIGD
                     ALLFCDADCRDDYDQEQAALKRAGRKGYNIL"
     gene            complement(3395..4528)
                     /locus_tag="VFA14_04870"
     CDS             complement(3395..4528)
                     /locus_tag="VFA14_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012467712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HZS80741.1"
                     /translation="MSKIRKIILPTEAEDVRIIAAADADAKRAEARRKKAKENLIKSL
                     AGGDFSNQQTRVAHILNLHPAARNSDVVLALKYWEMFHPEFYNPDGIKPADFFKLDRV
                     PFLVRARAKIQNEYELFQADEKVRRRRRGREDEMREAVLADEAPRQTLCVYSDETGKS
                     EDYVIIGSVWVLNGRAVYDVTKAIKDWQVGSKFARREVHFSAFGKDDLDAVADYLSVI
                     AANREFLSFKLIAINRRNSRRPIEDVVQRLHERMLVQGLRHEIESGRVGVLRHVALTM
                     DEEQSLDRIALADMRDRVEEGIEREHLDGVTLDDRFNTVSSKDSALVQLADVIAGVAN
                     RRLNIKGKRNYKDEIADQVMDALGLKLAEGETPEMDAAVLFWI"
     gene            complement(4512..4742)
                     /locus_tag="VFA14_04875"
     CDS             complement(4512..4742)
                     /locus_tag="VFA14_04875"
                     /inference="COORDINATES: protein motif:HMM:NF016268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HZS80742.1"
                     /translation="MRAEVKNGINATLIDWDIALVWIDNRKDYSEQRECALGVIGDRV
                     YFCAFTRRGRVLRIISLREDNLREAKYYVKNS"
     gene            5292..5645
                     /locus_tag="VFA14_04880"
     CDS             5292..5645
                     /locus_tag="VFA14_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HZS80743.1"
                     /translation="MYDHGTVASKPIEFRGSSLDDLRSFPPPARNEAGHQLDQVQKGQ
                     EPDDWKPMNTVGQGVKEIRIRDAVGAFRVIYVEKFANAVYVLHCFQKKTEKTSRGDLD
                     LASKRYSDLLQELDK"
     gene            5642..>5736
                     /locus_tag="VFA14_04885"
     CDS             5642..>5736
                     /locus_tag="VFA14_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018079665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HZS80744.1"
                     /translation="MSNQRFASVWDAIEDTPEEAENMKLRSVLMTA"
BASE COUNT         1356 a         1533 c         1517 g         1330 t
ORIGIN      
        1 aacgaggcaa ggatgtacat gccacgtgta cattccctgc actctcacgc gcgcgcgatg
       61 tgacacgaca atcggcacat gtccgtcgcg ccacctgaaa ccgaccatcg ccgcatcgcc
      121 cgcagcctgt actggcaggg atggcgtgtt tcgtcgatcg cggaatatct tggtgagagc
      181 cgcagcacgg tgaacagctg gaaagatcgc gacggctggg cggacacgcc tgttatcgag
      241 cgcatcgaga attcgctcga agcgcgcatg attcagctaa tcggcaagga tgtaaaaacc
      301 ggcggcgatt acaaggaaat cgatttgctg ggccgacagg tcgaacggtt ggcgcgtgtg
      361 cgcaaatacg aggggagtgg caaggaatcc gatctcaatc caaatcttga gcgccgcaac
      421 gccatgccga agcgtaaacc ggtcaaaaac gaattcgatg aggcgcagac ggcacagatc
      481 cgggaggcat ttctcgattc gttattcgat tatcagaagg tctggtatcg aaacggcgat
      541 aagcgcacgc ggatgatcct gaagtcacgc cagatcggcg cgacatggta tttcgcacgt
      601 gaagcgctga tcgatgcgat cgagaccgtc cgtaatcaga ttttcctttc cgcgtcgaag
      661 tcgcaggcgc atgtcttcaa gcaatacatc atccagtttg cgaaggaggc ctgtggggtc
      721 gaattgtcag gcgatccgct cgtactcgcc aacggcgcgc atctctactt cctcggcacc
      781 aatgcacgga cggcgcaggg ctaccatggc aatttctatt tcgacgagtt cttctggacg
      841 tacaacttcg aggaattgaa caaagtggcg tcaggcatgg cgctacacaa gcaatggcga
      901 aagacctatt tctctacgcc ttctgcgatg tcgcatcagg cttatccgct atggagcggg
      961 gatgtgtttc tgcagcgccg accgaaggcg gaccgcgttc cgtttgatgt cagccatgca
     1021 cggctttcca atggatttac cggtgaggac aagatctggc gtcagatcgt caccattctc
     1081 gatgcgcagg ctggcggatg cgatctattc gatatcgacg agctgcgcga ctttgaatac
     1141 agcccggatc agttcgacaa cctgttgatg tgcaatttca tcgatgacag tgcatcggtg
     1201 tttccgctga cggcgatgca gcggtgcatg gtggattcat gggttgactg gcacgattac
     1261 aagcccttca ccgagcggcc gttcggccat cgtccggtct ggatcggtta cgacccatcg
     1321 cttaccggag acagcgccgg ctgcatcgtg ctggcgccgc ctgacaggcc tggcggggta
     1381 ttccgcattc ttgagcgcca tcaatggcgc aacatggatt tcgaggcgca ggccgaggcg
     1441 atccggcaga ttacatggcg tttcaacgtc acgtacatcg gcatcgatgt cacgggcatg
     1501 ggcatcggcg tgtatcagat cgtccaaaaa tttttcctgc aagccacgcg cttcgactat
     1561 tcgccggaag tgaaatcgca gctggtgctg aaggcgcaga acgtaatcaa tcaccagcgc
     1621 ctgcagttcg atgccggctg gaatgacata gcacaatcgt tcatggcgat tcgcaagatg
     1681 ctgaccgcca gcggcaagca tgtgacttat gacgctggac ggaatcggga aaccggccat
     1741 gcagatcttg catgggcctg tatgcatgcg ctggctaacg aatcgctgga cggcgaattg
     1801 ccgacgtcca gatccactat ggagatgtac tgatgagtcg aaaacacgag cggcaggcta
     1861 tgcacagccg tgcggcggcg gcatctgaag tgcgaaaaac agcagcggag gcgttcacct
     1921 tcggtgatcc gacgccggtg atggatcgcc gcgagattct ggattatctg gaatgctggg
     1981 agaacggccg gtattacgaa acgccagtga atttcaatgg gctggcgcgg tcgttccggg
     2041 cgagcgtgca tcacagttcg gccatctatg tgaagcgcaa cattctggcg tccacgttca
     2101 ttccgcacaa gctgctgtcg atggattcct tccggcgctg ggcgctcgat tacatgatct
     2161 tcgggaatgg ctatctcgaa gaatcgaaaa accgcctcgg caaggctatg gaactgacac
     2221 caccgctggc gaagtatatg cggcgcggca tggaagacca tcaatatttt tatgttccga
     2281 cgtcgttaaa ggagcatgag tttgcacgcg gctcgatctt ccatttggtc gaatatgaca
     2341 tacatcagga aatctacggc ttgccggaat acttgagtgc gttgcaatcg gcatggctga
     2401 atgaatcggc gacgctgttc cggcgcaagt attacctgaa cggttcacat gccggtttca
     2461 tcctgtatct caccgattcc caagtcgaca agaccgacgt cgatgcgctg cgcggcgcac
     2521 tgagggaaag caaggggcct ggcaacttcc gcaatctgtt catgcatgcg ccgggcggaa
     2581 aaaaagacgg catgcagatc ctgccggtct cggaagtcgc tgccaaggat gattttttca
     2641 acatcaaaaa cgtgacacgt gacgatctgc ttgcggtaca tcgtgtaccg ccgcagcttc
     2701 tgggcgtgat gccgaacagt acgggtggat tcggtgacgt cgagcgtgcc gccaaggttt
     2761 ttgcagaaaa cgaactgaag ccgctccagc tgcgcttcct agaagtgaat gattggctcg
     2821 gtgaggaagt ggtgcgtttt acgccctatg cgctgggcgt gcaacaggag aaaaattgat
     2881 gggcgatcac gcagatattt ccgatgcagg tatcgaggca gcagtagcgc gtgggatcgc
     2941 tgatgtgcgt agcaaggcat cgctgcaggc aacaaggcat tgtcatttct gcgatgcgga
     3001 aattggcgac gctctgttgt tctgcgatgc tgattgccgt gacgactacg accaggagca
     3061 ggccgcgctg aagcgcgccg gccgcaaggg ctacaacatc ttgtgactga tggcatattc
     3121 gcatagagca cagttcttca tttgcttgcc gatcagcagc tgtgcaaatg gattctgtag
     3181 ccccctcaaa aaatgaacac tattggcata gtcaatctca tatggttttg actagcaatc
     3241 agccctcatg caagagcctt cacatgtaat tccccgatta ttgagatatt cgatgaacac
     3301 tatgcggtct gcataactct atgatgatgt aggttatatc ctacatcgac ccaagatgtc
     3361 cgcgtgcgat agttcacacg gaagcggcaa ggagctaaat ccagaaaagc acggccgcat
     3421 ccatttcagg cgtctcaccc tctgccaact tcaggcccag agcgtccatc acttgatccg
     3481 cgatctcgtc cttatagtta cgcttcccct tgatattaag ccgacgattc gccacaccgg
     3541 cgattacatc ggcgagctgt actagtgcgc tatcttttga cgacaccgtg ttaaatcggt
     3601 catccagcgt cacaccgtcg agatgttctc gctcaatgcc ctcctccacc cgatcacgca
     3661 tgtccgccaa agcgatgcga tctagcgact gctcctcgtc catcgtcagc gctacgtgcc
     3721 gaagcacgcc aacgcggcca ctctcgattt cgtgacgcaa cccctgcact agcatgcgct
     3781 cgtgcagacg ttgcaccacg tcttcaattg ggcgtcgcga gttacgccta tttatcgcga
     3841 tcagcttgaa actaagaaat tcgcggttcg cagcgataac gctcaaataa tcagccacgg
     3901 catccaaatc gtccttaccg aacgcagaaa aatgaacctc tctcctagcg aatttcgagc
     3961 cgacctgcca gtccttgatg gccttggtca cgtcgtacac agcgcggcca ttcaacaccc
     4021 agaccgagcc aataatgacg taatcctcgc tctttccggt ctcgtcagaa tacacacaca
     4081 gcgtctgtcg aggtgcttca tccgccagaa ccgcctcacg catctcgtct tcacgtcctc
     4141 ttcgtcgacg ccttaccttt tcatcggctt ggaacagctc gtattcgttt tgaattttcg
     4201 ccctagcgcg cacaagaaac ggcacacggt cgagcttgaa gaagtctgcg ggctttatgc
     4261 catccggatt gtagaactca gggtggaaca tctcccagta tttcaatgcg agcaccacgt
     4321 cactgttccg tgcagccgga tgcaagttaa ggatgtgagc gacgcgagtt tgctggttcg
     4381 aaaaatcgcc cccagcaagc gacttaatca gattctcttt ggctttcttc cgacgagctt
     4441 ccgcacgctt ggcatccgca tccgcagcag cgataatcct tacatcttcc gcctcggtcg
     4501 gcaggatgat cttacgaatt tttgacatag tattttgcct ctctcaaatt gtcctcgcgc
     4561 aagctgatga tgcgtagcac tctgcctcgt cgcgtgaacg cgcaaaaata tacacggtca
     4621 ccaatcacac cgagcgcgca ttcgcgctgt tcgctgtagt cctttcgatt atctatccag
     4681 accagcgcga tatcccaatc gatcagcgtc gcattaatgc catttttaac ctcggcgcgc
     4741 agtcagcccc ccaccacgcc tgcccgctag atagggctgt tttatctcat tgtttgtcgt
     4801 agcagaaaac cgcgccagta ttggctttac tggcggctgg gagggaaatt tttttgacgc
     4861 aaaatgacgt gcgcatcgct ttttttgtag ggtgttgagc aggcgatcgg ggaaagagac
     4921 tgaaggctaa ttttcaggtg gggcgtgggg gaaaaggtaa tcttagttat cacccctgaa
     4981 aatcattctc aagtatctga tttataaagg atttcaagat taccttttaa cagttatcca
     5041 gggtaataaa aaggtaatct gtttttaagt tattgattta taaggatttt aaaaactaaa
     5101 aaattacctg atacaacggt aaccacatta ctatcagatt actaaaaaat tacctttttc
     5161 tattttgcaa catctgcatt cagcaaggtt ttccgggcga tccgtcaccg atgttaccaa
     5221 tgttaccttt ttcccacgcc ccattaaatt ttggcagctg ctcccaaaac ccacttgccc
     5281 catataaaat cttgtatgat catggcacgg ttgcttcaaa acccatcgag ttccggggta
     5341 gctcccttga tgatcttcgc tcctttcctc ccccggcaag gaacgaggca ggccatcagc
     5401 ttgatcaggt acagaaagga caagaaccag acgactggaa acccatgaat accgtgggac
     5461 aaggtgtgaa ggagattcgg attcgggatg ctgtcggggc gtttcgagtt atctacgttg
     5521 aaaagttcgc taatgccgtc tatgtgcttc actgcttcca gaagaagacc gagaagacca
     5581 gcagggggga tttggacttg gcatcgaagc gttacagtga cttgttgcag gagctagaca
     5641 aatgagcaac caacgatttg ccagcgtctg ggatgccatc gaggacaccc cggaagaagc
     5701 ggaaaatatg aaactgcgtt ccgtcctcat gacggc
//
LOCUS       DASRDH010000213         3409 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_581681,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000213 DASRDH010000000
VERSION     DASRDH010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 3409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3409
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_581681"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..1612)
                     /gene="lnt"
                     /locus_tag="VFA14_04890"
     CDS             complement(83..1612)
                     /gene="lnt"
                     /locus_tag="VFA14_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HZS80745.1"
                     /translation="MPAGIVNVLAFAPFNIWPIQILTLAWLFHRISSRDAALKHHALS
                     GWLYGFGWAICGMHWLYISMHDYGGMPGWMAALAVCLLAAYLGLYAALAAGLAGWLKQ
                     RWRVAPVILLLLILPALWACSEWLRGWLFTGFPWLTSGYAHNVGPLAGFAPLVGVYGL
                     AWIAAIMAGCLAMMGRRANPCALPAMLTVVLAGAGVALQSVSWTTPAGKPISVRLLQG
                     NIPQEMKFSNEALLSSLMLYDDMIRSAPADLIATPEIAVPLLPQQLPPDYLARLTRFV
                     HSTGSHLALGMPLSNAAGQYSNSVVGFSPDPASAGNAYRYDKQHLVPFGEFIPPGFRW
                     FVDMMHIPLGDMTRGKSLQPPFQVKQQWIMPNICYEDLFGEEIARQIRAATGQAQASI
                     LLNLSNIAWFGHSIALPQHLQISQMRALETGRPMLRSTNTGATAVIGPDGRLLSALQP
                     YTRNVLTASIQGYQGQTPYILLGNSLVLALMLTALVTAWLLSRRRAGFTDEAHSSPEN
                     S"
     gene            complement(1703..2590)
                     /locus_tag="VFA14_04895"
     CDS             complement(1703..2590)
                     /locus_tag="VFA14_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HZS80746.1"
                     /translation="MQDHPSSVKSSDAKPHRSLFERLTAFISPEPENRAELLEVLQDA
                     HARNLIDADALSMIEGVFQVSDLAVRDIMVPRAQMDTIDISNPIESWLPDVLSTAHSR
                     FPAVEGERDKVIGMLLAKDLLRYYTEASFDVRDMLRPAVFIPESKRLNVLLRDFRANH
                     NHMAIVVDEYGGVAGLITIEDVLEQIVGDIEDEYDFDEEEDNILALKDGTNGPRWRVK
                     GLTEIAQFNDMLETALPGTDADTIDSLAAGHLGRIPRKGDVFDIDGIHFEVLRADARQ
                     THTLLVEKLPKTPADGEET"
     gene            complement(2659..2967)
                     /locus_tag="VFA14_04900"
     CDS             complement(2659..2967)
                     /locus_tag="VFA14_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HZS80747.1"
                     /translation="MSLNAVAAGDAKTGKLLVEKYNCASCHGKDFSSSIDPSYPKLAG
                     QHQDYLQHTLMAYRRGNDGMNGRVNAIMSGQVEMLTDQDMQDIAAYLHSLPGSLVLQQ
                     "
     gene            complement(3046..3360)
                     /locus_tag="VFA14_04905"
     CDS             complement(3046..3360)
                     /locus_tag="VFA14_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007878211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HZS80748.1"
                     /translation="MKKLLILLALTGAAGAVSAVDLAGNTGTVNVSMCIGCHGIPGYK
                     ATFPEVYQVPMLGGQSEKYIENALKAYQKGERRHTSMQGIAASLSDQDIAALAAYYAQ
                     QK"
BASE COUNT          693 a          948 c         1063 g          705 t
ORIGIN      
        1 gcttgaggat gatctgttgg aatgtaagca ttgtttgtgg ccgccgctga aggcgggaaa
       61 ggaattgcgg aagccctgaa ttctagctgt tttcagggga gctatgcgct tcgtctgtaa
      121 aaccggcgcg gcggcgcgac agcagccagg ccgtcaccag tgcagtcagc atcagcgcca
      181 gcaccaggct gttgccgagc agaatgtagg gagtttggcc ctgatagcct tgtatcgacg
      241 cggtgagtac attgcgcgtg tagggttgca atgcagacag cagccggccg tccgggccga
      301 ttacggcagt ggcgccggtg ttggtcgaac gcagcatcgg ccggccggtt tcgagcgcgc
      361 gcatctgcga gatttgcaag tgctgcggca gtgcgatgga atgaccgaac caggcgatgt
      421 tggacaggtt cagcagaatg ctggcctgcg cttgtccggt ggcggcgcgg atctgtctgg
      481 cgatttcttc gccgaacaga tcttcgtagc agatattcgg catgatccat tgttgcttga
      541 cctggaaagg cggctgcagg gacttgccgc gcgtcatgtc acccagcggg atgtgcatca
      601 tgtcgacgaa ccagcggaat ccgggcggga tgaattcgcc gaaaggcacc agatgctgtt
      661 tgtcataacg ataagcattg ccggcgctgg ccggatcggg tgaaaaaccg accacactgt
      721 tgctgtactg gccggcagcg ttcgacaagg gcatccccag tgccagatga cttcctgtgc
      781 tatgcacgaa gcgcgtcaga cgcgccagat aatctggcgg taactgttgc ggcagcagtg
      841 gcacggcgat ttccggtgtg gcgatcaggt cggcaggcgc gctacggatc atgtcgtcgt
      901 acagcatcag cgatgacagc agtgcctcat tcgaaaactt catctcctgc gggatatttc
      961 cttgcagcag gcgcaccgaa atcggtttgc cggcgggcgt ggtccagctg accgactgca
     1021 gggccacgcc cgcgccagcc agcacgaccg tcagcatcgc aggcaaggcg caggggttgg
     1081 cgcgccggcc catcatggcc aggcagccgg ccatgatggc agcaatccag gccagaccgt
     1141 agacgccgac cagcggcgcg aaaccagcca gcggcccgac attgtgggca taccccgagg
     1201 tcagccacgg aaaaccggta aacagccagc cgcgcaacca ttccgagcaa gcccataggg
     1261 ccggcagtat cagcagcagc aggatgaccg gcgcgacgcg ccaacgctgc ttgagccagc
     1321 ctgccagtcc tgccgccagc gcggcgtaga gaccaagata ggcagccagc aggcagactg
     1381 ccagcgcggc catccatccc ggcatgccgc cgtagtcatg catgctgatg tacagccagt
     1441 gcatgccgca gatcgcccag ccaaagccgt acagccagcc gctcagggca tggtgtttca
     1501 gcgcagcgtc gcgactactg atccgatgga atagccacgc cagcgtcaga atctggatcg
     1561 gccagatgtt gaagggcgca aatgccagca cattgacgat gccggctggc aaggcgacgg
     1621 ttgacctcca ggcgctcgca gaggcaggcg cgacgttcgg cggggcaggg gagcgggtcg
     1681 caggaaaatt cacgcggaga gtttatgttt cttccccgtc cgccggcgtt ttcggcagtt
     1741 tttccaccag cagcgtatgg gtctggcggg catcggcgcg cagcacttcg aagtgtatgc
     1801 catcgatgtc gaacacatcg cccttgcgcg gtatgcggcc gagatggccg gcggccaggc
     1861 tgtcgatcgt gtcggcgtcg gtgccgggga gggcggtttc cagcatgtca ttgaactggg
     1921 cgatttcggt cagacccttg acacgccagc gcgggccatt ggtgccatcc ttgagggcaa
     1981 ggatattgtc ttcttcttcg tcgaaatcat attcgtcttc gatgtcgccg acgatctgct
     2041 ccagcacgtc ttcgatcgtg atcaggccgg ccacgccacc gtattcgtcg acgacgatgg
     2101 ccatgtgatt gtggttggcg cggaaatcgc gcagcagcac gttgaggcgt ttcgattcag
     2161 ggatgaacac agccgggcgc agcatgtcgc gcacgtcgaa ggatgcttcg gtgtaataac
     2221 gcagcaaatc cttggccagc agcatgccga tcaccttgtc gcgttcgcct tcgactgctg
     2281 gaaagcgcga gtgcgctgtt gatagcacat caggcagcca gctttctatg gggttgctga
     2341 tgtcgatggt atccatttgc gcgcgcggca ccatgatgtc gcgcacggcc agatcggata
     2401 cctgaaacac gccttcgatc atcgacagcg cgtcggcatc gatcagattg cgcgcgtgcg
     2461 catcttgcag gacttcgagc aactctgctc ggttttcagg ttcaggggag atgaaggcag
     2521 taagacgttc aaataaggag cggtgtggtt tagcgtcaga agatttgacg ctactggggt
     2581 gatcttgcat atggcgtaag ccgtgggtcc gacgttgttt cgatgagcta taggatacaa
     2641 caaatcagca gcggtggact attgttgcag caccagcgaa cccggcaggc tgtgcagata
     2701 agcggcgata tcctgcatat cctggtccgt cagcatctcg acctggccgc tcatgatggc
     2761 gttcacgcgc ccgttcatgc cgtcgttgcc gcgccgatag gccatcagcg tgtgttgcag
     2821 gtagtcctga tgctggcctg ccagtttcgg ataggacgga tcgatcgatg agctgaaatc
     2881 cttgccatgg caggaggcgc agttgtattt ttcaaccagc agtttgccgg ttttggcatc
     2941 accagcggcg actgcgttca gggacatcag gaaaaaaatg ggaaaaatca tccatctcag
     3001 tacctttttc atctcgggct ttccttagag cgtgtcatgc agctcttatt tttgctgtgc
     3061 ataataagca gccagggcag cgatgtcctg gtcggacagg ctggcggcga tgccctgcat
     3121 ggacgtatgc ctgcgctcgc ccttttgata ggctttcagc gcattttcga tatatttttc
     3181 agattgaccg cccagcatgg ggacttgata gacttcgggg aaagtcgctt tatagcctgg
     3241 aatgccgtgg cagccgatgc acatcgacac attgactgtg ccggtattac cggcgagatc
     3301 aacggcggaa accgcaccgg cggcacctgt caaagcgagc agtatcaaca gttttttcat
     3361 catcctgacc aaagggtttc atttcctgga tctctgacag tccctgcaa
//
LOCUS       DASRDH010000214         5209 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_421113,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000214 DASRDH010000000
VERSION     DASRDH010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 5209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5209
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421113"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="VFA14_04910"
     CDS             <1..200
                     /locus_tag="VFA14_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HZS80749.1"
                     /translation="SGNAWMHAKLLLVVLAIGYHHACSALLRKFECGQNQRSHQWLRW
                     FNEIPALLLLAIVVLVVVKPF"
     gene            203..1444
                     /locus_tag="VFA14_04915"
     CDS             203..1444
                     /locus_tag="VFA14_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HZS80750.1"
                     /translation="MRARYSYFCPCPRGLEAALAEELGEIAQLAGPDAGLEVHNQVPG
                     GVHCSGSIISAYQLNLRSRIASRVLLRIAHGAYKNENDIYDLTLEQKWESWFKVSRTI
                     RVDVTAVKSSLRSLEFVTLKIKDAICDRFRERFDERPSVNTRVPDMRIAGFLDAHNFT
                     IYLDTSGEALFKRGWRGETGAAPLRENLAAGLLRSSGWKPGMALFDPMCGSGTILVEA
                     AQMLAGIPPGAGRHFAFEKFDNFDTSVWLTIKGDVKPNPLPTVPTIFGSDISGDMVAI
                     TRRNLRTAGIQFEVPLKQIEAQEVKPPVDADGKPVTGIMLTNPPYGERISVRGDSALP
                     TDELLLSFFSAFGTTLKQRFTDWSVFLFSADPGLPKLLRLKEARKTPFFNGALECRLF
                     RFDMVAGFHRREAAKPSSDQT"
     gene            complement(1542..2021)
                     /locus_tag="VFA14_04920"
     CDS             complement(1542..2021)
                     /locus_tag="VFA14_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS80751.1"
                     /translation="MLNMHKCIHGLVILSLSILYISQAFALGGTTYFSGSIVEPACFV
                     NTQHIAPTIPATPRPTYRISLNVHCNAGQSVQISVQSNGTTIDSNMLSNSARENIVIS
                     HHTSLTSYGSTINYQLGRKKDSRKKETVIPLTATLHKAGNTNTTQMSGNVLVSFNYH"
     gene            2379..3047
                     /locus_tag="VFA14_04925"
     CDS             2379..3047
                     /locus_tag="VFA14_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007879971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZS80752.1"
                     /translation="MSLTATKVVRVAIADDHPVVLNALKHELGRLPAFRIVLIAHTGE
                     ALLELLECTPCEVVITDFAMPTNDKDEADGFVLIKRIRTNFPAAKLIVYTAMRNNAVV
                     KRLYRMGVFSVISKKEKANELVNACLETQLDKQVYFPVSLRGELEMSWAQGDAFTQNK
                     ELTMSELEVMRLFVGGSSLSDICERLSRTPSTVSTHKNNAMRKLGLTTDADLIKYAYA
                     TGMI"
     gene            complement(3075..3317)
                     /locus_tag="VFA14_04930"
     CDS             complement(3075..3317)
                     /locus_tag="VFA14_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS80753.1"
                     /translation="MMQTTENTSRPRFDCASDVSTQTTHAASLQQSYLRSHQPHVLTD
                     ALKAQLAVDNQQRRRRLYPRLEQTLAIIEQNSITRS"
     gene            3599..4570
                     /locus_tag="VFA14_04935"
     CDS             3599..4570
                     /locus_tag="VFA14_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HZS80754.1"
                     /translation="MKLKNRIAISVLSLVSGLVFASDQPIIGLITKTEINPYFVKMKE
                     GAQEAAKAKGVRLLTASGKFDGDNASQVAAIENMVAVGAKTILITPNDSKAIVPAIKK
                     AREQGVMVIALDTPTNPREAADALFATDNVKAGILIGQYAKAAFGDRPMKIATLNLSP
                     GKLSGIQRHDGFLQGLGIKEGDASVVCAQDSYGDQAKGQTAMENCLQKNPDINLVYAI
                     NEPAAAGAYKALKAAGKEKNVMLVSIDGACEGVRNIKAGFITATSQQYPLKMAVLGVE
                     AGSDYARTGKKVSGYVDTGVTLITDKKQKDIDSKDSNFGLDSCWGRK"
     gene            4736..>5209
                     /locus_tag="VFA14_04940"
     CDS             4736..>5209
                     /locus_tag="VFA14_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZS80755.1"
                     /translation="MRAILSSLGPCLALLAACIFFATQSDRFLSGHNFSLILQQVMVI
                     GVIAIGQTLIILTAGIDLSCGMVMALGSIVMTKLASDHGMNPYFAVACSMLACTLIGY
                     VNGFLVTKIRLPSFIVTLGTMNIVFAVTQLYSGAQTVTDLPDALLFLGSTFQIGQT"
BASE COUNT         1268 a         1309 c         1342 g         1290 t
ORIGIN      
        1 gatccggtaa cgcctggatg catgccaaac tgttactggt ggtgctggcg atcggctacc
       61 atcacgcctg cagcgcgcta ctcaggaaat ttgagtgtgg tcaaaaccag cgtagccatc
      121 agtggttgcg ctggtttaat gaaatccctg cattgctgct gctggcgatt gtagttctgg
      181 tcgtcgtcaa acctttttga tcatgcgcgc cagatattcc tatttttgcc cctgtccgcg
      241 cggtctcgaa gccgcgctcg ctgaagaact tggtgaaatc gctcaactgg ccggtcccga
      301 cgccggcctc gaggtccaca atcaggttcc tggcggtgtg cattgttccg gcagcatcat
      361 cagcgcctat cagctcaacc tgcgttcgcg catcgccagt cgcgtgctgc tgcgcatcgc
      421 ccatggtgct tataaaaacg aaaatgacat ctacgacctc acgctcgagc agaagtggga
      481 aagctggttc aaggtgtcgc gcacgatccg cgtcgatgtc accgcagtca aatcgtcact
      541 gcgcagtctt gaatttgtca cactgaaaat caaggacgcc atctgtgacc gtttccgcga
      601 acgcttcgac gaacggccat cggtcaacac cagagtgccg gacatgcgta tcgccggctt
      661 cctcgatgca cacaatttca ctatctatct cgacacttcc ggcgaagcgc tgttcaagcg
      721 cggctggcgc ggcgagaccg gcgcagcgcc gctgcgcgaa aaccttgctg ccggcctgct
      781 gcgcagcagc ggctggaagc caggcatggc gctgttcgac ccgatgtgcg gctccggcac
      841 tatccttgtg gaagcggcgc aaatgctggc tggcattcct cctggtgcag gccgccattt
      901 tgcgtttgaa aaattcgaca atttcgacac cagcgtctgg ctgacaatta agggtgatgt
      961 caagccgaat ccgttaccga cggtgccgac cattttcggt agtgacattt ccggcgacat
     1021 ggtcgctatc acacgccgta acctacgcac agccggcatc cagttcgagg taccgttgaa
     1081 gcagatcgaa gcgcaggaag tcaaaccgcc agtggatgcc gatggtaaac cggtcaccgg
     1141 catcatgcta accaacccgc cttacggtga gcgcatcagc gtgcgtggcg acagcgcatt
     1201 gccgaccgat gagctgttgc tgtccttttt cagcgccttc ggcaccacac tcaaacaacg
     1261 ctttaccgac tggagcgtct tcctgttttc ggcagatccc ggcctgccga aactattacg
     1321 tctgaaagaa gcacgcaaga cgccgttctt caacggcgcg ctggagtgtc gcctgtttcg
     1381 cttcgacatg gtggcgggct tccaccgtcg cgaggctgcc aaacccagct ccgatcagac
     1441 ttgaatcctt gtctgacatt ctgctgtgaa gcagtgattc agccaacgta tgatgacgca
     1501 tgtactggta caggtagtta tgccagcatg catcgcagca ttcagtggta gttgaatgac
     1561 accagaacgt taccgctcat ctgtgtcgta ttggtgttac ctgctttgtg cagggtagct
     1621 gtcaacggga tcaccgtctc cttttttcta ctatcctttt tcctaccgag ctgataattg
     1681 attgtgctgc cgtagctagt caatgacgta tggtgactga tcacaatatt ttctctggcg
     1741 ctattgctga gcatgttgct atcaatagta gtaccgttgc tttgtaccga aatctgcacg
     1801 gattgtcccg cattacaatg gacattcaga gagattcgat acgtaggtct aggcgttgct
     1861 ggtatagttg gagcaatgtg ttgagtgttg acgaaacatg ctggttcgac gatcgaaccg
     1921 gaaaaatatg ttgttccacc tagggcaaat gcctgggaaa tataaagaat actcagggat
     1981 aaaataacca aaccatggat gcatttatgc atatttaaca tggtgatact ccttgaaata
     2041 accaataaag aaacagaatt ccgcgatcac atataataaa aatttaatat aaatatcgaa
     2101 taatttcgat gattatttaa tatttttcta tcttatatat agaaataata ttgtattttg
     2161 ttgctttacg ccaattcttt tgctgatcaa aaaaaaaaaa aatagtgagc gttcatagga
     2221 gaaaagataa ctggtagttt ttttgaaaag ataggacggg aaccaagcgc aagtatcgtt
     2281 gctccagagt atgtatttgg ggaggggcat atagcacata tacgaagtgc gtatgctcaa
     2341 aagcacgccg agcgtgcaaa tcaataactg gagaatgtat gagcctgact gcgactaaag
     2401 tagtacgcgt tgccatcgcg gacgaccatc cggtggtact gaatgctttg aaacatgaat
     2461 tgggccgtct gccagcattc cgtatcgttc tgatagcgca taccggagaa gcgttgctgg
     2521 agttactcga atgcacgcca tgcgaagtgg tgattaccga tttcgccatg ccgaccaatg
     2581 acaaggatga agcagatggt tttgtgctga tcaaacgtat ccgtaccaac ttccccgcgg
     2641 ccaagctgat cgtctatacg gccatgcgca ataacgcggt cgtcaagcgt ctgtatcgga
     2701 tgggtgtatt ttcggtgatt agcaaaaagg aaaaggccaa tgaactggtc aatgcttgcc
     2761 tggagacaca actggacaag caggtttatt tcccggtctc cctgcgcggc gaattggaga
     2821 tgagttgggc gcagggcgat gcttttaccc agaacaagga gttgaccatg agcgaactcg
     2881 aagtaatgcg tttgtttgtt ggcggtagtt cgctcagcga catttgtgaa cgcctgtcgc
     2941 gcacgccaag tacagtaagt actcacaaaa acaatgccat gcgcaaactg ggactgacaa
     3001 cggatgctga tttgatcaaa tatgcctacg ctaccgggat gatttgagct cactcagctt
     3061 ggcgatagca gttgctagct acgcgtgatg ctgttctgtt cgatgatggc caaggtctgc
     3121 tccagtcgtg gataaagacg tctgcgtctc tgctggttgt cgacagccag ctgtgctttg
     3181 agagcatccg tcaacacatg gggctgatga ctgcgcaggt aagactgctg tagcgaggct
     3241 gcgtgcgtcg tctgcgttga tacatcggaa gcgcaatcga atctgggcct gctggtattt
     3301 tctgtagttt gcatcatcct cactcccact cctgtaatga gagaatcttt tgcatgtctt
     3361 ccaatccaat gtacacgaga tatttcaaac gcacgataga tgagtgacaa cgtttacttt
     3421 ttgtttgccg catccagcat agggacgtcc aaaaaatatc aatagcaatg tcatcacatt
     3481 aataataaaa caacttgatt tattaattcg gcgggaatat attgccgaac cggcacagca
     3541 tgttgcccgc gatgacatac actgggttgc tataactaaa aattttaggg gacacaaaat
     3601 gaagttgaaa aaccgcattg ccatatctgt gttgagcctc gtttctggcc ttgtttttgc
     3661 ttctgatcag cccatcattg gcttgatcac caaaacagaa atcaatccat atttcgtaaa
     3721 aatgaaagaa ggggcgcagg aggctgccaa ggccaagggc gtcagattat tgacggcttc
     3781 cggcaaattc gatggcgaca atgccagcca ggtcgccgcg atcgagaaca tggttgcggt
     3841 cggcgcgaaa accattctta tcacgccgaa tgactccaaa gccatcgtcc cagccatcaa
     3901 gaaggcgcgt gagcaaggcg tgatggtgat tgcgctcgat acccccacta atcccaggga
     3961 ggcggctgat gcgctatttg ccaccgacaa tgtcaaggct ggcatcttga tcggtcaata
     4021 tgcgaaagcc gcctttggtg acaggccaat gaagatcgcg accctgaatc tgtctcccgg
     4081 caaattgagc ggtattcagc gccatgacgg tttcctgcaa ggtttaggca tcaaggaggg
     4141 agatgcgagc gtggtctgcg cgcaagatag ttacggcgac caggccaagg gccagaccgc
     4201 catggaaaat tgtctgcaaa aaaacccgga catcaacctt gtttacgcca tcaatgagcc
     4261 tgccgctgcc ggtgcctaca aggcgctcaa agcagccggc aaagaaaaaa acgtgatgct
     4321 cgtctccatc gatggcgctt gtgagggtgt gcgcaacata aaagcaggtt tcatcaccgc
     4381 gacctcgcag cagtatccgt tgaaaatggc tgtacttggc gttgaagcgg ggagcgatta
     4441 tgccaggact ggcaagaagg tgagcggtta tgtcgacacc ggtgtaacat tgattaccga
     4501 taaaaagcaa aaagacatcg acagcaaaga tagcaatttc ggtctggact cctgctgggg
     4561 caggaaatga ggtgaagccg ggtcggcttc gatagctgtc ctagccaaca taccgccggc
     4621 cttttttccg gaccaataag gatattcgtt gatgaaactt cgcctgggac acttgccctc
     4681 actctcttcg ttcactcctc atcttgcact gctggcagcc aaggacactg ggcagatgcg
     4741 cgccatcctg tcttcgctcg gcccctgtct cgcactgctg gcagcctgca ttttttttgc
     4801 aacgcaatca gatcgcttcc tcagtggcca taatttctca ctgatcctgc aacaggtcat
     4861 ggtgattggc gtaattgcaa ttggtcagac cttgatcatt ttgacggccg gtatcgatct
     4921 gtcatgcggc atggtgatgg cgctaggctc catcgtgatg accaaacttg cgagcgatca
     4981 cggtatgaat ccttattttg ccgttgcctg tagcatgctg gcttgcactt tgatcggtta
     5041 cgtcaacggt ttcctggtga caaaaatcag gctgccctcg ttcatcgtga ctttgggtac
     5101 catgaatatt gtattcgccg tcacccagct ttattccggc gcgcaaaccg tcaccgattt
     5161 accggacgcg ctgctgtttt tgggcagtac ctttcagatc ggtcagacc
//
LOCUS       DASRDH010000215         7423 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_252117,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000215 DASRDH010000000
VERSION     DASRDH010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 7423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7423
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252117"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1205
                     /locus_tag="VFA14_04945"
     CDS             <1..1205
                     /locus_tag="VFA14_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020700725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HZS80756.1"
                     /translation="FIPASGSAYSYTYVSIGEFPAYIVGFCLLLEYALAASATAIGWS
                     EYLNSFLQHMVDWSIPSHWRSPLVVNGVHGMEMHWGHFNLPPVILVCLCCFLLMRGAR
                     ESATVNGVMVFIKIAILVFFSAVAFTAFNSRNFIPFNPHGMLGQAGEKNMGIVSAAGT
                     IFFSFIGLDTVATAGAETDNPKRNVPLGILLALLIVVLAYLLVATAAMGAQPVAAFTG
                     QEAGLAAILNQVTGQKWPAILLSAGAIVSVFSVTLVLIYGQTRILFVIGRDGLIPEVF
                     QNVHPRTLSPVANTVIVSLMVGLIAGFVDSGYLWDMVSMGTLVAFALVSIGVTVMRLK
                     SPELVRGFTLPFGPWVIPSLSVISCVWIIVGLSNETYTLFTVWISLAVLGYAMYGMRN
                     SRLALKRA"
     gene            1252..2940
                     /gene="hutU"
                     /locus_tag="VFA14_04950"
     CDS             1252..2940
                     /gene="hutU"
                     /locus_tag="VFA14_04950"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HZS80757.1"
                     /translation="MTTHPDPVRVIRAPRGSRLHCKNWLTEAAWRMLQNNLDPEVAED
                     PKNLVVYGGIGRAARDWACFDQILASLKELNHDESLLVQSGKPVGVFKTHPDAPRVLI
                     ANSNLVPKWADWEHFNALDRKGLFMYGQMTAGSWIYIGSQGIVQGTFETFVEASRQHF
                     NNDLGGKWILTAGLGGMGGAQPLAATLAGAVSLTIECQQSRIDFRLRTRYVDKQARDI
                     DDAIALVKQHTAARDAVSIALLGNAAEILPELVRRAKAGGMKPDLVTDQTSAHDLING
                     YLPAGWSVTQWKAAQRDPTRHAQLSAAAAKSCALHVQAMLDFQAMGIPTVDYGNNIRQ
                     VAFDMGVENAFDFPGFVPAYIRPLFCEGKGPFRWVALSGDPEDIYKTDAKVKELFPEN
                     RHTHRWLDMARERIAFQGLPARICWLGLGERHRAGLAFNEMVRTGELKAPIVIGRDHL
                     DTGSVASPNRETEAMKDGTDAVSDWPLLNALLNTASGATWVSLHHGGGVGMGYSQHAG
                     MVIVADGTAAAAGRLARVLVNDCASGVMRHADAGYELAIATARKQGLNLPMISA"
     gene            2962..4527
                     /gene="hutH"
                     /locus_tag="VFA14_04955"
     CDS             2962..4527
                     /gene="hutH"
                     /locus_tag="VFA14_04955"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HZS80758.1"
                     /translation="MKTPLTLQPGNISLAALRSIWADGVPLRLAASASDAIDASAATV
                     ASIVARGDAAYGINTGFGKLARTRIPDAQLELLQRNLILSHAVGTGASMADETVRLIL
                     AMKIGSLARGFSGIRAAVIDTLLAIYNAGIMPCIPAKGSVGASGDLAPLAHMTLALMG
                     EGQVRVDGELRDAGAALAKAGIAPIRLAAKEGLALINGTQASTALALHGLFLAERVLE
                     AATVTGALAVDAARGSDAPFDPRVHEVRGQPGQIAIAKIYHHLLSGSAIRQSHLVNDE
                     RVQDPYSLRCQPQVMGACVDLIGNAARTLLIEANAVTDNPLIYHERDEVISGGNFHAE
                     PVAFAADTLALAIAEIGALSERRIALLIDASISGLPPFLVHEPGLNSGFMIAHVTAAA
                     LASENKSMAHPASVDSLPTSANQEDHVSMASYAGRRLHDMAHNTATIIAIEWLAAAQG
                     VNFHQPLTTSPLLAQAQRRLRDEVGFYDRDRFFAPDIEAARQLVLQGAVSAGCSALFA
                     DLYSGCRHAVSQY"
     gene            4508..5755
                     /gene="hutI"
                     /locus_tag="VFA14_04960"
     CDS             4508..5755
                     /gene="hutI"
                     /locus_tag="VFA14_04960"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HZS80759.1"
                     /translation="MPYRNIKSTQSTDGIWHHARIAPGGNPEQTIRDAAIVVRNGVIA
                     WIGNAAAMPAEFRDLRLPQHDVANRWITPGLIDCHTHLVYGGNRADEFALRLAGASYE
                     EIARRGGGIVSTVRATRATDEDSLFQQAARRLEALLAEGVTTIEIKSGYGLDLPTERK
                     MLRVARRLGASYPVTVYTTFLGAHALPPEYQDRADAYIDLVCNEMLPSLHAEGLIDAV
                     DVFCENIGFSVAQSARVCTAADKLGLPLKMHADQLSNMGATQLAARFKALSVDHLEHL
                     SEADVIAMHKSGTVAVLLPGAYYFIRATKQPPLTLLRRYRIPMAIATDSNPGSSPTTS
                     LLLMLNMSCTLFRMTVAEALLGVTANAALALGKADRHGLLAVGRAADFAIWSIDSLAE
                     LAYWSGYNLCSAVVRGGLLSQPS"
     gene            5769..7148
                     /locus_tag="VFA14_04965"
     CDS             5769..7148
                     /locus_tag="VFA14_04965"
                     /EC_number="3.5.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamate deiminase"
                     /protein_id="HZS80760.1"
                     /translation="MPGCIADCALLPEGWRNDVLLEWDGRGMLTRVSPDAGQATQMPR
                     VAGPVLPGIVNLHSHAFQRALAGLTEYRSDPRDSFWSWRSLMYQFAGKLSPDDLKAIA
                     VQSYIEMLQAGYTSVCEFHYLHHDRDGKPYANPAENMVRLIEAAQETGIGLTLLPVMY
                     QYSGFGAQAPHTGQKRFLNSPQWIMQTLQTLQASHPQHAGLRYGVAPHSLRAVTPQSL
                     QTLLTQFRQWDAQAPVHMHIAEQNQEVQDCLAALGARPVAWLLDNMAVDQHWCLVHAT
                     HMSTTETEKLAQCGAIAGICPTTEANLGDGIFNGVAYAAAQGVWGIGSDSHISASVSE
                     ELRWYEYSQRLRHGQRNMLTGGNAASVGAYLYRQAVHGGAAASGRQVNGLQCGQRADL
                     LVLDPQHPDLNGRHGDQLLDSLVFCRHGQTPLRNVMVGGHWLIKDRRHARQEQSALSY
                     MRVMKTLLE"
     gene            7188..>7423
                     /locus_tag="VFA14_04970"
     CDS             7188..>7423
                     /locus_tag="VFA14_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HZS80761.1"
                     /translation="MHLPIEKFLFHRGRLPLLVSMPHTGEYIPPDIRIDMSAAALQMA
                     DTDWHLPRLYNFLEGLGASILVATHSRYVIDLNRP"
BASE COUNT         1395 a         2232 c         2290 g         1506 t
ORIGIN      
        1 ctttcatccc cgcatccggt tccgcctatt cttataccta tgtcagcatc ggcgaatttc
       61 ccgcctacat cgtcggtttc tgcctgctgc tcgaatatgc gctggccgcc agcgcgacag
      121 ccatcggctg gtctgaatat ctgaacagtt ttctgcaaca catggtcgac tggtcgatcc
      181 catcgcactg gcgcagtccc ctggtcgtca atggtgtgca tggcatggaa atgcattggg
      241 gtcatttcaa tctgccgccc gtcatcctgg tgtgtctgtg ctgttttttg ctgatgcgtg
      301 gcgccaggga gtcggcgacg gtcaatggcg tcatggtatt catcaagatt gccattctgg
      361 tgtttttttc tgcggtggcg tttactgcat tcaatagccg caatttcatt cctttcaacc
      421 cgcacggtat gctggggcaa gctggcgaga agaacatggg gatcgtctcc gccgcaggca
      481 ccatcttttt ttccttcatc ggactcgata cagtcgccac cgccggcgcg gaaaccgaca
      541 acccgaaacg caacgtgccc ttgggcatcc tgctggcgtt gctgattgtc gtgctagctt
      601 atcttctggt ggctacggcc gcgatgggcg cacagccggt ggcggcgttt accggacagg
      661 aggccggtct tgccgcgatt ctgaatcagg tgaccggaca gaaatggcct gccatcctgt
      721 tgtctgccgg cgccatcgtg tcggtattca gcgtcactct ggtattgatt tatggacaaa
      781 cccgcatcct gttcgtgatc ggtcgtgatg gcctgattcc tgaggtgttc cagaacgtcc
      841 acccgcgcac tctctcgcca gtggccaata ccgtgatcgt gtcgctcatg gtgggactga
      901 ttgccgggtt cgtggactct ggctatctgt gggatatggt cagcatgggg accctggtgg
      961 cttttgcttt ggtctctatc ggcgtgacgg tcatgcgcct gaaatctcct gaactggtgc
     1021 gcggcttcac cttgcctttt ggcccctggg tgattccgtc gctgagcgtg atttcctgcg
     1081 tctggatcat cgtgggcttg agcaacgaga cgtataccct gtttactgtg tggatcagcc
     1141 tggcggtgct gggctatgcc atgtacggca tgcgcaactc cagacttgcc ttgaagagag
     1201 cctgaatcag gcaagccagt accctgttta tcgatatgga aaaggaaagt catgacaaca
     1261 catcccgatc ccgtgcgggt gattcgcgcg ccgcgcggta gccgcttgca ttgcaagaac
     1321 tggctgaccg aagccgcctg gcgcatgttg caaaacaacc tcgaccccga ggtcgctgaa
     1381 gatccaaaga atctggtggt gtacggcggg atcggtcgcg ctgcgcgtga ctgggcctgt
     1441 tttgaccaga tactcgcttc attgaaagag ctcaaccacg acgagtcgct gctggtgcag
     1501 tcgggcaaac cagtgggcgt gttcaagacc cacccggacg cgccacgcgt gctgatcgcc
     1561 aattccaatc tggtgccgaa gtgggccgac tgggagcatt tcaatgcgct cgaccgcaag
     1621 ggcctgttca tgtacggcca gatgactgcc ggcagctgga tttacatcgg cagccagggc
     1681 atcgtgcaag gtacgttcga gactttcgtc gaagccagcc gtcagcattt caacaatgat
     1741 ctgggcggca agtggattct gaccgccggt ctgggcggca tgggcggcgc gcaaccgctg
     1801 gcggcgacgc tggccggcgc ggtttcgctc actatcgaat gtcagcaaag ccgcatcgac
     1861 ttccggctgc gtacccgtta tgtcgacaag caggccaggg atatcgatga cgccatcgct
     1921 ctggtcaagc agcacaccgc tgccagggac gcggtgtcga tcgccttgct gggcaatgcc
     1981 gccgagatcc tgccggagct ggtgcgtcgc gccaaggcag gcggcatgaa gccggatctg
     2041 gtgaccgacc aaacttccgc gcatgacttg atcaacggct atctgccggc cggctggagt
     2101 gtgacgcagt ggaaagccgc gcagcgcgac ccgacccggc acgcacaact gagcgccgcc
     2161 gccgcgaaaa gctgtgcgct acacgtgcaa gccatgctgg attttcaggc catgggcatt
     2221 ccgacggtcg attacggtaa caacatccgt caggtcgcgt ttgacatggg tgtcgagaat
     2281 gccttcgatt tccccggttt tgtgccggcg tatatccggc cactgttttg cgaaggcaag
     2341 gggccatttc gctgggtggc gctgtcgggc gatccggaag acatctataa gaccgatgcc
     2401 aaggtcaagg agctgttccc ggaaaaccgg catacgcatc gctggctcga catggcgcgc
     2461 gagcgcatcg ctttccaggg tttgccggca cgtatctgct ggcttggcct gggcgaacgc
     2521 caccgtgccg gtctggcgtt caacgaaatg gtcaggacag gcgagttgaa agcgccgatc
     2581 gtgatcggcc gcgaccatct cgacaccggc tcggtcgcca gccccaaccg cgagacggaa
     2641 gcgatgaaag acggcaccga cgccgtcagc gactggccgt tgctgaacgc cttgctgaat
     2701 accgccagcg gcgcgacctg ggtcagcctg catcacggcg gcggtgtcgg catgggttat
     2761 tcgcagcatg ctggcatggt gatcgtggcc gacggcactg ccgcggcggc cgggcgtctg
     2821 gcgcgcgtgc tggtcaatga ttgcgcctcg ggcgtgatgc gtcatgccga tgccggttac
     2881 gaactcgcca tcgccaccgc gagaaaacag ggcttgaatc tgccaatgat ctctgcttga
     2941 accgcgtcgg aagaaatcat catgaaaaca ccattgacgc tgcaacccgg caacatctct
     3001 ctggcagcgc tgcgcagtat ctgggccgac ggcgtgccgc tgcgactggc agcgtccgcc
     3061 agtgatgcca tcgatgcctc ggccgcgacg gtcgccagca tcgtggcgcg cggcgatgct
     3121 gcctatggca tcaataccgg cttcggcaaa ctggctagaa cccggattcc ggatgcgcaa
     3181 ctggaactgc tgcaacgcaa tctgattctg tcgcacgcgg tcggcactgg cgcatcgatg
     3241 gccgatgaaa cggtacgcct gatcctggcg atgaaaatcg gcagccttgc gcgcggcttt
     3301 tccggcatcc gggccgcggt catcgatacc ttgctggcga tctacaacgc cggcatcatg
     3361 ccctgcattc cggccaaggg atcggtcggg gcttccggcg atctggcgcc actggcgcac
     3421 atgacgctgg cgctcatggg cgagggccag gttcgggtcg acggcgaact cagggatgcc
     3481 ggagcagcac tggccaaggc cggcatcgcg ccgatcaggc tggccgccaa ggaaggactg
     3541 gcgctgatca atggcaccca ggcttctacc gcgctggcgc tgcatggcct gttcctggcg
     3601 gagcgcgtgc tggaagccgc gaccgtgact ggcgcgctgg cggtggatgc ggcgcgcggc
     3661 agcgatgcgc cgttcgaccc gcgcgtgcat gaagtgcgtg gccagccggg acagattgcc
     3721 attgcgaaga tttatcatca cctattgagc ggcagcgcga tccggcaatc gcatctggtc
     3781 aatgatgagc gagtacagga tccctatagc ctgcgttgcc agccgcaggt gatgggcgcc
     3841 tgtgtcgatc tgatcggcaa tgcggcgcgc acgctgctga tcgaggcgaa tgccgtgacc
     3901 gacaacccct tgatttatca cgagcgcgac gaagtgattt ccggcggtaa tttccatgct
     3961 gaaccggtcg cgttcgcggc ggatacgctg gcgctggcca tcgccgaaat cggcgccctg
     4021 tcggaacgcc gcatcgcttt gctgatcgat gcatcgattt ccggtctgcc gccattcctg
     4081 gtgcatgaac cgggtctgaa ttccggtttc atgattgccc acgtgacagc cgccgcgctg
     4141 gcctcagaaa acaaatccat ggcgcatccg gccagcgtcg atagtttacc gacttccgcc
     4201 aatcaggaag accacgtcag catggcaagc tacgcggggc gccgtctgca tgacatggcg
     4261 cacaacactg caaccatcat cgcgattgaa tggctggcgg cggcgcaggg cgtgaatttc
     4321 catcagccgc tgacgacttc accattgctg gcgcaagcac agcggcgctt gcgtgacgag
     4381 gtcgggttct acgacaggga ccgcttcttc gcgccggaca tcgaagccgc ccggcaactg
     4441 gtcctgcaag gcgcggtgag cgccggttgc agtgccttgt ttgcggatct gtactcggga
     4501 tgccgacatg ccgtatcgca atattaagtc aacgcagtcc accgacggca tctggcatca
     4561 cgccagaatc gctccgggcg gcaatcctga gcagaccatc cgcgacgccg ccatcgtggt
     4621 acgcaatggc gtcattgcct ggatcggcaa cgcggcggcg atgcccgcgg aattccgtga
     4681 tctccgcctg ccgcagcacg atgtcgccaa ccgctggatc acgcccggtc tgatcgattg
     4741 ccatacccat ctggtgtatg gcggcaatcg cgccgatgaa ttcgccttac gtctggccgg
     4801 cgccagttat gaagaaatcg cccgtcgcgg cggcggcatc gtctccaccg tgcgcgccac
     4861 ccgcgcgacc gacgaagaca gcttgtttca gcaggcggca agaagactgg aagccctgct
     4921 ggcggaaggc gtgaccacga tagaaatcaa atccggctat ggcctggatc tgccgaccga
     4981 gcgtaaaatg ctgcgcgtcg cgcgccgtct cggcgcatcg tatccggtca cggtgtatac
     5041 cacgtttctg ggcgcgcatg cgctgccgcc ggaatatcag gatcgtgccg acgcctacat
     5101 cgatctggtc tgcaatgaga tgctgccatc gctgcatgcc gagggtttga tcgatgcggt
     5161 cgatgtgttt tgcgaaaaca tcggtttttc cgtggcgcaa agtgccaggg tctgcacagc
     5221 agccgacaag ctcggcctgc cgctgaaaat gcatgccgat caattgtcga atatgggcgc
     5281 gacgcaactg gccgcgcgtt tcaaagcgtt gtcggtcgat cacctggagc atctgagcga
     5341 agccgacgtg atcgccatgc acaagagcgg cacggtggcg gtattgctac ccggcgccta
     5401 ctacttcatc cgcgcaacca aacagccgcc gctgacattg ctgcggcgct accggatacc
     5461 gatggcgatc gccaccgaca gcaatccggg cagctcaccc acgacctccc tgctgctgat
     5521 gctgaacatg tcctgcaccc tgttccgcat gacagtggcg gaagccttgc tgggcgtgac
     5581 cgccaatgcc gccctggcgc tcggcaaagc ggatcgtcac ggtttgctgg cagtgggtcg
     5641 ggctgccgat tttgcgatct ggtccatcga ttcgctggct gaactggcat actggtccgg
     5701 ctacaatctg tgcagcgcag tcgtgcgcgg cggtctgctc agtcaaccat cgtaaaggga
     5761 atattgtcat gccaggatgc attgccgact gcgccctgct gcccgaaggc tggcgcaatg
     5821 atgtcctgct ggaatgggat gggcgcggca tgctgacccg tgtcagtcct gacgccgggc
     5881 aagctacgca gatgccgcgc gtcgctggtc cggtgctgcc gggcatcgtc aatctgcatt
     5941 cgcatgcatt tcagagggcg ctggcgggac tgacggaata tcgttccgat ccacgcgaca
     6001 gtttctggag ctggcgcagc ctgatgtacc agtttgccgg caagctgtcg ccggatgatc
     6061 tgaaggcgat tgccgtgcag tcgtacatcg agatgctgca agccggctat acctcggtct
     6121 gcgaattcca ttacctgcat cacgatcgcg acggcaagcc gtatgccaat ccggcagaaa
     6181 acatggtacg cctgatcgaa gctgcacagg aaaccggcat cggcctgacc ttgctgccgg
     6241 tcatgtatca atacagcggc tttggcgcac aggcgccgca tacgggacag aaacgttttc
     6301 tcaattcgcc acaatggatc atgcagacct tgcagacctt gcaggcatcg catccgcaac
     6361 acgccggcct gcgctacggc gtcgctcccc attccttgcg tgcggtgacg ccgcagtcgt
     6421 tgcaaacgct gttgacgcag ttccgccaat gggatgcgca agcgccggta catatgcata
     6481 tcgccgaaca gaaccaggag gtgcaggact gcctggcggc attgggtgcg cggccagttg
     6541 cctggctgct ggacaatatg gcggtggatc agcactggtg tctggtgcat gccacgcaca
     6601 tgagtacgac agagacagaa aaactggcgc agtgcggcgc catcgccggc atctgcccga
     6661 ccaccgaagc caatctgggc gacggcattt tcaacggcgt tgcctacgcc gcagcgcaag
     6721 gtgtgtgggg catcggttcc gacagccata tcagcgccag cgtcagcgaa gaattgcgct
     6781 ggtacgaata cagtcagcgt ctgcggcatg gtcagcgcaa catgctgacc ggcggcaatg
     6841 ccgcttcggt gggcgcttat ctgtatcgtc aggcggtgca tggcggcgct gccgccagcg
     6901 gccgtcaggt caacggcctg caatgcggac aacgcgccga tctgctggtg ctggacccgc
     6961 aacacccgga tctgaacggc aggcatggcg atcagttact cgacagcctg gtcttttgcc
     7021 ggcacggaca gacgccgctg cgtaatgtca tggtcggcgg tcattggctc atcaaagacc
     7081 gccggcatgc gcgtcaggag cagagcgcct tatcgtatat gcgcgtcatg aagacattac
     7141 tggagtaggt tgttagcggc gctaaagatg gaacgaaagg atgccccatg catttgccca
     7201 tcgagaaatt cctattccat cgtggtcgcc tgccgctgct ggtgtcgatg ccgcatacgg
     7261 gagagtatat tccgccggat atccgcattg acatgagcgc cgcggcgctg cagatggccg
     7321 ataccgactg gcacctgccg cgcctgtaca actttctgga aggactcggc gcatcgattc
     7381 tggtggcaac ccactcacgt tacgtaatcg atctgaaccg ccc
//
LOCUS       DASRDH010000216         8148 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_930488,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000216 DASRDH010000000
VERSION     DASRDH010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 8148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8148
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_930488"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            153..704
                     /locus_tag="VFA14_04975"
     CDS             153..704
                     /locus_tag="VFA14_04975"
                     /inference="COORDINATES: protein motif:HMM:NF023444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HZS80762.1"
                     /translation="MRRCHTGFTLLELLIVFSIVAILLAAGVPSFRTYLHRQQVVTAA
                     NQFLFALNLARSEAIRRASRVDVAPRDGRYWENGWVVFVNHDHRPQFDPGDELLYSYD
                     GLAHELQIATTLSDKSYPYIAYNDSGRTRANLSAQTPQWGSWRFSLHGQERIVRVTLA
                     SRARLCNPADDMSCRFVDAQDSP"
     gene            734..1849
                     /gene="ribD"
                     /locus_tag="VFA14_04980"
     CDS             734..1849
                     /gene="ribD"
                     /locus_tag="VFA14_04980"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016836643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HZS80763.1"
                     /translation="MSLYSIEIDNDRQAMTLALQQAERGMFITMPNPRVGCIIVKDRR
                     IIGAGFTQPPGGNHAEIEAMRDARERGSDMRDATVYVTLEPCCHFGRTPPCADALIEA
                     GISRVVAALPDPNPLVSGKGLEKLQAAGIAVVTGILQEQAHEMNIGFFKRMQQGTPWI
                     RMKSAASLDGKTALHNGKSQWITGQAARDDGHFWRARACAIITGIGTIKDDDPRLNVR
                     AIDTPRQPRRIVIDSKLTLSPSATILAGGGTWVFTASEDRTKITALADRGADIIVLPN
                     ADDKVDLPCVMQELGKRQLNEVHIEAGAKLNASLIRARCVDELLLYLAPSLLGEGRNM
                     FDLPALDDLSGRMMLSFHEIRQIGEDLRILARFNSIT"
     gene            1858..2478
                     /locus_tag="VFA14_04985"
     CDS             1858..2478
                     /locus_tag="VFA14_04985"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006464276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HZS80764.1"
                     /translation="MFTGIVAAVGNITSVQALGLGADAGIRLDIDAGALPMDDVALGD
                     SIALNGACMTVVGKTAQGFTVDVSRESLSLTAGLDAPGEVNLEKALTLAERLGGHLVS
                     GHVDGLGVVRKFAPVGESCELVVEAPKSLGKYLACKGSIVVNGVSLTVNRVEDLIDAC
                     CFSVNLIPHTVQVTTLKHIKVGSKVNLEIDLIARYVERMLSLSQRV"
     gene            2580..3401
                     /gene="mutM"
                     /locus_tag="VFA14_04990"
     CDS             2580..3401
                     /gene="mutM"
                     /locus_tag="VFA14_04990"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HZS80765.1"
                     /translation="MPELPEVEITRRGIAPYLEGNAVTAVVMRHSGLRWPFPADLQQR
                     LAGRQIRSVKRRGKYLLLHFDHGILIIHLGMSGHLRILPLGIAPQKHDHFDLVVGKQV
                     MRMTDPRRFGAVLWHDASAGNIEEHILLRGLGLEPLENRFSAQDLYHRTRNRSMPIKQ
                     VLLAGSIVVGVGNIYASESLFKAGIDPKRAAGKISLPRYEKLVVAIRETLAAAIEQGG
                     SSLRDFLAVNGQSGYFQQNYFVYGRTSQACRVCATPVRQIRQGQRSTFYCVTCQR"
     gene            3398..4498
                     /gene="mutY"
                     /locus_tag="VFA14_04995"
     CDS             3398..4498
                     /gene="mutY"
                     /locus_tag="VFA14_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HZS80766.1"
                     /translation="MKCVINKTDATDDFADPTFSVAVIDWQKQHGRHALPWQNTRDAY
                     RIWLSEIMLQQTQVTAVIPYYQKFLQRFPTVESLAAAASEAVMSYWSGLGYYTRARNL
                     HRCAQRVVQEYGGHFPDDPALLADLPGIGRSTAAAIAAFAYGRHAAILDGNVKRVFAR
                     VFGVEGFPGTKQVEDMLWRRAVALLPLADMAAYTQGLMDLGAILCVRGKPACDRCPLA
                     SRCVAFATNRTAELPARKPKKAIPEKETVMLLISEGEQVLLQQRPDSGIWGGLLSLPE
                     LPVSRNAEEFGLAVARATASFGTIAVCRSLQAFSHVFTHFRLYVSPYQVVLKQRLLQV
                     AESAYVWYPIAELPDAPLPAPVKKLLLGVYKP"
     gene            complement(4495..5358)
                     /gene="rapZ"
                     /locus_tag="VFA14_05000"
     CDS             complement(4495..5358)
                     /gene="rapZ"
                     /locus_tag="VFA14_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HZS80767.1"
                     /translation="MRVVLITGISGSGKSVALRVLEDAGYFCVDNLPPALLRNLVEIR
                     LREGDETLAVATDARSADSLAGLGPDIQFLKDQGHDVKLLFLTATTESLITRFSETRR
                     SHPLSHHLSPNQHPTDRLTLTECIHKEREILSVVEGLGHVIDTSNLSVSRLRPWIRDL
                     IDIDRASLTLLFESFAFKVGVPLDADLVFDARVLPNPHYDVRLRALTGRDAPVVGFLA
                     NLPMVNELLSDIRTFVEKWLPSFKEDSRSYLTVAVGCTGGQHRSVYIVEQLASYFSAN
                     ENVVVRHRQLG"
     gene            5421..6458
                     /gene="argC"
                     /locus_tag="VFA14_05005"
     CDS             5421..6458
                     /gene="argC"
                     /locus_tag="VFA14_05005"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HZS80768.1"
                     /translation="MIKAGIVGGTGYTGVELLRLLVLHPEVQLQAVTSRKEEGMPVAD
                     MYPSLRGRVDITFSAPGNTRLTDCDVVFFATPHGVAMAQAPGLLAAGVKVIDLAADFR
                     LQNTATFEKWYKMPHNCPGLLKEAAYGLVELNRDSIRKARIVGNPGCYPTTMQLGYAP
                     LLKAGVIDALHLIADCKSGVSGAGRKAEIAILFSEASDNFKAYGVSGHRHLPETLEQL
                     SKLTSDKVGLLFTPHLAPMIRGMHSTLYARLSRDIDNATLQALFEDAYKHEPFIDVMP
                     FGAHPETRTTRASNMLRIALHRPDNGDTVVVLVVQDNLLKGAAGQAVQCMNLMFGLEE
                     TAGLLHMPVLP"
     gene            complement(6567..>8148)
                     /locus_tag="VFA14_05010"
     CDS             complement(6567..>8148)
                     /locus_tag="VFA14_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007882451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HZS80769.1"
                     /translation="SLSIDTPKLFFLRDKEARNNWTFKSDSVSKWEVHPGKLVIRKGS
                     VQLDDAIRHIDATAEIATLDPDKEKVYGIGWHIKGSFNETGIHGAGKAGGVLSLQNDH
                     RPYPLEADMRVGKTHIAVKGTLTKPQDLTVLDLRLNLSGDSMAHLFSLTGVLLPETPP
                     FSTAGHLISKLAPGQSDWTYEKFNGKVGSSNISGTLEYQLKQPRPLLKGEIVSSLLQF
                     EDLAPLIGVEERQSSNRMLPTGEFNTGNWNALDAEIKLTAHAIAKKKNLPITDIHAEL
                     QLNDQILSLALLNLGIAGGKLSSTIRLDGRAPEMKSELQLSARHLRIQQLLPGFDRTQ
                     IGFGEINGDAALSAAGNSLAAMLASSNGEFKALVNQGAISQLLLEELGLNPGDVMMSR
                     LFGDRPVNLNCLTSNFTVSNGLMLARTFALDTEDALINITGNINLAQEQLHLTIDPRS
                     KDLRTISLRSPLSVSGPFSHPDISVDKRAALQAGGAVLLGVAAPVTAILPLINVNAKQ
                     KIDCAGLLEEAQKRPTAG"
BASE COUNT         1749 a         2275 c         2303 g         1821 t
ORIGIN      
        1 tgtccgctgt tcccggcgcg gcgcaggtcg atcaggactt tcacgatgaa atatgcggca
       61 cactgacact caacagccga ggcgagagaa ctgcggcgga cctgccgctg gcatcagcgc
      121 caccggtttg ccgttaaacg ggaccgagta ccatgcgcag gtgccacacc ggcttcactc
      181 tgctcgaatt gctgattgta ttcagcatcg tagcgatatt gctggctgct ggagtcccca
      241 gtttccgtac ctatctgcat aggcagcaag tggtgacggc tgccaatcaa tttctgtttg
      301 cgctgaatct ggcacgcagt gaagccatcc gtcgcgccag ccgcgtcgac gtcgccccac
      361 gcgacggccg gtactgggaa aacggctggg tcgtctttgt caaccatgac catcgaccgc
      421 aattcgaccc tggcgatgag ctgctctaca gctatgacgg tctcgctcac gagctgcaga
      481 tcgccaccac gctcagcgac aagtcctacc cttacatcgc ttacaatgac agtggcagga
      541 cacgcgccaa cctgagtgcg cagacgccgc agtggggatc ctggcgattt tcactgcatg
      601 gtcaggaaag aattgtcaga gtgaccctgg ccagccgcgc tcgcctgtgc aacccggcag
      661 acgatatgag ttgcaggttt gtcgacgctc aggacagccc ttgagtgcca ttgcatgtga
      721 caacaaaacc aatatgtccc tctactccat cgagatcgat aacgacaggc aagccatgac
      781 gctggcgttg caacaggccg aacgaggcat gttcatcacc atgcccaatc ctcgcgtcgg
      841 ctgtatcatc gtcaaggatc gccgcatcat cggcgccggc ttcacgcagc cgcccggtgg
      901 caaccatgcc gagatcgagg ccatgcgtga cgcccgcgaa cgcggcagcg acatgcgtga
      961 cgccaccgtc tacgtcactc tggaaccctg ctgccacttc ggccgcaccc ccccctgcgc
     1021 cgatgccctg atcgaagccg gtatcagcag agtggtcgcc gccttgcctg atcccaaccc
     1081 gttggtatcg ggcaagggtc tggaaaaact gcaagccgcc ggcattgccg tcgtgaccgg
     1141 catcctgcaa gaacaggcac atgaaatgaa catcggtttc ttcaagcgca tgcagcaagg
     1201 cacaccctgg atacgtatga aatcagcagc cagcctggat ggaaagaccg ctctgcacaa
     1261 cggcaaaagc caatggatca ccggccaggc agcgcgcgat gacggccatt tctggcgcgc
     1321 ccgcgcctgc gccatcatta ccggcatcgg caccatcaaa gacgacgatc cgcgattgaa
     1381 cgtgcgcgcc atcgatacac cgcgccagcc gcgccgcatc gtcatcgaca gtaagctgac
     1441 gttgtcaccg agcgccacga ttcttgctgg cggcggcacc tgggttttca ctgccagcga
     1501 agatcgtaca aaaatcaccg cactcgcaga tcgaggtgcc gacattatcg tactgcccaa
     1561 tgccgacgac aaagtcgatc tgccgtgcgt gatgcaggaa ctgggaaaac gccagctcaa
     1621 cgaagttcat atcgaagccg gcgccaaact caatgcctcg ctgatccgcg cgcgctgcgt
     1681 cgatgagctg ctgctttatc ttgcgcccag cctgctcggc gaaggacgaa acatgttcga
     1741 cctgccggcg ctggacgact tgtcaggcag aatgatgcta agctttcatg agatcaggca
     1801 aatcggcgaa gatttacgta ttcttgcacg cttcaattca atcacttagg ctccctcatg
     1861 tttacaggta tcgtcgccgc ggtcggcaac atcacctccg ttcaggctct aggccttggc
     1921 gccgatgctg gcatacgcct cgatatcgac gccggcgcac tgccgatgga tgatgtcgcg
     1981 ctgggcgact cgattgccct gaacggcgcc tgcatgacgg tcgtcgggaa aactgcccaa
     2041 ggtttcacgg tcgatgtctc gcgcgaaagc ttgagcctca ctgccggtct cgacgccccc
     2101 ggcgaagtca acctggaaaa agcccttacc ttggccgaac gtctgggtgg ccatctggta
     2161 tcgggtcatg tcgatggtct tggcgtggta cgcaagtttg caccggtcgg tgaatcctgt
     2221 gaactggtcg tcgaagcgcc gaaatcactg ggaaaatact tggcctgcaa aggctccatc
     2281 gtcgtcaacg gcgtctcgct tactgtcaat cgtgtagaag acctcatcga tgcttgctgt
     2341 ttttcggtca atctgattcc gcatactgtt caggtgacga cgttgaaaca catcaaggtc
     2401 ggcagcaagg tcaatctgga gattgatttg attgcgcggt atgtcgagcg aatgctttct
     2461 ttgtctcaaa gagtataaaa atcaataact gtaactccct cgaaaaatag gaccgagatg
     2521 aattagagtt gccgggtttt ttgttgtcgc tctactatgt acttatcgct tgttgcctca
     2581 tgcccgaatt accggaagta gaaatcaccc gccgcggcat tgctccttat ctcgagggca
     2641 acgcggtgac cgccgtggtg atgcgccata gcggattgcg ctggccgttt cctgccgatt
     2701 tgcaacaacg gcttgccggt cgccagattc gttcggtcaa acggcgcggc aagtatctgc
     2761 tactccattt tgaccatggc atcctgatca tacatctggg gatgtcggga cacctgcgta
     2821 ttttgccgtt ggggattgcg ccgcaaaagc acgatcactt cgatctcgtg gtcggcaagc
     2881 aggtcatgcg tatgaccgat ccgcgccgtt ttggcgccgt gctgtggcat gacgcgtccg
     2941 ccggcaatat tgaagaacat atactcttgc gcggtcttgg tctggagccg ctggaaaaca
     3001 gattttccgc gcaggatctg tatcaccgca cgcgcaatcg cagcatgccg atcaagcaag
     3061 tcttgctggc tgggagcatt gtggtcggcg tcggtaatat ttatgcatcg gaaagcctgt
     3121 tcaaggccgg catcgatccc aagagggcag caggaaaaat cagtctgcca cgttacgaaa
     3181 aactggtcgt cgcgatccgc gaaacgctgg ctgccgccat tgaacaaggt ggcagttcct
     3241 tgcgcgattt tcttgccgtt aacggccagt ctggctactt ccagcaaaat tatttcgttt
     3301 atggccgtac cagtcaggca tgccgggtgt gcgcgacgcc ggtccggcaa atcaggcagg
     3361 gtcagcgctc cactttttac tgtgtgactt gccagcgatg aagtgtgtga tcaacaaaac
     3421 cgatgcgacc gatgatttcg ctgacccaac attttctgtt gctgtcatcg actggcaaaa
     3481 gcagcatggt cgccatgccc tgccttggca gaacacgcgc gatgcctatc gcatctggct
     3541 atcggaaatc atgctgcagc agacgcaggt aacggcagtg attccgtatt accagaaatt
     3601 tctccagcgt ttcccgacag tagaaagtct cgccgccgca gcttctgaag cagtcatgtc
     3661 gtactggagc ggactcggtt attacacgcg ggcgcgtaat ctgcatcgct gcgctcagcg
     3721 tgtggtgcag gaatacggtg gacactttcc cgatgatcct gcattgttgg ccgatttgcc
     3781 tgggatcgga cgttcgactg cggcggccat cgctgctttc gcttacggcc ggcatgccgc
     3841 tattctcgat ggcaacgtca aacgggtgtt tgcgcgcgtt ttcggcgtgg aagggtttcc
     3901 cggcacgaag caggtcgagg atatgctgtg gcgtcgcgcc gtcgctttgt taccattggc
     3961 tgatatggcg gcctataccc aaggtctgat ggatctgggt gcaatcttgt gcgtgcgcgg
     4021 caagccggcg tgcgaccgtt gtcctctggc ctcgcgttgc gtggcgttcg ccaccaatcg
     4081 cacggccgaa ctgccggcgc gcaagccgaa gaaggcgata ccggaaaaag aaacggtgat
     4141 gctgctcatc agcgaaggcg agcaggtctt gttgcagcaa cgccctgaca gcggcatctg
     4201 gggcggcctg ttgtccctgc cggagcttcc tgtgagccgc aatgcggaag aatttgggct
     4261 ggcagtcgca agggcgacgg cgtcttttgg aacgatcgcc gtctgtcgca gcttgcaggc
     4321 gttttcacac gtttttacgc atttcagatt atatgtgtcg ccgtatcagg tggtgttgaa
     4381 gcagcgtttg ctgcaagtgg cggaatcggc gtatgtctgg tatccgattg ccgaactgcc
     4441 tgatgcgcct ttgccggcgc cggtcaagaa actgttgctg ggtgtgtaca agccctagcc
     4501 aagctggcga tggcggacca ccacattctc gtttgccgag aaatagctgg ccagttgttc
     4561 gacgatatag actgaacggt gctggccgcc ggtacagcca acggcaaccg tcaggtagct
     4621 gcggctgtct tctttgaagg atggtagcca cttttcgaca aaagtgcgga tgtcgctcag
     4681 tagctcgttt accatcggca agtttgcaag gaatccgacc accggcgcat cgcgtccggt
     4741 cagcgcacgc aagcgcacat cgtagtgcgg gttgggcagg acgcgcgcgt cgaataccag
     4801 atcggcgtcc agcggcacgc cgaccttgaa cgcaaaagat tcgaacagca gggtcagact
     4861 ggcgcgatcg atatcgatca aatcccgaat ccagggacgc agtctgctga cgctcaggtt
     4921 cgaggtatcg atgacatgac caagaccttc gaccacgctc aggatttctc tttccttgtg
     4981 gatacattcg gtcagcgtca gtcggtcggt gggatgctgg ttcggcgaca ggtgatgcga
     5041 cagcggatgg ctgcggcggg tttcagaaaa gcgcgtaatc agcgattccg tggtggccgt
     5101 caggaagagt agcttgacat catgcccctg gtcttttaga aattggatgt ccggccctag
     5161 tccggcaagc gaatcggcgc tacgcgcgtc ggtagcgacc gccaacgttt cgtcgccttc
     5221 tctcagacgg atttcgacca gattgcgtag cagcgctggc ggtaaattgt cgacacaaaa
     5281 atagcctgca tcttccagaa cacgcaaagc cactgatttg ccggaaccgg aaatgccagt
     5341 aatgagaacg acacgcatgg gtatgatgat acctcaacgc cagatgttga taaaatgaaa
     5401 cgctctcagc aaggaaaaaa atgatcaagg caggtattgt cggtggcaca gggtatacgg
     5461 gcgtggagtt gttgcgcctg cttgtattac atcctgaagt acaactgcag gcggtgacat
     5521 cgcgcaagga agaaggtatg ccggttgcag acatgtatcc atcgctgcgc ggccgcgtcg
     5581 atattacttt ttccgcgccc ggcaataccc ggctgaccga ttgtgatgtc gtattctttg
     5641 ccacgccgca tggcgttgcc atggcgcagg ctcctggact tctggcagcc ggtgtcaagg
     5701 tcatcgatct ggcggctgac ttccgcctgc aaaacaccgc caccttcgaa aagtggtaca
     5761 aaatgccgca caattgtccc gggctactca aagaagcagc ttacggtctg gtcgaactca
     5821 atcgcgatag tattcgcaaa gcgcgcattg tcggcaatcc tggctgctat ccgactacca
     5881 tgcaactggg ttatgcgccg ttgctgaaag ccggtgtgat cgatgctctg catctgatcg
     5941 ccgattgcaa atcgggtgtg tcgggcgcag gccgcaaggc agaaatcgcc atactgtttt
     6001 cagaagcttc ggacaacttc aaagcctacg gcgtgtcagg ccatcgacat ttaccggaaa
     6061 cgctggagca actgagcaag ttgaccagcg acaaagtcgg cctgctgttt acgccgcacc
     6121 tggcgccgat gatccgcggc atgcattcga cgctctatgc gcgcttgagc agggatatcg
     6181 acaacgccac tttgcaggcg ttgtttgaag atgcctacaa acacgagcct ttcatcgacg
     6241 tcatgccgtt cggcgcgcat ccggagacgc gcaccacgcg cgcttccaac atgctgcgca
     6301 ttgcactgca tcgtccggat aacggcgata ccgtggtagt actggttgta caggacaacc
     6361 tgctcaaggg cgctgccggt caggctgtac aatgtatgaa tctgatgttc ggactggagg
     6421 aaacggctgg tctgctgcat atgcccgtgt taccgtgaaa tacagaaacg taagctgtct
     6481 cgatgaaaac ggcctgctcc ccgctggtca gggataagca tgcatcaacc cgaggaggcg
     6541 agtccagtca cggagccgag ataagactac ccggcagttg ggcgtttttg cgcttcttcc
     6601 agcagaccgg cgcaatcgat tttttgttta gcgttgacat tgatcaaagg caggattgct
     6661 gttaccggtg ctgcgacacc caacaatacg gcgccacctg cctgcaacgc cgcgcgctta
     6721 tctacactta tatcagggtg gctgaacggt ccactcaccg atagcggtga gcgtaaagag
     6781 atagtgcgca agtctttgct gcgcggatca atggtcagat gaagttgctc ttgagccaga
     6841 ttgatattgc cggtgatatt gatcagagca tcttccgtgt ccagcgcgaa ggtgcgcgcc
     6901 agcatcagac cgttgctgac agtgaagtta ctggtcaaac aattcaggtt gaccggcctg
     6961 tcgccgaaca gtcgggacat catcacatcg ccgggattca aacccagttc ttccaacagc
     7021 agttgactga tggcaccctg attgaccagg gccttgaatt caccattgga ggaagcgagc
     7081 atcgctgcca atgaattgcc ggcggcgctt agcgccgcat caccattgat ttcaccaaag
     7141 ccaatctggg ttctgtcgaa gccgggaagc agttgctgga ttctaaggtg tcgtgccgat
     7201 aattgcagtt ccgacttcat ctccggcgca cggccatcca gcctgatcgt cgaactcagc
     7261 ttgccgccgg cgatcccgag gttcaagagc gccagcgaca aaatctgatc attcaattgc
     7321 agctcggcat ggatatcggt gattggcaga ttttttttct tggcaattgc gtgcgcagtc
     7381 agtttgattt cggcatcgag cgcattccag ttaccggtgt tgaattctcc ggtcggcaac
     7441 atcctgtttg aggactggcg ttcttccaca ccgatcagcg gtgccaggtc ttcaaattgc
     7501 aggagactgg aaacgatttc gcccttcagc aagggccgtg gttgtttcaa ctgatattcg
     7561 agagtgccgc tgatattgct ggagccgacc ttgccgttga atttttcata agtccagtcg
     7621 ctctggccgg gagccagttt gctaatcaga tgaccggcag ttgaaaatgg cggcgtctcc
     7681 ggcagtagca caccagtcag tgaaaacaga tgcgccatgc tgtcgcctga caaattcagc
     7741 cggaggtcca gcactgtcaa gtcctggggt ttggtcagtg tgcctttgac ggcaatgtgg
     7801 gttttgccga cgcgcatatc ggcttccagt gggtaaggtc ggtgatcatt ttgcagcgac
     7861 aacacaccgc cggccttgcc tgctccgtga atgccagttt cgttgaagct gcctttgata
     7921 tgccagccga tgccgtaaac tttctccttg tccggatcaa gtgtagcgat ctcagctgtt
     7981 gcatcgatat gccggatggc atcgtcaagt tggacactac ctttacggat gacgagtttt
     8041 ccaggatgga cctcccattt cgagacgcta tcggatttga aagtccagtt attacgcgcc
     8101 tctttgtcac gcagaaaaaa caacttaggg gtgtcgatgc tcagcgaa
//
LOCUS       DASRDH010000217         2622 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_37249,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000217 DASRDH010000000
VERSION     DASRDH010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37249"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="VFA14_05015"
     CDS             complement(<1..473)
                     /locus_tag="VFA14_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007877433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HZS80770.1"
                     /translation="MTVRFSTAAMTQARQVRLMIFDVDGVLTDGSLYYGPEGEPLKRF
                     NALDGHGIKMLQQSGGVATAIITARQSPMVLRRARDLGIAHVFQDAHDKRLAFAQLLE
                     QTGIAADACGYLGDDVIDLPVLTRVGFKAAVANAHHDVRTRVDYITEACGGDGAAR"
     gene            complement(473..1501)
                     /locus_tag="VFA14_05020"
     CDS             complement(473..1501)
                     /locus_tag="VFA14_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007877431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HZS80771.1"
                     /translation="MSATEYQILPKVFSPESARRALSLARQTLRIEADAILALQARIS
                     DSPDEPLARAVGMLLECKGRVVVSGIGKSGHIGRKIAATFASTGTPALFMHPAEAAHG
                     DLGMVAANDVFIAISNSGETAELMAIVPIIKRMGAHLIAMTGNHDSSLARLSAIHLNI
                     SVSKEACTLNLAPTASTTAMLAMGDALAVSLLDARGFMEEDFARSHPGGALGRRLLTH
                     VRDVMRTGDAVPAVTPDVLLSVALMEITRKGMAMTAVVDGQYRPIGVFTDGDLRRLLE
                     RGEDFTRFSIADVMHHGPRTIMPDQLAVDAVQLMEEFRINQVLVADAQGTLVGALHIH
                     DLTRAKVI"
     gene            complement(1552..2547)
                     /locus_tag="VFA14_05025"
                     /pseudo
     CDS             complement(1552..2547)
                     /locus_tag="VFA14_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008117154.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
BASE COUNT          531 a          779 c          792 g          520 t
ORIGIN      
        1 cgtgcggcgc cgtcgccgcc acaggcttcg gtaatgtaat cgacccgcgt cctgacatca
       61 tggtgagcat tggcgaccgc tgccttgaag ccgactctgg tcagcaccgg cagatcgatc
      121 acgtcgtcgc cgagataacc gcaggcatcg gccgcgatgc cagtctgctc cagcaattgc
      181 gcaaaagcca gacgcttgtc gtgcgcgtcc tgaaagacat gagcgatacc cagatccctg
      241 gcacgacgta acaccatcgg ggattgacgc gcggtgatga ttgccgtcgc cacgccaccg
      301 gattgctgca acattttgat gccatgaccg tcgagcgcat tgaagcgctt gagcggttcg
      361 ccttccgggc cgtaatacag gctaccatcg gtcaacacgc catcgacgtc gaaaatcatc
      421 agccggactt gccgggcctg agtcatggcg gcagtgctga atcggaccgt catcagatca
      481 ccttggcgcg agtcaaatcg tggatatgca acgcgccgac caatgttcct tgcgcatcag
      541 ccaccagaac ctgattgatg cggaattctt ccatcaattg cacagcatcg accgccagtt
      601 gatccggcat gatggtgcgt ggaccgtgat gcatgacatc ggcgattgag aaccgggtga
      661 aatcttcacc acgctcgagt aggcggcgca gatcgccatc ggtgaagacg ccgatcgggc
      721 ggtattgtcc atcgaccacg gcggtcatcg ccatgccctt gcgcgtgatt tccatcaatg
      781 ccactgatag caaaacgtcg ggcgtgactg cgggaacggc atcgccggtg cgcatgacat
      841 cgcgcacgtg cgtcagcaag cggcgaccca aggcgccgcc ggggtgtgag cgtgcaaagt
      901 cttcttccat gaaaccgcgc gcgtccagca gcgataccgc cagcgcatcg cccatcgcca
      961 acatggcggt ggtgctggcg gtcggcgcca ggttcaacgt gcaggcttct ttactgacgc
     1021 tgatgttcag gtgaatcgcc gacaagcgcg ccagactgga atcgtggtta ccggtcatgg
     1081 cgatcagatg ggcgcccatg cgcttgatga taggcacgat ggccatcaat tcagcggttt
     1141 cacccgaatt ggagatagca atgaaaacat cattggccgc gaccatgccg aggtcgccat
     1201 gcgcagcttc cgccggatgc atgaacaggg cgggcgtgcc ggtcgaagca aaggtcgctg
     1261 cgatcttgcg gccgatatgg ccggatttgc cgatgccgga gaccaccacg cgacctttgc
     1321 actccagcaa catgccaacg gcgcgggcca acggctcgtc ggggctatcg gatatccggg
     1381 cttgcagcgc cagaatagcg tcagcctcga tgcgcagggt ttgacgggcg agactgagcg
     1441 cgcgtcgggc gctttcggga gagaaaacct ttggcaggat ttgatattcg gttgcactca
     1501 tgagtgcagt ataaatgaat tacgaaagca aaaaacttgc cagaccgaat cctacttttt
     1561 cttctttagc aatggcgcca gataatgtcc ggtgacgctt tccggattcc tggcgacttc
     1621 ttccggtgtg ccggtcgcga tgatcctgcc gccgccggca ccgccttccg ggccgaggtc
     1681 gatgatccag tccgcggtct tgatcacgtc cagattatgc tcaatgatga ccaccgtatt
     1741 gccttgatca cgcaggcgat ggattacctt gagcaacaga tcgatatcgt ggaaatgcag
     1801 gccagtggtc ggctcgtcca gtatatacag tgtgcgtcct gtatcacgtt ttgacaattc
     1861 cagcgacagc ttgacgcgct gggcttcacc gcccgatagt gtggtcgcgc tctgacccag
     1921 cttgatataa ccgaggccga catcgagcag ggtctgcaac ttgcgcgcga tcagcggcac
     1981 cggcaggaaa aacgcatgcg catcttccac cgtcatttcc agggtgtcgg cgatgttctt
     2041 gcccttatat tgcacctcta gcgtttcgcg gttgtagcgc ttgccgtggc aaacgtcaca
     2101 aggcacgtaa atgtccggca aaaagtgcat ctcgaccttg atcacgccat caccctggca
     2161 ggcttcgcag cggccgccct tgacattgaa ggagaaacgt ccagcgctgt agccacgttc
     2221 tttggcagcc ggcaccgtgg cgaacagatc gcggatcggt gtgaacagac cagtgtaggt
     2281 cgccggattg gagcgcggcg tgcggccgat cggcgcttgg tctaccgaga tcactttgtc
     2341 gaaatactcc aggccgctga ttgactcatg ttccgcaggc ccggtttggg agccgtacag
     2401 atggcgcgcc gccgccagat agagcgtgtc gttgaccagc gtcgacttgc ccgaacccga
     2461 gacgccggtc acgacggtga gcgcgcccgt cggcacacgg acgtcgatgc ccttcaggtt
     2521 gtgctggcgg gcgccgatga cctcgatgca ggatgcgtcc atgccgaagg tgcctcagtg
     2581 gaccggacgg ggacgcgcgt gcgcgcgacg gcccgtgacc gg
//
LOCUS       DASRDH010000218         2187 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1215715,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000218 DASRDH010000000
VERSION     DASRDH010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1215715"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="VFA14_05030"
     CDS             <1..245
                     /locus_tag="VFA14_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HZS80772.1"
                     /translation="FAYWSHRIALLRGRMPVSKSLPYQWYDTPNLRCATIRDFEELAG
                     EVGLEVLERVALHEGVVVSFLLNWRGSLAVFRLKKK"
     gene            265..1527
                     /locus_tag="VFA14_05035"
     CDS             265..1527
                     /locus_tag="VFA14_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008115314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmpG family muropeptide MFS transporter"
                     /protein_id="HZS80773.1"
                     /translation="MIPSPISWRSWFNRRMLICVFLGFSSGLPLFILLSLLQAWLAKS
                     GLDVKALGLFALVMFPYTWKFLWSPLMDRFHFGRMGRRRGWMFFTQAALFLGIGAMGM
                     LDPHTQVATIAFMASVVAFLSASQDIVIDAYRREILPEEELGLGSVIHVNAYKLSGMV
                     PGALSLVLADLMPWQVVFWITAAFMLPGLLCTLAVKEPEIYGLPPKNLREAVVLPFQE
                     FVKRGSRGMAVLAFIFLYKLGDSMATALATKFYIDLGFSMTQIGVISKTSSLWASVLG
                     GIVGGIWMMRLGINKALWIFGVTQAVAILGFAWLVRIGPDALALAVVVGFEAFGVGLG
                     TAAFVAYIMRETDPRYTATQYALFTSLAAVPRTFINSSVGYIVADLGWFNFFILCFVL
                     TLPGMLMLIKIAPWNGETKVIFSADQSE"
     gene            complement(1499..>2187)
                     /locus_tag="VFA14_05040"
     CDS             complement(1499..>2187)
                     /locus_tag="VFA14_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HZS80774.1"
                     /translation="GLDGAFNMLLLARERRQMAQSTIDRLLTRWLASAGPGSAGGLVC
                     SDFARLPFAAHALDMVWSNLALHWHPQPDRVFTEWHRVLQVEGLLMFSCFGPDTFREL
                     RIAFERIDAVPHSLPFVDMHDLGDMLVHAGFSAPVMEMENITVTYDTPSHLLTDLRSW
                     GGNPLESRRRGLLGRAAHTRLLNALEQLRAVGGKIPLTFEVIYGHAFRPLPRTTPSVE
                     SIIHFDLPKK"
BASE COUNT          441 a          593 c          637 g          516 t
ORIGIN      
        1 acttcgccta ctggtcgcat cgcatcgcgc tgctgcgggg gcgcatgccg gtatcgaagt
       61 cattgcctta ccaatggtac gacacgccca acctgcgttg cgccaccatc cgggacttcg
      121 aggaactagc cggcgaagtc ggtcttgagg tgctagagcg tgtggcgttg catgaaggag
      181 tggtggtttc tttcctgctg aactggcgcg gcagtctagc agtattccgc ttgaaaaaaa
      241 agtagcgatg aaagcccagt atttatgatt ccttccccaa tttcgtggcg tagctggttc
      301 aaccgtcgca tgctgatctg tgtgttcctc ggtttttcgt cgggcttgcc tttgttcatt
      361 ctgttgagcc tgttacaggc atggctggcg aagtccggac ttgacgtcaa agcgctggga
      421 ctgttcgcac tggtgatgtt tccctacacc tggaaatttc tgtggtcgcc gttgatggac
      481 cggtttcatt ttggcaggat ggggcgtcgc cgcggctgga tgttctttac gcaggcagcg
      541 ctgtttctcg gcatcggcgc catggggatg ctggatccgc atacacaggt cgcaacgatt
      601 gcctttatgg cctcggtcgt agcctttttg tcagccagtc aggacatcgt gatcgacgcc
      661 tatcgtcgcg aaatcctgcc ggaagaagag ttggggctgg gcagcgtcat ccatgtcaac
      721 gcctacaaac tctccggcat ggtaccaggc gccttgtcac tggtgctggc cgacctgatg
      781 ccgtggcagg tggtattctg gattactgct gccttcatgc tgccgggttt actatgcact
      841 cttgccgtca aagagccgga aatctatggt ttgccgccaa aaaatctgcg cgaagccgtg
      901 gtgctgccat ttcaagaatt cgtcaagcgt ggcagtcggg gcatggcggt actagccttt
      961 atctttttgt acaagcttgg cgacagtatg gcgacggcgc tggcaaccaa gttttatatc
     1021 gatcttgggt tttcgatgac gcagatcggt gtgatttcca aaacaagcag cttgtgggcc
     1081 agcgtgctgg gtggcatcgt cggcggcatc tggatgatgc ggttgggtat caacaaagct
     1141 ttatggattt tcggtgtgac ccaggcagtc gccattctgg gcttcgcctg gctggtccga
     1201 atcggaccag atgcgttggc gctggccgta gtggtcggtt tcgaagcgtt cggcgtgggg
     1261 ctgggcacgg ctgctttcgt cgcttacatc atgcgcgaaa cggacccgcg ctacacagct
     1321 acccaatatg cgctgttcac cagtctggcg gcggttccgc gtactttcat caattcctcg
     1381 gtcggctata tcgtagcgga cctgggctgg ttcaatttct tcatcctgtg ttttgtactg
     1441 acgttgccag gcatgctgat gctgataaag atcgccccat ggaatggtga gaccaaggtt
     1501 attttttcgg cagatcaaag tgaatgatcg attccacgct cggcgtggtt ctgggtaatg
     1561 gacggaaagc atgaccatag atcacttcaa acgtcagcgg tatcttgcca ccgacagcgc
     1621 gcagttgctc cagcgcgttc agcaaacgcg tatgagcagc gcggcccagc agaccacgcc
     1681 gtcggctttc cagcggatta ccaccccagg agcgcaagtc cgtcaacagg tgtgacggcg
     1741 tgtcataggt tacggtgata ttttccatct ccatcactgg cgccgaaaag ccagcatgga
     1801 ccagcatgtc accaagatca tgcatgtcga cgaagggcag gctgtgcggc acagcatcga
     1861 tgcgctcgaa ggcgatacgc agctctctga aagtgtctgg tccgaaacag gaaaacatca
     1921 gcaaaccctc aacttgcaaa acccggtgcc actcggtgaa aacgcgatct ggctgcggat
     1981 gccagtgcaa cgccagattg gaccacacca tgtccaaagc atgcgcggca aacggcaacc
     2041 gggcaaagtc cgagcaaacc agccctccgg cactgcccgg gccggctgac gctagccatc
     2101 tggtcaacag acgatcgatg gtggattgcg ccatctgccg gcgctcccgc gccagaagca
     2161 acatattgaa ggcaccatcc agaccga
//
LOCUS       DASRDH010000219         3178 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_577257,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000219 DASRDH010000000
VERSION     DASRDH010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_577257"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3116
                     /locus_tag="VFA14_05045"
     CDS             <1..3116
                     /locus_tag="VFA14_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017232827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MdtB/MuxB family multidrug efflux RND
                     transporter permease subunit"
                     /protein_id="HZS80775.1"
                     /translation="AILLTGIVAYRQLPLSALPEVDYPTIQVVTLYPGASPDVMTSSV
                     TAPLERQFGQMPGLKQMSSASSGGASVITLQFNLDLSLDIAEQEVQAAINASGNLLPS
                     DLPAPPIYSKVNPADTPVLTLAVTSRTLPLPRVQDLIDTRLAQKISQLSGVGLVSLSG
                     GQRPAVRMQLNPRAVAALGMNLDDVRAAIGNASVNQAKGSFDGPARASTIDANDQLKS
                     ADEYRNLIIGYRNGAPIRVSDIADVVDAAENVRLGAWADNDAAVIVNIQRQPGANVIA
                     VVDSIKKILPTLQASLPGAIDVRILTDRTTTIRASVDDVRFELLLSIVLVVMVIFIFL
                     RNIPATIIPSVAVPLSLVGTFGVMYLAGFSINNLTLMALTIATGFVVDDAIVMIENIS
                     RYIEQGIAPLQAALKGAQQIGFTIISLTFSLIAVLIPLLFMGDVVGRLFHEFAVTLAA
                     AILISAVVSLTLTPMMCARLLRHVPQAEQGWFYHRSGVFFDGIIARYGVMLQWVLDHQ
                     RATLAVALGTLGLTVLLYIFVPKGFFPVQDTGVIQGISEAAQSVSFAAMAERQQAIAR
                     VVLADPAVESLSSFIGVDGVNTTLNSGRMLINLKPHAERDISASDVIRRLQPRLSAQV
                     PGITLYMQPVQDLTIEDSVSRTQYQFTLEDASTDALSVWVPKLVARLRQLPQLADVAS
                     NAQDQGLQAYVEIDRDTASRMGITTAAIDNALYNAFGQRLISTIYTQSSQYRVVMEVK
                     PEFQKGPDALNSIFLVAANGKQIPLASIARVSERTAPLVVNHLGQFPSATVSFNLVQG
                     AALGEALEAIAAAERDVGLPASMSTSFQGAALAFQASLSHTLWLILAAIITMYIVLGV
                     LYESTIHPVTILSTLPSAGVGALLSLMISRSDLGIIGIIGIILLIGIVKKNAIMMIDF
                     ALEAERNEGKSPREAIYQACLLRLRPILMTTMAALLGALPLMLGSGVGSELRQPLGIT
                     MVGGLLLSQALTLFTTPVIYLYFDRLSRRARGESTDQTQPRVRTALERETEIRTGGDS
                     ETEGSTP"
BASE COUNT          567 a         1016 c          957 g          638 t
ORIGIN      
        1 tggcgattct gctgaccggc atcgtcgcct ataggcaact gccgctgtcg gctttgccgg
       61 aagtcgatta tccgaccatc caggtcgtga cactgtatcc cggcgccagc ccggacgtga
      121 tgacctcctc ggtgacggca ccgctggaac gccagttcgg ccagatgccg ggtctcaagc
      181 agatgtcctc ggccagttcc ggcggtgcat cggtgattac gctgcaattc aatctcgacc
      241 tcagtctcga catcgccgag caggaagtgc aggccgccat caatgccagt ggcaatctgc
      301 tgccgtcgga cttgccggcg ccgcctatct atagcaaggt caaccccgcc gataccccgg
      361 tgttgacgct ggcggtgacg tccagaacgc tgcccttgcc cagggtgcag gacttgatcg
      421 acacgcgcct ggcgcaaaag atttcgcaac tctccggtgt tggtctggtc agtctgtccg
      481 gcggccaacg tccggccgtg cgcatgcagc tcaatccgcg tgcggtcgct gcgctgggca
      541 tgaacctgga tgacgtccgc gctgccatcg gtaacgccag cgtcaatcag gccaagggca
      601 gtttcgatgg cccggcgcgc gcctcgacca tcgacgccaa tgaccagctg aagtcggccg
      661 acgaataccg caatctcatc atcggctaca ggaatggcgc gccgatccgc gtgtctgaca
      721 tcgccgatgt ggtcgatgcg gcagagaatg tccgtcttgg cgcctgggct gacaacgacg
      781 ctgccgtgat cgtcaacatc cagcgccagc ccggcgccaa tgtcatcgcc gtggtcgata
      841 gcatcaagaa aattctgccg acgctgcaag ccagcctgcc cggcgccatc gacgtgcgca
      901 tcctgactga ccgtaccacg accatccgcg cctcggtgga tgacgtcagg ttcgaactgc
      961 tgctgtcgat cgtgctggtg gtaatggtga tcttcatctt tctgcgcaat attccggcga
     1021 ccatcatccc cagcgtcgcc gtgccgctgt cactggtcgg cactttcggc gtcatgtatc
     1081 tggccggctt ttcgatcaat aacctgacgc tgatggcgtt gacgattgcg actggcttcg
     1141 tggtcgatga cgccattgtg atgatcgaga atatctcacg ctacatcgaa cagggcatcg
     1201 cgcccttgca ggcagcgctg aagggcgcgc agcaaatcgg ctttaccatc atttcgctga
     1261 ctttttcgct gatcgccgtg ttgattccgc tgttgttcat gggggatgtg gtggggcggc
     1321 tgttccatga attcgccgtc acgctggcgg cggcgattct gatctccgcc gtcgtatcgc
     1381 tgacgctcac accaatgatg tgcgcgcgtc tgctgcgcca tgtgccgcaa gcggaacaag
     1441 gctggttcta ccacaggagc ggcgtcttct tcgatggcat cattgcgcgc tatggcgtga
     1501 tgctgcaatg ggtactcgat catcagcgcg ccacgctggc agtggcgctg ggcacgctgg
     1561 ggctgactgt gctgctgtac atcttcgtac ccaagggatt cttcccggtg caggataccg
     1621 gtgtgatcca gggcatttcg gaagcggcgc agtcggtgtc cttcgccgcc atggccgagc
     1681 gccagcaggc catcgccaga gtggtgctgg cagatccggc ggtcgagagc ctgtcgtcct
     1741 tcattggtgt cgatggcgtc aacaccacgc tcaacagcgg ccgtatgctg atcaacctga
     1801 agccgcatgc ggaacgcgac atcagcgcca gcgacgtcat ccgccgcctg caaccccgac
     1861 tgagcgcgca agtccctggc atcacgctgt acatgcagcc ggtgcaggat ctgaccatcg
     1921 aagacagcgt cagccgcacg caataccagt tcacgctgga agacgccagc accgacgcac
     1981 tcagcgtctg ggtgccgaag ctggtcgcgc gtctgcgcca gttgccgcaa ctggctgatg
     2041 ttgccagcaa tgcgcaggat caaggtttgc aggcgtatgt cgagatcgac cgcgacactg
     2101 cttcacgcat gggtattacg actgccgcca tcgataatgc cttgtataac gccttcggcc
     2161 agcgcctgat ctcgaccatc tatacgcaat ccagccagta ccgcgtggtg atggaagtca
     2221 agccggaatt ccagaaaggc ccggacgccc tcaacagcat tttcctggtc gccgccaacg
     2281 gcaagcagat tccgctggcc agcattgcca gagtgagcga gcgtactgcg ccgctggtgg
     2341 tcaaccacct cggccagttc ccgtcggcga cggtgtcgtt caacctggtg cagggcgcag
     2401 cgctgggcga ggcgctcgag gccatcgcgg cggcggagcg ggacgtcggt ctgccggcca
     2461 gcatgtccac cagctttcag ggcgcggcgc tggccttcca ggcatcgctg tcgcatacgc
     2521 tgtggctgat cctggcggcg atcatcacca tgtatatcgt gctgggcgta ctctacgaaa
     2581 gcaccatcca tccggtcacg atcctgtcga ccttgccgtc ggcgggcgtg ggcgctttgt
     2641 tgtcgctgat gatttcgcgc agcgaccttg gcatcatcgg catcatcggc atcattctgt
     2701 tgattggcat cgtcaagaag aacgccatca tgatgatcga tttcgcgctc gaagcagagc
     2761 gcaacgaagg caaatcgccg cgtgaagcga tctaccaggc ctgtctgctg cgcttgcggc
     2821 cgattctgat gactaccatg gcagctttgc ttggcgcctt gccgctgatg ctcggcagcg
     2881 gggtcggctc cgaactgcgc cagccgctgg gcatcaccat ggtcggcggc ctgctgctgt
     2941 cgcaggcgct gaccctcttc acgacgccgg tcatctacct ttacttcgac cgcctgtcgc
     3001 gccgcgcgcg cggcgaatcg accgaccaga cccagccccg cgtccggacc gcgctcgaac
     3061 gcgagactga aatcaggacc ggcggcgatt cagaaacaga gggcagcacg ccgtgaacat
     3121 ttccgagccc ttcatccggc gtcccgtcgc gacgacgctg atcaccctcg gcgtgacg
//
LOCUS       DASRDH010000220         2126 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_268532,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000220 DASRDH010000000
VERSION     DASRDH010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268532"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="VFA14_05050"
     CDS             complement(<1..212)
                     /locus_tag="VFA14_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008329681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZS80776.1"
                     /translation="MTKKNSAAIKVAIADDHAIVREGLKQICNSTRDIVVVGSAENGL
                     EAIKLCRLDNCDVLLLDIVLPDRSGI"
     gene            complement(230..916)
                     /locus_tag="VFA14_05055"
     CDS             complement(230..916)
                     /locus_tag="VFA14_05055"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HZS80777.1"
                     /translation="MRDSTLPGQQNHASVERMKEAERERIARDLHDELGSRLSAIKMA
                     VTQFGRRQSSHDTTLQKQMQYVTQLVDDAIDAMRDIIDDLHPTVLDLGLPAALEWLAQ
                     VFTRHTGMKYRLLGDDDLPDSLLDAFQTVSLYRIAREALHNASRHASIRNVVIGLHYL
                     DTRLTMEIIDDGVGLPADAASRAESSGIRGMQRRAAAIGATLTLQAAENNGVKLHVEL
                     PGLAGASPVN"
     gene            1100..1176
                     /locus_tag="VFA14_05060"
     tRNA            1100..1176
                     /locus_tag="VFA14_05060"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1134..1136,aa:Arg,seq:acg)
     gene            1413..>2126
                     /locus_tag="VFA14_05065"
     CDS             1413..>2126
                     /locus_tag="VFA14_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZS80778.1"
                     /translation="MQKKVFIKTFGCQMNEYDSDKMADVLNVSDGLVKTDKPEEADVI
                     LLNTCSVREKAQEKVFSDLGRLRELKKINPDLLIGVGGCVAAQEGAAIVKRAPYVDMV
                     FGPQTLHRLPQMINERRCTGRSQIDISFPEIEKFDHMPPARVEGATAYVSIMEGCSKY
                     CSYCVVPYTRGEEVSRRFEDVLTEVAGLAAQGVKEITLLGQNVNAFRGAMADGGIADF
                     ALLIEYIADIPGIERIRFVT"
BASE COUNT          434 a          579 c          634 g          479 t
ORIGIN      
        1 tcgatgccgc tacggtccgg cagcacgatg tccagcagca gcacgtcgca attgtcgagg
       61 cggcacagtt tgatggcttc cagtccgttt tcggcactgc cgacgacaac gatatccctg
      121 gtgctgttac agatctgttt aagaccttcg cgcacgatcg cgtgatcgtc ggcgatggcg
      181 actttgatgg cggcactgtt ttttttcgtc atagtcttga agacagatgc tagttgacag
      241 gactggcgcc tgccaggccg ggaagttcga catgaagttt cacaccgttg ttttccgccg
      301 cttgtagcgt cagcgtcgcg ccgatggcgg cggcacggcg ctgcatgcct ctgatgcccg
      361 acgactcggc ccgactggca gcgtcggccg gcaggccgac gccgtcatcg atgatctcca
      421 tcgtcagccg cgtgtcgagg taatgcaggc cgatgacgac gttgcggatg gaggcatgcc
      481 ggctggcgtt gtgcaatgct tcgcgggcga tgcggtacag gctgacagtc tggaaggcat
      541 cgagcaggct atccggcaag tcgtcgtcac cgagtagacg atacttcatg ccggtatggc
      601 gggtaaatac ctgggccagc cattcgagcg cggcgggcaa gcccagatcc agtactgtgg
      661 ggtgcaggtc gtcgatgatg tcgcgcatgg cgtcgatggc gtcatcgacc agttgtgtga
      721 catattgcat ctgtttctgc aaagtggtgt cgtgtgacga ctggcgccgg ccgaactgcg
      781 tcacggccat tttgatcgcg ctcaaacgac ttcccagctc gtcgtgtaaa tcccttgcga
      841 tacgctcccg ctcggcttct ttcatgcgtt ccacggaggc atggttctgt tgtccaggta
      901 gggtgctgtc acgcatatcg gcaagtgtct cagtgataat caagcaaacc ggtatcagta
      961 gatgggtttg ccggcaagat ggggagtggt ataagtgccg aataaaagtg ctgaaaaatc
     1021 aatattcagc catgctcgag aatttttgaa actacgccct tccatttcgt gaaaatgtga
     1081 tgctataatg cctgctttcg cggctgtagc tcagctggat agagtacttg gctacgaacc
     1141 aaggggtcgt gggttcgatt cctgccagcc gcaccagatg cgaagggcca cactacagga
     1201 tgtgggtccg acatgtcaat aagcccccgg tttttatcgg gggcttattg tttggtatgt
     1261 ccggcatggc cgctgcccgc aggtcggtcc gcgacatccg gtgctacaat cacaaacttt
     1321 attccctgcg ccgaaacagc aggaccggcg ttcttcctgc cggcactgac gatgatgtat
     1381 cgccgccgtg tcttcgcgcc tgaatcacat ttatgcagaa aaaagtattc atcaaaacct
     1441 tcggttgcca gatgaatgag tatgactcgg acaagatggc cgatgtactc aacgtctcag
     1501 acggactggt caagactgac aagccggaag aggctgatgt catcctgctg aatacctgct
     1561 cggtgcgcga gaaggcgcag gagaaagtgt tttccgacct cggtcgcctg cgcgagctga
     1621 agaaaatcaa tcccgatctg ctgatcggtg tcggcggctg tgtggctgcg caagaaggcg
     1681 cggccatcgt caagcgcgcg ccttacgtcg atatggtgtt cggtccgcaa accttgcacc
     1741 gtctgccgca gatgatcaac gaacgtcgct gcaccggtcg ctctcagatc gacatcagct
     1801 tccccgaaat cgaaaaattc gatcacatgc cgccggctcg cgtcgaaggc gcgactgcat
     1861 atgtgtcgat catggaaggt tgcagcaagt attgcagcta ctgcgtggtg ccgtatacgc
     1921 gcggcgaaga agtgtcgcgc cgtttcgaag acgtgcttac cgaagtggcc ggactagccg
     1981 cgcagggcgt caaggaaatc acgctgctgg gccagaacgt caatgccttc cgcggcgcaa
     2041 tggccgatgg cgggattgcc gacttcgcgc tgctgatcga atacattgct gatattcctg
     2101 gcatcgagcg catccgcttc gtcacc
//
LOCUS       DASRDH010000221         4829 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_503514,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000221 DASRDH010000000
VERSION     DASRDH010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 4829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4829
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503514"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..119
                     /locus_tag="VFA14_05070"
     CDS             <1..119
                     /locus_tag="VFA14_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminomethyltransferase beta-barrel
                     domain-containing protein"
                     /protein_id="HZS80779.1"
                     /translation="GTRFDLRFADPQWAVTPGQSAVLYQGDVCLGGGIIEST"
     gene            213..401
                     /locus_tag="VFA14_05075"
     CDS             213..401
                     /locus_tag="VFA14_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007878681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HZS80780.1"
                     /translation="MNQSIGQQTKPRNSSGAAAHGSSIARVKSQDLLRQMRELEIDHG
                     GRIYTLRLTQLNKLILTA"
     gene            424..744
                     /locus_tag="VFA14_05080"
     CDS             424..744
                     /locus_tag="VFA14_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006461713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS80781.1"
                     /translation="MATDNMDFAFVEPNGAPAQPELLVSAVLHLMSHYAAGNQESGNC
                     VKLASVIERHLKALADLPNLAPVLRATCRQLSEQWAIVVERAMPQPEKFRLFARIIPG
                     AKVG"
     gene            767..1003
                     /locus_tag="VFA14_05085"
     CDS             767..1003
                     /locus_tag="VFA14_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013232729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HZS80782.1"
                     /translation="MIVCICNNVSEGQIQQAVDAGIRSMSELRKELGVAACCGKCHTC
                     AKRILGECLGNTSQMRHPVQVIAFHANAAKSRRP"
     gene            1000..1656
                     /locus_tag="VFA14_05090"
     CDS             1000..1656
                     /locus_tag="VFA14_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007878668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HZS80783.1"
                     /translation="MSRSFACRADAVQRAIRKTGLLGLIILLHIACFLVLRNALVVHQ
                     PVNVIPKEVIVSLITPEHTPEPRPSQAPKAVQAGRKSFAPAPMPVTRTSAPTAINTPS
                     APAEEPSLPAAPAAASPPGPPQPKQITSGIEYIEAPRPAYPAASKRMGEEGVVRFRVL
                     VNTAGKAEKVDIIHSSGSSRLDEEVRRAVMRAVFKPHIENGETITVSADGVITFKLNR
                     "
     gene            1725..2459
                     /locus_tag="VFA14_05095"
     CDS             1725..2459
                     /locus_tag="VFA14_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007878665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HZS80784.1"
                     /translation="MEMNPYGLQSLWAQGDFVTKGVAALLVTMSIASWYVIVTKALQL
                     LRFRRASSTAGHAFWDATSLPEGVAMLGTDNPFTEVAKAGAASMAHHTAHEGHLHDQL
                     SVSDWVTLSLRQAIDDASGKLQTGMAVLASVGSTAPFVGLFGTVWGIYHALVSIGTSG
                     QASIDKVAGPVGEALIMTALGLAVAIPATFGYNALVRGNKSTIAKLNKFGFDLHALFV
                     TGARAATEDTETGAAKNTKLTAVRRA"
     gene            2459..2875
                     /locus_tag="VFA14_05100"
     CDS             2459..2875
                     /locus_tag="VFA14_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HZS80785.1"
                     /translation="MAMGSLSDADDNFNPEINTTPLVDVMLVLLIIFIITIPVMNHAV
                     KIDLPRAVSQPDAGRLQNIDLSIDARGQLFWNEELIDSNQLSLRIAEAAKRQPQPELH
                     LRAERTTQYEKIAQVMAAAQSGGLTRIGFVTDPKIR"
     gene            3070..3543
                     /gene="bfr"
                     /locus_tag="VFA14_05105"
     CDS             3070..3543
                     /gene="bfr"
                     /locus_tag="VFA14_05105"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007878654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HZS80786.1"
                     /translation="MKGDPAIIKLLNAQLTNELTAINQYFLHARMYKHWGLEKIAKKE
                     FAESIGEMKHADQLINRILMLDSLPNLQTLHKLMIGENITEILQCDLKLEKAAQKTVK
                     GGIASCETHGDYVSRELFQMILDDTEEHIDWLETQLDLIEKIGIQNYLQAQTIAE"
     gene            complement(3578..3982)
                     /gene="dksA"
                     /locus_tag="VFA14_05110"
     CDS             complement(3578..3982)
                     /gene="dksA"
                     /locus_tag="VFA14_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006464880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HZS80787.1"
                     /translation="MLTEKQILKMSEKDYMNEAQLAFFKARLQQLEKDLLKNAGETTE
                     HLRETVLVPDPADRATIEEEHALELRTRDRERKLLKKVQQSIIAIDAGEYGWCEETGE
                     PIGIPRLLARPTATLSLEAQQRRELKQKLYGD"
     gene            complement(4394..>4829)
                     /locus_tag="VFA14_05115"
     CDS             complement(4394..>4829)
                     /locus_tag="VFA14_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HZS80788.1"
                     /translation="DDVHLGELVRSATVVLDCSDNFVTRHAINRACVACKVPLVSGAA
                     IRFDGQVSVFDARRSDAPCYACLFPPDQQFEEVQCSTMGIFSPLVGIVGAMQAAEAIK
                     LVAGIGASLAGRLQTIDARSMEWSSIRLGRNPVCLVCGATSV"
BASE COUNT         1114 a         1444 c         1269 g         1002 t
ORIGIN      
        1 caggcacacg tttcgacctg cgttttgccg atccgcaatg ggcagtcact cccggccagt
       61 ctgctgtgct gtatcaaggc gatgtctgcc tgggcggcgg catcatcgaa tccacctgaa
      121 aacatgcgcc aaatcatgaa aaagcttgca atcccggcaa agatgacaaa taatctaaac
      181 aggaattgtt cttattcaac aggagatgaa cgatgaacca atcgatcggg cagcaaacca
      241 aacctcgtaa cagctcgggt gcagcagcgc atggctcctc tattgcgcgc gtcaaaagcc
      301 aggatttgtt gcgccagatg cgtgagctgg aaatcgacca cggcggccgc atctacacac
      361 tacgcctgac gcaattgaac aagctgatcc tgacggcgtg aactgccgac caagaaaagt
      421 ctgatggcga ccgataacat ggattttgcc ttcgtggaac cgaatggcgc gccggcacag
      481 ccggagctgc tggtttcagc agtgctgcac ttgatgtcgc attacgctgc cggcaatcag
      541 gaaagcggaa actgcgtcaa actggcctca gtgatcgagc ggcacctgaa agccttggcg
      601 gatttaccga atctggcgcc ggtattacgg gcgacttgcc ggcaattgtc ggaacagtgg
      661 gccatcgtcg tggaacgtgc catgccgcaa cctgaaaagt tcaggctgtt cgcgcgcatc
      721 atcccgggcg cgaaggtcgg ctaaggctgt taccacagag catgccatga tcgtctgtat
      781 ttgcaataac gtttccgaag gccagattca gcaggccgtc gatgcaggca tacgttccat
      841 gtccgaactg cgcaaggaac tcggcgtcgc tgcctgttgc ggcaaatgtc atacctgcgc
      901 caagcgcatc ctgggcgaat gtctcggcaa caccagtcaa atgcgccatc cggtacaggt
      961 catagcgttc catgcaaatg ccgccaaatc tcgccgccca tgagcaggag tttcgcctgc
     1021 cgtgccgatg ccgtgcagcg tgcaatcagg aaaaccggcc tgctgggatt gatcatcctg
     1081 ctgcacattg cttgctttct tgtactacga aacgctctgg tggtccatca gcccgtcaac
     1141 gtcatcccga aagaagtcat cgtcagcctc atcacgcctg agcacacacc agaaccgagg
     1201 ccgtctcagg cgccgaaagc cgttcaggcc ggcaggaaat ccttcgcgcc ggcaccgatg
     1261 cctgtgaccc gaacatctgc ccccaccgcc atcaataccc cgtcggcacc ggcagaagaa
     1321 ccgtcgctgc ccgccgcccc tgctgcggca tcaccaccgg ggccgccgca gccaaagcaa
     1381 atcacttccg gtatcgaata catcgaagcg ccgcgccccg cttatcccgc cgcttccaaa
     1441 cggatgggag aagaaggcgt cgtgcgcttc cgcgttctgg tcaacaccgc cggtaaagcc
     1501 gaaaaagtcg atatcattca cagttcggga tcatcccgcc tcgatgaaga ggtccgacgc
     1561 gctgtcatgc gcgccgtctt caagccgcac atcgaaaatg gcgaaaccat caccgtatcg
     1621 gccgacggag tcatcacatt caagctgaat cgctgattcc ggtatgcctg cgaaacgata
     1681 tgcgtcatcg atccattcat tatcgtcatc aaaaggaaag catcatggaa atgaatccat
     1741 atggattgca aagcctgtgg gcgcaaggag atttcgtgac caaaggcgtc gcggcactgc
     1801 tggtcaccat gtcgatcgcc tcttggtatg tcatcgtcac gaaggcgctg caactgttgc
     1861 gctttcgccg cgcctccagc accgccggtc acgcattctg ggacgccacc agtctgccgg
     1921 aaggcgtcgc catgctaggc accgacaacc cattcaccga agtcgccaaa gccggtgccg
     1981 cttcaatggc tcatcacacc gcgcacgagg ggcatctgca cgatcagttg tcggtcagcg
     2041 attgggttac gctgtcgctg cgccaggcta tcgacgatgc ctccggcaag ctgcaaaccg
     2101 gcatggccgt actggcatcg gtcggctcca cagcaccgtt cgtcggtctg ttcggcactg
     2161 tctggggcat ctatcatgcg ctggtatcga tcggtacttc cggccaggcc agcatcgaca
     2221 aggttgctgg tccggtagga gaagcgctga tcatgacggc gttgggtctg gctgtggcca
     2281 ttcccgccac cttcggttac aacgccctgg tgcgcggtaa caagagtacc atcgctaaac
     2341 tcaacaaatt cggcttcgat ctgcacgccc tgttcgtcac cggtgcccgc gcggcgactg
     2401 aagatactga aaccggcgcg gcaaagaata ccaaactgac agctgtcagg agagcatgat
     2461 ggcaatgggt tccctgtccg atgcggacga caatttcaat ccggagatca acaccacgcc
     2521 gctggtcgac gtgatgctgg tgctgctgat catcttcatc atcaccattc cggtcatgaa
     2581 tcacgccgtc aagatcgact tgccgcgcgc cgtaagccag ccggacgcag gcagactgca
     2641 aaacatcgat ctgtcgatcg acgcccgtgg ccagctgttc tggaatgagg aactgatcga
     2701 cagcaatcaa ctgagtctgc gtatcgccga agcggctaaa agacaaccac agccggaatt
     2761 gcatctgcgt gccgagcgca cgacgcaata cgaaaaaatc gcgcaagtca tggcggccgc
     2821 ccagtcgggc ggactcacca gaattggctt cgtcactgac ccaaaaatca ggtaaccgtc
     2881 cgttgaggcg gcacatgctc cgcctcgttc ttggcatgat tgcaagaaaa taaagtaaga
     2941 atgatttgcg ctaatttatg ctgaagtcca gcatcgactg cgttatgatc cgtccattta
     3001 cgcggctgga tccgcagacc tcatctgccc gcaccggccc tcccccctac accatgcccg
     3061 gaggtttcca tgaaaggcga tccagcgatc atcaagctgc tcaatgcaca attgacgaat
     3121 gagttgacag cgatcaacca gtattttctg catgcccgca tgtacaagca ctggggtctt
     3181 gaaaaaatcg caaaaaaaga gttcgctgaa tccatcggcg aaatgaagca tgccgaccaa
     3241 ctgatcaatc gcatcctgat gctcgacagc ctgcccaacc tgcaaacgct gcacaagctg
     3301 atgataggcg aaaacatcac cgagatactg caatgcgacc tgaaactgga aaaagctgca
     3361 caaaaaaccg tgaagggagg cattgccagc tgtgaaaccc acggcgacta tgtctcacgc
     3421 gaactgttcc aaatgatcct cgacgatacc gaggaacata tcgactggct ggaaacccag
     3481 ctcgacctga ttgaaaagat cggtatccag aattacctgc aagcgcagac catcgctgaa
     3541 taaagccagg cagttccgga attgctgcgg cgggaattca gtcgccgtac agcttctgct
     3601 tcagctcgcg acgttgctgc gcttccagag acaaggttgc ggtcggacgc gccagcaggc
     3661 ggggaatgcc gatcggttcg ccggtctctt cgcaccagcc gtattcaccg gcatcgatgg
     3721 caatgatgga ctgctgcacc ttcttgagca gcttgcgttc gcggtcacgg gtgcgcagtt
     3781 ccagcgcatg ttcttcctcg atcgttgcgc ggtcagcagg atcgggaacc aacactgttt
     3841 ctcgcagatg ctcagtggtt tcgcctgcat tcttcagcag atctttttcc agctgctgca
     3901 aacgggcctt gaaaaaagca agttgcgctt cattcatgta gtccttctcg ctcattttga
     3961 gaatttgttt ctcagtgagc aaggtctgcg tatcaagggt agttttggta gttttggtcg
     4021 ccacttcgct tactttcaat gccacggttt tttcgattct atcctttaat cctgcgcttt
     4081 cctgccgttt gtcaccttac cgttggattt gtgccgatgc cgcctttcca gtcatgtcat
     4141 cgattacctc gcccccgtgc tgtgccgggc gtctctggcg acatttattt aacggtcagg
     4201 gattttacta caacaacata ggctgtcggt gcagctgttt ttttcccgga catgacagct
     4261 tttttctgcc ggtttagccc cggcaacgac gctgtacagt gtataccaga acctggcgca
     4321 taaatagcac ttcgtcccct cgatgaaacg ctggcatgca gacgctcaca acatgttgac
     4381 agccgacaag ttctcagacg ctcgttgcgc cgcagaccag gcaaaccgga ttgcgcccca
     4441 accggatact gctccattcc atgctacgcg catcgatcgt ttgcaaacgg ccggccagtg
     4501 atgcgccgat gccggccacc agtttgatcg cttcagcagc ctgcatggcg ccgacaatgc
     4561 caaccagtgg cgaaaagatt cccatggtcg aacactgtac ttcttcgaat tgctgatcgg
     4621 ggggaaacag acaggcatag cacggcgcat cgctacggcg cgcgtcgaac acactgacct
     4681 ggccgtcaaa gcgaatcgcc gcgcccgaca ccagcggcac cttgcaggcg acacaggcgc
     4741 gattgatggc gtgacgggtc acaaagttat cgctgcaatc gagtaccacc gtagcgctac
     4801 gcaccagctc acccagatgc acgtcatcg
//
LOCUS       DASRDH010000222         4124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_38974,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000222 DASRDH010000000
VERSION     DASRDH010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 4124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4124
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38974"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..1217)
                     /gene="metK"
                     /locus_tag="VFA14_05120"
     CDS             complement(57..1217)
                     /gene="metK"
                     /locus_tag="VFA14_05120"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008112697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HZS80789.1"
                     /translation="MSNEYFFTSESVSEGHPDKVSDQISDAILDAVLAQDPLSRVAAE
                     TLCNTGLVVLAGEISTHANVDYIDVVRETIKRIGYDNADYGIDHKSCAVLVAYDKQSP
                     DIAQGVDEGKGLDLDQGAGDQGLMFGYACNETPELMPAAIHYAHRIVERQSQLRKDGR
                     LPWLRPDAKSQVTLKYVDGHPVAIDTIVLSTQHAPEMSHKAIEEAAIEDIIRPVVPAE
                     WLKNPRYLINPTGRFVIGGPQGDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDRS
                     AAYAGRYVAKNIVAAGLASRCQIQVSYAIGIARPTSVMVTTFGTGKISDEQLAQLVRE
                     HFDLRPKGIVQMLDLLRPIYQKTAAYGHFGREEPEFSWERTDKAAALRAAAL"
     gene            1440..2285
                     /locus_tag="VFA14_05125"
     CDS             1440..2285
                     /locus_tag="VFA14_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017449721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HZS80790.1"
                     /translation="MLFSLFRLLSLLPLPLLHLIGIVIGWLVYLVSPSYRKRLAENLT
                     RAGYGRYLCVAISESGKGIFELPFIWCASPRRVLRTARIENWQLAQQALDAKTGVVFL
                     TPHLGCFEIIAQAIAEQTSLTALYRPPRKAALKPLIEGARARPNLHLAPASLSGVRIL
                     LKALKAGKAIGLLPDQVPQYGEGVWADFFGKPAYTMTLSAKMHRMSGAPIILSYAERL
                     SWGRGFVIRFIAFEEALDDAPERQARAINQAMEKLIARCPAQYIWSYDRYKTPPGISA
                     PGAAQ"
     gene            2282..3163
                     /locus_tag="VFA14_05130"
     CDS             2282..3163
                     /locus_tag="VFA14_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007879403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis acyltransferase"
                     /protein_id="HZS80791.1"
                     /translation="MKALIILMWLLHWLPLPILGRLGAVLGFLLYFIMKPRRHITLTN
                     LRLCFPDKSEAEREIIARQHFQAYARSALERGVLWWASEARLRRLIRIEPHVPLEMIK
                     SSPTILLCPHFVCLEIPGIALVLNSDLSVCTLYSRQQNVVIDRALLKGRSRFRPVTLL
                     SREKGIKPIIRAMRAGLPFIMLPDMDFGLRDAEFVPFFGVPAATLTATARIAAATQAN
                     VIPMTTTFLPNYQGWKTTFYPAWENYPGDDISLAARRMNAFIEQRVLEAPAEYFWAHK
                     RFKTRPAGAPDVYGIND"
     gene            3190..4041
                     /gene="dapF"
                     /locus_tag="VFA14_05135"
     CDS             3190..4041
                     /gene="dapF"
                     /locus_tag="VFA14_05135"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007879398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HZS80792.1"
                     /translation="MKIRFTKMHGAGNDFIIIDATRQHIDLSQDQWKFIADRRFGIGA
                     DQVLIIEKPRSPGIDFRYRIYNADGGEVEQCGNGARAFARFVSDKGLTHKRVIRVETL
                     SGVIELTLEEDGRVKVDMGMPVWTPAQVPFDAAGLNSRVEGADALWPLDVGDKTSWVS
                     LVSMGNPHAVQVVADVEAAPVLSAGPLIECHPRFPKRVNAGFMQVVDQHHVKLRVFER
                     GAGETLACGTGACAAVVAGIRRGLLETPVTVQTRGGELVIAWAGSGWPVMMTGPAVSV
                     FEGEIEL"
BASE COUNT          797 a         1164 c         1238 g          925 t
ORIGIN      
        1 cgctttgcac gccctccttt cgtagaaaca tccggacgac cggatggctg aggaaattat
       61 aaagcggcag cgcgtagggc agcagccttg tcggtgcgtt cccatgagaa ttccggctct
      121 tcgcggccga aatgaccgta agcggcggtc ttttgataga taggacgcag cagatccagc
      181 atttgcacga tgcctttcgg acgcaggtca aaatgttccc gcaccagttg cgccagctgc
      241 tcgtcgctaa tcttgccggt accgaaggtg gtcaccatca ccgacgtcgg cctggcgatg
      301 ccgatggcat acgatacctg gatctggcaa cgcgatgcca gaccggcggc gacgatattc
      361 ttggcgacat aacggccggc atacgctgcc gaacggtcga ctttggatgg atccttgcct
      421 gagaacgcgc cgccgccgtg cggcgctgcg ccgccgtagg tgtcaacgat gatcttgcga
      481 ccggtcaggc cgcaatcgcc ttgcggaccg ccgatgacga agcgtccagt cgggttgatc
      541 aggtagcgcg ggttcttcaa ccattccgcc ggtacgaccg gacggatgat gtcttcgata
      601 gcggcttctt cgatggcttt atggctcatt tcaggcgcat gctgggtgga caacacgatg
      661 gtgtcgatgg cgaccgggtg tccgtcgacg tatttcagcg tgacctggga tttggcatcc
      721 ggacgcagcc atggcagacg gccatccttg cgcagctgcg actggcgttc gacgatgcgg
      781 tgtgcatagt ggatcgcggc gggcatcagt tccggcgttt cgttgcaggc ataaccaaac
      841 atcaagccct ggtcgccggc gccctgatcg agatccaggc ccttgccctc gtcaacgccc
      901 tgggcgatgt cgggcgactg tttgtcgtag gcgaccagca cggcgcagct tttgtggtcg
      961 atgccgtagt cggcgttgtc gtaaccgata cgcttgatgg tttcacggac gacgtcgatg
     1021 tagtcgacat tggcgtgtgt agagatttcg ccggccagca cgaccaggcc agtgttgcaa
     1081 agggtttcag cggcaacgcg cgagagcgga tcctgggcta ggacggcgtc cagaatcgcg
     1141 tcggaaatct ggtccgagac tttgtctggg tggccttcgg aaacggattc ggaagtgaaa
     1201 aaatactcgt tggacattgc aggctcctgc cgtagagaga tttaaccatg tcgcggtctg
     1261 tctcagagcc tgcgacgctt tagcgaaatt tatatcccgt ctcgcaagtt gtcattaact
     1321 cgacggcttt cgtgctattt tacgcatctt gtaaaaattc ggcaatttca caggatgatg
     1381 agagtgcatt ttatccattg gggaggatgg atggaatgtg ttccagatga atggcaagta
     1441 tgcttttttc tttattccga ctgctctcct tgcttccgct tcctttgctg cacctgatcg
     1501 ggattgtgat agggtggctg gtctatctgg tatcgccgtc gtaccgcaag cgtctggccg
     1561 agaatctgac ccgcgccggc tatgggcggt acttatgcgt tgccatcagc gagtcgggta
     1621 aaggcatctt tgaattgccg ttcatctggt gcgcgtcgcc caggcgcgtg ctgcgcacgg
     1681 cccgtatcga gaactggcaa ctcgcgcagc aggcgctgga tgccaaaacc ggcgtggttt
     1741 ttttgacgcc gcacctgggt tgctttgaaa tcattgcgca ggccattgct gagcagacat
     1801 cgttgactgc cctctatcgc ccgccgcgta aggcggcatt gaagccgttg attgaaggcg
     1861 cgcgcgctcg ccccaacctg catctggcgc ccgccagcct gtccggcgtg cgtatcttgc
     1921 tcaaggcgct gaaagccggc aaggccatcg gcctgttgcc ggatcaggtg cctcagtatg
     1981 gcgaaggcgt gtgggcggat tttttcggca aaccagccta caccatgacc ctgtcggcca
     2041 agatgcatcg gatgagcggc gcgcctatca ttttgtcgta tgctgaacgt ctgtcgtggg
     2101 gccgcggttt cgtgatccgc ttcatcgcct ttgaagaagc gctggacgac gcgccggagc
     2161 gacaggcccg cgccatcaac caggctatgg aaaagctgat cgcgcgctgc cccgcacaat
     2221 atatctggag ctatgaccgc tacaagaccc cacccggcat cagcgcacct ggagccgcgc
     2281 aatgaaagcg ctgatcatat tgatgtggct gctgcactgg ctgccgttgc cgatactggg
     2341 gcgactcggc gcagtcctgg gtttcctgct ctatttcatc atgaagccgc gccgccatat
     2401 cacgctgact aatctacggc tgtgttttcc agacaaatcg gaagcagaac gtgaaatcat
     2461 cgcccgccag catttccagg cctatgcccg cagcgcattg gagcgcggtg tcttgtggtg
     2521 ggcctcggag gcgcgcctga ggcgtttgat caggatcgaa ccgcatgtgc cgctggagat
     2581 gatcaaatcc agcccgacca ttctgctatg tccgcatttt gtttgcctgg aaattcccgg
     2641 catcgcgctg gtactcaatt ccgatctgtc ggtctgtact ctttattcca ggcaacaaaa
     2701 tgtagtcatt gaccgggcgt tgctcaaggg ccgctcgcgt tttcgtccgg tgacgctgct
     2761 atcgcgtgaa aaaggcatca aacccattat ccgtgccatg cgcgcaggct tgcctttcat
     2821 catgctgccg gacatggatt tcggcctgcg cgacgccgag ttcgtcccgt tcttcggcgt
     2881 accggcggcc acactgacgg ccaccgcacg cattgccgcc gcgacacaag ccaatgtgat
     2941 accgatgact acaactttcc tgccaaatta ccaaggctgg aaaaccacgt tctatcccgc
     3001 ctgggagaat tatcccggtg acgatatcag ccttgccgcg cggcggatga atgccttcat
     3061 cgagcagcgt gtgttggaag cgccagctga atatttctgg gcccacaagc gttttaaaac
     3121 ccgtccggcg ggcgcgcctg atgtatacgg cataaatgac tgagcgtatc ccctctatat
     3181 aattagcgta tgaaaatcag gttcaccaaa atgcatggcg ccggcaatga tttcatcatc
     3241 atcgacgcca cccgccagca catcgatctg agtcaggatc aatggaaatt tatcgccgac
     3301 cgccgttttg gcattggcgc cgaccaggtg ctgatcatcg aaaaaccgcg atcgccgggc
     3361 atcgattttc gttatcgcat ttataacgct gacggtggcg aagtcgagca atgcggcaac
     3421 ggtgcgcgcg ccttcgcccg tttcgtcagc gataaagggc tgacgcacaa gcgcgtcatc
     3481 cgtgtggaaa ccctgtccgg cgtcatcgaa ctgacgctgg aagaagatgg tcgcgtgaag
     3541 gttgacatgg gtatgccagt atggacgccc gcgcaagtgc cgttcgatgc agccgggcta
     3601 aacagcaggg tcgaaggcgc tgatgcgctg tggccgctgg atgtcggcga caagaccagc
     3661 tgggtatcgc tggtgtcgat gggcaacccg catgctgtgc aggttgtggc cgacgtcgaa
     3721 gcagcgccgg tgttgagcgc cggtccgttg atcgaatgtc atccacgttt ccccaagcgg
     3781 gtcaatgccg gcttcatgca agtcgtcgat cagcaccatg tgaaattgcg tgttttcgag
     3841 cgtggcgcgg gcgaaacgct ggcctgcggt actggcgcct gcgctgctgt cgtcgctggc
     3901 atacggcgcg gcttgttgga gacgccggta acggtgcaaa cccgcggcgg tgaattagtg
     3961 atagcctggg ccggctccgg ctggccggtc atgatgacag gcccggcggt gtcagtcttt
     4021 gaaggcgaaa tcgagctctg aacgagcgga tatttttccc gtgcagtaac atgatggcgg
     4081 ggacgacact ccgtcttccg atttaccgca agtcgtttca gccg
//
LOCUS       DASRDH010000223         5020 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_843665,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000223 DASRDH010000000
VERSION     DASRDH010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 5020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5020
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_843665"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="VFA14_05140"
     CDS             complement(<1..309)
                     /locus_tag="VFA14_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013234886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HZS80793.1"
                     /translation="MKEFPPAKLVIASRESRLAMWQSEYVRDRLVALYPQCSISILGM
                     TTRGDQILDRTLSRVGGKGLFVKELEVAMEEGRADCAVHSLKDMPMALPAGYTLAAIL
                     E"
     gene            473..3340
                     /gene="ppc"
                     /locus_tag="VFA14_05145"
     CDS             473..3340
                     /gene="ppc"
                     /locus_tag="VFA14_05145"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007881386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HZS80794.1"
                     /translation="MASKSHLPPLARAIASLPEKRTIKSADKDAPLKEDIRLLGRLLG
                     EVLRDQEGDAVFEIVETIRQTAVRFRRESDARAGVELDKLLRKLTRDQTNSVVRAFSY
                     FSHLANIAEDQHRNRRHRAHLLAGSTPQPGSIAYALSRLNAAGVSGGAVRDFLGTALI
                     SPVLTAHPTEVQRKSILDAECEIARLLTLRDNPLTPKELRDNTELLRARIATLWQTRM
                     LRFTRLTVADEIDNALSYYRITFLRALPALYDDIEEEIHTQFSSHAAPAELPPFVQMG
                     SWIGGDRDGNPNVSADTMQRALQRQSATIFDFYLDEVHTLGAELSISTFMVDASTALL
                     ALADASPDTSSHRADEPYRRALIGVYARLAATARALGVVNILRQEVGAAAPYGSPEEY
                     IGELQVLVDSLKAHHGGALVKPRLAVLKRSVEIFGFHLATLDMRQSSDVHERVLDELC
                     ASAQAEPSYAALSEQKKITLLLAELSRPRLLYSPYLHYSDETISELATLRMAHEIRRR
                     YGARAIRNYIISHTETLSDLLEVLLLQKETGLLHADANRQMTELELMVVPLFETIPDL
                     RRAAGIMAQWLALPQVAPLIARQGRLQEIMLGYSDSNKDGGFLTSNWELYKSANRLIE
                     LFNRADVRLRLFHGRGGTVGRGGGPSYQAILAQPPGTVNGQIRLTEQGEIIASKFSNP
                     EIGRRNLELLVAATLEASLMPHATDTKEARKLAEFEQVMEDLSERAYQTYRNLVYETP
                     GFTDYFFAATPIGEIAELNIGSRPAARKSMRRIEDLRAIPWGFSWGQCRLLLPGWYGF
                     GGAISGWLQEVGSKGRAKRLATLRAMYQEWPFFAALLSNMDMVLSKTDLAVASRYAGL
                     VSDHALRTAIFGRIADEHERSSRLLSAISGNQERLASNPLLARSIKNRFAYLDPLNHL
                     QVELISRRRSAVAAGRQIEERVQRGIHLTINGIAAGLRNTG"
     gene            3442..4860
                     /gene="argH"
                     /locus_tag="VFA14_05150"
     CDS             3442..4860
                     /gene="argH"
                     /locus_tag="VFA14_05150"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010401443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HZS80795.1"
                     /translation="MTTQLSKKSEAWSARFSEPVSELVKRYTASVFFDKRLAPFDIEG
                     SLAHAEMLAQQSIISARDYADIQRGMAQIKSEIDDGRFAWLPDLEDVHLNIEKRLTEL
                     VGDAGKRLHTGRSRNDQVATDIRLYLRAAIDGIDGLLRELRTALVDLAEQHADTIMPG
                     FTHMQVAQPVTFGHHILAYVEMFSRDAERMADARRRINRLPLGAAALAGTTFPIDRLR
                     VARTLGFDDVCRNSLDAVSDRDFAIEFCAAAALVMTHISRMSEELVIWMNPRLAFIDI
                     ADRFCTGSSIMPQKKNPDVPELARGKTGRVNGHLIALLTLMKGQPLAYNKDNQEDKEP
                     LFDTVDTLTDTLRIFADMARGITVNPQAMRAAALQGYATATDLADYLVRKGLPFRDAH
                     EAVAHAIRVCVDRNCDLSDLTLDELRAFSPLIENDIFGVLTLEGSIAARNHIGGTAPD
                     QVRLAIGEVRKSLQKQDHMPRG"
BASE COUNT         1039 a         1637 c         1409 g          935 t
ORIGIN      
        1 ctccagaatg gccgccagcg tatagcctgc aggcaaggcc atcggcatgt ccttgaggga
       61 atgcactgcg caatcggcgc gcccttcctc catggcgacc tcgagttcct tgacaaacag
      121 ccctttgccg ccgaccctgg acagggtacg atcgagaatc tggtcgccgc gcgtggtcat
      181 gccgaggata ctgatactgc actgcggata taatgccacc aagcggtcgc gcacatattc
      241 cgattgccac atggcgagac ggctttcgcg cgaagcgatg accagcttgg cgggaggaaa
      301 ttctttcaag acagttcttc cggtagccgc cgcgtcatcc gtgaagatga gcggcgcata
      361 aaaacataca agtgaggcca gcaaacgaac caaattctag cacgcagcac tattgacagt
      421 ttgtgctggc gcgcccggtt ttctcctttg cgaacagtgg caatagttga tcatggcaag
      481 taaatcacac cttccccccc tcgctcgcgc catcgccagc cttcccgaaa aacgtaccat
      541 caagtccgcc gacaaggatg ccccgctgaa agaagatatt cgcctgctcg gccgtctact
      601 cggcgaagtg ctgcgcgacc aggaaggtga tgctgtcttt gagatagtcg aaaccatacg
      661 tcagactgct gtgcgctttc gccgcgaatc cgacgcccgc gccggcgtcg aactcgacaa
      721 gctgctcagg aagctgacgc gcgaccagac caactcggta gtgcgcgcat tttcgtattt
      781 ctcacacctg gccaacattg ccgaagatca gcaccgtaac cgccgccacc gcgcccacct
      841 gctggcaggc tcgacaccgc agccgggtag tatagcctac gccctgtcac gcctgaacgc
      901 cgctggcgtc tccggcggcg ccgtgcgtga tttccttggt acagcgctga tttcgccggt
      961 gctgaccgct cacccgactg aagtgcagcg caaaagcatt cttgatgccg aatgcgaaat
     1021 cgcccgcctg ctgaccctgc gcgacaatcc attgaccccg aaagaattgc gcgacaatac
     1081 cgagctgctg cgtgcgcgta tcgccacact atggcaaacc cgcatgctgc gctttaccag
     1141 gctcaccgtt gccgacgaaa tcgacaatgc gctgtcttac tatcgcatca ccttcctgcg
     1201 cgcgctgccg gcgctgtatg acgacatcga agaagaaatc catacgcagt tctcctctca
     1261 cgccgcaccg gcagaactgc cgccgttcgt gcagatgggt agctggatcg gcggcgaccg
     1321 cgacggcaat cccaatgtca gcgccgacac catgcaacgc gcgctgcaac gccagtcggc
     1381 cacgatcttc gatttttatc tcgatgaagt ccacacgctc ggtgccgagc tgtcgatctc
     1441 gaccttcatg gtcgatgcca gcaccgcatt gctggcactg gccgatgcct cgcccgatac
     1501 ttccagccat cgtgccgatg agccttaccg gcgcgcgctg atcggcgtgt atgcgcgtct
     1561 ggcagcgacc gcccgcgcgc tcggcgtcgt caacatcctg cgccaggaag tcggcgccgc
     1621 cgcgccttat ggcagtccgg aagagtatat cggcgaactg caagtgctgg ttgactcgct
     1681 caaagctcac cacggcggcg cgctggtcaa gccgcgcctg gcggtgttga aacgttcggt
     1741 tgaaatcttc ggtttccacc tcgccacgct ggacatgcgc caaagctcgg atgtccacga
     1801 gcgcgtgctg gacgaactgt gcgccagcgc gcaagccgag ccgtcctacg ccgctttatc
     1861 cgagcaaaag aaaatcactc tgctgctggc cgagctgtcc aggccgcgcc tgctttactc
     1921 accctacctg cactattcgg acgaaaccat ctccgaactg gccaccctgc gcatggcgca
     1981 tgaaatccgc cgccgctacg gcgctcgcgc catccgcaac tacatcatct cgcataccga
     2041 aaccctgtcc gacctgctgg aagtcttgct gctacaaaaa gaaaccggtc tgctgcatgc
     2101 tgacgcgaac cggcagatga cggagctgga actgatggtt gtccctttgt tcgaaaccat
     2161 ccccgacctg cgccgcgccg ccggcatcat ggcgcagtgg ctggcactgc cgcaggtggc
     2221 gccgctgatc gcccggcaag gccgactgca ggaaatcatg ctcggttatt ccgattccaa
     2281 taaggacggc ggcttcctca cctccaactg ggagttatac aaatcggcaa accgtctgat
     2341 cgaactgttc aaccgcgctg acgtcaggtt acgtctgttc cacggccgcg gcggcaccgt
     2401 cggccgcggc ggcgggccaa gctatcaggc catcctggcg caaccgccag gcactgtcaa
     2461 cggccagatc cgcctgaccg agcagggcga aatcattgcc tccaaatttt ccaacccgga
     2521 aatcggtcgc cgcaatctgg aactgctggt cgcggcgacg ctggaagcca gcctcatgcc
     2581 gcatgccacc gataccaagg aagcacgcaa gctggccgaa tttgaacagg tcatggaaga
     2641 cctgtcggag cgggcctatc agacttaccg caatctggtc tatgaaaccc caggcttcac
     2701 cgattacttc ttcgccgcca cgccgatcgg cgagatcgcc gaactcaata ttggttcgcg
     2761 cccggctgcg cgcaaatcga tgcgccgcat cgaagatctg cgcgctatcc cctggggttt
     2821 ctcctggggt caatgtcgcc tgttgctccc gggctggtac ggtttcggcg gcgccatttc
     2881 cggctggctc caggaagtcg gcagcaaagg acgcgccaaa cggctggcca cgttgcgcgc
     2941 gatgtaccag gaatggccgt tctttgccgc gctgctgtcg aatatggaca tggtactgtc
     3001 caagactgac ctggcagtgg cctcacgtta tgccgggctg gtcagcgacc acgcactacg
     3061 cactgcgatt ttcggacgta tcgcggacga gcatgaacgc agcagtcgcc tgctcagcgc
     3121 catcagcggt aaccaggaac gcctggcaag caatccactg ctggcgcgct cgatcaagaa
     3181 ccgctttgcc tatctcgatc cgctcaatca cttgcaggtc gagctgatca gtcgtcgccg
     3241 cagtgcagtg gcggccggca ggcagatcga agaacgcgtg cagcgcggca ttcacctgac
     3301 catcaacggc attgccgccg gcctgcgcaa taccggctga tcgcggttac ggaatatact
     3361 gaactaacgg gcagggtcgc accgcgcgcg gcatgatgca catctcatcc gctataatcg
     3421 caaattctcc ggggctacat gatgaccaca caattatcaa aaaaaagcga agcctggtcg
     3481 gcgcgctttt ccgaacccgt ttcggaactg gtgaagcgtt atacagcttc cgtttttttc
     3541 gacaaacgcc tggcgccatt cgacatcgaa ggctcactcg cgcacgctga aatgctggcg
     3601 cagcagagca tcatcagcgc ccgggattac gccgatatcc agcgcggcat ggcgcagatc
     3661 aagagcgaaa tcgacgatgg ccggttcgcc tggctacccg atctcgaaga cgtccatctg
     3721 aatatcgaaa aacgcttgac agaactggtc ggcgacgccg gcaaacgcct gcataccggc
     3781 cgctcgcgca acgaccaggt cgcaacggac atccgcctgt acctgcgcgc cgctatcgat
     3841 ggcatcgacg gcctgctgcg cgagctgcgc acagcgctgg ttgatctggc cgaacagcat
     3901 gccgacacca tcatgcccgg cttcacacac atgcaggtag cgcagccggt caccttcggc
     3961 caccacatcc tcgcttacgt cgaaatgttc agccgcgacg ccgagcgcat ggctgacgcc
     4021 cgcagacgta tcaaccgcct gccgctgggt gccgctgcac tggccggcac gaccttcccg
     4081 atcgaccgtc tgcgcgtagc caggacgctg ggcttcgacg atgtctgccg caactcgctg
     4141 gacgccgtct ccgaccgcga cttcgcaatc gaattttgcg ccgccgccgc gctggtcatg
     4201 acccatatct cgcgcatgtc cgaagaactg gtgatctgga tgaacccgcg cctagctttc
     4261 atcgatatcg ccgaccgctt ttgtaccggc tcatcgatca tgccgcaaaa gaaaaacccg
     4321 gacgttcccg aactggcacg cggcaagacc ggtcgtgtca atggtcatct gatcgctctg
     4381 ctgaccctga tgaagggcca gccgttggcc tacaacaagg ataatcagga agacaaggag
     4441 ccgctgttcg ataccgtcga tacgctcact gacacactgc gcattttcgc cgacatggcg
     4501 cgcggtatca ccgtcaatcc gcaggccatg cgcgccgctg cgttgcaagg ctatgctacc
     4561 gccaccgacc tggctgacta tctggtcaga aaggggctgc cgttccgcga tgcccatgaa
     4621 gccgtcgctc acgccatacg tgtctgcgtc gaccgcaact gtgatctgtc ggacctgacg
     4681 ctggacgaac tgcgcgcgtt ttcgccgctg atcgaaaacg acatcttcgg cgtcctgacg
     4741 ctggaaggct ccatcgccgc gcgcaaccac atcggcggca cggcgccgga tcaggtgcgg
     4801 ttggcgatag gagaagtgcg caagtcattg cagaaacaag accatatgcc cagaggatga
     4861 acaaggcaca atagcgccgc gagggatcgg cggcggcacg cgggtggatc cgggcgcagg
     4921 gggaggggta attggcggag acggagggat tcgaaccctc gatacaggtt taagcccata
     4981 tgcttcctta gcagggaagt gccttcgacc actcggccac
//
LOCUS       DASRDH010000224         6081 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_332243,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000224 DASRDH010000000
VERSION     DASRDH010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 6081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6081
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332243"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2076
                     /locus_tag="VFA14_05155"
     CDS             <1..2076
                     /locus_tag="VFA14_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HZS80796.1"
                     /translation="TGFSRMLGFAAGEVIGKQPRELFEKQPAALKLIDDYHAEITQGN
                     SFHGEGMMLDKQGHPLWVSMVVNPIHDSSSKLVNTVCILTDITNTKMHEVLQYKALDA
                     MVREASLQEVLTMVCLELERIAPNVVASVLSVDENGLLHPVAGPHLPEQYNRAIDNTP
                     IGPNVGSCGTAAFRDEAVVCDIESDPLWADYKHLVLPLGFLTCWSTLIKSADGRVIGT
                     LAFYYRDKRKNDAFHHRLIEASLHLCALALEREETHSRIHQLAFYDSLTGLPNRRLLM
                     VKANQAIANAARTLTPLAVLFIDLDRFKQINDSLGHQAGDELLRTVAARLQFEARKSD
                     IVGRLSGDEFVVVLTQCDLYHITDVIKRIQTNLSLPCQIAGVTLTPLASIGVSLFPDN
                     GNTIEILLHRADMAMYQTKGKNRGRFSFFSDDMNTQAQERLTLETALRDALRDGDFEL
                     YYQPQINIKNSLLHGVEALARWHHAQLGMVSPARFIPIAEECGLIGELGKWALEEACC
                     QLSDWRKRGIDIPSVSVNLSSTNFHDLDLPRFIADLLLHYALTPGDLILEITETVILN
                     ANPSTTHTIHEIHAQGVRLAMDDFGTGYSSLGYLRNLPVSELKLDQSFVRDMNRDETV
                     LALTHAVIRIGESLDLTVVAEGVENRQQYKLLKQQGCDAVQGHLVSPPMPASAFERWL
                     KDFDASDIF"
     gene            2228..3223
                     /locus_tag="VFA14_05160"
     CDS             2228..3223
                     /locus_tag="VFA14_05160"
                     /inference="COORDINATES: protein motif:HMM:NF020452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1837 domain-containing protein"
                     /protein_id="HZS80797.1"
                     /translation="MTSGDNVQSETASQPEGETPKKSLEEALVRLARGEPTALAGLLH
                     DVESYALVNGTKVTTHCHCLKLDGNGRPRIEDLVKVVAEHVLDYAIPRTQIREAIDEV
                     SRTGSTQKIVRLENEAKALFTDLERSGEGGELLLFALAEKILRLPQIICKMSLKTNTR
                     MHVHGADGLHAGADSTTGKLLLYWGESKIYGNVTNAIRECLASVAPMLTKNSAGQRDL
                     QLLQRHADLNDPALETAFKKFLDPDSEEFNSLEFRGLCLVGFDCDAYPGASSATQVST
                     IVQEIAKILPIWKEHVKKQISKEKLDVFAMHFLLVPFPSVEKFRKFIRMSLVGKK"
     gene            3223..5853
                     /locus_tag="VFA14_05165"
     CDS             3223..5853
                     /locus_tag="VFA14_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006220084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZS80798.1"
                     /translation="MALRDLGDWLLNVPGFRHQESKVVIDAACDELGSQFHRRKEHQG
                     IQHDWDYLLFAASFLAQSNKGAAIALRVAQTCLCDKMATDVQRDSAALILDALANHPA
                     IELAVKRSLLRPSFQTRLPGVARLDYSRRTYEQSISIHGDKPLRVNGFQKKLWEAARN
                     HGWISVSAPTSAGKSFILARWICNVMRSFPVTTIVYLVPTRALISQVERDLRDLFSKE
                     ELDKVSVSALPIVKPEDGTDVIRQRVLVFTQERLHILFSAQPNLAVQALIVDEAHKVG
                     DPQRGVLLQDVIERLSIANPNLSVLFASPMTSNPELLLADARPGTWTTSVTSEDVTVT
                     QNLFWVSQRSQKPKVWDIKLCMHDESIELGEVELSSKPSSDSKRLSFIAHAIGGSSHG
                     NVVYVNGAADAEKIAGQIFDLIGTDIEGESGKALHSLIELAQHGVHKEFALATCLRRG
                     VAFHYGNMPLLVREEIEHLFSTGIIKYLVCTSTLIEGVNTACRNVFMRGPTKGRSRPL
                     SAEDFWNLAGRAGRWGKEFQGNIFCIDPDRRNLWGEDGPPRKRTRHTIRRTTDDVLAT
                     SLDLIDYIRERAPLEKAKKHPEFEHVFSYLAGIHVRHGGVLEAPWAKRYGASSLKQLA
                     EAVKQAVDELAVEPDTLAQNPGINPFALDDLLKDFRERQGDVEELLPVDPTSNDAAKV
                     YADIFGRLCRRACPNLGPEGGRAFMLALVVTHWMRGFPLARLIEERLKYLRKKQKPTD
                     IATEIRNVMNEVEKIARFEAPRGLSCYCDVLRQHLIEIKREDLIGQLPQINVFLELGV
                     SQQTQISLIGIGLSRTSAIAVSDFITPDSLTEHQVLQWLVENEKIWQNFSLPILVKKE
                     IGRVLGQHGEMKKRSSGQKV"
     gene            5861..>6081
                     /locus_tag="VFA14_05170"
     CDS             5861..>6081
                     /locus_tag="VFA14_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZS80799.1"
                     /translation="MNAVEHPSRPQRPIQALPDQLISQIAAGEVIERPSAVIKELLEN
                     ALDAGATHITIRLEQGGIKRIAIIDNGCG"
BASE COUNT         1525 a         1628 c         1586 g         1342 t
ORIGIN      
        1 accggcttta gccgcatgct tgggtttgcc gccggtgaag tcatcggcaa acagccacgc
       61 gagctgtttg aaaaacagcc agcagccctg aaactgattg acgattacca cgccgagatt
      121 acacaaggca acagcttcca cggcgaaggg atgatgctcg ataaacaggg acatccattg
      181 tgggtatcga tggtggtcaa tccgattcac gacagcagca gcaagttggt gaatacggtc
      241 tgcatcttga ctgacatcac caataccaag atgcatgaag tgctgcaata caaggctctc
      301 gacgcgatgg tgcgtgaggc cagcctgcag gaagtactga ccatggtgtg cctggagctg
      361 gaacgcatcg caccgaatgt ggtcgcttcg gtcctgagcg tcgacgaaaa tgggctgctg
      421 cacccggttg ccgggccgca cctgccggaa caatacaacc gtgccatcga caacacaccg
      481 atcggcccga acgtaggctc ctgcggcacg gcagcctttc gcgacgaggc agtggtctgc
      541 gacatcgaga gcgatccgct atgggctgat tacaaacatc tggtgctgcc gttaggattc
      601 ctgacatgct ggtcgacgct gatcaaatcg gccgacggac gcgtcatcgg cacactggct
      661 ttttattacc gcgacaaacg caagaacgat gccttccatc atcggctgat agaagccagc
      721 ctacacctct gcgccctggc gctggagcgc gaagaaaccc actcgcgcat ccaccaactg
      781 gccttctacg attcgctgac cggcctgccc aatcgccgcc tgctgatggt caaagccaat
      841 caggccatcg ccaatgccgc acgtaccctg actccgctgg cagtgctgtt catcgacctc
      901 gatcgcttca aacagatcaa cgactcactc ggccaccagg ctggcgacga attgctgcgc
      961 acagtcgccg cgcgcctgca attcgaggca cgcaaatcgg acatcgtcgg ccgcctgtcc
     1021 ggtgacgaat tcgtcgtcgt gctgacacag tgcgatctgt atcacatcac cgacgtcatc
     1081 aagcggattc agaccaacct gtcgctaccc tgccaaatcg ccggcgtaac actgacgccg
     1141 ttggccagca tcggcgtcag cctgttcccc gacaacggta ataccatcga aatcctgctg
     1201 catcgcgctg acatggcaat gtatcaaacc aagggaaaaa atcgcggccg cttcagtttt
     1261 ttcagcgatg acatgaatac ccaggcgcaa gagcgtctga cgctggaaac tgccctgcgc
     1321 gatgccctgc gcgacggcga tttcgagttg tactatcaac cgcagatcaa tatcaaaaac
     1381 agcttgctgc acggcgtgga agccttggca cgctggcatc acgcgcagct gggcatggtg
     1441 tcaccggcgc gcttcatacc gattgcggaa gaatgcggac tgatcggcga actgggaaaa
     1501 tgggcgctgg aagaagcctg ttgtcaactc agcgactggc gcaagcgtgg catcgacatt
     1561 ccctcagtgt cggtcaatct gtcgtcgacc aattttcacg atctcgatct gccgcgattc
     1621 atcgccgact tgctgctgca ttacgcgctg accccgggcg atctgatact ggaaatcacc
     1681 gaaaccgtga tactgaacgc caaccccagc accacgcaca ccattcacga aattcacgcg
     1741 caaggcgtca gactggccat ggatgacttc ggcaccggtt attccagtct cggctatctg
     1801 cgcaatctac cggtatccga gctgaaactc gatcaaagtt tcgtgcgcga catgaatcgg
     1861 gatgagaccg tgctagcgct cacgcatgca gtcatccgta tcggtgaaag cctcgacctg
     1921 accgtcgtcg ccgaaggcgt ggaaaacaga cagcaataca agctgctcaa acaacaggga
     1981 tgcgatgcag tccagggtca tctggtttca ccgccgatgc cggccagcgc tttcgagcgc
     2041 tggctgaaag atttcgacgc cagcgatatt ttctgaaaac tgtaccagaa aaaattgacg
     2101 gcgaccggga gcagttccgc ctcaccgctc gaactggtaa gcacttaaaa taggttagat
     2161 ttattatttt gtccgccatg ggcaaccaaa aattattaat aatccgctac atatgtggag
     2221 gcaaataatg acaagtggcg ataacgtaca gtcggaaact gcaagccaac cggaagggga
     2281 aacaccgaaa aaatcgcttg aggaagctct ggtgcgcctt gcccgaggcg agccaaccgc
     2341 acttgcggga ctgcttcacg atgtcgagag ttatgccctt gtaaatggca caaaggtcac
     2401 aacgcactgt cactgcctca aactcgatgg gaacgggcgc cctcgaattg aagatttggt
     2461 gaaggttgtc gctgagcatg tcctcgacta cgcaatccca agaacccaga tacgcgaagc
     2521 catcgatgaa gtctcgcgca caggttctac gcagaagatc gttcgcctag aaaatgaggc
     2581 caaagctctg ttcacggatc ttgagcggag tggagaggga ggggaattgc tactttttgc
     2641 tctcgcagaa aaaatccttc gtttacccca gatcatctgc aaaatgagcc tgaaaaccaa
     2701 cacgagaatg catgtccacg gtgcagatgg actgcatgca ggcgccgatt cgactactgg
     2761 caaacttctc ctttactggg gcgaatcgaa aatctacggt aacgtcacca acgccattcg
     2821 cgagtgcttg gcatctgttg caccaatgct cacaaaaaac tcggctgggc agcgcgacct
     2881 tcagttactc cagcgacatg cagatctgaa tgatcctgcg ctggagacgg ccttcaagaa
     2941 atttcttgat ccagactcag aagaattcaa ctcacttgaa ttccgaggac tgtgccttgt
     3001 cgggtttgac tgtgatgctt accctggtgc ctcatcagcc acccaggtca gtactatcgt
     3061 ccaagagatc gcaaaaatac ttccaatctg gaaagagcat gtcaaaaaac aaatttccaa
     3121 ggaaaagcta gatgtattcg caatgcactt tctacttgtg ccttttccat cagtagaaaa
     3181 atttagaaaa tttatcagaa tgagtttggt cgggaagaaa taatggcgct acgtgatctt
     3241 ggagactggc tgctcaatgt tcctggattt cgccatcagg aaagtaaggt tgtaattgat
     3301 gcagcgtgcg acgaattggg ctcgcaattt catcggcgta aagaacatca gggaattcaa
     3361 catgactggg actatctact gttcgcagca agctttctcg cgcagtccaa caagggggct
     3421 gccattgcgc ttcgagtcgc ccaaacgtgt ctttgcgata agatggcgac ggatgtgcag
     3481 cgtgactctg ctgcactgat tcttgatgcg ttggctaatc acccggcaat cgagcttgct
     3541 gtcaagcgga gccttcttcg tccatcgttt caaacgcgct tacctggcgt tgctcgcctt
     3601 gactattcac gccgtaccta cgagcaatcg atctcgatcc acggagataa acctctccga
     3661 gtgaacgggt ttcagaaaaa actctgggaa gctgcgcgca atcatgggtg gatatcggta
     3721 tcagcaccaa catctgcggg caagtcgttc attttggctc gttggatatg taatgtgatg
     3781 cgttcatttc cagttacgac gatcgtttac ctcgttccga ctcgcgcact catcagtcag
     3841 gttgaacgag atcttcgtga cctgttcagt aaggaggagc ttgataaggt atcggtatcg
     3901 gcattaccaa tcgtgaaacc tgaagatggc acagacgtga ttcgtcagcg ggttcttgtt
     3961 tttacacagg aacgattaca catcctcttt tcggcgcaac ccaacttggc agtgcaagca
     4021 cttatcgttg acgaagccca caaggtcgga gaccctcagc gcggcgtgct gctgcaagac
     4081 gtcatagagc gcttgagtat agccaatccg aatctgagtg tgctcttcgc aagcccaatg
     4141 acaagcaatc ctgaattgct actcgcagat gcgcgtccag gaacctggac aacatctgtc
     4201 accagcgaag acgtgaccgt aacccaaaat cttttctggg tgtcgcaacg ctcacaaaaa
     4261 cccaaggttt gggatatcaa gctttgcatg catgacgaga gcatcgagct tggggaagtc
     4321 gaacttagtt ccaaacccag ttctgatagt aagcgcttgt catttattgc tcatgctatt
     4381 ggaggttcct cgcacggcaa tgttgtttat gtgaacggtg cagcggatgc tgaaaaaatt
     4441 gctgggcaaa tatttgatct gatcggcaca gatatcgagg gcgaatcggg gaaagccctg
     4501 cattccctta ttgaacttgc acagcatggt gttcacaaag agttcgctct tgcaacctgc
     4561 ctgcggcgcg gagtggcctt tcactacggg aatatgccgc tgctagtcag agaagagatt
     4621 gaacaccttt ttagtactgg catcatcaaa tatctcgtat gcacatcaac gctcatcgaa
     4681 ggcgtcaata cagcttgccg taacgtattc atgcgagggc cgaccaaagg ccgtagtcgc
     4741 ccgctttcag ccgaggattt ttggaacctt gcaggccgcg ccgggcgttg gggtaaagag
     4801 tttcaaggca atatattttg tatcgaccct gatcgaagga atctttgggg agaggatggg
     4861 ccgcctcgta aacgaacgcg ccacacgatc cggcgcacga ctgacgatgt tctagccacc
     4921 tctttggatt tgattgatta catcagggag cgtgctccgc tcgaaaaagc gaagaaacat
     4981 cccgagtttg agcatgtctt ctcgtatttg gccggaattc acgttcgcca tggtggagtc
     5041 cttgaagcgc cttgggcgaa gcgctatggc gcgagcagtc tgaagcaact tgcggaggca
     5101 gtaaaacaag ctgtcgatga acttgccgtc gaaccggaca ccctggccca gaaccccggc
     5161 ataaatccct ttgcgcttga cgatctcctc aaggatttcc gggaaaggca gggcgatgtt
     5221 gaagaattgc tacctgtaga ccccacgagt aatgacgcag ccaaagtgta tgctgacatt
     5281 ttcggacgtc tctgccgacg cgcgtgcccc aatcttggtc ccgaaggagg tagggccttc
     5341 atgctggctt tagtagtcac gcactggatg cgtggtttcc cgctcgcacg actaatcgag
     5401 gagcgactca aatacctcag aaagaagcag aagcccacgg atattgctac agagatcagg
     5461 aatgtgatga atgaagttga gaaaattgcg cgctttgaag ccccgcgggg tctgagttgc
     5521 tattgcgacg tgcttcgaca acatttgatc gagatcaagc gtgaagatct gattgggcaa
     5581 ctcccacaga tcaatgtatt tcttgaattg ggagtgagcc agcagacaca gatttcgctg
     5641 atcggcattg gtctctctag aacctcagct atcgcggtgt ctgacttcat cactccagac
     5701 tccttgacag agcatcaggt gcttcaatgg ctcgtcgaaa atgagaaaat atggcagaat
     5761 ttttcgctac caatactggt gaagaaagaa atcgggcgag tccttggtca gcatggtgaa
     5821 atgaaaaaac gctcatccgg ccagaaggta taatcgtcag atgaacgccg tagaacaccc
     5881 cagccgtcca cagcggccca ttcaggcgct gcctgaccag ttgatctcgc agatcgctgc
     5941 gggtgaggtc atcgagcgcc cctctgccgt gatcaaggaa ttgcttgaaa acgcgctcga
     6001 tgccggcgcc acgcacatta ccatacgtct agaacagggc ggcatcaaac gcattgccat
     6061 catcgacaat ggttgcggca t
//
LOCUS       DASRDH010000225         3227 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1645038,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000225 DASRDH010000000
VERSION     DASRDH010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 3227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3227
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1645038"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="VFA14_05175"
     CDS             <1..280
                     /locus_tag="VFA14_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HZS80800.1"
                     /translation="LAFGITFEVPIVVIVLVRMGLVPLARLRQIRPYIVVGAFIIAAV
                     VTPPDVMSQLMLAVPLCLLYEVGLVIAPIFERAARAPEEQDDPVGNGQ"
     gene            complement(297..1466)
                     /locus_tag="VFA14_05180"
     CDS             complement(297..1466)
                     /locus_tag="VFA14_05180"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007879652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HZS80801.1"
                     /translation="MQRFWLLFAQTVTVGLAVWFIVVTLKPDWAGGPFNSRVRSVVSL
                     VQMQEAAPGALTPNSYRDAARKAMPSVVNVFTTTEARPQRNPFMDDPFFRKFFGDQFE
                     NQQDDKQSSLGSGVIVSPQGYILTNNHVVEAADEIEVALADGRKASAKVVGTDPETDL
                     AVIKIDLPNLPTITLAHVEQATVGDIVLAIGNPFGVGQTVTMGIISALGRNHLGINTF
                     ENFIQTDAAINPGNSGGALVDTSGNLLGINTAIYSRTGGNLGIGFAIPMSTAKNVMEA
                     IINHGQVVRGWIGVEPQDITPELAESFSLTRKAGTIIAGVLKNSPADKAGMKPGDILI
                     SIEGKPVADTTDMLNLIAQLTPGQKAKMLVLRKAQQTTLDIVIGKRPPLKREDRE"
     gene            1494..2264
                     /locus_tag="VFA14_05185"
     CDS             1494..2264
                     /locus_tag="VFA14_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HZS80802.1"
                     /translation="MTLCIADRDELGNYLAQVLNITQYRDYCPNGLQVEGRAEIGLVV
                     SGVTASQALIDAALDLRADAILVHHGYFWRGEEARVTGTRRKRLKALLSNDLNLFAYH
                     LPLDAHPEWGNNAQLARRLNLVGKDHFGENGLGWSGMPQTDAGIAIVGDLVDLIAHRL
                     GRMPLLIGDADQPVGKVAWCTGGAQGFLGDAIAAGASVYISGEISEPTVHLAREAGVA
                     YIAAGHHATERYGVQALGAHIADRFGIRHQFVDIDNPV"
     gene            2401..3009
                     /gene="petA"
                     /locus_tag="VFA14_05190"
     CDS             2401..3009
                     /gene="petA"
                     /locus_tag="VFA14_05190"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006461588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HZS80803.1"
                     /translation="MNDEKQVDASRRGLLVATCAAGGVAGLATAGAFVSTFQPSERAK
                     AAGAPVEVDISTLAPGEMKTVEWRGKPVWILRRTPEMVGSLKKVDTQVADPQSRRTEF
                     STTPEYAFNEWRSIRKDVLVVVGICPHLGCSPSTKLQAGVQPSLPDDWQGGFLCPCHG
                     STFDLAGRVYKNKPSPDNLEVPRYMFEGDTRLVIGRDEKGEA"
     gene            3012..>3227
                     /locus_tag="VFA14_05195"
     CDS             3012..>3227
                     /locus_tag="VFA14_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008332421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HZS80804.1"
                     /translation="MAVFEERQMPQDAPVSAKVLNWVDARFPLTSTWKAHLSEYYAPK
                     NFNFWYAFGSLALLVLVIQIVTGIFLVM"
BASE COUNT          646 a          870 c          969 g          742 t
ORIGIN      
        1 tctggcgttt ggtatcacct ttgaagtacc catcgtcgtg atcgtgctgg tgcgcatggg
       61 gctggtgccg ctggcgaggc tcaggcagat ccgcccttat atcgtggtcg gcgctttcat
      121 cattgctgca gtcgtcacac caccggatgt catgagccag ctcatgctgg ctgtgccgct
      181 gtgcttgctg tatgaagtcg gtctggtgat tgcgccgatt ttcgagcgtg ccgcgcgcgc
      241 accggaagag caggacgatc ctgttggcaa cgggcagtaa aagaatagcg cctgctttat
      301 tcgcggtcct cgcgttttaa cggtggacgt ttaccaatga cgatgtccag tgtggtttgc
      361 tgtgctttgc gcagcacgag catcttggct ttctggccgg gtgtcagctg tgcgatcagg
      421 ttcagcatgt cggtggtgtc ggccaccggt ttaccctcga tgctgatcag gatgtcgcct
      481 ggcttcatac ctgccttgtc ggcagggctg ttcttgagca cgccggcaat gatggtgcca
      541 gctttcctgg tcaggctgaa gctttctgct agttcaggcg tgatgtcctg cggttcgacg
      601 ccaatccagc cgcgcacgac ctgaccgtgg ttgatgatag cttccatcac gttttttgcg
      661 gtcgacatcg ggatcgcgaa accgatgcca aggttgccgc cggtacgcga atagatcgct
      721 gtattgatgc ctagcagatt gccgctggtg tcgaccagcg cgccgcctga gttgcccgga
      781 ttgatcgctg catcggtctg gatgaaattt tcgaaggtat tgatgccgag gtgattgcgg
      841 cctagcgccg agataatgcc catggtgacg gtctggccga cgccgaaagg attgccgatg
      901 gccagcacga tgtcaccgac ggtggcttgt tcgacatgcg ccaacgtgat ggtaggcagg
      961 ttcggcaaat cgattttgat cacggcaagg tcggtctcgg ggtcggtgcc gactaccttg
     1021 gcagaggcct tgcgaccatc agccaatgcc acttcgattt catcggcggc ttcgaccaca
     1081 tgattattgg tgaggatata gccttgcggg ctgacgatga cgcctgaacc gaggctggat
     1141 tgtttgtcat cttgttgatt ttcgaattga tcaccgaaaa atttcctgaa aaacggatca
     1201 tccatgaatg gatttctttg cggacgcgct tcggtcgtgg tgaagacgtt gaccaccgat
     1261 ggcatggcct tgcgggcagc atcacgatag gagttcggcg tcagcgcgcc gggagcggcc
     1321 tcctgcattt gtaccaacga tacgacagag cgcacgcggg aattgaaagg cccgccggcc
     1381 cagtccggct tcaaggtgac tacaataaac cagaccgcca aaccgacagt cacggtttgc
     1441 gcaaacaaca gccaaaaacg ttgcatatat tgaaatgaaa atgaggatga aaaatgaccc
     1501 tgtgcatagc agatagggac gagctgggca attacctggc acaagtgctg aatatcacac
     1561 aataccgcga ttattgccca aatggcttgc aggtggaagg gcgtgccgag attggcctag
     1621 tggtgagcgg ggtaactgcc agccaggcgc tgatcgacgc cgccctggac ttacgggccg
     1681 acgccattct ggttcatcac ggctacttct ggcgtggcga ggaggcgcgc gtcactggca
     1741 ccaggcgcaa gcgcctcaag gccttgttga gcaacgatct caacctgttt gcttatcatt
     1801 tgccgctgga cgctcatcct gaatggggca acaatgcgca attggcgcgt cgcttgaatc
     1861 tggtcggtaa ggaccatttc ggcgaaaacg gactgggctg gtcaggcatg ccgcagaccg
     1921 acgccggcat tgccatcgtc ggtgaccttg tcgatctgat cgcgcatcga ctggggcgca
     1981 tgccgttgtt gatcggcgat gcggatcagc cggttggcaa agtggcctgg tgtactggcg
     2041 gcgcgcaagg ttttcttggc gatgcgatcg ccgccggcgc ctcggtctac atcagcgggg
     2101 aaatttccga accgactgtg catctggcgc gtgaggccgg cgtggcctat atcgccgccg
     2161 gccaccatgc cactgagcgt tacggcgtgc aggcgctggg cgcgcatatc gccgatcgtt
     2221 tcggcatccg ccatcagttc gtcgatatcg acaatccggt ctgagatctt gaacaggttc
     2281 tttgcatcgt gcgcattttt tcttctggaa attgttgcaa aacgatgcag tcggttatat
     2341 cgtgctccgg gaaagtcaat tgtggtcaat gagtttcaca atacaaaaac tggggttggt
     2401 atgaatgacg aaaaacaggt cgatgccagt cggcgcggac tactcgtcgc gacttgcgcg
     2461 gcgggtggtg tggccggcct ggctaccgca ggagcttttg tttccacctt tcagccttcc
     2521 gaacgcgcca aagccgccgg cgctccggtc gaagtcgata tctccacgct tgcgccaggc
     2581 gaaatgaaaa ccgtcgaatg gcgtggcaaa ccagtctgga ttctcaggcg tactcccgaa
     2641 atggtgggct cgctcaaaaa agtcgataca caagttgccg atccgcaatc cagacgcacc
     2701 gaattttcga ccactcccga atacgcattc aacgaatggc gttcgatcag gaaagatgtc
     2761 ctggttgtgg tcggcatctg tccacacctc ggctgttcgc ccagcaccaa gttgcaggcc
     2821 ggcgtccagc cctcgctgcc ggatgactgg cagggtggtt ttctgtgccc gtgccacggt
     2881 tcgacgttcg acctggccgg ccgggtctac aaaaacaaac catctccgga caatctcgag
     2941 gtgccacggt atatgtttga aggcgacacc agactggtca tcggcaggga cgagaaaggc
     3001 gaggcgtaat catggctgta ttcgaagaaa ggcagatgcc ccaggatgcg ccggtatcgg
     3061 ccaaagtgct gaactgggtc gatgcccgtt ttccgttgac ctccacctgg aaagcccatc
     3121 tttccgaata ttacgcccca aaaaatttca atttctggta cgccttcggg tcgctggcgc
     3181 tgctggtgct ggtcatccag atcgtgaccg ggattttcct ggtgatg
//
LOCUS       DASRDH010000226         8820 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_880418,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000226 DASRDH010000000
VERSION     DASRDH010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 8820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8820
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_880418"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="VFA14_05200"
     CDS             <1..274
                     /locus_tag="VFA14_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HZS80805.1"
                     /translation="ARLPLGDVRQHSPAVMLNILGDVWYEGDSGAMREPAWDKILALP
                     GAHLHLYGKAQARRARKMGHITFVAPTMAQVQVQLAAACAILGITL"
     gene            285..1307
                     /locus_tag="VFA14_05205"
     CDS             285..1307
                     /locus_tag="VFA14_05205"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013232713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HZS80806.1"
                     /translation="MIAVDMDAVRLAARKLEAGRLVAFPTETVYGLGGDAGNAVAINA
                     IYAAKGRPANHPVIVHIAPEADISDWASVVPAQAHALTAAFWPGPLTLVLKRAAHISA
                     AVSGGQDSIGLRCPSHPVAQALLREFQGGRGGLAAPSANKFGHVSPTTAAHVREEFGN
                     DAGGLVDYVLDGGQSEVGIESTILDLSRVTTHGAVLLRPGQISIDQIAAVLGVTPMAP
                     DAAAPRASGTLDGHYAPHTPVAQVPAQELPQLMQALADRHCRFAVIHRTVAVPSLSAS
                     FAMSHDAGYYAHDLYAALREMDQCGADIILVEALPHMSEWQGINDRLRRAAFDSTGIL
                     ARLLAA"
     gene            1365..1805
                     /locus_tag="VFA14_05210"
     CDS             1365..1805
                     /locus_tag="VFA14_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007880219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HZS80807.1"
                     /translation="MRRSLALLTVLAAFSAALPADAQQLQKLPTITLNVGLYLIEAEV
                     ASTEAQRNRGLMFRNSMGANEGMVFVYGAPAEVCMWMKNTAIPLSVAFMDAAGHIINI
                     EEMKEQTLDSHCATRAASYALEMNKGWFRQKNIKPGMMIQGLPR"
     gene            complement(1857..2657)
                     /locus_tag="VFA14_05215"
     CDS             complement(1857..2657)
                     /locus_tag="VFA14_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014749183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HZS80808.1"
                     /translation="MDTQTRHEMHGRWPQAGSAEDFRRNLADVHGRIAAACRRVGRDP
                     ESVRLLPVSKSRPEASIRLSYAAGCRLLGENKPQEAYRKWEATTDLNDLHWSVIGHLQ
                     TNKARLVARCASEFQALDSLRVAEALERRLQLEGRSLDVFVQVNTSGEASKYGLHPDE
                     VAVFLRALPSFPALRVRGLMTLALLSTDPVRVRQCFVLLRSLRDRLRQEAPEGIALNE
                     LSMGMSGDYEIAIEEGATVVRVGQAIFGARATPDSHYWPTSGDVEGRQ"
     gene            complement(2763..3029)
                     /gene="rpsT"
                     /locus_tag="VFA14_05220"
     CDS             complement(2763..3029)
                     /gene="rpsT"
                     /locus_tag="VFA14_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HZS80809.1"
                     /translation="MANTAQARKRVRQAVKQNAHNSSQRSMLRTAIKAVRKAIETGDK
                     IAATSVFRTSVSTIDSIADKKIIHKNKAARHKSRLAAAIKALAA"
     gene            3220..4770
                     /gene="murJ"
                     /locus_tag="VFA14_05225"
     CDS             3220..4770
                     /gene="murJ"
                     /locus_tag="VFA14_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007880216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HZS80810.1"
                     /translation="MNLHKTLVAISGMTMLSRITGLAREILFARAFGASSYTDAFNVA
                     FRIPNLLRRLFAEGAFSQAFVPILAEYKNQRGNAATRHLVDHVATVLIWTMLATSIIG
                     ILLAPVMVYFIATGLKYDQGAFDASVVMTRIMFPYIGFMAFVALAGGILNSWREFRIP
                     AFTPVLLNLSFIVAALCVAPYLDQPIYAMAFAVFVGGMLQVLLQIPALIKVGMLPRLY
                     VNPAISLADEGVKRVLKKMGPAVFAVSAAQISLMINTNIASRLTHGSVSWLSYADRLM
                     EFPTALLGVALGTILLPSLSNAHAENDMAEYSALLDWGLRLSFLLAMPCAVGMATLAD
                     PLTATLFHYGQFDTLSVKMTGHALIAYGVGLTGLILVKILAPGFYARQYIWTPVKIAI
                     GVLIATQLMNCFFVPWMAHAGLALSIGLGACLNAALLFRGLRHRHLYTPQPGWRLFLI
                     KLTGALFLLGGVALTVARHFDWIAMRAHPAERIGALLLVLVACGVTYFGALMAMGFRY
                     DDFKRIRL"
     gene            4991..5260
                     /locus_tag="VFA14_05230"
     CDS             4991..5260
                     /locus_tag="VFA14_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS80811.1"
                     /translation="MNNDQNRGAMNWTDLEKHLNRVFHHEPLLWLTPALIDQHTLGLQ
                     AIGWLDPQLIDWLNHHDRLPVAWQSGRTFRPYSSDQAAPAIGVKP"
     gene            complement(5241..5585)
                     /locus_tag="VFA14_05235"
     CDS             complement(5241..5585)
                     /locus_tag="VFA14_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS80812.1"
                     /translation="MYDNEIKEAANEYPTGTAIVEKVYGDAGLYKVYVQTLDHPHVLE
                     MIVSASTKEAATQKALQHVKEGNPTKGRSLEESQKNSVVIAAKPAGKSGCIVTNKIPV
                     LAFEEIAKALRR"
     gene            5720..6559
                     /locus_tag="VFA14_05240"
     CDS             5720..6559
                     /locus_tag="VFA14_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007880215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZS80813.1"
                     /translation="MALTHLEYFSCLVRQDSSIPLFESALALGQDAYPDLDFSACQME
                     MDNLALKLRQRLLSDATHIQKLRVLNRFFFQELAFAGNANNFYDPDNSYIHRVMMSRR
                     GIPISLAVVYMELGQQIGLDIKGIPFPGHFLMKLSVQSGEIVLDPMNGASLSREELEV
                     RLGLYLERQGKSGELPLAVCLRAAHPREILTRMLRNLKVIFAEAQRWEHVLAVQKRLV
                     ILLPNEITERRDRGLAYANLAEPQAALKDLEAYLALRPDADDADIMRQRLPELRHYSR
                     RPG"
     gene            6702..7736
                     /gene="pyrC"
                     /locus_tag="VFA14_05245"
     CDS             6702..7736
                     /gene="pyrC"
                     /locus_tag="VFA14_05245"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HZS80814.1"
                     /translation="MRDVINMTRPDDWHLHVRDGATLASVLPHTAHQFARAIIMPNLK
                     PPVTTVALASAYRDRILAALPAGSSFEPLMALYLTDDTPPEEIRKARDSGFVHAVKMY
                     PSGATTNSDAGVTDLRKCGKTLEALQEVGMPLLIHGEVTDPAIDIFDREAVFIERVMM
                     PLRKDMPELKIVFEHITTRDAADYVMAAEGPTAATITAHHLLYNRNEIFKGGIRPHYY
                     CLPILKREMHRQALIAAAVSGSSRFFLGTDSAPHARGLKEHACGCAGCYTALHALELY
                     AQAFDQAGALDRLEAFASFNGPAFYNLPCNQGSVTLRRERWTIPTELPMGESMMVPLN
                     GGETMHWKMM"
     gene            7785..8411
                     /gene="coq7"
                     /locus_tag="VFA14_05250"
     CDS             7785..8411
                     /gene="coq7"
                     /locus_tag="VFA14_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008331904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone
                     monooxygenase"
                     /protein_id="HZS80815.1"
                     /translation="MQFIDQLITDLDKALRSVSGVTVSSRSNPAMHITDAAMSDEEKR
                     HSAGLMRVNHVGEVCAQALYDAQGRFAQTQALKQLFAHAGLEEEDHLAWTAERLRELG
                     SHTSLLNPLWYAGSYVLGAIAAHMGDARSLGFVVETERQVEQHLTSHLGKLPLQDARS
                     RAIVEQMRKDEIEHGEEARALGAAEMPGPIRGVMKMMSRVMTTVAYRL"
     gene            complement(8488..>8820)
                     /locus_tag="VFA14_05255"
     CDS             complement(8488..>8820)
                     /locus_tag="VFA14_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007877935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HZS80816.1"
                     /translation="EVVLLGTGGCTAYDVVVILRKSGQDVRGCEVTLKSERAQRDPKV
                     FTRIHFHFTVRGRNLKQNMVERAIKLSHDKYCSASIMLEKTAEMTHSFEIIEDLSDEV
                     LVQTQQQQ"
BASE COUNT         1752 a         2465 c         2529 g         2074 t
ORIGIN      
        1 ggcgcgtttg ccgctgggcg acgtgcgcca gcattcgccc gccgtcatgc tcaatatcct
       61 cggcgatgtc tggtacgagg gcgatagcgg cgcgatgcgc gaaccggcat gggacaagat
      121 tctggcattg ccgggcgcgc atttgcatct gtatggcaaa gcgcaggcgc gccgtgcgcg
      181 caagatgggt cacatcactt ttgtcgcgcc gaccatggcg caggtgcagg tgcaactggc
      241 ggcggcatgc gccattctcg gtatcacgct gtagctgagc agccatgatt gcggttgata
      301 tggatgcagt acggctggcg gcgcgcaaac tggaggccgg ccggctggtg gcatttccga
      361 ccgagacggt ctatggcctc ggcggtgacg ccgggaacgc cgttgccatc aacgccattt
      421 atgcggccaa aggccggccg gctaaccatc ctgtgattgt ccatatcgcg cctgaggccg
      481 atatttctga ttgggcgtca gtggtgccgg cgcaggcaca tgcactgacc gcggcgtttt
      541 ggccgggtcc gctgacgctg gttctcaaac gtgcagcgca catttcggca gccgtttcag
      601 gcggtcagga ttcgatcggc ctgcgttgcc cgtcacatcc ggtggcgcaa gccctgttgc
      661 gcgaattcca gggaggcagg ggcggcctcg ctgcaccgtc ggccaacaaa ttcgggcatg
      721 tcagcccaac tacggcggca catgtgcgtg aggaattcgg caatgatgcc gggggtcttg
      781 tcgattatgt gctggatggc ggtcagagcg aggtcggtat cgaatcgacc attctcgatc
      841 tgtcgcgcgt gacaacacat ggtgctgtgc tgctgcgtcc cggacaaatc agcatcgatc
      901 agattgccgc cgtgctgggc gtgacgccca tggcaccgga tgccgctgcg ccgcgcgcat
      961 cgggaacgct ggatggacac tatgcgccgc atacgccggt agcgcaagtg ccggcgcagg
     1021 aattgccgca gttgatgcag gcgcttgctg atcgtcattg caggttcgcc gtgattcatc
     1081 gcacggtggc agtgccatcc ctgtcggcat cgtttgccat gtcgcacgat gctggctatt
     1141 atgcgcatga tttatatgcg gcattgcggg agatggatca gtgtggtgct gatatcattc
     1201 tggtcgaggc gcttccccat atgtcggagt ggcaaggcat caacgaccgc ctgcgtcggg
     1261 ctgccttcga ctcgaccgga attcttgccc gcctgctggc ggcttgacct gtttttttcg
     1321 gactggaata atccgtccgt ctgtatccct acctcaaact catcatgcgc cgctcacttg
     1381 cattgctcac ggtactcgcc gctttttcgg ctgctcttcc tgctgatgcg cagcaactcc
     1441 agaaacttcc gaccatcacc ctgaacgttg gcctgtatct gatcgaggcc gaagtcgcct
     1501 cgaccgaggc gcagcgcaac cgaggtttga tgttccgtaa cagtatgggc gccaatgaag
     1561 gcatggtgtt tgtgtacggc gctccggcag aggtctgcat gtggatgaaa aacacagcga
     1621 ttccactttc cgtcgccttc atggatgccg ccggtcacat catcaatatc gaggaaatga
     1681 aggaacagac cctggattcg cattgcgcga ccagagctgc cagttatgcc ctggagatga
     1741 acaaaggctg gttcaggcag aaaaatatca agccgggcat gatgatacag ggtttgccgc
     1801 gctgatcagc gtgactatgc aatgccgaga atcacaggca gaggcagtga ctgaggtcat
     1861 tgtctgcctt ccacatcgcc agacgtgggc caatagtggg aatccggtgt ggcgcgcgca
     1921 ccaaaaattg cctgaccaac acgcacgacc gtcgcaccct cctcgatggc gatctcgtag
     1981 tctcccgaca tgcccatcga cagttcgttg agcgcaatgc cttccggagc ctcctgacgc
     2041 agccggtcac gcaggctgcg tagcagaacg aagcattgcc gcaccctgac gggatcggta
     2101 gacagcagcg ccaaagtcat gaggccgcgc acccgcagcg ccggaaaaga cggcaatgcg
     2161 cgcaggaaaa cggcaacctc atctgggtgg agtccgtact tgctggcttc tcctgaagtg
     2221 ttgacctgca cgaatacatc cagtgagcgt ccttcaagct gcaagcgccg ctccaacgct
     2281 tcagctaccc gcaggctgtc caatgcttga aactcggaag cgcagcgcgc caccagtctg
     2341 gctttgttgg tttgtagatg accgatgaca gaccagtgaa ggtcgttcag atccgtcgtg
     2401 gcctcccact tgcggtatgc ttcctgcggc ttgttttcac caagtagccg acagcctgcg
     2461 gcgtaagaca ggcgaatact ggcctcaggc ctgctcttac tcaccggcag cagccgtaca
     2521 ctctctgggt cgcgtcccac acgccggcat gcggcggcga tccgcccatg cacatctgcc
     2581 agattacgac ggaaatcttc ggccgacccg gcctgcggcc agcgcccgtg catctcatgt
     2641 cgggtttggg tgtccattgg catgggggtg tcatgcactg cttgacacat gctgtccgaa
     2701 aatacaagaa cccgccggac caactgggcg ggtcaaggtt gctggactga cagcttgtct
     2761 gatcaggctg ccagtgcctt gatggcagca gccagacggc tcttgtggcg agccgccttg
     2821 tttttgtgaa tgattttttt gtcagcgatg ctgtcgatgg tcgacacgga agtccggaac
     2881 acggaagtcg cggcaatctt gtcgcctgtt tcgattgctt tgcgtacagc cttgattgct
     2941 gtgcgcagca tcgatcgctg gctggagtta tgcgcgtttt gtttgacagc ttgacggaca
     3001 cgcttgcgtg cctgtgcggt atttgccatg aatgatcgtc ctaaatcgag agcatcgcag
     3061 ccctgatgac ttgcgaagcc aaactctgta aagtttcaga ttgtagccat ttttcccgca
     3121 actggcaaat accgtgctat tttttacttc cggcatgttt tccccgatga catgaaagag
     3181 cgcctgcagg accgaaaacg aaagctataa tgccgtccca tgaatttgca taaaacgctt
     3241 gtcgccatct ccggcatgac gatgctctcc cgcattaccg gtctcgcgcg cgagattttg
     3301 tttgcacgtg cctttggcgc ttcctcctat accgatgcgt tcaacgtcgc tttccgtatc
     3361 cccaacctgt tgcgccggct gtttgctgaa ggcgcttttt cgcaggcttt cgttcccatt
     3421 ctggctgaat ataaaaacca gcgaggcaat gccgctacca ggcatctggt cgaccatgtg
     3481 gcaacggtgc tgatctggac gatgctggcg accagcatca tcggcatctt gctcgcgccg
     3541 gtgatggtgt atttcatcgc gaccggcctg aaatacgatc agggtgcctt tgatgcttcg
     3601 gtcgtcatga cccgtatcat gtttccgtat atcggcttca tggcctttgt ggcgttggcc
     3661 ggcggcattc tcaatagctg gcgcgaattc aggatcccgg ctttcacccc ggtgttattg
     3721 aatctgtctt tcatcgtcgc tgcgctgtgt gtcgcgcctt acctggatca gcccatctat
     3781 gccatggcgt ttgccgtgtt cgtcggcggc atgttgcaag tgctgctaca gattcccgcc
     3841 ttgatcaagg tcggcatgct gccgcgactg tatgtcaatc cggcgatcag ccttgctgac
     3901 gaaggcgtca aacgcgtgct caaaaaaatg gggccggctg tgttcgccgt gtcggccgcg
     3961 cagatcagcc tgatgatcaa caccaacatc gcttcgcggc tgacgcatgg cagtgtgtcg
     4021 tggctgtcct acgccgaccg tctgatggag tttccaactg cgctgctcgg cgtggcgctg
     4081 ggtaccattc tgttgcccag cctgtcaaac gcccatgctg aaaacgacat ggccgaatac
     4141 tccgcgctgc tcgattgggg cctgcgtctg agctttctgc tggccatgcc ctgtgcggtt
     4201 ggaatggcga cgctggcgga tcccttgacc gcgacgctgt tccattatgg tcaattcgat
     4261 acgctgtcgg taaagatgac tggccatgcg ctgatcgcct acggcgtcgg tctgaccggt
     4321 ttgatccttg tcaagattct cgctcccggc ttctacgcca ggcagtatat atggacaccg
     4381 gtcaagatcg ccatcggcgt attgatcgcg acgcagttga tgaactgctt tttcgttcct
     4441 tggatggcgc acgctggcct ggcgctgtcg atcggtctcg gcgcttgtct gaatgccgca
     4501 ttgctgttcc ggggattgcg ccatcgccat ctctatacgc cgcagcccgg ctggcgcctg
     4561 tttttgatca agctgaccgg cgccttgttc ctgctgggcg gcgtagcgct gacagtggcg
     4621 cggcatttcg actggatcgc catgcgcgcc catccggccg agcgcatcgg cgcattgttg
     4681 ctggttcttg tagcatgcgg cgtgacctat ttcggtgccc tgatggcgat gggtttccgc
     4741 tacgacgatt tcaagcgcat ccgtctctag atgtttagtc ccggcacttg acgaagggat
     4801 caatcctgaa gcttgatccc ttcgtcaagt gccgcctgcg caggctcctc gccggtatat
     4861 ccctgatcgg tcacgctcta gaaataagac ggccccacaa aagcggggcc aaaacgaacc
     4921 tagtgtcatc accggtatgg tgaccgggag agggcttagc ctagggctgc atgtgtagta
     4981 taccatctca atgaacaatg atcaaaatcg gggtgcgatg aactggacag accttgaaaa
     5041 acacttgaat cgcgtattcc atcatgaacc gctgctgtgg ctgacgccgg cgttaatcga
     5101 ccaacataca ctcggcttgc aggccatcgg ctggcttgat cctcagctca tagactggtt
     5161 aaaccatcat gaccgcctgc cggtagcgtg gcagtctgga agaacattta ggccatatag
     5221 cagcgatcaa gctgcgccag ctatcggcgt aaagccttag caatttcttc aaatgcaaga
     5281 acagggattt tatttgtgac aatgcagccg ctttttcccg caggcttagc agcgattacg
     5341 accgaattct tctgcgactc ttcaagtgac cgacctttgg ttgggtttcc ttctttaaca
     5401 tgctgtaacg ctttctgcgt ggccgcctct ttagtgctag cagacacaat catttccagc
     5461 acgtgcgggt ggtccaatgt ctgtacgtac actttataaa gtcccgcatc gccgtacacc
     5521 ttttctacta tcgctgttcc tgttggatat tcatttgctg cttctttgat ttcattatcg
     5581 tacatccagc gataaagcca aatgtacgtt tctactctga taaggcgtgg attatgtttg
     5641 gccggacaat gttgcattgc cgcgtcgttg ccaggagaat atttcacaag cgggcatatt
     5701 ttgatagcat caccaacata tggcgctcac acatctcgag tatttttcat gtctggttcg
     5761 tcaggatagc tcgattccct tgtttgaatc ggccctggcg ttgggacagg atgcgtatcc
     5821 cgatctggat ttttctgctt gccagatgga gatggataac ttggccttga agttgaggca
     5881 acgtttattg tcggatgcaa cgcacattca gaaattgcgt gtactcaacc gcttcttttt
     5941 tcaggaactg gctttcgccg gcaacgccaa caatttttat gatcccgaca acagttacat
     6001 ccaccgcgtg atgatgtcgc ggcgcggcat cccgatctcg ttggcggtgg tgtacatgga
     6061 actggggcaa cagatcggtt tggatataaa aggcattccc tttcccggtc attttttgat
     6121 gaagctctcc gtgcaatccg gcgagatcgt actcgatccg atgaacggcg ccagcctgtc
     6181 gcgcgaagag ctagaggtgc gtctgggact ctatttggaa cggcagggaa aaagtggtga
     6241 actgccgttg gcagtctgcc tgcgtgccgc tcacccgcgt gaaatcctca cgcgcatgct
     6301 gcgcaacttg aaagtgattt ttgccgaagc acaacgctgg gagcatgtac tcgcggtgca
     6361 gaagcgtctg gtgattttat tgccgaatga aattaccgag cgccgcgacc gcggcctggc
     6421 ctatgccaac ctggcagagc cgcaggcggc gttgaaagac ctggaagcct atctggcctt
     6481 gcgacccgat gccgacgacg ccgacatcat gcgccagcgc ttgccggagc tgcgtcatta
     6541 cagccggcgt cccggctaag aaccgtacat gtaagtttct cccccgattg atctcattac
     6601 tccctctgaa gttctctcat cagggaggat tacccttgcg gtaaaatggt agggtttcat
     6661 gtctgcggca caggcggtca ttgaggagag aagagaaagc gatgcgcgat gtaataaata
     6721 tgacccgccc ggatgactgg catttgcatg tgcgtgacgg cgcgacgctg gcgagtgtgc
     6781 tgccgcatac cgctcatcaa ttcgcgcgcg cgatcatcat gccgaacctg aagccgccgg
     6841 tcacgaccgt ggcgttggca agcgcctatc gcgatcgcat tctggcagcg ctaccggctg
     6901 gttcttcgtt cgagccgctg atggcgctgt atctgaccga tgacacgccg ccggaagaaa
     6961 ttcgcaaggc cagggacagc ggtttcgtgc atgcggtcaa aatgtatccg tccggcgcca
     7021 ccaccaattc cgatgccggc gtgaccgatc tgcgcaagtg cggcaagacg ctggaagcgt
     7081 tgcaggaagt cggcatgcct ttgctgatac atggtgaagt caccgatccc gccatcgata
     7141 tttttgaccg ggaagccgtg tttatcgaac gtgtcatgat gccgctgcgc aaggatatgc
     7201 ctgaactgaa aatcgtcttc gaacacatca ccaccaggga tgccgccgac tatgtgatgg
     7261 cggcggaagg gccgacggcg gcgacgatca cagcccatca tttactgtac aaccgtaatg
     7321 aaatctttaa gggcggcatc cgtccgcatt attactgcct gccgatactc aagcgcgaga
     7381 tgcatcggca ggcgctgatc gcggcggcgg tttccggcag tagccggttt tttctcggta
     7441 cggattcggc gccgcatgcc aggggtttga aagagcatgc ctgcggctgt gccggctgct
     7501 ataccgcatt gcatgcgctg gaattgtatg cgcaagcctt cgatcaggcg ggcgcgctgg
     7561 acagactcga agccttcgcc agtttcaacg gtccggcttt ctacaatcta ccgtgcaatc
     7621 aaggcagtgt gaccttgcgt cgcgagcgct ggaccatccc gaccgagctg ccgatgggtg
     7681 agtcgatgat ggtgccattg aatggcggcg aaacgatgca ttggaaaatg atgtgatata
     7741 tcgcacaggc gaagataatg tatttttaac gggaatccat gctcatgcaa ttcattgatc
     7801 aattgattac cgatttggac aaagccctgc gctcagtcag cggtgtgacg gtttcatcgc
     7861 gttcgaatcc ggcgatgcac attaccgatg ccgcaatgag cgatgaggaa aaacgtcata
     7921 gcgcgggatt gatgcgcgtc aatcatgtcg gtgaggtctg cgcgcaggct ctgtacgatg
     7981 cgcaaggccg ttttgcgcag acgcaagcct tgaagcagct attcgctcat gccggcctcg
     8041 aagaggaaga tcatctggcc tggactgccg aacgcttgcg tgaactcggt tcgcacacca
     8101 gcctgctcaa ccccttgtgg tacgcgggtt cttatgtgct tggcgcgatc gccgcgcata
     8161 tgggcgatgc gcgcagtctc ggtttcgttg tcgagaccga gcgtcaggtc gaacagcatt
     8221 tgaccagtca tctgggaaaa ttgccgctgc aggacgccag atcgcgcgct atcgtcgaac
     8281 agatgcgcaa ggatgagata gaacacggcg aggaggccag ggcgctaggc gctgccgaga
     8341 tgccaggacc gattcgcggc gtcatgaaaa tgatgtccag ggtcatgaca acggtggctt
     8401 accgtttata gacgcgaacg acgccaatgt cgttcgatct ggccaccatc cgtcccagtg
     8461 tcgtaaaaca atgggtgcgc cggttgttta ctgctgttgc tgcgtttgta ccaatacttc
     8521 gtcactgaga tcttcgatga tctcgaacga atgcgtcatt tccgccgttt tttccagcat
     8581 gatggatgcc gagcagtatt tgtcatgtga caacttgata gcgcgttcca ccatattctg
     8641 cttcaggttg cggccgcgca cggtgaagtg aaaatggatc ctggtgaaaa ctttcggatc
     8701 acgttgcgcg cgttccgact tgagcgtgac ttcacagccg cgaacatcct ggccgctttt
     8761 gcgcaaaatc acgacgacat cgtaagccgt gcaaccgccg gtgcccagca ggacgacttc
//
LOCUS       DASRDH010000227         2022 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1300147,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000227 DASRDH010000000
VERSION     DASRDH010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1300147"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..589)
                     /locus_tag="VFA14_05260"
     CDS             complement(<1..589)
                     /locus_tag="VFA14_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZS80817.1"
                     /translation="MMLDFLGAIGRTLREFKFIQGVGFAARLFWMVMRSSGGLWRRPR
                     LMTDQIHFIGNYSLVIIAVSGLFVGFVLGLQGYYTLNKYGSEQALGLLVALSLTRELG
                     PVVTALLFAGRAGTSLTAEIGLMKAGEQLSAMEMMAVDPLQRVIAPRFWAGVLAMPVL
                     AAIFSAVGVIGGYIVGVQLIGVDEGAFWSQMQGGVD"
     gene            complement(593..1390)
                     /locus_tag="VFA14_05265"
     CDS             complement(593..1390)
                     /locus_tag="VFA14_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006461560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZS80818.1"
                     /translation="MPNIVEISDLHFAYGDRPILSGLCMDFPRGQVIAVMGGSGSGKT
                     TILRLIGAQLRPQSGSLTVDGENVPTLDTAALYLLRRKMGMLFQNGALFTDMTVFDNV
                     AFPLREHTALPEELIRDLVLLKLNAVGLRNAAGLKPAEISGGMARRVALARAIALDPA
                     LIMYDEPFAGLDPISMGITANLIRRLNDALGSTSILVSHDVNESFAIADYVYFLSQGR
                     IVARGTPDEMRVSQDPYVRQFVHAETDGPVPFHYPGRSLAEDVGLGG"
     gene            1507..>2022
                     /locus_tag="VFA14_05270"
     CDS             1507..>2022
                     /locus_tag="VFA14_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HZS80819.1"
                     /translation="MSLKCGIVGLPNVGKSTLFNALTKAGIAAENYPFCTIEPNIGIV
                     EVPDPRLHALAEIVKPERILPATVEFVDIAGLVAGASKGEGLGNQFLSHIRETDAIIN
                     VVRCFEDPNVIHVAGKVSPLDDIAVIQTELALADMGTIDKVIHREQKKARAGDHDAAR
                     LVALLERILPAL"
BASE COUNT          489 a          646 c          556 g          331 t
ORIGIN      
        1 catccacgcc accctgcatc tgtgaccaga acgcgccttc atccacaccg atcaactgca
       61 caccgacaat atagccgccg atgacaccga cagcactaaa aatcgcagcc agcacgggca
      121 tggcaagaac accagcccaa aagcgcggcg caatgacgcg ctgcaaggga tcgaccgcca
      181 tcatctccat cgccgacaat tgctcaccgg ctttcatcag gccgatttcc gcggtcaacg
      241 aggtgccggc gcgaccagcg aacagcagcg ccgtcacgac cggacccagt tcacgcgtca
      301 acgacaacgc caccagcaaa cccaatgcct gttccgaacc gtatttgttg agcgtgtaat
      361 agccctgcaa acccagcacg aaaccgacaa acaggccgga cacggcaatg atcaccagcg
      421 aatagttgcc aatgaagtgg atctggtcag tcatcaggcg cggacgacgc cacaggccgc
      481 cggacgaccg catcaccatc cagaacaggc gcgcggcaaa accgacgccc tgaatgaatt
      541 tgaattcgcg cagcgtgcgc ccgatcgcgc caaggaaatc aagcatgatg acttaccccc
      601 ccaggccgac atcctcggcc agcgaacggc ccggataatg gaaaggcacc ggaccatcgg
      661 tctcggcatg cacgaattgc ctgacgtaag gatcctgcga cacgcgcatt tcgtcgggcg
      721 tgcctcgcgc gacgatccgg ccctgtgaca ggaaatagac atagtcagca atcgcgaacg
      781 actcgttgac gtcatgcgac accaggatcg aggtcgaacc caatgcgtcg ttgagcctgc
      841 ggatcagatt ggcggttatt cccatggaaa tcggatcgag gccggcaaac ggttcatcgt
      901 acatgatcag cgccggatcc agcgcaatgg cccgcgccag cgccacgcgg cgcgccatgc
      961 cgccggaaat ctcggccggc ttgaggcccg cggcattgcg caggcccacg gcattgagct
     1021 tgagcagcac cagatcacgg atcaattctt ccggcagagc ggtatgctcg cgcagcggaa
     1081 acgcgacgtt gtcgaacacc gtcatgtcag taaacaaagc gccgttctgg aacagcatgc
     1141 ccatcttgcg gcgcagcagg tacagcgcgg cagtatccag tgtcggcacg ttctccccat
     1201 ccacggtcag gctgccggat tgcggacgca gctgagcgcc gatcagacgc aggatcgtgg
     1261 ttttgccgga gccggaaccg cccatgacgg cgatcacttg tccacgcggg aaatccatgc
     1321 aaaggcccga caggatcgga cgatccccat aagcaaagtg caggtcgctg atttctacga
     1381 tattaggcaa gagggctacc aggaatttcc aaagcttgac attgtagagc aaaatgaacg
     1441 gtcgcttatc atcggcctgg acagcccttg ctacaatacc gcttttgatc aagaaagaat
     1501 gttgtcatga gcctcaaatg cggcatcgtg ggcttgccca acgtcggtaa atcgactctt
     1561 ttcaatgccc tgaccaaggc cggcatcgct gctgaaaact atccgttctg cacgattgag
     1621 ccaaacatcg gcatcgtgga agtacccgat ccgcgtctgc atgcgctggc agaaatcgtc
     1681 aaaccggaac gcatcctgcc ggcaacggtc gagttcgtcg atatcgccgg cctggtggcc
     1741 ggcgcctcca aaggcgaagg cctgggcaac cagttcctgt cgcacatccg cgaaaccgac
     1801 gccatcatca acgtcgtgcg ctgctttgaa gacccgaacg tgatccatgt ggctggcaag
     1861 gtcagtccgc tcgacgacat cgccgtgatc cagaccgaac tggcgctggc tgacatgggc
     1921 accatcgaca aagtaatcca ccgcgaacag aaaaaagcgc gcgcgggcga ccatgacgcc
     1981 gccagactgg tcgcgctgct ggaacgcatc ctgccggcgc tc
//
LOCUS       DASRDH010000228         3392 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1220620,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000228 DASRDH010000000
VERSION     DASRDH010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 3392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3392
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1220620"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..126
                     /locus_tag="VFA14_05275"
     CDS             <1..126
                     /locus_tag="VFA14_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007880366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HZS80820.1"
                     /translation="AVYIVQAIKRVQGMRLIGPAWRTRKMKVLKGVPATNRIHKE"
     gene            192..626
                     /gene="rnhA"
                     /locus_tag="VFA14_05280"
     CDS             192..626
                     /gene="rnhA"
                     /locus_tag="VFA14_05280"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HZS80821.1"
                     /translation="MGKIVIYTDGACKGNPGKGGWGALLIAGDHEKELCGGENGTTNN
                     RMELKAVIEALGILDRPCDVTVYTDSQYVQKGISEWIHGWKARGWKTAAREPVKNVDL
                     WQALDAAQARHKIEWRWVRGHAGHPGNERADRLANRGVDSVA"
     gene            646..1380
                     /gene="dnaQ"
                     /locus_tag="VFA14_05285"
     CDS             646..1380
                     /gene="dnaQ"
                     /locus_tag="VFA14_05285"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HZS80822.1"
                     /translation="MRQIVLDTETTGLNPRTGNRVIEIGCVELLNRRLTGNDLHFYIN
                     PERDSEEGALAVHGLSTEFLSDKPRFAEIADAFRDYVRGAEIIIHNAPFDVSFLDAEF
                     KLLGLGPFQEHVGNIIDSLKMAMEQHPGRRNSLDALCDRYGISNAHRTLHGGLLDATL
                     LAEVYLAMTRGQNSLAMDSADDSKDTAATVELAPLADVIVVAASADELALHEAMLDTL
                     DKEIKGLCVWHAAPMEETPPSTVRKS"
     gene            complement(1503..1625)
                     /locus_tag="VFA14_05290"
     CDS             complement(1503..1625)
                     /locus_tag="VFA14_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS80823.1"
                     /translation="MSPLKTKTKTKTKTKTKTKTKTKTKTKTKTKTEQEMILKI"
     gene            1628..2293
                     /locus_tag="VFA14_05295"
     CDS             1628..2293
                     /locus_tag="VFA14_05295"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HZS80824.1"
                     /translation="MKALQQFIHFAEKFPGGIWGAKDTNSHYLYMGSAYCDLLNLPMT
                     LKIEGLLDSELPHPCSEFEKEFQAHDRLVEVTQEMKRSLEIHPYGKDQVLSAYCFEKH
                     PLFNENKEVIGTIIHGYPALNLRFDRGKMNMGSFIFGVPDDTLSKKEWDFVYFVMRGY
                     STKEMARLSEVSPGTITSRQNSVFNKLGIDTLAQLKQMAMAKGWDNYIPTDLLHKRHV
                     GLW"
     gene            complement(2356..>3392)
                     /locus_tag="VFA14_05300"
     CDS             complement(2356..>3392)
                     /locus_tag="VFA14_05300"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZS80825.1"
                     /translation="DMAPQTAMLRGGVEIVIATPGRLLDHVQQKTVNLSQTGILVMDE
                     ADRMLDMGFLPDLQRIIDLLPRQRQNLMFSATFSPEIKKLAASFLNNPVTIEVARSNA
                     TAERVTQTMYKVSEDAKSDAVAYIIRERCLKQVIVFSNTKIGASRLVHHLENGGIKAS
                     AIHGDKSQSERMAALEAFKQGQVEVLVATDVAARGLDISDLPCVINVDLPYNAEDYVH
                     RIGRTGRAGASGDAISLFSDKDERLLVDIEKLIRIKFDRSELSGFVPSQRGGRGNYNE
                     RHGNGSVRMPRDTQSWGRNNGAPRKEKVDPWFFMPYEPTAASVPVEKPAADKQPKHRV
                     AALLGGMSKR"
BASE COUNT          795 a          935 c          934 g          728 t
ORIGIN      
        1 gcggtctata tcgtgcaggc gatcaagcgc gtccagggca tgcgcctgat cggcccggcc
       61 tggcgcacac gaaaaatgaa agtgctgaaa ggcgtcccgg ctactaaccg catccataaa
      121 gaataaaatg ctgccctcga cgcatcgagc cgcaacgacc agacaacgaa acagatatga
      181 ataaagaaaa aatgggcaaa atcgtcattt acaccgatgg cgcctgcaag ggaaatccgg
      241 gcaaaggtgg ctggggcgcg ctgttgatcg ccggcgacca tgagaaagaa ctctgcggcg
      301 gtgagaacgg caccaccaac aatcgcatgg aactcaaggc cgtcatcgag gcgctgggca
      361 tactcgaccg tccctgcgac gtgaccgttt ataccgacag ccagtatgta caaaaaggca
      421 tcagcgaatg gattcacggc tggaaggcgc gcggctggaa aactgctgcg cgcgaaccgg
      481 tcaagaatgt cgacctgtgg caggcactcg acgccgcaca ggcgcggcac aagatcgaat
      541 ggcgctgggt cagaggccat gccggccacc ccggcaacga acgcgccgac cgactggcca
      601 atcgcggcgt cgatagcgtc gcttgaagag catcaggaat cccccatgcg acaaatcgtt
      661 ctcgatacag aaaccactgg cctgaacccc cgtaccggca accgcgtgat tgaaatcggt
      721 tgcgtcgaat tgctgaaccg ccggcttacc ggcaacgatc tgcacttcta catcaacccc
      781 gagcgcgatt ctgaagaagg cgcgctggcg gtgcatggcc tgtccactga atttctcagc
      841 gacaagccca ggtttgcgga gattgccgac gcattccgcg attacgtgcg cggtgctgag
      901 atcattattc acaatgctcc cttcgacgtc agtttcctcg atgctgaatt caaactgctc
      961 ggtcttggcc ctttccagga acatgtcggc aacatcatcg actcgctgaa gatggccatg
     1021 gagcagcatc ctggccgccg taattcgctc gatgcgctat gcgaccgtta cggcatttcc
     1081 aacgcgcacc gcacgctgca cggcggcctg ctcgatgcga cgctgctggc ggaagtctat
     1141 ctggccatga cacgcggcca gaacagcctg gccatggatt ctgctgacga cagcaaagac
     1201 acagccgcca cagtcgaact ggcgccgctg gctgacgtca tcgtggtggc cgccagcgcg
     1261 gatgaactgg cgctgcacga agccatgctc gataccctcg acaaagaaat caaaggccta
     1321 tgcgtatggc atgcagcgcc gatggaagaa actccgccgt caactgtgcg aaaatcatga
     1381 cacgcgcttg gaatgggttc caagagtcta ttcacaatct ttttcctgcc ggcgcaggag
     1441 tttttctact gaagggcagg cgggcttttc agacttttct catagacaaa taaaaacgga
     1501 tctcatattt tcagaatcat ctcttgctcg gtcttggtct tggtcttggt cttggtcttg
     1561 gtcttggtct tggtcttggt cttggtcttg gtcttggtct tggtcttggt cttcaaagga
     1621 gacaatcatg aaagcattgc agcaatttat ccattttgcg gagaagttcc caggaggaat
     1681 atggggggcc aaagatacga attcccatta tttgtatatg ggttccgctt attgtgatct
     1741 gttgaacttg cccatgacct tgaaaattga aggcttactg gatagcgaac tgccccatcc
     1801 gtgttccgaa ttcgagaaag agtttcaggc acatgatcgc ctggttgaag tcactcaaga
     1861 aatgaagcgg tctctggaaa ttcatccata cgggaaagac caggtgttat cagcttattg
     1921 cttcgaaaaa cacccattgt tcaacgagaa taaagaagtg atcggcacga ttatccacgg
     1981 ttacccggcg ctgaatctac gattcgatag gggtaagatg aatatggggt ctttcatttt
     2041 cggtgtaccg gacgataccc tgtcaaaaaa agagtgggat ttcgtctatt tcgtgatgcg
     2101 cggttattcg accaaagaaa tggcgcgctt gtcagaagtc tcaccgggta cgattaccag
     2161 caggcagaac tccgtcttca acaaattagg catcgacacg cttgcccagc tcaaacagat
     2221 ggcgatggcg aagggctggg acaactatat cccgaccgat ctgctgcaca agaggcatgt
     2281 cgggctgtgg tgacaggatc tttgcgccgc ctgaaagagc gaccagacga ccccgcagga
     2341 gatcgtcaac aggttctaac gcttggacat accacccaac agcgccgcca ccctgtgctt
     2401 gggctgcttg tcggcagccg gtttttccac cggaacggaa gcggcagtcg gctcgtaagg
     2461 catgaagaac catgggtcga ccttttcctt gcgcggcgcg ccattgttgc ggccccagga
     2521 ttgggtatca cgaggcatgc gaacgctgcc attaccatga cgttcattgt agttgccgcg
     2581 gccaccgcgc tgagatggca cgaaaccgct cagttccgaa cggtcaaact tgatcctgat
     2641 cagtttttcg atgtcgacca gcagacgctc atccttgtca gaaaacagcg aaatcgcatc
     2701 gcctgaagcg ccggcgcgac cggtacgacc aatgcggtga acataatctt cagcgttgta
     2761 cggcaaatcg acgttgatga cgcaaggcag atcgctgata tccagaccgc gcgcggcgac
     2821 gtcggtcgcc accagcacct cgacctggcc ttgtttgaag gcttccagcg cggccatgcg
     2881 ctcactctgg gatttgtcgc catgaatcgc agaagccttg atgccgccgt tttcgagatg
     2941 atgcaccagc ctggaagcgc cgatcttggt gttggaaaac acgatcacct gtttcaagca
     3001 gcgctcacga atgatgtacg cgacggcatc gctcttggcg tcttcgctga ctttgtacat
     3061 ggtctgcgtc acgcgctcgg cggtggcatt gctgcgcgcc acttcgatgg tgaccggatt
     3121 gttcagaaag ctggcggcca gcttcttaat ttccggcgaa aatgtcgccg agaacattag
     3181 attctggcgc tgcctgggca gcaaatcgat gatgcgttgc aagtccggta aaaagcccat
     3241 atcgagcatg cggtcggctt catccatcac cagaattccg gtttgcgaca gattcacggt
     3301 cttttgctgc acatgatcga gcagacggcc cggcgtggca atcacgattt caacgccgcc
     3361 acgcagcatc gcggtttgcg gcgccatatc ga
//
LOCUS       DASRDH010000229         3970 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1073470,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000229 DASRDH010000000
VERSION     DASRDH010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 3970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3970
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1073470"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /gene="minC"
                     /locus_tag="VFA14_05305"
     CDS             <1..418
                     /gene="minC"
                     /locus_tag="VFA14_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018058221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HZS80826.1"
                     /translation="APAGAAEETPKTTPAPTPAPAAASVVTTMVIDTPVRAGQRIYAR
                     NADLVIMAAVNNGAEIIADGSIHVYAPLRGRALAGASGNTSARIFAVNLEAELVSIAG
                     VYRTFENGHASALARQPVQVCLNGDRIDVLPIHTGK"
     gene            462..1277
                     /gene="minD"
                     /locus_tag="VFA14_05310"
     CDS             462..1277
                     /gene="minD"
                     /locus_tag="VFA14_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013236197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HZS80827.1"
                     /translation="MAKIVVVTSGKGGVGKTTSSASFASGLALRGHKTAVIDFDVGLR
                     NLDLIMGCERRVVYDLINVVNQEATLNQALIKDKHCDNLFILPASQTRDKDALSEEGV
                     ERVLGDLDKMDFEYIICDSPAGIEHGAVMALTFADEAIIVTNPEVSSVRDSDRILGII
                     QAKSRRALNGSEPVKEHLLITRYAPKRVEAGEMLSYADVQEILRIPLISVIPESESVL
                     HASNQGKPAIHLQESDVAEAYHDLVGRFLGEDLPLRFISNEKPSLLQRIFGGK"
     gene            1279..1551
                     /gene="minE"
                     /locus_tag="VFA14_05315"
     CDS             1279..1551
                     /gene="minE"
                     /locus_tag="VFA14_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006465131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HZS80828.1"
                     /translation="MALLSFLFPPKAKSAVAAKERLQIIIARERSGREGQGHDFLPAL
                     HKELIEVISKYTKVNANDIKISLDRQGNLEVLDVNVVLPDSDTETS"
     gene            1779..2207
                     /gene="rplM"
                     /locus_tag="VFA14_05320"
     CDS             1779..2207
                     /gene="rplM"
                     /locus_tag="VFA14_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008919631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HZS80829.1"
                     /translation="MKTFSAKAHEVRREWFVIDATDKVLGRVASEVALRLRGKHKPEF
                     TPHVDTGDFIVVINAGKLRVTGAKATEKIYYRHSGYPGGIYATSFLKMQQRFPGRALE
                     KAVKGMLPKGPLGYAMIKKLKVYADDSHPHAAQQPKALEL"
     gene            2217..2609
                     /gene="rpsI"
                     /locus_tag="VFA14_05325"
     CDS             2217..2609
                     /gene="rpsI"
                     /locus_tag="VFA14_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HZS80830.1"
                     /translation="MIGNYNYGTGRRKSAVARVFIKSGSGSIIVNGRPANEYFSRETG
                     LMVIRQPLELTNNVERFDILVNVHGGGESGQAGAVRHGITRALIDYDATLKPELSKAG
                     FVTRDAREVERKKVGLRKARRAKQFSKR"
     gene            2743..3702
                     /gene="gshB"
                     /locus_tag="VFA14_05330"
     CDS             2743..3702
                     /gene="gshB"
                     /locus_tag="VFA14_05330"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005667797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HZS80831.1"
                     /translation="MKVAFLTDPLATFKTYKDSTFAMMREAARRGHVIYAFGQADIVL
                     ESGLVTANAARITLTGDADDWYRANTPTAAFLSGFDAVVIRKDPPFDMEYIYTTYLLE
                     LAEKQGARVFNRPAAIRSHNEKLAIAQFPQYTAPTLVTRDVRRLRAFHAEHKDMILKP
                     LDGMGGTGIFRIGRDGMNLGAAIEMLTDNGNRTIMAQSFIPEIASGDKRILLIGGKVV
                     PHMLARIPQGGEVRGNLAAGGLGVTQPIAPHELEIAEALAPVLAQRGLLLVGLDVIGS
                     YLTEINVTSPTCFQEITQQSGFDVAAMFVDALEAAVAEPGLAT"
     gene            3717..>3970
                     /locus_tag="VFA14_05335"
     CDS             3717..>3970
                     /locus_tag="VFA14_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018057997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HZS80832.1"
                     /translation="MVGILLLTHAPLGQAFITAVTHVFCDVPERMEAIDVIADQSPSE
                     VQKLAADAVCRLDDGSGVLVITDVLGGTPSNCCTSLCAIS"
BASE COUNT          889 a         1151 c         1137 g          793 t
ORIGIN      
        1 cgcgccggcc ggcgcggctg aagaaacacc gaaaaccacc cctgcgccca cacctgctcc
       61 tgccgcagca tcggtagtca ctaccatggt catcgacaca ccggtgcgcg ccggtcaacg
      121 catttacgcg cgcaacgccg atctggtcat catggcagcc gtcaacaacg gcgcggagat
      181 cattgccgac ggcagcatcc atgtctacgc cccgctgcgc ggccgcgcct tggccggcgc
      241 cagcggcaat accagcgcac gcatcttcgc cgtcaacctg gaagcagaac tggtatcgat
      301 cgccggcgtc tatcgtacct ttgagaacgg acacgcgtct gcactggccc ggcagccagt
      361 gcaggtgtgc ctgaacggcg accgtatcga cgtgttgccg atccataccg gcaaatgacc
      421 ggcatttacc tatcaaatct gatttcctaa ggagtgctgc tgtggcaaaa attgttgttg
      481 tgacctccgg taaaggcggt gtcggcaaaa cgacatcgag cgccagtttc gcgtcgggtc
      541 tggctctgcg cggccacaag accgccgtga tcgatttcga cgtcgggctg cgcaacctcg
      601 acctcatcat ggggtgcgaa cgccgcgtag tatacgatct gatcaatgtc gtcaaccaag
      661 aagcaacgct gaatcaggcg ctgatcaagg acaagcactg cgacaatctg ttcatcctgc
      721 cggcatcgca aacccgcgac aaggacgcgc tgtcggaaga aggcgtggag cgtgtgctgg
      781 gtgaccttga caagatggac ttcgaataca tcatctgcga ctcgccggcc ggtatcgagc
      841 acggtgcagt gatggcgctg accttcgccg acgaagcgat catcgtgacc aatccggaag
      901 tatcatcggt gcgcgactcc gatcgcatcc tgggcatcat ccaggccaag tcgcgccgtg
      961 ccctcaatgg cagcgaaccc gtcaaggaac acctgctgat cacccgctat gcgcccaaac
     1021 gcgtcgaagc cggagaaatg ctgtcctacg ccgatgtgca ggaaatcctg cgcatcccgc
     1081 tgatcagtgt catccccgaa tcggaatccg tactgcatgc ctctaaccag ggtaagccgg
     1141 cgattcacct gcaagagagc gacgtggccg aagcctatca cgatctggtc ggtcgcttcc
     1201 tcggcgaaga cctgccgctg cgcttcatca gcaacgaaaa gccaagcctg ctccagcgta
     1261 ttttcggagg taaatgacat ggctttgctt tcctttctgt ttccgcccaa ggcgaaaagc
     1321 gccgtggccg ccaaggaacg cctgcaaatc atcatcgcgc gcgagcgcag cggccgtgag
     1381 gggcagggac atgatttttt gccggccctg cacaaggaac tgatcgaagt catttccaaa
     1441 tacaccaagg tcaatgccaa cgacatcaag atctcgctcg accgccaagg caacctagaa
     1501 gtgctcgacg tcaacgtggt gctgccggac tcggacaccg aaacatcctg accgtgtcag
     1561 acggtcagga cgacgccatg cgggaccggc cagggtctcg ataaaaaatt tgacgctaag
     1621 tcattgaaaa ggttatgatt gcccgcttcc cgtgtgttcc gggaaagatc ataaggaaga
     1681 gtctgcgtcg gcaaggcaag gtcgatcaca ttacgcgcca tggtagccgc agaaccgccg
     1741 gatcgagcga tcaatgttaa tttaactagg aagtcatcat gaaaaccttt tccgctaaag
     1801 cccatgaggt aaggcgcgag tggttcgtga ttgacgcgac ggacaaagtc ctcggacgcg
     1861 ttgccagcga agtggcgctc cgactgcgcg gcaagcacaa accggaattt actccgcacg
     1921 tcgacacggg cgatttcatc gtcgtgatca atgcaggcaa gctgcgcgtc accggcgcca
     1981 aagcaactga aaaaatctac taccgtcaca gcggctatcc gggcggcatc tacgcaacca
     2041 gtttcctgaa aatgcagcag cgttttccag gccgcgcgtt ggagaaagcg gtcaagggca
     2101 tgttgcccaa gggtccgctc ggctacgcga tgatcaagaa gctgaaggtg tatgcggatg
     2161 acagccatcc gcacgccgcc cagcagccca aagcactcga actctaagga atggacatga
     2221 tcggtaacta caattacgga accggccgtc gcaagagtgc ggtggcgcgt gttttcatca
     2281 aatccggttc gggcagcatc attgtcaacg gcaggccggc gaacgagtat ttttcgcgcg
     2341 aaaccggtct gatggtaatt cgtcaaccgc tagagttgac caataatgtc gagcgtttcg
     2401 atatcctggt caacgtccat ggcggcggtg aatccggcca ggctggcgcc gttcgtcacg
     2461 gcatcacccg cgccttaatc gactacgatg caacgctgaa accggaactg tccaaggcag
     2521 gcttcgtgac ccgtgatgcg cgtgaagtcg aacgtaaaaa ggttggtctg cgcaaggcgc
     2581 gccgcgcgaa gcagttctcg aagcgttaat atttaatatc gacgtatcga cgcgtgcttt
     2641 cccggcacaa caaaaaagcc gccggcgaac gcctggcggc tttttgcatt atgcgcaggg
     2701 atctccggcc ttacaccgta atttcttttt gatggctcat gcatgaaagt cgcttttctg
     2761 acggacccgc tcgctacctt caagacctat aaggactcga cttttgcaat gatgcgcgaa
     2821 gcggcccgac gtggtcatgt catctacgct ttcgggcagg ctgacatcgt gctggaaagc
     2881 ggtctggtga cggccaatgc ggcgcggatc acgctcaccg gtgatgccga cgactggtat
     2941 cgtgccaaca ccccgaccgc ggctttcctg tccgggttcg atgccgtcgt gatccgtaaa
     3001 gatccgccgt tcgacatgga gtatatctat accacgtatc tgcttgaact ggcggagaag
     3061 caaggcgcgc gcgtgttcaa caggccagca gcgatccgca gccataatga aaagctggcg
     3121 attgcacagt ttccgcaata taccgcgcca acgctggtca cgcgcgacgt caggcgtctg
     3181 cgtgcttttc atgccgaaca caaagacatg atcctgaagc cgctcgatgg catgggcggc
     3241 acagggattt ttcgcatcgg ccgggatggc atgaatctgg gcgcggcgat cgagatgctg
     3301 accgacaatg gcaaccggac catcatggcg cagagtttca ttccggaaat cgccagtggc
     3361 gacaagcgta tcctgctgat tggcggtaaa gtggtgccgc atatgctggc gcgtattccg
     3421 cagggcgggg aagtgcgcgg taatctggcc gccggtggcc ttggcgtcac gcagccgatc
     3481 gcgccacacg agctagaaat cgccgaagcg ctggcgccag tattggcgca acgtgggctg
     3541 ttgctggtag gattggacgt gattggcagt tatttgacag aaatcaatgt caccagtccg
     3601 acctgcttcc aggaaatcac gcaacaaagc ggcttcgatg ttgccgccat gttcgtcgat
     3661 gcgctggaag ctgctgtcgc tgaacctggt ttggcaacgt aagctcatta caattcatgg
     3721 ttggtattct tcttctcaca catgctccgt tgggccaggc tttcatcacg gcggtaacgc
     3781 acgtgttttg cgatgtaccg gaacgtatgg aagccattga tgtcattgcc gatcagagtc
     3841 cgtcggaagt gcaaaagctg gcggcagatg ccgtatgccg cctcgacgat ggcagcggcg
     3901 tgctggtgat caccgacgtt ttgggtggca caccttccaa ttgctgcact tccctgtgcg
     3961 ccatcagcag
//
LOCUS       DASRDH010000230         2462 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1657065,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000230 DASRDH010000000
VERSION     DASRDH010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1657065"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            106..441
                     /gene="cyaY"
                     /locus_tag="VFA14_05340"
     CDS             106..441
                     /gene="cyaY"
                     /locus_tag="VFA14_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016833170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HZS80833.1"
                     /translation="MTESEFLVLADAALSAIEAALEQASDDTDLDVECRRSGNVLEIE
                     CVGNGSKIIVNSQAPMQELWLAAKSGGFHYQYNGRLWISARDGSELYATLSAAVTAQA
                     GVAVTLRAP"
     gene            495..1076
                     /locus_tag="VFA14_05345"
     CDS             495..1076
                     /locus_tag="VFA14_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009463333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HZS80834.1"
                     /translation="MADTAETVIKTAFAPISLQGRPFVWRIGALVLGVLLLTLSSYIK
                     VAMIPVPVTMQTLAVTLIGAIYGWRFGGLTIMAWLLQGALGMPVLAGGAAGIQHFTGP
                     TGGYLLAFPIAGALMGWLTQRGWNGNRMVFAFAGMLISNGLCLLFGATWLATNIGAEQ
                     ALMLGALPFLAGGVLKSVLGAFILKLLPWNKSA"
     gene            1058..1501
                     /locus_tag="VFA14_05350"
     CDS             1058..1501
                     /locus_tag="VFA14_05350"
                     /inference="COORDINATES: protein motif:HMM:NF018621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1284 domain-containing protein"
                     /protein_id="HZS80835.1"
                     /translation="MEQERMTIRLRPHHLLCMLTYVGKGYSSAFTTNYEVIIRRLRRG
                     EDILVVSGPDDICSPLLESVGPHCLRASVTERDRLAAHDVGKFLACRVEAGHRITPDP
                     GFLHRMREAFASRHIRNACIGCEWNELCSSVMANSHGGILIRIKE"
     gene            complement(1584..>2462)
                     /locus_tag="VFA14_05355"
     CDS             complement(1584..>2462)
                     /locus_tag="VFA14_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011353112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HZS80836.1"
                     /translation="DIPHSMLPQVRQSSEIYGHTKTTVFASKIPLAGIAGDQHAALFG
                     QMCIDPGMVKNTYGTGCFMVMNTGDQPIVSRNNLLTTIAWQVGGKLQYALEGSIFIGG
                     AVVQWLRDGLGIIRHARDIEALARSVPGSDGVYLVPAFAGLGAPYWMPHARGALFGVT
                     RGTTAAHLARAALDSIAYQTMDVLRAMQADAGLAITQLRVDGGVTTNDLLMQFQADIL
                     GVEVVRPQVTETTALGAACLAGLAVGFWSDMGEIRRQWRQDASFRPALDERQVSGLVR
                     GWQRAIAAARSWADDR"
BASE COUNT          557 a          752 c          654 g          499 t
ORIGIN      
        1 cctgccaaca gcagcaccaa cgcaatggta gatacgaccg gagcaaaaca aaatggaaac
       61 ttcacgatag aatcacggac ttatctcaat gtcccggagt gtagcatgac agaatcagaa
      121 ttcctggtcc tcgcggatgc cgcgctcagc gcgatcgaag ccgcactgga acaagcaagc
      181 gatgacactg acctcgacgt tgaatgccgc cgcagcggca acgtcctgga aatcgaatgc
      241 gtcggcaacg gctccaagat catcgtcaac agccaggcgc caatgcagga actgtggctg
      301 gcagccaaat caggcggttt ccactaccaa tacaacggca gactatggat cagcgcgcgc
      361 gacggttctg aactgtacgc cacactgtcg gcagccgtca cggcacaggc cggcgtcgcg
      421 gtgacgttac gcgcgccttg atttttattc aaattcgtag agaaccctgt ccgttgatat
      481 aaaagaaaat ccatatggct gatactgcag aaacagttat caaaaccgct ttcgcgccta
      541 tcagcctgca aggtcgacca ttcgtatggc ggatcggggc actcgtcctg ggtgtccttc
      601 ttctgacact ctcgtcatac ataaaagtag cgatgattcc agtaccggtc accatgcaaa
      661 cccttgcggt cacactgatc ggcgcaattt atggctggcg tttcggtggc ctaacaatca
      721 tggcatggct cctgcagggt gctcttggta tgcctgtgct agccggcgga gctgccggca
      781 tccagcattt caccgggccg actggcggct accttctggc ttttcccatc gcaggcgcgc
      841 tcatgggctg gctgacacaa cgaggatgga atggcaaccg tatggtgttt gcctttgccg
      901 gcatgctcat aagcaatggt ctttgtctgc tgttcggcgc tacctggctt gccacaaaca
      961 tcggagcaga acaggcattg atgctgggcg cactcccctt tctggccggc ggagttctca
     1021 aatctgtgct gggcgcgttc atactcaagc tgctgccatg gaacaagagc gcatgaccat
     1081 ccggctccga ccgcatcatc tgctctgcat gctgacctat gtcggaaaag gctacagctc
     1141 tgcatttacg accaactacg aagtgatcat aaggcggctt cgccgcggtg aagatatcct
     1201 cgtcgtttcc ggtcccgatg atatatgctc accgctcctt gagagcgtcg gaccacattg
     1261 cttgcgtgca agcgttaccg aacgcgaccg gcttgccgcc catgatgtag gcaaatttct
     1321 ggcctgccgg gtcgaagccg gccacaggat cacacccgat ccggggttct tgcatcgaat
     1381 gcgtgaagcc tttgccagca ggcacatccg caatgcctgt atcggatgtg aatggaacga
     1441 gctttgcagc tcagtgatgg caaacagcca tggcgggata ttaatccgga taaaagaata
     1501 gttttttcag aaattctgat tacgctgaac cagcggatcc ttgctgcaat aatcctgaac
     1561 gacaaattga aactgtctcc tattcatcga tcatccgccc acgacctagc agccgcaatc
     1621 gcacgctgcc agccgcgcac caacccgctt acctggcgct catccaaggc cggcctgaaa
     1681 ctggcgtcct gccgccattg gcggcgtatc tctcccatat cagaccaaaa tccgaccgcc
     1741 agtccggcca gacaggccgc accgagcgcc gtggtctccg tcacctgcgg acgcactacc
     1801 tcgacaccca gaatgtccgc ctggaactgc atcagcaaat cattggtggt gacgccgccg
     1861 tcgacgcgca gttgcgtgat ggcgagaccg gcatcagcct gcatggcccg caagacatcc
     1921 atggtctgat aagcaatgct gtcgagcgcc gcccgcgcca aatgcgccgc agtggtaccg
     1981 cgtgtcacac cgaacagggc gccacgcgca tgcggcatcc agtacggcgc gccgagtccg
     2041 gcaaaagccg gcaccagata gacgccatca ctgccgggca cgctgcgcgc cagcgcttcg
     2101 atgtcgcgcg catgacggat gataccgaga ccgtcgcgca gccattgcac caccgccccg
     2161 ccgatgaaaa tactgccctc cagcgcatat tgcaacttgc cgccgacctg ccaggcaatg
     2221 gttgtcagta gattgtttct ggaaacgatg ggttggtcac cagtgttcat caccatgaaa
     2281 caacccgtgc catacgtgtt cttgaccatg cccggatcga tgcacatctg gccgaacaaa
     2341 gccgcatgct ggtcgcccgc gataccagcc agcggaattt tcgaagcaaa caccgtggtc
     2401 ttggtatgac cgtaaatctc actggactgc cgcacttgcg gcagcatgct gtgcggaata
     2461 tc
//
LOCUS       DASRDH010000231         2642 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1570083,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000231 DASRDH010000000
VERSION     DASRDH010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1570083"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..793)
                     /locus_tag="VFA14_05360"
     CDS             complement(<1..793)
                     /locus_tag="VFA14_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016836401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CobD/CbiB family protein"
                     /protein_id="HZS80837.1"
                     /translation="MTFLSILFALLIEQLKPLRADNLIYTWIKFFAARIENWFNAGHA
                     HHGRLGWLVMVAALTVPTGMIYWVCLCISPLAALAWNVLVVYMTLGFRHYSNYFTSIQ
                     LALNTGDEVAARALLAEWTRQDVSGMDASEISRIASEHALITTHRHVFGVFFWFLMPV
                     GPACAVMYRVAEYLARAWNEPHHMKQEIFGRFAALAFYWIDWVPARFTAAAFAVVGNF
                     EDAVYAWRNFADRWTDQAVGIILAAGGGAMGVRFGTPAESAASGTL"
     gene            complement(891..1556)
                     /locus_tag="VFA14_05365"
     CDS             complement(891..1556)
                     /locus_tag="VFA14_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008327245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HZS80838.1"
                     /translation="MANFDPFLLPIDAISGEARLDAERMQEDWLRQRFVNPLPWTPER
                     SGERRIRATGAPPRPAAVLMPIVIREEGAMLLFTRRTANLRDHAGQISFPGGSTEPGD
                     ASPIDTALRETEEEIGLARSRVEIIGNLPEYFTGTGYRITPVVGLVKPPFEVAPDPGE
                     VAEIFEVPLAFLMDGMHHQRRTVASPWEDQRRTLYTMPYQHFFIWGATAGMLRNLFHF
                     LRS"
     gene            complement(1639..2016)
                     /gene="rplS"
                     /locus_tag="VFA14_05370"
     CDS             complement(1639..2016)
                     /gene="rplS"
                     /locus_tag="VFA14_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HZS80839.1"
                     /translation="MDLIQQLEQEEISRLAKNIPDFAPGDTVVVSVNVVEGTRKRAQA
                     YEGVVISRRNRGLNSNFIVRKISSGEGVERTFQLYSPLIALIEVKRRGDVRRAKLYYL
                     RERSGKSARIKEKLPHRPSTTKA"
     gene            complement(2135..>2642)
                     /locus_tag="VFA14_05375"
     CDS             complement(2135..>2642)
                     /locus_tag="VFA14_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HZS80840.1"
                     /translation="SRVVHLSPQGKPLTHQRVMHLVQEPGVVLLCGRYEAIDQRLLDS
                     HVDEEISLGDFVLSGGELPAMTLMDAVIRQLPGVLNAGSSVAEDSFVNGLLDYPHYTR
                     PEEDEGVAVPSVLIGGHHAEIEKWRRERALETTAKKRPDLIIKARRDGLLSKEDEEFL
                     SSFGSWRQ"
BASE COUNT          609 a          789 c          719 g          525 t
ORIGIN      
        1 ataaggtgcc ggatgctgcc gactccgccg gcgtaccgaa acgcacgccc atggcgccgc
       61 cgcctgctgc cagaatgatg ccgacagcct ggtctgtcca gcgatcggca aaattgcgcc
      121 aggcatagac ggcatcctca aaattaccaa ctaccgcaaa tgctgcagcg gtgaagcgtg
      181 cgggaaccca gtcgatccag taaaacgcga gcgcggcaaa acggccgaag atctcctgct
      241 tcatatgatg cggctcattc caggcgcgcg ctaggtattc ggcaacgcga tacatcacag
      301 cgcaggccgg ccccaccggc atcaggaacc agaaaaatac gccgaataca tgacggtgcg
      361 tagtgatcaa cgcgtgttcg gaggcaatac gagagatttc actggcatcc atgccggaga
      421 cgtcttgcct ggtccattcg gccagcaaag cgcgcgccgc gacttcgtcg ccggtattga
      481 gcgccagttg aatcgaagtg aaataattac tgtaatggcg aaagcccagt gtcatgtaga
      541 cgaccagcac attccaggct aatgcggcta gcggactgat acacaggcag acccagtaga
      601 tcatgccggt gggcacagtc aaggccgcta ccatgaccag ccatcccaga cggccatgat
      661 gcgcgtgacc ggcattgaac cagttctcga tgcgtgccgc aaagaatttg atccatgtat
      721 aaatgagatt gtctgcccgc agcggcttca gttgttcgat cagaagcgca aagagtatgg
      781 aaagaaatgt catcaaatcg cctttcggga aagtgcgtca atgatacaac ggtggcaagc
      841 ctcgccgacg ctgcttgccg ccgacaccca tcgcgacgaa acgccgcgtt tcaactacgc
      901 aaaaaatgaa acagattgcg cagcatccct gccgtcgcac cccagataaa aaaatgctga
      961 tacggcatgg tatacaaagt acgccgctga tcttcccagg gtgatgcaac cgtgcggcgc
     1021 tgatggtgca tgccgtccat cagaaatgcc agcggcactt cgaaaatttc ggcgacttcg
     1081 cccggatcgg gcgcgacctc gaaaggcggc ttgaccaagc ccaccaccgg tgtgatgcga
     1141 tagccggtac cggtaaagta ttccggcagg ttgccgatga tttccacacg gctgcgcgcg
     1201 agaccgattt cctcttcggt ttcacgcaag gccgtgtcga tgggcgacgc gtctcccggt
     1261 tcggtgctgc cgccaggaaa actgatctgg ccggcatgat ctctgaggtt ggcggtgcgc
     1321 cgggtaaaca acagcatcgc tccctcctcg cgtatcacga tcggcatcag caccgctgct
     1381 ggacgcggcg gcgcaccggt agcccgaatg cgccgctctc cgctgcgttc gggcgtccat
     1441 ggcagtggat tgacaaaacg ctgccgcagc cagtcttcct gcatgcgttc agcatccagc
     1501 ctcgcttcgc cggagatagc gtcgatcgga agaagaaaag gatcgaaatt cgccacggcc
     1561 atccttttga aatcgccata caaaaagggc gtccgaagac gccctttgtt catgcctgta
     1621 tcaaagacgg tgcgaagatc aggctttggt cgtactgggg cggtgaggca gtttttcctt
     1681 gatacgcgca gacttgccag aacgttcacg cagatagtac agtttggcgc gacggacatc
     1741 gccgcgacgc ttgacctcga tcaaggcgat cagtggtgag tatagctgga atgttcgctc
     1801 gacgccttcg ccggaagaaa ttttacggac gatgaagttg gagttcagac cacggttgcg
     1861 tcgcgaaatc acgacgcctt cataagcctg ggcgcgcttg cgcgtacctt cgaccacatt
     1921 gacgctgacg acgacggtat cacctggcgc aaagtcagga atattctttg ccaaacgcga
     1981 aatctcttct tgctctaatt gctggatcag atccattttt tactccggaa accatcttgc
     2041 cggcgcctgc tgcatcggtg tcgaaccgat atgcccggca gaggatgggg atttatcgac
     2101 agacacgacc aaaacggtca cgcccgcact tttgctactg ccgccaactg ccaaaactgc
     2161 tcaaaaactc ttcatcttcc ttgctcaata aaccatcccg gcgcgccttg ataatcagat
     2221 cgggacgctt tttcgcggtt gtctcgagcg cccgctcgcg ccgccacttc tcgatctctg
     2281 cgtggtgtcc gcctattagg accgatggca ccgcgacgcc ttcatcttct tctgggcggg
     2341 tgtagtgcgg ataatccagt aagccgttga caaaactgtc ttctgccacc gaactgccgg
     2401 cgttgagtac gccaggcaac tgccggatca ccgcgtccat cagcgtcatg gccggcaact
     2461 caccgccaga cagaacgaaa tcgccgaggc tgatttcttc atccacatgg ctatccagca
     2521 aacgctggtc gatcgcctca tagcggccac acaataacac gacgccaggc tcctgtacca
     2581 gatgcatgac acgctggtgc gtcagcggct tgccttgcgg cgacagatgc accacacgcg
     2641 ac
//
LOCUS       DASRDH010000232         2222 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_496425,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000232 DASRDH010000000
VERSION     DASRDH010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_496425"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..165
                     /locus_tag="VFA14_05380"
     CDS             <1..165
                     /locus_tag="VFA14_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020750069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HZS80841.1"
                     /translation="IGAIGSGGIFAQSAAKALQENTDLAPADIVRKSLVIAGELCIYT
                     NLNHIIETLD"
     gene            197..1531
                     /gene="hslU"
                     /locus_tag="VFA14_05385"
     CDS             197..1531
                     /gene="hslU"
                     /locus_tag="VFA14_05385"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013232231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HZS80842.1"
                     /translation="MHMTPKEIVSELDKHVVGQSRAKRAVAIALRNRWRRQQVAEPLR
                     HEITPKNILMIGPTGVGKTEIARRLARLADAPFIKVEATKFTEVGYVGRDVDAIIRDL
                     IDIGIKQMRESEVRKVRARAEDAAEDRVLDILLPPPRDFGFTPESAESDKSANTTRQT
                     FRKRLREGMFDDREIEIELTEVTPQMEIMAPPGMEEMTEQIKSMLSGFGNNKKSMRKT
                     KIKEAMKFLIEEEAGKLVNEEDLKQKAIANVEQNGIVFLDEIDKIATRSQGNADVSRA
                     GVQRDLLPLVEGTTVNTKHGMIKTDHILFIASGAFHLAKPSDLIPELQGRFPIRVELD
                     SLSIDDFKLILTGTDACLTRQYEALLATEGIRLEFADSGIQQLAEIAYVVNEKTENIG
                     ARRLYTVMEKMLEEISFSAGDSSEQTVSIDAAYVNARLGELAVNEDMSRYVL"
     gene            1588..>2222
                     /locus_tag="VFA14_05390"
     CDS             1588..>2222
                     /locus_tag="VFA14_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014896124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HZS80843.1"
                     /translation="MPEQYILALDQGTTSSRAVLFDHAGRVVSMAQKEFAQIYPQPGW
                     VEHDPQEIWATQAGVAAEAVTKAGLNGASIAAIGITNQRETTIVWDRASGRPLYNAIV
                     WQDRRTAALCDALKSAGHQETIRTKTGLLVDAYFSGTKIRWILDQVPGARALAQAGRL
                     AFGTIDSWLIWNFTRGELHVTDVSNASRTMLFNIHTLEWDADLLELLDIPH"
BASE COUNT          509 a          623 c          640 g          450 t
ORIGIN      
        1 attggcgcca tcggttcggg cggtatcttt gcccagtctg ctgcgaaggc gttgcaggag
       61 aacaccgatc tggctccggc cgacatcgtc aggaaatcgt tggtgattgc cggtgagctg
      121 tgtatctata ccaacctgaa ccacatcatt gagacgcttg attaagcgtc gcgtatcgta
      181 aagaatccca gccatcatgc acatgactcc taaagagatt gtttccgaac tcgacaaaca
      241 cgtcgtcggc cagagtcgcg ccaagcgtgc cgtcgccatc gccttgcgca accgctggcg
      301 tcgccagcag gtcgccgagc cgctacgcca tgagatcaca ccgaagaaca tcctgatgat
      361 cggccccacc ggcgtcggca agacagaaat cgcccgccgt ctggccaggc tggccgatgc
      421 gccgtttatc aaggtcgaag cgaccaaatt cactgaagtc ggttacgtcg gccgcgatgt
      481 cgatgccatc attcgtgacc tgatcgacat tggcatcaag caaatgcgtg aatccgaagt
      541 gcgcaaggtc cgcgcgcgcg cagaagacgc tgccgaagat cgtgtgctcg acatcctgct
      601 gccgccgccg cgtgatttcg gtttcacgcc ggaaagcgcg gaaagcgaca agagtgccaa
      661 taccacgcgt cagactttcc gcaaacgtct gcgcgagggt atgttcgacg atcgtgagat
      721 cgagatcgag ttgaccgagg tcacgccgca gatggaaatc atggcgccgc cgggcatgga
      781 agaaatgacc gaacagatca agtccatgct ttccggtttc ggtaataaca agaagagtat
      841 gcgcaagacc aagatcaagg aagccatgaa gttcctgatc gaagaagaag ccggcaagct
      901 ggtcaatgaa gaagatctga agcagaaggc gattgccaat gtggaacaga acggtatcgt
      961 tttccttgat gagatcgaca agatcgccac gcgttcgcaa ggtaacgccg acgtttcgcg
     1021 cgctggtgtg cagcgtgatc tgttgccgct ggtcgaaggt acgaccgtca acaccaagca
     1081 tggcatgatc aaaaccgacc acatcctgtt catcgcatcg ggggctttcc atctggccaa
     1141 gccgtccgat ctgattcctg aactgcaagg tcgctttcct atccgggtcg aactcgattc
     1201 cctgtcgatc gatgacttca agctcatcct aaccggaacc gatgcctgtc tgaccaggca
     1261 gtatgaagcc ttgctcgcta ccgaaggcat tcggctcgag tttgccgaca gcggcataca
     1321 gcagctggcc gagatcgcct atgtggtcaa tgaaaaaacg gaaaacatcg gtgcgcgccg
     1381 tttgtacacc gtgatggaaa aaatgctgga agaaatttct ttctctgccg gtgacagtag
     1441 cgagcaaacc gtcagcatcg atgcggccta tgtcaatgcg cgtctgggcg aactggcagt
     1501 caatgaagac atgtcgcggt atgtgctgta atgatggcag ctgccatcgt tgcatggcat
     1561 cgttgactat cgccgacgga ggaagacatg ccggagcagt acatcctggc gctcgatcag
     1621 ggcacgacca gttcgcgtgc cgttctgttc gatcatgctg gacgggtcgt cagcatggcg
     1681 cagaaggaat tcgcgcagat ttatccgcag cccggctggg tggagcatga tccgcaggaa
     1741 atctgggcca cccaggctgg cgtagccgcc gaagcggtca ccaaagccgg tctgaatggc
     1801 gcctcgattg ccgccatcgg tatcactaac cagcgcgaga ccaccatcgt ctgggatcgt
     1861 gccagtggtc gcccgctata caacgccatc gtctggcagg accgccgaac ggctgcgttg
     1921 tgcgatgcgc tgaaaagcgc aggccatcag gagaccatcc gcaccaagac cggcctgctg
     1981 gttgatgctt atttttccgg cactaaaatt cgctggatcc tcgaccaggt acctggcgcg
     2041 cgcgctctgg ctcaggctgg gcggctggca ttcggcacca tcgatagctg gctgatctgg
     2101 aatttcacgc gtggggaact gcatgtcact gatgtcagca atgcctcgcg caccatgctt
     2161 tttaatatac acacgctgga atgggatgcg gatctgctgg agctgctgga tattccgcac
     2221 ag
//
LOCUS       DASRDH010000233         2619 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_657442,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000233 DASRDH010000000
VERSION     DASRDH010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_657442"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1514
                     /locus_tag="VFA14_05395"
     CDS             <1..1514
                     /locus_tag="VFA14_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HZS80844.1"
                     /translation="ALKVFCHLHALSLRFHLQRQTGGMTRDIERGTRGVSSLVSYTLF
                     SILPTLIEIALVLAYLAWHYEIWFSIITAGALAVYIVFTITVTEWRTHFRRTMNELDS
                     RANAKAIDSLLNYETVKYFGNEDFEARRYDEGLLRYEAAAIKSQSSLSLLNSGQSAII
                     AIATTLILWRATQGVIDGRMSLGDLVLVNAFMIQLYIPLNFLGVIYREIKQSLADMER
                     LFSLLEQNREVADAPDAVPLHTDGAAVRFAHVDFSYERKRQILFDVDFELVAGTTTAV
                     VGHSGSGKSTLSRLLYRLYDVDGGSITIDGQNVREVTQASLRRAIGIVPQDAVLFNDT
                     IEYNIAYGKPGSSREEIVAAAKAAYVHDFIASLPDGYATTVGERGLKLSGGEKQRVAI
                     ARTLLKNPAVLVFDEATSALDSKAEQAIQAQLREIARNRTTLIIAHRLSTIVDAYQVL
                     VLEYGRIIERGTHMQLLAAGGAYAQMWARQQARDDDRTDEGESDPVVRALRFL"
     gene            1633..>2619
                     /locus_tag="VFA14_05400"
     CDS             1633..>2619
                     /locus_tag="VFA14_05400"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HZS80845.1"
                     /translation="MSNITVKHEIPSYLTPHGVGPWDVYLEQIERVTPYLGTLARWVE
                     TLKRPKRMLIVDVPIERDDGTIAHFEGYRVQHNTSRGPGKGGVRFHQDVTLSEVMALS
                     AWMTVKNAAVNVPYGGAKGGIRIDPKTLSCGELQRVTRRYTSEIGIIIGPNKDIPAPD
                     VNTDAQIMAWMMDTYSMNQGSTASGVVTGKPISLGGSLGRHEATGRGVFVVGCEAAAK
                     CHLEISNARVAVQGFGNVGGIAARLFAEAGAKIVAVQDHAATVVRASGLDIPALRGHV
                     AQYGSVAGFPGGDEVADRVQFWASDCDILVPAALEQQIDASNASSVRAKIILE"
BASE COUNT          530 a          719 c          822 g          548 t
ORIGIN      
        1 tcgcgttgaa ggtgttttgc catttgcatg cgttgtcact gcgctttcat ttgcagcggc
       61 agaccggtgg catgacgcgc gacatcgagc gcggcacgcg tggcgtgtct tccctggtgt
      121 cgtataccct gttcagcatc ctgccgacgc tgatcgagat cgcattggtg ctggcgtacc
      181 tggcgtggca ctatgaaata tggttcagca tcatcaccgc aggcgcgctg gcggtgtaca
      241 ttgttttcac catcaccgtc acagagtggc gcacgcactt tcggcgcacg atgaacgagc
      301 ttgattcgcg cgcgaatgcg aaagcgatcg actccttgct caattatgag acagtcaagt
      361 atttcggcaa tgaggatttc gaagcgcgtc gctatgacga ggggttgctg cgctacgaag
      421 cggcagcgat caaatcgcaa tcctcgttgt cgctgctgaa ttcgggacag tcagccatca
      481 ttgcgattgc taccacgctg attctgtggc gcgccacaca gggcgtgatc gacggcagga
      541 tgagcctggg agatctggtg ctggtgaatg cgttcatgat acagctctac attccgctta
      601 attttctcgg cgtgatttat cgcgagatca agcaaagcct ggctgacatg gaacgcttgt
      661 tctcgttgct ggaacagaac agggaagtcg ccgatgcgcc cgatgccgta ccgttgcaca
      721 ccgatggtgc agcagtgcgg tttgcgcacg tcgatttcag ttatgagcgc aagcggcaga
      781 ttctgttcga tgtcgatttc gagcttgtcg ctgggacgac tacggcggtg gtcggccata
      841 gcggttccgg caaatcgacc ttgtcgcggc tgctgtatcg cctgtatgac gtcgatggcg
      901 gcagcatcac catcgacggt cagaatgtgc gcgaggtgac gcaagcgtcg ctacgccggg
      961 ccatcggcat cgtgccacag gatgcggtac tgttcaacga caccatcgaa tacaacatcg
     1021 cttacggcaa acctggcagt agcagggaag aaatcgtcgc agcagccaag gccgcctatg
     1081 tgcatgattt cattgcatcg ttgccggacg gctatgcgac cacggtaggc gagcgcggtc
     1141 tgaaactgtc cggcggcgag aagcagcgcg tggcgattgc gcgtactttg ctgaaaaatc
     1201 cagccgtgct ggttttcgac gaggcgactt cggcgctcga ctccaaagcg gaacaggcga
     1261 tacaggcgca gctgagagag attgcgcgca atcgcaccac actgatcatc gcgcatcgcc
     1321 tgtcgaccat cgtcgatgcg tatcaggttc tggtgctgga atatggccgc atcatcgagc
     1381 gtggtacgca catgcaattg ctggctgctg gcggcgcgta tgcgcagatg tgggcaagac
     1441 agcaagccag ggatgatgac cggacagatg agggtgagag cgatccggta gtgcgcgctc
     1501 tcaggttctt atgataaagt tgttgtcgat ttgcacggcc atatcgactt cggaaggggc
     1561 gagatgatcc atccgtgttc cgcgctcgca aagctactgt gaacaggccc tgacacaagg
     1621 aatgatcgca tcatgtcgaa cataacagtc aagcacgaaa ttccttccta tctcacgccg
     1681 catggcgtcg gtccatggga cgtctatctc gaacagatcg agcgcgtcac cccctacctc
     1741 ggcacgctgg cgcgctgggt ggaaaccctg aagcgtccca agcgcatgtt gatcgtcgat
     1801 gtgccgatcg agcgtgatga cggcacgatt gctcacttcg aaggctaccg cgtgcagcac
     1861 aatacatcgc gtggtccggg caagggcggc gtgcgcttcc atcaggacgt gacgctgtcc
     1921 gaggtgatgg cgctgtcggc ctggatgacg gtcaagaatg cagcggtgaa tgtaccctat
     1981 ggcggcgcca agggcggcat tcgcatcgat ccgaagacct tgtcgtgcgg cgaactgcaa
     2041 cgcgtgacgc ggcgctacac cagcgagatc ggcatcatca tcggaccgaa caaggatatt
     2101 cccgcaccgg acgtcaacac cgatgcgcag atcatggcct ggatgatgga tacgtactcc
     2161 atgaatcagg gcagtactgc gtcgggcgtc gtgacgggca agccgatctc gctgggcggc
     2221 agtctcggtc gtcatgaagc caccggccgc ggcgtgttcg tagtcggttg cgaagctgcc
     2281 gccaaatgcc atctggagat cagcaatgcg cgcgttgcgg tacagggctt cggtaatgtc
     2341 ggcggcattg ccgcgcgcct gtttgccgaa gccggcgcaa aaattgtggc ggtgcaggat
     2401 cacgcagcga cggtcgtgcg cgccagcggc cttgacattc ctgcgttgcg gggtcatgtt
     2461 gcgcaatatg gcagcgtcgc cggcttcccc ggcggggatg aagtggcgga ccgcgtccag
     2521 ttctgggcga gcgactgcga cattctggtg cctgccgcgc ttgagcagca aatcgacgcg
     2581 agcaatgctt cgtcggtgcg cgctaaaatc attctggaa
//
LOCUS       DASRDH010000234         4601 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_498711,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000234 DASRDH010000000
VERSION     DASRDH010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 4601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4601
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498711"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..983
                     /gene="sucC"
                     /locus_tag="VFA14_05405"
     CDS             <1..983
                     /gene="sucC"
                     /locus_tag="VFA14_05405"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006486694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HZS80846.1"
                     /translation="ARSLEQVGEYASQILGMQLVTHQTGPEGQKVRRLLIEEGADIKK
                     ELYVSMVTDRVSQRVVLMASSEGGMDIEEVAKSHPERIHRIAIDPATGLTDADADSIA
                     ARIGVPAASVADARKQLQGLYQAYWETDASLAEINPLILTGDGKVVALDAKFNFDANA
                     LFRHPEIVAYRDLDEEDPAEIEASRFDLAYISLEGNIGCLVNGAGLAMATMDSIKLFG
                     GEPANFLDVGGGATTEQVTEAFALMMRNPGLKAILVNIFGGIMRCDVIAEGVIAASKA
                     VSLNVPLVVRMKGTNEEIGKKLLADSGLSIISADSMEEAARKVVAAANGK"
     gene            1001..1885
                     /gene="sucD"
                     /locus_tag="VFA14_05410"
     CDS             1001..1885
                     /gene="sucD"
                     /locus_tag="VFA14_05410"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006218345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HZS80847.1"
                     /translation="MSILINRDTKVITQGITGKTGQFHTRMCREYANGRNAFVAGVNP
                     KKADEEFEGIPIFAHVAQAREATGANVSVIYVPPAGAAAAIWEAVEAGLDLVICITEG
                     IPVRDMLVLKDRMTKAGSKTLLLGPNCPGLITPDEIKIGIMPGHIHKKGRIGVVSRSG
                     TLTYEAVAQLTALGLGQSSAVGIGGDPINGLKHIDIMKAFNDDPDTDAVIMIGEIGGP
                     DEANAAYWIKDNMKKPVVGFIAGVTAPPGKRMGHAGALISGGADTAAAKLAIMEECGI
                     RITRNPSEMGRLLKAVLF"
     gene            1945..2550
                     /locus_tag="VFA14_05415"
     CDS             1945..2550
                     /locus_tag="VFA14_05415"
                     /inference="COORDINATES: protein motif:HMM:NF012342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HZS80848.1"
                     /translation="MKDTQTLHWRISSMRGSDAAEEKIPAIARRSGSVPNKSFFITGK
                     HSMQAARSARRAQHGFTLIELMIVIAIVGVLAMVAVPQYRDYLIRAKVVEGLDLAAPA
                     KMAVSEMIAAKGSTAFDRRMTGYSFTPSDKLKTVRDIAIGDGGIITITFGEIGGDGND
                     KTLILAPSVNASAIFWTCHAAGAASHPGSLPAQYAPENCRS"
     gene            2653..3690
                     /locus_tag="VFA14_05420"
     CDS             2653..3690
                     /locus_tag="VFA14_05420"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZS80849.1"
                     /translation="MSKKITHIRLDNFTSFAKLDQPFSIGVNVIIGANGTGKTHLLKV
                     LYAACAITVGENKEKGFALKLRNVFNPYEGRIGRLARRQSTSVKTRAIVTREGGVKLS
                     AEFSNHTNKPDDVKVGGETVWKKDELDSAYIPVKEMLAHAPGFLATAAKREIAFEEVY
                     EDIINRAFLPKLKGPIDKNRQRLLTALQKAIDGKVVAKGEYFFLKNKQGDLEFTLLAE
                     GMRKLALIWLLIQNGTLLSGSVLFWDEPEANLNPGLMGEVVDVILELHRLGVQVFLTT
                     HNYVLLKEFDLRKQKDDQIRYLSLFRDESEAVTAKASDSYSGVDPNAIAATFSDLYDR
                     ELKRSLGGIKL"
     gene            complement(3645..4268)
                     /locus_tag="VFA14_05425"
     CDS             complement(3645..4268)
                     /locus_tag="VFA14_05425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS80850.1"
                     /translation="MRQGDACRVIHSTSRQYNILQFLFHGATRLARMSGIALFHAARF
                     ITAQPATKGFFQGRSPGPAAGICRFKASVRAKSSAPESELAPNQHQVIDGFLGPTFCH
                     LIKPRITIFGFQVTINGTAQKFSDEFPAIFLISIQILGIFEFNEPNPIIKLHDEVYGF
                     HAMAQAVAGPIIELPAINTYRQSDLQIIFDKHRHSLMPPRLRLSSRS"
     gene            complement(4310..>4601)
                     /gene="rho"
                     /locus_tag="VFA14_05430"
     CDS             complement(4310..>4601)
                     /gene="rho"
                     /locus_tag="VFA14_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524120.1"
                     /note="An RNA-DNA helicase that actively releases nascent
                     mRNAs from paused transcription complexes; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HZS80851.1"
                     /translation="SRMDDVIYEEFKGTGNMEVHLERRLAEKRVYPAINLSKSGTRRE
                     ELLIKSDQLQKIWVLRKLMYDMDEIEAMEFILDKMKSTKNNAEFFDMMRRGS"
BASE COUNT         1079 a         1162 c         1328 g         1032 t
ORIGIN      
        1 ttgccagatc cttggagcaa gtcggggaat acgcgagcca gatccttggc atgcagctgg
       61 tcacgcatca aactggccct gaaggccaaa aggtgcgccg tctgctgatc gaagaaggcg
      121 cggacatcaa gaaggaacta tacgtttcca tggtcactga ccgtgtaagt cagcgcgtgg
      181 ttctgatggc ttccagcgaa ggtggtatgg atatcgaaga agttgccaaa agccatccag
      241 aacggattca tcgcatcgct atcgatccgg ccactggcct gaccgacgca gacgccgaca
      301 gtatcgccgc caggattggt gttccagccg ccagcgttgc cgacgcgcgc aagcaactgc
      361 aaggtctgta ccaggcatat tgggaaaccg atgcgtcgct ggctgaaatc aacccgctga
      421 tcctgaccgg tgacggcaag gtcgtcgcgc tggatgctaa attcaatttc gacgccaatg
      481 cattgtttcg tcatcctgaa atcgtcgctt accgcgatct ggatgaagaa gatccggcgg
      541 aaatcgaagc ttccaggttt gatctggcct acatttccct ggagggcaat atcggctgtc
      601 tggtcaacgg cgccggtctg gccatggcga ccatggacag catcaagctg ttcggcggcg
      661 agcctgccaa cttcctggac gttggtggcg gcgcgaccac cgagcaggta accgaagcat
      721 tcgcgttgat gatgaggaac ccggggttga aagccattct ggtcaacatt ttcggcggca
      781 tcatgcgctg tgacgtgatc gctgaaggtg tcatcgctgc gtccaaagcc gtttccctga
      841 acgtgccgct ggttgtgcgc atgaagggca ccaatgaaga gatcggcaag aaattattgg
      901 cggattccgg cctgtctatc atttcggcag acagcatgga agaggctgcg cgaaaagttg
      961 tcgctgccgc caacggtaaa taacaggata aggaagaacc atgtcgattc tgatcaatcg
     1021 ggacactaaa gtcattaccc agggtatcac cggtaaaacc ggtcaattcc acacacgtat
     1081 gtgccgggag tatgcgaacg gtaggaacgc cttcgttgcc ggcgtgaacc cgaaaaaagc
     1141 tgatgaagaa ttcgaaggca ttcccatttt cgcgcatgtt gcccaagcca gggaggcaac
     1201 cggcgccaat gtttctgtga tctatgtgcc tccggcgggc gcagctgctg ctatctggga
     1261 agctgtcgaa gccggactgg atctggtcat ctgtatcacg gaaggcattc cggtgcgcga
     1321 catgctggtg ttgaaggacc gcatgaccaa ggccggcagc aagacattgc tgctgggtcc
     1381 caactgcccg ggtttgatca caccggacga aatcaagatt ggcatcatgc cgggccacat
     1441 ccataaaaaa ggccgcatcg gcgtggtatc ccgttccggc acgctgacct acgaggcagt
     1501 cgcgcaactg accgcgctgg gcctgggcca gtcttccgcg gtcggcatcg gcggtgaccc
     1561 gatcaacggt ctgaagcata tcgacatcat gaaggcgttc aatgatgatc ctgacaccga
     1621 tgcagtgatc atgatcggtg aaatcggcgg tcctgacgaa gccaatgctg cgtactggat
     1681 caaggacaat atgaagaagc cggtggttgg tttcatcgct ggtgtaactg cgcctccagg
     1741 taaacgcatg ggccatgccg gggcgttgat ttccggtggc gccgacacag cggcggccaa
     1801 gctggcgatc atggaagaat gcggtatcag aatcacgcgc aatccatctg agatgggtcg
     1861 cctgttgaag gctgttctgt tttaagagcg gagtgccgga aattctgcat acgcccacac
     1921 agaatttctg gatggcccgg cgacatgaaa gatacacaaa cgctgcattg gcgcatatca
     1981 tccatgcggg gaagcgacgc tgccgaagag aaaatcccgg ccattgcgcg acggtcaggc
     2041 agcgttccca ataaatcgtt ttttataaca ggaaaacact ctatgcaagc agcacgttcg
     2101 gctcgccgcg cacaacacgg tttcaccttg attgaactga tgatcgtcat cgccattgtc
     2161 ggtgttctgg ctatggtagc ggttcctcag tatcgggact acctgattcg ggcaaaagtg
     2221 gtcgaggggc tggatctggc ggcgccggcc aagatggcag tatcggagat gattgccgcg
     2281 aagggcagta cagcgtttga tcggcgtatg accggctatt ccttcacgcc gtccgacaag
     2341 cttaaaacgg ttagagatat tgcgattgga gatggtggca tcatcaccat tacctttggc
     2401 gagatcggcg gcgatggcaa cgacaagacc ttgattctgg cgccgtccgt caatgccagc
     2461 gcgattttct ggacctgcca tgcggctggt gcggcaagtc atccaggctc tttgccagcg
     2521 caatatgctc cggaaaactg ccgcagctga tctgagcttt cctcagaatt tcgccttcag
     2581 aaaaatctgg ttgcataaag aatacctctt ggaaaacgaa gaaatgaggg aacctcaaac
     2641 ggaggacggg ttatgagcaa gaagatcacg cacataaggc tggacaattt cacatcattt
     2701 gccaagttgg atcagccctt ctcaattggt gtgaatgtca tcattggcgc caatggcacc
     2761 ggcaagacac acttgctcaa agtgctttat gcagcctgtg caataacagt gggcgagaat
     2821 aaggagaaag gttttgcgct caagctgcgc aacgtattca atccctatga ggggcgaatc
     2881 gggcgtctcg cgcgaaggca gtcgaccagc gtcaaaacca gggctatcgt cacccgcgaa
     2941 ggtggcgtga agctgtcggc ggaattttcc aatcacacca acaaacctga tgatgtgaag
     3001 gtggggggcg agacagtctg gaaaaaagat gagctggaca gcgcctatat cccggtcaag
     3061 gagatgttgg cccatgctcc tggttttttg gccactgccg ccaaacgcga aatcgccttt
     3121 gaagaagtct atgaggacat tatcaaccgg gccttcctgc ctaagttgaa ggggccgatc
     3181 gataagaatc gtcagcgctt gctgacagct ctacagaagg ccatcgatgg caaggttgtc
     3241 gccaagggcg agtacttttt cctcaagaac aaacaaggcg atctggaatt caccctgctt
     3301 gccgagggta tgcgtaagct ggccttgatt tggctgctga ttcagaacgg cacattgctt
     3361 tccggttcgg tgctgttttg ggatgaaccc gaagctaacc tgaatccagg cttgatgggt
     3421 gaggtggtcg acgtcattct ggaattgcat cgcctgggtg tgcaggtgtt tttgacgacg
     3481 cacaattatg tgctgctcaa agaattcgat ctgcgcaagc agaaagacga tcagattcgc
     3541 tatctgtcac tgttccgtga tgaatcagaa gcggtgactg ccaaggcgag tgatagttat
     3601 tctggtgttg atcccaatgc gattgccgcc acgtttagcg acctctatga ccgcgaactc
     3661 aagcgcagcc tgggaggcat caaactatga cggtgcttgt cgaagatgat ctgcaaatca
     3721 ctctgccggt aggtgttgat ggccggaagt tcgatgatgg ggccagccac ggcttgagcc
     3781 attgcatgaa agccgtagac ttcatcgtgg agtttaatga taggatttgg ttcgttgaat
     3841 tcaaagatcc cgagaatctg aatgctaatc aaaaagatag cggggaattc atcagaaaat
     3901 ttctgagcgg tgccattgat agtgacctga aaaccaaata tcgtgattcg tggctttatg
     3961 agatggcaga aggtcgggcc aagaaaccca tctattacct ggtgttgatt gggtgccagt
     4021 tcgctttccg gcgcggagct tttggctcgc acggatgcct tgaagcgaca aatccccgca
     4081 gcaggcccag gtgaccggcc ctggaagaaa cctttcgtag ctggttgtgc cgtgatgaat
     4141 cttgccgcat ggaatagcgc tattcctgac attctggcaa gccgggtcgc tccgtgaaac
     4201 aggaattgca gaatattgta ttggcgactt gtactgtgga tcacccggca ggcatcgcct
     4261 tgccgcatga ttcagcggca ggacgtgtgg ggtgcctagc gccgccagct taactaccgc
     4321 ggcgcatcat gtcgaagaac tctgcattgt tcttggtcga cttcattttg tcgaggatga
     4381 attccatcgc ctcgatttcg tccatgtcgt acatcagttt gcgcagcacc cagatcttct
     4441 ggagctggtc tgacttgatc agcagctcct cgcggcgggt gccagacttg ctgaggttga
     4501 tggccgggta gacacgcttc tcggcgaggc ggcgttccag gtgaacttcc atgttgccgg
     4561 tacccttgaa ttcttcatag atcacgtcat ccatgcggct g
//
LOCUS       DASRDH010000235         6392 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_959561,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000235 DASRDH010000000
VERSION     DASRDH010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 6392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6392
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_959561"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1022)
                     /gene="radA"
                     /locus_tag="VFA14_05435"
     CDS             complement(<1..1022)
                     /gene="radA"
                     /locus_tag="VFA14_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HZS80852.1"
                     /translation="MAKTRTGYACTECGGISNKWAGRCLACGQWNTLMETLIETGGTH
                     RYSSQHQGLAHTAPILSLVDIEAIDVPRFGTGIAEFDRVLGGGLVPGGVVLIGGDPGI
                     GKSTLLLQALAHLSRCKQVLYVSGEESGSQIALRAKRLALDAGSLKLQAEIQLEKILG
                     SLAGHQPDVAVIDSIQTIYSDALTSAPGSVAQVRECAAQLTRAAKTADTTIIMVGHVT
                     KEGALAGPRVLEHIVDTVLYFEGDTHSSFRLVRAFKNRFGAVNELGVFAMTEKGLKGV
                     ANPSALFLSQHDRQVPGSCVMVTQEGTRPLLVEIQALVDHSHVPNARRLSVGLEQNRL
                     AMLLAVL"
     gene            complement(1024..2103)
                     /gene="alr"
                     /locus_tag="VFA14_05440"
     CDS             complement(1024..2103)
                     /gene="alr"
                     /locus_tag="VFA14_05440"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005669714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HZS80853.1"
                     /translation="MPRPIVATIDMSALRHNLAIARSHAPHARVWAVVKAQAYGHGLD
                     RAVRAFADADGLALVEVDYAAHLRRLGWQKPILLLEGFFDAGDIAVMAEQQLQCVVHC
                     HEQIRMLENIGVEAQFDVHLKMNSGMNRLGFKPEAYRAAYTRLSRLACVRSMTCMTHF
                     ANADDEGNPHLSLAEQIRRFKAATTGLAGARSVSNSAVDLLHEELAFDWVRPGVMLYG
                     GTPGTRAAAEFGLRPAMTLESRIIGVQDIGSGDAVGYGSRFVADRPMKIGVVACGYAD
                     GYPRQAPDATPVMVNGVRTGTVGRVSMDMLAVDLTPVPRAGIGSRVILWGESLPIDEV
                     AAAAGTTGYELMCALAPRVTVREMG"
     gene            2422..3687
                     /gene="lplT"
                     /locus_tag="VFA14_05445"
     CDS             2422..3687
                     /gene="lplT"
                     /locus_tag="VFA14_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008119424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HZS80854.1"
                     /translation="MNRGFYTIMAAQFFSSLADNALLITAIALLTEMQSPAWMTPLLK
                     LVFVLSYVLLAAFVGAFADAFPKGRVMFITNLIKISGCALMFFTLHPLTAYAIVGLGA
                     AAYSPAKYGILTELLPPEKLVIANGWIEGLTVGSIILGTVLGGALLNPHVTSALLSFD
                     VPLLDLNIDTPAKAALLVIILVYIIAAVFNLRIPDTGARYAHQQRNPIKLIADFSRCF
                     IVLWKDRLGQISLAVTTLFWGAGATLQFIVLQWAEKSLHMPLDRAAILQGIVALGVAV
                     GAVGAARFVPLKKSLTVMPLGIIMGLIVMVMTTVTSIWIAYPLLIVIGVLSGYFVVPM
                     NALLQHRGHVLMSAGHSIAVQNFNENLSVLIMLAFYAMLVMLDLDVNIVIIIFGLLVA
                     STMYLIMRRHAANQRDRDSLALIGEHKQH"
     gene            complement(3717..4535)
                     /locus_tag="VFA14_05450"
     CDS             complement(3717..4535)
                     /locus_tag="VFA14_05450"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HZS80855.1"
                     /translation="MSQQPLAASVRILEEIGIGPVWVRRQPAVDTTQTDAAAVPPQTM
                     SAAGRLAIPDPVAPVSQATSAQEDSAAMVGIPSAIATMNWDRLKETVASCRQCSLCRG
                     RKKTAFGVGDEKAKWLFIGEGPGRNEDIQGEPFVGAAGKLLDNMLVAMGLKRGDNAYI
                     ANIVKCRPTDDKGKDRPPSPQETASCMPYLQRQIDLIQPTILVALGKTAALSLLGLDP
                     ATPVSKLRGTVHRYRNWPLIVTYHPAYLLRQPGDKRKSWADLCLAMTAYADATH"
     gene            complement(4532..5023)
                     /gene="rimI"
                     /locus_tag="VFA14_05455"
     CDS             complement(4532..5023)
                     /gene="rimI"
                     /locus_tag="VFA14_05455"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HZS80856.1"
                     /translation="MSLDALLRELESRFGKLAFAAMQMADLDELMLIEDRIYTRPWTC
                     GNFQDALRSGYQALTMRDAAGRLLAYFLMMEAMDEAHLLNLSVAEEVQGEGFGHLLLR
                     HAVDLARRRQMKIMLLEVRVSNLRALHVYRRHGFVEIGRRKNYYPVDQHTREDAIVMS
                     LPL"
     gene            complement(5020..5724)
                     /gene="tsaB"
                     /locus_tag="VFA14_05460"
     CDS             complement(5020..5724)
                     /gene="tsaB"
                     /locus_tag="VFA14_05460"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HZS80857.1"
                     /translation="MSIILAIETSTELASAALLHGDALIVRESMGVQMHAASILPMVQ
                     QLLAQAGIALSQCDAIALGIGPGSFTGVRTACGVAQGLAFGSDLPVVPVVTLEAMAQA
                     CLETVGSEEVLAILDARMGEVYWAQYRHGTHGWQPVITPTLSAAHDVLPQGAAQACGN
                     GLTVYAQDVAMQTFAAAGLEQVMPHAAQVATLGHRAFAQGNMVSPQAAQPLYVRNKVA
                     LTTAERQVRDAVKGAG"
     gene            5781..6221
                     /locus_tag="VFA14_05465"
     CDS             5781..6221
                     /locus_tag="VFA14_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HZS80858.1"
                     /translation="MSYQIFSSRNREELAAALRSDKWIVACLCASWCDSCRDYAVHFA
                     AWAERHPQHHFVWIDIEDQADLVGDLDIDSFPTLLMQHGATISFFGTMEPDIRQAERL
                     LQTLSSRSAADLGREAHSTKEHRIWQEYDLRQRLDALPDAAGAA"
BASE COUNT         1336 a         2031 c         1698 g         1327 t
ORIGIN      
        1 agcactgcca acaacatagc cagccggttc tgctccagac caaccgaaag gcgacgcgca
       61 ttgggcacgt gggaatgatc gaccagggcc tggatttcca ccagcaaagg acgcgtgcct
      121 tcctgtgtca ccatgacaca agagccgggc acctgcctgt catgctgcga cagaaacagt
      181 gccgagggat tggcaacacc tttcagtccc ttttccgtca tcgcgaacac acccagctca
      241 ttgaccgcgc cgaagcgatt cttgaatgcc cgcaccagac ggaaactgga atgcgtgtcg
      301 ccttcgaaat acagcaccgt gtcgacgata tgctccagta cgcgcggccc cgccagcgca
      361 ccctccttgg tgacgtggcc gaccatgatg atggtggtat cggcagtctt ggccgcacgc
      421 gtgagctgcg ccgcgcattc acgcacctga gccacggaac ctggcgcaga cgtcaatgca
      481 tcggaataga tcgtctggat cgaatcgatc accgccacat caggctgatg tccagccaag
      541 gagccgagaa ttttttccag ttgaatctcg gcctgcaatt tgagactccc agcatcgagt
      601 gccaggcgct tcgcgcgcaa agcgatctgc gagccagact cttcgccgct cacatacagc
      661 acctgcttgc atctggagag atgcgccaac gcctgcagca acagcgtcga cttgccgatt
      721 cccgggtcac cgccgatcag caccacgccg cccggcacca gaccgccgcc gagtacgcga
      781 tcaaactctg caatcccggt accgaaacgc ggcacgtcga tcgcctcgat atcgactagg
      841 gacaggatcg gcgccgtgtg cgccagcccc tgatgctgac tggaataacg atgcgtcccg
      901 ccggtttcga tcagtgtttc catcaatgta ttccactgac cgcaggccag gcatcgtccc
      961 gcccatttat tgctgatgcc gccgcattcg gtacaggcgt aaccggttct ggtctttgcc
     1021 atctcaaccc atttcccgga cggtcacacg cggcgccagg gcgcacatca gttcgtagcc
     1081 tgtcgtgccg gcagcagcag ccacttcgtc gatcggcaag ctctcccccc acaggatcac
     1141 ccggctaccg ataccggcgc gcgggacagg cgtgaggtct accgccagca tgtccatcga
     1201 cacacgcccg accgtgccag tccgaacgcc attgaccatg acaggcgtag catcgggcgc
     1261 ctggcgcgga taaccgtcgg cataaccgca ggcaaccacg ccgatcttca tcggcctgtc
     1321 cgcgacgaaa cggctgccat aaccaacagc gtcgccggag ccgatgtcct ggacaccaat
     1381 gatccggctc tccagcgtca tcgccggacg caggccgaac tccgccgcag cgcgagtacc
     1441 gggcgtgccg ccatacagca tcacaccggg acgcacccaa tcgaacgcca gttcctcatg
     1501 cagcaggtca acagccgagt tggacacgct gcgcgcgcct gccaatcctg tggtggcggc
     1561 tttgaagcgc ctgatctgct ccgccagcga cagatgcgga ttaccctcat catcggcatt
     1621 ggcaaagtgc gtcatgcaag tcatgctgcg tacgcaggcc agtctgctca accgtgtata
     1681 cgcggcacga taggcttccg gtttgaaacc caggcgattc ataccgctgt tcatttttaa
     1741 gtgaacatcg aactgcgcct cgaccccgat gttctccaac atcctgatct gctcatggca
     1801 atgcacgaca cactgcaact gctgctcggc catgacggca atatcgccgg catcaaaaaa
     1861 accttccagc agcaagatcg gcttttgcca gccaagccgg cgcaggtgcg cggcataatc
     1921 gacttccacc agcgccagtc catcggcatc tgcaaaagcg cgcaccgcac ggtccaggcc
     1981 atgtccatac gcctgcgcct tgacgacggc ccataccctg gcgtgcggcg catgtgatct
     2041 ggcgattgcg aggttgtgcc gcagcgccga catgtcgatg gtggcaacga tgggtctagg
     2101 catacaggct tagtgacaga ataaatatgg tgaaaaccgc tgcttcaaaa acgacaccca
     2161 ttttaccggc tcttggggcg tgtcatggac tatttcatag gtcacgcagc cccgggaacg
     2221 gtctgcatgc aaggcgcaac gacgcgacgt agcagtgcta cgcaaggagt cgcaacacga
     2281 caggcggccc gctgttcccg gagctgcctg tagggttcag tcaggaacta taaaatagtc
     2341 catgacacgc tctatcgcta ccgccctgtc ttttcatggt ataaagcaag ccggagataa
     2401 tttgtcacat cttggatacg gatgaatcgc ggcttttaca ccatcatggc ggcgcagttt
     2461 ttttcgtcgc ttgccgacaa tgccctactg atcacagcta ttgcactgct tacagaaatg
     2521 caatctccgg catggatgac gccgctgctg aaattagtct ttgttctgtc ttatgtgctg
     2581 ttggccgcct tcgtcggcgc ttttgcggac gcttttccta aaggcagggt gatgttcatc
     2641 accaacctga tcaagataag cggctgcgca ctgatgttct ttacgctcca ccccctgacc
     2701 gcctatgcca tcgtgggcct cggcgccgcg gcgtattcgc cggccaaata cggcatcctg
     2761 acggaactgc tgccgccgga gaaactggtc atcgccaatg gctggatcga ggggttgaca
     2821 gtcggctcca tcatcctcgg tacggtgctg ggcggcgcat tgctcaatcc gcatgtcacc
     2881 tcggcattat tgtcattcga tgtaccactg ctcgacctga acatcgatac accggccaaa
     2941 gcagcgctcc tcgtcatcat cctcgtctac atcatcgccg ccgtcttcaa tctccgcatc
     3001 cctgacaccg gcgcccgtta cgcccatcag cagcgtaacc ccatcaagct gattgccgat
     3061 ttttcccgct gtttcattgt gttatggaaa gacaggctgg gccagatttc gctcgccgtc
     3121 accacgctgt tctggggcgc cggcgcgaca ctgcaattca tcgtgctgca atgggccgag
     3181 aaatccctgc acatgccgct ggacagagcc gccatcctgc aaggcatcgt cgcgctcggt
     3241 gtggcggtcg gcgcggtcgg cgcggcccgc ttcgttccct tgaaaaaatc gctgacggtg
     3301 atgccgctcg gcatcatcat gggtcttatc gtcatggtga tgacaaccgt cacctcgatc
     3361 tggatcgcct atccgctact gatcgtgatc ggcgtactat ccggttattt cgtcgtgccg
     3421 atgaatgctc tgctgcaaca ccgcggccat gtactcatga gtgccggtca ctcgattgcc
     3481 gtgcagaatt tcaatgaaaa tctatcggtc ctgatcatgc tggcgttcta tgcgatgctg
     3541 gtgatgctcg atctggatgt caatatcgtg atcatcattt tcggcctgct cgtcgccagc
     3601 accatgtatc tgatcatgcg ccgtcatgct gccaaccagc gtgaccggga ttcactggca
     3661 ttgatcggcg agcataagca gcactgacgg cgtggcacat acgttctctt gcgttttcag
     3721 tgtgtggcgt cggcataagc agtcatggca agacaaagat cggcccagct cttgcgcttg
     3781 tcgccaggct gccgcaacag ataggccgga tgataagtga cgatcagcgg ccaattccgg
     3841 tagcgatgga cagtgccgcg taatttcgat accggcgtgg ccggatcaag tcccagcagc
     3901 gacagcgcgg cagtcttgcc cagcgccacc aggatcgtcg gctgtatcaa atcgatctga
     3961 cgttgcaggt agggcatgca cgatgccgtc tcctgcggcg acggtggccg gtctttgcct
     4021 ttgtcatcgg taggacggca tttgacgata ttggcaatgt aagcattgtc accgcgcttg
     4081 agacccatcg ccaccagcat gttatcaagc agcttgccgg ctgcgccgac gaacggctcg
     4141 ccctgtatat cctcattgcg gcccggacct tcgccgatga aaagccattt cgccttttcg
     4201 tcaccaacgc cgaaagcggt ttttttgcgg ccccggcaca agctgcattg ccgacaactg
     4261 gcaactgttt ccttgagcct atcccagttc atggtagcga tggcggaagg aatgcccacc
     4321 atcgctgcgc tgtcttcctg tgccgatgtc gcctgtgaca caggcgctac cgggtccggg
     4381 atcgccagtc gccctgctgc ggacattgtc tgtggcggca cagcggcggc atctgtctgc
     4441 gtcgtatcga cggccggctg ccgccggacc caaacagggc caatgccgat ttcttccaag
     4501 atcctgaccg aagcggccaa tggctgttga ctcatagcgg taaactcatc acgatcgcgt
     4561 cttcgcgcgt atgttgatcg acgggataat aatttttacg gcgtccgatt tcgacaaagc
     4621 catgacgtct gtagacatgc aacgcgcgca gattcgatac ccgcacttcc agcaacatga
     4681 tcttcatctg acgccgacgc gccagatcga cggcatggcg cagcagcaaa tgtccgaaac
     4741 cttcaccctg tacttcctca gccacgctga ggttgagcag atgcgcttcg tccatggctt
     4801 ccatcatcaa aaaataagca agcaaccggc ctgcggcgtc gcgcatggtc agtgcctggt
     4861 aaccgctgcg cagagcatcc tgaaaattgc cgcatgtcca agggcgagta taaatcctgt
     4921 cctcgatcag catgagttcg tccaggtcgg ccatctgcat ggcagcaaac gccagcttgc
     4981 cgaacctgct ttccaattcg cgcaacaggg cgtccagact caccccgcac ctttgacagc
     5041 gtcacgcacc tgccgctccg cagtggtcaa tgcgaccttg ttgcgcacat ataacggctg
     5101 cgctgcctgc ggtgaaacca tattgccctg cgcaaaagcg cggtgaccca gcgtggcgac
     5161 ctgcgccgcg tgcggcatca cctgttccag tccggcagcg gcaaaagtct gcattgctac
     5221 atcctgcgca tagacagtca ggccattacc gcacgcctgc gcggcgcctt gcggcaacac
     5281 atcatgcgca gcggacaagg tcggcgtgat caccggctgc cagccatgcg taccatggcg
     5341 atactgcgcc caatacactt ctcccatacg tgcgtcgagg atcgccaata cctcctcaga
     5401 tccgacggtt tccaggcaag cctgcgccat cgcttccagc gtgaccaccg gcaccaccgg
     5461 taaatcgcta ccgaaagcca gtccctgcgc cacgccgcaa gccgtgcgca ccccggtaaa
     5521 cgagcctggg ccgatgccga gcgcaatcgc gtcgcactgc gacaacgcta tgccggcctg
     5581 cgccagcaat tgctgcacca tcggcaaaat gcttgcggca tgcatctgta cgcccatcga
     5641 ttcccggaca atcaacgcat caccatgcag caaagctgcc gaggcgagtt cagtagaggt
     5701 ttcaatggca agaataatag acatcctgct attttaccgt gcagaattta ttttcccatc
     5761 cggagtaaaa taagcgtcct atgtcctatc agattttttc cagccgtaac cgtgaagaac
     5821 tcgccgccgc cctgcgcagc gataaatgga tcgttgcctg cctgtgcgca agctggtgtg
     5881 acagttgccg cgactatgca gtccattttg ccgcttgggc cgaacgtcac ccgcaacacc
     5941 actttgtctg gatcgatata gaagaccagg ccgatttggt tggcgatctg gacatcgaca
     6001 gcttccctac cttgctgatg cagcatggcg cgacgatcag tttcttcggc accatggaac
     6061 ccgatatccg tcaagccgaa cgcctgctgc aaaccttgtc atcgagaagc gcagccgacc
     6121 tcggccgcga agcacacagc accaaagaac accggatctg gcaggaatac gacttgcggc
     6181 agcgtctcga cgccctcccg gacgccgccg gggctgcctg agccggacgg agtctgcgat
     6241 ttcgccaaga ccattctggc gcctttttgc ttgttatttc cggccccgac atgtagagtt
     6301 gcggacttgt ttcctagcga tatttatcgc gattgcagca gtcaatcagc agcatcaaga
     6361 atactcagtc atacaatctt cccatggctt ta
//
LOCUS       DASRDH010000236         5844 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_848487,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000236 DASRDH010000000
VERSION     DASRDH010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 5844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5844
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_848487"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(166..588)
                     /locus_tag="VFA14_05470"
     CDS             complement(166..588)
                     /locus_tag="VFA14_05470"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HZS80859.1"
                     /translation="MTRIDFHSNVANRFAYACRLVRKARAARCRIVLFCKDRSELAQL
                     DQLLWTFSEQDFLPHVHAGDPLAVQTPIILTDSETVVLPHHHVLINLSGDTPEHFARF
                     ERMIEVISPDEADKTAGRNRYRSYKELGYPLSHFVADQ"
     gene            complement(700..866)
                     /locus_tag="VFA14_05475"
                     /pseudo
     CDS             complement(700..866)
                     /locus_tag="VFA14_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013957934.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase dimerization
                     domain-containing protein"
     gene            972..2012
                     /locus_tag="VFA14_05480"
     CDS             972..2012
                     /locus_tag="VFA14_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019924644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HZS80860.1"
                     /translation="MDVPDLLTFSVKNHASDLHLSTGLPPMLRINGEIRRLDLPTLAV
                     QDIDTMLDAVMDEAQANAYRRDLECDFSFEIAGLARFRVNAFHQLRGPAAVIRTIPHD
                     IPSLTQLGAPAILAELALRRQGLILVTGPTGSGKSTTLAAMVRHINEHCPAHILTVED
                     PIEFIHRSDKSLISQRAVGAHTHTFANALRAALREDPDVILVGELRDLDTIRLALSAA
                     ETGHLVLATLHTTSAAKSIDRIIDVFPGDEKDMARSMLSESLSAVVSQILLKSRRGDS
                     RIAAHEIMLGTPAIRNLIRAGKVAQMVSVMQTAVGVGMQTLDHCLAELLRKDLITPAT
                     ARAAARTLESLH"
     gene            2127..2612
                     /locus_tag="VFA14_05485"
     CDS             2127..2612
                     /locus_tag="VFA14_05485"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008331823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HZS80861.1"
                     /translation="MHSIYDFRPALPDNTPASLEPYRGKVLLIVNTASKCGFTPQYQG
                     LENIYRQFRDQGLAVLGFPCNQFGAQEPGTATEIGAFCEKNYGVSFPLFAKIDVNGDQ
                     ADPLFTYLKNQAPGLLRSEAIKWNFTKFLVRKDGSVFGRYAPAIKPEEMLADIEKLLA
                     E"
     gene            2691..3395
                     /gene="folE"
                     /locus_tag="VFA14_05490"
     CDS             2691..3395
                     /gene="folE"
                     /locus_tag="VFA14_05490"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007882400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HZS80862.1"
                     /translation="MATDQAPSNLHAEPVSARIRARIKAAGMRFHANDNIAGFILDGE
                     LEALQAEVQARLADVLESLVIDVESDHNTQDTARRVAKMYVQEVFRGRYQPPPPVTEF
                     PNIERLNELMIVGPITVRSACSHHLCPIMGKVWAGVMPNEHSHLIGLSKYARLIEWIM
                     CRPQIQEEAVSQAADLLMQKLQPDGLAIVMEADHFCMHWRGVKDIGSKMINSVMRGSF
                     LCNANLRREFLALIRK"
     gene            3431..3856
                     /locus_tag="VFA14_05495"
     CDS             3431..3856
                     /locus_tag="VFA14_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008333777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BLUF domain-containing protein"
                     /protein_id="HZS80863.1"
                     /translation="MLVRLVYASRACKPISPALIDAILTTARCHNPANGITGVLCHGD
                     DMFVQALEGGRDQVNRLYLRLLQDKRHKDVTLLCYQEIGVRQYASWTMGKAGLDKLNR
                     SLLLKYSSTGMFNPYDVSGCATVALLEELATAGAFAVRD"
     gene            complement(3903..5486)
                     /locus_tag="VFA14_05500"
     CDS             complement(3903..5486)
                     /locus_tag="VFA14_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007879396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS80864.1"
                     /translation="MASREDLTIIRAARAGQAEAQLALGRRYLLGGNGLPQSFTTALY
                     WLDRAARQDIADAWFLIGEYIPYDAVAQAPNPLAYVCWYERAFDGGLKAAGLAFARLV
                     LAYREQLAMQPMLGKALQVLEEAARVGAVEAQWLLAQQQVDLPHPAAAPSILSASAGG
                     QETADAAGWTQKAADAGVAPAQLALADAAWLRLDREDFIRHALPLGRALLARYGGELR
                     QPVLAGHLSEDNIVLLSRLSQVLSDIDGIGVDEARQLLELAAYADDKVAQCSLGLFFA
                     RMREDGSRTFPGFGLANYKKAVRWLTQAGERGLASAWFALSRIYLKPEFSQRNLTDAQ
                     HYLERAAEMGHALAQLECGSIAWRNRRDDPANDVRAVYWLQKAAAQNNAAARDLLDRI
                     VDRPARADWAVEARRHMTRESLHAYPLLAARLELAILCGLTRPEALLIDLGQADCGHC
                     LVVDIRHHHARSKRRLISIDTGAQRQALDRIMCVFEDVDCSLSGPEGNYRQRMYRLKT
                     ALSQVKDERGEKDDPAQES"
     gene            5701..>5844
                     /locus_tag="VFA14_05505"
     CDS             5701..>5844
                     /locus_tag="VFA14_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017449725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HZS80865.1"
                     /translation="MKKSLLALAVLGAFAGAAQAQSSVTIYGIVDTGVTFTNKVGTGN
                     LTGD"
BASE COUNT         1288 a         1777 c         1609 g         1170 t
ORIGIN      
        1 tgtcgacctg gaccagtagc acctgcatga gatcctcctg aatctcctgg cggatttgct
       61 gaagccaggc agccgtcagc gctggtgttt ccgctgcggc aggcatggtg atgatttcgg
      121 tgagaagcgg gatagtgttg tcggttgtct ggttcatgaa agcacttatt ggtctgcgac
      181 gaaatggctc agaggataac cgagttcctt ataggagcgg taacggttgc gaccggcagt
      241 cttgtcggct tcgtccgggg aaatgacctc gatcatgcgc tcgaagcggg cgaaatgctc
      301 cggtgtgtcg ccggacaaat tgatcagcac atgatgatgc ggcaagacga cggtttcact
      361 gtcggtcagg atgatcggcg tttgcacagc cagtgggtca ccggcatgca catgcggcaa
      421 aaaatcctgc tccgaaaagg tccacagcaa ctgatctaac tgggccaact cactacgatc
      481 cttgcagaac aacacgatcc tgcaccgcgc ggcgcgcgcc ttgcgcacca ggcgacaggc
      541 gtaagcgaat ctgttggcaa cgttgctgtg aaaatcgatg cgagtcatgc gcgcagtata
      601 tagacatatc cagcagagag gaaaacatag aatcgaaaag gatgagaaga tgctgcctgt
      661 cacctggcgt agcaaggtca gcaatgccgc gcgctgaaat cagtgtcccg gcccagctct
      721 tcccccctgc tctttccctc tcgcagcagc tgccttgacg gccatcacga tgctttgcct
      781 gatgccggta ttttccatgc tggtcagcgc ggcgtgtaaa tgatgccgcc tttggaagtc
      841 actcgctcgc gcaacagcgc ggccggatga cgtcgcattg caccagctga caacttgcaa
      901 ctgttttgcg attgacgaca taatcctatg gttcgggagg gacaacacta ttttcaggta
      961 agaaattata aatggacgtt cccgatctgc tcaccttttc ggtcaagaat catgcctcgg
     1021 atctgcatct ctccaccggt ttgcccccca tgctgcgcat caacggcgag atacgccgcc
     1081 tcgatttacc gacattggcg gtgcaggaca tcgacaccat gctcgatgcg gtcatggatg
     1141 aagcccaggc aaatgcttac cggcgcgatc tcgaatgcga cttttccttc gagatcgcag
     1201 ggctggcgcg ctttcgcgtc aatgcctttc atcaattgcg cggtccggcg gccgtcatcc
     1261 gcaccatccc gcacgacatc ccaagcctga cgcaattggg tgcgcccgcc atcctggctg
     1321 aactggcact gcggcggcag gggctgatcc tcgtgaccgg ccctaccgga tcgggaaaat
     1381 cgaccacgct ggcggccatg gtccggcata tcaacgagca ttgtccggcg catatcctga
     1441 ctgtggaaga tcccatcgaa ttcattcatc gatccgataa aagcctgatc agccagcgtg
     1501 ccgtcggcgc gcatacgcat actttcgcca atgccctgcg cgctgcctta cgtgaagatc
     1561 cggatgtgat actggtcggt gaactgcgtg acctcgacac cattcgtctc gccttgagcg
     1621 cggctgaaac cggtcatctg gtgctggcca cactgcatac cacgtcggca gccaagagca
     1681 tcgatcgcat catcgatgtt ttcccgggtg acgaaaaaga catggcacgc agcatgctgt
     1741 cggagtcgct cagcgccgtg gtgtcgcaaa tcctgctgaa aagcagacgc ggcgacagcc
     1801 ggatcgccgc ccacgaaatc atgctcggca cgcccgccat ccgcaacctg atccgcgcag
     1861 gcaaagtcgc gcaaatggta tcggtcatgc aaacggctgt cggcgtcggc atgcagacac
     1921 tcgaccattg cctggccgaa ttgctgcgca aggatctgat cacgccggca accgcgcgcg
     1981 ccgcagcgcg gacactggaa tcactgcact gaaaacgtgt ttgcaaaaca accggacgcc
     2041 cccccggcga gatcgtaaac acgttccgaa gactattccg gctatcatgc atgcttctcc
     2101 atccattcca tgctgacgag gtgaccatgc actccattta cgatttccgt ccggcactgc
     2161 ccgacaacac accagcctcg ctcgaaccct atcgcggcaa agtactgctg atcgtcaaca
     2221 ctgccagcaa atgcggtttc acaccgcaat accagggcct ggaaaacatc tatcgtcagt
     2281 tcagggacca agggcttgcc gtgctgggct ttccctgcaa tcagttcggc gcgcaggaac
     2341 cgggaacagc cacggaaatc ggtgcttttt gcgaaaaaaa ctatggtgtc agttttcccc
     2401 tgttcgccaa gatcgatgtc aacggcgatc aggccgatcc gctgtttact tatctgaaaa
     2461 accaagcgcc cggcctgctg cgcagcgaag cgatcaaatg gaatttcact aaattcctgg
     2521 tccgcaagga cggcagcgtt ttcgggcgct atgcgccggc catcaagccc gaagaaatgc
     2581 tggccgacat cgagaaattg ctggctgaat gagcctgcgt tcaacagcgc ggtttcgggg
     2641 cataatgacg gtcgtttcat tcgcattgcg tcatcgcgca ctatttcaca atggcaacag
     2701 accaagcacc atcaaatttg cacgccgagc cagtgtcagc gcgtatccgc gcgcgcatca
     2761 aggctgccgg tatgcgcttt cacgccaacg acaatattgc cggcttcatc ctggatgggg
     2821 aactggaggc tttgcaggcc gaggtgcagg ccagactggc tgacgtgctg gaaagcctgg
     2881 taatcgacgt cgagagcgat cacaatacgc aggataccgc caggcgcgtg gccaaaatgt
     2941 atgtgcagga agtctttcgc ggacgttatc agccgccacc gccggtgacc gaatttccta
     3001 acatcgaacg cctgaacgaa ctgatgatcg tcggtccgat cacagtgcgc agcgcctgct
     3061 cacatcactt gtgcccaatc atgggcaagg tctgggccgg cgtgatgccg aatgagcatt
     3121 cccacctgat cggcctgtcc aaatatgcgc gcctgatcga atggatcatg tgccgtccgc
     3181 agatccagga agaggcagtg tcgcaggcag ccgatttgtt gatgcaaaaa ttacagccag
     3241 atggtctggc catcgtgatg gaagccgatc atttctgtat gcactggcgc ggggtcaagg
     3301 acatcgggtc gaaaatgatc aatagcgtca tgcgcggatc ttttctgtgc aatgccaatt
     3361 tgcggcgcga gtttctggcg ctgatcagga aatgaattca aaaccagtcc aaccatgaac
     3421 aaggagcatc atgctggtcc gtctcgtcta tgcaagccgt gcctgtaaac cgatcagtcc
     3481 cgcgctgatc gatgcaattc tgacgacggc gcgctgtcat aatcctgcca acggcatcac
     3541 cggcgtgctg tgtcatggtg acgatatgtt cgtgcaggcg ctggagggcg ggcgcgacca
     3601 ggtcaaccgg ctttacctgc gtctgctgca agacaaacgc cacaaggacg tgacgctgct
     3661 ctgttaccag gagatcggtg tacgccagta tgcgtcctgg accatgggca aggccggact
     3721 ggacaagctc aaccgctcgc tgctgctgaa gtattccagc accggcatgt tcaatcccta
     3781 tgatgtgtcg ggttgcgcga cagtggcgtt gctggaagaa ctggcgaccg ccggcgcatt
     3841 tgccgtgcgg gactgaaagc ctctaacaca ctgctgtcaa gtgatcgacg ggaacctgca
     3901 catcaggatt cctgcgccgg gtcgtccttt tctccgcgct catcctttac ctgcgacagt
     3961 gctgttttca gccggtacat gcgctgacgg tagttgcctt ccggtccact caaactacaa
     4021 tcgacatcct cgaacacgca catgatgcga tcgagcgcct gccgttgggc gcccgtatct
     4081 atcgatatca ggcgccgctt gctgcgcgca tggtggtggc ggatgtcgac caccagacaa
     4141 tgaccgcagt cggcttgccc aaggtcgatc agcaaggctt ccggccgcgt caggccgcac
     4201 aggatcgcca gttccagccg tgctgccaga agcggatagg catgcaagga ttcacgcgtc
     4261 atgtgacgcc tggcctcaac cgcccaatcg gcgcgggcgg gacggtccac gatcctgtcc
     4321 agcagatcgc gggcggcggc attgttctgc gcagccgctt tctgcagcca gtagacagcg
     4381 cgcacatcgt tggcagggtc atcacggcga ttgcgccagg cgatactgcc acattccagt
     4441 tgcgccaggg cgtgccccat ttcggcggca cgctcgaggt aatgctgggc atcagtcaga
     4501 ttgcgctggg aaaattccgg cttcagataa atccgcgaca aggcaaacca tgccgatgcc
     4561 aacccgcgct cgccagcctg ggtcagccag cgtaccgctt tcttgtaatt ggccaggcca
     4621 aaaccgggaa aggtgcggct gccatcttcc cgcatgcgcg caaagaacaa gcccagcgag
     4681 cattgcgcca ccttgtcatc ggcataggcg gccagttcca gcaattgccg agcctcatcg
     4741 acgccgatgc cgtctatgtc ggacaacacc tgcgacaagc gcgacagcaa gacgatattg
     4801 tcttcgctca aatgcccagc cagcaccggc tgccgcaatt cgccgccgta ccgcgccagc
     4861 aacgcacgcc ccagcggcaa ggcgtgccgg atgaagtctt cccgatccag gcgcaaccac
     4921 gccgcatcag ccaacgccag ttgcgccggc gcgacgccgg catcggcagc tttttgcgtc
     4981 catccggcag catccgccgt ttcctgcccg cccgcgcttg cgctgaggat ggatggcgcc
     5041 gctgctggat gcggcaggtc cacctgctgc tgcgccagca accattgcgc ctcgacagcg
     5101 ccgacgcgcg ccgcctcctc cagcacctgg agcgccttgc ctaacatcgg ctgcatcgcg
     5161 agctgctcac gatacgccag caccagccgc gcaaacgcca gaccggcggc tttcagtccg
     5221 ccatcgaagg cgcgctcata ccagcacaca tatgccagcg gattcggcgc ttgcgctacc
     5281 gcgtcataag gaatgtactc tccgatcagg aaccaggcat ccgcgatatc ctggcgcgcg
     5341 gcgcggtcca gccaatacag cgccgtggtg aaactttgtg gcaaaccatt gccgccgagc
     5401 agatagcgcc gccccaaagc caactgagcc tcagcctgac cggcgcgtgc tgcccgaata
     5461 atcgtcaaat cttcccgact tgccattgat cacatcaatc aaaaaatttc cccggcagga
     5521 aatacatacg aagaaaatcg ttggaaaaaa acaacgtttg tacaggaatt ctggtatttc
     5581 aaccaaaaca ttgctcgcca gacagccaat cagagatcgt ggccgatgag aaccctttaa
     5641 tgtctcaggg tccggccaaa tgtacggatc ctcaagactg tttttataaa aggatgttaa
     5701 atgaaaaaat ctctgctcgc acttgccgtt ctcggcgcat ttgcaggtgc tgcacaagca
     5761 caaagctccg tcaccatcta cggtatcgtc gataccggag tcacctttac caacaaagtt
     5821 gggaccggca accttaccgg cgat
//
LOCUS       DASRDH010000237         3541 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1431022,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000237 DASRDH010000000
VERSION     DASRDH010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 3541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3541
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1431022"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..866
                     /locus_tag="VFA14_05510"
     CDS             <1..866
                     /locus_tag="VFA14_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007878114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HZS80866.1"
                     /translation="TYNHDFARLKQAFVEFTQRLPFYGVAVLCLDDPNVREIMPMISK
                     PILTYGFHEDADIHAIDARAVGGKMEFTVIQDGYAPMQVSLNQPGMHNVQNACAAIAI
                     ARELDVADADTQKALTEFNGVGRRFTRYGDVPLFDGAGRPAGWFTLIDDYGHHPVETA
                     ATIAAARSAYSGRRLVLAFQPHRYTRTRDLFEDFVKVLSMSDVLVLAEVYAAGEQPVI
                     AADGRALAHALRVAGKVEPVFVEDIADMPATILNVVRDGDVVITMGAGSISSVPGKLT
                     VQSPDRQGQGA"
     gene            878..1885
                     /locus_tag="VFA14_05515"
     CDS             878..1885
                     /locus_tag="VFA14_05515"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007878111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HZS80867.1"
                     /translation="MQFGKVGVLYGGRSAEREVSLMSGAGVLKALRSKGVDAYSFDPA
                     ERSLAELAAEKFDRVFIALHGRYGEDGTLQGALEQLGIPYTGPGVMASAMAMDKIMTK
                     RIWLGHDLPTPRFVVLDSAATLREDLCQVPDVLGLPLMLKAPHEGSTIGIVKVSGYSG
                     MQDGFDLCARYDAVVLAEEFISGRELTVPVLGQGRHGKNRARALPVVEVCAPEGNYDY
                     RHKYFTDDTRYLCPAPLDAVLTQRVQELAVQAFNALGCRGWARVDFMLRAADNEVFLL
                     EINASPGMTAHSLVPMSARQAGMSYEDLCCEILKSAALDLIPSEDWTPYLRADSNVLE
                     G"
     gene            1895..2668
                     /locus_tag="VFA14_05520"
     CDS             1895..2668
                     /locus_tag="VFA14_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008116410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HZS80868.1"
                     /translation="MWQDVKMLNAITNALLGLVLLTWLASGLWWLAQWPIFALKTIRV
                     EGMAEEPLHRVNRLTIRAGAVPRIKGSFFTADLDAARAAFESVPWVRKATVRREWPDK
                     LIVMIEEHRALGTWGDDGRLLSVKGDVFTANLAEAEDDGELLKFNGPEGSEQDVVAYY
                     RRFCDWLAPIEVMPETVVLSNRYAWTIKLDNGMTMELGRAESDAALKERIDRLVAVYP
                     QLMEGLKGKKIEHVDMRYPNGLALKVDGLLPVASSGKKK"
     gene            2679..>3541
                     /gene="ftsA"
                     /locus_tag="VFA14_05525"
     CDS             2679..>3541
                     /gene="ftsA"
                     /locus_tag="VFA14_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014898179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HZS80869.1"
                     /translation="MRKDAKNLIVGLDIGTSKVVVVVAEILPDGRHEVIGLGQSESRG
                     LKKGMVVNIEATVESIQRALEEAELMTDCKIRNVYTGIAGSHIRSFNSSGMVAVKEKE
                     VSAADVSRVIETAKAVNIPTDQQLLHTVPQEFVVDNQEDVREPIGMSGIRLEVKVHIV
                     TGAVSAVQNIVKCVRRCGLEVSDLILQPMASADAVLTPDERELGVVLVDIGSGTTDVA
                     VFTEGAIRHTAVIPIAGDQITNDIAMALRTPTLEAEEIKLRYGMAKQVLVDPAETLEV
                     PGLGDRNPRTLS"
BASE COUNT          738 a          900 c         1151 g          752 t
ORIGIN      
        1 aaacttacaa tcacgatttc gccaggctca agcaggcttt cgtcgagttc acccagcgtc
       61 tgccgttcta tggtgttgcc gtgctgtgct tggacgatcc aaatgtgcgc gaaatcatgc
      121 cgatgatttc caagcccatt ctcacttatg gcttccatga ggatgcggac attcacgcta
      181 tcgatgccag ggctgttggc ggcaagatgg aatttacggt catccaggat ggctatgcac
      241 cgatgcaggt cagtctgaat cagccaggca tgcataatgt gcaaaacgct tgtgccgcga
      301 ttgcgatcgc gcgtgagttg gatgttgctg atgccgatac gcaaaaggcg ctgaccgaat
      361 tcaatggcgt cggccgccgc ttcacgcgct acggcgacgt gccgctgttc gatggcgccg
      421 gccggccggc cggttggttc actttgatcg acgactatgg ccatcatccg gtcgagactg
      481 ctgccaccat tgctgctgcg cgcagcgctt attctggacg ccgtctggtg ctggcgttcc
      541 agccacaccg ctatacccgc acgcgcgatc tgttcgagga tttcgtcaaa gtgttgtcga
      601 tgtcggacgt actggtgctg gctgaagtat atgccgctgg cgagcagccg gttatcgccg
      661 cggacgggcg cgcactggcg catgccttgc gtgtcgcggg taaggtggaa ccggtgttcg
      721 tcgaagacat cgccgatatg ccggcaacga tactcaatgt cgtgcgtgac ggcgacgtcg
      781 tcatcaccat gggcgcgggt tcgatcagca gtgtgccagg aaaattgact gtgcagtcgc
      841 cagacaggca agggcagggg gcgtgatggc gcataccatg caattcggca aagtcggcgt
      901 actgtacggt ggtcgctccg ccgagcgcga ggtgtcattg atgtccggcg ctggcgtact
      961 caaggccttg cgcagcaagg gcgtcgatgc gtattctttc gatccggccg agcgtagcct
     1021 ggcagaattg gcggcagaaa aattcgaccg cgttttcatc gcactgcatg gccgctatgg
     1081 tgaggatggc accctgcaag gcgcgttgga gcagcttggc atcccttaca ccggccccgg
     1141 cgtgatggca tcggcgatgg cgatggacaa aatcatgacc aagcgtatct ggctcggcca
     1201 tgatttgccg acgccgcgct tcgtcgtact cgacagcgcc gctactttgc gtgaagacct
     1261 gtgccaggtg cccgacgtgc tcggcctgcc gctgatgctg aaggcgccac atgaaggttc
     1321 gaccatcggt atcgtcaagg tgagcggcta ttctggcatg caggatggct tcgatctgtg
     1381 cgccagatac gatgcggtgg ttctggcgga agaattcatc agcggtcgtg aactgactgt
     1441 gccggtgctg ggccaaggcc ggcatgggaa aaacagggcc agggccttgc cggtggtcga
     1501 ggtttgcgcg ccggaaggca attacgacta tcggcacaag tatttcaccg acgatacccg
     1561 gtatctatgc ccggcgccgc ttgacgcggt gctgacacag cgcgtgcagg aacttgcggt
     1621 acaggcgttc aatgcactcg gctgccgtgg ctgggcgcgc gtggatttca tgctgcgcgc
     1681 agcagacaac gaagtcttcc tgctcgaaat caatgcttcg cccggtatga cagcgcattc
     1741 actggtgccg atgtcggcca gacaggctgg tatgagttat gaagatttgt gctgcgagat
     1801 tctgaagtcg gcggcgctgg atttgatacc ctctgaggac tggacgccgt atcttcgggc
     1861 cgactccaat gtgttggaag gatgaaaatc gaccatgtgg caagacgtca aaatgctcaa
     1921 tgccatcacc aacgccttgc tggggctggt gctgctgaca tggttggcgt ccggtctgtg
     1981 gtggctggcg caatggccga tatttgcgct gaagacgatc cgtgtggaag gcatggccga
     2041 ggagccgctg caccgtgtga atcggctgac gatacgtgct ggtgcggtac cgcgtatcaa
     2101 gggcagtttt ttcaccgctg atctggatgc ggcgcgcgcg gcattcgagt cggtgccatg
     2161 ggtgcgcaag gcgacggtgc ggcgtgaatg gccggacaag ctcatcgtga tgatagaaga
     2221 acatcgcgcg ctcggtactt ggggcgatga cgggcgcttg ttgtcggtta agggagatgt
     2281 gtttactgcc aacctggcgg aagcggaaga cgatggtgag ctgctgaaat tcaatgggcc
     2341 ggaaggcagc gagcaagatg tcgtggcata ttaccgccgg ttttgtgact ggttggcacc
     2401 gatcgaggta atgccggaaa cggtggtttt atccaaccgc tatgcctgga ccatcaaatt
     2461 ggataatggc atgacgatgg aactgggacg tgcggaaagt gacgctgcat tgaaggaaag
     2521 gattgacagg ctggtcgctg tctatccgca gttgatggaa ggcttgaagg gtaaaaaaat
     2581 cgagcatgtt gacatgcgct atccgaacgg tttggcattg aaggtggatg ggctgctgcc
     2641 agttgcatcg agcggaaaga aaaagtgagc ggagcgatat gagaaaagat gcgaaaaatc
     2701 tgatcgtcgg tcttgacatt ggcacctcca aggtggtggt cgtagtggcg gaaatcctgc
     2761 cggatgggcg tcatgaagtg atcggcctgg gccagtccga atccaggggc ttgaagaagg
     2821 gtatggtagt caatatcgaa gctacggtcg agtcgataca gcgcgcgctg gaagaagcag
     2881 agctgatgac cgattgcaag atccgtaatg tttataccgg catcgcgggc agccatatcc
     2941 gcagcttcaa ttcgagcgga atggtggcag tcaaggagaa ggaagtcagc gctgcggatg
     3001 tctcgcgcgt catcgaaacc gcgaaagccg tcaacatccc gaccgaccag caactgctgc
     3061 ataccgtacc gcaggaattc gtcgtagata accaggaaga cgtgcgtgaa ccaatcggca
     3121 tgagcggcat ccggctcgaa gtaaaagtac atatcgtgac cggtgcggtg tcggcggtgc
     3181 aaaacattgt caagtgtgtg cgccgttgcg gtctggaagt gtcggatttg attctgcaac
     3241 cgatggcctc ggctgatgcc gtgctgacgc cggacgagcg tgagttaggt gtggtgctgg
     3301 tcgatatcgg tagcggcacg accgacgtgg cggtgtttac cgaaggcgca attcgtcaca
     3361 ccgcagtgat tccgatcgcc ggcgatcaga tcaccaacga cattgcgatg gccttgcgca
     3421 cgccaacact ggaagctgaa gaaatcaagc tgcgctatgg catggccaag caggttctgg
     3481 tggacccggc cgagacgctg gaagtccctg gattgggcga ccgtaatcca cgcaccttgt
     3541 c
//
LOCUS       DASRDH010000238         3219 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1004725,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000238 DASRDH010000000
VERSION     DASRDH010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3219
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1004725"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2405
                     /locus_tag="VFA14_05530"
     CDS             <1..2405
                     /locus_tag="VFA14_05530"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006029840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0032549 - ribonucleoside binding [Evidence
                     IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HZS80870.1"
                     /translation="TPEGPNIGLINSLALYARLNEYGFLETPYRKVENGKVTNQINYL
                     SAIEEGRYIIAQANATIDGSGKLSDELVSAREAGETILVSPERVHYMDVAPGQIVSVA
                     ASLIPFLEHDDANRALMGANMQRQAVPCLRPEKPLVGTGVERTVAVDSGTTVQALRGG
                     VVDYVDAGRVVIRVNDEEAQAGEVGVDIYNLIKYTRSNQNTNINQRPIVKVGDRVARH
                     DVIADGASTDLGELALGQNMLVAFMPWNGYNFEDSILISEKVVADDRYTSIHIEELSI
                     VARDTKLGAEEITRDISNLAENQLARLDESGITYIGAEVEAGDTLVGKVTPKGETQLT
                     PEEKLLRAIFGEKASDVKDTSLRVPSGMVGTVIDVQVFTREGIQRDKRAQQIIDDELK
                     RYRLDLNDQLRIVEGDAFQRLEKMLIGKVANGGPKKLSKGSKLTKEYLDNLDRYHWFD
                     IRPADDDMATALEAIKESIAEKRHQFDLAFEEKRKKLTQGDELPPGVQKMVKVYLAVK
                     RRLQPGDKMAGRHGNKGVVSRILPVEDMPHMADGTPADIVLNPLGVPSRMNVGQVLEV
                     HLGWAAKGLGLRIGEMLNAQVRIAELRSFLSTIYNESGKKEELDKFSDQEILELASNL
                     KKGVPFATPVFDGAHESETRRMLDLAYPDHIARQLGMTPSKNQVTMYDGRTGEAFERT
                     VTVGYMHYLKLHHLVDDKMHARSTGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYG
                     ASYVLQEMLTVKSDDVNGRTKVYENLVKGDHVIDAGIPESFNVLVKEIHSLGIDIDLE
                     RD"
     gene            2663..>3219
                     /locus_tag="VFA14_05535"
     CDS             2663..>3219
                     /locus_tag="VFA14_05535"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081256.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates. Subunit beta'
                     binds to sigma factor allowing it to bind to the -10
                     region of the promoter; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HZS80871.1"
                     /translation="MKALLDLFKQVQQNEQFDAIKIGLASPEKIRSWSYGEVKKPETI
                     NYRTFKPERDGLFCAKIFGPIKDYECLCGKYKRLKHRGVICEKCGVEVTLTKVRRERM
                     GHIELASPTAHIWFLKSLPSRLGMVLDMTLRDIERVLYFEAYVVTDPGMTPLKKCQIM
                     SEDDYAAKYEEYGDDFTAFMGAEGIR"
BASE COUNT          778 a          818 c          942 g          681 t
ORIGIN      
        1 aaacgcctga aggcccgaac atcggcctga tcaactcatt ggcgctgtac gctcgcctga
       61 acgaatacgg ttttctggaa accccgtacc gcaaagttga aaatggcaag gtcaccaacc
      121 agattaatta tctgtcggca atcgaagaag gccgctatat cattgctcag gcgaatgcga
      181 ccatcgatgg ttctggcaag ctgtctgacg aactggtctc ggcgcgtgag gctggcgaaa
      241 ctatcctggt ttcgccggag cgcgtccatt acatggacgt agctccaggc cagatcgtat
      301 cggtggcagc gtcactgatt ccgttcctcg agcacgatga tgcgaaccgc gcattgatgg
      361 gcgccaacat gcaacgtcag gcggtgcctt gcctgcgtcc ggaaaaaccg ctggtcggta
      421 ccggtgtcga gcgtaccgtt gccgtggact cggggactac cgttcaggca ttgcgtggcg
      481 gcgtggtcga ttacgtcgac gccggccgtg tggtgatccg tgtgaacgat gaagaagcac
      541 aggctggtga agtcggcgtc gatatctaca atctgatcaa atacacccgc tccaaccaga
      601 acaccaatat caaccagcgt cctatcgtca aggtgggcga ccgcgtcgcc agacatgatg
      661 tgattgctga tggcgcatcg accgatctgg gcgagctggc cttgggccag aacatgctgg
      721 tggcgttcat gccatggaat ggttacaact tcgaagactc gattctgatt tctgaaaaag
      781 tagtggctga cgatcgttat acctcgattc acatcgagga attgtcgatt gtcgcgcgtg
      841 acaccaagct cggtgcggaa gaaatcaccc gcgatatttc aaacctagcg gaaaaccagc
      901 tggcgcgtct ggatgagtcc ggcatcacct acattggcgc tgaagtcgaa gccggcgata
      961 cgctggtcgg caaggtgacg ccaaagggag aaacccagct gacaccggaa gaaaagctgc
     1021 tgcgcgcgat ctttggcgaa aaagcatccg acgtcaaaga tacatcgttg cgcgtacctt
     1081 caggtatggt cggcaccgtc atcgacgtgc aggtgtttac ccgcgaaggc atccagcgtg
     1141 ataagcgtgc gcaacagatc atcgatgatg agctaaagcg ctatcgtctg gacctgaacg
     1201 atcagttgcg tatcgtggaa ggcgatgcct tccagcgtct ggaaaaaatg ttgatcggca
     1261 aggtcgctaa cggtggcccg aagaagttgt ccaagggtag caagctgacc aaggagtatc
     1321 tggacaatct ggacagatat cactggttcg atatccgtcc ggctgatgac gacatggcga
     1381 ctgcgctgga ggcgatcaag gaatcgatcg ctgagaagcg tcaccagttc gatctggcgt
     1441 ttgaagaaaa acgcaagaaa ctgactcagg gtgacgaact gccgcctggc gtgcaaaaaa
     1501 tggtcaaggt ttatctggca gtgaagcgtc gtttgcagcc aggtgacaag atggcgggac
     1561 gtcacggtaa caagggtgtg gtgtcgcgca ttctgccggt cgaagacatg ccgcacatgg
     1621 ccgatggtac gccggctgac atcgtgctga atccgttggg cgtgccatcg cgcatgaatg
     1681 tcggtcaggt gttggaagtt catttaggtt gggcagccaa gggtctgggc ctgcgtattg
     1741 gcgaaatgct gaatgcacag gtgcgcatcg ctgaactgcg cagtttcctg agtacgatct
     1801 acaacgaatc gggcaagaag gaagagctcg ataaattctc ggatcaggaa attctggaac
     1861 tggcaagcaa cctgaaaaag ggagttccgt ttgccacgcc ggtattcgac ggtgcgcacg
     1921 aaagtgaaac ccgccgcatg ctggatctgg cctatccgga ccacatcgcc agacaactcg
     1981 gcatgacgcc gtcgaagaat caggtcacca tgtacgacgg ccgtaccggc gaagctttcg
     2041 agcgcaccgt aacggttggt tacatgcatt atctgaaact gcatcacctg gtcgatgaca
     2101 agatgcatgc acgctcgact ggtccttatt cgctggtgac ccagcagccc ttgggtggta
     2161 aggcgcagtt cggcggtcag cgcttcggtg aaatggaagt ctgggcgctg gaagcctacg
     2221 gtgcatcgta tgtgctgcag gaaatgctga ccgtgaagtc cgacgatgtg aacggtcgta
     2281 ccaaggttta tgaaaacctg gtcaagggtg atcatgtgat cgacgccggt ataccggagt
     2341 ctttcaatgt gttggtcaag gaaattcatt cgctcggtat cgacatcgat ctggaacgcg
     2401 actgatattt attaaggcaa ggcggcgtgg ctgccttgtc cggttgagcc gtctgaaaca
     2461 ggggcggcaa ggtagtggta gcaagccgtt cacgaagcag gcaaggacga ccaggtctgt
     2521 cccggtacgt gcggaacccg gcgtaagccg cggctctccc gcaggtgcga acagggtatg
     2581 agcagattgt ggtgcgaccg tttcgtcata tccgtcatac ataacgcgta catagcatct
     2641 tcacgccaac ttggagtgat acatgaaagc actgctcgat ctattcaagc aagttcagca
     2701 aaacgaacaa ttcgacgcaa tcaagattgg tttggcgtcc cctgaaaaaa tccgttcatg
     2761 gtcctacggc gaagtcaaaa agccggaaac catcaattac cgtaccttca agccagagcg
     2821 cgacggtctg ttctgcgcca agatctttgg cccgatcaag gactacgaat gtctctgcgg
     2881 caagtataag cgcctcaaac atcgcggcgt catttgcgaa aaatgtggtg tcgaagtcac
     2941 gctgaccaaa gtgcgccgtg agcgcatggg ccatatcgaa ctggcttcgc caactgcgca
     3001 tatctggttc ctgaaatcgc tgccgtcgcg tctgggcatg gtcctcgaca tgactctacg
     3061 tgatattgaa cgtgtgctgt acttcgaagc ctatgtcgtg accgatccag gcatgacccc
     3121 gctgaagaag tgccagatca tgtcggaaga cgactatgcc gccaaatacg aagagtacgg
     3181 tgacgatttc actgcattca tgggcgctga aggcattcg
//
LOCUS       DASRDH010000239         2008 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1095054,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000239 DASRDH010000000
VERSION     DASRDH010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1095054"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..641
                     /locus_tag="VFA14_05540"
     CDS             <1..641
                     /locus_tag="VFA14_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HZS80872.1"
                     /translation="ADRIEFGAATVVAGPGLGTSHHARDVLARALNTQGCIVIDADAL
                     NLIAATPDLQHRLQHRPPHTSIITPHPLEAARLLGASSAEIQHDRLQAARLLARHFKV
                     IAILKGTGTVIALPDGEIVINITGNPALSTAGTGDVLSGLCGALLAQGRPVMQAALAA
                     VWLHGKAADRLVDLGVGPVGLSASEIIPAVRALINHVMSGSDGAPAISTTLG"
     gene            791..1456
                     /locus_tag="VFA14_05545"
     CDS             791..1456
                     /locus_tag="VFA14_05545"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HZS80873.1"
                     /translation="MKALQQFIHFTEKFPGGVWGAKDTSSRCLYMNQAVRDLLRLPRD
                     LELEGLLDSEVPHPIAEFEKELQAHDRLVEVTKEMKMSLEIQPYGKDQVLSAYFFEKH
                     PFFNENNELIGTILHGYPALNLRLDRDRVNAGRIIFGVPNDILSQKEWDFVYFVMRGF
                     SVKEMARLSGVSPGTIASRQNSVFNKLGIDTLAQLKQMAVAKGWDNYLPTSLLHNRHI
                     GLW"
     gene            1599..>2008
                     /locus_tag="VFA14_05550"
     CDS             1599..>2008
                     /locus_tag="VFA14_05550"
                     /inference="COORDINATES: protein motif:HMM:NF017658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ESPR-type extended signal peptide-containing
                     protein"
                     /protein_id="HZS80874.1"
                     /translation="MNETAYRIVFNQKIGAYTTVAETASTHGKDCTIGNTASRTSSFP
                     ALTGLISLRFARWAIVIAALSGSVTTVNAQVVAYRHAPGNQQPTVLRTGNGIPLVNIQ
                     TPSAQGVSRNVYSQFDVDSKGVILNNARNSALTR"
BASE COUNT          450 a          571 c          531 g          456 t
ORIGIN      
        1 aggccgaccg aatcgaattc ggtgccgcca ccgtggttgc aggccccggc ctaggcacct
       61 cgcaccatgc ccgcgatgta ctcgcgcgtg ccctgaatac acagggatgc atcgtcatcg
      121 atgccgatgc gctgaacctc atcgctgcca ctcccgatct gcagcacagg ctgcaacacc
      181 gtcctcctca caccagcatc atcaccccgc atccgctgga agcagcccgc ctgctcggcg
      241 cctccagcgc cgagatccag catgaccggc tgcaagccgc gcgtctgctg gcgcggcatt
      301 tcaaggtcat cgctatcctc aaaggcaccg gcaccgtcat tgccctgccc gatggcgaaa
      361 tcgtgatcaa cattaccggc aacccggcgc tatcgactgc cggcaccggc gatgtcctgt
      421 ccggcctgtg cggcgccttg ctggcgcagg ggcggccggt gatgcaggcc gcactggccg
      481 cggtctggct gcatggcaag gcggctgatc gactggtcga tctcggcgtc ggcccggtcg
      541 gcctgagcgc cagcgaaatc attcccgccg tgcgtgcgct aattaaccat gtcatgtcag
      601 gatccgacgg cgcgccagcc atttccacga cgctgggatg aattgccggc tgatgtcgag
      661 tcggcgcttg cttttttcca ctgaagggcg ggtgcgcttt tcagactttt cttatatacg
      721 cataaaaacg aaggctctac tatttttaga atcatccctg atcgggcctt gatcttcaaa
      781 ggagacactc atgaaagcat tgcagcagtt tatccatttt acggagaagt tcccaggagg
      841 agtatggggg gccaaagata cgagttcccg ttgtttgtat atgaaccagg ctgtccggga
      901 tctgttgagg ttgccgcgtg accttgaact tgaaggcttg ctggatagtg aagtgcctca
      961 tccgatagct gaattcgaga aagagcttca ggcgcatgac cgcctggttg aagtcactaa
     1021 agagatgaag atgtctctgg aaattcaacc atacgggaaa gatcaggtgt tatcagccta
     1081 ttttttcgaa aaacacccat ttttcaacga gaataatgaa ttgatcggca cgattctcca
     1141 tggttacccg gcgctgaacc tgcgactcga cagggacagg gtaaatgcag ggagaattat
     1201 ttttggcgtg ccgaacgata ttttgtccca aaaagagtgg gatttcgtct attttgtgat
     1261 gcgcggtttt tcggtcaagg aaatggcgcg tttatcagga gtctcaccgg gtacgattgc
     1321 cagccggcag aattccgtct tcaacaaatt aggcatcgac acgcttgccc agctcaaaca
     1381 gatggcggtg gcgaagggtt gggacaacta tctaccgacc agtctgttgc acaataggca
     1441 tatcgggctg tggtgacggg atcttcccgc accatccgac gcccgcatgc tgtgcgaaca
     1501 ccttgaaagg gaagattttc agactattct tatatttttt gatttgataa tggcttaaac
     1561 tcaccagtcg atgattttct ggtagccggt gtggggagat gaacgagact gcatatcgaa
     1621 tcgtttttaa ccaaaaaatt ggtgcataca caacggttgc tgaaactgcc agcacccatg
     1681 gcaaagactg caccatcggc aacactgcct cacgcacttc atcattcccc gcactcactg
     1741 gtttaatctc attgcgtttc gcccgctggg caattgtcat tgctgcgctc tctggcagtg
     1801 ttaccaccgt caatgcgcaa gtagtagcct atcgccatgc tcccggcaat cagcaaccga
     1861 cggtcctgcg gactggcaac ggcatcccgt tggtcaacat ccagaccccg agcgcacaag
     1921 gtgtttcgcg caacgtctac agccagttcg acgttgacag caaaggcgtc attctcaaca
     1981 acgcccgcaa cagcgcactc acccgata
//
LOCUS       DASRDH010000240         5312 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_631403,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000240 DASRDH010000000
VERSION     DASRDH010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 5312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5312
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_631403"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            84..842
                     /locus_tag="VFA14_05555"
     CDS             84..842
                     /locus_tag="VFA14_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008111622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZS80875.1"
                     /translation="MMPRQLALVTGAARRIGRTIALALARDGWDIAVHYVRSKDEAQQ
                     VVADITALGRRAVALQCELADEAGVHRLLQQAGQFGQITCVVNNASLFCQDQADSFSM
                     ASLDAHMRTNVGAPILLAQALYDATPIGKQAVVINLLDQKLYNPNPDFLSYTLSKAAL
                     QSATTLLAQALAPRVRVVGVAPGITLASGEQSALQFDAAHRITPLGRSSTPDDVADTV
                     CFVARAPAITGTTILVDGGQHLIPLQRDVMFLAK"
     gene            878..1273
                     /locus_tag="VFA14_05560"
     CDS             878..1273
                     /locus_tag="VFA14_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008333021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HZS80876.1"
                     /translation="MFSLSHPELNDCRRLFLRNYEVLINIGVHAFEKKSEQRILINVD
                     LFIPLAESTPEKDLLQEVVDYDFIRSAIAERMAQGHIHLQETLCDDIVKAMLSHPKVC
                     AVRVSTEKPDVYPDCDSVGVEVFRVKAAP"
     gene            1295..2248
                     /gene="ttcA"
                     /locus_tag="VFA14_05565"
     CDS             1295..2248
                     /gene="ttcA"
                     /locus_tag="VFA14_05565"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HZS80877.1"
                     /translation="MHSLFGHPARAAGMPAAAPSMRQAEKTAYENNKLHKRLCRLTGQ
                     AIGDYNMIEDGDKLMVCLSGGKDSYALLDILLTLRERAPIHFDIVAVNLDQKQPNFPQ
                     EILPTYLEKLGVAYHIENQDTYSIVKRVVPEGKTTCALCSRLRRGILYRVATELGATK
                     ITLGHHRDDILETFFLNMFFSGKLKGMPPKLQSDDGRQIVIRPLAYVKEADLARYAEV
                     KQFPIIPCDLCGSQENLQRKQIKSLMREWEKQFPGRVENIFSALSTVAPSHLMDRHLF
                     DFVNLKVTGITNADGDIAFDEEPCATNQPASSQIELYRNLA"
     gene            2281..3291
                     /gene="galE"
                     /locus_tag="VFA14_05570"
     CDS             2281..3291
                     /gene="galE"
                     /locus_tag="VFA14_05570"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017449819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HZS80878.1"
                     /translation="MNILVTGGAGYIGSHTCVELLNAGHQIIVFDNFCNSFPAAIQRV
                     EKITGQSAVLVEGDIRDRAALEQVLRQHECDAVIHFAGLKAVGESIVEPLAYYANNVV
                     GTVRLLEAMQTVGVKTLVFSSSATVYGNPQYLPLTEAHPLSASNPYGRSKIMIEDMLR
                     DFYAAHPDWRIALLRYFNPVGAHESGLIGEDPQGIPNNLMPFITQVAIGKRDRLNIWG
                     GDYGTLDGTGVRDFIHVVDLAIGHLDALEYLEKAQCFTVNLGTGKGHSVLDVVKAFER
                     ACGRTLPFAIAARRPGDVAACYADVTLAKTALGWQARRSLDDMCTDSWRWQTSNPNGF
                     RT"
     gene            complement(3468..3923)
                     /gene="ybeY"
                     /locus_tag="VFA14_05575"
     CDS             complement(3468..3923)
                     /gene="ybeY"
                     /locus_tag="VFA14_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HZS80879.1"
                     /translation="MRKNKLSLSVQYADARLKAGVPRAQIRRWIQAALFAPAELTVRF
                     VDAAEGRTLNRDYRGKDYATNVLTFTYTEAGSAVTQADIILCADVLEKEAGAQKKPLT
                     DHVAHLIVHGVLHAQGYDHEEEEAAAGMEALETEILARLGFDNPYTDRG"
     gene            complement(3910..4923)
                     /locus_tag="VFA14_05580"
     CDS             complement(3910..4923)
                     /locus_tag="VFA14_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HZS80880.1"
                     /translation="MKKSSVQPTCFIPRPLDNRRLMHLCGPLDENLRQISSALDVTIF
                     RRGEKFIASGANAGRAVDILERFYSLADKPVLVEDLQLALVEQRAAMLDDTQAAADKK
                     EKTEIDSPVLKTRRHDLRGRTSHQSEYLRAILAHDITFGIGPAGTGKTYLAVACAVDA
                     LERDAVQRIVLTRPAVEAGERLGFLPGDLAQKVDPYLRPLYDALYDLLGFDRTQKLFE
                     KQAIEVAPLAYMRGRTLNHAFIILDEAQNTTPEQMKMFLTRIGFGSKAVITGDVTQID
                     LHQGQKSGLIDAIDVLCDVHGIVFSRFTSSDVVRHPLVARIVDAYAGRDLTQPAQPRH
                     AKK"
     gene            complement(4947..>5312)
                     /locus_tag="VFA14_05585"
     CDS             complement(4947..>5312)
                     /locus_tag="VFA14_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HZS80881.1"
                     /translation="TDIGYDTSFSFIFSPRPGTPAASLADDTPSEVKLKRLRHLQAAV
                     QDNADRISRDMVGTVQRVLIEGPSRKDRSELQGRTENNRVVNFDGGHDGVRLIGQMVD
                     VSIVQALPFSLRGELILEQ"
BASE COUNT         1150 a         1594 c         1471 g         1097 t
ORIGIN      
        1 attgcaatcg catgatggcg cgtattgtat tggaatcccc cctggccaga tgacatgcag
       61 catcccatga tgaaaaaaat gccatgatgc cacgacagct tgccctcgtt accggcgccg
      121 ccagacgcat cggtcgcacg attgcactcg cgctggcgcg cgacggctgg gacatcgcgg
      181 tgcattacgt ccgctcgaaa gacgaagcgc aacaggtcgt ggccgatatc accgccctcg
      241 gtcgccgcgc cgtcgcgctg caatgtgaac tggccgatga agccggcgta cacagactac
      301 tgcagcaggc cggacagttc ggacagatca cctgcgtagt caacaacgcc tcactgttct
      361 gtcaggatca ggctgatagc ttttcgatgg cgtcgctgga cgcccacatg cgtaccaatg
      421 tcggcgcacc gatcctgctg gcccaggccc tgtacgacgc cacgcccatc ggcaagcagg
      481 cagtggtgat caacctgctg gaccagaagc tatacaatcc gaatcccgat ttcctgtcct
      541 acaccctgtc caaggccgcg ctgcaaagcg ccaccaccct gctggcgcag gcgctggcgc
      601 cacgtgtgcg cgtggtcggc gtagcacccg gcatcaccct ggcatcaggc gaacaaagcg
      661 cgctgcaatt cgatgcagcc catcgcatca ccccgctggg caggagcagc acgccggatg
      721 atgttgccga cacagtctgc ttcgtggcaa gagcgccagc gatcaccggc acgacgatac
      781 tggtcgacgg cggtcaacac ctgattccac tgcaacgcga tgtcatgttt ctcgcgaagt
      841 aagaatgtgt ttctttttta cccttttacc ggacgccatg ttctctcttt cccatcctga
      901 attaaacgat tgccgccgtc tgtttctgcg caactatgaa gtgctgatca atatcggcgt
      961 gcatgccttt gaaaagaaga gtgaacaacg catcctgatc aacgtcgatc tgttcattcc
     1021 attggccgaa tcgacgccgg aaaaagacct gctgcaagaa gtagtcgatt acgacttcat
     1081 ccgcagcgcc atcgccgaac gcatggcgca aggacacatt cacttgcagg aaaccctgtg
     1141 cgacgacatc gtcaaggcca tgctgtcaca tccgaaagtg tgcgccgtgc gcgtttccac
     1201 agaaaagccc gatgtctacc cggattgcga ttcggtcggc gtggaagtgt tccgcgtcaa
     1261 agcagcaccc tgatgatttt ttgtgagaac cagaatgcac agtctgttcg gacaccccgc
     1321 acgcgccgcc ggcatgccag cagccgcgcc gtcgatgagg caggcggaaa aaaccgccta
     1381 cgaaaacaac aagctgcaca agcgtctatg ccgtctgacg ggccaagcca tcggcgacta
     1441 caacatgatc gaagacggcg acaagctcat ggtctgcctg tccggcggca aggacagcta
     1501 cgccctattg gacatcctgc tgacgctgcg cgaacgggca ccgattcatt tcgacatcgt
     1561 ggcggtcaat cttgaccaga agcaaccaaa tttcccacag gaaatattgc cgacctatct
     1621 ggaaaaactg ggtgtagcgt atcacatcga aaatcaggat acctacagca tcgtcaaacg
     1681 cgtcgtgccg gaaggcaaga ccacctgcgc gctctgttcg cgcctgcgac gcggtattct
     1741 ttaccgcgtc gcaacagaac tcggcgcgac aaaaattaca ctgggacatc atcgcgacga
     1801 cattctcgaa acttttttcc tcaacatgtt cttcagcggc aagctcaagg gcatgccgcc
     1861 gaaactgcaa tccgacgatg gcagacagat cgtcatccgt ccgctggctt acgtgaaaga
     1921 agccgatctc gcgcgttatg ccgaggtcaa gcagttcccc atcattccat gcgacctgtg
     1981 cggcagccag gaaaacctgc aacgcaagca gatcaagagc ttgatgcgcg aatgggaaaa
     2041 gcaattccca ggccgcgtgg agaatatttt cagcgcgttg tcgactgtcg cgccgtcgca
     2101 tctgatggac cgccatctgt tcgacttcgt caatctgaaa gtgaccggca tcaccaatgc
     2161 ggatggcgac atcgccttcg atgaagagcc ttgcgccacg aaccagcctg ccagcagcca
     2221 gattgagctt taccggaatc tggcataatc atccgctccg tcacgccatc tcgcatcgat
     2281 atgaatattc tggttacagg cggtgcgggc tatatcggct cccatacttg cgtagagctg
     2341 ctgaacgctg gccaccaaat catcgtcttc gacaatttct gcaacagttt tcccgcagcg
     2401 atacagcgcg tcgaaaaaat taccggtcaa tcagccgtgc tggtggaagg cgacatccgc
     2461 gatcgtgcag cgctggagca ggtcttgcgc cagcacgaat gcgacgccgt gattcacttt
     2521 gccggactca aggccgtcgg tgaatccatc gtcgaaccgc ttgcctatta cgctaacaat
     2581 gtcgtcggta ccgtgcgcct gctggaagct atgcagaccg tcggcgtcaa gacactggtg
     2641 ttcagttcct cggcaaccgt ctacggcaat ccgcaatacc tgccgctgac cgaggctcat
     2701 ccgctctcag ccagcaatcc ctatggccgc agcaagatca tgatcgaaga catgctgcgc
     2761 gatttttatg cggcgcatcc ggactggcgc atcgctttgc tgcgttactt caacccggtc
     2821 ggcgcgcatg agagcggcct gatcggcgaa gatccgcaag gcatccccaa taatctgatg
     2881 cctttcatta cgcaagtcgc catcggcaag cgcgacagac tcaacatctg gggcggcgac
     2941 tacggcacac tggacggcac cggcgtgcgc gatttcattc atgtcgtcga tctggcgatc
     3001 ggccatctcg atgcattgga atatctcgaa aaagcccagt gttttaccgt caacctcggt
     3061 accggcaaag gtcatagcgt gcttgacgta gtcaaggctt tcgagcgcgc ctgcggcaga
     3121 acgctaccct tcgcgattgc agcgcgccgc cctggcgacg tcgcggcctg ttacgccgat
     3181 gtcacgctgg caaaaacggc gctgggatgg caagcaaggc gttcactcga cgatatgtgt
     3241 accgacagtt ggcgctggca aaccagtaat cccaacggtt ttcgcacatg aatatttagc
     3301 tttgcgggga tacattctta gagccgctaa cccaaggccg atgggccgat ggcaaggcgc
     3361 actgacgcag acagtacgaa agtacggcaa ggaggcgcaa cgcagccagc agccttgtgt
     3421 tagcggctct tagcgcaggc ccatgcagtt ggatgcgctc gttcctctca gcctcgatcc
     3481 gtatacggat tgtcgaaacc cagtcgcgcc agaatctccg tttccagggc ttccatcccg
     3541 gctgctgctt cctcttcctc atggtcatag ccctgggcgt gcagcacacc atgcacgatc
     3601 agatgtgcca catgatcggt cagcggtttc ttttgcgcgc cggcttcttt ttccagcaca
     3661 tcggcacaca gaatgatgtc ggcctgggtc actgcactgc ctgcttcggt ataagtgaaa
     3721 gtcagtacgt tggtggcgta atctttgccg cgatagtcgc ggttcagcgt ccggccttcc
     3781 gcagcgtcga cgaaacggac cgtcagttcc gccggtgcaa acagtgccgc ctggatccag
     3841 cggcggatct gcgcgcgcgg cacgcctgct ttcaggcgcg catcggcata ctggaccgag
     3901 agagacaatt tattttttcg catggcgcgg ttgagctggc tgcgtcagat cccggcctgc
     3961 ataggcatcg acgatacgcg ccaccagcgg atgacgcacc acgtcgctgc tggtgaaacg
     4021 cgagaagacg atgccatgta cgtcgcacag aacatcgatc gcatcgatca ggccgctttt
     4081 ctggccctga tgcaaatcga tctgcgtgac gtcgccggta atcacggcct tgctgccgaa
     4141 gccaatacgc gtgaggaaca ttttcatctg ttccggcgtc gtgttttgcg cttcatcgag
     4201 aatgatgaag gcgtgattga gcgtgcggcc gcgcatatac gccagcgggg cgacttcgat
     4261 cgcctgtttt tcgaacagct tctgagtgcg gtcgaaacca agcagatcgt acaacgcgtc
     4321 atacaacgga cgcagataag gatcgacctt ttgcgctaga tcaccgggca gaaagcccag
     4381 acgctcacct gcttccaccg ccgggcgcgt cagcacgata cgctgcacgg catcgcgttc
     4441 cagcgcatcg accgcgcagg cgacggcaag ataagttttg ccggtgccgg ccggaccgat
     4501 gccgaaggtg atgtcatgcg ccagaatggc gcgcaaatat tcgctctgat gcgacgtgcg
     4561 gccgcgcaga tcgtgtctgc gcgtcttcag taccgggctg tcgatttcgg ttttttcctt
     4621 tttgtcagca gcagcctgtg tatcgtccag catggcggca cgctgttcga ccagcgccag
     4681 ctgtaggtct tctaccaata ccggcttgtc cgccagtgaa taaaagcgct cgaggatgtc
     4741 cacggcgcgc ccggcattgg caccgctggc gatgaatttt tcgccgcggc ggaaaatcgt
     4801 cacatccagg gcggatgaaa tttgccgcag gttctcatcc agcgggccac acagatgcat
     4861 caagcgcctg ttatcaagcg gtcgcgggat gaaacaggtc ggctggacag atgatttttt
     4921 caagctggct cgtttatagt gaggaatcat tgctcgagta tgagctcgcc gcgcagcgag
     4981 aacggcaagg cctgcacaat cgatacgtcg accatctggc caatcagacg cacgccgtcg
     5041 tgaccgccgt cgaaattgac gacgcggttg ttttccgtgc ggccttgcag ttcgctgcgg
     5101 tcttttctgg acggaccttc gatcagaacg cgctggaccg tgccaaccat gtctcggctg
     5161 atcctgtcgg cattgtcctg cacggctgcc tgcaaatgcc gcaagcgttt gagcttgact
     5221 tcggaaggcg tgtcatcggc caagctggca gccggagttc caggacgcgg actgaaaatg
     5281 aagctgaagc tggtgtcata gccgatgtcg gt
//
LOCUS       DASRDH010000241         7699 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1161089,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000241 DASRDH010000000
VERSION     DASRDH010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 7699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7699
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1161089"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="VFA14_05590"
     CDS             <1..342
                     /locus_tag="VFA14_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007879586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HZS80882.1"
                     /translation="AVVFNTIRLQIMTQHEEIDISRLLGATNAFIHRPFYYTGALLGL
                     CAGSLALIWVMLALQPLNTAIAGFARLYASEFRLVPASLSVIGFLLGLSALLGLAGAM
                     LSVRRHLSRLA"
     gene            566..1456
                     /gene="rpoH"
                     /locus_tag="VFA14_05595"
     CDS             566..1456
                     /gene="rpoH"
                     /locus_tag="VFA14_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013236077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HZS80883.1"
                     /translation="MKPATALIPVETNALALGFSGSLGNIDAYISAVNRLPMLTQAEE
                     VSLARNLRDKNDLAAAQKLVLSHLRLVVSIARSYLGYGLPHADLIQEGNIGLMKAVKR
                     FDPEQNVRLVSYAMHWIKAEMHEYILKNWRLVKVATTKAQRKLFFNLRSHKESLDAMT
                     PAQVDALAETLDVKPEEVIEMETRLSGRDVALEAPTDDEDDRFAPIAYLSSDNSEPIR
                     MMEARQYDRLQSEGLESALGKLDDRSRRIVEARWLASDDGSGATLHELAGEFGVSAER
                     IRQIEVAALKKMKGALQAFV"
     gene            complement(1529..2335)
                     /gene="otnI"
                     /locus_tag="VFA14_05600"
     CDS             complement(1529..2335)
                     /gene="otnI"
                     /locus_tag="VFA14_05600"
                     /EC_number="5.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007879500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HZS80884.1"
                     /translation="MLRFAANLSMLYTEHAFLDRFAAAARDGFSGVEFLFPYDFQPAE
                     IKSRLDAHHLTQALFNAPPGDWAAGERGIASLPGREDEFKRGIETALQYASIIGNRTL
                     HVMAGLIRPEQDQAQHRAVYISNLSYAAHQAASAGITVVIEPINTRNIPGFFLNRQSD
                     AQAICAEIGAANLKVQFDCYHCQIVEGDVAVKLKRDMAGIGHIQIAGAPERHEPDTGE
                     LNYPYLLELIDTLGYQGWIGCEYLPASSTSDGLAWLEALAAQGLTELLPR"
     gene            complement(2346..3659)
                     /locus_tag="VFA14_05605"
     CDS             complement(2346..3659)
                     /locus_tag="VFA14_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007879499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZS80885.1"
                     /translation="MIASTTSGAVANAKTQERNRVYAKVFWRIMPFLMLCYVIAYLDR
                     VNIGFAKLQMSIDLGFSETVFGLGAGVFFIGYFLFEVPSNILMHKVGARVWIARIMIT
                     WGILSAAFMYVQTPMQFYVLRFLLGLAEAGFYPGIILYLTYWYPSHRRARVIAVFMSG
                     IPVAGILGNPLSGWIMDTFHGTTGMQGWQWMFVIEAIPAVLIGLATLAYLDNGIAKAK
                     WLTGAEKKLLEEEIAADQKGKESAYSFADIIGDRRVWLVCLIYFCFVMGQYGLTLWMP
                     TLVKATGVNGNLHIGLLSAIPFGCAIIAMNLIGRSADARRERRWHLIVPAMMGATGFV
                     GAALFADSTVISVVALSLAAAGVLTCAPLFWSLPTAFLSGAAAAVGIAAINSVGNLAG
                     FVSPYLIGYLQDLTQDSAAGMYMLAIMLVIGAAAVLKTSPALVNK"
     gene            complement(3743..4381)
                     /locus_tag="VFA14_05610"
     CDS             complement(3743..4381)
                     /locus_tag="VFA14_05610"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008112643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HZS80886.1"
                     /translation="MSKEIQLREEICRTGASLYQRGYTVGSAGNISARLDDGWLITPT
                     DACLGFLDPEKIAKVDATGNWISGDQPSKTLALHRRVYENNPEVHAVVHTHSTYLVNL
                     TITGVWSQDDVLPPITPYYVMKVGHIPLISYHRPGDPQVAERVEQLATKVRGVLLERL
                     GPVVWETSVSRAAYALEELEEAARLWHLAGGNVTPLDDAALQELREVFSARW"
     gene            complement(4378..5664)
                     /gene="otnK"
                     /locus_tag="VFA14_05615"
     CDS             complement(4378..5664)
                     /gene="otnK"
                     /locus_tag="VFA14_05615"
                     /EC_number="2.7.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008332716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HZS80887.1"
                     /translation="MPQATPLLGCIADDFTGATDLANMLVRGGMRTVQTIGIPEQMPA
                     LAADALVIALKSRTIPVAEAIAQSLAALRWLQQQGCTQFFFKYCSTFDSTDAGNIGQV
                     TDALLKELGADFTIACPAFPENGRTIYRGYLFVADALLNESGMEHHPLTPMADANLVR
                     VLQRQTPSRVGLVRYDTVAKNAAAVVEHFTALRAEGVTMAIADAVSDRDLHVLGEACA
                     GLKLITGGSGIALGLPENFRRAGLLRGASEAAQLPKVDGLSAVLAGSASKATNAQVAE
                     WKSKHPAYRIDPLALVRGETVAAQALAFADAYIEQEPVLIYATATPDEVRAVQKKLGV
                     AKASHLVEQALGEIARGLHERGVRRFVIAGGETSGAVVQALGVRTLRIGPQIDPGVPA
                     TVALDGQPLALTLKSGNFGSVDFFEKALRFLGGTNV"
     gene            complement(5683..6576)
                     /gene="ltnD"
                     /locus_tag="VFA14_05620"
     CDS             complement(5683..6576)
                     /gene="ltnD"
                     /locus_tag="VFA14_05620"
                     /EC_number="1.1.1.411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007879495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonate dehydrogenase"
                     /protein_id="HZS80888.1"
                     /translation="MSNNVGVIGLGVMGLGVARSLLRAGFKVHACDVRRDVLDTFAAE
                     GGVACANPAEMGRTADVVITLVVNAAQTETVLLGEHGAASTLKRGSVVISSATVAPDF
                     AIALGQHLAEKGLLMLDAPVSGGAARAASGEMTMMTSGPANAYAKVEDVLRGMAGKVY
                     RLGDAHGIGSRVKIINQLLAGVHIAASAEAMALGLREGVNPDALYDVITHSAGNSWMF
                     ENRVPHILNGDYTPLSAVDIFVKDLGLVLDTARRSKFPLPLAAAAHQMFMMASTAGHG
                     SEDDSAVIKIFPGIDLPPSKQ"
     gene            complement(6646..7341)
                     /locus_tag="VFA14_05625"
     CDS             complement(6646..7341)
                     /locus_tag="VFA14_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008332719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HZS80889.1"
                     /translation="MFQKIPVQALSDTVARHLLEKIDRGAFPRGGKLPTEAVLTREFG
                     VSRTVVREAISRLKQEGVVEPRQGSGIFVTGQAGIKPLRIDYTALNSPAAVLQIIELR
                     RAIEAEVAAQAAKRRTNADMMAIDIALARIGQDVSNGSNGVAADVAFHRTLAMATRNP
                     YFIKTLEFLSQYLEAATTVTRGNEARRDDFSRQVHEEHEAIVAAVRTRDDMAARAAAQ
                     THMFNAARRLGQA"
     gene            complement(7578..>7699)
                     /locus_tag="VFA14_05630"
     CDS             complement(7578..>7699)
                     /locus_tag="VFA14_05630"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HZS80890.1"
                     /translation="ARPGTTAPNRFYMYGVVARLALLAASLEMEKTDPNPEIY"
BASE COUNT         1649 a         2291 c         2176 g         1583 t
ORIGIN      
        1 gcagtcgtgt tcaacaccat ccgcctgcaa atcatgacgc agcatgaaga aatcgacata
       61 tcgcgtctgc tgggcgctac caatgccttc atccatcgcc cgttctatta caccggcgca
      121 ttgctcggcc tgtgcgccgg cagcctggcc ctgatatggg tgatgctggc tttgcagccg
      181 ctgaacacgg caatcgccgg tttcgcccgc ctgtacgcct cggaattccg cctggtgcca
      241 gccagcttgt ccgttattgg cttcctgctc ggcctgagcg cgctgctggg actggccggc
      301 gccatgctat cggtcaggcg ccatctctca cgactagcct gacgctgatc acgccagccc
      361 gctttctggt gagaataaat ctggcgaatt ttctatttta gatagaatta tttattcata
      421 tatgtggaat cattcctttc ccccagagcc gtaattagca ctccctacgc aggagtgcta
      481 aaatactcga cagaaatact ccagttttaa atgcattgcc tgattgtaca catcgcttct
      541 ccactcaaaa aacaaggatg aatcaatgaa accagctact gcactgatac ccgtcgaaac
      601 caatgcgctt gcgctcggtt tctccggcag tctgggcaat attgacgcct atatttcggc
      661 ggtcaaccgt ctgcctatgc tgacgcaagc tgaagaagta tcgctggcgc gtaatctgcg
      721 cgataaaaat gatcttgcag ccgcacaaaa actggtgttg tcgcacttgc ggctggtggt
      781 ctcgatcgcc cgcagctatc taggctatgg cttgccgcac gctgatctga tccaggaagg
      841 caacatcggc ctgatgaaag cggtcaagcg cttcgacccg gaacagaacg tgcgtctggt
      901 gtcgtatgcg atgcactgga tcaaggccga gatgcatgag tacatcttga agaactggcg
      961 tcttgtcaaa gtggcaacta ccaaggcgca gcgcaagttg ttcttcaacc tgcgcagtca
     1021 caaggaaagc ctggatgcca tgacgccggc gcaagtcgat gcgctggccg aaacgctgga
     1081 cgtcaagcct gaagaagtca tagagatgga aactcgcctg tccggccgtg acgtcgcgct
     1141 ggaagcgcca accgatgatg aagacgacag attcgcgccg attgcctacc tgtcctcgga
     1201 caacagcgag ccgatccgta tgatggaagc ccggcaatat gaccgtctgc aatcggaagg
     1261 tctggagagc gcactgggca agctcgacga tcgttcgcgc cgcatcgtgg aagcgcgctg
     1321 gctggccagt gacgacggca gcggcgctac tttgcacgaa ctggccggcg agttcggcgt
     1381 gtctgccgag cgtatccgcc agatcgaggt agccgccttg aagaaaatga agggggcgct
     1441 gcaggcgttt gtctaatcag gcaaacggtc tattctcaaa ccttgctgct gttgccacgc
     1501 tgaatcagga tcagtctatg atcctgattc agcgtggcaa cagctcggtc aggccctgcg
     1561 ctgccagcgc ctcgagccaa gccaggccgt ccgacgtgga cgatgctggc aagtactcgc
     1621 agccaatcca tccctgatag cctaacgtat cgatcaattc gagcagatac ggataattca
     1681 gttcgccggt gtccggttca tggcgttcgg gtgcgccggc gatctgaatg tggccgatgc
     1741 cggccatgtc acgcttcaac ttgacggcga cgtcgccttc gacgatctgg cagtgatagc
     1801 agtcgaattg tacttttagg ttagccgcgc cgatttcggc gcagatggct tgcgcgtcgc
     1861 tctggcggtt gaggaagaag cccgggatgt tacgtgtgtt gatcggctcg atcaccacgg
     1921 tgatacctgc cgatgcagcc tgatgggcgg cgtaggacag gttgcttatg tagactgcgc
     1981 gatgctgggc ctggtcctgt tctggacgga tcaaaccggc catgacgtgc agcgtcctgt
     2041 tgccgatgat gctggcgtat tgcaatgccg tttcgatgcc gcgtttgaat tcatcttcgc
     2101 ggcctggcaa cgaagcgata ccgcgctcac ccgcagccca gtcgcccggc ggcgcattga
     2161 acagagcctg cgtgaggtgg tgcgcatcga gacgtgactt gatttctgcc ggctggaaat
     2221 catacggaaa caaaaattcg acgccgctga aaccatctct ggcggcagca gcaaagcgat
     2281 caagaaaagc atgttcggtg tatagcatgc tcaggttggc ggcaaaacgc agcatcgtga
     2341 tccctttatt tattcaccag cgcaggcgaa gtcttcaaca cagcggcagc gccgatcacc
     2401 agcatgatcg ctagcatgta catgccggct gcgctgtcct gtgtcagatc ctgcagatag
     2461 ccgatcagat aagggctgac aaaaccggct agattaccta ccgaattgat tgccgcgata
     2521 ccgactgctg cggcagcgcc cgacaggaat gccgtcggca gcgaccagaa tagcggcgcg
     2581 catgtcagca cgccagcggc ggccagcgac agagccacaa ccgagatcac tgtgctgtcg
     2641 gcaaacagcg ctgcgccgac gaaaccggtc gcgcccatca tggccggtac gatcagatgc
     2701 cagcgacgtt cgcgacgggc gtcggcgctg cggccgatca ggttcatcgc gatgatcgcg
     2761 caaccgaaag gaatcgcgct caacagaccg atatgcaggt taccgttgac gccagtcgct
     2821 ttgaccagcg tcggcatcca cagcgtcaga ccgtattgac ccatgacgaa gcagaaatag
     2881 atcaaacaca ccagccagac acgtctatcg ccgatgatat cagcaaaaga atacgcgctt
     2941 tccttgcctt tctggtcggc ggcgatttcc tcttccagca atttcttttc ggcgccggtc
     3001 agccactttg ccttggcgat gccattgtcc agatatgcca gcgttgccag gccgatcagg
     3061 actgcaggaa ttgcttcgat cacgaacatc cattgccagc cttgcatgcc agtggtgccg
     3121 tggaaggtat ccatgatcca gcctgacagc ggattgccaa gaatgccggc gacgggaata
     3181 ccggacatga acaccgcgat caccctggcg cggcgatgcg atggatacca gtaggtcagg
     3241 tacaggatga tgccgggata gaaaccggct tcggccagac ccagtaggaa gcgcagcaca
     3301 tagaactgca tcggtgtctg gacatacatg aacgccgccg acagaatgcc ccaggtgatc
     3361 atgatgcggg caatccagac gcgcgcgccg accttgtgca tcaggatatt gctcggtacc
     3421 tcgaacagaa aatagccgat gaagaatacg ccggcaccga ggccaaacac ggtttcgctg
     3481 aaaccgagat caatcgacat ttgcagcttg gcgaagccga tgttgacgcg atccaggtag
     3541 gcaatcacat aacacagcat gaggaaaggc atgatacgcc agaatacctt ggcatagacg
     3601 cggttccgtt cctgtgtctt ggcgttcgct actgccccgg atgtggttga tgcaatcatg
     3661 ctgcgacaat ctcctgttga tgcttggccg tttcgatggc cgttgatgat gcctgcgaaa
     3721 caggcaatca tggttgaatg cgctaccagc gcgcgctgaa tacctcacgt aattcctgca
     3781 aggcagcgtc atccagaggc gtcacattgc cgccggcaag atgccataag cgcgcggctt
     3841 cttctagttc ttccagcgca taggccgccc tggaaaccga ggtttcccac accaccggac
     3901 caagacgctc gagcagcacg ccacgcactt tggtggcaag ctgctccaca cgttcggcta
     3961 cttgcggatc gccggggcga tggtaggaaa tcagcgggat atggccgacc ttcattacgt
     4021 aatacggtgt gatcggtggc agtacatcgt cctgcgacca cacgccggtg attgtcagat
     4081 tgaccagata ggtcgagtgc gtatgcacga cggcatgtac ctcaggattg ttctcgtaga
     4141 ccctgcgatg cagggccagc gtcttggaag gctggtcgcc cgagatccag ttgccggttg
     4201 catcgacctt ggcgattttt tccggatcga gaaaacccag gcaggcatcg gtcggtgtga
     4261 tcagccagcc gtcatcaaga cgcgcactga tgttgccagc tgatccaacg gtatagccgc
     4321 gttgatagag actggcgccg gtgcggcaga tttcttcgcg cagttggatt tccttgctca
     4381 tacgttcgtt ccaccgagga agcgcaaggc tttctcaaaa aaatcgacgc tgccaaaatt
     4441 gcctgacttc aatgtcagcg ccaggggctg cccgtcgagc gcaacagtcg ccggaacgcc
     4501 tggatcgatt tgcggcccga tgcgtaaggt gcgcacaccg agcgcttgca ccaccgcgcc
     4561 ggacgtttcg ccgccggcaa tcacgaaccg gcgcacgccg cgttcgtgca aacccctggc
     4621 gatctcgccc aatgcctgtt cgaccagatg actggccttg gcgacgccca gttttttttg
     4681 cactgcccgt acttcatccg gtgttgcagt ggcataaatt agcactggct cttgttcgat
     4741 gtacgcatcg gcaaacgcca gtgcctgcgc tgcgacagtt tcgccacgta ccagcgccag
     4801 tgggtcgata cgataagcag gatgtttcga tttccattca gcgacctgcg cattggtggc
     4861 tttggaagca ctgccggcca gcactgccga caagccatcg accttcggca gctgcgccgc
     4921 ttcgctggca ccacgcagca aaccggcgcg ccggaaattt tccggcagac cgagcgcgat
     4981 accggagccg ccggtaatca gtttcagtcc tgcgcaggct tcgcccaaca catgcaggtc
     5041 acggtcggag accgcatcgg caatcgccat cgtcacacct tcagcacgca aggcggtgaa
     5101 gtgttcgact acagccgccg cattttttgc cactgtgtcg taacgcacca ggccgaccct
     5161 ggatggtgtc tgccgctgca acacacgcac cagattggca tcggccatcg gtgtcagcgg
     5221 atgatgctcc ataccggatt cgttgagcag cgcatcggcg acgaacagat agccgcgata
     5281 aatagtgcgg ccgttttccg ggaaagcagg gcaggcaatg gtgaagtcgg cgcccagttc
     5341 tttcagcagc gcgtcggtca cctggccgat gttgccggca tcggtcgaat cgaaagtaga
     5401 acaatacttg aagaaaaatt gtgtgcagcc ttgctgctgt aaccagcgca aggccgccag
     5461 tgattgtgcg atcgcttcgg cgaccgggat ggtgcgcgat ttgagtgcga tcaccagagc
     5521 atcggcggca agcgccggca tctgttcagg aataccgata gtctgcacgg tgcgcatgcc
     5581 gccgcgcacc agcatattgg cgaggtcggt ggccccggtg aaatcgtcgg cgatacagcc
     5641 gagcaaaggt gtagcttgag gcatgtcggg atcccgtagc gcttactgtt tcgatggtgg
     5701 cagatcgatg ccggggaaaa tcttgatcac ggcggaatca tcttcgctgc catgaccggc
     5761 agtcgatgcc atcataaaca tctgatgcgc ggcggcggcc aatggcagcg ggaatttgct
     5821 gcggcgcgcg gtatcgagta ccagacctag atccttgacg aaaatatcga ccgctgacag
     5881 cggcgtgtaa tcgccgttga gaatatgcgg cacgcggttt tcgaacatcc acgaattacc
     5941 cgcgctatgt gtgatcacgt catacagtgc atcgggattg acgccttcac gcagccccag
     6001 cgccatggct tccgccgacg cggcaatgtg gacgcccgcc agcaactggt tgatgatctt
     6061 gaccctggaa ccgatgccgt gcgcatcgcc cagtcggtag accttgccgg ccatgcccct
     6121 gagcacatct tcgaccttgg cgtaggcatt ggcaggaccg gacgtcatca tcgtcatctc
     6181 acccgatgcg gcgcgtgcgg cgccacccga caccggcgca tccagcatga gtaaaccttt
     6241 ttcagcgagg tgctgaccaa gtgcgatcgc aaaatccggc gccaccgtcg cgctggaaat
     6301 caccacgcta ccccgtttca gcgtcgaggc tgcgccatgc tcgcccaaca ggaccgtctc
     6361 ggtctgcgcc gcgttgacca ctagcgtgat caccacatcg gctgtcctgc ccatctcggc
     6421 agggttcgcg caggcgacgc cgccttcggc agcgaatgta tcgagcacat ccctgcgcac
     6481 gtcgcaggca tgcaccttga accccgcgcg cagcaacgaa cgggcaacgc ccagacccat
     6541 cacccccaaa ccgattacac ctacattgtt agacatcgtt gactccgata acagcggcag
     6601 tgtcggaccc gaaacggctt ccggcacgct gcgcgcctgc taaaattaag cctgacccaa
     6661 tcgtcttgcc gcgttaaaca tatgcgtctg cgccgcagcg cgcgctgcca tgtcatccct
     6721 ggtgcgaact gcggcgacga tggcctcatg ctcttcatgc acctgacgcg agaaatcgtc
     6781 gcgccgcgcc tcattaccgc gtgtgacagt agtggcagcc tccagatatt ggctcagaaa
     6841 ttccagcgtc ttgatgaaat acggattgcg cgtagccatc gccagggtac ggtgaaaagc
     6901 cacgtcggcg gcaacgccat tgctgccgtt actgacatcc tgaccgatgc gcgccagcgc
     6961 gatgtcgatg gccatcatgt cggcattggt acggcgcttg gctgcctgcg ccgcgacttc
     7021 cgcttcgatg gcgcggcgca gctcgatgat ctgcaggacg gctgcgggag aattgagcgc
     7081 ggtgtaatcg atgcgcagtg gtttgatgcc ggcctgtccg gtaacgaata tgccgctgcc
     7141 ctgacgcggt tcgactacgc cttcctgctt gagtcgcgag attgcttcgc gcacgactgt
     7201 acgactgacg ccgaactccc gcgtcaacac ggcctcggtc ggcaatttgc cgccgcgcgg
     7261 aaatgctccc cggtcgattt tctccagcaa gtgccgcgct accgtatcgc tgagcgcctg
     7321 caccggaatt ttctggaaca taggtatctc actcgtccac agttaattat ctgatcatca
     7381 tacaaattta tggtgatcgc tgcaatccgt cacgacaaaa tacttttcag caatgttagc
     7441 cagaaataca aaccgtcgca cgctggaacc tgagttgctg ctcgtcctgg cgcgatgtaa
     7501 tgtcaccagc ccgcctgcgc cttgtatccc atcctgaaaa acgtccccaa gactcccgac
     7561 gagatcctga gcagctgcta ataaatttcc ggattcggat cggtcttttc catttctagc
     7621 gatgccgcca gcaaggccag ccgcgccacg acgccgtaca tataaaaacg gttcggcgcc
     7681 gttgtccccg gcctggcct
//
LOCUS       DASRDH010000242         4602 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_935455,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000242 DASRDH010000000
VERSION     DASRDH010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 4602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4602
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_935455"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="VFA14_05635"
     CDS             <1..480
                     /locus_tag="VFA14_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HZS80891.1"
                     /translation="PRGDKRAYSDSALAELAKPDAATSTTMPADKPVVVGEDEAVSWM
                     WPADGKVVSAFGGGKRGIDIAGNSGQPVLAAAAGKVMYAGSGIRGYGNLVIVKHTNNL
                     LSAYAHNETILVKEGELVTKGQKIAGMGNSDSDSVKLHFEIRQQGKPVDPSKYLPSR"
     gene            484..1467
                     /gene="rpoS"
                     /locus_tag="VFA14_05640"
     CDS             484..1467
                     /gene="rpoS"
                     /locus_tag="VFA14_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007877260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HZS80892.1"
                     /translation="MRPRPHDRQPDNESRSNDPVEPLDAADDREVMKSVDGGGKGTKR
                     LKKSRAAELSTDITQHYLHQIGMRPLLTAAEELYFATLARQGDFTSRQKMIEHNLRLV
                     VSIAKFYINRGVALLDLIEEGNLGLMRAIDKFEPERGFRFSTYATWWIRQSIERAIMN
                     QARTVRLPVYMLRELNQILRAKYHLEAHGCDATVEDIAHLVNRPLEDVQDVLILSEHA
                     ASLDTPLDNDPQSSLIDLLACPQDGSPDTQAECHEMSILVHDWLNKLSDKQKTVITRR
                     FGLDNDDPSTLGQLAAEMGITHERVRQIQQEALIKLKRMLAARGVKKDALF"
     gene            1538..2896
                     /gene="rlmD"
                     /locus_tag="VFA14_05645"
     CDS             1538..2896
                     /gene="rlmD"
                     /locus_tag="VFA14_05645"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007877262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HZS80893.1"
                     /translation="MQQTIIDINALDMDGRGVGHLTNEDGSQGKVVFVEGALPGEQVS
                     FHSYRKKPKWEAATMTALHRESSLRVKPKCSYFGICGGCAMQHLEPSAQVAMKQRVLE
                     DNLWHLSKLKSEFVLRPIYGPTWSYRHRARISVRNVIKKGGVLVGFHEKSSSFVSDMK
                     TCEILPPHVSAMLVPLRRLIGSLSIIDHLPQIELAIGEDDGGKKNTVMVLRVMEALST
                     EDETKLKVFADEHEVEWWLQPKGPDTAYPYYPPQSSLRYTLPEFGIDIPFKPTDFTQV
                     NHQINRVLVAQVLRLLDAQPDDRVADLFCGLGNFTLPIATIVKEVVGIEGSTALTERA
                     AENARINKLVNKTSFHCRNLFAATTEDFIASGKFDRMLIDPPREGALEVCKAITGLGE
                     QYPHLKPKRIVYVSCNPATLARDAGVLVNLGSYRLKQAGVINMFPHTAHVESIALFDF
                     DA"
     gene            complement(2984..4159)
                     /locus_tag="VFA14_05650"
     CDS             complement(2984..4159)
                     /locus_tag="VFA14_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HZS80894.1"
                     /translation="MKRRKGSDTPTFDPAALPYETIALVLQGGGALGSYQAGVFQGLH
                     EAGIPLNWIAGISIGALNTALIAGNAPECRVEQLRAFWERICQPALFPTLPALFETAL
                     FNSTDQIRAVFTASQAMSAVMQGQRGFFVPRFPPPMSGVCERPEQVSWYDASPLRATL
                     LEFCDFDRINTGGIRVSVGAVNVGSGNFIYFDNTRMKLRPEHFMASGALPPAFAPVEI
                     DGEYYWDGGVVSNVPLLEVLGAQPRRDTLTFQVDLWRMRGPLPHSMADVAERQKDIQY
                     SSRTRFITDTMQREQCYRNTLHHLLEQIPDARRKSDPWCIAAEKLACSKKYNVQHLIY
                     EENACDQHYKDYQFGLSTMQGHWASGLADIRKMLAVKNGLAPPENGSGFVTHDIYRK"
     gene            complement(4156..>4602)
                     /locus_tag="VFA14_05655"
     CDS             complement(4156..>4602)
                     /locus_tag="VFA14_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZS80895.1"
                     /translation="HVDGAFLTTKAALKHMYKDERGGVVIYMGSVHSHEASPLKSAYV
                     AAKHALLGLARVLAKEGAKHNVRSHVICPGFVRTPLVDKQIPEQAKELGLSEEEVIKK
                     VMLGNTVDGVFTTVQDVAQTAVFLAPFPSAALTGQSFIVSHGWCMQ"
BASE COUNT         1059 a         1328 c         1276 g          939 t
ORIGIN      
        1 ccgcgcggcg acaaacgcgc ctattcggac agcgcgctgg ctgagctggc aaaaccggat
       61 gctgcaacca gtacaacaat gccagccgac aaacctgttg tggtcggtga ggacgaagcg
      121 gtatcgtgga tgtggccggc cgatggcaag gtggtcagcg cctttggcgg cggtaaaaga
      181 ggcatcgata ttgccggcaa cagcggacag ccggtgctgg cagcggctgc cggcaaggtc
      241 atgtatgccg gcagcggcat tcgtggttat ggcaatctag taatcgtcaa acatacgaac
      301 aatctcttgt cggcctacgc gcataacgag accatactgg taaaggaagg agaacttgtc
      361 actaagggac agaagattgc cgggatggga aattcggata gtgacagcgt caaattgcat
      421 ttcgaaatcc gccagcaagg taaacctgtc gatccgtcga aatatctacc gtcacgttag
      481 tgtatgcgcc cacgccctca cgatcgccag ccagacaatg aatcccgttc gaatgaccca
      541 gtcgagccac tggatgctgc cgacgaccga gaagtaatga agtccgtcga tggtggcggc
      601 aaaggcacga agcgcctgaa aaaatcccgt gctgccgaac tctccaccga catcacccaa
      661 cattatctcc atcaaatcgg catgcgacca ctactgacag ccgcagaaga actatacttc
      721 gcaacgctgg ccagacaggg cgattttacc tcgcgtcaaa aaatgatcga acacaacctg
      781 cgtctggtcg tgtcgattgc caagttttac atcaaccggg gcgtggcttt gctggacttg
      841 atcgaagagg gtaatctcgg ccttatgcgt gccatcgaca agtttgaacc cgagcgtggc
      901 ttccgtttct ccacctacgc tacctggtgg atacgtcaaa gcatcgagcg cgccatcatg
      961 aaccaggcgc gcacagtgcg tttgccagta tacatgttac gtgaactgaa ccagattctg
     1021 cgcgccaaat accatctgga agcacatggc tgtgacgcta cggtggaaga catcgcacat
     1081 ctggtgaacc ggccactcga agatgtgcag gacgtgttga ttttgtcaga acatgccgct
     1141 tcgcttgaca cgccgctcga caacgatcca cagtccagtc tgattgacct gttggcttgc
     1201 cctcaggatg gcagtcccga cacacaggcc gaatgtcatg agatgtccat actcgttcac
     1261 gactggctga acaaactgtc ggacaagcag aaaaccgtca tcacgcgccg cttcggtctc
     1321 gacaacgatg atccttccac actggggcag ctggctgccg agatgggcat cacccacgaa
     1381 cgcgtgcgtc agatccagca agaagcgctg atcaagctga aacgcatgct ggccgcacgc
     1441 ggcgtcaaga aagacgcatt gttttagatc cctgaatcac cgacaatagc ggttttgatt
     1501 tttccagccg cgacatcggt cgcatatatt cattttcatg cagcaaacca ttatcgatat
     1561 caacgcccta gacatggatg ggcgcggcgt cggtcatctg acaaatgaag atggcagtca
     1621 gggtaaggtc gtattcgtcg aaggcgcatt gccaggcgag caggtcagtt tccattccta
     1681 tcgcaaaaaa ccgaagtggg aagccgccac gatgacggcc ttgcatcgcg aatcatcgtt
     1741 acgcgtcaag ccaaaatgca gctacttcgg tatttgcggc ggctgcgcca tgcagcacct
     1801 agagccatcg gcccaagtcg ccatgaagca gcgcgtactg gaagataacc tttggcacct
     1861 cagcaagctc aagtcagaat tcgtgctgcg tccgatttac ggtccgacct ggagctatcg
     1921 ccatcgtgcg cgaatttcag tcaggaatgt catcaaaaaa ggcggggtgc tggttggttt
     1981 ccacgagaaa agctcatctt tcgtcagcga tatgaagacc tgcgaaatcc tgccgccgca
     2041 tgtttcggcc atgttggtgc cgctacgccg gttgatcggt tcactgtcga tcatcgatca
     2101 cttgccacaa atcgaactgg caatcggcga agatgatggc ggcaaaaaaa atacggtgat
     2161 ggtgttgcgc gtcatggaag cgctgagtac tgaagatgaa accaaactca aggtctttgc
     2221 tgatgagcat gaggtcgagt ggtggctgca accgaaaggg cctgatacgg cctaccccta
     2281 ctacccgcca caatcgagct tgcgttacac gctgccggaa ttcggtattg acataccgtt
     2341 caagccgacc gatttcacac aagtgaatca ccagatcaat cgtgtgttgg tggcacaggt
     2401 attgcgcttg ctggatgcgc aaccagacga ccgcgtggcc gacctgttct gtggccttgg
     2461 caatttcacc ttgcccatcg ccacgatagt gaaagaagtc gtcggcatcg aaggcagtac
     2521 ggccttgacc gaacgcgcag cagaaaacgc caggatcaac aagcttgtca acaaaacttc
     2581 gtttcattgc cgtaatctat tcgcagcgac gacggaagat ttcatcgcat caggcaagtt
     2641 cgaccgcatg ctgatcgatc cgccgcgtga aggtgcgctg gaggtctgca aagcgattac
     2701 cggtcttggc gagcaatacc cgcatctgaa acccaagcgt atcgtctatg tgtcatgtaa
     2761 tccagcgacg ctggcgcgtg acgctggcgt gctggtaaat ctgggtagct accgtctgaa
     2821 gcaggcgggc gtgatcaata tgttcccgca tacggcgcat gtagaatcga tagcgctgtt
     2881 tgacttcgat gcgtaaggcg tacctataga ccacgcctgg aatttgctat gcttccgttt
     2941 ctcgatttta cgaaacgttg gcatacactt ttttcaaccg aactcacttg cggtaaatgt
     3001 cgtgcgtgac gaaaccagag ccgttctctg gcggcgcaag gccattcttg accgcgagca
     3061 tcttgcggat gtctgcgagg ccggaggccc aatggccctg cattgtcgac aaaccgaact
     3121 ggtagtcctt gtagtgctgg tcgcaggcat tttcctcata gatcagatgc tgcacattgt
     3181 acttcttgct gcacgccagt ttctcggccg caatacacca cgggtcggac ttgcgccgtg
     3241 catccggaat ctgctcgagc aggtggtgca aggtattgcg gtagcactgc tcgcgctgca
     3301 tcgtgtcggt tatgaaacgc gtgcgacttg agtactggat gtccttctgg cgctccgcga
     3361 catcagccat cgaatgtggc aacggcccgc gcatgcgcca cagatcgacc tggaacgtga
     3421 gtgtatcccg gcgcggctgc gcgcccagca cttccagcag cggcacgttc gacacgacac
     3481 ccccgtccca gtaatactcg ccatcgatct caaccggcgc aaacgcgggc ggcagtgcgc
     3541 cggacgccat gaaatgctcg ggcctgagct tcatgcgcgt gttgtcgaaa tagatgaaat
     3601 tgccgctacc gacattgacc gcgccgaccg acacgcgtat cccgcccgta ttgatccggt
     3661 cgaagtcgca gaactcgagc agcgtggcgc gcagcggcga tgcgtcgtac cagctgacct
     3721 gttccggacg ttcgcagaca ccggacatcg gcggcggaaa gcgcggcacg aagaaccccc
     3781 gttgcccctg catcaccgcg ctcatggcct gcgatgcagt gaaaaccgca cggatttggt
     3841 cggtactgtt gaacagtgcc gtctcgaaca gcgccggtag cgtcgggaag agcgccggct
     3901 ggcaaatccg ctcccagaac gcacgcagtt gctcgacgcg gcattccggc gcattgccgg
     3961 cgatcaacgc ggtattgagt gcgccgatcg aaatgcccgc gatccagttc agcggaatgc
     4021 ccgcctcatg tagcccttgg aacacaccgg cctggtagga gccgagcgcg ccgccgccct
     4081 gtaacaccaa ggcgattgtt tcatagggca atgcagccgg atcgaaggtc ggagtatcgc
     4141 tacccttgcg ccgcttcatt gcatacacca gccgtggctg acgatgaacg actggccggt
     4201 cagcgcggcg ctcggaaacg gagccagaaa caccgccgtc tgcgcgacat cctgcacggt
     4261 tgtgaacacg ccatcgaccg tattgccaag catcaccttc ttaatcactt cctcttcgct
     4321 gaggccaagt tccttcgctt gctccgggat ctgcttgtcg accagcggcg tgcgcacgaa
     4381 gcccgggcag atgacatgcg agcgcacatt gtgcttggcg ccctccttgg caagcacgcg
     4441 cgccagaccc agcaaagcat gcttggcggc cacataggcc gatttcagcg gcgatgcctc
     4501 atgcgaatgc accgaaccca tgtagatcac gacaccacca cgctcgtcct tgtacatgtg
     4561 cttgagcgcg gccttggtcg tcaaaaacgc gccgtcgaca tg
//
LOCUS       DASRDH010000243         3181 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1305360,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000243 DASRDH010000000
VERSION     DASRDH010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 3181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3181
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1305360"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..148
                     /locus_tag="VFA14_05660"
     CDS             <1..148
                     /locus_tag="VFA14_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-((5-
                     phosphoribosylamino)methylideneamino)imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HZS80896.1"
                     /translation="YLYQLGRAQGAMWESYLQKLEAAGFTRESGRGRNLPIQGGSACC
                     RCHH"
     gene            215..949
                     /gene="ubiE"
                     /locus_tag="VFA14_05665"
     CDS             215..949
                     /gene="ubiE"
                     /locus_tag="VFA14_05665"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008332016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HZS80897.1"
                     /translation="MTNTTHFGYQTVSEKDKVRKVATVFHSVAAKYDIMNDFMSVGLH
                     RVWKAFTVVQAGIRPGFKVLDIAGGTGDLSKDFARQAGPGGEVWLTDINESMLRVGRD
                     RLLNKGIQAPTLLCDAEKLPFPDNYFDCVSVAFGLRNMTHKDVALVEMRRVLKPGGKL
                     LVLEFSRVWKPLQKPYDVYSFLVLPWLGKKIANDAASYRYLAESIRMHPDQETLKKMM
                     ESAGLERVQYYNLSAGVAALHTGIKL"
     gene            1024..1590
                     /locus_tag="VFA14_05670"
     CDS             1024..1590
                     /locus_tag="VFA14_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterol-binding protein"
                     /protein_id="HZS80898.1"
                     /translation="MRSAVAVINHLLAEESWAQELLVTHRAKTICIDGGPGRVFLTVT
                     GDGFLESAAVDAVPAVTIRFRLADLPLILLNRERAVSYMKIEGDADFANTISQLSQSL
                     RWDTEEDLSKIVGDIAATRIVASAKSMMAAMQTMQRQFAEHVAEYFLEEQPMLIRPHL
                     MTEFSSGVVKLRDDLERLSKRIEKLERR"
     gene            1590..3167
                     /gene="ubiB"
                     /locus_tag="VFA14_05675"
     CDS             1590..3167
                     /gene="ubiB"
                     /locus_tag="VFA14_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HZS80899.1"
                     /translation="MLLRSLRLFKIIRVAVRYGLDEIVVSGFDAPRILRLLNAVFFWR
                     DLSMPRGERLRRALEDLGPIFVKFGQVLSTRRDLLPPDIAEELTRLQDRVPPFDSNLA
                     VEQIQKSLGAHPDALFATFERAPVASASIAQVHLAALKDGTEVAVKVLRPGMRSAIDH
                     DIGLMYIAADWVERLWADGRRLKAREVVAEFEKYLHDELDLMREAANASQLRRNFANS
                     ALLMVPEMFWDYCSSSVIVMERMCGIPIGQTERLLAAGVDLGKLSRDGVEIFFTQVFR
                     DGFFHADMHPGNILVSIAPATFGRYIALDFGIVGTLTDFDKDYLSQNFLAFFRCDYKR
                     VAEAHIESGWAPKETRVDELEAAVRACCEPIFDRPLKDISFGQVLLRLFQTSRRFNVE
                     VQPQLVLFQKTLLNVEGLGRQLDPDLDLWKTAKPYLERWMSEQLGWRGFVQQLRIEMP
                     RYSRLLPQFPRLAHQALMMVDKENTQHTELLTKLVAEQRRTNMLLGMIVYFGGGLIGG
                     IVVVQLLLHFLRSYSVL"
BASE COUNT          633 a          817 c          927 g          804 t
ORIGIN      
        1 ctatctatat cagcttggcc gtgcccaagg ggcgatgtgg gaaagttatc tacaaaaact
       61 ggaagcggca gggtttacgc gtgaaagcgg tcgtggtcgg aacctgccca tccagggcgg
      121 cagtgcttgc tgccgttgtc accactgatt ccaccgcgat ttcattgcga tcctcacctt
      181 atataatatc gcatcattta tttcagcttg tttcatgacc aataccacgc attttggtta
      241 ccagactgtt tctgaaaaag acaaggttcg caaagtcgcc acagtctttc actccgtagc
      301 cgccaagtac gacatcatga acgacttcat gtcggtcggc ctgcatcgcg tgtggaaggc
      361 ttttacggtt gttcaggcgg gtattcgccc cggcttcaag gtactcgata ttgccggcgg
      421 caccggcgat ctgtccaagg acttcgccag acaggcaggc ccgggcggcg aggtctggct
      481 gaccgatatc aatgaatcga tgctgcgggt cggccgcgat cgcctgctga ataagggtat
      541 tcaggctccg accttgctgt gcgatgcgga aaaactgcca ttcccggaca actatttcga
      601 ctgcgtttcg gtggcttttg gtctgcgcaa catgacgcac aaggatgtgg cgctggtgga
      661 aatgcgtcgg gtactcaagc cgggcggtaa gttgctggtg ctggagtttt ccagggtatg
      721 gaagccgttg cagaagcctt atgatgttta ttcgtttttg gttctgccgt ggctgggcaa
      781 aaaaatcgct aatgatgccg caagctatcg ttatctggct gagtcgatcc gcatgcatcc
      841 ggatcaggaa accttgaaga agatgatgga aagtgccggt ctggagcgtg tgcagtacta
      901 taacctgagc gccggcgtgg ctgcattgca taccggtatc aagctttgac atcaagcgcc
      961 aatttgcgtc atttgtgcag gagcctgcta cacacggcga tttcttccat gttttcttcc
     1021 ttgatgcgtt ctgctgttgc tgttatcaat cacctgctcg ccgaggagtc atgggcgcag
     1081 gaactgttgg tgacgcatcg cgccaagact atctgtattg acggcgggcc ggggcgagta
     1141 tttctgacag tcaccggtga cggtttcctg gagagcgctg ccgttgatgc cgtgcctgcc
     1201 gtgaccatcc gtttcagatt ggccgatctg ccgctgatcc tgctcaatcg cgagcgtgcc
     1261 gtatcgtata tgaagattga aggggatgcc gattttgcca ataccatctc acaactgagc
     1321 cagtcgctgc gctgggatac cgaggaagat ctcagcaaga tcgttggcga catcgctgca
     1381 acgcgcatcg tcgccagcgc caaatcgatg atggccgcta tgcagactat gcaacggcag
     1441 ttcgccgaac atgtcgccga gtattttctg gaagaacaac cgatgttgat ccgccctcac
     1501 ctcatgaccg agttttccag cggtgtcgtc aagctgcgcg atgaccttga acgcttatcc
     1561 aagcggatag aaaagctgga acgtcgttga tgctgctgcg ttccctgcgt ctgttcaaga
     1621 tcatccgtgt ggcggtacgc tatggactgg atgaaatcgt tgtttcaggt tttgacgcgc
     1681 cgcgcattct ccgtcttctg aatgctgtat ttttctggcg cgatttgtca atgccgcgcg
     1741 gtgagcgtct gcgtcgcgca ctggaagatc ttggaccgat tttcgtcaag ttcggtcaag
     1801 tgttatcgac gcgtcgtgat ctgctgccgc cggatatcgc cgaagaactg accaggttgc
     1861 aagatcgtgt gccgccgttc gactccaacc tggcggttga acagattcag aaatcgctcg
     1921 gcgcgcatcc agatgccttg tttgcgacct tcgagcgtgc gccggttgcg tcggcgtcga
     1981 tcgcgcaggt ccacctggcg gcattgaagg atggcaccga agtcgccgtc aaagtcttac
     2041 gccccggcat gcgcagtgcc atcgaccatg acatcggcct catgtatatt gctgcagatt
     2101 gggtcgagcg cctgtgggct gatggccgcc ggctcaaggc cagggaagtc gtcgccgaat
     2161 tcgaaaagta tctgcatgat gagctggacc tgatgcgtga agccgccaac gccagccagt
     2221 tgcgtcgcaa tttcgccaat tccgcactac tgatggtgcc ggaaatgttt tgggattatt
     2281 gctcctcgtc cgtcatcgtc atggagcgca tgtgcggcat ccctatcggg cagactgaac
     2341 gtctgttggc tgcaggtgtg gatttgggca aactgtcgcg tgatggcgtt gagatttttt
     2401 ttacacaagt gttccgcgat ggtttcttcc acgccgacat gcatcccggg aatattctgg
     2461 tgtcgatcgc gccggctact ttcggtcgtt acattgcgct ggatttcggg atcgtcggta
     2521 cgttgacgga tttcgataag gattatttgt cgcagaactt cctggcgttc ttccggtgcg
     2581 actacaagcg tgtggccgaa gcccacatag aatccggctg ggcgccgaaa gagacacggg
     2641 tggatgagtt ggaagctgcc gtgcgtgcct gctgtgaacc gatcttcgat cgtcctttga
     2701 aggatatttc cttcggtcag gtattgttgc gtctgttcca gacatcgcgc cgcttcaatg
     2761 tcgaagtaca gccacaactg gtcttgttcc aaaaaacttt gctcaacgtc gaaggtctgg
     2821 gacgtcagct cgacccggat ctcgatctct ggaaaactgc caagccttat ctggagcgct
     2881 ggatgagtga gcagctcggc tggcgtggat tcgtacagca gctgaggatc gaaatgccgc
     2941 gttatagccg tctattgccg cagtttccgc gtctggcgca tcaggcgttg atgatggtgg
     3001 ataaggaaaa tacgcaacat accgagttgc tgacgaagct cgtggccgag cagcgtcgca
     3061 ccaacatgct gttggggatg attgtctact tcggtggggg attgatcggc ggaatcgtcg
     3121 tggtgcagtt actgctgcat ttcctgcgta gctattccgt gttgtgaaga cggtcgagat
     3181 c
//
LOCUS       DASRDH010000244         4257 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_985985,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000244 DASRDH010000000
VERSION     DASRDH010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 4257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4257
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_985985"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="VFA14_05680"
     CDS             <1..310
                     /locus_tag="VFA14_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HZS80900.1"
                     /translation="EAILNGRKVDLVLSDMAPNLSGVAIADAARVEHLVDLAIGFAQM
                     HMKPTGSLLVKCFNGATYNELLRKFRSEFKTVMPKKPKASRDKSSEIFLLGRILENPQ
                     "
     gene            597..2480
                     /gene="ftsH"
                     /locus_tag="VFA14_05685"
     CDS             597..2480
                     /gene="ftsH"
                     /locus_tag="VFA14_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008326840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HZS80901.1"
                     /translation="MNNMFSRVAMWGMIALVLFMVFKQFDKSNLASGAAPIAYSDFIN
                     EVKAGHIKDAVIEDRSIVATTQDGKKVKTAATYLDRGLVGDLLNNGVKFDVKQLEEQS
                     FLSQVFISWFPMLLLIGVWVFFMRQMQGGGKGGAFSFGKSKARMLDENSNSVTFSDVA
                     GCDEAKEEVQELVEFLRDPTKFQKLGGRIPHGVLMVGPPGTGKTLLARAIAGEAKVPF
                     FTISGSDFVEMFVGVGASRVRDMFENAKKHAPCIIFIDEIDAVGRHRGAGMGGGNDER
                     EQTLNQMLVEMDGFEVNSGVIVIAATNRADVLDKALLRPGRFDRQVMVGLPDIRGREQ
                     ILYVHMRKVPIAPDVKADILARGTPGFSGADLANLVNEAALFAARRGKRLVEMQDFED
                     AKDKIVMGPERKSAVMREEERRNTAYHESGHAVVAKLLPKADPVHKVTIMPRGYALGL
                     TWQLPEHDRINMYKDKMLEEIAILFGGRIAEEIFMHQMSTGASNDFERATKLARAMVT
                     RYGMSESLGTMVYEDTEQDAYFGRMSAKTVSEATQQKVDTEIRRILDTQYALSRRLLE
                     ENRDKVEVMAQKLLEWETIDADQISAIMAGNEPHPPKAGIPVRKSSGNTPGDMSPTAT
                     APA"
     gene            2614..3459
                     /gene="folP"
                     /locus_tag="VFA14_05690"
     CDS             2614..3459
                     /gene="folP"
                     /locus_tag="VFA14_05690"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HZS80902.1"
                     /translation="MSQRYLQCGRYLLPVCPGGRPLVMGILNVTPDSFSDGGSYASLE
                     FALSRAEQMIADGVDIIDIGGESSRPGTAPVSLEEELKRVMPVVYALRDCGKPLSIDT
                     CKPEVMREALIAGADMINDIHGFRAPGAIEAVKDSSAALCIMHMHGEPQTMQQQPVYG
                     DVVQEVALFLCERVRTIEAGGVERIRLCIDPGFGFGKSVEHNYALLRNTRRLIQELGL
                     PLLAGMSRKSMIGALTGKPLEQRLAGSLGAALAAAAQGAMLLRVHDVAETVDALEVWH
                     AGTAD"
     gene            3497..>4257
                     /gene="glmM"
                     /locus_tag="VFA14_05695"
     CDS             3497..>4257
                     /gene="glmM"
                     /locus_tag="VFA14_05695"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013233406.1"
                     /note="catalyzes the conversion of glucosamine-6-phosphate
                     to glucosamine-1-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HZS80903.1"
                     /translation="MAAKYFGTDGVRGCVGTMPITPDFVMRLGYAAGKVLTRSWASSV
                     KSTVLIGKDTRISGYMLEAALEAGFAAAGVDVMLAGPMPTPAIAYLTRALRLSAGVVI
                     SASHNPYHDNGIKFFSASGNKLPDAVEADIEAELNLPMHCVSSEKLGKAKRLDDAQGR
                     YIEFCKSTFPNDLDLHGMRIVVDCAHGAAYNIAPHVFHELGAEVIDIGNQPNGLNINE
                     GCGAAAPEALSSSVRANHADIGIALDGDADRLVMV"
BASE COUNT          898 a         1143 c         1239 g          977 t
ORIGIN      
        1 ggaagccatt ctgaatggcc gcaaggtcga tctggtgctg tccgacatgg cgcccaacct
       61 gtccggcgtg gctattgccg atgcggcgcg tgtcgagcat cttgtcgatt tggcaattgg
      121 attcgcgcaa atgcacatga aaccgaccgg ctcattactc gtcaaatgtt tcaatggcgc
      181 cacttacaac gaactgttgc gtaagttccg ctccgaattc aagacggtga tgccgaagaa
      241 gccgaaggct agccgggaca agtcgtctga aattttcctg ctaggcagga ttctggaaaa
      301 tccgcagtga acccatgtcg cgttgcgtat cttgcacaat caaccagcgt ttttcctgtt
      361 tctctccttg atttcacgtt atctgctcat acatcagtca taggaaacca tccccgtcct
      421 gtctgaaagg catttttttg ccgatttttc gcactcaggt tttcccgttt gctggccgct
      481 atccaaaaca gtgctccagt atgggtagtt tcctgcaagc gcgggtgaat ccgggtagaa
      541 tcagatactt atattgagat aacaggtaga ggcgcgctcg cgtcagagga gttccagtga
      601 acaatatgtt ttccagagtc gccatgtggg ggatgatcgc cctggtgctg tttatggtct
      661 ttaaacagtt cgacaaaagc aatctggcgt caggcgcggc accgattgcc tactccgatt
      721 tcatcaatga agtaaaagcc ggccacatca aggacgcggt catcgaggat cgtagtatcg
      781 tcgcgacgac gcaggatggc aagaaagtca agaccgcagc cacctatctg gatcgcggcc
      841 tggtcggcga tctgctcaat aatggcgtca agttcgacgt caagcagctc gaagagcaat
      901 ccttcctgtc acaggttttc atttcctggt tcccgatgct gttgctgatc ggtgtctggg
      961 tatttttcat gcgccagatg cagggcggtg gtaagggcgg cgctttttcc ttcggcaagt
     1021 cgaaggcgcg catgctcgat gaaaacagca attccgtcac tttctccgac gtcgccggtt
     1081 gtgatgaggc gaaagaagaa gtgcaggagc tggttgaatt tctacgcgat ccaaccaaat
     1141 tccagaaact cggtggtcgc attccacacg gcgtactgat ggtcggtccc ccgggtaccg
     1201 gtaagacatt gctggcgcgc gccattgccg gcgaagccaa agtgccattc tttaccatct
     1261 ccggttccga tttcgtcgaa atgtttgttg gcgtaggcgc atcgcgcgtg cgcgacatgt
     1321 ttgaaaatgc caaaaaacat gcgccgtgca tcattttcat cgacgaaatc gatgccgtcg
     1381 gtcgccatcg cggcgctggc atgggcggag gcaacgatga gcgtgaacag accttgaacc
     1441 agatgctggt cgaaatggac ggtttcgaag tcaattccgg cgtcatcgtg atcgctgcca
     1501 ccaaccgtgc cgatgtactc gacaaggcgc tgttgcgtcc gggccgtttc gaccgtcagg
     1561 tcatggttgg cctgccggac attcgcggtc gtgagcagat tctttacgtc cacatgcgca
     1621 aggtgcctat cgctcctgat gtcaaagccg acatcctggc gcgtggtacg ccgggtttct
     1681 ccggcgccga cctggcgaat ctggtcaacg aagccgccct gtttgcggcg cgtcgcggca
     1741 agcggctggt ggaaatgcag gacttcgaag atgccaagga caagatcgtc atgggaccag
     1801 agcgcaagtc tgccgtgatg cgtgaagaag agcgccgcaa caccgcttat cacgaatccg
     1861 ggcatgccgt tgtcgccaaa ctgctgccga aggctgatcc cgtgcataag gtcaccatca
     1921 tgccgcgcgg ctatgcgctc ggtctgacct ggcagttgcc cgaacatgat cgcatcaaca
     1981 tgtacaagga caagatgctg gaagaaatcg ctatcctgtt tggtggccgc atcgcggaag
     2041 aaatttttat gcatcagatg tcgaccggcg cgtccaacga tttcgagcgc gccaccaaac
     2101 tggcgcgtgc catggtgacg cgttatggca tgtctgaatc gctgggcacc atggtctatg
     2161 aagacaccga gcaggacgcc tacttcggtc gcatgtctgc caagaccgtg tcggaagcca
     2221 cccagcaaaa ggtcgatacg gaaatccgcc gcatcctcga cacgcaatac gcgctctcgc
     2281 gtcgtcttct ggaagagaat cgtgacaagg tcgaagtcat ggcgcaaaaa ctgctcgaat
     2341 gggaaaccat cgatgctgac cagatcagcg ctatcatggc aggcaatgaa ccgcatccgc
     2401 cgaaggcggg catcccggtc aggaaatctt ccggcaacac gccgggcgat atgtcgccaa
     2461 ccgctactgc gcctgcttga ctacagcgct gaaggtgaag ctggtttcag ggtgaggagt
     2521 gatcctcacc ctttttcttg gtgctttgtc gctgatcgac ggcaggccgt tttcattgca
     2581 tcaggcgttc ctggtttgaa atgatcaagc agaatgagcc aacgatattt acagtgtgga
     2641 cgttacctcc ttcccgtgtg ccctggcggc aggccgctgg tcatgggtat tctcaatgtc
     2701 acgcccgatt cgttttctga cggcggcagt tatgcttcac ttgagtttgc cttgtcgcgc
     2761 gccgagcaga tgattgctga tggtgtcgat atcattgaca tcggcggtga gtccagtcgg
     2821 cccggcactg cgccggtctc gctggaggaa gaactcaagc gcgtcatgcc ggtggtatat
     2881 gccttgcgcg attgtggcaa gcctttgtcg atcgacacct gtaaaccgga agtcatgcgc
     2941 gaagcgctga tcgccggtgc tgacatgatc aacgacattc atggttttcg cgcccctggc
     3001 gctatcgaag ccgtcaagga tagcagcgcc gctctgtgca tcatgcacat gcatggcgaa
     3061 ccgcagacca tgcagcagca gccggtctat ggcgatgtcg tgcaggaagt cgcgctgttt
     3121 ttgtgtgagc gcgttcgcac catcgaggct ggcggtgtcg aacgtattcg tctgtgcatc
     3181 gatccgggtt ttggtttcgg gaagagcgtc gaacacaatt acgccttgct caggaataca
     3241 cgccgtttga ttcaggaact cggtttgccg ctgctggcag ggatgtcgcg caaatcgatg
     3301 atcggcgcgc ttaccggcaa gccgctggag cagcgtctgg cgggcagtct tggcgcagct
     3361 ttggcagcgg ctgcgcaagg cgcgatgctg cttcgcgtgc atgacgtcgc ggagactgtc
     3421 gatgccctcg aggtctggca tgccggcacg gcagattaaa ctattcatca agatcaagag
     3481 aaggctagaa aatcatatgg ctgcaaaata tttcggtact gatggcgtgc gtggatgcgt
     3541 ggggaccatg ccgatcacgc ctgatttcgt catgcgtctc ggctatgctg ctggtaaggt
     3601 gctgaccagg tcatgggcat cgtccgtcaa atcaacggta ctgataggca aggacacgag
     3661 gatttccggt tacatgctag aagccgccct ggaggcggga tttgccgcag ccggtgtgga
     3721 tgtgatgctg gccggcccga tgccgacgcc ggccattgct tatctcacgc gcgccttgcg
     3781 cctgtcggca ggagtggtga tttcggcttc gcataatccg tatcatgaca atggcatcaa
     3841 atttttctcg gcatctggca acaagttgcc agatgccgtg gaggctgaca tcgaggctga
     3901 actgaatctt cccatgcact gcgtttcatc ggagaagctg ggcaaggcaa agcgcctaga
     3961 tgatgctcag ggacgttaca tcgaattttg caagagcact tttcccaatg acttggactt
     4021 gcacggcatg cgcatcgtgg tcgattgcgc gcatggcgca gcctataaca tcgcgccgca
     4081 tgtgtttcat gaactgggtg ccgaagtcat cgacatcggc aatcagccca acggcctgaa
     4141 catcaacgag ggctgcggcg cggccgcacc ggaagccttg tccagttcgg tgcgtgccaa
     4201 tcatgccgat atcggtattg ccctcgatgg tgacgccgat cgtctggtca tggtgga
//
LOCUS       DASRDH010000245         2127 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_450920,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000245 DASRDH010000000
VERSION     DASRDH010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450920"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="VFA14_05700"
     CDS             <1..197
                     /locus_tag="VFA14_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007879881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="surface-adhesin E family protein"
                     /protein_id="HZS80904.1"
                     /translation="EVSIAYFDCEKKRIGFGSRTMYSKPNAQGEVLLSPSLDYSNVKL
                     QDVEADSTGAQMLKAICATK"
     gene            274..358
                     /locus_tag="VFA14_05705"
     tRNA            274..358
                     /locus_tag="VFA14_05705"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:308..310,aa:Leu,seq:caa)
     gene            554..1363
                     /locus_tag="VFA14_05710"
     CDS             554..1363
                     /locus_tag="VFA14_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZS80905.1"
                     /translation="MSLTTSLPPAGAHGIKLEAVTLRRGDRIVLEQIDLHLTQSRIGL
                     IGDNGAGKSSLFRLICGLDQPQAGRVAVHGCDTADAAQRRTLPQQVGMMFQNPEDQII
                     FPTVAEELAFSLTAAGIGKAQARQQAQAFLTQRGWADWGPRAIGELSQGQRQQVCLMA
                     LQITSPATLLLDEPYSALDLPSQLRLSAQIAATDQQIILSTHLLDHVCDFERVLWLDS
                     GKLRGDGPGRDICAAYAQDVRKRSAAAVAAKLLERIHHLTISPHVALGNDA"
     gene            1356..1949
                     /locus_tag="VFA14_05715"
     CDS             1356..1949
                     /locus_tag="VFA14_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HZS80906.1"
                     /translation="MRSLYIARRTWLHAIPASIKLIALSIAGTALILSGTSSHLAIAA
                     AVVLALFLSLGTAGWQQWRMLRSLLIAAALIAGFHWLMGDPAIGLSSALRIMAASMLA
                     LMLTITTRFDDLLAVLEYILSPLRRVGVPVERIALGIGLTLRFVENFYAQWQRLNDAH
                     RARTGRNGGWRLLAPLAIRALQTAERVADALTTRLGN"
     gene            1975..>2127
                     /locus_tag="VFA14_05720"
     CDS             1975..>2127
                     /locus_tag="VFA14_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BioY family transporter"
                     /protein_id="HZS80907.1"
                     /translation="MTTGPMTSTRSLAHIALFAAVIAVFGLIPRVELPFGVPITLQTL
                     GIMLVGC"
BASE COUNT          414 a          695 c          602 g          416 t
ORIGIN      
        1 ccgaagtatc gatcgcttac ttcgattgcg aaaaaaaacg catcggtttc ggtagccgaa
       61 ccatgtattc caagccgaat gcccaaggcg aagtgctgct ctccccttcc ctcgactatt
      121 ccaacgtcaa gctgcaagac gtggaagccg actcgaccgg cgcgcaaatg ctcaaggcca
      181 tttgcgcgac aaaataaagt cggagcagct tctcttgcca aaaggaggtg ttgcccggct
      241 tcctggaata agtcataatc ccggtcctgc gttgccggga tggcggaaca ggtagacgca
      301 cgggactcaa aatcccgcgc cgcaaggcgt gccggttcga ttccggctcc cggcaccaga
      361 gttgcagaca aaaaccctcg tttcctggca tgactatgaa ccttcctccc gattgctcaa
      421 tcaaagcttt cgccactgcc agtccgtagc ctgaatgccc tttccgggct gaccagtcgc
      481 gcttacaggt ttcagggcac gctacagctg ccatattaat gacggcccca caacaggcgc
      541 aagccgacac gacatgagcc tcaccacatc gttgcccccc gccggcgcgc acggcatcaa
      601 actggaggcg gtcacgctca gacgcggcga ccgcatcgta ctcgaacaga tcgatctgca
      661 cctgacgcag tcgcgcatcg gcctgatcgg cgacaacggc gccggcaaga gcagtctgtt
      721 tcgcctgatc tgtggactgg accagccgca ggccggacgt gtggccgtgc atggctgcga
      781 cactgccgac gcagcgcaac gccgcacgct gccacagcaa gtcggcatga tgttccagaa
      841 tcccgaagac cagatcattt ttccgacggt ggcggaagaa ctggcgttca gcctcaccgc
      901 cgccggtatc ggtaaagcac aggcgcggca acaggcgcag gcttttctga cacaacgcgg
      961 atgggcggac tggggtccgc gcgccatcgg cgaactgagc cagggccagc gccagcaagt
     1021 ttgtctgatg gcgctacaaa tcaccagccc tgccacatta ctgctggacg agccttattc
     1081 cgcactcgac ctgccaagcc agttgcgact atcggcgcag atcgccgcca ctgaccagca
     1141 aatcattttg tctacgcatc tgctggacca tgtctgtgat ttcgaacgcg tgctgtggct
     1201 ggacagcggc aagctgcgcg gtgacggtcc cggccgtgac atctgcgcag cgtatgcgca
     1261 ggacgtccgc aaacgcagtg ccgccgcagt agccgccaag ctcctggaac gtattcacca
     1321 tctcaccatc tcaccacacg tcgcgctggg aaacgatgcg tagcctgtat atcgcacgcc
     1381 gcacctggct gcacgccata ccggccagca tcaagctaat cgcgctctcc atcgccggca
     1441 cggcgctgat actgtccggt acgtcgtcgc atctcgccat cgctgctgcc gtggtgctgg
     1501 cgctgtttct ctcgctcggc acggccggct ggcaacagtg gcgcatgctg cgcagcctgc
     1561 tgatcgcagc ggcactgatt gccggcttcc attggctgat gggcgatcca gccatcgggt
     1621 tgagcagcgc cttgcgcatc atggcagcgt cgatgctggc gctgatgctg accatcacta
     1681 cccgcttcga tgatctgctg gcggtactgg aatatatatt gtcgccattg cgccgcgtcg
     1741 gcgtgccggt ggagcgtatc gcactgggca tcggtctgac gctgcgtttt gtagaaaatt
     1801 tttatgcgca atggcagcgt ctgaacgatg cccatcgcgc ccgcaccggc cgtaacggcg
     1861 gctggcgcct gctggcgccg ctggcgatac gcgccttgca aaccgctgaa cgcgtggctg
     1921 acgctctcac gacccgtctc ggcaactaga ttttcaatga aagatcgccc catcatgaca
     1981 accggcccca tgacctctac ccgctcgctt gctcatatcg ccctgtttgc cgccgtgatc
     2041 gccgtgttcg gcctgatccc cagggtcgag ctgccgtttg gtgtgccgat cacgctgcaa
     2101 acgctgggca tcatgctggt cggctgt
//
LOCUS       DASRDH010000246         4723 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1460765,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000246 DASRDH010000000
VERSION     DASRDH010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 4723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4723
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1460765"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..658
                     /locus_tag="VFA14_05725"
     CDS             <1..658
                     /locus_tag="VFA14_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013392146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HZS80908.1"
                     /translation="INNTETFAAVPFIFRIGAQAYAAKGKPNNGGTKIFSVSGDVECP
                     GNYEIPLGTPFSTLLELAGGMRGGRKIKAVIPGGSSAPVIRGDVMLDISMDYDAIAKA
                     GSMLGSGAVIVMDETRCMVKSLLRLSYFYYEESCGQCTPCREGTGWMYRMVERIEDGH
                     GRPEDLDVLNSVADNIQGRTICALGDAAAMPVRAFIKNYREEFEYHIEHRQCLVPAYV
                     "
     gene            743..3070
                     /gene="nuoG"
                     /locus_tag="VFA14_05730"
     CDS             743..3070
                     /gene="nuoG"
                     /locus_tag="VFA14_05730"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013233779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HZS80909.1"
                     /translation="MVEIEIDGKKVAVQEGSMVMDAASKVGIYIPHFCYHKKLSIAAN
                     CRMCLVDIEKAPKPLPACATPVSAGMILRTASDKAVKAQNGVMEFLLINHPLDCPICD
                     QGGECQLQDLAVGYGQSSSRYQEEKRVVPPKDIGPLISMQEMNRCIYCTRCVRFGQEV
                     AGVMELGMLGRGEHSEIATFVGKTVDSELSGNMIDLCPVGALTSRPFRYAARTWELSR
                     RKSISPHDSLGANLIVQVKGGKVMRVLPLENEAVNECWISDKDRFAYEALNSEERLTR
                     PMLKQDGKWQEVEWQVALEYVAHGLRNIRHEHGANAIAALGTPYSTLEELGLLQKIVR
                     GMGSENIDFRLRQSDFALDRQIHPWLGMSIAELSQLNRAFIIGSFLRKDHPLLAVRLR
                     QAVKSGAKVSILHASDDDLLMPIASKIIAAPSAWLSVLGEVVVAVAQAKGVAVPAGFD
                     GVQVSEAARQTAAGLLSGESRAVLLGNAAAQHPQASQLHAAAQWIAGQTGARFGYLTE
                     GGNAVGGYLANAIPGKGGANALQAFAQPHKAYLLLNAEPELDSYDPQAAAAALKQADM
                     VVVMSAYRHGLDYADVLLPIAPFTETSGTFVNAEGRAQSFNGVVKPLGDTRPAWKVLR
                     VLGNLLELPHFDYDTSEAIRSEIIGVETSLAARLDNFAEAALQAAAAAVDGALERVAD
                     VPIHFADAIARRSPALLQTQDGRAPHARLSPALSQRFGIAAGDRVRVRQGQGSAVLTA
                     EIDAALPENVVRVAAAHVSTAGLGGMFGAIVVEKA"
     gene            3070..4149
                     /gene="nuoH"
                     /locus_tag="VFA14_05735"
     CDS             3070..4149
                     /gene="nuoH"
                     /locus_tag="VFA14_05735"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HZS80910.1"
                     /translation="MDQLIADINAAGSGYLGAAWPYVWTFVWSLLKILVVMLPLLLCV
                     AYLTYWERRLIGWMHVRLGPNRVGPAGLLQPIADALKLIFKEITTPARADKVLFFVAP
                     VMTIMPALAAWAVIPFGPEAVIANVNAGLLYVMAITSMEIYGIIIAGWASNSKYAFLG
                     AMRASAQMVSYEIAMGFCLVVVLMVSGSLNLIDIVTGQSRGYFADHGLGFLSWNWLSL
                     LPVFVIYVISGIAETNRHPFDAVEGESEIVAGHMIEYSGMSFAMFFLAEYANMILISM
                     LASLLFLGGWAAPVQLLDFIPGWIWLGIKTFLVVSLFVWVRASFPRYRYDQIMRLGWK
                     IFIPLTLAYLVIVATWIQTPWNIWK"
     gene            4173..4661
                     /gene="nuoI"
                     /locus_tag="VFA14_05740"
     CDS             4173..4661
                     /gene="nuoI"
                     /locus_tag="VFA14_05740"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HZS80911.1"
                     /translation="MQAIKDFFGSLMLRELFKGLALTGRYLFMRKITIQFPEEKTPQS
                     PRFRGLHALRRYPNGEERCIACKLCEAVCPAMAITIESEQRDDGSRRTTRYDIDLTKC
                     IFCGFCEESCPVDSIVETHILEYHGEKRGDLYYTKDMLLAIGDRYENEIAAARAADAA
                     YR"
BASE COUNT          934 a         1311 c         1438 g         1040 t
ORIGIN      
        1 gatcaataac accgaaacct tcgctgccgt gccgttcatc ttcaggatcg gtgcgcaggc
       61 ttatgcggcg aagggtaagc cgaacaacgg cggcaccaag attttttcgg tctcgggtga
      121 cgtcgaatgt ccgggcaact atgaaattcc gctcggcaca ccattttcca ccctgctgga
      181 actggctggc ggcatgcgcg gcggcagaaa aatcaaggcc gtcattcccg gtggttcgtc
      241 ggcgccggtg attcgtggcg atgtcatgct ggacatcagc atggactacg atgccatcgc
      301 caaggccggc tcgatgctgg gctcgggtgc cgtcatcgtc atggacgaga cccgctgcat
      361 ggtcaagtcg ctgctgcgcc tgtcctattt ttattacgaa gaatcctgcg gtcaatgcac
      421 gccatgccgt gaaggcaccg gctggatgta tcgcatggtc gaacgcatcg aagacggtca
      481 tggcaggccg gaagacctgg atgtgctcaa ttcggttgct gacaatattc agggtcgtac
      541 catctgcgcg ttaggcgatg cggcggcgat gccggtgcgt gccttcatca agaactaccg
      601 cgaagaattt gagtaccata tcgaacacag acaatgcctg gtgccggctt atgtttaacc
      661 tgagctgaaa gcggcatgac atgcgagcgg tggctcacat gtcatcggag tcaaaagaat
      721 atttagcgtg ggcgacaaag ccatggttga aatcgaaata gacggcaaaa aagtggcagt
      781 acaggaaggc agcatggtga tggatgccgc cagcaaggtc ggcatttaca ttcctcattt
      841 ctgctatcac aaaaagctgt ccattgcggc caactgccgt atgtgtctgg tggacataga
      901 aaaggcaccg aagcctttgc ctgcctgcgc gacgccggtc agcgccggca tgatcttgcg
      961 caccgccagc gacaaggcgg tcaaggcaca aaatggcgtc atggaattcc tgctgatcaa
     1021 tcacccactg gactgcccga tctgcgatca gggaggagag tgccagttgc aggatctggc
     1081 agttggctac ggtcagtcct catcccgcta tcaggaagaa aagcgcgttg tgccgccgaa
     1141 ggatatcggc ccgctgatct ccatgcagga aatgaaccgt tgcatctatt gcacacgttg
     1201 cgtgcgtttc ggccaggagg tggccggcgt catggagctg ggcatgctgg gtcgcggcga
     1261 gcactctgaa atcgccacat tcgtcggcaa gaccgtcgat tccgaactgt ccggcaacat
     1321 gatcgatcta tgtccggtcg gcgcattgac atccaggcca ttccgctacg ctgcgcgtac
     1381 ctgggaatta tcgcgccgca agtcgatcag tccgcatgac agcctcggcg ccaatctgat
     1441 cgttcaggtc aagggcggca aggtcatgcg cgtgttgccg ctggaaaacg aagcagtcaa
     1501 cgagtgctgg atttccgaca aggaccgttt tgcctacgaa gcgctcaaca gcgaagagcg
     1561 tctgaccagg ccgatgctga agcaggacgg caaatggcag gaagtcgagt ggcaggtcgc
     1621 gctggaatac gtcgcgcacg gcctgcgtaa catccgtcac gaacatggcg ccaatgccat
     1681 cgctgcgctg ggtacgccgt actcgacgct ggaagaactg ggattactgc aaaaaatcgt
     1741 gcgcggcatg ggttctgaaa acatcgattt ccgcctgcgt cagtccgact tcgccctgga
     1801 tcgccagatc catccatggc tgggcatgtc gatcgctgaa ttgtcgcaac tgaaccgcgc
     1861 cttcatcatc ggctcgttcc tgcgcaagga tcatccgctg ctggccgtgc gtctgcgtca
     1921 ggccgtcaag agcggcgcca aggtcagcat cctgcatgcg agcgacgacg atctgctgat
     1981 gccgattgcc agcaagatca ttgctgcacc gtccgcatgg ctgtctgtat tgggtgaagt
     2041 ggtggttgcc gtggcgcagg ccaagggcgt tgccgtaccg gccggtttcg atggcgtaca
     2101 ggtatcggaa gcggccaggc agaccgccgc cggtctgttg tcaggcgaat cccgggctgt
     2161 cctgctgggc aacgctgcgg cacagcatcc gcaagcctcg caactgcacg ctgccgcaca
     2221 atggatcgcc ggacaaaccg gcgccaggtt cggttacctg acggaagggg gcaatgccgt
     2281 cggcggttac ctggccaatg cgattccagg taaaggtggc gccaacgcct tgcaggcttt
     2341 cgcgcaaccg cacaaggcgt atctgctgct caatgccgag cctgaactcg acagctacga
     2401 tccgcaggct gccgctgctg cgctgaagca ggccgacatg gtcgtagtga tgtccgccta
     2461 taggcacggt ctggactacg ccgatgttct gctgccgatt gcgccgttca ccgaaacttc
     2521 cggcactttc gtcaacgccg aaggccgcgc gcaaagcttc aacggtgtcg tcaaaccgct
     2581 gggtgacacc cgcccggcgt ggaaggtgct gcgtgtgctg ggcaatctgc tggagttgcc
     2641 gcatttcgat tacgacacat cggaagcgat ccgcagcgaa atcatcggcg ttgaaaccag
     2701 tctggctgcg cgtctggata acttcgccga agcagcgctg caagcggctg ccgctgctgt
     2761 cgatggcgcg ctggaacgcg tcgctgatgt gcctatccat tttgccgacg ccatcgcgcg
     2821 ccgttcgccg gcgttgttgc agacacagga cggtcgggcg ccccatgccc ggttgtcgcc
     2881 tgccctgtca caacgattcg gtatcgccgc aggcgatcgt gtgcgcgtca ggcaagggca
     2941 gggcagcgcc gtattgaccg ctgaaatcga tgcggctttg ccggaaaatg tcgtccgtgt
     3001 ggcggccgcg catgtgagta ccgccgggct gggtggtatg ttcggcgcca tcgtcgtgga
     3061 gaaagcgtaa tggatcaact gattgccgat atcaatgcag caggttccgg ctatctggga
     3121 gcggcatggc cgtatgtgtg gactttcgtc tggagtctgc tcaagattct ggtggtgatg
     3181 ctgccattgc tactgtgcgt ggcttacctg acttattggg aacgcaggct gatcggttgg
     3241 atgcatgtgc gtctggggcc taaccgtgtc ggccctgccg gtctgctgca accgatcgct
     3301 gatgccctga agctgatttt caaggagatc acgacgccag cgcgtgccga caaggtgctg
     3361 ttcttcgttg cgccggtcat gaccatcatg ccggcactgg ccgcctgggc ggtgattccg
     3421 tttggaccgg aagctgtcat tgcgaacgtc aacgcgggtc tgctgtatgt gatggcgatc
     3481 acctccatgg agatttacgg catcatcatc gccggctggg catccaactc gaaatacgcc
     3541 ttcctcggcg ccatgcgcgc atcggcgcag atggtatcct acgaaattgc gatgggcttt
     3601 tgcctggtcg tggtgctgat ggtatccggc agcctgaatc tgatcgatat cgtcacgggc
     3661 caaagcaggg gttacttcgc cgatcacggt ctgggttttc tgtcgtggaa ctggctgtcg
     3721 ctgctgccgg ttttcgtgat ttatgtcatc tcgggtatcg ctgaaaccaa ccgtcatcca
     3781 ttcgatgcgg tcgagggcga atcggaaatc gttgccggtc atatgatcga atactcgggg
     3841 atgtctttcg ccatgttctt cctggctgaa tatgccaaca tgatcctgat ttcgatgctg
     3901 gcgtcgctgt tgttcctggg cggctgggca gcgccggtcc agcttctgga tttcatcccg
     3961 ggctggatct ggctcgggat caagaccttc ctggtagtgt cgctgttcgt ctgggtgcgc
     4021 gcgtcgttcc cacgctatcg ttatgaccag atcatgcgtc tgggttggaa gattttcatt
     4081 ccactgacct tggcgtatct ggtgatcgtc gcgacctgga tacagacccc gtggaatata
     4141 tggaaatgaa tgtggtcgag atgaaattaa agatgcaagc tatcaaggat tttttcggca
     4201 gcttgatgct cagggagctg ttcaagggcc tcgccctgac tggccgctat ttgttcatgc
     4261 gcaagatcac cattcagttc ccggaagaga agacgccgca gtcgccgcgt tttcgcggtc
     4321 tgcacgccct gcgccgctat ccgaatggcg aagagcgctg catcgcctgc aaactatgcg
     4381 aggcagtctg tccggccatg gccattacca tcgagtcgga acagcgtgat gacggttcgc
     4441 gccgtacgac gcgttacgac atcgatctga ccaaatgtat tttctgcggt ttctgcgaag
     4501 agtcttgtcc ggtcgattcc atcgtcgaaa cgcatattct cgaatatcac ggcgagaagc
     4561 gcggtgacct gtactatacc aaggatatgt tgctggccat cggggaccgt tatgaaaatg
     4621 aaattgccgc agcgcgcgcg gcagatgccg cctaccgttg acaagaacgt gttggcgatc
     4681 tctgccggga tgatggtgtc tgatcgatcc gactgatccg atc
//
LOCUS       DASRDH010000247         4138 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1078840,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000247 DASRDH010000000
VERSION     DASRDH010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 4138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4138
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1078840"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..1251)
                     /gene="coxB"
                     /locus_tag="VFA14_05745"
     CDS             complement(91..1251)
                     /gene="coxB"
                     /locus_tag="VFA14_05745"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013236088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HZS80912.1"
                     /translation="MITVKRLRCLLPGASLLAVPMLSWAVVDSQGGPAGWQINFQPPA
                     TQIAEQIHGLHHMMLIICLVIFLAVFGVMFYSILKHRKSLGHRSASFHESTAVEITWT
                     VIPFLIVIGMALPATRTVVAMKDTSNADLTIKVTGMQWKWGYDYLKGEGEGISFLSNL
                     ATPREQITDASLKKNDNYLIEVDNPVVVPVNKKVRLVTTANDVIHSWTIPAFGVKQDA
                     IPGFVRDAWFKAEKIGTYRGQCVELCGKDHAFMPIVVNVVSEADYKTWVDGKKKEMAA
                     KADDPNKVWTIDELKQRGGKVYAANCAVCHQANGKGIGIFPALDGGPSMNGPKLAQIH
                     TVLNGRNAMPAWKSVLSDTEIAAVITYTRNSWSNKAAENIVQPVEVLAARKN"
     gene            complement(1355..1585)
                     /locus_tag="VFA14_05750"
     CDS             complement(1355..1585)
                     /locus_tag="VFA14_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS80913.1"
                     /translation="MNGSKRTLIRNSTAEFCIRNGKAVFDLITFVVAFYTPGDLTRAG
                     AGGLSGLRWSQIVCWLNLYVAGKRGRFSLICL"
     gene            complement(1582..3504)
                     /locus_tag="VFA14_05755"
                     /pseudo
     CDS             complement(1582..3504)
                     /locus_tag="VFA14_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019562028.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme endonuclease
                     domain-containing protein"
     gene            complement(3885..>4138)
                     /locus_tag="VFA14_05760"
     CDS             complement(3885..>4138)
                     /locus_tag="VFA14_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS80914.1"
                     /translation="WNINVLEAAPLPTGWPAMPNTKPFFYLCLIQQLLRTICPHSSWG
                     HRLATLLEKEFPAMPNRAVTLEDLGVCENWGAWDLWRHK"
BASE COUNT          788 a         1247 c         1171 g          932 t
ORIGIN      
        1 catgtacgta atcgtgatcg actacaatcg tactcatttc agcctccagt tcgatgcatg
       61 cgcgcgcctg tccggcgccc ggatttccgt tcagttcttg cgcgcagcca gtacttcaac
      121 cggctggaca atgttttctg ctgctttatt ggaccagcta ttgcgcgtgt aggtgatcac
      181 ggcagcgatt tcggtgtcgc tcagcacgga cttccatgcc ggcatggcgt tcctgccgtt
      241 cagcacagta tgaatctgcg ctagcttcgg accgttcatg gacgggccgc catcgagtgc
      301 aggaaatatg cctatgccct tgccattggc ctgatggcag accgcgcagt tggcagcgta
      361 gacttttccg ccgcgttgct tgagttcatc aatggtccag accttgttcg gatcatccgc
      421 cttggcggcc atttcctttt tcttgccatc gacccaggtt ttgtagtcgg cttcgctgac
      481 gacgttgacg acgataggca tgaaagcgtg gtccttgccg catagttcga cgcactgacc
      541 acggtaggtg ccgatcttct cagccttgaa ccaggcatcg cgcacgaagc caggaatcgc
      601 atcctgtttc acgccgaaag ccgggatggt ccaggagtgg atcacgtcgt tggcggtggt
      661 gaccagacga acctttttgt tgaccggcac gacgacagga ttgtcgactt cgatcaggta
      721 gttgtcgttc ttcttcaatg acgcgtcagt gatctgttca cgcggcgtgg ccagattgga
      781 caggaaggaa atgccttctc cttctccctt gaggtaatcg tagccccact tccattgcat
      841 gccggtgacc ttgatggtca gatcggcgtt ggatgtgtct ttcatcgcga ccacggtcct
      901 ggtggccggc agcgccatgc cgatcacgat caggaaggga atcaccgtcc aggtgatttc
      961 aacggcggtg ctttcatgga aactggccga tcgatggccg agggacttgc ggtgcttgag
     1021 gatggagtaa aacatgacgc cgaacacggc gaggaaaatt accagacaaa tgatcagcat
     1081 catatgatgc aggccatgga tctgttcggc gatctgggtg gccggcggct ggaaattgat
     1141 ctgccatcct gcgggaccgc cctgactatc gaccactgcc catgacagca tcggaaccgc
     1201 caggagcgat gcaccaggca gcaagcaacg aaggcgcttc acagttatca tgatttcctc
     1261 gaaagctcac caatcatcat aggaattacg ccaactccac gaagctactg gtcacggctt
     1321 gtctgccgat gagaccctgc cagttcaatg cgtgtcagag gcagatcaga ctgaagcgcc
     1381 cccgttttcc cgccacgtac aaattcagcc agcaaacgat ctgcgaccag cgcaaaccac
     1441 tcagcccgcc cgcgcccgca cgagttaaat ctcccggagt ataaaatgca accacaaatg
     1501 ttatcaagtc aaaaaccgct ttcccgttcc tgatgcagaa ctcggcggtg ctgttgcgga
     1561 tcagcgtgcg tttgctccca ttcatggcgc atgtccttca gaactggaca ggttccaccc
     1621 ggccgacagc gcctccgcct gctgcaacac cgtctgcaca gccgcatcct gcagatcggg
     1681 cggataaccg tacttgcgca ggatgcgctt gaccagcacg cgcagcaggg ccctggccga
     1741 ctcacggtgc gcccagtcca ccgagacgtt ttcgcgcagg ctgaccagca gttcgtgggc
     1801 gatcacgcga agcttgtcgt caccgatcac ctgcagggcc gattcgttct cggccagtgc
     1861 atcatagaaa gcaatttcct cttgggaaag accctgttct tcacctcggc tgcgtgcggc
     1921 acggatgtcc ttggcaagtt cgatcagttc ttgcagcact tcggcggtgg tgatggtatt
     1981 ggcgtgatag cgtgcaacgg catcttccag acgctcggag aaagcgcggg tttctaccac
     2041 gttggtccgg gtacgggaac ggatactgtc acccagcaat ttgcgcagcg cttccagacc
     2101 caggttcttt tgcttcatct gctggacttc ggccaggaac tcgtccgaca ggatgggaat
     2161 gtccggtgtc tggatgcccg ccacggccag gatgtcgaca atctcggtgg agacgacagc
     2221 gcggctgacg atctgctgga tggccagttc gcgatcctgc cggggagcgc cgacaccgcc
     2281 agcagtcttt gccagcgcaa tacgaatcgc ctggaagaac cccactgctt cacgaatgtc
     2341 cttcgcctcg tccgaactgg cggccagcgc gaaggctttg gacagcgtca atacgccatc
     2401 ggcgaaacgc cgctgcgcct gtttcttgcc ttctgcagtc ttttccgcag cggcccactg
     2461 ttgctgcttg tcgagaatcc attcgatggc ccctgccatc atcgccagtc gatcgctcgg
     2521 agtgccatct aacgcattgc gatagtcgaa accgtggaac atgtcgcgca cgatctcgta
     2581 cttctccagc atcaccgcca cggcctcggc ttcgtcgatc ccggtcttgt cctggtcggg
     2641 cttggagtac cgcgccaatg cggatttcag gttttgtgca ataccgatgt aatcgactat
     2701 cagccctgcc ggcttgtcgc ggaacacgcg gttcacccgc gcgatggcct gcatcagccc
     2761 atgcccatgc atgggcttgt cgatgtacat ggtgtgcatg ctcggtgcat caaagccggt
     2821 cagccacata tcgcgcacga tcaccaacct gagtggattc ttcggatcgc gggcacgctg
     2881 ggctagtaga tcacgatgag ccttattgcc gatatgctgt tgccgctctg gcggatcgga
     2941 agccgcgccg gtcatcacga tctttacgct gccggcgttg tcatcttcgc tgtgccagtc
     3001 cgggcgcagt ttccgaattt cgtcgtacag cgctacacag atgcgccggc tcatgcatac
     3061 caccatggcc ttgccgtcga gcgcggctgc gcgagcctcg aaatgctgca ccaggtcggc
     3121 ggccaccatc gccagtcgcg tcggactgcc cgccagcatt tcgatggtgc tccacttctg
     3181 cttggtgcgc tccgcgctga cctcatcttc accctccagc agggcctcga tctcggcgtc
     3241 gatatgcggc gtttcttcct cgtccagctc gatgcgggcc agccgggatt cataatagat
     3301 cggcaccgtg gcgccgtctt ccaccgcgcg gctgatgtcg tagatgtcga tgtagtggcc
     3361 gaacacagcc ggtgtgttga cgtcggtcgc ctcgatcggc gtaccggtga aaccgatgaa
     3421 ggaggcgttg ggcaatgcat cgcgcaggta cttggcgaac ccgtagccga tctcgccggt
     3481 cttgctgccc accttggcct tgaagcgctg ccggaaagga gtccagccct tgtccttcaa
     3541 gccacggatg tagtccgccg tcgcccgtga tttgaaggcg cccaccagcg cacctgatgc
     3601 ccctgcctgc gaatagtcat agccttccag gcgcaggctg cggcgatggt gggcgttgac
     3661 gatcatgccg taatgtttcc ttgggcatgg gcaggcacaa ggcctgcccc tacggcgccg
     3721 gtcatatgat tgatcgtccc cggctttgga gaggaaggcc atcagcgctg ctttgtgtcg
     3781 ttgccgtttg ataacgatgg atctgtgttt gaccaagaca gaacatctgc tataatcgga
     3841 accaatatga ccggcgcctc tgcactttgt gtacgcgtcg gttctcattt gtgcctccat
     3901 agatcccacg ctccccaatt ttcgcagacg cccagatcct ccagggtcac agcgcgattt
     3961 ggcatagccg ggaattcttt ttccagcagc gtcgccagac ggtgtcccca ggacgaatgc
     4021 ggacagattg ttcgcagcag ttgctgtatc aggcagaggt agaaaaaggg tttggtattg
     4081 ggcatggctg gccaccctgt gggtagcggc gctgcctcca gcacgttgat gttccaca
//
LOCUS       DASRDH010000248         2648 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1428932,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000248 DASRDH010000000
VERSION     DASRDH010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1428932"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..144
                     /locus_tag="VFA14_05765"
     CDS             <1..144
                     /locus_tag="VFA14_05765"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080720.1"
                     /note="catalyzes the formation of
                     1-(5-phosphoribosyl)-5-aminoimidazole from
                     2-(formamido)-N1-(5-phosphoribosyl)acetamidine and ATP in
                     purine biosynthesis; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HZS80915.1"
                     /translation="CGIGMTVIVSRENADAAMAQLKVAGETVFRIGEIRARAVGEAQT
                     IIV"
     gene            252..611
                     /locus_tag="VFA14_05770"
     CDS             252..611
                     /locus_tag="VFA14_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007878301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS80916.1"
                     /translation="MTKNRHKHLLRLSFFLAVACAVHYPAIAAKEDVSRRDPQRWYQG
                     ADTPRLHHENLMKEAHAAYAQALKECKLLRKTEARRACHEEARTNMRSDLARARRIFK
                     AAAAGSRWDGNAAKSGR"
     gene            complement(628..969)
                     /locus_tag="VFA14_05775"
     CDS             complement(628..969)
                     /locus_tag="VFA14_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HZS80917.1"
                     /translation="MIQLNVTIMGHSYTLACKEGEETTLQQAVAYLNEKMRAIRDAGK
                     IKGNDRIAIMAALGIAAELLSTQPGNGFFAGLTIAEWKQNMTAIHTVLDEALEPQENL
                     LQGLSSERATN"
     gene            complement(966..1199)
                     /locus_tag="VFA14_05780"
     CDS             complement(966..1199)
                     /locus_tag="VFA14_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF904 domain-containing protein"
                     /protein_id="HZS80918.1"
                     /translation="MSYELQQLSEKVTQLAALTQSLRRENADLRLDVTTLTTEKTELS
                     RRMEEALQRAQTLLETLPPATPADPTPDEEAAV"
     gene            1275..2114
                     /locus_tag="VFA14_05785"
     CDS             1275..2114
                     /locus_tag="VFA14_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HZS80919.1"
                     /translation="MKEFNEPSSKTTDLCRVARTGIEESSQAAMLVAYLRSITGKNRT
                     LITNRHLAFVLSAIAGAVNAGGFVAVGQFTSHMSGILSQMAEDLAIGAVGLLVAGATS
                     IFFFISGAVYSAMLVNWARKRRMEAEYAMPLIVEAILLLLFALLGGNITRIEWLFVPV
                     TVMLLCFIMGLQNAICTQLSHAEIRTTHVTGMVTDIGIELGKALYWNRNYHSGMRVYT
                     NSAKLKNLMTLVLLFYAGGVAGALGFRHFGFVFTLPLAATLIVIALVPVLDDVRRTLQ
                     QKP"
     gene            complement(2208..>2648)
                     /locus_tag="VFA14_05790"
     CDS             complement(2208..>2648)
                     /locus_tag="VFA14_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006405290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HZS80920.1"
                     /translation="RITYILILFGGAGIPAAIFFHRLYPDRPAQFLLASIITISACLL
                     VLFPCALSIVTLSVHTLIWGSAIVCFGLAMQAWVLRLAPEATDLAVSIYSGLYNVGIG
                     AGALLGNHIAHGVGLPWVGTFGGAIGGIGVGICWLALHQHYSTE"
BASE COUNT          579 a          713 c          763 g          593 t
ORIGIN      
        1 tgtggcatcg gcatgaccgt gatcgtttcc agagaaaacg ccgacgccgc catggcgcaa
       61 ctgaaggtgg caggcgaaac cgtgttccgc atcggtgaaa tccgcgcccg cgccgtagga
      121 gaagcgcaga ctatcatcgt ttgacctgct gcgtggtctt ttgacgccga cgcaaacagg
      181 gtatgataat cggcactgca tcgtgcagac gtaaggacgc tcactgtttc tactgaaagg
      241 agcttgcagc catgacaaaa aatcgccaca aacacctttt gcgactatcg ttctttctgg
      301 ctgtcgcctg cgccgtgcat tatcctgcca tcgccgcaaa agaagacgtc tcgcggcgcg
      361 atccgcaaag atggtaccag ggtgccgata cacccaggct gcatcacgaa aatctgatga
      421 aagaagcgca tgctgcttat gcgcaggcgc tgaaggaatg taaactactc aggaaaacag
      481 aagccaggag ggcctgccat gaagaagcgc gcaccaacat gcgcagcgat ctggcgcgcg
      541 ccagacgtat cttcaaagcc gctgcagcag gcagcagatg ggatgggaac gctgcaaaaa
      601 gcggtcgtta gaaccccgcg cagattctta gttcgtggca cgttctgacg acaatccctg
      661 aagcagattc tcttgcggtt ccagtgcctc atccagcacg gtatgtattg cggtcatgtt
      721 ttgcttccac tccgcgattg tcagtccggc aaagaaaccg ttgccgggtt gtgtcgacag
      781 cagttcggcg gcgatgccga gtgcggccat gatggcgatg cggtcgttgc ctttgatttt
      841 tccggcatcg cggatggcgc gcattttttc attgagatac gccactgctt gctgcagcgt
      901 tgtttcttcg ccttccttgc atgccagcgt ataagaatgg cccatgatcg tgacgttgag
      961 ctgtatcata ccgctgcctc ttcatcgggc gtcggatccg ccggcgttgc gggcggcagt
     1021 gtttccagca gggtctgcgc acgctggagt gcttcctcca tacgccggga tagctctgtc
     1081 ttttcggttg tcagagtggt gacatccagg cgcagatcgg cattttcacg acgcagtgac
     1141 tgggtcaggg cggcaagttg cgtaactttc tctgaaagtt gctggagttc gtagctcatg
     1201 gcctaaaata tatagtgcag ggaatatacg acattatatg ctgttgacag cccggcaaat
     1261 caagaaatac atggatgaag gagttcaatg aaccgtcgtc aaagacgacc gatctttgcc
     1321 gcgtcgcaag aaccggcatc gaagaaagct cacaagccgc catgctagtc gcctacctca
     1381 gaagcatcac cggtaagaac cggacgctca tcaccaaccg gcatcttgcc tttgtgctgt
     1441 cggccattgc cggcgcagtc aatgccggcg ggtttgtggc ggtcgggcaa tttacttcac
     1501 atatgtctgg catcttgtcg cagatggcgg aggatctggc aatcggcgcg gtcggcttgc
     1561 tggtggcggg agcaaccagc atattctttt tcatttccgg cgcggtttac tcggcaatgc
     1621 tggtcaattg ggcgcgcaaa cggcgcatgg aggcggaata cgccatgccg ctgatcgttg
     1681 aagcgatcct gctgctgctc ttcgctttgc ttggcggcaa cattacccgc atcgaatggc
     1741 tgttcgtgcc agtgacggtg atgttgctat gtttcatcat gggtttgcag aacgccatct
     1801 gcacgcaact ctcgcatgcc gagattcgca ctacccatgt cacaggcatg gtcactgaca
     1861 tcggtatcga actgggcaag gcgttgtact ggaatcgcaa ctatcattcc ggcatgcgtg
     1921 tgtacacaaa cagtgccaag ctgaaaaacc tgatgacgct ggtgctgctg ttttatgccg
     1981 gcggcgtggc gggcgcgctc ggttttcggc acttcggttt tgtgtttacc ctgccgctgg
     2041 cggcaacgct gatcgtcatt gcgctggtgc cggtgctgga cgatgtgcgc cggaccttgc
     2101 aacagaagcc atagtacctc tcatcgggaa ggaatattga ctatcatcag catcatggcg
     2161 ctggcatcat actgggacat tggagttcgc gccggacgga ctcgcgttca ttcggtgctg
     2221 taatgctggt gcaatgccag ccagcaaatg cctaccccga ttcccccaat tgcgccaccg
     2281 aatgtaccga cccacggcaa tccgactccg tgcgcaatat gattgccgag gagagctcct
     2341 gcgccgattc ctacgttata taggccagaa tagattgaca ctgcgagatc tgtcgcttct
     2401 ggcgccaatc tcagcaccca tgcttgcatc gcgaggccga agcaaacgat tgcactgccc
     2461 cagatcaagg tgtgaacgga cagtgtgacg atgctgagag cacagggaaa caggaccagc
     2521 aggcatgcag atattgtgat gatcgaggcc agcaggaatt gcgctggtcg atcagggtag
     2581 aggcgatgga agaaaatggc cgcgggaatc ccggcgccac caaacagtat caggatgtag
     2641 gtgatccg
//
LOCUS       DASRDH010000249         2482 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_915532,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000249 DASRDH010000000
VERSION     DASRDH010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2482
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_915532"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..863)
                     /locus_tag="VFA14_05795"
     CDS             complement(<1..863)
                     /locus_tag="VFA14_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008116968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HZS80921.1"
                     /translation="MTTPQFVHLRLHSEYSIVDGLVRIDELVKAAAKDDQAALAITDL
                     GNLFGLVKFYKGARGKGIKPIAGCDVWVSNDTDRDKPSRLLLLVKDHHGYLQLCDLLS
                     RAWLTNLHRGRAELRLEWLEQLRHDEGGNGLIALSGAHLGDIGMAIGNANVAAAECCA
                     RRWAEIFPGAFYVEIQRIDQPGMEVQVRQSVALAARLGLPVVATHPIQFLSQEEFVAH
                     EARTCIAEGEMLSSARRARRFNEQQNFKSQAEMTVLFADLPGALQNTIEIARRCNLTL
                     TLGKPQLPDFPT"
     gene            931..1824
                     /locus_tag="VFA14_05800"
     CDS             931..1824
                     /locus_tag="VFA14_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HZS80922.1"
                     /translation="MQETRNSYVNITAYKFITLDDTVEKRPVFLTFCQRHDLCGTIIL
                     SPEGINLFLAGRRADIDAFLTWLRADARFTDIEVKESHSGQQPFNRMLVKLKAEIITM
                     KHPLIKPEEGRAPCVAAKTLKRWLDQGHDDNGKPVVMVDTRNAFEVDVGTFENTIDYR
                     ISKFTEFPQVIANHKDQLKDKTVVTFCTGGIRCEKAAIHMQNIGYDSVYQLEGGILKY
                     FEEIGGAHYQGDCFVFDYRTALNPQLRETLTTQCFACRAVVTPREQLSAAYVPGESCP
                     HCAPEPAASANTAPALRHQLT"
     gene            complement(1933..>2482)
                     /locus_tag="VFA14_05805"
     CDS             complement(1933..>2482)
                     /locus_tag="VFA14_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HZS80923.1"
                     /translation="FAPGDEGLLKGSGRSIAGFHLRDALDLVSKHAPSVITRFGGHAM
                     AAGLTIRAEAFDAFSGAFEKIGKDWLSQNQLERVIETDGVLEDACYSISFVSLLDQQV
                     WGQGFAPPVFCDRFRILRQRVLKEKHLKLLLEKEGRQYDAIWFSHADALPDYATVAFK
                     LDANEYNGTTTVQLMVEHAEAA"
BASE COUNT          558 a          779 c          668 g          477 t
ORIGIN      
        1 gtcgggaagt ccggcaactg cggcttgccc agcgtcagtg tcaggttgca gcgcctggca
       61 atttcgatag tattttgcaa cgcgcctggc aaatcggcga acagcaccgt catctctgcc
      121 tgagacttga aattctgctg ctcattgaag cggcgcgcgc gccgcgcgct ggacagcatc
      181 tcaccttcgg cgatacaggt acgcgcctca tgggcaacga attcctcctg tgacaaaaac
      241 tgtatcggat gcgtcgccac caccggtaaa ccgagacgcg ccgccagagc gaccgactga
      301 cgcacctgca cctccatgcc gggctgatcg atgcgctgga tttcgacata aaaagcgccg
      361 ggaaaaatct cggcccaacg ccgggcacag cactcggccg ccgccacgtt agcgttgccg
      421 atagccatgc cgatgtcacc gagatgcgcg ccagacaaag cgatcaggcc gttaccgccc
      481 tcatcatggc gcaactgttc gagccactcc aggcgcagct ccgcgcggcc gcgatgcagg
      541 ttggtcagcc aggcccgact caacagatcg cataactgca aatagccgtg atggtccttg
      601 accagcagca gcaggcgcga tggcttgtcg cggtcagtat cgttgctgac ccagacgtca
      661 caaccggcaa tcggcttgat gcccttgccg cgcgcacctt tgtaaaactt gaccaggccg
      721 aacagattgc ccagatcagt gatcgccagc gcagcctgat cgtcttttgc cgccgcctta
      781 acaagctcgt cgatgcggac caggccatcg acgatggaat attcggaatg aagccggaga
      841 tgaacgaact gcggtgtagt catggggacg gtattctaca cgtgccgttt gttataatgc
      901 tggcagttca aaaacccggg tagcagcgcc atgcaggaaa ccaggaactc ttacgtcaat
      961 attacggctt acaagttcat caccctcgat gacacggtgg aaaaacgtcc tgtcttcctg
     1021 acattctgcc aacggcacga cctgtgcggc accatcattc tgagcccgga aggcatcaac
     1081 ctgtttctcg ccggccggcg ggccgacatc gatgcctttt tgacatggct gcgcgccgac
     1141 gcccgtttta ccgatatcga agtcaaggaa agccattccg gacagcagcc tttcaaccgc
     1201 atgctggtca agctgaaagc cgaaatcatc acgatgaagc atccgttgat caagccggaa
     1261 gaaggccgcg cgccttgcgt cgcagccaaa acgctcaagc gctggctcga ccagggccac
     1321 gacgacaatg gcaagccagt ggtcatggtc gatacgcgca acgcctttga agtcgatgtc
     1381 ggcacgttcg agaacaccat cgattaccgc atcagcaagt tcaccgaatt cccgcaagtc
     1441 atcgccaacc acaaggacca actcaaagac aagacggtcg tcactttctg caccggcggc
     1501 atccgttgcg aaaaagccgc gatccacatg cagaacatcg gctacgacag cgtgtatcag
     1561 ctcgaaggcg gcattctcaa atatttcgaa gaaatcggcg gcgcgcatta tcagggcgac
     1621 tgcttcgtct tcgactaccg caccgcgctc aacccgcagc tgcgggaaac cctgacgaca
     1681 caatgctttg cctgccgcgc cgtggtgacg ccgcgcgaac aactgtcggc ggcgtatgtg
     1741 ccgggcgaat cttgcccgca ttgtgcgccg gaaccggcgg caagcgccaa tactgcgcca
     1801 gctttgcgcc atcagttgac ctgactgtcc cggctatcgc gcagggctcc cctgacgtga
     1861 tctgccaaag cgcgctccgg caaaaccgct cgttccagga acgtggttac gatccaagaa
     1921 gctgctcaga atctaagccg cttccgcatg ctccaccatc aactgtaccg tggtcgttcc
     1981 gttatattcg ttggcatcca gtttgaaggc aaccgtcgcg taatccggca aggcgtcggc
     2041 gtggctgaac cagatggcat cgtattgtcg cccctctttc tccagcaaca gtttgagatg
     2101 cttttctttc agaacgcgct gcctaagtat gcggaaacgg tcgcaaaaca ccggcggcgc
     2161 gaaaccctgt ccccacacct gttgatccag caggctgacg aaactgattg agtaacaggc
     2221 gtcttcgagc acgccgtcgg tttcgatcac ccgttccaac tggttctggc tcaaccagtc
     2281 cttaccgatt ttttcaaatg cgcctgaaaa cgcatcgaag gcttcggcgc gtatcgtcag
     2341 gccggctgcc atggcgtgac cgccgaatct ggttatcacc gaaggcgcat gcttggatac
     2401 cagatccagc gcatcgcgca ggtgaaagcc ggcaatcgaa cgaccggacc ccttgagcag
     2461 accctcgtcg cccggcgcga aa
//
LOCUS       DASRDH010000250         2009 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_680264,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000250 DASRDH010000000
VERSION     DASRDH010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_680264"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="VFA14_05810"
     CDS             <1..180
                     /locus_tag="VFA14_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HZS80924.1"
                     /translation="SREKLMEMARGREYEVFDRSLDVQVSRLRKLIEPDPANPLYIQT
                     VWGLGYVFIPEGKPR"
     gene            212..1579
                     /locus_tag="VFA14_05815"
     CDS             212..1579
                     /locus_tag="VFA14_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZS80925.1"
                     /translation="MNDQTRQRWFGNGLFWRTFILLSFLITASMVAAWVASFHMAERG
                     PRTEQLAAQIVSMATITRAALTHSAPEMRRELLLELASNEGIRIYPLEKSDKIAPPGN
                     SPALPGLEYYVKQSMGDNTRFSSRVNGIDGFWISFNIDEDAYWLMLDRGRLNHTSSLQ
                     WLGWASIAMALSLIGAVFISTLINQPLVRLTAATRAIAKGQQPDLLPESGPTEIREAN
                     RSFNQMVNDLNRIESDRAVILAGISHDLRTPLARMQLEVEMAQLPDEARDGMHSDLAQ
                     MDAIIGQFLDYAKPFDSSSFIPVDLSSLLEDAANHAKRLNDVCVNTAIMPGISIMGNT
                     VELKRLISNLTENARRYGKTPGTDGTDIDILCKPDGHRIKVEISDHGVGAPESELEYL
                     LRPFTRLDTARGQANSSGLGLAIVNRIVIRHNGRLHLRNREGGGLIVQMIFPRHHGHP
                     GRHAG"
     gene            complement(1649..>2009)
                     /locus_tag="VFA14_05820"
     CDS             complement(1649..>2009)
                     /locus_tag="VFA14_05820"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HZS80926.1"
                     /translation="VLHRHAGIAAFDQDVFINAVGGVRITEPAADLAVLLAIDSSMRD
                     RPLPRGLVVFGEVGLAGEIRPAPRGQERLREAAKLGFSMAMIPKANAPRQEIEGLKAV
                     AVERIDEALQKIRDIGE"
BASE COUNT          438 a          625 c          550 g          396 t
ORIGIN      
        1 tcgcgtgaaa aactgatgga aatggcacgc gggcgcgaat atgaagtttt cgaccgcagt
       61 ctcgatgtgc aggtttcacg tttgcgcaaa ctgatcgagc ctgatccggc caatcccttg
      121 tatattcaaa cggtctgggg cctgggctat gttttcattc ccgaaggcaa accgcgctga
      181 acgcgcaaca ctgttcctgc aacaccacca catgaacgat caaacgcgcc aacgatggtt
      241 cggcaatgga ctgttctggc gtactttcat cctgttgagc tttctgatta ccgccagcat
      301 ggtagccgca tgggtggcca gtttccacat ggcagaacgt ggcccgcgca ctgaacagct
      361 ggcggcacag atcgtttcca tggctaccat cacacgcgct gcgttgacgc actccgcacc
      421 ggaaatgcgg cgcgagctgc tgctcgaact tgccagcaac gaaggcattc gtatttaccc
      481 gctggaaaaa tcagacaaaa tcgcgccacc cggcaactct cctgctttgc ccgggctgga
      541 atactacgtc aagcaatcga tgggcgacaa cacccggttt tcctccagag tcaacggcat
      601 cgatggattc tggatcagct tcaacatcga cgaagacgca tactggctga tgctcgatcg
      661 cggccgtctg aatcacacct ccagcctgca atggctgggc tgggcatcga ttgcgatggc
      721 gctctcgctg atcggcgcgg tgttcatctc gacgttgatc aatcagccgc tggtgcgcct
      781 gacggccgcc acgcgcgcga tcgccaaagg tcagcagccc gatctgctgc cggagtccgg
      841 cccgaccgaa atcagggaag ccaaccgcag cttcaaccag atggtgaatg atctcaatcg
      901 tatcgaatcc gatcgcgccg tcatccttgc cggtatttca catgatttgc gcacgccgct
      961 ggcgcgtatg cagcttgaag tcgaaatggc ccagttgccg gatgaagcgc gcgacggcat
     1021 gcattccgat ctggcgcaaa tggatgccat catcggccag ttcctcgatt atgccaaacc
     1081 tttcgatagc agcagtttca ttccggtcga tttgtccagc ctgctggaag atgccgccaa
     1141 tcatgccaaa cgcctgaacg atgtgtgcgt gaacacggca atcatgccgg gcatcagcat
     1201 catgggcaac accgtcgaac tcaagcgcct catcagcaac ctgaccgaga acgcccgccg
     1261 ctatggcaaa acgccgggta ccgacggtac tgacatcgat atcctgtgca aacctgatgg
     1321 ccaccgcatc aaggtcgaaa tcagcgatca tggcgtcggc gcgccagaga gtgaactgga
     1381 gtatttactg cgccccttca cccgcctcga taccgcgcgc ggccaggcca acagttccgg
     1441 cctgggcctg gccatcgtca accggatcgt gattcgtcac aacggcagat tacatctgcg
     1501 caatcgcgaa ggcggcgggc tgatcgtaca gatgatcttc ccccggcatc atggtcatcc
     1561 gggcagacat gcaggctgag tcagcagcga cgtccgtgac catgctgctg gtggcggcgt
     1621 taaccagacc tgaatgcttc gggagggact attcgccgat atcgcggatc ttctgcaagg
     1681 cctcgtcgat acgctcgacc gctaccgcct tcagaccttc gatctcctgc ctgggcgcgt
     1741 tcgccttggg aatcatagcc atcgaaaacc ccagtttggc ggcttcgcgc agacgctcct
     1801 gaccgcgcgg cgctggacgg atctcaccgg ccagaccgac ttcgccaaat accacgaggc
     1861 cgcgcggcag cggtctgtca cgcatggatg aatcgatcgc cagtaacacc gcaagatcgg
     1921 cagccggctc agtgatcctg acaccaccga cagcattgat gaagacatcc tgatcgaacg
     1981 ccgcgatgcc ggcgtgacga tgcagcact
//
LOCUS       DASRDH010000251         5011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_518474,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000251 DASRDH010000000
VERSION     DASRDH010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 5011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5011
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518474"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="VFA14_05825"
     CDS             <1..372
                     /locus_tag="VFA14_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017330508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HZS80927.1"
                     /translation="PIAAGLGAYFELQHEEMYGERFDIPQPDTTVFISWFEGGEVFRS
                     GCCWERGHGRIFYFRPGHEAYPTYHDSKVQRVIANAVQWAAPRVNIVDCCPMSPPLEK
                     LSEKSGQFAHVGIQQSQGDIT"
     gene            369..1502
                     /locus_tag="VFA14_05830"
     CDS             369..1502
                     /locus_tag="VFA14_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017330507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZS80928.1"
                     /translation="MNDDYKVELPEVAAIARGRRLRVGVIGLGIGRKHIEGWREHPDV
                     DVVAIADLDGKRLDRIGEQFGIGARYATMDEMLATERLDVVSVCTPNKFHHDLTIAAF
                     GAGCHVLCEKPMAMNAEEGRKMLAAAQRASRRLMINFSYRFSAQSRALKAQVDAGVFG
                     DFYFGRTVWHRRRGMPGFGGWFGTKALAGGGPLIDLGVHRLDLALWLMGYPKPVWVMG
                     SAYDPIAGELAARSGKTFDVEDLAAAMIRFDNGATLMIEASWAANIQEAELMETRLLG
                     TRAGLLQKNLNEGYTFDAHIFMEQNGAQFDMRLNPPADGAHSAMYDYAEAILCDAPHP
                     APGEEGLIVMEILDAIYASAAAGAPICVHASGQDVVAPIMQSG"
     gene            complement(1504..2703)
                     /locus_tag="VFA14_05835"
     CDS             complement(1504..2703)
                     /locus_tag="VFA14_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis protein HemY"
                     /protein_id="HZS80929.1"
                     /translation="MRILLWLLSLSVLAVGLALLARFNPGNVVLFYPPYRVDLSLNFF
                     LFIIVLLLIALHLLIRAVRMMQELPGRVIAYRRQKHENDGNKALRDSLKAFFEGRFGH
                     AEKAAMRAAELPENTGIAALIGARAAHRMRQTGRRDNWLANIDSDAGLKAARLMTTLE
                     LQVDDHQFMAALKTVDELNANGTRHIQALQWALKANQQAKNWPEVLRLTQTLDKRNAL
                     HPALSNRLREMAYDALLSDSAHDAESIRRLWADVPQADRLKPFIAARAAHAFSSRGLH
                     DEARTLLERALAAAWDIRLIRAYRDSTADTGSPALLTQIERCESWLTRRPTDAELELT
                     LGMFCLRQKLWGKAQRHLERALADAIEPRTVRESHLSLAQLHDALDQHQQANAHYRQC
                     ALATAIR"
     gene            complement(2722..3891)
                     /locus_tag="VFA14_05840"
     CDS             complement(2722..3891)
                     /locus_tag="VFA14_05840"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007881389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HZS80930.1"
                     /translation="MNELPSSPGRNQPGTQADTGSSAGIALPSSIPPNDGILKKRLRR
                     LTLLIIALAVALVGQWWISHTEIRSLRAEVARRLQTGDVADTEIKGTLKAIQESAREL
                     QARITVLDNKQTESQGQQLALEQLYQDLSKNRDDWALSEIEEVLSTASQQLELAGNVQ
                     GALIALQNADRNLSRSDKPQFITIRRAIARDRDRLQALPTVDIPGIAVRLDNIIGQID
                     GMPLLSDEKPVMSTAPRKQPAHSTPKKAGAHAGSNGKGQATSGWLTNAQNTWQNLNAE
                     MWTELKQLVRIRSVDTPDALLLSPTQAYYIRENLKLRLLNARLALLSRNEFAFRNDLR
                     AAQGAIAKYFDTRVRQVQTTQASLKQIQGNNLSIQMPTLSESINAVRNYKSRRSN"
     gene            complement(3888..4670)
                     /locus_tag="VFA14_05845"
     CDS             complement(3888..4670)
                     /locus_tag="VFA14_05845"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007881388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HZS80931.1"
                     /translation="MKERGFRPVVVTRPLAQAGAFAARVADIGRAPVIFPLLDIAPVA
                     DSAALQGMLSVLDRYALAVFVSPNAIDAVFPFIPAWPREVAIGIVGEGSRLALQRHGI
                     DDGNTTIYAPRHRERMDSEELFKVLNLERLHGRRVLIVRAQNGRELLTDMLRAKRIEV
                     DHVTAYQRLLPCIDAARAEQLRRLIDADSDWIITSSEALRNLLNITAETLGSDYVVKL
                     QRNAIIVSHQRIAETAQSLAFSHVTMAGSGDEQLIAALQSQP"
     gene            complement(4718..>5011)
                     /locus_tag="VFA14_05850"
     CDS             complement(4718..>5011)
                     /locus_tag="VFA14_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HZS80932.1"
                     /translation="PLNHSESALTAAAERAVSCAFGGNCQIPLAAFATLDGEHMHMRA
                     MVATPDGQQIAAAQASGPADAPDVLGKQLADLLRAQGAEAILAACRADAAGPE"
BASE COUNT          936 a         1466 c         1569 g         1040 t
ORIGIN      
        1 ccgatcgcgg ccgggcttgg cgcatacttc gaactgcagc acgaggagat gtatggcgaa
       61 cgcttcgaca ttccgcaacc ggatacgaca gtgttcattt cgtggttcga aggcggcgag
      121 gtattccgct ccggttgctg ctgggagcgc ggccacggac ggattttcta tttccgtccc
      181 gggcacgagg cttatccgac ctatcatgac agtaaggtcc agcgtgtgat cgcaaatgcg
      241 gtgcagtggg ccgcgccgcg cgtcaacatc gtggattgct gtccgatgtc gccgccgctc
      301 gaaaaactga gcgagaagtc cggacagttt gcgcacgtcg gcattcagca atcccaggga
      361 gacatcacgt gaacgacgat tacaaggtcg agttgcccga agtggcggca attgctcgtg
      421 gtcgccgtct gcgcgtcggc gtgatcgggc ttggcatcgg acgcaaacat atcgagggct
      481 ggcgcgagca tccggatgtc gatgtcgtcg cgatcgctga tctcgacgga aagcggctcg
      541 acaggatcgg cgagcagttc ggcatcggtg cgcgctatgc gaccatggac gagatgctgg
      601 ccaccgagcg cctggatgtc gtcagtgtct gcacaccgaa caaatttcac cacgacctga
      661 cgatcgccgc gttcggcgcc ggttgccacg tgctgtgcga gaagccgatg gcgatgaatg
      721 ccgaagaggg gcgtaaaatg ctggctgccg cgcaacgcgc cagtaggcgg ctgatgatca
      781 acttttcgta tcgtttcagc gcgcagtcgc gtgcgctgaa agcgcaggtc gatgccggcg
      841 tcttcggcga cttctatttc ggtcgaacag tctggcatcg tcgacgcggc atgccgggtt
      901 tcggcggctg gtttggcaca aaggcactcg cgggcggtgg cccgctgatc gatcttggcg
      961 tgcatcgtct cgatctcgca ttatggctga tgggctatcc gaagccagtg tgggtgatgg
     1021 gcagcgccta cgatccgatc gccggcgaac tcgctgcacg ttccggcaag accttcgacg
     1081 tcgaggatct tgcggctgcg atgatccgct tcgacaatgg cgcgacgctg atgatcgagg
     1141 catcgtgggc cgcaaacatt caggaagcgg aactgatgga aacacgtctg ctcggcacac
     1201 gcgcagggct gctgcagaag aatctgaacg aaggttatac gttcgacgcg cacatcttca
     1261 tggagcagaa cggcgcgcag ttcgacatgc gcctgaatcc gccagcggat ggcgcgcatt
     1321 cggcgatgta cgactatgcg gaagcgattc tctgcgacgc gccgcatccg gcgccgggtg
     1381 aggaaggcct gatcgtgatg gagattctcg acgcgatcta cgccagcgca gcggccggcg
     1441 cgccgatctg cgttcacgct tctgggcagg atgttgtcgc gccaatcatg caaagtggct
     1501 gatctagcga atcgccgtcg ccaatgcgca ctgcctgtaa tgcgcattgg cctgctgatg
     1561 ctgatcgagc gcgtcatgca gttgcgccag gctcaggtgt gactcgcgca cggtgcgtgg
     1621 ctcgatagca tcggccaggg cacgctccag atggcgctgg gccttgcccc acagcttttg
     1681 gcgcaggcag aacataccca gcgtcagctc cagttcagcg tcggtcgggc gtcgcgtcaa
     1741 ccaggattca cagcgttcga tctgtgtcag caacgctggc gaacccgtgt ccgccgtcga
     1801 atcgcggtag gcgcggatca ggcgaatatc ccaggccgcc gccagcgcac gttccagcaa
     1861 ggtgcgcgcc tcgtcgtgca aaccacggct gctgaaagca tgcgccgcgc gcgcggcgat
     1921 gaaaggtttg aggcgatcag cttgcggcac gtcagcccac agacggcgga tcgattcggc
     1981 atcatgcgcg ctatccgaca gtaaggcgtc ataggccatt tcgcgcaggc ggttggataa
     2041 ggccgggtgt agcgcgttac gcttgtccag tgtctgcgtc aaacgcagca cttccggcca
     2101 gtttttggct tgctgattgg ctttgagcgc ccattgcagt gcctggatgt ggcgtgtgcc
     2161 attggcgttg agttcgtcga cggttttcag cgcagccatg aactggtgat cgtcgacctg
     2221 caactccagc gtcgtcatca ggcgtgccgc cttcaggccg gcatcgctgt caatgttggc
     2281 cagccagttg tcgcgacgcc cggtctggcg catgcggtgc gcggcgcgcg cgccgatcag
     2341 cgctgcgatg ccggtgtttt ccggcagttc ggcagcgcgc atggcggctt tttcggcgtg
     2401 accgaaacgg ccttcgaaaa aagccttcag ggaatcgcgc agcgccttgt tgccatcatt
     2461 ctcatgtttc tggcgacgat aggcgatgac gcggccgggc agttcctgca tcatacgcac
     2521 agcgcgaatc agtaggtgca gtgcgatgag caacagtaca atgatgaaca ggaaaaaatt
     2581 cagcgacaaa tcgacccggt aaggcggata gaacaacacc acgttgccgg gattgaagcg
     2641 cgccagtagc gccaggccaa cggctaggac agacagggag agaagccaga gaagaattcg
     2701 catagggact gtgccgccgc tctaattgct gcgccggctt ttgtaattgc gcacggcgtt
     2761 gatgctttcg ctgagcgtcg gcatctggat cgacagattg ttgccctgga tctgtttcaa
     2821 cgatgcttgt gtggtttgca cttgcctgac gcgggtgtcg aagtatttgg cgatggcacc
     2881 ctgcgccgcg cgcagatcat tacggaatgc gaactcatta cgtgacagta gcgccagacg
     2941 tgcattgagc aggcgcagct tgagattttc gcggatgtag taagcctggg tcggcgatag
     3001 cagcagcgcg tcgggtgtat cgacactgcg aatacgcacc agttgcttca attcggtcca
     3061 catttctgcg ttcaggtttt gccatgtgtt ctgcgcattg gtcagccagc cgctggtcgc
     3121 ctgtcctttg ccgttcgacc cggcatgtgc gccggctttc ttcggcgtgg agtgcgccgg
     3181 ctgtttcctg ggagccgtgg acatcacagg cttttcatcg gatagcaaag gcatgccatc
     3241 gatctggccg atgatgttgt ccagccgtac tgcgatgccg ggtatgtcaa cggtcggtaa
     3301 agcttgcaga cgatcccggt cacgcgcgat ggcgcggcgg atggtgatga actgcggctt
     3361 gtcggagcgc gacaggttcc tgtcggcatt ttgcaaagcg atcagcgcgc cctgcacgtt
     3421 gccagccagt tccagttgtt ggctggccgt cgacaggact tcttcgattt cagacagcgc
     3481 ccagtcgtca cgattcttcg agagatcctg atacaactgt tccagcgcca gttgctggcc
     3541 ttgcgactcg gtctgcttgt tatcgagtac ggtgatcctg gcttgcagtt ccctagcgct
     3601 ctcctgtatt gccttgagcg tgcccttgat ttcggtgtcg gcgacatccc cggtttgcag
     3661 cctgcgcgcg acttctgcgc gcaggctgcg gatttcggta tgtgatatcc accactgtcc
     3721 taccagtgca acggccagcg cgatgatcaa caatgtcagc cgccgcagac gttttttcaa
     3781 tatgccgtcg ttcggtggaa tggaggaggg gagtgcaatg cccgccgacg atccggtatc
     3841 cgcttgcgtg cccggctgat tcctgcctgg ggaagatggc aattcattca tggctgggat
     3901 tgtaacgcgg caattaattg ttcgtcgcct gatccagcca tggtcacatg ggagaaagcc
     3961 agtgattgcg ccgtttctgc gatcctctgg tgtgaaacga tgatggcgtt acgttgcagt
     4021 tttaccacat agtcgctgcc gagcgtctct gccgtgatgt tcagcagatt gcgcagggcc
     4081 tcggaactgg tgatgatcca atcgctgtcg gcatcgatca gtctgcgcaa ttgttccgcc
     4141 ctggccgcat cgatgcacgg cagcagacgt tgataggccg tcacatgatc gacttcgatc
     4201 cgtttggcgc gcagcatgtc agtcagcagt tcgcggccgt tctgggcgcg cacgatcagc
     4261 acgcgcctgc catgcagtcg ctccagattg agcaccttga acaactcctc ggaatccatg
     4321 cgctcgcgat ggcgcggcgc gtagatggta gtgttaccgt catcgatgcc atgccgctgc
     4381 aacgccagcc ggctgccttc gccgacgatg ccgatggcca cttcccgcgg ccatgccggg
     4441 atgaaaggga aaacggcatc gatggcattc ggactgacga acaccgccag cgcgtaacga
     4501 tccagcacag ataacatccc ctgcaatgcg gcgctgtcag ccaccggcgc aatgtccagc
     4561 agtgggaaaa tcactggcgc gcggccaatg tcagccaccc gcgcggcaaa cgcaccagcc
     4621 tgcgccaagg ggcgcgtaac gactaccggc ctgaaaccgc gttccttcac agtactccct
     4681 cccgagccag agaaataatg aagaatatga tccagtccta ttccggcccg gcagcgtcag
     4741 cccgacatgc cgccagaatc gcttcagcgc cctgcgcccg cagcaggtcg gcaagctgtt
     4801 tgcccaggac atctggtgca tcggccgggc cgctggcctg tgccgctgca atctgctgac
     4861 cgtcaggcgt cgccaccatg gcgcgcatgt gcatatgctc accgtcgagc gtggcaaatg
     4921 ccgctagcgg gatctggcaa ttgccgccaa aagcgcacga caccgcgcgc tccgccgccg
     4981 cggtcagcgc gctctcacta tggttcagcg g
//
LOCUS       DASRDH010000252         2551 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_574891,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000252 DASRDH010000000
VERSION     DASRDH010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_574891"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2331)
                     /locus_tag="VFA14_05855"
     CDS             complement(<1..2331)
                     /locus_tag="VFA14_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005665063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHYT domain-containing protein"
                     /protein_id="HZS80933.1"
                     /translation="MTSTGLLELFSPSRDPALLHYGTYTPSLVLLSVLVAIFSSWMSL
                     QMADQARASSNQWLRIATLSGASLALGYGVWSMHFIGMLAFNLCTNVEYDPVLTLLSV
                     LPSLASSSVVLTIISRQDASPWSLLGGGMLVGSGIGAMHYSGMAAMQLPLDQRYDPWM
                     FSLSIVVAIVLATLALWVRFGLRKADGLSKRMKVTISATTMGCAIAGMHYIGMEAVHF
                     IGHLPPGNTPGQSSTFLALTLSLTTLILTISVLLANGLLRYHELFLQLSKSESWMRAL
                     LTTTIDGVITVDTDGTILEYNASAERIFGWSRAAIIGRNIRLLMADPERPEQGSAFDY
                     LRTAITDANSEMMAVRQDGTLVPIRCAIGHARQAKDELFVLFITDISERRTIMQALRD
                     SEQQLRSLIGNIPGISFRTSAEAGFPLVFISDGIERVAGYAAADFLPPQACRTLADLI
                     IPSDYSRVAAGLTQAVKQASSYQIEYRLHHADGSLHWMWENGAAMRDEDSSTVRWIDG
                     VILDISERRQMEEELREAKEKAEQAAAARSIFVANMSHEIRTPMNAILGFTDVLLDTP
                     LNADQRRYLDTVRSQSRALLRLLNEILDKAKLDKGAVELELADYNLLALVEELSSTYG
                     NNARSKGLALDTLYDPQLPAWLHGDELRMRQVLCNLLDNAIKFSSVGQINLEVSKNDQ
                     QLHIKVKDTGIGIAPERLEAIFDPFTQADASMTRRYGGTGLGTTISKQLVELMGGRIW
                     ATSGQGIGTTFYITLPLVAAHGSPEQPQHGSLEQSHQ"
BASE COUNT          514 a          711 c          790 g          536 t
ORIGIN      
        1 ctggtgcgac tgctccaggc tgccatgctg cggctgctcc ggactgccgt gcgcggccac
       61 cagcggtagt gtgatgtaga aggtcgtacc gataccttgt ccgctggtgg cccagatacg
      121 ccctcccatc agctccacca actgcttgct gatggtggta cccaggccag tgccgccgta
      181 gcgtcgtgtc atggaggcgt cagcctgcgt gaaaggatcg aaaatcgctt ccagccgttc
      241 gggcgcgatg ccgatgccgg tgtccttcac cttgatgtgc aattgctgat cgtttttgct
      301 gacttccagg ttgatctgac caacggagga gaacttgatg gcattgtcga gcagattgca
      361 gagtacctgt cgcatacgca attcgtcacc gtgcagccag gccggcaatt gtgggtcgta
      421 gagggtgtcg agcgccagac ccttgctacg ggcgttgttg ccgtaggtgg atgacagttc
      481 ctcgaccaaa gccagcaggt tgtaatcggc cagttccagt tctacggcgc ccttgtccag
      541 cttggccttg tcgaggattt cgttgagcag gcggagcagt gcgcggcttt ggctgcgcac
      601 cgtgtcgagg taacggcgct ggtcggcgtt tagtggggta tcgagcagca cgtcggtgaa
      661 gccgaggatg gcgttcatcg gcgtgcggat ttcatggctc atattcgcca cgaagatgga
      721 acgcgcggca gcggcctgtt cagccttttc cttggcctca cgcagctctt cttccatctg
      781 ccgccgttcg ctgatgtcga ggatgacgcc atcgatccag cgcactgtgc tgctgtcttc
      841 atcgcgcatg gcagcgccgt tttcccacat ccagtgcagg ctgccgtcgg catggtgtag
      901 gcggtattcg atctggtaag aactggcttg ttttacggct tgcgtgagtc cggccgcaac
      961 tcgcgagtaa tcggacggga tgatcaggtc ggccagcgtg cggcacgcct gcggcggcag
     1021 aaaatcggca gcagcgtagc cggccacccg ttcgatgccg tcactgatga agaccagcgg
     1081 aaagccggct tcggcgctgg tgcgaaagga gataccgggg atattgccga tcagcgaacg
     1141 gagttgttgc tcgctgtccc gcagcgcctg catgatggtg cgccgttcgc tgatgtcggt
     1201 aatgaacagc acgaacagtt cgtctttagc ctgacgcgcg tggcctatgg cgcaccgtat
     1261 cggtaccagc gtgccgtctt ggcgcaccgc catcatctcg ctgtttgcat cggtgatggc
     1321 ggtgcgcagg taatcaaacg cgctgccctg ttccgggcgc tctggatcgg ccatcaacag
     1381 ccggatattg cggccgatga tagcagcacg cgaccaaccg aagatacgtt cagccgaggc
     1441 gttatattca agaatagtgc cgtcggtgtc gaccgtgata acgccgtcaa tggtcgtggt
     1501 cagcagcgca cgcatccagc tctcgctttt gctcagttgc aaaaacagct cgtggtagcg
     1561 cagcagacca ttggccaaca acactgagat ggtgaggatg agggtagtca gcgacagcgt
     1621 caacgccagg aaagtgctgc tctggccggg cgtgttgcca ggcggcagat ggccgatgaa
     1681 atgtaccgcc tccatgccga tgtagtgcat gccggcaatg gcgcagccca tggtggtggc
     1741 gctgatggtt accttcatgc gtttgctcag gccatcggct ttgcgcagac cgaagcgcac
     1801 ccatagcgcc agtgtggcca gcactattgc caccacgatc gacaggctga acatccaggg
     1861 atcgtagcgc tggtcgagtg gcagctgcat tgcagccatg cctgaataat gcatggcgcc
     1921 gatgccacta ccgaccagca taccaccgcc cagcagtgac caggggctgg catcttggcg
     1981 gctgatgatg gtcagcacga cgctggagga ggccaggctg ggtaagacgg ataagagagt
     2041 taagacggga tcgtactcga cgttggtaca caggttgaag gccagcatgc cgatgaagtg
     2101 catcgaccac acgccgtagc ccagcgccag actggcgccg ctgagggtgg cgatgcgcag
     2161 ccattgatta ctgctggctc tggcctggtc tgccatctgc agactcatcc aggaagaaaa
     2221 gatcgccacc agcaccgaca gcagcaccag tgacggcgtg taagtgccgt aatggagcag
     2281 ggctggatcg cgggaggggg aaaataattc gagcaatcca gtcgatgtca tcacactatc
     2341 cttaagcggg aaatggcgct ggactgcgcc gcttcattgc actatcacat ctcagtcatg
     2401 aatgatacta cctgttcagg cgtctacgta tgacatgaaa aagcctgcat gctgcaggct
     2461 ttttcaggtg taaaaagaat ttactcaaga gaaattcctg gcagcgaaac tccagttcac
     2521 gataccccag aatgcttcga cgtacttggc g
//
LOCUS       DASRDH010000253         2601 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_446653,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000253 DASRDH010000000
VERSION     DASRDH010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446653"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..1390
                     /gene="clpX"
                     /locus_tag="VFA14_05860"
     CDS             119..1390
                     /gene="clpX"
                     /locus_tag="VFA14_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HZS80934.1"
                     /translation="MSEKKSSSGEKLLYCSFCSKSQHEVKKLIAGPSVFICDECIGLC
                     NDIIRDEASSIEPVADARSSLPTPQEISELLDQYVIGQQTAKRILSVAVYNHYKRLKH
                     LGKKDDVELTKSNILLVGPTGSGKTLLAQTLARMLNVPFVIADATTLTEAGYVGEDVE
                     NIIQRLLQSCNYEVEKAQKGIVYIDEIDKISRKSDSPSITRDVSGEGVQQALLKLIEG
                     TMASVPPQGGRKHPNQDFVQIDTTNILFICGGAFDGLAKIISERSEKSGIGFGASVSV
                     RSRSQEAAGAMLSHAEPDDLVKFGLIPELIGRLPVIATLSELDEAALIQILLEPKSAL
                     IKQYAKLLQMEGAELEIRPAALQAIARKALARKTGARGLRSILEQALLDVMYELPGQQ
                     NVAKVVIDENTIANNARPLLIYHDQPRVSGA"
     gene            1509..>2601
                     /locus_tag="VFA14_05865"
     CDS             1509..>2601
                     /locus_tag="VFA14_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008113665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HZS80935.1"
                     /translation="MTTAILTEQAQLPLLPLRDVVVFPHMVIPLFVGRPKSIKALEAA
                     MEPGRSIMLAAQKAAAKDEPSADDIYEIGCVANILQMLKLPDGTVKVLVEGAQRARIH
                     HISELDSHFVADLTPVESSLGDAAEVEAMRRTIVQQFDQYVKLNKKIPPEILSSLAGI
                     DDAGRLADTIAAHLPLKLEQKQVILEIFNVAKRYEHLLGQLEGELDILQVEKRIRGRV
                     KRQMEKSQREYYLNEQVKAIQKELGEGEDGADLEDLEKKILAAKMSREALDKAQSELK
                     KLKLMSPMSAEATVVRNYIDTLVGLPWKKKSRVNNELAHAEEILEGDHYGLEKVKERI
                     LEYLAVQQRVDKLKAPILCFVGPPGVGKTS"
BASE COUNT          621 a          665 c          743 g          572 t
ORIGIN      
        1 catatggaga aacaggcatt ttcaggtatg ctgaaagtgg ccaaacatgg tgattctgtg
       61 gtgcatcatg gaatcttggg tatgatcagg ccggatttca ttcacatttg tcttttctat
      121 gtccgaaaaa aaatcttcca gtggtgaaaa gttgctgtat tgctcgtttt gcagcaaaag
      181 ccagcatgaa gtcaagaaac tgattgctgg cccatcggtt ttcatttgtg acgaatgtat
      241 cggtctgtgc aacgatatca ttcgcgatga ggcttccagt attgaaccgg ttgctgacgc
      301 cagatccagt ctgccgacgc cgcaggagat ttctgaattg ctggatcagt acgtgatcgg
      361 ccagcagacc gccaagcgta tcctgtcggt cgccgtgtat aatcactaca agcgtctgaa
      421 gcatctcggc aagaaggacg acgtcgaact caccaagagt aatattctgc tggtcggtcc
      481 gactggttcg ggcaagacgc tgctggcgca gacacttgcg cgcatgctga atgtgccgtt
      541 cgtgattgcc gatgcgacca cgctgactga agctggttac gtcggtgaag atgtcgagaa
      601 catcattcag aggctgctgc aaagctgcaa ctatgaagtc gagaaggcgc agaaaggtat
      661 cgtttacatc gacgaaatcg acaagatctc gcgtaaatcc gatagtccgt cgattacccg
      721 tgacgtttcc ggcgaaggtg tgcagcaggc gctgttgaag ctgatcgaag gcaccatggc
      781 atcggtgccg ccgcagggtg gacgcaagca tcccaaccag gacttcgtgc agatcgatac
      841 gaccaatatc ctgttcattt gcggcggcgc gttcgatggc ctcgcaaaga tcatttccga
      901 acgctccgaa aagagcggta ttggtttcgg cgccagcgtc agcgtcagga gtcggtcgca
      961 ggaagcggct ggcgcgatgc tgtcgcatgc ggaaccggac gatctggtca aattcggtct
     1021 gattccggaa ttgatcgggc gcttgcctgt gatcgctacg ctcagcgaac tggatgaagc
     1081 agcgctgatc cagattttgc tggaaccgaa aagcgcgctg atcaagcagt atgccaagct
     1141 gctgcaaatg gaaggggcgg agctggaaat ccgtcctgcc gccctgcagg caatcgccag
     1201 aaaggcgctg gcgcgcaaga ctggtgcgcg tggtctgcgc tccatcctcg agcaggcctt
     1261 gttggatgtc atgtatgagc tgcccggcca gcaaaacgtg gccaaggtag tcattgatga
     1321 aaatacgatt gccaacaacg ccaggccatt gctgatctac catgatcaac ccagggtttc
     1381 aggcgcctga tctcgccggg ggtcttccga tcgtttggat gcaaggcgca agtttttgtt
     1441 ttcattttgg cttgcgctcc agtggctcgt gccaacatca ctcacagtaa tttttgacaa
     1501 gacttgccat gacgactgct attctcacag aacaagcgca attgccttta ttgccgttgc
     1561 gggatgttgt tgtcttcccg catatggtga tccctttatt cgttggtcgt ccaaagtcga
     1621 tcaaggcgct ggaagcagca atggagccag gcaggagcat catgctcgca gcccagaaag
     1681 ccgccgccaa ggatgagccg tcggcagacg acatctatga aattggttgc gttgccaata
     1741 tcctgcaaat gctgaaatta cccgatggta ccgtcaaggt tctcgtcgaa ggcgcgcagc
     1801 gcgcgcgcat ccaccacatc agcgagctgg acagccattt cgtcgctgac ctgacaccgg
     1861 ttgagtccag cctgggcgat gcagcagagg ttgaagccat gcgccgcact atcgttcagc
     1921 agttcgatca gtacgtcaaa ctgaacaaga agattccacc cgagattctg agttcgctgg
     1981 ctggcatcga tgatgccggt cgcctggccg ataccattgc cgcgcatctg ccgctcaagc
     2041 tcgagcaaaa gcaggtcatc ctggaaattt tcaacgttgc caagcgttac gagcatttgc
     2101 tgggtcagct ggaaggcgaa ctggatatcc tgcaggtcga gaagcgcatt cgtggccgcg
     2161 tcaagcgcca gatggaaaag tcgcaacgcg agtactacct gaacgaacag gtcaaagcca
     2221 tccagaagga actgggcgag ggcgaagacg gtgccgacct ggaagatctg gaaaagaaaa
     2281 tccttgccgc caagatgtcc agggaagctc tggacaaggc gcaatctgag ttgaaaaagc
     2341 tcaagctgat gtcgccgatg tcggccgaag ccacggtcgt gcgcaattac atcgacacac
     2401 tggtcggttt gccctggaag aaaaaatcca gggtcaacaa cgagctggcg catgctgagg
     2461 aaattctgga gggcgatcat tacggtcttg aaaaggtcaa ggaacgcatc ctggaatacc
     2521 ttgctgtgca acagcgtgtc gacaagctca aagcgccgat tctgtgtttc gtcggtcctc
     2581 caggcgtggg caagacttcg c
//
LOCUS       DASRDH010000254         9656 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1217915,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000254 DASRDH010000000
VERSION     DASRDH010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 9656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9656
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1217915"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1182
                     /locus_tag="VFA14_05870"
     CDS             <1..1182
                     /locus_tag="VFA14_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016836703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZS80936.1"
                     /translation="LNRDTLTVNAELTQVIEETTRAQQVIKIFGGQPYEKKRFHERAE
                     NLRRTTMRMTAAFSATVPITQVMTACAVAIVIVMALVQSGNGQITVGGFVSFLTAMLM
                     LQAPLKQLAEINGPLQRGMAAAEEVYLLIDKATERTGGETLATRSAGRLDLIDVDFSY
                     PGQRQLALRNINLSVQPGETIAFVGMSGGGKSTLVSLIPGFHSVTGGAILLDGKPIEA
                     ISLVSLRRQIAMVSQNTVLFDDSLAANIAYGDSQPDAERLHAAVVAAHLLDVVNDMPE
                     GLATRIGDNGSRLSGGQRQRVAIARAIYKDAPILILDEATSALDTESERTVQAALERL
                     MTGRTTLMIAHRLSTIERADRIVVLAHGQIVETGSHQQLLANEGVYAHLYRLQFSRQA
                     N"
     gene            1208..2320
                     /locus_tag="VFA14_05875"
     CDS             1208..2320
                     /locus_tag="VFA14_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HZS80937.1"
                     /translation="MLQTLIPAESLKKADKMLFITHLALGDFTYLQNFFHAFKRAHPQ
                     LKLHLWIDEVRRTSDATQWRHLEKYALYDWVAVCPCFDRIYTRTYSPALYQESLIAAR
                     QEHYPTVVSLATLRPHRYANLARSISPDGLVIGMKNLARFYQPHHSLAYRKLDAAILP
                     CRARCDDPQHISDIYAHWFRQISGLEVAATDRLPFVDIPAQWQQVAQEQLSTWGFRND
                     ATGRSKLVFINPYAKTRKRCWPLERVTALILAMKKQNQWCDSSFIVNAVPQELIQARR
                     VISSYRLERTELFSAEENFFQLPAILAQCDLIISVETAVMHLANAVHVPVIALMRQKN
                     PEWAPIDRANSTVITAPKRRDWVKAITVEQVMKVIP"
     gene            2317..3291
                     /locus_tag="VFA14_05880"
     CDS             2317..3291
                     /locus_tag="VFA14_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008116961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZS80938.1"
                     /translation="MNQQTRPAGSNGKPSLHIAFCVDNHYFRAMGGTIASIIDNNPGQ
                     HFTFHVLTFAVSDDHQRRLKRLEAMYPVSTRLHLLDPASFTQFSRFLGHSHYSLSIFT
                     RLVIPTVLQGQTDRVLYLDADILCVNRLDELVDMDISRDIAVVAPDAPVTRQRRVTAL
                     GLKHHAYFNGGVIFINIDKWIAENITGQTLDALENSKTDMRFNDQDALNIVLDGRARY
                     ISPRWNYLYDLIHDLNVNRFTLRPVGKAVFIHFAGAVKPWADWSGHDARHLFRKYLSL
                     SPWSDMPLDPEPKNTKEMRMRSRFLYRQRRPLESLTWYLRYLRRRATQ"
     gene            complement(3264..4535)
                     /locus_tag="VFA14_05885"
     CDS             complement(3264..4535)
                     /locus_tag="VFA14_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HZS80939.1"
                     /translation="MNKFTSVAVFLYSAISLIVPSGFSVGSGLLVLGSIVLLRERGVL
                     FLDREDRHLMAVFFIYFLVSTGMNVLHGEIIREYDLPLRFLLAIPALLLLRAYPPSPA
                     FFWAGLGIGGILAGLYTGWQNLFVLHTRAGGHTNPIQYGNISFIIGMLCLAGLGWAML
                     QQRARYWIWFLLCGFVMGMLGSLFTGSRGSWIGLPFCLYILYRCYGAEFGRRYVWGGI
                     AGVVLAIVVVFAIPRTEVRMRAELAVSESIEYLQTRNANSSIGARMELWRVGVRAAAE
                     HPLLGWGKAGFVAWENREMADGRVDPHISGFDHVHQEWLDALAKRGVLGLIVLLVLYA
                     LPLRLFSTFVRTAGKRARPYAIAGVLLFVNYICFGFSQVFLSHNSGVMTLAFTTVILW
                     GLLRAEASKENAQLPLHVSAVKNHCVARLRK"
     gene            complement(4673..5236)
                     /locus_tag="VFA14_05890"
     CDS             complement(4673..5236)
                     /locus_tag="VFA14_05890"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HZS80940.1"
                     /translation="MDAHLKESKVDGALVYEGHFLKVQRDIIRLPDGKQAAREYIRHP
                     GAVVILPLFDDGTVLLERQFRYPLDQVFIEFPAGKIDPDEDYLACARRELQEETGYTA
                     TDWRFVCTIHNAIAYSDEHLEMYLARGLIAGERKLDDGEFLDICKLPLAELLEQVRQG
                     RVTDVKTIIGAFWLEKIGSGQWSPSTA"
     gene            complement(5274..5588)
                     /locus_tag="VFA14_05895"
     CDS             complement(5274..5588)
                     /locus_tag="VFA14_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006712953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2818 family protein"
                     /protein_id="HZS80941.1"
                     /translation="MNVSLSSWCVILLALLAANLPFFNQQLFALVRLSSLPDYRKPVW
                     VCLLELIVLYFSVGVVAHVFEADIGHAFSQGWEFYVVTGCLFLVLAYPGYVARHLRKP
                     HA"
     gene            complement(5598..7109)
                     /gene="nuoN"
                     /locus_tag="VFA14_05900"
     CDS             complement(5598..7109)
                     /gene="nuoN"
                     /locus_tag="VFA14_05900"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HZS80942.1"
                     /translation="MNDMNLIPVYPEIFLLIATSAILLIDMFLPDAKRTITYLLSLLT
                     LAVCAALTFTCHAGDETVYTFNGMFVSDPLSYLLKLFSYLAVGLTLVYARRYNSDRAI
                     VGGAWLGGEFYALALFSLLGQMIMISGNSFLSIYLGLELMSLPLYALVALRRDDTGAT
                     EAAMKYFVLGALASGFLLYGISMLYGATGSLELSGVHRAIVSSTVDSSVLVFGIVFVV
                     AGLAFKLGAVPFHMWVPDVYHGAPTPMTLLIGGAPKLAAFAILFRLLVEGLLPLAIDW
                     QQMLMVLAVLSIIIGNVTAIAQTNIKRMLAYSAISHMGFMLLGMISGIVDGNLFQAIN
                     AYSSSLFYAITYVLITLGAFGVILLMTRSGFEADTLNDFKGLSQRSPWLAAVMLVLMF
                     SLAGIPPMVGFYAKLSVLQALLGAGQIWLVVLAVVFSLVGAFYYLRVIKNMYFDEPVD
                     NGRVSVGFDMRIALSLNGLTALVLGLWPGPLMDACTNAIVITLATFLSKVVAG"
     gene            complement(7180..8667)
                     /locus_tag="VFA14_05905"
     CDS             complement(7180..8667)
                     /locus_tag="VFA14_05905"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HZS80943.1"
                     /translation="MMSTFPFLSLAIWCPVVFGILVLAVARDENLAVARCLSLFGAVV
                     SFLVTVPLFMYFDAAAHGYQFVEQSLWIDRFNISYFLGIDGLSLWFIPLTAFITIIVV
                     LAGWEVIQKRVAQYMGSFLILSGLMVGVFSALDGVLFYVLFEATLIPMFIIVGVWGGA
                     NRVYAAFKFFLYTLLGSLLMLIALIYLYLVSGGNFDIPTWHRLPLSMLEQKWLFFAFL
                     VAFAVKVPMWPVHTWLPDAHVEAPTGGSIVLAAIMLKLGAYGFLRFSLPIAPDASHAL
                     SGFMITLSLIAVIYIGLVALVQQDMKKLVAYSSIAHMGFVTLGFFMFNDMALQGAIAQ
                     MISHGFVSGAMFMSIGVLYDRMHTRDIAWYGGVVNRMPRFAAFFILFSLANCGLPATS
                     GFVGEFMVILGAVEFNFWIGLLSATALILGAAYSLWLVKRVIFGAVTNHHVAELADVN
                     RREFFILGLLAVAVLAMGLYPAPVIDAMQTSVADLLEHVAVSKMQ"
     gene            complement(8710..>9656)
                     /locus_tag="VFA14_05910"
     CDS             complement(8710..>9656)
                     /locus_tag="VFA14_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007881965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HZS80944.1"
                     /translation="GSVIIGMHHDQDMRNMGGLRKYLPITWITSLLGSLALIGTPFFS
                     GFYSKDSIIEAAAASSLPGSGFAYFAVLASVFVTAFYSFRMYFMVFHGAERFGKAYDH
                     ARVHADAHAEHHGLAPGQKPHELPLVVTLPLILLAIPSVVIGFFAIAPMVFGDFFSGV
                     LFIGTNHKAIEDLAREFHGPLAMAVHALTSAPLWLAIAGVVAAYYCYMVDPRVPAWFK
                     DRFSAIYTLLDNKYYMDRFNEVVFAGGARWLGGALWSVGDKVLIDGLIVNGSARLVNW
                     FSKVTRLWQSGYLYHYAFVMIIGMLGFLVWFMPFPFAK"
BASE COUNT         2476 a         2999 c         2519 g         1662 t
ORIGIN      
        1 ctcaaccgcg atacgctcac cgtcaatgcg gaactgaccc aggtcatcga agaaaccacg
       61 cgcgcccaac aggtcatcaa gatcttcgga ggccaaccat acgaaaaaaa acgtttccat
      121 gaacgcgccg agaatctacg ccgtaccacc atgcgcatga cggccgcatt ttccgccacg
      181 gtgccgatca cgcaggtgat gacggcctgc gccgtggcga tcgtgatcgt catggcgctg
      241 gtccagtcgg gcaatggtca gatcaccgtc ggtggcttcg tctccttcct cacggcgatg
      301 ctgatgctgc aagcaccgct caagcagctg gctgaaatca acggcccgct gcagcggggc
      361 atggcggcgg ccgaagaagt ctatctcctg atcgacaagg ccaccgagcg taccggcggc
      421 gagacgctgg caacgcgcag cgctggcaga ctcgatctga tcgatgtcga tttctcttac
      481 cccggccagc gccagttggc gctgcgcaac atcaacctga gcgtgcaacc gggcgaaacc
      541 atcgcctttg ttggcatgtc cggcggcggc aaatcgacac tggtcagcct gattcccggc
      601 ttccattccg tcaccggcgg cgcaatcctg ctcgacggca agccgatcga agcgatttcg
      661 ctggtcagcc tgcgccggca gatcgccatg gtcagccaga ataccgtact gttcgacgac
      721 agtctggcag ccaacatcgc ctacggcgac agccagcccg atgcagagcg cctccatgcc
      781 gccgtcgttg ccgcgcacct cctcgacgtc gtcaacgaca tgccagaagg actggcaacc
      841 cgtatcggcg acaatggctc gcgcctgtcg ggtggccagc gccagcgtgt agcaatcgcc
      901 cgcgccatct acaaggacgc gcccatcctg attctcgatg aagcgacgtc cgcgctcgat
      961 accgaatccg aacgcaccgt gcaagccgcg ctggaacgcc tgatgacagg ccgtaccacc
     1021 ctcatgatcg cgcaccgcct gtccaccatc gagcgcgccg accgtatcgt cgtgctggcg
     1081 catggacaaa tcgtcgaaac cggcagccac cagcaattgc tggccaatga gggcgtgtac
     1141 gcccatctct accgtttaca gttctctcga caagcgaact gacgccatca cacagtaaca
     1201 gccagccatg ctccagacac tcattcccgc ggaatcactg aaaaaagcgg acaagatgct
     1261 attcatcacg catctggcgc tgggggattt cacttatctg caaaatttct tccacgcctt
     1321 caagcgcgcc catccgcagc tgaagctgca tctttggatc gatgaagtgc ggcgcaccag
     1381 cgatgccacg caatggcggc atctcgaaaa atatgcgctg tacgactggg tcgctgtctg
     1441 cccctgcttc gacagaatct atacccgcac ctacagcccg gcgctctacc aggagtcgct
     1501 catcgcagcg cggcaggaac actatcccac cgtcgtgtca ctggcgacgc tgcgcccgca
     1561 tcgctatgcc aacctggcac gcagcatcag cccggacgga ctggtcatcg gcatgaagaa
     1621 cctcgccagg ttctaccaac cgcatcatag cctggcctat cgcaaactgg atgccgcgat
     1681 tcttccctgc cgcgccaggt gcgacgatcc gcagcacatc agcgatatct atgcgcactg
     1741 gttccgtcag atcagtggac tggaagtcgc tgccacggac agattgccct ttgtcgatat
     1801 tccagcgcaa tggcaacagg tcgcgcagga gcaattatcg acatggggct tcaggaacga
     1861 tgccactggc cgcagcaaac tggtgttcat caacccctac gccaaaacca gaaaacgctg
     1921 ctggccgctg gagcgcgtca ccgcgctgat cctcgccatg aaaaagcaga atcagtggtg
     1981 cgacagcagc ttcattgtca acgccgtgcc gcaggaactg atacaggcgc gccgggtgat
     2041 cagcagctac cgtctggaac gcaccgaact gttcagcgcc gaagaaaatt tcttccagct
     2101 gccggcaatc ctggcgcaat gcgacctcat catctcagta gaaaccgccg tgatgcatct
     2161 ggccaacgca gtgcatgtgc cagtaatcgc gctgatgcgt caaaaaaacc cggaatgggc
     2221 accgatcgac cgcgccaaca gcactgtcat caccgcgccg aagcgccgcg actgggtcaa
     2281 ggccatcacc gtcgaacaag tcatgaaagt cataccatga atcagcaaac caggcccgcc
     2341 ggcagcaacg gcaaaccatc cttgcatatc gccttttgcg tcgacaacca ctatttccgc
     2401 gcgatgggcg ggacgatcgc ctccatcatc gacaacaacc cgggacagca cttcactttt
     2461 cacgtactga cgttcgcagt ctccgacgat catcagcgcc gcctgaaacg tctggaagcg
     2521 atgtacccgg tcagcacacg attgcacctg ctcgatccgg cgtcgttcac gcagttctcg
     2581 cgttttctcg gccattcgca ctactctctg tcgatcttta cgcggctggt gatcccgacc
     2641 gtgctgcaag gccagaccga ccgcgtgctg tatctcgacg ctgacatcct gtgcgtcaac
     2701 cggctcgatg aactggtcga tatggacatc agtagggata tcgccgtagt ggcgccggac
     2761 gcgccggtga cgcggcaacg ccgtgtcacc gcgctcggcc tgaaacacca tgcctatttc
     2821 aacggcggcg tgatcttcat caacatcgac aagtggatcg ctgaaaacat caccggacag
     2881 acgctggatg cgctggagaa tagcaaaacc gacatgcgct tcaacgatca ggatgcgctc
     2941 aatatcgtgc tcgatggccg cgcgcgctac atctcgccgc gctggaatta tctatacgac
     3001 ctgatccacg acctgaatgt caaccgcttc accctgcgcc cggtcggcaa agccgtgttc
     3061 attcacttcg ccggcgccgt caaaccctgg gcggactgga gcggccacga cgcacgccac
     3121 ctgttccgca agtatctatc gctgtcgcca tggtcggaca tgccgctcga tcccgagccg
     3181 aaaaacacca aggaaatgcg catgcgctcg cgcttcctgt accgccagag acgaccactg
     3241 gagagcctga catggtatct ccgctatttg cgcaggcgcg ctacacaatg attcttcacg
     3301 gcagaaacgt gcagcgggag ctgcgcattt tcctttgacg cttcggcgcg caacagcccc
     3361 cacaagatca cggtagtaaa cgcaagcgtc atcacaccgc tattgtgcga caggaacacc
     3421 tgcgaaaaac cgaaacagat atagttgacg aacagcagca cgccagcgat ggcatacggc
     3481 ctggcgcgct ttcctgcagt tctgacaaac gtactgaata gacgcaatgg cagcgcatac
     3541 aggaccagca aaacaatcaa acccaacacg ccccgtttgg caagcgcatc cagccattct
     3601 tgatggacgt gatcaaatcc ggatatatga ggatcaactc tgccgtccgc catttcacga
     3661 ttttcccagg caacgaaacc agctttaccc caccccaaca gtgggtgttc agcggcagcc
     3721 ctcaccccca cgcgccacag ctccatgcgc gcgccaatgg aagaattggc attcctcgtt
     3781 tgcagatact cgatactttc actgaccgcc aattctgcgc gcatcctgac ctcggtgcgg
     3841 ggaatcgcga aaaccactac gatcgcaagg acgacgccgg caataccccc ccagacatag
     3901 cgcctgccga attcagcgcc gtaacaacgg tatagtatgt acagacaaaa cggcaagcca
     3961 atccagctgc cgcggctacc cgtgaataaa gacccgagca tgcccatgac aaaaccacac
     4021 agcaaaaacc agatccaata gcgcgcacgc tgttgcaaca tggcccaccc cagccctgca
     4081 aggcacaaca taccgataat gaaactgatg ttgccgtact gaatcggatt agtatgaccg
     4141 ccagcccgag tgtggagaac aaataaattc tgccatccgg tatacagtcc ggccagaata
     4201 ccgccaatgc cgagaccggc ccagaaaaaa gcaggagacg gtggataagc ccgcagcagc
     4261 agcagcgccg gaattgccag caaaaagcgc aagggcaaat catattccct gatgatttcc
     4321 ccatgcagca cattcattcc tgtcgacacc agaaaataaa tgaaaaacac tgccatcaaa
     4381 tgcctgtctt cccgatccag aaaaagaacg ccccgctccc tcaatagcac gatgctgccc
     4441 aacaccagca aaccggagcc aaccgaaaac ccgctcggaa caatcagaga aatagccgaa
     4501 taaagaaaaa cggcaaccga ggtaaattta ttcatcaaga tagtttttta ctgtatttaa
     4561 gcggctacac atggctttcc aataaccacg cacgatacct cacctttcgc gccgccggct
     4621 tggtcgttat caatccctgg acatggaaag cggcgccatg aacacctatg attcaagctg
     4681 tggacggact ccattggcca ctgcctattt tctccagcca gaaggcgccg atgatggttt
     4741 tgacatcagt gacccgacct tgccggacct gctccagcaa ttcggccaat ggcagcttgc
     4801 agatgtcgag gaattcaccg tcatcgagtt tgcgctcgcc agcaatcaat ccgcgcgcca
     4861 gatacatctc cagatgctca tctgaatagg caatggcatt gtggatggtg cagacgaatc
     4921 gccagtcagt cgcggtgtag ccggtttctt cctgtaattc acgcctggcg caggccagat
     4981 agtcttcgtc gggatcgatc ttccctgccg ggaattcgat gaaaacctga tccagcggat
     5041 aacggaactg acgttccagc agtacagtgc catcatcgaa caagggtagg atcaccaccg
     5101 cgccagggtg cctgatgtat tcacgcgccg cctgtttgcc atccggcagg cgaatgatgt
     5161 cacgctggac tttgagaaaa tggccttcgt agaccagtgc gccgtctact ttgctttctt
     5221 tgagatgagc atccatgaca agggtcgata atcaatcaac aacggcacac cattcaggcg
     5281 tgaggcttgc gcaagtggcg cgccacatag ccgggatacg ccagcaccag aaacaggcag
     5341 ccggtcacga catagaattc ccagccctgg gaaaatgcat gaccgatgtc ggcttcgaag
     5401 acatgcgcaa ccacgccgac gctgaaatac agcacaatca gctccagcag gcacacccat
     5461 accggcttac ggtaatcggg caaactgctc aatcgaacca gcgcaaacag ctgctgattg
     5521 aagaaaggca ggttggcagc cagaagcgcc agcaggatga cacaccagct cgataaagaa
     5581 acattcaccg gtaatactca ccccgcgacg actttgctga ggaaagtcgc cagcgtgatg
     5641 acgattgcat tggtacaggc atccatcaaa ggcccaggcc acaggccaag caccagcgct
     5701 gtcagcccat tcaggctcaa agcgatacgc atgtcgaagc cgacgctgac cctgccgttg
     5761 tcgacaggtt catcgaagta catattcttg atcacacgca ggtaatagaa tgcgcctacc
     5821 agcgagaaca cgactgccag caccaccagc cagatctggc ccgcgcccag cagcgcctgc
     5881 agtaccgata gcttggcgta aaaaccgacc atgggcggaa tacccgcgag cgaaaacatc
     5941 aggaccagca tcacggcggc gagccatgga ctgcgctggc tcaatccttt gaaatcattc
     6001 agcgtatcgg cctcgaaacc ggaacgtgtc atcagcagga tcacaccgaa ggcgcccagc
     6061 gtgatcagca cataagtgat cgcataaaac agcgaagaac tgtaggcatt gatagcctgg
     6121 aacagattgc catcgacgat accggagatc atgcccagca gcatgaaacc catgtgcgag
     6181 atggccgagt acgccagcat gcgcttgata ttagtctgcg caatagccgt tacgttaccg
     6241 atgatgatgg acagcaccgc cagcaccatc agcatttgct gccagtcgat cgccagcggc
     6301 aacagtcctt ccaccagcag gcggaacagg atggcgaacg ccgccagttt cggcgctccg
     6361 ccgataagca gcgtcatcgg cgtcggcgcg ccatgatata cgtcaggcac ccacatgtgg
     6421 aacggcacag cacccagttt gaacgccaga ccggcaacca cgaatacgat accgaacacc
     6481 agcacgctgc tgtcaaccgt gctggatacg atcgcgcggt ggacgccact cagctccagc
     6541 gagccggtgg cgccgtacag catggaaata ccgtataaca gaaagccgga agccagcgcg
     6601 ccaaggacga agtatttcat cgcagcttcg gtcgcgccgg tatcgtcgcg ccgcaacgcg
     6661 accagcgcgt ataacggtag cgacatcagc tccaggccga ggtaaatgga gagaaaacta
     6721 ttgccggaaa tcatgatcat ctggcccagc agcgagaaca gcgccagcgc gtagaactca
     6781 ccacccagcc aggcgccgcc gacaatggca cggtcactgt tgtatcgacg cgcgtaaacc
     6841 agtgtcaggc cgacagccag ataagagaac agcttcagca gatacgacag aggatccgac
     6901 acgaacatgc cgttgaaggt atacaccgtc tcatcaccgg cgtgacaggt gaaagtcaac
     6961 gccgcgcaca ccgccagcgt cagcaaagac agcaaatagg tgatggtgcg ttttgcatcc
     7021 ggcagaaaca tgtcgatcaa cagaattgcg gaagtcgcaa tcagcagaaa gatttccgga
     7081 taaacaggga tcagattcat gtcattcatt cagttaacgc ttatttcgtc tgcgtatttc
     7141 attcgcttgc cgctgccgcc gttttcagta ctggacgact cactgcatct tggatactgc
     7201 tacgtgttcg agcagatccg ccaccgaagt ctgcatcgca tcgatcaccg gagccggata
     7261 caggcccatg gccagaacag caaccgccaa caggccgagg atgaagaatt cgcgcctgtt
     7321 cacgtcggcc agttcagcca cgtgatggtt ggtcaccgcg ccgaaaatca cgcgcttgac
     7381 cagccacagc gagtacgccg cacccaggat caaggccgtc gccgacaaca gaccgatcca
     7441 gaaattgaac tcaacggcgc cgaggatcac catgaattcg ccgacgaaac cggaggtagc
     7501 cggcagaccg cagttggcca gcgagaacag gatgaagaat gccgcgaagc gcggcatcct
     7561 gttcacgaca ccaccatacc aggcgatgtc acgcgtatgc atgcggtcgt acagcacgcc
     7621 aatcgacatg aacattgcgc cggacacgaa gccatgcgaa atcatctgcg cgatagcgcc
     7681 ctgcaacgcc atgtcgttga acatgaagaa gcccagcgta acgaatccca tgtgcgcaat
     7741 cgacgaatac gcgaccagtt tcttcatgtc ctgctgcacc agcgcgacca gaccaatgta
     7801 gatcacggcg atcaacgaca gcgtgatcat gaaaccggac agcgcatggc tggcgtcagg
     7861 cgcgatcggc agcgagaacc gcagaaagcc gtaagcgccg agtttgagca tgatcgcggc
     7921 cagcacgatg gaaccgccag tcggcgcttc cacgtgggca tccggcaacc aggtatgcac
     7981 cggccacatc ggcaccttga cggcaaacgc caccaggaag gcaaagaaca gccatttttg
     8041 ttccagcatc gacagcggca gccgatgcca tgtcggaatg tcaaagttgc cccctgatac
     8101 gagatacaga tagatcagcg caatcagcat cagcagcgag ccgagcaagg tgtataggaa
     8161 gaatttgaac gctgcataca cgcggttggc gccgccccac acacccacga tgatgaacat
     8221 cggaatcagg gtcgcttcga agagcacata gaacagcacg ccgtcgagcg cggaaaacac
     8281 accgaccatc aaaccagaca gaatcaaaaa agatcccatg tactgcgcga cacgcttttg
     8341 tatcacttcc cagccggcca gcaccacgat gatggtgatg aaggcggtca gcggaatgaa
     8401 ccacagcgac agaccatcga taccgaggaa ataactgatg ttgaagcggt cgatccacag
     8461 cgactgctcg acaaactggt agccatgcgc cgccgcatca aaatacatga acagcggcac
     8521 ggtcaccagg aaactgacaa cggcgccgaa cagggataag cagcgcgcga cggccagatt
     8581 ttcgtcgcgg gcgacagcca gaacgaggat accgaatacg accggacacc agatcgcaag
     8641 actcaggaaa ggaaaagtgg acatcatggt cgttagagcg tgttatggac tatttacttt
     8701 gttgccaact catttggcaa acgggaacgg catgaaccag accagaaaac ccagcatccc
     8761 gatgatcatc acaaaagcgt aatgatagag atagccggac tgccacaggc gcgtgacttt
     8821 cgagaaccag ttgaccagcc tggcgctgcc gttgacgatc agaccgtcga tcaagacctt
     8881 gtcgccgaca gaccacagag cgccgcccag ccagcgcgcg ccgccggcaa acacgacttc
     8941 attgaaccta tccatgtagt acttgttgtc cagcagcgtg tagatcgcgc tgaatctatc
     9001 cttgaaccag gccggcacgc gcggatcgac catatagcag tagtacgccg cgacgacgcc
     9061 ggcaatggcc agccatagcg gcgcggatgt cagcgcatgc acagccatcg ccagcggacc
     9121 atggaattcg cgggccagat cttcgatggc cttgtggttt gtaccgatga aaagcacacc
     9181 gctgaaaaaa tccccgaaaa ccatcggcgc aatggcgaag aaaccgatga cgaccgatgg
     9241 aatcgccagc aggatcaacg gcagcgtcac caccagcggc aactcgtgcg gcttctggcc
     9301 cggcgccagg ccatggtgtt cggcatgcgc atcagcgtga acgcgcgcat gatcatacgc
     9361 cttgccaaaa cgctctgcgc catgaaacac catgaaatac atgcggaacg agtagaacgc
     9421 cgtcacgaac acgctggcca acaccgcgaa gtaagcaaaa ccggaacccg gcagagacga
     9481 tgcggcagcg gcttcgatga tactgtcctt cgagtaaaag ccggaaaaga agggcgtgcc
     9541 gatcagcgcc agcgacccca gcagcgaagt gatccaggtg atcggcaggt acttgcgcag
     9601 gccgcccata ttgcgcatat cctgatcatg atgcatgcca atgatgaccg aacccg
//
LOCUS       DASRDH010000255         9967 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1080114,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000255 DASRDH010000000
VERSION     DASRDH010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 9967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9967
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1080114"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            160..1002
                     /gene="truA"
                     /locus_tag="VFA14_05915"
     CDS             160..1002
                     /gene="truA"
                     /locus_tag="VFA14_05915"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007884174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HZS80945.1"
                     /translation="MRRIVFGIQYDGNAWQGWQTQPHGRTVQDRLEAALKQFTLTGIA
                     TTCAGRTDSGVHALEQVVHFDTDIARDKFSWVRGVNTFLPASIAVRWACELSIEDGPA
                     RSVAEREGGFHARFSATARTYHYLLYNHPVRSPLLVGKAGWTFRPLDVTLMQQAANYL
                     VGEHDFTTFRAVECQAKSPVRIMESLQVRRYGDLIVFTLKANAFLHHMVRNIVGSLIF
                     VGNGNQPPQWIAQVLAQKDRSRAAPTFMPDGLYLIKVDYPAHWALPQEGTAWPWCQPA
                     LHIG"
     gene            1038..1700
                     /locus_tag="VFA14_05920"
     CDS             1038..1700
                     /locus_tag="VFA14_05920"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HZS80946.1"
                     /translation="MMHRTRIKICGLARAADIAAAVAAGADAIGFVFYAGSPRHVTAS
                     AAAKLARQIPPFISTVGLFVNAGAEEVCAVLEEAPLSLLQFHGDETATQCAAIAHAVK
                     RPFIRAARIGSATTQADLIEYEQDYRSASPFFTGLLLDTLVEQYGGSGKVFDWSLIPE
                     ELASRAVLSGGLSVKNAADAVRRVRPHAVDISSGVERAKGIKDAAKITAFIEAVRRAD
                     DS"
     gene            1754..2980
                     /gene="trpB"
                     /locus_tag="VFA14_05925"
     CDS             1754..2980
                     /gene="trpB"
                     /locus_tag="VFA14_05925"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008330854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HZS80947.1"
                     /translation="MKASKLRQAASYGLPDARGHFGQYGGSFAAETLTHALTELREAY
                     AHYQHDVQFLAEFHTELKHFVGRPSPVYHAKRWSELAGGAQLYFKREDLNHTGAHKIN
                     NVIGQALLARRMGKQRIIAETGAGQHGVATATICARFGMECIVYMGSEDVKRQLQNVY
                     RMNLLGASVVSVESGSRTLKDAMNEAMRAWVTHVETTFYIIGTVAGPHPYPMMVRDFQ
                     SVIGNECLVQMPEIASRQPDYVVAAVGGGSNAMGIFYPYIDYQDVKLVGVEAAGDGLD
                     TGRHAASLTLGSPGVLHGNRTYLLQDENGQIMETHSVSAGLDYPGVGPEHAWLKDSGR
                     ASYVCITDDEALAAFHTCCRIEGIIPALESSHALAYAARLATTLSKDKIVLVNLSGRG
                     DKDMHTVQQRQGGPWK"
     gene            3033..3830
                     /gene="trpA"
                     /locus_tag="VFA14_05930"
     CDS             3033..3830
                     /gene="trpA"
                     /locus_tag="VFA14_05930"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008111952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HZS80948.1"
                     /translation="MSRIQSTFSTLAASNKKALVTFITAGDPAPEFTVPLLHALVAGG
                     VDILELGVPFSDPMAEGPVIQRACERALKFNVSTRDVLGYVREFRKTDMVTPVVLMGY
                     ANPLERFGIDAFIAAAKEAGVDGTIVVDYPPEECEEFAQKMQTQGLDPVFLLAPTSTD
                     ERIQQVAKVSSGFCYYVSLKGVTGAGNIDIAEVAARVAALRQHIKLPIGVGFGIGDGA
                     TVRAVSQVADAVVIGSRLIQELENTPHERVTEAAQTFVADIRRVMDA"
     gene            3982..4854
                     /gene="accD"
                     /locus_tag="VFA14_05935"
     CDS             3982..4854
                     /gene="accD"
                     /locus_tag="VFA14_05935"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012403665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HZS80949.1"
                     /translation="MSWLEKLLPPQIQRSDSASRKSVPEGLWVKCPSCEAVLYRTDLE
                     SNLHVCPKCSHHMRIRARARLDALLDEGGHYEIGQEALPVDTLKFKDSKKYTDRLKDA
                     LESTGETDAMVVMGGAIMTLPVVVACFEFEFMGGSMGSVVGERFVRGTQVALEQKVPF
                     ICITATGGARMQEGLLSLMQMAKTTAMLTRLSEKKLPFISVLTDPTMGGVSASFAFMG
                     DIVMAEPKALIGFAGPRVIENTVREKLPEGFQRAEFLVTKGAVDMIVDRRKMREEIAH
                     LLALLQNQAAETIS"
     gene            4957..6258
                     /gene="folC"
                     /locus_tag="VFA14_05940"
     CDS             4957..6258
                     /gene="folC"
                     /locus_tag="VFA14_05940"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007884169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HZS80950.1"
                     /translation="MQAQVSPVTLADWLALLETRHVKAIDMGLERVGKVKERLGIRFD
                     CPLITVAGTNGKGSTCAMLESILIRAGYRVGLYIKPHFLYFNERARVAGTPVSDAALI
                     AVFEAVEAVRGDISLTYFEFTTLAIMKLLADARLDVVILEVGLGGRLDAVNVIDADVG
                     IITSIDIDHTGYLGSTREEIGLEKAGIYRAGKTAICGDPMPPKSLVRYAEAIGADLWL
                     MGRDFNYMGDRLQWAYGGRAQRRNSLAYPSLRGANQLLNATAALAALEALRDRLPVGA
                     QEVRAGLATVALPGRFQVLPGQPLVILDVAHNPHAAATLAQNLDNMGFHPYTYAVFGC
                     MLDKDIKGIIGQLEGKIDHWCVTGLPLPRAATAQQLKDKLLEAGIVPECGSNAACTIT
                     SFDTPAAALANAQSRAGENDRIVVFGSFLTVAGVMEARKPK"
     gene            6389..7114
                     /locus_tag="VFA14_05945"
     CDS             6389..7114
                     /locus_tag="VFA14_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HZS80951.1"
                     /translation="MRSARKLAARRRRVGKEDTADPALSEKKRARRRLIGVVALVLAM
                     IIVLPMILDSEPRPLADDISIQIPSRSSVADARPAALAPAAEQPTAVPVTEKTEVDAK
                     VDTKADVKEGRAEAKMADRLAGHQDDHRMEKDAGETSKPKTAWKPDDDARAVAILEGR
                     SVVPAGGAFVIQVAVLTTQEKIDELRNKLSSASIKSYTQRVAIQSGEGVRIRVGPFAN
                     RHEADKMRARINKLGLNATIVPV"
     gene            7235..7723
                     /locus_tag="VFA14_05950"
     CDS             7235..7723
                     /locus_tag="VFA14_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016833455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HZS80952.1"
                     /translation="MTIFDYLVFFVLICSVSISILRGLVREVLSLASWTTAFVVAGAY
                     AEALAVMLPDGIPGASTKWIVGFIALFIGVRLLMALLGRAVREMVKVSGLSLIDRSLG
                     ALFGLARGAVIVMAVVLLCGTTSMPQQPFWKDALLSSQAVSAAQTVKPYLPGQFAQHI
                     HF"
     gene            7750..9267
                     /gene="purF"
                     /locus_tag="VFA14_05955"
     CDS             7750..9267
                     /gene="purF"
                     /locus_tag="VFA14_05955"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HZS80953.1"
                     /translation="MCGIVGVVSHSPVNQLLYDALLLLQHRGQDAAGIATNHANGFSM
                     HKANGLVRDVFRTRNMRSLPGNTGLGQVRYPTAGSSSSEEAQPFYVNAPFGIILAHNG
                     NLTNAEQLKVEMFRNDRRHLNTDSDTEVLVNVFAHELQQATTSYSLDPAAVFKAVAMV
                     HKRVRGSYAVVAQIAGYGLLGFRDPYGIRPMCLGYNETDKGHEYMLASESVALEGLGF
                     RFLRDVMPGEAIFIDQDGQLYNQQCAENPTLNPCAFEFVYLARPDSVLDGASVYATRL
                     KMGEFLAEKVRREIAGGEIDVVMPIPDSSRPAAMELALKLNLQYREGFIKNRYIGRTF
                     IMPGQSLRKKSVRQKLNAISAEFKGKNVLLVDDSIVRGTTSREIVQMAREAGARRVIF
                     ASAAPPVKFPNVYGIDMPTRDELIAYGRTDAEVCREITADALVYQDVEALKRSISDVN
                     PELKNFEASCFDGNYPTGDISRDYLDRIEFVRKNPKPALDDAMSSQLNLNLANVD"
     gene            complement(9283..>9967)
                     /locus_tag="VFA14_05960"
     CDS             complement(9283..>9967)
                     /locus_tag="VFA14_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007877970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HZS80954.1"
                     /translation="IGGGMAVQKLIADKWRQITGCAIIEGYGLSETSPIASANPYTIS
                     EYTGSIGLPLPSTEIRIVNDNGQPVASGQPGEIAIRGPQVMAAYWNRPDETRKVMTPD
                     GFFKSGDIGIMDERGYTRIVDRKKDMILVSGFNVYPNEIEEVATAHPGVLECACVGIP
                     DEHTGEAVKLFVVCRDASLTIEDLVVYCRERLTGYKQPKYIEFRTELPKSNVGKILRR
                     ELRDGKKIV"
BASE COUNT         2034 a         2708 c         2956 g         2269 t
ORIGIN      
        1 agaagtcata cgtggcggta gcgataccca ggtggggcgt gccaaggata tgttgtccaa
       61 gctaagctga gatcgtggac agggtacccg tttgttttta tccccacatg aacggtgatc
      121 gtgaccgagt cttcatcatt atccgcctcc atccggcgta tgcgccggat cgtgtttggc
      181 attcagtacg acggcaacgc ctggcaaggc tggcaaaccc agccgcacgg ccggactgtg
      241 caagacagac tggaagccgc gctcaagcaa ttcacactga ccggcattgc tacgacctgc
      301 gccggtcgca ctgattccgg cgtgcatgca ctggagcagg tggtgcattt cgataccgac
      361 attgcgcgcg acaaattctc gtgggtgcgt ggcgtcaata cttttctgcc ggcttccatc
      421 gccgtgcgct gggcttgcga gttgtcgatc gaagatggtc ctgcgcgcag cgtcgcggaa
      481 cgcgagggcg gttttcatgc gcgcttcagc gcgaccgcgc gtacctatca ttacctcctg
      541 tacaaccatc cggtgcgttc acctttgttg gtcggcaagg ctggctggac cttccgtccg
      601 ctggatgtga ctctgatgca gcaagcggca aattatctgg tcggcgaaca tgatttcacg
      661 acgtttcgcg ccgtagaatg ccaggcgaaa tcgccggtgc gtatcatgga gtctttacag
      721 gtgcgtcgtt atggcgattt gatcgtgttc actctgaaag ccaatgcctt tttgcatcac
      781 atggtgcgca atatcgtcgg ttcgctgatc tttgtcggca atggcaacca gccgccgcaa
      841 tggattgccc aggtgctggc gcagaaggat cgcagccgcg ccgcgccgac gttcatgccc
      901 gatggtctgt atctgatcaa agtcgattat ccggcgcact gggcgctgcc acaggaaggc
      961 accgcatggc cctggtgcca gccggctttg catatcggtt agactgagag cgctttctct
     1021 ttgacgactc cctggtaatg atgcatagga ccagaatcaa gatttgcggt ttggctcgcg
     1081 ccgcagacat tgctgctgcc gtcgctgccg gcgcggatgc gatcggcttc gtgttttacg
     1141 ccgggagtcc gcgccatgtc accgcgtcgg cggcggcaaa gttggcgcgc cagataccgc
     1201 ccttcatttc gacggtggga ttgttcgtca atgctggtgc ggaagaagtt tgcgccgtgc
     1261 tggaagaggc gcccttatcg ctactgcaat tccatggtga cgagacagcg acgcaatgcg
     1321 ccgccattgc gcacgctgtc aaacggcctt tcatacgcgc tgcacgcatt ggaagcgcaa
     1381 ccacgcaggc ggatttgata gaatacgaac aggattatcg tagcgccagc cctttcttta
     1441 cgggactctt gctcgacacc ctggttgagc aatacggcgg cagcggaaag gttttcgatt
     1501 ggtctctcat tccagaagaa ctcgcgtctc gggccgtttt aagtggtggc ttgagcgtga
     1561 aaaacgcggc tgacgcggtg cggcgcgtgc gtccgcatgc cgtcgacatc agcagcggcg
     1621 tcgaacgagc caagggcatc aaggatgccg ccaaaatcac cgcttttatc gaggcggtgc
     1681 gtcgcgcgga cgactcctga gctgggcagg ccggatcgtc cagtcgtctc accaggacat
     1741 gagaggaata tccatgaaag catcgaagct gcgtcaggca gcgtcttacg gcctgcctga
     1801 cgcgcgcggc catttcggcc aatatggcgg cagtttcgca gcggaaacgc tcacccatgc
     1861 cttgaccgaa ttgcgcgaag cctatgcaca ttatcagcat gatgtgcagt ttctggccga
     1921 atttcatacc gaactgaaac atttcgtcgg tcgtccgagt ccggtgtatc acgccaagcg
     1981 ttggtctgaa ttagccggcg gtgcgcaact ctactttaag cgcgaagacc tcaatcacac
     2041 cggcgcgcac aagatcaata acgtcattgg tcaagccctg ctggccagac gcatgggcaa
     2101 gcagcgcatc atcgccgaaa ctggcgccgg tcagcatggt gtggcgacag caacgatctg
     2161 cgcgcgtttt ggcatggaat gcatcgtcta tatgggcagc gaagacgtca aacgccagct
     2221 gcaaaacgtc taccgcatga acctgctggg cgccagtgtt gtgtcggtcg agtccggttc
     2281 caggacgctc aaggatgcga tgaacgaagc gatgcgcgcc tgggttacgc atgtcgagac
     2341 gacgttttac atcatcggta ccgtcgccgg cccgcatcct tatccaatga tggtgcgtga
     2401 ttttcagtcg gtgatcggca acgagtgtct cgtgcagatg cccgagattg caagccgtca
     2461 gccggactat gttgtggcgg ctgtcggtgg cggttccaac gcaatgggga ttttttatcc
     2521 ctacatcgat tatcaggacg tcaagctggt tggcgtcgag gcggcaggcg acggtcttga
     2581 taccggtcgt catgcggcct cgctgacgct gggttcgccc ggcgtgctgc atggtaatcg
     2641 cacctacctg ttacaggatg aaaacggtca gatcatggag acgcattcgg tctcggctgg
     2701 tctcgattat cccggtgtcg gtcctgagca cgcctggctg aaagacagtg gccgcgccag
     2761 ctatgtatgc atcaccgacg acgaggcgtt ggcggcgttt catacctgct gccgtatcga
     2821 aggtatcatc ccggcgctgg aatcgagtca tgcgctggcg tatgccgcca ggctagccac
     2881 gactctgtcc aaggataaga tcgtgctggt caatctgtcc ggccgcggcg acaaggacat
     2941 gcatactgtc cagcagcggc agggtggacc ctggaaatga ttgttaccgc atgtccaggt
     3001 gaaatgtttt ttcttttgag tattcgagca tcatgtcgcg tatccaatct actttttcaa
     3061 cgctggccgc aagcaataag aaagcgctgg tcactttcat taccgccggc gatccggcgc
     3121 cggaattcac cgtgccgcta ttgcatgcgt tggtcgccgg cggtgtcgat attcttgaac
     3181 tcggcgtgcc gttttccgat cccatggcgg aaggtccagt gattcagcgt gcctgcgagc
     3241 gcgccctgaa attcaatgtt agtacacgcg acgtactggg ctatgtgcgt gagttccgca
     3301 agactgacat ggtcacgccg gtggtcttga tgggctacgc caatccgctt gaacgcttcg
     3361 gcatcgatgc cttcattgcc gcggcgaaag aagccggcgt cgatggcact atcgtcgtcg
     3421 attacccgcc ggaagagtgt gaagagtttg cgcaaaagat gcagacgcag ggtctggatc
     3481 cggttttcct gctggcgccg acttcgacgg acgaacgcat acagcaagtg gccaaggtca
     3541 gcagtggttt ttgttactac gtgtccttga agggtgtaac cggcgccggc aatatcgata
     3601 tcgccgaagt ggcggcgcgt gttgctgctc tccgtcagca tatcaaattg ccgatcggcg
     3661 tcggtttcgg tattggcgat ggtgccactg tcagggctgt tagccaggtc gccgatgcgg
     3721 tggtgattgg cagtcgcctc attcaggaac tggagaatac gccgcacgag cgtgtaacgg
     3781 aagcggcgca gactttcgtg gcagacatcc gcagggtgat ggatgcataa gctgtaaaca
     3841 caggatgccg gcgaccagct ggcgcgtttc tgaactgaag agtgagcagg gagttgtcgc
     3901 ggggaaattg ccaaaaggga attgccgaaa ctttatcgaa taagcggtac aatgcgccac
     3961 cggaaattaa acgaggtttc tatgagctgg ctagagaaat tactcccgcc tcagattcaa
     4021 cgcagcgact ccgcgagccg caagtcggta cccgaaggtt tatgggtcaa atgtccgtcc
     4081 tgcgaagcag tactctaccg tactgatctg gagtccaatc ttcacgtttg tcccaaatgc
     4141 agtcatcaca tgcgtatccg cgcccgtgcg cgcctggacg cattgctcga tgaaggcggc
     4201 cattatgaga ttggccagga agctctgccg gtcgacacgc tcaagttcaa ggatagcaaa
     4261 aaatataccg accggctgaa agacgcactg gaatctaccg gtgaaaccga cgccatggtc
     4321 gtcatgggtg gtgccatcat gaccctgcca gtcgtggttg cctgcttcga attcgaattc
     4381 atgggtggct ccatgggttc tgtggttggc gagcgtttcg tccgcggtac gcaagtggcg
     4441 ctggagcaaa aagtgccgtt catctgcatc accgctaccg gcggtgcgcg tatgcaggaa
     4501 ggcttgctgt cattgatgca aatggccaag accacggcca tgctgaccag gctgtccgaa
     4561 aaaaagctgc cgttcatcag tgtgctgacc gatccaacca tggggggggt gtcggcatca
     4621 tttgccttca tgggcgacat cgtcatggcc gaaccaaaag cgctgatcgg ctttgccggt
     4681 cctcgtgtga tcgagaacac cgtgcgtgaa aaattgccgg aaggcttcca gcgcgccgag
     4741 ttcctggtga ccaagggcgc cgtcgacatg attgtcgatc ggcgcaagat gcgcgaagaa
     4801 atcgcgcatt tgctggcgtt gctgcaaaat caggcggctg aaaccatttc ctgagcgcca
     4861 tgccgcaggc agacctgaac ttgtcgtccc gcgcaagttc gggtttttgt tttcaagtca
     4921 tcgatcaagc ttttccgttt cacgcaggct gatgtcatgc aagcgcaagt ctcccccgtt
     4981 actttggccg actggctggc gcttcttgaa acccgtcacg tcaaggccat cgacatgggc
     5041 ctggagcgtg tcggcaaggt caaggagcgt ctcggcatcc gcttcgactg cccgctcatc
     5101 acggtggctg gcaccaatgg caaaggctcg acctgcgcca tgctggaatc gatcctgata
     5161 cgcgccggct atcgcgtggg cttgtacatc aagccgcatt tcctgtattt caatgagcgt
     5221 gcccgcgtcg cgggtacgcc ggtatcggat gccgcgctga tcgctgtgtt cgaggcggta
     5281 gaagcggtgc gcggcgatat ttcgctgacc tattttgaat tcaccacgct ggcgatcatg
     5341 aagctgctgg ctgatgccag actcgatgtc gttatcctcg aagttggtct gggtggtcgt
     5401 ctggatgcgg tcaacgtgat cgacgccgat gttggcatca tcaccagcat cgacatcgac
     5461 cacaccggat accttggcag cacacgtgaa gaaattggcc ttgagaaggc tggaatttat
     5521 cgcgctggca agaccgccat ctgtggcgat ccgatgccgc caaagtcgct cgtccggtac
     5581 gccgaagcca tcggtgccga tctgtggctg atgggccgtg atttcaatta catgggtgac
     5641 aggctgcaat gggcttatgg tggtcgcgcc cagcgccgca attcactggc ctacccgagc
     5701 ctgcgtggcg ccaatcaatt gctcaatgcc acggcggcat tggccgcgct ggaagcactg
     5761 cgcgatcgcc tgccagtcgg cgcacaggaa gtgcgcgccg gactggcaac ggtggcattg
     5821 ccgggccgct tccaggtttt gccagggcag ccgttggtga ttcttgatgt ggcgcacaac
     5881 ccgcacgccg ccgccacact ggcgcaaaac ctcgataaca tgggatttca tccctatacc
     5941 tacgccgtgt tcggttgcat gctcgacaag gacatcaagg gtatcatcgg gcaactcgag
     6001 ggtaagatcg atcactggtg cgtgactggt ttgccgctgc cgcgcgcggc gacagcgcaa
     6061 caattgaagg ataagttgct ggaagccggc atcgtgccgg aatgcgggtc aaacgctgcc
     6121 tgtacgataa caagctttga tactccagcg gctgcattgg caaatgcaca gagcagagct
     6181 ggcgagaatg atagaattgt agttttcgga tcctttctta ctgttgctgg cgtgatggag
     6241 gctcgtaagc cgaaatgaca tcgagtcgtg ttttgcgttg tggcttatcg attgtcgaca
     6301 ttttaccgat tatctaacct ggatcagcat gggcttgttc tcgtttttcc ataaaaacaa
     6361 gcaggaatct gcttctgatc aaggcgactt gcgttccgca agaaagttgg cggcacgccg
     6421 tcgcagggtt ggcaaagaag atactgccga tcctgcgctg tccgaaaaaa aacgcgcacg
     6481 ccgtcgcctg attggtgtgg ttgcgctggt attggccatg atcatcgttc tgccgatgat
     6541 tctcgactcc gagccgcgtc cgctggccga cgatatcagt attcagatcc cttccagaag
     6601 ttccgtcgcc gatgccaggc cggccgcttt ggccccggca gcggaacagc cgacggcagt
     6661 gcctgtgacc gaaaaaacgg aagtggacgc taaagtcgac accaaagccg acgtaaagga
     6721 aggcagggca gaagcaaaga tggcggacag gcttgccggg catcaagatg atcacagaat
     6781 ggaaaaggat gccggagaaa ccagcaagcc taagactgcc tggaagccgg acgatgacgc
     6841 ccgtgccgta gcgattctgg agggcaggtc tgtagttccc gctggtggtg cgttcgtgat
     6901 tcaggtagct gtgctgacga cccaggaaaa gatcgatgaa ttgcgtaaca agctcagtag
     6961 cgccagtatc aagtcgtata cccaaagggt agcgatccag tcgggtgagg gtgtacgcat
     7021 tcgcgtcggt ccatttgcca atcgtcacga agccgacaag atgcgtgcca ggatcaacaa
     7081 gcttggtctg aatgcgacga tcgttccggt ctgaatggta ctcctgccgg tcgcatatgg
     7141 atcctgaaat tcatttcact gctcatcgcg agtgcgaatg tctctatctg gaaggtggtt
     7201 gctgcgtgcc tcttaccggc acatttttcc ggttgtgacg attttcgatt atctggtgtt
     7261 tttcgtgctg atctgctcgg tgagcatcag catactgcgc ggtctggtca gggaggtgct
     7321 gtcgctggcg agctggacga cggccttcgt tgttgctggc gcctatgctg aggcattggc
     7381 ggtgatgctg cccgacggga ttccgggcgc gtcgacaaaa tggattgtgg gattcattgc
     7441 cttgtttatt ggtgtgcgcc tgttgatggc gctcctgggc agagcggtgc gggaaatggt
     7501 gaaggtgagc ggtctgtcac tgatcgatcg tagtctgggc gcgctatttg gcctggcgcg
     7561 cggcgcggtg attgtgatgg cggtggtgtt gttgtgcggt acgacgtcga tgccgcagca
     7621 gccgttctgg aaagatgcct tgttgagttc gcaggcggtg agcgccgcgc agactgttaa
     7681 gccgtatttg ccgggccagt tcgcgcagca catccatttt taattttgtt tcttgtttag
     7741 gagtccacta tgtgcggcat agtcggcgtc gtttctcata gtccggtcaa ccagttgctg
     7801 tacgatgcgc tgctgctttt gcagcatcgc ggtcaggatg cggctggtat tgccaccaat
     7861 cacgcgaacg gtttttcgat gcacaaagcc aatggtctgg tgcgtgatgt ctttcgcacg
     7921 cgcaacatgc gctctctgcc aggcaacact ggccttggtc aagtgcgcta tccgaccgct
     7981 ggcagctcgt ccagcgaaga agcgcaaccg ttctatgtca atgcaccgtt cggcatcatc
     8041 ctggcgcata acggtaatct gaccaacgcg gaacagctca aggtcgagat gttcaggaac
     8101 gatcgccgcc atctcaatac cgattccgac accgaagtgc tggtcaatgt ttttgcgcat
     8161 gaattgcagc aggcgaccac cagctattca ttggatccgg cagccgtgtt caaggccgtc
     8221 gccatggtcc acaagcgtgt acgcggctcc tatgccgtag tggcgcagat cgccggttac
     8281 ggtttgttgg gttttcgcga tccatacggc attcgtccga tgtgcctggg ttataacgaa
     8341 accgacaagg gtcatgaata catgctggct agcgaatccg tagcgctgga ggggctgggt
     8401 ttccgcttct tgcgcgatgt aatgccgggc gaagcaatct tcatcgatca agacggccag
     8461 ctatacaatc agcaatgcgc cgaaaacccg acactcaatc cctgtgcctt tgaattcgtc
     8521 tatctggcgc gtcccgattc tgtcctcgac ggtgcttccg tctatgccac gcgcctaaag
     8581 atgggcgagt ttctggccga gaaggtccgc cgtgaaattg ccggcggcga gattgacgtg
     8641 gtcatgccga ttcccgattc gtcgcgtcct gccgcaatgg aactggcgct caagctgaac
     8701 ctgcaatatc gcgaaggctt catcaagaat cgctatatcg gccgcacttt catcatgcct
     8761 ggtcagagcc tgcgcaagaa atccgtgcgt cagaaactca atgccatcag cgccgaattc
     8821 aagggcaaga acgtgctgct ggtcgatgac tcgatcgtgc gcggtactac cagccgcgag
     8881 atcgtgcaga tggcgcgcga agccggcgcc aggcgcgtga tctttgcttc ggctgcaccg
     8941 ccagtcaaat ttcccaatgt ctacggcatc gatatgccga cacgcgacga actgatcgcc
     9001 tatggtcgta ccgacgcaga agtctgtcgc gaaatcactg ctgacgcgct ggtgtatcag
     9061 gatgtggaag cgttgaaacg ctcgatctct gatgtcaacc cggaactgaa gaatttcgag
     9121 gcatcgtgtt tcgacggcaa ttatcccacc ggagacatct cgcgtgatta ccttgaccgc
     9181 atcgagttcg tgcgcaaaaa tcccaagcca gcgctggatg acgccatgag ttcacagctc
     9241 aatctaaatc tggcgaacgt ggactgagat agtgcagagt aatcagacga ttttctttcc
     9301 gtcgcgcaat tcacggcgca ggatcttgcc gacattgctt tttggcaatt ctgtacgaaa
     9361 ctcgatatat tttggctgct tgtaaccggt gagccgttcc ctgcagtaaa ccaccagatc
     9421 ctcaatggtc agcgacgcgt ccctgcacac gacaaacagt ttgactgctt caccggtgtg
     9481 ctcgtccggt atgccgacgc aggcgcattc cagcacgcct ggatgggcgg tcgccacttc
     9541 ttcgatttca ttgggataaa cgttgaaacc cgacaccagg atcatgtctt tcttgcgatc
     9601 gacgatacgc gtgtaaccgc gctcgtccat gatgccgatg tcgcctgatt tgaagaagcc
     9661 atccggcgtc atgacttttc tggtttcgtc aggacgattc cagtaagcag ccatgacttg
     9721 cggaccgcgg atcgcaattt cgccaggctg gccggacgct accggttgac cgttgtcgtt
     9781 cacaatccgg atctcggtcg atggcagcgg caaaccgatg ctgccggtgt actcactgat
     9841 agtgtaagga ttggcgctgg cgatcggcga tgtctcggac aagccgtagc cttcgatgat
     9901 ggcgcagccg gtgatctggc gccatttgtc tgctataagc ttttgtactg ccatgccacc
     9961 gccgatg
//
LOCUS       DASRDH010000256         3713 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1102555,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000256 DASRDH010000000
VERSION     DASRDH010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 3713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3713
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1102555"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="VFA14_05965"
     CDS             <1..343
                     /locus_tag="VFA14_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HZS80955.1"
                     /translation="EGSGENVFIIKNGKIYTPDLASCLDGITRDSVLTMARDLGIEVI
                     EKRITRDEVYCCDEAFFTGTAAEITPIRELDNRTIGSGTRGPITEKLQTLFFDIVSGK
                     ASQYQHWLTLV"
     gene            375..569
                     /locus_tag="VFA14_05970"
     CDS             375..569
                     /locus_tag="VFA14_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007878625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HZS80956.1"
                     /translation="MNQAPTQAVELDGEDLPAHCPNPNMPQWSSHPRVFLELNTHGAA
                     RCPYCGTQYQLKPGAIVKAH"
     gene            625..1056
                     /locus_tag="VFA14_05975"
     CDS             625..1056
                     /locus_tag="VFA14_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007878623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HZS80957.1"
                     /translation="MPRAKLLQGSPDEVEAAYYDALARADLTSLMALWAEEEDIICIH
                     PGAVRLVGRAAIRNSWKEILSHGGLRIQSRQLYTAHTMTMAMHNVIEDIQHTEPVQRN
                     VHVIATNTYMKAAHGWCIVARHASIAPGPAPMESFAETMLH"
     gene            complement(1030..2493)
                     /locus_tag="VFA14_05980"
     CDS             complement(1030..2493)
                     /locus_tag="VFA14_05980"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HZS80958.1"
                     /translation="MLPFAVAPHFSAAQTLPALGDTEREGLSPLMERKLGEQIMRDIR
                     CDPDYLDDGPLLEYLNDFGAALVSVYPDVRGEGGYDFFFFAVRDPVLNAFALPGGFIA
                     VHSGLLLAAQSESELASVLAHEIGHVAQRHIARMIGQQKQNSMIQLAAVVLGALAGVS
                     KAGSDVAMATLLGGTGMAEQRQLNFSRDAEREADRIGLQILRDGGFDTSGMVAFFGRL
                     QSASRNFSDVLPPYLQTHPLTTERMADIQARIRDQRYQQHAENPDFQLIRARVRLLQD
                     NSAQGLRDAQVYFESQLRQNSNMQTMASKYGLAYAAYLQRSYDKAENLLQEARSAALQ
                     SMAPSILLDNLDIDIKMAKRHNEQALKAADTLRKQFPISRVAARQYAEALIASRHYDQ
                     AVAYLRDQAQLYRQEVAVQDLLARAYSGQGRQALQHMALAESYVLSGSLPAALDQLGI
                     ARKAPDAGFYEQAIIDARERELKELWQNSSMQHGFGK"
     gene            complement(2843..3232)
                     /gene="rplQ"
                     /locus_tag="VFA14_05985"
     CDS             complement(2843..3232)
                     /gene="rplQ"
                     /locus_tag="VFA14_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008334167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HZS80959.1"
                     /translation="MRHRHGLRKLNRTSSHRLAMLRNMTVSLLRHEAIKTTLPKAKEL
                     RRVVEPILTLGKTDTLANKRLAFSRLRDREIVGKLFSELGPRYAMRNGGYLRILKMGF
                     RVGDNAPMAFVELVDRPEISEAASSAE"
     gene            complement(3361..>3713)
                     /locus_tag="VFA14_05990"
     CDS             complement(3361..>3713)
                     /locus_tag="VFA14_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha
                     C-terminal domain-containing protein"
                     /protein_id="HZS80960.1"
                     /translation="EEAIRQSARVLVDQLSVFAALEGTEVTTEAPSYAPQVDPILLRP
                     VDDLELTVRSGNCLKAENIYYIGDLIQRSETELLKTPNLGRKSLNEIKEVLASRGLTL
                     GMKLENWPPVGLEK"
BASE COUNT          894 a         1124 c          949 g          746 t
ORIGIN      
        1 ggaaggctcc ggcgaaaacg tcttcatcat caaaaacggc aaaatctata cacccgacct
       61 cgcctcctgc ctcgacggca tcacccgcga ttctgtgctg accatggcgc gcgaccttgg
      121 tatcgaagtc atcgagaaac gcatcacccg cgatgaagtg tattgctgcg atgaagcttt
      181 cttcaccgga acggcagcag aaatcacacc gatccgcgaa ctcgacaacc gcaccatcgg
      241 tagcggtacg cgtggcccaa tcactgaaaa actgcaaacc ctgttcttcg acatcgtttc
      301 aggaaaagca tcgcaatatc agcattggct gacgctggtt taatagcaaa tgagaacgtg
      361 tcacggagaa tcccatgaac caggcgccaa cccaggcagt agaactcgac ggcgaagacc
      421 tgccggcgca ttgccccaac ccgaatatgc cacaatggtc gtcgcacccg cgcgtctttc
      481 tggaactgaa tacgcacggc gccgccaggt gcccctactg cggcacgcaa tatcagctca
      541 aacccggcgc tatcgtcaaa gcgcactgaa aaagaggacc ggcagacatg actgaccctc
      601 tttactttta cttctggaga aaacatgcca cgcgccaaac tgctgcaagg ctcccccgat
      661 gaagtcgaag cggcctacta cgatgcgctt gccagagccg atctgacatc cttgatggcg
      721 ctctgggccg aagaggaaga catcatctgc attcatcccg gtgcagtgcg cctggtcggc
      781 cgtgcggcaa tccgcaactc ctggaaagaa atcctctccc atggcggcct gcgcattcag
      841 tcacgccaac tatataccgc ccataccatg accatggcca tgcacaacgt catcgaagac
      901 atacagcata cagaacctgt tcagcgcaat gtacatgtga tcgccaccaa cacctatatg
      961 aaggccgcgc atggctggtg catcgttgcc cgccatgcct ccatcgcccc cggcccggcg
     1021 ccgatggaat catttgccga aaccatgctg cattgaactg ttctgccaca gttctttgag
     1081 ttcgcgttcg cgtgcatcga tgatggcctg ctcatagaaa cccgcatcag gcgccttgcg
     1141 agcaatgccg agctggtcga gtgctgccgg cagactgccg gacaacacat aggattccgc
     1201 caatgccata tgttgcaacg cctgcctgcc ctgccccgaa taggccctag caagcaaatc
     1261 ctgcaccgcg acttcctgcc ggtacagctg tgcctgatca cgcagatacg ccaccgcctg
     1321 atcataatgc cggcttgcaa tcaaggcttc ggcatactgg cgcgcagcga cccgcgaaat
     1381 cgggaactgc ttgcgtaagg tatccgccgc tttcagcgcc tgctcattat gccgtttggc
     1441 catcttgata tcaatgtcga gattgtcgag cagaatactc ggcgccatcg attgcagcgc
     1501 tgccgagcgc gcctcttgca acagattttc tgccttgtca taagagcgtt gcagatatgc
     1561 cgcataggcc aatccatact tggatgccat cgtttgcatg ttgctgttct ggcgcaattg
     1621 cgattcaaaa tagacttgcg catcgcgcag gccctgtgcc gaattatctt gcaacaagcg
     1681 cacgcgggca cggatcaact gaaagtcagg attctcggcg tgctgctggt aacgctgatc
     1741 gcggatacgc gcctggatgt cagccatccg ctcggtagtc aacggatgcg tctgcagata
     1801 cggcggcaag acatcgctaa aattgcgcga tgcactttgc aggcgaccga aaaacgcgac
     1861 catgcccgag gtatcaaaac caccgtcgcg caaaatctgc aagccgatgc ggtcggcttc
     1921 acgttcagca tcgcgactga agttcaactg ccgctgctca gccatgccgg tgccgcccag
     1981 cagagtcgcc atggcaacgt cgctaccggc tttcgacacg cctgccaggg cacccagcac
     2041 aacggccgcc agttgaatca tggaattctg cttctgctgg ccgatcatgc gcgcgatatg
     2101 acgctgcgcg acgtgaccga tttcatgcgc cagcacagag gccaattcag attcactttg
     2161 cgccgccagc aacaagcctg aatgcactgc aataaaaccg cccggcagcg caaatgcatt
     2221 gagcaccgga tcgcgtaccg caaagaaaaa gaaatcgtag ccgccttcgc cgcgcacgtc
     2281 gggatacacc gataccagag cggcgccaaa gtcgtttaga tattccagca atgggccgtc
     2341 atcaagataa tccggatcac agcgaatatc gcgcatgatc tgctcgccca gcttgcgctc
     2401 catcagtggc gacaacccct cacgttccgt atcgccgagc gcgggcaatg tttgcgcggc
     2461 actgaaatga ggcgcgacag caaacggcaa cacgacaaat gacaaagcca acgccgctga
     2521 caaggagcgc caacgcggcc acagtgacgt aacgacagat gatttttggg aagaaacgac
     2581 ggactgacgc ataaagacga tgaaagaagt atctgcgatg cctgtatgat acgcgcagta
     2641 ctccctcgcc gcttccggca tcccgtcgac gacagctgga acgatagagc gtgttaggcg
     2701 ctattttaat ccgagcggcc agacactcat atggcagacg tgaatggcaa cacgtaaaac
     2761 ggcccggggc gcaagcgcga ccgggccgcc gagtcctgat ttttccgcta caccttcgat
     2821 acctggtcaa caggccgcaa gtctattcag cgctcgatgc tgcttcggag atttctggac
     2881 gatcgaccaa ttcaacaaaa gccatcggcg cattgtcgcc gacgcgaaag cccattttca
     2941 gaatacgtag atagccaccg ttacgcatcg cgtaacgtgg tcccagttcg gaaaacagct
     3001 tgccgacaat ttcacgatca cgcagacgcg agaacgccag acgcttgtta gccaatgtgt
     3061 cagtcttgcc tagggtcagg ataggctcaa caacgcggcg cagttccttg gcttttggca
     3121 aagtggtttt gatggcttcg tgacgcagca gagaaactgt catgttgcgc aacatcgcca
     3181 ggcgatgtga ggaagtacga ttcaatttgc gaagaccatg acggtgacgc atgatatttc
     3241 ctttcagatt acagttttca tccagctctt ctatcggcct gggcgccgcg gaccggtatc
     3301 gattgggaga tcaggggcag gctgctgaac ccggcactgc ccgccagcac attgcattga
     3361 ttacttttcc agaccgaccg gcggccagtt ttccagcttc atacccaaag tcaaaccacg
     3421 ggatgccagc acttccttga tttcattcag agacttacgg cccagattcg gcgtcttcaa
     3481 cagctcagtc tcactacgtt ggatcagatc gccaatgtaa tagatgtttt ctgctttcaa
     3541 gcagtttcct gaacggacgg tcaactccag atcatcgacc ggacgcagca ggataggatc
     3601 gacttgcggt gcatacgacg gcgcttcagt agtcacttcc gtgccttcca gcgcggcgaa
     3661 tacactaagt tgatccacca gaacccgcgc cgattgacga atcgcttctt ccg
//
LOCUS       DASRDH010000257         2162 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1559336,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000257 DASRDH010000000
VERSION     DASRDH010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1559336"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="VFA14_05995"
     CDS             <1..310
                     /locus_tag="VFA14_05995"
                     /inference="COORDINATES: protein motif:HMM:NF015935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HZS80961.1"
                     /translation="ILYIELVSLGSVKSTIVEPMNVYRFAVMKNAVRVIAVHNHPSGN
                     LTPSPHDKDTTDRLIQVGRNQKNALIKFLMEKNTATEEIANILKIPPEEVEKATKKKT
                     "
     gene            complement(424..1344)
                     /locus_tag="VFA14_06000"
     CDS             complement(424..1344)
                     /locus_tag="VFA14_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016833737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HZS80962.1"
                     /translation="MLVSLTRLFPLWALLLSVAAYFSPSTVTPIGAYVTPLLMLIMLT
                     MGVTLTLDDFKRIVKRPAPVAAGIVLHYLIMPLAAWLIARSLRMPPDLTAGMVLVGSV
                     ASGTASNVIIYLSRGDVALSVTISTLSALVSVFATPLLTELYVDTSIHFDTRAMLMSI
                     VQIVVAPIAVGLALNLFAGKLIRRIEPCLPLISMVAILLIIAAVVGGSQASIASVGLV
                     VLVGVILHNALGLLGGYWGGRLLGFDEAVCRTLAIEVGMQNSGLAAALGKLYFTPLAA
                     LPGALFSVWHNLSGSMLASIWRDRPPKERT"
     gene            complement(1396..>2162)
                     /locus_tag="VFA14_06005"
     CDS             complement(1396..>2162)
                     /locus_tag="VFA14_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004186903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZS80963.1"
                     /translation="YQKRNETQEIFIPVAERLGNEVIEFKNVSKGYGDRLLIDNLSLK
                     IPAGAIVGIIGPNGAGKSTLFRMLAGREQPDSGELALGPTVSISLVDQSREDLENRKT
                     VFEDLANGADILTVGRFEMPSRAYLGRFNFKGGDQQKIVGKLSGGERGRLHLAKTLLQ
                     GGNVLLLDEPSNDLDVETLRALEDALLEFAGTVLVISHDRWFLDRIATHIIAFEGDSQ
                     VTFFDGNYQEYEADKKKRLGEEGAKPKRIRYRPVTR"
BASE COUNT          504 a          633 c          634 g          391 t
ORIGIN      
        1 catcctctat atcgaactgg tcagcctggg cagtgtgaaa tccaccatcg ttgaaccgat
       61 gaatgtctat cggtttgcag taatgaaaaa cgccgtccgg gtgattgcag tacacaatca
      121 tcctagcggc aacctcactc cctcgcccca tgacaaggac acgacagatc gcctgatcca
      181 ggtcggccgc aatcagaaaa acgccttgat caaattcctg atggaaaaaa atacggcgac
      241 agaagagatt gccaacatac tgaaaattcc gccagaggaa gtcgaaaaag cgaccaaaaa
      301 gaaaacatag tcccttgcgc gacctgcttt gggtcaagac gtactgaccg agtagccatc
      361 ggcaggccgc agccccattc cgcaacggtg atgggactac cgtcccgcgc cgggcaatca
      421 atatcaggtc cgctccttcg gcggacggtc gcgccagata ctggccagca tcgatcccga
      481 cagattgtgc cagaccgaga acagcgcgcc cggcaatgcc gccagtggcg tgaagtacag
      541 tttgcccagc gcggcggcaa gaccggagtt ctgcatgccg acttcgattg ccaacgtgcg
      601 gcaaactgct tcatcgaaac cgagcaggcg gccgccccag taaccgccca gcaggcccag
      661 cgcattgtgc agaatgacgc cgaccagcac caccaggcca accgacgcga tgctcgcctg
      721 actgccgccg acgacggcag cgatgatcag cagaattgcc accatcgata tcagtggcag
      781 acagggttcg atgcggcgga tcagtttgcc agcaaacagg ttcagcgcca ggccgacggc
      841 aatgggcgcc actacgatct gcacgatgct catcagcatc gcgcgcgtat cgaagtgaat
      901 cgaagtatcg acatacagct cggtcagcag cggcgtagcg aacacgctga ccagcgccga
      961 cagtgtcgaa atcgtcaccg acagggcaac gtcgccacgc gacaggtaaa tgatcacgtt
     1021 agaggccgta ccgctggcga cgctgccgac cagcaccatg ccggcggtca ggtcgggcgg
     1081 catgcgcagc gacctggcga taagccaggc ggccagcggc atgatgaggt agtgcaggac
     1141 gatgccagca gcgaccggcg ccggacgttt gacgatgcgt ttgaaatcat ccagcgtcag
     1201 ggtcacgccc atggtcagca tgattagcat cagcaggggc gtgacgtacg ctccgatcgg
     1261 cgtgacagtg gatggcgaaa aataagcagc aacggacagc aacagcgccc acagcgggaa
     1321 cagacgggta agactgacaa gcatggtaaa acgatcgttt aaacggagtg ggagaaatgg
     1381 tggccttgcc gacagtcagc gcgtaacagg ccggtaacgg atccgtttcg gctttgcgcc
     1441 ttcttcaccc aggcgtttct ttttgtcggc ttcgtattcc tgataattgc cgtcgaaaaa
     1501 agtgacctgc gaatcgccct cgaacgcgat gatgtgggtg gcgatacgat cgaggaacca
     1561 gcgatcgtgg ctgatcacca gtacggtgcc agcgaattcc agcaaggcgt cttccagcgc
     1621 gcgcagggtc tccacgtcga ggtcgttgga cggttcatcg agcagcaaga cgttgccgcc
     1681 ttgcagcaac gtcttggcca gatgcagacg gccgcgttcg ccgccggaca gcttaccgac
     1741 gattttttgc tggtcaccgc ccttgaagtt gaaacggccc agataggcgc gcgacggcat
     1801 ttcgaaacgg cccacggtga ggatgtcggc gccattggca aggtcttcga acacggtctt
     1861 cctgttctcc agatcttcgc gcgactgatc gaccagcgag atgctgacgg tgggtccgag
     1921 cgccagttcg ccgctgtccg gctgttcgcg gcccgccagc atgcgaaaca gggtcgattt
     1981 gccagcgccg ttggggccga tgataccgac gatggcgccg gccggaatct tgaggctgag
     2041 attatcgatc agcagacgat cgccataccc cttgctgacg tttttgaatt cgatgacttc
     2101 attgcccaga cgctcggcca cgggaatgaa aatttcctgg gtttcgttac gcttctggta
     2161 tt
//
LOCUS       DASRDH010000258        10336 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_872613,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000258 DASRDH010000000
VERSION     DASRDH010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 10336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10336
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_872613"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1061
                     /locus_tag="VFA14_06010"
     CDS             <1..1061
                     /locus_tag="VFA14_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZS80964.1"
                     /translation="QIPFGIASDRYGRKRIIVLGLVLFASGSFVAASAHDIVWVIAGR
                     AIQGAGAISAAVTALIADATREEHRTKAMAMVGASIGLSFAVSLVASPLLYELIGIGG
                     IFSLTGMLALAAIAVVLFVVPDAPMVEARHVPFDAVLRDVELMRLNFGVFVLHMTQMA
                     MFVVLPAALVQYAGLPVAEHWKIYLPVVLLSFVFMLPPVFIGEKRGMMKQVFVAAISL
                     LLVVQLGMWLLTLSHSAVRWWMPAGLLLAFFIAFNILEACQPSLVSRIAPAAAKGAAL
                     GVYNTLQAFGLFCGGALGGWLKQHLSASSVFVMCAVAILIWLIIAFNMKNLPRRGIQT
                     AGATAQPVTFRRNNGLGQ"
     gene            1045..1542
                     /gene="ssb"
                     /locus_tag="VFA14_06015"
     CDS             1045..1542
                     /gene="ssb"
                     /locus_tag="VFA14_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008332119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HZS80965.1"
                     /translation="MASVNKAIIVGNLGRDPETRYMPNGEAVTNITVATTESWKDKNS
                     GEKKELTEWHRITFYRKLAEIAGQYLKKGSQVYVEGRLQTRKWTDKDGIDKYTTEIIA
                     DAMQMLGSRQGMGGSAPSIDEGGGYGNAPARQGSGTSDDGAARTPAAARPVSNFSDMD
                     DDIPF"
     gene            complement(1613..2431)
                     /locus_tag="VFA14_06020"
     CDS             complement(1613..2431)
                     /locus_tag="VFA14_06020"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HZS80966.1"
                     /translation="MYIDSHCHLNFPELAARLPDIFGKMTENRVSHALCVSVNLPEFP
                     QVLALAEQYPRIYASVGVHPDYQDTPEPTVEELVALANHPRIVAIGETGLDYHRLEGD
                     LEWQRTRFRTHIRASRITGKPLIIHTRCASEDTIRLMRDEGADPAAGGAAGVMHCFTE
                     SQEVADAALALGFYISFSGIVTFKSAKALQEVARTIPLERMLIETDSPYLAPAPYRGK
                     TNEPGLVRHVAEFIADLRGISVEEVACHTTGNFFTLFKVAGPDNETREEVPGTC"
     gene            complement(2478..2984)
                     /locus_tag="VFA14_06025"
     CDS             complement(2478..2984)
                     /locus_tag="VFA14_06025"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZS80967.1"
                     /translation="MNNIAYTHRPARQEDLGVIVAIYNSTIASREVTADTEPVSVASR
                     QAWFDEHTPDRRPLWVAECHGEVLGWLSCSSFYGRPAYSGTVEVSVYIHEKARRQGLG
                     RYFLEQVIASAPTIKVHTLLGFVFGHNRASLKLFEAFAFERWATMPRVATLDSVERDL
                     VIVGKRVA"
     gene            complement(2993..4021)
                     /locus_tag="VFA14_06030"
     CDS             complement(2993..4021)
                     /locus_tag="VFA14_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007877607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HZS80968.1"
                     /translation="MNASVLPWLRESWLQLQQLRACLPHAILFYGPEGIGKTGFVEMF
                     AQSLLCENAQADGHACGACASCGWFSQYNHPDYRRVRPEILDGGEAAEDGDSEEKKTA
                     RAGKVFSKEIRIDQIRALADFMNVSTHRSGMRVVVLYPAEALNAAAANALLKTLEEPP
                     PNTMFLLIGNRLDRLLPTVLSRCSKFALALPSRAASLAWLRQQNIEDADVWLAEQGGA
                     PLAALDASQSDSRAVMDEFLRALSHPGIDIALKTAERLQKIPLVDLVGWQQRWLYDVF
                     ALQSGGAIRYYPRYSKELGALNGRIEAAAIAAALKAANARRAIADHPLSARLFIEEML
                     LEYATLFA"
     gene            complement(4018..4635)
                     /gene="tmk"
                     /locus_tag="VFA14_06035"
     CDS             complement(4018..4635)
                     /gene="tmk"
                     /locus_tag="VFA14_06035"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020703240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HZS80969.1"
                     /translation="MTSGKFITLEGIDGAGKSTHLAFIADLLRARGLSVVTTREPGGT
                     ELGESLRAMLLHQKMHLETEALLMFASRREHLAQLIEPALARGDWVISDRFTDATFAY
                     QGGGRHLALDKLDALEQWVHPHLQPDLTLLFDVPLEVARARLGAERALDRFEQEQADF
                     FAAARAGYLCRAQQFPQRFCVIDSTRSIGQIQQEIAAILDKIDYL"
     gene            complement(4648..5628)
                     /gene="mltG"
                     /locus_tag="VFA14_06040"
     CDS             complement(4648..5628)
                     /gene="mltG"
                     /locus_tag="VFA14_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013234576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HZS80970.1"
                     /translation="MKKLVDALLLLCVACACAAVYWMSLPIIGEGGQPVEFTIAPGAG
                     IYGAARQIASAGAPLDPLLFTLLARGSGKGRHIKAGSYELEPGATPLSLLEQLVRGEL
                     AQESLTIIEGWSFRQMRQAIAEHQGLKHDTTHLSDSELLTQLTDEFTVPEGLFFPDTY
                     LFAKGASDMQIYRQAFTLMNKRLSEAWSRRDPSLPYRTPYGALIMASIVEKETGQKSE
                     RGMVAGVFVNRLKAGMLLQTDPTVIYGMGGRYQGVIRKSDLLTDTPYNTYTRKGMPPT
                     PISLPGAASLAAAMNPDKTDALYFVARGDGTSHFSSNLDAHNQAVNQYQR"
     gene            5725..6801
                     /locus_tag="VFA14_06045"
     CDS             5725..6801
                     /locus_tag="VFA14_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007877596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HZS80971.1"
                     /translation="MIESTLALSPWQAFLVQQGARIEAENDAEVRHFGACTTALPENF
                     VAPLTDLGLIALSGDDAAGFLHRQITNDVEKLDPANVRLAGYCTPKGRLLATFLMWKE
                     SGQIMLQLPRTILPPVQKRLRMFVLRARVQLTDVSEQYVTLGLAGDAAAQVLQQWFPH
                     LPAHAYTRIANDNGTLMRLADSYRTPRYQWITNTGQAISAWPVLTQKLTATGTYAWRL
                     SDIYAGVPMITQPTQERFVPQMINFELIGGVDFKKGCYPGQEIVARSQYLGKLKRRSV
                     LALIDATGVHAGAEVFSSVDAGQPCGMVINAEPAGNGQSACLVEIKLAAADNGSIHLG
                     AADGPVLQLQKLPYTFFAQETRNK"
     gene            7034..8272
                     /locus_tag="VFA14_06050"
     CDS             7034..8272
                     /locus_tag="VFA14_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZS80972.1"
                     /translation="MTPAPVAATPPPGFSRYQKFVVGMLAFLQFAVILDFMLMSPLGA
                     LIMPAMQIMPRQFGLVVSAYAFSAGISGLLTAGFADRFDRKKLLLFFYTGFIIGTLWC
                     GLAQSFESLLAARVVTGLFGGVIGSIVLAISTDLFPLQMRGRVIGIIQTAFAASQVLG
                     IPISLYLSNRWNWHIPFIAMAAFSIIGGVIIMLKMQAVADHLNIPQEHSAWRHLFHTV
                     TEPRYLTAFATTALLTTGGFMLMPFGSAYLVSNLGIDIQHLPTVYLIAGLCTIGIGPL
                     IGTASDKFGKFRIFLFGTLLSISIVLIYTHLGRTAMPLVIVVNVVMLLGIFSRMIPFQ
                     ALVSSVPMVNQRGSFNAVSASIQQLSGGLASVAAGRIISLGADGKLQNFDIVGYVFIG
                     TSLMALALVWRLSRHLENHT"
     gene            complement(8378..8454)
                     /locus_tag="VFA14_06055"
     tRNA            complement(8378..8454)
                     /locus_tag="VFA14_06055"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8418..8420),aa:Val,seq:gac)
     gene            complement(8539..>10336)
                     /locus_tag="VFA14_06060"
     CDS             complement(8539..>10336)
                     /locus_tag="VFA14_06060"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008328026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HZS80973.1"
                     /translation="RVVLLRLAARVTTLRHFAENRLENDITTQYARATFDLYAPLANR
                     LGIWPLKWELEDLSFRFLEPATYRRIARMLEEKRMERTEFVARAIEHLRTELTAAEIK
                     AEVSGRPKHIYSIWNKMRAKALAFSDLYDVRAFRVITDDVKDCYTVLGIVHDIWTPVP
                     EEFDDYIARPKQNAYQSLHTVVIAEDGRPLEVQIRTHEMHHFAEYGMAAHWRYKESGG
                     SSFSAQKYDEKIAWLRQLLAWKSEVAGAVVEQEEARRDWVAKLKSTTLDDRIYVLTPQ
                     ARVIELPNGATPIDFAYCLHSDIGHCCRGARVDGIMVPLNTPLKNGQTIDIITVRNDP
                     GAGPSRDWLSPGYVVSSRARAKLRAWFNAVKQQETLAHGRTILEKTLQREGRTAINLE
                     ELAHRLGFAKVDDLCLALGKDAFSSRQIEHILRAGEAVKQVEEACITRKSRASSVMQG
                     VRPGVLVVGTEGLMTQLARCCKPVPPDSIAGFVTRGKGVSIHRSNCKNLAEMHSKAPE
                     RIIQTTWGTPAGDTVYPVDIFVLAGERQGLLRDISDIFLREKVNVIGVRIRSAKGQAR
                     MSFTAEIGSTAPLQKALAVIREVEDVLEAGRQ"
BASE COUNT         2195 a         3093 c         2849 g         2199 t
ORIGIN      
        1 gccagattcc gtttggcatc gcttccgaca gatacggtcg caagcgcatc atcgtgctcg
       61 gactcgtgct gttcgcatca ggctctttcg tcgcggccag cgcccatgac attgtctggg
      121 tgattgccgg acgcgcgatt cagggcgccg gggcgatttc cgctgcggtg acggcattga
      181 tcgccgacgc aacgcgagaa gaacaccgca ccaaagccat ggccatggtc ggtgcatcga
      241 tcggcctgag cttcgccgtt tcgctggtag catcgccgtt gctgtacgaa ctgattggca
      301 taggcgggat tttttcgctg actggcatgc tggcactggc ggcgattgcc gtagtgctgt
      361 tcgtggtgcc ggatgcgccc atggtcgaag cccggcacgt accgttcgat gcagtactgc
      421 gcgatgttga actaatgcgt ttgaactttg gcgtattcgt cttgcacatg acgcagatgg
      481 ctatgttcgt ggtgctgccg gcagcgctgg tgcaatatgc cggcctgccg gtcgccgagc
      541 actggaaaat ctatctgccg gtcgtgttgc tgtctttcgt gttcatgctg ccgccagtgt
      601 tcatcggcga aaagcgcggc atgatgaaac aagtcttcgt tgccgcgatc tcattgctgc
      661 tggtcgtaca actcggtatg tggctgctca ccctgtcgca ttccgccgtc cgctggtgga
      721 tgccggctgg cttgctgctg gcttttttca ttgccttcaa catcctcgaa gcatgccagc
      781 catcactggt atcgcgcatc gcgccggcgg cagccaaagg cgcagcgctg ggcgtctaca
      841 acaccttgca ggccttcggt ctgttctgtg gcggcgcttt gggcggctgg ctcaaacagc
      901 atctcagcgc atcatccgtg ttcgtaatgt gcgcggtggc gatactgatc tggcttataa
      961 tcgcattcaa catgaaaaat ctgccgcgcc gcggtataca gacggcaggc gcaactgctc
     1021 aaccagtgac attcaggaga aacaatggcc tcggtcaata aagccatcat cgtcggcaac
     1081 cttgggcgtg acccggaaac gcgctacatg cccaacggcg aagccgtcac taacattacc
     1141 gtcgctacga ctgaaagctg gaaagacaag aacagcggcg agaagaaaga actcactgaa
     1201 tggcatcgca tcacctttta tcgcaaactg gccgagattg ccggccagta tctgaagaaa
     1261 ggttcgcagg tctacgtaga aggccgtctg caaacacgta aatggaccga caaagacggc
     1321 attgacaagt acactaccga aatcatcgcc gacgccatgc aaatgctcgg cagtcgtcaa
     1381 ggcatgggcg gcagcgcgcc atcgatcgat gaaggcggcg gctacggcaa cgctcctgcg
     1441 cgccagggca gcgggacaag tgatgatggc gcggcgcgga caccggccgc ggcacgtcca
     1501 gtgtcgaatt tctccgacat ggatgacgat atcccgtttt gagagaatca cctcctgcat
     1561 agcgcgacaa tgcgcgagta tggacaacca gacgcactct ccaattaagt tcctaacacg
     1621 ttccaggaac ctcctcccgg gtttcgttat ccggcccggc taccttgaac agggtgaaaa
     1681 aattcccggt ggtatgacac gcaacttcct ccaccgaaat cccgcgcaaa tcagcgatga
     1741 actccgccac atgccgcact aaccccggtt cgttggtctt gccgcgatat ggcgccggcg
     1801 ccagataggg cgaatcggtt tcgatcagca tgcgctccag cggaatggta cgcgctacct
     1861 cctgcaacgc cttggcgctc ttgaaggtga cgatgcccga gaaggaaata taaaaaccga
     1921 gcgcgagcgc ggcgtcggcg acttcctgcg attccgtaaa gcaatgcatg acgccagcag
     1981 cgcctccggc tgcgggatcg gcaccttcgt cacgcatgag gcgaatggtg tcttccgatg
     2041 cgcagcgggt atggatgatc agcggcttgc cggtgatgcg cgaagcgcgg atgtgcgtgc
     2101 gaaaacgcgt gcgctgccac tccagatcac cttccagacg atgataatcc aggccggttt
     2161 cgccgatggc gacgatgcgc ggatgattgg ccaatgcgac cagctcctcg accgtcggtt
     2221 ccggcgtatc ctggtaatca gggtgtacgc cgaccgaggc atagatgcgc ggatattgct
     2281 ccgccagcgc cagcacctgt ggaaactcgg gaagattcac cgacacacac aacgcatggc
     2341 tgacacggtt ttcggtcatc ttgccgaaaa tgtccggcag acgtgcagcg agctcgggaa
     2401 aattgagatg gcaatgggaa tcgatataca tgatgggcac tattttacgt catcacccga
     2461 ctacagcctg cgggtaatca cgccacccgt ttccccacga tcaccagatc acgctccacg
     2521 ctgtcgagtg tcgccacacg cggcatagtc gcccagcgct cgaaagcgaa tgcttcgaac
     2581 agcttcaggc tcgctcgatt gtggccaaac acaaagccca gcaaggtatg caccttgatc
     2641 gtaggggcgc tagcaatcac ctgttcaaga aaataacggc ccagcccctg acggcgcgcc
     2701 ttttcatgaa tatagacgga cacctcgacg gtacccgaat acgccgggcg gccataaaaa
     2761 ctggagcagg atagccagcc cagcacctcg ccgtgacact cggcgaccca cagcggccgc
     2821 ctgtcgggcg tatgttcgtc gaaccaggct tgacgcgaag cgaccgatac cggctcggta
     2881 tcggcggtga cttcgcgcga ggcgatggta ctgttgtaga tggcgacgat gacgcccagg
     2941 tcttcctgac gcgccgggcg atgagtgtag gcaatgttgt tcatgcaagg tatcaggcaa
     3001 acagtgtggc atattcgagc agcatttcct cgatgaacag gcgcgccgac aaaggatgat
     3061 cggcgatggc gcggcgcgca ttggcagctt tcaatgccgc tgcgatcgcc gcggcttcga
     3121 tgcgcccgtt caatgcgccc agttccttgc tgtagcgtgg ataatagcgg attgctccgc
     3181 cggattgcag cgcgaacaca tcatacagcc agcgctgctg ccagccaacc agatcgacga
     3241 gcgggatttt ttgcaggcgt tcagcggtct tcagcgcgat atcgatgccg ggatggctca
     3301 acgcgcgcag gaactcgtcc ataaccgcac ggctatcgct ttgcgacgca tcgagcgccg
     3361 ccaacggcgc gccgccctgc tctgccagcc agacgtcggc atcctcaatg ttttgctgcc
     3421 tgagccaggc cagactggca gcgcgcgacg gtagcgccag cgcgaacttg ctgcagcgcg
     3481 agagcacggt cggcagcagg cgatcaagcc ggttgccgat cagcagaaac atggtgttcg
     3541 gcggcggctc ttccagcgtt ttgagcaagg cattggcagc ggcggcgttc aaggcttcag
     3601 ccggatacag cactaccacg cgcatgccgg aacgatgcgt cgacacgttc atgaaatcag
     3661 ccaatgcacg gatctggtcg atcctgattt cctttgagaa caccttgccg gccctggcgg
     3721 tttttttttc ttcgctgtcg ccatcctctg cggcctcgcc accgtcgaga atttccggcc
     3781 gcacgcggcg atagtccgga tgattgtatt gcgaaaacca gccacaggaa gcgcaggcgc
     3841 cgcaggcatg accgtcggcc tgcgcgtttt cgcacaacag cgactgcgca aacatctcta
     3901 cgaaaccggt cttgccaatg ccctccgggc cgtaaaacag aatggcgtgc ggcagacacg
     3961 cgcgcaactg ctgcaattgc agccagctct ctcgcagcca gggaagaacg ctagcattca
     4021 tagataatcg atcttgtcaa gaatcgccgc gatctcctgc tgaatctgcc caatggagcg
     4081 ggtcgaatca atcacgcaaa aacgctgcgg aaactgctgc gcgcggcaca aatacccggc
     4141 gcgtgcggcg gcaaaaaaat cggcctgctc ctgctcgaac ctgtccagcg cgcgttccgc
     4201 gcccagacgc gcgcgcgcta cttccagcgg cacatcgaac agcaacgtga ggtccggttg
     4261 cagatgggga tgcacccatt gttccagcgc gtcgagttta tctagtgcca aatgacgacc
     4321 accgccctga taagcgaagg tcgcatcagt gaaacggtcg gaaatcaccc agtcgccgcg
     4381 cgccagcgcc ggctcgatga gctgcgccag atgctcccga cgactcgcga acatcagcag
     4441 cgcttcagtt tctaggtgca tcttttgatg cagcagcatg gcgcgcaggc tttcgcccag
     4501 ctccgtccct cctggttcgc gcgtcgtcac cacgctcaga ccgcgcgcgc gcagcagatc
     4561 tgcgataaaa gcgaggtggg tcgacttgcc agcgccgtca atgccttcaa gagtaatgaa
     4621 tttgcctgat gtcatatgag gtatacgcta gcgctggtac tggttgactg cctgattatg
     4681 cgcgtccaga ttgctggaaa aatgactggt gccgtcaccg cgcgccacga aatacagagc
     4741 gtcagtcttg tccggattca tcgcggcagc cagcgaggcc gcccccggca gggaaatcgg
     4801 cgtcggtggc atacccttgc gggtataggt attgtacggc gtatcggtca acaggtcgct
     4861 tttacggatc acgccctggt aacgaccgcc catgccatag atgacggtcg gatcggtttg
     4921 cagcagcatg ccagctttca gacgattgac gaaaacgcca gcgaccatgc cgcgctcgga
     4981 cttctgaccg gtttcctttt caacgatcga ggccatgatc agcgcaccgt aaggcgtcct
     5041 gtatggcagt gacggatcgc gcctgctcca agcttcgctc aggcgcttgt tcatcaaggt
     5101 aaaagcctga cggtaaatct gcatgtcgct ggcgcctttg gcaaatagat aggtatccgg
     5161 aaaaaataaa ccttccggca ccgtgaactc atcggttagc tgggtcagta attcgctgtc
     5221 agacaaatgc gtcgtgtcat gcttgagtcc ctgatgctcg gcaatggcct ggcgcatctg
     5281 cctaaaactc cagccttcga tgatggtcag cgattcctgg gcgagctcgc cgcgcaccag
     5341 ttgctctaac aagctcaacg gcgtggcgcc cggttccagt tcatagctac cggccttgat
     5401 gtgcctgcct ttgccgctgc cacgcgccag caaggtaaac aacagcggat cgagcggcgc
     5461 gccggcgctg gcgatctgcc gggcggcgcc gtatatgccg gcgccgggag caatggtgaa
     5521 ctcgaccggc tgcccacctt ctccgataat cggcaggctc atccagtaaa ctgccgcgca
     5581 ggcacaggca acacataaca gcagaagagc gtcaactaac tttttcatcg tgaaacaaaa
     5641 ccggacacaa tccgacgcgt ttttcccgcg ccactataat caaggcatca ataataatcg
     5701 ctgtatgact ccaaaagcca ttttatgatc gaatcgacac ttgcgctttc accctggcag
     5761 gcattcctgg tacagcaagg cgcgcgcatc gaagctgaaa acgacgctga ggtacgccat
     5821 ttcggcgctt gcacaacggc actgccggaa aatttcgttg cacccctgac cgatctcggc
     5881 ctgattgcct tgagcggcga cgatgccgcc ggtttcctac acaggcaaat caccaacgac
     5941 gttgaaaaac tcgacccggc gaatgttcgc ctagccggct actgcacgcc caagggtcgc
     6001 ctgctggcga ctttcctgat gtggaaagaa agcggacaga tcatgctaca gttaccgcgc
     6061 acgatcctgc caccggttca gaaacgtttg cgcatgttcg tgctgcgcgc cagggtccag
     6121 ttgaccgacg tcagcgaaca gtatgtcacg ctcgggctgg ccggcgatgc tgcggcacag
     6181 gtcttgcagc aatggttccc gcacttgccc gcgcacgcgt ataccaggat agccaatgac
     6241 aacggtacgc tgatgcgcct tgccgacagc taccggacgc cgcgctatca atggattaca
     6301 aacaccggac aggcaatctc cgcgtggccg gtactgacgc aaaaactcac ggccaccggc
     6361 acatacgcct ggcgcctgag cgacatctat gccggcgtgc cgatgattac ccagccgaca
     6421 caggaacgat tcgtcccaca gatgatcaat ttcgagctga tcggcggtgt cgatttcaaa
     6481 aaaggctgtt atcccggtca ggaaatcgtt gcgcgcagtc aatatctcgg caaactcaag
     6541 cgccgcagcg tactggcgct gatcgatgcg accggcgtgc atgccggtgc tgaagtattt
     6601 tccagcgtgg atgcaggcca gccctgcggc atggtcatca atgccgaacc cgccggcaac
     6661 ggccagtcgg cttgcctggt cgagatcaaa ctggccgccg ccgacaatgg cagtattcat
     6721 ctgggcgctg ccgatggtcc tgtcctgcaa ttgcagaagc tgccttatac atttttcgcg
     6781 caagagacaa gaaataaata gaacaaatat ctggtatttt cgaaataaag tagcctgatt
     6841 tgatgcagtg cattagaatc agcactgtct cctccaattt ttcctaaaaa ttggatttaa
     6901 gcccacaacc ttgcggttgt gggctttttt tatgccgctt tcagttcatc ctggaaatgt
     6961 ttcatttctt ccacgcaggt tacaatatcg cgcttaaaat gaggccgact tctgtcgccc
     7021 cagacaagac aacatgactc ccgctcccgt cgctgcgacg cctccccctg gattttcccg
     7081 ctatcagaaa ttcgtcgttg gcatgctcgc ttttttgcag tttgccgtca ttctcgattt
     7141 catgctgatg tcgccactgg gcgcgctgat catgcctgct atgcagatca tgccgcgtca
     7201 gttcggcctg gtggtttcgg cttatgcgtt cagcgccggc atttctggct tgttgaccgc
     7261 aggtttcgcc gaccgcttcg atcgcaaaaa gctgctgttg ttcttttata ccggtttcat
     7321 catcggcaca ctgtggtgcg gacttgcaca aagctttgag agcctgctgg cggcgcgcgt
     7381 cgtcaccggc ctgttcggcg gcgtgatcgg ttcgatcgtg ctggctattt cgaccgatct
     7441 gttcccgcta cagatgcgcg gtcgtgtgat aggcatcatc cagacggcat tcgctgccag
     7501 ccaggtgctg ggcattccca tcagcctgta tctgtcgaat cgctggaact ggcatatccc
     7561 gtttatcgcg atggcagcct ttagcatcat aggcggcgtc atcatcatgc tgaaaatgca
     7621 agctgtcgcc gatcatctga acataccgca ggaacactct gcatggaggc atctattcca
     7681 tactgtaacc gagccgcgct atctgaccgc tttcgcgacg accgctctgc tgacgactgg
     7741 cggcttcatg ctgatgccat tcggcagcgc ctatctggtg tcgaatctag gcatcgatat
     7801 tcagcatttg cctaccgttt atctgatcgc cggactatgc accatcggca tcggaccgct
     7861 gatcggcaca gcctccgaca agttcggcaa gttccgcata ttcctgtttg gcacgctgct
     7921 gtcgatcagc atagtgctga tctataccca tctcggccgc actgccatgc cgctggtgat
     7981 cgttgtcaac gtggtgatgc ttctcgggat tttttcgcgc atgattccgt ttcaggcatt
     8041 ggtatcctcc gtgccgatgg tcaatcagcg cggctccttc aatgccgtca gcgcatccat
     8101 tcagcaattg tcggggggcc tcgcctcggt cgcggcaggc cgcatcattt cgctcggcgc
     8161 cgatggtaaa ttacagaatt tcgacatcgt cggctatgtc ttcatcggca cgtcactgat
     8221 ggcattggcg ctggtctggc gactcagccg ccatctcgaa aaccacactt gaaaagccgt
     8281 gaccaagctt caggatgaag ggtaaggcac ccctccaatg aagccaaaat catcgatgca
     8341 gagtggaaaa taccagtatc cggtgacatc aggatattgg taggcacgat tggactcgaa
     8401 ccaacgaccc ctaccatgtc aaggtagtgc tctaaccagc tgagctacgc gcctagtgaa
     8461 gaagcaggat tatagggaaa gtttttattt tttgccacag tcttcatgaa aaaaattcgt
     8521 catcaaacct atcgcccgct actggcgtcc ggcctccagc acatcttcga cttcccggat
     8581 cacggccagc gccttttgca atggcgcggt cgaaccgatt tccgcagtga aactcatgcg
     8641 cgcctgccct ttcgcactcc gaatgcgaac accgatgacg ttgacctttt cgcgcaggaa
     8701 aatatctgaa atatcgcgca gcaaaccctg gcgctccccg gccagtacga aaatgtcgac
     8761 cggatagacc gtatccccgg ccggcgttcc ccatgtggtc tgaatgatgc gctcgggcgc
     8821 cttgctgtgc atttctgcaa gattcttgca attcgagcga tggatagaca cacccttgcc
     8881 acgcgtgacg aaaccagcga tgctatccgg cggcaccggt ttacaacagc gcgccagttg
     8941 cgtcatcaag ccttccgtac caaccaccag cacgcccggc ctgacgccct gcatcacact
     9001 ggaagcgcgg ctcttgcgcg tgatacaggc ttcttccacc tgcttcaccg cctcgcctgc
     9061 acgcaagata tgctcgatct gacgtgaact gaacgcatcc ttgcccagcg ccaggcaaag
     9121 atcgtcaacc ttggcaaagc cgagcctgtg cgcaagttct tcaagattga tagcagttct
     9181 gccctcgcgc tgcaatgttt tttccaggat ggtacggccg tgggccagcg tttcctgttg
     9241 tttgacagca ttgaaccagg cgcgtaattt ggcgcgcgcg cgtgaactga cgacgtagcc
     9301 cggactgagc caatcgcgcg acggaccagc gccgggatca ttcctgacag tgatgatatc
     9361 gatggtctga ccgtttttga gcggcgtatt cagcggcacc atgataccgt cgacgcgcgc
     9421 gccgcggcag caatgcccaa tgtcgctgtg caggcaatag gcaaagtcga taggcgtggc
     9481 gccattcggc aactcgatca cgcgcgcttg cggcgtcagc acgtagatac gatcatccag
     9541 cgtagtcgat ttgagtttcg cgacccagtc acggcgcgct tcctcctgtt ccaccacagc
     9601 accggcgact tcgctcttcc atgccagcaa ctggcgcagc caggcgatct tttcgtcata
     9661 cttctgcgcg gaaaaactgg aaccgccgct ctccttgtag cgccagtgcg ccgccatacc
     9721 gtattctgcg aagtgatgca tctcatgtgt gcgaatctgc acttccagcg gccggccatc
     9781 ttctgcaatc accaccgtat gtagggactg ataagcattt tgcttgggcc gcgcaatgta
     9841 atcatcgaac tcctcgggaa ccggcgtcca gatatcatgg acgataccga gcaccgtata
     9901 gcaatccttg acatcgtcgg tgatgacgcg gaaagcccgc acatcgtaaa gatcggaaaa
     9961 cgccaatgcc ttggcgcgca tcttgttcca gatactataa atgtgtttgg gacgcccgga
    10021 gacttccgcc ttgatctctg ctgctgtcag ttcggtgcgt aaatgctcga tcgcgcgggc
    10081 gacgaattct gtgcgctcca tgcgtttttc ttccagcatc ctggcgatgc gcctgtaagt
    10141 tgccggctcg aggaagcgga acgacaaatc ctccaattcc cacttgagcg gccagatgcc
    10201 aagacgattg gccagcggcg catataaatc gaaagtcgca cgcgcatatt gtgtcgtgat
    10261 gtcattttcc agcctgtttt cggcaaaatg acgcagcgtc gtcacgcgcg cagccaggcg
    10321 caacagcacc acccgc
//
LOCUS       DASRDH010000259         4801 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_112457,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000259 DASRDH010000000
VERSION     DASRDH010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 4801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4801
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112457"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            14..223
                     /locus_tag="VFA14_06065"
     CDS             14..223
                     /locus_tag="VFA14_06065"
                     /inference="COORDINATES: protein motif:HMM:NF025005.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KTSC domain-containing protein"
                     /protein_id="HZS80974.1"
                     /translation="MERTPVSSSNVNSIGYDADSQTLEVEFNNGAVYQYSGVPEYEYD
                     GLMNADSKGKYLHSNVKNRYSFSKL"
     gene            complement(192..1141)
                     /locus_tag="VFA14_06070"
                     /pseudo
     CDS             complement(192..1141)
                     /locus_tag="VFA14_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004350887.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
     gene            complement(1138..2059)
                     /locus_tag="VFA14_06075"
                     /pseudo
     CDS             complement(1138..2059)
                     /locus_tag="VFA14_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517003.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(2306..3751)
                     /locus_tag="VFA14_06080"
     CDS             complement(2306..3751)
                     /locus_tag="VFA14_06080"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008448279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HZS80975.1"
                     /translation="MSKKLTTSAGAPVADNNNIQTAGPRGPALLQDVWFLEKLAHFDR
                     EVIPERRMHAKGSAAYGTFTVTHDITRYTKAKIFSKVGKKTDLFLRFSTVAGERGAAD
                     AERDIRGFAIKFYTEQGNWDLVGNNTPVFFMRDPHKFPDLNRAVKRDPKTNLRSANNN
                     WDYWTLLPEALHQVTIVMSDRGLPRSYRHMHGFGSHTFSFINAANERFWVKFAFKTQQ
                     GIKNLTDAEAQDLVGRDRESAQRDLFDSIEKGDFPRWKLFVQIMPEKEAGSYHINPFD
                     LTKVWPHKDYPEIEVGVLELNRNPENYFAEVEQAAFSPANVVPGIGFSPDKMLQGRLF
                     AYGDAHRYRLGVNSYQIPVNAARCPVHTSHRDGAMRVDGNHGATLGYEPNSYGEWQEQ
                     PDFREPPLAIEGAADHWNHRVDDDYYSQPRALFRLMTPAQQQVLFENTARAINGASEP
                     VVQRHIDNCTKADPAYGAGVAKAIAALKKKG"
     gene            complement(4160..>4801)
                     /locus_tag="VFA14_06085"
     CDS             complement(4160..>4801)
                     /locus_tag="VFA14_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006464127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HZS80976.1"
                     /translation="LRARWESFRQMRATTHATQQQEPVLAGLVVRDNLRNRRTIQRAT
                     MQALGQARDAAFLVSPYFAPGRKLCHALEEAAGRGVRVTLLLGIGQFYLQDAVAHSYY
                     SRLLKAGVRIVEYTRTELHAKVAVIDDTWATVGSSNYDGLSLFVNQEANVLINDVDFA
                     SKLRAEIEAGISNGRLVRLQDVLHAPRYRRFWYGVVSLLYRGIIHVITLGRDA"
BASE COUNT          974 a         1409 c         1389 g         1029 t
ORIGIN      
        1 aaggagactc tccatggaac gcacccctgt tagttcaagc aatgtcaact caattggcta
       61 tgacgcggat agccagactc tcgaagttga attcaacaat ggtgccgtat atcaatactc
      121 gggtgttccc gagtacgagt atgacgggct catgaatgct gactccaaag ggaagtattt
      181 gcattcgaat gtcaagaatc ggtactcctt ttcgaagctg tagattgcgc cccgccagcg
      241 gcgcgttgct cgatctgcaa ctgcgcccgc tgcgttgcct gatgtagccg ttccaccaac
      301 atcgcacgcg gcggcaaggt ggtcaggtac ttggcaacgt ggatgcccga tttgtccaat
      361 tccagcagtt cgatctgctc ggacttcttg ccggtgcaga ggatgatgcc caacggcgcg
      421 gcttcctccc gctcgaactt gtcgagccag cgcaaataga gctccatctg ccccttgtag
      481 gctgccttca attcgaccgc caccagtcgg cgcagcttgc ggttgtaaaa cagcaggtcg
      541 aggtgaaaat cctcgccgtc gatctggatg cgcttctgcc gggccacgaa agtgaagccc
      601 acgcccagct ctagcagaaa ggattccatt tcgcggatga tcgccgcctc caagtcgcct
      661 tcctgctagg tgtctcgcag gccgaggaaa tcgaggatgt acgggtcgcg catgaccagg
      721 gcgggcgaca tgcgctgcgc atcgcgcatc gtcgccagct cctgcgcgat cgtctcgccc
      781 ggcttcttgg acagcgcagt gcgctcgtag agcatcgagt cgatgcgctc gcgcagcgtc
      841 cgcacgctcc agcgttcggc gctcgtcatc tgcgcgtagt agtcccgctg gaacgggtct
      901 ttcagcggca tcagcgcgat gaagtgcgtc cagctcaatt ctcgtatcag tgatacgaga
      961 atctgctcgt cggggaaggt ggccgcgaac tgcaccatgc ggcgcaggtt ctgctcggca
     1021 aaactgctgc cgtactcctg caccaactgc gccgctaggg tcggcaacac ttccttgcca
     1081 taggcgccgc ggcacccgtc caagacttgc gctagccgat gcgccaatag agcatcgtca
     1141 tcgaggcggc gcgcaagcgc gccgattcgc caccagttca gccgctttgc gcaccgggtt
     1201 atcctcggtg tggacatacc gcatgaacat cgccacggtc ttgtgcgcgg tcagcgccat
     1261 gccgaccttg accgggatgc ccgagttggc aatgtccatg gccgaacggt ggcggatgcc
     1321 gtgcgtgccc acatgcgccg cgccggccgc cttaagcgcg cggctccagc cgccgtaata
     1381 ctcgcccgtg gtcaggtgct tgccgggatg gttcggcgac ggcagcacat ggggattgcc
     1441 ttcctgtctc ggcgccgtcg aaagcagccg gtaggcttcc tcgctcatag gcttggacat
     1501 accgccggtc ttgctgtcgg gccagaccac gcggcgattc tccaagtcca cccaatccca
     1561 ttggagcgtg acgatttcgg agcggcggcc tgcaaactcg aactgcaggc ggatggctag
     1621 gggaatgacg tagttctcta gcccctcggc ctcgatccgg ccaagatgcc ggaacagctt
     1681 gcccatgtcc tcgtcgctga tgaggtgcgt ggccttgccg ttgcctgggg ttgtcctcca
     1741 taaacttggc acacagatcc ttgaccgtgg gggctgcgcg ggcggcggcc ttggcggcgc
     1801 tgggatcgcc gccccggcgc acctcggcca gccattcctg ggccagagag cgcgcttgtt
     1861 cgacggtcag ttccccgtac aggcccaagg cgggcttgcg gcgctcaccg gcggtgcggt
     1921 actggagcat gaacaccttg cggcccgccg gtgtaacctt gcataggaag ccgggcacca
     1981 gcgtgtcccg gagttcgacg gcctgcgcca ggggttgcgc tgcatcgact gcggacttgg
     2041 tgagcttgag ttttgccacg atgactcctc ggaaagaccc agttcccagg agccttgtag
     2101 gggccagcag agcggaagcc gggtcaggtt ttggaaagca ccggcctatg ttgaactcgc
     2161 ctaagttatt gataaacccg ctgtatcagg cttcggcgta gtccagcgaa gttcggtgct
     2221 ggagtcatga tgaaatgaaa aatgccgctg tcttgtgaga cagcggcatt ccggggcctg
     2281 aaaactgtca gcagtctttc taccgtcagc ccttcttctt gagcgcggca atcgctttcg
     2341 cgacaccagc gccgtaggct ggatcagcct tggtgcaatt gtcgatgtga cgttgcacga
     2401 ccggttccga cgcaccatta atggcccgtg cggtattctc gaacagcacc tgctgctgcg
     2461 ctggcgtcat caaccggaac aaggcgcgcg gctgcgagta atagtcgtca tcgacacgat
     2521 ggttccagtg atcggcagcg ccttcgatgg ccagcggcgg ctcacggaag tcgggttgct
     2581 cctgccattc gccgtaacta ttcggctcgt aacccagcgt cgcaccgtgg ttgccatcga
     2641 cgcgcatggc accatcgcga tgcgaggtat ggaccggaca acgtgcggca ttgaccggaa
     2701 tctggtagct gtttacaccc aggcggtaac gatgcgcatc gccgtaggca aacagacgac
     2761 cttgcagcat cttgtccggc gagaagccga tgcccggcac cacattggct ggcgaaaagg
     2821 cagcctgttc gacttccgcg aagtaattct ccgggttacg gttgagttcc agcacgccga
     2881 cttcgatttc cggataatcc ttgtgcggcc acaccttggt cagatcgaac gggttgatgt
     2941 gatagctgcc agcttccttt tcaggcatga tctgcacgaa tagcttccag cgcgggaaat
     3001 cgcccttttc gatgctgtcg aacaaatcgc gttgcgcact ttcgcggtcg cgcccgacca
     3061 gatcctgggc ttcggcatcg gtcaggttct tgatgccttg ttgggtcttg aaggcgaact
     3121 tgacccaaaa gcgttcattg gcagcattga tgaagctgaa ggtgtggcta ccaaagccat
     3181 gcatatgacg atacgaacgc ggcaaaccgc ggtcgctcat gacgatggtg acctgatgca
     3241 gcgcttccgg cagcagtgtc cagtaatccc agttattgtt ggcgctgcgc aggttggttt
     3301 tcggatcgcg cttgacagcg cggttcaggt ctggaaattt atgcggatcg cgcatgaaaa
     3361 atactggcgt gttgttgccc accagatccc agttgccttg ctcggtataa aacttgatcg
     3421 cgaaaccgcg gatgtcgcgc tcagcatcag ccgcgccgcg ttcgccggcc acggtagaaa
     3481 agcgcaggaa caagtcggtt ttctttccga ctttcgagaa aatcttggcc ttcgtgtaac
     3541 gcgtgatgtc atgcgtcacg gtgaaggtgc cataagctgc cgaacctttg gcatgcatgc
     3601 ggcgctcagg aatcacttca cggtcgaaat gagccagttt ttcgagaaac cagacatcct
     3661 gcagcaaagc gggaccgcgt gggccggcag tctggatatt gttgttatca gcaaccggtg
     3721 cgcctgcgga agtggttaat ttcttgctca tgtcatatcc cttgaaaaat cgtcaagtaa
     3781 aaaagcctcc ataccgacga actgctgatc gctgatatgg tctcgaatga attttaaata
     3841 aattgattga atattataaa ttgattgttt ttattaattg aataagtatt ctgtgtttcg
     3901 gcggatgtca tgcatccagc catcaaaaac gcaacaatat acaacccttc ctttaaataa
     3961 aaagacaatg acaggcaaag aaaattccac acggatcgcc gggaatctgc atgcaacatg
     4021 agccgcattg ctcgcagtac atcaccttgc cggcgggctt cagatgcagc gcggtgccca
     4081 tagccaaggc catcaggcca cttgccccgg ttgaggtggc gacccgattc tcctcgcacg
     4141 tcatcggaat tttttcgttt caggcatccc gcccgagcgt gatgacatgg atgatgccgc
     4201 gatacaacag ggaaacaaca ccataccaga accgcctgta gcgcggcgca tggagaacat
     4261 cctgtaaccg gaccaggcgc ccattgctga tccctgcttc gatttcggcg cgcaacttgc
     4321 tggcaaaatc gacatcgttg atcagcacat tggcttcctg attgacgaac aggctgagac
     4381 cgtcataatt gctggaaccg accgtggccc aggtatcgtc gattaccgcc accttggcat
     4441 gtaactcggt cctggtgtat tcgacgatgc gcacaccagc cttgagcagc ctggaataat
     4501 aggagtgtgc caccgcatcc tgcaggtaaa actgtcctat gccaagcagc agcgtcacgc
     4561 gcacgccacg cccggcggcc tcttccagag catggcataa cttgcgtccg ggcgcgaaat
     4621 acggactgac aagaaaagcc gcatcgcgcg cctggcccag cgcctgcata gtcgcgcgtt
     4681 ggatggtacg ccggttacgc aggttatcgc gaactaccag ccctgccagg accggttcct
     4741 gctgctgtgt tgcgtgggta gtcgcgcgca tctggcgaaa gctttcccag cgcgcgcgca
     4801 g
//
LOCUS       DASRDH010000260         7449 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_504772,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000260 DASRDH010000000
VERSION     DASRDH010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 7449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7449
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_504772"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..832
                     /gene="glyQ"
                     /locus_tag="VFA14_06090"
     CDS             <1..832
                     /gene="glyQ"
                     /locus_tag="VFA14_06090"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HZS80977.1"
                     /translation="GAGTSHTATFLRAIGPEPWRAAYVQPSRRPRDGRYGENPNRMQH
                     YYQYQVVLKPAPETILDLYLASLEALGLDLKQNDVRFVEDDWENPTLGAWGLGWEVWL
                     NGMEVTQFTYFQQVGGLDCKPVLGEITYGIERLAMYLQGVENVYDLVWTEWVENDMKR
                     KLTYGDVFHQNEVEQSAFNFEYSNTAFLFSLFGNYESEARRLLEVPLALPAYEMILKA
                     AHTFNLLDARGAISVTERAAYIGRIRNLSRAVAQAYFGSRERLGFPMLDATDTSRQAA
                     "
     gene            851..2962
                     /gene="glyS"
                     /locus_tag="VFA14_06095"
     CDS             851..2962
                     /gene="glyS"
                     /locus_tag="VFA14_06095"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008325693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HZS80978.1"
                     /translation="MTQTLLVELLTEELPPKALAGLGDAFADSIFSGLKTRGFLADDA
                     VATAYATPRRLAVSISQVHAASPDQTIREKVLPVSIALDAEACPTAPLVKKLAALAAR
                     VGATVITPDQLERAQDGKSESLFYHYTARGAVLQDGLQVVLEETVAGLPIPKLMSYQR
                     QQGHDAGSTVRFVRPAHRLVALYGDTVLPLRILGLDAGRITQGHRFLSQGAITIDSAA
                     AYVETLTGAGKVIPGFSARKEKIRLDLLAQAKDDQVLMPEPLLDEVAALVEWPVVYTC
                     RFDDRFLAVPQECLILTMQTNQKYFALTDAAGRLRPRFLIVSNLETSIPQHIVQGNER
                     VVRPRLADAKFFFEQDKKKSLAERVPQLADVVYHNKLGNQLQRTERVKALAGAIARLL
                     AQDAALAERGALLAKADLLTDMVGEFPELQGIMGNYYARHDGEPDEVALAVSEHYQPR
                     FAGDALPGSATGTVVALADKLETLVGIWGIGLQPTGDKDPFALRRHALGILRMLIEKR
                     LPLPIPLLLQEAASLFAGNDNFRDPRADLSTFMYERLRSLLRERNYSPYEIEAVVAQQ
                     PAVLNDIAERLDAVRAFTALPEAEALASANKRISNILKKIADSKPGAVQSGLLHEPAE
                     EALYSAMLLLKPQVDAAYTRGDFSGALKALAQLRQHVDTFFNDVMVNVEDEQLRRNRQ
                     ALLTGLHQMLNQVADISRLAA"
     gene            2979..3533
                     /gene="gmhB"
                     /locus_tag="VFA14_06100"
     CDS             2979..3533
                     /gene="gmhB"
                     /locus_tag="VFA14_06100"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HZS80979.1"
                     /translation="MKLIILDRDGVINHDSDTFIKSPEEWLPIAGSLEAIARLNQAGY
                     RIVIATNQSGVGRGLFNMMTLDAIHQKMHHAAQQVGACIDAVFFCPHTANDNCDCRKP
                     REGMFHDIARRFGIDLRSTATVGDSLRDLQAGFKAGCVPCLVLTGKGEKTREKGGLPP
                     GTLIYPNLAGVVDFILKTPAEIPV"
     gene            3625..4332
                     /locus_tag="VFA14_06105"
     CDS             3625..4332
                     /locus_tag="VFA14_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HZS80980.1"
                     /translation="MATATIVWSMVCILFAPFPYARRYYLTSRWNVIVIWAARVICGI
                     RHQIKGMENLPDAPVILLSKHQSTWETIFYMIMMPRPLVFVFKKELTYIPFFGWGIAL
                     LRMIPIDRGKGRDAFAQVLEQGRQRLATGQWVIMFPEGTRIPVGQAGKYKSGGARLAV
                     ETGTPVVPIAVNAGECWPKKSFIKTPGIITVSIGKPISPEGLNAMQLIQHVENWIESE
                     MHMISPAAYAAIDKTNG"
     gene            4352..5212
                     /locus_tag="VFA14_06110"
     CDS             4352..5212
                     /locus_tag="VFA14_06110"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HZS80981.1"
                     /translation="MKLLRHSQPDSGQLTLQLDFFTPAPPETEQDAASSRPMPPPIAR
                     PATGMRRIRLSEHHLDYTLLRSRRRSIGFLINDKGLRVTAPKWVTLAEIENAIRENQR
                     WIFARLDEQSSARRLQTQRQWHDGATLPYLGQHLALRIHAGQADASVAYDETALVLTI
                     CLPADAGEQQLKHLVQDWLQTRAQETFAARLPVYAGKLGVACRSLALSSASTRWGSCT
                     ADGRIRLNWRLIHCALPVIDYVIVHELSHLQEMNHSPRFWATVRSAFPECEAAKRALR
                     ESAPEILSIF"
     gene            complement(5354..5680)
                     /locus_tag="VFA14_06115"
     CDS             complement(5354..5680)
                     /locus_tag="VFA14_06115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS80982.1"
                     /translation="MLSAALFGLATGAAEPLADRVGRKIVLTAGFVFCIGQGISNLTV
                     LGAAIFLAGIVMNGAQTAMPALAAQFYPTQGRATGVAWMLGIDRCGGIAGALLGAELM
                     PPAYWL"
     gene            complement(5674..5823)
                     /locus_tag="VFA14_06120"
     CDS             complement(5674..5823)
                     /locus_tag="VFA14_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS80983.1"
                     /translation="MTNSASFDIQQFIERQPLGAFHWVVLSLCFSVLVVDGFDTAAIG
                     YIALC"
     gene            complement(5837..6622)
                     /gene="rsmA"
                     /locus_tag="VFA14_06125"
     CDS             complement(5837..6622)
                     /gene="rsmA"
                     /locus_tag="VFA14_06125"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HZS80984.1"
                     /translation="MKHIARKRFGQNFLTDQTVLDDIISVIAPQKEDAMVEIGPGLAA
                     MTALLLESLDHLHTVELDRDLVARLRKRFDPKRLQVHAADALQFDFTSIPVASGRKLR
                     VVGNLPYNISSPLLFHLAPLASCVQDQHFMLQKEVVERMVAEPGTKAFGRLSVMLQWR
                     YHMSLLFVVPPTAFDPPPRVDSAIVRMIPLAQPLECQQSLLEEVVTRAFSQRRKLIRN
                     GLAGLLTEADLIDAGIDPQARPEAVPTAQFVALANALGKKRRA"
     gene            complement(6619..>7449)
                     /gene="pdxA"
                     /locus_tag="VFA14_06130"
     CDS             complement(6619..>7449)
                     /gene="pdxA"
                     /locus_tag="VFA14_06130"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HZS80985.1"
                     /translation="RNTGLPDLPQDRLIVIDCPADVPPTPGVPDARNGHAVLQTLDLA
                     VEGALQQRFEAIVTAPLQKSSINDAGVPFTGHTEYLAQRTATPHVVMMLAGGAAPHLR
                     VALASTHLALKDVPAAITRAGLGRTLDIIHAGLRDQFGIARPRILVAGLNPHAGENGY
                     LGREEIDVIIPALTAAKERGMDVIGPYPADTLFQKKYLDHADCVLAMYHDQGLPVLKF
                     ASFGQGVNITLGLPIIRTSVDHGTALDLAARGTGMADHGSMIAAVETAALMVRARHAR
                     "
BASE COUNT         1569 a         2305 c         2166 g         1409 t
ORIGIN      
        1 cggcgccggc acctcgcata ccgcaacctt cctgcgcgcc atcggtccgg aaccctggcg
       61 tgcggcgtat gtgcagcctt cgcgccgtcc cagggatggc cgctacggcg aaaacccgaa
      121 ccgcatgcaa cactactacc agtatcaggt ggtattgaag ccggcgccgg aaactatcct
      181 cgacctgtac ctcgcttcgt tggaagcgct tggcctggat ctgaagcaga acgatgtgcg
      241 cttcgtcgaa gacgactggg aaaaccccac cctcggcgcc tggggcctgg gctgggaagt
      301 ctggctcaat ggtatggaag tgacgcagtt cacctacttc cagcaggtcg gtggcctcga
      361 ctgcaagccg gtactcgggg aaatcaccta tggcatcgag cgtctggcga tgtatctgca
      421 aggcgtggaa aacgtttacg atctggtctg gaccgaatgg gtcgagaatg acatgaagag
      481 aaaactgact tacggcgatg tattccatca aaacgaagtc gagcaatcag ccttcaactt
      541 cgaatactcg aataccgctt tcctgttctc gctgttcggc aattacgaat ccgaagcccg
      601 gcgtctgctg gaagtcccgc tggcactgcc ggcttacgaa atgattctga aagcagcgca
      661 caccttcaac ctgctggatg cacgcggcgc catttccgtt accgaacgcg ccgcttacat
      721 cggtcgcatc cgcaatctgt cacgcgccgt ggcgcaggca tatttcgggt cacgcgagcg
      781 cctcggcttc ccgatgctgg acgcgacgga cacctcccgc caggcggcct gacaagcagg
      841 aaaacagaaa atgacgcaaa cactcctcgt agaactcttg accgaagaat tgccgcccaa
      901 ggcgctcgcc ggactaggcg acgcatttgc cgacagcatt ttcagcggcc tgaaaacacg
      961 cggtttcctg gccgacgatg cagtcgccac ggcttacgca acgccgcgcc gcctggcggt
     1021 ttccatcagc caggtacacg ccgcgtcacc ggatcagaca atccgtgaaa aagtgctgcc
     1081 ggtgagcatc gcactcgacg ccgaagcctg tccgacggcc ccgctggtca agaagctggc
     1141 cgcgctggct gcccgggtcg gcgccactgt gatcacaccc gatcaactgg agcgcgcaca
     1201 ggacggtaag agcgaaagct tgttctacca ctacaccgcc agaggcgcgg tgctacagga
     1261 cggcttgcaa gtcgtcctgg aagagacggt tgccggactg ccgattccca aactcatgag
     1321 ctatcagcgt cagcaaggcc atgatgccgg cagtaccgtc cgcttcgttc gcccggcgca
     1381 tcgcctggtc gcattgtacg gtgatacggt gctgccgctg cgcattctcg gtctggatgc
     1441 cggcaggatc acgcaaggcc atcgcttcct gtcacaaggc gcgatcacga tcgactcggc
     1501 agcggcgtat gtcgagacgc tgaccggcgc cggcaaggtc attccgggct tcagcgcgcg
     1561 caaggaaaag attcgcctcg acctgctcgc ccaggccaaa gacgatcagg tgctgatgcc
     1621 ggagccgctg ctcgacgaag tggcagcgct ggtcgaatgg ccggtcgtct acacatgtag
     1681 gttcgacgat cgattcctgg ccgtgccaca ggaatgcctg atcctgacca tgcagaccaa
     1741 tcagaaatat tttgcgctga ccgatgccgc cggccgtctg cgcccacgct ttttgatcgt
     1801 ctccaacctg gaaacctcca tcccccagca catcgtgcaa ggtaatgagc gcgtcgtgcg
     1861 tccgcgcctg gcagatgcga aattcttctt cgagcaggac aaaaaaaaat cgctggccga
     1921 gcgcgtgccg caactggccg acgtggtgta tcacaacaag ctgggcaatc aactgcaacg
     1981 gacagagcgc gtcaaggcgc tggccggcgc gatcgccagg ctgctggctc aggatgcagc
     2041 gctggctgaa cgcggcgcct tgctggccaa ggccgacctg ctgaccgata tggtcggcga
     2101 gtttcccgag ctgcagggca tcatgggtaa ctactacgcg cgtcacgatg gcgaaccgga
     2161 tgaggtcgca ctggccgtct ccgaacacta tcagccgcgc ttcgctggcg acgccctgcc
     2221 tggctcggca accggcaccg tcgtcgcgct ggccgacaaa ctggaaaccc tggtcggcat
     2281 ctggggcatc ggtctgcaac cgaccggcga caaggatccg ttcgcgctgc gccgtcacgc
     2341 gctgggcatc ctgcgcatgt tgatcgaaaa acgcctgccg ctgccgattc cgctcctgct
     2401 gcaagaagct gcatcactgt tcgccggcaa cgataacttc agggatccgc gcgccgacct
     2461 cagcactttc atgtacgagc gtttgcgcag cctgctgcgt gagcgcaatt attctcctta
     2521 tgaaatcgaa gccgtggtcg cgcaacaacc ggccgtactc aacgacatcg ccgagcgcct
     2581 cgatgccgtg cgcgcattca cggcattgcc ggaagccgaa gcgctggcaa gcgccaataa
     2641 acgcatcagc aacatcctga agaaaatcgc cgacagcaaa cccggtgccg tgcagagcgg
     2701 tctgctgcac gaacctgccg aagaggcgct gtattcggcc atgctgctgc tgaagccgca
     2761 agtcgatgcc gcgtatacgc gcggcgattt cagcggcgcc ctgaaagcgc tggcgcaact
     2821 gcgccagcat gtcgatactt ttttcaacga cgtcatggtc aatgtcgaag atgagcaact
     2881 gcgccgcaac cgtcaggctt tacttaccgg cttgcatcag atgctcaatc aggtggcgga
     2941 catttccagg ctggcggcat gattcccgga caaaaaacat gaagctgatt attctggacc
     3001 gcgatggcgt gatcaatcac gactcggaca ccttcatcaa atcgcccgag gaatggctgc
     3061 cgatcgcagg gtcgctggaa gccatcgccc gcctcaacca ggccggctat cgcatcgtca
     3121 tcgccaccaa tcaatcgggc gtcggccgcg gcctgttcaa catgatgaca ctcgatgcga
     3181 ttcaccagaa aatgcaccat gccgcgcagc aggtcggcgc ctgcatcgat gccgtattct
     3241 tttgcccgca caccgccaac gacaattgcg attgccgcaa acccagggag ggcatgttcc
     3301 atgacatcgc ccgccgcttc ggtattgatc tgcgcagtac cgccaccgtg ggcgattcac
     3361 tgcgcgatct gcaggccggc ttcaaggccg gctgcgtacc ctgtctggtg ttgaccggca
     3421 aaggcgaaaa aacccgggaa aaaggcggac tgccgcccgg cacgctgatt tatcccaatc
     3481 tcgccggggt ggtcgatttc atattgaaga caccagcgga aatcccggtc tgaagctgcc
     3541 gccgcagcct gtgccggact tcacgaggaa gactgaatct tgcgtatcga tctgcttttg
     3601 cgttctcttc tgttcacgct agtcatggca acagcgacca tcgtctggtc gatggtctgc
     3661 atcctgtttg caccgtttcc ttacgccagg cgctattacc tgacctcgcg ctggaacgtc
     3721 atcgtgatct gggcggcgcg tgtgatctgc ggcatccgtc atcaaatcaa ggggatggaa
     3781 aacctgcccg acgcaccggt gatcctgctg tcgaaacatc aatcgacgtg ggaaacgatt
     3841 ttctacatga tcatgatgcc gcgcccactg gtgttcgtgt tcaagaaaga attgacctat
     3901 attcctttct tcggctgggg catcgccttg ctgcgcatga tccccatcga ccgcggcaag
     3961 ggccgcgacg ccttcgccca ggtactcgag caaggtcgcc agcgcctcgc cacaggccag
     4021 tgggtgatca tgttcccgga gggtacacgc attccggtcg gccaggccgg caagtacaag
     4081 tcgggcggcg cgcgcctggc ggtggaaacc ggcacgccgg tcgtaccgat cgccgtaaac
     4141 gctggcgaat gctggccgaa aaaatcattc atcaaaacgc cgggtatcat caccgtctcc
     4201 atcggcaagc caatttcgcc ggaggggctg aacgcaatgc aattgatcca gcacgtggaa
     4261 aactggatag aatccgaaat gcacatgatt tccccggcag cctatgccgc catcgataaa
     4321 acaaacgggt agcgctcgtt tcgccaatac attgaaactg cttcgtcact cacaaccaga
     4381 ctccggtcaa ctcacactgc aactggattt cttcacccca gcgccgcctg aaacggagca
     4441 ggacgccgca tcatctagac cgatgccgcc acccatcgct cgcccggcaa ccggcatgcg
     4501 ccgcatccgg ctgagcgaac accacctcga ttacaccttg ctgcgttcca ggcgccgctc
     4561 tatcggtttt ttgatcaacg acaaaggcct gcgtgtgacc gcgccgaaat gggtgacgct
     4621 agcggaaata gaaaatgcca ttcgcgagaa tcagcgctgg atcttcgcca ggctcgacga
     4681 gcaaagttca gcgcgccgcc tgcaaacgca gaggcaatgg cacgacggcg cgacgctgcc
     4741 ttatctcggc caacacctcg cactgcgcat ccatgccggc caggctgacg caagcgtcgc
     4801 ctacgacgaa acggcattgg tattgacgat atgcctgccc gccgacgctg gcgaacagca
     4861 attgaagcat ctcgtgcagg actggctaca gacgcgcgct caggaaacct ttgccgcgcg
     4921 gctgccggtc tatgccggga agctgggtgt cgcctgccgc tctttggctc tgtcgtcggc
     4981 aagcacacga tggggttcat gcacagccga cggccgtatt cgcctgaact ggcgcctgat
     5041 acattgcgcc ttgccggtga tcgactacgt gatcgtgcat gaactgtcgc atctacaaga
     5101 aatgaaccac agtccacgct tctgggccac ggttcgatcg gctttccctg aatgcgaagc
     5161 ggcaaaacgg gcattgcgcg agagcgctcc ggaaatactg tccatttttt gaacaggaac
     5221 gcgcgaaatc gcgctctacg agatcatcat tgttcgcgag cgactgcttt ttttaccccc
     5281 acttgctgcg agcttgacga caagcgcaat tgtcgcgact gctgccggga tactggcgag
     5341 tatgaagatc atgtcaaagc caatatgcag gcggcatcaa ctccgctccc agcaatgctc
     5401 cggcgatgcc gccacacctg tcaataccca gcatccaggc aacgcctgtc gccctccctt
     5461 gagtcgggta gaactgggct gcgagagcag gcatcgctgt ttgcgcgccg ttcatgacga
     5521 ttccggccag gaagatcgct gcgccaagca cggtcagatt ggagattccc tgaccgatgc
     5581 aaaagacaaa accggcagtc aagacgattt ttctaccgac ccggtccgcc agaggttctg
     5641 ctgcgcctgt cgccagtccg aacagcgccg cgctcagcat agtgcaatat agccaatcgc
     5701 ggcggtgtcg aatccatcga cgaccagcac tgaaaagcag aggctcaaca cgacccagtg
     5761 aaatgcgcca agaggctgcc tctcgatgaa ctgctggatg tcgaatgaag cgctgtttgt
     5821 catgttgtct ccatcttcag gcgcggcgtt ttttcccgag cgcattcgcc agggcgacga
     5881 attgcgcggt cggcactgct tccggccgcg cttgcgggtc gatgccggca tcgatcagat
     5941 cggcttcggt cagcagcccc gcaaggccat tgcggatcag cttgcggcgt tgcgagaacg
     6001 ccctggtcac gacttcttcc agcagggact gctggcattc cagcggctgc gccagcggaa
     6061 tcatgcggac gatggcagag tccacgcgtg gcggcggatc aaaggcggtc ggcggcacca
     6121 cgaacagcaa gctcatgtga tagcgccatt gcagcatcac cgagaggcga ccgaatgcct
     6181 tggttccagg ctcggccacc atgcgctcga cgacttcctt ttgcagcatg aaatgctgat
     6241 cctgcacaca ggacgccagt ggcgccagat ggaacagcaa agggctggaa atattatagg
     6301 gcagattacc gaccacgcgc agcttgcggc cggacgctac cgggatcgag gtgaaatcga
     6361 attgcagcgc atcggcggca tgcacttgca agcgcttcgg atcgaatcgt ttgcgcaagc
     6421 gcgcaaccag atcgcgatca agttcgaccg tgtgcagatg atccagcgac tccagtaaca
     6481 gggcggtcat ggctgccagg ccgggaccga tctcgaccat ggcgtcttct ttttgcggcg
     6541 cgatgacgct gatgatgtcg tcaagaacag tctgatcggt caggaaattc tggccgaaac
     6601 gtttgcgggc aatatgtttc atcgagcatg tcgggcgcga accatgagcg ccgcagtctc
     6661 gacggctgcg atcatgctgc cgtggtcagc catgccggtg ccccgggcag ccagatccag
     6721 cgccgtgccg tgatcgaccg aagtacggat gatcggcagc ccgagcgtaa tattgacgcc
     6781 ctgcccgaag ctggcgaatt tgagcaccgg caaaccctgg tcatggtaca tggcaagcac
     6841 gcaatccgca tgatccagat attttttttg aaacagggta tcggccggat aaggaccgat
     6901 gacatccatg ccacgttctt tggcggctgt cagcgcggga atgatgacat cgatctcttc
     6961 gcggccgaga tagccgtttt cacccgcatg cggattcagg ccggcgacca gaatgcgcgg
     7021 ccgggcgatg ccgaactggt cacgcaggcc ggcatggatg atgtcaagcg tgcgccccag
     7081 gccggcgcgg gtaatcgctg ccggaacatc tttgagcgcc agatgggtac ttgccagtgc
     7141 gacgcgcaga tgcggcgccg cgccaccggc cagcatcatc accacgtgcg gcgtggcggt
     7201 gcgctgcgcc agatattcgg tatggccggt aaacggcacg ccggcatcgt tgatgctgct
     7261 tttttgcaac ggcgccgtga cgatggcttc gaagcgttgc tgcaaagcgc cttcgacggc
     7321 gaggtccagg gtctgcaaca cagcgtggcc attgcgcgca tcgggcacgc caggcgtagg
     7381 cggcacatcg gcgggacagt cgatgacgat cagacggtct tgcggcaaat cgggcagacc
     7441 ggtgtttct
//
LOCUS       DASRDH010000261         3766 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_847154,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000261 DASRDH010000000
VERSION     DASRDH010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 3766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3766
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_847154"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="VFA14_06135"
     CDS             complement(<1..227)
                     /locus_tag="VFA14_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS80986.1"
                     /translation="MQFSENWLRTMVDPQMSSDALSHLLTMSGLEVEEVEAVAPSFAH
                     VVVGEVLEVSRHPNADRLNVCRVDVKTGTIL"
     gene            complement(273..1286)
                     /gene="pheS"
                     /locus_tag="VFA14_06140"
     CDS             complement(273..1286)
                     /gene="pheS"
                     /locus_tag="VFA14_06140"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008113575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HZS80987.1"
                     /translation="MNSLEQLVAQARADFAGVPDAAALENAKARYFGKTGQITEQMKR
                     LGKLAPEDRKAQGAVINAVKEQIESALVARRDALANAQMQERLDAEAIDVTLPGRGRG
                     KGGIHPVMRSWQRVEEIFRSIGFDVADGPEIETDWTNFSALNSPENHPARSMQDTFYI
                     EGGDSKPLLLRTHTSPMQVRHARMNRPPIKVIAPGRAYRVDSSDATHSPMFHQVEGLW
                     IAEDISFADLKGVYLDFVKTFFEIDDLQLRFRPSYFPFTEPSAEIDIAFGSGPLQGRW
                     LEISGAGQVHPAVVRNMNLDPEQYIGFAFGSGLERLTMLRYGIDDLRLFYEGDLRFLK
                     QFN"
     gene            complement(1439..1798)
                     /gene="rplT"
                     /locus_tag="VFA14_06145"
     CDS             complement(1439..1798)
                     /gene="rplT"
                     /locus_tag="VFA14_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007882754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HZS80988.1"
                     /translation="MPRVKRGVTARARHKKVLAQAKGYRGRRSTVYRIAKQAVMRAGQ
                     YAYRDRRNKKRVFRALWITRINAAVREHGVTYSVFMNGLKRASIELDRKVLADMAVTD
                     KPAFAAIVNQVKAGIAR"
     gene            complement(1819..2016)
                     /gene="rpmI"
                     /locus_tag="VFA14_06150"
     CDS             complement(1819..2016)
                     /gene="rpmI"
                     /locus_tag="VFA14_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HZS80989.1"
                     /translation="MPKMKTKSSAKKRFRVRPGGTVKRGQAFKRHILTKKTTKNKRQL
                     RGAVGVHEADMTSVRAMLPFA"
     gene            2015..2284
                     /locus_tag="VFA14_06155"
     CDS             2015..2284
                     /locus_tag="VFA14_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS80990.1"
                     /translation="MTLSTLFGTAPFLMMTGGSNTLLLDACSHLSATVSTAAFAGRKT
                     IASRQNLRYRYSYRLPYRNTHRNKLPAMRKNMAYFFISSFSAPEP"
     gene            complement(2253..2774)
                     /gene="infC"
                     /locus_tag="VFA14_06160"
     CDS             complement(2253..2774)
                     /gene="infC"
                     /locus_tag="VFA14_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012435921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HZS80991.1"
                     /translation="MATDKSHRINGEINAPELRLSGVDNEPLGIVNLAEAFRLAEEAN
                     VDLVEIAPTAQPPVARLMDYGKFKYQEQKKAHEAKLKQKIIQVKEVKFRPGTDDGDYN
                     IKLRNLTRFLEDGDKTKITLRFRGREMAHQEIGMRVLERLRTDLAPHGQVEQFPKMEG
                     RQMIMVLAPKKKK"
     gene            complement(2840..>3766)
                     /gene="thrS"
                     /locus_tag="VFA14_06165"
     CDS             complement(2840..>3766)
                     /gene="thrS"
                     /locus_tag="VFA14_06165"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013233967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HZS80992.1"
                     /translation="LKPMNCPGHIQIFNSDLHSYRDLPLRYGEFGQCHRNEPSGALHG
                     MMRVRGFTQDDGHIFCTEDQVQGEVAAFDRIVREVYATFGFTDVTVKLALRPEKRIGE
                     EAVWNKAENALREAIRASGTGWEELPGEGAFYGPKIEYHLKDSIGRSWQCGTMQVDFS
                     MPARLGAEYVTEDNERKTPVMLHRAIVGSLERFIAILIENYAGAMPLWLAPEQAVILN
                     ISDAQSEYVMDLAQTLKKQGFRVVTDLRNEKITYKIRQHSLQKPPYILVVGDKERDTN
                     TVAVRARGNVDLGVMPVDVLVERLKSEVEAKA"
BASE COUNT          829 a         1094 c          955 g          888 t
ORIGIN      
        1 ttgagaatgg taccggtctt gacgtcaacc cggcaaacgt tcaagcgatc ggcattggga
       61 tgcctgctca cttccagcac ttcacccacg acgacatggg caaacgacgg cgcgaccgct
      121 tcgacttctt ccacttccag acccgacatc gtcagcaaat gcgacaaggc atccgaactc
      181 atctgcggat cgaccatggt gcgcaaccag ttttcagaaa attgcatgat gaatactttc
      241 agctatcatc cgcctcaaac aacaggatat gttcagttga actgcttcag gaaacgcaaa
      301 tcgccttcat agaacaggcg caggtcgtcg atgccataac gcagcatggt cagccgctcg
      361 aggccggagc cgaacgcaaa tccaatatat tgctcgggat cgaggttcat gttgcgcacc
      421 acggccgggt gtacctggcc ggcgccagag atttccagcc agcggccctg caacggaccg
      481 ctgccgaagg cgatgtcgat ttccgccgac ggttcagtga atggaaaata cgacggacgg
      541 aaacgtagtt gcaagtcatc aatttcaaag aaagtcttga cgaaatcgag gtagacaccc
      601 ttgagatcgg caaaactgat gtcttcggcg atccacagac cttcgacttg atggaacatc
      661 ggcgagtgcg tggcatcgct gctgtcgacg cggtaggcgc ggcccggtgc aatgaccttg
      721 atcggcggcc tgttcatgcg cgcatggcgc acctgcatcg ggctggtatg ggtgcgcagc
      781 agcaaaggct tgctatcacc accttcgatg tagaaggtat cctgcatcga acgcgccgga
      841 tgattttccg gactgttcag tgcagagaaa ttggtccagt cagtttctat ctcggggcca
      901 tcggcaacgt cgaagccgat cgagcggaag atttcttcaa cgcgttgcca cgagcgcatg
      961 accggatgaa tgccgccttt gccgcggccg cgacccggca aggtcacatc gatggcttcc
     1021 gcatcaaggc gctcctgcat ttgcgcattg gcgagggcat cgcgacgcgc aaccaacgcg
     1081 ctctcgatct gctccttgac agcattgatg acggcgccct gcgctttgcg atcttccggc
     1141 gccagcttgc ccaaccgctt catctgctcg gtgatctgac cggttttgcc aaaatacctg
     1201 gctttggcat tttccagggc ggcggcatca ggtacgccag cgaagtcggc ccgggcctgt
     1261 gcgacgagtt gttccaggga attcatgcgg taagcctcgt tccattcaga tagcatcatc
     1321 cgggaacgcg ttcacgttcc acaacataaa tggatacggg gcataggtgc ttcacctctg
     1381 ccccgcaaga tttgcagcac cgcccagaat cgggcgacga ctgcatcgac acggcgaatt
     1441 acctagcgat gccggctttt acctgattga caatcgcagc gaacgccggc ttgtccgtca
     1501 ccgccatatc ggcaagaacc ttacggtcca gttcgatcga ggcgcgcttg aggccattca
     1561 tgaacacgct gtaggtcacg ccatgctcac ggacagcggc attgatacgg gtgatccaca
     1621 atgcgcggaa tacgcgcttt ttgttgcggc gatcacggta agcgtattgg ccggcacgca
     1681 tgactgcctg cttggcaata cgataaacgg tactgcggcg cccccggtaa cccttggctt
     1741 gtgccaggac ttttttatga cgggcacgtg ctgtcacccc acgtttgact ctaggcatgg
     1801 tagctcctta atgtgagttc aagcgaacgg caacattgcg cgaaccgatg tcatgtcggc
     1861 ctcatggact cccactgcac cgcgcaattg acgtttgttc ttggtggtct tcttggtcag
     1921 tatgtgacgt ttgaacgcct gaccgcgttt gacggtacca cccggacgga cgcggaagcg
     1981 ctttttggcg ctgcttttcg tcttcatttt tggcatgaca ctatctaccc ttttcgggac
     2041 agctccattt ttgatgatga caggtggcag caatacgctg ctcttggatg cctgctctca
     2101 cttatcggca acagtttcca ctgctgcttt cgcgggaaga aaaacgatcg cttcccgaca
     2161 aaatcttcgg tatcgctaca gctatcgctt gccgtaccgg aatacacatc gcaacaagct
     2221 gcctgcgatg cgcaaaaata tggcgtactt ttttatttct tctttttcgg cgccagaacc
     2281 atgatcatct ggcgcccttc catcttcgga aactgctcga cctgaccatg tggcgccaga
     2341 tcggtcctca aacgctccag cacgcgcatg ccgatttcct ggtgggccat ttcacgacca
     2401 cgaaaacgca gcgtgatctt ggtcttgtcg ccatcttcca ggaatctggt cagattacgc
     2461 agcttgatgt tgtaatcgcc atcatccgta ccaggacgga atttgacctc cttgacctga
     2521 atgatctttt gcttcagctt ggcttcgtgc gccttctttt gttcctgata tttaaacttg
     2581 ccataatcca tcaaacgcgc gacagggggc tgagcagttg gtgcaatttc caccaaatcc
     2641 acattcgctt cttccgccag acggaatgcc tctgccaaat tgacgatacc gagcggctcg
     2701 ttatcgactc cggataaacg caattccggt gcattgattt caccgttgat gcggtgcgac
     2761 ttgtcagtag ctattgcagt ttccttttaa cgtccaatga atcaagccgt gctgttgcgc
     2821 tgtaaggagc tttactcccc taggctttgg cttccacctc gctcttgagg cgttccacca
     2881 aaacatcgac aggcataaca cccaaatcga cattgccccg cgcacgcacg gccactgtgt
     2941 ttgtatcacg ttctttatcg ccaactacaa gaatataggg cggcttctgt agagaatgct
     3001 gccgtatttt ataagtaatt ttctcgttac gcaaatccgt cacgactcta aacccttgtt
     3061 ttttcagagt ttgcgcaagg tccatgacat attcggactg cgcgtcggaa atattcagaa
     3121 tgacagcttg ctctggagca agccacaaag gcatcgcacc agcatagttt tcaatcagaa
     3181 tagcgatgaa acgctccaac gaaccaacga tcgcgcggtg cagcatgacc ggcgtcttgc
     3241 gctcattgtc ttcagtgaca tattccgccc ccaagcgcgc cggcatcgag aaatccacct
     3301 gcatggtacc gcactgccag gaacgaccga tcgaatcctt caggtggtat tcgatcttcg
     3361 gaccatagaa cgcgccctca cccggcagtt cctcccatcc tgtaccggaa gcgcgaatcg
     3421 cttcgcgcaa ggcgttctcc gccttattcc ataccgcctc ttcgccgatg cgcttttccg
     3481 gacgcagggc taacttcaca gtcacatcgg tgaaaccaaa agttgcatag acctcgcgca
     3541 ctatcctgtc gaaggctgcg acctctccct gcacctgatc ttcagtacaa aaaatatggc
     3601 cgtcatcctg ggtaaaacca cgcacgcgca tcatgccgtg caacgcgccg gatggttcat
     3661 tgcgatggca ttgaccaaat tcgccgtagc gcagcggcag atcacggtag ctgtgcagat
     3721 cggagttgaa aatctgaatg tggcccgggc agttcatcgg tttcag
//
LOCUS       DASRDH010000262         2172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_771493,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000262 DASRDH010000000
VERSION     DASRDH010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_771493"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(290..850)
                     /locus_tag="VFA14_06170"
     CDS             complement(290..850)
                     /locus_tag="VFA14_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006390368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HZS80993.1"
                     /translation="MLRRNPSGDFPQVHESTYVDQTAILCGKIIVHENVFIGPYAVIR
                     ADEIDANGRMAPIIIHADSNIQDGVVIHSKSGGAVTIGERTSIAHRAIVHGPCTVGHD
                     AFIGFNSVLFNCVIGDGCVVRYNAVVDGCNLPPGFHVPSTLRIGPQTNLEALPRVCAQ
                     TSEFSESVARTNNELVQAYKKLQNEF"
     gene            complement(949..1539)
                     /locus_tag="VFA14_06175"
     CDS             complement(949..1539)
                     /locus_tag="VFA14_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008326823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HZS80994.1"
                     /translation="MPTYTLATAVEVETEVRKSRFIGIVMPVDSRDAAQSELERLRAR
                     YSTASHLCWALMAGGQSGMSDDGEPSGTAGRPMLEVLRHHELDGVLAATVRYYGGIKL
                     GAGGLARAYTDAIAAALKQARRIECIAYAEVEIAIAYADESHLRHWLRQKQYALLGSS
                     HDTLARLQVRLPVADFTQLTASMPRLTAPAIIRRRS"
     gene            complement(1844..>2172)
                     /locus_tag="VFA14_06180"
     CDS             complement(1844..>2172)
                     /locus_tag="VFA14_06180"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015004074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HZS80995.1"
                     /translation="CSMAAGALAAAMGGTPAQVENAAEIGMEHNLGLTCDPVGGMVQI
                     PCIERNAMGAVKAINAARMALRGDGSHYVSLDTVIKTMMQTGEDMKSTYKETSLGGLA
                     VNVVEC"
BASE COUNT          448 a          599 c          640 g          485 t
ORIGIN      
        1 tcaaaacctc gaatggtatg tcctgccgca gccgctgact ctgtcggcca tgcagatagc
       61 cgatttccat cgctactaca cacgtaacaa tcgtgatcta cagcctcgga atgatcgcga
      121 acttctgtat tttgccgaca aatgaactgt gtctgattcc aggcaggctc ctgagaacct
      181 gtttacccta tcaacctgac tgcaccttgc atcccaacct gaaaaaacgg tcggacgata
      241 atccggttct tctgcgcagg agaaccggat ttttcatagg tgcgccgtat cagaattcat
      301 tttgcagctt cttgtaggcc tgcaccagtt cgttattggt acgggcgacg ctttcggaga
      361 attccgatgt ctgcgcgcac acgcgcggca gcgcttcgag attggtttgc ggtccgatgc
      421 gcagtgtcga cggtacgtga aagccgggcg gcagattgca gccgtccacc acggcgttgt
      481 aacgcactac gcaaccgtca ccgatgacgc agttgaacag cacgctgttg aagccgatga
      541 aggcgtcgtg tccgactgtg caagggccgt ggacaatggc gcggtgagcg atcgaggtac
      601 gctcaccgat ggtcacggcg ccgccggact tggaatggat cacgacacca tcctgaatgt
      661 tggaatcggc gtggataatg atgggagcca tcctgccatt cgcatcgatc tcatcggcgc
      721 ggatgacagc gtaggggccg atgaagacat tttcatgcac gatgattttg ccgcacagga
      781 tggctgtctg gtcgacatag gtggattcgt gtacctgggg aaagtctccg gaaggattgc
      841 gtcttagcat aagttacgat ctccttgaac agatagggtg gtgaggtgct gaatgatacc
      901 ctgttcgaca tatcttgccg gtcaggatgg attagagccg ctaggttctt agctgcgccg
      961 tctgatgatg gcgggtgcag tcagtcgcgg catggatgct gtcaactgcg tgaaatcggc
     1021 gacgggcagc cgtacctgca gccgtgccag cgtgtcgtgg ctgctgccga gcagggcgta
     1081 ctgcttttgc cgcagccagt ggcgcagatg agattcatcg gcataggcga tggcgatttc
     1141 cacttcggcg taggcgatgc attcgatgcg ccgcgcctgt ttcaacgccg ccgctatggc
     1201 atcggtatag gcgcgcgcca gtccgcccgc tcccagcttg ataccgccgt agtagcgcac
     1261 cgtcgccgcc agtacgccat ccagttcgtg atggcgcaaa acttccagca tcggacgtcc
     1321 agctgtgccg gagggttcgc cgtcgtccga catgccggat tgaccaccgg ccatcaaggc
     1381 ccagcatagg tgcgaagccg tgctgtatcg tgcgcgcagg cgttccagct cggactgcgc
     1441 ggcatcgcgg ctgtcaaccg gcatgacgat gccgatgaag cggcttttgc ggacttcggt
     1501 ttcaacctcg acggcggtag caagggtata agttggcaag atacataggc gctaccggag
     1561 atcaggcaaa ccagtcaacc agcaagctgc gctcaagaat ggcacatccg taggcggtga
     1621 gattaatgtg ttgtagaagt gcgcgtgata tttatggtat tcccaatttg cttcggtgag
     1681 tcggagacga accggaactt tacattattg gcaactgtaa ttttaagttg ttgcccataa
     1741 ggatcttgag attgaggtca agaccaccga tgctggaaag cttgggacat tgctcgtcag
     1801 taaaggcaat atcggatggc tggcagttac cttggtttat gagtcagcac tccaccacat
     1861 tgaccgcgag gccaccaagc gatgtttcct tgtacgtcga cttcatatct tcaccggtct
     1921 gcatcatggt cttgatgacc gtatcgagtg agacatagtg gctgccatcg ccgcgcagcg
     1981 ccatgcgcgc cgcgttgatc gccttgaccg cgcccatcgc gttgcgttcg atgcagggaa
     2041 tctgcaccat gccgccgacc gggtcgcagg taaggcccag gttatgctcc atgccgatct
     2101 cagccgcgtt ctcgacttgc gccggcgtac cgcccatcgc agcggccagc gcgccggccg
     2161 ccatcgaaca gg
//
LOCUS       DASRDH010000263         4513 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_975157,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000263 DASRDH010000000
VERSION     DASRDH010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 4513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4513
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_975157"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..622)
                     /gene="eutC"
                     /locus_tag="VFA14_06185"
     CDS             complement(<1..622)
                     /gene="eutC"
                     /locus_tag="VFA14_06185"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008851 - ethanolamine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HZS80996.1"
                     /translation="MDEIKKRDKSSKPGGAGKPVTDNPWAALRRFTDARIALGRAGVS
                     LPTQAQLSFQLAHAQARDAVHLALDVDALKAQLQKHGIGAAGEAMQLHSAAPDRLTYL
                     QRPDLGRRLSNESRAQMLAAPFAAATQARPYDVAFVIADGLSSLAVGQNAAPFLAALT
                     RRIASENWRIAPPVIVRQGRVAVADEVGELLGAKLVVILIGERPGLS"
     gene            complement(626..2032)
                     /locus_tag="VFA14_06190"
     CDS             complement(626..2032)
                     /locus_tag="VFA14_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HZS80997.1"
                     /translation="MPEHRFKHSVGSASHVFRDLKELMAKASPARSGDMLAGVAAATA
                     EERVVAQMVLAGLPLKTFLEEALVPYEKDEITRLIVDTHDRAAFAPIAHLTVGDFRNW
                     LLTDDTDGAALAAVAPGITPEMAAAVSKIMRNQDLILVGKKCCVVSAFRNTIGMPGRL
                     STRLQPNHPTDDVSGIAASMLDGLLYGNGDAVIGINPATDNVPQVMRLVGMMADVIAR
                     YEIPTQSCVLTHVTNTIAAIERGAPVDLVFQSIAGTEAANRGFGIDLAILGEAREAAL
                     SLKRGAVGDNVMYFETGQGSALSANAHHGIDQQTCETRAYAVARAFKPLLVNTVVGFI
                     GPEYLYDGKQIIRAGLEDHFCAKLLGLPMGCDVCYTNHAEADQNDMDVLLTLLGVAGC
                     TFVMGIPGSDDIMLNYQTTSFHDALYARRVLGSRPAPEFEDWLRRMQIFNAGEGETQF
                     RLHAGTPPGFRDALLRLH"
     gene            2170..3372
                     /locus_tag="VFA14_06195"
     CDS             2170..3372
                     /locus_tag="VFA14_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HZS80998.1"
                     /translation="MHDIICPHCGKAFKVDGAGYANILKQVRDSEFDKQLHERLALAE
                     QDKWNAVELARTKVAGDMQKIAAARDAEIQELRAKLDAGEIARKLAVAEAVNMVEKER
                     DELKNGLARAALEKQLAEQSLKDKYETQIKDRDDAIERLRDMKARLSTKMVGETLEQH
                     CETEFNRIRATAFPRAYFEKDNDARTGSKGDYLFRDADEAGTEIVSIMFEMKNESDRT
                     ATKNRNEDFLKELDKDRTEKRCEYAVLVSLLEPDSELYNTGIVDVFHRYPKMYVVRPQ
                     FFIPIITLLRNAAMHSLKYKSELALVKAQNIDITNFETELETFKSAFAKNYDLASRRF
                     QTAIDEIDKSIEHLQKTKEALLGTDRNLRLANDKAQDVTLKKLTRGNPTMAARFAELG
                     DHGPSKDD"
     gene            complement(3447..4505)
                     /locus_tag="VFA14_06200"
     CDS             complement(3447..4505)
                     /locus_tag="VFA14_06200"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006477484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HZS80999.1"
                     /translation="MLTSHSCGYAAKTAQSALAPYTFERREPGAHDVRIDIMYCGVCH
                     SDLHTARNEWQNTIYPVVPGHEIIGRVSKVGSHVRQFRSGQMVGVGCMVDSCQSCASC
                     AEGLEQYCENGFVGTYNSEEKHIGGMTYGGYANNIVVNEKFVLRVSDKLDPAAAAPLL
                     CAGITTYSPLRHWKVGKGHKVGIVGLGGLGHMGVKIAHAMGARVVLFTTSPDKQQDAL
                     KLGADEVVISTDPAQMAQHQNSFDFILDTVAAPHDLDAFLVLLKREGTMTLVGAPATP
                     HPSPQAFNLIFKRRRLAGSLIGGIKETQEMLDFCAEHGITSDIELIRIQDINTAYERM
                     LKSDVKYRFVIDMASLAT"
BASE COUNT          942 a         1369 c         1393 g          809 t
ORIGIN      
        1 agctcaggcc aggacgctca ccgatcagga tcaccaccag cttggcgccc aacagctcgc
       61 cgacttcgtc ggccaccgcg acgcgcccct ggcgcacgat gaccggcggc gcgatacgcc
      121 agttctccga cgcgatgcgg cgcgtaagcg cggccaggaa aggcgcggcg ttctgtccca
      181 ctgccagcga cgacaggccg tcggcgatga cgaaggcgac atcataaggc cgcgcctgcg
      241 tcgccgcggc gaacggtgcg gccagcatct gcgcgcgcga ttcattggac aagcgccgcc
      301 ccagatcggg acgctgcagg taggtgagcc ggtcgggcgc agcgctatgc agctgcattg
      361 cctcgccggc tgcgcctatg ccgtgcttct gcagctgcgc cttcagcgca tctacgtcca
      421 gcgccagatg taccgcgtcg cgcgcctgcg cgtgcgccag ctggaaggac agctgtgcct
      481 gcgtaggcag gctcacgccg gcgcggccca aggcaatgcg cgcgtcggtg aagcggcgca
      541 gggctgccca gggattgtca gtcaccggct tgccagcacc gccgggcttg ctgctcttgt
      601 ctctcttttt gatctcgtcc atgggtcagt gcagcctcag caaggcgtcg cggaaacccg
      661 gcggcgtgcc ggcatgcagg cggaactgcg tctcgccctc gccagcgttg aagatctgca
      721 tccgccgcag ccagtcctcg aactccggcg ccggacgcga acccagcacg cgacgcgcgt
      781 atagcgcgtc gtggaaggat gtagtctggt agttgagcat gatgtcgtcc gagccgggaa
      841 tgcccatcac gaaggtgcag ccagccaccc ccaggagcgt gagcagcacg tccatatcgt
      901 tctggtcggc ctcggcgtgg ttggtgtagc agacgtcgca gcccatcggc aggcccagca
      961 gcttggcgca gaaatggtct tccaggccgg cgcggatgat ctgcttgccg tcataaagat
     1021 attcggggcc gatgaaaccg accactgtat tgaccagcaa cggcttgaat gcgcgcgcca
     1081 ctgcgtaggc gcgcgtctcg caggtctgct ggtcgatgcc gtggtgggcg ttggccgaga
     1141 gcgcgctacc ttggccggtt tcaaaataca tgacgttgtc acccacggcg ccgcgtttca
     1201 atgacagcgc cgcctcgcgt gcctcgccaa gaatagcgag gtcgatgccg aagccgcggt
     1261 tggcggcctc ggtgccggcg atggactgga acaccaggtc caccggcgcg ccgcgctcaa
     1321 tggcggcgat cgtgttggtg acgtgggtca gcacgcagga ttgggtggga atctcgtagc
     1381 gggcaatgac atcggccatc atgccaacca gtctcatcac ctgcggcaca ttgtcggtgg
     1441 cgggattgat gccgatcacg gcgtcgccgt tgccgtacag caggccgtcg agcatgctgg
     1501 cagcgatgcc gctgacgtcg tcggttggat ggttgggctg caatcgcgta gatagccgcc
     1561 ctggcatgcc gatggtgttg cgaaaggcgc tgaccacgca gcattttttg ccgaccagga
     1621 tcagatcctg attgcgcatg atcttggaca ccgccgccgc catctccggc gtaatacccg
     1681 gcgcaacggc cgccagcgca gcgccgtcgg tgtcgtcggt cagtaaccag ttgcgaaagt
     1741 cgcccaccgt caggtgcgcg atcggcgcga aggcggcgcg gtcgtgcgtg tcgacgatca
     1801 gacgtgtgat ctcgtccttc tcatagggaa ccagcgcctc ctccaggaat gtcttgagcg
     1861 gcagcccagc cagcaccatc tgggctacca cgcgctcctc ggcggtggcc gctgccaccc
     1921 cggccagcat gtcgcccgag cgtgccggcg aagccttggc catcaactcc ttcaggtcgc
     1981 gaaaaacatg gctggcgctg ccaacgctgt gcttgaaccg atgttccggc atggtcatcc
     2041 tccctcttca acgcagccgc gccgctacgc tccaggcgca ggccgatgat gttcccgcgt
     2101 ggcacattgt agcccttcct gtagactact ggatccacag aaattgagtg agatcaggag
     2161 cgcatagaca tgcatgacat catctgcccg cactgcggga aggcattcaa ggtcgacggg
     2221 gccggttacg cgaacatcct gaagcaggtt cgcgacagcg agttcgacaa gcagttgcat
     2281 gagcggcttg cgctggccga gcaggacaag tggaacgccg tcgagcttgc ccggacgaag
     2341 gtcgccggcg acatgcagaa aattgccgcg gccagggatg ccgaaatcca ggaactgaga
     2401 gcgaaactcg acgccggtga gatcgcgcga aaacttgcgg tcgccgaggc agtcaacatg
     2461 gtcgagaaag aacgcgatga actgaaaaac ggcctcgcgc gagcagcgct cgagaagcag
     2521 ctcgcggaac agtcgctcaa ggacaagtac gagacgcaga tcaaggatcg cgatgacgcg
     2581 atcgaacgcc tgcgggacat gaaggcccgc ctgtcgacca agatggtcgg cgaaacccta
     2641 gaacagcatt gcgagactga attcaaccgt attcgggcga cggcgtttcc aagggcctat
     2701 ttcgagaagg acaatgacgc gcggaccggc agcaaaggcg actacctctt ccgcgacgcg
     2761 gatgaggcag gtacggagat cgtctctatc atgttcgaaa tgaagaatga gagcgaccgt
     2821 accgcgacga agaacagaaa cgaggacttc ctgaaggaac tcgacaagga tcgcaccgag
     2881 aagagatgcg agtatgcggt gctggtctcc ctgctcgaac ccgacagcga actctacaac
     2941 accggcatcg tcgatgtgtt ccaccgctac ccgaagatgt atgtcgtccg gccacagttc
     3001 ttcatcccta tcattacgct gctgcgcaac gcggcgatgc actcgctcaa atacaaatca
     3061 gagctggcac tcgtgaaggc gcagaacatc gacatcacaa atttcgaaac agaactagag
     3121 actttcaaga gcgcattcgc aaaaaactac gatctcgcct ccaggcgctt ccagacggcg
     3181 atcgatgaga tcgataagtc gatcgagcac ctgcagaaaa cgaaagaggc cctgctcggc
     3241 accgaccgga acctgcgtct tgcgaacgac aaggcgcagg acgtgacgct caagaagctg
     3301 acccggggca acccgacgat ggcggccagg ttcgccgaac tcggggatca cggtccttcc
     3361 aaggatgatt gagtcagtcg gcaagttaac agatcttttg gaaacaactt ccaagaacct
     3421 gttgacgatc tcatcgggaa caggttctaa gtcgccagcg aagccatatc gatcacaaag
     3481 cggtatttga catcgctttt gagcatgcgc tcgtaagccg tgttgatgtc ctgaatcctg
     3541 atcagctcga tgtcggaggt gatgccatgc tcggcgcaga agtccagcat ttcctgcgtt
     3601 tccttgatgc cgccgatcag cgagccggcc aggcgacggc gtttgaagat caggttgaac
     3661 gcttgcggcg aaggatgcgg cgtggccggc gccccgacca gtgtcatggt gccttcgcgc
     3721 ttgagcagca ccaggaaagc atcaagatca tgcggcgccg ccacggtatc aagaatgaag
     3781 tcgaagctgt tctgatgctg cgccatctgc gccggatctg tggaaatgac gacttcatcg
     3841 gcgcccagct tcaaggcatc ttgctgcttg tccggcgatg tcgtgaagag caccacgcgc
     3901 gcgcccatcg catgcgcaat cttgacgccc atatgaccga ggccgcccag cccgacgatg
     3961 ccgaccttgt gccccttgcc gaccttccag tgacgcagcg gtgaataagt cgtgatgcca
     4021 gcgcacagca agggcgcggc ggcggcagga tcgagtttgt cggacacgcg caaaacgaac
     4081 ttctcattga ccacgatatt attggcataa ccgccgtagg tcatgccgcc gatatgcttt
     4141 tcttcactgt tgtaagtgcc gacgaaaccg ttctcgcaat actgctccag accctcggcg
     4201 caactggcgc aagattggca ggaatcgacc atgcagccga cgccgaccat ctggcctgac
     4261 ctgaattgcc tcacatggga gccgaccttg ctgacgcggc caatgatttc gtgaccgggt
     4321 acaaccgggt aaatcgtgtt ctgccattcg ttgcgggcgg tgtgcaggtc ggaatggcaa
     4381 acgccgcaat acatgatgtc gatccgtaca tcatgcgcgc cgggttcgcg tcgttcaaac
     4441 gtgtaaggcg ccagtgcgga ttgtgcggtt tttgcggcat aaccgcagga atgagaggtc
     4501 agcatgatga atc
//
LOCUS       DASRDH010000264         2757 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_975280,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000264 DASRDH010000000
VERSION     DASRDH010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_975280"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /locus_tag="VFA14_06205"
     CDS             complement(<1..661)
                     /locus_tag="VFA14_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006758807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZS81000.1"
                     /translation="MSTPELASSEHSWWGVLALALSAFIFNTTEFVPVALLNAIGNSF
                     HMQPTDVGLMLTIYAWTVAVVSLPLTLLTRTVERRKLMMGVFALFIAGHVVTGVAWSF
                     PVLVIGRTGIACAHAIFWSISVSLVVRLAPPEKKSRALGLLAMGTSIAMVAGIPLGRV
                     VGEAMGWRTTFLTIACTAGIALLMLRNTLPNLPNQQAGSFSSLPMLLKKPALASLYLI
                     TI"
     gene            complement(787..2253)
                     /gene="rng"
                     /locus_tag="VFA14_06210"
     CDS             complement(787..2253)
                     /gene="rng"
                     /locus_tag="VFA14_06210"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HZS81001.1"
                     /translation="MNEEDILINITPQETRVALILQGIVQELHIERTASRGLTGNIYF
                     GKVVRVLPGMQSAFIDIGLERTAFLHITDIREARPHHDRNASPMPIEKLLFDGQSITV
                     QVIKDPIGAKGARLSTQISIAGRMLVYLPQDRHIGISQRIGNETERELLLSRVQKLLP
                     PEEKSGFIIRTMAEDASDTDLRMDIDYLRKIWASIVAQTGTRPAPALMYQDLNLAQRV
                     LRDFVSDDTSSIQIDSHENFQMLKEFGAMYTPSVLPRLQHYRGERPLFDLYSVEEEIE
                     RALGRRVDLKSGGYLIIDQTEAMTTIDVNTGSFVNGYNFDDTIFKTNLEATHAIARQL
                     RLRNLGGIIILDFIDMGNLEHRNVVLAELNKALQRDRTKVSVSGFSTLGLVEMTRKRT
                     RESLAHILCETCPACKGQGQVKTTRTVCYEILREVLREAKQFNPREFRILAAQMVIDM
                     FLEEESQHLAMLGDFIKKPVSLQVQADYQQEQYDIVLM"
     gene            complement(2301..>2757)
                     /locus_tag="VFA14_06215"
     CDS             complement(2301..>2757)
                     /locus_tag="VFA14_06215"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HZS81002.1"
                     /translation="ARDKLISARRTMLCRQLPMRPILAADTTVVIDDCILGKPADTGA
                     AIDMLTRLSGRTHQVLTSVAVAFQEQTWQITQASHVVFATLTDNMIRAYCTTPEPYDK
                     AGGYGIQGLAAMFIENIAGSHSGIMGLPLFETAQLLQKTGISILPPATW"
BASE COUNT          534 a          723 c          860 g          640 t
ORIGIN      
        1 ggatcgtgat caggtatagt gatgccagcg ccggtttttt caagagcata ggaagactgc
       61 tgaacgaacc cgcttgctgg ttcgggagat taggcaaggt atttcgtagc atcagtagcg
      121 cgattcccgc cgtacatgcg atagtcagga acgttgtccg ccagcccatc gcttcgccga
      181 cgacgcgccc gagaggaatg ccggccacca ttgcgatcga tgtacccatc gcgagcagac
      241 cgagcgcccg gcttttcttt tcgggcggcg ccagccgcac aaccagcgaa actgagatcg
      301 accagaagat cgcgtgtgcg cacgcaatgc ctgttctgcc gatgaccagc acgggaaaac
      361 tccacgcgac gccggtcacg acatggccgg cgatgaacag tgcgaacacc cccatcatta
      421 gcttgcggcg ttcgacggtt cgagtgagaa gcgtcaacgg gagagacacg acggctacgg
      481 tccatgcata aatcgtgagc atgagaccga catcggtcgg ttgcatatga aagctgttgc
      541 caattgcgtt gagcagtgcg acgggtacaa attcagtcgt gttgaagatg aaggcggaaa
      601 gcgccaatgc cagcactccc caccaggagt gttcggagga agccagttca ggagttgaca
      661 tacctattga tttttacatg tcctagctat gacttgatct gacagttacc gacgtcattt
      721 tgcagttgaa acgagatcag acgtcgggtc tgtccttgac ttgtgtatga cgatatgggg
      781 taaaccctac atcaatacga tgtcgtactg ttcctgctga taatccgcct gcacctgcaa
      841 tgaaaccggt ttcttgatga agtcgcccag catggccagg tgttgcgatt cttcttccaa
      901 gaacatgtcg atgaccatct gtgctgccag gatgcggaat tcgcgcgggt tgaactgttt
      961 ggcttcgcgc agtacttcgc gcaggatttc gtagcacacc gtgcgcgtgg tcttgacctg
     1021 gccctggcct ttgcaggcgg ggcaggtttc gcacaggata tgcgccagcg attcgcgtgt
     1081 gcgtttgcgt gtcatttcta ccaagcccag cgtcgagaag ccggagacgg aaaccttggt
     1141 gcggtcgcgt tgcagcgctt tgttcagttc ggcgagtaca acgttgcggt gctccaggtt
     1201 tcccatgtcg atgaaatcga gaatgatgat gccgcccaga ttgcgcaggc gtagttggcg
     1261 cgcgatcgca tgcgttgctt ccagattggt tttgaagatg gtgtcgtcga agttgtagcc
     1321 gttgacgaaa ctgccggtgt tgacgtcgat ggtggtcatc gcttcggtct ggtcgatgat
     1381 gaggtagccg ccggacttca gatcgacgcg gcgtcccagt gcgcgttcga tttcttcttc
     1441 gacgctgtac aggtcgaaca gcggccgttc gccgcggtaa tgctgcagcc tgggcagtac
     1501 cgagggtgta tacatcgcgc cgaactcttt cagcatctga aaattctcgt gcgagtcgat
     1561 ttgaatgctg gaagtatcgt cgctgacgaa atcgcgcagc acacgctggg ccagattcag
     1621 atcctggtac atcagcgccg gcgcaggtct ggttccggtc tgggcgacga tgctggccca
     1681 gattttgcgc agatagtcga tgtccatccg taggtcggtg tcggaggcgt cttcagccat
     1741 ggtgcggatg atgaagccgc ttttttcttc aggcggcagc agcttctgta ccctgctgag
     1801 cagcagttcg cgttcggttt cgttgccgat gcgctgcgaa ataccaatgt gtctgtcctg
     1861 cggcaggtag accagcatac gcccggcgat ggagatttgc gtcgacaggc gcgcgccctt
     1921 ggcgccgatc ggatccttga tgacctgtac tgtgatggac tgaccgtcga acagcagctt
     1981 ttcgatcggc atcggtgagg cattccggtc atgatgcggt ctggcttccc ggatgtcggt
     2041 gatatgcaga aaggcggtgc gttccaagcc gatgtcgatg aaggccgact gcatgccggg
     2101 cagcacgcgc actactttgc cgaagtaaat attgccggtc aggccgcgcg atgccgtgcg
     2161 ttcgatgtgc aattcctgca ctatgccctg caggatcagt gcgacgcgcg tttcctgtgg
     2221 cgtgatgttg atcaggatgt cttcttcgtt catgtctgga agtgtgcatg ccggcagaga
     2281 gccacaggat ggatcgagac tcaccatgtt gccggcggca ggatgcttat gccagttttt
     2341 tgcagcagtt gcgctgtctc gaacagcggc aggcccatga tgccggaatg actgccggcg
     2401 atgttctcga tgaacatcgc agccagtccc tggatgccat agccgccggc cttgtcgtaa
     2461 ggttctggtg tggtgcaata ggcgcggatc atgttgtcag tcagcgttgc gaaaacgaca
     2521 tgggaggctt gcgtgatctg ccatgtctgt tcctggaatg ccacggcgac actggttagc
     2581 acttggtgcg tgcggcccga caggcgcgtg agcatgtcga tggctgctcc ggtgtcagcg
     2641 ggtttgccga ggatgcagtc gtcgatgacg accgtggtgt cggccgccag tatgggacgc
     2701 atcggcagtt gccggcagag catggtccgg cgtgcgctga tgagcttgtc gcgcgcg
//
LOCUS       DASRDH010000265        10511 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_431068,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000265 DASRDH010000000
VERSION     DASRDH010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 10511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10511
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431068"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            299..1348
                     /gene="dusB"
                     /locus_tag="VFA14_06220"
     CDS             299..1348
                     /gene="dusB"
                     /locus_tag="VFA14_06220"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HZS81003.1"
                     /translation="MNIGPHFLRNKVFVAPMAGVTDRPFRQLCKQLGAGYAVSEMAAS
                     NPRLWRSEKTSRRTSHDGEMEPKAVQIAGADAAMLADCARYNVDRGAQIIDINMGCPA
                     RKVCNSWCGSALLQDEKLVGRILDAVVKAVDVPVTLKFRTGWNRANRNALTIAAMAEQ
                     SGIAMLTLHGRTRADGYSGAAEYQTIAAVKAAVKIPVVANGDITTPERAQHVLAVTGA
                     DAVMVGRAAQGRPWIFRGIDHFLRTGTHLPAPLVVEARDLMLEHLQAHYAFYGEYLGV
                     RTARKHIGWYVRDLPGGAAFRQRMNRLEDCRLQWQAVHDFFSSQSAHGERLQCGFAGR
                     AGGVVTAGLPAIDQH"
     gene            1358..1591
                     /locus_tag="VFA14_06225"
     CDS             1358..1591
                     /locus_tag="VFA14_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013090496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HZS81004.1"
                     /translation="MSKESIEDIVRKSLDKYFKDLGEQLPSNVYDMVVLMVEKPTFEV
                     VMARADGNQSQAAEILGINRNTLRKKLQQHGLL"
     gene            1681..3252
                     /gene="purH"
                     /locus_tag="VFA14_06230"
     CDS             1681..3252
                     /gene="purH"
                     /locus_tag="VFA14_06230"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HZS81005.1"
                     /translation="MIKQALISVSDKAGVLDFARALSVMGVNILSTGGTARLLADSGV
                     KVTEVADYTGFPEMLDGRVKTLHPKVHGGILARRDFSGHVAALDQHGIPAIDMVVVNL
                     YPFQQTVAKVECSLEDAIENIDIGGPAMLRSAAKNHKDVIVLCDPADYAGVLADLQAG
                     GDLSCEKRFALAGKVFAHTAQYDGAITNYFTSLGADRQHATRSAYPGILNLHFEKVQD
                     MRYGENPHQSAAFYRDVNRVDGALANYTQLQGKELSFNNIADADAAWECVKTFDAAGT
                     VACVIVKHANPCGVAVGANPLESYSKALQTDPASAFGGIIAFNRELDGAAAEALARQF
                     VEVLIAPSFSAAARQVFAARQNVRLLQISSGNGLNTCDFKRVGGGLLVQSPDARNVLL
                     PEVKVVSRKQPTQQQLQDLMFAWRVAKFVKSNAIVFCGNGMTLGIGAGQMSRIDSARI
                     AAIKAQNAGLTLTGSAVASDAFFPFRDGLDVVVDAGAACVIHPGGSMRDQEVIDAANE
                     RDVVMLLTGTRHFRH"
     gene            3282..3821
                     /gene="ruvC"
                     /locus_tag="VFA14_06235"
     CDS             3282..3821
                     /gene="ruvC"
                     /locus_tag="VFA14_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012401929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HZS81006.1"
                     /translation="MKILGIDPGLRTTGFGVIHKHGVRLSYIASGIIKTPDADLPQRL
                     KAILSGVTEIIQTYRPDDAAIEKVFVNVNPQSTLLLGQARGAAICALVNADLPVAEYT
                     ALQVKQAVAGHGKAHKQQVQEMVRRLLALPGLPGTDAADALGVAICHAHSGEALGALG
                     ALAPGFARKGLRVRGGRLV"
     gene            3957..4535
                     /gene="ruvA"
                     /locus_tag="VFA14_06240"
     CDS             3957..4535
                     /gene="ruvA"
                     /locus_tag="VFA14_06240"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HZS81007.1"
                     /translation="MIGRLSGILLEKNPPHLLVDCNGVGYEVSVPMSTFYHLPGLGEK
                     VVLLTYLVIREDAHALFGFGSVEERHAFRELIRIAGVGARTALSVLSGMSVSDLAQAV
                     TLQEAGRFTRVPGIGKKTAERLLLELKGRLGADPGAVGGAARHDTTADMRNALLALGY
                     SDKEAMLALQQVPADTAVPEGIRLALKALSKA"
     gene            4539..5597
                     /gene="ruvB"
                     /locus_tag="VFA14_06245"
     CDS             4539..5597
                     /gene="ruvB"
                     /locus_tag="VFA14_06245"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008117461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HZS81008.1"
                     /translation="MSIRTDDFSEQRIIAATPASAGEEALERALRPKQLDEYVGQEKA
                     RGQLEIFISAARQRGEALDHTLLFGPPGLGKTTMAHIIAREMGVNLRQTSGPVLERAG
                     DLAALLTNLEANDILFIDEIHRLSPVVEEILYPALEDYQIDIMIGEGPAARSIKLDLQ
                     PFTLVGATTRAGMLTNPLRDRFGIVARLEFYSPSELACIVTRSAGLLHAPIVAEGALE
                     IARRSRGTPRIANRLLRRVRDYAEVKGDGRITQTMADAALVMLDVDPVGFDLMDRKLL
                     EAVLFKFSGGPVGLDNLAAAIGEERDTIEDVLEPYLIQQGYLQRTPRGRVATPAAYSH
                     FGVTAPRTDPNGSLWGDA"
     gene            complement(5625..8567)
                     /locus_tag="VFA14_06250"
     CDS             complement(5625..8567)
                     /locus_tag="VFA14_06250"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008117454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HZS81009.1"
                     /translation="MELAKSFEPAEIEKFWREEWEKRGYFAASTAPEKPSFSIQLPPP
                     NVTGTLHMGHAFNQTIMDGLTRYHRMRGFNTAWIPGTDHAGIATQIVVERQLDAQKIS
                     RHALGRKKFVEKVEEWKKKSGSTITGQMRRMGASTDWEREYFTMDPKMSKTVTEVFVR
                     LYEQGLIYRGKRLVNWDPVLGTAVSDLEVVSEEEDGFLWHIRYPLADGSGHVTVATTR
                     PETLLGDVAVAVDPSDERYAHLVGKKLKLPLTRREIPIIADEYVDKEFGTGCVKITPA
                     HDFNDYAVWQRHKGKDGIPHDIGSIFTRNAKIETNFNQSEYVREKFAKQPQAEETTLI
                     ANFDADARAILESIPDKYAGLDRFEARKQMVADLDEQGLLELVKPHRLMVPRGDRSGA
                     VIEPMLTNQWFVKMSEPAKEGTDPYFPGKSIAEVALEKVANGEIRLIPENWNNTYNQW
                     LDNIQDWCISRQLWWGHQIPAWYDEDNKIYVARTEEEAKVQAGGKPLRRDDDVLDTWF
                     SSALVPFSTLGWPEATPDYQLFLPSSVLVTGFDIIFFWVARMVMMTTHFTGQVPFRTV
                     YVHGLVRDASGQKMSKSKGNTLDPIDLIDGIGVDQLVAKRTAGLMHPRQASAIEKATR
                     REFADGIPAFGTDALRFTFASLATLGRNINFDLGRCEGYRNFCNKLWNAARFVLMNTT
                     GQDCGFDGHVNGAGDQEKLPFSRADRWIVSLLQRTEAEVEKGFADYRFDNIAAAIYRF
                     IWDEYCDWYLEMAKSQIQNGSQAQQRATRRTLLSVLEAVLRLAHPVLPFITEALWQSV
                     APLTGRQLDPAGDSIMRQPYPQAEPDKIDEGAEAWMTQLKALTDACRNLRGEMQLSPA
                     LRVPLVVETASPVSVESFLPYLQTLVKLNDTQVMPQLPASPAPVAIVGDLKLMLKVEI
                     DIAAERERLTKEIARISGEVARADGKLSNTGFVARAPEAVVAQERERLAGFTATLAKL
                     QEQLAKLK"
     gene            8769..9791
                     /locus_tag="VFA14_06255"
     CDS             8769..9791
                     /locus_tag="VFA14_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0032775 - DNA methylation on adenine
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HZS81010.1"
                     /translation="MDATALQRQNRRIERLKLQGVTRSTVLVHEECKPALEALRHHLV
                     DPVKAEALTALVTHLQDKKAPTNVAQVRQLSPFRYPGGKTWLVPEVRRWLMFSKLTPS
                     VFVEPFAGGAMAGLTAAAEGLAEHVLLCELDDDVAAVWATIFDGTDADVRWLCESITG
                     FDVTLDNVREVLASNPKTNKGKAFRTIVKNRMQRGGIMAAGAGLVKSGEAGRGLNSRW
                     YPETLVTRIKRLRTLRDRVIFKQGDAFEAVRKYADDPNAFFFIDPPYTAGGKNAGKRL
                     YTHNEIDHEWLFTIMANVSGSVMLTYDDAPEVREMAERHGFRVEPVPMKNAHHTIIRE
                     LLILKP"
     gene            complement(10116..>10511)
                     /locus_tag="VFA14_06260"
     CDS             complement(10116..>10511)
                     /locus_tag="VFA14_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HZS81011.1"
                     /translation="TLSGNPVAVAAGMATLRIVQQAGFYTRLSIQTRKLTDGLCEAAR
                     AAQLSFSADALGGMFGLYFSPAVPTSYAEVMQGDKNRFKVFFHAMLDAGVYLAPSAFE
                     AGFVSAQHDDAIIAATVDAAHDAFAAAAR"
BASE COUNT         2035 a         3094 c         3162 g         2220 t
ORIGIN      
        1 aatgcatcta tcgctgggcc aggcagaccg gttttgcgca gccgcgcgat ttcgatattt
       61 tcaatgccgg tatcaatcgc ctgcaatggg atggtgaacg tctgcacatc agacagtggg
      121 cggacattag ccatttgtcg ctgcaagtgc tggacgaagt ggactgaaaa cctgttcagg
      181 attcctgcgc gggccgtcca ctcgcgcctt tcaggcgctc tgcctggatt cttgttggga
      241 tgcgggacca taatcgcgtt aaaatgacgc ccttctccag caatcgcagc tccgccccgt
      301 gaatatcggc ccgcattttt tgcgtaacaa ggttttcgtc gccccgatgg ccggggtgac
      361 ggaccgtcct ttccgccagt tgtgcaaaca gctgggcgcg ggctatgccg tttccgaaat
      421 ggccgcatcg aatccgcgcc tgtggcgcag cgagaagact tcgcgtcgta ccagtcatga
      481 tggcgagatg gagccgaagg cggtacagat cgccggcgcc gatgccgcca tgctggccga
      541 ctgtgccaga tacaacgtcg accgcggtgc gcagatcatc gacatcaaca tgggttgccc
      601 ggccagaaaa gtctgcaaca gctggtgcgg ctcggccctg ctgcaagatg aaaagctggt
      661 cggacggatt ctcgacgccg tggtcaaggc cgtcgatgtg ccggtaacac tgaaattccg
      721 taccggctgg aaccgcgcaa acagaaatgc cctgactatc gccgccatgg ccgaacaaag
      781 cggtatcgcc atgctgacgc tgcacggtcg cacgcgcgcc gatggctaca gcggcgcagc
      841 cgagtaccag acgattgccg ccgtcaaggc cgccgtgaag ataccagtgg tcgccaacgg
      901 cgacatcacc acgcccgaac gcgcgcaaca tgtgctggct gtgaccggcg ccgatgccgt
      961 catggtaggt cgcgcggcgc aggggcggcc atggattttc cgcgggatcg accacttcct
     1021 gcgcaccggc acgcatttgc cggcgccgct ggtagtcgag gcgcgggatc tgatgctgga
     1081 acacctgcag gcgcactacg cattttacgg cgagtatctc ggcgtgcgca cggcacgcaa
     1141 gcatatcgga tggtatgtcc gtgatctgcc gggcggcgca gccttccggc agcgtatgaa
     1201 ccgcctcgaa gattgccgtt tgcaatggca agccgtgcat gattttttct cctcgcagtc
     1261 ggctcatggc gagaggttac aatgtggttt tgccggtcgc gccggcggcg tagtcacggc
     1321 cggtttaccg gctatcgatc aacactgaaa gtgcgcaatg agtaaagaaa gtatcgagga
     1381 tatcgtcaga aagagccttg acaaatattt caaggatctg ggagaacaac tgccgtcgaa
     1441 tgtctatgac atggttgttc tgatggtcga gaagccgact ttcgaagtgg tgatggcgcg
     1501 cgctgatggc aatcagtcgc aggcggcgga aattctcggc atcaaccgca ataccttgcg
     1561 caaaaagtta cagcaacacg gcttgctgta attaatcgcc cgcgccggca gcatgcggcg
     1621 cagggagcgc tgccagtctc atcaaagcat ttcttcacct atcttcctag ccactccatc
     1681 atgatcaagc aggcactcat ttccgtttcc gacaaagccg gtgtactcga ctttgcgcgc
     1741 gccttatccg tcatgggcgt caacattctg tccaccggcg gcaccgccag gctgctggct
     1801 gacagcggcg tcaaagtgac tgaagtagcc gactacaccg gtttcccgga aatgctggat
     1861 ggccgtgtca agacgctgca tccgaaggtg catggcggca tcctggcgcg ccgcgatttt
     1921 tccggccacg tcgccgcgct ggatcagcat ggcattccgg ctatcgacat ggtggtggtg
     1981 aacctgtacc catttcagca gactgtcgcc aaggtggaat gctcgctgga agacgccatc
     2041 gaaaacatcg acattggcgg cccggccatg ctgcgctccg ccgccaagaa ccacaaagac
     2101 gtgatcgtcc tgtgcgaccc ggccgactac gctggtgtgc tggccgactt gcaggccggc
     2161 ggcgatctgt cctgtgaaaa acgttttgcg ctggctggca aagtgttcgc gcataccgcg
     2221 caatacgacg gtgccatcac caactatttc acttcactcg gcgcagacag gcagcatgcc
     2281 acgcgcagcg catatccggg aatcctgaac ctgcatttcg aaaaagtgca ggatatgcgt
     2341 tacggcgaaa acccgcatca gtcagccgcc ttctaccgcg atgtcaatcg ggtcgatggc
     2401 gcgctggcca attacacgca gttgcaaggc aaggaactgt cattcaacaa catcgccgat
     2461 gccgatgcgg cctgggaatg cgtcaagact ttcgacgctg ccggcactgt cgcctgcgtc
     2521 atcgtcaagc acgccaatcc atgcggcgtg gcggtcggcg ccaatccgct ggagtcctac
     2581 agcaaagctt tgcagacgga tccggcctcg gctttcggcg gcatcatcgc ctttaatcgt
     2641 gaactcgacg gcgccgccgc cgaagcgctt gccaggcaat tcgtcgaagt gctgatcgcg
     2701 ccgtccttca gcgccgccgc cagacaggtt tttgccgcca ggcaaaacgt ccgcctgctg
     2761 caaatctcgt ctggcaatgg tttgaatacc tgcgacttca agcgcgtcgg cggcggcctg
     2821 ctggtgcagt cgcctgacgc caggaatgtg ctgctgccgg aagtgaaggt cgtcagcaga
     2881 aagcagccga cccagcagca actacaggat ctgatgttcg cctggcgcgt cgccaagttc
     2941 gtcaagtcca acgccatcgt attctgcggc aacggcatga cgctgggcat cggtgctggc
     3001 cagatgagtc gcatcgactc cgcgcgcatt gctgcgatca aggcgcagaa cgccggtttg
     3061 acactgactg gttcggcggt ggcgtcggat gctttcttcc cgtttcgcga cggtctggac
     3121 gtggtagtgg atgcaggcgc tgcctgcgtg atccatccgg gtggctcgat gcgcgatcag
     3181 gaggtgatcg atgcggcgaa cgagcgcgat gtggtcatgc tgctgaccgg cacacgtcat
     3241 ttccgccatt gaacattgcg ccgggcggac acacgctctc catgaaaatc ctcggcatcg
     3301 atcccggcct gcgcaccacc ggctttggcg tgattcacaa gcacggcgtc aggctcagtt
     3361 acatcgcctc cggcatcatc aagacgccgg acgccgatct gccgcagcgg ctcaaagcca
     3421 ttctttccgg cgtcactgaa atcatccaga cttacaggcc cgatgacgca gcgatcgaaa
     3481 aagtcttcgt caacgtcaat ccgcaatcca ccttgctgct cggacaggcg cgcggggcgg
     3541 cgatctgcgc gctcgtgaac gccgatttac cggtggcgga atacactgcc ctgcaggtca
     3601 agcaggctgt tgccggtcac ggcaaggcgc acaagcagca ggtgcaggag atggtgcgcc
     3661 gcttgctggc gctgccgggt ttgccgggca ccgacgcggc cgatgcgctt ggcgtggcga
     3721 tctgccatgc acatagcggc gaggctttgg gtgcgcttgg cgcattggcg ccagggtttg
     3781 ccaggaaggg gctgcgcgtg cgcggcggcc gtttggttta gagccgctaa cacaacgccg
     3841 ctggctgcgt tgcgcctgtt tgccgtatct tcgtactgtc tttgtcggcg cgcgtaacta
     3901 tgttgcaaat atcaaacgag agtatcattc ccccattttc tttctgcctg gccatcatga
     3961 tcggtcgtct ttccggaatc ctgctcgaaa aaaatcctcc gcacttgctg gtcgattgca
     4021 acggcgtcgg ttacgaagtc agcgtgccga tgagtacctt ttaccatctg ccgggactgg
     4081 gcgagaaggt cgtgctgctg acttacctgg tgatccgcga agatgcgcac gcgctgttcg
     4141 gttttggcag cgtcgaagag cgtcacgcct tcagggaatt gatcaggatt gccggcgttg
     4201 gcgcgcgtac cgcactgtcg gtgctatccg gcatgtcggt cagcgatctg gctcaggcgg
     4261 tgacgttgca ggaagccggc cggttcacta gagtgccggg tatcggcaaa aaaaccgccg
     4321 agcgtctgct gctggaactc aagggcaggc tcggcgctga tcctggcgcc gtcggcggcg
     4381 cggcgcgtca cgacacgact gcggatatgc gtaacgcctt gctggcgctc ggttattccg
     4441 acaaggaagc aatgctggca ttgcagcagg tgccggccga tacagcggtg ccggaaggca
     4501 tcaggcttgc actcaaggcc ctgtccaaag cgtaaaccat gagcatccgg accgacgatt
     4561 tttccgaaca gcgcatcatc gccgccacgc cggcttcagc cggtgaagaa gcgctcgaac
     4621 gcgcactgcg gccgaagcag ctggatgaat acgtcggtca ggaaaaagcc cgcggacagc
     4681 ttgaaatttt catctcggcg gcgcgccagc gcggcgaagc gctcgatcac acgctgctgt
     4741 tcgggccgcc gggtctgggc aagaccacca tggcgcacat catcgcgcgc gaaatgggtg
     4801 tcaacctgcg ccagacttcc ggcccggtgc tggaacgcgc cggcgacctc gccgctttgt
     4861 tgaccaatct cgaagccaac gacattctct tcatcgacga gatccatcgt ctgtcgccgg
     4921 tggtcgagga aatcctctac cccgcgctgg aggactatca gatcgacatc atgatcggcg
     4981 aagggccggc cgcgcgcagc atcaagcttg acctgcagcc cttcacgctg gtcggcgcca
     5041 ccacgcgcgc cggcatgctg accaatccgc tgcgcgaccg tttcggcatc gttgcgcgtc
     5101 tggagtttta ctcgccatcc gaactggcgt gcatcgtcac gcgcagcgct ggtctgcttc
     5161 atgcccccat cgtcgcagaa ggcgcgctgg aaatcgccag gcgcagccgc ggcacgccgc
     5221 gtatcgccaa ccgtctgctg cgccgcgtgc gcgactatgc ggaggtcaaa ggcgatggca
     5281 ggattacgca gacgatggct gatgcagcgc tggtgatgct ggatgtcgat ccggtcggtt
     5341 tcgatctgat ggaccgcaaa ctgctggaag cggtgctgtt caaattcagt ggcggcccgg
     5401 tcggcctcga caatctggcc gccgccatcg gcgaagagcg tgacaccatc gaagacgtgc
     5461 tcgagcctta tctgatccag caaggttatc tgcaacgcac gccgcgtggc cgcgtggcga
     5521 cgccggcggc ctatagccac ttcggtgtga ccgcgccgcg caccgatccc aacggcagcc
     5581 tctggggcga tgcctgagga gtaaaagccg ccgctgcggc aggtttactt cagtttcgcc
     5641 agttgttcct gcaactttgc cagtgtcgca gtgaagccgg ccagacgctc cctctcttgc
     5701 gccacgaccg cttccggcgc gcgcgcgacg aaaccggtgt tggacagctt gccgtcggcc
     5761 ctggcgacct cgccgctgat gcgggcgatc tctttggtca ggcgctcacg ttcggcggcg
     5821 atgtcgatct cgaccttcag catcagtttc aggtcaccga cgatggccac cggcgccggc
     5881 gatgccggca gttgcggcat gacctgcgtg tcgttcagtt tcaccagcgt ttgcaggtag
     5941 ggcaggaagg actccactga tacgggactg gcagtctcga ccaccagcgg cacgcgcagc
     6001 gccggtgata actgcatctc gccgcgcagg ttgcggcagg catcggtcaa tgccttcaac
     6061 tgcgtcatcc aggcttcagc gccttcatcg atcttgtccg gctcggcttg cggataaggc
     6121 tggcgcatga tggagtcgcc agccgggtcg agctgcctgc cggtcagcgg cgcgacgctt
     6181 tgccacaatg cttcggtgat gaaaggcagc accggatgcg ccaggcgcag cacggcttcc
     6241 agcacgctca acagcgtgcg gcgggtggcg cgttgctgcg cctggctgcc gttctggatt
     6301 tgcgacttgg ccatttccag ataccagtcg cagtattcgt cccagatgaa ccggtagatc
     6361 gccgcggcaa tgttgtcgaa gcggtagtcg gcaaagcctt tttccacttc cgcttcggtg
     6421 cgttgcagca gcgagacgat ccagcggtcg gctctggaaa atggcagctt ttcctggtcg
     6481 cctgcgccgt tgacatggcc atcgaagccg caatcctggc ctgtggtgtt catcagcacg
     6541 aagcgtgccg cattccacag tttgttgcag aagttgcgat agccttcgca gcggccgagg
     6601 tcgaaattga tgttgcgccc cagcgtcgcc agcgaagcga acgtgaagcg tagcgcatcg
     6661 gtgccgaacg ccgggatgcc gtcggcgaat tcgcggcgcg tggctttctc gatggcggag
     6721 gcttgccggg gatgcatcag gccggcggtg cgcttggcta ccagttgatc cacgccgatg
     6781 ccatcgatca ggtcgatggg atcgagcgtg ttgcccttgg acttggacat cttctggccg
     6841 ctggcgtcgc gcaccagacc atgcacgtag accgttctga acggcacctg gccggtgaag
     6901 tgcgtagtca tcatgaccat gcgcgccacc cagaagaaga tgatatcgaa accggtcacc
     6961 agcaccgacg acggcaggaa cagttggtag tccggcgtgg cttccggcca gcccagcgtc
     7021 gaaaacggca ccagcgccga cgagaaccag gtatccagca cgtcgtcgtc gcgcctcaga
     7081 ggtttgccgc cggcctgcac cttggcttct tcttcggtac gggcaacgta gatcttgttg
     7141 tcttcgtcgt accatgccgg gatctgatgg ccccaccaca actggcgcga gatacaccag
     7201 tcctggatgt tgtcgagcca ctggttgtag gtgttgttcc agttttccgg aatcagcctg
     7261 atctcgccgt tggcgacttt ttccagcgcg acttcggcga tcgacttgcc agggaagtaa
     7321 gggtctgtac cttctttcgc tggctcgctc atcttgacga accattggtt ggtcagcatc
     7381 ggctcgatta ccgcgccgct gcgatcgccg cgcggcacca tcagtctgtg cggtttgacc
     7441 agttccagca gaccttgctc gtcgaggtcg gcaaccatct gtttacgcgc ttcgaagcgg
     7501 tccagacccg cgtatttgtc tggaatgctt tccaatatgg cacgtgcatc ggcatcgaag
     7561 ttggcaatta aggtagtttc ttcagcctga ggttgttttg caaatttttc ccggacatac
     7621 tcggactggt tgaaattggt ttcaattttt gcattgcggg taaatatcga cccgatgtca
     7681 tggggaatac catctttgcc cttatgccgt tgccagacgg catagtcgtt gaaatcgtgc
     7741 gctggcgtga tcttcacgca gccggtgccg aattccttgt cgacgtattc atccgcgatg
     7801 atggggattt cgcgtcgggt cagcggcagt ttcaactttt tgccgaccag atgcgcatag
     7861 cgttcgtcgc tcggatcgac cgccacggcg acatcgccca gcagggtttc gggacgcgtg
     7921 gtcgccactg tgacatggcc gctgccgtcc gcgagcggat agcggatatg ccacaagaaa
     7981 ccgtcttctt cttccgacac tacttccagg tcggagaccg ccgtgcccag caccggatcc
     8041 cagttcacca gacgcttgcc gcgatagatc aggccctgct cgtacagacg cacgaacact
     8101 tccgtgaccg tcttcgacat cttcggatcc atcgtgaaat actcgcgctc ccagtcggtc
     8161 gaggcgccca tgcggcgcat ctggccggtg atggtggagc cggatttttt tttccattcc
     8221 tccacttttt cgacgaactt cttgcggccg agcgcatggc gcgatatctt ttgcgcatcg
     8281 agctggcgct ccaccacgat ctgcgtggcg atgccggcgt ggtcggtgcc tggaatccag
     8341 gcggtgttga aaccgcgcat gcgatggtag cgggtcaggc cgtccatgat ggtctggttg
     8401 aatgcatggc ccatgtgcag cgtgccggtc acgttcggcg gcggcaactg tatgctgaac
     8461 gacggttttt ccggcgcggt ggaggctgcg aaatacccgc gcttttccca ctcctcgcgc
     8521 cagaatttct caatctcggc aggctcgaaa gacttggcta attccatgat ttgatgtgtg
     8581 tttttgtgga cccgccatta taagggagga atcgctgaaa cagacttcgg ggcagcagcc
     8641 gcgcttcgat tgttgatgcc gtgattatgg cggttactac ctgaaaattt ggattcgtga
     8701 acattcacgg tgttgacgga cataaatcgg gctgttaaat tccgtgaatg ttcacggaga
     8761 caaactgaat ggatgctaca gcactgcaac gacaaaaccg gcgcatcgag cgcctgaaac
     8821 ttcagggtgt aacccgttca accgttttgg tgcatgagga atgcaagcca gcccttgaag
     8881 cattgcgtca tcacttggtt gacccggtga aggctgaggc attgaccgcc ctggtgactc
     8941 atcttcaaga caagaaggca ccgaccaatg ttgcccaggt gcgccagctc agcccattcc
     9001 ggtatccagg cggcaagaca tggcttgtgc cagaggttcg caggtggttg atgttttcga
     9061 aactcacacc ttccgttttc gttgagccgt tcgccggcgg ggcaatggcg ggccttaccg
     9121 ccgccgccga aggattagcc gaacacgttt tattgtgcga attggatgat gatgtggccg
     9181 ctgtgtgggc gactatcttt gatggtacag atgcagacgt gcgctggtta tgcgaaagca
     9241 ttaccggctt tgacgttacc ctcgataatg tccgcgaagt gcttgccagc aatccgaaga
     9301 ccaataaggg caaggcgttt cgaaccatcg tgaaaaaccg catgcaacgt ggcgggatca
     9361 tggccgccgg tgctggtctg gtgaagtccg gcgaagccgg ccgtggtctc aattcgcggt
     9421 ggtatcccga gacactggtt acacgtatca aaagactgcg cactttgcgc gaccgcgtca
     9481 tatttaaaca gggagacgcc ttcgaagcgg tgcgaaagta tgctgacgac cctaacgctt
     9541 tcttttttat tgatccaccc tataccgcgg gcggtaagaa cgctggcaag cgtttgtaca
     9601 cgcacaacga aatcgaccat gaatggttgt tcaccatcat ggcgaacgtg agcggatcgg
     9661 tcatgctgac ttatgacgat gcgcccgaag tgcgcgaaat ggccgagcgg cacggttttc
     9721 gcgtggagcc tgtaccaatg aaaaacgctc accatacaat tatccgggag ttacttattc
     9781 tgaagccttg aaaaccttgt ttgttcgtga actgtcgtca ataacttgct tgaggttccg
     9841 ggtgaaatcg gctttgctta aaggttccgt ggcgttgagc gcacgacgtg aggtgtccaa
     9901 tgtggtgaat accaccgtgc tagcagtcaa atgtagtttc atcctgctta gcggcaagct
     9961 gtccgaagct ctgccgttct tctggcgtca tcgtcgtgat gtctctaccg aaccattcgg
    10021 ctattccata gcgggcaatc ggagcacggg ccattcaata agtctcgttg tttttttgag
    10081 agaagagcat ccggcatggt atacgacaat tgccgttacc gcgccgcggc agcaaatgcg
    10141 tcgtgcgctg catcgacggt tgcggcgatg atggcgtcgt catgttgcgc cgacacgaaa
    10201 cctgcttcga atgccgaggg cgccagatag acgccggcat ccagcatggc gtggaagaag
    10261 accttgaacc ggtttttgtc accttgcatg acttcggcat aactggtcgg caccgccggt
    10321 gagaaataca ggccgaacat accgcccagc gcatcggccg aaaaagacag ctgggccgcc
    10381 ctggcggcct cgcaaagacc atcggtcagc ttgcgcgtct gtatcgacag acgggtgtag
    10441 aaaccggctt gctggacgat ccgcagcgtc gccatgcctg ctgccaccgc caccggattg
    10501 ccagacagcg t
//
LOCUS       DASRDH010000266         2895 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_281251,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000266 DASRDH010000000
VERSION     DASRDH010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2895
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281251"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..>2895
                     /gene="glnE"
                     /locus_tag="VFA14_06265"
     CDS             168..>2895
                     /gene="glnE"
                     /locus_tag="VFA14_06265"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HZS81012.1"
                     /translation="MTNCKPFPTDVTASRFLKRWANADPSRLVQLAEFSQSSMKRAVF
                     ERILQNDLDTVGEPASAAALNGSMRRLRNLVVCTLIERDLSGKADLDEVVRTMSDFAD
                     FAVQTHLHALMREMRALYGMPIGAASGVEQELIVLGMGKLGGGELNVSSDIDLIFVYP
                     EDGDTRADVPEQRSLSNHEFFIRLGRKLIAALSEITEDGFTFRVDMALRPNGNSGPLV
                     ASLDMIEEYLIRQGREWERYAWIKARALTGKAADIAALETISRPFIYRRYLDFGAIDA
                     LRSMHAQIRAEVKRQESLHPERSDNVKLGRGGIREAEFVSQVFQLIRGGRDPELRDRS
                     TRATLRTLGDKKLLAPQVAGQLLEAYIFLRNLEHRLQYLDDAQTHTLPSNENDRTIIA
                     NMMGCADAASLLAQLDTHRRIVARQFDAIFSDRQESANSNGGSDGNGVTCWLREGESI
                     ESMAAVLASLGFDDAESAARRVLATLQSPRLQSLPETSRARLMTSIDAALPVIGALSG
                     ARLPTLRRLLDFLEAIARRAAYPALLTEYPHALTRLIRMIAASDWAAQYLTRYPILLD
                     ELLDDCHLKMPPDWPAVAQDCQRQLDTAPGDTERQMDILREVHHAQLFHLLAQDLEGD
                     LSVEHLADHLSALADILVAATIQAAWKTIANRHREAPRFTVIAYGKLGGKELGYASDL
                     DLVFLYDDDDQEAPGLYARLAQRFITWMSAHTPAGTLFDIDMALRPDGASGLLVSSFA
                     TFEKYQRTSAWVWEHQALTRARYCAGDATIGNRFEALREALLRQPRDPHKLTQEVLGM
                     RMKLHQAHPNRSTQFDLKHDDGGMIDIEFIVQFLVLRYAAQYQQLTGDIGNIALLKLC
                     GALGLIDAALADEVADAYRVFRRLQHQIRLQGEECARIAIERVQQQSDSVKRL"
BASE COUNT          569 a          920 c          851 g          555 t
ORIGIN      
        1 acggaagagt aaaaactcac agtagggcgg agagctcgac tgcgggccgc attgtgttga
       61 ctacgtaccg gcgcaactac cccggcatgc cgtccggcaa tgaccatgac tgcaatcaat
      121 actggagcgt gtcgggcgcc tcgttccaaa taatctatca tggcggcatg acgaactgca
      181 aacccttccc gacagatgtg accgcttcgc gttttctgaa gcgttgggct aatgccgatc
      241 cgtcccgcct tgtccaactg gcggaatttt cccaatcatc gatgaagcgc gcggtgttcg
      301 agcgtatttt gcaaaatgat ctggataccg ttggtgagcc tgccagtgcc gccgcgttga
      361 acggctcgat gcgccgcctg cgcaacctcg tcgtctgcac gctcatcgag cgcgacctca
      421 gcggcaaggc cgatctggac gaggtagtgc gcaccatgag cgactttgcc gactttgccg
      481 tacaaacgca tctgcacgcg ctgatgcggg aaatgcgcgc gctctatggc atgcccatcg
      541 gcgccgcatc cggcgtcgaa caggagttga tcgtgctggg tatgggcaag ctgggcggcg
      601 gcgaactgaa tgtctcttcc gacatcgacc tgattttcgt ctacccggaa gatggcgaca
      661 cccgcgccga tgtacctgag cagcgttcgc tgtcgaatca cgaattcttc atccggctcg
      721 gcaggaagct gatcgccgcg ctgtcagaaa tcacggagga cggtttcacc ttccgcgtcg
      781 acatggcgct gcgcccgaat ggcaactccg gcccgttggt ggccagcctc gacatgatcg
      841 aggaatacct gatccgtcag ggccgcgaat gggagcgtta cgcttggatc aaggcgcgcg
      901 cgctgaccgg caaggccgcc gacatcgctg cgctggaaac catcagtcgt ccctttatct
      961 atcgtcgcta tctggatttc ggcgcgatcg acgcgctgcg ctccatgcat gcgcagattc
     1021 gcgccgaagt caaacgccag gaatccctgc atccggaacg tagcgacaac gtcaagctgg
     1081 ggcgcggcgg catccgcgag gcagagttcg tcagccaggt gttccagctc attcgcggtg
     1141 gccgcgatcc ggagctgcgc gaccgctcca ctcgcgctac tctgcgcacg ctgggggaca
     1201 aaaaactgct ggcaccgcag gtagccgggc aactgctgga agcctacatc ttcctgcgca
     1261 atctcgaaca tcgcctgcaa tatctcgacg acgcccagac gcatacgctg ccgtccaatg
     1321 aaaacgaccg gactatcatt gccaatatga tgggttgcgc cgatgcggca tccctgctgg
     1381 cgcagctcga cacgcatcgc cgcatcgtcg cgcgacaatt cgatgcgatc ttcagcgaca
     1441 ggcaggagag tgctaacagt aacggcggca gtgacggcaa tggcgtcaca tgctggctgc
     1501 gcgagggaga aagcatcgaa tcgatggccg cggttctggc atcactcggc ttcgacgatg
     1561 ctgaaagcgc tgcccggcgt gtactcgcga cgttgcaatc gccgcgtctg caatcgctgc
     1621 cggaaaccag ccgcgcgcgc ctcatgacat cgatcgatgc ggccctgcct gtcatcggcg
     1681 cgctgtccgg cgcgcgtctg ccgaccttgc gccgtctgct ggatttcctg gaagcgattg
     1741 cccgccgcgc cgcttatccg gcgctgctga ccgagtaccc gcatgcattg acgcgcctga
     1801 tccgcatgat tgccgccagc gactgggcgg cacaatacct gactcgctat cccatcctgc
     1861 tcgacgagct gctcgacgac tgccatctga agatgccgcc ggactggcca gccgttgcgc
     1921 aagactgtca gcgccagctc gacaccgcgc cgggcgacac cgagcggcag atggacatcc
     1981 tgcgcgaagt tcaccatgct caactgttcc atctgctggc gcaggatcta gaaggcgatc
     2041 tgagcgttga acacctggcc gatcacctct cggcactggc cgacatcctg gttgctgcca
     2101 ccatccaggc agcctggaaa accatcgcca atcgccatcg tgaagcaccg cgattcaccg
     2161 tgatcgccta cggcaaactc ggcggcaagg aactgggcta cgcttctgat ctcgacctcg
     2221 tctttcttta cgacgacgac gaccaggagg cacctggtct ctacgcccgg ctggcgcagc
     2281 gcttcatcac ctggatgagt gcgcatacgc cggctggtac gctgttcgac atcgatatgg
     2341 ccttacgtcc tgatggcgcc agcggcctgc tggtatcgtc gttcgcgacg ttcgaaaaat
     2401 atcagcggac gtctgcctgg gtgtgggaac atcaggcgct cacgcgcgcg cgctattgcg
     2461 ccggtgatgc caccatcggt aaccgtttcg aggccttgcg cgaagccttg ctgcgccagc
     2521 cgcgcgatcc gcacaaactg acgcaggaag tactcggcat gcgcatgaag ctgcatcagg
     2581 cacatcccaa tcgcagtacg cagttcgacc tcaaacacga cgatggtggc atgatcgaca
     2641 tcgaattcat cgtgcagttt ctggtgttgc gctacgctgc acaatatcag cagttgactg
     2701 gcgacatcgg caacatcgcc ctgctgaaac tatgcggcgc gcttggtctg atcgacgctg
     2761 cgctggccga tgaggtcgcc gacgcttatc gcgtgttccg caggttacag catcagatcc
     2821 gcctgcaagg cgaggagtgc gcccgcatcg ccatcgaacg cgtacagcag caaagcgaca
     2881 gtgtcaagcg gctgt
//
LOCUS       DASRDH010000267         2388 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1433085,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000267 DASRDH010000000
VERSION     DASRDH010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2388
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1433085"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..861)
                     /locus_tag="VFA14_06270"
     CDS             complement(<1..861)
                     /locus_tag="VFA14_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016836001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HZS81013.1"
                     /translation="MIGNTASIQLKTRQRWIADAVELFSSMRFAISLLALIAVASIIG
                     TVLRQNEPMPSYVNQFGPFWFEVFDRLRLYDVYLSWWFLLIIGCLVLSTSLCLARNTP
                     KILRDISSWRENVREQSLRNFHHKTEWTVARSPTDLIRQLVRCIRANGYQVKVVEKHD
                     AALIAARKGAANKWGYLFAHGAIVIICLGGLLDSGLPIRFQQWFHGKTPFAGSGLIAQ
                     VPAQSRLGPGNPAFRGNTLIAEGGSSGTAIIAQRNGVMIQDLPFTIRLERFIIDYYST
                     GMPKLFASEVI"
     gene            complement(1159..1443)
                     /locus_tag="VFA14_06275"
     CDS             complement(1159..1443)
                     /locus_tag="VFA14_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS81014.1"
                     /translation="MARKDNDGDHLGFETECKEEAALQDFPQFLLKVARFKGTTRDEF
                     LDNRQFHGNAFALMHRAEQFLIESLPIAGRIGRAWMASSFANGPDNRENA"
     gene            1732..2010
                     /gene="yajC"
                     /locus_tag="VFA14_06280"
     CDS             1732..2010
                     /gene="yajC"
                     /locus_tag="VFA14_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007877772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HZS81015.1"
                     /translation="MGNLASFLPIILMFVVLYFLMIRPQMKRQKEQKAMLDALAKGDE
                     VVTSGGLLGRLLKVSDAYVTVEVSEGNEIVVQKAAITTLLPKGTLKGL"
     gene            2129..>2388
                     /locus_tag="VFA14_06285"
     CDS             2129..>2388
                     /locus_tag="VFA14_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008116429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HZS81016.1"
                     /translation="MNRYPFWKYVLIAIALLFGTLYTLPNFFGESPAVQISSAKSTIK
                     VDSLVAERVRQILQQGKLAPESIVFDDSGAPSVRARFVNTDI"
BASE COUNT          504 a          647 c          685 g          552 t
ORIGIN      
        1 gatcacctca ctggcgaaca gtttgggcat gccggtcgag taataatcga tgatgaagcg
       61 ctcgagtcga atcgtgaatg gcagatcctg aatcatcacg ccattccgct gtgcaatgat
      121 ggcggtgccg ctgctgcctc cttccgcgat cagtgtattg ccgcggaacg ccggattgcc
      181 cggacccaga cgggattgcg ccggaacctg cgcaatcagg ccgctgccgg caaacggcgt
      241 cttgccatga aaccattgct gaaagcggat cggcagaccg gaatcgagca gaccgcccag
      301 gcagatgatg acgatggcac catgcgcgaa gagatagccc catttattgg cagcgccttt
      361 cctggcagca atcagagcgg catcatgttt ttccacgacc ttgacctgat agccgttggc
      421 gcggatgcat cgaaccagtt gccgtatcaa atcggtggga gagcgggcaa ccgtccattc
      481 ggttttatgg tgaaaattgc gcagcgactg ttcacgcacg ttctcgcgcc agctactgat
      541 atccctgagt atttttggcg tgttacgcgc aaggcacagc gaggtcgata gcaccagaca
      601 ccctatgatc agcagaaacc accagcttaa atagacatcg tataacctca gcctgtcgaa
      661 tacctcgaac cagaacggac cgaactgatt cacataactg ggcattggct cgttctgcct
      721 gagtacggtg ccgatgatgg aagcgaccgc tatcagcgcc agcagactga tcgcaaagcg
      781 catggaagaa aatagttcga ccgcatcagc aatccagcgt tgccgcgtct tcagttgtat
      841 gctggcggta ttacctatca tgagtacgac ctttatcgat gctgtctgta cggtgtttca
      901 gtgactgtca tgctactgat gtggcgaaaa tttcggacca gccgctcggc gtagaaggcc
      961 atcatcacgg ctcttcggta ccggtatcca gcacctgcca atgcccgcct ttgttcgggc
     1021 cgatgcgttg caggcggccg gattcacgca gcttgtgatg gcacggtgga tggttagttc
     1081 cgacttgccc aggcgttgcg ccagttcggg tgccgtcacg tcaggtcagg cgctgcggct
     1141 agcaggcgca acacttcatc aggcgttttc cctgttgtca gggccatttg cgaaggagct
     1201 ggccatccac gcgcggccga tgcggccggc gatcggcagc gactcgatca ggaattgctc
     1261 cgctcggtgc atcagcgcga aagcattacc gtgaaactga cgattgtcga gaaattcatc
     1321 gcgcgtggtg cccttgaagc gggccacttt caacagaaac tgcgggaaat cctgcagcgc
     1381 cgcttcctcc ttgcattctg tttcgaagcc gagatgatca ccgtcgttgt cttttcttgc
     1441 cattgcgtat gctcctgtgc gcgtgccgga tcgttcagca cgaccgaaaa tccggctact
     1501 ggcccggatc gtcggggcag ctgggaagga tggaagttta tcaggccggc atacaggtga
     1561 ggggggagga ggtcggtcaa gcctgttttc cctgtttttc cttgcccgga atggggcgag
     1621 tacccggtgc agtgctagaa tgcggggtca tttttaaaca actgtttgga gcgatacgtg
     1681 tttatttccg atgcaactgc acaaaccacg gctgcgggtt ccgtcggcct gatgggtaat
     1741 ctggccagtt ttctgcctat catcctgatg ttcgttgtgc tgtactttct gatgattcgc
     1801 cctcagatga agcgtcaaaa agaacaaaag gccatgctgg acgcccttgc caagggtgat
     1861 gaagtcgtca cttccggcgg tctgttgggc aggctcctca aagtcagtga tgcttatgtg
     1921 actgtcgaag tctccgaagg caatgaaatt gtcgtgcaga aggcagctat taccacactg
     1981 ctgccgaagg gtacgctcaa gggactgtaa gaacgtgaac acgttctgac cggtccgggg
     2041 ccggctgacg tgggggcgca ttccgcaggg cgtggatgtg cccatgcttc tatccgatat
     2101 ggatattcat catcgacgct gaatcattat gaatcgttat cctttctgga agtacgtcct
     2161 gattgccatt gccctgctgt tcggcacact gtatacactg cccaattttt tcggcgaatc
     2221 gccggctgtg cagatcagca gcgccaaatc caccatcaag gtagatagcc tggtggctga
     2281 gcgtgtgcgg cagatcctgc aacagggcaa gctcgcgccg gagagcatcg ttttcgatga
     2341 cagtggcgcg ccttccgtgc gtgcccgatt cgttaatacc gatatcca
//
LOCUS       DASRDH010000268         2536 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_347252,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000268 DASRDH010000000
VERSION     DASRDH010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347252"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="VFA14_06290"
     CDS             <1..253
                     /locus_tag="VFA14_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007879121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HZS81017.1"
                     /translation="AMTFPRALQIRRLAAGVPLDAIVLETDAPDMAPAWLHPARNTPD
                     QIPRIGEVLAALRGISADRVAQATCANACAALPRLASLC"
     gene            257..>2536
                     /locus_tag="VFA14_06295"
     CDS             257..>2536
                     /locus_tag="VFA14_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007879120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HZS81018.1"
                     /translation="MRSATLGFAAGIALLQMQARLLPGAWYGVLLAVTAILLLTGWRL
                     RRTGVHVVWRIVAFALAGMCFGFAWGGLYAWHYLDEELPLAWEGRDVTVVGTVDSLPY
                     RFERGVRFNFQVEKIAPQGMETPQIPARLSLAWYAAGSFSKGNAVSAAAVPDLRPGER
                     WQLTVRLRRPHGSANPHGFDYEVWLLEQGIRATGSVRPDAQPATRNHRLQSFVWTPGH
                     VIEYCRGWLRERIFAALPGQAYAGVIVALVIGEQRAIDQSEWTVFNRTGIAHLVAISG
                     LHITMIAGLFAACAGFLWRRSFFTRAQLPLLFPAQKVAVLAGLLSALFYTLLAGFGIP
                     AQRTLYMLAVVGAAFWMGRLTPISCVMCLALGVVLLADPWALLWPGFWLSFSAVAIIL
                     YASSGRVGNGTAMEAQAFIVMQRCRRAARLWRVLREGAWTQYVVTLGLLPLTLLLFGQ
                     YSLVSPLANALAIPLITLLVAPLSLLGSIVPAPLSAWILGLAHALLEWLVGLLSCLSS
                     QPFAVWETSLPSPWMFLLALAGTLLLLAPRGWPARWLGWFGWLPLALNGATWPAEGQM
                     RVTALDVGQGMALLVETAQRRLLYDAGPAWSTESDAGNRVILPYLRMRGIHHLDVMVI
                     SHSDIDHAGGALSVLREIAVDRVYSSLPFTHPVAQAAPDHRRCLAGQYWNWDGAAFSM
                     LYPVQSIYDSGKWKPNARSCVLKVALGKSAILLPGDIEAVQESELVNSDTAADLRATV
                     LLAPHHGSGTSSTEAFLRAV"
BASE COUNT          338 a          690 c          881 g          627 t
ORIGIN      
        1 tgccatgact tttccgcgtg ccttgcagat tcgtcgtctg gctgctggcg tgccgctgga
       61 cgccattgtg ctggaaaccg atgcgccgga tatggctccc gcctggctgc atccggcacg
      121 gaatacgccg gaccagattc cgcgcatcgg cgaggtgctg gctgcactgc gcggcatcag
      181 cgccgaccgg gtggcgcagg cgacgtgcgc caatgcgtgt gcggcgttgc cgcgtctggc
      241 ctcactctgt tgattcgtgc gcagcgcgac tctcggtttt gccgcaggaa tagcgttgtt
      301 gcagatgcag gctcggctgc tgcctggcgc atggtacggg gtgctgctgg ccgtgacagc
      361 gatcctgctg ttgactggct ggcgtttgcg ccggactggc gtgcatgtcg tgtggcgtat
      421 agtggccttt gcgctcgccg ggatgtgttt cgggtttgcc tggggtggtc tgtatgcctg
      481 gcactatctg gatgaagagt tgccgcttgc ttgggagggt cgcgacgtga cggtcgtggg
      541 cactgtggat agcttgccgt accgcttcga gcgtggtgtg cgtttcaatt tccaggtgga
      601 aaaaattgcg ccgcagggca tggaaacgcc gcagattcct gcccgattgt cgctcgcctg
      661 gtatgcggcc gggtctttca gcaagggcaa tgcggtgtct gcggctgccg ttcctgatct
      721 gcggcccggc gagcgctggc agttgaccgt gcgtctcagg cgtccgcatg gcagcgccaa
      781 tccgcatggc ttcgattatg aagtatggtt gctggagcag ggcatacgcg cgaccggcag
      841 cgtgcgtcct gacgcgcaac cggcgaccag gaatcatcgt ttgcagtcct tcgtctggac
      901 gccgggccat gtcatcgagt attgccgggg ctggttgcgt gagcgtattt tcgcggcctt
      961 gcctggacaa gcctatgctg gtgtcatcgt tgcgctggtg atcggtgagc agcgtgccat
     1021 cgaccagtcc gagtggactg ttttcaatcg aaccgggatt gctcatctgg tggctatttc
     1081 aggcttgcat atcaccatga ttgctggtct gttcgcggcc tgcgccggtt ttttgtggcg
     1141 ccgttctttt tttacgcggg cgcaattgcc cctgcttttt ccggcgcaaa aagtcgccgt
     1201 gctggctggt cttctgtctg ctttgttcta tactttgctg gctggctttg gtattccagc
     1261 acagcgcacg ctgtatatgc tggcggtggt tggcgcggcg ttctggatgg gacggttgac
     1321 gccgatttcc tgtgtgatgt gtcttgcgct cggtgtggtg ctgctggctg atccctgggc
     1381 gctgttatgg ccgggtttct ggttgtcgtt cagcgctgtg gcgatcattc tgtatgcatc
     1441 gtccgggcgg gtcggcaatg gcaccgcgat ggaagcgcaa gcgtttatcg tcatgcaacg
     1501 gtgccgcaga gcggcgcggc tgtggcgggt gttgcgcgag ggcgcctgga cgcagtatgt
     1561 cgtgacgctc ggtttattgc cgctgacttt gctactgttc ggccagtact ccctggtgag
     1621 tccgctggcc aatgcgctgg cgattccgct gatcactttg ctggtggcgc ctttgtcgct
     1681 gctcggcagc atcgtgccgg cgccgctgtc ggcatggata ttgggattgg cgcatgcgtt
     1741 gctggagtgg ctggtcggtt tgctgagctg cttgagcagt caacctttcg cagtctggga
     1801 aacgtcgctg ccgtcaccat ggatgttttt gctggcgctg gcggggactt tgctgttgtt
     1861 ggcgccgcgt gggtggccgg cgcgctggct gggatggttc ggctggctgc cgctggcgct
     1921 caacggtgcg acctggccgg cagaagggca gatgcgggta actgccctcg atgtcggtca
     1981 aggcatggcg ctgctggtgg aaactgcgca gcgccgtctg ctttacgatg ccgggccggc
     2041 ctggtcgacg gagtcggatg ccggcaaccg ggtcattctg ccgtatctgc ggatgcgcgg
     2101 cattcatcat ctggatgtga tggtgatttc gcacagcgac atcgaccatg ccggcggcgc
     2161 gttgtcggta ctacgtgaga ttgctgtcga tcgcgtttac tcttctctgc cgttcacgca
     2221 tcctgtcgcg caggccgcac cggatcatcg gcgctgcctt gcgggacagt actggaactg
     2281 ggatggcgcg gcgttcagca tgctgtatcc ggtccagtcc atttacgaca gcgggaagtg
     2341 gaagcccaat gcgcgcagct gtgtgctgaa agtcgccctg ggcaaaagtg ccatcctgct
     2401 gccgggcgat atcgaagccg tccaggagag tgaactcgtg aatagtgaca cggccgctga
     2461 tttgcgggcg acggtgctgc tggcgccgca tcatggcagt ggtacgtcgt ccacggaagc
     2521 gtttctgcgt gcggtc
//
LOCUS       DASRDH010000269         3011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1434167,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000269 DASRDH010000000
VERSION     DASRDH010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 3011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3011
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1434167"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="VFA14_06300"
     CDS             complement(<1..407)
                     /locus_tag="VFA14_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HZS81019.1"
                     /translation="MVIDTGKTAPRGEASSASLAAIDEFCDTLWLEDGLARNSLEAYR
                     RDMTLFARWLQSQRHKDLLATSQDDINAYFAARHADTRASSANRRLAVLKRFFLLALR
                     QNRIAEDPCLYMKPARQPPRFPRVLSEAQVDAL"
     gene            complement(397..894)
                     /locus_tag="VFA14_06305"
     CDS             complement(397..894)
                     /locus_tag="VFA14_06305"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017454832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HZS81020.1"
                     /translation="MMRAQAGDIFSAIVHIPFGGVGIRTSAGVIQEMAYLPRHFREKD
                     AIDALADKAARQILRYIDGPDYRFSLPLAEVGSVFQRKVWAAISSIPRGETCTYGQIA
                     RYIQSAPRAVGQACGANRFPLIVPCHRVTASDGLGGFSHHDDADGFPLSVKRWLLMHE
                     GVDGY"
     gene            1401..2330
                     /gene="pqqB"
                     /locus_tag="VFA14_06310"
     CDS             1401..2330
                     /gene="pqqB"
                     /locus_tag="VFA14_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012355287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqB"
                     /protein_id="HZS81021.1"
                     /translation="MLHVIVLGAAAGGGLPQWNCACKMCQAAREGGLVVPQTQSSIAI
                     SADGTHWVLVNASPDVRQQFSATPALHPRQTPRSSPLHAVLLTNADVDHIAGLLSLRE
                     SQAFAVYATSRVQRVLEANAVFNVLSREHVVRRTYTMDQLQPVLDAAGKDTGVRVEAF
                     PIPGKIALWLEDPNAIQLGSVPEDTVGLALSAVGSSSRLFYLPGCAAVPDAIKARLQP
                     EDSLLFDGTTFTEDEMITSGMGHKTASRMGHLPMSGDTGAMAQWKDVPLKRRLFIHIN
                     NTNPVLLADSPERSIIRAAGWQLAYDGMELHLE"
     gene            2323..>3011
                     /gene="pqqC"
                     /locus_tag="VFA14_06315"
     CDS             2323..>3011
                     /gene="pqqC"
                     /locus_tag="VFA14_06315"
                     /EC_number="1.3.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012355288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline-quinone synthase PqqC"
                     /protein_id="HZS81022.1"
                     /translation="MNKPLTAVLSPAGLEAALRAIGDARYHSRHPFHHLLHGGKLNLG
                     QVQAWALNRYCYQAAIPRKDAALISRLDDRELRREWIHRILDHDGYGNEKGGIERWLA
                     LTDGLGLKRDYVVSRRGALPATIFAVEAYVDFVARHPPVIAVASCLTELFAPAIHQER
                     IVGMLENYDFIDEPVLAYFRRRLTQAPDDAAFALDYVKRHALTLTEQQSVLDALTFKC
                     HVLWAQLDALYL"
BASE COUNT          641 a          884 c          832 g          654 t
ORIGIN      
        1 aacaaggcat cgacctgcgc ctctgacaaa accctgggaa aacgcggcgg ctgcctggcc
       61 ggcttcatgt acaagcaggg atcttcggcg atgcggtttt gccgcagggc cagcaggaag
      121 aaacgcttca gtaccgccag acgtcgattg gctgaactgg ccctggtatc ggcatggcga
      181 gcggcaaaat aagcgttgat gtcatcctga ctggtcgcga gcaaatcctt gtggcgctgg
      241 ctttgcaacc agcgcgcaaa cagtgtcatg tcgcgccggt aggcttccag tgaattcctg
      301 gccagaccgt cttccagcca tagcgtatcg cagaattcat cgatggcagc caggctggcg
      361 ctgctggctt cgcccctggg agcggttttt ccagtgtcaa taaccatcga caccttcgtg
      421 catcagcagc caacgtttca cgctcagcgg aaaaccatct gcatcgtcgt gatgcgagaa
      481 tccgccaagc ccatccgatg ccgtcacacg atgacagggc acgatcaaag gaaaccggtt
      541 ggcgccgcat gcctgtccaa ccgcgcgcgg cgcggattga atatatctgg caatctggcc
      601 gtaagtgcat gtctcaccgc gcggaatgga tgaaatcgcc gcccagacct tgcgctgaaa
      661 tacggagccg acctcggcca gaggcaagct gaaacgatag tcaggcccgt caatatagcg
      721 cagaatctgt ctggctgcct tgtcggccag tgcatcgatg gcgtcttttt cgcggaaatg
      781 ccgcggcaga tacgccatct cttgaatcac gccagccgac gtccggatac cgacgccgcc
      841 gaagggaatg tgtacgatgg cagagaaaat gtcgccagct tgtgctctca tcatgcgaaa
      901 acggaaaaga aatacgctat ttaagcatat cgccgccgtc ccttgccata cctgctgcta
      961 gacaatccat tgtcttgcat accggcatga cctggcgatt cttcttgcct tgatttggca
     1021 ggaagaatga attacgataa ttcgtcagaa gtttttctgt ttgaaaggaa tagtggctga
     1081 cctgggcagc cgtcgttccc gctcaatcaa ccgcctgtcg cgataggata ctgagccctg
     1141 atggcaacac gatccatgtg gcgaaagatt cgggtggatt gagcgctact gacacaggca
     1201 atgcggggtt caggctgaac cattctgacg tgatccgaat gttcggcaga aatcaccgct
     1261 tgctgaaatg gcccagcaat tttgcatttt atctggagat cacactatgc ttacctggac
     1321 tactccgcaa gtcatcgagg tctgcctcgg ttgtgaaatc aactgctata tgagcgccga
     1381 gggctgagtt cggctctctt atgttgcatg tcatcgttct cggcgcggcg gccggcggcg
     1441 gtttgccgca atggaactgc gcttgcaaaa tgtgccaggc tgcccgcgag ggtggcctgg
     1501 ttgtaccgca gacgcaatct tcgattgcca tatcagctga tggaacgcat tgggtgctgg
     1561 tcaatgcctc tcctgacgtc cgccagcaat tttccgccac tccagcgctg catcccaggc
     1621 agacaccacg ttccagccct ttgcatgccg tgctactgac caacgccgat gtcgatcata
     1681 ttgccggcct gttatcattg cgcgagagtc aggcatttgc cgtctatgca accagccgtg
     1741 tgcagcgcgt gcttgaagcc aatgcagtat tcaatgtgct ctctcgggaa catgtcgtcc
     1801 gtcggaccta tactatggat cagcttcaac ccgtactcga tgccgcaggc aaagacaccg
     1861 gcgtgcgcgt cgaagccttt cccatccccg gcaagatcgc gctgtggctc gaagacccga
     1921 atgctatcca gcttggcagc gtgccggaag atactgttgg cctggcgctg agcgctgtcg
     1981 gctccagcag ccggctattc tacctgccgg gctgcgccgc cgttcccgat gccatcaagg
     2041 cgcgcttgca gcctgaagac agcctgttgt tcgacggtac tacgttcacc gaagacgaaa
     2101 tgattaccag cggcatgggt cataaaacgg cgtcccgcat gggccactta ccgatgagcg
     2161 gcgacaccgg cgcaatggcg cagtggaaag acgtgccact caagcgcagg ctattcattc
     2221 atatcaacaa taccaatccc gttttgctcg cggacagccc ggaacgctcc attatccgtg
     2281 cggcaggctg gcagctggca tacgacggca tggaactcca tcttgaataa accattgact
     2341 gccgtgctgt ccccggctgg actggaagca gcgctacgcg ccattggtga tgcacgctat
     2401 cacagccggc atcctttcca tcatctgctg catggcggca agctgaacct cgggcaagtt
     2461 caggcgtggg cgctcaatcg ctactgctat caggctgcca ttccacgcaa ggatgctgcg
     2521 ttgatcagcc gtttggatga ccgtgaacta cgccgagaat ggatacatcg cattctcgat
     2581 cacgatggct acggcaacga aaaaggcggc atcgaacgct ggctggcact cactgacggg
     2641 ctgggtctga aacgcgacta tgtcgtttca cgtcgtggcg cgctaccggc cacaatattt
     2701 gccgtggagg cttatgttga cttcgtcgcg cgccatccgc ctgtcattgc cgtggcttcc
     2761 tgcctgacgg aactgttcgc tccggcgatt caccaggagc gtatcgtcgg catgcttgaa
     2821 aactacgatt ttatcgatga gccggtgttg gcctatttcc ggcgtcggct aacccaggca
     2881 cccgacgacg ccgcatttgc cctcgactat gtcaagcgcc atgcactgac cctgaccgag
     2941 caacagagcg tgctggacgc gctgacgttc aaatgtcacg tgctttgggc acaactcgat
     3001 gcactctacc t
//
LOCUS       DASRDH010000270         3471 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_760275,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000270 DASRDH010000000
VERSION     DASRDH010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 3471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3471
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_760275"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="VFA14_06320"
     CDS             <1..252
                     /locus_tag="VFA14_06320"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013106725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HZS81023.1"
                     /translation="VQSDLLIDLESVVLCPVTSDLRNAAFRVTVEPNPANGLHALSQV
                     MVDKLSTLPRSKISGPFGHLDDERMKAIDRALLLVVGVI"
     gene            487..>3471
                     /locus_tag="VFA14_06325"
     CDS             487..>3471
                     /locus_tag="VFA14_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase central domain-containing
                     protein"
                     /protein_id="HZS81024.1"
                     /translation="MHAQGLYDPVNERDACGVGFIAHIKGKKSHAIVEQGLLILKNLD
                     HRGAVGADPLMGDGAGILIQIPDRYYREEMAQQGVELPPPGEYGVGMIFLPKEHASRI
                     ACEQEIERTVLAEGQVVLGWRDVPVDRDMPMSLTVREKEPVIRQIFIGRGPDILVTDA
                     LERKLYVIRKSSGHAIQALSLIHGKEFFVPSMSARTVVYKGLLLADQVGVYYRDLQDQ
                     RCVSALALVHQRFSTNTFPEWPLTHPYRMIAHNGEINTVKGNFNWMRAREGVMQSAVL
                     GDDLKKLFPLIYEGQSDTACFDNALELLCMAGYPLAQAMMMMVPEAWENHTLMDDNRR
                     AFYEYHAAMMEPWDGPAALAFTDGRHIGGTLDRNGLRPARYIVTDDDLVVMASESGVL
                     PIPESRIIQKWRLQPGKMFLIDLDAGRIIDDKELKDTYANAKPYKQWIASVRVKLDEL
                     KAEPPVSGSTMTLLDRQQIFGYTQEDIKLLMAPMALTGEEAIGSMGNDAPLAVMSSKN
                     KTLYSYFRQLFAQVTNPPIDPIREAMVMSLVSFIGPRPNLLDTNNINPPMRLEVSQPV
                     LNYADIVRLRNISAHTGGKFKSHELDICYPVAWGKEGIEACLASLCAKAVDAVRSGHN
                     ILIVSDRKVDAERVAIPALLATSAVHLHLVSKGLRTSTGLVVETGSARETHHFALLAG
                     YGAEAIHPYLAMESLTDMVRDLPGDLSGDKAISNFQKAIGKGLLKVMSKMGISTYMSY
                     CGAQIFEAIGLNRALVDKYFKGTSSSVEGIGVFEVAEEALCLHRAAFSNDPVLANALD
                     AGGEYVFRIRGEEHMWTPDAIAGLQHATRANSFNSYKEYAQIINDQSRRHMTLRGLFE
                     FRIDPSRAISIDTVEPAQEIVKRFATGAMSLGSISTEAHATLAIAMNRIGGKSNTGEG
                     GEDVNRYRNELKGIPIRQGATLASEIGKEVVVVDIPLQAGDSLRSRIKQVASGRFGVS
                     AEYLISADQIQIKMAQGAKPGEG"
BASE COUNT          749 a          971 c         1014 g          737 t
ORIGIN      
        1 gttcaatcag acttgctaat cgacttggag agtgtcgtgt tgtgtccggt gacaagcgat
       61 ctgcgtaatg ccgcgtttcg cgtcactgtg gaaccaaatc cggccaatgg gctacacgca
      121 ctatcgcaag tcatggtaga caagctctcg acgttgccgc gctccaaaat cagcggaccg
      181 tttggccacc tcgatgatga gcggatgaaa gcgatagata gggcgctctt gctggtcgtc
      241 ggtgttattt gacaccagcc gccatctggc ggctggtgac acgactgctt gcctctggct
      301 ggagaaatct ggaattccgg cttactcagt atcacggcgg agttttcttt tactctgacc
      361 ccaattaata aaaaatagaa ccataagagc tttcaattct actcagaccc tatttatttt
      421 ctgttgaaga tttgtccatg ctgcactatt cttcgttcct tactcttaaa gtgatggaac
      481 attgtcatgc atgcgcaagg tctatacgat ccggtgaatg aacgcgacgc ttgcggcgtt
      541 ggttttatcg ctcatatcaa agggaagaaa agccatgcca tcgttgagca aggcttgctc
      601 atcctgaaga atctcgacca ccggggcgcg gtcggcgccg atccgctgat gggtgacggc
      661 gccggcatcc tgatccagat cccggatcga tactatcgtg aggaaatggc gcagcagggc
      721 gttgaactgc cgccgcccgg tgaatacggc gttggcatga tcttcctgcc gaaggaacat
      781 gcctcgcgca ttgcctgtga acaggaaatc gaacgcaccg tgctggccga aggacaggtc
      841 gtattgggct ggcgtgacgt gccggtcgac agggacatgc cgatgtcact gaccgtgcgt
      901 gaaaaagaac ccgtcatccg ccagatcttc atcggtcgcg gtccggacat cttggtgacc
      961 gacgcactgg aacgtaaact gtacgtcatc cgtaaatcgt ccggccatgc catccaggcg
     1021 ctgagcctga tccatggcaa ggaatttttt gtgccgtcga tgtcggcgcg caccgtcgtg
     1081 tacaagggcc tactgctggc ggatcaggtc ggcgtgtatt accgggatct acaggatcag
     1141 cgctgcgtat ccgcgctggc gctggtgcat caacgcttct ccaccaatac tttccccgag
     1201 tggccgctga cgcatcccta ccgcatgatc gcccataacg gcgaaatcaa cactgtcaag
     1261 ggcaatttca actggatgcg cgcccgcgaa ggcgtgatgc agtcggctgt actgggcgac
     1321 gatctgaaga agctgtttcc actgatttat gaaggacagt ccgataccgc gtgtttcgat
     1381 aacgcgctgg aactgttgtg catggcaggt tatccgctgg cgcaagcgat gatgatgatg
     1441 gtgccggaag cctgggaaaa ccacacgctg atggacgaca accgtcgtgc cttctatgaa
     1501 taccacgccg cgatgatgga gccatgggat ggcccggccg cgctggcttt cactgatggc
     1561 cgtcatatcg gcgggacgct ggaccgcaac ggcctgcgtc ccgcacgtta tatcgtgact
     1621 gatgacgatc tggtggtgat ggcgtccgag tccggcgtgt tgccgattcc ggaatccagg
     1681 atcattcaga aatggcgtct gcaaccaggc aagatgttcc tgatcgatct cgacgctggc
     1741 cgcatcatcg acgacaagga actgaaggat acctacgcca acgccaagcc gtacaagcaa
     1801 tggattgcgt cggtacgtgt caagctggac gaactgaaag ccgagccgcc ggtatccggt
     1861 tcgaccatga cgctgctgga tcgccagcag atattcggtt atacccagga agacatcaaa
     1921 ctcctgatgg cgccgatggc cctgacaggc gaggaagcga tcggctcgat gggcaacgac
     1981 gcgccgctgg ccgtcatgtc cagcaagaac aagacgctct acagctactt caggcaacta
     2041 ttcgcgcaag tgaccaatcc gccgatcgac ccgatccgcg aagccatggt catgtcgctg
     2101 gtgtccttca tcggcccgcg cccgaatctg ctcgatacca ataacatcaa cccgccgatg
     2161 cggctggaag tatcgcagcc ggtgctgaac tacgccgaca tcgtcaggtt gcgtaacatc
     2221 agcgcacata ccggtggcaa gttcaagtcg catgagctgg acatctgtta tccggtcgcc
     2281 tggggcaagg agggcatcga agcctgcctg gcctcgttat gcgccaaggc cgtcgatgcg
     2341 gttcggtcgg gccataacat cctgatcgtc tcggatcgca aggttgatgc cgaacgggtc
     2401 gcgattccgg cgttgctggc gacttcggca gtccacctgc atctggtcag caagggtctg
     2461 cgcacttcga ccggtctggt cgtggaaacc ggttccgcgc gtgaaacgca tcatttcgcc
     2521 ttgctggccg gttacggcgc tgaagcgatt cacccttacc tcgcgatgga gtcgctgacc
     2581 gacatggtca gggatctgcc gggtgatctg tcaggtgaca aggcgatcag taacttccag
     2641 aaagcgatcg gcaagggctt gctgaaggtc atgtcgaaga tggggatttc tacctatatg
     2701 tcttactgcg gtgcgcagat tttcgaagcc atcggtctga accgggcgct ggtcgataaa
     2761 tacttcaagg ggacgtcttc cagcgtcgag ggtattggcg tgttcgaagt cgccgaagaa
     2821 gcgctgtgtc tgcaccgtgc cgcattcagc aacgacccgg tgctggccaa tgcgctggac
     2881 gccggtggtg aatacgtctt ccggatccgc ggcgaagagc atatgtggac gccggatgcg
     2941 atcgccggat tgcaacatgc gacccgcgcg aacagcttca attcgtacaa ggaatatgcg
     3001 cagatcatca atgatcaatc caggcgtcac atgaccttgc gcggcctgtt cgaattccgg
     3061 atcgatccgt ccagagccat ctctatcgat accgtcgaac cggcgcagga aatcgtcaag
     3121 cgctttgcca ccggcgccat gtcgctcggt tcgatctcga ccgaagcgca cgccacgctg
     3181 gccattgcga tgaaccgcat cggcggtaag tccaacaccg gcgaaggcgg cgaagatgtc
     3241 aatcgctacc gcaacgaatt gaaaggtatc ccgatcaggc agggcgcaac cctggcgtcc
     3301 gaaattggca aggaggtggt ggttgtcgac attccgctgc aagccggcga ctcgctgcgt
     3361 tcacgcatca agcaggtagc gtccggtcgt ttcggtgtgt cggctgaata cctgatttcc
     3421 gccgatcaga ttcaaatcaa gatggcgcag ggcgccaagc cgggcgaagg c
//
LOCUS       DASRDH010000271         2672 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_768503,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000271 DASRDH010000000
VERSION     DASRDH010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_768503"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1378)
                     /gene="edd"
                     /locus_tag="VFA14_06330"
     CDS             complement(<1..1378)
                     /gene="edd"
                     /locus_tag="VFA14_06330"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HZS81025.1"
                     /translation="MSLHATLASVTRRIAERSRPYRSAYLARLDAARHGGVQRSALSC
                     TNLAHGFAAFTANDKLKLKQDRAPSVAIVSSYNDMLSAHQPFDGFPRIIRDAIRADGG
                     VAQFAGGVPAMCDGITQGQTGMELSLFSRDTIAMGTAIALSHNMFDSALYLGVCDKIV
                     PGLLIGALHFGHIPAVFVPAGPMTSGMSNSEKLKIRQRYAEGKIGRAELLEAEAQSYH
                     DAGTCTFYGTANSNQMLMEALGLHLPGSAFVTPNTPLRDALTAAAARQALKITAQGTQ
                     YTPVGRLVDEKSIVNAIVALLATGGSTNHTLHLVAIAKAAGLVIDWDDFSRLSSVAPL
                     ITRIYPNGSADVNHFHAAGGAGFVLRELLDAGLMHDDVMTILGHGLRAHCAEPLLEDG
                     RAVWKEAPQNSGDETVLRPATNPFSADGGLKLLQGNLGRSVIKVSAVQQAHRAVRAPA
                     IVFHSQEAF"
     gene            1560..>2672
                     /gene="pgi"
                     /locus_tag="VFA14_06335"
     CDS             1560..>2672
                     /gene="pgi"
                     /locus_tag="VFA14_06335"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008113623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HZS81026.1"
                     /translation="MPYTALTATAAFRALCSHHTDVRERHMRDLFAADARRFDRMNTK
                     AAGILLDYSKNRIDDTTLKLLFELARERGVETQRAAMFRGDKINFTEHRAVLHTALRA
                     PRGQGLLVDGQNIDANVHAVLERMHAFVERVRDGQWLGRSGRPITDIVNIGIGGSHLG
                     PQMACTALRAFSHPRLKMHFVSNVDGHDLDAVLQRIDADTTLFIVASKTFTTQETMIN
                     ARSARGWFLTRADEADLARHFIAVSTSTAAVRAFGIDPDNMFPFWDWVGGRYSMWSSI
                     GLSIALAVGFRHFLELLAGAHAMDQHFCAAPLERNMPAILALIGIWNRNFFNSAALSI
                     AAYHHDLRHFSAYLQQLDMESNGKHITRAGSAVSVAT"
BASE COUNT          548 a          800 c          779 g          545 t
ORIGIN      
        1 tgaaagcttc ctgtgaatgg aagacgatgg ccggcgcccg tactgcgcga tgcgcttgct
       61 ggactgcgga aaccttgatg accgagcgcc ccaggttgcc ttgcagcagc ttgagtccgc
      121 catcagccga gaatggattg gtcgccggac gcaatacggt ttcatcgccg ctgttctgcg
      181 gcgcctcttt ccagactgcc ctgccatctt ccagcagcgg ctcggcgcag tgggcgcgca
      241 agccgtgacc gagaatggtc atgacgtcgt catgcatcag accggcatcg agcaattcgc
      301 gcagaacgaa accagcgccg cctgcggcgt ggaagtggtt gacgtcggcg ctgccgttcg
      361 gatagatgcg cgtgatcagc ggcgccactg atgacagtct ggagaaatcg tcccagtcga
      421 tcaccagacc ggcagccttg gcgatcgcca ccaggtgcag tgtgtgattg gtggaaccgc
      481 cggtcgccag taaggcaacg atggcgttaa caatggattt ttcatcgacc aagcggccaa
      541 ccggcgtgta ttgtgtgcct tgcgcggtga tcttgagcgc ttgcctggcc gcagcagcgg
      601 tcagtgcatc gcgcagcggc gtatttggcg tgacgaaagc ggatcccggc agatgcaggc
      661 ccagcgcttc catcagcatc tggttgctgt tggcagtgcc gtaaaaggtg caggtgccag
      721 cgtcgtgata agactgcgct tcggcctcca gcaactcggc gcgaccgatc ttgccttcgg
      781 cgtagcgctg acggatcttg agtttttcgc tgttgctcat gccactggtc atcggaccgg
      841 caggaacaaa tactgcagga atgtggccga aatgcagggc gccaatcagc agacccggca
      901 cgatcttgtc gcacacgccc aggtacagcg cgctatcgaa catattgtgc gataacgcga
      961 ttgccgtacc catggcgata gtgtcgcgcg agaacagcga cagctccata ccggtctggc
     1021 cttgcgtaat accatcgcac atggccggca cgccgccggc gaattgcgcc acgccgccgt
     1081 ctgcgcggat ggcatccctg atgatgcgcg gaaaaccgtc gaaaggttga tgcgccgaca
     1141 gcatgtcatt gtaggacgag acgatggcca ccgacggcgc gcggtcctgt ttgagtttca
     1201 gtttgtcatt ggcagtgaag gcggcaaagc catgcgccag attggtgcat gacagcgcgc
     1261 tgcgctgcac gccgccatga cgcgccgcat caaggcgcgc gagataggcg gaacgataag
     1321 gccgactgcg ttcggcaata cggcgcgtga cactggccag ggtagcgtgg agagacatgc
     1381 cgggttcctg atgacgttag aaaactgtaa ttttattaca aaacatcaag caaaacatct
     1441 tcgttcacct gtttctattt catagtgttt tacccattta tactgtagtg aaattaccat
     1501 aaaatcagta aatcatgtat attgcttcct attttcaacc tcggccggga atgcagaata
     1561 tgccgtacac agcactcaca gcgaccgcag ctttccgggc gctgtgttcc caccataccg
     1621 acgtgcgcga gcgtcacatg cgtgacctgt ttgccgccga cgcccggcgc ttcgatcgca
     1681 tgaacaccaa agctgcgggt atcctgctgg attactccaa gaatcgcatc gacgatacca
     1741 cgctcaagct gctgtttgag ttagcccgcg aacgcggcgt cgaaacacag cgcgccgcca
     1801 tgttcagggg cgacaaaatc aatttcaccg aacatcgcgc tgtcctgcac acggccttgc
     1861 gcgcgccgcg cgggcaaggc ctgctggtcg atggacagaa catcgacgcc aacgtacatg
     1921 ccgtcctgga gcgcatgcat gccttcgtgg agcgcgtgcg cgacggccaa tggctgggac
     1981 gcagtggcag gccgatcacc gatatcgtca atatcggcat cggcggctca catcttggtc
     2041 cgcaaatggc ctgcaccgcg ttacgcgcct tcagccatcc acgcctgaag atgcacttcg
     2101 tctccaatgt cgatggtcat gacctcgatg cagtgctgca acggatcgat gctgacacca
     2161 ccctgttcat cgttgcctcc aaaaccttca ccacgcagga aaccatgatc aatgcgcgat
     2221 cggcgcgcgg ctggtttctc acgcgcgcgg acgaagcgga tctggcccgg catttcatcg
     2281 cagtatcgac cagcaccgct gcggtgcgcg ccttcggcat cgatcccgat aatatgtttc
     2341 cattctggga ctgggtcggc ggacgctatt cgatgtggtc gtcgatcggt ctgtcgattg
     2401 cgctggcggt cggcttccgg catttccttg aattgctggc cggcgcgcat gccatggacc
     2461 agcatttctg cgcagcaccg ctggagcgga acatgcctgc catcctggcg ctgatcggca
     2521 tctggaaccg caatttcttc aacagcgcag cgctgtcgat cgcagcgtat catcacgatc
     2581 tgcgtcattt ctctgcttac ctgcaacaac tggacatgga aagcaacggc aaacacatca
     2641 cacgcgccgg cagcgccgtc agcgtggcga cc
//
LOCUS       DASRDH010000272         2356 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_688067,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000272 DASRDH010000000
VERSION     DASRDH010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_688067"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="VFA14_06340"
     CDS             complement(<1..411)
                     /locus_tag="VFA14_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZS81027.1"
                     /translation="MTLTELRYIVAVAKKKHFGHAAEACFVAQPTLSVAIKKLEDELG
                     VTIFERGGTEVSVTPLGAQIVAQAERVLEQTATIKEIASQNKDPLSGPLRLGIIYTVG
                     PYLLPSLVKTMIRDVPQMPLILQENFTMHLVELLR"
     gene            613..1281
                     /locus_tag="VFA14_06345"
     CDS             613..1281
                     /locus_tag="VFA14_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008334189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HZS81028.1"
                     /translation="MNRTFSSLLKFLCITMLAVGSAAFAIEERKTGSAVKTDPVKGEA
                     LYTNGDAARNITACVSCHGVAGNSTISINPHLAGQHTAYVIKQLHNFRSASHNNPVMS
                     PLAKALSDEDVTNIAAYLNAQTPKPGAARNKDTLELGRHIWRGGIAFKNVPACAGCHG
                     PNGAGIPAQYPRLAGQHQDYTVAGLSSFRSGVRTNSPQMTMIAERLSDREIKALADYI
                     AGLK"
     gene            1490..2356
                     /gene="htpG"
                     /locus_tag="VFA14_06350"
                     /pseudo
     CDS             1490..2356
                     /gene="htpG"
                     /locus_tag="VFA14_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078561.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
BASE COUNT          576 a          690 c          614 g          476 t
ORIGIN      
        1 acgtagcagc tcgaccaggt gcatggtgaa attttcctgc aggatcagcg gcatctgcgg
       61 tacgtccctg atcatggtct tgaccagcga cggcaataaa taagggccga cggtgtagat
      121 gatgcccagg cgcagcggac ccgatagcgg atccttgttc tggctggcga tttccttgat
      181 agtagcagtc tgctccagca cgcgttcggc ctgcgctaca atctgcgcac ctagcggagt
      241 gactgacact tcagtgccac cgcgctcgaa gatggtgacg ccaagttcgt cttccagctt
      301 tttgatcgct accgacaagg tcggctgtgc tacgaaacag gcttcggcgg catggccgaa
      361 atgctttttc tttgcaactg cgacgatgta cctgagttcg gtgagtgtca tggacggtat
      421 ggcgttatgc gaatggatat gcgcagacta taacgcatct gcaagtgcta tgcagggcgg
      481 cggcatccag cctacgtgct gcgcctgaaa agtcattgta gaataagaag gtttctgaaa
      541 aaacagggga gggggaggag tcatcgccag tctggcatat ttattaccgc actcaaatct
      601 ctgggtattt gaatgaatcg taccttctca tcattgttga aattcttgtg cattaccatg
      661 ctggcggttg gttccgccgc atttgccatc gaagaaagga aaaccggatc tgctgtcaaa
      721 acagacccgg tcaagggcga agcgctttat accaacggcg atgctgcgcg taatattacc
      781 gcctgcgtgt cctgccacgg cgtagcgggc aactccacca ttagcatcaa tccacatctg
      841 gccggccagc acaccgctta tgtgatcaaa cagctacaca atttccgttc cgcaagtcac
      901 aacaaccccg tcatgtcgcc actggctaag gctttatcgg atgaagacgt gacgaacatc
      961 gccgcttacc tcaacgcgca gacgccaaag ccgggcgcgg ccagaaacaa ggataccctg
     1021 gagctgggca ggcacatctg gcgcggcggc atcgccttca aaaatgtacc ggcttgcgca
     1081 ggttgccacg gcccgaacgg cgccggcatc cctgcccagt atccacgctt agccggccag
     1141 catcaggatt acacagtcgc cgggctgagc agtttccgca gcggcgtccg taccaacagc
     1201 ccgcagatga ccatgatcgc ggagcgtttg tcagacaggg agatcaaagc attggcagat
     1261 tacatcgctg gtctgaagta aaaataccta gcccaacagc ccacgccggg cctgacccca
     1321 tgtctttatg catcagtcgg tcaagccgct tccagtcttt ccgatgttgc gcaagtcttg
     1381 aaaatcgtcc aagaatccgc attctcctta cagttggcgg cgcgcgaatc tatgccggca
     1441 gccacgttcc ctattcaaat aatcgttcca actcaggagg taaaacaaca tggcggtact
     1501 cgagaaacag accctgggat tccaggccga agtcaaacaa ctgctgcaac tgatgattca
     1561 ctccttgtaa tccaacaagg aaatcttcct gcgtgaattg atctccaatg cttccgacgc
     1621 agccgacaaa cttcgcttcg aagcgatcaa cgacagcgcc ctgttcgaaa acgatcccga
     1681 cctgaagatc aaggtcagct tcgacaaggc ggcgcgtacc atcaccatct ccgacaacgg
     1741 catcggcatg aaccgtgacg aagcgatttc ccatctgggt acgatcgcca agtccggcac
     1801 caaggaattt ttttcgaaac tgtccggcga ccagcaaaag gatgcggcgc tgatcggcca
     1861 attcggcgtc ggcttctact ccggcttcat cgtcgccgac cgcatcacgg tcgaaacccg
     1921 ccgcgccggc accccggcca gtgcaggcgt gcgctgggaa tccgaaggcg ccggcgaatt
     1981 ctcgatcgaa gcaatgacca agcctggccg cggcaccgac atcattttgc acctgcgcga
     2041 aggcgaagat gatttcctgt cgtcatggca gctcaagaac atcatccgca agtattccga
     2101 ccacatctcc ctgccgatcc tgatgcagaa ggaagcatgg gatgaagaaa aaaaagaaac
     2161 catcctcaag gatgaactgg aaaccgtcaa ccaggccagc gccttgtgga cgcgcagcaa
     2221 ggccgacatc acgccggaac aatacgaaga gttctacaag catatctcgc acgatttcag
     2281 cccgccgctg gcctatacgc acaaccgcgt cgaaggccgt agcgaatata cccagttgct
     2341 gtacatcccg acgcgt
//
LOCUS       DASRDH010000273         8410 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1205325,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000273 DASRDH010000000
VERSION     DASRDH010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 8410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8410
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1205325"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1067
                     /locus_tag="VFA14_06355"
     CDS             <1..1067
                     /locus_tag="VFA14_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="porin"
                     /protein_id="HZS81029.1"
                     /translation="MNNGNWFTDTKRNVAMNSGILQGSRLGFKGTEDLGGGLSAVFNL
                     EAGFKNDTGALDENGVLFRRKSVVGLSGGFGTVLLGRQTDVLDDIGGTLTSVADFGGV
                     TGAVGHNLDRLEGTRSNNSIRYNTANLGGFVGTLMYGLGEQAGKNSAGQSFGVGGLYT
                     NGPLGLGAGYYQSKAGATPSDASLVSSIGPDGEDGATVLKTFTLAASYQLGAARLYGN
                     WSRVKQPTAAAVDGDTVLATYNSNGTWSPKVFTVGGSNNDKTDIFELGTAYALNPSLK
                     LLGSVQQSRINYVDAAKGKLTQINLGADYWLSKRTDLYAFVSNLRANDGQLFNPGISN
                     NTASDQTSQTAVSVGIRHKF"
     gene            1260..1928
                     /locus_tag="VFA14_06360"
     CDS             1260..1928
                     /locus_tag="VFA14_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013236221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HZS81030.1"
                     /translation="MTTHLDSDSIADYLLDHPRFFEKHAELLSLIRLTSPLLGRTVSL
                     QERQIEILREKIKVQELHMADLLRVAQKNDEITSKFLAWTRALLLARNDADLPRILTD
                     HLQKLFDVPQVTVRLWNIAERHHGAWFNAAIPENARIFANGLSTPFCGASSDFEADVW
                     LEDPVSVKSVALLPLRVDAALEAFGLLVLGSPDPDRFTAEMGTSFLGRISETASAALA
                     CLLR"
     gene            1995..2957
                     /locus_tag="VFA14_06365"
     CDS             1995..2957
                     /locus_tag="VFA14_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HZS81031.1"
                     /translation="MSANIDQAEFARYLDSLQNQRRLSSHTISSYARDLGELSALARA
                     ANVALDAVAHFQIRQFASQLHGKGLGARSIARKLSAWRGFYDWLSEQGGLTSNPVDGI
                     KAPKKAQALPKALAVDDAVYLVSHTNPLKDADSPAQLCNRAMFELLYSSGLRVSELIA
                     LDLQYHADYASAGWVDMHGGEVTVTGKGNRMRSVPVGRPAIQALATWLAARASIAAKH
                     TGADPHALFLSARGTRMSARVAQLRLEAHGQALGMATSVHPHVLRHSFASHVLQGSGD
                     LRAVQEMLGHTSITTTQIYTSLDFQRLAQVYDAAHPRAKKVTRK"
     gene            3032..3595
                     /locus_tag="VFA14_06370"
     CDS             3032..3595
                     /locus_tag="VFA14_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007879380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HZS81032.1"
                     /translation="MNSRPAASTTSSVDAHDPAKAQALARLQLRAAAVRVTQARVSVM
                     ALLLSARSAVSHQDIQDQLVGMDRVTLYRVLDCLTDAGLAHKIASDDRIFRYNAGAGK
                     EHHDAPQSAQGHPQHGHFRCMRCARVFCLDGSGEAGFLESVLPSSGKRRSTTAQLQSA
                     LQKSLGKGFQSHEIELTVKGWCDDCAG"
     gene            complement(3597..4511)
                     /gene="rsmI"
                     /locus_tag="VFA14_06375"
     CDS             complement(3597..4511)
                     /gene="rsmI"
                     /locus_tag="VFA14_06375"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007878949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HZS81033.1"
                     /translation="MSHPDSNAPIASIASSILTEASQQTYPGSALYVLATPIGNVCDM
                     TLRALHVLTIANAVACEDTRNTSHLLTRYGISQTLIAAHQHNEREAAGKIIARLQQGE
                     RIALVSDAGTPAVSDPGARIVAAVRAAGLTVVPLPGASAAVAALSVSGLVNDTFYFIG
                     FLPSKSRQRENVLLKHARDSATLVFYEAPHRIVDTVDALLQAFGPQRQIVLARELTKL
                     FETIHCCALVDAPAWLAADLNRQRGEFVILVEGAPADADTDTDAERVLQILLAELPLK
                     QAAALAAQITGQKKSVFYERALQIRDSR"
     gene            4550..5686
                     /locus_tag="VFA14_06380"
     CDS             4550..5686
                     /locus_tag="VFA14_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007878951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HZS81034.1"
                     /translation="MLTALLGGLCASIQANTSSAGNSMLDLNADGHRPLVRVALLLPL
                     RSSVLGAAADAVRRGFLAAAEQKRSNIVVTIIQTGDASAEVLGVYHDASAKYDIIVGP
                     LSRSAVTALAQSSAVSKPTIALTQPDLPDIPDRRLPSRMIMMGLSVEEEARQVATWVE
                     REKPPGKIFAVSTNMTWQRRAVKAFVQQARKIGMTVDNLEFSGSSHTLGASDLMQLNR
                     RIQAEKPALLFVALDATQTIQLRKVIGGDVPLYGTSQLNPLAMTTAAGAPPVNRLPEL
                     NGVYLLDLPWQLQANHPTVMAYPRAIAKTGSPPNADLERLYGLGIDAFRVADEIARQR
                     SDFDIHGVTGQLYVNMAVGAAHFQRKLIQAVYQDGVTIPVSDQR"
     gene            5693..6070
                     /locus_tag="VFA14_06385"
     CDS             5693..6070
                     /locus_tag="VFA14_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HZS81035.1"
                     /translation="MALFDRLRGARTSRQISGDAAEGAALAFLLQQGLHEVQRNFRCK
                     GGEIDLIMRDDDVVVFVEVRKRSKDSYGGALASVTRAKQKRLIIAAQVFLQSYRCLPA
                     CRFDVLAYDGDRLTWLKNAVEIY"
     gene            6173..6769
                     /locus_tag="VFA14_06390"
     CDS             6173..6769
                     /locus_tag="VFA14_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HZS81036.1"
                     /translation="MTNQRILSHFQESATLKMHAGAVLAPPIEQAIELMFTALSNGNR
                     ILACGNGGSAADCQHFAAELVGRFERERLPLPALALTTDTSILTAIGNDYGYQEIFSK
                     QVQAFGQSGDVLLAFSTSGNSGNVLAAIEVAQERDMRVVALTGKGGGVIGSKLTDADV
                     HICVPHDRTARIQEVHLVILHCICDGIDVALFGGDVNE"
     gene            6762..7412
                     /locus_tag="VFA14_06395"
     CDS             6762..7412
                     /locus_tag="VFA14_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008333582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HZS81037.1"
                     /translation="MSNIKPECLKWHRPLAITAVCGLLAIVLQGCFGMLAGGMLAGTF
                     AATDRRTLGAQVEDKTIVIKGEASISGVVPAGSHVNVTSFNRKVLLSGEVPDEASRVT
                     AGRKITDLPNVDSVFNELVIAGSSSFTSRSSDTLITSKVLASLIDAKDIYANAFKITT
                     ERGIVYMMGRVTEREGRRGAHIASGVSGVQKVVTLYEYISEEELRQYTQQPANSAG"
     gene            7431..7976
                     /locus_tag="VFA14_06400"
     CDS             7431..7976
                     /locus_tag="VFA14_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016833803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HZS81038.1"
                     /translation="MKAQNAITTPIVGTLRNRFWIKPGGLLIIAALTLFGYIALSAHN
                     TAPDVTFVKLDGQKIALRDLRGKVVIVNFWATSCTTCIGEMPQMTATYDKYRDQGLDF
                     VAVAMSHDPPHYVLNYVRTRRLPFQVALDPQDTLAKAFGDVKLTPTTFVIGKDGAILK
                     RYVGKLQFAEFHQLLEKALKV"
     gene            complement(8029..>8410)
                     /locus_tag="VFA14_06405"
     CDS             complement(8029..>8410)
                     /locus_tag="VFA14_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011617110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HZS81039.1"
                     /translation="WNHPDTPMLYCASSIALACLETMVHIAAGSLPLNRYLVRIDVPD
                     DVWDGAQTLTAASAPAGWDALPEGKVSLDYGTQWAMSKSTALLCVPSVVVPEEWNVLV
                     NPKHADAARVTATKLRKWIYDARI"
BASE COUNT         1796 a         2537 c         2388 g         1689 t
ORIGIN      
        1 ccatgaacaa cggcaactgg tttaccgaca ccaagcgcaa cgttgcaatg aattccggca
       61 tcctgcaagg ttcgcgcctt gggttcaagg gcactgaaga tctgggcggc ggtctctccg
      121 ccgtctttaa tctggaagcc ggcttcaaaa acgacactgg cgccctagat gaaaacggcg
      181 ttctgttccg tcgcaagtcg gtcgttggtc tgtcgggcgg tttcggtact gttttgctgg
      241 gccgtcagac cgacgttctg gacgatatcg gcggcaccct gacttcggtc gctgatttcg
      301 gcggtgtcac cggcgcagtc ggccacaatc tggatcgtct ggaaggtacg cgttccaaca
      361 actcgatccg ctataataca gcgaacctgg gtggcttcgt tggtaccttg atgtacggtc
      421 tcggtgaaca agctggtaaa aattctgcag gtcaatcgtt tggtgtcggc ggcctgtaca
      481 ccaacggtcc gctgggtctg ggtgctggct actaccagtc caaggccggc gcgactccca
      541 gcgatgcttc cctggtcagc tccatcggtc ccgacggtga ggacggcgcg acggtcctga
      601 agacgttcac actggctgct tcctatcagc tgggcgccgc ccgtctgtat ggtaactggt
      661 cccgcgtcaa gcaaccgaca gccgctgcag ttgacggaga cacggttctg gctacctaca
      721 acagcaacgg cacatggtct cccaaggtgt ttaccgtcgg cggcagcaac aacgacaaga
      781 ccgacatctt tgaactgggt acggcctatg ccctgaatcc atcgctgaaa ctgctgggca
      841 gcgttcaaca atcccgtatt aattatgttg atgcagcaaa aggcaagttg acccagatca
      901 acctgggcgc tgactactgg ctgtcgaagc gtactgatct gtacgcattc gtgtcgaacc
      961 tgcgtgccaa cgacggccaa ctcttcaatc caggtatcag caacaatact gccagcgatc
     1021 agaccagtca aacagcagtg agcgttggta tccgccacaa gttctgattc aggcttcact
     1081 tccggcatgt caatgtcgga aaaccagatg cctgcttgaa gattcgggct tgcatgagca
     1141 atcatgcaag cccgttttta ctggaaaatg cctgtgtcgg catccggacg aaaaagttgt
     1201 cagcatcggc gatagcggat aatctcgtga tattgcgaac agatagacga ctctgcccta
     1261 tgaccaccca tctggactcc gattcgatcg ccgactatct gctcgaccat ccgcgcttct
     1321 ttgagaaaca tgccgagttg ctgagtctga tcaggctcac cagcccgtta ctggggcgta
     1381 ccgtctcctt gcaggagcgt cagatagaaa tcctgcgcga aaagatcaag gtgcaggagt
     1441 tgcacatggc cgacttgcta cgtgtcgcgc aaaaaaacga cgagatcacc agcaaatttc
     1501 tggcatggac acgcgcactg ctactggccc gcaatgacgc tgatttgcca aggatattga
     1561 ccgatcatct acagaagctc ttcgatgttc cgcaagtcac cgtgcgtcta tggaatatcg
     1621 ccgaacgcca tcacggcgcc tggttcaatg ccgccatccc ggaaaatgcg cgtatcttcg
     1681 ccaatggcct gagcacaccg ttctgcggcg ccagcagcga cttcgaagcc gacgtctggc
     1741 tggaagaccc ggtctccgtg aaatccgtgg ccctgctgcc gttgcgcgtg gacgctgcgc
     1801 tggaagcctt cggcctgctg gttctcggct cacccgatcc ggaccgcttc acggcggaaa
     1861 tgggcaccag tttcctgggg cgtatcagcg aaaccgccag tgctgcgctg gcttgcctgc
     1921 tgcgctaaac gccgctaaca aaacgtcgat ggcaaactta gcggctctca attccgacca
     1981 cgattcccga ccgtatgagc gccaacatcg atcaagcaga atttgcccgt tacctcgaca
     2041 gcctgcaaaa ccagcgcagg ctgtcgtcac acacgatcag cagctatgcc cgcgatctcg
     2101 gtgaattgag cgcgctggcg cgagctgcca acgttgccct cgatgcggtt gctcactttc
     2161 agatccgtca atttgcctcc cagctgcatg gaaagggtct cggcgcccgt tccatcgccc
     2221 gcaaactttc ggcatggcgt ggcttctacg actggctgtc cgaacaaggc ggcctgacat
     2281 ccaatcccgt tgatggcatc aaggcgccca aaaaggccca ggctttgccc aaagcgctgg
     2341 cagtggacga tgccgtctat ctggtatcgc acaccaaccc gctcaaagat gccgactctc
     2401 cggcacaact gtgcaaccgg gccatgttcg aactgctgta ttccagcgga ctgcgcgtgt
     2461 ccgaactgat cgcgctggac ctgcaatacc acgccgacta tgcttcagcc ggctgggtcg
     2521 atatgcacgg tggtgaagtg accgtcactg gcaaaggtaa caggatgcgc agcgtgccgg
     2581 tcggtcggcc ggccatacag gcgctggcaa catggctggc cgcgcgtgcc agtatcgctg
     2641 caaaacatac cggcgccgat ccacacgccc tgtttctgag cgcacgcggc acgcgtatgt
     2701 cagcgcgcgt ggcgcaatta cgactcgagg cccacgggca agcgctgggc atggcgacca
     2761 gtgtgcatcc tcacgtgctg cgccattcct tcgcttctca cgtgctgcaa ggctcggggg
     2821 atttacgcgc agtccaggag atgctgggcc acacttccat tacgacgacc cagatctata
     2881 cttcgctcga cttccagcgc ctagcgcagg tctatgacgc cgcccatccc cgcgccaaga
     2941 aagtcacccg gaaatagaat cacgcaggca cagacgcaaa accttgcatc gcgaattgcg
     3001 gcataatcca tacagtacct gcaaatacac catgaactcc agaccagccg caagcaccac
     3061 atcaagcgtt gatgcgcatg atccagccaa ggcacaggca ttggcgaggc tgcaactgcg
     3121 cgctgccgcg gtgcgcgtga cgcaggcgcg cgtgagcgtc atggcgctgc tgctcagcgc
     3181 acgcagcgcg gtctcgcatc aggatattca ggatcaactg gtcggcatgg atcgcgtcac
     3241 cctgtaccgt gtcctggatt gcctgaccga cgcgggactg gcgcacaaga tcgccagcga
     3301 cgaccgcatc tttcgttaca acgccggcgc cggcaaagaa catcatgacg cacctcagtc
     3361 tgcgcagggc catccccagc atggccattt cagatgtatg cgctgtgccc gcgtgttttg
     3421 cctcgacggc agcggtgaag caggttttct ggaaagcgtt ttaccctcat ccggcaagcg
     3481 ccggagtacc acggcgcagt tgcagagcgc gctgcaaaaa tccctcggca aaggttttca
     3541 aagccacgaa atcgaattga ccgtcaaagg ttggtgcgat gattgcgccg gttagctcat
     3601 cgggagtccc tgatctgcaa agcgcgctca tagaacacgc ttttcttttg tccggtgatt
     3661 tgcgctgcca gcgccgcagc ctgtttgagc ggtagttctg ccagcaatat ctgcaacacg
     3721 cgttcggcat cagtatcagt atcggcatcg gcaggcgcgc cctccaccag aatcacgaat
     3781 tcgccgcgct gccggttgag gtccgccgcc agccaggccg gcgcatcaac caacgcgcag
     3841 caatgaatgg tctcgaacag tttggtcagc tcgcgtgcga gtacaatctg acgctggggg
     3901 ccaaaggcct gcaacagcgc atcgacggta tcgacgatgc ggtgtggcgc ttcataaaac
     3961 accagcgtcg cgctgtcacg cgcgtgtttg agcaagacgt tttcgcgctg cctgcttttg
     4021 ctcggcaaaa agccgatgaa atagaacgta tcattgacca gaccactgac agatagcgct
     4081 gccaccgccg ccgatgcgcc cggcaagggc accacggtca ggcccgcagc gcgcaccgct
     4141 gcgacaatgc gcgcgcccgg atccgagact gccggagtac cggcatcgga caccagagcg
     4201 atgcgctcgc cttgctgcag acgtgcaatg atcttgccgg cggcttcacg ctcattgtgt
     4261 tggtgcgcgg caatcaaggt ctggctgata ccatagcggg tcagcaaatg gctggtgttg
     4321 cgcgtgtctt cgcaagccac ggcattggcg atcgtgagga catgcaaagc gcgcaacgtc
     4381 atgtcacaaa catttccgat gggagtagcc agcacatata atgccgatcc agggtaggtt
     4441 tgctgtgagg cttcggtcag gatggacgac gcaatggatg caatgggcgc gttggaatca
     4501 ggatggctca tgggagaaaa cgatgtcggg aaaatggacg aaaatcatca tgctgaccgc
     4561 gctgctgggc ggattgtgcg cttccataca ggcaaacaca agctctgccg gcaatagcat
     4621 gctcgacctg aacgccgacg gccatcgtcc gcttgtgcgt gtcgccttgc tgctgccctt
     4681 gcgttccagt gtgctcggcg cggcggctga tgccgtgcgc agaggttttc tggctgctgc
     4741 cgagcagaaa cggagcaaca tcgtcgtcac cattatccaa accggcgatg ccagcgcgga
     4801 agtgcttggc gtctaccatg atgcctctgc caaatatgac atcatcgtcg gtccgctctc
     4861 gcgcagcgca gtgaccgccc tggcgcagtc cagcgccgtg agcaagccga ccatcgccct
     4921 gacgcaaccg gatctgcccg atatccctga tcgacgcctg ccatcccgga tgatcatgat
     4981 gggtttgtcg gtcgaagaag aagcgcgcca ggttgcgacc tgggtcgagc gtgaaaagcc
     5041 accgggcaag atcttcgccg tttccaccaa catgacgtgg cagcgccgtg ccgtcaaagc
     5101 ctttgtacag caagcgcgga aaatcggcat gacggtggac aatctcgagt tcagcggctc
     5161 cagtcatacg cttggcgcca gcgatctgat gcaattgaac cggcgcattc aggccgagaa
     5221 accagccttg ctgtttgttg ctctcgatgc gacacagacc attcagttgc gcaaagtcat
     5281 cggcggcgac gttccgctat acggcacctc acagctcaat ccgctggcga tgacaaccgc
     5341 tgccggcgca ccacccgtca atcgcctgcc cgaactcaat ggtgtgtatc tgcttgacct
     5401 gccttggcaa ttgcaggcga atcatccgac cgtgatggcg tatccacgcg ctatcgccaa
     5461 gactggcagc cctccgaacg ccgatctgga acgcctgtat ggactcggca tcgatgcctt
     5521 tcgcgtggcc gacgagatcg cccggcagcg ctccgatttc gacatccatg gcgtaaccgg
     5581 ccagctctat gtcaacatgg cagttggcgc cgctcatttc cagcgcaaac tcatccaggc
     5641 agtctatcag gacggcgtca ccattccggt gagcgaccag cgctgaaccg gcatggccct
     5701 tttcgaccgg ctgcgcggcg cacgtaccag cagacaaatc agtggcgatg cggccgaggg
     5761 tgcagcgctg gcatttctgc tgcaacaagg tctgcatgag gtacagcgca atttccgctg
     5821 caagggaggc gagatcgatc tcatcatgcg cgatgacgat gtcgtcgttt tcgtcgaggt
     5881 gcgcaaacgc agcaaagaca gttacggcgg cgcgcttgcc agcgtcacgc gcgcgaaaca
     5941 gaaacgcctg atcattgccg cccaggtatt cctgcaaagc tatcgctgct tgcctgcctg
     6001 ccgtttcgac gtactggctt acgacggcga ccgcttgacc tggctgaaaa atgctgtgga
     6061 aatctattga gatgtcccct gaccgcctgc gcgcgggcgc ggttgcgcaa ctacactata
     6121 attggacgcc caactaggat gttcttcatg catcgtcaac tacgctcgca ccatgactaa
     6181 tcaacgtatc ctttcacact tccaggaaag cgccacactc aagatgcatg ccggcgcggt
     6241 gctggcgccg ccgatcgagc aggcgattga actcatgttc acggcattgt cgaacggtaa
     6301 caggattctt gcctgcggca atggcggttc agcagctgac tgccagcatt tcgccgcgga
     6361 actggtagga cgtttcgaac gcgagcgttt gcctctgccg gcgctggccc tgacaacgga
     6421 tacgtcgatc ctgacagcca tcggcaatga ctatggttac caggagattt tttccaagca
     6481 ggtgcaggcc tttggccaga gcggcgacgt gttgctggcg ttctcgacct cgggcaattc
     6541 cggcaatgtg ctggcggcga tcgaggttgc gcaggaacgc gacatgcgcg tggtggcact
     6601 gaccggcaaa ggcggcggcg tcatcggcag caaactgacc gacgccgatg tccatatctg
     6661 cgtgccgcat gatcgcaccg cgcgcattca ggaggtgcat ctggtgattt tgcattgtat
     6721 ttgcgacggt atcgacgttg cattatttgg aggcgatgtc aatgagtaat atcaaaccgg
     6781 aatgcctgaa atggcatcgt ccgctggcca tcaccgccgt ctgcggcctg ctcgcgatcg
     6841 tgctgcaggg ttgcttcggc atgttggccg gcggtatgct ggcaggcacg tttgcggcca
     6901 cggatcggcg cacgctgggc gcgcaggtgg aagacaaaac gattgtcatc aagggggaag
     6961 ccagtatttc aggcgtcgtg ccggcaggtt cccacgtcaa cgtaaccagt ttcaaccgta
     7021 aagtcctgct gagcggcgaa gtgcctgatg aagcttcccg ggttaccgcc ggacgtaaaa
     7081 tcacggactt gccaaacgtc gacagcgtgt tcaatgaact ggtcattgcc ggttcatcca
     7141 gcttcacctc acgttccagc gacacgctga tcaccagcaa agttctggcc agcctgatcg
     7201 atgccaagga tatatacgcc aatgccttca agatcacgac cgaacgcggc atcgtctaca
     7261 tgatgggacg cgtgaccgaa cgtgaaggca ggcgcggtgc ccatatcgcc agcggcgtga
     7321 gcggcgtgca aaaggtcgtg acactgtacg aatacatctc ggaagaggaa ctccggcaat
     7381 atacccaaca accggcgaat tccgccggat aatcgcgcgc cgcgcaaaac atgaaagccc
     7441 aaaacgccat caccaccccc atcgtcggga cactgcgcaa ccgcttctgg atcaaacctg
     7501 gcgggctgct catcattgca gcgctcaccc tgttcggcta tatcgcactg tcagcacata
     7561 acacggctcc tgatgtgact ttcgtcaaac tcgacggaca aaagatagcg ctcagggatt
     7621 tgcgcggcaa ggtcgtgata gtcaatttct gggcgaccag ctgcaccact tgcatcggcg
     7681 aaatgccgca aatgactgcg acctatgaca agtatcggga tcaggggctg gatttcgtcg
     7741 ccgtggcgat gagccacgat ccgccgcatt acgtcctcaa ctatgtccgg acgcgcaggc
     7801 tgcccttcca ggttgcgctc gacccacaag acacactggc caaagcgttc ggtgacgtca
     7861 agctgacacc gactaccttc gtgatcggca aggacggcgc tattttgaaa cgctacgtcg
     7921 gcaagctaca gtttgccgag tttcatcagc tgctggagaa ggcgctgaaa gtataaccgt
     7981 gagtagtccg gcatgcagtc aggctcacac aggcggcgcg catcatgcct atatccgcgc
     8041 gtcatagatc cacttacgca gcttcgtggc cgtcactctc gcggcgtctg catgtttggg
     8101 attgacgaga acgttccatt cttcgggtac aaccacagac ggcacgcaaa gtagcgcggt
     8161 gctttttgac atggcccatt gcgtaccgta atcgagactc accttgccct cgggcagcgc
     8221 gtcccatcca gccggcgccg atgccgccgt caaggtctga gcgccgtccc aaacatcatc
     8281 gggcacatcg atcctcacca gatagcgatt cagcggcagg gagccggcgg cgatatgcac
     8341 catggtttcg agacacgcga gtgcgatgct cgaggcgcag tacaacatcg gtgtatccgg
     8401 atggttccag
//
LOCUS       DASRDH010000274         4274 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1122888,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000274 DASRDH010000000
VERSION     DASRDH010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 4274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4274
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1122888"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..681)
                     /gene="dapA"
                     /locus_tag="VFA14_06410"
     CDS             complement(<1..681)
                     /gene="dapA"
                     /locus_tag="VFA14_06410"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HZS81040.1"
                     /translation="MIKGSIVAIVTPMHPDGSLDFPGLRKLIDWHITEGSDAIVIAGT
                     TGESPTVSVEEHCELIRVAVEHTAKRIPIIAGTGSNSTSEAIELTDFARKVGVDASLQ
                     VVPYYNRPTQEGMYRHFRKIAESVDLPVILYNVPGRTVADMSNDTILRLVQVEGIVGV
                     KDATGDIGRGTDLIRLVPKSFAVYSGDDATAIALMLYGGRGCISVTANIAPHDMHELC
                     VAAMSGNIA"
     gene            complement(931..>4274)
                     /gene="rpoC"
                     /locus_tag="VFA14_06415"
     CDS             complement(931..>4274)
                     /gene="rpoC"
                     /locus_tag="VFA14_06415"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008334119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HZS81041.1"
                     /translation="QEAVDSLLDNGRRGKAMTGANKRPLKSLAEMIKGKGGRFRQNLL
                     GKRVDYSGRSVIVVGPQLKLHQCGLPKLMALELFKPFIFNKLELMGLATTLKAAKKLV
                     EIQEPVVWDILEEVIREHPVMLNRAPTLHRLGIQAFEPVLIEGKAIQLHPLVCAAFNA
                     DFDGDQMAVHVPLSIEAQMEARTLMLASNNILFPSNGEPSIVPSQDIVLGLYYATREA
                     INARNEGMIFPDVTEVIRAYDNKEVELATRITVRIVEHLKSAATGEFVRTVKRYETTV
                     GRAILSEILPKGMPFSALNRALKKKEISKLINTSFRKCGLRATVVFADQLMQSGFRLA
                     TRAGISICVDDMLVPPQKATIIATAESEVKQIEQQYSSGLVTAGERYNKVVDIWGKAG
                     DDVGRAMMDQLKVEDVIRRDNTKGTQESFNAIYMMADSGARGSAAQIRQLAGMRGLMA
                     KPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTRRLVDVTQD
                     LVVIEDDCGTSNGASMKALVEGGEVIEALRDRILGRVAATEIINPETQTTLYEAGTLM
                     DEDAVEEIERLGIDEVKVRTPLTCDTRYGLCAKCYGRDLGRGTLVNSGEAVGVVAAQS
                     IGEPGTQLTMRTFHIGGAASRAAVASSVEARSNGIVRFTAAMRYVTNSKGELIVISRS
                     GEVLITDDLGRERERHKALYGATLIVKDGMVVKAGTVLATWEPLTRPIITEYTGTVKF
                     ENVEEGSTVARQIDEVTGLSTLVVIDAKRRGSTVTKVLRPQVKLLNEHGEEVKIAGTE
                     HAVTIGFQVGALITVRDGQQVHVGEVLARIPTESQKTRDITGGLPRVAELFEARSPKD
                     AGMLAEVTGTVAFGKETKGKQRLEITDMDGVRHEFLITKDKQVLVHDGQVVNKGEMIV
                     DGPADPQDILRLLGIEALARYIVDEVQDVYRLQGVKINDKHIEVIVRQMLRRVQVVDS
                     GDTCYITGEQVERSELLDENDRVIAENKIPATYENVLLGITKASLSTDSFISAASFQE
                     TTRVLTEAAIMGKKDGLRGLKENVIVGRLIPAGTGLAFHRARKTKDAWEADERAALLE
                     SERQARVEETEVRSIEAFGSADGEA"
BASE COUNT          893 a         1282 c         1122 g          977 t
ORIGIN      
        1 ggcgatattg ccactcatgg cggcaacgca cagttcgtgc atgtcatgcg gggcgatatt
       61 ggcggtgaca gaaatacagc cacgaccgcc gtacagcatc aacgcaatcg ccgttgcatc
      121 gtcgccggaa tagactgcga acgatttcgg cactaggcga atcaggtccg taccgcggcc
      181 aatgtcgccg gtcgcgtcct tgacgccgac gatgccctcg acctgcacca gacgcaggat
      241 ggtgtcgttg ctcatatcgg ccacagtgcg gccgggaaca ttgtacagga ttaccggcaa
      301 gtcgaccgac tcggcgatct tcctgaaatg ccggtacatg ccttcctgcg tcgggcgatt
      361 gtagtaaggc acgacctgca gcgaggcatc gacgccgact ttcctggcaa agtctgtcaa
      421 ctcgatggct tccgacgtcg aattgctgcc tgtgccggcg atgatgggaa tgcgtttggc
      481 ggtatgctcg accgcgaccc tgatcagttc gcaatgttcc tcgactgaaa cagtcggcga
      541 ttcgccggtg gtgccggcga tcacgatagc gtcactgcct tctgtgatat gccagtcaat
      601 caatttgcgc aggccaggga aatcgagact gccatccgga tgcatcggcg tgacgatggc
      661 gactatgctg cccttgatca tgatgtgctt gcgtagtgag agggaaataa aaaaccgatt
      721 gtagcggata gctgctgcta tcggtcgatt tgttcaaatt cgcccattca gtatcattga
      781 tgcttagagc tatagccccg aattttcatg acccgctcca tcaaatgccg gaactaaata
      841 tccaggacac ataaaaacgg cgtcagaact tcttctgacg ccgtttttgt ttcgatagcc
      901 agtgaaggcg gttagcatac tactattaca tcacgcttcg ccgtccgcgc tgccaaatgc
      961 ctcgatggag cgcacttcgg tttcttccac acgcgcctga cgctcggatt ccagcagtgc
     1021 tgcgcgctcg tcggcctccc atgcatcttt tgtcttgcgg gcgcggtgga acgccagacc
     1081 agtaccagcc ggaatcaaac ggccaacgat gacattttcc ttcaggccgc gcagaccatc
     1141 cttcttgccc atgatcgccg cctcggtcag gacacgtgtc gtttcctgga acgatgcagc
     1201 cgagatgaac gagtcggtcg acagcgatgc cttggtgata cccagcagga cattttcgta
     1261 agttgcagga attttgttct ctgcaatcac acgatcgttt tcatccagca gttccgaacg
     1321 ctcgacctgc tcgccggtga tgtaacaagt atcacccgaa tccacaacct gcacacgacg
     1381 taacatctga cgcacgatga cttcgatgtg cttgtcgttg atcttcacgc cttgcaaacg
     1441 gtacacatcc tgtacttcat cgacgatgta acgcgccagc gcttcgatac ccaacagacg
     1501 caggatgtct tgcgggtcag caggaccatc cacgatcatt tcgcccttgt tcacgacctg
     1561 gccatcatgc accagcactt gcttgtcctt ggtgatcagg aattcatgcc tgacgccatc
     1621 catatcagtg atttccagac gttgcttgcc cttggtttcc ttaccgaacg ccaccgtacc
     1681 ggtgacttcg gccaacatac ccgcatcctt cggcgaacgt gcttcgaaca attcggcaac
     1741 acgcggcaga ccaccggtaa tatcacgggt cttttgtgat tcggtcggga tacgcgccag
     1801 cacttcgccg acgtgaacct gctgaccatc cctcaccgta atcagcgcgc cgacctggaa
     1861 gccgatcgtc actgcgtgct cagtaccggc gatcttgact tcttcgccgt gttcgttcaa
     1921 caacttgacc tggggacgca atactttggt aacggtcgaa ccacggcgct tggcgtcaat
     1981 gaccaccagg gtcgacaaac cggtcacttc gtcgatctgc ctggcaacag tcgagccttc
     2041 ttcgacattt tcgaatttca cggtaccggt gtattcggtg atgatcggac gtgtcaacgg
     2101 ctcccatgtc gccagtaccg tgccagcctt gacgaccatg ccatccttga cgatcagggt
     2161 cgcgccgtac agtgctttat gacgctcacg ctcacggcca agatcgtcgg taatcagcac
     2221 ttctcccgaa cgggaaatga cgatcagctc gcctttactg ttggtgacgt aacgcatggc
     2281 cgctgtaaag cggacaatcc cgttggacct ggcttcgacc gacgaggcca ctgctgcgcg
     2341 tgatgccgca ccaccgatgt ggaaggtacg catggtcaac tgggtgcctg gttcgccgat
     2401 cgactgcgca gcaaccacac cgacggcctc gccgctgttg accagcgtgc cgcggcccag
     2461 atcgcggccg tagcacttgg cgcacaaacc gtagcgtgta tcgcaggtca gaggtgtgcg
     2521 caccttgact tcatcgatgc cgagacgctc gatttcttcc acagcgtctt cgtccatcaa
     2581 ggtaccggct tcgtacagcg tagtctgggt ttccggattg atgatctcgg tcgctgcaac
     2641 acggccaagg atgcggtcgc gcagcgcttc gatcacttca ccaccctcga ccagcgcttt
     2701 catggaggcg ccgttggagg tgccacaatc gtcttcgatc acgaccagat cctgggtcac
     2761 gtcaaccaga cggcgtgtca aataaccgga gttcgcggtc ttcagcgcag tgtcggccag
     2821 acccttccgg gcgccgtgcg tcgagatgaa atactgcaac acattcaggc cttcgcggaa
     2881 gttcgcggta atcggcgttt cgatgatcga accgtctggt ttcgccatca gaccacgcat
     2941 accagccaac tgacgaatct gcgctgcgga gccacgggcg ccggagtcgg ccatcatata
     3001 aattgcgttg aacgactctt gtgtgccctt ggtgttatcg cggcggatca catcttccac
     3061 cttcagctgg tccatcatgg ccctgccgac atcatcaccg gctttacccc agatgtccac
     3121 caccttgtta taacgttctc cagcagtgac cagaccggac gagtattgct gttcgatctg
     3181 tttgacttcg ctttcggcgg tcgcaatgat ggttgccttt tgcggcggca ccaacatatc
     3241 atccacgcag atcgagatac cagcgcgcgt cgccagacga aagccagact gcatcagctg
     3301 gtcagcgaac accaccgtag cgcgcaggcc gcacttgcgg aacgatgtat tgatcagttt
     3361 tgaaatttct ttcttcttca acgcacgatt caatgcagag aatggcatcc ccttcggcaa
     3421 aatttccgac aggatggcac ggccgacggt cgtttcgtaa cgcttgacgg tacgcacgaa
     3481 ttcgccggtc gcagcactct tcagatgttc tacgatacgc acggtgatgc gggttgccag
     3541 ttcgacttcc ttgttgtcgt aagcacgaat gacttcggtc acgtcaggga agatcatgcc
     3601 ttcgttcctg gcgttgatcg cttcacgcgt agcgtagtac agacccagca cgatatcctg
     3661 ggatggcacg atcgatggtt caccgttcga tggaaacagg atattgttcg acgccagcat
     3721 cagtgtgcgc gcttccatct gcgcttcgat cgacagagga acgtggaccg ccatctggtc
     3781 gccatcgaaa tcggcgttga atgcagcaca caccagcggg tgcagttgga ttgccttgcc
     3841 ttcgatcaag accggctcga acgcctgtat acccagacgg tgcagcgtcg gcgcgcgatt
     3901 cagcatgacc ggatgttctc tgatcacctc ttccaggatg tcccaaacca ccggttcctg
     3961 gatttcgacc agcttcttag ctgccttgag ggttgtcgcc agacccatca gttccagctt
     4021 gttaaagatg aatggcttga acagttccag cgccatcaat ttcggcaagc cgcattgatg
     4081 cagcttcaat tgtggaccca ccacgatcac cgaacggccg gaatagtcga cgcgcttacc
     4141 cagcaagttc tgacggaaac ggccgccctt acccttgatc atttctgcca aggatttcag
     4201 cggacgcttg ttggcgccag tcattgcctt accgcgacgg ccgttatcca gcagcgagtc
     4261 aaccgcttct tgca
//
LOCUS       DASRDH010000275         7278 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1064167,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000275 DASRDH010000000
VERSION     DASRDH010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 7278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7278
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1064167"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="VFA14_06420"
     CDS             <1..187
                     /locus_tag="VFA14_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012435984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipid A hydroxylase LpxO"
                     /protein_id="HZS81042.1"
                     /translation="MMTAASSPNETGDQTGRINKLFRFVWLAGQYRRQFKNWNRHVYQ
                     AVKFGLVAAVVLAIIWL"
     gene            611..2374
                     /locus_tag="VFA14_06425"
     CDS             611..2374
                     /locus_tag="VFA14_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HZS81043.1"
                     /translation="MHAVEQFIQDLAVIMLIAGIVTVVFNRLKQPVVLGYIVAGVIIG
                     AHTPPFNLIQDDKTVHILSELGVIFLLFSLGLEFSLKKLAKVGATAVIGALAEIILMI
                     WVGYEIGIYFGWKTMDAVFLGAMLAVSSTTIIVKALDELGMKQEKFAQIIFGILIVED
                     ILAIGMIALLSGIATSGSVDSGEVFNTVGKLLLFMVVSLVVGILIVPRLLNYVARFKS
                     NEMLLVTVLGILFGFCLLVMKLQYSVALGAFLVGAVMAESRQIHRIERLIESVRDMFS
                     AIFFVAIGLLFDPNVLAQYWLPILVITLAVVFGKLLSCGIGTFLAGHGGRTPMRVGMG
                     LAQIGEFSFIIAALGVSLKVTSDFLYPIVVSVSAVTALLTPYLIKAADPLSARAADLM
                     PRRIAGIFGVYLDWLKRLQPQGEKAEISKIVRRILVQVLINIALVVAIFLAGAFFVDH
                     LGGWLSAWINDVQVRKAVIWGCALALSLPFLIAIYRKMHALAMLLAEVSVKPEFAGVY
                     TASVQRVIAEVVPVMSILGIMLLIFALSTSILPPLNLLVFVLLGEAGLLWLLWSKLVK
                     LHSRLQIALLKTLDEKPEDPH"
     gene            2419..3090
                     /gene="rpiA"
                     /locus_tag="VFA14_06430"
     CDS             2419..3090
                     /gene="rpiA"
                     /locus_tag="VFA14_06430"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HZS81044.1"
                     /translation="MTQDELKQAVARAAIDYVVDGGVIGVGTGSTANFFIDELAKIKD
                     SIEGAAASSETTAARLRGHGIRVFDLDEVETMQVYIDGADEINHQGAMIKGGGAALTR
                     EKIVASAAQKFICIADVSKLVDVLGRFPLPVEVIPMSVSVVARRLSALGCEARLRMKD
                     GKPLVTDNGCHILDAAGLHITAPAGMETAINNIAGVVTVGLFAHQGADICLLSTPEGV
                     RKLEF"
     gene            complement(3111..3470)
                     /locus_tag="VFA14_06435"
     CDS             complement(3111..3470)
                     /locus_tag="VFA14_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS81045.1"
                     /translation="MQEALRYASRLGTKLRAVLGHSFMLIIGQYDALKSTDFSGIAGK
                     SVGMVSVQNNQSKERIINSAIPFAVIDAIEKSTAPHRLRKIVLPDCDHAVSAWLRTNP
                     VRAQWVASEVAEFLSMP"
     gene            complement(3479..3802)
                     /locus_tag="VFA14_06440"
     CDS             complement(3479..3802)
                     /locus_tag="VFA14_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS81046.1"
                     /translation="MISIVVNFPVFTFVSKLQARWQFDETPPLPAHYFSEEFDDYQLE
                     GRVLPAAQQEAELPQLLAIHGARSDYTKLNAILYPLQTLGIASLSLTCLVTTRLRVLD
                     WGTLR"
     gene            complement(3921..4856)
                     /gene="tal"
                     /locus_tag="VFA14_06445"
     CDS             complement(3921..4856)
                     /gene="tal"
                     /locus_tag="VFA14_06445"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013233125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HZS81047.1"
                     /translation="MTQLDQLKQYTTIVADTGDFQAMKAFAPHDATTNPSLILKAVQK
                     DEYQPLLEKIVREHAGQHTVDIIDRLLVAFGLEILKIIPGRVSTETDARLSFDTEGTI
                     AKGRALIDLYEAAGIQRERILIKIASSWEGIRAAEVLEQEGIHCNMTLLFALPQAVAC
                     AEARVQLISPFVGRIYDWYKKSTGQEYSGADDPGVQSVRSIYTYYRKFGYATEVMGAS
                     FRNTAQILELAGCDLLTISPELLQKLAESDAPVARRLSRETAQAAELKKISLDEKAFR
                     LSLNDDAMATEKLAEGIRLFCADAVRLEKIIDELR"
     gene            complement(4942..5796)
                     /locus_tag="VFA14_06450"
     CDS             complement(4942..5796)
                     /locus_tag="VFA14_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016833056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HZS81048.1"
                     /translation="MLLDVIRTHIDSLSRSEKKVAAAILANPQLALQENLTALAGNAG
                     VSEPTVIRFCRAIGHEGWHDFKLKLAQSLALALPGANESLGQDDLAADLVSKICSRSI
                     NTLLDLRNHLDPDAIQRALDVLIRASKIEFYGQGTSGIVANDAQHKFFRSGVPTVAYS
                     DPHIHSIAASLLKPGDAVVAISQRGGNAALLRSVQLARKGGADIIVLGPNGTPLAELA
                     TVLVPIDLSFNIDPYTPISARLAHLVVIDILAVGLALRRGTEFRAKMQDAQKALQEFD
                     MQFDSFFR"
     gene            complement(5819..>7278)
                     /gene="zwf"
                     /locus_tag="VFA14_06455"
     CDS             complement(5819..>7278)
                     /gene="zwf"
                     /locus_tag="VFA14_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HZS81049.1"
                     /translation="SDFDLVLFGGSGDLAMRKLLPALYARDRADDLAPTARIICIGRD
                     NKSREDFLRIVEQNATPHISSVTMDAEIWQRFCMRIQYVSLDARKTASYQHLSAALRD
                     DPAISHVFYLATPPTLFSMICDNLSQTGLATPNARVVLEKPLGHDLESARNINVQVGR
                     YFSESQIFRIDHYLGKEAVQNLLALRFGNVLFEPLWRREWISDVQITIAEELGVGGRF
                     DYYDTSGALRDMLQNHLLQLLCIVAMEPPVSNSADSVRDEKLKVLRSLKRFTPATLAM
                     NVVRGQYRAGHVDGVPVPGYRQEEDANPDSRTETFVAIKAEIDTWRWAGVPFYLRTGK
                     RMADQLAEIVVRFKSVPHSIFANNNDSPNCLVIRLQPDEGLHMNLMAKTPGDGMRLKP
                     VELELDFREKFRMPRMEAYERLLLDVLRGQLTLFMRSDELEAAWEWVEPILEHWEQEE
                     TDPLPYYAGTWGPAAASALIGRDGLQWREEALPEV"
BASE COUNT         1465 a         2004 c         2168 g         1641 t
ORIGIN      
        1 catgatgacc gctgccagct cgcccaatga aaccggagac cagactggcc gcatcaataa
       61 actgttccgt ttcgtctggc tggccggcca ataccgccgc cagttcaaga actggaatcg
      121 ccacgtctat caagccgtca agtttggcct ggtcgccgct gtggtgctgg cgatcatttg
      181 gctgtgagaa cgggaatcca ccgggaattc cggagggatt accacggcac cgtgggcaat
      241 tcggcctgtt tcttacatta cctcttgaaa ggcagagggg gaagtaaaac tcattgggcg
      301 tgttgcgtca agaaaacttg cagatacctt gtcgcctgtg ctaactttgc gcctgccagt
      361 aatggtacgg cgtaaccagt ctgtccgatg tgccgctctg cgctggatgg ctttagaaac
      421 aggctgaaac tcgctgtatt cagcgcatca aggtgtatcc ggctgacgcc gggccgctcc
      481 cttcttcaca atgacgtatt gccgcgcaga cggtaatcta tcgtgtgcct gctccgaaaa
      541 ccggtccggc aatgcgccgc atatcagtca tctgtttcgt caaccatgga ccatagggga
      601 aatataacgc atgcatgctg ttgaacagtt tatccaggat cttgccgtga tcatgctgat
      661 cgccggtatc gtcactgtcg tattcaaccg cctcaagcag ccggtggtgc ttggctatat
      721 cgtggccggc gttattatcg gagcgcacac gccgccattc aatctgatcc aggacgacaa
      781 gacggttcat atcctgtcgg aactgggtgt gatcttcctg ctgttttcac tgggtctgga
      841 gttcagcctg aaaaagctcg ccaaggtcgg cgccacggcc gtcattggcg ctctggcgga
      901 aatcatactg atgatctggg tcggctacga aatcggtatc tacttcggct ggaaaaccat
      961 ggatgcggtt tttctcggcg ccatgctggc ggtatcatcc accaccatca tcgtcaaggc
     1021 gctcgacgaa ctgggcatga aacaggaaaa attcgcccag atcattttcg gcatcctgat
     1081 cgtcgaagac attctcgcca tcggtatgat cgcactgctg tcggggatcg ccaccagcgg
     1141 ttcggtcgac tccggcgaag tgttcaacac cgtaggcaag ctgttgctgt tcatggtggt
     1201 gtcactagtg gtcggcatcc tgatcgtgcc gcgtctgctc aattacgttg cgcgcttcaa
     1261 gagcaacgaa atgctgctgg tcaccgtgct gggcatcctg ttcggcttct gcctgctggt
     1321 gatgaagctg caatatagcg tcgcgcttgg cgccttcctg gtcggcgcgg tgatggccga
     1381 gtcacgccag attcaccgta tcgaacgcct gatcgaatcg gtacgcgaca tgttcagtgc
     1441 gatctttttc gtcgccatcg gcttgctgtt cgacccgaac gtcctggctc aatactggtt
     1501 gccgatcctg gtgatcacgc tggcggtagt gttcggtaaa ctgttgagct gcggcatcgg
     1561 tacgtttctg gctggccatg gtggccgcac accgatgcgc gtgggtatgg gactggcgca
     1621 gatcggcgag ttctccttca tcattgcggc attgggcgtc agcctgaaag tcaccagcga
     1681 ttttctgtat cccatcgtgg tgtcggtatc agcggtgacg gcgttgttga ctccgtatct
     1741 gatcaaggcg gccgacccct tgtcggccag ggccgcggat ctgatgccgc gacggattgc
     1801 tggcattttc ggtgtgtatc tggactggct aaagcgcctg cagccgcagg gagagaaagc
     1861 cgaaatcagc aagatcgttc ggcgcatcct ggtgcaggta ctgatcaata ttgctctggt
     1921 ggtggcgatt ttcctggccg gcgctttttt tgtcgatcat ctcggcggct ggttgtctgc
     1981 ctggatcaac gacgtgcagg tacgcaaagc cgtgatctgg ggttgcgccc tggcattgtc
     2041 gttgccgttc ctgatcgcaa tctaccgcaa gatgcatgca ttggcgatgt tgctggccga
     2101 agtcagcgtc aagccggaat ttgccggcgt atataccgcc agtgtgcagc gtgtgatcgc
     2161 ggaggtcgtt ccggtgatgt cgattctcgg catcatgctg ctgatcttcg cgttgagcac
     2221 cagcattctg ccgccgctca atctgctggt tttcgtgctg ctgggcgagg ccggactgct
     2281 gtggttattg tggagcaaac tggtgaagtt acactcacgc ctgcagattg ccctgctgaa
     2341 aaccctggac gagaagccgg aagatcccca ctgatttcac gcatgcgcac cgaattcgtt
     2401 ttttgacctt ttgccatcat gacgcaagac gaactgaagc aggcagtagc acgcgctgcc
     2461 atcgattacg tggtcgatgg cggagtcatc ggcgtcggta ctggctcgac cgccaatttc
     2521 ttcatcgatg aactggcaaa aatcaaggac agcatcgagg gtgcggcagc ctcgtcggaa
     2581 acgaccgccg cgcgtctgcg cggccatggt atccgggtat tcgacctcga tgaggtcgag
     2641 accatgcagg tctatatcga cggcgccgat gaaatcaacc atcagggcgc gatgatcaag
     2701 ggcggcggcg cagcgctgac gcgcgaaaag atcgtcgcct cggcggcgca gaaatttatc
     2761 tgtatcgccg atgtttccaa gctggtggac gtgctgggca gatttccatt gccggtagaa
     2821 gtgatcccga tgtcggtcag cgtggtggcg cgcagattat cggcactggg gtgcgaagcc
     2881 aggctgcgca tgaaagacgg caaaccgctg gtgaccgaca acggctgcca tattctcgat
     2941 gcggccggtt tgcatatcac ggcgccggcc gggatggaaa cagcgatcaa caacatcgcc
     3001 ggcgttgtga cggtgggcct gttcgcgcat cagggcgcgg atatctgtct gctgagtaca
     3061 ccggaaggcg tcaggaagct ggaattctga gcctctatca tcctagggtg tcacggcata
     3121 gataaaaatt cggcgacctc gctggcaacc cactgcgcac gcacagggtt ggtgcgcagc
     3181 caagcagaga ctgcgtggtc acagtcgggc aatacaatct tgcgcaacct atgcggcgca
     3241 gtgctttttt caatggcgtc aataactgca aaaggaatag cgctattgat aatgcgttcc
     3301 ttcgattgat tgttctgaac actgaccatc ccgaccgatt ttcccgcaat accgctaaaa
     3361 tctgttgatt tcaacgcatc atactgacca atgattagca tgaagctgtg acctaaaacg
     3421 gcacgcagct ttgtgcccaa gcggctagcg tagcgtaaag cttcctgcat attattcctc
     3481 agcgaagtgt cccccagtcc aatacccgaa gccgggttgt gaccagacag gttaaactca
     3541 ggcttgcaat ccccaaggtt tgcaagggat acaggatcgc attgagcttg gtgtagtccg
     3601 accgtgcccc atgaatggcc agcaactgcg gcaattctgc ctcctgctgt gcagctggca
     3661 acacacgacc ttcgagctgg tagtcatcaa actcttcgga aaaatagtgt gccggcagag
     3721 gtggcgtctc atcgaattgc catcgcgcct ggagcttcga gacaaacgtg aatacaggaa
     3781 aattaaccac aatggaaatc aatgcagtgc gttcgtgaat cgaccgtcgt atagaacggc
     3841 gaaagcgtca ggaaactgga attctgattt cgtgaacagg ttctcaggat atcgtcactg
     3901 caaggatggc gcaccaggaa tcagcgcagt tcatcgatga ttttttccag cctgacggcg
     3961 tcggcgcaga acaggcggat gccttccgcg agtttctccg ttgccatggc gtcgtcgttc
     4021 aaggacaggc gaaacgcctt ttcatccagc gagatctttt tcagctcggc tgcttgtgct
     4081 gtttcccggc tcagtctgcg cgcaaccggt gcatcgcttt ccgccagttt ttgcagcagc
     4141 tccgggctga tggtgagcag gtcgcagccg gccagttcga gaatctgcgc agtgttgcga
     4201 aagctcgcgc ccatgacttc ggtggcatag ccgaacttgc ggtagtaggt gtagatgctg
     4261 cgcaccgatt gcacacccgg atcgtcggcg ccgctgtatt cctgtccagt ggattttttg
     4321 taccagtcat agatgcggcc gacgaagggt gagatcagtt gcaccctggc ttcggcgcag
     4381 gcgaccgctt gcggcagcgc gaacagcagc gtcatgttgc aatggatgcc ctcctgctcc
     4441 agcacttcgg cggcgcgaat gccttcccag ctcgaggcga tcttgatcag gatgcgttcg
     4501 cgctgtatgc cagcggcttc atacagatcg atcagcgcgc ggcccttggc aatggtgcct
     4561 tcggtatcga aggacagacg cgcatcggtt tcggtcgaaa cgcgaccagg aatgatcttg
     4621 aggatctcca gaccaaaggc gaccagcagg cggtcgatga tatcgacggt atgttgcccg
     4681 gcatgctcgc gcacgatctt ttccagcaac ggttggtatt catccttttg cacagctttg
     4741 aggatgagcg acggattggt ggtggcgtca tgcggtgcga acgccttcat cgcctggaaa
     4801 tcgccggtgt cggccacgat ggtggtgtat tgcttgagct ggtctaactg cgtcatggca
     4861 atcaatcctg aggtaatgaa atgaaacaaa tgacattcta cgcaagttct cttgcgtcgg
     4921 cggatggtat cgagcgatcg atcagcgaaa gaacgagtcg aactgcatgt cgaattcttg
     4981 cagggctttt tgcgcatcct gcatcttcgc gcggaattcc gtgccgcgtc gcagtgccaa
     5041 cccgaccgcc aggatgtcga tgacgaccag atgcgccagg cgcgcggaga tcggcgtgta
     5101 gggatcgatg ttgaaactca ggtcgatcgg caccagcacc gttgccagtt cggccagcgg
     5161 cgtgccgttc ggtcccagca cgatgatgtc ggcgccgccc ttgcgcgcca gttgcacgct
     5221 gcgcagcaat gccgcattac cgccgcgttg tgaaatcgcc accacggcgt cgccgggttt
     5281 gagcagggat gctgcaatac tgtggatatg cgggtccgag taggcgacgg tcggcacgcc
     5341 ggagcggaaa aacttgtgct gcgcatcatt ggcgacgatg cctgaagtgc cttgaccata
     5401 gaattcgatc ttgctggcgc gtatcaacac atcgagcgcg cgctggatgg cgtcgggatc
     5461 gaggtgattg cgcaaatcca gcaacgtatt gatcgagcgg ctgcaaattt tgctgaccag
     5521 gtcggcggca agatcgtcct gtcccagtga ttcgttggcg ccgggcaagg ccagcgccag
     5581 actttgcgcc agtttcagtt tgaagtcatg ccagccttcg tggccgatgg cgcggcagaa
     5641 gcggatcacg gtcggttccg agacgccggc atttccggcc agcgccgtca ggttttcctg
     5701 aagcgccagc tgaggattgg ccagaatggc ggcagcaacc tttttttccg acctggaaag
     5761 cgagtcgata tgcgtgcgga tgacgtcgag tagcatgaac aggttcctgg gtacgcagct
     5821 acacttccgg caaggcttct tcgcgccatt gcagaccgtc gcggccgatc agcgcgcttg
     5881 ccgccgccgg tccccaggtg ccggcgtaat agggaagcgg atcggtttct tcctgttccc
     5941 aatgctcgag gatgggttcg acccattccc atgctgcttc cagttcgtcg ctgcgcatga
     6001 acagtgtcaa ctggccgcgc agtacgtcga gcagcaggcg ttcgtaggct tccatacgcg
     6061 gcatcctgaa tttttcgcgg aaatccagtt ccagttcgac cggtttcagg cgcatgccgt
     6121 ctcccggtgt tttggccatc agattcatgt gcaggccttc gtcgggttgc aggcggatca
     6181 ccagacagtt cgggctatcg ttgttgttgg cgaagatgga gtgcggcacg ctcttgaagc
     6241 gcaccacgat ttccgccagc tggtcggcca tgcgcttgcc ggtgcgcaga tagaacggca
     6301 cgccggccca gcgccaggta tcgatttccg ccttgatcgc gacgaaggtc tcggtgcggg
     6361 aatcgggatt ggcgtcttct tcctggcgat agcccggcac cgggacgcca tcgacatggc
     6421 cggcgcggta ctggccgcgc acgacgttca ttgccagcgt ggccggggtg aaacgcttga
     6481 gagagcgcag cactttcagc ttttcatcgc gcacggagtc agccgagttg gaaaccggtg
     6541 gttccatggc gacgatacac agcaattgca gcaaatgatt ttgcagcatg tcgcgcaggg
     6601 cgcctgaggt gtcgtaatag tcgaagcgac cgcccacgcc gagttcttcg gcgatggtga
     6661 tctgcacgtc ggaaatccat tcacggcgcc acagcggttc gaacagcacg ttgccaaagc
     6721 gcagcgccag caggttttgc accgcttcct tgccgagata atggtcgatg cggaaaatct
     6781 gcgattcact aaaataacgg ccgacctgga cgttgatatt cctggcgctt tcgagatcgt
     6841 ggcccagcgg tttttccaac accacgcgcg cattcggcgt ggccagtccg gtttgcgaca
     6901 gattgtcgca gatcatcgaa aacagggtcg gtggcgtcgc cagatagaac acatgtgaaa
     6961 tcgccggatc atcgcgcaag gcggcgctga gatgttgata ggaggccgtt tttctggcat
     7021 caagcgaaac atactggatg cgcatgcaga agcgctgcca gatctcggca tccatggtca
     7081 cgctgctgat gtggggtgtg gcattctgct cgacgatgcg cagaaaatct tccctgctct
     7141 tgttgtcgcg accgatgcag atgatgcgcg ccgtcggcgc cagatcatcg gcgcggtcgc
     7201 gcgcatacag cgcaggcaac agcttgcgca tcgccaggtc gccgctgccg ccgaataaaa
     7261 ccaggtcaaa atcggaaa
//
LOCUS       DASRDH010000276         5298 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_697530,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000276 DASRDH010000000
VERSION     DASRDH010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 5298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5298
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_697530"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(181..1659)
                     /locus_tag="VFA14_06460"
     CDS             complement(181..1659)
                     /locus_tag="VFA14_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007879186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HZS81050.1"
                     /translation="MTQTELILLFLIAVGITLQALILLRGRSGVDIDLSLQQLKGELQ
                     RYQTDLAERHVRELCGQVQSGARDTRQELGDNFSRLQQTLAAQMSSVATLQNQQIDSF
                     AQQLVKLNESNAQQLDGMRQTMNLQAQTSREEQAASFKRFGDTLHQTLSTLTESNAQR
                     MAEVRATLEARIKDLQCDNGNRLEEMRKTVDEKLHATLEQRLGESFRLVSDRLDKVHR
                     GLGEMQQLAIGVGDLKRVLTNVKTRGIWGEVQLEMLLEQMLTPEQYARNVETVPHTGE
                     RVEFALRLPGNAEGGAPVWMPIDAKFPKEQYERLADAAERADVEGVAQAGRELERAVR
                     IAARSIAEKYLSPPLTTDFAILFLPTEGLYAEVMRRPGLQDDLQRTHRVSIAGPSTLS
                     ALLNSLQMGFRTLAMEKRSSEVWQVLGAVKTEFGKFGDVLAATRNTLERAARNIEQAQ
                     TRTRQMSRKLKSVEALPDESAQTLLGMAESDVDGDALSEPLS"
     gene            1815..2642
                     /gene="map"
                     /locus_tag="VFA14_06465"
     CDS             1815..2642
                     /gene="map"
                     /locus_tag="VFA14_06465"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013234156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HZS81051.1"
                     /translation="MGTICIKTAEDIAGMRTACKLAAEVLDYIGPFVKAGTTTGELDR
                     LCHAYMTDVQDSIPAPLNYCPPGYTPYPKAICTSLNDVICHGIPGDKIMKNGDVLNID
                     VTIIKNGYHGDTSRMFYIGEPSIMARRLTEITYECMWLGISRVNPGAHLGDIGHIIQQ
                     HAEKAGYSVVREFCGHGIGKAFHEEPQVLHYGRPGTLDKLEAGMIFTVEPMINAGRRE
                     IREMGDGWTIKTRDRSLSAQWEHTVLVTETGFEVLTVSPGMPAPPACVGPARQTAAA"
     gene            2662..5214
                     /locus_tag="VFA14_06470"
     CDS             2662..5214
                     /locus_tag="VFA14_06470"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HZS81052.1"
                     /translation="MSAPALALKRRLQAAHQQTIQAFQADGKTGHLLARLRRHVDAAL
                     TEAWKKSGLPRGTALVAVGGYGRGELFPHSDVDVLILLDRTPDKTMREKLESLVQLLW
                     DIGLEIGHSIRTVEECLAESAADITVQTSLLEARLVSGNTALFRLLQAQCRNAMDPRA
                     FFQAKILEMRQRHIKYEDTPYSLEPNCKESPGGLRDLQVILWVAKAAGLGNSWNRLAR
                     RGLLTATEARQLTRKERVFKDIRVRLHLLTNRREDRLLFDIQTPIAESLGFKSSATRR
                     ASEYLMQRYYRAAKTVTQLNTILLQNIEAQLFPQELVPQKINERFNELNGFLDLAHDR
                     VFEEAPSAMLEVFLILARRQELEDMSARTMRALWHARSRIDDAFRTDPANRALFMQIL
                     QNGHGITHTLRKMNQTGVLGCYLPNFRRIVGQMQHDLFHVYTVDQHILMVVRNLRRFT
                     MSKHAHEYPYCSQLMANFAQPWILYVAALFHDIAKGRGGDHSRLGMADAHRFCKQHGM
                     SEGDTTLVVFLVEHHLTMSHVAQKQDLSDPDVILQFAGTVKDERRLTALYLLTVADIR
                     GTSPKVWNAWKDKLLADLYRMALRVLGGEMPSADRELKNTQQEAIETLRLYGLPADVH
                     ERFWSQLDVAYFLRLDAADIAWQTRSFHDRVDSAIPLVKCRSAPIGEGVQVAVYTPDR
                     PDLFARICSYFDAKNFSILDAKIHTTKRNYALDTFLIDTPACAGNDADIMPLIEHELV
                     QLLQSDNPLPAPARGRLSRLSRAFPIKPSVDLRPDERGQYHLLSISANDRNGLLYAIA
                     SILARHGISLHTAKIMTLGERVEDVFLIDGAALHHPRHQLRLETDLLAALRT"
BASE COUNT         1106 a         1701 c         1454 g         1037 t
ORIGIN      
        1 tcgttctccg gcgcgccgca gatctacacg gattatattt cgattgcagg gaacggacga
       61 tgcgcgtaaa caccccgtga aaaccgtctt tacgcgccag cgcgaatagt cggcgcacta
      121 tatatagttt gagagttgtc gcttacaacc ccatgttgta gccggtggtc tagcgcagtc
      181 tcatgacaac ggctcactca gagcatcgcc gtccacatca gactctgcca ttcccagcag
      241 tgtttgcgcc gactcgtcgg gcagggcttc caccgacttc agcttgcgtg acatctggcg
      301 cgtgcgtgtt tgggcctgtt cgatatttct ggcggcgcgt tccagcgtat tcctggtcgc
      361 cgccagcaca tcgccgaact tgccgaattc ggtcttgacg gcgcccagca cctgccacac
      421 ttccgacgaa cgtttctcca tggccagcgt gcggaaaccc atctgcaaac tgttgagcaa
      481 ggccgacaaa gtcgatggac cggcaatgct gacgcggtgc gtgcgttgca ggtcatcctg
      541 caaaccggga cggcgcatga cttccgcata caagccttcg gtcggcagga acagaatggc
      601 gaaatcggtc gtcaacggcg gcgacagata tttctcggcg atgctcctgg ctgcaatccg
      661 cacagcacgc tccagctcgc ggccggcctg cgctacacct tccacatcgg cgcgctcggc
      721 ggcatcggcc aggcgttcat actgctcctt cggaaacttg gcatcgatcg gcatccagac
      781 cggcgcgcca ccctcggcat tacccggcag cctgagcgca aactcgacac gctcgccggt
      841 atgcggcaca gtctcgacat tcctggcgta ctgctcgggc gtgagcattt gttccagcag
      901 catttccagt tgaacttcgc cccagatacc acgcgtcttg acattggtca gcacacgctt
      961 gaggtcgccc acgccgatgg ccagttgctg catttcaccg aggccccgat ggaccttgtc
     1021 cagacgatcc gacaccagcc tgaaggattc ccccagacgc tgctccagcg tggcgtgcaa
     1081 tttttcatcg acggtcttgc gcatctcttc gagacggttg ccattatcgc attgcagatc
     1141 cttgattctg gcttccagcg tggcgcgtac ttcagccatg cgctgcgcat tcgattcggt
     1201 cagcgtcgac agcgtctgat gcagcgtatc gccgaaacgc ttgaacgacg ccgcctgttc
     1261 ttcacgactg gtctgcgcct gcagattcat ggtctggcgc atgccatcga gttgctgcgc
     1321 attgctttca ttgagcttga ccagctgctg ggcaaagctg tcgatctgct gattctgcaa
     1381 ggtcgcgaca ctgctcatct gtgccgccag cgtctgttgc aagcggctga aattgtcgcc
     1441 aagttcctgg cgcgtgtcgc gcgcgccgga ttgcacctgc ccgcacagtt cccgcacgtg
     1501 acgttcggca agatcggtct gataacgttg cagttcgccc ttgagttgct gcagagacaa
     1561 gtcgatatcg acaccactgc gcccacgcag cagaatcagc gcttgcaggg tgatgccgac
     1621 ggcaatcagg aaaaggagga tcagttcagt ctgggtcatg gataacttgt tttgtctgag
     1681 cttggggcgt gccattctaa gagcctcgac ccttcagatc aatccgatcc agcataccgg
     1741 gagactgaac acgtgaagtc tacctcgttt ataatgactt actcctgttt tcaaaccgca
     1801 tcgcgaccaa ttgcatgggc accatttgca tcaaaaccgc cgaagacatc gcaggcatgc
     1861 gcacagcctg caagcttgcg gcggaagtgc tcgactacat cggccccttc gtcaaggccg
     1921 gcaccaccac cggtgaactg gaccgcctgt gccacgccta tatgaccgac gtgcaggaca
     1981 gcattccggc gccgctgaat tactgcccgc ccggctacac gccgtatccg aaggccatct
     2041 gcacttctct caacgatgtc atctgccacg gcattccggg cgacaaaatc atgaagaacg
     2101 gtgacgtgct gaatatcgat gtgaccatca tcaaaaatgg ttaccacggc gataccagcc
     2161 gtatgttcta catcggcgaa ccgtcgatca tggcgcgccg cctgaccgag atcacctatg
     2221 aatgcatgtg gcttggcatt tccagggtca atcccggcgc ccacctgggc gatatcggcc
     2281 atatcatcca gcagcatgcg gaaaaagccg gttacagcgt ggtgcgcgaa ttttgcggcc
     2341 acggcattgg caaggctttc cacgaagaac cgcaagtcct gcattacgga cgccccggca
     2401 cgctggataa gctggaagca ggcatgatct tcaccgtcga gccgatgatc aacgccggac
     2461 gccgcgagat tcgcgaaatg ggagatggct ggaccatcaa aaccagggat cgcagcctgt
     2521 cggcgcaatg ggaacatacc gtgctggtca ccgaaaccgg cttcgaagtc ctgaccgttt
     2581 caccgggcat gcccgctccg cccgcctgcg tcggacccgc ccgacaaacc gctgccgcct
     2641 gatcattgtt tgcgacgctc catgtcagcg ccggccctcg ctctgaaacg acggttgcag
     2701 gcagcgcatc agcaaaccat ccaggctttt caggctgacg gcaaaaccgg gcatctgctg
     2761 gcgcggctgc gccgtcatgt cgatgccgcc ctgacggaag cctggaaaaa atccggcctg
     2821 ccgcgcggga ccgccctggt agcggtcggc ggttacggtc gtggcgaact gttcccgcac
     2881 tctgacgtcg acgtgctgat cctgctcgat cgaacgcccg acaaaaccat gcgggaaaag
     2941 ctggaatcgc tggtgcagct actctgggat atcgggctgg aaatcggcca tagcatccgc
     3001 accgtcgaag agtgcctggc cgaatcggca gccgatatta ctgtgcagac cagcctgctg
     3061 gaagcgcgtc tggtgagcgg caacacagcg ctgttccgcc tgctgcaagc gcagtgtcgc
     3121 aacgccatgg acccgcgcgc ctttttccag gccaaaatcc tggaaatgcg acagcgccac
     3181 atcaagtacg aagacacgcc ttacagtctg gagccgaact gcaaggaaag tccgggcggc
     3241 ctgcgcgatc tgcaggtgat tctgtgggtc gccaaagcgg cgggcctggg caattcctgg
     3301 aacaggctgg cgcggcgcgg actgctcacc gccaccgagg cgcgccagct cacccggaaa
     3361 gaacgcgtat tcaaagacat ccgcgtccgc ctgcacctgc tcaccaaccg gcgcgaagac
     3421 cgtctgctgt tcgacatcca gacaccgatc gccgagagcc tcggcttcaa gagcagcgcc
     3481 acacgccgcg ccagcgaata cctgatgcaa cggtattacc gggccgccaa gacagttacg
     3541 cagctcaaca cgattttgct gcaaaacatt gaagcgcaac tgttcccaca ggaactggtg
     3601 ccgcaaaaaa tcaacgagcg cttcaatgaa ctcaacggtt tcctcgacct tgcacacgac
     3661 agggtctttg aagaagcacc atccgccatg ttggaagtat ttctgattct ggcccgacgt
     3721 caggagcttg aagacatgtc ggcacggacc atgcgcgcgc tgtggcatgc gcgctccagg
     3781 attgacgatg ccttccgcac cgatcccgcc aatcgcgccc tgttcatgca aattctgcaa
     3841 aacggacacg gcatcacgca tacgctacgc aaaatgaacc agaccggcgt gctgggctgc
     3901 tacctgccaa atttccgccg catcgtcgga cagatgcagc acgacctgtt ccacgtctat
     3961 accgtcgacc agcatatcct gatggtggtg cgcaacctgc gccgcttcac gatgagcaaa
     4021 catgcgcacg aatatccata ctgtagccaa ctgatggcca acttcgcgca gccatggatc
     4081 ctgtacgttg ccgcgctgtt tcatgacatc gccaaaggcc gcggcggcga ccactcccga
     4141 ctgggcatgg ccgatgcgca ccggttctgc aaacagcatg gcatgtcgga aggcgacacc
     4201 acactggtgg tatttctggt cgaacaccac ctgaccatgt cgcacgtggc gcaaaagcag
     4261 gatctgtcag atcccgatgt catcctgcaa ttcgccggca cggtcaaaga cgagcgccgc
     4321 ctgaccgcgc tttacctgct gaccgtcgcc gacatccgcg gcaccagccc gaaggtgtgg
     4381 aacgcctgga aagacaaact gctggcagac ctgtatcgca tggccctgcg cgtactcggc
     4441 ggcgaaatgc catccgctga ccgtgaactg aagaacacgc agcaggaagc catcgagacc
     4501 ctgcgtctct atggcttgcc ggccgacgta cacgaacgat tctggtcgca gctcgacgtc
     4561 gcctatttcc tgcgtctgga cgctgccgac atcgcctggc agacgcgctc attccacgac
     4621 cgggtcgaca gtgccatacc gctggtcaag tgccgctcgg cacccatcgg cgaaggcgta
     4681 caggtcgccg tctacacgcc ggaccggccc gatctgttcg cccgaatctg tagctatttc
     4741 gacgcaaaaa acttcagcat tctcgacgcc aagattcaca cgaccaaacg caactatgca
     4801 ctcgatacct tcctgatcga cacaccggca tgcgccggga atgacgccga catcatgcct
     4861 ctgatcgagc acgaactggt ccagctgctg caatcggaca atccgctgcc ggcgccggcc
     4921 agaggacgcc tgtcgcggct gtcgcgcgcc ttcccgatca agccttcggt ggatttacgc
     4981 cccgacgagc gcggccagta tcatctgctg tcgatatcgg ccaatgaccg caatggcctg
     5041 ctctatgcga tcgccagcat tctggccagg cacgggatca gcctgcacac cgccaagatc
     5101 atgaccctgg gcgagcgcgt ggaagatgtt tttttgatcg acggcgccgc gctccatcat
     5161 ccgcgccatc aactccggct ggaaaccgat ctgcttgccg cgctgcgtac ctaaatgtat
     5221 cgcgcccgat acattgctca gcgtggttgt gccattgctc acgccggcaa accgttgcga
     5281 gtcttcgatc atcagttg
//
LOCUS       DASRDH010000277         3300 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_776109,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000277 DASRDH010000000
VERSION     DASRDH010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 3300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3300
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_776109"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="VFA14_06475"
     CDS             complement(<1..316)
                     /locus_tag="VFA14_06475"
                     /inference="COORDINATES: protein motif:HMM:NF027613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HZS81053.1"
                     /translation="MARTHLFVDSNVFLDFYSYAKDDLGQLKRLIEYLHVDGICLHLP
                     QHVINELERNRETKLKTSAEQFKKENFPTAIPRHMQDYPQAKEYSAAVDTALKKRTAL
                     INQ"
     gene            640..1452
                     /locus_tag="VFA14_06480"
     CDS             640..1452
                     /locus_tag="VFA14_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013697410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HZS81054.1"
                     /translation="MNREELGALLADRLRNRLHASRQQWETTTPINHFYVDDMLPADV
                     AMQIRQAFPDSSAMMLRKSIRELKYVSSQMDEHTPLLEEITFAFQQPEVLALIAEITG
                     LRALQPDENLYAGGISMMGGGHFLNPHIDNSHDKDRERYRVINLLYYVSPEWTLENGG
                     NLELWPNGTKEKPTTVVSQFNRLVVMVTNQHSWHSVSKNVSDQPRCCVSNYYFSDYPV
                     GENEYFHPTSFRGRPEEPIRDVVLQADAALRGLIRTIIPKGVSKTKHVYKKD"
     gene            complement(1449..2939)
                     /locus_tag="VFA14_06485"
     CDS             complement(1449..2939)
                     /locus_tag="VFA14_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006411278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098024 - virus tail, fiber [Evidence
                     IEA];
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - viral entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HZS81055.1"
                     /translation="MSVSLNLKLTSAGQAAVFNAQNNGLKLTLTHIQFGTGKRIPDGT
                     ETALISPSQLSSISSGRKISSTQIRMSALFTGISHYEICEIGIWAGEPEAPESVLFAY
                     YSQATDRIAVMSAGVDFVFSHDMSIDAGIGGSVNIIVDPNGSSMIALMTAHESAADPH
                     SQYATKEGAQILVDDAQSAMRKAMNDMHEEWVKVVSAVASQEQAIAGTDNNTIMTPLR
                     VAQATANKQPALGYTPVQQGTGIGQQPNVIKIGWGSEERLMATVDATDLGNFVFDSQL
                     TSYATMNWVAAKYASVDYVKDRVDSRAPIRVNNGGYGYVINDSGNIITQNWNNHDRID
                     CYVDGKFHGAMAYTWDIEAIRKVLTTKFGSPDGRIKPDWGVGNGFVTLTVDNKGYGIN
                     MHPSDERLKENISPSNKNALKAINQVELFGFNYKENKFLDSSIRHQIGFIAQQLYSVD
                     PTFVIGTPDGDMMMSPNLLSIVAHLVKAVQELSAEVKQLRSASIAD"
     gene            complement(2936..>3300)
                     /locus_tag="VFA14_06490"
     CDS             complement(2936..>3300)
                     /locus_tag="VFA14_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS81056.1"
                     /translation="QFWHGKKSPYPHDLRDEESLDAFLTSRIHITINSFPDPVADSLV
                     MNCLPGVIPARLVPSLRHFVSSSIGKICPAVIASIAQNLKTSGVLGLTFTIGGVSDWV
                     TASTGNSAQIFNSKGTTA"
BASE COUNT          797 a          827 c          851 g          825 t
ORIGIN      
        1 cctgattaat gagggctgtt cgcttcttga gcgcagtatc gaccgctgcg gaatattcct
       61 ttgcttgcgg gtaatcctgc atgtgacggg gaatcgccgt gggaaagttc tctttcttga
      121 attgttcagc tgacgtttta agtttcgtct ccctgttcct ctcaagctca tttatcacgt
      181 gctgagggag atgcagacag atgccatcta catgtaggta ctcaatgagc cgtttcaact
      241 gtccgaggtc gtctttcgcg taggaataga agtcaagaaa gacgtttgaa tcgacgaaaa
      301 gatgagtgcg agccatgtct atttgctatt gctcagtata tgaatacaca caatattgcc
      361 acattcgtaa aatgggcggc actcccgtgc gaaacctctg cagaatgaat ccatatccga
      421 ttagacagat caaatcggtt gggtaacggg aaaaaggcgt attcacgcct ggtcatacgg
      481 cgagacaaag cccggcatgt aaccgggcga aggggtaacc gaaagaaccc ggcacgcagc
      541 cgggttcgaa agggatcact gcgcttggta aataagccga cagtaaggcg tgtgctatcc
      601 tccgacagca aacaacttcg tgattggagc gactacggta tgaacagaga agaactcggg
      661 gccttgcttg cagaccgtct cagaaatcga ttgcacgcat ctcgtcaaca gtgggaaacg
      721 acaaccccca tcaatcattt ctacgtcgat gacatgctgc ctgctgatgt ggcaatgcag
      781 attcggcagg catttccaga ttcatcagca atgatgctcc gaaaaagcat ccgggaactc
      841 aagtacgttt cctctcaaat ggacgagcac acgcctcttc ttgaagagat tacatttgct
      901 ttccagcagc ccgaagtgct tgcgcttatt gccgagatca cgggtcttcg tgcattacaa
      961 ccggatgaga atttgtacgc tggtggtatc tcgatgatgg ggggcggtca cttcctgaac
     1021 ccacacattg ataactcaca cgacaaagat cgcgaacgtt atcgcgttat taatctactg
     1081 tactacgtat cgccagaatg gacgcttgaa aatggtggca acctcgagct atggccgaac
     1141 ggtacgaagg aaaaaccgac gacggttgtg agtcagttca atcgtcttgt tgtcatggtg
     1201 acaaaccagc actcgtggca ttctgtttca aagaacgtga gcgatcagcc ccgttgctgc
     1261 gtgtcgaact attacttctc tgactacccc gttggagaaa atgaatattt tcaccccacc
     1321 tcttttcgtg ggcgcccgga agagccaatc cgtgatgtag tgcttcaagc tgacgcggct
     1381 ctccgtggat tgattcgcac catcatcccg aagggcgtca gcaagacgaa acacgtttac
     1441 aagaaggact agtcagcgat cgatgctgag cgcagttgtt tgacttctgc gctcaattcc
     1501 tgcaccgctt tgactaagtg agcaacgatc gacagcagat tgggcgacat catcatgtcg
     1561 ccgtccgggg tgccgataac aaatgttgga tcgacgctgt atagctgctg cgcgatgaat
     1621 ccgatctggt ggcggattga actgtcgaga aatttgttct ctttatagtt gaaaccaaaa
     1681 agttcgactt gattgatcgc cttcagcgca tttttgttgc tcggagagat gttttccttc
     1741 aagcgttcat cggatgggtg catattgatc ccatagcctt tattatcaac ggtaagtgtc
     1801 acaaacccgt ttccaacgcc ccaatctggt tttatccgac catcgggcga accgaatttt
     1861 gttgtcagca ctttccgtat cgcctcgatg tcccatgtat acgccattgc gccgtggaat
     1921 ttcccatcaa catagcagtc gatacggtcg tgattgttcc agttctgcgt aatgatgttg
     1981 ccggaatcgt tgatcacgta gccatagccg ccgttattga cgcgaatagg cgctcgtgaa
     2041 tcaacgcgat ccttgacata atcgacgctc gcatattttg ctgcaaccca gttcatggtt
     2101 gcgtagctag taagctggct gtcgaagaca aaattgccca gatcggtagc atcgaccgtc
     2161 gccatgagtc gttcttcgct gccccaaccg atcttgatga cattcggttg ctggccaatg
     2221 cctgtgcctt gttgcacggg cgtgtaaccg agggcaggtt gcttattggc ggtagcctga
     2281 gcgacgcgca acggcgtcat gatggtgttg ttgtcggtgc cagcaatggc ttgctcttgc
     2341 gacgcgacag ccgacacaac cttgacccat tcttcatgca tgtcattcat cgctttccgc
     2401 atcgcgctct gggcatcgtc taccaggatc tgcgcaccct ccttggtggc gtactggctg
     2461 tgcggatcgg cagcggattc atgtgctgtc atcagcgcaa tcattgaact cccgttcgga
     2521 tcaacgatga tgttcactga accaccaatg cctgcgtcga tactcatgtc gtgcgaaaac
     2581 acgaagtcca caccagcaga catgacggca atccgatccg tggcctgaga gtagtaggcg
     2641 aagaggacag attctggcgc ttcaggctca ccggcccaga tgccgatttc acaaatttcg
     2701 taatgagaaa tccccgtgaa cagagccgac atacgaatct gggtgctcga tatttttcgt
     2761 ccactggaaa tagacgaaag ttgggacggc gaaatcagcg cggtttccgt cccatcggga
     2821 atacgctttc cggtgccgaa ttgaatatgc gtcaacgtga gtttcaagcc attgttttgg
     2881 gcattaaaaa cggccgcttg acctgctgac gtcagtttca aattaagaga tacactcacg
     2941 ctgtcgtccc tttcgagttg aatatttggg ctgaattgcc cgtgcttgct gtgacccagt
     3001 cggaaacgcc tcctatggtg aaagtcaaac caagaacacc agaagtcttc agattctggg
     3061 cgatactggc aatgacagca gggcaaattt tcccaatgct gctgctgaca aaatgacgca
     3121 ggctgggaac aagtctggcg ggtatcacgc cgggcaggca attcatgacc agactatcgg
     3181 cgacaggatc tggaaagctg ttgatggtga tgtgaatgcg actggtcagg aaggcgtcaa
     3241 ggctttcttc atcgcgcagg tcgtgcgggt atggggattt cttaccatgc caaaactgca
//
LOCUS       DASRDH010000278         5070 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_182206,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000278 DASRDH010000000
VERSION     DASRDH010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 5070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5070
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182206"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..106
                     /locus_tag="VFA14_06495"
     CDS             <1..106
                     /locus_tag="VFA14_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HZS81057.1"
                     /translation="VQLINPIAMEEGLRFAIREGGRTVGAGVVAKIFD"
     gene            192..267
                     /locus_tag="VFA14_06500"
     tRNA            192..267
                     /locus_tag="VFA14_06500"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:225..227,aa:Trp,seq:cca)
     gene            301..681
                     /gene="secE"
                     /locus_tag="VFA14_06505"
     CDS             301..681
                     /gene="secE"
                     /locus_tag="VFA14_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013232176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HZS81058.1"
                     /translation="MSNQSVQTVSNSNDKLKIVLAIVAILAGVVSFFVLSGQSTVVRA
                     LALVAGLLVAVGFAWTSTQGRSFVGFAKESVRETKKVVWPTRKEAMQITAIVFAFVLV
                     MAIFLWGVDKLLEFLLYDVILGWK"
     gene            684..1274
                     /gene="nusG"
                     /locus_tag="VFA14_06510"
     CDS             684..1274
                     /gene="nusG"
                     /locus_tag="VFA14_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006465499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HZS81059.1"
                     /translation="MSDSTQDSALAGAQSASGAGKKRWYVVHAYSGMEKSVRRALTER
                     IARAGMEDKFGQILVPTEEVVDMKNGHKLVTEHRFFPGYILVEMEMTDETWHLVKNTN
                     KVTGFIGGKSNRPTPIPPHEVDKIMQQMQDGIEKPRPKVLYEVGEMVRIKDGPFTDFN
                     GNVEEVNYEKSRVRVSVTIFGRATPVELEFGQVEKA"
     gene            1414..1845
                     /gene="rplK"
                     /locus_tag="VFA14_06515"
     CDS             1414..1845
                     /gene="rplK"
                     /locus_tag="VFA14_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HZS81060.1"
                     /translation="MAKKIIGFIKLQVPAGKANPSPPIGPALGQRGLNIMEFCKAFNA
                     QTQGVEPGLPIPVVITAFADKSFTFVMKTPPATILIKKAAGLQKGSARPHTDKVGKIT
                     RKQVEEIATQKKPDLTAADMDAAVRTIAGSAHSMGITVEGL"
     gene            1845..2540
                     /gene="rplA"
                     /locus_tag="VFA14_06520"
     CDS             1845..2540
                     /gene="rplA"
                     /locus_tag="VFA14_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HZS81061.1"
                     /translation="MAKLTKRAKALQGRVDRTKTYPFDDALVLVKEFATAKFNESIDV
                     SVQLGVDAKKSDQVVRGSVVLPAGTGKAVRVAVFASGEKAEQARMAGADVVGMEDLAE
                     QVKTGNMPFDIVIASPDTMRIVGTLGQILGPRGLMPNPKVGTVTPDVATAVKNAKAGQ
                     VQYRTDKAGIIHATIGRKSFSDAELKSNLVALIDALNKARPATSKGVYLRKVSLSSTM
                     GAGVRVDQATLLA"
     gene            2869..3366
                     /gene="rplJ"
                     /locus_tag="VFA14_06525"
     CDS             2869..3366
                     /gene="rplJ"
                     /locus_tag="VFA14_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006465496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HZS81062.1"
                     /translation="MSLNLNDKKAVVAEVAAKMATAQTVVVAEYRGIQVGHLTQLRAK
                     ARDQGVYLRVLKNTLARRAVDGTAFANLASELTGPLIYSISDDAIAAAKVVSDFAKTN
                     DKLVVKAGNYAGKQLDKTAVVALANIPSREVLLAQLLGVMQAPVAGFARGLAALAAKK
                     EAEAA"
     gene            3469..3852
                     /gene="rplL"
                     /locus_tag="VFA14_06530"
     CDS             3469..3852
                     /gene="rplL"
                     /locus_tag="VFA14_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HZS81063.1"
                     /translation="MAMNKEDILEAVGTLTVLELNDLVKAFEEKFGVSAAAMAVAGPA
                     SGAAGGAAAAAEEQTEFTVVLAEVGANKVGVIKTVREITGLGLKEAKDLVDGAPKPVK
                     EGIAKADAEAAKKKLEEAGAKAELK"
     gene            4063..>5070
                     /gene="rpoB"
                     /locus_tag="VFA14_06535"
     CDS             4063..>5070
                     /gene="rpoB"
                     /locus_tag="VFA14_06535"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006029840.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates; beta subunit
                     is part of the catalytic core which binds with a sigma
                     factor to produce the holoenzyme; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HZS81064.1"
                     /translation="MHYSFTEKKRIRKSFAKRANVHNVPFLLATQIESYQAFLQTDKA
                     ASQRKNEGLQSAFTSIFPIVSHNGFARLEFLSYVLGAPPFDVKECQQRGLTFASPLRA
                     KVRLVILDRESPTKPVVKEMKEQEVYMGELPLMTTTGSFVINGTERVIVSQLHRSPGV
                     FFEHDRGKTHSSGKLLFSARIIPYRGSWLDFEFDPKDILFFRVDRRRKMPVTILLKAI
                     GMTSEQILANFFVFDHFTLHADGAEMEFVPERLRGEVARFDVVDKTGKILVVTDKRIN
                     AKHVRDIEAAGIKQISVPEDYLLGRVLAKGIVDGDTGEVIASANDEITEELLARLREA
                     NI"
BASE COUNT         1187 a         1233 c         1447 g         1203 t
ORIGIN      
        1 tgtgcaactg atcaacccga tcgccatgga agaaggcctg cgcttcgcca ttcgcgaagg
       61 cggtcgtacc gtcggcgcag gtgtcgtcgc caaaattttc gactaagaag cgtcaggaag
      121 ttactgtttg ccgcagtacg aattttaggt ttgttcgtgc tgaggcattt gtgtttcatt
      181 gaggtttttg taggggcgta gctcaattgg cagagcgtcg gtctccaaaa ccgaaggttg
      241 ggggttcgat gccctccgcc cctgccaccg ttcaggtgag tagggtacga aagtaaatca
      301 atgtctaacc agtctgtgca aaccgtcagc aactcaaatg acaagctcaa gattgttttg
      361 gcaatcgtag cgattctcgc aggtgttgtc agtttttttg tattatcggg tcaatctacg
      421 gtagtgcgcg cgctggcctt ggtggccggt ctgctggtgg cagttggttt tgcctggaca
      481 tcgacacaag gtcgcagttt tgtcggtttc gccaaagagt cggtgcgtga aaccaagaag
      541 gtcgtctggc ctacccgcaa ggaggcgatg cagatcacgg cgatcgtttt cgctttcgtg
      601 ctggtcatgg cgatctttct ctggggtgtg gataagttgc ttgaatttct gttgtatgac
      661 gtaattctgg gctggaaata aacatgagtg atagcacaca agacagcgcg ctggctgggg
      721 cgcaaagcgc ttcaggtgcc ggtaagaagc gttggtacgt cgtacatgct tattccggca
      781 tggaaaagag tgtacggcgt gcgctgaccg agcgtattgc gcgtgccggc atggaagaca
      841 agttcggcca gattctggtg ccaactgagg aagtcgtgga tatgaagaat ggtcacaagc
      901 tggtgaccga gcatcgtttc ttcccgggct atatcctggt cgagatggaa atgacggacg
      961 aaacttggca tctggtgaaa aacaccaata aagtaactgg tttcatcggc ggcaagtcga
     1021 accgcccgac gccgattccg ccgcatgaag tcgataaaat catgcagcaa atgcaggatg
     1081 gcatcgaaaa accgcgccca aaagtgctgt acgaagtggg cgaaatggtt cgtatcaagg
     1141 atggcccttt taccgatttc aatggcaatg tagaagaagt gaattacgaa aaatcccgtg
     1201 tacgcgtttc cgtgactatt ttcggccgcg cgacaccagt cgagcttgag tttggtcagg
     1261 tcgaaaaagc ttaggcgggc ggattcaggc aagcgtttgc aggagcgcgg cagaatgtga
     1321 atttcatcga atcctgtaat agcagaggag ctccagtaat gcgttgcaag acaaatgaac
     1381 gggcgttaac actcaatcaa tgaaggagcc gtcatggcaa aaaaaattat cggttttatc
     1441 aagctacaag taccggctgg taaagcaaat ccatctcccc caatcggtcc ggcgctgggt
     1501 cagcgtggtc tgaacattat ggaattctgc aaggcgttca atgcccagac acaaggtgtc
     1561 gagccaggcc tgccgattcc ggtcgtgatc actgcttttg cggacaagtc tttcaccttc
     1621 gtgatgaaga cgcctccagc aactatcctg atcaagaagg ctgcgggtct gcaaaagggc
     1681 tccgccaggc cacatacgga caaggttggc aaaatcaccc gtaaacaggt ggaagaaatc
     1741 gctacccaga agaagccaga tcttactgct gccgatatgg acgcagctgt gcgcaccatc
     1801 gctggttcgg cccattccat gggcatcacg gtggagggac tgtaatggcc aagttgacaa
     1861 aacgcgccaa ggcgctgcaa ggcagggtcg atcgtaccaa gacttatcct ttcgatgacg
     1921 cgctggtgct tgtgaaggag ttcgcaacag cgaagttcaa cgaatcgatc gatgtgtccg
     1981 ttcaactggg cgtcgatgcg aaaaaatcgg accaggtcgt tcgcggttcc gtcgtgttgc
     2041 cggctggtac aggcaaagcc gttcgtgttg ccgtgtttgc ttccggcgaa aaggcagaac
     2101 aggccaggat ggctggcgcg gacgtcgttg gtatggaaga tctggcggaa caggtcaaga
     2161 ctggcaacat gcctttcgac atcgtcatcg cttcgccaga caccatgcgt atcgtcggca
     2221 ccctgggtca gatcctgggg ccgcgtggcc tgatgcctaa tccgaaggtc ggcactgtga
     2281 cgcctgacgt cgctaccgct gtcaagaacg ccaaggctgg tcaggtgcaa taccgtaccg
     2341 acaaggccgg catcattcat gccaccatcg gtcgcaaatc tttctcagat gcagagttga
     2401 agtccaatct ggttgcgctg atcgatgcac tgaacaaggc caggccggcc accagcaagg
     2461 gggtttacct gcgcaaggta tcgctgtcgt ccaccatggg cgcaggcgtt cgtgtggatc
     2521 aagctacatt gctggcttaa gtaaagaatt aagtcctcat ttcttcacgg aaatgaggca
     2581 tatctttggg ctgaagcatt ttgcgattgt gaagtgcttc gggcaatcaa agaccgctgg
     2641 gcagcgtgca gcaggcaggc acaaagttaa aacttcaaaa acttctgttt ttgatgaccc
     2701 agcgcagatg gtgaacccga acaggttttg cagtctcacg cggcatctca ggtggcatgg
     2761 aatgctgtgg aactcttaag ctcaggtcgg atgccgtgtt cgaaccgatg taaggcaagc
     2821 aggtttttcg cctgtgtcgt cgccaagcat tatttttgga ggttgatctt gagtctcaat
     2881 ctgaatgaca aaaaggccgt cgtcgccgaa gtcgctgcca aaatggcaac tgcacagact
     2941 gtcgtcgtgg ccgaataccg tggtatccag gttggtcact tgacgcagct gcgcgctaaa
     3001 gcgcgtgacc agggcgtgta tctgcgtgta ttgaaaaata cactcgcgcg tcgcgctgtc
     3061 gatggcaccg catttgccaa cctcgcttct gagttgaccg gtccgttgat ttattcgatt
     3121 tcggatgatg ccattgccgc agcgaaagtc gtttccgact tcgctaagac aaatgacaaa
     3181 ctggtcgtca aggcaggtaa ctatgctggc aagcagctgg ataaaacagc tgtcgtcgca
     3241 ttggcaaata tcccgagccg tgaagtgctg ctggcccagt tgctgggtgt gatgcaggct
     3301 ccggtcgctg gttttgcacg tggtctggct gctctggcag ccaaaaagga agctgaagcc
     3361 gcctgatcga tcatgtttcg cagtgatgcg gatttgcatg ttgcgtgctt tgagtttcag
     3421 cgtcagcacc aattagatgt tattaccgaa ataaattagg agtttcaaat ggcaatgaat
     3481 aaagaggata tcctggaagc cgtaggcacc ctgaccgtgc tggaattgaa tgacctggtg
     3541 aaggcatttg aagagaaatt tggcgtttcc gctgcagcga tggctgttgc cggtcctgca
     3601 agcggcgctg ctggcggtgc agcagctgct gctgaagagc aaaccgagtt tactgttgtt
     3661 ctggcggaag tcggcgcgaa caaggttggt gtcatcaaaa cggttcgtga aatcacaggc
     3721 ctgggcctga aagaagccaa ggatctggtc gatggcgcgc cgaagcctgt caaagaaggt
     3781 atcgccaagg cagatgctga agcagccaag aaaaaactgg aagaagctgg tgcgaaggca
     3841 gaactcaagt aatgatttga gctggaatat tgccggttcg ccggcgcatc gttttaccgg
     3901 agtcaaagtg cagagtcctt tgaaagaaga tctcggcttc gactcctttg tcgtttcaga
     3961 attttgtatg tgaatttgag ttcgattcgc atcgatatcg gattcaagtc cgtcaatgcg
     4021 gagcctgaac tctccattct tcctgtcact cacggagtgt ccatgcacta ctcatttact
     4081 gagaagaagc gtattcgtaa atcttttgcg aaacgcgcta acgtccacaa cgttccgttt
     4141 ctgttagcga ctcagatcga gtcatatcag gcattcttgc aaacggacaa agcagcatcc
     4201 cagcgcaaaa atgaaggtct gcaatcggct tttacgtcga tttttcctat cgtttcgcac
     4261 aatggttttg cgcgtctcga atttttgtcc tatgtgttgg gggcgccgcc gttcgacgtc
     4321 aaggaatgcc agcagcgcgg cctgactttc gcgtcgccat tgcgcgccaa agtacgtctg
     4381 gtgatcctgg accgggagtc gcctaccaag ccggttgtca aggaaatgaa ggagcaggaa
     4441 gtctacatgg gcgaattgcc gctcatgacg accaccggct cgtttgtcat caacggtact
     4501 gagcgtgtca tcgtatcgca gctgcatcgt tcgcctggcg tgtttttcga gcatgaccgt
     4561 ggcaagacac actcgtccgg taaattgctg ttctccgcac gtatcattcc ttaccgcggt
     4621 tcgtggctcg attttgaatt cgatccgaag gatattctgt tctttcgtgt cgaccgccgc
     4681 cgcaagatgc ctgtgacgat tttgctcaag gcaatcggca tgacatccga gcagattctg
     4741 gcgaacttct tcgtgttcga tcatttcact ctgcatgccg acggcgccga aatggaattt
     4801 gtccccgaac gtctacgcgg cgaagtggcg cgtttcgatg tcgtcgacaa gacaggcaag
     4861 attctggtcg tgacagacaa gcgtatcaac gccaagcatg tgcgcgacat cgaagccgcc
     4921 ggtatcaagc agatttccgt gccggaagat tacctgctgg gtcgcgttct ggcgaagggc
     4981 atcgtcgatg gcgacaccgg tgaagtcatc gcgtcggcca acgacgagat aaccgaagaa
     5041 ctactggcgc gcctgcgcga agccaatatc
//
LOCUS       DASRDH010000279         2311 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1282345,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000279 DASRDH010000000
VERSION     DASRDH010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1282345"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="VFA14_06540"
     CDS             <1..573
                     /locus_tag="VFA14_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS81065.1"
                     /translation="FSETQIFNAVLDRLAEESVLTTTDEKAVSHWLNRNYPLQRDVLL
                     QRHTWNFAMARRQLAADTNKPPFEWSFQYLLPEDCLRVCPVTADGRRNSPPIPYVVEG
                     MRILTNKSAPLNVRYISRMIDTNLFTPVFIDLLAQILAANFCYWVTGKANFSKQLMDI
                     SSLSFLEAARIDSLEGLPEEPYDDEIIMVR"
     gene            577..>2311
                     /locus_tag="VFA14_06545"
     CDS             577..>2311
                     /locus_tag="VFA14_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS81066.1"
                     /translation="MPGPIYPLQPVFARGELSPRLFSRVDIDHYKMGLAECVNWLVMK
                     QGGLRRRPGTEWISWGKYPHVKCRLVRFVFSTQQAYVLEWGSYYVRFFANGGIVAKNS
                     RFNITFSGGYLVDWPAHGLVFNDPVVFSSSGYLPPEIAPGTTYYVSAVTTDNFTISII
                     AGGAGMTFSNAGTGTHNVVSPVELPTPYTLDEVFQLQFAQSADVLYIAHPNHQQQMLS
                     RFSGSTFTFLPYSGYDGPYLPENTTSTVMVASGSALGPDITITADSVTGINGNAGFQP
                     SDVGRWLSLKYSNKWYSFLITSYVAVGEVVTFSIGLIDDKGAQQPLPSEATATAGWRL
                     GAWSEVTGWPGCVAFYQQRLIWARSDTQPQTIWMSRAGVLDNFSVTSPLQEDDALTIT
                     ILAGEVNAIAWIAEGADLLIGTNGAMRTLGPADSSKNFGPTNFMQKRQSTFGTLNIQP
                     VQVGEVAIYPSFYGLSLREFLFSFQVNGYVSPELTILSEHMLRSGIKAMAYAQDKDGI
                     IWNAMGNGELVGITYDRDQQIVACQRHRIGGEVKNSGIVDPNNPSDPDTHYGIVESVT
                     TIPGADRSEVWL"
BASE COUNT          483 a          635 c          638 g          555 t
ORIGIN      
        1 ttctccgaga cgcagatttt caacgctgtg ctcgaccggc tggcggagga atcggtgctg
       61 accaccacag atgagaaggc ggtcagtcac tggctcaatc gcaactaccc actccagcgc
      121 gatgtgctgt tgcagcggca cacttggaat tttgcgatgg cgcggcggca gcttgccgcc
      181 gacaccaaca agccgccgtt cgagtggtcc tttcagtatc tgttgccgga ggattgcctg
      241 cgagtttgtc ctgtcacggc ggacggacgg cgcaattcgc cgccgatccc gtacgtagtg
      301 gagggtatgc gtattttgac caacaaatct gcgccgctta acgtgcgcta tatctcacgc
      361 atgatcgaca ccaatttgtt cacgccggtc tttattgatc tgctggcgca gatactcgcc
      421 gccaattttt gttactgggt caccggcaag gccaactttt ccaagcagct tatggatatt
      481 tccagcctgt cttttctgga agctgcccgt atcgacagcc tcgaagggtt gccggaagag
      541 ccttacgacg acgaaatcat catggtccgc taggccatgc cgggaccgat atatccactt
      601 caaccggtct ttgcgcgggg cgagctgtct ccccgccttt tttcgcgcgt cgacatcgac
      661 cactacaaga tggggctggc cgagtgcgtc aactggctgg tcatgaagca gggcggattg
      721 cgccgccgtc ccggcaccga atggatcagt tggggcaagt atccgcatgt gaaatgccgt
      781 ctggtgcgct ttgtcttttc cacccagcaa gcctatgtgc tggaatgggg atcatattat
      841 gtacgttttt ttgccaatgg cggaattgtt gccaagaact ctcgttttaa cataactttt
      901 tcaggcggct atcttgtgga ctggccagcc cacggtctcg tcttcaatga cccggtggtg
      961 ttctcgtcca gcgggtattt gccgccggaa atagcgccgg ggactacgta ttatgtgagc
     1021 gctgtcacca ccgacaattt tacaatctcg attattgctg gtggcgcagg aatgacattc
     1081 agtaatgccg ggaccggcac acacaatgtt gtttcaccgg ttgagctgcc aacgccatat
     1141 acgcttgatg aagtttttca actgcaattt gcccaatcgg ctgacgttct ctacatcgcg
     1201 cacccaaatc accaacagca gatgttgtca cgcttttccg ggtctacttt tactttcctt
     1261 ccgtactcag gttacgacgg gccatacttg ccagagaaca ccacatcaac ggtgatggtg
     1321 gcgtccggca gcgcccttgg tcctgacatt acgattactg ccgattccgt gaccggcatt
     1381 aacggcaatg ccgggtttca gccatccgat gtggggcgct ggcttagcct caaatattcc
     1441 aacaaatggt acagctttct gattaccagc tatgttgccg tgggagaggt tgtcaccttt
     1501 tcaatcgggc tgattgacga caagggcgca caacagccgc ttccgtccga agccacggca
     1561 accgctggct ggcgtcttgg cgcatggtcg gaagtaaccg gatggccggg atgtgtcgcg
     1621 ttctaccagc aacgtctgat ctgggcgcgg agcgacacgc agccgcagac gatctggatg
     1681 agcagggcgg gcgttctgga caacttttca gtcacttctc cgttgcagga agacgacgcc
     1741 cttacgatca ccattcttgc aggcgaggta aatgccattg catggattgc cgaaggggcc
     1801 gatctgttga ttggcaccaa cggggcaatg cggacgcttg gtccggcaga ttccagcaag
     1861 aattttggtc ccaccaactt tatgcagaaa agacaatcga ctttcggaac attgaacatc
     1921 cagccggtac aggttggaga ggtggcgatt tatcccagtt tttacgggct gtccttgcgg
     1981 gaatttctgt tttcgtttca ggtcaacggt tatgtctcgc ctgagctgac catcctgtca
     2041 gagcacatgc tgcgatccgg catcaaggca atggcttacg cacaggacaa ggacggaatt
     2101 atctggaacg cgatgggcaa tggcgagctg gtcgggatca cctatgaccg cgaccagcag
     2161 atcgtggcgt gtcagcgcca tcgtatcggc ggtgaagtca aaaactcggg cattgtcgac
     2221 ccgaacaatc caagcgatcc ggatacccac tatggcattg tcgagagcgt caccaccatt
     2281 cccggtgcgg atcgctccga ggtctggctt a
//
LOCUS       DASRDH010000280         3751 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1205780,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000280 DASRDH010000000
VERSION     DASRDH010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 3751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3751
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1205780"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..873)
                     /locus_tag="VFA14_06550"
     CDS             complement(<1..873)
                     /locus_tag="VFA14_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008333675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZS81067.1"
                     /translation="MAQYVFTMNRVGKIVPPKRQILKNISLSFFPGAKIGVLGLNGSG
                     KSTLLKIMAGIDREIEGEAIPMPNLNIGYLPQEPQLDASKTVRQEVESALGEVFEAQA
                     RLDAVYAAYAEENADFDALATEQARLEAVIASADGNNLNQQLEMAADALRLPPWDARI
                     GVLSGGEKRRVALCKLLLSKPDMLLLDEPTNHLDAESVDWLEQFLQRFPGTVVAITHD
                     RYFLDNAAEWILELDRGHGIPWKGNYSSWLEQKKARLKQEEASESARQKTIAKELEWV
                     RQNPKGRQAKSKARL"
     gene            complement(917..1789)
                     /gene="rarD"
                     /locus_tag="VFA14_06555"
     CDS             complement(917..1789)
                     /gene="rarD"
                     /locus_tag="VFA14_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HZS81068.1"
                     /translation="MFYAISSFLLWGLFPFYFRLLHDVSALDIVANRVFWSLLFLVAM
                     LTWKRQWAWCGEVARKPRVLLVFLLSALLLSGNWLSYVWAVNSDRIVDASFGYFMSPL
                     ISVLLGCLIFKEKLRPLQWMAVLMALGAVSWLTWMSGALPWIGLTIAVTFSIYGLMRK
                     IAHLGALEGLALETMLLSPLVLLVLIVSTLHGSNGFLQASLTSQILLALSGPVTAIPL
                     LLFAHAARRIPLSLLGLLQYITPTMQLLTGVFIYDEPFGGARMTAFIVIWTALALYSL
                     EGLWRLWTARIRPA"
     gene            complement(1887..3113)
                     /locus_tag="VFA14_06560"
     CDS             complement(1887..3113)
                     /locus_tag="VFA14_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008120023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HZS81069.1"
                     /translation="MHTDSSPLQGKRFPGWLILMAALTAMSALSIDMYLPSFSFIARD
                     LGVGSNTVELTLSTFLIGLALGQMFYGPISDRFGRKPPLYFGICLYVVSALGCALAPN
                     IETLLALRFFQGLGGSVGMVIPRAVVRDKMGAEGSARAFSMLMLVFGLAPILAPFIGG
                     LLLTLSTWRAIFATLVVFGAICLISTHHLMEETRDRAYVMPLHLGRVLRQYGDLLRQR
                     QFMAYVLCGGLIQAGMFAYISGSPFVLIELHGIEPQHFGFVFGANAIGLIVCSQINAR
                     LLIRRSADRVLGNIIWIPAALATAVAILIACGFESLPLLLIGFFGFLSCQGFVGPNTA
                     AIALAQQGRQAGTAAALMGTLQFGIGMLSSVLMGWWHDGTALPLTVVMAVCGVTALLL
                     YRYVARHTMRAAPCQT"
     gene            complement(3246..>3751)
                     /locus_tag="VFA14_06565"
     CDS             complement(3246..>3751)
                     /locus_tag="VFA14_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017772226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HZS81070.1"
                     /translation="LIDQALVLFGAPHSLSATLRQQRDHAQVPDYVHLQLHYADKEVI
                     LHAGALTALPPPRFTVHGTRGSYVIAGLDVQEDQLKAGLRPGDAGFGKNPDGLLREVD
                     GGSEVQAAYATQDGDYASFYRALADTLLDGAPFPVLADDAVDGMTLIELAMRSDAEGR
                     RLPFVRT"
BASE COUNT          859 a         1139 c         1111 g          642 t
ORIGIN      
        1 cagacgcgcc ttgctcttgg cctggcggcc cttcggattt tgccgtaccc attcgagttc
       61 tttggcgatg gttttttgcc gcgccgattc actggcttct tcctgcttca gacgcgcttt
      121 cttctgctcc agccaggacg aataattgcc cttccatgga atgccgtggc cgcgatccag
      181 ttccagaatc cactcggcgg cattgtcgag aaaatagcgg tcatgggtaa tcgccacgac
      241 ggtgccgggg aaacgttgca ggaactgctc cagccagtcc accgattcgg cgtccagatg
      301 gttggtcggc tcgtcgagca acagcatgtc gggcttggac agcagcagtt tgcacagcgc
      361 cacgcggcgc ttttcaccgc ccgacagcac gccgattctg gcgtcccacg gcggcaggcg
      421 caaggcgtcg gcggccattt ccagctgctg gtttagattg ttgccatcag cgctggcaat
      481 gaccgcttct agacgcgcct gctcggtggc gagcgcatcg aagtcggcgt tttcttcggc
      541 ataagcggcg tagacggcat cgagcctggc ttgcgcttcg aacacttcgc cgagcgcgct
      601 ttcgacttcc tgacgcacgg tcttgctggc atcgagctgt ggttcctgcg gcaggtagcc
      661 gatgttcagg ttcggcatcg gaatcgcttc gccttcgatt tccctgtcga tgccggccat
      721 gatcttgagc agagtcgatt tgcccgaacc gttcagaccg agtacgccga ttttggctcc
      781 cgggaagaaa gagagcgaaa tgtttttcag aatctgccgc ttcggcggca cgatcttgcc
      841 gacgcggttc atggtgaata cgtattgagc cataactgga tgtcaggtaa tgagaaaaga
      901 aaagagtggc ggcggctcag gccgggcgga ttctggcagt ccacagccgc cacaatcctt
      961 ccagggagta tagcgccaac gccgtccaga tcacgataaa cgctgtcatg cgcgctccgc
     1021 cgaacggttc gtcgtagatg aaaacaccgg tcaacagttg catcgtgggg gtaatgtatt
     1081 gcagcaatcc cagcaatgac aagggaatac ggcgcgctgc gtgggcgaac agcaataacg
     1141 gaatcgccgt caccggtccc gacagcgcca gcagaatctg cgacgtcagc gacgcctgta
     1201 aaaagccgtt gctgccgtgc aacgtcgaca cgatcaacac cagcagcacc agcggcgaca
     1261 gcagcatggt ttccagcgcc agcccctcca gcgcaccgag gtgtgcaatc ttgcgcatca
     1321 ggccgtagat gctgaaggtg acggcaatcg tcagaccgat ccaaggcaaa gcgccgctca
     1381 tccaggtcag ccacgacacg gcgcccagcg ccatgagtac tgccatccat tgcaaaggac
     1441 gcagcttttc tttgaagatg aggcagccga gcagcacgct gatcagcgga ctcatgaaat
     1501 aaccgaaact ggcatcgacg atgcggtcgc tattgaccgc ccacacatac gacaaccagt
     1561 tgcctgacaa caacaaggcg ctcaacaaaa aaaccagcag cacgcgcggc ttacgcgcga
     1621 cttccccgca ccaggcccat tgacgcttcc aggtcagcat ggcaaccaga aaaagcagcg
     1681 accagaaaac ccggttggcc acgatgtcca gggcggagac gtcgtgcagc aacctgaagt
     1741 agaacgggaa caagccccag aggagaaaac tgcttatcgc gtaaaacatg gcgacctgga
     1801 aaatggaaac gtgaaatggt acacagaatc cgtcttttcc ggctagaaat gactaaaatg
     1861 actatccagc agccgcgttg gcgggatcaa gtctgacacg gcgcggcacg catggtgtga
     1921 cgcgcgacat agcgatacag cagcagcgcg gtcacgccgc acaccgccat cacgacagtc
     1981 agcggcagcg cagtgccgtc atgccaccac cccatgagca cgctggaaag catgccgatg
     2041 ccgaattgca gcgtacccat cagcgccgcg gcggtgccgg cctgcctacc ctgctgcgcc
     2101 agtgcgattg ctgcggtgtt gggaccgacg aaaccctggc aggacaggaa gccgaaaaat
     2161 ccgatcagca gcagcggcag gctttcaaaa ccgcaggcga tcagtatggc caccgccgtg
     2221 gccagcgcag ccggaatcca gatgatgttg ccgagtacgc ggtcggcgct gcgcctgatc
     2281 agcaggcgcg cattgatttg tgaacaaacg atcaggccga tggcgttggc gccgaagacg
     2341 aagccgaaat gctgcggctc gatgccgtgc agttcgatca gcacgaacgg actgccggag
     2401 atataggcga acatcccggc ctggatcaga ccgccgcaca gaacataagc catgaactgg
     2461 cgctgccgca gcaaatcgcc atactgacgc agcacgcggc ccagatgcag cggcatcaca
     2521 tacgcgcgat cgcgggtttc ttccatcagg tgatgcgtgc tgatcaggca gatggcgccg
     2581 aataccacca gcgtcgcaaa aatcgcgcgc caggtcgaca acgtcagcag caggccgccg
     2641 atgaatggcg ccaggatcgg cgccaggcca aacaccagca tcagcatcga gaaggcgcgg
     2701 gcagaacctt ccgcccccat cttgtcgcgc accaccgcgc gcggaatcac catgccgacg
     2761 ctgccgccca gaccctggaa gaagcgcagc gccagcagcg tttcgatgtt cggcgccagc
     2821 gcacatccca gcgccgacac tacatacaga cagatgccga agtacagcgg cggcttgcgg
     2881 ccgaaacggt cactgatcgg accgtagaac atttgcccga gcgccagtcc gatcagaaaa
     2941 gtcgacaacg tcagctcgac cgtattgctg cccacgccga ggtctctggc gataaaggaa
     3001 aaactgggca gatacatgtc gatcgacaag gcgctcatag ccgtcagcgc cgccatcaga
     3061 atcaaccagc cagggaagcg tttgccctgc aagggcgaag aatcggtatg catgaaaaga
     3121 aatgataaaa accgcctgat acagaagacg gtcgatacga gaaggggatg ctttgcgccg
     3181 gaaaggtagg gtgtcagtat accaccgtcc gggcgcggcc cggtggtggt atcgtcaaca
     3241 cgttttcagg tccggacaaa cggcaggcgc cggccttcgg cgtcgctacg catggcaagt
     3301 tcgatgaggg tcatgccatc gaccgcatcg tcggccagca ccgggaaagg cgcgccatcg
     3361 agcagcgtgt cggccagcgc gcgataaaag ctggcatagt cgccgtcctg ggtggcatat
     3421 gcggcctgta cctcactgcc gccatcgact tcgcgcagca aaccgtccgg attcttaccg
     3481 aaaccggcat cgcccggacg cagaccggct ttgagctgat cttcctggac atcgagtccg
     3541 gcgatgacat aactgccgcg cgtgccatgc acggtgaaac gcggcggcgg caaggcggtc
     3601 agcgcgccag catgcaggat gacttccttg tcggcataat gcagttgcag gtggacatag
     3661 tccggcacct gggcatggtc gcgttgctgt ctcagggtgg ccgacaagga atgtggcgcg
     3721 ccgaacagca ccagcgcctg atcgatcaga t
//
LOCUS       DASRDH010000281         2141 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1314946,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000281 DASRDH010000000
VERSION     DASRDH010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1314946"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="VFA14_06570"
     CDS             <1..344
                     /locus_tag="VFA14_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZS81071.1"
                     /translation="SGIATHRVLLGVYVLAGFLYGIAALLSVARMGVGDPQAGQTDNL
                     DSITAVVLGGTSLFGGRGTIVGTLIGVLIVGVFRNGLTLMGVPSVFQILVTGLLVILA
                     VAADQFTHKKA"
     gene            349..1143
                     /locus_tag="VFA14_06575"
     CDS             349..1143
                     /locus_tag="VFA14_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZS81072.1"
                     /translation="MTANTSISIFQAHDIVKRYGHVTALDGVDFDLRAGEILAVVGDN
                     GAGKSSLIKALSGALIPDSGEMLLDGRKVYFKSPIDARRHGIETVYQDLAVAPAMTIA
                     ENLFLGREVLKLGIFGKCLRIIDKKQMLSEAVRHMQNLKIGIRSMSQPVSTLSGGQRQ
                     GVAVARSTAFARHVVILDEPTAALGVKESNMVLELIRRVRDRGLSVILISHNMPHVFE
                     IADRIHIQRLGKRVAVVDPKKISMADTVAVMTGAVNPQDLPKDAYA"
     gene            1340..>2141
                     /locus_tag="VFA14_06580"
     CDS             1340..>2141
                     /locus_tag="VFA14_06580"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HZS81073.1"
                     /translation="MIATIGEALVDLIEQQDGRFQACLGGSVCNFTLGVARQGISTAY
                     LNPLSKDKFGEKFSALLSGNGVVLKANGGSASPTALAVVSLDDQGSPTYAFHRQGVAD
                     RDISAERLIASLPESLELLHTGGLALVPGDLEKILTVVREVGRRKALVSIDANLRPIA
                     VTACDAYVEGVQQAIAQAHILKVSDEDLAILGFGDATPTMLAATLFHESTLQLIAVTH
                     GVRGATLLTRAYQMTLPAPNDLKVADTVGAGDCFHAGLISYLARAGKLA"
BASE COUNT          458 a          504 c          666 g          513 t
ORIGIN      
        1 tgagcggcat tgccacccat cgggtgttat tgggcgtata tgtactggcc gggtttttat
       61 atggcattgc cgcgctgcta tccgtggcgc ggatgggagt cggcgatccg caggccgggc
      121 agacggataa cctcgacagc atcacggcgg tagtgttggg cggcaccagt ctgttcggcg
      181 gtcgtggcac gattgtcggt acattgatcg gcgtattgat tgtgggcgtg tttcgcaatg
      241 gactaacctt gatgggcgtg ccgtcggtat tccagattct ggtgaccggc cttctagtga
      301 ttctggctgt cgcggcagat cagtttacgc acaagaaagc ttgaaaaaat gactgcgaat
      361 acttcaattt cgatttttca ggcgcatgac atcgttaaac gatatggcca tgtgacggcg
      421 ctcgatggtg tcgatttcga tttgcgcgcc ggcgaaattc tggcggtcgt cggcgacaac
      481 ggcgccggta aatcctcctt gatcaaggca ttgtcaggcg cattgattcc agactccggc
      541 gagatgttgc tggatggtcg caaggtgtat ttcaaatctc cgatcgatgc gcgtcggcat
      601 ggtatcgaga cggtttatca ggatttggcg gtggcaccgg cgatgacgat tgcagaaaat
      661 ctttttcttg ggcgggaggt attgaagcta ggcatattcg ggaaatgtct gcgcatcatc
      721 gacaagaagc aaatgctgtc cgaggccgtc cggcatatgc agaacctgaa aatcggtatt
      781 cgttccatga gccaaccggt atcgaccttg tccggcggtc agcgtcaggg cgtggccgtc
      841 gcgcgcagta cggcgtttgc caggcatgtg gtgatcctgg acgagccgac tgcggcgctc
      901 ggcgtgaagg agagtaatat ggtcctggaa ttgatccgca gggtacgtga tcgcggtctg
      961 tcggtgattc tgatcagcca caatatgccg catgtattcg agattgcgga tcgcattcat
     1021 attcagcgcc ttggaaaacg tgtcgccgtt gtcgatccga agaaaatcag tatggccgat
     1081 acggttgccg tgatgacggg agccgtgaat ccgcaggact tgccgaagga tgcgtatgct
     1141 taaaggtatt gacccgctgc tgtcgccgaa tttgctcagg gtcttgtgca aatgggacat
     1201 ggggatgaag ccgtgctggc aaacgccaat tagttctgaa aacgaatcag atcgggatgc
     1261 gtcaattcaa cgaacgcgtg gtgttacaga ccatacgttt tcacggcggt cgaaagtttg
     1321 aacgcatttt gaagacatca tgattgcaac aataggggaa gcactggttg acctaattga
     1381 acagcaggac ggccggtttc aggcttgcct tggcggttcg gtatgcaatt tcacgctggg
     1441 agtagcgcgt cagggtatat cgacagcgta tctcaatccc ttatcgaagg ataaattcgg
     1501 agaaaaattt tctgccctgt tatcaggcaa tggtgttgtt ttaaaggcaa atggcggcag
     1561 tgcgtcgcca accgcgctag ccgtggtgag cctcgatgat cagggttcgc cgacatacgc
     1621 atttcaccgg caaggggtgg ccgatcgtga tatcagtgct gagaggctga tcgccagttt
     1681 gcctgagtcg ctggaattgc tgcacaccgg cggcctggcg ctggttccgg gtgatcttga
     1741 gaaaatcctg acagtggtac gtgaagtggg aagacggaag gcgcttgtgt caattgacgc
     1801 caacttgagg ccgatagcgg tcacggcatg cgatgcctat gtcgaaggtg tgcaacaggc
     1861 aattgcacag gcgcatatcc tgaaggtcag cgacgaagac ctggcgattc ttggcttcgg
     1921 cgatgcgacg ccgacaatgc ttgcggctac tctgtttcat gaatcgacgt tgcagctgat
     1981 cgctgtcacg cacggtgtgc gaggagcaac attgctgaca cgcgcatacc agatgacgct
     2041 gccagcgcca aacgatctga aggtggctga tacggttggt gcgggcgact gctttcatgc
     2101 cggactgatt tcttatctgg cgcgcgccgg caaacttgcc g
//
LOCUS       DASRDH010000282         4376 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_510646,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000282 DASRDH010000000
VERSION     DASRDH010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 4376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4376
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510646"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="VFA14_06585"
     CDS             <1..264
                     /locus_tag="VFA14_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007882225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA helicase"
                     /protein_id="HZS81074.1"
                     /translation="DMLHDIERFIKRDIPVEIIPGFEPDPKAKPQPVPLRSNGRAQQK
                     PGRAGQSRPPSAGTRQGGSARAQGQGPAARPTSTTHRSGGRSR"
     gene            complement(229..858)
                     /locus_tag="VFA14_06590"
     CDS             complement(229..858)
                     /locus_tag="VFA14_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HZS81075.1"
                     /translation="MQLDQAFSDEEFDELDDFLLSDRCAEDAMTMDTLHGFLTALVIG
                     PQEIPMAEWLPRVWGSMDEPRFGSPREAERIIGLITHMMNEIVITFKVAPEEYEPLFC
                     EYRYEGESLLDGEAWAWGFWEGVQLRTVEWDPVFHSGIADLMRPIYLLGADELEEEEG
                     RLVDDPVKRHRLAIEVESAIPHLYRHWLPHRKPAARTGSPAAATGAMRR"
     gene            986..1969
                     /locus_tag="VFA14_06595"
     CDS             986..1969
                     /locus_tag="VFA14_06595"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HZS81076.1"
                     /translation="MNAEAPFFDLAPDRVLDAIESLGTRCDGRLLQLNSFENRVYQVW
                     LEDGNQRVVKFYRPGRWSDEAIFEEHAFSLELADQEIPVVPPLTFDGRTLHHHEGHRF
                     ALFAKQGGRTPELDREETLEWLGRFLSRIHAVGKNFHYQTRPRLDIQSLGRDPRHFLL
                     AGAWLPLELQSAWSSVADQALDCVSHAYERAGDVQTLRLHGDCHPGNLLWTDDGPHFV
                     DFDDSRTGPAIQDLWMLLSGDAADMRTQFRALMKGYETFLDFDDRELHLIEALRTLRL
                     IHYSAWIAGRWSDPAFPAAFPWFGSVRYWQERILELREQVALMQEPSFTAA"
     gene            2031..3425
                     /locus_tag="VFA14_06600"
     CDS             2031..3425
                     /locus_tag="VFA14_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3696 domain-containing protein"
                     /protein_id="HZS81077.1"
                     /translation="MLTSLHIKNFKAWKDTGPIRLAPLTVIFGANSAGKSSLGHLLLA
                     LKQTVLSADRKRPLHLGDENALIDLGTFAECLHNHDLQKVLDFELGWKTQGKDLEARD
                     SLSKKKFAGDELKLSVILSADPKGQPRVDKLAYQLLRKSAKQLAVDYSRDGKGKLEIT
                     SNEYKFVRNTGRGWPLDEPDKFYRISDQSRARFQNAEFLSDFALNTEAVLNSVYYLGP
                     LREHPKRIYSWSGETPESVGQKGEFAVAAILAASAQERMLNRGPRKHLTRFDEFIAQW
                     LKDLGIIESFAVKPVAKGRKEYEVVIKTHATASEVKITDVGFGVSQVLPALVQSFYCP
                     PNSTIWMEQPEIHLHPQVQAELADVFISATQAHENGKKRHVQLIVESHSEYFLNRLQR
                     RVAEGIVAPEDVAVYFCRRAGAATELEPLRLNMFGEIENWPENFFGDEMADIAGRTLA
                     AMQHKKELAKEAGK"
     gene            3527..3955
                     /locus_tag="VFA14_06605"
     CDS             3527..3955
                     /locus_tag="VFA14_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS81078.1"
                     /translation="MEKAGVTVIDDDFRILREYQHKTSLNPPKGVGDVFLKWLLRQAG
                     NPARVEQVSLTELAANEFAEFPDIALQPAFDPPDRKFVAVAHAHPNKPSVWQAADCKW
                     LDWRPALQDNGIRVEFLCLDDACRFYHNKFPNKPLPALPD"
     gene            3959..>4376
                     /locus_tag="VFA14_06610"
     CDS             3959..>4376
                     /locus_tag="VFA14_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005800654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase family protein"
                     /protein_id="HZS81079.1"
                     /translation="MTDFFRFPHTPHLAWLGEGSPRDDKVMSLIEVAALLAGEVAVEE
                     KLDGANIGLSLAPDGGLRVQNRGQYLAKPHAGQFIHLPAWLAQHGNALCSTLTPNIIL
                     FGEWCAARHSLDYAALPDWFLLFDVYERSAGRFWNSS"
BASE COUNT         1032 a         1161 c         1202 g          981 t
ORIGIN      
        1 gacatgctgc acgatatcga acgcttcatc aaacgtgaca tcccggtcga aatcatcccc
       61 ggcttcgaac ccgaccccaa ggccaaaccg cagccggtgc cattgcgcag caacggtcgt
      121 gcgcagcaga aaccgggcag agccgggcag agccgcccgc catcagccgg aacacgccag
      181 ggcggttccg ccagggcgca aggccaaggc cctgccgcgc gccctacttc aacgacgcat
      241 cgctccggtg gccgcagcag gtgacccggt tcgcgcagct ggtttgcgat gcggcaacca
      301 atgacggtag agatgcggaa tcgccgattc cacttcgatc gccagtcgat gccttttgac
      361 cgggtcatcg accagccggc cctcctcttc ctccagctcg tctgcgccga gcagatagat
      421 cgggcgcatc aggtcggcga tgccggaatg gaaaaccgga tcccactcca cggtccgtaa
      481 ctggacgccc tcccagaaac cccaggccca ggcttcgcca tccagcagac tttctccttc
      541 atatctgtac tcgcagaaca gcggttcgta ttcttcgggc gcaactttga atgtgatgac
      601 gatctcattc atcatgtgtg tgatcaggcc gatgatgcgc tcggcttccc ggggcgatcc
      661 gaaccggggt tcgtccatgc tgccccaaac gcgcggcagc cactcggcca tcggaatttc
      721 ctgcgggccg atcaccaacg cggtgagaaa accatgcagc gtgtccatcg tcatggcgtc
      781 ttcggcgcag cggtcggata acaggaaatc gtcgagttcg tcgaactctt catcggagaa
      841 cgcctggtcc agttgcatga aataccatcc gtatggtgga taaaaccaca gtgtaattcc
      901 gatcgcctgt cgaggcgagg atcaccactg ctgggcacgt acaatagatg ctgacaagaa
      961 gtgatgttat cgggaacaac caaccatgaa tgctgaagct cctttttttg atttggcccc
     1021 cgatagggta ttggacgcga ttgaaagcct cgggacacgc tgtgatggac gattgctgca
     1081 actgaatagt tttgagaatc gtgtctacca ggtttggctc gaagatggta accagcgtgt
     1141 tgtgaagttt tatcggccag gacgctggag tgatgaggct atcttcgaag aacacgcatt
     1201 cagtctcgaa cttgctgatc aggaaattcc agtggttccg ccgctgacgt tcgacggacg
     1261 cacactgcat catcatgaag gccaccggtt tgccctcttt gccaaacagg gagggcgaac
     1321 accagagctt gatcgggaag aaacgcttga gtggttagga cgtttcttga gtcgtattca
     1381 tgctgtcggg aaaaatttcc attaccagac aaggcccaga ctcgatatcc aaagcctggg
     1441 gcgtgaccca cgtcactttc tccttgcggg tgcatggttg cccctggaat tgcagtcagc
     1501 gtggtccagc gtggcagatc aggcgctgga ttgcgtgagt cacgcctatg aacgtgcagg
     1561 tgatgtgcaa acattgcggt tacatggcga ttgccatcca ggaaacctgt tgtggacaga
     1621 tgatggtcct cattttgtcg atttcgacga cagtcgaacc ggtccggcaa ttcaagatct
     1681 ctggatgctg ctttcgggcg atgctgcgga tatgcgcacg caattcaggg cattgatgaa
     1741 aggctatgaa accttcctcg atttcgatga tcgtgaactg catctcatcg aagcgcttcg
     1801 cacgctacga ttgatccatt acagcgcctg gatcgccggg cgatggagcg atcctgcctt
     1861 ccccgccgca tttccatggt ttggcagtgt tcgttactgg caggagcgca tccttgagct
     1921 gcgcgagcaa gtcgcactga tgcaggaacc ttcgttcacc gcggcttgat aataagcttt
     1981 cctcaaaatt tggatgcatg ggataaattg aaaataggag aattgcactg atgctgactt
     2041 ctttgcacat caaaaatttc aaagcctgga aggataccgg acccatacga ctggcgccgt
     2101 tgacggtgat ctttggtgcc aatagcgcgg gtaagagtag cttgggacat ttattgctgg
     2161 cgctcaaaca aactgtctta tcggcagatc gaaaacggcc actccattta ggtgacgaaa
     2221 atgccctaat tgatttgggc acttttgcgg aatgtttgca taaccacgat ttgcagaaag
     2281 tcctagactt tgaactggga tggaaaactc aaggtaaaga tctggaggcg cgtgattcgc
     2341 tcagcaagaa aaaatttgcg ggtgatgaat taaagctttc agtcatacta tctgctgatc
     2401 ctaaaggcca gcctagggtg gataagctgg cgtaccaact cttgcgcaaa agcgcaaagc
     2461 agcttgctgt tgattacagc agggacggta aaggtaagct cgaaattacg tcaaacgaat
     2521 ataaatttgt gcgcaatacg ggtagaggct ggccgctgga tgagccagac aagttttacc
     2581 gtatttctga ccaaagccgt gcccgctttc aaaatgcgga atttttgtct gattttgcgc
     2641 tgaatacgga ggcggtacta aatagcgttt actaccttgg gccactacgc gagcacccca
     2701 aacgcatcta ttcatggtca ggcgaaacgc cagaaagcgt tggacaaaag ggcgagtttg
     2761 ccgttgccgc cattctggct gccagcgcgc aagaacgaat gctgaatcgt ggcccgagaa
     2821 agcatctgac ccgctttgat gaattcatcg cgcaatggct caaggatttg ggaatcatcg
     2881 aaagctttgc ggtgaagcct gtagccaaag gccggaaaga atatgaggtg gtaattaaaa
     2941 cccacgccac agccagcgaa gtcaaaatta ccgatgttgg ctttggcgtg tcacaggttt
     3001 taccggccct ggttcagtct ttctattgcc cgccgaattc gaccatctgg atggagcagc
     3061 cagaaatcca tttgcatcct caggtgcaag ccgagcttgc tgatgtgttt atttcggcca
     3121 cacaggccca cgaaaatggc aaaaaacgcc atgttcaact gattgttgaa agccattccg
     3181 aatatttcct caatcgcctg caacgccgcg tcgccgaagg catcgtggca ccggaggatg
     3241 tggccgtgta cttctgtcgt cgcgccggtg ccgccaccga gctggaaccg ctgcgcctga
     3301 acatgtttgg cgaaatcgag aactggccag aaaatttctt cggcgacgag atggctgata
     3361 ttgctggccg caccttggcg gcgatgcaac acaagaagga actcgccaag gaagcaggca
     3421 aatgatgagg gcggtcattg ataccaatgt tctgctggtg gccaacgggc agcatgctga
     3481 cgtatcgacg gagtgtgtaa ccgaatgtgt gcaacgccta caggccatgg aaaaggctgg
     3541 cgtaacggtc attgacgatg attttcgtat tctgcgtgaa tatcaacaca aaaccagcct
     3601 caatccaccc aaaggggttg gcgatgtgtt cctgaaatgg ttgctacgcc aagcaggaaa
     3661 ccctgcgcga gtggagcaag tcagcctgac cgagttggcc gcaaatgaat tcgccgaatt
     3721 cccggatata gccttgcaac ccgcgtttga tccaccggat cgcaaattcg tggcagtcgc
     3781 tcacgctcat ccaaataagc cgtctgtatg gcaagcagca gactgcaaat ggttggactg
     3841 gagaccagcc ttgcaggaca acgggattcg tgtcgaattt ctctgcctgg acgacgcttg
     3901 caggttttat cacaataagt ttcccaacaa accgctgcct gcattgccgg actgagccat
     3961 gaccgatttc ttccgatttc cccacacacc tcaccttgcc tggttgggtg aaggctcgcc
     4021 acgcgatgac aaggtgatgt cgctcattga ggtagcggcg ttgctggcag gtgaagtggc
     4081 ggttgaggaa aagctcgatg gcgcaaatat cggcctgtct ctcgccccgg atggcggtct
     4141 ccgcgtacag aaccgggggc aatatctggc gaaaccccac gccggtcaat tcatccactt
     4201 gcctgcgtgg ctagcgcagc atggtaacgc actatgctct acactgacac ccaacatcat
     4261 tctgttcggt gaatggtgtg ccgcgcgcca ttcgctggat tatgcagcgc tgccagactg
     4321 gttcctgctc tttgatgtgt atgagcgcag cgcaggccgt ttctggaaca gttcgc
//
LOCUS       DASRDH010000283         2757 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_493874,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000283 DASRDH010000000
VERSION     DASRDH010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493874"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="VFA14_06615"
     CDS             complement(<1..289)
                     /locus_tag="VFA14_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013233940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HZS81080.1"
                     /translation="MVFNIPIFLTWLRVALIPLVVGVFYIPEIGLSRFEQGLASTIIF
                     IVAAVTDWFDGFFARRWNQTSAFGAFLDPVADKLMVTGALLVLVHLDRVHPV"
     gene            complement(308..2164)
                     /gene="uvrC"
                     /locus_tag="VFA14_06620"
     CDS             complement(308..2164)
                     /gene="uvrC"
                     /locus_tag="VFA14_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HZS81081.1"
                     /translation="MSDILPFDQRGIILETVARLPHLPGVYRYFDAGDNALYVGKARD
                     LKKRVSSYFQKTLASPRIAMMVERIARLETTVTRSEAEALLLENNLIKTLRPRFNILF
                     RDDKSYPYLKISGHVFPRMAYYRGAVDKKNQYFGPFPNAAAVKESIQILQKVFLLRTC
                     EDTVYANRTRPCLLHQIHRCSGPCVNLVTQENYALDVESAAAFLRGRQTEVLDVLEGK
                     MQTYAGALKFEQAAAVRNQITSLSSVLHQQTMETGSDADIDIIAVIVQGGRACVNLAM
                     VRGGRHLGDRACFPAHMDSAIADGEASLETAVMKAFLAQHYIDKFIPANLIVSTEFDD
                     PALMLALMEQCGHRITLTLQPQGQRRQWLEMARKGAEIALTRLLSEQGAQQVRTRALI
                     EALGREIEDIGSLRVECFDISHMQGEATQASCVVFHHHAMQHGEYRRYNINGITPGDD
                     YAAMRQVLMRRYEKAAHGDGVMPGIVLIDGGKGQVETARQVFAELGLDIGAIVGVAKG
                     KERKVGLETLVFADGRAPQELGKESAALMLIAQIRDEAHRFAITGMRARRDKVRQTSR
                     LEEIEGIGAKRRQKLLARFGGLRGVADASIDELASVEGISHTLAEEIYRQLH"
     gene            complement(2186..>2757)
                     /gene="nagZ"
                     /locus_tag="VFA14_06625"
     CDS             complement(2186..>2757)
                     /gene="nagZ"
                     /locus_tag="VFA14_06625"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HZS81082.1"
                     /translation="LAKSLNHGLALAGMANCGKHFPGHGYVAADSHVDLPVDERSLKD
                     IIGDDAAPYDWLGMSLSAVMPAHVVYPKVDRRPAGFSGKWMKILRQDIGFQGAIFSDD
                     LSMHGASVAGGVLDGARAALQAGCDMVLICNAPDKAEQLLEGLETGGSPASAARIAAL
                     APRGGAPDWEALQKDERFLTAKKLVQTLR"
BASE COUNT          595 a          859 c          742 g          561 t
ORIGIN      
        1 tgaccggatg cacacgatcc agatgcacca gcaccagcaa ggcgccggtg accatcagct
       61 tgtccgcgac cggatcaaga aaggctccga atgccgacgt ctggttccag cgccgggcaa
      121 aaaagccatc gaaccagtca gtaacagcag ccacgatgaa gatgatcgtc gaagccagcc
      181 cttgctcgaa acgcgacaaa ccgatttcag gaatataaaa cacaccgact accagcggaa
      241 tcaaggccac acgcaaccag gtaagaaaaa tagggatatt gaataccatg cgtttcctta
      301 tcttccatca atgcaactgg cgataaatct cttcagccag cgtatgcgaa ataccctcta
      361 ccgatgccag ttcatcgata ctggcatccg ccacgccgcg caggccgccg aagcgcgcca
      421 gtagcttttg acggcgtttg gcgccaatgc cttcgatctc ttccagacgc gatgtctgcc
      481 gcactttatc gcgtctggcg cgcatgccgg tgatagcaaa gcggtgcgct tcatcacgga
      541 tttgcgcaat cagcatcaac gcggcggact ccttacccag ttcctgcggc gcacgaccat
      601 cggcgaagac caaggtttcc agaccgacct tgcgctcctt ccctttggcg acaccaacga
      661 tcgcgccgat gtccagcccc agctcggcaa atacctggcg cgccgtttca acctgcccct
      721 tgccgccgtc gatgagtacg atgccgggca tgacgccatc accgtgagcg gctttttcat
      781 aacggcgcat cagcacctgg cgcatcgcgg catagtcatc accgggggta atgccattga
      841 tattgtagcg acggtattcg ccatgctgca tggcatgatg gtgaaatacc acgcaggaag
      901 cctgcgtcgc ctcgccttgc atatgactga tgtcgaaaca ttccacgcgc aggctgccga
      961 tgtcttcgat ttctctcccc agcgcctcga tcagcgcgcg cgtgcgcacc tgctgcgcac
     1021 cttgttcaga caacagtcgc gtcaacgcaa tctccgcgcc cttgcgcgcc atctccagcc
     1081 attgacgacg ctggccttgc ggctgcaacg tcagcgtaat gcggtgtccg cattgctcca
     1141 tgagcgccaa catcagcgcg ggatcatcga attcggtact gacgatcagg ttagcgggaa
     1201 tgaatttatc gatgtaatgc tgcgccagga aggccttcat gaccgctgtc tcgagtgatg
     1261 cctcgccatc ggcaatggcg ctatccatat gcgccgggaa acaggcgcga tcacccagat
     1321 ggcgaccgcc gcgcaccatg gccagattga cgcaggcgcg accgccctgc acgatcacgg
     1381 caatgatgtc gatatcggca tcactaccgg tttccatggt ctgctgatgc agcacgctcg
     1441 acagcgatgt gatctggttg cgcactgccg cagcttgctc gaatttgagc gcaccggcat
     1501 aggtctgcat tttcccttcc agaacatcga ggacttcagt ctgacggccg cgcaggaatg
     1561 ctgcggcgct ctcgacgtcg agcgcatagt tttcctgcgt taccagattc acgcacggtc
     1621 cgctgcagcg atgaatctgg tgcagcagac aaggccgggt gcgattggca tagaccgtat
     1681 cttcgcaggt acgcaacaaa aataccttct gcaaaatctg gatcgactcc ttgaccgccg
     1741 cggcgttcgg aaatgggccg aagtactgat ttttcttgtc aactgcgccg cgataatacg
     1801 ccatgcgtgg aaaaacatgg ccgctgatct tcagataggg ataggatttg tcgtcgcgaa
     1861 acaggatatt gaagcgcggc cgcaaagtct tgatcagatt gttttccagc agcagcgcct
     1921 ctgcctcgct acgcgtaact gtcgtttcca ggcgcgcgat gcgttcgacc atcatggcga
     1981 tgcgcgggct ggccagtgtt ttctgaaaat aactggagac acgctttttc agatcgcgcg
     2041 ccttaccgac gtagagcgcg ttgtccccgg cgtcaaaata gcgatacacg cccggcagat
     2101 gcggcagcct ggcgactgtc tccaggatga tcccgcgctg gtcgaaaggc aagatatcgg
     2161 acatgtgaat ctatcagctc atgtgtcagc gcagcgtctg taccagtttt ttcgccgtca
     2221 ggaagcgctc gtctttttgt agcgcttccc agtccggcgc gccgcccctc ggcgccaggg
     2281 cggcaatgcg cgcagccgat gcgggtgagc ccccggtctc cagtccctcc agcaactgtt
     2341 cagccttgtc cggtgcatta cagatcagca ccatgtcgca accggcctgc aacgcggccc
     2401 tggcgccgtc gagtacaccg ccggcaacac tggcgccgtg catactgagg tcgtcactga
     2461 agatcgcacc ctgaaaaccg atgtcctggc gcagaatttt catccatttt cctgaaaatc
     2521 ccgccgggcg cctgtccacc ttgggataaa cgacgtgcgc cggcatcacg gcgctcagac
     2581 tcataccgag ccagtcgtaa ggcgcggcat cgtcgccgat gatgtctttc agagaacgtt
     2641 catcgaccgg caaatcaaca tgcgaatccg ccgcgacata accgtgaccg gggaaatgct
     2701 tgccgcagtt ggccatacca gccagcgcca gaccgtgatt gagacttttc gccagca
//
LOCUS       DASRDH010000284         5900 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_860973,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000284 DASRDH010000000
VERSION     DASRDH010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 5900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5900
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_860973"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1218
                     /locus_tag="VFA14_06630"
     CDS             <1..1218
                     /locus_tag="VFA14_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008112174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HZS81083.1"
                     /translation="RQIQWYSARPGHMQRTSERASRYLFHVVQELEKRGMPTELALLP
                     FIESAFDPQALSTAKAAGMWQFIPSTGLHYKLKQNMFTDERRDVLASTDAALTYLQKL
                     HGMFNDWQLALAAYNWGEGAVQRAVNRNLAAGLPIDFDTLSAQMPGETRNYVPKLQAV
                     KNIIAMPGDYGIDLPSVDNQPYFVSIGKTRNIDVKIAAQLAELPLDEFNALNPQFNRP
                     VIIGGKDARILLPESHAEKFKANLSRFTQTLSSWTMHTVSGTRERIETIASRFRTTPD
                     VIRSVNQIPPKMLLEAGSSILVPKTGAAMETEITPAMASSARLLIVPDVPDFKHIYVK
                     AGKRDTLHSVASRYHVSVAQIKQWNSLHRDTLTHGQRLRLQLSPAARMRQGVQAPARP
                     VRKASVVARNPRT"
     gene            complement(1227..2813)
                     /locus_tag="VFA14_06635"
     CDS             complement(1227..2813)
                     /locus_tag="VFA14_06635"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HZS81084.1"
                     /translation="MSLLDIRNLSVRFGAATVVDGVSFTIAPGEKFALVGESGSGKTV
                     SALSVLRLNQDAHYSGQILFNGEDILQKSEAAMRGIRGMAAAMIFQEPMTALNPLFTI
                     GNQIAEVLMLHEGLSAKTAAQRTVALLEKTGIPEPARLTLAYPHQLSGGQRQRAMIAM
                     ALACHPRLLIADEPTTALDVTIQVQILELLNQLQRDENMAVLLISHDLNLVRHFADRV
                     GVMEQGRLAETAATAALFAAPQQAYTRTLLASQPQRQINDVAADAPVLLSGDHLRCTF
                     SIKRGWLHKTSFTAVDDVSLRLRKGETLGIVGESGSGKSTLGMALLRLSMATVQGDIH
                     FDGKPISAMTAGAIRPLRARMQVVFQDPFASLSPRRTIVQIVGEGLALHQPHLSAAQQ
                     RAEVVAALREVGLSEDMLQRYPHEFSGGQRQRIAIARAVVLRPELLLLDEPTSALDVT
                     VQQQVLQLLTQLQQKYGLAYLFISHDLAVIRAMAHQVLVMQDGKVVERGNADVVLDHP
                     TQTYTQCLLAAATYARRESM"
     gene            complement(2810..3853)
                     /locus_tag="VFA14_06640"
     CDS             complement(2810..3853)
                     /locus_tag="VFA14_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZS81085.1"
                     /translation="MNAALLSPPVSPGRRIWLRFRQSRRGYWSLIIFLALFAFSLVAE
                     LVSNDRPLIASYHGQILFPVAHDYSEKTFGGDFDSPADYLDPFIQDQLHRDGNWAIYP
                     LNRYRFDTLNYFAREPNPAPPSSENWLGTDDRGRDVFARLLYGFRISILFGLALTVTG
                     VALGLLAGAAQGYFAGRTDLFMQRILEIWGAMPELYLLIIFSSIFEPGIGLLLLLLSL
                     FGWMGLSDYVRADFLRNRHLEYVQAARALGLSNMQIIWRHVLPNSLTPVVTFLPFRMS
                     AAILALTSLDFLGLGVPPSMPSLGELLAQGKNNLDAWWIALSTFIALSVTLLLLTNIG
                     DALRNALDVRRKA"
     gene            complement(3850..4908)
                     /locus_tag="VFA14_06645"
     CDS             complement(3850..4908)
                     /locus_tag="VFA14_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006464346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HZS81086.1"
                     /translation="MNIWSYILKRILLMLPTLFGVVAITFAVIQFVPGGPVEQALIEM
                     KGRGGAGEASGGGASPYRARQGIDAGRIDEIKALYGFDKPPVERFWHMLKGFASFDLG
                     KSFYHHGDVWQLILSKMPVSITLGLWTFFLTYLVSVPLGIAKAVRAGSHFDALTSAVV
                     LVGYSIPGFVLGVFLLVMFGGGSFVQWFPLRGLTSDDWAALSLFGKLKDYLWHITLPV
                     TASVTGAFAVMTMLTKNSFLEEIRKQYVLTARAKGLSENKVLYKHVLRNALIPLVTGF
                     PAAFIGAFFAGSLLIETLFSLDGLGLLSYESVIRRDYPVVMGSLYLFTLIGLVVKLLG
                     DLCYVWADPRIQFESLAG"
     gene            complement(4905..>5900)
                     /locus_tag="VFA14_06650"
     CDS             complement(4905..>5900)
                     /locus_tag="VFA14_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016833261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZS81087.1"
                     /translation="FDVVVENSAKNWVRNYSGPKFTSGEIVKRELKHANGAGMQGFVM
                     NLRRDRFRDPRVRRALGLALDYEWMNRQLFYGQYKRIYSFFNNSDLAATGVPAGAELK
                     LLESIRAAGIRIDPAVFGPAPVPPSTDPPASLRANLLQARELLRQAGWEYRDGALRNA
                     GSEVFAFEILDDQSALSRVISIYVRNLQKLGIQVTQRSADYALVAKRMDEFDYDMTSI
                     RFPDVSSPGNELFDMFGSRAADEKGSNNVWGLKDPAIDKLVEALVAADTRADLVAAAR
                     GLDRVLLHKYIVVPQWYASTHRVAYRNRFGIPAIVPLYYQADPYVISTWWEKSPK"
BASE COUNT         1292 a         1809 c         1725 g         1074 t
ORIGIN      
        1 agacagatcc agtggtatag cgcgcgcccc ggtcatatgc agcgcacctc cgagcgcgct
       61 tcgcgttacc tgttccacgt cgtgcaggaa ctggaaaaac gcggcatgcc gaccgaactg
      121 gcgctgctgc ccttcatcga atccgccttc gatccgcaag ccttatcgac tgccaaagcg
      181 gccggcatgt ggcagttcat tccctccact ggcctccact acaagctgaa gcagaatatg
      241 ttcaccgatg agcgtcgtga cgtgctggcc tcgaccgatg cagcgctgac ctatctgcag
      301 aaactgcatg gcatgttcaa cgactggcaa ctggcgctgg cggcctataa ctggggcgaa
      361 ggtgcggtgc agcgtgccgt caaccgcaat ctggccgccg gcctgccgat cgacttcgat
      421 acactgtcgg cgcaaatgcc cggcgagacg cgcaactacg tgcccaagct gcaagccgtc
      481 aagaacatca tcgccatgcc gggcgactac ggcatcgatt tgcccagcgt cgataaccag
      541 ccttatttcg tgagcatcgg taaaacccgc aatatcgacg tgaaaattgc cgcgcaactg
      601 gccgaattgc cgctcgacga attcaatgcc ctgaatccgc aattcaaccg gccggtcatc
      661 attggcggca aagacgccag gattctgctg ccggaaagcc atgccgagaa attcaaggcc
      721 aatctctcca ggttcacgca aaccctgtcg tcatggacca tgcataccgt ttccggcacg
      781 cgcgaacgca tcgaaaccat cgccagcagg ttccgcacca cgccggatgt gattcgcagc
      841 gtcaaccaga ttccaccgaa aatgctcctc gaggccggct ccagcattct ggtgccgaag
      901 accggggcgg cgatggaaac cgagatcact cccgccatgg ccagcagcgc ccgcttgctc
      961 atcgtgccgg acgtgcccga cttcaagcac atctacgtga aggctggcaa acgcgacaca
     1021 ctgcattccg tcgccagtcg ttaccacgtc agtgtggccc agatcaagca atggaacagc
     1081 ctgcatcgcg acacgctaac gcatggtcag cgcctgcgat tgcagctgtc gccggcggcg
     1141 cgcatgcgcc agggcgtcca ggcgccggca agaccagtgc gcaaagcctc tgtcgtcgcg
     1201 aggaaccccc gcacgtagca gcgccgctac attgactctc tgcgggcata cgtcgctgcc
     1261 gccagcaggc actgcgtata ggtctgtgtc ggatggtcca gcaccacgtc ggcgttaccg
     1321 cgctcgacca ccttgccgtc ctgcatgacc agtacctgat gtgccatggc gcggatgacg
     1381 gcgaggtcgt gactgatgaa cagataagcc aggccatatt tctgctgcaa ttgcgtcagc
     1441 agttgcagca cctgctgctg cacggtgacg tcgagcgccg aggtcggttc gtccagcagc
     1501 agtaactcgg gtctgagcac cacggcgcgg gcgatggcga tgcgctggcg ctggccgccg
     1561 gagaattcat gcggataacg ttgcagcatg tcttcggaca gcccgacttc gcgcaaggca
     1621 gccaccactt cggcacgctg ctgcgccgcg ctcagatgcg gttgatgcag cgccagacct
     1681 tcgccgacga tctgtacgat cgtccggcgt ggcgacaatg aggcaaatgg atcttggaac
     1741 accacctgca tacgcgcgcg caacggccgt atcgcgccgg ccgtcatggc gctgatcggc
     1801 ttgccgtcga aatggatgtc gccctgaacc gtcgccatgg acaaacgcag caaggccatg
     1861 cccagcgttg attttccgga accggattcg ccgacgatgc cgagggtctc gcccttgcgc
     1921 aagcgcagac tgacgtcatc gacagcagtg aagcttgttt tgtgcagcca gccccgtttg
     1981 atcgaaaagg tgcagcgcag atggtcgccg ctcaacagca ccggcgcatc tgcagccaca
     2041 tcgttgatct ggcgttgcgg ctggctcgcc agcagtgtgc gggtataggc ttgctgcggc
     2101 gctgcgaaca atgcggcggt cgcagcggtt tcggccagcc tgccttgttc catcacgccg
     2161 acgcgatcag cgaaatgccg taccagattg aggtcatgcg agatcagcag caccgccatg
     2221 ttttcatcgc gctgcaactg gttcagcaac tccagtatct gcacctgaat cgtcacatcc
     2281 agcgctgtgg tcggctcatc ggcgatcagc agtctgggat ggcaggccag ggccatggcg
     2341 atcatcgcgc gctggcgctg accgcctgag agttgatgcg gataggccag ggtgagccgc
     2401 gcgggttcgg gaatgccagt tttttccagc agggcgaccg tgcgctgcgc tgctgtcttc
     2461 gcgctcaggc cctcatgcag catcagcact tcggcgatct gattgccgat ggtgaacaag
     2521 ggattcaggg ccgtcatcgg ctcctgaaaa atcattgccg ccgccatgcc gcgtatgccg
     2581 cgcatggcgg cttcgctctt ttgcaggatg tcttcgccgt tgaacagaat ctggccgcta
     2641 tagtgggcgt cctgattcag acgcagcacc gacagggcgc tgacggtctt gccggaaccg
     2701 gattcgccga ccagcgcaaa tttttcaccc ggcgcgatgg taaagctgac accatccacg
     2761 acagtcgcag cgccgaagcg taccgacaga ttgcggatgt cgagcaggct catgcttttc
     2821 tccgtacatc gagggcattg cgcaaggcgt caccgatatt ggtcagcagc agcagggtga
     2881 cgctcagcgc gatgaaggtc gacagggcga tccaccaggc atcgaggttg ttttttccct
     2941 gcgccagcag ttcgccgaga ctcggcatcg atggcggtac gccgagaccc agaaagtcga
     3001 gactggtcag cgccaggatg gccgcgctca tgcggaatgg caaaaacgtc accactggcg
     3061 tcaggctatt gggcaaaaca tgacgccaga tgatctgcat gttcgacaaa cccagcgcgc
     3121 gcgctgcctg cacatattcc agatggcgat tacgcagaaa atcggcgcgc acataatccg
     3181 acaggcccat ccagccgaac agtgacagca gcagtagtag caagcctatg cccggctcga
     3241 agatggaaga aaaaatgatc agcaggtaca gttccggcat ggctccccag atttccagga
     3301 tgcgttgcat gaacaaatcg gtacgcccgg caaaataccc ctgcgccgcg ccggccagca
     3361 aacccagcgc cacgccggtc accgtcaacg ccagaccgaa caggatggaa atacggaagc
     3421 catacagcag acgcgcgaac acgtcgcgtc cgcggtcgtc cgtacccagc cagttttcgg
     3481 aagaaggcgg cgccggattg ggttccctgg cgaaataatt gagtgtatcg aagcggtaac
     3541 gattgagcgg atagatagcc cagttgccgt ctctgtgcaa ctggtcctgg atgaaaggat
     3601 cgagataatc agccggcgag tcgaagtcgc cgccaaaggt tttctcggaa taatcgtgtg
     3661 caaccggaaa caggatctga ccgtgatagc tggcgatcag cggtctgtcg ttggacacca
     3721 gttcggcgac gagactgaac gcgaacagcg ccagaaaaat gatcaggctc cagtagccgc
     3781 ggcggctttg gcgaaagcgc agccagatac ggcggcccgg agaaaccggg ggggacagca
     3841 atgccgcgtt catccagcca gactttcgaa ctgtatgcgt gggtcggccc agacataaca
     3901 caggtcgccc agcagtttca cgaccagacc gatcaaggtg aacagataca ggcttcccat
     3961 caccaccgga tagtcgcggc ggatcaccga ctcataagac agcaggccga ggccgtcgag
     4021 cgagaacagt gtttcgatca gcaggctgcc ggcgaaaaaa gcgccgatga acgccgccgg
     4081 aaatccggtc accagcggaa tcagggcatt gcgcaacaca tgcttgtaca gcaccttgtt
     4141 ttcgctcaag cctttggccc ttgcggtcag cacatattgc ttgcggattt cttcgagaaa
     4201 gctgtttttg gtcaacatgg tcatcaccgc aaacgcgccg gtcactgagg cggtcaccgg
     4261 cagggtgatg tgccagagat aatccttgag cttgccgaac agcgacagcg cggcccagtc
     4321 atccgaagtc aatccgcgca atggaaacca ttgcacgaaa ctgccgccgc cgaacatcac
     4381 cagcagaaac actcccagta cgaatccggg aatggagtag ccaaccagca ctacggcgct
     4441 ggtcagcgcg tcgaaatggc tgcccgcgcg caccgccttg gcgatgccca atggcaccga
     4501 gactagatac gtcaggaaga aagtccacaa gccgagcgtg atcgacaccg gcatcttgga
     4561 caggatcagt tgccagacgt cgccgtgatg gtaaaaactc ttgccgagat cgaagctggc
     4621 aaatcctttg agcatgtgcc agaagcgctc gacgggcggc ttgtcgaaac cgtagagcgc
     4681 cttgatttca tcgatacgcc cggcgtcgat gccctgacgc gcgcgatatg gcgacgcgcc
     4741 gccgccagat gcctcaccgg caccgccgcg tcctttcatt tcgatcagcg cctgttccac
     4801 cggaccgccc ggtacgaact ggatcacggc aaaagtgatc gcgacgacgc cgaacaaggt
     4861 cggcagcatc aacaggatac gtttgaggat ataagaccag atgttcattt tggcgacttt
     4921 tcccaccagg tcgagatcac atacgggtcg gcctgataat acaaagggac aatggccggg
     4981 atgccgaagc gattgcggta cgccacacga tgcgtcgaag cataccactg tggcacgacg
     5041 atgtatttgt gcagcagtac gcggtccagt ccacgcgcgg cggcgaccag atccgcgcgc
     5101 gtgtcggcgg cgaccagcgc ttcgaccagt ttatcgatgg caggatcctt caggccccag
     5161 acattgttcg aacctttttc atcggcggcc ctggaaccga acatgtcgaa cagttcattg
     5221 cccggactgc tgacatccgg gaaacggata ctggtcatgt cgtaatcgaa ttcatccatg
     5281 cgcttggcga cgagcgcgta atcggcgctg cgctgcgtga cctgaatgcc aagcttttgc
     5341 agattgcgca cgtagatgct gatcacgcgc gacagcgccg actggtcatc gagaatttca
     5401 aaggcaaata cttcgctccc ggcattgcgc aaagcgccat cgcgatactc ccaaccagcc
     5461 tgacgcagca gctcccgcgc ttgcagcaga ttggcgcgca aggaagcagg cgggtcagtg
     5521 ctgggcggca ccggtgccgg gccgaagaca gccggatcga tacggatgcc agcggccctg
     5581 atcgactcca gcagcttcag ttcagcgccg gccggcacgc cggtggcggc cagatcgctg
     5641 ttattgaaga aagaataaat gcgcttgtac tggccgtaaa acaactggcg gttcatccat
     5701 tcgtagtcga gcgcgagtcc cagcgcccgg cgcacgcgcg ggtccctgaa tcgatcacgg
     5761 cgcagattca tcacgaaccc ctgcatgcca gcgccgttgg catgtttcag ttcgcgtttg
     5821 acgatctcgc cgctagtaaa tttggggccg ctatagttgc gcacccagtt cttcgcgctg
     5881 ttctcgacca caacgtcgaa
//
LOCUS       DASRDH010000285         2134 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_685329,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000285 DASRDH010000000
VERSION     DASRDH010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_685329"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1394
                     /locus_tag="VFA14_06655"
     CDS             <1..1394
                     /locus_tag="VFA14_06655"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HZS81088.1"
                     /translation="DGRCYIADAVERGAAAVIYEEVGFEWDGRFVLPHVGVTGLKRLA
                     GFVANAYYQQPDKDMFTVAITGTNGKTSCAYWLGMALQEAAAPAAVIGTLGIGMFRDG
                     KSCTFDTTGYTTPDAVLLQRRLAGLRAAHASVLAIEASSIGLDQGRMNGLHVDVALFT
                     NFTRDHLDYHGDMIAYETAKTRLFDVPGLRHAVINLDDQMGQRLIPDVQARNIPVIAY
                     SITASPTPVVAGDVPVLRASAIRSTNIGTSFHIASPFGVGQVKTQLVGPFNVSNVLGI
                     LGVLLAKGMDWDAALGAIATLTAAPGRMQQFGGQEVPLIVIDYAHTPDALEKTLIALR
                     QVASQRGGALWCVFGCGGDRDSGKRPEMGAVSELADHVILTSDNPRSEDPDVIILHIQ
                     SGMTRIVPHVMVDRATAILWAVRHALKQDVVLLAGKGHETCQEIGGRKYPFLDADHVA
                     LAMVARVTMMGGA"
     gene            1403..>2134
                     /locus_tag="VFA14_06660"
     CDS             1403..>2134
                     /locus_tag="VFA14_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007878123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HZS81089.1"
                     /translation="MQATLDQLQHWLDGAVVHHANGALTVRGVSTDSRAVAPGNLFVA
                     LRGERFDAHDFLDTVAAQGAAAVVVERIPAGCSIPAIVVPDTRIALGQIARHWRQQFV
                     IPVIAVTGSNGKTTVKEMIAAILSAAFGQERSMATYGNFNNAIGVPLTLFRLQPQHRA
                     AVVELGMNHTGEIAVLADIARPTLALVNNAQREHQEFMTTVEAVARENAAVFAALPDD
                     GIAVFPAEDDYAWVWRELAGKRPVLS"
BASE COUNT          402 a          614 c          659 g          459 t
ORIGIN      
        1 ctgatggccg ttgctatatt gccgacgccg ttgagcgtgg cgccgctgcc gtcatctatg
       61 aagaagtcgg gtttgaatgg gacggcagat ttgtattgcc gcatgtcggc gtgactggtt
      121 tgaagcgact ggccggcttt gtcgccaacg cttattacca gcagccggac aaggatatgt
      181 ttaccgttgc catcaccggc accaatggca agacttcctg tgcgtattgg ttgggcatgg
      241 cactgcaaga ggctgctgcg ccggctgccg tgatcggcac gctgggcata ggcatgttcc
      301 gggatggcaa atcatgcacc ttcgacacca ccggttacac cacgccggac gcggtgctac
      361 tgcaacgcag gctggctggt ctgcgcgcgg cgcacgcctc ggtgctggca atcgaagctt
      421 cgtccattgg actcgatcag ggccgcatga atggtctgca tgtcgatgtt gcgctgttta
      481 ccaatttcac gcgcgatcat ctcgactatc acggtgacat gatcgcttat gaaacggcca
      541 agacccggtt gttcgatgtg ccgggattgc ggcatgccgt catcaatctg gatgaccaga
      601 tggggcagcg tctgattcct gatgtgcagg ccaggaatat tccggtcatc gcttattcca
      661 tcactgcctc gcccacacct gtcgttgccg gcgatgtgcc ggtcttgcgg gcatctgcga
      721 tccgcagcac caatattggc accagctttc atatcgcttc accgtttggc gtgggccagg
      781 ttaagacaca gctggtcgga ccgttcaacg tcagcaatgt acttggcatt cttggcgtac
      841 tgctggccaa gggcatggat tgggatgcgg cgctgggtgc catcgccacg ctgactgctg
      901 cgccaggccg catgcagcaa ttcggcggac aggaagtgcc gctgatcgtg attgattatg
      961 cacacacgcc cgacgcactg gaaaaaacgc tgattgcctt gcgccaggtc gctagccagc
     1021 gcggtggtgc attatggtgc gtattcggct gtggcggcga tcgtgattct ggcaaacggc
     1081 cggagatggg cgcggtgtcg gaactggccg atcatgtgat tctgaccagc gacaatccgc
     1141 gcagcgaaga tccggatgtc atcattttgc atatccagag cggcatgacg cgcatcgtgc
     1201 cgcacgtcat ggtcgaccgt gctacggcca tcctctgggc ggtgcgccat gcgctcaagc
     1261 aggacgtggt attgctggcc ggcaaaggtc atgaaacctg tcaggaaatc ggcggcagga
     1321 aatatccttt cctcgatgct gaccacgttg cgctggccat ggtggcgcgc gtcacgatga
     1381 tgggaggcgc gtgatgacgt atttgcaagc cacgcttgat cagcttcaac attggctgga
     1441 cggcgctgtc gtgcatcatg ccaacggtgc gttgacggtg cgcggcgtca gtactgacag
     1501 ccgtgcggtg gcgcccggta atctgttcgt cgccctgcgc ggcgagcgtt tcgatgcgca
     1561 tgatttcctc gataccgtgg cagcgcaagg cgcggcagcg gtcgtggttg aacgcatccc
     1621 cgcaggatgc tctatcccgg ccattgtcgt acccgacacc cgcatcgctc tgggacagat
     1681 tgcgcgccat tggcgccagc aattcgtgat ccccgtgatc gctgtcaccg gcagcaacgg
     1741 caaaaccacc gtcaaggaaa tgatcgccgc catcctgtcg gctgcttttg gtcaagagcg
     1801 cagcatggct acgtacggca atttcaacaa tgcaatcggt gtgccgctga cgctgttccg
     1861 cctgcagccg caacatcggg cagcagtagt tgaactgggc atgaatcata ccggcgaaat
     1921 cgccgtgctg gccgacatcg ccagaccgac gctggcactg gtcaataacg cgcagcgcga
     1981 gcatcaggaa ttcatgacca cggtcgaggc ggtcgcgcgc gaaaacgctg ccgtttttgc
     2041 cgcattgccg gacgatggta ttgccgtatt cccggcagaa gacgactatg cctgggtctg
     2101 gcgcgagctt gccggcaagc gtcctgtact cagc
//
LOCUS       DASRDH010000286         5973 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1318316,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000286 DASRDH010000000
VERSION     DASRDH010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 5973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5973
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1318316"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2195
                     /locus_tag="VFA14_06665"
     CDS             <1..2195
                     /locus_tag="VFA14_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZS81090.1"
                     /translation="IDSVRGTLIEGAALTVLIVFLFLNSWRSTVITGLTLPVALIGTF
                     LFMYLFDFTINMITLMALSLCVGLLIDDAIVVRENIVRHAGMKAHGRFKTHRAAALEG
                     TAEIGLAVLATTLSIVAVFLPVGFMGGIIGRFFHQFGVTVTVAVLISMFVSFTLDPML
                     SSVWPDPEVHGERHRRDWYARTIGRVLAQFERLVQWLSATYQTLLMRSLRHRVLTLVI
                     ALLTFIAGLALPASGLVGSEFVPQADYSETGVTFYTPVGSSLELTETKVHQVETVLRQ
                     FSEVVDIYSTVNTGYAQGKNYAVVFIRLKPHEQRRLSMAQLSPLLRARLTQIAGITVT
                     HVGTLDGIGGDSKQVRFSLLGQNLDQLARLSDEVQARLRAIPGVVDLDSSMKAQKPTI
                     AIEPRRELGADLGIGVAQIGNALRPLLAGEVASSWRAPDDENYDVVVRLAPGDRNTID
                     DLSRLMLASAFNHADGTPRMVPLRQVVQISDSVGASQINRRDLSREIELSANTVGRSP
                     GQVNADIKSMLDGIDWPPGYRYQIGGSTRNMNESFGYAMGALVLAIVFIYMILASQFA
                     SFLQPLAIMSSLPLTLIGVFLALLSFRSTLNLFSIIGFIMLMGLVTKNAILLVDFANQ
                     ARREGMERGAALLEAARVRLRPILMTTLAMVFGMVPLAFGLGEGAEQRAPMGQAVIGG
                     IITSSLLTLVVVPVVYTYLDDLGAWVRRKCFSHAAGQDNRMNDIVDAG"
     gene            complement(2215..3222)
                     /locus_tag="VFA14_06670"
     CDS             complement(2215..3222)
                     /locus_tag="VFA14_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011402287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HZS81091.1"
                     /translation="MYTVQISFHILVLLIILALLFDFMNGFHDAANAIATVVSTGVLR
                     PQTAVMMAAFFNFAAVFVFHQLHVAASVGKGTIDPAVVDHVVVFGALVGAIFWNLVTW
                     YYGIPSSSSHALIGGLVGAAVAKAGTGALASSGLLKIIVFIVLSPLLGFVFGSFIMLL
                     VSWIFARSTPRKVDVWFRRAQLLSASMYSLGHGGNDAQKTIGIIWMLLIAGGYSSVDN
                     PPIWVTISCYCAISLGTLFGGWRIVRTMGQKITKLKPVGGFCAETGGAATLFLATAMG
                     VPVSTTHTITGAIVGVGASRKMSAVRWGVAGNIVWAWIFTIPASAFVAAIAWWIGMQI
                     F"
     gene            complement(3297..3923)
                     /locus_tag="VFA14_06675"
     CDS             complement(3297..3923)
                     /locus_tag="VFA14_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 domain-containing protein"
                     /protein_id="HZS81092.1"
                     /translation="MFGRWMPTEGKFFDLFNQHAELGVKCAAEMVALMTNFDHLEMRV
                     HAIEALEKQADKVTHNTIDQLRKTFITPLDRDDIHQLITRMDDILDLLEDAAQTVSLY
                     DIKTITPEAIRLAELCLACAEKVRTAVGLLCDMDNSRQILAVCDEIDHLEADADHVMH
                     AAMSKLFRDEPDVRNLIKLKAIYEILESVSDRCEDVANIIEGIVVENA"
     gene            complement(4012..5418)
                     /locus_tag="VFA14_06680"
     CDS             complement(4012..5418)
                     /locus_tag="VFA14_06680"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007882555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HZS81093.1"
                     /translation="MRAMNAPSDPQIESLRVPPHSIEAEQSVLGGLLLDNSAWDRIAD
                     FVNADNFYRYDHRIIFQHIVKLISETKPADVITVHEALSTSGKAEEVGGLNYLNALAQ
                     NTPSSANIRRYAEIVRDRGILRKLITVADEISGNAFQPQGKNVKQMLDEAESRIFAIA
                     EEGSRGLQGFMEIQPLLTQVVERIDELYNRDSGSDITGVPTGFIDLDRMTSGLQPGDL
                     IIVAGRPSMGKTAFSINIGENVAIDSGLPVAVFSMEMGGTQLAMRMLGSVGRLDQHRL
                     RTGRLNDEDWPRLTHAIQKMNDAQFYIDETPALNPIELRARSRRLARQCGKLGLVIVD
                     YLQLMSGNGGSNSGENRATEISEISRNLKSLAKELNCPVIALSQLNRALEQRPNKRPV
                     MSDLRESGAIEQDADVILFIYRDEVYNPDSQDKGTAEIIIGKQRNGPIGSIRLAFQGQ
                     FTRYDNYIGSPASPYGSD"
     gene            5453..5668
                     /locus_tag="VFA14_06685"
     CDS             5453..5668
                     /locus_tag="VFA14_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS81094.1"
                     /translation="MAAARPTGQVVAGAAVDDLFQVVIAVVAIAEDASLALRNREKDW
                     KMPPVTWRQEVSQFYVTMNASQKRSTA"
     gene            5757..>5973
                     /locus_tag="VFA14_06690"
     CDS             5757..>5973
                     /locus_tag="VFA14_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007879954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtA"
                     /protein_id="HZS81095.1"
                     /translation="MPSSSSCARLGLFQRWWFWGWLIVLAGGGYGYWHGGDARRGAAG
                     GDARGASPVGVALARTSDVRRYLTGLGS"
BASE COUNT         1184 a         1670 c         1830 g         1289 t
ORIGIN      
        1 gtatcgacag tgtgcgtggc acgctgatcg aaggagcggc gttgacggtg ctgatcgtgt
       61 tcctgtttct caattcgtgg cgttcgacgg tgatcaccgg actgaccttg ccggtggcgc
      121 tgatcggcac ctttttattc atgtacctgt tcgacttcac gatcaacatg atcacgctga
      181 tggcgctgtc gctgtgcgtg gggctgctga tcgacgatgc gatcgtggtg cgcgaaaaca
      241 tcgtgcgcca cgccggcatg aaggcgcatg gcaggttcaa gacgcacagg gcggcggcgc
      301 tggagggcac ggcggaaatc ggtctggcgg tgctggcgac gacgctctcc atcgttgcgg
      361 tattcctgcc ggtgggtttc atgggcggca tcatcgggcg cttcttccac cagttcggcg
      421 tgacggtgac ggtcgccgta ctgatttcga tgttcgtgtc gtttacgctg gatccgatgc
      481 tgtcgtcggt ctggcccgat cccgaggtcc atggtgaacg acatcggcgc gactggtatg
      541 cgcgtaccat cggccgcgtg ctggcgcagt tcgaacggct ggtgcaatgg ctgtcggcga
      601 cctatcagac gctgctgatg cggtcgctca gacatcgcgt gttgacgctg gtgatcgccc
      661 tgcttacttt catcgccggt ttggccttgc cggccagcgg actagtcggt tccgaattcg
      721 tgccgcaggc cgattactcc gaaaccggcg tgacttttta tacgcccgtc ggttcttcgc
      781 tggaattgac cgagaccaag gtccatcagg tcgaaaccgt gctgcgccag ttttccgaag
      841 tggtcgatat ctacagcacg gtcaacaccg gctacgcgca gggcaagaat tatgcggtcg
      901 tcttcatccg cctgaaaccg cacgagcagc gtcggctcag catggcacag ctgtcgccgc
      961 tgctgcgcgc ccggctgacg cagattgccg gtattacagt cacccatgtc ggtacgctcg
     1021 atggtatcgg cggcgacagc aagcaagtgc gtttcagctt gctgggacag aatctcgatc
     1081 agcttgcgcg tctgtcggat gaggtgcagg ccaggctacg cgcgattccc ggcgtggtcg
     1141 atctggactc cagcatgaag gcgcagaagc cgaccattgc catcgaaccc aggcgtgagc
     1201 tcggcgccga tctcggcatt ggcgttgcgc agatcggtaa tgcgctgcgg cctttgctgg
     1261 ccggggaagt cgccagcagc tggcgcgcgc ctgacgacga aaactacgat gtcgtcgtgc
     1321 gcctggcgcc gggcgaccgc aataccatcg acgatctgtc gcgtctgatg ttggccagcg
     1381 ctttcaatca tgcggacggt acgccgcgca tggtgccgct gcgtcaggtg gtgcagatca
     1441 gcgatagcgt cggcgccagc cagatcaacc ggcgcgacct gagccgcgaa atcgaactgt
     1501 cggccaatac cgtcggccgc tcgcccgggc aggtcaatgc tgacatcaag agcatgctcg
     1561 atgggataga ctggcccccc ggttatcgct accagatcgg cggctccacc aggaacatga
     1621 acgagtcctt cggctatgcc atgggcgcgc tggtgctggc catcgtgttc atctacatga
     1681 tcctggcgtc gcaattcgcc agcttcctgc aaccgctggc aatcatgtcg tcgctgccgc
     1741 tgacgctgat cggcgtgttc ctggcgctgc tgtcgttccg ctcgacgctg aacctgtttt
     1801 ccatcatcgg tttcatcatg ctgatgggac tggtgaccaa gaacgccatc ctgctggtgg
     1861 atttcgccaa tcaggcgcgc agggagggca tggagcgcgg cgcggcgctg ctggaagccg
     1921 cgcgtgtgcg gctgcgtccg atcctgatga ctacgctggc gatggtgttc ggcatggtgc
     1981 cgctggcctt tggccttggt gaaggcgccg agcagcgcgc gccgatggga caggccgtca
     2041 tcggcggtat cattacctcg tcgctgttga ccctggtggt ggtgccggtg gtgtatacct
     2101 acctggatga cctgggcgcc tgggtcaggc gcaaatgctt cagccatgcc gcaggacagg
     2161 ataatcgcat gaatgacatt gtcgatgccg gttgaaatac cggttgatgc gccgctaaaa
     2221 aatctgcatg ccgatccacc aggcgatcgc ggcgacaaac gccgatgccg gaatcgtgaa
     2281 aatccaggcc cagacgatat tgccggccac gccccaacgc accgccgaca tcttgcgcga
     2341 agcgccaacg ccgacaatgg cgccggtaat ggtatgtgtc gtcgaaaccg gtacgcccat
     2401 cgctgtcgcg aggaacagcg tggccgcgcc gccggtttca gcgcagaaac cgccgaccgg
     2461 cttgagcttg gtgattttct gtcccatggt tctgacgatg cgccatccgc caaacagggt
     2521 tcccaggctg atcgcgcaat agcaactgat ggtgacccat atcggtggat tgtcgacact
     2581 ggaataacct cctgcgatca gcagcatcca gatgatgcct atggtctttt gcgcatcgtt
     2641 gccgccatgg ccgaggctat acatggaagc tgacagcagc tgcgcgcggc gaaaccagac
     2701 gtcgaccttg cgcggtgtgg aacgcgcgaa aatccacgat accagcaaca tgatgaacga
     2761 gccgaacacg aatcccagca atggagaaag cacgataaag acgatgatct tgagcagacc
     2821 ggaagaagcc agcgcgccgg tgccggcctt ggccacggca gcgccgacca ggccaccgat
     2881 cagggcatgc gacgatgatg aaggaatacc gtagtaccac gtcaccagat tccagaaaat
     2941 cgcgccgacc agggcgccga acacgaccac atgatccacc acggccggat cgatggtgcc
     3001 tttgccaaca gaagccgcca cgtgtaattg atggaacacg aacaccgcgg caaaattgaa
     3061 gaatgccgcc atcatcactg ccgtctgcgg gcgcagcaca ccggtagata ccaccgtggc
     3121 gatcgcattg gcggcgtcat ggaaaccgtt catgaaatcg aacaggagcg ccagaatgat
     3181 cagcaggaca aggatgtgga aactaatctg aactgtatac atgaatggac tggactgagg
     3241 ggtatcggcg atatcacact gcgccggcgt tcggcgctgg cgcagtcgat gaaaaatcag
     3301 gcattttcga cgacgatgcc ttcgatgatg ttggcgacgt cttcgcaacg gtcggaaacg
     3361 gattccagga tttcataaat cgcttttaat ttgatcagat tgcgcacgtc cggctcatcg
     3421 cggaacagct tggacatcgc ggcgtgcatg acgtgatcag catcggcttc gaggtgatcg
     3481 atttcatcgc agaccgccag gatctgacgc gagttatcca tgtcgcacaa caggccgacg
     3541 gctgtcctga ctttctcggc gcaggccagg cacagctcgg cgaggcgtat ggcttccggc
     3601 gtgatggttt tgatgtcgta cagtgaaacg gtctgtgcgg cgtcttcgag cagatcgagg
     3661 atatcatcca tgcgcgtgat caattgatgg atgtcgtctc tgtccagcgg cgtgatgaag
     3721 gttttgcgca gttgatcgat cgtattgtgg gtgaccttgt ccgcctgttt ttccagagct
     3781 tcgatcgcat gcacacgcat ttccagatga tcgaaattgg tcatcaatgc gaccatttct
     3841 gcggcgcatt tcacgcccag ttcggcatgc tggttgaaaa gatcgaaaaa ttttccctca
     3901 gtgggcatcc agcgtccaaa catgttctac tctcttatga atgactgaaa caataagaat
     3961 cgtcctgtga tatcccggat tgtccatccc ggtgcatacc gcattgggcc atcagtcgct
     4021 gccgtaaggc gaagccggac taccgatgta attgtcgtac ctggtaaatt gcccctggaa
     4081 cgccaagcgg atactgccga tcgggccatt gcgctgcttg ccgatgatga tttcggcagt
     4141 tcctttatcc tgggagtcgg gattgtaaac ttcatcccgg taaatgaaca aaatcacgtc
     4201 cgcatcctgc tcgatggcgc cggattcacg caagtcggac atgacgggac gtttgttggg
     4261 gcgttgttcc agcgcgcggt tgagctggga taatgcaatc acggggcagt tgagttcctt
     4321 ggcgagactt ttcaggttgc gcgaaatttc ggaaatttca gtggcgcggt tttcaccgct
     4381 gttgctgccg ccattgcccg acatcagttg caggtaatcg acgatgacca gaccgagctt
     4441 gccgcactgg cgcgccagac ggcgtgagcg cgcgcgtaat tcaatcggat tcaatgccgg
     4501 tgtttcatcg atgtaaaact gcgcatcgtt catcttttga atcgcatgcg ttaagcgcgg
     4561 ccagtcttcg tcgttcaggc gtccggtgcg caaacgatgc tgatcgagac ggcccacgga
     4621 acccagcatg cgcatcgcga gctgggtgcc gcccatttcc atggaaaaca ccgcaacggg
     4681 caggccgcta tcgatggcga cattttcgcc gatgttgatg gaaaacgctg ttttacccat
     4741 tgaaggacgc cctgccacga tgatcaaatc tcccggctgc aaaccggatg tcatcctgtc
     4801 gaggtcgatg aaaccggtcg gcacaccggt aatgtcactg ccgctgtcgc ggttgtaaag
     4861 ttcgtcgatg cgctccacca cctgcgtcag caacggctgg atttccatga agccttgcag
     4921 gccgcgcgag ccttcttccg cgatcgcgaa aatcctggat tccgcttcgt ccagcatttg
     4981 cttgacgttc ttgccttgcg gctggaaggc gtttccagag atttcgtcgg caaccgtaat
     5041 cagtttgcgg agaatgccgc ggtcccgcac gatctcggca tagcggcgga tgttggcgga
     5101 cgacggtgtg ttctgcgcca gggcattgag atagttcagc ccgccgacct cctctgcctt
     5161 gccgctggtc gatagcgctt catggacggt aatcacgtcc gcaggtttgg tttcgctgat
     5221 cagcttgacg atatgctgaa aaataatgcg gtgatcgtag cgatagaaat tgtcggcatt
     5281 gacgaagtca gcgatacggt cccatgccga gttgtcgagc aacaatccgc ccagaacgga
     5341 ttgctcggct tcgatcgaat gcggcggaac gcgcagggat tctatttgcg gatcggaggg
     5401 ggcgttcatg gcgcgcatta tacctgttaa aagtggccga gccagttcga gtttggccgc
     5461 agccagaccg accggccagg tggttgccgg tgccgcggtc gatgacctgt ttcaggtagt
     5521 cattgccgta gtcgcgatag cggaagatgc gtctctggcc ttgcgcaata gggagaaaga
     5581 ttggaagatg ccgcctgtca catggaggca ggaagtcagc cagttttacg ttaccatgaa
     5641 cgcttcgcag aagcgatcga cagcatgact gcattgacca ttaattacgc gcggggacaa
     5701 gtgacttgga gaacgtgttc acgatgacta ccataccgac ttccagcgat cagcaaatgc
     5761 catcttcctc ttcttgcgcc aggttggggc tgttccagcg ctggtggttc tggggttggc
     5821 tgatcgtcct ggccggtggc ggctacgggt attggcatgg gggcgatgcg cggcgtggcg
     5881 ctgcaggggg ggatgccagg ggcgcatcgc cggtcggcgt ggcgctagcc aggacgtctg
     5941 atgtgcgcag gtatttgacc ggcctgggca gcg
//
LOCUS       DASRDH010000287         2531 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_990388,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000287 DASRDH010000000
VERSION     DASRDH010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_990388"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1205)
                     /locus_tag="VFA14_06695"
     CDS             complement(<1..1205)
                     /locus_tag="VFA14_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041322210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HZS81096.1"
                     /translation="MKYTDLRDFISQLQHAGELKRISVPVSSYLEMTEICDRTLRSSG
                     PALIFDKVDEKNIPVLANLFGTPRRVALGMGAENVSELRRIGHLLAQLKEPEPPRGFK
                     DVMGLGTLVKSLWDMVPREQRSAPCQDIVWQGNDVDLDRLPIQHCWPGDVAPLITWGL
                     VITKGPDKKRQNLGIYRQQVIGRNKVIMRWLAHRGGALDFREHGIVSPGKPYPLAVAF
                     GADPATILGAVTPVPDSLSEYQFAGLLRGSRTELVKAIGSELRVPASAEIVLEGHIYP
                     DEAHPSGYEHALEGPYGDHTGYYNEQDWFPVFTIDRITMRRDPIYHSTYTGKPPDEPA
                     VLGVALNEVFIPLLQKQFSEITDFYLPPEGCSYRMALVQMKKSYAGHARRVMFGVWSF
                     LRQFMYTKF"
     gene            1422..2342
                     /locus_tag="VFA14_06700"
     CDS             1422..2342
                     /locus_tag="VFA14_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HZS81097.1"
                     /translation="MPNQPIGAQLNASNTVDQSASWAKLTPGKVHRLLTSVQYAVTLL
                     GITALFVLGLMFFKPGLGDKLIALSPFSANASERIAAQAPPLQNLMTATANAIAVTQT
                     TTEEHKILGTPHHQQKLVTSWLSKRYRVASDAIDMLVSAAYITAHDIKLDPLLILAVM
                     AIESRFNPFAESPMGAQGLMQVMSKVHHDKFQQLGGVQAALNPVVNIKVGSLILKEYV
                     TRGGSVEAGLKSYVGAAGRKSDGGYGLKVLSEYWNLKQVAIGKNVSIFTATPSRPRLP
                     PPAAKPVAVDNIAAATPKKPDNTIEERVAL"
     gene            complement(2404..>2531)
                     /locus_tag="VFA14_06705"
     CDS             complement(2404..>2531)
                     /locus_tag="VFA14_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007879027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HZS81098.1"
                     /translation="RLKNSADAYTPRPDYRPVTKFENRGLRLGHGVWDLVFLRQP"
BASE COUNT          615 a          720 c          654 g          542 t
ORIGIN      
        1 atgaacttgg tatacatgaa ctggcgcagg aaactccaca cgccgaacat cacgcgtctg
       61 gcatgaccgg cgtaagattt cttcatctgc accaacgcca tgcggtaact gcatccctcg
      121 ggcggcagat agaaatcggt aatttcgctg aactgtttct gcagcagcgg aatgaacacc
      181 tcattcaacg cgactcccaa tacagccggc tcgtccggcg gtttacccgt gtatgtggaa
      241 tggtaaatcg gatcgcgccg catagtaata cggtcaatgg taaagaccgg gaaccagtcc
      301 tgctcattat aatagccagt gtggtcgccg tatggtcctt ccagcgcatg ctcatagccg
      361 gacggatgcg cctcatccgg gtaaatatgc ccttccagca caatctcggc cgaggccggt
      421 acgcgcaact cactgccaat ggccttgacc agctcggtgc gactgccgcg cagcaggccg
      481 gcaaactgat attcggacag gctgtcgggt accggcgtca ccgcgcccaa tatcgttgca
      541 ggatcggcgc caaacgcgac cgcaagcgga tacggcttgc ccgggctgac aataccgtgc
      601 tcacgaaaat cgagcgctcc accacgatgc gccagccagc gcatgatgac cttattgcgg
      661 ccgatgactt gctggcgata gatgccaaga ttctgacgct tcttgtcagg gcctttggtg
      721 atcaccagtc cccaggtaat gagcggcgcc acatcaccgg gccagcaatg ctgaatcggc
      781 aagcgatcga gatcgacgtc gttcccctgc cagacgatat cctggcaggg ggcggaacgc
      841 tgctccctgg gcaccatgtc ccacaatgac tttaccagtg tgcccagccc catgacatcc
      901 ttgaaccccc tgggtggttc cggctctttc aactgcgcca gcaagtggcc gatgcgcctt
      961 aattcactga cattttccgc gcccatgccc aatgctacac ggcgcggcgt accaaaaaga
     1021 tttgccaaaa cagggatgtt tttctcatcg actttatcga aaatcaacgc tggtccagag
     1081 ctacgcaacg tgcgatcgca aatttccgtc atttccaaat aggaagaaac aggcacggag
     1141 atacgcttca actctccggc atgttgtaat tgggaaataa aatcgcgtaa atctgtgtat
     1201 ttcatttttc tctggttttt tgaagtcgcg gtcttaaatg aggtagaccc ggcaaatggt
     1261 tgattttatt gattttcaaa gggatatccc tccaataatc atacttataa gttatataaa
     1321 ttacattcaa tggtattgac ttgatataag gtggcttcta caattcgcga aactttgaga
     1381 gaaagagcgg ggtcgaaaac ccgaatcagc taggaggttc aatgccaaac caacccattg
     1441 gggcgcagct aaacgcctcg aatacggttg accagtctgc gtcatgggca aagctcaccc
     1501 cgggcaaggt ccaccgcctt cttacttcgg tgcaatatgc cgttactttg ctcgggatca
     1561 cggccttatt cgtattgggc ctgatgtttt tcaagcccgg tctcggcgac aagctcattg
     1621 ctctgtctcc cttctcggcc aatgcgtcag aaagaatcgc ggcgcaagcg ccaccactgc
     1681 agaatctgat gaccgcgaca gcaaacgcca ttgcagtaac acagacgacc acggaggaac
     1741 acaagatcct gggtacgccg caccaccagc aaaagctggt caccagctgg ctgtcgaaac
     1801 gctaccgcgt tgccagcgat gcgatcgata tgctggtatc ggccgcctat atcacggcgc
     1861 acgacattaa actggatcca ctgctgatcc tcgcggtgat ggcaattgaa tcgcgcttca
     1921 atccatttgc cgaaagcccg atgggcgcgc aaggcttgat gcaggtcatg tccaaagtcc
     1981 atcatgacaa gttccagcaa ctgggtggcg tgcaagcggc gctcaatccg gtcgtcaaca
     2041 tcaaggtcgg ttcgctgatc ctgaaagaat acgtgacacg cggcggttca gtcgaagccg
     2101 gtctgaagag ctatgtcggc gccgctggca gaaagagcga cggcggctat ggcctgaagg
     2161 tcttgtccga gtactggaac ctgaagcaag tcgccatcgg caagaatgtc tcgatcttca
     2221 cggctacacc gtccagaccg cgattaccac ctccagctgc caaaccagtc gcagtcgaca
     2281 atatagcagc ggcaacaccg aaaaaaccag acaacaccat cgaagaacgc gtcgcgctgt
     2341 aatgctgcgt gccagcggct ggcctggtga gaatgtcatc aactttgaag atatgcgttt
     2401 tgctcaaggt tgcctgagaa ataccagatc ccagacgcca tggccaagac gcaggcctcg
     2461 gttctcaaac ttggtgacgg gacgatagtc cggccgcggc gtataagcgt ccgcgctatt
     2521 cttcagcctt g
//
LOCUS       DASRDH010000288         6277 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_629116,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000288 DASRDH010000000
VERSION     DASRDH010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 6277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6277
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_629116"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="VFA14_06710"
     CDS             complement(<1..534)
                     /locus_tag="VFA14_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015603558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HZS81099.1"
                     /translation="MPGKFKRQLSLTDLTFIGLGATFGSGWLFAASHVSAIAGPAGIL
                     SWLLGGFAVLLLGIVYCELGAALPRAGGIVRYPMYSHGPLLGYLMGSITLIAFSSLIA
                     IEVIASRQYAAAWFPGLTRTGSGDPTTLGWVFQFALLCLFFYLNYHSVKTFARANNFI
                     SLFKFVVPLLVISVLFFF"
     gene            complement(1106..1333)
                     /locus_tag="VFA14_06715"
                     /pseudo
     CDS             complement(1106..1333)
                     /locus_tag="VFA14_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020831613.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(1460..5206)
                     /gene="metH"
                     /locus_tag="VFA14_06720"
     CDS             complement(1460..5206)
                     /gene="metH"
                     /locus_tag="VFA14_06720"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HZS81100.1"
                     /translation="MKSNDPSQTELSLRDLMSKRILILDGAMGTMIQQYRLTEEDYRG
                     ERFKDFSVPGKALFIKGNNELLSLTQPHIIQEIHEKYLAAGADLIETNTFGATSVAQD
                     DYHMAHLVHEMNVQSARLARAACDRHATPDRPRFVAGALGPTPKTASISPDVNDPAAR
                     NITFDQLVTSYLEQTRALVEGGADVLLVETVFDTLNCRAALFAIDMFFEESGKRLPLM
                     ISGTVTDASGRILSGQTVPAFWHSIRHARPLTVGLNCALGAALMRPYAEELSRIADTF
                     VCIYPNAGLPNPMSDTGFDETPDVTSALLKDFAESGFINAAGGCCGTTPAHIKAIADT
                     VAGIAPRKVPQGRHDMLLSGLEPFTIDGDSLYVNVGERTNVTGSRAFARLILDGQYDA
                     ALAVARQQVENGAQIIDINMDEAMLDSVAAMTRFLNLIASEPDIARVPVMIDSSRWPV
                     IEAGLKCVQGKSIVNSISLKEGEEKFLHQARLCRRYGAAVIVMAFDEAGQADTFARKI
                     DICGRAYRLLVDEAGFPPEDIIFDPNIFAIATGIEEHNNYAVDFIEATRWIHQHLPYA
                     KISGGVSNVSFSFRGNDPAREAIHTVFLYHAIKAGMTMGIVNAGMIGVYDDLPAGLRE
                     AVEDVVLNRRPDATERMIDIAATLKAGDRKEEATLEWRSEPVEKRLAHALVHGITQWI
                     VEDTEAARQQLLQSGGRPIHVIEGPLMDGMNIVGDLFGQGKMFLPQVVKSARVMKQAV
                     AHLIPFIEEEKRRIEQETGVVSRPKGKIVIATVKGDVHDIGKNIVAVVLQCNNFEIVN
                     MGVMVPCSQILAKAKEENADIIGLSGLITPSLEEMAYVAREMQRDNYFSGVRMPLLIG
                     GATTSRAHTAVKIAPHYAGPVVYVPDASRAVAVAQALLTPEQKEQYIADMAADYERIR
                     TQHANRKATPLVSIAAARANKMQLQFAPKQPKFIGRRVFKNVDLATLARYIDWGPLFQ
                     TWDLAGPYPAILTDAVVGAAATKVFEEAQMLLKKIIDGRWLTANGVVMLLPANTVNDD
                     DIEIYADDSRTQPVLTYYGARQQSEKPVIDGIARPNQCLADFIAPKESGITDYIGMFA
                     VTAGLGIEKYEKRFQDTHDDYSSIMLKALADRLAEAFAEYLHQRVRTDLWGYAPQESL
                     TVEKLIKEKYSGIRPAPGYPACPEHTVKAEAFKLMQCDEIGMGITESHAMFPGASVSG
                     FYFAHPESRYFVVGKIGADQVADMARRRGVAGKDIERWLAPNLS"
     gene            complement(5203..6195)
                     /gene="fmt"
                     /locus_tag="VFA14_06725"
     CDS             complement(5203..6195)
                     /gene="fmt"
                     /locus_tag="VFA14_06725"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HZS81101.1"
                     /translation="MKIIYAGTPEFAAVALDALYQAGHDITLALTQPDRRAGRGMQLQ
                     ASPVKQRAIAYGIPVAQPLSLRLDGNYPDSARAAHQLLRDTPHDLMIVAAYGLILPRS
                     VLDIPRFGCINIHASLLPRWRGAAPIQRAIEAGDSETGVTIMQMEEGLDTGPMLLMAS
                     VPITSDDTTGSLHDRLAALGGRMIVEAVKKMEAGPLPATPQPEAGVSYAAKISKQEAV
                     LDFKQPAETLVRKIRAFHPFPGASATFGDGDVTVKLWQAQALPEKTDAAPGSILAANA
                     RDGVKVACGTGVLQITVLQKPGGKRLAAAEFLKACALEGGAFQLHQIPPRKTTP"
BASE COUNT         1272 a         1806 c         1830 g         1369 t
ORIGIN      
        1 gaaaaaaaac agcacgctga tcaccagcaa gggcaccacg aacttgaaga ggctgatgaa
       61 attattcgct ctggcgaagg ttttgacgct gtgatagttc aggtaaaaga acaggcataa
      121 cagcgcaaac tggaacaccc agcccaatgt cgtcggatcg cctgagccag ttcgggtcag
      181 acccggaaac catgctgctg cgtactggcg cgaggcgatc acctcgatcg caatcaggct
      241 ggagaaagcg atcaatgtga tcgaccccat cagatagccc agcaatggac catgcgaata
      301 catgggatag cgcacaatac cgcccgcacg cggcaacgct gcgcccagct cacaatagac
      361 gatgcccagc aacaataccg caaaaccacc cagcagccag gacaagatgc ctgccggccc
      421 cgcgatggcg gacacatgac tggccgcgaa cagccacccc gaaccaaaag tagcgcctaa
      481 accaatgaag gtcagatcgg tcagcgataa ctgcctcttg aatttgcctg gcataaagtc
      541 tcctcatatt ctactgatgg aatgacgcat cgccccaggt ttcgtcacga caggcagcaa
      601 gggattattt tttcttgttg atcgcctttt cgatttcctc gggcggaatt ttcagtgttt
      661 tggcaatctc ttccgttgtc atattttttt ccagcaagag caagagtttg atagctatcg
      721 cttttgcttc tttggccgcc tgtttagcaa tttctttcgc ttcttcttta gccgcttttt
      781 cagctttgag ggcctgtttg gcagcgagaa gctcttcttt gacttaaaag gttgggacat
      841 atttgaggct ggcagccagc ttgtccatca gtccaatcat atggaaactc agacactcct
      901 tcgtcgagat gatcagatga tctgccatcg tgatacccag gatgcggcca acgctgcatg
      961 atgccgtaga catcacccga accggcgatc tgcttcttgt ctttatcggt gagtctgatt
     1021 tgcatggcgc agggaacgct gaataagagc aggtggcgtt caatataaca cagcgcccca
     1081 tcgatatgga gggagaaatt cttgatcaaa taatcgcctg caagccctta tcggcaatga
     1141 cattgagcaa tttgagcgcc ggcccggtcg gatgcgtttc gccttgctcc cacttacgca
     1201 cggtcgaagc cgtagtgtgc agatgaagag cgaacacagg ttggctgaat ttcagctttt
     1261 tccgtaagcg cttgatgtcc gcagcgccaa actcccgtac caagggcggg caaattgcat
     1321 caaattcgcg catattatac ccgttacggg tatatttctt aagttatcca gggaataaga
     1381 caagcaacac aacgcttgcc aggaaaccga ggtgcattcc cggatcgacc aggcgataca
     1441 aagagattgt ttgaaatgtt caggacaggt tcggcgccag ccagcgctct atgtctttcc
     1501 cggcaacgcc gcggcgccgc gccatgtcgg cgacctgatc cgcgccgatc ttgccgacca
     1561 cgaaatacct ggactccgga tgcgcgaaat aaaaccccga caccgaagcg cccgggaaca
     1621 ttgcatggga ctccgtgatg cccatgccga tttcgtcgca ttgcatgagc ttgaaggctt
     1681 cagccttgac cgtgtgttcc gggcaggctg gataaccggg ggcaggacga atgccgctgt
     1741 atttttcctt gatcagtttt tcaaccgtca atgactcttg cggcgcatag ccccataaat
     1801 cggtacgcac acgctgatgc aggtattccg caaatgcttc ggccagacgg tcagccagcg
     1861 ccttgagcat gatcgaagaa taatcatcat gcgtatcctg aaaacgtttt tcatatttct
     1921 cgatgccgag tccggcggtc acggcaaaca tgccgatgta atcggtgatg ccggattctt
     1981 tcggtgcgat gaagtcggcc agacattggt ttggacgggc aatgccgtcg atcaccggct
     2041 tttcgctctg ctgacgcgcg ccgtagtaag tcagtacagg ctgcgtgcgg ctatcgtcgg
     2101 catagatttc gatatcgtcg tcgttgacgg tattggccgg cagcagcatg acgacgccgt
     2161 tggcggtcag ccagcggcca tcgatgattt ttttgagcag catctgcgct tcttcgaata
     2221 ccttggtcgc ggcggcgccg acgacggcat cggtcaggat ggcgggatat ggcccggcca
     2281 gatcccaggt ctgaaacaac ggaccccagt cgatatacct ggccagcgtc gccagatcga
     2341 cgttcttgaa cacgcggcga ccgatgaact tcggttgctt cggcgcaaat tgcagttgca
     2401 tcttgttggc gcgcgcggcg gcgatcgata ccagcggcgt cgctttcctg ttggcgtgct
     2461 gcgtgcggat gcgttcgtaa tcggccgcca tgtcggcgat gtactgttct ttctgttccg
     2521 gcgtgagcag cgcttgcgcg accgccacgg cgcgcgaggc atccggcacg tagaccaccg
     2581 gacctgcgta atgcggtgcg atcttgacgg cggtatgcgc gcgtgaggtg gtcgcgccgc
     2641 cgatcaacag cggcatcctg acaccgctga aatagttgtc acgctgcatt tccctggcga
     2701 cgtaggccat ttcttcgagc gatggcgtga tcaggccgga caggccgatg atgtcggcat
     2761 tttcttcctt ggccttggcc aggatctgcg agcacggcac catcacgccc atgttgacga
     2821 tttcgaagtt gttgcattgc aggaccaccg cgacgatatt cttgccgatg tcatggacgt
     2881 cgcctttgac ggtggcgatg acgatcttgc ctttgggcct ggagacgaca ccggtttcct
     2941 gctcgatccg gcgcttttct tcttcgatga agggaatcag atgcgccacg gcctgcttca
     3001 tcacgcgcgc cgacttgact acctgcggca aaaacatttt tccctggccg aacaggtcac
     3061 cgacgatgtt catgccgtcc atgagcgggc cttcgatcac gtggatcggc cggccgccgc
     3121 tctgcaagag ctgttggcgc gctgcttcag tatcttcgac tatccattgc gtgatgccat
     3181 gtaccagcgc gtgcgccaga cgtttttcga ccggctcgct gcgccattcc agcgtggctt
     3241 cttcttttct gtcaccagcc ttgagcgtag cggcaatgtc gatcatgcgc tcggtcgcat
     3301 ccggacggcg gttcagcacc acgtcttcga cggcttcgcg cagcccggca ggcaagtcgt
     3361 cgtagacacc gatcataccg gcattgacga tgcccatggt catgccggct ttgatggcgt
     3421 gatacaggaa gacggtgtgg atcgcttcac gcgccggatc gttgccgcgg aagctgaagc
     3481 tgacgttgga tacgccgccg ctgattttgg catacggcag atgttgatga atccagcgcg
     3541 tcgcctcgat gaaatcgacg gcgtaattgt tgtgctcttc gatgccggtc gcgatcgcga
     3601 aaatgttcgg gtcgaagatg atgtcttccg gcggaaagcc agcctcgtcg accagcaggc
     3661 gataggcgcg cccgcagatg tcgatcttgc gcgcaaaggt atcggcctga ccggcttcgt
     3721 cgaaagccat gacgatcacc gcagcgccgt agcggcggca cagtctggcc tggtgcagga
     3781 atttctcttc gccttccttg agtgagatag agttgacgat ggacttgccc tgcacgcact
     3841 tgaggccggc ttcgatgact ggccaccggg atgaatcgat catgacaggc acacgggcga
     3901 tgtccggttc cgatgcgatc aggttgagga agcgtgtcat ggcggcgacc gaatcgagca
     3961 tcgcttcgtc catgttgatg tcgatgatct gggcgccgtt ctcgacctgc tggcgcgcga
     4021 cggcaagcgc tgcatcatat tgcccgtcga gaatcagacg ggcaaaagcc ctggaaccgg
     4081 tgacgttggt gcgctcgccg acattcacgt acagcgagtc gccatcgatg gtgaagggtt
     4141 ccaggcccga caacaacata tcatgcctgc cttgcggcac cttgcgcggc gcgatgccgg
     4201 cgacggtatc ggcaatcgcc ttgatatgcg ccggcgtagt gccgcagcag ccgccggcgg
     4261 cattgatgaa accgctctcg gcaaaatctt tcagcaacgc cgacgtgacg tccggcgtct
     4321 cgtcgaagcc ggtgtcgctc atcggattgg gcaagcccgc attcggataa atacagacga
     4381 aggtgtccgc gatccgggac aattcttctg catagggacg catcaacgcc gcgccgagcg
     4441 cgcagttcag gccgacggtc agcggacgcg catggcggat cgaatgccag aaggctggta
     4501 cggtctggcc ggataaaata cgtcccgagg catcggtgac ggtgccggag atcatgagcg
     4561 gcaggcgctt gcccgattct tcgaagaaca tgtcgatggc aaataacgcg gccctgcagt
     4621 tgagcgtgtc gaaaacggtc tcgaccagca gcacgtccgc gccgccctcg accagtgcgc
     4681 gcgtctgctc cagatacgag gtcaccagtt gatcgaaggt gatattgcgc gcagcgggat
     4741 cgttgacgtc cggcgagatc gaagcggtct tcggtgtcgg gccaagcgcg ccggcgacga
     4801 aacgcggcct gtccggcgtc gcatgtctgt cgcaggccgc acgcgccagt ctggccgact
     4861 gcacgttcat ctcatgcacc agatgagcca tatgatagtc atcctgcgcc acgctggtgg
     4921 cgccgaaggt attggtttcg atcaggtcgg cgccggccgc caggtatttt tcatggattt
     4981 cctggatgat gtgcggctgg gtcaggctca acagctcatt gttgcccttg atgaacaagg
     5041 cttttcccgg cacgctgaaa tccttgaagc gctcgccgcg atagtcctct tccgtcagcc
     5101 ggtattgctg gatcatggtt cccatcgcgc catccagaat caggatgcgt ttcgacatca
     5161 ggtcgcgtaa cgacaattcg gtttgggacg gatcgtttga cttcatggtg tcgtttttcg
     5221 tggtggaatc tggtgtaact ggaaagcgcc gccttccagc gcgcaagcct tgagaaattc
     5281 cgccgcagcc aggcgcttgc cgccgggttt ctgcaatacc gtgatctgca acacgccagt
     5341 accgcaagcg actttcacgc catcgcgcgc gtttgcggcg agaatacttc ccggcgcggc
     5401 gtccgtcttt tccggtaaag cctgcgcctg ccacaatttg accgtgacat cgccatcgcc
     5461 aaatgtggcc gacgcgcccg ggaacggatg gaaagcacgg attttgcgca ccagcgtttc
     5521 cgccggctgc ttgaaatcga gtacggcttc ttgcttgctg atcttggcgg cgtaactgac
     5581 gcctgcttcc ggctgcggcg tcgccggcaa gggaccggct tccatttttt tcactgcctc
     5641 gacgatcatc ctgccaccca gagccgccag cctgtcgtgc agactgccgg tggtgtcatc
     5701 gctggtaatc ggcacggatg ccatgagcag catcggacca gtgtccagcc cttcttccat
     5761 ctgcatgatg gtcacgccgg tttcgctgtc gccggcttca atggcacgct ggatcggcgc
     5821 cgcgccgcgc cagcgcggca gcagcgaagc atggatgttg atgcagccga aacgtggaat
     5881 gtccagcaca ctgcgcggca gaatcaagcc ataggcagca acgatcatca gatcgtgcgg
     5941 cgtatcgcgc aacagttgat gcgctgcgcg cgcactgtcg ggatagttac cgtccagacg
     6001 caaggacagt ggctgcgcta ccggtatgcc atacgcaatg gcgcgttgct tgactggcga
     6061 agcctgcaac tgcatgccgc gcccggcgcg acgatccggc tgggtcagcg ccagggtgat
     6121 gtcatggccg gcctggtaga gggcatcgag ggcgacggcg gcgaattccg gtgttccggc
     6181 gtagatgatt ttcatcgtga caacatggga caatgccagt gcggcagcac tgccagatca
     6241 actcaccgtc gaagccgcac actatatatg ttgtagc
//
LOCUS       DASRDH010000289         3798 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1211750,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000289 DASRDH010000000
VERSION     DASRDH010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 3798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3798
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1211750"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1226)
                     /locus_tag="VFA14_06730"
     CDS             complement(<1..1226)
                     /locus_tag="VFA14_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZS81102.1"
                     /translation="MLAWFEKLVYPYPDTMTKPPPRGFLLFIWACTKGLRRYIIGMTL
                     LTAVIGAFEALLFVMMGHIIDWLAHVEPAQLWVQERSSLIWLALVLTGSPVLIALQTF
                     LKHQTLSGAFPMMLRWKFHRLMLNQSMSFYQDEFAGRVATKVMQTALAVRDVCMIIGD
                     ILVFVMIYFITMTAVVGNFDAWLLLPFFGWLLLYIAALRYFVPRLSKVARNQADARSL
                     MVGRITDAYTNIATVKLFSHAGREAAYARSAMQDFLLTVHRQMRLISGFEIVNHTLNM
                     MLVLSTAGIALWLWTQAQVGVGAVAAATAMALRLNGLAHWVMWEMAILFEHVGTVQDG
                     INTLSRAHEVNDLPDAQPLQAKHGELRFENVSFAYGAAGPHARQVIDKLDLHIQPGEK
                     IGLVGRSGAGKSTIVNL"
     gene            1453..1725
                     /locus_tag="VFA14_06735"
     CDS             1453..1725
                     /locus_tag="VFA14_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007882515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein"
                     /protein_id="HZS81103.1"
                     /translation="MPLPDKESLIEYPSDFPIKVIAIMHDEFVNHIVEMITQHDPTFH
                     AGKVEIRPSSQGKYLSLTVTVLATSRAQLDNLYRALSSHEMVKFVL"
     gene            1732..2388
                     /gene="lipB"
                     /locus_tag="VFA14_06740"
     CDS             1732..2388
                     /gene="lipB"
                     /locus_tag="VFA14_06740"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HZS81104.1"
                     /translation="MQAVPQIIRRGIEDYQASFDAMRAYTDNRTPDTHDQLWIVEHPS
                     VFTLGLGADIAHILDPHAIPVIQTDRGGEVTYHGPGQVVIYLLMDLRRHHSGARLLVR
                     EFVQKIEQAVIDTLATYNLAGERKQGTPGIYVSTGRWQGAKIAALGLKIRSNACTYHG
                     VSLNVAMDLTPFSWINPCGHAGLATVDMRTLGAAAEPSEVQTLLTDRLVEYFVLPPSA
                     "
     gene            2405..3379
                     /gene="lipA"
                     /locus_tag="VFA14_06745"
     CDS             2405..3379
                     /gene="lipA"
                     /locus_tag="VFA14_06745"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HZS81105.1"
                     /translation="MMMTTNIAPTKALRNPADKQKGAGKTAWIPIKVMPAEKLKKPDW
                     IRVKAGSPTTRFYEIKDILRASNLVTVCEEASCPNIGECFGKGTATFMIMGDKCTRRC
                     PFCDVGHGRPDPLDPNEPENLARTIAQLKLSYVVITSVDRDDLRDGGATHFTDCIQRV
                     RELSPTTRIEILTPDFRGRIDRALEIFKTAPPDVMNHNLETAPRLYKEARPGSDYEYS
                     LNLLKRFKELHPDTPTKSGIMVGLGETDEEVLQVMRDMRAHDVDMLTIGQYLMPSNDH
                     LPVRRYVHPDTFKMYEEEACKMGFMHAAVGAMVRSSYHADQQAHGLVG"
BASE COUNT          959 a         1020 c          935 g          884 t
ORIGIN      
        1 agattgacga tggtcgattt gccagcgccg gagcgaccga ccagaccgat tttttcacca
       61 ggctgaatgt gtaagtcgag tttatcgatc acctggcgcg catgtggacc ggctgcgccg
      121 taagcgaagc tgacattttc aaaacgcaat tcgccatgtt tggcctgtag cggttgtgca
      181 tcgggcaagt cgtttacttc atgcgcgcgc gatagcgtat tgatgccgtc ctgtacggtg
      241 ccgacgtgtt cgaatagtat cgccatttcc cacatgaccc aatgggcgag gccgttcagg
      301 cgtaacgcca tggcagttgc cgctgccacc gcgccaacac cgacctgagc ttgcgtccat
      361 agccacaagg ctatgcctgc ggtcgataga accagcatca tgttgagcgt atgattgaca
      421 atttcgaaac cactgatcag acgcatctgt cgatgcaccg taagcagaaa atcctgcatc
      481 gcgctgcgtg cataagcggc ttcgcgtcca gcgtgtgaaa acagtttgac ggtggcgata
      541 ttggtgtagg catcggtgat acgaccaacc atcagtgaac gcgcgtcggc ttggttacgt
      601 gcgaccttcg acaggcgtgg cacgaagtag cgcagcgcgg cgatatacaa cagcagccag
      661 ccaaagaagg gcaacagcag ccacgcatca aagttgccga ccaccgccgt catcgtgatg
      721 aaatagatca tcacgaacac caggatgtca cctatgatca tgcagacgtc gcgtaccgcc
      781 agcgcggttt gcattacctt ggtggcgacg cgtccggcaa actcgtcctg atagaaactc
      841 atgctctggt tgagcatcag gcgatggaat ttccagcgta gcatcatcgg aaacgcacct
      901 gacagggttt gatgcttgag gaaagtctgc aatgcgatca atactggact gccggtcagc
      961 accagtgcca gccatatcag gctgctgcgt tcctgcaccc acaattgcgc cggttcgaca
     1021 tgcgcgagcc agtcgatgat atggcccatc atgacgaaca acagcgcttc gaatgcgccg
     1081 atgaccgctg tcaatagcgt catcccaata atgtaacggc gcaagccttt ggtgcaggcc
     1141 cagatgaaga gtaaaaagcc acgcggcggt ggcttggtca ttgtgtcagg ataggggtaa
     1201 accagttttt caaaccatgc aagcatgtaa tctccagagt attggcagcc tgttgtgcct
     1261 cacttgctta tttcgattcg tagccgaaaa ctcgcagatt atttattatc gcagacagct
     1321 gcatgcggaa tttatctgga taagtttcat gaccgcgaaa aagtggcggc cctatcacac
     1381 agtaaagtat gcgcaagtcc atgaatgcat ggcgtaaaat aagctgtttc ctgattgaag
     1441 gcttcgtcca tcatgcctct tcccgacaaa gaatccctga tcgaatatcc aagcgacttt
     1501 cccatcaaag tcatcgccat catgcacgat gaattcgtca atcatatcgt cgaaatgatt
     1561 actcagcatg atcccacttt tcatgccggc aaggtcgaga tacgtccttc ttcgcaaggc
     1621 aaatacctgt cgctgaccgt gaccgtactc gctaccagcc gcgcacaact cgacaatctg
     1681 tatcgtgcct tgtcctcgca tgagatggtg aaattcgtgc tataaatcgc catgcaggcc
     1741 gttccacaaa tcatccggcg cggcattgaa gattatcagg ctagcttcga tgccatgcgc
     1801 gcctacaccg ataaccgaac tcccgatacc cacgaccaac tctggatcgt cgaacatccg
     1861 tcggtgttca cactcggcct gggcgcagac atcgcccata tacttgatcc ccacgccatt
     1921 cctgtaatcc agaccgaccg tggcggcgaa gtcacttacc atggccccgg ccaggtggtg
     1981 atctatctgc tgatggattt gcgccgtcat cattcaggtg cgcgtctact cgtccgtgaa
     2041 ttcgtgcaaa aaatcgaaca ggcggtgatc gacacactag caacgtataa tcttgctggt
     2101 gaacgtaaac aagggacgcc cggcatctat gtcagcacag gacgatggca aggcgccaag
     2161 atcgctgctc tgggtttgaa aatccgtagc aacgcttgca cctatcacgg cgtatcgctc
     2221 aacgtggcga tggatctgac accgttttcc tggatcaacc cttgtggcca tgcaggtctc
     2281 gccaccgttg acatgcgaac actgggcgcg gctgccgagc caagtgaggt acaaacgttg
     2341 cttaccgatc gactcgtgga gtatttcgtt ttaccgccgt ctgcctgaga tggcttcaaa
     2401 tatcatgatg atgactacta acattgctcc aaccaaagct ttgcgcaatc ctgccgacaa
     2461 acaaaagggc gctggcaaaa ctgcctggat tcccatcaag gtcatgccgg cggaaaaact
     2521 caaaaagccc gactggattc gcgtcaaagc cggctcgccg accacccgct tctatgaaat
     2581 caaagacatc ctgcgtgcca gcaatctggt gacggtatgc gaagaagctt cctgccctaa
     2641 tatcggcgaa tgcttcggta aaggcactgc cacctttatg atcatgggtg acaaatgcac
     2701 gcgccgttgc ccgttctgcg acgttggcca tggccggccc gatccacttg atcccaatga
     2761 accggaaaat cttgctagga ccatcgctca gctcaagctg agctacgtcg tcatcaccag
     2821 cgtcgatcgc gacgatctgc gcgacggcgg cgcaacccat tttactgact gcatccagcg
     2881 tgtgcgcgaa ctctcgccca cgactcgtat cgaaatcctg acaccagatt tccgtggacg
     2941 catagatcgc gcactggaaa ttttcaagac agccccgcca gacgtcatga atcataacct
     3001 tgaaactgca ccacgcctct acaaagaagc gcgtcccggt tccgattacg aatattcgct
     3061 gaatctgctg aaacgtttca aagaactgca tccggatacg ccaaccaaat ccggcatcat
     3121 ggttggcctc ggtgaaactg atgaagaagt cctgcaagtc atgcgcgaca tgcgcgccca
     3181 tgacgtcgac atgctcacta tcggccaata cctgatgcca agcaacgacc atctgccggt
     3241 acgccgttat gtacacccag acaccttcaa aatgtatgaa gaagaagcct gcaagatggg
     3301 gttcatgcat gcagctgtag gggcaatggt gcgttcaagc taccatgccg accagcaggc
     3361 gcatggcctg gttggctaac tgtactccaa gttcatctgt gaacaaatcc ttgcatatct
     3421 cccatatgaa atgtgaatag ccctgcaatt cttcacaggg ctattttttt attcagtttc
     3481 tgtttttttg ccatacaaga cttactaaac acttatgcat ttcacaaatc cttgatctaa
     3541 tactggttta cttggttatg cacagaaaaa gtgtgttgtt aactattatt actattttta
     3601 tatagaacat ttaattaatt aaaaatgaac tgctatgaac cttgtaggtt gaaaaaatat
     3661 ctgtaaaggg tagttatgaa ggtgggagtt aaaagtagtt gacgtagatg aaagagtggt
     3721 gcataatctt gtttttgctc aattgccaga cagaacgatt tggtggttga gcgggcagtg
     3781 gagtggaagt agctgcgt
//
LOCUS       DASRDH010000290         4895 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1215157,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000290 DASRDH010000000
VERSION     DASRDH010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 4895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4895
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1215157"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..110
                     /locus_tag="VFA14_06750"
     CDS             <1..110
                     /locus_tag="VFA14_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019922805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HZS81106.1"
                     /translation="LSELNAIVKAARCRFPQQAFRNASLFAQLGIHADR"
     gene            136..339
                     /gene="rpoZ"
                     /locus_tag="VFA14_06755"
     CDS             136..339
                     /gene="rpoZ"
                     /locus_tag="VFA14_06755"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HZS81107.1"
                     /translation="MARITIEDCLKQIPNRFYLTLAATYRARQLLQGHTPKVEAKNKP
                     TVTALREIAAGKVGIEMLKKVPS"
     gene            375..2633
                     /locus_tag="VFA14_06760"
     CDS             375..2633
                     /locus_tag="VFA14_06760"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008328297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HZS81108.1"
                     /translation="MSLIPTDSSPDSPSSAQPGTARASSACPAPKPSTARNGVATFAH
                     LTHELKAYLTPSELKKVQKAYRFSDEVHLGQMRKSGEPYISHPLAVAEICAEWKLDAQ
                     AMMAALLHDVMEDQGVRKEELIERFGAKVAALVDGLSKLDKLEFQSQAEAQAENFRKM
                     LLAMARDVRVILVKLADRLHNMRTLGSMPPDKKRRISRETMEVYVPIAHRLGLNNVYR
                     ELQELSFSYLYPLRHRTLLKAVKAARGNRREVVTKILESVTSTLIAAGIPAQIDGREK
                     TLFGIYRKMRNKHLSFSQVLDVYGFRVVADSLANCYVALGTLHSLFKPMPGKFKDYIA
                     IPKLNGYQSLHTTLIGPYGTPVEFQIRTQDMHHVAESGVAAHWLYKNGEGSLTDLQQR
                     THIWLQSLLDIQKQTGDSIEFLENIKVDLFPDSVYVFTPKSRIIALPRGATALDFAYS
                     IHTDIGNQTTSAVINHEPVPLRTELHNGDIVEIITSPSSHPSPNWLSYVRTGRARSAI
                     RHHLRNVNLAESQALGKRLLTNALNTLGLEQDLPSALIERLLNESSAKTMEDVYADIG
                     VGTRMAPLVARHIMGMIEGSSAIPQLDVEGQILPGRPEPVIITGNEGSIVQLAPCCLP
                     IPGDRLIGNMKHDQALIVHTEDCETAGRYREKEPERWIDVTWGRELNRRFECRIAIVA
                     HNKKGVLARIAAEIGEADAHIVLVSMDEADSPSMKHLRFTIQVEDRVHLARTMRGIHS
                     IDGVARILRERS"
     gene            complement(2648..3982)
                     /locus_tag="VFA14_06765"
     CDS             complement(2648..3982)
                     /locus_tag="VFA14_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007881833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3422 domain-containing protein"
                     /protein_id="HZS81109.1"
                     /translation="MVYTSLNHPQRVELAAEVHSRPFLRLEAPETLTHFAVYVQTDAS
                     FHAAVQHDLLIDLCDRFGVTAPDINAKHFFHDFGRFRLKWECHTEFSTYTFAQRHETA
                     LSTEEAFAAVPLTQIPQDWLQTLQGKIIMAAHVVLERASAGQSGESIQSMQRVFEGKR
                     LVSSKVVQGGEIWTDFLIQSDGFSRFVVRDIGLRDMQGGQLVQRILDIETYRMMALLG
                     LPYAQRAAPLLSAIENDLVMLTATVVDADHGVGNPLLAQAEDELNLSKITSLATRIEK
                     TSLENSYRFSASQAYFRLVKARIEELRESRMEEGVPTIGEFMERRLTPAMDTCISVAR
                     RQETLADRIAHTNDLLRTRVGIVQEQQNRQILESMNTRAEQQLRLQQAVEGLSVAAIS
                     YYMVGLFGYAGKALKAVGLPVDPDILTGIMLPVIAVGVWLGLRRMHKSVHHS"
     gene            complement(4007..4561)
                     /gene="greB"
                     /locus_tag="VFA14_06770"
     CDS             complement(4007..4561)
                     /gene="greB"
                     /locus_tag="VFA14_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HZS81110.1"
                     /translation="MSKAFVKESDDDDDGLVLPAIPDGVRNYMTPQGHKRIKDELLHL
                     IDVARPETVRIVHWAASNGDRSENGDYIYGKRRLREIDRRIRFLTKRFDIAEVVDPAR
                     HYGNDQVFFGATVTYANQDGVAHTVTIVGIDELDPLNGKISWISPVARTLTKAREGDE
                     VSLQTPAGVERLTILKVCYPAPQS"
     gene            complement(4629..>4895)
                     /gene="rlmJ"
                     /locus_tag="VFA14_06775"
     CDS             complement(4629..>4895)
                     /gene="rlmJ"
                     /locus_tag="VFA14_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HZS81111.1"
                     /translation="AVWYPVLQRIESRQFSHRLKRLGARSWLNVTLSVCSPSPDGFGL
                     HTSGMFVLNPPWTLEATLRGVMPYLVKVLGRDSGAGFVLESGEV"
BASE COUNT         1118 a         1509 c         1297 g          971 t
ORIGIN      
        1 ccttgtcaga attgaatgcc attgtgaaag ctgcccgttg ccgctttcca caacaagcct
       61 tccgcaacgc ctccttgttc gctcagcttg gtattcatgc tgatcggtag caagtcatct
      121 catcaggaat tatttatggc ccgtatcacg attgaagatt gtctcaagca aatccctaac
      181 cgtttttatc tgaccttggc cgcgacgtat cgtgcgcgtc agctgctgca gggccacacc
      241 ccgaaagtgg aagccaagaa caagccgacc gtaaccgcgc tgcgcgaaat cgccgctggc
      301 aaggtcggta tcgagatgct gaaaaaagtg ccttcctgat ccattctcgt tcatgccatc
      361 gatgacgctg acgtatgagc ctgataccaa cagattcaag cccagattca ccgtcgtcag
      421 cgcagcctgg caccgcccgg gcatcttccg cgtgcccagc accgaaaccg tccactgcgc
      481 gcaacggcgt ggcaacattt gcccatctga cgcacgagct caaggcatac ctcacgccat
      541 ccgagctgaa aaaggtccag aaagcctatc gcttctcgga cgaagtgcat ctcggccaga
      601 tgcgcaaatc gggcgaaccg tatatctcac atccgctggc agtcgccgaa atctgcgccg
      661 aatggaagct cgacgcgcag gccatgatgg cggcgctgct gcacgacgtg atggaagatc
      721 agggtgtcag gaaggaagaa ctaatcgaac gcttcggcgc gaaggtcgct gcgctggtcg
      781 acggactgtc caagctggac aagctggagt tccagagcca ggccgaagcc caggctgaaa
      841 atttccgtaa gatgctgctg gcgatggcgc gcgatgtgcg cgtgatcctg gtcaagctgg
      901 ccgaccgcct gcacaatatg cgcacgctgg gttcgatgcc accagacaaa aaacgccgca
      961 tctcccgcga gaccatggaa gtctatgtac cgatcgctca tcgcctcggc ctcaacaatg
     1021 tttaccgtga gttacaggag ctgtcgttct cgtatctcta tccattgcgt catcgcacac
     1081 tgctcaaggc agtcaaggcg gcgcgcggca atcgccgcga ggtcgtgacc aaaattctcg
     1141 aatccgtcac cagcacgctg atcgctgctg gcatcccggc acaaatcgac ggccgcgaaa
     1201 aaaccctgtt cggcatctac cgcaaaatgc gcaacaagca tctgtcgttc tcgcaggtgc
     1261 tggacgtcta cggcttccgc gtggtggccg acagcctggc caattgctac gttgcactgg
     1321 gtacgctaca ttcgctgttc aagccgatgc cgggcaagtt caaggattac attgcgattc
     1381 ccaagctcaa cggctatcaa tcgctgcata ccacactgat cggcccctac ggcacaccgg
     1441 tggagttcca gatccggacc caggacatgc atcacgtcgc cgaatcgggc gtagcggcgc
     1501 actggctata caagaacggc gaaggcagct tgaccgacct gcagcagcgc acgcatatct
     1561 ggctgcaatc gctgctcgat attcagaaac aaaccggcga ttcgatcgag ttcctcgaga
     1621 acatcaaagt tgacctgttc ccggattccg tctacgtatt caccccgaag tccaggatca
     1681 ttgccctgcc gcgcggcgcc acggcgctgg attttgccta tagtatccac accgacatcg
     1741 gcaaccagac cacttcggca gtgatcaacc acgaaccagt gccactgcgt accgaactgc
     1801 acaatggcga catcgtcgaa atcatcacct cgccatcctc gcatccaagt ccgaactggc
     1861 tcagctatgt gcgcaccggc agggcgcgct cggccatccg ccatcacttg cgcaacgtca
     1921 acctggccga atcgcaagca ctgggcaagc gcctgttgac caatgcgctc aacacattgg
     1981 gcctggaaca ggatctgcca agcgcattga tcgagcgtct gctcaacgaa tccagcgcca
     2041 agaccatgga agatgtgtac gccgacatcg gcgtcggtac acgcatggcg ccgctggtgg
     2101 cacgtcacat catgggcatg atcgaaggca gcagcgcgat cccgcagctc gatgtcgaag
     2161 gccagatact gccgggcagg ccagagcctg tcatcatcac cggcaacgaa ggctcgatcg
     2221 tgcagctggc tccctgctgt ctgcctatcc ccggcgacag gctcatcggc aacatgaagc
     2281 acgaccaggc tctgatcgtc cacacggaag attgcgaaac agccggtcgg tatcgtgaga
     2341 aagaaccgga gcgctggatc gatgtcacct ggggcagaga gttgaaccgc cgcttcgaat
     2401 gccgcatcgc catcgtggcg cacaataaaa aaggcgtgct ggcgcgtatc gcagctgaaa
     2461 tcggcgaagc cgacgcgcat atcgtgctag tcagcatgga tgaggcggac tccccatcga
     2521 tgaaacacct gcgcttcacc atccaggtcg aagaccgcgt tcacctggcg cgcaccatgc
     2581 gcggcatcca cagtatcgac ggtgtcgccc gcatcctgcg tgagcgtagc taggtgttca
     2641 gggccgctca ggaatgatgc acgctcttgt gcatcctgcg cagtcccagc cagacaccga
     2701 cagcgatcac cggcagcatg atgccggtga ggatatccgg gtcgaccggc aatcccacgg
     2761 ccttcaaggc tttgccggca taaccgaaca aacccaccat ataataggaa attgccgcca
     2821 ccgacaatcc ttcgacagcc tgctgcaaac gcaactgctg ctcggcacgc gtattcatcg
     2881 actccaatat ctggcgattc tgctgttcct gcacgatgcc cacgcgcgtg cgcagcaaat
     2941 cattggtatg agcgatgcga tcagccagcg tttcctgacg gcgcgctacc gaaatgcagg
     3001 tatccatcgc cggcgtcagc cgtcgctcca tgaactcgcc aatggttggc acgccttctt
     3061 ccatacggct ttcgcgcaac tcttcgatgc gcgccttcac cagacggaaa tacgcctgcg
     3121 aggcagagaa gcgataactg ttttccagcg atgtcttttc aatgcgtgtc gccaggctgg
     3181 tgattttgct caggttcagt tcatcctcgg cctgcgccag taacggattg ccgacaccat
     3241 gatcagcgtc gaccacagtc gcagtcaaca tcaccagatc gttttcgatg gcgctcaaga
     3301 gcggcgctgc ccgctgcgca tacggcagac cgagcaaggc catcatgcgg taagtttcaa
     3361 tatccaggat acgctgcacc agctggccgc cctgcatgtc gcgcaaaccg atatcacgca
     3421 ccacaaagcg actgaaccca tcggactgaa tcagaaaatc ggtccagatt tcaccaccct
     3481 gcactacctt gctcgacacc aagcgtttgc cttcgaacac acgctgcatg gactggatgc
     3541 tttcaccgct ctggccggcg ctcgcgcgtt ccaggacgac gtgcgctgcc attatgatct
     3601 tgccctgcaa tgtttgcagc cagtcctgcg gtatctgagt gagcggtact gcggcaaaag
     3661 cctcttcagt actcagcgcc gtctcatgac gctgcgcgaa ggtataagtg gagaactccg
     3721 tatggcattc ccatttcagg cgaaaacgtc cgaaatcatg aaaaaaatgc ttagcgttga
     3781 tgtcaggcgc ggtcacgccg aaacggtcgc acaggtcgat cagcaagtca tgctggacgg
     3841 ctgcatggaa gctggcatcg gtttgcacgt acacggcaaa atgcgtgagc gtctcaggtg
     3901 cctccagacg caaaaaaggc cgcgaatgga cctccgccgc caattcgacg cgctggggat
     3961 gattcaagct agtatacacc ataaacttcc cttttcaaat caaaaatcag gattgcggcg
     4021 ccggataaca gaccttgaga atggtcagtc gctccacccc tgccggcgtc tgcaagctca
     4081 cttcgtcacc ttcgcgcgcc ttggtcaggg tacgcgccac cggcgaaatc cagctgatct
     4141 tgccattcaa tggatcaagt tcatcgatac caacgatggt gacagtatgc gcgacgccgt
     4201 cctggtttgc atacgtgacc gtcgcgccaa aaaaaacctg atcgttgcca taatgcctgg
     4261 ctggatcgac cacctcggca atgtcgaagc gtttggtcag aaaacggatg cgccggtcga
     4321 tctcgcgcag gcggcgctta ccgtagatgt agtcaccgtt ttccgaacgg tcgccattgg
     4381 aggcggccca atgcacgatg cgtacggttt ccggacgagc gacgtcgatc agatgcagca
     4441 gttcatcctt gatgcgcttg tgtccttgcg gcgtcatata atttctgacg ccgtccggta
     4501 ttgccggcaa tacaaggccg tcgtcatcat catcggattc tttcacaaat gctttactca
     4561 tcgtcattgt agcgatatgt cgagttccca aacatgcgct ttacgagtaa cagatactca
     4621 tcacggtcct agacttcccc cgactccagc acaaaaccgg cgccgctgtc gcgccccagt
     4681 accttgacca aataaggcat gacgcctctg agcgtcgctt cgagcgtcca tggagggttg
     4741 agtacgaaca tgccactggt atgcaggcca aaaccatcgg gcgatggcga acagaccgac
     4801 agcgtcacat tcagccagct cctggctccc aggcgcttga ggcgatggga aaactgtctg
     4861 gactcgatgc gttgcagtac aggataccag accgc
//
LOCUS       DASRDH010000291         2143 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_420647,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000291 DASRDH010000000
VERSION     DASRDH010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420647"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..721
                     /gene="secD"
                     /locus_tag="VFA14_06780"
     CDS             <1..721
                     /gene="secD"
                     /locus_tag="VFA14_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007877764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HZS81112.1"
                     /translation="TVATIRSELGSRFQITGMGSAEAANDLALLLRAGSLAAPMEIIE
                     ERTIGPQLGAENIRKGFDATMYGFAAMAVFMIVYYQLFGLFSALALSVNVLLLIAVLS
                     TLQATLTLPGIAAIALALGMAIDSNVLINERIREELRNGNSPQAAIAAGFERAWATIL
                     DSNVTTLIAGLALLIFGSGAIRGFAVVHCLGILTSMFSAVFVSRGIVNLWYGRRKKLI
                     GLAVGQIWKPQARIDAKAGVK"
     gene            736..1680
                     /gene="secF"
                     /locus_tag="VFA14_06785"
     CDS             736..1680
                     /gene="secF"
                     /locus_tag="VFA14_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007877763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HZS81113.1"
                     /translation="MEFFRIKKDIPFMRHALMFNIISALSFVLAVFFLFSRGLHLSIE
                     FTGGTVMELAYTRPANIENIRKSVEDLGFTDAQVQSFGTAQDVMIRLPAQHGVNSTQQ
                     SGKVIEALTAQDPDVKLQRVEFVGPQVGDELAHDGAMALGMVVLGIVIYLAFRFEWKF
                     AVAAVVANLHDVVIILGFFAFFQWEFSLTVLAAVLAVLGYSVNESVVVFDRVRETFRD
                     RRYGNLSVPEILNHAITSTISRTIITHGSTEMMVLSMLIFGGQTLHYFALALTIGILF
                     GIYSSVFVAASIAMWLGVRREDLLKPVKPKDKTDGAVV"
     gene            1706..>2143
                     /locus_tag="VFA14_06790"
     CDS             1706..>2143
                     /locus_tag="VFA14_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HZS81114.1"
                     /translation="MTVMSDIALNETGIDFDRRFGGIARLYGAQGLARLRAAHVCVVG
                     VGGVGSWVVEALARSAIGKITMIDLDNLAESNINRQIHALSATLGQAKVTALHERIMQ
                     INPYCEVTSIEDFLTPDNVGEMMGIERFDYVIDAIDDVRAKTAL"
BASE COUNT          399 a          552 c          653 g          539 t
ORIGIN      
        1 gaccgtggcg actatccgtt ccgagcttgg ctcgcgtttc cagatcaccg gtatgggttc
       61 agccgaagcc gccaacgatc tggcgctgct gctgcgcgcc ggttccctgg ccgcaccgat
      121 ggaaatcatc gaagaacgca ccatcggtcc gcaactcggc gcggagaaca ttcgcaaggg
      181 ctttgatgcg acgatgtacg gctttgccgc catggccgtg ttcatgatcg tgtattacca
      241 gttgttcggc ttgttcagtg cgctggcgtt gtcggtcaat gtgttgctgc tgattgcggt
      301 gttgtcgacc ttgcaggcta cgctgacttt gcccggtatc gctgcgatcg cgctggccct
      361 cggtatggcg atcgactcaa atgtgttgat caacgagcgt atccgcgaag agctgcgtaa
      421 cggtaattcg ccgcaggcag cgattgcggc cggcttcgag cgcgcttggg cgaccatcct
      481 ggattctaat gtgaccacgc tgattgccgg tctggcgctg ttgattttcg gttccggcgc
      541 gatccgcggt tttgcagtgg tgcattgtct gggcatcttg acgtcgatgt tttcggccgt
      601 gttcgtttcg cgcggtatcg tcaatctgtg gtacggccgc aggaaaaagc tgatcggcct
      661 ggccgttggc cagatctgga agccgcaggc caggatcgat gcgaaagcgg gcgtcaaata
      721 acggaaaggt atgtgatgga attttttcgc atcaaaaaag atatcccctt catgcgccat
      781 gcgttgatgt tcaacattat ctcggcgctg agtttcgtgc tggcagtatt ctttctgttc
      841 agtcgcggtt tgcacttgtc gatcgaattc accggcggta cggtgatgga actggcttat
      901 accaggccgg ccaatatcga gaacatccgc aagtcggtgg aggatctggg cttcactgat
      961 gcgcaggtac aaagttttgg tacggcgcag gacgtgatga tccgtctgcc ggcgcagcat
     1021 ggtgtcaatt cgacgcagca aagcgggaaa gtgatcgagg cgctgacggc gcaggatcct
     1081 gatgtcaaac tgcagcgcgt cgaattcgtc ggtccgcagg tcggtgacga actggcgcat
     1141 gatggcgcga tggcactggg catggtggta ctcggcatcg tgatctatct ggccttccgt
     1201 ttcgagtgga aattcgctgt cgccgctgtc gtcgccaacc tgcacgatgt ggtgatcatc
     1261 ctcggtttct tcgccttctt ccaatgggaa ttttcactga cggtgctggc ggcggtactg
     1321 gcggtgctgg gctattcggt caatgagtcg gtggtggtgt tcgaccgggt gcgtgaaact
     1381 ttccgcgacc gtcgttacgg taatctgtcc gttcctgaaa tattgaatca tgcgattacc
     1441 agtaccatct cgcgcaccat catcactcac ggctccacgg aaatgatggt cttgtcgatg
     1501 ttgatcttcg gtggccagac gctgcattat tttgccttgg cgctgaccat cggcatttta
     1561 ttcggcattt attcgtcggt gttcgtcgct gcctccatcg ccatgtggct gggcgtcaga
     1621 cgcgaagatc tgctcaagcc ggtaaagccg aaggataaaa cagacggcgc ggtggtctag
     1681 gtttgccgtg tgtaaaaccg gcactatgac ggttatgtcc gatattgcat tgaatgaaac
     1741 cgggattgat ttcgaccgcc gtttcggtgg catcgcccgt ctgtatggcg cgcaagggct
     1801 tgcgcgtttg cgtgcggcgc atgtttgcgt agtcggcgtc ggcggagtcg gttcatgggt
     1861 cgtcgaagcg ctggcgcgca gcgccattgg caaaatcacc atgattgatc tcgacaatct
     1921 ggccgaatcc aacatcaatc gtcagatcca cgcgttgagc gctacgcttg gtcaggccaa
     1981 ggtaacggcg ttgcatgagc gcatcatgca aatcaatccg tattgcgaag tgaccagcat
     2041 cgaggatttt ctcacgcctg acaatgttgg cgagatgatg ggcatagagc gcttcgatta
     2101 cgtcatcgac gctatcgacg atgtgcgtgc caaaacggcc ttg
//
LOCUS       DASRDH010000292         5002 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_515341,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000292 DASRDH010000000
VERSION     DASRDH010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 5002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5002
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_515341"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1409)
                     /locus_tag="VFA14_06795"
     CDS             complement(<1..1409)
                     /locus_tag="VFA14_06795"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008330270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HZS81115.1"
                     /translation="MYRYDQYDHLIVKERVAQYRDQVCRRLSDELSEDKFRILRLQNG
                     LYLQRHAYMLRVAIPYGLLASRQVRMFAHIARKYDRGYGHFTTRQNIQFNWIRLGDSP
                     DILAELATVEMHAIQTSGNCVRNTTSDELAGVAADEIADPRPYAELIREWSTFHPEFA
                     YLPRKFKIAISGSAQDRAATAVHDVGLHVIKNDAGETGFRVLVGGGMGRTPILGSVIR
                     DFLPWQHAMSYLEAILRVYNQYGRRDNIYKARIKILVKAIGPEEFARQVEEEWRHIKD
                     GPSTLTTEELQRVASHFTAPAYETLPAIDAGYQRLQTEDKGFASWIKRNVKQHKVPGY
                     AAVILSLKKPGLPPGDITADQMEFVADLADRYSFGELRVTHEQNLTLADVKQSALYTL
                     WQEAKEHGLATPNIGLLTDIICCPGGDFCSLANAKSIPIAQAIAERFENIDFQHDIGD
                     IELNISGCINACGHHHVGN"
     gene            complement(1506..2279)
                     /locus_tag="VFA14_06800"
     CDS             complement(1506..2279)
                     /locus_tag="VFA14_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006465658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HZS81116.1"
                     /translation="MTLSYVVSGFTVGLLVGLTGVGGGALMTPILTLLFGIPPSVAVG
                     TDLAFAAATKTAGTFAHRFKGTVRWDIVRRLSYGTLPAALITTLLLKHFGAISADIST
                     VIRYCIAVSVFLTVIALLFRGKLQAWFNAHPERQLQGHRLANTTILTGAVIGTLVTVS
                     SIGAGVIGATLLVLLYPRLSPAEIAGTDIAYAVPLTAIAACGHWWLGSIDWSLLGALL
                     LGSVPGITIGSFAARAAPEKFLRGLLAIALTSVAFKLIY"
     gene            2408..3349
                     /locus_tag="VFA14_06805"
     CDS             2408..3349
                     /locus_tag="VFA14_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006398571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysB family HTH-type transcriptional regulator"
                     /protein_id="HZS81117.1"
                     /translation="MNLHQFRFVREAVRQNYNLTEAAKALYTSQPGVSKAIIELEEEL
                     GVDIFARHGKRIRGLTEPGRAVLRLVESIMQEVDGLKRIGKEFAAHDSGNLTIATTHT
                     QARYSLPRVVQTFMQRYPKVRLSLLQGNPRQIVEMVRNDQADIAIATEALASGEGLLS
                     LPCYQWEHVVVVALDHPLLQSRNLSLEEIAGYPLITYDAAFSGRSKIDHAFALRDLKP
                     DIVLEAIDADVIKTYVELGMGVGIIAGMAFDAGRDCGLRALPAGHLFGSNVSRVALKQ
                     GAYLRSYVYTFIELLAPTLNRKLIAQVMEGGKERYEL"
     gene            3496..3954
                     /locus_tag="VFA14_06810"
     CDS             3496..3954
                     /locus_tag="VFA14_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013233815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HZS81118.1"
                     /translation="MSIYEKLYALNIALPAVAVPVAAYVMYAQASNTVFLSGHLAKKD
                     GKVWVGQFGRNITTEEGRLAARATAIDLLATLQAACGGDLTRVKRIVKLMSLVNSSGD
                     FTEQHLVTNGASELICELFGEQGRHARSAFGVAQLPMGACVEIELIAEIA"
     gene            4317..4763
                     /locus_tag="VFA14_06815"
     CDS             4317..4763
                     /locus_tag="VFA14_06815"
                     /inference="COORDINATES: protein motif:HMM:NF013786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HZS81119.1"
                     /translation="MAAIVALDRVVGIDVERRDRPLDLDIAALICSPAEVAALHAAPR
                     SRQREKLLQLWVRKEALTKAVGRGLSMALPLIELDDDGFTVSGQSVPGMEQPADWQVH
                     LLTMVPNVLLAMAVHCQGQHLCIRHTRIDWVRLCEFCGIATECVNV"
     gene            complement(4753..>5002)
                     /locus_tag="VFA14_06820"
     CDS             complement(4753..>5002)
                     /locus_tag="VFA14_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HZS81120.1"
                     /translation="LKTLTNGGRGVILMELEKNEKLLAAQAITQQGVLVSGTGRGGRV
                     QEVALSVTALSLHIGKRARKGRALQSKLKATALAPVIR"
BASE COUNT         1012 a         1379 c         1524 g         1087 t
ORIGIN      
        1 atgttgccga catggtgatg gccacaagcg ttgatgcagc ctgagatgtt cagttcgata
       61 tcgccgatgt cgtgctggaa gtcgatattc tcgaagcgct cggcaatcgc ctgcgcaatc
      121 gggatcgact tggcattcgc cagcgagcag aagtcgccgc cggggcagca gatgatgtcg
      181 gtcagcagac cgatgttggg tgttgccaga ccgtgttctt tagcctcttg ccatagcgtg
      241 tacagtgcgc tttgcttgac atcggccagc gtcaggttct gctcgtgtgt gacgcgcagt
      301 tcgccaaagc tgtaacggtc ggccaggtcg gcgacgaact ccatctggtc ggcagtgatg
      361 tcgcccggcg gcaggccagg ttttttcagc gacaggatca cggcggcata gccgggtact
      421 ttgtgctgtt tgacgttgcg tttgatccag ctggcgaagc ccttgtcttc tgtttgcaga
      481 cgctggtagc cggcatcgat ggcgggcagg gtttcatacg ccggcgcggt gaaatggctc
      541 gccacgcgtt gcagttcttc tgtggtcagc gtcgatgggc catccttgat atgtcgccat
      601 tcttcttcga cctgacgggc gaattcttca gggccgatgg ccttgaccag aatcttgatg
      661 cgtgccttgt agatgttgtc gcgacgaccg tactggttgt acacgcgcag aatcgcttca
      721 aggtatgaca tggcgtgttg ccatggcagg aagtcacgga tcacgctgcc gaggataggc
      781 gtgcggccca tgccgccgcc gaccagcacg cggaaaccgg tttcgccggc gtcgtttttg
      841 atgacgtgca gaccgacgtc atgcacggcc gtcgcagcgc ggtcctgcgc ggaaccgctg
      901 atggcgatct tgaacttgcg cggcagataa gcgaactcgg ggtggaaggt gctccactcg
      961 cggatcagtt cggcgtaagg acgtggatcg gcgatctcgt cggcggcaac gccggccagt
     1021 tcgtccgagg tggtgttgcg gacgcagttg ccggaggtct ggatggcatg catttcgacc
     1081 gtggcgagtt cagccagaat atcgggagag tctcccagcc tgatccagtt gaactggatg
     1141 ttctggcgcg tggtgaagtg gccgtagccg cggtcgtatt tgcgcgcgat gtgagcaaac
     1201 atgcgaacct gacgcgatgc cagcaggcca taaggaatcg cgacgcgcag catgtaggca
     1261 tggcgttgca gatacaggcc gttttgcagg cgcaggatgc ggaatttatc ttcgctcagt
     1321 tcatcggaga gacggcggca cacttggtct cgatattgcg caacccgttc tttgacgatg
     1381 agatgatcgt attggtcgta gcggtacatc ctgtagtctt tgttaacgtt ctgtcggagt
     1441 cctgcggtcg agactccgtg tgattaaaaa aactgaaacc aagagccact ctagcagagc
     1501 ggctcttagt aaatcagctt gaaagcgacg cttgtcagcg caatggccag caggccacgc
     1561 aagaattttt ccggcgccgc gcgcgctgcg aacgagccga tggtgatgcc gggcaccgaa
     1621 cccagcaata atgcgccgag caggctccaa tcgatcgaac ccagccacca gtggccgcat
     1681 gccgcgatgg ccgtcaacgg caccgcataa gcaatatcgg tgccggcgat ttccgccggc
     1741 gacaggcgcg gatacagtag caccagcaaa gtcgcgccga tgacgccagc gccgatcgaa
     1801 gaaaccgtca ccagcgtgcc gatcacggcg cctgtcagga tggtggtatt ggccagccga
     1861 tggccttgca gctggcgttc cggatgggca ttgaaccaag cctggagttt acccctgaac
     1921 agcaaggcga tcacggtaag aaaaacggag acggcaatgc aatagcggat gacggtactg
     1981 atgtctgcgc tgatggcgcc gaagtgtttc aacagcaacg tggtgatgag ggcggcaggc
     2041 agcgtgccgt aggacaggcg gcgcacgatg tcccagcgca cggtgccctt gaagcgatgc
     2101 gcaaaggtgc cggccgtttt cgtcgccgca gcgaaagcca gatccgtgcc aactgctacg
     2161 ctggggggga tgccgaacag caaggtcagt ataggcgtca tcagtgcgcc gccgccgacg
     2221 ccggtcagtc cgaccagcaa tcccaccgta aaaccgctga ctacataaga aagagtcatg
     2281 acacctcatc tataacagac tgaatcgtaa taaacttggg tgccaaaccc aactacatac
     2341 tatttatttg cttatatgca caattggcat gagcaaatag agttgccggg actaatgaag
     2401 gacagtcatg aatttgcatc aattccgttt cgttagggag gcggtaaggc aaaactacaa
     2461 cctcaccgaa gccgccaagg cactatatac ctcgcagccg ggtgtctcga aggccatcat
     2521 cgagctggaa gaagaactcg gcgtagacat cttcgcgcgt cacggcaagc gcatccgtgg
     2581 cctgaccgaa ccgggccggg cggtgctgcg tctggttgag tcgatcatgc aggaagtgga
     2641 tggcctgaag cgtatcggca aggaattcgc cgcccatgac agcggcaatt tgaccatcgc
     2701 caccacgcat acccaggcgc gctattcctt gcccagggtg gtgcagactt tcatgcaaag
     2761 atatcccaag gtcaggttat ccctgctgca aggcaatccc aggcagattg tcgagatggt
     2821 gcgtaacgat caggccgaca tcgccattgc cactgaagca ctggcctcgg gcgaggggtt
     2881 gctctctttg ccgtgttatc aatgggaaca cgtggtggtg gtcgcgctcg atcatccttt
     2941 gctgcaatcc aggaacttgt cgttggaaga aatcgccggc tatccgttga tcacctatga
     3001 cgccgccttc agtggccgca gcaagatcga tcacgccttt gcgttacgtg acctgaaacc
     3061 ggacattgtg ctcgaagcca tcgacgccga tgtcatcaag acctatgtcg agcttggtat
     3121 gggcgtgggc atcatcgccg gcatggcttt tgatgccgga cgcgactgcg gtttacgcgc
     3181 gctgccggcc ggtcatctgt tcggcagcaa tgtctcgcgt gtggccttga agcagggcgc
     3241 ctatctacgc agctatgtgt atactttcat cgaactgctt gcgccgacgc tcaatcgcaa
     3301 actgattgcg caggtcatgg agggcgggaa ggaacgatac gaactttgag ccgtggagca
     3361 gggcggacca gctcagagct gcgctgtttg cgataatgcc aggcttcatt gcttttcccg
     3421 ttttttattt cacaacctgc catcttgccg ggcagtgctg ctcggcaagg tagtcatcga
     3481 acacaggagt agtacatgtc catctacgaa aaactctatg ccctgaatat tgccttgcct
     3541 gccgtcgcag ttcccgtggc cgcttatgtc atgtacgcgc aggcaagcaa cacggtattt
     3601 ttatccggcc atctggccaa aaaggatggc aaggtctggg ttggccagtt cggcaggaac
     3661 atcacgacgg aagagggcag gctggctgcg cgtgcgacgg cgatcgattt gctggcgaca
     3721 ttgcaggcgg cttgcggtgg cgatctgact cgcgtcaagc gcatcgtcaa gctgatgagc
     3781 ctggtcaatt cgagcggcga ttttactgag cagcacctgg tcaccaatgg tgcgtcggaa
     3841 ctgatctgtg aactgttcgg cgagcaaggc aggcatgcgc gttcggcatt tggtgtggcg
     3901 cagctgccga tgggtgcttg cgtcgagatc gaactgatcg ccgaaatcgc ttgagaacgt
     3961 gttcccgatc tcgtcgctag ttgttcggtc accttctttt cagcaagcag tctatgcatc
     4021 ctgacgcagt tcgcatctgg agcatggtga cgcatgaggt gcctgaatcg acgctgcaac
     4081 ggttctctca ggtactggca ccatgggaac gatcccgcgc agaaagatac ctcagcgcgc
     4141 tggatcgtag agactatctt tgcgcacacg tgctgcgcag gtcgctgctt tctcactgtt
     4201 ttcatcgatc catcagtgac tgggtctttg aggcagattc cttcggcaag ccgactgttc
     4261 tcaatcgaga gccatgtggc cgaatcgaat gcaatctctc acatacgcga ggcgtcgtgg
     4321 cggcgatcgt cgcgctcgac cgagtcgtcg gcatcgatgt agagcgccgg gacaggccgc
     4381 tggacctcga catcgccgcc ttgatctgct ctcccgcgga ggtagccgcc cttcacgccg
     4441 cgccccgatc caggcaaaga gagaagctgc tgcaactttg ggtcagaaaa gaggcgctca
     4501 caaaagccgt tggccggggt ctttcgatgg cgctgccgct gattgaactc gatgatgatg
     4561 gctttactgt gtctggccag tcggtgccgg gaatggagca gccggccgat tggcaggtac
     4621 acctgttgac aatggttccg aacgtcttgc tggctatggc ggtacactgc caagggcagc
     4681 acctctgtat ccggcacacc cggatcgact gggtgcgact ctgcgagttt tgtgggattg
     4741 ctacagaatg cgtcaacgta tgactggtgc cagcgcggtc gctttcagtt tcgattgcag
     4801 tgcccggccc ttgcgcgcgc gtttgccgat gtgcaggctt aacgccgtta ccgacagtgc
     4861 aacttcctgc accctgccgc cgcggccggt gcctgacacc agcacacctt gctgcgtgat
     4921 ggcttgcgcc gccagcagtt tttcgttttt ttccagctcc atcagaatca cgccgcggcc
     4981 accgttggtc agcgtcttga gt
//
LOCUS       DASRDH010000293         2871 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_291640,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000293 DASRDH010000000
VERSION     DASRDH010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2871
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291640"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="VFA14_06825"
     CDS             <1..505
                     /locus_tag="VFA14_06825"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020748201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HZS81121.1"
                     /translation="RKVARTIREDVPTEYFGAPLAGGLIPWIDAQLENGQSREEWKGQ
                     AEVNKILGNEKIIPVDGLCVRIGAMRCHSLALTIKLKRDLPLADIASIIRSGNQWVKW
                     VPNERAITVKELTPASITGALEIGVGRVRKLNQGPEYISAFVIGDQLLWGAAEPLRRM
                     LRIILDK"
     gene            682..>2871
                     /locus_tag="VFA14_06830"
     CDS             682..>2871
                     /locus_tag="VFA14_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007884175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV/HubP family polar landmark protein"
                     /protein_id="HZS81122.1"
                     /translation="MSLHLHTNHKFPLLRLKKLNVVITLALALPVGAHAAELGKLTVL
                     SSLGQPLHAEIELNSVSKEEEGALVARLASVDVFKKANIGSDPVLSSLQFSIEQRQGK
                     QLVRIMSLQAINEPFVDLQLELSGNGSRLVREYTFLLDPPDLRRPQVAPVSPGVQTVA
                     AALPVVNDNDEEPISSISTKSAPSSLAEEVIRNGYSSSQGGLTNAPVPAAVAARTVPT
                     VARPVANSVSAVGKRRNRHSAAVAKTAKPEGGSYRVKKGDTLAGIARRTKDGAVSLDQ
                     MLVALYRANPDAFIGENMNWLRAGRILSVPDAATAGAVDNGEARATVVTQARDFNAYR
                     HTLAEQAVSGSIGKARTAKQSDSGKITARVKEKSGTSVARDKLQLSKADAAVDQAAAE
                     SVAAEDRIAADKAIAEANARVKALEKNVSDLEKLLEIKNKMLFEMSEQQNSAAATPET
                     PVPQAAPVDAAGLGEKLGEGSSSEIVKPDVPAPAAPVAEKPVQQPKITVAPDPPPVAK
                     ASFFDNIRNNSYLLPGVSVLVALLAAWAAVRVRNNRKKSAAGVAKADADADKEGEPVK
                     ADKTARPSSTTAVAAVTLATAVTTTEAVTEKTQAVPQVTADAPVAGKARESAPELSAS
                     KKEQGLEFDLSDFKGAEIQPGTTPVAGIDHKTDLDQELDRSAKVDNSIAILLDDGLPT
                     LNDIDLDLPAPVKPIKADKLEDDASAVATEMSAKLDLARAYQNIGDKD"
BASE COUNT          679 a          764 c          853 g          575 t
ORIGIN      
        1 ccgcaaggtt gccaggacca ttcgcgaaga tgtgccaacc gaatatttcg gtgcgccatt
       61 ggccggcggc ttgatcccct ggatcgacgc gcagctggaa aatggtcagt ccagggagga
      121 gtggaaggga caggccgaag tcaacaaaat cctcggaaac gagaagatca tccctgtcga
      181 tggcctgtgc gtgcgtatcg gcgcgatgcg ctgccatagt ctggcattga ctatcaaact
      241 caaacgcgat ctgccattgg cagacattgc atccattatc cgttccggca accaatgggt
      301 gaaatgggtg ccgaatgagc gcgccatcac cgtcaaggag ctgacgccgg catccatcac
      361 cggcgctctg gaaatcggtg tcggccgtgt gcgcaagctg aaccaggggc cggaatatat
      421 ttctgccttc gtgattggcg accagttgct gtggggcgct gctgagccgc ttcgtcgtat
      481 gttacgcatc atccttgaca agtgactgcg tggaaaattg ttccttccgt tgtcagtttg
      541 caacgtcaac ccgccaaata ttttcacaaa atggcgggtt tttgtcatct tgataatggg
      601 gttgtactag tatctatgag atgaaatgca cttttgtttt tttggtcttc tctaacagaa
      661 cgtcgatttc accatacaaa tatgtcccta catctgcata cgaatcacaa gtttccgcta
      721 cttcgtctga aaaaacttaa cgtcgtaatc actcttgcat tggctttgcc ggtgggggcg
      781 cacgccgccg aactgggcaa gctgacggtg ctatcgtcgt tgggtcagcc gctgcacgcg
      841 gaaatcgagt tgaactcggt atcgaaagaa gaagaaggtg cgctggttgc ccggttggca
      901 tctgtagatg ttttcaagaa ggccaatata ggttccgatc cggtgctatc gtcgttgcag
      961 ttttccatcg agcaacgtca gggcaagcag ctagtccgca ttatgtcctt gcaggcaatc
     1021 aatgagcctt tcgtcgattt gcaactggaa ttgagtggca acggttcgcg tttggtgcgc
     1081 gaatatactt ttttgctcga tccgccagac ctgcgcaggc cgcaggtagc gccagtttct
     1141 ccgggagtgc agactgtcgc tgcggccctc cccgtcgtga atgataacga tgaagagccg
     1201 atttccagca tctccaccaa atctgcgccc tccagcctgg cagaggaggt gatccgcaat
     1261 ggttacagca gcagtcaggg cgggttgacc aatgcgccgg taccagcagc ggtagccgca
     1321 aggactgtgc cgacagtcgc cagacctgtg gcaaattcgg tatctgcggt cggcaagcgt
     1381 agaaataggc acagtgccgc cgtcgcgaaa actgccaaac cagaaggcgg atcgtaccgc
     1441 gtgaaaaaag gtgatacgct ggcgggaatc gccaggcgca ccaaagacgg cgccgtgtcg
     1501 ctggatcaga tgctggttgc cttgtatcgc gccaacccag atgctttcat tggtgagaac
     1561 atgaattggc tgcgtgccgg tcgcatattg tcggtgccgg atgctgccac ggcgggtgcc
     1621 gtcgacaatg gtgaggcgcg tgccaccgta gttacgcagg cacgagactt caatgcgtat
     1681 cgtcatacac tggccgaaca ggccgtcagc ggttcgatcg ggaaggcccg taccgcaaag
     1741 cagagcgaca gcggcaaaat caccgcgcgg gtaaaagaaa agtctggcac cagcgtcgcc
     1801 agggataagc tacagctatc gaaggccgac gccgccgtcg atcaggctgc tgccgagagc
     1861 gtggcagcag aagacaggat cgctgccgac aaagccattg ccgaagccaa tgcgcgcgta
     1921 aaagcgctgg agaaaaacgt cagcgacctg gaaaagctgc tggagatcaa gaacaagatg
     1981 ttgtttgaga tgagcgaaca gcagaattct gccgccgcta caccggaaac gccggtgccg
     2041 caagctgcgc cggttgatgc agcaggactg ggtgaaaaac tgggcgaggg cagctcttcc
     2101 gaaatcgtca aaccggatgt ccccgcaccg gcagcgcccg tggcggagaa accagtgcag
     2161 cagccgaaga taaccgttgc gccggatccg ccgccggtcg ccaaggctag tttcttcgac
     2221 aatattcgga acaattccta tctgttgcct ggcgtcagtg tgttggtcgc gctgctggct
     2281 gcatgggctg cggtacgtgt tcgcaataac cggaagaagt ccgctgctgg cgtggccaag
     2341 gctgacgccg atgcagacaa ggagggtgag ccggtcaagg cagataaaac agcccgaccg
     2401 tcgtctacaa cagccgtcgc agcagtaacg ctagcgactg ctgtaactac aacagaagca
     2461 gtgactgaaa aaactcaagc ggtgccgcag gttaccgccg atgcgccggt agctgggaaa
     2521 gcgcgggagt ccgcgccgga attgtcggcg tcgaaaaaag agcagggtct ggaattcgat
     2581 ctgagcgatt tcaagggtgc ggaaatccag ccggggacaa cgccagtggc cggcatcgac
     2641 cacaagaccg atctcgatca ggagctcgat cgcagcgcca aggttgataa cagtatagca
     2701 atcctgctgg acgacggctt gccgacgctc aacgatatcg atctcgattt accagcgccg
     2761 gtcaagccca ttaaagcaga taagctggaa gatgacgcat ctgccgtcgc tacggaaatg
     2821 tcggctaaac tggatctggc tagggcttac cagaacatcg gcgacaagga c
//
LOCUS       DASRDH010000294         3634 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_656142,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000294 DASRDH010000000
VERSION     DASRDH010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 3634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3634
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_656142"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1022
                     /gene="dgt"
                     /locus_tag="VFA14_06835"
     CDS             <1..1022
                     /gene="dgt"
                     /locus_tag="VFA14_06835"
                     /inference="COORDINATES: protein motif:HMM:TIGR01353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dNTP triphosphohydrolase"
                     /protein_id="HZS81123.1"
                     /translation="ACLAHDIGNPPFGHSGEAAIQSWFKKNEEQYLSGLNSCQRNDFL
                     RFEGNAQGFRLITRLQGVHRGGLQLTYAVLAAFGKYPRPSQFPKSEKISEKKFDYCAD
                     DVDTFRKVAEGVGLISKDGAAYCRHPLAFLMEAADDICYRIVDLEDGHRLGRIQFEKA
                     RELLWTIAFPPCKAERNSYKEIDDKDEKIAYLRAKAIDNLIKAVVDVFKKHYEAIMSG
                     NFEEELVKKCKFVSEFDAIKKISQSDIYTTPAVLQIEVAGFGILGGLLDKIVPALLAP
                     EDSRTEEQKKIAALVPEKCKLKDKTAYQRLLGATDYISGMTDTFALSLFRRLHGIELP
                     GGINP"
     gene            complement(1162..1812)
                     /locus_tag="VFA14_06840"
     CDS             complement(1162..1812)
                     /locus_tag="VFA14_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007881405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HZS81124.1"
                     /translation="MATIKDFSALKSLRKDLKAREETRLATEHERIATENRLHAETII
                     FRNGVGKVTPLRCNEKTMLIAPPPLPIARLHIADEQAALMESLSDEFTFDPLPDTDEH
                     LSFARPDASSDVLSKLRRGHWPIQSQLDLHGLRRDEARETLGTFLRQAHHRGQRCVRI
                     IHGKGLGSVNKGPVLKQKVRNWLMQKEEVIAFCQARPADGGSGALLVALRQPDGPA"
     gene            complement(1859..2812)
                     /gene="trxB"
                     /locus_tag="VFA14_06845"
     CDS             complement(1859..2812)
                     /gene="trxB"
                     /locus_tag="VFA14_06845"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017454629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HZS81125.1"
                     /translation="MTTSRHAKVLILGSGPAGYSAAVYAARANLDPVLITGVEQGGQL
                     MTTTDVENWPGDPMGVQGPELMQRLLQHAERFKTDIIFDHIHTTRLSEKPFRLLGDSG
                     EYTCDALIIATGASAQYLGLPSETAFMGKGVSACATCDGFFYRGKEVAVVGGGNTAVE
                     EALYLSNIASKVTVIHRRDKFRAEPILIDRLLHKVAEGKVDIKWQHTLEEVTGDDSGV
                     TGIRIRSTQDSSMTSLGLHGLFIAIGHKPNTDIFAGQIDMHNGYIKTRAGLEGFATAT
                     SIDGVFAAGDVQDHIYRQAITSAGTGCMAALDAQRYLEGLE"
     gene            3157..>3634
                     /locus_tag="VFA14_06850"
     CDS             3157..>3634
                     /locus_tag="VFA14_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HZS81126.1"
                     /translation="MTKTSQAYTRHTKAAAPPLPGRLVRLLYEARWLTLMTLLIYLTL
                     ILLTYSSSDPGWSATSTVSHLHNWGGRVGAWLANLMLYIFGMSAWWWCVLLAHSTWRG
                     YRCLSHRFLVPRPEPGHRQEGLVRAIGFVFLLSGSLGIEYMRMYSLEAQMPRAPGGV"
BASE COUNT          735 a          908 c         1101 g          890 t
ORIGIN      
        1 cagcttgttt agcccatgat atcggtaatc caccatttgg acattctggc gaggcggcga
       61 tccaatcgtg gtttaagaaa aacgaggagc aataccttag tggccttaac agttgccagc
      121 gcaatgactt ccttcgtttc gaaggcaacg cgcaagggtt taggcttatc actcggttgc
      181 aaggtgtcca tcgcggcggt ctccagttga catatgctgt gttggctgct tttgggaagt
      241 atccccgacc atcgcagttt ccaaagtccg agaagataag tgagaagaaa tttgactatt
      301 gcgccgatga tgttgacacc ttcaggaagg tggcagaagg cgttggcctg atttcgaagg
      361 atggcgctgc atactgccgt caccctcttg cgttccttat ggaggctgcg gacgatattt
      421 gttaccgcat cgtggatctc gaagatggtc atcgccttgg ccgtatccag ttcgaaaagg
      481 cacgtgagct tctctggacg attgcattcc ctccatgtaa agccgaaaga aattcctata
      541 aagaaattga tgataaagat gaaaaaattg catatctccg ggctaaagct atcgataact
      601 taattaaagc agttgttgat gtgtttaaga agcactacga ggctatcatg agcggtaact
      661 tcgaagagga gctggtcaaa aaatgtaagt ttgtgtctga atttgacgca attaaaaaaa
      721 tctctcaatc tgacatttac actactcccg cagtactaca gattgaggtt gccggctttg
      781 gaattcttgg gggattactt gacaagattg tccctgctct attggcgccg gaagatagta
      841 gaactgaaga gcaaaagaag atagcagctc ttgttcccga gaaatgcaag cttaaggata
      901 agacagccta tcagcgtttg ttgggagcga ccgactacat ctcgggaatg acagatacct
      961 ttgcgctatc cctgttccgc cgactgcatg gaatagaact tccggggggc atcaatccgt
     1021 gataacgaga gagaagttat ttaatctggt gtcttgtcac gcctcagacg tcagataaag
     1081 tctggatgca catgctgtaa cgagttcttc agccgcagga cgtacttggt gcagtttccg
     1141 accggttcag tgtgacagac atcaggccgg gccgtccggt tgccgcagtg cgactagcag
     1201 cgcgccagag ccgccgtctg ccgggcgcgc ctggcaaaag gcgatcactt cttctttctg
     1261 catcagccag ttgcgcactt tctgtttcag taccggtccc ttgttgactg aaccgagtcc
     1321 cttgccatga atgatgcgca cgcagcgctg accacggtga tgcgcctgcc gcagaaatgt
     1381 gccgagcgtc tcacgcgctt cgtcgcggcg caagccgtgc aggtcgagct gactctggat
     1441 aggccagtga ccacggcgca gtttgctgag aacatcacta ctggcgtcgg ggcgggcgaa
     1501 gctgaggtgt tcatcggtat cgggcagggg gtcgaaagtg aattcgtcgg agagtgattc
     1561 catcaatgcc gcctgctcat cggcgatatg caagcgcgcg atcggcaagg gcggcggtgc
     1621 aatgagcatg gttttttcat tgcagcgcag cggcgttacc ttgccgacgc cgttgcgaaa
     1681 aataatggtc tctgcatgca ggcggttttc ggtggcgatg cgctcatgct cagtggccag
     1741 gcgggtttct tcccgtgcct tgagatcctt gcgcagagac ttgagcgcgg aaaagtcctt
     1801 gatagtcgcc atgatgtgcg aaccagtgag gagatgcttc gcaccgggtc gggagcgttt
     1861 attccaggcc ttccaggtaa cgctgggcat cgagtgcggc catgcagccg gtgccggcgc
     1921 tggtgatggc ctggcggtag atgtgatctt gcacatcacc ggcggcgaac acgccgtcga
     1981 tgctggtcgc ggtggcgaag ccttctagtc cggcgcgggt cttgatgtag ccgttgtgca
     2041 tgtcgatctg gccggcgaag atgtcggtat tgggtttgtg accgatggcg atgaacaggc
     2101 cgtgcaggcc gagcgatgtc atgctgctgt cttgtgtcga tctgatcctg atgccggtga
     2161 cgccactgtc atcgccggtg acttcttcga gcgtgtgctg ccatttgatg tcgaccttgc
     2221 cttcggcaac tttgtgcagc aggcggtcga tcaggatcgg ttcggcgcgg aatttgtcgc
     2281 ggcggtgaat gacagtgact ttgctggcga tgttggacag atacagcgct tcttcgactg
     2341 cggtattccc gccgccgact acggcgactt ccttgccgcg atagaaaaag ccgtcgcagg
     2401 ttgcgcaggc cgatacgcct ttgcccatga aggcggtttc cgaaggcagg ccgaggtact
     2461 gcgccgatgc gccggtggcg atgatgagcg cgtcgcaggt atattcgcca gagtcgccga
     2521 gcagacggaa tggtttttcc gacagtctgg tggtgtggat gtggtcgaag atgatgtcgg
     2581 tcttgaaacg ttctgcatgt tgcagcaggc gctgcatcag ttccgggcct tgcacgccca
     2641 tcgggtcgcc tggccagttc tcgacgtcgg tggtggtcat caactggccg ccttgctcga
     2701 cgccggtgat cagcaccgga tcgaggttgg cgcgcgcggc atagacggca gcgctgtagc
     2761 cggcggggcc ggaaccgaga atcaaaacct tggcatgtct ggacgtggtc ataaaatatt
     2821 cttggtgaac tggacggaag tgagcgcggc agcgatgtag aggaccgaat gccgatccgg
     2881 tagatgtcga tgtggtgcag agagtgtttg ttatattgcc ttttgtcctg tagtttatcc
     2941 tgttttgcgc tgcaaaacgg ttaagattat aggtgagccg ccaaactgtc ccgatggaat
     3001 tgttctatcc ggtcgataga gttggaacag gggcaaaggg gcctggtttc ctgccgccgt
     3061 ttttctggct ggcaatcgcc gttacaatat tgcctgagtt ccccggaagt gagatgtgcg
     3121 tgagtcaagt attggctgcg cgtcagatga agatttatga ccaagaccag ccaagcctat
     3181 acccgtcata ccaaagccgc cgcgccgcca ctgcccggca gactggtgcg tctgttatat
     3241 gaggctcgct ggctgacgct gatgaccttg ctgatttatc tcacgctgat tttgctgacc
     3301 tattcgagca gtgatcctgg ctggtcggcc accagtacgg tgtcgcatct gcataactgg
     3361 ggcggtcgcg tcggcgcctg gctggctaat ctgatgctgt acattttcgg catgtcggcc
     3421 tggtggtggt gcgtgttgct ggcgcattcc acctggcgcg gttaccggtg tttgtcccat
     3481 cgtttcctcg tacccaggcc cgaacctgga catcgccagg aaggtctggt gcgcgccatc
     3541 ggttttgtct ttttgctgtc cggcagtctc ggtatcgaat acatgcgtat gtattccctc
     3601 gaggcgcaga tgccacgtgc gccgggcggc gtgc
//
LOCUS       DASRDH010000295         2484 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1572082,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000295 DASRDH010000000
VERSION     DASRDH010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1572082"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="VFA14_06855"
     CDS             <1..470
                     /locus_tag="VFA14_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HZS81127.1"
                     /translation="RLRGMLFWLMGDLEDSHFLPVAAVIVLLVLLWSAWNAPHLNLLA
                     RGESFAQSLGVPVLRLRVATLCAASLATAAAVIMAGSIGFVGLVTPHCLRLVIGNDQR
                     MLLPAAALAGGSGLALADLAARTVAAPTQLPVGVITALIGVPLFLWLLTRSRA"
     gene            476..1258
                     /locus_tag="VFA14_06860"
     CDS             476..1258
                     /locus_tag="VFA14_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZS81128.1"
                     /translation="MRPTLLTTEKLTLRAGTVSLPVQFDWQVSPGEFWCVLGKNGIGK
                     STLLHTVSGLLPPAGGQVYSDQGNIFQSQPPRQLALWRGLVSQEQFDAFSIRVRDSVM
                     SGLHPHRTGWGWPDEQDRRAALVALRRLGMASHADTDVMRLSGGERQRVAIATLLLQA
                     PRLYLFDEPVSHQDVAAQQKVMLVLRDLADDSHAVVASMHDINLAACFATHILLIGAH
                     RCWQGTTRDVLRPDLLQMAFDCPFEMLESTRGRWLMPVSMTV"
     gene            complement(1255..2160)
                     /locus_tag="VFA14_06865"
     CDS             complement(1255..2160)
                     /locus_tag="VFA14_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011296848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HZS81129.1"
                     /translation="MFEISSRAVQTSFQAVCFLVGSAAAAATHVAVKDDLGNDIRLER
                     PARRIVSLSPHTTELLYAAGAGAHVVGVSNHSNYPPAARQVVSVGNTGAVDIEKILML
                     KPDLIIAWHSGNAAGQLQRLRAFGLPVFESEPTDYATIASSLERLGMLSGSLAAASLA
                     AASFRMRWQALASTYHARAPVSVFYQIWEQPLMTLNGKHMVSSVLRTCGGRNLFADMP
                     QLAPGVSIESVIAADPDVILAPDGAGERTFARWRRFTKMKAVMRSNLLTVHADWLNRP
                     GPRILDATEQVCVLLDTVRHRSAAR"
     gene            complement(2165..>2484)
                     /locus_tag="VFA14_06870"
     CDS             complement(2165..>2484)
                     /locus_tag="VFA14_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HZS81130.1"
                     /translation="FRADDGLALSSRNANLSADERAEAPALYQVLQQVASAVRGGHPD
                     LVGLEHKAMHTLAQRGWKPDYISIRKRHDLQPPSTGDMAQGLVVLAAAKLGNTRLIDN
                     LEV"
BASE COUNT          446 a          700 c          800 g          538 t
ORIGIN      
        1 cccgattgcg cggcatgctg ttctggctca tgggcgatct ggaagatagt cacttcctgc
       61 cggttgcggc agtgatcgtt ttgttggttt tgctgtggtc ggcatggaat gcgccgcatc
      121 tgaacctgct ggcgcgcgga gaaagtttcg cccaatcgct cggcgtgcca gtgctacgcc
      181 tgcgcgtggc gaccttatgc gcggcatcac tggcgacggc ggcggcagtg attatggcgg
      241 gaagcatagg cttcgttggt ctggtgacgc cacactgttt gcggctcgtg atcggcaacg
      301 atcagcgcat gttgttaccg gcggcagcgt tggccggtgg cagcggtctg gcgctggctg
      361 atctcgcggc acgtactgtc gccgcgccga cgcaattacc ggtcggcgtc attacagcct
      421 tgatcggcgt gccgctgttc ttgtggttgc taacgcgcag ccgtgcatga tgccgatgcg
      481 acctaccctg ctcactaccg aaaaactgac tttgcgcgcc ggtacggtgt ccctgccggt
      541 gcagttcgac tggcaagtca gtccaggcga attctggtgt gtgctgggca agaacggtat
      601 tggcaaaagc acattgctgc ataccgtgtc aggtttgctg ccgcctgccg gcggtcaggt
      661 atattcagat cagggaaata tctttcagtc acagccgccg cggcaactgg ctttgtggcg
      721 cggattggtt tctcaagaac agttcgacgc cttctccatc cgtgtacgtg acagcgtcat
      781 gagcggcttg catcctcatc gtaccggctg gggatggccg gatgagcagg accgccgtgc
      841 agcgttggtg gcattgcgca ggctgggcat ggcgtctcat gctgacactg acgtcatgcg
      901 cctgtccggc ggcgagcgtc aacgtgtcgc gatagcgact ttgttgctgc aagcgccgcg
      961 actttatctg ttcgacgagc cagtatcgca ccaggatgtc gccgcgcagc agaaagtgat
     1021 gctggtattg cgcgatcttg ccgatgatag ccacgccgtg gtcgccagca tgcatgatat
     1081 caatctggcg gcgtgttttg ccacgcatat cctacttatt ggcgcgcacc gttgttggca
     1141 gggaacgacg cgcgatgtat tgcggccaga tctgctgcaa atggcattcg attgcccttt
     1201 tgagatgctg gaatccacgc gcggaagatg gctgatgccg gtttcgatga cagtttagcg
     1261 tgcggcggag cggtggcgca cggtgtcaag taacacgcac acttgttcgg ttgcatcgag
     1321 tatccgtggt ccggggcggt tcagccagtc ggcatggacc gtcaataaat tgctgcgcat
     1381 gaccgccttc atttttgtaa aacgccgcca tcgcgcgaag gtacgttccc cggcaccatc
     1441 gggcgcgagg atgacatcgg gatcggcggc gatgacgctt tcgatgctca cgccaggcgc
     1501 cagttgtggc atatcggcaa agaggttgcg gccaccgcag gtgcgcaata ccgacgacac
     1561 catatgcttg ccattgagcg tcatcagcgg ttgctcccat atctgataaa acacgctgac
     1621 cggcgcgcgc gcgtgataag tgcttgccaa cgcttgccag cgcatgcgga agctggctgc
     1681 ggcaaggctg gctgcggcaa ggctgccgct cagcatgccc agccgttcca gtgaagaagc
     1741 aatcgtggcg taatcggtcg gctcgctttc aaaaaccggc aggccgaagg cgcgcaagcg
     1801 ctgcaattgc ccagccgcat tgccgctgtg ccaagcaatg atcaggtcgg gtttgagcat
     1861 gagaattttt tcgatatcga ctgcaccagt attgccgacc gagacgactt gtcgtgctgc
     1921 cggcggatag ttgctatggt tgctgacgcc gacgacatgc gctcctgcac cggcggcgta
     1981 gagcagttcc gtggtgtgcg gcgataggct gacgatgcgg cgcgccgggc gttccaggcg
     2041 aatgtcattg ccgaggtcgt ccttgacggc gacatgtgta gccgcagctg ctgccgaccc
     2101 caccagaaag cagaccgcct gaaaagacgt ttggacggcc ctggaggaga tctcgaacac
     2161 gttctcagac ttccagattg tcgatcaggc gtgtattgcc gagcttggcg gcggcgagaa
     2221 ccaccagccc ttgcgccata tcgccggtgg aaggtggttg caggtcatga cgtttgcgaa
     2281 tggagatata gtctggcttc cagccgcgct gcgccagcgt atgcatggcc ttgtgttcca
     2341 gtccgacgag atccggatgg ccaccgcgca ctgcgctggc gacctgctgt aatacctgat
     2401 acaacgctgg tgcttcggcg cgctcgtccg cggacagatt ggcattgcgc gaggacagcg
     2461 ccaggccgtc gtcggcgcgg aagg
//
LOCUS       DASRDH010000296         3017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1667822,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000296 DASRDH010000000
VERSION     DASRDH010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1667822"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..905)
                     /gene="leuB"
                     /locus_tag="VFA14_06875"
     CDS             complement(<1..905)
                     /gene="leuB"
                     /locus_tag="VFA14_06875"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013957415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HZS81131.1"
                     /translation="MKIAVLPGDGIGPEVIEQAVRVLDALGEKFEMETAPVGGAGYEA
                     HGRPLPDNTLKLAREADAILFGAVGDWKYDRLDRPLRPEQAILGLRKHLQLFANFRPA
                     ICYPELASASSLKPELVAGLDILIVRELNGDVYFGQPRGMREAPDGPFKGAREGFDTM
                     RYSEPEIRRIAHVAFQAATRRSNHLCSVDKANVLETFQFWKDIVTDVHKEYPQVELSH
                     MYIDNAAMQLVKAPKNFDVIVTGNLFGDILSDAAAMLTGSIGMLPSASLDADNKGLYE
                     PSHGSAPDIAGRGIANPLAAILSAA"
     gene            complement(1005..1652)
                     /gene="leuD"
                     /locus_tag="VFA14_06880"
     CDS             complement(1005..1652)
                     /gene="leuD"
                     /locus_tag="VFA14_06880"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HZS81132.1"
                     /translation="MNKFTVHDGLLAPLDRANVDTDAIIPKQFLKSIRRSGFGPNLFD
                     EWRYLDRGEPGMDNGKRPLNPDFVLNQPRYQGASILLSRNNFGCGSSREHAQWALSQY
                     GFRAVIASSFADIFFNNCFKNGLLPIVLQEQQIDHLFDEVKAFPGFRLIIDLEKQIIT
                     TANNSQIYPFEVGEFRKYCLLNGLDDIGLTLRHADKIRVFEERRLHDQPWLANTL"
     gene            complement(1723..>3017)
                     /gene="leuC"
                     /locus_tag="VFA14_06885"
     CDS             complement(1723..>3017)
                     /gene="leuC"
                     /locus_tag="VFA14_06885"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008330870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HZS81133.1"
                     /translation="SAQAFESLRLAGRTVRRPDRTLATADHNVPTVDRNQLWPDPLSI
                     RQVDVLRQNVKEFGVPYFGIEDRRQGIVHVIGPEQGATLPGMTVVCGDSHTSTHGAFA
                     CLAHGIGTSEVEHVLATQTLLAKKSRSMLVQVDGALSRGVTAKDIVLAVIGRIGTAGG
                     TGYAIEFAGSTIRSLSMEGRMTVCNMAIEAGARAGMVAVDDTTINYLKGRPLAPSGVH
                     WERAVSYWRTLHSDPGARFDMVVKFDVAEIKPQVTWGTSPEMVVAVDSRVPDPDKEKD
                     PTRRDGMEKALAYMGLKPDMLIGEIRIDKVFIGSCTNSRIEDLREAAAVVRGRFRASS
                     VKLAMVVPGSGLVREQAEREGLDRIFRDAGFEWREPGCSMCLAMNADRLEPGERCAST
                     SNRNFEGRQGAGGRTHLVSPAMAAAAGIAGHFVDVRSL"
BASE COUNT          645 a          915 c          848 g          609 t
ORIGIN      
        1 atcgccgccg atagaatggc cgccagcgga ttggcaatgc cccggccggc aatgtcaggc
       61 gctgaaccat gcgacggttc atacaagccc ttgttatcgg cgtccagcga tgccgatggc
      121 aacatgccga tcgagccggt caacatggct gccgcatccg acaagatgtc accaaacagg
      181 ttgccggtaa cgatgacgtc gaagttcttc ggcgccttga ccagttgcat tgccgcgttg
      241 tcgatataca tgtgagacag ttcgacttgc ggatattcct tatgcacgtc ggtgacgatg
      301 tctttccaga actgaaaggt ctccagcaca ttggctttgt cgacgctgca caggtgattg
      361 ctgcgcctag ttgcggcctg gaaggcgacg tgcgcgatgc ggcggatttc cggctcgcta
      421 tagcgcatgg tgtcaaagcc ctcgcgcgcg cccttgaacg ggccgtccgg cgcttcacgc
      481 atgccgcgcg gctggccgaa ataaacatcg ccattcaact ctcgcacgat caaaatgtcc
      541 agaccggcca ccagctcagg ctttagcgac gatgcgctgg ccagttccgg ataacagatc
      601 gcgggacgga agttagcgaa cagttgcaga tgcttgcgca gaccgagaat ggcctgctca
      661 ggacgcagcg ggcgatccag cctgtcgtac ttccagtcac cgacggcgcc gaacaggatg
      721 gcatcagctt ccctggccag cttcaaggtg ttgtccggta acggacgacc gtgcgcctca
      781 taacctgcgc cgccgaccgg tgcggtctcc atctcaaatt tttcacccag ggcatccagt
      841 acacgtaccg cttgctcaat gacctcggga ccaatgccat cacccggcaa aactgcaatt
      901 ttcatgtcat cttctcgttt tgaaaaaatc agactgccgc acctgcgcca atcttcacga
      961 tcgcgcgatg cgacacccag ctcagctacg ttcttggata gctcttagag agtattggcc
     1021 aaccagggtt gatcgtgcaa acgacgctct tcaaagacac ggattttgtc agcatggcgc
     1081 aatgtcagac cgatgtcgtc gaggccgttg agcaaacagt atttacggaa ttcaccgact
     1141 tcaaacggat aaatctgact attattcgcg gtagtaataa tttgcttttc cagatcgatg
     1201 atcaggcgaa agcccgggaa tgctttcact tcgtcgaaca gatggtcgat ctgctgctcc
     1261 tgcagcacga taggcagcaa accgttcttg aagcagttat tgaagaaaat gtcggcgaaa
     1321 cttgacgcga tcacagcgcg aaaaccgtat tgactcagcg cccactgcgc gtgttcgcgc
     1381 gaggagccac aaccgaagtt gttacgcgat agcaggatcg aggcgccttg atagcgcggc
     1441 tgattcagta cgaaatcagg gttcagcgga cgcttgccat tgtccatgcc tggttcgccg
     1501 cgatccaggt aacgccattc gtcaaacaga ttcgggccga aaccgctgcg tctaatcgac
     1561 ttcagaaatt gcttgggaat gatcgcgtcg gtatcgacgt tagcgcggtc aaggggagca
     1621 agcaaaccat catggacggt gaatttattc atggcactcg atatcgataa gggagcgccc
     1681 gccatgcggg aacgctggtc aacctgattt caacaaagtt atctacagag aacggacatc
     1741 cacaaaatga cctgcaatgc cggctgccgc agccatggcc ggactcacca gatgggtgcg
     1801 tccgccggca ccctgccgac cctcgaaatt acggttcgat gtcgacgcgc agcgctcgcc
     1861 cggctctaga cggtcggcgt tcatcgccag gcacatcgag caacccggct cacgccattc
     1921 aaaaccggca tccctgaaga tcctgtccaa cccttcgcgt tcggcctgct ccctgaccag
     1981 gccagaaccc ggcaccacca tcgccagctt gacgctagaa gcgcggaacc tgccgcgcac
     2041 taccgctgcc gcttcgcgta aatcttcgat gcgcgagtta gtgcaagagc cgatgaacac
     2101 cttgtcgata cgaatctctc cgatcagcat gtcaggcttg agacccatgt aagccaaggc
     2161 tttttccatg ccgtcgcgcc tggtcggatc tttttccttg tccgggtccg gcacacgact
     2221 gtcgacggcg accaccattt ccggcgatgt gccccaagtc acttgcggct tgatctcagc
     2281 gacatcaaat ttcacgacca tgtcgaacct ggcgccgggg tcggaatgca gtgtccgcca
     2341 gtaactcacg gcgcgctccc aatgcacgcc agacggtgcg agcggacggc ccttgagata
     2401 attgatcgtg gtgtcgtcga cggccaccat gccggcgcgc gcgccagctt cgatggccat
     2461 attgcagacc gtcatgcggc cttccataga cagcgaacgg atggtggagc cagcaaattc
     2521 gatggcgtaa ccggtcccac cggcagtacc gattctaccg atcacggcca gcacgatgtc
     2581 ctttgccgtc acgccgcgcg acagcgcgcc gtcgacctgt accagcatgg atctggattt
     2641 tttcgccagc agcgtttgcg tggccagaac gtgttcgact tcagaggtgc cgataccgtg
     2701 cgccagacag gcgaaagcgc cgtgtgtgga agtgtgtgaa tcaccgcata ccaccgtcat
     2761 gcccggcagc gtcgcgccct gctcgggtcc aatgacgtgg acgatgccct ggcgtcgatc
     2821 ttcgatcccg aaatacggga ccccgaactc cttcacgttc tggcgaagga cgtcgacctg
     2881 cctgatggaa agcgggtccg gccagagctg gttgcggtcg accgtgggga cgttgtggtc
     2941 cgcggtggcg agggtgcggt ccggacgccg cacggtacgc ccggccaggc gcagcgactc
     3001 gaaggcctgc gcgctcg
//
LOCUS       DASRDH010000297         2216 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_635945,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000297 DASRDH010000000
VERSION     DASRDH010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_635945"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="VFA14_06890"
     CDS             complement(<1..277)
                     /locus_tag="VFA14_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007881020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA/Hda family protein"
                     /protein_id="HZS81134.1"
                     /translation="MRQLLLDLGAQQAPTFAGFVTGRNAELLQRLQGIARAQEQTALH
                     DRFVYFWGESGAGKTHLLHALARANGDSRLIAADAPDSAFDYTPAVQC"
     gene            complement(274..1362)
                     /locus_tag="VFA14_06895"
     CDS             complement(274..1362)
                     /locus_tag="VFA14_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017879371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HZS81135.1"
                     /translation="MSFSFSDEQKQSLPWFLVGLLLAGILVLLGPMLTPFVVAAILAY
                     ALNPGVDWLATRRAGRFYLPRALAVLIVILLLMLTVLALILVVMPVLLKEIPLLQRQI
                     PDFLDKLNATVAPHLGEYGIHVRLDIIGIKKILTQQLVTNGDEIWAPVLASLKTGGSA
                     LLTWLVNLLFIPMLLFYLLQDWHPFIERLRHMIPRRWVGKISHMAEEVDDLLAQYLRG
                     QLLVMLILAAYYSIALGIVGFDIALPVGILTGVLVFIPYVGFGLGLTLALIAAVLQFT
                     SLYGLMAVAIIYGVGQVVESFILTPQLVGERIGLHPLVVIFALLAFGQLFGFVGVLLA
                     LPASAIVSVGVRHLRQRYLSSSFYNREV"
     gene            1476..>2216
                     /gene="purM"
                     /locus_tag="VFA14_06900"
     CDS             1476..>2216
                     /gene="purM"
                     /locus_tag="VFA14_06900"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006461774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HZS81136.1"
                     /translation="MTSSVSLSYRDAGVDIDAGDALVEAIKPFARRTAREGVLGGIGG
                     FGALFEISRKFKEPVLVSGTDGVGTKLKLAFHLGRHDTIGIDLVAMSVNDILVQGAEP
                     LFFLDYFACGKLDVPSATDVIKGIARGCEQAGCALIGGETAEMPGMYPDGEYDLAGFA
                     VGVVEKSRLIDGSRITAGDVVLGLASSGAHSNGYSLVRRILEAAGPDLNADFHGRRLA
                     DVLLEPTRIYVKPLLALMASMEVKGMVHI"
BASE COUNT          479 a          633 c          675 g          429 t
ORIGIN      
        1 agcattgcac ggcgggcgta tagtcgaaag cgctgtcggg cgcatcggcg gcaatcaggc
       61 ggctgtcgcc attggcgcgg gccagagcat gcagcaggtg tgtcttgccc gcgccgctct
      121 cgccccagaa atagacgaaa cggtcatgca aggcggtttg ctcctgcgcg cgggcgatgc
      181 cttgcagccg ttgcagcaac tcggcattgc gcccggtcac gaagccggcg aaagtcggcg
      241 cctgctgcgc gcctaaatcc agcagaagtt gtctcatacc tctctattat agaaactgct
      301 actcagataa cgctggcgca gatggcgcac ccctaccgaa acgatggccg aggcaggcag
      361 ggccagcagt acgccgacga aaccgaatag ctgcccgaac gccagcaggg cgaagatcac
      421 caccagcgga tgcaggccga tacgttcgcc gaccagttgt ggcgtcagga tgaagctttc
      481 caccacttgg ccgacgccat agatgatggc tacggccatc aagccgtaca ggctggtgaa
      541 ctgcaatacg gcggcgatca gcgctagcgt cagaccaagg ccgaagccga cataaggaat
      601 gaagaccagc acaccggtca ggatacccac gggcaaggcg atatcgaagc cgacaatgcc
      661 tagcgcgatc gaataataag ccgccaggat caacatcacc agcaattgcc cgcgcagata
      721 ttgcgccagc aggtcatcga cttcttcggc catatggctg atcttgccaa cccagcgacg
      781 tgggatcatg tggcgcaggc gctcgatgaa ggggtgccag tcctgcaaca gataaaacag
      841 cagcatggga atgaacagca gattgaccag ccatgtcagc agcgcgctac cgccggtttt
      901 gagcgaggcc agcaccgggg cccagatctc atcaccgttg gtgactagct gctgggtcag
      961 gattttcttg atgccgatga tatccaggcg cacgtggatg ccgtattcgc cgagatgcgg
     1021 cgctacagtg gcgttgagtt tgtcgagaaa atcgggaatc tgccgttgca gtagtgggat
     1081 ttccttgagc agcaccggca tgacgaccag aatcagagcc agtactgtca gcatcagcag
     1141 caggatgacg atcagcaccg ccagcgcgcg cggcagataa aaacggccag cacggcgtgt
     1201 ggccagccag tcgacacccg gattgagcgc atacgccagg atggcggcga cgacgaaggg
     1261 cgtcagcatt ggccccagga gtaccaggat gcctgccagc agcagcccta cgagaaacca
     1321 tggcaagctt tgtttttgct catccgagaa agaaaaagac atggaaatcg ggctttgggc
     1381 gaatcggttt gataaaatca cggttttgtt ttcgcagcct tgccggccgc ctcgtcccgc
     1441 tattctacct taccgtttcc agtatccatt tcatcatgac ctcatctgtt tctctttctt
     1501 accgcgacgc cggtgtcgat atcgatgccg gcgatgcttt ggtcgaggcg atcaagcctt
     1561 ttgccaggcg tactgcccgt gaaggcgtgc tgggcggcat aggcggtttc ggcgccttgt
     1621 tcgagatcag caggaagttc aaggaacctg tcctggtgtc cggtaccgat ggcgtcggca
     1681 ccaagctcaa gctggctttt cacctcggca ggcacgacac catcggcatc gatctggtcg
     1741 ccatgagcgt caacgacatt ctggtgcagg gtgccgaacc gttgtttttc cttgattact
     1801 ttgcctgtgg caagctggac gtgccaagcg ctacggatgt catcaagggc atcgccaggg
     1861 gctgcgaaca agctggctgc gcgctcatcg gcggtgaaac cgctgaaatg ccgggcatgt
     1921 atccggacgg cgaatacgat ctggccggct ttgccgtcgg ggtggtcgaa aaatccaggc
     1981 tgatcgacgg cagcaggatt actgcgggtg acgtggtgct gggcctggct tcttctggcg
     2041 cgcactccaa cggttactcg ctggtacgca ggattctgga agcggccgga cccgacctga
     2101 acgccgactt ccacggccgc aggctggctg acgtactgct ggagccgacc cgcatttatg
     2161 tcaagccgct gctggccttg atggcttcga tggaagtgaa gggcatggtt catatt
//
LOCUS       DASRDH010000298         3331 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1323565,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000298 DASRDH010000000
VERSION     DASRDH010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 3331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3331
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1323565"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="VFA14_06905"
     CDS             <1..435
                     /locus_tag="VFA14_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007877668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HZS81137.1"
                     /translation="AASASVSRSALEARKLGYLRADDVIVFNPYELLHVAKVEARAMS
                     DTGYRPPLAPQVPVVGRYGVGTIMAQLVNMRDGGFISAHDFRLGQMIAEIVSGGVVEE
                     GSVVSEQWLLDLERKAFMELLNHPKTQERIMGMMQTGKPVRN"
     gene            469..1665
                     /locus_tag="VFA14_06910"
     CDS             469..1665
                     /locus_tag="VFA14_06910"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HZS81138.1"
                     /translation="MTRQLQDAYIVAATRTPIGKAPHGMFRNVRPDDLLVRVIQSAMA
                     QVPGLDPQLVEDAIIGCSFPEGAQGLNMARIAVLLAGLPDTVGGVTVNRYCASGITAV
                     AMAADRIRAGQADVMIAGGAESMSMVPMRGFHPSINMTALQDENIGMAYGMGLTAENV
                     ANRWKITREAQDAFALASHQKASAAQLAGEFDDEMTPFDIIERFPNLASGQIDAKTRT
                     VNRDEGVRVDSTIEALAKLKPAFAVKGSVTAGNSSQTSDGAGALILVSEKILKQCKLT
                     PLARFVSFAVRGVPPEIMGIGPKEAIPIALRMAGIAQDQLDWIELNEAFAAQALAVMQ
                     DLGLDPSRVNPMGGAIALGHPLGATGAIRAATVIHAMRRKNLKYGMVTMCIGTGMGAA
                     GIFERL"
     gene            complement(1746..2681)
                     /gene="hprK"
                     /locus_tag="VFA14_06915"
     CDS             complement(1746..2681)
                     /gene="hprK"
                     /locus_tag="VFA14_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006465120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HZS81139.1"
                     /translation="MPAYTALSIQQLYEENRESLQLGWFAGFPGGERLISGDAVSAAD
                     QVGHLNLIHPGRIQVFGHQETEYYQRLSNTSRAYQIVELVAGEPPAFIIAQGLATPPD
                     ILTICDEKNIPLFSTPLPAAQVIDYLRIYLSKKLAQQTTMHGVFMDVLGVGVLITGES
                     GLGKSELGLELISRSHGLVADDAVEFARIAPNMIEGRCPPLLQNLLEVRGLGLLDIKA
                     IFGETAVRRKIRLKLIVHLVRRSTLEESYARLPLDAQFEEILGLPIRKVVIPVAAGRN
                     IAVLLEAAVRNTILQLRGIDTLKEFIDRQRKSMDR"
     gene            complement(2744..3217)
                     /locus_tag="VFA14_06920"
     CDS             complement(2744..3217)
                     /locus_tag="VFA14_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007880749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HZS81140.1"
                     /translation="MTNLAKILSPENVILDLEVSSKKRAFEQAGLTFENNSGIARSTV
                     SENLFAREHLGSTGLGHGVAIPHGRIKGLKAPLALFMRLTEPIPFESPDGEPVKLLIF
                     LLIPEHGTQQHLEILSEIAEMFSNHTFRTKLITDADAVSVYSRLVTWQPSFEKNS"
BASE COUNT          705 a          920 c         1002 g          704 t
ORIGIN      
        1 gcggccagcg ccagtgtctc cagatcggcg ctggaagcac gcaagctcgg ctacctcagg
       61 gcggacgatg tcatcgtctt caacccttac gaattgttgc acgtcgccaa ggtcgaagcg
      121 cgtgctatgt ccgacaccgg ctaccgtccg ccactggcgc cgcaagtgcc ggtggtcggt
      181 cgctatggcg tcggcaccat catggcgcaa ctggtcaaca tgcgtgacgg cggtttcatc
      241 tccgcacacg atttcagact gggccagatg atcgccgaga tcgtcagcgg cggtgtcgtc
      301 gaagaaggca gtgtggtcag cgagcaatgg ctgctggatc tggaacgcaa ggctttcatg
      361 gaattgctga atcatccgaa gacccaggaa cgcatcatgg gcatgatgca gaccggtaag
      421 ccggttcgta attgatatac gccgccacgt aaaaatcagg agccgcacat gaccagacaa
      481 cttcaagacg cctacatcgt ggctgcgacg cgtactccga tcggcaaagc gccgcacggc
      541 atgttcagga acgtgcgccc ggacgatcta ctggtgcgcg tgattcagtc cgcgatggcg
      601 caagtccccg gcctcgatcc gcagctggtc gaagacgcca tcatcggctg ttctttccct
      661 gaaggagcgc agggtctcaa tatggcgcgc atcgccgtgc tactggctgg tttgccggat
      721 accgtcggcg gcgtcaccgt caaccgctac tgcgcctccg gcatcacggc ggtggcgatg
      781 gcggcggacc gcatccgtgc cggtcaggcc gacgtgatga tcgccggtgg cgccgagtcg
      841 atgtcgatgg tgccgatgag gggatttcac ccatcgatca acatgactgc gttgcaggac
      901 gaaaatatcg gcatggccta tggcatggga ctgacagcag aaaacgtcgc taatcgatgg
      961 aagatcacgc gtgaagcgca agacgccttt gcgctggcct cgcaccagaa agccagcgct
     1021 gcgcaactgg ctggcgaatt cgacgatgaa atgaccccgt tcgatatcat cgaacgcttc
     1081 ccgaatctgg caagcggcca gatcgacgcc aagactcgca ccgtcaatcg ggacgaaggt
     1141 gtacgcgtcg acagcaccat cgaagcgcta gctaagctga agccggcatt cgccgtcaag
     1201 ggcagcgtca ctgcaggcaa cagctcgcag acttccgacg gcgctggcgc attgatcctc
     1261 gtcagtgaaa aaatccttaa gcaatgcaag ctgacgccac tggcgcgttt cgtctcgttt
     1321 gcggtgcgtg gcgtgccgcc ggaaatcatg ggcatcggtc ctaaggaagc catccccatc
     1381 gccctgcgca tggccggcat cgcgcaggat cagctcgact ggatcgaact caacgaagct
     1441 ttcgccgcgc aggcgctggc cgtgatgcag gatctcggct tggatccctc cagggtcaac
     1501 ccgatgggcg gcgcgattgc gctcggccac ccgctcggcg ccaccggcgc aatccgcgcc
     1561 gcgactgtca tccacgccat gcgccgcaag aacctgaagt acggcatggt gacgatgtgc
     1621 attggcaccg gcatgggtgc ggccggtatt ttcgaacgtc tataaaatca ccaggaacat
     1681 tcctgaccat cgctccgggt ggcgtgacga ggctgttttc aacagtctgc cagaaaagcg
     1741 ccggtttacc gatccatgga cttgcgttgc cggtcgatga attccttgag cgtgtcgatg
     1801 ccgcgcaatt gcaggatggt gttgcgtact gctgcttcca gcagcaccgc gatattgcgg
     1861 ccggctgcta cgggaatcac gacttttcgg atcggcaggc ccagtatctc ttcgaactga
     1921 gcgtccagtg gcagacgtgc gtaactttct tcgagcgtgc tgcggcgaac tagatgcaca
     1981 attagcttca ggcgtatttt acggcgcacg gcggtctcac cgaagatggc cttgatgtcg
     2041 agcaggccga gaccacgcac ttccagcaga ttttgcagta atggcgggca acgtccttct
     2101 atcatgttgg gtgcgatgcg cgcgaactcg acagcgtcat cagccaccag gccatggctg
     2161 cgtgaaatca gttccaggcc gagttcactc ttgcccaggc cggattcgcc ggtgatgagt
     2221 acgcccacac ctagcacgtc catgaagacg ccatgcatgg tagtttgctg agccagtttc
     2281 ttggacaggt aaatgcgtag ataatcgatc acctgcgccg ccggcaacgg cgtagaaaag
     2341 agtgggatat tcttttcgtc gcagatggtc agaatgtccg gtggcgttgc cagaccctgc
     2401 gcgatgatga aggctggcgg ttcgccagcg accagctcga cgatctggta ggcgcgcgac
     2461 gtgttcgaaa gacgctggta gtactcggtt tcctgatggc cgaagacttg aatgcggccc
     2521 ggatgaatca gattcagatg gccgacctga tcggcggctg atacggcgtc accggagatc
     2581 aggcgttctc cgccggggaa gccggcaaac cagcccagct gcaacgactc gcggttttct
     2641 tcatacagtt gctgtatcga taacgcggta taggcaggca tggatctctg aaagatggtg
     2701 gttgcggcag atgagatgca tcagtccggt cgccttgctg tgatcaactg tttttttcaa
     2761 atgatggttg ccaggtgact agacgcgagt agaccgacac ggcatcggca tcggtgatca
     2821 acttggtgcg aaaggtatga ttggaaaaca tctcggcgat ttcggacagg atttccagat
     2881 gctgctgggt tccgtgctcg ggaatcagca ggaagatcag cagtttgacc ggttcaccgt
     2941 caggcgactc gaatggaatc ggctcagtca gacgcatgaa taaggccagt ggtgctttga
     3001 gacccttgat acggccatgc ggaatggcta caccatggcc cagtcctgtt gaaccgaggt
     3061 gttcgcgtgc aaacaggttt tctgaaacag tggagcgcgc aatgccgctg ttgttttcaa
     3121 aggtaagtcc ggcttgttca aatgcccgtt ttttgctgga gacttctagg tccagaatga
     3181 cattttccgg agacaggatt tttgcaagat ttgtcatgac aaaggcgcta tggatgatag
     3241 gcttttgtgc aggattatac tcattgccgt gcatttttca gattagatgg tgcctgttct
     3301 gttgaaaaat tgctacgcca cggattgatg t
//
LOCUS       DASRDH010000299         2975 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_672347,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000299 DASRDH010000000
VERSION     DASRDH010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2975
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_672347"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /gene="rplC"
                     /locus_tag="VFA14_06925"
     CDS             complement(<1..229)
                     /gene="rplC"
                     /locus_tag="VFA14_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875299.1"
                     /note="binds directly near the 3' end of the 23S rRNA,
                     where it nucleates assembly of the 50S subunit; essential
                     for peptidyltransferase activity; mutations in this gene
                     confer resistance to tiamulin; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HZS81141.1"
                     /translation="MSLGLVGRKVGMMRIFMEGGDAVPVTVLDVSNNRVTQIKTPEVD
                     GYSAVQVAFGQRRASRVTKAAAGHYAKAGVEA"
     gene            complement(413..730)
                     /gene="rpsJ"
                     /locus_tag="VFA14_06930"
     CDS             complement(413..730)
                     /gene="rpsJ"
                     /locus_tag="VFA14_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010394389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HZS81142.1"
                     /translation="MSVPSQKIRIRLKAFDYRLIDQSALEIVDTAKRTGAVVKGPVPL
                     PTRIQRFDVLRSPHVNKTSRDQFEIRTHQRLMDIVDPTDKTVDALMKLDLPAGVDVEI
                     KLQ"
     gene            complement(800..1990)
                     /gene="tuf"
                     /locus_tag="VFA14_06935"
     CDS             complement(800..1990)
                     /gene="tuf"
                     /locus_tag="VFA14_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HZS81143.1"
                     /translation="MAKGKFERTKPHVNVGTIGHVDHGKTTLTAAIATVLSKKFGGEA
                     KAYDQIDAAPEEKARGITINTAHVEYETANRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVCSAADGPMPQTREHILLSRQVGVPYIIVFLNKADMVDDEELLELVEMEVRELLSK
                     YEFPGDDLPIIKGSAKLALEGDTGPLGEQAIMALAEALDSYIPTPERAVDGSFLMPVE
                     DVFSISGRGTVVTGRVERGVIKVGEEIEIVGIRETQKTTCTGVEMFRKLLDQGQAGDN
                     VGVLLRGTKREDVERGQVLAKPGSIKPHKHFTGEIYVLSKDEGGRHTPFFNNYRPQFY
                     FRTTDVTGSIELPKDKEMVMPGDNVSITVQLINPIAMEEGLRFAIREGGRTVGAGVVA
                     KIFD"
     gene            complement(2073..>2975)
                     /gene="fusA"
                     /locus_tag="VFA14_06940"
     CDS             complement(2073..>2975)
                     /gene="fusA"
                     /locus_tag="VFA14_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012357144.1"
                     /note="EF-G; promotes GTP-dependent translocation of the
                     ribosome during translation; many organisms have multiple
                     copies of this gene; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HZS81144.1"
                     /translation="VVLERMVFPEPVISQAVEPKTKADQEKMGLALNRLAAEDPSFRV
                     RTDEESGQTIIAGMGELHLDIIVDRMKREFNVEATVGKPQVAYRETIRKTCEEIEGKF
                     VKQSGGRGQYGHVVLKIEPQEPGKGFEFVDAIKGGTVPREFIPAVEKGVRETLNTGVL
                     AGYPVVDVKVTLFFGSYHDVDSNENAFRMAASMAFKDGCRKASPVILEPMMAVEVETP
                     EDYAGTVMGDLSSRRGMVQGMDEIAGGGGKIIKAEVPLSEMFGYSTSLRSATQGRATY
                     SMEFKHYSEAPKNVIDAIVTLKAK"
BASE COUNT          666 a          908 c          692 g          709 t
ORIGIN      
        1 cagcttcgac gccagctttg gcgtagtggc cggcagcagc tttcgtcaca cgcgaggcgc
       61 gacgctgacc gaatgcaacc tgaacagcgg aataaccatc cacttcaggc gttttgattt
      121 gggtcacgcg gttgttcgac acatccagca cggtgacagg aacagcatcc ccgccttcca
      181 tgaaaatacg catcatacca accttgcgac caacaaggcc caggctcatt gtttttctcc
      241 atacccgcct gcgattggac gggactgatg tttatacagc taaaattgtg gaatcacgaa
      301 catcatgact ccaccgaagc cggctagtat aaccggacaa atttctattt gcaatatcct
      361 acagtgtcgt ggtatgtcaa aatattaccg tccgtagctc ttgccacaat gtttattgca
      421 gcttgatttc tacatcgacg ccagccggca gatccagctt catcaatgca tcgacagtct
      481 tgtcggtcgg atcaacgata tccatcaggc gctggtgcgt acgaatttcg aattgatcgc
      541 gcgaagtttt attgacgtgc ggagaacgca aaacatcgaa acgctggatg cgtgtcggca
      601 gcggaacagg gcctttgaca acagcaccgg tacgctttgc cgtatcgacg atttccagtg
      661 ccgactggtc gatcagacga tagtcgaatg ccttcaggcg gatacggatt ttctgactgg
      721 gaactgacat ggttttcctt taaagaacga accgtggatg actgtcatcc acggcaatga
      781 tcaatactgt tattgatgct tagtcgaaaa ttttggcgac gacacctgcg ccgacggtac
      841 gaccgccttc gcgaatggcg aagcgcaggc cttcttccat ggcgatcggg ttgatcagtt
      901 gcacagtgat cgacacattg tcgcccggca tgaccatctc cttgtccttc ggtaactcga
      961 tcgagccagt cacgtctgtc gtgcggaagt agaactgcgg acgataattg ttgaagaatg
     1021 gcgtatgacg ccctccctca tctttcgaca gcacatagat ctcgccggtg aagtgcttgt
     1081 gcggcttgat cgaacccggc ttggccagca cctgaccacg ctctacgtct tcacgcttcg
     1141 tgccacgcag cagcacgcca acgttgtccc ctgcctgacc ttgatccagc agcttgcgga
     1201 acatttccac gccggtacag gtcgtctttt gcgtctcacg gataccaaca atttcgattt
     1261 cttctcctac cttgatgaca ccgcgctcta cacgtcccgt gacgaccgta ccccggccag
     1321 aaatcgagaa cacgtcttcc actggcatca gaaagctgcc gtccactgcc cgttccggtg
     1381 tcggtatata actgtccaac gcttcggcca acgccatgat cgcctgttcc cccagcggtc
     1441 cagtatcgcc ttccagcgcc agtttcgcag aacccttgat gatcggcagg tcatctcccg
     1501 gaaattcata cttcgacagc agttcgcgta cttccatttc aaccagttcc agcagttcct
     1561 cgtcatccac catgtccgcc ttgttcagaa acacgatgat gtaaggaaca cccacctggc
     1621 gcgacagcag aatgtgttca cgtgtctgcg gcatcggacc gtctgcagcc gagcacacca
     1681 gaatcgcgcc gtccatctgc gccgctcccg taatcatgtt cttcacatag tcggcgtgcc
     1741 ccggacagtc aacgtgcgca taatgccggt tcgctgtctc atattcaacg tgtgccgtat
     1801 tgatcgtgat gccacgcgcc ttctcttccg gcgccgcatc aatctgatcg taagccttcg
     1861 cctcccctcc aaacttcttc gacagcaccg tcgctatcgc cgccgtcagc gtcgtcttcc
     1921 catggtcaac gtgacctatc gtacccacat tcacgtgcgg cttcgtccgc tcaaacttgc
     1981 cttttgccat cttgcatttc ctttaaaaag aacgattttc tcaacagtta agaacctgcc
     2041 cctgccaatg cagggagcgg gtaaaatctg gattacttgg ccttcaacgt gacaatcgcg
     2101 tcaatgacat tcttcggcgc ttcagaatag tgcttgaatt ccatcgaata agtcgcacga
     2161 ccttgtgttg ccgaacgcaa cgaagtcgag taaccgaaca tttccgacaa tggcacctct
     2221 gccttgatga tcttgccgcc accgccggcg atttcatcca tgccctgcac cataccgcga
     2281 cgggacgaca aatcacccat tacagtaccg gcgtagtctt ccggcgtttc aacttcgaca
     2341 gccatcattg gctccagaat gaccggactg gctttacggc aaccatcctt gaacgccatc
     2401 gaagcggcca tgcggaacgc attttcgttc gagtcaacat catggtagga accgaagaac
     2461 aaagtgacct tgacatcaac caccgggtaa ccagccagca cgccggtgtt cagtgtttcg
     2521 cgcacaccct tttcaactgc cgggatgaat tcgcgaggaa cggtaccgcc cttgatcgca
     2581 tcgacgaatt cgaaaccttt gcctggctcc tgcggttcaa tcttcaacac cacgtgaccg
     2641 tactgaccac gaccaccgga ttgcttgacg aatttgcctt cgatttcttc gcaagtcttg
     2701 cgaatagttt cgcggtaagc tacctgcggc ttaccgaccg tcgcttccac gttgaattca
     2761 cgcttcatac gatcgacgat gatgtccaga tgcagctcgc ccataccggc aatgatcgtt
     2821 tggcccgatt cttcgtcggt acgcacgcga aacgatggat cttccgcagc tagacgattc
     2881 aacgccagcc ccatcttttc ctggtcagcc ttggttttcg gctcaacagc ctgcgaaatc
     2941 accggctcag ggaaaaccat gcgctccagc acgac
//
LOCUS       DASRDH010000300         3453 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1054942,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000300 DASRDH010000000
VERSION     DASRDH010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 3453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3453
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1054942"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(202..711)
                     /gene="lspA"
                     /locus_tag="VFA14_06945"
     CDS             complement(202..711)
                     /gene="lspA"
                     /locus_tag="VFA14_06945"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007880235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HZS81145.1"
                     /translation="MASNKKNLMRNPNNHSSLLPWLGISLIVLLLDQLSKITITKLFD
                     VGQSYPVTDFFNLVLVYNKGAAFSFLATESGWQRHLFTAIGIAASVYIVHLLRKHARQ
                     RMFCWALALILGGAIGNVIDRILYGHVIDFLDIFIGNWHWPAFNIADSAICIGAILFV
                     QDELRRVDR"
     gene            complement(728..>3453)
                     /gene="ileS"
                     /locus_tag="VFA14_06950"
     CDS             complement(728..>3453)
                     /gene="ileS"
                     /locus_tag="VFA14_06950"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013233423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HZS81146.1"
                     /translation="IRAASKNRPKFILHDGPPYANGAIHLGHAVNKILKDMIVKARNM
                     AGFDAPYVPGWDCHGMPIEIQIEKKYGKNLPVAQVQARARAYANEQIALQKKDFMRLG
                     VLGEWDKPYKTMNFSNEADELRALGVILEKAYVYRGLKPVNWCFDCGSALAEAEVEYQ
                     DKRDPAIDVGFPFAEHDRVARAFGLPKLPTDKGYIVIWTTTPWTIPSNQALNVHPECD
                     YALVRTERNGEPLLLILANDLVASCLQRFGLEGEVIATTKGAALSQIAFRHPLAAADK
                     GYDRLSPIYPGDYVSADSGTGVVHSAPAYGIEDFTSCKAHGMQDDAILNPVMGDGRYA
                     SWLPLFAGLTIWEASKPICARLEETGALFKLVMLDHSYMHCWRHKTPTIYRATSQWFA
                     SMDNTPKDGGKSLRETALQGIEDTAFFPAWGKARLHGMIAGRPDWTLSRQRQWGVPMA
                     FFIHKETGLLHPRTPELLEHIAERIEQGGIEAWQTLDPKELLGDEADHYLKSKDTLDV
                     WFDSGSTHQTVLRGSHRQELQFPADLYLEGSDQHRGWFHSSLLSSSMLNGCAPYKALL
                     THGFVVDGEGRKMSKSLKNGIEPQTIVDSLGAEILRLWVASSDYSGEISISDEILKRV
                     TETYRRIRNTLRFLLANTSDFDPARHTVPVAGMLEIDRYAIASMANLQKDILAHYESY
                     EFHPAVSRLQTYCSEDLGGFYLDILKDRLYTSGVDSHARRSAQTAIWHITQALLRLMA
                     PMLSFTAEEAWRYFAGKEAFADSGETIFTQTYYALPEVAQADALLARFKELHAIRAAV
                     TRQLEEVRITGSIGSSLQAEVELKVSGSKVALLQSLGDDLKFVLITSSATVTPVATQA
                     DEAILVTPSAHQKCERCWHYRANVGMHAEHPSLCGRCVSNLFGNGECRHFA"
BASE COUNT          697 a          955 c         1045 g          756 t
ORIGIN      
        1 cccaacgata cgccgggcct ggaaaatctg atggaagcgt tcaagacgaa actgccgggc
       61 tacgacgacg ctgcgatcca tgcagcggtg atcaacgcca tgggggcgct ggaaggcagc
      121 ttcgacattt ggcacagacc ggtgtcttga ccaccttgga agcatccctt tcaaggcgtg
      181 agtgccggtc tactgctcac ctcatctgtc gacgcgacgc aattcgtcct gcacaaacaa
      241 tatcgcgcct atacagatcg cgctatcggc gatattgaat gccggccagt gccagttgcc
      301 gatgaaaatg tcgagaaaat caatcacatg accgtagagg atgcggtcga tcacgttgcc
      361 gatggcgccg cccaggatca gtgccagcgc ccagcaaaac atgcgttggc gggcgtgctt
      421 gcgcagcaga tggacgatat agaccgaggc ggcaatgccg atcgcggtga acaggtgacg
      481 ttgccatccc gattcagtcg ccagaaagct gaaggccgcc cctttgttat agaccagcac
      541 cagattgaag aagtcggtga caggatatga ctggccgaca tcgaacagtt tggtaatagt
      601 gattttggat aactggtcca gcagcagcac gatcagggaa atacccagcc atggcagcag
      661 gctgctgtga ttgttggggt tgcgcatgag gttttttttg ttggaggcca tgaggtgcaa
      721 gggtgagtca ggcgaaatga cgacactcgc cgttgccaaa caggttggat acgcaacggc
      781 cgcataagct cgggtgttcg gcatgcatgc cgacattggc acggtaatgc cagcaacgct
      841 cacacttttg atgcgcggac ggcgtgacga gaatggcttc atcggcttgc gtcgccactg
      901 gcgtgaccgt ggcggacgag gtaatcagta cgaacttgag gtcgtcgccc agactttgca
      961 acagcgccac cttgctgcca ctgaccttga gttcgacttc tgcctgtagc gacgaaccga
     1021 tgctgccggt gatgcggact tcttccagtt gcctggtcac ggccgcgcgt atggcatgca
     1081 gctccttgaa cctggcgagc agcgcatcgg cttgcgccac ttccggcagc gcgtaatagg
     1141 tctgcgtgaa aatggtctcg ccgctatcgg caaaggcttc cttacccgca aaatagcgcc
     1201 aggcttcttc tgccgtgaac gatagcatcg gcgccatcag acgcaacagc gcctgcgtga
     1261 tatgccagat ggcggtctgc gcggaacggc gcgcgtgcga atcgacgccg ctggtataca
     1321 gacgatcctt gaggatgtcg aggtagaaac cgccaaggtc ttccgagcag tatgtttgca
     1381 gcctggacac cgctggatgg aattcatagc tctcgtagtg cgcgagaata tctttttgca
     1441 gattggccat gctggcaatc gcgtagcggt cgatttccag catcccggcc accggcacgg
     1501 tatgtctggc cggatcgaag tcggatgtgt tggcaagcag gaagcgcagt gtattgcgga
     1561 tgcggcgata agtttccgtc acgcgcttca ggatttcatc ggagatcgaa atttcaccgg
     1621 aatagtcgct cgatgctacc catagacgca ggatttccgc gcccagcgag tcgacgatgg
     1681 tttgcggttc gataccgttc ttgagtgatt tcgacatttt gcggccttcg ccgtcgacca
     1741 cgaaaccatg cgtcagcaat gccttgtatg gcgcgcagcc gttgagcatt gaagaactca
     1801 gcaatgatga atggaaccag ccgcgatgct ggtctgagcc ttccagatac agatcagccg
     1861 ggaactgcag ttcctgcctg tgcgaaccgc gcagcacagt ctgatgggtt gagccggagt
     1921 cgaaccagac atccagcgta tccttgctct ttagatagtg atcggcttcg tcgccgagca
     1981 gttctttcgg gtccagcgtt tgccatgctt cgatgccgcc ctgttcgatg cgctcggcga
     2041 tgtgctccag cagctcgggc gtgcgcggat gtagcagacc ggtttcctta tggatgaaga
     2101 acgccatcgg caccccccac tgacgttgac gcgatagcgt ccagtcggga cgtccggcga
     2161 tcatgccgtg caggcgtgcc ttgccccagg ctgggaaaaa ggctgtgtct tcgatgcctt
     2221 gcagcgcggt ttcgcgcagg ctcttgccgc cgtctttcgg ggtattgtcc atgctcgcga
     2281 accattgtga ggtagcacgg tagatggtcg gcgttttatg gcgccagcaa tgcatgtagc
     2341 tgtggtcgag catgaccagc ttgaacagcg cgccggtttc ttccagtctg gcgcagattg
     2401 gcttggaggc ttcccagatg gtcaggccgg caaacagcgg cagccatgac gcatagcggc
     2461 cgtcgcccat gaccggattg aggatggcgt catcctgcat accgtgcgcc ttgcacgacg
     2521 taaagtcttc gataccgtag gccggcgccg agtggacgac gccggtgccg ctatcggcgc
     2581 tcacgtagtc gcccggatag atcggagaca ggcgatcgta acctttgtcg gcagcagcca
     2641 gcgggtggcg gaaagcgatc tgcgacagtg ctgcgccttt ggtggtggcg atgacttcgc
     2701 cttccagacc gaagcgttgc aggcaggatg ccaccagatc gttggccaga atcagcagca
     2761 gcggttcgcc gttgcgctcg gtccgtacca gcgcgtagtc gcattccgga tgcacattga
     2821 gcgcctggtt ggatggaatg gtccaggggg tggtggtcca gatgacgatg tagcccttgt
     2881 ctgtcggcag cttgggtagg ccgaaggcgc gcgcgaccct gtcgtgttcc gcaaacggaa
     2941 agccgacgtc gatggccgga tctcgtttgt cctggtattc cacttccgct tcggccaacg
     3001 ccgagccgca atcgaaacac cagttgactg gcttcagacc acgatagaca taagcttttt
     3061 ccagaatcac gccaagcgca cgcaactcat cggcttcgtt ggaaaaattc atggtcttgt
     3121 agggtttgtc ccattcgccc agcacgccca gacgcatgaa atccttcttt tgtaatgcaa
     3181 tctgctcgtt ggcataagca cgcgccctgg cttgcacttg cgccactggc agatttttgc
     3241 cgtatttttt ttcgatctgg atttcgatgg gcatgccgtg gcaatcccag cccggcacat
     3301 agggcgcgtc gaagccggcc atgttgcgtg ccttgacgat catgtccttg aggattttgt
     3361 tgacagcgtg gcccagatgg attgcaccgt tggcatacgg tggaccgtcg tgcaggatga
     3421 acttcggacg gttcttcgat gctgcccgga tac
//
LOCUS       DASRDH010000301         4339 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_294060,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000301 DASRDH010000000
VERSION     DASRDH010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 4339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4339
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294060"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="VFA14_06955"
     CDS             complement(<1..341)
                     /locus_tag="VFA14_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZS81147.1"
                     /translation="MNASIASTTSSSLAGQATKAATARQDGCAKLLSWSLPVGLIVFW
                     EIAAQAGWLSSRILPEPLAVLRAAWNLAVTGELWRHLGVSAGRAASGFAVGSCLGLLL
                     GLLTGTLRQAE"
     gene            complement(351..1529)
                     /gene="ssuD"
                     /locus_tag="VFA14_06960"
     CDS             complement(351..1529)
                     /gene="ssuD"
                     /locus_tag="VFA14_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HZS81148.1"
                     /translation="MNVFWFIPTSGDSRYLGTAEGGRLVNHDYLKQVAVAADTLGYDG
                     VLLPTGRACEDAWVAAASLIGETRQLKFLIAVRPGLSAPTLSARMAATFDRLSGGRLL
                     INVVTGGDPSEQEGDGIFGSHQERYEISDEFLKIWREVLTKTHSGGETTFEGKHLRVN
                     KAKSLYPPIQNPYPPLYFGGSSDAAIDLAAEQVDVYLTWGEPPAAVAEKIAKVKARAE
                     QAGRTLRFGIRLHVIVRETTEAAWRTANELISKLDDETIEKAQQTFARMDSEGQRRMA
                     ALHGGKRDKLEVSPNLWAGIGLVRGGAGTALVGDGATVAARLKEYADLGIDTFILSGY
                     PHLEESYRFAELVFPLLPRKQKETLPGLNLAGPFGEIVSNSYAPPAKTRKEEAGVLTN
                     "
     gene            complement(1623..1748)
                     /locus_tag="VFA14_06965"
     CDS             complement(1623..1748)
                     /locus_tag="VFA14_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS81149.1"
                     /translation="MTISLTHRFFTLALAVALPIGIATAAAQTSGAPPTASLNWG"
     gene            complement(1771..2748)
                     /locus_tag="VFA14_06970"
     CDS             complement(1771..2748)
                     /locus_tag="VFA14_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007884489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HZS81150.1"
                     /translation="MHLQQCISRRRTFGLLSALTIAAASLSMLIMSNPADAQTRPVLR
                     IGYQKSASTLVLLKAHGALEKRLQARGVEVKWDQFAAGPQLLEALNVGSIDFGCVGET
                     PPIFAQAAGADFVYSFYEIPTPNAEGLLVPKNSPIKTVAELKGKKIAFNKGSDVHWLV
                     IALLKKAGLHYNDIQPVYLAPADARAAFEKGAIDAWAIWDPFQAAAEKQIGARRLATG
                     VGVVSHHEFFLSTRPYAEKNGDITRILLEEISKEGQWIRANIKDAAAQLALIQGLDAA
                     VIEIGLKRYEHIFKPADAQVLEEQQRIADAFFELKLIPKKIHVKDAVLK"
     gene            complement(2807..3346)
                     /gene="ssuE"
                     /locus_tag="VFA14_06975"
     CDS             complement(2807..3346)
                     /gene="ssuE"
                     /locus_tag="VFA14_06975"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0044273 - sulfur compound catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HZS81151.1"
                     /translation="MTILLLAGSPSALSRSTRLLHHVGEKLAALGHRYAKLDVRDLPG
                     QALIRADFNDAEIKAALARVEEASAVVIATPVYKAAYSGVLKAFLDLLPQFGLTGKLV
                     LPLATGGSQSHMLALDYALRPVLSSLGSRHILPSIYATEAQVAWSEQDSLVLDMSIVA
                     RVQEGVEQLSGSLLALQKT"
     gene            3594..>4339
                     /locus_tag="VFA14_06980"
     CDS             3594..>4339
                     /locus_tag="VFA14_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008327441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HZS81152.1"
                     /translation="MLKSALAAVTALFFVLAGVSPAQAGTSESTRKATVKKHVAKKTT
                     AKRKKSVKKIGHTHGKHRIVHQRVAASAAAPVMTAGDMAGLNLTHDPLALNSNVVLVI
                     DQGSLQVLFEKNPAVALPIASLTKLMTSLVVVEARQNMEEILTVTDDDVDREKHSGSR
                     LHVGAQLSRDDMLHIALMSSENRAASALGRNYPGGLPAFVAAMNAKARSLGMTDTRYV
                     DSTGLSSHNVASARDLAKLAVAAYKHPIIR"
BASE COUNT          910 a         1248 c         1232 g          949 t
ORIGIN      
        1 atttccgcct ggcgcaacgt gccggtcagc aagcccagca gcaggccaag gcagctgccg
       61 acagcgaaac cggatgcggc gcgtccggcg ctcacgccca gatgccgcca caactcgccc
      121 gttacagcca gattccaggc ggcgcgcaag acggccagcg gctccggcag aatgcggctg
      181 gataaccagc cggcctgcgc ggcaatttcc cagaacacga tcagtccgac cggcaaggac
      241 cacgacagca atttggcgca accgtcctgc ctggctgtgg cagccttggt agcttgaccg
      301 gccaacgatg atgatgtcgt tgatgcaatg cttgcgttca tgatgattcc tcagttcgtc
      361 aagacgcctg cttcttcctt cctggtcttc gccggcgggg cgtagctgtt gctgacgatt
      421 tcgccgaacg ggccggcaag attcaagcct ggcagcgttt ccttttgctt gcgcggcagc
      481 agcgggaaca ccagttcggc gaaacgatac gactcttcca gatgtggata gccggacagg
      541 atgaaggtat cgatgcccag gtcggcatat tccttcaagc gcgcagccac ggtcgctcca
      601 tcaccgacta acgcggtccc cgcgccgccg cgcaccaggc cgatgccggc ccagagattc
      661 ggactgactt ccagcttgtc gcgcttgccg ccgtgtagcg ccgccatgcg gcgctgccct
      721 tctgaatcca tcctggcgaa agtctgctgt gccttttcta tggtctcgtc atcgagcttg
      781 ctgatcagtt cattggcggt gcgccaggcg gcttcggtgg tttcgcgcac gatgacatgc
      841 aggcgaatgc caaaacgtaa tgtccgtcct gcttgttcag cacgcgcctt gaccttggcg
      901 attttctcgg cgacggcagc cggcggttcg ccccaggtca ggtagacgtc gacctgttcc
      961 gccgcgaggt cgatcgccgc gtcggaggag ccgccgaaat acagcggcgg atacggattc
     1021 tgaatcggtg gatacaggct cttggcttta ttgacgcgca aatgtttgcc ttcaaaggtg
     1081 gtttcgccac cgctgtgtgt tttggtcagc acttcgcgcc agatcttgag aaactcgtcg
     1141 gagatttcgt agcgttcctg atggctgccg aaaataccgt cgccctcctg ctcggacgga
     1201 tcgccgccgg tgacgacgtt gatcagcaga cgtccgccgg acaaacggtc gaaggtcgcc
     1261 gccatccggg ccgacagggt cggtgcgctc aggcccggac gcactgcaat cagaaatttc
     1321 agttgtctgg tttcaccgat cagactggcg gcggcgaccc aggcatcttc acaggcacgt
     1381 ccggtcggca gcagcacgcc gtcgtatccc aacgtatcgg ctgccaccgc gacctgcttc
     1441 aggtaatcat ggttgaccag acggccgcct tccgcggtgc caagataacg gctgtcgcca
     1501 gaggtgggaa taaaccagaa aacgttcatc tgcaactcca tagatagtgg tgaaggataa
     1561 tggctaagct tatttgtgcg tcggctgcca gacgatgctg gccatgttga tgcggcgtgg
     1621 gatcaacccc agtttaaaga agctgttggc ggcgcgcccg aggtctgcgc cgccgccgtc
     1681 gcgatgccga tgggcagcgc gacggccagt gcgagggtaa agaaacggtg agtcagagag
     1741 attgtcatga tagttttttg agagcgatac ttacttcaac acggcgtctt tgacatggat
     1801 cttctttgga atcagcttga gttcaaaaaa ggcatcggcg atgcgttgct gttcttccag
     1861 cacctgggcg tcggccggct tgaagatgtg ttcatagcgt ttcaggccga tttcgatgac
     1921 agctgcatca agtccttgaa tcagcgccag ctgcgctgcc gcgtccttga tgttggcacg
     1981 gatccactga ccttctttcg agatttcttc cagcagaatc ctggtgatgt caccattctt
     2041 ttccgcatag ggacgggtac tcaggaagaa ttcgtgatga ctgaccacgc cgacgccggt
     2101 cgccagacgg cgtgcgccga tctgtttctc ggcggcggcc tggaacggat cccagatggc
     2161 ccaggcgtcg attgcacctt tttcaaaggc ggcgcgtgca tcggcaggcg ccagatacac
     2221 cggctggata tcgttgtaat gcagacccgc ttttttgagc agggcaatga ccagccaatg
     2281 cacatcggaa cctttgttga aggcgatttt cttgcccttc agttcggcaa cggtcttgat
     2341 cggtgaattt tttggcacca gcaagccttc ggcgttgggt gtcggaattt cgtagaagct
     2401 atagacgaaa tccgcgcctg ccgcctgagc gaatatcggc ggcgtctctc ccacgcaacc
     2461 gaaatcgatc gaaccgacat ttagcgcctc cagcaattgt gggccggcag cgaattgatc
     2521 ccatttcacc tctactcctc gtgcctgaag acgcttttcc aatgcaccat gcgccttcag
     2581 caacaccagc gtgctggcgg atttctggta gccgatgcgc aatacaggtc tggtttgcgc
     2641 atcggcaggg ttgctcataa tcaacatact caagcttgcc gcagcgatag tgagcgcgga
     2701 cagtaaacca aaggtgcggc gtctggagat gcattgttgt agatgcataa tgttttccac
     2761 attgtttttt ggcgtaatga agtctctcca tacatgacgc ggattgtcat gtcttttgca
     2821 gagccagcaa gctgcctgac aactgctcga cgccttcctg cacgcgcgcc acgatggaca
     2881 tgtcgagcac cagactatcc tgctcggacc aggctacctg cgcttcagtc gcatagatgc
     2941 ttggcaagat atgcctggaa cccagtgatg acagcacggg acgcaatgcg taatccagcg
     3001 ccagcatgtg cgactggctg ccgccggttg ccagcggcaa caccagtttg ccggtgaggc
     3061 cgaattgcgg caataaatcg agaaaggctt tcagcacgcc gctgtaggcg gccttgtaga
     3121 ctggcgtcgc gatgacgacg gccgatgctt cttcgacacg cgccagcgcc gccttgattt
     3181 cggcgtcgtt gaaatcggca cggatcagcg cctgccccgg caaatcgcgt acatccagtt
     3241 tggcgtagcg atgtcccagt gccgccagct tttcaccgac gtgatgcagc aagcgcgtag
     3301 aacgcgatag cgcggaagga ctgccggcca gcaacagaat cgtcatgata aaagctcatc
     3361 cttctcggtt cagtttcgtt ttggaaacag cagaataaag agcgagcaaa aaaaaacgaa
     3421 cgaatatttt cttctctcaa tatgcggaaa tcagatatgt atcgagatta gtgcttggag
     3481 cctgttcccg gtgagaagat cgggaacagg ctccaagtta tcttgcgatt ttctcttagt
     3541 ttgctacact gccaattagt tacttgtatg tgccaatcgg ggagagggta tcaatgctta
     3601 aatctgcgtt agccgctgtt accgcgctgt tttttgtact cgccggcgtt tctcctgcgc
     3661 aagccggaac gtcagaaagt accaggaaag ccacagtcaa aaagcacgtc gccaaaaaaa
     3721 ccaccgccaa acgtaaaaag tcagtcaaga aaatcggcca cacacatggc aagcaccgca
     3781 tcgtccatca gcgcgtcgct gcgtctgcgg cggcgccggt gatgactgcc ggtgacatgg
     3841 ccggcctgaa tctgacgcat gatcctttgg ccttgaactc caatgtcgtg ctggtgatag
     3901 accagggcag tttacaggtg ttattcgaaa aaaatcctgc ggtggcattg ccgattgctt
     3961 ccctcaccaa actgatgaca tcgctggtcg tggtcgaagc gcgtcagaac atggaagaaa
     4021 tcctgaccgt caccgatgac gatgtcgacc gcgaaaaaca cagcggatcc cgtctgcatg
     4081 tgggcgcgca attatcgcgt gacgatatgc tgcacattgc cctgatgagt tcagaaaacc
     4141 gcgctgcttc ggcgcttggt cgcaattatc cgggcggctt gccggctttt gtcgccgcca
     4201 tgaacgccaa ggcgcgctcg ctgggcatga cggatacgcg ctatgtcgac tcgaccggtc
     4261 tctccagcca taatgttgcc agcgcgcgtg atctcgccaa gctggcggtc gctgcctaca
     4321 aacacccaat catccgcca
//
LOCUS       DASRDH010000302         3333 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_634536,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000302 DASRDH010000000
VERSION     DASRDH010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 3333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3333
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_634536"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="VFA14_06985"
     CDS             complement(<1..206)
                     /locus_tag="VFA14_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HZS81153.1"
                     /translation="MSSFFQKRHEQAWLKLGFADDAGTTRMITRSHCGPLRVQKPLYP
                     EHPAVCHAIIVHPPGGILGGDQLS"
     gene            complement(231..1982)
                     /gene="lpdA"
                     /locus_tag="VFA14_06990"
     CDS             complement(231..1982)
                     /gene="lpdA"
                     /locus_tag="VFA14_06990"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HZS81154.1"
                     /translation="MNLSAVKVPDIGDFKEVEVIELLVKVGDLIKVEQSLITVESDKA
                     SMEIPSSAAGVVKELKVKVGDRVAEGSVLLLVEAVAGGTATAPAAAPAAAPAPVAVGA
                     GGSAGIECDMMVLGAGPGGYSAAFRSADLGMSTVLIERYPTLGGVCLNVGCIPSKALL
                     HVAAVIDESAAMAAHGVTFGKPQIDIDRLRSYKEKVISTMTDGLSGMARARKVSVVQG
                     VGQFIGANHIEVTAADGSRKTVKFRQAIIAAGSATVDLPFVQAREKADPRIVDSTGAL
                     ELRQLPRRMLVIGGGIIGLEMATVYSTLGARIDIVEMMDGLMQGADRDMVKVWQKFNE
                     KRFDKILLKTRTVAVETLKEGIKVSFEASEAGVRAPEPQLYDLVLVAVGRSPNGKKIA
                     ADKAGVIVTDRGFIPVDSQMRTNVPHIFAIGDLVGQPMLAHKAVHEAHVAAEAAAGQK
                     SYFDASVIPSVAYTDPEVAWVGITEDEAAAKGIRLEQGRFPWAASGRAVANGRAEGFT
                     KLLFDAETHRIVGGGIVGTHAGDMIGEIALAIEMGADAIDIGKTIHPHPTLGESIGMA
                     AEVCKGVCTDLPPARRK"
     gene            complement(2009..3295)
                     /gene="aceF"
                     /locus_tag="VFA14_06995"
     CDS             complement(2009..3295)
                     /gene="aceF"
                     /locus_tag="VFA14_06995"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HZS81155.1"
                     /translation="MSQIEVKIPDIGDFKEVEVIEILVKTGDIIKVEQSLLTVESDKA
                     SMEIPSSHAGVVKEIKIKLGDKIAEGSVVAIVDAVDAAAPAVSAPAAAVVPAPAAPAP
                     AAVPAAASAMTVAAVAPGVGSKSHASPSVRKFARELGVDLTRVVGTGPKRRILREDIQ
                     EFVKAALAGDAAPAKNGSGAGLDLLPWPSLDFSKFGPTELQAMSRIRKLSGPNLHRNW
                     VMIPHVTQFDEADVTGLEEFRKTSNDAYAKARSPVKLTILAFVIKASVAVLKKFPAFN
                     SSIDAKGENLILKQYYNIGFAADTPNGLVVPVVKNADQKSIAQIATEISELSAQARDG
                     KLKPVDMQGATFTISSLGGIGGTAFTPIINAPEVAILGLSKAIIKPVWDGKQFAPRLM
                     MPLSLSFDHRVIDGAMGARFSACLADMLGDLRKSLL"
BASE COUNT          641 a         1025 c          954 g          713 t
ORIGIN      
        1 atgctcaatt gatcgccacc gagtataccg cccggcggat gcacgatgat agcatggcag
       61 acggcgggat gttcaggata tagcggcttt tgcacgcgca gtggaccgca gtggctgcgc
      121 gttatcatgc gcgtggtgcc ggcatcgtcg gcaaagccca gcttcagcca agcctgttca
      181 tggcgtttct ggaagaaact cgacatcgca gaaacgacag agatcagaaa ttatttcctg
      241 cgtgccggcg gcaggtcggt gcaaacgccc ttgcagactt ccgccgccat gccgatcgat
      301 tcacccagtg tcgggtgtgg atggatggtc ttgccaatat cgatggcgtc ggcacccatt
      361 tcgatagcca gtgcgatttc accgatcatg tcgccggcgt gcgtgccgac gataccgccg
      421 ccgacgatgc gatgcgtttc ggcatcgaac agcagcttgg tgaaaccttc ggcacggccg
      481 ttggcgacag cgcgaccgga agcagcccac gggaagcggc cctgctccag cctgatacct
      541 ttggccgcgg cttcgtcttc ggtgatgccg acccatgcca cttccggatc ggtgtaggcc
      601 accgatggaa tgacgctggc gtcgaaatag ctcttctgcc ctgccgcggc ttccgcggcg
      661 acgtgcgctt catgcacggc cttgtgcgcc agcatcggct gaccaaccag atcgccgatg
      721 gcgaagatgt gcggtacgtt ggtgcgcatc tggctatcaa caggaatgaa accgcgatcg
      781 gtcacgatca caccggcttt gtcggcggcg atcttcttgc cgttcgggct gcggccgacg
      841 gcgaccagca ccagatcata aagttgcggt tccggcgccc tgacgccggc ttcgctagct
      901 tcaaagctga ctttgatgcc ttccttcaag gtttcgacgg cgaccgtcct ggtcttgagc
      961 aggatcttgt cgaaacgctt ttcgttgaat ttttgccaga ccttgaccat gtcacggtca
     1021 gcaccctgca tcaggccgtc catcatttcg acgatgtcga tgcgcgctcc cagcgtcgaa
     1081 tacacggtcg ccatttccag gccgatgatg ccgccgccaa tgaccagcat acgtctgggc
     1141 agttgacgca gctccagcgc gccggtcgaa tcgacgatgc gcggatccgc cttttccctg
     1201 gcttgtacga atggcaggtc caccgtcgcc gaaccggcgg cgatgatggc ttgcctgaat
     1261 ttgacggtct tcctgctgcc atcggcggcg gtgacttcga tatggttggc gccgatgaac
     1321 tgtccaacgc cttggacgac actgaccttg cgcgccctag ccatgccgga taaaccgtca
     1381 gtcatggtgc tgatgacttt ttccttatag ctgcgcaacc tgtcgatgtc gatctgcggc
     1441 ttgccgaagg tgacgccatg cgccgccatg gcggcgcttt catcgatcac ggcagcgacg
     1501 tgcagcaacg ctttcgacgg gatgcaaccg acgttgaggc agacgccgcc gagggtcgga
     1561 tagcgctcaa tcagcaccgt gctcatgccc agatcggccg aacggaaggc ggcggaataa
     1621 ccgccgggac cggcgcccag caccatcatg tcacattcga tgccagctga gccgccagca
     1681 ccgacagcaa ccggcgccgg ggcagcagca ggagccgcag ccggcgctgt tgccgtaccg
     1741 ccggcaacag cttccaccag cagcagcacg gagccttcgg caaccctgtc gccgaccttg
     1801 actttcaatt ccttgaccac acccgcagcg ctggagggaa tctccatgct ggctttgtcg
     1861 gactcgacgg tgatcagcga ctgttctacc ttgataaggt cgccaacctt gaccagcaac
     1921 tcgatgacct ccacttcctt gaagtcaccg atatctggaa ctttgactgc gctcaaattc
     1981 atatactcga ctccatatgt cctgtgaatt acagcagtga tttgcgcaaa tcgcccagca
     2041 tatcagccag acaggccgaa aagcgcgcgc ccatcgcgcc atcgatcaca cggtgatcaa
     2101 aagacagtga gagcggcatc atcagacgcg gcgcgaattg tttgccgtcc cagaccggtt
     2161 tgatgatcgc cttcgacaga cccaggatcg ccacctcagg cgcattgatg atcggcgtga
     2221 aagccgtgcc gccaatacct cccagtgatg aaatagtgaa agtcgcacct tgcatgtcaa
     2281 caggtttcaa tttgccgtcg cgcgcctgcg ctgacagttc gcttatttct gtggcgatct
     2341 gggctatgct cttctgatca gcgttcttga ccacaggcac caccaggcca ttcggtgtat
     2401 ccgccgcaaa gccgatgttg tagtactgtt tgaggatcag gttttcgccc ttggcatcga
     2461 tcgaggaatt aaaggccggg aatttcttca gcaccgcaac gctggccttg atcacaaacg
     2521 ccagtatggt cagcttgacc ggggatctgg ctttggcgta ggcgtcattg gacgtcttgc
     2581 ggaactcttc caatccagtc acatcggctt catcgaattg cgtcacgtgc gggatcatga
     2641 cccagttgcg gtgcagattc gggccgctga gcttcctgat gcgcgacatt gcctgtagct
     2701 cggtcgggcc gaacttgctg aaatccagcg acggccatgg caacagatcc agaccggcgc
     2761 cgctgccgtt cttcgctggc gcggcgtcac cggccagcgc tgccttgaca aactcctgga
     2821 tgtcttcccg caggatgcgc ctcttcggac cagtgccgac gacgcgggtc agatcgacgc
     2881 cgagttcgcg cgcgaacttg cgtaccgatg gcgaagcgtg cgatttgctg ccaacgcctg
     2941 gcgcaacagc agcgacggtc atcgcggatg ctgcggcggg aactgccgcc ggcgctggtg
     3001 cggccggcgc cggtacgaca gcggctgccg gagcagatac tgcaggcgct gccgcgtcta
     3061 cggcgtccac gatcgcaacc acagagcctt cggcaatctt gtcgccgagt ttgattttga
     3121 tttccttgac cacaccggcg tggctggagg ggatctccat gctggccttg tcggattcga
     3181 ccgtcagcag ggactgttcg actttgatga tatcgcctgt cttaaccagt atctcgatga
     3241 cttcgacttc cttgaaatcg ccaatgtccg gtatctttac ttcgatctga ctcatctctt
     3301 gtgctccatt agatttccaa catcactggg tca
//
LOCUS       DASRDH010000303         6342 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_948121,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000303 DASRDH010000000
VERSION     DASRDH010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 6342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6342
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_948121"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="VFA14_07000"
     CDS             complement(<1..273)
                     /locus_tag="VFA14_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS81156.1"
                     /translation="MNPGNGLRELHRVIAPPGSADDLAIVANELQELLKSDPVLKQPT
                     FVEPTIELLDIFAACKTEKTKQYEARKPLILAFSHELLRSIQSEYIH"
     gene            complement(662..1924)
                     /gene="pcaD"
                     /locus_tag="VFA14_07005"
     CDS             complement(662..1924)
                     /gene="pcaD"
                     /locus_tag="VFA14_07005"
                     /EC_number="3.1.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047570 - 3-oxoadipate enol-lactonase
                     activity [Evidence IEA];
                     GO_process: GO:0042952 - beta-ketoadipate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipate enol-lactonase"
                     /protein_id="HZS81157.1"
                     /translation="MSYDPLDHDFERGMRNRRSVLGDAWVDRSIGNATSFNAEFQNLI
                     TRFAWNEIWGRPGLDHKTRRIIVLAITIALGRWEEFELHVRAALLGNPATRLTPDEMK
                     EVLIQSAIYAGVPAANTAFTHAQQILREVGAQIGYTIAPLPPADTCHSGIGKEGRSSG
                     APALHYSVRVPRNGKAPRHTIVLSHAIGCDLMMWDTLANQLAADCKVIAYDHRGHGSS
                     EKTDGLYSMAALADDAAQMLRDLDTGPVIWIGLSLGGMVGQELALRHPALIRALVLSN
                     TTSGYPEAARQAWQQRIAIVREQGIEAIADAVMERYFHDRFRSEKTSTVTHFRKRLTS
                     TDPAAYVGCCNAVGSVDTTARLGRIDAPTLVIAGELDQGTPLSMAQTLADGIPGATLA
                     VIDGASHLSAIEQPEAFAELVTSFIRAL"
     gene            complement(1956..3329)
                     /locus_tag="VFA14_07010"
     CDS             complement(1956..3329)
                     /locus_tag="VFA14_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008332173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HZS81158.1"
                     /translation="MSVSLFDSFLTTPEMIAVFDDTSIVQAMLDFEAALAHAEAAEGV
                     IPEAAAHVIASVCKVQLYDIAAMIQAGRRAGTLAIPLVKELTGAVALHSQESAIHVHW
                     GSTSQDVLDTAMVLTTRTALKLLDDGLRELTTQLLALAAQHLSTPVLARTLMQPAQVI
                     SFGFKLTGWAAPLLRTRRQLRAQAEQALQLQLGGAVGTLAVLGDKGPAVAQRMADALG
                     LEVADAAWHTQRDTWVRLGLEVAVLTGSLGKLATDLSLMAQGELAELAEPSGNGRGAS
                     SAMPHKRNPVSSMIALAAAARAPQQAATLLATMGQQHERGLGNWQAELATWPSLFLGA
                     HGALKALNEAFSGLFVDASRMRRNIDALQGLVFAEAVSVYLAGVIGRPHAHALLEKLT
                     QQTITTQAQLLDVLTTAVKDDAQLSRQVDVQYLRTLFDPVQAAGVARVVAERQLHDLQ
                     IAAAHFD"
     gene            complement(3364..3882)
                     /locus_tag="VFA14_07015"
     CDS             complement(3364..3882)
                     /locus_tag="VFA14_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006461668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase"
                     /protein_id="HZS81159.1"
                     /translation="MSNKITTSQTIGPFPHEAWNWAVDLTAKVESSAATIVVKGAIFD
                     GDGIAIDDAWVETWMPDSAPVETAHAIPGYRRVPSNDEGGFKLQISMPQAATAGKPVA
                     YVTVFARGLTKHQFTAIFLEDDAGLAQSAILGQVPQQRRDTLIARKQADGSYLWDIHM
                     QGAQETVFFDYL"
     gene            complement(3898..4593)
                     /gene="pcaH"
                     /locus_tag="VFA14_07020"
     CDS             complement(3898..4593)
                     /gene="pcaH"
                     /locus_tag="VFA14_07020"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit beta"
                     /protein_id="HZS81160.1"
                     /translation="MYFDVVDSGVYPELIYPPYRSTVKRGPTQAPLRIKAQAATTTNL
                     FASPRLILQHDMDMTAQGRSEPLGEKIVVTGRVFDEDGKPVHNSLLEVWQCNAAGRYW
                     HKRDQHDAPLDPNFSGFGKMLTDDDGRYRFVTIKPGPYPWSNHYKAWRPAHIHFSLFG
                     NVYAQRLVTQMYFPGDPLFDYDPIFQSIPDVAARQRLISRFSMEQTVDDKMLGYEFDI
                     VLRGRNATPSMGI"
     gene            complement(4718..5665)
                     /locus_tag="VFA14_07025"
     CDS             complement(4718..5665)
                     /locus_tag="VFA14_07025"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007884269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HZS81161.1"
                     /translation="MNNKVKQIGRLRLLMLGCGNIGMRLLPLLRARFRVFVVTSNPAR
                     CAELRAAGGVPLVADLDQPATLGRLARLARVIVHLAPPQSSGDKDQRTRNLAAILPDR
                     ANLVYISTTGVYGDCDGARFDETRTTRPASPRAQRRLDAETVLRAWARRSGSRLAILR
                     VPGIYARDRLPLERLQKETPALRAEDDIYTNHIHADDLAMIVARAIYRARPLRVYHAV
                     DESEMKMGDYFDAVARSFGLPLPPRLPRHELQQQVSPMMLSFMAESRRLRNDRLRCEL
                     GVRLRYPDVATFLMALPEKREHSKSCYLCDDRPNRANTG"
     gene            5678..>6342
                     /locus_tag="VFA14_07030"
     CDS             5678..>6342
                     /locus_tag="VFA14_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HZS81162.1"
                     /translation="MSFQVTVQPGGHQFTCTEGETILAAAIRAGVSLPYGCRNGACGS
                     CKGRLLEGSVGHDPHQERALSAHDENLGFSLLCCARPHSDVVIEACEVIGAGDFPVKK
                     VPVRVVKLDKVADDVIVLSLQLPANDRLQYKAGQYLEFMLRDGKRRSYSMANAPHKDE
                     YLTLHIRHMPGGLFTDQVFSTLKERDILRFEGPLGSFFLREDSDKPIVLLASGTGFAP
                     IKA"
BASE COUNT         1282 a         1828 c         1914 g         1318 t
ORIGIN      
        1 atgaatgtac tcggattgaa ttgaccgcaa aagctcgtgg gaaaatgcca gaattagcgg
       61 cttacgtgct tcgtactgtt ttgtcttttc ggtcttgcag gcggcaaata tgtccagtaa
      121 ttcaatcgta ggctcaacaa acgtcggttg tttaaggaca gggtcgctct ttaacaattc
      181 ttgcaattca ttggcgacta tggcgaggtc gtcagcgctt cccggtgggg caatcacgcg
      241 gtgcaattcc cgtagtccgt tgcccggatt catggctctg actcgatacg aatccaagga
      301 gaacgcatgc accatgttgt accaacatga tgcaaaataa tattgctctg ctgtcagcgg
      361 aagtaagcca agtcggttgt ctatcctcat gcggcggttt tccttgattt ttttttatgg
      421 cgatagtaag ctagctcgtt agtttaattg taagccttga ttcagtcgag gtttgcacag
      481 tttacgaaag gggaatgggg gttcgggtct gggcgtgttc ccgctgtgct ttccaccccg
      541 cctatttgag cggcccgctt ttgcgggccg ttcccattat gtctaactct gatcaacgcc
      601 agtctgcatt cgatcatcag ttgtccatcc gcaaacaaaa gattttgaat agctttttag
      661 atcacaatgc acgaatgaag ctcgtcacca gttccgcaaa tgcctccggc tgttcgatgg
      721 cgctgaggtg cgaagcgcca tcgatgaccg ccagcgttgc gccgggaatg ccatcagcca
      781 gcgtctgtgc catcgataac ggcgttccct gatccagttc accggcaatc accagcgtcg
      841 gcgcatcgat ccggcccagg cgcgccgtgg tatcgacgct gccgacggcg ttgcagcagc
      901 cgacgtaagc ggcggggtcg gtgctggtca ggcgtttgcg aaagtgtgtc acggtagatg
      961 ttttttcaga gcggaagcga tcatggaagt agcgttccat caccgcatcg gcaatcgctt
     1021 cgatgccctg ttcgcgaacg atggcgatgc gctgctgcca tgcctgccgc gcagcttccg
     1081 gatagccgga ggtggtattg gacagtacca gcgcacgtat cagcgcgggg tgacgcaggg
     1141 ccagttcctg gccgaccatg ccgcccaggg acaaaccgat ccagatcacc gggccggtat
     1201 cgagatcgcg cagcatctgt gcggcatcgt ccgccagcgc agccatgcta tacagaccat
     1261 cggttttttc cgaactgccg tggccgcgat ggtcataggc gatgaccttg caatcggcgg
     1321 ccagttgatt ggccagcgta tcccacatca tcaggtcgca gccgatagca tggctgagta
     1381 cgatggtatg gcgcggcgcc ttgccgttgc gcggtacacg cacactatag tgcagcgcgg
     1441 gcgcgccgct gctgcggccc tctttgccga taccggaatg gcaggtgtcg gccggcggca
     1501 gcggcgcgat ggtatagccg atctgcgcgc caacttcgcg caggatttgc tgggcatggg
     1561 taaacgccgt attggcggcc ggcacgccgg cataaatcgc cgactggatc agtacttcct
     1621 tcatttcatc cggcgtcagg cgcgtggcgg gattgcccag cagagccgcg cgcacatgca
     1681 gttcgaactc ttcccagcga cccagcgcga tggtgatcgc cagcacgatg atacggcgcg
     1741 tcttgtggtc gaggccgggg cggccccaga tctcattcca ggcgaagcgg gtgatcaggt
     1801 tctggaattc cgcattgaaa ctggtggcat tgcctatcga gcggtctacc caggcatcgc
     1861 ccaggacgct gcggcgattg cgcatgccgc gttcaaaatc gtgatcgagg gggtcgtaac
     1921 tcatgtttgt ctcgcgtaat cctgaaggga aaacatcaat cgaaatgggc ggcggcgatc
     1981 tgcaagtcgt gcaactgtcg ttctgccacc acgcgcgcca cgccggctgc ctggactgga
     2041 tcgaacagcg tccgcagata ctgcacgtcg acctgccggc tgagttgggc gtcatccttg
     2101 acggcggtgg tgagtacatc gagcagttgg gcttgggtag tgatggtttg ctgcgtcagt
     2161 ttttccagca acgcatgggc gtgcggccgg ccaatgacgc cggccagata aactgatact
     2221 gcttcggcaa ataccagccc ttgcaacgca tcgatgttgc ggcgcatacg cgacgcgtcg
     2281 acaaacaggc cggaaaaagc ttcattcaag gccttgagcg caccatgcgc gccgaggaat
     2341 aacgatggcc acgtcgccag ctccgcctgc cagttaccga gtccgcgttc atgctgctgt
     2401 cccattgtcg ccagcaaggt cgcggcctgt tgcggcgcac gcgcggctgc cgccaacgcg
     2461 atcatcgacg ataccgggtt acgtttgtgt ggcattgccg acgaagcgcc gcggccgttg
     2521 ccggagggtt cggccagctc ggcgagttcg ccctgcgcca tcaggctgag gtcggttgcc
     2581 agcttgccga gactgccggt caaaacggcg acttccaggc ccagccggac ccatgtatcg
     2641 cgttgggtat gccaggcggc atcggctacc tcgaggccga gcgcatcagc catgcgttgc
     2701 gccacggcag gccccttgtc gccgagtacc gccagcgtgc cgacggcacc gcccagttgc
     2761 agttgcagcg cctgttcagc ctgcgcgcgc aattgccggc gcgtgcgcaa cagtggcgcg
     2821 gcccagccgg tcagcttgaa accaaagctg atgacttgcg ctggctgcat cagcgtgcgt
     2881 gccagcaccg gcgttgataa atgttgcgcg gccagcgcca gcagctgcgt cgtcagttcg
     2941 cgcaagccat catcgagcag cttcagcgcc gtgcgcgtag tcagcaccat cgctgtatcg
     3001 agtacgtcct ggctggtgct gccccaatgc acgtggatgg ccgattcctg gctgtggagc
     3061 gcgactgccc cggtcagctc cttgaccagc ggaatcgcca gtgtaccggc acggcggccg
     3121 gcctggatca tggcggcaat atcataaagc tggaccttgc agacactggc gatgacatgt
     3181 gctgcagctt cggggatgac accctcggcg gcttcggcat gggccagcgc tgcttcgaag
     3241 tcgagcatcg cctgcacgat ggaggtatcg tcgaagaccg cgatcatctc cggcgtggtc
     3301 aaaaaactgt cgaataggga aacgctcact agaactccat cacgaaaaaa tctgaccgga
     3361 cgttcagagg taatcaaaaa ataccgtttc ctgagcgccc tgcatatgaa tgtcccacaa
     3421 ataggagccg tccgcttgct tgcgcgcgat cagcgtgtca cggcgctgct gcggaacttg
     3481 tccgagaatc gccgactgcg ccagcccggc atcgtcttcc agaaagatgg ccgtgaactg
     3541 gtgcttggtc agcccgcgcg cgaacacggt gacataagcc accggtttgc cggcagttgc
     3601 cgcctgcggc atgctgatct gcaacttgaa accgccttcg tcattgctcg gcacgcggcg
     3661 gtaaccgggg atggcatgcg cggtttcgac cggcgcgctg tcgggcatcc aggtttctac
     3721 ccaggcatca tcgatggcga taccgtcgcc atcgaaaatg gcacccttga caacgatcgt
     3781 cgcagcgctg ctctcgacct tggcagtcag gtccactgcc cagttccatg cttcgtgcgg
     3841 gaacgggccg atggtttgcg atgtggtgat tttattgctc atgccgtctc ctgcatctca
     3901 gattcccatg gagggggtag cgttacgccc gcgcagaacg atatcaaatt cgtaaccgag
     3961 catcttgtca tcgacagtct gctccatgct gaagcgggaa atcaggcgct ggcgtgcggc
     4021 aacatcgggg atgctctgaa agatagggtc gtagtcgaac agcggatcgc cgggaaaata
     4081 catctgcgtc accaggcgtt gcgcatagac gttgccgaac agcgaaaagt ggatgtgcgc
     4141 aggacgccat gccttgtagt gattactcca tggataaggt cccggcttga tcgtgacgaa
     4201 acgataacga ccgtcatcat cggtgagcat tttaccgaag ccggaaaagt tggggtcgag
     4261 cggcgcatca tgctggtcgc gcttatgcca gtagcgaccg gctgcgttgc attgccagac
     4321 ttccagcagc gaattatgca ccggtttgcc atcttcgtcg aacacgcggc cggtgaccac
     4381 gattttttca ccgagcggct cgctcctgcc ttgtgctgtc atgtccatat catgctgcag
     4441 aatcagcctg ggcgaggcga acagattggt ggtggttgct gcctgcgcct tgatacgcaa
     4501 tggcgcctgc gtcggtccgc gcttgacggt ggatcgatag ggcgggtaga tcaattccgg
     4561 ataaacgccg gagtcgacga cgtcgaagta catggtgtct ccttcaagat ggatggtaaa
     4621 agcagcatca cattgcagca aagatgtgtg cattattttt attctgcggc gatattaagc
     4681 aggcaaggca gctgccacaa gagtgcggat ggaagattta tccggtattc gctctattcg
     4741 ggcgatcatc gcacaaataa caggatttag aatgctctcg cttttccggt aacgccatca
     4801 gaaaagtcgc tacatccgga tagcgcaggc gcacgccgag ttcacagcgc agacggtcgt
     4861 tgcgcaagcg gcgcgattcc gccatgaagg acagcatcat cggagatacc tgctgctgca
     4921 gttcgtgacg cggcaggcgc ggcggcagcg gcaagccgaa actcctggcg acggcgtcga
     4981 agtaatcgcc cattttcatc tcagattcat caacggcgtg gtacacgcgc aatggccgcg
     5041 cgcgatagat cgcccgcgcg acgatcatgg ccaggtcgtc ggcatggatg tgattggtat
     5101 agatgtcatc ctcggcgcgc aaggccggcg tctccttttg caagcgctcc agcggcagac
     5161 ggtcgcgcgc gtaaataccc ggcacgcgca aaattgccaa gcggctaccg gaacgccgtg
     5221 cccaggcgcg taagacagtt tccgcgtcca agcggcgctg cgcgcgcggg ctggccggtc
     5281 tggtggtacg cgtttcatcg aaacgggcgc cgtcgcaatc gccatagaca ccggtggtac
     5341 tgatgtaaac gagattggcg cggtcgggta aaatggcggc cagattgcgg gtgcgctggt
     5401 ctttgtcgcc ggaagattgt ggcggagcaa gatgcacgat gacccgcgcc aatcgcgcca
     5461 gtcggcctag cgtagcaggt tgatcgagat cggcgaccag tggaacccca ccggccgcac
     5521 gcaattccgc gcagcgcgcc ggattgctgg taacaacgaa tacacggaag cgcgcacgca
     5581 gcaacggcag caagcgcatg ccgatgttgc cacaacccag catcaggaga cgcagccggc
     5641 cgatttgttt tactttatta ttcatggtca ggattgtatg agttttcaag ttaccgttca
     5701 gcccggcggt catcaattta cctgcactga aggcgaaacc attctcgccg cggcaatccg
     5761 cgccggcgtc agtctgcctt atggctgcag gaatggcgca tgcggttcat gcaaaggcag
     5821 actacttgaa ggcagcgtcg gccacgaccc acatcaggaa cgcgctttgt cggcacatga
     5881 tgaaaatctg ggtttttcac tgctctgctg cgccagaccg cattcggatg tggtgatcga
     5941 agcgtgtgaa gtaatcggtg ccggcgattt tccggtcaaa aaagtaccgg tgcgtgtggt
     6001 caagctggac aaggtcgccg acgatgtcat cgtcctgtcg ctgcaactgc cggccaacga
     6061 ccggctgcaa tacaaggccg gccagtacct cgagttcatg ctgcgcgacg gtaagcgccg
     6121 cagctacagc atggccaacg cgccgcacaa ggatgagtac ctgacactgc acattcgcca
     6181 catgccaggc ggtttgttca ccgaccaggt attttcaaca ctcaaggaac gtgacatctt
     6241 gcgcttcgaa ggtccgctgg gcagcttctt cctgcgcgaa gacagcgaca aaccgatcgt
     6301 gctgctggca tccggcacag gcttcgcgcc gatcaaggcc at
//
LOCUS       DASRDH010000304         3384 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1447042,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000304 DASRDH010000000
VERSION     DASRDH010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 3384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3384
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1447042"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /locus_tag="VFA14_07035"
     CDS             <1..368
                     /locus_tag="VFA14_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007880231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase M48"
                     /protein_id="HZS81163.1"
                     /translation="RQRMTATIGSMIDKLIRTQPDATITAGTGGIRPYPQELDAAADN
                     LALHMLVRAGYSIDNYAAFWQRLATQYPASVRNAYTALHPATAYRMTVINEAVRTLKT
                     GKTTENRKRPATPPVQGQV"
     gene            complement(431..688)
                     /locus_tag="VFA14_07040"
     CDS             complement(431..688)
                     /locus_tag="VFA14_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006713246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3567 domain-containing protein"
                     /protein_id="HZS81164.1"
                     /translation="MNLIYNSEQYSVLEFGVDWNHEAMRFGGYEITNKPGRREIYIDG
                     PAAELFRRDVQDLIATAPTMEDIDTFLSRYDLMMRYSLALH"
     gene            complement(821..1561)
                     /locus_tag="VFA14_07045"
     CDS             complement(821..1561)
                     /locus_tag="VFA14_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016836073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZS81165.1"
                     /translation="MANILVVDDEMGIRELLSEILGDEGHVVVTAENAQQARELRSGN
                     APDLVLLDIWMPDTDGVTLLKEWQRDGLLTMPVIMMSGHATIDTAVEATRIGALDFLE
                     KPIALQKLLKAVQQGLSHGCEPVRPLSYIRTTVTAEHDSIAHHPSPMLSSYVLPSAVN
                     VLHHGHGMAPVPAYALPVDNGPSISFNLPLREARDAFERAYFEYHLVRENGSMTRVAE
                     HTGLERTHLYRKLKQLGVEPGKLSRKSV"
     gene            complement(1780..>3384)
                     /locus_tag="VFA14_07050"
     CDS             complement(1780..>3384)
                     /locus_tag="VFA14_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011979382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZS81166.1"
                     /translation="VVISVPTLPKAISLQDDSRFLQILQPVPSYLATNAETLRLAYSE
                     YQQRSVARSGLRKIYIVTLTLTLLLTIFGAIVSAFLIASDLAKPLLLLAEGTKAVAEG
                     NLSPRPIVSTSDELGTLTQSFNIMTRQLLEARTLVEKNRAELENAKAYLESVLANMSA
                     GVMVLDSEFRIVSINDSVCRILGYGLTGCIGTPLHTIEGQARFAETIIKAFSEQRAQQ
                     ASDAAQDSLHWQQQIELSRHGGTPDGSAGTVQSENSREGSSGSGKIILLARGSHLPVE
                     SGVGYVVVFDDISKVISAQRSIAWGEVARRLAHEIKNPLTPIQLSAERLQMRLSDKLL
                     PQDAAILEKSTTTIVNQVTAMKHMVDDFREYAKAPPAKPAALDLNALIEEILHLYLAG
                     DGRDGIHVKLAPDLPRVMGDATQLRQVIHNLLQNAQDAIAENDDMAPCIEVATELLNY
                     QDSAGLARSAVRLMITDNGSGFDPMILSNAFEPYFTSKPKGSGLGLAMVKKIIDEHGG
                     RIDIQNRSDTRGAKVLILLLKLTPDT"
BASE COUNT          736 a          931 c          919 g          798 t
ORIGIN      
        1 ttcgtcagcg catgacagca accatcggca gcatgatcga caagttgata cgcacgcagc
       61 ctgacgctac catcactgcc ggcactggcg gcattcgtcc ttatccgcag gaactggatg
      121 ccgctgccga caatttagcg ctgcatatgt tggtacgcgc cggttacagc atcgacaact
      181 atgcggcctt ctggcagcgt ctggcgacgc agtatccagc cagcgtgcgg aacgcttaca
      241 ccgcgctgca tccggcgacg gcttatcgca tgaccgtgat caacgaggca gtacgaacgc
      301 tcaaaactgg aaaaaccacc gaaaaccgca agcgtccggc cactccacct gttcaggggc
      361 aagtttagcg gtgctggtag aagccagaca gaaaatccat caatccggtt atcttcagtg
      421 cgacaggctg ctagtgcaat gccagcgaat agcgcatcat cagatcgtac ctgctgagga
      481 aggtatcgat atcctccatt gtcggcgccg ttgcaatcag atcctgcaca tccctgcgaa
      541 acagttcagc ggcggggcca tcgatgtaga tttcgcgccg gcccggttta ttcgtaattt
      601 cataaccgcc gaagcgcatg gcttcgtgat tccagtcgac gccgaattca aggacactgt
      661 attgctcact gttgtagatc agattcattg cagctcctta tacacatacc tgatgttgtg
      721 aaggagatga cgccatatcc gcagatttca aggtgtcccg atggatcgtt cccgtacttc
      781 cctgtgcgta tcgatggcag cgccgggcgc ctgcgtgtaa tcagacgctt tttcgcgaca
      841 gtttgcctgg ctcgacgccc agttgtttga gtttacggta taagtgcgtg cgctccagtc
      901 cagtgtgttc ggctacacgt gtcatgctac cattttcgcg caccaggtgg tattcaaagt
      961 aagcgcgctc gaaagcatcg cgcgcttcgc gcagtggcag gttgaaagaa atactggggc
     1021 cattgtcaac aggtagcgca tacgccggta caggggccat gccgtgtcca tgatgcaata
     1081 cattgacagc gctcggcaat acgtaggatg acaacattgg actgggatgg tgagcgatgc
     1141 tatcgtgttc tgccgttacc gtcgtccgga tataggatag cggacgcacc ggttcacagc
     1201 catgtgacag accttgctga actgccttga gcagtttttg cagggcgatc ggtttttcca
     1261 ggaaatcgag cgcgccgata cgggtcgctt caacggcggt gtcgatggtg gcatgaccgg
     1321 acatcataat gaccggcatg gtcagcaaac catcgcgctg ccactctttg agcagcgtga
     1381 cgccatcggt gtcaggcatc cagatatcga gcaataccag atcaggagca ttacctgagc
     1441 gcagttcgcg tgcctgctga gcgttttctg cagtgacgac gacatgccct tcatcgccca
     1501 gaatttccga gagcaattcg cgaatgccca tttcgtcatc gacgaccagg atgttagcca
     1561 tgtgtctgct tccctcatta aatattgttg agattctctg aaagcctgtc tgcgatgcaa
     1621 atcgcagaca ggtttcgagt caactctaaa cacttcacaa agcaccgctg tgtcatcgat
     1681 ctggctggcg ctcatcaaca ccggattaat ttggcgactt tattgaatcc agtaaagacc
     1741 ccctgtagca acggcaaccg aggataaaat ccttgatcat tatgtatcgg gagttaactt
     1801 taacagcaaa atcagtactt ttgcgcctct tgtgtcgctc cgattttgta tatcgatccg
     1861 tcccccgtgt tcgtcgatga tttttttgac cattgccaag cccaaacctg agccttttgg
     1921 ttttgatgtg aaatatggct cgaatgcatt cgatagaatc atcggatcaa agccagatcc
     1981 attatctgta atcatcaaac gcacggcgct acgtgccaat ccggccgaat cctgatagtt
     2041 gagcagttct gtagcgactt cgatacacgg cgccatatcg tcgttctcgg cgatggcatc
     2101 ctgtgcattt tgcaacagat tatggatgac ctggcgcaat tgcgtggcgt cgcccattac
     2161 tctcggtaaa tctggcgcca gtttgacatg gatgccatca cgaccgtcac cggccaggta
     2221 taaatgtaaa atttcttcga tcagcgcatt cagatccagc gcagcaggtt tggccggcgg
     2281 tgccttcgca tattctcgga agtcgtcaac catatgtttc atggcagtga cctgattcac
     2341 aatcgtggtt gtactttttt ccagtatcgc ggcatcctgc ggcagcagtt tgtcggacag
     2401 gcgcatctgc agacgctcgg cggagagctg gattggcgtc aacgggtttt tgatctcatg
     2461 cgccagacgt cgcgctactt cgccccaggc gatagaacgc tgggcggaaa tgaccttgct
     2521 gatgtcgtcg aataccacta catagccgac accgctttcc accggcaaat gcgatccgcg
     2581 tgccaacagg ataatcttgc cgctacctga acttccctcc ctgctgtttt ccgattgcac
     2641 ggtgccggcg ctgccgtccg gcgtgccgcc gtggcgcgac agttcgatct gctgctgcca
     2701 gtgcagacta tcctgcgcgg cgtcggatgc ctgttgcgcg cgttgctccg aaaaggcttt
     2761 gatgatcgtt tcggcaaagc gcgcctggcc ctcgatcgta tgcaggggcg tgccgatgca
     2821 gccggtgaga ccatagccga gaatgcgaca caccgagtcg ttaatgctga cgatgcggaa
     2881 ttcactgtca agcaccatga cgccggctga catgttggcc agcaccgatt cgagataagc
     2941 cttggcattt tccagctcgg cgcggttctt ttcgaccagg gtgcgtgcct ccagcaactg
     3001 gcgcgtcatg atattgaatg actgggtcag cgtgccgagt tcgtccgaag tgctgacgat
     3061 ggggcgcggc gacaggttac cttcggccac cgccttggtc ccctcggcca gcagcagcaa
     3121 gggtttggcc agatcgctgg cgatcaggaa agcgctgacg atagcgccga aaatggtcag
     3181 cagcagcgtc aacgtcagcg taacgatata gatcttgcgc aggcccgaac gcgctactga
     3241 gcgctgctgg tattcgctgt aggccagccg cagcgtttcg gcgttggtgg cgaggtagct
     3301 tggtaccggc tgcagaattt gcaggaaacg actgtcgtct tgcaggctga tcgctttagg
     3361 caaagtcggt actgaaataa ccac
//
LOCUS       DASRDH010000305         4716 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_888400,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000305 DASRDH010000000
VERSION     DASRDH010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 4716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4716
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_888400"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="VFA14_07055"
     CDS             complement(<1..233)
                     /locus_tag="VFA14_07055"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004524507.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HZS81167.1"
                     /translation="MQLNASEISELIKSRIQGLGDSAEIRNQGTVISVSDGICRIHGL
                     SDVMQGEMLEFPGNTFGLALNLERDSVGAVVLGG"
     gene            complement(281..814)
                     /locus_tag="VFA14_07060"
     CDS             complement(281..814)
                     /locus_tag="VFA14_07060"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HZS81168.1"
                     /translation="MAEPATIARPYAEALFRVAQAGGLSAWADLVSEMAQAATHPDVK
                     VLAQNPALSDQQISATFASILKTPLGVQAQNFIDVLVRNDRLALLPEIAAQFHTLKNA
                     QEGTDDAQITSAFDLSGAQLEDVVAALEKKFGRRLTPTVKVDPALIGGVRVVVGDEVF
                     DTSVRAKLQQLHAALTA"
     gene            complement(816..1286)
                     /locus_tag="VFA14_07065"
     CDS             complement(816..1286)
                     /locus_tag="VFA14_07065"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HZS81169.1"
                     /translation="MNLNATLFAQFVVFFILAGFTMKFVWPRLINALDERAKKIADGL
                     AAADRGKADLAAAEKCIQAELAAAREEGQKRIGDAERRAQLIIEEAKKTAGDEAARII
                     AGAKADAEQQVNKAREELRGEVAVLAVKGAKQILKREINAAAHADLLNQLKTGL"
     gene            complement(1362..1607)
                     /gene="atpE"
                     /locus_tag="VFA14_07070"
     CDS             complement(1362..1607)
                     /gene="atpE"
                     /locus_tag="VFA14_07070"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HZS81170.1"
                     /translation="MTDVSFVALACGLIIGLGAIGACIGIAIMGGKYLEASARQPELM
                     NTLQTKMFLLAGLIDAAFLIGVGIAMLFAFANPFIAK"
     gene            complement(1685..2500)
                     /gene="atpB"
                     /locus_tag="VFA14_07075"
     CDS             complement(1685..2500)
                     /gene="atpB"
                     /locus_tag="VFA14_07075"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011350522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HZS81171.1"
                     /translation="MTAAEHAPTPSEYIVHHLGHLSSQHQTSIVDFSIINMDTIFWSV
                     CCGVIACLCMYLAARKATSGVPGRFQAFVEMVVEMVEDQSKAIVHGDRSFIAPLALTV
                     FIWVALMNSLDFLPVDLVSGIMRLAGMEEIHHRIVPTADLNGTLGIALGVLALMLYYS
                     IKIKGVGGFLHELFAAPFGIWLAPFNLLLNIIEFAAKTVSLGMRLFGNMYAGELLFLL
                     IALLGSAATAFGFVGHVIAGSIWAIFHILIVLLQAFIFMMLTLVYLGQAHEAH"
     gene            complement(2497..2841)
                     /locus_tag="VFA14_07080"
     CDS             complement(2497..2841)
                     /locus_tag="VFA14_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006461330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HZS81172.1"
                     /translation="MLRIILLQLAVMVVAACIAGLLGSVSALLSVLLGGICCVVPNGL
                     FALRLHTSTRRPDGANTMTFFFGEFIKIAMTLALMGAVVRLYHDVNWFAFVLGFIAVL
                     KSYLILLFKHRP"
     gene            complement(2961..>4716)
                     /gene="ilvD"
                     /locus_tag="VFA14_07085"
     CDS             complement(2961..>4716)
                     /gene="ilvD"
                     /locus_tag="VFA14_07085"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003819105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HZS81173.1"
                     /translation="IAVVNSFTQFVPGHVHLKDLGQLVAREIEAAGGVAKEFNTIAID
                     DGIAMGHGGMLYSLPSRDLIADSVEYMVNAHCADAMVCISNCDKITPGMLMAAMRLNI
                     PTVFVSGGPMEAGKVIKVIDNDKKLIKLDLVDAMIKAGDSRVPDADVAAVERAACPTC
                     GSCSGMFTANSMNCLTEALGLALPGNGTIVATHADRKELFLRAGRLIVELAQRYYEQD
                     DDAVLPRSIATRAAFENAMALDVSMGGSTNTVLHLLAAAHEAQVEFTMTDIDRISRKV
                     PCLCRVAPMTDKFHIEDVHRAGGIIAILGELARAGLLDTSLPTVHSKTLDAAIEKYDI
                     RISSDPAIHTLFRAAPGGVPSQVAFSQTERYGQNDLDRNAGCIRDREHAYSQDGGLAV
                     LYGNIAEKGCIVKTAGVDESILQFSGTARVFESQDAAVEGILGDKVQAGSVVIIRYEG
                     PKGGPGMQEMLYPTSYIKSKGLGKTCALFTDGRFSGGSSGLVIGHASPEAAEGGAIGL
                     VEEGDAIDIDIPSRTINLRVPAGELAHRRAAMEARGHAAWQPVDRGRVVSQALQAYAA
                     LTTSADRGAVRDLSQLKR"
BASE COUNT         1031 a         1392 c         1334 g          959 t
ORIGIN      
        1 ccgcccagaa cgacggcacc cacggagtcg cgttcgagat tcagcgccag accgaaagtg
       61 ttgccaggga actccagcat ttcaccttgc atcacgtcgg aaagaccatg gatacggcag
      121 ataccgtcgg acacggaaat aaccgtgcct tgattgcgaa tctcagcgct gtcgccaagg
      181 ccttgaatcc ggctcttgat cagttcgctg atttcagatg cgttgagttg catactcact
      241 cctaaaaatt ttttctatcg ctcaggctgt tacgccattc ttacgcagtc aaagcagcat
      301 gcaactgttg cagcttcgcg cgcaccgagg tatcgaatac ttcatcaccg acgaccacac
      361 gcacgccgcc gatcagggca ggatctacct tgacggttgg cgtcagcctg cggccgaatt
      421 tcttttccag cgcggcgact acatcctcta actgcgcgcc agacaaatcg aacgcgctgg
      481 tgatttgtgc atcgtcagta ccttcttgcg cgtttttcag ggtatggaat tgcgccgcga
      541 tttccggcag caaggccaga cggtcgttcc gcaccagaac gtcgatgaaa ttttgtgctt
      601 ggacgcccag cggagttttc agaatggaag cgaaggtggc agagatctgc tggtccgaca
      661 acgctggatt ttgcgccaaa accttgacgt cgggatgcgt cgcggcctgt gccatttcgg
      721 aaacgagatc ggcccaggct gacaagccgc cggcttgggc cacacggaac agggcttctg
      781 cgtaagggcg ggcgatcgtt gcgggttcgg ccatattaca gccctgtttt cagttggttc
      841 agcaagtcgg catgcgccgc tgcgttgatt tcacgcttca gtatctgttt ggcgcctttg
      901 actgcgagaa ccgcgacttc gccgcgtaat tcttcacgcg ctttgttcac ttgctgctct
      961 gcgtccgcct tggcgccggc gatgatgcgg gccgcttcgt cgccggcggt ttttttcgct
     1021 tcttcgatga tcagttgggc acgcctttca gcgtcgccga tacgtttctg accctcttcg
     1081 cgcgccgccg ccaactctgc ttggatgcat ttctcggcgg cagccaggtc agccttgcca
     1141 cggtcagcag cagccaaacc gtccgcaatc ttcttcgcgc gctcgtcgag cgcattgatc
     1201 aaccgtggcc aaacgaattt catcgtaaag ccggcgagga tgaagaagac cacgaactgc
     1261 gcaaacaatg ttgcattgag gttcacggta atttccttct cagaatatcg tgtgccggaa
     1321 tagcggtccg gcaaaagtga gaaccggcca tgccgactgg attacttcgc gatgaacgga
     1381 ttggcaaatg cgaacagcat ggcgataccg acgccgatca gaaacgcggc atcgatcaga
     1441 ccggccagca ggaacatctt ggtttgcagc gtgttcatta gttctggctg acgtgcggat
     1501 gcttcgagat acttgccgcc catgatcgcg ataccgatac aggcgccgat agcacccagg
     1561 ccgatgatca aaccacaagc caaagcaacg aaagagacat cagtcatgac aattccttag
     1621 aaagttaaag ttaatgcaaa aatcaaaaga cgaaccgaaa agctaaaacc gtacagcagc
     1681 cctgttaatg cgcctcatgc gcctgaccca ggtaaaccag tgtcaacatc atgaaaatga
     1741 atgcttgcag caacacgatc aggatgtgga aaatcgccca gatcgaaccg gcgatgacgt
     1801 ggccgacgaa gccgaacgcc gtggcggctg aacccagcaa ggcgatcagt aggaacaaca
     1861 gctcgcccgc atacatgttg ccgaacagac gcatgcccag cgacacggtc ttggcggcga
     1921 attcgatgat attcaacagc agattgaacg gtgccagcca gataccgaag ggtgctgcga
     1981 acagttcatg caggaagccg cccacgccct tgatcttgat gctgtagtac agcatcagcg
     2041 ccagcacgcc cagcgcaatg cccagtgtgc cgttcaggtc ggcggtagga acgatgcgat
     2101 gatggatctc ttccatgccg gccagacgca tgatgcccga caccagatcg accggcagaa
     2161 aatccagcga attcattagg gcgacccaga tgaacacggt caaggccagc ggcgcgatga
     2221 agctgcggtc gccatgcacg atggccttgg actggtcttc gaccatttcg acgaccattt
     2281 cgacgaaagc ctgaaaacgg cccggtacgc cggaagtcgc cttgcgcgcc gcgaggtaca
     2341 tgcacaggca ggcaatgacg ccgcaacata ccgaccagaa aatagtgtcc atgttgatga
     2401 tggaaaaatc aacgatgctg gtttgatgct gcgaagacaa atgtcccaga tggtgaacga
     2461 tgtattctga tggagtaggt gcgtgttcgg cagcagtcat ggtcggtgtt taaacagtaa
     2521 aatcaagtaa cttttgagta ccgcgatgaa acccagcaca aaggcgaacc aatttacatc
     2581 gtgatacagc cggacaactg cgcccatgag cgcaagagtc atggcaatct tgatgaattc
     2641 cccgaaaaaa aatgtcatcg tgttcgcccc atcaggcctg cgggtgcttg tatggagacg
     2701 cagggcgaac agaccgttgg gaacgacgca acagatcccc cccagcaata ccgacaacaa
     2761 cgcagatacg ctgcccagca agccagcgat acatgcggcg acgaccatga ccgcaagctg
     2821 caacaggatg atacgcagca tagatgtcca atatcatcgc gcggaccgca ctcattatgt
     2881 gagcaggcgg cagttcagaa acccggcgat tataattgcc gggtttcctg ttcgtcaaac
     2941 ttttctgagc ctgttcagga ttagcgcttg agttgcgaca ggtcgcgcac cgcgccgcgg
     3001 tcggcggagg tagtcagcgc ggcataggct tgcagcgcct gcgagacgac gcgcccgcga
     3061 tcgaccggtt gccaggcggc atgaccgcgc gcttccattg cggcgcggcg atgcgcgagt
     3121 tctccagccg ggacgcgtag attgatcgtg cgggacggga tgtcgatgtc gatcgcgtcg
     3181 ccttcttcca ccaggccgat cgcgccgcct tccgccgctt ccggcgaagc gtggccgatc
     3241 acgaggccgg aggaaccacc ggagaaacgg ccgtcggtga acagcgcgca tgtcttgccg
     3301 agacccttcg atttgatata cgacgttgga tatagcatct cctgcatgcc tggtccgccc
     3361 ttcggacctt catagcggat gatgacgacg ctgcccgcct gcaccttgtc gccgagaatg
     3421 ccttccaccg ccgcatcctg actctcgaac acgcgcgccg tgccggagaa ctgcaagatg
     3481 ctctcatcca cgccggcggt cttcacgatg cagcccttct ctgcgatatt cccgtataga
     3541 accgccagac cgccgtcctg cgaatacgcg tgttcccggt cacgaatgca gccggcgttg
     3601 cggtcgagat cgttctgccc gtagcgttcc gtttgcgaaa acgccacttg cgacggcacg
     3661 ccgcccggcg ctgcgcggaa cagggtgtgg atggcaggat cgctgctgat gcggatgtcg
     3721 tacttctcga ttgctgcgtc cagtgtcttg ctatgcaccg tcggcaatga agtgtcgagc
     3781 aggccggcac gcgccaattc gccaaggatg gcgatgatgc cgccggcgcg atgcacatcc
     3841 tcgatgtgaa acttgtccgt catcggcgcc accctgcaca ggcagggtac tttgcgtgaa
     3901 atacggtcga tgtcggtcat ggtgaactcc acctgcgctt cgtgtgctgc ggccagcaga
     3961 tgcagcacgg tgttggtgga gccgcccatc gagacgtcca gcgccatggc gttttcgaat
     4021 gccgccctgg tggcgatgct gcgcgggaga acggcgtcat cgtcctgttc gtaatagcgt
     4081 tgcgccagtt cgacgatcag ccggcccgcg cgcaggaaca gctctttacg gtcggcatgc
     4141 gtggcgacga tcgtgccgtt ccccggcaac gccagaccca gggcctcagt cagacaattc
     4201 atcgaatttg ccgtaaacat gccggaacac gaaccgcagg tggggcaggc ggcgcgttct
     4261 actgcggcta catcggcatc tggcaccctg ctgtcgccag ccttgatcat tgcgtcgacc
     4321 agatccagct tgatcagctt tttgtcgttg tcgatgacct tgatcacctt gcctgcttcc
     4381 atcggcccgc cggacacgaa cacggtgggg atgttcagcc gcatcgccgc catcagcatg
     4441 cccggcgtga tcttgtcgca gttcgagatg cacaccatcg cgtcggcgca gtgcgcgttg
     4501 accatatatt cgaccgagtc ggcaatcaaa tcacgtgacg gcagcgaata cagcatgccg
     4561 ccgtgcccca tcgcgatgcc gtcgtcgatg gcgatcgtat tgaattcctt cgctacaccg
     4621 ccggccgctt cgatctcgcg cgctaccagt tggcccagat ctttcagatg cacatggccc
     4681 ggtacgaact gcgtgaatga attgaccacg gcaatg
//
LOCUS       DASRDH010000306         2099 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1673186,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000306 DASRDH010000000
VERSION     DASRDH010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1673186"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..856)
                     /locus_tag="VFA14_07090"
     CDS             complement(<1..856)
                     /locus_tag="VFA14_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HZS81174.1"
                     /translation="MATDYHHGVRVIEVNEGARPIRTMSTAVIGLIATADDADPAQFP
                     LNTPVMLTNVNNVLGKAGTTGTLKRTLTAIGLQTKPLTIVVRVAEGEDEMETTSNVIG
                     GVDGNGKYLGIKALLAAQAQLGVKPRILGAPGLDTQAVANALASAAQSLRAFGYVSAW
                     NCKTKEEAIAYRDRFGQRELMVIWPNFLDWNSDAQAQAPMPAAAYALGLRAKLDEEIG
                     WHKTLSNMVVNGPLGISADVFWDLQDPATDAGLLNSKEVTTLINRSGYRFWGSRTCEA
                     QGGYFPFEN"
     gene            complement(918..1676)
                     /locus_tag="VFA14_07095"
     CDS             complement(918..1676)
                     /locus_tag="VFA14_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HZS81175.1"
                     /translation="MNKTVYNYHPQTGEFINEVRADESPLEPGVILIPAFATTAKPPK
                     TVTREVAVFADGKWRIKADWRYISLFSTADGSAVTIGEIGKTPADVGATDQPMPSSAH
                     AWTNGSWQLDPVKQAAQFDRAKLQALATIDQFHAETVQKLVGNPTQVERDTWELKLEV
                     ANAITGKAAVGATGQAFLVNAGIATDTARSAWAASVLARWSAYAQVIGLAEKLRNTAR
                     TAVKAATDEASLKAALDAQRAAADAVVAHLLKDA"
     gene            complement(1690..>2099)
                     /locus_tag="VFA14_07100"
     CDS             complement(1690..>2099)
                     /locus_tag="VFA14_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015985103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HZS81176.1"
                     /translation="TGYLKANGVAVSRATYAALFAAIGTTFGAGDGSTTFTLPDLRGE
                     FLRAFDDGRGVDSGRTFGSSQTDLFKEHNHRFINEYNTPNMRIVAYSDDNSEGVDSVN
                     TTGLRCWTYISMENTGGSETRPRNIALLACIKF"
BASE COUNT          370 a          617 c          644 g          468 t
ORIGIN      
        1 agttctcgaa agggaaatag ccgccttgcg cctcacaggt acgcgaaccc cagaagcgat
       61 agccgctacg gttgatcagt gtggtaactt ccttgctgtt cagcaggccg gcatcagttg
      121 ccggatcctg cagatcccag aagacgtcgg cgctgatgcc cagcgggccg ttgacgacca
      181 tattggacag tgtcttgtgc caaccgattt cctcgtcgag tttcgcgcgc aagccaagcg
      241 cataggctgc ggccggcatc ggcgcctgtg cttgcgcatc gctgttccag tccagaaaat
      301 tcggccagat caccatcaat tcgcgctggc cgaaccggtc acggtaggcg atcgcttctt
      361 ccttcgtttt gcagttccat gcactgacat aaccgaatgc gcgcaggctc tgcgcggcgc
      421 tggcaagcgc attggcaacc gcttgtgtat cgagaccggg cgcgccaagg atgcgcggct
      481 tcacacccag ttgcgcctgt gccgcgagca gcgccttgat gccaagatac ttgccattgc
      541 catcgacgcc gccaatgacg ttgctggtgg tttccatttc gtcttcacct tctgccacgc
      601 gcaccacgat cgtcagcggc ttagtctgta gaccgatggc ggtgagcgtg cgtttgagcg
      661 tgccggtcgt gccggctttg ccgagaacat tgttcacgtt tgtcagcatg acaggcgtat
      721 tcagcgggaa ctgggcagga tctgcatcgt ccgcagtggc gatcagtccg atgacggccg
      781 tggacatggt gcgaatgggg cgcgcacctt cgttgacttc gatgacacgg acgccgtggt
      841 ggtagtcggt tgccattgca tgactccttt gttgatgggg ttggtcgggt tactcgatca
      901 ggggctgtga cgcgacttca ggcgtctttc aacaggtggg ccaccaccgc atcggcagcg
      961 gcgcgctgcg catcaagcgc ggccttgagt gatgcctcat ccgtggctgc cttcaccgct
     1021 gtgcgggctg tgttgcgcag cttctcggcc agaccgatca cttgggcata cgccgaccac
     1081 ctggccagca ccgaagccgc ccaggccgac ctggccgtat ccgtggcgat gccggcattg
     1141 accagaaacg cctggccggt agcaccgacc gccgccttgc cggtgatcgc gttggctact
     1201 tccagcttca gttcccaggt gtctctttcg acttgagtcg ggttgccgac cagcttctgg
     1261 acggtttcgg cgtggaactg gtcaatcgtg gccagcgcct ggagcttggc gcggtcgaac
     1321 tgtgccgcct gcttgaccgg atcgagctgc caggagccgt ttgtccaggc atgcgcggag
     1381 cttggcatgg gctggtccgt cgcgccaacg tcagccgggg tcttcccgat ctcgccgatg
     1441 gttaccgcgc tgccatccgc tgtactgaac agggatatat agcgccagtc ggccttgata
     1501 cgccatttgc catcagcgaa gacggcaact tctcgggtga ccgtcttcgg aggcttggca
     1561 gtcgtggcga atgccgggat gagaatcacg ccgggttcga gcggcgattc gtcggcacgg
     1621 acctcattga tgaactcgcc ggtttgcggg tggtagttgt agacggtctt gttcattgtg
     1681 tgtccttgat cagaatttga tgcaggcgag cagcgcgata ttgcgggggc gggtttcact
     1741 gccaccagtg ttctccatac tgatgtatgt ccagcagcgc agccctgtcg tattgacgct
     1801 atccaccccc tcactgttgt catccgaata cgcaacgatc cgcatgtttg gcgtgttgta
     1861 ttcgttgatg aatcggtggt tgtgctcctt gaagagatca gtttgagagg agccgaaagt
     1921 tcggccacta tcaacaccac gtccatcgtc aaatgcacgc aggaactctc cccgcaaatc
     1981 aggaagcgtg aaggtggtcg atccatcgcc agcaccgaaa gtggtgccaa tggctgcgaa
     2041 cagcgccgcg taggtggcac gtgaaaccgc cacgccgttg gccttcagat aaccggttg
//
LOCUS       DASRDH010000307         3091 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_639566,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000307 DASRDH010000000
VERSION     DASRDH010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 3091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3091
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_639566"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /locus_tag="VFA14_07105"
     CDS             <1..627
                     /locus_tag="VFA14_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HrcA family transcriptional regulator"
                     /protein_id="HZS81177.1"
                     /translation="ESVFQQIEFLRLSEKRILLVIVGPGGDVQNRLLQTDIDYTPSQL
                     TQAANYINQHYGGLGFDEVRLRLQNELRQLRDDMTSLMQAAVEAGSDAMTDDVVISGE
                     RNLLSIADLSSNMTSLRKLFDMFEQKTSLMQLLDISSKASGVQIFIGGESELMPMDDM
                     SMVTAPYAVNGKIVGTLGVIGPTRMAYERVIPIVDITARLLSGVLSNN"
     gene            complement(695..1900)
                     /gene="hemW"
                     /locus_tag="VFA14_07110"
     CDS             complement(695..1900)
                     /gene="hemW"
                     /locus_tag="VFA14_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005665935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HZS81178.1"
                     /translation="MIPIELVSSVRSSTFEHGSLQLTALPPLSLYVHFPWCVKKCPYC
                     DFNSHEIKGDFPEDAYLDALRADLESALPLIWGRRIHSIFIGGGTPSLLSAAGLDRLL
                     SDIRALLPMDADIEITMEANPGAFEAEKFASCRASGINRLSLGIQSFNSTHLAALGRI
                     HDAQQARRAVDIAHANFDNFNLDLMYALPSQSVEEARRDIATAIACAPPHLSLYHLTL
                     EPNTLFAKYPPLVPGEDASADMQDMIVELTAAAGYQHYEVSAYARPKHRARHNLNYWQ
                     FGDYLGIGAGAHSKLSFPRRIVRQMRHKHPQTYLDRMRAGHALQEKAEIGRDALGFEF
                     MLNALRLQDGFPVHLFTERTGLSINDIEAALDEAEKKGLLFRDHLTIRPTGLGRRFLN
                     DLQAMFLVA"
     gene            complement(1900..2484)
                     /gene="rdgB"
                     /locus_tag="VFA14_07115"
     CDS             complement(1900..2484)
                     /gene="rdgB"
                     /locus_tag="VFA14_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020701662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HZS81179.1"
                     /translation="MLMTIVLASSNQGKCEEFAALLSEIGLDVCLQSAFNIREADEPF
                     ATFVENALTKARHAARLTGLPALADDSGICANALGGAPGVRSARYAGEPRSDAANSAR
                     LIADLAMYADKSAYYYCVLVYVRHAQDPQPVIADGAWHGEIIADARGEGGFGYDPFFL
                     LPTLGKTAAELSATEKNAYSHRGQALRALLEKLR"
     gene            complement(2501..>3091)
                     /gene="rph"
                     /locus_tag="VFA14_07120"
     CDS             complement(2501..>3091)
                     /gene="rph"
                     /locus_tag="VFA14_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008112378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HZS81180.1"
                     /translation="SIEEKVPGFLKGKGQGWMTAEYGMLPRSTHIRMDREAARGKQSG
                     RTQEIQRLIGRALRAAFDLEAFGERTLHLDCDVLQADGGTRTAAITGAMVAAYDAFSA
                     LQGRGMISAIPLKYFVAAISVGIYRGVPVLDLDYLEDSSCDTDMNIVMTDAGHFVEVQ
                     GTAEGAAFDRATLDSLLDIASKGIRELIALQKAALD"
BASE COUNT          668 a          905 c          900 g          618 t
ORIGIN      
        1 gaatcagtct tccagcagat cgagtttctg cgcctgtccg aaaaacggat tctgctggtc
       61 atcgtcggcc ccggcggtga cgtgcagaac cgcctgctgc aaaccgatat cgactacacg
      121 ccatcgcaac tgacacaggc cgccaattac atcaaccagc attacggcgg actcggcttc
      181 gacgaggtgc gtctgcgcct gcaaaacgag ctacgccagt tacgcgatga catgaccagc
      241 ctgatgcagg ctgcggtcga agccggcagc gacgccatga ccgacgatgt cgtaatctcg
      301 ggtgagcgca atctgctcag catcgccgac ctgtcgtcga acatgacttc actgcgcaag
      361 ctgttcgaca tgttcgaaca aaaaaccagt ctgatgcaat tactggacat ctccagcaaa
      421 gccagcggcg tacagatttt catcggcggc gaatcggaac tgatgccgat ggacgacatg
      481 agcatggtca ccgcgccgta tgcagtcaat ggcaaaatcg tcggcacgct gggggtgatc
      541 ggcccgacgc gcatggccta cgaacgtgtc atcccgatcg tcgacatcac cgctagactg
      601 ctgtcaggcg tactcagtaa taactgaagc tgcgacaaat ctcgtcaaat cccggtaaag
      661 tttagcgact ccggcgattg atattttcaa tagatcatgc aaccagaaac attgcctgca
      721 gatcgtttaa aaaacggcgc cccaatccgg tcggacgaat cgtcagatgg tcgcgaaata
      781 gcagaccctt tttctcggcc tcatcgagcg cagcttcaat atcgttgatg ctcaggccgg
      841 tgcgttcggt aaatagatgg actggaaaac cgtcctgcaa acgcagggca ttgagcatga
      901 actcgaaccc gagcgcatca cggccgattt cagccttttc ctgaagggca tggccggctc
      961 gcattctgtc caggtaagtc tgcggatgtt tgtggcgcat ctgccgcacg atgcggcgcg
     1021 gaaacgataa tttggaatgt gccccggcgc caatgccgag gtaatcgccg aactgccagt
     1081 aattcagatt atggcgcgcc cgatgcttcg gcctagcata ggccgacact tcataatgct
     1141 ggtaaccggc tgcagccgtc agttcaacga tcatgtcctg catgtcggcg ctggcgtctt
     1201 cacccggcac cagcggcggg tatttggcga acagcgtgtt cggttccagc gtcaagtgat
     1261 acagcgagag atgcggcggc gcgcaggcaa tcgcggtggc aatgtcacgc cgcgcttcct
     1321 ccacgctctg cgatggcagg gcatacatca gatcaaggtt gaaattgtca aaattggcat
     1381 gtgcgatgtc gactgcccgg cgcgcctgtt gggcatcatg gatacggccg agcgccgcca
     1441 ggtgcgtgga attgaagctt tgtatgccta gcgacaggcg gttgataccg ctggcgcggc
     1501 aggatgcgaa tttctctgcc tcaaaggcgc ccggattggc ctccatggtg atttcaatat
     1561 cggcatccat aggcagcagg gcgcgaatat cggacagcag gcgatcgagt cccgccgccg
     1621 atagcaaact cggcgtgccg ccaccgatga agatggaatg gatcctgcga ccccagatca
     1681 gcggcagtgc cgactccagg tcagcgcgca aggcatcgag ataggcgtct tcgggaaagt
     1741 cacccttgat ttcatgcgaa ttgaagtcac aatacggaca tttttttacg caccaaggga
     1801 aatgcacgta tagcgacaac ggtggcaggg ccgtcagttg caggctgcca tgctcgaacg
     1861 tggaagaccg gacactgctg acaagctcaa tcgggatcat catcgcagct tttccagcaa
     1921 tgcgcgcaga gcctggccac gatgcgagta ggcgtttttt tccgtggcgc tcagttcggc
     1981 ggcggttttg cccaaggtcg gcaacaggaa aaaaggatcg tagccaaaac cgccctcacc
     2041 gcgcgcgtca gcgatgattt caccatgcca ggcgccatcg gcgatcaccg gctgcgggtc
     2101 ttgcgcatgc cgcacataca ccagcacgca atagtaataa gcggatttat cggcgtacat
     2161 agccagatcg gcgatcagcc tggcgctgtt ggcggcatct gatcgcggtt cgccggcata
     2221 gcgcgctgac cggacgccgg gcgcgccgcc gagcgcgttg gcgcagatgc cggaatcgtc
     2281 ggccagcgca ggcagaccgg tcaggcgcgc cgcgtgacgc gccttggtca gcgcattttc
     2341 gacaaaggtg gcgaacggtt catctgcctc ccggatgttg aacgcgcttt gcaaacacac
     2401 atctaggccg atttcgctga gcaaggcggc gaactcctcg cattttccct gattactgga
     2461 ggccaggacg atggtcatga gcaggtgctt ttcctattca tcaatccagc gcggcctttt
     2521 gcagggcgat caactccctg atgcccttgc tggcgatatc cagcaggctg tcgagcgttg
     2581 cgcgatcgaa cgctgcgccc tcggctgtcc cctgcacttc aacgaagtgt ccggcatcgg
     2641 tcatgacgat gttcatgtcg gtatcgcagc tggaatcttc cagataatcc agatccagca
     2701 ccggcacacc acggtagatt ccgaccgaga tggcggcaac gaagtatttg agcggaatgg
     2761 cgctgatcat gccacgccct tgcaacgccg agaacgcatc gtaagcggcg accatggcgc
     2821 cggtgatggc ggcggtacgt gtaccgccgt cggcttgcag gacgtcgcaa tcgagatgca
     2881 gcgtgcgctc accgaaagcc tccagatcga aggcggcgcg cagggcgcgc ccgatcaggc
     2941 gctgaatttc ctgagtgcgg ccggattgct tgcccctggc ggcttcacga tccatgcgaa
     3001 tgtgcgtcga gcgtggcagc atgccatatt ctgcggtcat ccagccctgc cccttgccct
     3061 tgaggaaacc cggcactttt tcttcaatgc t
//
LOCUS       DASRDH010000308         2003 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_642818,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000308 DASRDH010000000
VERSION     DASRDH010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_642818"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            21..1181
                     /locus_tag="VFA14_07125"
     CDS             21..1181
                     /locus_tag="VFA14_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012336575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent formate dehydrogenase"
                     /protein_id="HZS81181.1"
                     /translation="MAKVLCVLYPDPETGYPPKYARDSIPAITSYANGQTAPTPKGPL
                     GFKPGQLVGCVSGELGLRPYLKKLGHDLIVISDKDGPNSDFEKHLPDAEVVISQPFWP
                     AYLTKERIAKAKKLKLALTAGIGSDHVDLEAAARAHITVAEVTGSNSISVAEHVVMMV
                     LSLVRNYLPSHEIAVKGGWNIADCAQRSYDVEGMHFGAIAAGRIGLAVLRRLKPFGLK
                     LHYTDVHRLSPDIEKELGLTFHPDAESLVKVVDIVNLQMPLYPSTENFFDDAMIAKMK
                     RGSYIINTARGKLVERDAIARALKSGHIAGYAGDVWFPQPAPADHPWRTMPWNGMTPH
                     ISGTSLSAQARYAAGTLEILESYFSGTPIREEYLIVNSGALAGTGAQSYKLT"
     gene            1603..>2003
                     /locus_tag="VFA14_07130"
     CDS             1603..>2003
                     /locus_tag="VFA14_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006465546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HZS81182.1"
                     /translation="MDTTLALKALIMGIVEGLTEFLPISSTGHLILAGSLLDFTGPAF
                     TREKAAVFEIVIQAGAILAVCWEYRIRIAGVLGGLFSDRQAQRLVINLIIAFLPAAIL
                     GFLFSQKIKAALFTPAPVALAFIAGGVIILWV"
BASE COUNT          438 a          615 c          536 g          414 t
ORIGIN      
        1 actgaaaaag gagctatccc atggccaagg ttctctgcgt actgtaccct gatcctgaaa
       61 ctggctaccc tccgaaatat gcccgcgaca gcattcccgc catcaccagc tatgccaatg
      121 gtcagaccgc gccgaccccc aaagggccgc tgggtttcaa gcctggccag ttggtcggct
      181 gcgtttccgg cgaactgggc ctgcgcccat atctgaaaaa actcggtcat gacctgatcg
      241 tcatcagcga caaagacggc ccgaactccg acttcgagaa gcatctgccg gatgcggaag
      301 tggtgatttc ccagccgttc tggccggcct atctgaccaa ggagcgcatc gccaaggcca
      361 aaaaactgaa gctggcgctg accgccggta tcggttccga tcacgtcgat ctggaagcgg
      421 cagcgcgcgc gcatatcaca gtggccgaag tcaccggctc gaactccatc agcgtggcag
      481 aacatgtcgt gatgatggtt ttatcgctgg tgcgcaatta cctgccgtca cacgaaattg
      541 cggtcaaagg cggctggaat atcgctgatt gcgcgcaacg cagctacgat gtcgaaggca
      601 tgcacttcgg cgccattgca gcaggccgca tcggcctagc tgtactgcgc aggctcaaac
      661 ctttcggcct gaaactacac tataccgacg tgcatcgcct cagtcccgac atcgagaaag
      721 agctgggcct cacttttcac cccgatgcgg aatcactggt gaaagttgtc gatatcgtca
      781 acctgcaaat gccgctctat ccctcgacgg agaatttctt tgatgatgcg atgatcgcca
      841 aaatgaagcg cggttcttac atcatcaaca cggcgcgtgg caaactggtc gagcgtgacg
      901 ctatcgcgcg cgccctgaaa tccggccata tcgcgggata tgcgggcgat gtatggttcc
      961 cgcagcccgc accggctgac catccgtggc gtaccatgcc ctggaacggc atgacgccgc
     1021 acatctccgg cacctcgcta tccgcgcagg cgcgttatgc ggcaggcacg ctggaaattc
     1081 tggaaagcta tttcagcggc acaccgatcc gcgaagaata tctgatcgtc aatagcggcg
     1141 cattggccgg cacaggcgct caatcctaca aactgacgta aaaccgtgtt gatcttgccg
     1201 ccgctcacag cggcggcctg tacgcctcca tccaagccag actctaatca tcaccgtact
     1261 aatctcgggg ggcagatcaa aggcaacaaa cgagaacggt atgaaagact cagctcgctg
     1321 acaccgctgt actggcagcc gaaaaggaaa cgcctgacga taattatccc ttctgtggat
     1381 cgcttgcccg cacaggatcg atccggatat ctgcctgcgt ggttagaaag cgcttgaaat
     1441 tctgatggcg ctggacacga ggctgtatcg cgatgccgga catgagattt tcctgcgcca
     1501 aaagcaaaaa gcggcatctt gctcgccaag tcagtaaaat gctggcttca tcccgcgtga
     1561 cagccgtaat cggcgggttc ctgactctcg tcttccttca gcatggacac aactctcgcg
     1621 cttaaagcgc tcatcatggg catcgtagaa ggcctgaccg aatttctgcc gatttcctcc
     1681 accggccatc tgattctcgc cggcagcctg ctcgatttca ccggaccggc ttttaccagg
     1741 gaaaaagccg ctgtgtttga aatcgtcatt caggccggcg ccatcctggc agtgtgctgg
     1801 gaataccgta tacgcatcgc cggcgttctg ggcggactat tcagcgaccg ccaggcgcag
     1861 cgcctggtca tcaacctgat catcgccttc ctgcctgccg ccatcctcgg cttcctgttc
     1921 agccaaaaaa tcaaggccgc gctgtttacg ccggcgccgg tggcgcttgc cttcatcgcc
     1981 ggcggggtga tcattctttg ggt
//
LOCUS       DASRDH010000309         4897 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1140265,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000309 DASRDH010000000
VERSION     DASRDH010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 4897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4897
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1140265"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..463)
                     /locus_tag="VFA14_07135"
     CDS             complement(29..463)
                     /locus_tag="VFA14_07135"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZS81183.1"
                     /translation="MSDFSERLKSERKKLGLNQADFGAAGGVKKDTQLNYERGDRNPD
                     YAYLQGIAAIGADVSYLLFGVIDQSRLSNDENEVLVGYRALDVRGKAGVLALIGSMGE
                     KGNAPRAVFHGNVAQVVQGNQTTKDVTFTVESGNKKKNLEHD"
     gene            542..745
                     /locus_tag="VFA14_07140"
     CDS             542..745
                     /locus_tag="VFA14_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS81184.1"
                     /translation="MSKSPNSRFRSSSLPDSQVVIFRMKKEEIAIADRLARLDRRSRS
                     SFIRNVLLKGIAEIERHQQHKAA"
     gene            755..964
                     /locus_tag="VFA14_07145"
     CDS             755..964
                     /locus_tag="VFA14_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006401795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS81185.1"
                     /translation="MYADPKRLRDNRLTLRFDDYEHQLIIALANYQGEQPSTLLRQIV
                     MREATALLDEASVSRGDSCAKAAIR"
     gene            1168..1452
                     /locus_tag="VFA14_07150"
     CDS             1168..1452
                     /locus_tag="VFA14_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013696211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HZS81186.1"
                     /translation="MNGGVSMKCPYCQSRAIARTSKAMSLTLREIIYSCRNHECGASW
                     AASLEATRLLSPSSMPNPHVRLPLSPHIRAAELISQIASSPQLKRDTQPP"
     gene            1553..1981
                     /locus_tag="VFA14_07155"
     CDS             1553..1981
                     /locus_tag="VFA14_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZS81187.1"
                     /translation="MGNNIEITVRSAAKTAHKDRAVQDHPRSARLPAAPEVQFSGRQT
                     GREMIVAAPVVSREQMQRVHEALRITTPLDACTPMMLRTLACIAHCWRDRIPANLWRD
                     LDSYLDPTPNESYRQHQLRCPVERDPELIDIKRRAAGDND"
     gene            1987..4692
                     /locus_tag="VFA14_07160"
     CDS             1987..4692
                     /locus_tag="VFA14_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HZS81188.1"
                     /translation="MNQDLQRDVLERLSEFKFRKEQRGYLRGGTCPSCHKKELYTHAD
                     HPWVVRCGRLNHCGWEGHVKELYPDLFEKWSERYPSTPENRNPNAAADAYLKFGRGFD
                     LAKIQGAYTQEQYFDRDLRIGSVTVRFSMANGYWERLIDQPQRFGKKKARFSFGLDYQ
                     GLWWQSATLQLLSVKEIWIVEGIFDAIALMHHGIAAVSAMSSSNYPEKALADLAASRG
                     NKDVTLVWALDGDAAGRSFTLKHVQRATEAGWQCKAAQIPQTGKNKIDWNDAHQRDRL
                     QQSHIDAYLYYGSLLIAKTAAEKALLIYNKENRHEFDFEFRRRLYWFSLNIVEYQKVR
                     DAMDKDGTEDDALPEQTKREKALAASMSLRSIANCHPQPLYYQANMITDESWYYFRVD
                     FPHDGAAIKNTFTASQLSSAAEFKKRLLGIAPGALFTGTSQMLDRIIQDKLENIKRVE
                     TIDYIGYSIDHGCYVLGDVAVKGGALHDLNSEDYFDIGRLSIKSLNKSVSLRINRDRS
                     EYTTEWLDLLWCAFGAKGLAALTFWFGSLFAEQIRKAHSSYPFLEIVGDAGAGKTTLI
                     ELLWKLLGRSGYEGFDPSKSTPAARARNFSQVSNMPVVLIESDRERVDGEKSHIKSFD
                     WDELKTAYNGRSIRARGMATGGNETYEPPFRGAVVIAQNTPVAASEAILSRIVHLYFD
                     RSTQTPASGAAADAIKYLTAEQVSGFVIEAARREKDILQLVIERTPIHQKELRTLPNI
                     KMPRIVETHAQMLALADALAMLVKLPADRHQALCAQIIDMTSERQTVINGDHPIVQEF
                     WEAFDFLNGNPNSCLDHSADPAVIAVNLNHFVQVASEKRQQIPLLRDLKKVLKTSSRR
                     KYIEQKAVNSTIYRNELPVMNGNKPTSTTVKCWVFSNPDYPKGDR"
BASE COUNT         1124 a         1480 c         1338 g          955 t
ORIGIN      
        1 gaaggacaat atcataggga tatcgccctc aatcgtgttc aaggttcttt tttttattac
       61 cgctttcgac ggtgaaggtc acgtccttcg tcgtctgatt cccctgcacg acctgagcga
      121 cattgccgtg gaaaacagcg cgcggcgcat ttcccttttc tcccatgctg ccgatcagcg
      181 ccagcacgcc ggccttgccg cgaacatcga gcgcacgata accaaccagc acttcgttct
      241 cgtcattact caatcgcgac tgatcaataa caccgaaaag aagatacgag acatctgcgc
      301 cgatagcagc aatcccttgc agataagcgt aatcggggtt tctgtcgcca cgttcgtagt
      361 taagttgcgt gtcttttttg acgccgcccg ccgccccgaa atcggcctga ttcagaccca
      421 attttttccg ctctgatttg agacgttccg aaaaatccga cattatcaaa ctttttttgt
      481 tgactagtgg acatttgtcc actatattca cattactttt tacgaataca aaccttacca
      541 catgagcaag tctcccaatt cgcgtttccg ctcatcttcg ctgcccgact cgcaggtggt
      601 catcttccgt atgaagaagg aagaaatcgc catcgctgac aggctggcca ggctggaccg
      661 ccgttcaaga tcgtcattca tccgtaacgt attgctgaaa ggcatcgctg agatcgagcg
      721 gcaccaacag cataaggccg cgtagggagt cagcatgtac gccgatccca aacgcctgcg
      781 cgacaaccgc cttacgctgc gcttcgacga ctatgaacat caactgatca tcgcgctggc
      841 gaattaccag ggcgagcagc catctacgct gctgcgccag atcgtcatgc gcgaagccac
      901 tgcgctgctg gatgaagcaa gcgtatcgcg cggtgacagc tgtgcgaagg ccgcaattcg
      961 ctgatggaga gcagacataa agatgccgga tcagcacgtg gccttcacgg aagaagagct
     1021 ggcccagctt gacagggtgc gccgtcagca agggctggac actgtccagc aggctgcaga
     1081 gtggcttgcc aagacccgcc tgcggcgtca ggcacgcaac atgacggggc gcggcagatc
     1141 gttgtacctc acagggaggg aggaaaaatg aatggtggtg tatcgatgaa atgcccctac
     1201 tgccaatcgc gcgcgatagc acgtaccagc aaggccatgt cgctgacgct gcgcgagatt
     1261 atttattcct gccgcaacca tgagtgcggc gccagctggg cagcctcact ggaagcgacg
     1321 cggctgctct cgccttcatc gatgccgaat ccgcatgtgc gcctgccgct gtcgccgcac
     1381 atccgggcag cggaactgat ttcgcaaatc gcctcgtcgc cgcagctaaa gcgcgacacg
     1441 caaccgccat aacatggcgc gcgcagcggc gccagcttaa tcaaccagtc agataccacc
     1501 agtgcctgcc atcaggcaca cggggttcgc acaccctgaa gcaaggcaaa acatgggaaa
     1561 caacatcgag atcaccgtca gatcagccgc caagaccgcg cacaaggatc gggccgtgca
     1621 agaccatccg cgcagtgcgc gtcttccagc agcgccggaa gttcaatttt ctggccggca
     1681 gacaggaagg gaaatgatcg tggccgcgcc ggtcgtcagc cgtgagcaga tgcagcgcgt
     1741 gcatgaagcg ctgcgcatca ccacgccact ggatgcatgc acgccaatga tgttgcgcac
     1801 gctggcatgc atcgcccact gctggcgcga tcgtatcccg gcgaatctct ggcgcgacct
     1861 ggacagctac cttgatccca caccgaacga atcctaccgg cagcaccaat tgcgctgccc
     1921 ggtcgagcgc gatcccgaat tgatcgatat caaacgccgc gctgctggcg acaacgactg
     1981 acccacatga atcaagattt acagcgcgat gtcctcgaac gtctgtccga attcaaattc
     2041 agaaaagagc agagaggcta cctgcgcggc ggcacctgtc catcctgcca caagaaggag
     2101 ttatacacgc atgccgatca tccatgggtg gtccgctgcg gccggttgaa tcactgcggc
     2161 tgggaaggcc acgtcaagga actgtatccg gacctgttcg aaaaatggtc ggagcgctat
     2221 ccgtcgacgc cggaaaacag gaatcccaac gccgccgccg atgcctacct gaagttcggc
     2281 cgtggctttg acctggcgaa gattcagggc gcctatacgc aggagcagta tttcgaccgc
     2341 gacctgcgca ttggcagcgt cacggtgcga ttttccatgg cgaacggtta ctgggaacgc
     2401 ctgatcgacc agccgcagcg tttcggcaag aagaaagctc gtttcagctt cggcctggac
     2461 taccaggggc tgtggtggca gtcggcgacg ctgcagctgc tgtcggtaaa ggagatctgg
     2521 atcgtcgagg gcatcttcga cgccatcgcg ctgatgcacc acggcatcgc cgccgtctca
     2581 gctatgtcgt ctagcaacta tccggaaaaa gcgctggccg atctggccgc atcgcgcggc
     2641 aacaaggatg tgacgctggt atgggcgctg gatggtgatg ccgccggccg cagcttcacc
     2701 ctgaagcatg tgcagcgcgc caccgaggca ggatggcaat gcaaggctgc gcagatcccg
     2761 cagactggca aaaacaagat cgactggaac gatgcacatc agcgtgaccg tctgcagcag
     2821 tcccatatcg atgcctacct ctactatgga tccttgctga tcgcaaagac agcggctgag
     2881 aaagcgctgc tgatttacaa caaggaaaac cggcacgagt tcgattttga atttcgccgc
     2941 cgcttgtatt ggttctcgct gaacatcgtt gaataccaga aggtacgcga cgcgatggac
     3001 aaggacggca ccgaagatga tgccctgccg gagcaaacca agcgtgagaa agcgcttgcc
     3061 gccagcatgt cgctgcgctc gatcgccaac tgccacccgc agccgcttta ctaccaggcc
     3121 aacatgatca ctgatgaatc ctggtactac tttcgcgtcg attttccgca cgacggcgca
     3181 gccatcaaga acaccttcac cgcttcgcaa ttgtccagcg cggcggaatt caagaagcgc
     3241 ctgctcggca ttgcgccggg cgcgctcttt accggcacca gccagatgct tgatcgcatc
     3301 attcaggaca agctggaaaa tatcaaacgc gtcgagacaa tcgactacat cggctacagc
     3361 atcgaccacg gatgctatgt gctaggcgat gtcgcggtga agggtggtgc tttgcacgac
     3421 ctcaacagcg aggattactt cgacatcggc cggctctcca tcaagtcgct caacaagtcg
     3481 gtatcgctgc ggatcaaccg cgaccgcagc gaatacacga ccgaatggct ggatttgctc
     3541 tggtgcgcct tcggcgccaa agggctggcc gcgctgacgt tctggttcgg atcgctgttt
     3601 gccgaacaga tccgcaaggc gcatagctca tatcccttcc ttgagatcgt cggcgatgcc
     3661 ggcgctggca agaccaccct gatcgaattg ctgtggaaac tcctcggccg cagcggctac
     3721 gaaggcttcg atcccagcaa gtccacgccg gcggcgcgcg cgcgcaattt ctcacaggta
     3781 tcgaacatgc cggtcgtgct gatcgaatcc gaccgggagc gcgtcgacgg cgaaaaatca
     3841 catatcaagt cgttcgactg ggatgagttg aagactgcct ataacggccg cagcatccgt
     3901 gcgcgtggca tggcaaccgg cggtaacgag acctacgagc cgccgttccg tggcgccgtc
     3961 gtcatcgcgc aaaacacgcc tgtagcggct tccgaggcca tcctgtcacg gatcgtccat
     4021 ctgtacttcg atcgcagcac acagacccca gcatcgggcg cagcggccga tgccatcaaa
     4081 tacctgacgg ccgagcaggt cagcggcttc gtcattgaag ccgcccgccg ggagaaagac
     4141 attctgcaat tggtgatcga gcgcacgccg atccatcaga aggaattgcg caccctgccg
     4201 aatatcaaga tgccgcgcat cgtcgagacg catgcgcaaa tgctggcgct ggccgatgca
     4261 ttggcgatgc tggtgaagct gccggcagac cggcatcagg cactgtgcgc acagatcatc
     4321 gacatgacta gcgaacggca aaccgtcatc aacggcgacc atcccatcgt gcaggaattc
     4381 tgggaagcct ttgacttcct gaacggcaat ccgaactcct gcctcgacca ctctgcagat
     4441 ccggcagtga tcgctgtcaa cctcaatcat ttcgtccagg tcgcatccga aaaacgccag
     4501 cagattccgc tactgcgcga cctgaagaaa gtcctgaaga caagcagcag gcgcaagtat
     4561 atcgaacaaa aagcggtgaa cagcacgatt taccgcaacg aattacccgt catgaacggc
     4621 aataaaccaa ccagcacgac cgtcaagtgc tgggtctttt ccaatcccga ctatccgaaa
     4681 ggagaccgat gaaattcctt ctgaaatacc gccattcacg cgccgccttg ctggtctgga
     4741 cgtcgctgat gttcgccact ggctatggca ttgcagtggc gaggctggca tgacgcgcat
     4801 cgcttgcttg ctccgcattc gcctgctgta tccccatttt cttttcaaga aaggttctcg
     4861 tatgcacatc ctgttcatga cctgcctatt ttgggtg
//
LOCUS       DASRDH010000310         2828 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1224611,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000310 DASRDH010000000
VERSION     DASRDH010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2828
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1224611"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="VFA14_07165"
     CDS             complement(<1..400)
                     /locus_tag="VFA14_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012435059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HZS81189.1"
                     /translation="MSIATTEEIVAELRAGRMVILVDEEDRENEGDLVLAADFVTPEA
                     INFMVKHARGLVCLTLTEEHCDRLDLPMMSTRNGTQFGTNFTVSIEAAEGVTTGISAA
                     DRARTIQVAVTKDATSSDIVQPGHIFPLRAS"
     gene            complement(487..1500)
                     /gene="dusA"
                     /locus_tag="VFA14_07170"
     CDS             complement(487..1500)
                     /gene="dusA"
                     /locus_tag="VFA14_07170"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HZS81190.1"
                     /translation="MSYGPRTVSVAPMMDWTDRHCRVFHRQITRHTWLYTEMVTTGAL
                     LHGDVSRHLDFNEAEHPVALQLGGSEPVDLAKSAKLGEQWGYDEINLNCGCPSERVQK
                     GAFGACLMAEPELVANCIKAMRDAVSIDVTVKHRIGVDDVASYAFVRDFVGRIAEAGS
                     KTFIVHARNAILKGLSTKENREIPPLRYSYVYRLKQDFPDLEILINGGIKTVTEIDRH
                     LQYVDGVMLGREVYHNPFLMAGFDARYYPMLAGGLQASRTQVIEAMMLYARRQLELHG
                     GGKGLRLNGITRHMLGLMAGMPGARAFRQILSDSRRLALGDPALLMEALSCMQHTQRD
                     TLK"
     gene            complement(1819..>2828)
                     /locus_tag="VFA14_07175"
     CDS             complement(1819..>2828)
                     /locus_tag="VFA14_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016836171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HZS81191.1"
                     /translation="IVLINGIEEISIGSTICEPDTPEGLPMLKIDEPTLTMNFMVNSS
                     PLAGREGKFVTTRQIRDRLERELKSNMALRVVQSENDDSTYEVSGRGELHLTILIENM
                     RREGYELAVSRPRVVFRMIDGVRHEPYENLTVDVEEASQGGVMEELGRRRGELQNMEP
                     DGKGRVRLEYRIPARGLIGFQGEFMTLTRGTGLMSHVFDDYAPVDNSKGELGGRRNGV
                     LISQDDGAAVAYAIWKLQDRGRMFVSHNDPVYEGMIIGIHSRDNDLVVNPIKGKQLTN
                     VRSSGTDEAVRLVPPIDMSLEYAVEFIDDDELVEITPKSIRLRKRFLKEHERKKASRE
                     A"
BASE COUNT          660 a          815 c          704 g          649 t
ORIGIN      
        1 tgctggcacg cagcgggaag atatgacctg gctgcacgat gtcggacgac gttgcgtctt
       61 tggtgaccgc cacctggata gtacgagcgc ggtcggcggc ggaaataccc gtcgtgacac
      121 cttcagcggc ttcgatcgac acagtgaaat tggtgccgaa ttgcgtgccg ttgcgcgtcg
      181 acatcatcgg caggtcgagc cgatcgcagt gctcctcggt cagcgtcaga cagaccaggc
      241 cgcgcgcatg cttgaccatg aaattgatgg cttcgggcgt gacaaaatca gcggccagca
      301 ccaaatcacc ttcattttca cgatcttctt catcgaccag gataaccatg cgtccggcgc
      361 gtagttcagc gacaatttct tctgttgttg cgatagacat aaaaaaattc tctcaatatc
      421 cccgtatttt aaaggatttg cggccggctt aatggctcat tggcagaact gcgctccggc
      481 actgctttat ttcagcgtgt cccgttgcgt gtgctgcatg cacgataacg cttccatcaa
      541 taaggccgga tcacccaagg ccagtcgtct ggaatccgac aaaatctgac ggaacgcgcg
      601 cgcgccaggc ataccggcca tcaaacccag catatggcgc gtgatgccgt tgagacgcag
      661 gcctttgccg ccaccatgca gttccagttg cctacgcgca tacagcatca ttgcttcaat
      721 cacctgggtg cggctggctt gcaggccacc ggctaacatc ggatagtagc gcgcatcgaa
      781 tccagccatc aggaaaggat tgtgatacac ctcgcgtccc aacatgacgc catcgacata
      841 ttgcagatgc cggtcgattt ccgtcacagt cttgatgccg ccgttgatga gaatttccag
      901 atccgggaaa tcctgcttga ggcggtagac gtagctgtat ctgagcggcg gaatctcgcg
      961 gttttccttt gtactcaatc ctttgaggat agcgttgcgg gcatggacga tgaaagtctt
     1021 gctgccggct tcggcaatcc tgccgacaaa atcgcgcacg aatgcatagg atgcgacatc
     1081 atcgacgcca atgcggtgct tgaccgtcac atcgatggaa acggcatcac gcatggcctt
     1141 gatgcaattg gcgaccagct ccggctcggc catcagacag gcgccaaacg cacccttctg
     1201 cacgcgctcg gacggacagc cacaattgag gttgatctcg tcataacccc attgctcgcc
     1261 cagtttagcg ctttttgcca aatcgacagg ttcgctgccg ccaagttgca aagcgactgg
     1321 atgttctgct tcattgaaat cgagatggcg cgacacatca ccgtgcaata acgcaccggt
     1381 cgtgaccatc tcggtataca gccaggtgtg gcgcgtgatc tgacgatgaa aaacgcggca
     1441 gtgccggtca gtccagtcca tcatgggggc gacgctgact gttctcggtc cgtaagacat
     1501 ataaaatagt attttgatat cgcagtagtg attttgcacc aagacatcgg gtatttcgca
     1561 atgcacaatc cccgtaccca gacaacagtc cggcaagaaa ttacctgaac acaaatacga
     1621 gataaaaaaa gtccgcctac cttgcggtgg cggacttaaa tccaatcctt tagaagggaa
     1681 ttggaggaga caggtatcac tatatcgcat cgcaccataa atgtccgctt tattctgtca
     1741 ataccagaca ttcatgaatt aaatattaat aaaccctgct cactgcctcc cacctaaatc
     1801 accggattgt tctgcctatt acgcttcgcg cgatgccttc ttgcgctcat gttccttcag
     1861 aaaacgcttg cgcagacgaa tgctctttgg cgtgatttcg accagttcat cgtcgtcgat
     1921 aaattcaacc gcatattcta gagacatgtc gatcggcggc accagacgca ctgcctcgtc
     1981 ggtacccgac gaacgcacgt tggtcaattg cttgcccttg atcggattga cgaccagatc
     2041 gttgtcgcgt gaatgaatac cgataatcat gccttcatac acagggtcgt tgtgactgac
     2101 gaacatacgg ccacgatcct gtaatttcca aatggcgtaa gcaacagcag caccgtcatc
     2161 ctgcgagatc agcacaccat tacggcggcc acccaactcg cccttgctgt tgtccactgg
     2221 cgcatagtcg tcgaacacat gactcatcag gccagtaccg cgcgtaagtg tcatgaattc
     2281 gccctgaaag ccaatcagac cacgcgcagg aatacggtac tctaggcgaa cacgtccctt
     2341 accgtccggt tccatatttt gcagctcacc acggcggcga cccagttctt ccatcacgcc
     2401 gccctggctg gcttcttcga cgtcaacggt cagattttca tacggctcgt ggcgcacgcc
     2461 gtcaatcatc ctgaacacga cgcgcggacg cgagacggcc agctcataac cttcacgacg
     2521 catgttttca atcaggatag tcagatgcaa ttcgccacgt cccgacactt cataagtcga
     2581 gtcgtcgttt tccgactgta ctacgcgcag cgccatgttg gacttcaatt cacgttccag
     2641 acgatcacga atctggcgtg tggtcacgaa tttgccctcg cggccggcca gcggcgaact
     2701 gttgaccatg aagttcatgg tcagcgtcgg ttcgtcgatc ttcaacatcg gcaggccctc
     2761 tggcgtatcc ggctcgcaga tggtagaacc gatgctgatt tcttcgatac cattgatcaa
     2821 cacgatgt
//
LOCUS       DASRDH010000311         5067 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1225199,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000311 DASRDH010000000
VERSION     DASRDH010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 5067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5067
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1225199"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..863)
                     /locus_tag="VFA14_07180"
     CDS             complement(105..863)
                     /locus_tag="VFA14_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006392057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HZS81192.1"
                     /translation="MDICAVRFYPAVQELIRLAERCGQPPAIVVTPNVDHVVRLDADA
                     GLRALYAQADYIFADGMPVVWASCMSEMSLPERVSGADLFVELLRVAVHRRVPVFIIG
                     GMPGQEQLLAAAFAQMYPGLQIALYCPSMQFDPLGAEAREALNRINACRPGLVFVCLG
                     MPKQERWALAHRDEMQAGVVLCVGAAMEFALGLKSRAPLWMQQAGLEWFWRLVSEPRR
                     LWRRYIVQGVRFIGVLRREWKHQHARRFRQSSGR"
     gene            1085..2038
                     /locus_tag="VFA14_07185"
     CDS             1085..2038
                     /locus_tag="VFA14_07185"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007882742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HZS81193.1"
                     /translation="MRNKRKQLLEPVEAPPAIDPRIVPLVVISPVRDESMYLRLTMDS
                     MVAQTFRPMEWILVDDGSTDATPDIVREYAVKYAFIRLVPRQNRGFRKLGGGVIAAFN
                     FGVEHIEHKDYEYIAKLDGDMSFGPLYLEHMFRKFDADPKLAAVSGKVYRDEDGRYIE
                     ESHIDEQVAGQFKLYRRTAFEDIGGFVQHLSWDGIDVHTAWMRGWTTFSYYHPQAWLW
                     HHRIMGSSDKHIYVGRLRWGRGNWYMGYHPLYAIATGINRMREKPKFIGGLLMIFGYF
                     WSCLRGLPRYENIEFRRYLRRWQLNRLKNLILGRKCPAPRA"
     gene            complement(2040..2435)
                     /locus_tag="VFA14_07190"
     CDS             complement(2040..2435)
                     /locus_tag="VFA14_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HZS81194.1"
                     /translation="MNERVNKPAVIVLPPIPAKRYFTIGEASGLCGVKPHVLRYWERE
                     FTQLKPVKRRGNRRYYQHHEVLLIRRIRELLYEQGFTINGARNKLGGCLTENQPEAIE
                     EIDASMDVEQIEAVRAGLTDILQLLQPRA"
     gene            complement(2454..2867)
                     /locus_tag="VFA14_07195"
     CDS             complement(2454..2867)
                     /locus_tag="VFA14_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008113572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HZS81195.1"
                     /translation="MNNVNSRELRSVLDVDLQRAVLESRMRSQAEKNLPTLTKAELAE
                     LLFEQVGLNKREAKDMIETFFGEIRNALERGEAVKLSGFGNFQLRDKPQRPGRNPKTG
                     EEILITARRVVTFHASQKLKEMVEVSGGRPLDIAV"
     gene            complement(2916..>5067)
                     /gene="pheT"
                     /locus_tag="VFA14_07200"
     CDS             complement(2916..>5067)
                     /gene="pheT"
                     /locus_tag="VFA14_07200"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HZS81196.1"
                     /translation="AMAGAVLPPDADGKPLEIEVSQLRGVESQGMLCSARELKLPEDH
                     GGLLELPDDAPVGQNFRDYFLLNDLKFTIKLTPNRADCLSVFGVAREIAALTDTPLKP
                     PACQKFASNSDEKLPVRISAPDLCGRFAGRVIRGLNARAATPQWMKQRLERSGQRPVS
                     ALVDISNYVMLELGRPSHVFDLDKIHGGLDVRWGRKGEQIKLLNGNTVDVDEWVGVIA
                     DDKGPESLAGIMGGDATAVSLDTSNIYLEAAFWFPQSIQGRARRYNFATDAAHRFERG
                     VDFATVVEHIERITALLIEICGTPGAKVGPIDDQIVNLPRREVVEVRTARAARIIGVD
                     ISDELIANVFTRLGLPFTQEPGLFAVTSPSYRFDLEIEEDLIEEIARVYGFENIPALP
                     PVAVNTMKNAPENIRSLFAIRHQLADLDYQEVINFSFVDSAWEADFAGNQTPIKLLNP
                     IASQMSVMRSSLIAGLIANLRYNINRKVGRVRVFEVGTVFQRDAAVQNGPLEVAGYHQ
                     PKRVAALAYGPVADEQWGQETRTVDFFDLKADLEALFAPRVLRFVRAGNPALHPGRSA
                     QVELDGRNIGLIGELHPRLQQQYDLPLAPVVFEVDAVALQERHVPVYEEISRFPTVTR
                     DLALVVKQAVQVQDLLDIFAAEKQGNAACAVIQTIVLFDEYQGKGLESDEKSLAFRFT
                     LQDTRNTLRDETVDAAMAAIVAVATVGLAARLRA"
BASE COUNT         1170 a         1498 c         1358 g         1041 t
ORIGIN      
        1 caaagctcca gcgtcagaac tatcgtcaag gcataggctg cgccgaccgc aagtccaagc
       61 aaagcgatgt ggtgagtggg cctgtcccgg taagagatca atggctacct ccctgagctc
      121 tgcctgaacc tgcgtgcatg ctgatgcttc cattcgcggc gcagcacacc gatgaagcgc
      181 acgccttgca cgatgtatct gcgccacaag cgacgcggct ccgataccag ccgccagaac
      241 cactccagtc ccgcctgctg catccacagc ggcgcgcgcg atttcaaacc gagtgcaaac
      301 tccatggccg cacccacgca cagcacgacg ccagcctgca tctcgtcgcg gtgcgccaag
      361 gcccagcgct cctgcttggg catgcccaga caaacgaata ccaggcccgg ccggcacgca
      421 ttgatacgat tcaaggcttc tcgagcttcc gcgccgagcg gatcgaactg catcgacgga
      481 caatacagcg cgatctgcaa gcccggatac atctgcgcga aagcagcggc cagcagctgc
      541 tcctgtcccg gcataccgcc aatgatgaat accggcaccc gccggtgtac cgccacacgc
      601 agcagttcca caaataaatc agcgccgctt acccgctccg gcagtgacat ttcactcatg
      661 caactggccc agaccacagg cataccgtcg gcaaaaatgt agtcggcctg agcatacaag
      721 gcgcgcaagc cggcgtccgc atccagtcgt accacatgat ccacattagg cgtgacaacg
      781 atcgcaggcg gctgcccgca ccgctcggcc aggcggatca attcctgcac cgccggatag
      841 aaacgcacag cgcaaatgtc caacccaaac aggctagacg tgggaaacgc acactgtttc
      901 ttcatccgtt ccgacactgg aaatatcgaa ttaaatacga atttaattat ctatattttt
      961 aaatataaat agaaaaattt caatgtcata tgtttgacat gcgtttttta agcaatcgta
     1021 acttgacaga acagggtgaa cgatataagc atataacccc acacgacaga tgcaagaaag
     1081 acacatgcga aacaagcgga aacagttgct ggagccggta gaagcacctc ccgccatcga
     1141 tccgcgtatc gtcccactgg tggtcatttc gccagtgcgc gacgagtcga tgtatctgag
     1201 gctgaccatg gactccatgg tagcccagac ttttcgtcct atggagtgga ttctggtcga
     1261 cgacggttct accgatgcca cgcctgacat cgtgcgcgaa tatgccgtga aatatgcatt
     1321 tatccgtttg gtgccgcgcc agaatcgcgg cttccgcaag ctcggcggcg gcgtcattgc
     1381 cgccttcaac ttcggcgtcg aacacatcga acacaaagac tacgaataca ttgccaaact
     1441 cgatggtgac atgtctttcg gcccactcta tctggaacac atgttccgta aattcgacgc
     1501 agaccccaag ctagccgccg tatccggcaa agtctatcgt gatgaagacg gccggtacat
     1561 cgaagaaagc catatcgacg agcaagtcgc cggccagttc aaactgtaca ggcgcactgc
     1621 ctttgaagac atcggcggct tcgttcagca tttatcatgg gatggcatcg acgtacacac
     1681 ggcatggatg cgggggtgga caacctttag ctattaccat ccgcaagcct ggctctggca
     1741 ccatcgtatc atgggatcat ccgacaaaca tatttatgta ggaaggctgc gctggggccg
     1801 cggcaactgg tacatgggct atcacccgct gtacgccatc gccaccggca tcaaccgcat
     1861 gcgtgaaaaa ccgaagttca ttggcggatt gctgatgatt ttcggttatt tctggtcgtg
     1921 tctgcgcggc ttgccgcgtt acgaaaacat cgaatttcga cgctatctgc gacgctggca
     1981 gctaaaccgc ctgaaaaacc tcatcctggg tagaaaatgc cccgctccca gagcgtgaac
     2041 taagctcgcg gctggagcaa ttgcagaatg tcggtgagtc cggcgcgcac tgcttcgatt
     2101 tgctcgacgt ccatcgaggc atcgatttct tcgatggctt ccggctgatt ttcagtaagg
     2161 cagccaccca gcttgttgcg ggcgccgttg atagtgaagc cctgttcgta cagcagctcg
     2221 cggatgcggc gaatcagtaa tacttcatga tgctgataat aacggcggtt gccacggcgt
     2281 ttgaccggct tgagctgagt gaattcccgc tcccagtagc gcagcacatg cggcttgacg
     2341 ccgcacaatc cgctggcttc gccaatggtg aaatagcgct tggcgggaat cggcggcaaa
     2401 acgattaccg caggtttgtt gacgcgttca ttcatggagc gggatgatgg cgattacaca
     2461 gcgatgtcaa gaggacgacc gccagacact tcgaccattt ccttgagctt ctggctggcg
     2521 tggaaagtga cgacacggcg cgcggtgatg agaatctctt ctccagtttt cggattgcgg
     2581 ccgggacgct gcggcttgtc gcgcaactgg aaattaccga agccggacag tttgacagcc
     2641 tcaccacgct ccagagcatt acgaatttca ccgaagaaag tctcaatcat gtcttttgct
     2701 tcacgcttgt tgagaccaac ctgctcaaat aaaagttcgg caagttctgc cttggtcagc
     2761 gtcggcagat ttttttccgc ctgcgagcgc atccgggatt ccagtacggc gcgctgcaag
     2821 tcgacatcca gcacagatcg aagctctctt gaattcacat tgttcatcat aggtaagtcg
     2881 ccccttttag aacatgttca cgcactgatt catcgctatg cacgcaatct ggcagcgagt
     2941 ccaactgtcg cgacagccac gatggcagcc attgcagcgt ctactgtctc atcccggagt
     3001 gtgtttcgag tatcttgcaa ggtgaaccgg aaagcaagac ttttttcgtc gctctccaag
     3061 ccctttcctt gatattcatc aaataaaaca atggtttgga tgaccgcaca ggcagcgttc
     3121 ccctgcttct ccgcagcaaa aatatcgagc agatcctgga cctgcacagc ttgtttgacg
     3181 accagcgcga ggtcgcgcgt gaccgtcggg aacctggaaa tttcctcata gaccggcaca
     3241 tgacgttcct gcagcgcgac ggcatcaact tcaaatacca ccggcgccaa tggcaaatcg
     3301 tattgctgtt gcaaacgcgg atgtagttcg ccgatcaggc cgatattcct gccatccagc
     3361 tcgacctgcg ccgaacgtcc cggatgcagc gccggattcc cggcgcggac aaaacgcaaa
     3421 acacgcggcg cgaacagggc ttccagatcc gccttgaggt cgaagaaatc gacagtgcgc
     3481 gtctcctgcc cccactgctc gtcagccacc ggaccataag ccagcgcggc gacacgcttg
     3541 ggttgatgat agccggccac ttccagaggg ccattttgca cggcagcgtc gcgctggaac
     3601 actgtaccga cttcgaatac acgcacgcgg ccgaccttgc gattgatgtt ataacgcagg
     3661 ttggcgatca ggccggcgat caatgaggag cgcatcacac tcatctggct ggcaatcgga
     3721 ttcagcagct tgatcggcgt ctgattgcca gcgaagtcag cctcccatgc actatcgacg
     3781 aagctgaaat tgatgacttc ctggtagtcg agatcagcca gttgatgacg aatggcaaac
     3841 agcgaacgga tgttttccgg cgcattcttc atcgtgttga cggccaccgg cggcaaagcc
     3901 gggatatttt caaaaccgta gacgcgggcg atttcttcga tcaggtcttc ttcgatttcg
     3961 agatcgaaac gataagacgg tgacgtcaca gcgaacaagc ctggttcttg cgtgaacggt
     4021 agaccaagac gggtgaaaac gttggcgatc agttcgtcgc tgatatccac gccgatgatc
     4081 ctggcggcgc gtgcggtacg tacctcgacc acctcgcgcc tgggcagatt gacgatctga
     4141 tcgtcgatgg gaccgacctt ggcgccaggc gtgccgcaga tttcgatcag caaggcggta
     4201 atacgctcga tgtgctcgac cacagtggcg aaatcgacgc cgcgctcgaa acgatgcgca
     4261 gcatcggttg cgaaattgta gcggcgcgca cgaccctgga tcgactgcgg gaaccagaac
     4321 gcggcttcca gatagatgtt gctggtatcg agcgagacgg cagtggcgtc gccgcccatg
     4381 atgccggcca gcgattcggg gcctttgtca tcagcgatca cacccaccca ttcatcgacg
     4441 tccacggtat tgccattcaa gagcttgatc tgctcacctt tcctgcccca gcgcacgtcg
     4501 aggccgccgt ggattttatc cagatcgaat acgtgcgaag gacggccgag ttccagcatg
     4561 acgtagttgg aaatatcgac cagcgccgaa accggacgct gaccgctgcg ctccaggcgc
     4621 tgcttcatcc attgcggcgt tgctgccctg gcattcaggc cacggatgac acggccggca
     4681 aaacggccgc acagatcagg ggcggaaatc ctgaccggca atttttcatc actattgcta
     4741 gcaaacttct gacaggccgg cggcttgagc ggcgtatcgg tcagcgccgc gatttcacgc
     4801 gccacaccga acactgacag acaatcggcc ctgttgggcg tgagcttgat ggtgaacttg
     4861 aggtcgttga gcaaaaaata gtcacggaaa ttctggccga ccggcgcatc atccggcaat
     4921 tccagcaagc cgccatgatc ttccggcagc ttcagttcac gcgcggaaca cagcatacct
     4981 tgcgactcga cgccgcgcag ctggctgacc tcgatctcaa gcggcttgcc atcggcgtcc
     5041 ggcggcagca cggcgccagc catcgcg
//
LOCUS       DASRDH010000312         6404 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1334235,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000312 DASRDH010000000
VERSION     DASRDH010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 6404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6404
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1334235"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..1521)
                     /locus_tag="VFA14_07205"
     CDS             complement(73..1521)
                     /locus_tag="VFA14_07205"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006465328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HZS81197.1"
                     /translation="MYNDVQLFINGRWTASVSGRTIPVVNPATEEVIGRVACANEQDL
                     DAALASAQTGFAAWREVSAFERSRIMRKAADILRERTDDVARLMTQEQGKPLAEARME
                     TLGAADTIDWFAEEARRTYGRLIPARVPNAYQMVIKEPVGPVAAFTPWNFPLNQVVRK
                     LSAALAAGCSIIVKAPEETPASPAALIRAFADASIPEGVVNLVYGVPSEISTYLISHP
                     VIKKISFTGSTPVGKQLAALAGQYMKRATMELGGHAPAIVFNDADIDAAVRQLALAKF
                     RNAGQVCVSPTRFLVQKNVFDAFVDKFVTAAGLLKVGDGMAASTTMGPLANKRRIPAL
                     EALISDAVEKGAQLRLGGKRLGGKRLGGKGYFFEPTVLTDVPLSARVMNEEPFGPLVP
                     INSFDTLDEVIAEANRLPFGLAAYAYAKSAHTKHALSSRIESGMLTINHLGLALPEVP
                     FGGIKDSGYGSEGGAEAIEAYVNTKFVSQLDV"
     gene            complement(1610..2728)
                     /locus_tag="VFA14_07210"
     CDS             complement(1610..2728)
                     /locus_tag="VFA14_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007880323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HZS81198.1"
                     /translation="MKSKLFSPLKLRELTLPNRIVVSPMCQYSAQDGFANDWHLVHLG
                     SRAVGGAGLVIVEAAGVLAEGRITPEDIGIWKDEHIGPLRRITRFIETQGSRAGIQLA
                     HAGRKASAYRPWADKDGSIPAAEGGWETVGPSAISFDGTFRTPAALSIAGIAQRVQAF
                     ADAALRAHEAGFKVVEIHAAHGYLLHEFLSPLSNQRSDQYGGALENRVRFLMEVVTAV
                     RKVWPADLPLFVRVSATDWAEGGLTPDEIVAVSRLLRDAGVDLMDISSGGNIAAATIP
                     SGPGYQTAFAARVRCESNIATGTVGMITNAMQAEHVLRTEQADLVLLGRELLRDPYWP
                     LHAADELRENVAWPPQYARSAASSKTPLRENVDYSDLD"
     gene            complement(2728..4491)
                     /gene="leuA"
                     /locus_tag="VFA14_07215"
     CDS             complement(2728..4491)
                     /gene="leuA"
                     /locus_tag="VFA14_07215"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007880327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HZS81199.1"
                     /translation="MMSSNPAAKYRAFPPIPLSDRTWPNQVISRPPIWMSTDLRDGNQ
                     ALIEPMNTERKLRFFDMLLQTGLKEIEVGFPSASQTDFDFVRKLIEENRVPDDVTIIA
                     LTQSRDELIHRTIDSLQGAKQAIVHLYNSVAPAFRKIVFNMSREQIKEIAVAGTRLVR
                     ELTDARPETQWRYEYSPESFSTTELDFSREICDAVCASWGATPERKVILNLPSTVESS
                     TPNVYADQIEWMHRNLQYRAASIISVHPHNDRGTAVASAELAIMAGAERVEGCLFGNG
                     ERTGNVDLVTLALNLYTQGIHPGLDFSDIDTVRQCVEECNQLPVHPRHPYVGDLVFTA
                     FSGSHQDAIKKGFARQQTDAIWEVPYLPIDPADLGRSYDAVIRVNSQSGKGGMAYLLE
                     QEYGLSLPRRLQIEFSRVIQREADVTGKEIVASDIYTIFKSEYLDRDAPYVYRAHRMS
                     EDSSRRESIRIDIDIECDGEKFTLHGIGNGPIDSFVNALGLDIKLMDFHEHAIDAGAN
                     AQAASYIELRLNEAPTGFGVGIDTNIVTASFKAILSAINRQIAIDHTNQLSLLKAWKE
                     SRRGDSSIQSAVFHDVCNQEV"
     gene            complement(4835..6022)
                     /gene="ftsZ"
                     /locus_tag="VFA14_07220"
     CDS             complement(4835..6022)
                     /gene="ftsZ"
                     /locus_tag="VFA14_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008116405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HZS81200.1"
                     /translation="MEIDMVDNATLGTIIKVVGIGGAGGNAVQHMINKGVSGVEFIAA
                     NTDAQALKQSRADNIIQIGESGLGAGMQPEVGRRLAEETRERIEDALRGAHMVFIAAG
                     MGGGTGTGAAPVVAQIAKELGALTVAVVSKPFSYEGQKCMDIADQGLEELTQHVDSLI
                     VILNEKLEEIYEDGSMIEWLGHADDVLNNAVAGIAEIINVPGHINVDFNDVKTIMGEQ
                     GKAMMGTATASGVDRARVAAEQAVASPLLDGIDLSGARGVLVNVTASRNLKGKEIKEV
                     MGTVRAFAAADASIAQGIAYDDNMGDDIRVTVVATGLGRARKSVQLVQTLRTGTHSES
                     MMAGLGAFSGGNGGQPAGAGFGGMKAPAVWRRESASDTVRALEKNGMETYDIPAFLRK
                     QAD"
     gene            complement(6113..>6404)
                     /locus_tag="VFA14_07225"
     CDS             complement(6113..>6404)
                     /locus_tag="VFA14_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014898179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HZS81201.1"
                     /translation="GYEEVLSSGIVLTGGSVMMPGMVELAEDIFLKPARIGVPEYSGQ
                     LADVVRSPRYSTVLGLLLGAKKQYLRGYLVTCQEGSVKAAWQRMKEWFLGNF"
BASE COUNT         1327 a         1861 c         1837 g         1379 t
ORIGIN      
        1 gtaaaaatcc caaagcctgg atttaatgtc caactctttg ggatcatttt ttgtttacac
       61 tgaaaccagc gcttacacgt ccagttgcga cacaaacttg gtattgacat aggcttcgat
      121 ggcttcggcg ccgccttcgg aaccgtagcc actatccttg atgccgccga acggcacttc
      181 cggcagcgcc agacccagat gattgatggt gagcatgccg ctttcgatac ggctcgataa
      241 cgcatgtttg gtatgcgccg atttagcgta ggcataggcc gccagaccaa agggcagacg
      301 gttggcttcg gcgatgactt cgtcgagcgt gtcgaaggaa ttgatcggca ccagcgggcc
      361 gaaaggttct tcgttcatca cgcgtgccga cagcggcaca tcggtcaata cggtcggttc
      421 gaagaaatag cccttgccgc ccaggcgctt gccgcccagg cgcttgccgc ccaggcgcaa
      481 ttgcgcgccc ttctcgacgg catcgctgat cagtgcttcc agcgccggga tgcggcgctt
      541 gttggcaagc ggacccatcg tcgtactggc tgccatgcca tcgccgactt tgagcaggcc
      601 ggcggcagtg acgaatttat cgacgaaggc atcgaatacg tttttctgca ccaggaaacg
      661 ggtcggcgag acacagacct gaccggcgtt gcggaacttg gccaacgcca gttgcctgac
      721 agcggcgtcg atgtcggcgt cgttgaacac gatggctggc gcgtgaccgc ccagttccat
      781 cgttgcgcgc ttcatatact ggccggccag cgccgccagt tgtttaccta caggtgtcga
      841 gccggtgaag gaaatctttt tgatgaccgg atgcgaaatc aaataggtcg aaatttccga
      901 cgggacaccg tagaccaggt tgaccacgcc ttcaggaatg ctggcatcag caaatgcgcg
      961 gatcaatgca gcaggcgaag ccggggtttc ttccggcgcc ttgacgatga tggaacagcc
     1021 ggcggccagc gcggcagaca gcttgcgcac gacctgattc aatgggaagt tccacggtgt
     1081 gaacgcagcg accggaccga ccggttcctt gatcaccatt tggtacgcgt tcggcacgcg
     1141 cgcggggatg agacgaccat aagtacgacg cgcttcttcg gcgaaccaat cgatggtgtc
     1201 ggcagcgccc agggtttcca ttcttgcttc ggccagcggc ttgccttgtt cctgcgtcat
     1261 cagtcgcgcc acgtcatcgg tacgctcgcg cagaatatcg gcggccttgc gcatgattct
     1321 ggaacgctcg aacgcagata cctcgcgcca ggcggcgaaa cctgtttgcg ccgatgccag
     1381 cgcggcatcg agatcctgct cgttggcgca ggcgacgcgg ccgatgactt cttcggtcgc
     1441 aggattgacg accgggatgg tacgcccgga gacgctggca gtccagcgac cattgatgaa
     1501 gagttgcaca tcgttgtaca tgaggtggat ctccttacaa atctgatgga tcaaataccg
     1561 tgcgtcaacg aaacgccgga catattgttt atatccggtg gatcaacgat cagtccagat
     1621 cgctgtaatc gacgttctcg cgcagtggcg tcttgctgga ggcggcgctg cgcgcatatt
     1681 gcggcggcca ggcgacattt tccctgagtt catcggcggc atgcaagggc cagtaaggat
     1741 cgcgcagcag ttcgcgcccc aataatacaa gatcagcctg ctcggtgcgc aggacgtgct
     1801 ctgcctgcat tgcattggtg atcatgccga ccgtgccggt ggcgatgttg ctttcgcacc
     1861 tgacgcgtgc ggcaaaagca gtctgatagc cgggacctga gggaatcgtc gcagcggcga
     1921 tattgccgcc gctggagatg tccatgaggt cgacgccggc gtcgcgcagc aggcgtgaaa
     1981 cggcaacgat ctcatccggc gtcagaccgc cctcagccca gtcagtcgcc gatacgcgca
     2041 cgaacaaggg cagatccgcc ggccagacct tgcgcacggc cgtaacgact tccatcagga
     2101 aacgcacgcg attttccagc gcgccgccgt attgatcgct gcgttgattg ctcaacggtg
     2161 acagaaattc atgcagcagg taaccatgcg cagcatggat ttcgaccact ttgaaaccag
     2221 cttcgtgcgc gcgcagcgcc gcatcggcga aagcttgcac acgctgtgca atgcccgcga
     2281 tgctcagcgc tgccggtgtc ctgaatgtgc catcgaaaga aatcgccgac gggccgacgg
     2341 tttcccagcc gccttctgca gcgggaatgc tgccgtcctt gtccgcccat gggcggtagg
     2401 cgctggcctt gcggccggca tgcgccagct gtatgccagc gcgcgatccc tgtgtttcaa
     2461 tgaagcgggt aatgcggcgt agcggcccga tgtgttcgtc tttccagata ccgatatctt
     2521 ccggcgtgat acggccttcg gccagcacgc cggcagcttc gacgatcacc agcccagcgc
     2581 cgccgacggc acggctgccg aggtgaacca ggtgccagtc gttggcgaaa ccatcctgcg
     2641 ccgaatactg gcacattggt gaaacgacaa tgcggttcgg cagggtcagc tcgcgtaatt
     2701 ttaacgggga aaacaactta ctcttcatta aacctcctgg ttacatacgt cgtgaaaaac
     2761 cgccgactgg atggaactgt ccccccggcg gctttccttc catgctttga gcaaggaaag
     2821 ctgattggtg tggtcgatcg cgatctggcg attgatggcg ctgaggatgg ctttgaatga
     2881 tgccgtaacg atgttggtat cgatgccaac gccaaagcct gtcggcgctt cgtttagacg
     2941 cagttcgatg tagctggctg cttgcgcatt ggcaccagcg tcgatggcat gttcatggaa
     3001 atccatcagc ttgatgtcta gtcccagcgc attgacgaag gagtcgatcg gaccgttacc
     3061 gatgccatgc aaggtgaact tctcgccgtc gcactcaatg tcgatgtcga tcctgatcga
     3121 ttccctgcgg gaagaatcct ccgacatgcg gtgcgcgcgg tagacatacg gtgcatcgcg
     3181 gtccagatat tcgctcttga agatggtata gatatcgctg gcgacgattt ccttgccagt
     3241 gacgtccgct tcgcgctgaa tgacacggct gaattcgatc tgtaggcggc gcggcagaga
     3301 cagaccgtac tcctgttcca gcaggtaagc catgccgcct ttgcctgact ggctattgac
     3361 gcgaatcaca gcatcatagc tgcgacccag gtcagccggg tcgatcggca gataaggcac
     3421 ttcccagatg gcatcggtct gctgcctggc gaagcccttc ttgatcgcat cttgatgcga
     3481 tccggagaaa gcggtgaaaa ccagatcgcc gacataggga tggcgcggat ggaccgggag
     3541 ctgattgcat tcttcaacgc attgacgcac ggtgtcgata tcagaaaaat ccaggccagg
     3601 atggatgccc tgggtataca ggttcagggc cagtgtgacc agatcgacgt tgccagtacg
     3661 ttcgccattg ccgaacaggc agccttcgac gcgctcggcg ccggccatga tagccagttc
     3721 ggcggaagcg acggcagtac cacggtcatt atgcgggtga accgagatga tggatgcggc
     3781 gcggtattgc aggttgcggt gcatccattc gatctggtcg gcataaacgt tcggggtgct
     3841 gctttcaacg gtcgaaggca ggttcaggat gactttgcgt tccggcgtgg cgccccagct
     3901 tgcacagacg gcatcgcaga tttcacgtga gaagtcgagc tcagtggtgc tgaaggattc
     3961 tggcgaatac tcatagcgcc attgggtttc cggacgggca tcggtcagtt ccctgaccag
     4021 gcgggtaccg gcgacagcga tttccttgat ctgctcgcgg ctcatgttga agacgatctt
     4081 gcggaaggcc ggcgcgactg agttatacag atgcacgata gcttgcttgg cgccttgcag
     4141 cgagtcgatg gtacggtgga tcaactcatc gcgcgactgg gtcagggcga tgatggtgac
     4201 gtcgtcggga acacggtttt cttcgatcag cttgcgcacg aagtcaaaat cggtttgcga
     4261 tgcggaaggg aaaccgacct cgatttcctt caggccggtt tgcagcagca tgtcaaaaaa
     4321 gcgcagttta cgctccgtgt tcatgggttc gatcagcgcc tgattgccat cgcgcaggtc
     4381 ggtactcatc cagatcggtg gccgggaaat gacttggttc ggccaggtac gatcagagag
     4441 cggaatcggc gggaacgcac ggtactttgc tgccgggttg cttgacatca tggtaaggcc
     4501 tctttgattt tgtcactgct ttgcaatgga gaaacgggaa agtccatccg atgtggcgaa
     4561 gaggctgcct gactgccact aacgcaaggc caggcaaccg atcagtagca gtagtcgtgc
     4621 cggtacatgc gcaaaggcca tcatggcagc ccgtgcgcag gaatgcaaag atagtgtgat
     4681 ggtcttgata gacatgagca acttttcgag aaaaactgac caaatcattg gcagggctat
     4741 ggccaattct atttgatttc ttccctgcat gcatcgacgg gcaatggcac cgttatctcg
     4801 gggccgcctg tttgcaggaa tgctgtacta ctaactagtc agcctgcttg cgcaggaaag
     4861 ccggaatgtc ataagtctcc ataccattct tttccagcgc acgcacggtg tcggaagccg
     4921 attcgcggcg ccacactgcc ggcgccttca tgccgccgaa tcccgcacca gccggctgac
     4981 cgccattacc gccgctgaat gcgccgagtc cggccatcat cgattcgctg tgcgtaccag
     5041 tacgcaaggt ctgtaccaat tgcaccgact tgcgggcacg acccagaccg gttgccacga
     5101 ccgtcacgcg gatgtcgtcg cccatattgt cgtcgtaggc gatgccttgc gcaatcgatg
     5161 catcggcggc agcgaaagca cgcacggttc ccatgacttc cttgatttcc ttacccttca
     5221 gattacggct ggcggtcaca ttgaccaaca cgccgcgcgc accggacagg tcgataccat
     5281 cgagcagcgg cgacgccact gcctgctccg ccgccacacg ggcacggtcc acgccggaag
     5341 cggtcgccgt acccatcatg gccttgcctt gctcgcccat gatggtcttg acgtcgttga
     5401 agtcgacatt gatatgacct ggtacattga tgatttcggc gataccggca acggcgttgt
     5461 tgagtacgtc gtccgcgtga cccaaccatt cgatcatgct gccgtcttcg tagatttctt
     5521 ccagtttttc attcaggatg acgatcagtg aatcgacatg ctgcgtcagt tcttccagac
     5581 cctggtcagc gatatccatg cacttctggc cttcatagga aaacggcttg gaaaccacag
     5641 ccacggtcag cgcgcccaat tccttggcga tctgcgccac caccggcgct gcgccggtac
     5701 cggtgccgcc gcccataccc gcggcaatga ataccatgtg cgcgccgcgc agcgcatctt
     5761 cgatacgttc acgggtttct tcggccagac ggcgaccgac ctccggctgc atgcccgcgc
     5821 ccagaccact ttcaccgatc tggatgatgt tgtccgccct ggattgtttc aatgcctgcg
     5881 catctgtatt tgctgcaatg aactcaacgc cggacacgcc cttgttgatc atatgctgca
     5941 ccgcattgcc acctgcgcca ccgatgccca cgactttgat gatcgtaccg agagtggcat
     6001 tgtcaaccat atctatttcc atgatgactc cttctaattg tatgttgtac aacctgtagt
     6061 tgatgaactg gatttcaaaa acgatgcatg tacagataaa gcaaacgact acttaaaaat
     6121 tacctaaaaa ccattccttc atacgctgcc aggccgcttt gaccgaacct tcctgacagg
     6181 tgacgaggta tccgcgcaga tactgctttt tcgctcccag caacaagccc agtaccgtcg
     6241 aataacgtgg actgcgtacc acatcagcca gctgcccgct atattccggc acccctatcc
     6301 gcgccggctt gaggaagata tcctctgcca gttcaaccat tcccggcatc atcacagagc
     6361 cgccggtgag tacgatgccc gacgacagca cctcctcata accc
//
LOCUS       DASRDH010000313         2100 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_306090,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000313 DASRDH010000000
VERSION     DASRDH010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306090"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="VFA14_07230"
     CDS             <1..573
                     /locus_tag="VFA14_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007881097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HZS81202.1"
                     /translation="VWAFGYHNAARIRHAGTVDAARLSDALKTLRREVHKILQQADHD
                     LKRIQYNTVVSACMKMLNTLEAASPEDSPTVNAVLAECASIFLRVLNPVAPHITHVLW
                     QELGFAERHGDILDADWPQVDAAALEQSEIDMMVQVNGKLRGSITITRDADKAAIETL
                     ALANESVRKFLTGAPKKIIVVPGKLINIVV"
     gene            599..1129
                     /gene="lptE"
                     /locus_tag="VFA14_07235"
     CDS             599..1129
                     /gene="lptE"
                     /locus_tag="VFA14_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HZS81203.1"
                     /translation="MPFPLYQRQVLPRLLVLLAVLMLSACGFHLRGSVNLPFRTIYLG
                     FAGDSSLGAELKRSLRTGGVKVVDQVAEAEAVMQVLADTREKKILSLGTNGRVREYSL
                     YQRFRFQIKDQAGKILVAPTDIALKRDVSYDASQELAKQSEETLLYRDMQSDLVQQIL
                     RRLSAAKPGSVEPIKE"
     gene            1133..>2100
                     /gene="holA"
                     /locus_tag="VFA14_07240"
     CDS             1133..>2100
                     /gene="holA"
                     /locus_tag="VFA14_07240"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013232630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HZS81204.1"
                     /translation="MQLRLDALDMHLARSLSPLYIIASDEHLLALEAAARIRMAARAN
                     GYTEREVLVVERSFKWGELLAANQSQSLFGDKKLIELRMPTGKPGKDGGQVLQDYAAR
                     LNSDHLTIISLPRLDVATAKTAWVGALRQAGIYIDIPLVERAQLPDWIGSRLTSQQQN
                     ADRPCLDFIADLVEGNLLAAYQEIQKLALLYPAGKLTFEQIHAAVLNVARYDVFKLNE
                     AILSGDTARLMRMMDGLKGEGAALPLVLWAVTEEVRTLLKLKSSVTQGKQLGILLKEH
                     RIWGPREKLMEPALRRISLSMLQTALREAAQIDRMVKGLRAQAFSG"
BASE COUNT          476 a          632 c          602 g          390 t
ORIGIN      
        1 gtctgggcgt tcggctatca taatgcggcg cgcatccggc acgccggcac agtcgatgct
       61 gcccggctga gcgatgcgct caagaccttg cgccgcgaag tacataaaat cctgcaacag
      121 gccgatcacg acctgaaacg catccagtac aacaccgtgg tgtcggcctg catgaaaatg
      181 ctcaacacgc tcgaagcggc cagtccggaa gacagcccga ctgtcaacgc cgtgcttgcc
      241 gaatgcgcat cgatcttcct gcgcgtcctc aatccggtcg cgccgcatat cacgcatgta
      301 ctgtggcagg aactcggctt cgccgaacgg catggtgaca tcctcgacgc cgactggccg
      361 caggtcgatg ccgccgcgct ggagcaaagc gagatcgaca tgatggtcca ggtcaatggc
      421 aaactgcgcg gcagtatcac catcaccaga gatgccgaca aggcagcgat cgaaacgctg
      481 gcgctagcca atgaaagcgt gcgcaagttc ctcaccggcg cgccgaagaa aatcatcgtc
      541 gtacccggca agctgatcaa catcgttgtc tgaacctgaa tcgaccgcag acgccgccat
      601 gccatttcca ctttaccaac gccaggtatt gccacgcttg ctggtgctgc tggcggtgct
      661 catgctcagc gcctgcggct ttcacctgcg cgggtcggtg aatttaccgt tcagaacgat
      721 ttacctcggc tttgccggcg actcctcgct gggcgcggaa ttaaagcgct ctctgcgcac
      781 aggcggcgta aaggtagtgg atcaggtggc tgaggcagaa gccgtgatgc aagtgctggc
      841 cgatacgcgc gaaaagaaga tcctgtcgct tggcaccaat ggccgagtac gcgaatacag
      901 tttgtatcaa cgctttcgtt ttcagatcaa ggatcaggct ggcaagatac tggtcgcgcc
      961 caccgacatc gcgctcaagc gcgatgtcag ctatgacgcc agtcaggaac tggccaagca
     1021 gtcggaagaa acgctactct accgcgatat gcaaagcgat ttggtacagc agatcctgcg
     1081 ccgcctgtcc gcggctaaac cgggcagcgt tgaacctatc aaggaatgat agatgcagct
     1141 gcggctagac gcactcgaca tgcacctggc gagatcactg tcgccactgt atatcatcgc
     1201 cagcgacgag catctactgg cgctggaagc tgccgccagg attcgtatgg cggcgcgcgc
     1261 caacggctat accgaacgtg aagtgctagt cgtcgagcgc agtttcaaat ggggcgaatt
     1321 gctcgcggcc aatcaatcgc aatcgctgtt tggcgacaag aaactgatcg agctacgtat
     1381 gcccactggc aaacctggca aggacggtgg ccaggtgctg caggactatg ccgcccggct
     1441 gaattcagac catttgacca tcatcagcct acccaggctc gacgtggcta ccgcgaaaac
     1501 ggcctgggtc ggcgccttgc ggcaggccgg catctatatc gatatcccac tggtggaacg
     1561 cgcgcagtta ccagactgga tcggcagccg ccttacctcc cagcagcaaa atgccgaccg
     1621 tccatgcctg gacttcatcg ccgacctcgt cgaaggcaat ctgctggctg cgtatcagga
     1681 gattcaaaag ttggccttgc tctatccggc cggcaagctg acgttcgaac aaattcacgc
     1741 cgccgtgctg aacgtggcgc gctatgacgt cttcaagctc aacgaagcca tactgtcagg
     1801 cgataccgcg cgcctgatgc gcatgatgga tggattgaag ggtgaaggag cagccttgcc
     1861 gctggtgctg tgggccgtca ctgaagaagt gcgcacacta ctcaagctga aatccagcgt
     1921 cacccaaggc aagcaattgg gcatcctgct caaggaacac cgcatctggg gtccacgcga
     1981 aaaactgatg gaaccggcac tgcgccgcat cagcctgtcc atgctacaaa ctgcgttgcg
     2041 ggaagccgcg cagatcgaca ggatggtcaa gggcctgcgc gcacaagctt tttccggcaa
//
LOCUS       DASRDH010000314         3046 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1563526,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000314 DASRDH010000000
VERSION     DASRDH010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1563526"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="VFA14_07245"
     CDS             <1..215
                     /locus_tag="VFA14_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011808702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Appr-1-p processing protein"
                     /protein_id="HZS81205.1"
                     /translation="FESPFGMELLATVDWLLTKEGVAPKVSAVREGLRHWHGDPAAAM
                     RKNRMFDDRAVGIALERLAPGAAVSA"
     gene            complement(271..>3046)
                     /gene="purL"
                     /locus_tag="VFA14_07250"
                     /gene_synonym="purI"
     CDS             complement(271..>3046)
                     /gene="purL"
                     /locus_tag="VFA14_07250"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008328561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HZS81206.1"
                     /translation="RTYEQNVGGNVQGYHKPIMIAGGIGNIKAEHTQKSDLPVGSLLI
                     QLGGPGMRIGMGGSAVSSMAVGANTADLDFDSVQRGNPEMQRRAQEVINACWSMGDDN
                     PILSIHDVGAGGLSNAFPEITNDAGRGAIFDLRKIPLEESGLAPREIWSNESQERYVL
                     ALAPERLPLFRYLCERERCPFAVVGAATQERQLKLIDPGQNNDPVDMPMDVLLGKPPR
                     MQRNVRRVAVALPPVDLTGMDLVEVSHRVLRLPTVADKSFLITIGDRNVGAMTVRDQM
                     VGPWQVPVADCAVTAMGLEGCLGEAMAMGERTPLAVIDAAASGRMAVGEAITNIAAAA
                     IDRIGDIKLSANWMAACGQPGQDAALFDTVKAVGMELCPALGISIPVGKDSLSMRTSW
                     KDDGAVKAVTSPVSLIVSSFAPVQDVRRTLTPQIRTDLGDTVLIVIDLGRGKNRMGAS
                     ALTQVIQQIGNETPDVDSAQDLKGFFTAIQQLNREGRLLAYHDRSDGGLFATLVEMAF
                     AGRSGLSVNLDILSMEGEYASDWGDSKNWAAQVGERRNEMTLRALFSEELGAVIQVRA
                     DQKSEVMNVLRACNLGACSHIIGKPNGRDVIEFMRDAKTIYSQPRSALHRLWSETSWR
                     IARLRDNPDCADAEYERLLDVADPGITPKLSFDPQEDVAAPFINSGARPRVAILREQG
                     VNSHVETAYVMHKSGFESVDVHMSDLIAGRAVLDDFKGLIAVGGFSYGDVLGAGEGWA
                     KTILFNAGLAEQFALFFQRGDTFSLGICNGCQMMSNLKSIIPGAEDWPKFTRNKSERF
                     EARFPMVEIQDSPSIFLQGMAGTQTPIAVAHGEGFADFALTGDISKALVAMRFIDNRG
                     AVTEAYPYNPNGSPQGITFVTTPDGRCNVMMPHAERVFRTVTHSWHPDAWGEDAPWMR
                     MFRNARRWVG"
BASE COUNT          614 a          968 c          875 g          589 t
ORIGIN      
        1 gcttcgaatc acccttcggc atggagttgt tggccacagt ggactggctg ctgacgaagg
       61 aaggcgtggc gccaaaggtt tccgccgtgc gtgaggggct acgccactgg catggcgacc
      121 ctgccgcagc aatgcggaag aacaggatgt tcgacgaccg ggctgtgggc atagcgttgg
      181 aacgactggc gccaggagca gcagtgtcag cttgagtttc gacgccatca gcggcgttgt
      241 gtcagccgct ctcaaaacgt gtttacaatc tcagccaacc cacctgcgcg cattacggaa
      301 catacgcatc cagggcgcat cctcgcccca tgcatcggga tgccacgaat gtgtgacggt
      361 acggaataca cgttcggcgt gcggcatcat cacattgcag cgcccgtccg gcgtcgtcac
      421 aaaggtaatg ccttgcggcg aaccgttcgg attgtatggg taggcttcgg tcacggcgcc
      481 tctgttgtcg atgaaacgca tcgccaccag cgccttgctg atgtcgccag tcaatgcaaa
      541 gtcggcaaaa ccttcaccat gcgcgacggc gatcggcgtt tgcgtgcctg ccatgccttg
      601 caggaagatc gatggcgaat cctgaatctc caccatcggg aaacgcgctt cgaaccgttc
      661 cgacttgtta cgcgtgaact tcggccagtc ttcagcgcct ggaatgatgg acttgaggtt
      721 gctcatcatt tggcagccat tgcagatgcc cagcgaaaat gtatcgccgc gctgaaagaa
      781 cagcgcgaac tgttcggcca gtccagcgtt gaacaaaatg gtcttggccc atccctcacc
      841 cgcccccagc acgtcgccat aagaaaagcc gcctaccgca atcaagcctt tgaaatcatc
      901 caggacggcg cggccggcaa tcaggtcgct catgtggaca tcgaccgatt cgaagccgga
      961 cttgtgcatg acgtaagcgg tttcgacatg cgaattgacg ccctgttcgc gcagaatcgc
     1021 cacgcgcgga cgcgcgccgg agttgatgaa cggcgcggcg acatcctcct gcggatcgaa
     1081 actcagcttc ggcgtgatgc ctggatcggc aacgtcgagc agacgctcgt attcggcatc
     1141 ggcgcagtcc ggattgtcgc gcagacgcgc aatgcgccag ctagtttcac tccacagacg
     1201 atgcaacgca ctgcgcggct ggctgtagat ggtcttggca tcacgcatga attcgatcac
     1261 gtcgcgcccg ttcggcttgc cgatgatgtg gctgcaggcc cccagattgc aggcgcgcag
     1321 cacgttcatg acttccgact tctggtcggc gcgaacctga atcaccgcac ccagttcttc
     1381 actgaacaag gcacgcaaag tcatttcatt gcggcgctcg ccgacctgcg cagcccagtt
     1441 cttggaatcg ccccagtcgg aagcatactc gccttccata ctcaggatgt cgaggttgac
     1501 cgacaagccg ctgcggccgg caaaggccat ctcgaccagc gtcgcgaaca ggccaccatc
     1561 ggaacggtca tgataggcca gcaatcggcc ttccctgttc agttgctgga tcgcagtgaa
     1621 aaagcccttc agatcttgcg cgctgtcgac gtccggcgtt tcgttgccga tctgctgtat
     1681 gacctgcgtc agcgcggatg cacccatgcg attcttgccg cggccgaggt cgatgacgat
     1741 cagcacggtg tcacccaggt ccgtgcggat ttgcggcgtc aatgtccggc gcacatcctg
     1801 caccggcgcg aacgacgaca caatcagtga taccggcgaa gtcacggcct tgaccgcgcc
     1861 atcgtctttc cagctcgtgc gcatcgacaa cgaatccttc ccgaccggaa ttgaaatgcc
     1921 cagcgccgga catagttcca taccgacggc cttcaccgta tcgaacaagg cagcatcctg
     1981 gcccggctga ccgcaagccg ccatccagtt ggccgacagc ttgatatcac cgatcctgtc
     2041 gatggcggcg gcagcgatgt tggtgatcgc ttcgcccacg gccatacgac ccgaggcagc
     2101 agcatcgatc accgccagcg gcgtgcgctc gcccattgcc atcgcctcac cgagacaacc
     2161 ttccagaccc atggcggtaa cggcgcaatc ggccaccggc acctgccacg gtccaaccat
     2221 ctgatcacgc acggtcatcg cgccaacgtt acggtcacca atggtgatca gaaaagattt
     2281 gtcagctacg gtcggcaggc gcaatacgcg atgcgatacc tcgaccagat ccatgccggt
     2341 cagatcgacc ggcggcagcg cgacggcgac gcgcctgaca ttacgctgca tcctgggtgg
     2401 cttgcctagc agcacgtcca tcggcatatc gaccggatcg ttgttttgcc cggggtcgat
     2461 caacttcagt tgccgctcct gcgtcgcggc gccgaccacg gcgaacgggc agcgctcgcg
     2521 ctcgcacagg taacggaaca gcggcagtcg ttccggcgca agcgccaaca cgtaacgctc
     2581 ctgcgattcg ttactccaga tttccctggg cgccagacca ctctcttcca gcggaatctt
     2641 gcgtaagtcg aaaatcgcgc cgcgcccggc gtcattggta atttccggaa aagcattgga
     2701 cagaccgccg gcgccgacat cgtgaatcga cagaatcgga ttgtcgtcgc ccatggacca
     2761 acaagcattg atgacttcct gggcgcggcg ttgcatttcc ggattgccac gctgcaccga
     2821 atcaaagtcc agatcggcgg tattggcgcc gacagccatc gatgacacgg cgctgccgcc
     2881 catgccgatg cgcatgccgg gaccgcccag ctgtatcagc agactgccga ccggcaggtc
     2941 gctcttttgg gtgtgttcgg ccttgatgtt accgataccg ccggcgatca tgatgggttt
     3001 gtggtaaccc tgcacgttgc cgccgacgtt ctgttcgtag gtgcga
//
LOCUS       DASRDH010000315         3061 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1680530,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000315 DASRDH010000000
VERSION     DASRDH010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 3061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3061
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1680530"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="VFA14_07255"
     CDS             complement(<1..209)
                     /locus_tag="VFA14_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZS81207.1"
                     /translation="MNDPQENQAPASVFASETEEIGQVALPGAQLAAQRRARGWSVEH
                     AASRLRFAPRQILALETNNYTALPEL"
     gene            complement(202..1014)
                     /gene="pilW"
                     /locus_tag="VFA14_07260"
     CDS             complement(202..1014)
                     /gene="pilW"
                     /locus_tag="VFA14_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007877272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HZS81208.1"
                     /translation="MRKGVCKSVVALALGSALLLTGCVSSPSDKIAATAGAPIVRLPL
                     RADAQTRATIRLQLAISYFEQRQFAVVLDELTLALQSVPDFADAYSVRALAYMEMGQE
                     QQAEKDFLQAIRLAPSNPDFANNYGWFLCNSDRPGEAIPYFEAAIANRAYQSPGKALT
                     NAGTCSLKRQDVQAAERYLTRAFQYDPTSLATNLNLSRLYAKQREFERARFHISLAAN
                     NHVLNAQALWLAIRIEHKLGNQSAENDLTAQLRRRYPSSSEYAAYQRGAFDE"
     gene            complement(1011..2195)
                     /gene="rlmN"
                     /locus_tag="VFA14_07265"
     CDS             complement(1011..2195)
                     /gene="rlmN"
                     /locus_tag="VFA14_07265"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HZS81209.1"
                     /translation="MMTDFTNLLDLDPAQFITYCGKLGEKPFRARQLQRWIHRYGTAD
                     FAGMTDLAKSLREKLTKHAHVTAPAILSDHTSSDGTRKWLLDVGNGNAVETVFIPEET
                     RGTLCISTQAGCAINCRFCSTGKQGFNRNLGVGEIIGQLWTAEFALRKTRRIAGPKGE
                     RQITNIVMMGMGEPLLNFESVVASLKLMLDDNAYGLSRRRVTLSTSGVVPMIDKLSQE
                     CPVALAVSLHASNDTLRNSLIPLNKKYPLRELMAACRRYLEFAPRDFVTFEYCMLDGI
                     NDSDAHARELVTLVRGGGDGKAAVPCKFNLIPFNPFPGSGLQRSPHARIEAFARILMD
                     AGVVTTVRKTRGDDIDAACGQLAGEVKDRTRVQERMQKMTEYQRQQKSGAGSGRLVEI
                     FE"
     gene            complement(2248..2673)
                     /gene="ndk"
                     /locus_tag="VFA14_07270"
     CDS             complement(2248..2673)
                     /gene="ndk"
                     /locus_tag="VFA14_07270"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HZS81210.1"
                     /translation="MTIERTLSIIKPDAVAQNVIGQIYTRFEDAGLKIIAARMMHLSR
                     AEAEGFYAVHRERPFFRDLVDFMVSGPVIVQVLEGKNAILKHRDLMGATDPKKAEKGT
                     IRADFADSIDANAVHGSDAAETAKTEIAYFFPELNIHSR"
     gene            2882..>3061
                     /locus_tag="VFA14_07275"
     CDS             2882..>3061
                     /locus_tag="VFA14_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007877263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BAX inhibitor (BI)-1/YccA family protein"
                     /protein_id="HZS81211.1"
                     /translation="MNPYPQNVFDTVSTDTVARNRVLRNTYWLLALSMIPTVLGAWLG
                     VQMHFSLFTGSPMLGF"
BASE COUNT          701 a          878 c          808 g          674 t
ORIGIN      
        1 agctccggca aagccgtgta attgtttgtc tccagggcaa gaatctgccg cggtgcaaac
       61 ctcaaccggc ttgccgcgtg ttccacagac cagcctctgg cccggcgttg cgccgccaat
      121 tgcgcgccgg gtagcgcaac ctgccctatc tcttccgttt ccgatgcgaa tacggaggcc
      181 ggcgcctgat tttcctgcgg atcattcatc aaatgctcca cgttgataag ccgcgtattc
      241 cgatgaagac ggatagcgac gacgcagctg cgccgtcaaa tcattttcag ctgactggtt
      301 gccaagttta tgttcaatcc tgatggcaag ccatagcgct tgtgcattca agacatgatt
      361 gtttgccgcc agactgatat gaaaacgggc gcgttcgaac tcgcgctgct tggcatacaa
      421 tctgctcaga ttgaggttgg tcgccaggct ggtcggatca tactgaaaag cgcgggtcaa
      481 ataacgctct gcggcctgca catcctgccg tttcagacta caggtgccgg cattggtcaa
      541 ggctttgccc ggcgactgat atgcccgatt ggcgatggca gcctcgaaat aagggatagc
      601 ctcgccaggg cgatcgctat tgcacaaaaa ccagccatag ttgttggcaa aatccggatt
      661 gctgggtgcc agccggatcg cttgcagaaa atctttctcc gcctgctgct cctgtcccat
      721 ctccatatat gccaacgctc tcacgctata ggcatcagca aaatcgggca cagactgcaa
      781 agccagcgta agctcatcca gcacaacggc aaactgacgt tgctcgaaat agctgatcgc
      841 cagttgaaga cggatagtgg cgcgcgtttg cgcatcagcc cggaggggaa gacgcacaat
      901 cggcgccccg gcggttgccg caattttatc ggatggggac gatacacatc cggttagcag
      961 cagggcagat cccaaagcga gagcgacgac actcttgcac acgccctttc tcattcgaat
     1021 atctccacga ggcggccaga acccgcgccg gatttctgct gacgctgata ttcagtcatt
     1081 ttctgcatac gctcctgcac gcgcgtgcga tccttcacct ctcccgctaa ctggccacag
     1141 gcggcatcga tatcatcgcc gcgtgtcttg cgtaccgtcg tgacaacgcc ggcatccatc
     1201 aggattcgtg caaacgcctc gatgcgcgca tgaggcgagc gctgcaaacc tgaccctgga
     1261 aaaggattga aagggatcag gttgaacttg cacggcacgg ccgccttgcc atcgccgcct
     1321 ccccgcacca gtgtcaccag ttcgcgcgcg tgtgcatcgc tgtcgttgat gccatccagc
     1381 atgcaatatt cgaaagtcac gaagtcacgc ggcgcaaact ccaggtagcg tctgcaagcg
     1441 gccatcagtt cgcgcaacgg atatttttta tttaagggaa tcaaactgtt gcgcaaggta
     1501 tcgttggaag catgcagcga cacggccagc gccacagggc attcctgcga cagcttgtcg
     1561 atcatcggca ccacaccact ggtcgacagc gtcacacggc gacgcgacag gccatacgca
     1621 ttatcgtcaa gcatgagctt caaggaggca acgacagatt caaaattcag caaaggctcg
     1681 cccatgccca tcatcactat gttggtgatc tggcgctctc ccttggggcc tgcgatcctc
     1741 ctggttttac gcaacgcaaa ctcggctgtc cacagctggc cgatgatttc gccgacaccc
     1801 agattgcggt tgaagccttg tttgccggtc gagcagaaac gacaattgat cgcacagccg
     1861 gcctgcgtcg aaatacacag ggtgccgcgg gtttcttcag gaatgaagac ggtttcaacg
     1921 gcgttcccgt ttccgacatc cagcagccat ttgcgggtac cgtcggaaga agtatggtcg
     1981 ctgaggatgg ctggcgccgt cacatgagcg tgcttagtca gcttttcacg cagcgatttg
     2041 gcgagatcgg tcattccggc aaagtcggca gtgccgtatc gatgtatcca gcgttgcaat
     2101 tgcctggcgc gaaatggctt ttcacccagt ttgccgcaat aagtgatgaa ctgcgcagga
     2161 tccagatcca gcagattggt gaaatctgtc atcatggcct ataccatttt ccgtcaagca
     2221 tggatcactc gacatcaaag gcaaaaatca gcgcgaatgg atattcagct ctgggaagaa
     2281 ataggcaatc tcagtcttcg ctgtttctgc cgcgtcggaa ccatgcaccg cattggcatc
     2341 gatcgaatca gcaaaatcag cgcggatcgt gcctttttcc gcctttttcg gatcggttgc
     2401 gcccatcagg tcgcgatgct tcaggatggc atttttacct tccagcacct gcacgatcac
     2461 gggaccggag accatgaagt ccaccagatc cctgaaaaaa ggacgctcgc ggtgaactgc
     2521 gtagaaacct tcagcttccg cacgcgacaa atgcatcatg cgcgcagcga tgattttcag
     2581 gccggcatct tcgaaacggg tgtaaatctg accaatcacg ttttgcgcca ctgcatccgg
     2641 tttgatgatc gacagggtgc gttcgattgt cataggtgaa agctccaatg aaaagaaagg
     2701 gttgaagcat gtcaatcaac atgcaggcaa ccttcgattc tagcataaaa ttaagcttga
     2761 ttctccaggg aatatggcat gcgttccgcg acccggacca tgcggcatcg tgatatgctt
     2821 gcccgcatcg agaaccgtca atgggcggct ctcaattcaa tcaatggcaa ctggaggaaa
     2881 aatgaatcca tacccgcaaa acgtatttga taccgtcagc accgacaccg tcgcacgtaa
     2941 ccgtgtactg cgcaatacct actggctgct tgccttgtcc atgataccga ccgtgctggg
     3001 tgcgtggctg ggcgtgcaga tgcatttcag cctgttcacc ggcagcccga tgctcggctt
     3061 c
//
LOCUS       DASRDH010000316         3704 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_1293618,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000316 DASRDH010000000
VERSION     DASRDH010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 3704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3704
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1293618"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..117)
                     /locus_tag="VFA14_07280"
     CDS             complement(<1..117)
                     /locus_tag="VFA14_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006756831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HZS81212.1"
                     /translation="MIVYEYPFNERIRTLLRLERLHEKFVFFLHQESPLQHHV"
     gene            complement(239..886)
                     /gene="coaE"
                     /locus_tag="VFA14_07285"
     CDS             complement(239..886)
                     /gene="coaE"
                     /locus_tag="VFA14_07285"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007878076.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HZS81213.1"
                     /translation="MSFSSTSTDAPFSIGLTGGIGSGKTTIANLFGALGAAVIDTDVI
                     AHQLSAPGGAAIAAIRAQFGDAFIDAEGGMDRARMRFHVFTDAGARKRLENILHPLIR
                     NAAAQAASAARGDYPIFVVPLLVESQQWRQYVSRILLADCSEEQQIARVMQRDTMSRA
                     QVEAIMAAQATRAQRLAVADDVIENELDTAALLPRIAALHACYLSLAKTNSGEHL"
     gene            complement(883..1752)
                     /locus_tag="VFA14_07290"
     CDS             complement(883..1752)
                     /locus_tag="VFA14_07290"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007878075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HZS81214.1"
                     /translation="MLELLSANAYNMSGSMACAIMASVIGLLTGSFLNVVIHRLPQMM
                     QREADNYLAHESGRSLLHTDRYDLWAPRSACPHCAAALACVDKIPVLSYLLLHGRCRY
                     CRKTIPLRYPVVESFSALLAGWLGWRFGLGVTGLAALAFAFVLITLSFIDAQTGLLPD
                     SLTLPLLWLGLLVNLGNIFSPLRDAVIGAVAGYLVLWMIYWIFRLATGKEGLGYGDFK
                     LLAALGAWLGWQALPIILLLSSCLAAAVGITMVIMRKTAVNQPLSFGPYLAAAGMLAL
                     LYGESFLYSGLYR"
     gene            complement(1752..2969)
                     /locus_tag="VFA14_07295"
     CDS             complement(1752..2969)
                     /locus_tag="VFA14_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005668306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HZS81215.1"
                     /translation="MTADRRRSRRETLYAWEGKDRSGVPMQGEMRAGGATSVSALLRR
                     QGIRTQIIRKKRNQRDKPIRDKELALFTRQLATMLKAGAPLLQAFGIVARSQGNQTFA
                     RLIMRLRADVESGTSLHQAFARHPRHFNSLFCHLVAAGEQAGLLHELLDSLARHQEKM
                     LALKSRIRAALVYPAAIVIVATLVTSIIMIWVVPTFKDVFSSFGAELPLPTRIVIALS
                     DCMTAYWWLLAGIPGSTIGWFVHSWRRSPSVQAKTDRLLLRLPLFGKLIGQATVARWS
                     RTLAVMFGAGVPLIDALQAVGDAAGNAAYADATTAILRQVVAGSGLSAAIERSGMFPG
                     IVAQMVAIGEESGALDQMLIKVADFHEIEVGEAVASLSKLMEPFIMVVLGVVIGALII
                     AMYLPIFKLGSVI"
     gene            complement(2999..>3704)
                     /locus_tag="VFA14_07300"
     CDS             complement(2999..>3704)
                     /locus_tag="VFA14_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005668307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HZS81216.1"
                     /translation="ACLSLLNQPGTNISTVEDPAEIDLPGVNQVNVNERAGLSFPVAL
                     RAFLRQDPDIVMVGEIRDLETADIAVKAAQTGHLVFSTLHTNDAPSTLTRLMNMGVPA
                     FNIASSVIMITAQRLVRRLCSCKQAVRMPAAALREAGFSTADANAHVSGDWTLYHAVG
                     CPRCGSSGYKGRIGVYQVMPISIAIERLILAHASTSEIDAQARSEDVLSLRQAGLKKV
                     REGLTSLTEIIACTNL"
BASE COUNT          848 a         1096 c         1068 g          692 t
ORIGIN      
        1 aacatgatgt tgcagcgggc tttcctgatg cagaaagaac acgaattttt cgtgcagacg
       61 ctccagccgc aatagcgtgc gaatacgctc gttgaaaggg tattcgtata cgatcaaagc
      121 agcatccctt caaagttgaa ccggattcga caatgcgttc gatgaatctg tcttcccata
      181 ttacagatat cgataaaacg catatcattc atgtgattct gacccataca gccgaaaatt
      241 acaaatgctc gccactattt gtttttgcca gcgacaggta gcatgcatgc agcgctgcga
      301 ttcggggcag cagggcagcc gtatcgagct cgttttcaat cacatcgtca gcgacggcga
      361 gacgctgcgc gcgtgtcgcc tgtgctgcca tgatggcctc gacctgcgcg cgcgacatgg
      421 tgtcgcgctg catgacgcgg gcaatctgct gttcttcact acagtctgcc aagagaatac
      481 gcgagacgta ttgacgccat tgctgcgact ccaccaacag cggcaccaca aagatcggat
      541 aatcaccgcg cgcagcggag gcagcctgcg cggcggcgtt gcgaatcagt ggatgaagga
      601 tattttccag gcgttttctg gcaccggcat cggtaaagac atggaaacgc atcctggcgc
      661 gatccatgcc gccttcagca tcgatgaagg catcaccgaa ttgcgcacgg atagctgcaa
      721 tcgcggcgcc gcccggcgcg ctcaactggt gggcaatgac atcagtgtcg atgacagcag
      781 cgcccaatgc accgaacagg ttggcgatag tggtcttgcc gctgccgatg ccgccagtga
      841 gaccaatgga aaaaggcgcg tcggtcgaag tggaagagaa gctcatcgat acaggccgga
      901 atacaggaac gattcaccat acagtagcgc cagcataccc gctgccgcca gatacggacc
      961 gaacgacaat ggctggttca ctgcggtttt ccgcatgatg accatggtga tgccgacggc
     1021 ggcggccagg caggaggaca gcagcaggat gatcggcaac gcttgccagc cgagccacgc
     1081 gcctaacgcc gccagcaatt tgaaatcgcc ataaccgagg ccttccttgc cggtggccag
     1141 cctgaatatc cagtaaatca tccataagac aaggtagccg gccactgccc cgatgacagc
     1201 gtcgcgcagc ggactgaaga tattaccgag attgaccagc aaccccagcc ataacagcgg
     1261 cagcgtcagg ctgtccggca gcaacccggt ttgtgcatcg ataaaactca aggtaatcag
     1321 aacgaaagcg aacgctaacg ccgccagacc ggtcacgcca aggccgaagc gccagcccag
     1381 ccaacccgcc agcaaggcgc tgaagctttc cactaccgga taacgcagcg gaatggtttt
     1441 cctgcaatag cggcatcgac catgcaacag tagatagctc aacacaggaa tcttgtcgac
     1501 gcaggcaagc gcagcggcgc aatgcggaca agccgagcgt ggcgcccaca aatcatagcg
     1561 gtcggtgtgt agcaggctgc ggccggattc atgcgcaaga tagttgtcgg cttcgcgctg
     1621 catcatctgc ggcagccgat ggatcacgac attcaagaaa ctgcctgtca gtagtccgat
     1681 gacagatgcc atgatggcgc atgccatgct gccgctcatg ttgtaagcat tggcgctcag
     1741 tagttccagc atcagataac ggaccccagt ttgaaaatag gcaggtacat ggcaataatc
     1801 agagcgccga tgacgacgcc caacaccacc atgataaaag gttccatgag tttggaaagg
     1861 gatgcgacag cttcgccgac ttctatttca tgaaaatcgg ccactttgat gagcatctgg
     1921 tcgagcgcac cggattcttc accaatggca accatctgag cgacgatacc gggaaacata
     1981 ccgctacgct cgatggcggc gctcaaaccg gaaccggcaa cgacttgccg caggattgcc
     2041 gtggtggcgt cggcataggc ggcattgccg gccgcatcgc cgacggcctg caaggcatcg
     2101 atcagcggca caccggcgcc gaacatgacg gccagggtgc ggctccagcg cgccaccgtc
     2161 gcctgaccga tgagcttgcc gaacaacggc aatcgcagca acagccggtc cgtcttcgcc
     2221 tgcacgctgg gcgaacgccg ccagctatgc acaaaccagc cgatggtact gccgggtatg
     2281 cccgccagca gccaccaata agccgtcatg caatccgaga gcgcgatcac gatgcgggtc
     2341 ggcaacggca actcagcgcc gaagctgctg aagacatcct tgaaagtcgg cactacccag
     2401 atcatgatga tggaagtgac cagggtagcg acgataacga tggctgccgg atataccagc
     2461 gctgcacgta tcctgctctt gagcgccagc atcttttctt gatgacgcgc caggctgtcc
     2521 agcaactcgt gcagcagacc agcttgctcg ccggcggcca ccagatggca aaacaaactg
     2581 ttgaaatggc gcggatgacg cgcgaaagct tgatgcaggc tggtgccgct ttccacatcg
     2641 gcgcgcagac gcatgatcag tcgcgcaaac gtctggttgc cctggctgcg cgcaacgatg
     2701 ccgaaggctt gcaacagcgg cgcgccggcc ttaagcatgg tcgccaactg acgtgtgaac
     2761 agcgccagtt ccttgtcgcg gatgggcttg tcacgctggt ttcttttctt gcggatgatc
     2821 tgtgtccgga tgccctgccg acgcagcagc gcgctgacgc tggtcgcgcc gccggcgcgc
     2881 atttcaccct gcatgggcac gccgctgcga tctttacctt cccaggcata gagtgtttca
     2941 cgtcgtgagc gtcttctgtc tgccgtcatg atgatgctac ttccggaagc gcttaccgtc
     3001 aaagattggt gcaggcgatg atttccgtca ggctggtgag accttcccgg actttcttca
     3061 agcctgcctg ccgtaagctg aggacatctt cactgcgcgc ctgcgcatcg atctccgacg
     3121 tcgaggcatg cgccaaaatg agcctttcga tcgcaatgct gatgggcatg acctgataca
     3181 caccgatgcg tcccttgtaa ccgctgctgc cgcagcgcgg acagccgaca gcgtgataaa
     3241 gcgtccagtc cccggatacg tgggcattgg cgtcagcggt gctgaaacca gcttcccgca
     3301 gggcggcagc gggcatcctc accgcttgct tgcaactgca cagacggcgc accaggcgct
     3361 gagcggtgat catgatgacc gacgacgcga tattgaatgc cggcacgccc atgttcatca
     3421 agcgggtcag cgtcgacggc gcatcgttgg tatgcaaggt ggaaaacacc agatgcccgg
     3481 tctgcgcggc tttgacggca atgtcggcgg tttcgagatc acggatttcg cccaccatga
     3541 caatatccgg atcctggcgc aggaaggcac gcagcgctac cggaaagctc aggccggcac
     3601 gttcgttgac gttgacctga ttgacgccgg gcagatcgat ctcggccgga tcttcgacgg
     3661 tagaaatatt ggtgccaggc tggttcagca gactcaggca ggcg
//
LOCUS       DASRDH010000317         3920 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Herbaspirillum sp. isolate SMAG_U9975 k141_435667,
            whole genome shotgun sequence.
ACCESSION   DASRDH010000317 DASRDH010000000
VERSION     DASRDH010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963503
            Sequence Read Archive: SRR5580765
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Herbaspirillum sp. (soil metagenome)
  ORGANISM  Herbaspirillum sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Oxalobacteraceae; Herbaspirillum.
REFERENCE   1  (bases 1 to 3920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 19:15:11
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,465
            CDSs (total)                      :: 1,444
            Genes (coding)                    :: 1,414
            CDSs (with protein)               :: 1,414
            Genes (RNA)                       :: 21
            tRNAs                             :: 20
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 30
            CDSs (without protein)            :: 30
            Pseudo Genes (ambiguous residues) :: 0 of 30
            Pseudo Genes (frameshifted)       :: 12 of 30
            Pseudo Genes (incomplete)         :: 18 of 30
            Pseudo Genes (internal stop)      :: 4 of 30
            Pseudo Genes (multiple problems)  :: 4 of 30
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3920
                     /organism="Herbaspirillum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435667"
                     /isolate="SMAG_U9975"
                     /isolation_source="soil"
                     /db_xref="taxon:1890675"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..446
                     /locus_tag="VFA14_07305"
     CDS             <1..446
                     /locus_tag="VFA14_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016833906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HZS81217.1"
                     /translation="GGKFNMTDVAAAIGLGQFAHIDAITEKRRSLAWHYFEAFGPDFE
                     TQSGVQLLLADTGNSNWHMYQLVLPERITRAAFMEKMMERQIGIGYHYPPIHLLKLYR
                     ERGFREGMFPIAERIGRRIVSLPMLTAMEKEDVKRVVGAVKSVLA"
     gene            463..1419
                     /locus_tag="VFA14_07310"
     CDS             463..1419
                     /locus_tag="VFA14_07310"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006462632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZS81218.1"
                     /translation="MKPELSVVIPVYNEESSLAGLFERLYPALDALATTYEIIYIDDG
                     SRDRSAAILAGQYRLRPEVTRVILFNGNYGQHMAILAGFEATRGDIVVTLDADLQNPP
                     EEIGNLVAKIREGYDYVGSVRRRRQDAAWRIWASMAVNRLREKTTRIKMTDQGNMLRA
                     YGRNVIDLVNQCREVNTFVPALTYTFARNPTEIIVEHEERAAGKSKYSLYKLIRLNFD
                     LVTGFSLAPLQFFSLLGIGLSFASAALFVLILLRRFLFGSEVQGVFTLFAITFFLIGV
                     ILFGIGLLGEYIGRIYLQVRARPRYVLQTIIEQPPSQNRQSP"
     gene            1416..2333
                     /locus_tag="VFA14_07315"
     CDS             1416..2333
                     /locus_tag="VFA14_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013234068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase"
                     /protein_id="HZS81219.1"
                     /translation="MKAVVFAYHDVGVRCLKTLLARGVDVPLVVTHADSPAENIWFHS
                     VANVCREYGIPCLMPEDPASPQLQEQVQTARPDFIFSFYYRRMLPPSLLAMARHGAYN
                     MHGSLLPKYRGRAPVNWAILHGETETGATLHAMMAKPDAGAIVAQTAVPILPDDTAFE
                     VFGKVVLAAEQTLWRVLPSMLAGCPPTLPNDLSKGSYFSGRAPEDGRIDWGKTAQHIH
                     NLVRAVAPPYPGAFEEISGDKITLARTRMVPANIRLSLESSHRAQDRFFEDDRLYARC
                     GDGNYLQILSIRINDQDVPLNSYLEYMAA"
     gene            2348..3403
                     /locus_tag="VFA14_07320"
     CDS             2348..3403
                     /locus_tag="VFA14_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008328236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-keto-pentose/UDP-xylose
                     synthase"
                     /protein_id="HZS81220.1"
                     /translation="MQKILILGVNGFIGHHLSKRILETTDWHVYGMDMMTDRITGILE
                     NEAYQARMHFFEGDITINREWVEYHVKKCDVILPLVAIATPSTYVSQPLRVFELDFEA
                     NLPIVRSAARYGKHLVFPSTSEVYGLCHDDEFDPEASELVCGPINKPRWIYSCAKQLM
                     DRVIWGYGMQEQLNFTLFRPFNWIGAGLDSIHTPKEGSSRVVTQFFGHIVRGENICLV
                     DGGQQKRTFTYIDDGIDALIKIIANKDGIASGKIYNIGNPANNHSVRELADMMLKLAA
                     EYPEYADSARQVELLETTAAAYYGKGYQDVQNRVPKITNTCAELDWKPGVTMADALRN
                     IFNAYRNQVAAARALVD"
     gene            3447..>3920
                     /locus_tag="VFA14_07325"
     CDS             3447..>3920
                     /locus_tag="VFA14_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008328235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylanase"
                     /protein_id="HZS81221.1"
                     /translation="MPQLTLKIDADTYRGTREGVPNLAQQLLRHGAKASFLFSLGPDH
                     TGWALKQVFRPGFFSKVSRTSVVEHYGIKTLMYGTLLPAPDIGKTCADTMRAVRDAGF
                     ECGIHTWDHRVWQDKVRMRDAAWTQDIMQRSYARFHDIFGHAPVTHGAAGWQMNED"
BASE COUNT          851 a         1249 c         1046 g          774 t
ORIGIN      
        1 tgggcggcaa gttcaacatg accgacgtcg ccgcagccat cggtctgggc cagttcgccc
       61 atatcgacgc catcaccgaa aagcgccgca gcctggcatg gcactacttc gaagccttcg
      121 gccctgattt cgaaacgcaa agcggcgtcc agctgctgct cgcggatact ggcaacagca
      181 actggcacat gtatcagctg gttctgccgg agcgcatcac gcgcgccgcc ttcatggaaa
      241 aaatgatgga gcgccagatc ggcatcggct atcactatcc ccccatccat ttgctcaaac
      301 tctaccgcga gcgcggcttc agggaaggca tgttccccat cgccgaacgc atcggtcgcc
      361 ggatcgtttc gctgccgatg ttgaccgcca tggagaaaga agatgtcaaa cgcgtagtcg
      421 gcgcagtaaa atccgtactc gcataaatct atccaggctt tgatgaaacc agaactttcc
      481 gtcgtcattc ccgtatacaa cgaagaatcc agtctggccg gactgttcga gcgcctctat
      541 ccagcgctgg acgctttggc caccacttac gaaatcattt acatcgacga tggcagccgc
      601 gaccggtcag ccgccattct cgccggacaa taccgcttgc gcccggaggt gacccgcgtc
      661 attctgttca atggcaacta cggtcagcac atggcgattc tggccggctt cgaggccacc
      721 cgcggcgaca tcgtcgtcac cctggacgcc gacctgcaaa atccaccgga agaaatcggc
      781 aatctggtgg ccaagatacg cgaaggctac gactatgtcg gctcggtccg ccgccggcgc
      841 caggacgcgg catggcgcat ctgggcttcc atggccgtga accgcctgcg cgaaaaaacc
      901 acgcgcatca agatgaccga ccagggcaac atgctgcgcg cctatggccg caatgtcatc
      961 gacctggtca atcaatgccg cgaggtcaac accttcgtgc cggctctgac ttataccttc
     1021 gcacgcaatc cgaccgaaat catcgtcgaa cacgaagaac gcgcggctgg caaatccaag
     1081 tactcgctgt acaagctgat ccgcctgaat ttcgatctgg tcaccggctt ttcgctggcg
     1141 ccactgcaat tcttttcgct gctgggcatc ggcctgtcgt tcgcttcggc ggcactgttc
     1201 gtgctgatac tgttgcgccg tttcctgttc ggttccgaag tgcaaggcgt gttcaccctg
     1261 ttcgccatca cctttttcct gatcggcgtg atcctgttcg gcatcggtct tctgggcgaa
     1321 tatatcggcc gcatctattt acaggttcgc gcgcggccgc gctatgtgct gcagacgatc
     1381 atcgagcagc cccccagcca gaacaggcaa agcccatgaa agccgtcgtc ttcgcctatc
     1441 acgatgtcgg cgtgcgctgc ctgaagacac tgctggcgcg cggtgtggac gtaccactgg
     1501 tcgtcaccca tgcagacagt ccggccgaaa atatctggtt ccattccgtc gcgaacgtct
     1561 gccgcgaata cggcattccc tgcctcatgc cagaggatcc cgcatcgccg cagttgcagg
     1621 agcaggtgca gacggcccgg ccggatttca ttttcagctt ctattaccgc cgcatgctac
     1681 cgcccagcct gctggccatg gccagacatg gcgcctacaa tatgcacggc tcattgctgc
     1741 cgaaataccg tggccgcgcg ccggtcaact gggcaatcct gcacggcgaa accgaaaccg
     1801 gcgccacact gcatgccatg atggccaagc cggatgccgg cgccatcgtc gcccagacgg
     1861 cggtgccgat tctgccggac gataccgcct tcgaagtatt cggcaaagtc gtccttgccg
     1921 ccgagcagac tttatggcgc gtcttgccat cgatgctggc cggatgcccg cccacgttgc
     1981 ccaatgattt gagcaaagga agctatttca gcggccgcgc gccggaagac ggccggatcg
     2041 actggggaaa aacagcgcaa catatacata atctggtccg cgccgtcgcg ccgccttatc
     2101 cgggagcctt cgaggaaatc tcaggggaca aaatcacgct ggcgagaacg cgcatggtcc
     2161 cggccaacat ccgactgagc ctggaaagtt cgcaccgcgc ccaggacaga ttcttcgaag
     2221 acgatcgcct gtacgcacgc tgcggcgacg gtaactatct gcaaatcctg agcatacgga
     2281 tcaacgatca ggatgtccct ctcaacagtt atctggaata catggctgcc taagcctcct
     2341 gcacatcatg caaaaaatcc tcattctcgg cgtcaacggt ttcatcggcc accacttgtc
     2401 caaacgcatc ctggaaacca cggactggca tgtctacggc atggacatga tgactgaccg
     2461 catcaccggc attctggaaa atgaggctta ccaagcgcgt atgcacttct ttgaaggcga
     2521 catcacgatc aaccgggagt gggtcgaata ccacgtcaaa aaatgcgacg tgatcctgcc
     2581 gctggtcgcc attgcaacgc cgtcgaccta tgtcagtcag cccttgcgcg tctttgagct
     2641 ggacttcgaa gccaatctgc cgatcgtgcg ttcagctgcc cggtacggca agcatctggt
     2701 gttcccatcg acttcggaag tctatggcct atgtcacgac gatgagttcg accctgaagc
     2761 atccgagctg gtgtgcggtc cgatcaacaa gccgcgctgg atttattcct gcgccaagca
     2821 attgatggac cgcgtgatct ggggctatgg catgcaagag cagctgaact tcacgctgtt
     2881 tcgtccgttc aactggatcg gtgccggcct ggattccatc catacaccga aagaaggcag
     2941 ttcccgcgtc gtgacacaat tcttcggcca catcgtgcgc ggcgaaaaca tctgcctggt
     3001 cgacggtggt cagcaaaagc gcaccttcac ctacatcgac gacggcatcg atgcgctgat
     3061 aaagatcatc gccaacaaag acggcatcgc ctccggcaaa atctacaaca tcggtaatcc
     3121 agccaacaac cattcggtcc gcgagctggc cgacatgatg ctgaagctgg ccgccgagta
     3181 tcctgaatac gcggattcgg ccagacaggt cgaactgctg gaaacgactg cggccgccta
     3241 ctatggcaaa ggctatcagg atgtccagaa ccgtgtgccg aagatcacca atacctgcgc
     3301 agaactggac tggaaaccgg gcgtcaccat ggccgacgcg ctacgcaata ttttcaacgc
     3361 ctaccgcaac caggtcgcgg cagcacgcgc gctggtcgac tgaattttct ccgctcattt
     3421 atttcccatc aagaaaacca gacaccttgc ctcagctcac tctcaaaatc gatgccgaca
     3481 cctaccgcgg cacccgcgaa ggcgttccca atctagctca gcaactgctg cgccatggcg
     3541 ccaaagccag ctttctattt tccctcggcc ccgaccatac gggctgggcg ctcaaacagg
     3601 tatttcgtcc cggttttttc agcaaggtgt cacgcacctc cgtggttgaa cactacggca
     3661 tcaaaacact gatgtacggc acgctgctgc cggcgccgga catcggcaag acctgcgccg
     3721 acaccatgcg cgcggtgcgc gatgccggct tcgaatgcgg catccacacc tgggaccacc
     3781 gggtctggca agacaaggtg cgcatgcgcg atgctgcctg gacgcaggac atcatgcagc
     3841 gctcttatgc ccgcttccac gacatcttcg gccatgcgcc ggtgacgcat ggcgctgccg
     3901 gctggcaaat gaatgaggac
//
